| Entries |
| Document | Title | Date |
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| 20080206873 | COMPLEX - A complex comprising a double-stranded DNA containing a terminal repeated sequence originating in a retrovirus and a DNA having a base sequence which does not occur in this retrovirus and a retrovirus-origin protein component. | 08-28-2008 |
| 20080213901 | Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells - The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells. | 09-04-2008 |
| 20080227205 | Incorporation on Non-Naturally Encoded Amino Acids Into Proteins - The invention provides methods and compositions for in vivo incorporation of non-naturally encoded amino acids into polypeptides by | 09-18-2008 |
| 20080268543 | Process for Producing Cohesive Alcohol Fermentation Yeast and Cohesive Alcohol Fermentation Yeast - The invention provides a method for production of non-uracil-requiring flocculant alcohol-fermenting yeast, comprising a step of introducing the | 10-30-2008 |
| 20080286870 | XYLITOL SYNTHESIS MUTANT OF XYLOSE-UTILIZING ZYMOMONAS FOR ETHANOL PRODUCTION - A strain of xylose-utilizing | 11-20-2008 |
| 20080286871 | Modular genomes for synthetic biology and metabolic engineering - The invention provides methods and compositions for assembling a modular replacement genome in a host microorganism. After such assembly, the host organism's genome is inactivated or ablated to permit full control of host cellular functions by the replacement genome. A modular replacement genome comprises an assembly of nucleic acid fragments, or segments, derived from one or more natural organisms or from synthetic polynucleotides or from a combination of both. Such an assembly, or set, of segments making up a replacement genome comprises a substantially complete set of genes and regulatory elements for carrying out minimal life functions under predefined culture conditions. The invention provides modular genomes having modules that are amenable to facile replacement, deletion, and/or additions. Such modules may be synthetic polynucleotides and may be designed for controlling gene content, excluding of genes that encode inhibitors or otherwise undesirable competing enzymes that divert a host cell from desired metabolic/synthetic processes; modifying codon usage to maximize or minimize protein production; modifying regulatory elements, including promoters, enhancers, repressors, activator, or the like, to modulate gene expression; balancing enzymatic and transport activities to optimize fluxes of substrates, intermediates, and products in metabolic pathways, and like objectives. | 11-20-2008 |
| 20080299661 | CLONING AND/OR SEQUENCING VECTOR - A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison. | 12-04-2008 |
| 20080299662 | Method for Mrna Stabilization - A method to increase the production of a desired chemical compound in a microorganism by introduction of a DNA sequence at the 5′ end of the encoding DNA gene sequence capable of forming a stem loop and capable of increasing the stability of mRNA transcripts from one or more genes, thus stabilized mRNAs, corresponding DNA sequences and microorganisms. | 12-04-2008 |
| 20080318321 | Methods for Generating Hypermutable Microbes - Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria. | 12-25-2008 |
| 20090035863 | Method for the deletion of a large chromosomal region - In order to efficiently delete a large chromosomal region with a length of from several tens to hundreds kb such as a cluster of biosynthesis genes encoding a toxic substance such as Aflatoxin, the present invention provides a method for the deletion of a large chromosomal region comprising transforming a transformant having an increased frequency of homologous recombination due to suppression of a Ku gene, which is a mitosporic filamentous fungus belonging to Trichocomaceae with a vector for the deletion of a chromosomal region, which comprises a pair of homologous region arms having a nucleotide sequence that is homologous with a fragment at either end of said chromosomal region, and deleting the chromosomal region by means of homologous recombination between the resulting transformant and said vector. | 02-05-2009 |
| 20090042301 | TEMPERATURE REGULATED GENE EXPRESSION - A method for regulation of gene expression by variation of temperature uses a riboswitch. The riboswitch includes a 5′-UTR construct of crhC which alters its secondary structure in response to temperature, resulting in a more stable transcript at lower temperatures, permitting translation. At higher temperatures, the transcript is destabilized and functionally inactive. The 5′-UTR construct of crhC may be operatively linked to a promoter and a gene and administered to cells with an expression vector. | 02-12-2009 |
| 20090042302 | Isolated Polynucleotides and Methods of Promoting a Morphology in a Fungus - The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology. | 02-12-2009 |
| 20090081793 | Method for improving acid and low pH tolerance in yeast - A method for increasing tolerance in yeast to organic acids and low pH comprising functionally transforming a yeast with at least one copy of a nucleotide sequence encoding a plasma membrane H | 03-26-2009 |
| 20090124014 | METHOD FOR HOMOLOGOUS RECOMBINATION IN FUNGAL CELLS - The present invention discloses a method to construct fungal cells having a target sequence in a chromosomal DNA sequence replaced by a desired replacement sequence, comprising: providing a DNA molecule comprising a first DNA fragment comprising a desired replacement sequence flanked at its 5′ and 3′ sides by DNA sequences substantially homologous to sequences of the chromosomal DNA flanking the target sequence and a second DNA fragment comprising an expression cassette comprising a gene encoding diphtheria toxin A and regulatory sequences functional in the fungal cell operably linked thereto; transforming the fungal cells with the resulting DNA molecule; growing the cells to obtain transformed progeny cells having the DNA molecule inserted into the chromosome, wherein cells in which the DNA molecule is inserted in the chromosome via a non-homologous recombination event are selectively killed by expression of diphtheria toxin A; and obtaining cells wherein the target sequence in the chromosomal DNA sequence is replaced by the desired replacement sequence. | 05-14-2009 |
| 20090130763 | Method of Controlling Degradation of Protein by Tetracycline Antibiotic - The present invention provides a fusion protein comprising a variant protein of a protein binding to an antibiotic and a target protein having fused thereto, wherein the variant protein is degraded when the antibiotic is not bound but stabilized when the antibiotic is bound in a cell, and the fusion protein is degraded when the antibiotic is not bound but stabilized when the antibiotic is bound in a cell. | 05-21-2009 |
| 20090142843 | Glucose Transport Mutants For Production Of Biomaterial - A method is disclosed for restoring a Glu | 06-04-2009 |
| 20090155912 | METHOD FOR TRANSFER OF MOLECULAR SUBSTANCES WITH PROKARYONTIC NUCLEIC ACID-BINDING PROTEINS - The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation. | 06-18-2009 |
| 20090155913 | COMPOSITIONS COMPRISING PROMOTER SEQUENCES AND METHODS OF USE - Nucleic acid molecules, fragments and variants thereof having promoter activity are provided in the current invention. The invention also provides vectors containing a nucleic acid molecule of the invention and cells comprising the vectors. Methods for making and using the nucleic acid molecules of the invention are further provided. | 06-18-2009 |
| 20090186415 | METHOD FOR PRODUCTION OF CYTOCHROME P450 WITH N-TERMINAL TRUNCATED P450 REDUCTASE - The present invention provides a P450 reductase lacking N-terminal amino acids, as well as a nucleic acid encoding the P450 reductase. When co-expressed with a cytochrome P450, the P450 reductase increases the activity and/or expression of the cytochrome P450 compared to the activity and/or expression of the cytochrome P450 when co-expressed with a wild type P450 reductase. The invention also provides the use of certain promoters to increase expression of cytochrome P450, P450 reductase and/or b5, as well as the use of protease-deficient strains of yeast in which to express the proteins of the present invention. | 07-23-2009 |
| 20090197338 | Method of Increasing the Function of an AAV Vector - A method of correcting singletons in a selected AAV sequence in order to increasing the packaging yield, transduction efficiency, and/or gene transfer efficiency of the selected AAV is provided. This method involves altering one or more singletons in the parental AAV capsid to conform the singleton to the amino acid in the corresponding position(s) of the aligned functional AAV capsid sequences. | 08-06-2009 |
| 20090197339 | In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris - The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as | 08-06-2009 |
| 20090203143 | TRANS-SPLICING MEDIATED PHOTODYNAMIC THERAPY - The present invention provides methods and compositions for conferring selective death on cells expressing a specific target precursor messenger RNA (selective target pre-mRNA). The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) expressed within a cell and mediate a trans-splicing reaction resulting in the generation of a novel chimeric mRNA molecule (chimeric mRNA) capable of encoding a light producing protein or enzyme. Cell death is further mediated by the presence of a photosensitizer which upon photoactivation produces cytotoxicity. | 08-13-2009 |
| 20090215179 | Transgenically preventing establishment and spread of transgenic algae in natural ecosystems - Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of cultivated algae or cyanobacteria to its wild type or to an undesirable, interbreeding related species. as well as preventing the establishment of the transgenic algae or cyanobacteria in natural ecosystems. | 08-27-2009 |
| 20090221078 | ZYMOMONAS WITH IMPROVED ETHANOL PRODUCTION IN MEDIUM CONTAINING CONCENTRATED SUGARS AND ACETATE - Through screening of a | 09-03-2009 |
| 20090246876 | HIGH EXPRESSION ZYMOMONAS PROMOTERS - Identified are mutants of the promoter of the | 10-01-2009 |
| 20090269852 | Novel gene gms 01 - The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. glucose into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose. | 10-29-2009 |
| 20090280569 | Chloramphenicol Resistance Selection in Bacillus Licheniformis - The present invention relates to a modified | 11-12-2009 |
| 20090291504 | CRE COMPLEMENTATION USING LEUCINE ZIPPER - Cre activity is effectively reconstituted from two modified, inactive Cre fragments, both in cell culture and in transgenic mice. Cre reassociation was facilitated by fusing fragments separately to peptides that can form a tight anti-parallel leucine zipper. The co-expressed Cre fusion fragments showed substantial activity in cultured cells. Also disclosed are in vivo techniques for increasing the cell-specificity of gene manipulation, where expression in transgenic mice using individual promoters for each Cre fragment effectively reconstituted Cre activity, and the activation of LoxP recombination in the co-expressing cells. | 11-26-2009 |
| 20090305421 | Recombinant vector for deleting specific regions of chromosome and method for deleting specific chromosomal regions of chromosome in the microorganism using the same - Disclosed herein are a recombinant vector for deletion of specific chromosomal regions and a method for deletion of targeted microbial chromosomal regions using the same. Specifically, the recombinant vector comprises an arabinose-inducible promoter; a gene encoding a protein involved in lambda (λ)-red recombination; a rhamnose-inducible promoter; and a gene encoding the I-SceI endonuclease. The present invention enables a convenient, rapid and markerless successive deletion of specific genes of microbes, as compared to a conventional method. | 12-10-2009 |
| 20090311791 | ALPHA-HEMOLYSIN DELETION MUTATION OF SES-PRODUCING STAPHYLOCOCCUS AUREUS AND CONSTRUCT THEREOF - The present invention relates to an α-hemolysin-deletion mutant of SEs-producing | 12-17-2009 |
| 20090325298 | Pro-Apoptotic Bacteria and Compositions for Delivery and Expression of Antigens - Whole-cell vaccines and methods for enhancing the immunogenicity of cellular microorganisms for use in producing protective immune responses in vertebrate hosts subsequently exposed to pathogenic bacteria or for use as vectors to express exogenous antigens and induce responses against other infectious agents or cancer cells. The present invention involves an additional method of enhancing antigen presentation by intracellular bacteria in a manner that improves vaccine efficacy. After identifying an enzyme that has an anti-apoptotic effect upon host cells infected by an intracellular microbe, the activity of the enzyme produced by the intracellular microbe is reduced by expressing a mutant copy of the enzyme, thereby modifying the microbe so that it increases immunogenicity. | 12-31-2009 |
| 20100035346 | SINGLE PROTEIN PRODUCTION IN LIVING CELLS FACILITATED BY A MESSENGER RNA INTERFERASE - The present invention describes a single-protein production (SPP) system in living | 02-11-2010 |
| 20100035347 | METHOD FOR INCREASING THE SURVIVAL OF BACTERIAL STRAINS OF THE RHIZOBIUM GENUS - The present invention describes a method for increasing the survival of the bacteria of Rhizobium genus, comprising the steps of: making the bacteria to grow in a chemically defined medium; keeping the bacteria in growth stationary phase for a proper period of time; exposing the bacteria to effective quantities of indole-3-acetic acid (IAA). Within the invention scope there is an alternative method to increase the survival of the bacteria of the Rhizobium genus by means of genetic engineering comprising the steps of: making a recombinant vector codifying enzymes able to produce IAA to express in effective way in said bacteria; making the bacteria to grow in chemically defined culture medium; keeping the bacteria in growth stationary phase for a proper period of time. | 02-11-2010 |
| 20100041153 | Expression of Proteins in E. Coli - Plasmid comprising a DNA tag encoding a peptide tag of the sequence MX1(X 2X 3) n X 1 represents K or R; X 2 represents M, S or T; X 3 represents K or R; n represents an integer of 1 or larger; and wherein said DNA is operably-linked to a promoter sequence are provided. | 02-18-2010 |
| 20100062535 | PSOD EXPRESSION UNITS - The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms. | 03-11-2010 |
| 20100068816 | Genetically engineered herbicide resistance for maintaining axenic cultures - This disclosure provides herbicide resistant algae and cyanobacteria. This disclosure also provides a method to cultivate algae and cyanobacteria in axenic cultures without contaminating species. Moreover, this disclosure provides transgenic algal and cyanobacterial cells that are capable of high production in high light intensities as typically applied in cultivation. Furthermore, a novel transformation method is provided for algal cells. | 03-18-2010 |
| 20100087006 | Use of fluorescent protein in cyanobacteria and algae for improving photosynthesis and preventing cell damage - This disclosure provides a method to reduce cell damage caused by near UV light absorption of algal or cyanobacterial cultures. The algal or cyanobacterial cells are transformed to express one or more fluorescent proteins, that absorb the harmful UV or near UV wavelengths and emits wavelengths that are photosynthetically more active. The photosynthetic pigments of the transgenic algal or cyanobacterial cell culture will then absorb the photosynthetically active light emitted by the fluorescent proteins. Accordingly the harmful effects of the UV and near UV radiation are reduced and the photosynthetic activity of the algal or cyanobacterial cells is improved. | 04-08-2010 |
| 20100093095 | COMPOSITIONS AND METHODS FOR ENHANCED BACTERIAL EXOPOLYSACCHARIDE PRODUCTION - The present invention provides nucleic acid sequences and variants thereof capable of modulating exopolysaccharide production in | 04-15-2010 |
| 20100144041 | L-Arabinose Fermenting Yeast - An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. Methods of producing ethanol include utilizing these modified yeast strains. | 06-10-2010 |
| 20100190259 | Ethanol Production - The present invention relates to the production of ethanol as a product of bacterial fermentation. In particular this invention relates to a novel method of gene inactivation and gene expression based upon homologous recombination. The method is particularly useful in connection with species of | 07-29-2010 |
| 20100210017 | COMPOSITIONS AND METHODS FOR ENHANCING TOLERANCE FOR THE PRODUCTION OF ORGANIC CHEMICALS PRODUCED BY MICROORGANISMS - Embodiments herein generally relate to methods, compositions and uses for enhancing tolerance of production of organic acids and alcohols by microorganisms. This application also relates generally to methods, compositions and uses of vectors having one or more genetic element to increase the tolerance of organic acids or alcohols by a microorganism. Certain embodiments relate to compositions and methods of enhancing the tolerance for production of 3-hydroxypropionic acid (3-HP) by bacteria. In some embodiments, compositions and methods relate to regulating the expression of an inhibitory molecule of an enhancing gene to increase production of organic acid by bacteria. | 08-19-2010 |
| 20100261278 | METHOD CAPABLE OF INCREASING COMPETENCY OF BACTERIAL CELL TRANSFORMATION - The invention concerns bacterial strains capable of enhanced transformation efficiencies that are produced by the introduction of the F′ genetic material. The invention also concerns processes for producing transformable competent bacteria with enhanced transformation efficiencies. | 10-14-2010 |
| 20100267147 | SITE-DIRECTED MUTAGENESIS IN CIRCULAR METHYLATED DNA - Site-specific mutation in methylated circular stranded DNA molecules is conferred by mutagenic primer pairs and methylase deficient | 10-21-2010 |
| 20100285592 | SINGLE EXPRESSION VECTOR FOR GENERATION OF A VIRUS WITH A SEGMENTED GENOME - The present invention encompasses an expression vector that is capable of generating a virus from a segmented genome. In particular, a single expression vector may be utilized to produce influenza virus in cultured cells. The expression vector can be delivered in a purified DNA form or by a suitably designed bacterial carrier to cells in culture or to animals. This invention increases the virus generation efficiency, which benefits vaccine development. The bacterial carrier harboring such a plasmid encoding an attenuated virus may be used as a vaccine against corresponding viral disease. | 11-11-2010 |
| 20100297772 | METHOD FOR THE CREATION OF GENETIC DIVERSITY IN VIVO - Disclosed is a method for creating genetic diversity in vivo, comprising the following steps: fragments of at least one wild-type gene sequence are produced, each of said fragments being provided with at least one region which is homologous with a vector that is suitable for in vivo recombination; randomized bridging oligonucleotides of at least one defined oligonucleotide sequence are produced, parts of the bridging oligonucleotide being homologous with at least one fragment of the wild-type gene sequence; and the linearized vector, at least one wild-type fragment, and the randomized bridging oligonucleotide/s are introduced into an in vivo system for homologous recombination. | 11-25-2010 |
| 20100297773 | EXPRESSION CASSETTE, USE OF THE EXPRESSION CASSETTE, VECTOR, HOST CELL, A METHOD FOR PRODUCING A POLYPEPTIDE - The subject matters of invention relate to expression cassette, use of the expression cassette, vector, host cell, a method for producing a polypeptide ensuring its stable expression by the prokaryotic host as well as an use of the expression cassette. The invention enables stable expression of the target polypeptide, in systems where DNA-dependent RNA polymerase recognises promoter regulating synthesis of target protein as well as selection marker, which is required for survival of the host. | 11-25-2010 |
| 20100317115 | METHODS, COMPOSITIONS AND USES FOR ENHANCING CHEMICAL TOLERANCE BY MICROORGANISMS - Embodiments herein concern compositions and methods for enhancing chemical tolerance of biomass conversion by microorganisms. In some embodiments, enhancing tolerance of biomass hydrolysate conversion includes enhancing tolerance to low molecular weight organic compounds. | 12-16-2010 |
| 20100323448 | Methods For Producing Biological Substances In Enzyme-Deficient Mutants Of Aspergillus niger - The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent | 12-23-2010 |
| 20100330678 | PROCESS FOR THE STABLE GENE INTERRUPTION IN CLOSTRIDIA - The present invention is related to a new method for interrupting multiple DNA sequences in Clostridia, even in genes recognized to be essential for the optimal growth of Clostridii by using a counter-selectable marker that would pinpoint the cells that have lost the plasmid and acquired a modified function that permits survival without the interrupted gene. This method is easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes. | 12-30-2010 |
| 20110014708 | NUCLEIC ACID FOR USE IN ALGAE AND USE THEREOF - The present invention provides a modified nucleic acid for expressing | 01-20-2011 |
| 20110020936 | METHOD FOR ELECTROPORATION OF LACTOBACILLUS BUCHNERI WITH NUCLEIC ACID - Electroporation methods for transferring a nucleic acid of interest, including ferulic acid esterase nucleic acid, into bacterial host cells, such as | 01-27-2011 |
| 20110020937 | FLOCCULENT YEAST AND METHOD FOR PRODUCTION THEREOF - It is to provide a novel | 01-27-2011 |
| 20110033938 | SYSTEM FOR INDUCIBLE GENE EXPRESSION IN CHLAMYDOMONAS - The unicellular green alga | 02-10-2011 |
| 20110045592 | METHODS OF GENOME INSTALLATION IN A RECIPIENT HOST CELL - The presently disclosed invention relates to methods of installing a genome isolated from one species (the donor) into suitably prepared cells of a second species (the recipient). Introduction of the donor genetic material into the recipient host cell effectively converts the recipient host cell into a new cell that, as a result of the operation of the donated genetic material, is functionally classified as belonging to the genus and species of the donor genetic material. | 02-24-2011 |
| 20110045593 | Transgenically mitigating the establishment and spread of transgenic algae in natural ecosystems by suppressing the activity of carbonic anhydrase - Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of cultivated algae or cyanobacteria to its wild type or to an undesirable, interbreeding related species, as well as preventing the establishment of the transgenic algae or cyanobacteria in natural ecosystems by suppressing the activity of the carbon concentrating mechanism. | 02-24-2011 |
| 20110053274 | LAC EXPRESSION SYSTEM - Provided herein is a nucleic acid comprising a mutant lac operator operably linked to a gene of interest, a host cell comprising the nucleic acid, and a method of using the host cell to express the gene of interest. Also provided is a recA-mediated cloning method. | 03-03-2011 |
| 20110091977 | Homologous Recombination in an Algal Nuclear Genome - Exemplary transformation methods are provided for introducing deoxyribonucleic acid (DNA) into the nucleus of an algal cell. A transformation construct may be prepared, with the transformation construct having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA, a second sequence of DNA similar to a corresponding second sequence of the nuclear DNA, and a sequence of DNA of interest inserted between the first and second sequences of DNA of the transformation construct. A target sequence of DNA inserted between the first and second corresponding sequences of the nuclear DNA may be transformed, resulting in replacement of the target sequence of DNA with the sequence of DNA of interest. Also provided are exemplary transformation constructs, with some transformation constructs having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA of an algal cell, a second sequence of DNA similar to a corresponding second sequence of nuclear DNA of the algal cell, and a sequence of DNA of interest inserted between the first and second sequences of the transformation construct. | 04-21-2011 |
| 20110104806 | REGULATING THE PRODUCTION OF LONG CHAIN HYDROCARBONS - The invention relates to isolated polypeptides that include amino acid sequences within botryococcene synthase from different algal species. In another aspect, the invention relates to a method for increasing the production level of a botryococcene hydrocarbon molecule in a cell. The method includes increasing expression of a polynucleotide sequence that encodes botryococcene synthase in the cell. In a further aspect, the invention relates to an algal cell having a polynucleotide sequence that is genetically engineered to express a higher level of botryococcene synthase than a corresponding wild type algal cell, wherein the cell produces an increased level of a botryococcene hydrocarbon molecule than a corresponding wild type algal cell. | 05-05-2011 |
| 20110117655 | METHODS AND COMPOSITIONS FOR GENETICALLY MANIPULATING CLOSTRIDIA AND RELATED BACTERIA WITH HOMOLOGOUS RECOMBINATION ASSOCIATED PROTEINS - Methods for effecting homologous recombination in a bacterium of the Clostridia family are described. These methods provide enhanced capability to genetically modify clostridia. | 05-19-2011 |
| 20110124109 | DNA MOLECULES AND METHODS - The present application discloses a DNA molecule comprising a modified Group II intron which does not express the intron-encoded reverse transcriptase but which contains a modified selectable marker gene in the reverse orientation, wherein the marker gene comprises a Group I intron in forward orientation of causing expression in a bacteria cell of the class Clostridia and wherein the DNA molecule comprises sequences that allow for the insertion of the RNA transcript of the Group II intron in the chromosome of a bacterial cell of the class Clostridia. A method of introducing a nucleic acid molecule into a site of a DNA molecule in a bacterial cell of the class Clostridia is also provided. The DNA molecule and the method are useful for making mutations | 05-26-2011 |
| 20110151567 | Recombinant Microorganism - A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of | 06-23-2011 |
| 20110159593 | METHOD OF REDUCING MOTILITY IN BACTERIA BY OVEREXPRESSION OF A GENE OF OI-1 GENOMIC ISLAND - Z0021 was identified as a gene within the O-Island 1 (OI-1) genomic island of verocyto-toxin-producing | 06-30-2011 |
| 20110159594 | NUCLEIC ACIDS, BACTERIA, AND METHODS FOR DEGRADING THE PEPTIDOGLYCAN LAYER OF A CELL WALL - The invention encompasses compositions and methods for degrading the peptidoglycan layer of a cell wall. In particular, the invention encompasses compositions and methods for degrading the peptidoglycan layer of the cell wall of a gram-negative bacterium. | 06-30-2011 |
| 20110159595 | INDUCTION OF FLOCCULATION IN PHOTOSYNTHETIC ORGANSIMS - The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms. | 06-30-2011 |
| 20110189776 | PROKARYOTIC RNAi-LIKE SYSTEM AND METHODS OF USE - Provided herein are methods for inactivating a target polynucleotide. The methods use a psiRNA having a 5′ region and a 3′ region. The 5′ region includes, but is not limited to, 5 to 10 nucleotides chosen from a repeat from a CRISPR locus immediately upstream of a spacer. The 3′ region is substantially complementary to a portion of the target polynucleotide. The methods may be practiced in a prokaryotic microbe or in vitro. Also provided are polypeptides that have endonuclease activity in the presence of a psiRNA and a target polynucleotide, and methods for using the polypeptides. | 08-04-2011 |
| 20110195505 | BACTERIAL STRAINS FOR BUTANOL PRODUCTION - Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the saturated fatty acids content in bacterial cell membrane was increased. Methods for increasing the concentration of saturated fatty acids in the membranes of bacteria that are not natural butanol produces are described whereby tolerance of the bacterial cell to butanol is increased. Saturated fatty acids concentration in the bacterial cell membrane increased upon exogenously feeding saturated fatty acids to cells. Bacterial strains useful for production of butanol are described herein having modified unsaturated fatty acid biosynthetic pathway. | 08-11-2011 |
| 20110195506 | METHOD FOR PRODUCING YEAST EXPRESSED HPV TYPES 6 AND 16 CAPSID PROTEINS - Mosaic VLPs of viral capsid proteins from different virus types are described, as are methods of making the same. Specifically, a diploid yeast strain that coexpresses the L1 and L2 capsid proteins of both HPV-6 and HPV-16 as mosaic VLPs is described. The mosaic VLPs induced the production of conformational antibodies against both L1 proteins upon administration to mice. | 08-11-2011 |
| 20110217780 | TRANSGENIC ALGAE ENGINEERED FOR HIGHER PERFORMANCE - The present disclosure relates to transgenic algae having increased growth characteristics, and methods of increasing growth characteristics of algae. In particular, the disclosure relates to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and a glutamine synthetase | 09-08-2011 |
| 20110223671 | Methods for using positively and negatively selectable genes in a filamentous fungal cell - The present invention relates to methods for using positively and negatively selectable genes in a filamentous fungal cell to delete, disrupt, or insert a gene in a filamentous fungal cell. | 09-15-2011 |
| 20110244575 | METHODS, SYSTEMS AND COMPOSITIONS FOR INCREASED MICROORGANISM TOLERANCE TO AND PRODUCTION OF 3-HYDROXYPROPIONIC ACID (3-HP) - The present invention relates to methods, systems and compositions, including genetically modified microorganisms, adapted to exhibit increased tolerance to 3-hydroxypropionic acid (3-HP), particularly through alterations to interrelated metabolic pathways identified herein as the 3-HP toleragenic pathway complex (“3HPTGC”). In various embodiments these organisms are genetically modified so that an increased 3-HP tolerance is achieved. Also, genetic modifications may be made to provide at least one genetic modification to any of one or more 3-HP biosynthesis pathways in microorganisms comprising one or more genetic modifications of the 3HPTGC. | 10-06-2011 |
| 20110281362 | Electrotransformation of Gram-Positive, Anaerobic, Thermophilic Bacteria - The present invention relates to methods for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation and Gram-positive, anaerobic, thermophilic bacteria transformed by the disclosed methods. The methods employ voltage pulsing schemes that decrease arcing such that increased transformation efficiency and cell viability is observed. The present invention is further directed to a method for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation using recovery/selection temperatures to effect increased transformation efficiency in difficult to transform bacteria. | 11-17-2011 |
| 20110300633 | NOVEL PROMOTER FOR USE IN TRANSFORMATION OF ALGAE - The objective of the present invention is to provide a transformation method that is applicable to a wide variety of species of algae with high efficiency. The promoter of the present invention is characterized in containing a polynucleotide constituting a non-coding region located upstream from a gene encoding a replication-associated protein of a CdebDNA virus or the like. | 12-08-2011 |
| 20110306139 | Mutant Cells Suitable For Recombinant Polypeptide Production - A mutated bacterial cell producing at least one heterologous polypeptide of interest, wherein said cell has a reduced expression-level of a polypeptide comprising an amino acid sequence at least 70% identical to the sequence shown in SEQ ID NO: 2, when compared with an otherwise isogenic but non-mutated cell; methods for producing said mutated cell; and methods for producing a polypeptide of interest using said mutated cell. SEQ ID NO: 2 represents a putative metalloprotease. | 12-15-2011 |
| 20120003742 | RECOMBINANT VIRUS, ESCHERICHIA COLI RETAINING THE SAME AND A PROCESS FOR PRODUCTION THEREOF - To provide a method for producing a recombinant virus retaining the characteristics of the genome of a target virus. | 01-05-2012 |
| 20120034698 | COMPOSITIONS AND METHODS FOR INCREASING OIL CONTENT IN ALGAE - The present invention provides methods and compositions for increasing oil content in algae. More particularly, the present invention relates to enhancement of phytohormone activity in algae, by genetic transformation thereof or by supplementing the algal growth medium with phytohormones, to maximize the production of oil within the algae cells. | 02-09-2012 |
| 20120040465 | MODIFICATION OF THE GENOME OF A LYTIC BACTERIOPHAGE BY IMMOBILIZING SAID BACTERIOPHAGE IN THE HOST BACTERIUM THEREOF - The present invention relates to a method for the reversible immobilization of lytic bacteriophages within their modified bacterial hosts. | 02-16-2012 |
| 20120058563 | PRODUCTION OF 2-KETO-L-GULONIC ACID - The present invention relates to the production of recombinant microorganisms, in particular of the genus | 03-08-2012 |
| 20120077273 | Methods and Compositions for Limiting Viability of a Modified Host Cell Outside of Designated Process Conditions - The invention provides methods and compositions for inhibiting proliferation of a modified host cell outside of a designated process condition. Compositions and methods for providing a host cell having reduced viability when exposed to natural conditions external to a controlled environment are disclosed. | 03-29-2012 |
| 20120094386 | ENGINEERING SALT TOLERANCE IN PHOTOSYNTHETIC MICROORGANISMS - Provided herein are compositions and methods for engineering salt tolerance and producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to be salt tolerant as compared to an unmodified organism and to produce useful products. The methods and compositions of the disclosure are useful in many therapeutic and industrial applications. | 04-19-2012 |
| 20120100616 | METHOD OF DOUBLE CROSSOVER HOMOLOGOUS RECOMBINATION IN CLOSTRIDIA - The invention relates to a method of double crossover homologous recombination in a host Clostridia cell comprising: a first homologous recombination event between a donor DNA molecule and DNA of the host cell to form a product of the first recombination event in the host cell, wherein the donor DNA molecule comprises a codA gene and at least two homology arms; and a second recombination event within the product of the first homologous recombination event, thereby to form a product of the second homologous recombination event in the host cell which is selectable by the loss of the codA gene; and a related vector and altered host cell. | 04-26-2012 |
| 20120115235 | ENHANCED CELLULASE EXPRESSION IN S. DEGRADANS - The invention provides organisms and methods of using and making organisms with enhanced cellulase expression. | 05-10-2012 |
| 20120129264 | Method for Transforming a Bacterium Belonging to the Streptococcus Genus by Natural Competence - The present invention relates to peptides, nucleic acids and methods for transforming a bacterium belonging to the | 05-24-2012 |
| 20120149116 | CELLULOSE DEGRADABLE YEAST AND METHOD FOR PRODUCTION THEREOF - The present invention provides a method for producing a cellulose degradable yeast, comprising the step of co-introducing genes coding for at least two cellulose-degrading enzymes into a yeast host via integration with a yeast δ sequence. According to the invention, a yeast having an improved cellulose degradation ability are provided. | 06-14-2012 |
| 20120156786 | READY-TO-USE ELECTROPORATION CUVETTE INCLUDING FROZEN ELECTROCOMPETENT CELLS - A ready-to-use electroporation cuvette is provided that includes a cuvette, first and second electrodes positioned within the cuvette and electroporation competent cells frozen in a suspension solution within the cuvette, wherein the electroporation cuvette is configured to permit electroporation of the cells when the cells are thawed. The electroporation cuvette may be sealed with a cap that may be color coded to aid the user. | 06-21-2012 |
| 20120190115 | Genes for Enhanced Lipid Metabolism for Accumulation of Lipids - Provided herein are exemplary genes, constructs and methods for the formation of triacylglycerols (TAGs). The exemplary genes include a phosphatic acid phosphohydrolase (PA Hydrolase) gene, a diacylglycerol o-acyltransferase (DAGAT2A) gene, and a phospholipid:diacylglycerol acyltransferase (LROI) gene. | 07-26-2012 |
| 20120190116 | PROCESS FOR CHROMOSOMAL INTEGRATION AND DNA SEQUENCE REPLACEMENT IN CLOSTRIDIA - The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes. | 07-26-2012 |
| 20120196372 | Methods for Reducing Gluconoylation of Proteins - The present invention relates to methods of preventing glyconoylation of polypeptides produced in micororganisms. | 08-02-2012 |
| 20120202292 | Novel method to generate nutritional compounds from microalgae - The present invention relates to the application of genomics technology to generate nutritional compounds in algae, such as microalgae. The present invention discloses a novel method to modify algae to produce a variety of nutritional compounds. Specifically, this method utilizes microalgae genomics technology to manipulate the existing metabolic pathways in the genomes of microalgae and therefore induce the algae to modify secondary metabolic pathways to increase the level of existing nutritional compounds or produce novel nutritional compounds with a variety of commercial applications for improving human and animal nutrition and health. | 08-09-2012 |
| 20120208279 | Transformation of Algal Cells - Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell. | 08-16-2012 |
| 20120208280 | BRUCELLA PHAGE POLYNUCLEOTIDES AND USES THEREOF - An isolated polynucleotide is disclosed which comprises a nucleic acid sequence of a | 08-16-2012 |
| 20120231546 | NOVEL ACETYL CoA CARBOXYLASES - Provided herein are novel ACCases and nucleotides encoding the same, that when introduced into a cell or organism results in an increase and/or accumulation of fatty acids, glycerol lipids, and/or oils in the cell or organism, and/or a change in the types of fatty acids, glycerol lipids, and/or oils that are normally present in the cell or organism. Also provided herein are organisms transformed with the novel ACCases. | 09-13-2012 |
| 20120244621 | TUMOR-SPECIFIC BACTERIAL PROMOTER ELEMENTS - The invention relates to bacterial promoter elements which constitute or which are comprised in promoter regions and which confer tumour specificity to the promoter region activity, resulting in transcription of a transgene, which can be a heterologous or a homologous gene, which transgene is functionally arranged downstream of the promoter region at presence of a host bacterium in tumour tissue, while essentially conferring inactivity of the promoter region and no transcription at presence of the host bacterium in non-tumour tissue. Accordingly, the invention relates to bacterial promoter regions containing these tumour specific promoter elements. | 09-27-2012 |
| 20120244622 | REGULATED GENE EXPRESSION SYSTEMS AND CONSTRUCTS THEREOF - Compositions and methods for nitrogen sensitive regulation of expression of a transcribable nucleic acid molecule. One aspect provides a nitrogen-sensitive expression system that includes a transcription factor region comprising an NtcA binding site and a core promoter region comprising a RuBisCo promoter or a variant or a functional fragment thereof. Another aspect provides a method of transforming a host cell with an expression system. Also provided are expression cassettes, transformed host cells, and kits. | 09-27-2012 |
| 20120252124 | COMPOSITIONS AND METHODS FOR ALTERING ALPHA- AND BETA-TOCOTRIENOL CONTENT USING MULTIPLE TRANSGENES - Preparation and use of isolated nucleic acids useful in altering the oil phenotype of plants are described. Isolated nucleic acids and their encoded polypeptides are described that alter the content of alpha-tocotrienol, beta-tocotrienol, or both, in transformed seeds and oil obtained from the transformed seeds. Expression cassettes, host cells and transformed plants are described that contain the foregoing nucleic acids. | 10-04-2012 |
| 20120270323 | COMPOSITIONS AND METHODS FOR ALTERING ALPHA- AND BETA-TOCOTRIENOL CONTENT - Preparation and use of isolated nucleic acids useful in altering the oil phenotype in plants. Isolated nucleic acids and their encoded polypeptides that alter alpha- and beta-tocotrienol content in seeds and oil obtained from the seeds. Expression cassettes, host cells and transformed plants containing the foregoing nucleic acids. | 10-25-2012 |
| 20120276637 | GENETICALLY ENGINEERED CYANOBACTERIA - The disclosed embodiments provide cyanobacteria spp. that have been genetically engineered to have increased production of carbon-based products of interest. These genetically engineered hosts efficiently convert carbon dioxide and light into carbon-based products of interest such as long chained hydrocarbons. Several constructs containing polynucleotides encoding enzymes active in the metabolic pathways of cyanobacteria are disclosed. In many instances, the cyanobacteria strains have been further genetically modified to optimize production of the carbon-based products of interest. The optimization includes both up-regulation and down-regulation of particular genes. | 11-01-2012 |
| 20120276638 | Methods of Improving the Introduction of DNA Into Bacterial Cells - The present invention relates to methods of improving the introduction of DNA into bacterial host cells. | 11-01-2012 |
| 20120276639 | GENETICALLY ENGINEERED RECOMBINANT ESCHERICHIA COLI PRODUCING L-TRYPTOPHAN HAVING ORIGINALLY L-PHENYLALANINE PRODUCTIVITY, AND METHOD FOR PRODUCING L-TRYPTOPHAN USING THE MICROORGANISM - The present invention relates to a microorganism having L-tryptophan productivity and a method for producing L-tryptophan using the same. More precisely, the present invention relates to the recombinant | 11-01-2012 |
| 20120282700 | MODIFIED FOOD GRADE MICROORGANISM FOR TREATMENT OF INFLAMMATORY BOWEL DISEASE - The present invention relates to microorganisms that express, or have attached to their surface, a TNFα binding polypeptide. Peptides expressed or attached on the surface of microorganism are more resistant to chemical and enzymatic degradation in the gastrointestinal tract. Such microorganisms are capable of binding TNFα and therefore reducing the content of free TNFα and alleviating its pro-inflammatory effects in the gut. The invention also relates to the use of such microorganisms as medicament in the treatment of inflammatory bowel disease. | 11-08-2012 |
| 20120282701 | DNA PROMOTERS AND ANTRHAX VACCINES - The invention is related to intracellularly induced bacterial DNA promoters and vaccines against | 11-08-2012 |
| 20120295356 | APPARATUS AND METHOD FOR GENETICALLY TRANSFORMING CELLS - A fluid containing cells and free genetic material is acoustically coupled to a propulsion surface of a diaphragm. A blast-receiving surface of the diaphragm is acoustically coupled to an explosion chamber in which an explosive material is disposed. An ignition system ignites the explosive material in the explosion chamber to create a blast wave. The diaphragm transfers momentum from the blast wave to the fluid containing cells and free genetic material sufficient to cause the cells to take up the free genetic material. | 11-22-2012 |
| 20120322157 | STRESS-INDUCED LIPID TRIGGER - The present disclosure provides novel proteins that when over expressed in algae result in an increase or change in fatty acid and/or glycerol lipid production and/or accumulation, without a substantial decrease in the growth rate of the alga or the break down of algal components, such as chlorophyll. The present disclosure also describes methods of using the novel proteins to increase or change the production and/or accumulation of fatty acids and/or glycerol lipids in algae. In addition, these proteins are useful tools in obtaining information about the fatty acid and triacyglyceride (TAG) synthetic pathways in algae. | 12-20-2012 |
| 20120329160 | INCREASED OIL CONTENT BY INCREASING YAP1 TRANSCRIPTION FACTOR ACTIVITY IN OLEAGINOUS YEASTS - Transgenic oleaginous yeast having increased oil content comprising increased Yap1 transcription factor activity, wherein the increased oil content is compared to the oil content of a non-transgenic oleaginous yeast, are described herein. The increased Yap1 transcription factor activity results from overexpressing a Yap1 transcription factor, by increasing the interaction between the transcription factor and a protein that is capable of activating the transcription factor, or by a combination thereof. Methods of using these yeast strains are also described. | 12-27-2012 |
| 20130017608 | METHODS OF INCREASING YIELDS OF PLEUROMUTILINS - This invention relates to a novel Pleuromutilin gene cluster and methods of increasing yields of Pleuromutilin produced by | 01-17-2013 |
| 20130023053 | METHODS AND COMPOSITIONS FOR TARGETED MUTAGENESIS IN BACTERIA - This disclosure provides methods and compositions for targeted mutagenesis of specific genes in a bacterial strain. By inducibly over-expressing error-prone polymerases such as Pol IV or Pol V in conjunction with nickase in a bacterial strain, and housing the targeted gene(s) on an episome or plasmid which contains one or more nickase recognition sequences, the targeted gene(s) can be selectively mutated at rates significantly greater than genes contained on the chromosome. The methods disclosed herein are useful for engineering desirable bacterial phenotypes and novel strains, including for example strains useful for treating or degrading waste and/or environmental contaminants, for optimizing bioprocesses, and for converting low-value feed-stock into value-added products. | 01-24-2013 |
| 20130029426 | Dual Inducible System for the cSPP System - The present invention provides a dual inducible system for single protein production, as well as a method of inducing high level protein expression using amino acids. | 01-31-2013 |
| 20130059389 | Method for Producing Kluyveromyces Marxianus Transformant - An object to be solved by the present invention is to provide, for example, a method for producing a | 03-07-2013 |
| 20130065312 | METHOD AND COMPOSITION FOR GENERATING PROGRAMMED CELL DEATH RESISTANT ALGAL CELLS - The present invention provides transgenic algal cells resistant to programmed cell death (PCD) and methods and compositions useful in generating such cells. Specifically, the invention utilizes expression of one or more mammalian anti-apoptotic genes in algal cells to promote resistance to PCD, which is useful for stress tolerance and increased cell viability and biomass production during cultivation. | 03-14-2013 |
| 20130065313 | MOLECULAR BIOLOGY TOOLS FOR ALGAL ENGINEERING - The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention. | 03-14-2013 |
| 20130065314 | ALGAL TRANSFORMATION SYSTEMS, COMPOSITIONS AND METHODS - One aspect of the invention relates to a method for introducing a cargo molecule into an algal cell, the method comprising preparing a composition comprising the cargo molecule and a cell penetrating peptide (CPP) and exposing the algal cell to the composition. The method may also comprise treating the algal cell to disrupt a cell wall of the algal cell. One aspect of the invention relates to a method for transforming an algal cell, the method comprising preparing a composition comprising a nucleic acid molecule and a cell penetrating peptide and exposing the algal cell to the composition. In some embodiments, the CPP is Transportan, Penetratin, an HIV Tat fragment, or a polyarginine, or a variant thereof. | 03-14-2013 |
| 20130109097 | COMPOSITIONS AND METHODS FOR ALTERING ALPHA- AND BETA-TOCOTRIENOL CONTENT USING MULTIPLE TRANSGENES | 05-02-2013 |
| 20130109098 | BIOSECURE GENETICALLY MODIFIED ALGAE | 05-02-2013 |
| 20130115701 | TRANSGENIC PHOTOSYNTHETIC MICROORGANISMS - Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention. | 05-09-2013 |
| 20130130389 | NOVEL PROMOTER FOR USE IN TRANSFORMATION OF ALGAE - The problem to be solved by the present invention is to provide a highly-efficient transformation technology, specifically, a highly-efficient promoter used for transforming algae, a vector comprising the promoter, and a method for transforming algae by using the vector. The promoter according to the present invention is characterized in comprising a polynucleotide constituting a non-coding region located upstream of a gene encoding a structural protein of a ClorDNA virus, and the like. | 05-23-2013 |
| 20130137181 | Ethanol-Resistant Yeast Gene, and Use Thereof - The present invention relates to a gene associated with ethanol tolerance, and yeast strains and uses using the same. The yeast strain of this invention may growth under the condition not only with high-concentration ethanol, preferably 6-15% ethanol, but also in high osmotic pressure, preferably 30-40% glucose or sucrose. The present inventors developed yeast strains resistant to high-concentration glucose and ethanol, suggesting that they would be valuably applied to much effective ethanol production, and also be utilized as a superbacteria having tolerance to various stresses for ethanol production with high efficiency. | 05-30-2013 |
| 20130157370 | Transformation Vector Comprising Transposon, Microorganisms Transformed with the Vector, and Method for Producing L-Lysine Using the Microorganism - The present invention relates to a transformation vector comprising the partial fragments of a gene encoding transposase, a microorganism transformed with the vector, and a method of producing lysine using the microorganism. | 06-20-2013 |
| 20130177989 | Enhanced E. coli for the production of fatty acids and method of producing the same - The invention analyzed a protein sequence using the Udwary-Merski algorithm and identified a tetradomain fragment (DH1-DH2-UMA) which consists of two predicted DH-like domains and two pseudodomains N-terminal to them. This arrangement of domains and pseudodomains is fundamentally the opposite of what is typically observed in the DH cassettes of actinobacterial polyketide synthases or mammalian fatty acid synthases, both of which feature C-terminal pseudodomains. The invention modified | 07-11-2013 |
| 20130183760 | Vectors for directional cloning - The invention provides vectors and methods for directional cloning. | 07-18-2013 |
| 20130183761 | Methods for Incorporating Unnatural Amino Acids in Eukaryotic Cells - The invention relates to a nucleic acid comprising a nucleotide sequence encoding a tRNA orthogonal to a eukaryotic cell, said nucleotide sequence operably linked to a promoter capable of directing transcription by eukaryotic RNA polymerase III. The invention also relates to methods for incorporating unnatural amino acids in eukaryotic cells using same. | 07-18-2013 |
| 20130189787 | Methods, Systems And Compositions Related To Reduction Of Conversions Of Microbially Produced 3-Hydroxyproplonic Acid (3-HP) To Aldehyde Metabolites - The present invention relates to methods, systems and compositions, including genetically modified microorganisms, directed to achieve decreased microbial conversion of 3-hydroxypropionic acid (3-HP) to aldehydes of 3-HP. In various embodiments this is achieved by disruption of particular aldehyde dehydrogenase genes, including multiple gene deletions. Among the specific nucleic acids that are deleted whereby the desired decreased conversion is achieved are aldA, aldB, puuC), and usg of | 07-25-2013 |
| 20130189788 | L-ARABINOSE FERMENTING YEAST - An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains. | 07-25-2013 |
| 20130196441 | ELECTROPORATION ELECTRODE CONFIGURATION AND METHODS - Provided herein are the concept that “singularity-based configuration” electrodes design and method can produce in an ionic substance local high electric fields with low potential differences between electrodes. The singularity-based configuration described here includes: an anode electrode; a cathode electrode; and an insulator disposed between the anode electrode and the cathode electrode. The singularity-based electrode design concept refers to electrodes in which the anode and cathode are adjacent to each other, placed essentially co-planar and are separated by an insulator. The essentially co-planar anode/insulator/cathode configuration bound one surface of the volume of interest and produce desired electric fields locally, i.e., in the vicinity of the interface between the anode and cathode. In an ideal configuration, the interface dimension between the anode and the cathode tends to zero and becomes a point of singularity. | 08-01-2013 |
| 20130217132 | METHODS, COMPOSITIONS AND USE FOR ENHANCING CHEMICAL TOLERANCE BY MICROORGANISMS - Embodiments herein concern compositions and methods for enhancing chemical tolerance of biomass conversion by microorganisms. In some embodiments, enhancing tolerance of biomass hydrolysate conversion includes enhancing tolerance to low molecular weight organic compounds. | 08-22-2013 |
| 20130224864 | FILAMENTOUS FUNGI HAVING AN ALTERED VISCOSITY PHENOTYPE - Described are compositions and methods relating variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications. | 08-29-2013 |
| 20130273660 | STRESS TOLERANT BIFIDOBACTERIA - The present invention relates in general to the field of Bifidobacteria. In particular, the present invention relates to the field of Bifidobacteria with a modulated stress resistance. One embodiment of the present invention is a | 10-17-2013 |
| 20130288377 | COMPOSITIONS AND METHODS FOR MODULATING THE SENSITIVITY OF CELL TO AHAS INHIBITORS - Methods and materials useful for modulating the sensitivity of cells to an inhibitor of acetohydroxyacid synthase (AHAS) are disclosed. For example, nucleic acid molecules encoding AHAS large subunits are disclosed as well as methods for using such nucleic acid molecules to transform microbial cells and plant cells, and to confer modulated sensitivity to AHAS-inhibiting compounds onto such cells. Further provided are materials and methods useful for modulating growth, development, activity, and characteristics of host cells and organisms. | 10-31-2013 |
| 20130309772 | METHOD FOR TRANSFORMATION OF STRAMENOPILE - To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from | 11-21-2013 |
| 20130316457 | Compositions and Methods for Altering Tocotrienol Content - The invention provides isolated nucleic acids and their encoded polypeptides that alter tocol content in seeds. The invention further provides expression cassettes, host cells and transformed plants containing the nucleic acids. The present invention further provides methods for altering tocol content in seeds. | 11-28-2013 |
| 20130316458 | PHOTOSYNTHETIC MICROORGANISMS EXPRESSING THERMOSTABLE LIPASE - The present invention encompasses a photosynthetic microorganism that produces biofuels and biofuel precursors. | 11-28-2013 |
| 20140038294 | SYSTEMS AND METHODS FOR ENGINEERING NUCLEIC ACID CONSTRUCTS USING SCORING TECHNIQUES - Systems and methods are provided for defining a nucleic acid construct for integration at locus L of an organism. Nucleic acid requests are received, each such request specifying a genetic change to L. The request are expanded into component polynucleotides which are then arranged into {AR | 02-06-2014 |
| 20140038295 | BILIVERDIN FROM A NON-ANIMAL SOURCE - Methods for producing biliverdin in a microorganism, methods for producing biliverdin from a non-animal source, cells for producing biliverdin and methods for producing cells for producing biliverdin are disclosed. | 02-06-2014 |
| 20140038296 | METABOLIC ENGINEERING OF MICROBIAL ORGANISMS - Microbial strains with desirable carbohydrate productions characteristics and methods of making and using the same are provided herein. | 02-06-2014 |
| 20140038297 | Genes and Proteins for the Biosynthesis of the Lantibiotic 107891 - The Invention relates to the field of lantibiotics, and more specifically to the isolation of nucleic acid molecules that code for the enzymes required for the biosynthetic pathway of the lantibiotic 107891 and the homologues thereof. | 02-06-2014 |
| 20140045267 | Methods of Introducing Nucleic Acids into Cellular DNA - A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted primase activity or impaired or inhibited or disrupted helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer. | 02-13-2014 |
| 20140045268 | Proteases With Modified Pro Regions - The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include modified polynucleotides that encode modified proteases, which have at least one mutation in the pro region; the modified serine proteases encoded by the modified polynucleotides; expression cassettes, DNA constructs, and vectors comprising the modified polynucleotides that encode the modified proteases; and the bacterial host cells transformed with the vectors of the invention. The methods include methods for enhancing the production of mature proteases in bacterial host cells e.g. | 02-13-2014 |
| 20140051172 | URA5 GENE AND METHODS FOR STABLE GENETIC INTEGRATION IN YEAST - A novel gene encoding | 02-20-2014 |
| 20140093965 | EXPRESSION SYSTEMS AND METHODS OF PRODUCING SPIDER SILK PROTEINS - An expression system, including a host cell, a synthetic spider silk polypeptide-encoding nucleotide sequence, at least one synthetic tRNA molecule-encoding nucleotide sequence or a synthetic serine hydroxymethyl transferase (SHMT)-encoding nucleotide sequence. | 04-03-2014 |
| 20140099719 | METHOD FOR PRODUCING YEAST EXPRESSED HPV TYPES 6 AND 16 CAPSID PROTEINS - Mosaic VLPs of viral capsid proteins from different virus types are described, as are methods of making the same. Specifically, a diploid yeast strain that coexpresses the L1 and L2 capsid proteins of both HPV-6 and HPV-16 as mosaic VLPs is described. The mosaic VLPs induced the production of conformational antibodies against both L1 proteins upon administration to mice. | 04-10-2014 |
| 20140099720 | MICROORGANISM EXPRESSING XYLOSE ISOMERASE - The present invention relates to a transformed microorganism capable of (a) a higher xylose isomerase activity than the equivalent microorganism prior to transformation; and/or (b) a higher growth rate in or on a growth medium comprising xylose than the equivalent microorganism prior to transformation; and/or (c) a faster metabolism of xylose than the equivalent microorganism prior to transformation; and/or (d) a higher production of ethanol when grown anaerobically on xylose as the carbon source than the equivalent microorganism prior to transformation. | 04-10-2014 |
| 20140099721 | FILAMENTOUS FUNGI HAVING AN ALTERED VISCOSITY PHENOTYPE - Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications. | 04-10-2014 |
| 20140113376 | COMPOSITIONS AND METHODS FOR DOWNREGULATING PROKARYOTIC GENES - An isolated polynucleotide is disclosed. The polynucleotide comprises a clustered, regularly interspaced short palindromic repeat (CRISPR) array nucleic acid sequence wherein at least one spacer of the CRISPR is sufficiently complementary to a portion of at least one prokaryotic gene so as to down-regulate expression of the prokaryotic gene and further comprises a nucleic acid sequence encoding at least one CRISPR associated (CAS) polypeptide of a repeat associated mysterious protein (RAMP) family. Uses of the polynucleotides and pharmaceutical compositions comprising the polynucleotides are also disclosed. | 04-24-2014 |
| 20140113377 | GENETIC MANIPULATION AND EXPRESSION SYSTEMS FOR PUCCINIOMYCOTINA AND USTILAGINOMYCOTINA SUBPHYLA - The present invention relates to the application of isolated promoters and synthetic dominant selection constructs and enhancers for gene targeting for efficient production of genetically modified cells in a species selected from the Pucciniomycotina and Ustilaginomycotina subphyla, in particular, species selected from the | 04-24-2014 |
| 20140127816 | FACULTATIVELY ATTENUATED BACTERIAL SPECIES AND METHODS OF PREPARATION AND USE THEREOF - The present invention provides facultatively attenuated bacterial species and methods of preparation and use thereof. The term “facultatively attenuated” as used herein refers to a bacterium which comprises a set of defined recombinant modifications which have substantially no effect on the ability of the bacterium to grow by multiplication when the bacterium is outside of its host organism, but which result in deletion of one or more genes essential for multiplication of the bacterium when the bacterium is introduced into its host organism, for example within host cells of a vaccinate recipient. These recombinant modifications take advantage of regulatory sequences which preferentially induce expression of genes within the mammalian host. | 05-08-2014 |
| 20140127817 | FILAMENTOUS FUNGI HAVING AN ALTERED VISCOSITY PHENOTYPE - Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications. | 05-08-2014 |
| 20140134742 | EFFECTS OF ALTERATION OF EXPRESSION OF THE MtfA GENE AND ITS HOMOLOGS ON THE PRODUCTION OF FUNGAL SECONDARY METABOLITES - Many fungal secondary metabolites are of industrial interest, such as antibiotics, while others are undesirable compounds such as mycotoxins. Overexpression of mtfA enhances production of fungal compounds with applications in the medical field, and overexpression or impaired mtfA expression decreases the production of compounds that negatively affect health/agriculture/economy such as mycotoxins. | 05-15-2014 |
| 20140179007 | EXPRESSION OF GRANULAR STARCH HYDROLYZING ENZYME IN TRICHODERMA - The present invention relates to filamentous fungal host cells and particularly | 06-26-2014 |
| 20140193916 | Electrotransformation of Clostridium pasteurianum - By this invention, for the first time, a method for high-efficiency genetic transformation of the anaerobic bacterium | 07-10-2014 |
| 20140193917 | MODULATION OF MEIOTIC RECOMBINATION - The invention provides methods of modifying the level of expression or functional activity of factors such as enzymes or other catalytic proteins or structural proteins, alone or in concert, to modify the frequency of meiotic homologous recombination involving the exchange of genetic information between non-sister chromatids from homologous maternal and paternal chromosomes. The steps at which modulation may occur include: homologous chromosome pairing, double-strand break formation; resection; strand invasion; branch migration; and resolution. Methods of plant and animal breeding are also provided that utilize the modulation of meiotic homologous recombination. | 07-10-2014 |
| 20140206084 | Primer Set and Method for Homologous Recombination - The present invention provides a primer set used for transformation that imparts a uracil requiring property by deleting or destroying a gene coding for orotidine-5-phosphate decarboxylase in | 07-24-2014 |
| 20140206085 | CASSETTE INCLUDING PROMOTER SEQUENCE OF TARGET GENE AND METHOD OF GENE MANIPULATION USING THE SAME - Provided is a cassette for deleting a target gene comprising (a) a promoter-specific homologous region having a sequence identity to a portion of a promoter region of the target gene, wherein the degree of sequence identity is sufficient to drive homologous recombination therebetween, (b) a marker gene operably linked to the promoter-specific homologous region, and (c) a gene-specific homologous region adjacent to 3′-end of the marker gene and having a sequence identity to at least a portion of the target gene, wherein the degree of sequence identity is sufficient to drive homologous recombination therebetween. | 07-24-2014 |
| 20140206086 | Methods for Obtaining Positive Transformants of a Filamentous Fungal Host Cell - The present invention relates to methods for obtaining positive transformants of a filamentous fungal host cell, comprising: transforming a tandem construct into a population of cells of the filamentous fungal host a tandem construct and isolating a transformant of the filamentous fungal host cell comprising the tandem construct. The present invention also relates to such tandem constructs, filamentous fungal host cells comprising such tandem constructs, and methods of producing multiple recombinant proteins. | 07-24-2014 |
| 20140234976 | COMPOSITIONS AND METHODS FOR CONFERRING HERBICIDE RESISTANCE - The present invention provides a protein, specifically | 08-21-2014 |
| 20140242704 | PEF-TS EXPRESSION UNITS - The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms. | 08-28-2014 |
| 20140248703 | Method for Ordering and Introducing Multiple Genes Into A Genome - Described is a method of introducing multiple nucleic acid molecules into the genome of a cell. The method includes providing a plurality of nucleic acid molecules, each of the plurality of nucleic acid molecules containing (a) a nucleic acid sequence operatively linked to a promoter sequence at the 5′ end of the nucleic acid molecule, and (b) an overlapping sequence at the 3′ end of the nucleic acid molecule. | 09-04-2014 |
| 20140256048 | BACTERIAL XYLOSE ISOMERASES ACTIVE IN YEAST CELLS - Specific polypeptides were identified as bacterial xylose isomerases that are able to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose utilization pathway so that yeast can use xylose in fermentation, such as xylose in biomass hydrolysate. | 09-11-2014 |
| 20140256049 | COW RUMEN XYLOSE ISOMERASES ACTIVE IN YEAST CELLS - Polypeptides were identified among translated coding sequences from a metagenomic cow rumen database, that were shown to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose utilization pathway so that yeast can use xylose in fermentation, such as xylose in biomass hydrolysate. | 09-11-2014 |
| 20140273236 | Use of Clostridial Methyltransferases for Generating Novel Strains - This invention provides isolated polynucleotides encoding DNA Type I methyltransferase and uses thereof for improving transformation efficiencies of exogenous and endogenous plasmid DNA into Clostridial hosts. | 09-18-2014 |
| 20140273237 | Stable Genomic Integration of Multiple Polynucleotide Copies - Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells. | 09-18-2014 |
| 20140273238 | TRANSCRIPTIONAL CONTROL IN ALICYCLOBACILLUS ACIDOCALDARIUS AND ASSOCIATED GENES, PROTEINS, AND METHODS - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 09-18-2014 |
| 20140315313 | FILAMENTOUS FUNGI HAVING AN ALTERED VISCOSITY PHENOTYPE - Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications. | 10-23-2014 |
| 20140329326 | SYNTHETIC GENE CLUSTERS - Methods for making synthetic gene clusters are described. | 11-06-2014 |
| 20140335622 | EXPRESSION VECTOR AND METHOD FOR PRODUCING PROTEIN - Provided are: an expression vector for secreting a protein (Z) to be recovered or a fusion protein having the protein (Z) moiety therein; a method for producing a transformant using the expression vector; the transformant; and a method for producing a protein using the transformant. An expression vector comprising an expression cassette containing a structural gene sequence (y) encoding a protein (Y), a structural gene sequence (z) located downstream from the structural gene sequence (y) and encoding a protein (Z) that is a protein to be recovered, and a promoter sequence and a terminator sequence for expressing a fusion protein containing the protein (Y) moiety and the protein (Z) moiety, characterized in that the protein (Y) is a full-length protein of protein disulfide isomerase 1 (PDI1), a partial protein of PDI1, or a mutant protein of the full-length protein or the partial protein. | 11-13-2014 |
| 20140356962 | NOVEL ATTENUATED POLIOVIRUS: PV-1 MONO-CRE-X - A novel and stable attenuated poliovirus is produced by engineering an indigenous replication element (cre), into the 5′ non-translated genomic region (with inactivation of the native ere element located in the coding region of 2C (mono-crePV), and replacing the nucleic acid sequence of all or part of the capsid coding region (PI) with a substitute PI coding region having reduced codon pair bias. The stably attenuated poliovirus is effective for vaccines and immunization. | 12-04-2014 |
| 20140363892 | REGULATORY ELEMENTS AND USES THEREOF - The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in | 12-11-2014 |
| 20150011007 | Synthetic Transcriptional Control Elements and Methods of Generating and Using Such Elements - Provided herein are nucleic acid constructs that contain a synthetic control element that includes a cis-regulator of translation, and an adapter translation-coupled regulator of transcription. Further provided herein are nucleic acid constructs that contain nucleic acid sequences under the control of the synthetic control elements. Also provided are compositions and methods related to the nucleic acid constructs. | 01-08-2015 |
| 20150031134 | CRISPR-CAS COMPONENT SYSTEMS, METHODS AND COMPOSITIONS FOR SEQUENCE MANIPULATION - The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system. | 01-29-2015 |
| 20150050740 | METHOD FOR INTRODUCING AN EXOGENOUS DNA BY OVERCOMING THE RESTRICTION MODIFICATION BARRIER OF A TARGET BACTERIUM - The present invention discloses a method for introducing an exogenous DNA by overcoming the restriction modification barrier of the target bacterium. The method provided in the present invention comprises the steps of 1) co-expressing all DNA-methyltransferase-encoding genes in the genome of the target bacterium in | 02-19-2015 |
| 20150056706 | VECTOR - The present invention now provides a conditional vector comprising DNA encoding for: (i) an inducible expression cassette comprising an inducible promoter operably linked to a plasmid replication region; and (ii) a selectable marker. | 02-26-2015 |
| 20150056707 | ENGINEERING SALT TOLERANCE IN PHOTOSYNTHETIC MICROORGANISMS - Provided herein are compositions and methods for engineering salt tolerance and producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to be salt tolerant as compared to an unmodified organism and to produce useful products. The methods and compositions of the disclosure are useful in many therapeutic and industrial applications. | 02-26-2015 |
| 20150064791 | Microfluidic-based Gene Synthesis - A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol is applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog. | 03-05-2015 |
| 20150087070 | HIGH EXPRESSION ZYMOMONAS PROMOTERS - Synthetic, derivative promoters for expression of chimeric genes in | 03-26-2015 |
| 20150104875 | ALGAL ELONGASE 6 - Provided herein are exemplary isolated nucleotide sequences encoding polypeptides having elongase activity, which utilize fatty acids as substrates. | 04-16-2015 |
| 20150111298 | Method for Producing Recombinant 11-De-O-Methyltomaymycin - The present invention provides a tomaymycin biosynthetic gene cluster of | 04-23-2015 |
| 20150125959 | Bacterial Mutants with Improved Transformation Efficiency - Provided herein are | 05-07-2015 |
| 20150125960 | ULTRASOUND MEDIATED DELIVERY OF SUBSTANCES TO ALGAE - Embodiments provided herein generally relate to methods and materials for ultrasound-mediated introduction of exogenous substances into microorganisms. Some embodiments relate to methods of introducing an exogenous material into a microorganism such as an algae. The methods can include, for example, providing a microorganism (e.g., algae); providing a population of bubbles that comprise one or more nucleic acid molecules and/or one or more polypeptide or one or more protein molecules; contacting the population of bubbles with the algae; applying ultrasound to the bubbles and algae with sufficient energy to cavitate one or more of bubbles comprising the one or more nucleic acid molecules in proximity to the algae; and maintaining the algae and the one or more burst bubbles comprising the one or more nucleic acid molecules in contact for a period of time sufficient to permit entry of at least one nucleic acid molecule into the algae. | 05-07-2015 |
| 20150140666 | MICROBES WITH CONTROLLED ADHESIVE PROPERTIES - The present invention encompasses a phototrophic microorganism with altered and controlled adhesive properties. One aspect of the present invention encompasses a genetically modified phototrophic microorganism capable of controlled adhesion. The microorganism comprises a recombinant nucleic acid construct, wherein the nucleic acid construct comprises an inducible promoter operably-linked to a nucleic acid encoding an adhesion protein. | 05-21-2015 |
| 20150291948 | NUCLEIC ACIDS USEFUL FOR INTEGRATING INTO AND GENE EXPRESSION IN HYPERTHERMOPHILIC ACIDOPHILIC ARCHAEA - The present invention provides for a novel recombinant or isolated nucleic acid useful for integrating or being maintained in an Archaea or acidophilic hyperthermophilic eubacteria. The nucleic acid encodes a nucleotide sequence that is capable of stably integrating into the chromosome of a host cell, or being maintained as an extrachromosomal element in a host cell, that is an Archea, and a nucleotide sequence of interest. The present invention also provides for an Archaea host cell comprising the nucleic acid stably integrated into the chromosome or maintained episomally in the host cell, and a method of expressing the nucleotide sequence of interest in the host cell and/or directing glycosylation, multimerization, and/or membrane association or integration. | 10-15-2015 |
| 20150299670 | COMBINING GENETIC TRAITS FOR FURFURAL TOLERANCE - Four genetic traits have been identified that increase furfural tolerance in microorganisms, such as ethanol-producing | 10-22-2015 |
| 20150307860 | Acyl-ACP Thioesterase - An acyl-ACP thioesterase consisting of an amino acid sequence of the 115 | 10-29-2015 |
| 20150307862 | Method for improved protein production in filamentous fungi - The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host. | 10-29-2015 |
| 20150307901 | MOLECULAR BIOLOGY TOOLS FOR ALGAL ENGINEERING - The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention. | 10-29-2015 |
| 20150337273 | YEAST STRAINS PRODUCING MAMMALIAN-LIKE COMPLEX N-GLYCANS - Described herein are methods and genetically engineered fungal cells useful for producing target molecules containing mammalian-like complex N-glycans or containing intermediates in a mammalian glycosyiation pathway. | 11-26-2015 |
| 20150353944 | METHOD FOR TRANSFORMING STRAMENOPILE - A method for transforming a stramenopile includes transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus | 12-10-2015 |
| 20150366992 | MULTIMERIC ANTIMICROBIAL PEPTIDE COMPLEX WHICH IS DISPLAYED ON CELL SURFACE - The present invention provides an antimicrobial peptide polymer comprising at least one monomer which is digested by pepsin, a multimeric antimicrobial peptide complex comprising the polymer and a cell surface anchoring motif linked to the polymer, an antimicrobial microorganism displaying the multimeric antimicrobial peptide complex, an antimicrobial composition comprising the same, a method of treating an infectious disease caused by bacteria, yeast or fungi by administering the antimicrobial composition, and a method for producing the antimicrobial microorganism. According to the invention, living microorganisms displaying an antimicrobial peptide on the cell surface thereof may be administered in vivo without having to lyse the microbial cell and isolate and purify the antimicrobial peptide, so that the antimicrobial peptide exhibits antimicrobial activity. Thus, the antimicrobial peptide may be produced at significantly reduced costs so that it may have widespread use. | 12-24-2015 |
| 20150368655 | TRANSFORMED EUGLENA AND PROCESS FOR PRODUCING SAME | 12-24-2015 |
| 20160010070 | METHOD FOR INTRODUCING GENE TO EUGLENA, AND TRANSFORMANT THEREFROM | 01-14-2016 |
| 20160010095 | ENGINEERING MICROORGANISMS | 01-14-2016 |
| 20160017344 | THE TELOMERATOR-A TOOL FOR CHROMOSOME ENGINEERING - The present invention relates to the field of chromosome engineering. More specifically, the present invention provides methods and compositions useful for inducibly linearizing circular DNA molecules in vivo in yeast. In one embodiment, a comprises a nucleic acid encoding a selectable marker, wherein the nucleic acid encoding a selectable marker comprises an intron comprising an endonuclease recognition site flanked by telomere seed sequences. | 01-21-2016 |
| 20160017369 | VECTOR COMPRISING MULTIPLE HOMOLOGOUS NUCLEOTIDE SEQUENCES - The invention relates to vectors comprising two or more homologous nucleotide sequences and methods for generating them. The invention concerns substituting bases in the homologous nucleotide sequences with different bases that do not alter the encoded amino acid sequence. The invention allows for the reduction of intramolecular recombination between homologous nucleotide sequences, in particular in mammalian cells. The invention further relates to nucleotide sequences containing substituted bases. | 01-21-2016 |
| 20160024479 | HIGH TITER RECOMBINANT INFLUENZA VIRUSES WITH ENHANCED REPLICATION IN VERO CELLS - The invention provides a composition useful to prepare high titer influenza viruses, e.g., in the absence of helper virus, which includes internal genes from an influenza virus vaccine strain or isolate, e.g., one that is safe in humans, for instance, one that does not result in significant disease, and genes from vaccine seed virus isolates which include a HA gene segment with a HA2 sequence encoding a HA2 that confers enhanced growth in cells in culture, such as Vero cells. | 01-28-2016 |
| 20160040118 | Genetically Modified Bacteria - The present invention relates to genetically modified methylotrophic bacteria. | 02-11-2016 |
| 20160040173 | Light-Switchable Gene Expression System and the Methods for Controlling Gene Expression in Prokaryotic Bacterium - Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system. The optically controlled gene expression system of prokaryotic bacterium has a quick, effective and powerful induction, is safer than other inducers, is of little or no toxicity, and can control gene expression both spatially and temporally, and can regulate many life processes of prokaryotic bacterium. | 02-11-2016 |
| 20160046902 | Cyanobacteria Having Improved Photosynthetic Activity - This disclosure describes modified photosynthetic microorganisms, including Cyanobacteria, that have a reduced amount of a light harvesting protein (LHP) and contain one or more introduced or overexpressed polynucleotides encoding one or more enzymes associated with lipid biosynthesis, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides. | 02-18-2016 |
| 20160060643 | PREMETHYLATION OF DNA FOR HIGH EFFICIENCY TRANSFORMATION OF CYANOBACTERIA - Methods of pre-methylation of foreign DNA to improve genetic transformation in cyanobacterium. Two Type II methyltransferase-encoding genes, i.e., M (sll0729) and C (slr0214), were cloned from the chromosome of | 03-03-2016 |
| 20160075990 | FUNGAL PROTEASES - The present invention provides fungal proteases and improved fungal strains that are deficient in protease production. | 03-17-2016 |
| 20160076044 | METHOD TO IMPROVE LACTOCOCCUS PRESERVATION - strains with improved preservation characteristics, and improved acid and bile salt tolerance are disclosed. More specifically, a | 03-17-2016 |
| 20160102314 | EXOGENOUS TERMINATORS FOR CONTROLLING FUNGAL GENE EXPRESSION - Provided herein are exogenous terminator sequences for use in fungi cell transcriptional termination. | 04-14-2016 |
| 20160108409 | Method for improved protein production in filamentous fungi - The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host. | 04-21-2016 |
| 20160108432 | DROPLET ACTUATOR FOR ELECTROPORATION AND TRANSFORMING CELLS - The invention provides a droplet actuator designed for performing electroporation on cells in droplets. The invention also provides method and systems for performing electroporation on cells in droplets on a droplet actuator. | 04-21-2016 |
| 20160115488 | CRISPR-CAS COMPONENT SYSTEMS, METHODS AND COMPOSITIONS FOR SEQUENCE MANIPULATION - The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system. | 04-28-2016 |
| 20160115501 | Direct Transfer of Polynucleotides Between Genomes - The present invention provides methods for directly transferring a recombinant polynucleotide of interest from the chromosome of a prokaryotic donor cell to the chromosome of a prokaryotic recipient cell of another species, wherein the polynucleotide of interest comprises at least one coding sequence of interest, preferably one gene of interest and a selectable marker, wherein said polynucleotide of interest in the chromosome of the donor cell is flanked on each side by a polynucleotide region derived from the chromosome of the recipient cell, and wherein the flanking polynucleotide regions are of sufficient size and sequence identity to allow double homologous recombination with the corresponding regions in the chromosome of the recipient cell. | 04-28-2016 |
| 20160130590 | BACTERIAL XYLOSE ISOMERASES ACTIVE IN YEAST CELLS - Specific polypeptides were identified as bacterial xylose isomerases that are able to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose utilization pathway so that yeast can use xylose in fermentation, such as xylose in biomass hydrolysate. | 05-12-2016 |
| 20160137972 | TRANSGENIC ALGAE ENGINEERED FOR HIGHER PERFORMANCE - The present disclosure relates to transgenic algae having increased growth characteristics, and methods of increasing growth characteristics of algae. In particular, the disclosure relates to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and a glutamine synthetase | 05-19-2016 |
| 20160160222 | DNA MOLECULES AND METHODS - The present application discloses a DNA molecule comprising a modified Group II intron which does not express the intron-encoded reverse transcriptase but which contains a modified selectable marker gene in the reverse orientation, wherein the marker gene comprises a Group I intron in forward orientation of causing expression in a bacteria cell of the class Clostridia and wherein the DNA molecule comprises sequences that allow for the insertion of the RNA transcript of the Group II intron in the chromosome of a bacterial cell of the class Clostridia. A method of introducing a nucleic acid molecule into a site of a DNA molecule in a bacterial cell of the class Clostridia is also provided. The DNA molecule and the method are useful for making mutations | 06-09-2016 |
| 20160160224 | CRYPTOSPORIDIUM TRANSFECTION METHODS AND TRANSFECTED CRYPTOSPORIDIUM CELLS - This disclosure describes, in one aspect, a method of transfecting a | 06-09-2016 |
| 20160168582 | ENHANCED PROTEIN EXPRESSION IN BACILLUS | 06-16-2016 |
| 20160177322 | METHODS FOR CLONING AND MANIPULATING GENOMES | 06-23-2016 |
| 20160177338 | METHODS FOR CLONING AND MANIPULATING GENOMES | 06-23-2016 |
| 20160186169 | Effective method for specific gene silencing using artificial small RNA - A nucleic acid molecule comprising a stem-loop structure, a nucleic acid complex comprising the nucleic acid molecule, a composition for delivering a target recognition sequence, and the composition comprising the nucleic acid complex. An artificial small ribonucleic acid of the stem-loop structure stably maintains single-strandedness of a target recognition sequence which interacts with a nucleic acid of interest for a gene of interest of a prokaryote, thereby providing a nucleic acid complex for effective silencing of the gene of interest. | 06-30-2016 |
| 20160251610 | MICROBES WITH CONTROLLED ADHESIVE PROPERTIES | 09-01-2016 |
| 20190144841 | RECOMBINANT POLYNUCLEOTIDE SEQUENCE FOR PRODUCING ASTAXANTHIN AND USES THEREOF | 05-16-2019 |
| 20190144846 | Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium | 05-16-2019 |
