| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 435463000 | Involving general or homologous recombination (e.g., gene targeting, etc.) | 29 |
| 20080206872 | Nucleic acid integration in eukaryotes - The invention relates to methods for directing integration of a nucleic acid of interest towards homologous recombination and uses thereof. The present invention discloses factors involved in integration of a nucleic acid by illegitimate recombination which provides a method of directing integration of a nucleic acid of interest to a predetermined site, whereby the nucleic acid has a homology at or around the predetermined site, in a eukaryote with a preference for non-homologous recombination comprising steering an integration pathway towards homologous recombination. Furthermore, the invention provides a method of directing integration of a nucleic acid of interest to a subtelomeric and/or telomeric region in a eukaryote with a preference for non-homologous recombination. | 08-28-2008 |
| 20080233651 | Method and medicament for inhibiting the expression of a given gene - The present invention relates to the specific inhibition of expression of a target gene in mammals using a short double stranded RNA. The dsRNA is less than 49 nucleotides in length and has a nucleotide sequence which is complementary to at least a part of the target gene. The dsRNAs of the present invention are useful for treating diseases, for example, cancer, viral diseases or neurodegenerative diseases. | 09-25-2008 |
| 20080248576 | Genetic Inhibition of double-stranded RNA - A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods. | 10-09-2008 |
| 20080274550 | Use Of Replicators To Prevent Gene Silencing - Regulatory elements, specifically replicators and transgene constructs containing replicator nucleic acid sequences, are disclosed herein. Methods of using replicators and transgene constructs including replicators to inhibit, delay, or prevent gene silencing are also disclosed herein. | 11-06-2008 |
| 20090137046 | Splicing-Mediated Regulation Of Gene Expression - The present invention relates to methods and compositions for controlling the expression of a target gene, whereby an intron cassette such as INT9, an intronic mec-2-derived element, is incorporated into the target gene and expression of the product of the target gene is conditional upon functional expression of the RNA processing protein, mec-8. | 05-28-2009 |
| 20090170206 | METHOD FOR HOMOLOGOUS RECOMBINATION IN EUKARYOTIC CELLS - We disclose a method to construct eukaryotic cells having a target sequence in a chromosomal DNA sequence replaced by a desired replacement sequence, comprising: modifying a parent eukaryotic cell with a preference for NHR to provide a eukaryotic cell having an increased HR/NHR ratio as compared to the parent cell, providing a DNA molecule comprising a first DNA fragment comprising a desired replacement sequence flanked at its 5′ and 3′ sides by DNA sequences substantially homologous to sequences of the chromosomal DNA flanking the target sequence and a second DNA fragment comprising an expression cassette comprising a gene encoding a selection marker operably linked to regulatory sequences functional in the eukaryotic cell, transforming the modified eukaryotic cells with the DNA molecule, selecting transformed progeny cells having the DNA molecule inserted into the chromosome, deselecting transformed progeny cells having the DNA molecule inserted into the chromosome via NHR by expression of the selection marker, and obtaining cells wherein the target sequence in the chromosomal DNA sequence is replaced by the desired replacement sequence. | 07-02-2009 |
| 20090258425 | GENE KNOCK-DOWN BY INTRACELLULAR EXPRESSION OF APTAMERS - Materials and Methods are provided for target validation by gene knock-down with intracellularly expressed aptamers and siRNAs. The aptamers produced by the materials and methods of the invention are useful in target validation for therapeutics development. | 10-15-2009 |
| 20100009451 | COMPOSITIONS AND METHODS FOR SPECIFICALLY SILENCING A TARGET NUCLEIC ACID - The present invention provides methods modified oligonucleotides and methods of using the modified oligonucleotides for silencing nucleic acids, wherein the nonspecific effects of nucleic acid silencing are reduced. | 01-14-2010 |
| 20100022006 | GAMMA SATELLITE INSULATOR SEQUENCES AND THEIR USE IN PREVENTING GENE SILENCING - Regulatory elements, specifically insulators and transgene constructs containing insulator nucleic acid sequences, are disclosed herein. Methods of using insulators and transgene constructs including insulators to inhibit, delay, or prevent gene silencing are also disclosed herein. | 01-28-2010 |
| 20100197023 | MODIFIED POLYNUCLEOTIDES FOR USE IN RNA INTERFERENCE - Methods and compositions for performing RNA interference comprising a wide variety of stabilized siRNAs suitable for use in serum-containing media and for in vivo applications, such as therapeutic applications, are provided. These siRNAs permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of one or more modifications including orthoesters, terminal conjugates, modified linkages and 2′ modified nucleotides. Uniquely modified siRNAs have been developed that reduces off-target effects incurred in gene-silencing. The modifications include phosphorylation of the first 5′ terminal antisense nucleotide; 2′ carbon modifications of the first and second or first, second, and third 5′ terminal antisense nucleotides; and optionally 2′ carbon modifications of the first and second or first, second, and third 5′ terminal sense nucleotide. Control and exaequo molecules are also provided. siRNA molecules and related control, trackability and exaequo agents with specific stability modifications were developed. | 08-05-2010 |
| 20110008895 | METHODS AND MATERIALS FOR THE REPRODUCIBLE GENERATION OF HIGH PRODUCER CELL LINES FOR RECOMBINANT PROTEINS - The invention lies in the field of production of recombinant gene products in eukaryotic cells. The invention refers to methods and materials for the fast and reproducible generation of production cells lines suitable for large scale production of recombinant gene products. The invention encompasses specific vector systems, genetic engineered host-cells and methods of use. | 01-13-2011 |
| 20110097804 | Targeted gene modification by parvoviral vectors - This invention provides methods for obtaining targeted gene modification in vertebrate cells using parvoviral vectors, including adeno-associated virus (AAV). The parvoviral vectors used in the methods of the invention are capable of targeting a specific genetic modification to a preselected target locus in a cellular genome by homologous pairing. | 04-28-2011 |
| 20110281361 | Linear donor constructs for targeted integration - Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for integration of an exogenous sequence into a specified region of interest in the genome of a cell. | 11-17-2011 |
| 20110300632 | METHOD OF DIRECTING THE EVOLUTION OF AN ORGANISM - The present disclosure relates to a method of directing the evolution of an organism by modifying the mutation rate of an organism. The increase in genetic diversity may be used to facilitate the selection of a desired hereditary trait in an organism. | 12-08-2011 |
| 20120142110 | Method of Genetically Altering and Producing Allergy Free Cats - A transgenic cat with a phenotype characterized by the substantial absence of the major cat allergen, Fel d I. The phenotype is conferred in the transgenic cat by disrupting the coding sequence of the target gene with a specialized construct. The phenotype of the transgenic cat is transmissible to its offspring. | 06-07-2012 |
| 20120178169 | TAL EFFECTOR-MEDIATED DNA MODIFICATION - Materials and Methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; “TALENS”) are provided. | 07-12-2012 |
| 20120252123 | NUCLEIC ACID SILENCING SEQUENCES - The present invention features compositions and methods for introducing, into cells, nucleic acids whose expression results in chromosomal silencing. The nucleic acids are targeted to specific chromosomal regions where they subsequently reduce the expression of deleterious genes, or cause the death of deleterious cells. Where the nucleic acid sequence is a silencing sequence, it may encode an Xist RNA or other non-coding, silencing RNA. Accordingly, the present invention features, inter alia, nucleic acid constructs that include a transgene (e.g., a silencing sequence encoding an Xist RNA or other non-coding RNA that silences a segment of a chromosome); first and second sequences that direct insertion of the silencing sequence into a targeted chromosome; and, optionally, a selectable marker. | 10-04-2012 |
| 20120282698 | METHOD FOR HOMOLOGOUS RECOMBINATION IN EUKARYOTIC CELLS - A method to construct eukaryotic cells having a target sequence in a chromosomal DNA sequence replaced by a desired replacement sequence is disclosed. | 11-08-2012 |
| 20130130388 | Methods of Modifying Eurakyotic Cells - A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification. | 05-23-2013 |
| 20130295675 | METHOD FOR INSERTING GENETIC MATERIAL INTO GENOMIC DNA - The present invention provides reagents and methods for improved homologous recombination. | 11-07-2013 |
| 20140335621 | GENE TARGETING VECTOR, AND METHOD FOR USING SAME - Provided is a gene targeting vector that enables highly efficient gene targeting. The gene targeting vector has a structure comprising a positive selection marker flanked by a DNA homologous to a 5′-upstream region of a target site and a DNA homologous to a 3′-downstream region of the target site, wherein a splice acceptor site and a DNA sequence allowing for bicistronic expression are added 5′-upstream of the positive selection marker, and another splice acceptor site is also added 5′-upstream of the DNA homologous to the 5′-upstream region of the target site. | 11-13-2014 |
| 20150307561 | ENGINEERED TRANSCRIPTION ACTIVATOR LIKE EFFECTOR (TALE) PROTEINS - Disclosed herein are methods and compositions for design and use of engineered TALEs. | 10-29-2015 |
| 20150344908 | METHOD OF GENETICALLY ALTERING AND PRODUCING ALLERGY FREE CATS - A transgenic cat with a phenotype characterized by the substantial absence of the major cat allergen, Fel d I. The phenotype is conferred in the transgenic cat by disrupting the coding sequence of the target gene with a specialized construct. The phenotype of the transgenic cat is transmissible to its offspring. | 12-03-2015 |
| 20160017371 | STEM CELL GENE TARGETING - The invention provides a method for generating a transgenic eukaryotic cell population having a modified human Rosa26 locus, which method includes introducing a functional DNA sequence into the human Rosa26 locus of starting eukaryotic cells. Also provided are targeting vectors useful in the method, as well as a cell population and a transgenic non-human animal comprising a modified human Rosa26 locus. Finally, the invention provides an isolated DNA sequence corresponding to the human Rosa26 locus. | 01-21-2016 |
| 20160145644 | TARGETED GENOMIC MODIFICATION WITH PARTIALLY SINGLE-STRANDED DONOR MOLECULES - Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell. | 05-26-2016 |
| 20160168594 | ONCOGENIC MODELS BASED ON DELIVERY AND USE OF THE CRISPR-CAS SYSTEMS, VECTORS AND COMPOSITIONS | 06-16-2016 |
| 20160177290 | METHODS AND COMPOSTIONS FOR TARGETED GENOMIC DELETION | 06-23-2016 |
| 20160194665 | METHODS AND COMPOSITIONS FOR INACTIVATING ALPHA 1,6 FUCOSYLTRANSFERASE (FUT8) GENE EXPRESSION | 07-07-2016 |
| 20180022831 | NANOPARTICLE-PROTEIN COMPLEX FOR INTRACELLULAR PROTEIN DELIVERY | 01-25-2018 |
