| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 435449000 | Fusion of cells | 26 |
| 20100068813 | APOPTOTIC CELL-MEDIATED TRANSFECTION OF MAMMALIAN CELLS WITH INTERFERING RNA - Mammalian host cells for use in a cell-mediated tranfection process, which contain an RNAi molecule and an expression vector for a pro-apoptotic protein. The method includes inducing apoptotic cell (AC) death in mammalian cells that contain an RNAi molecule capable of downregulating a chosen target gene. Living cells expressing the target gene are then exposed to the ACs. The ACs are processed by the living cells, and the RNAi molecule in the ACs downregulates the expression of the target gene in living cells. | 03-18-2010 |
| 20100178702 | EXTRACELLULAR VESICLES FROM NON-PATHOGENIC AMOEBAE USEFUL AS VEHICLE FOR TRANSFERRING A MOLECULE OF INTEREST TO AN EUKARYOTIC CELL - The invention relates to membrane vesicles from non-pathogenic amoeba, in particular from the amoeba | 07-15-2010 |
| 20110020934 | Fusion Partner for Production of Monoclonal Rabbit Antibodies - The invention provides a rabbit-derived immortal B-lymphocyte capable of fusion with a rabbit splenocyte to produce a hybrid cell that produces an antibody. The immortal B-lymphocyte does not detectably express endogenous immunoglobulin heavy chain and may contain, in certain embodiments, an altered immunoglobulin heavy chain-encoding gene. A hybridoma resulting from fusion between the subject immortal B-lymphocyte and a rabbit antibody-producing cell is provided, as is a method of using that hybridoma to produce an antibody. The subject invention finds use in a variety of different diagnostic, therapeutic and research applications. | 01-27-2011 |
| 20120129260 | METHOD FOR PRODUCING PLURIPOTENT STEM CELLS - Methods are provided for producing a human embryo capable of developing to the blastocyst stage. The method includes transferring a human somatic cell genome into a mature human oocyte by nuclear transfer and activating the oocyte, without removing the oocyte genome. Pluripotent human embryonic stem cells, and methods of obtaining these, are also provided. | 05-24-2012 |
| 20120142106 | Method Of Forming Dendritic Cells From Embryonic Stem Cells - This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers. | 06-07-2012 |
| 20120322156 | MEMBRANE FUSION PROTEINS DERIVED FROM REOVIRUS - In accordance with the present invention, a family of membrane fusion protein and polynucleotides encoding the proteins have been identified. The proteins and nucleotides are derived from the family Reoviridae. Two membrane fusion proteins have been isolated from reoviruses isolated from poikilothermic hosts: the p14 protein from reptilian reovirus (RRV) isolated from python, and the p16 protein from | 12-20-2012 |
| 20130034906 | METHODS FOR IN VITRO OOCYTE MATURATION - The invention relates to methods for assisted reproduction technology in mammals. Specifically the invention relates to methods for in vitro mammalian oocyte culture and oocyte maturation, in vitro fertilization, and in vitro embryo development. | 02-07-2013 |
| 20130065307 | Use of RNA interference for the creation of lineage specific ES and other undifferentiated cells and production of differentiated cells in vitro by co-culture - Methods for making human ES cells and human differentiated cells and tissues for transplantation are described, whereby the cells and tissues are created following somatic cell nuclear transfer. The nuclear transfer donor is genetically modified prior to nuclear transfer such that cells of at least one developmental lineage are de-differentiated, i.e., unable to develop, thereby resolving the ethical dilemmas involved in reprogramming somatic cells back to the embryonic stage. The method concomitantly directs differentiation such that the desired cells and tissues may be more readily isolated. | 03-14-2013 |
| 20130177987 | METHODS FOR DEVELOPING ANTIGEN-SPECIFIC ANTIBODY-PRODUCING CELL LINES AND MONOCLONAL ANTIBODIES - Disclosed are methods for producing class-switched, affinity-matured antibodies which include enriching an immunized cell population for GL7-positive cells and activating the enriched cells. The methods may be used to improve the efficiency of obtaining immortalized antigen-specific plasma cells or to improve the quality of molecularly cloned Ig heavy and light chains. | 07-11-2013 |
| 20140234968 | PRODUCTION OF PARTHENOGENETIC STEM CELLS AND PATIENT-SPECIFIC HUMAN EMBRYONIC STEM CELLS USING SOMATIC CELL NUCLEAR TRANSFER - Immunocompatible pluripotent stem cells (pSCs), which include cells compatible with different patient populations or patient-specific cells, find wide application in regenerative medicine therapies. Described herein are immunocompatible pSCs generated using techniques such as parthenogenesis resulting in cells possessing desired haplotypes of reduced zygosity, antigenically compatible with multiple patient populations, or nuclear transfer allowing generation of patient-specific cells. Methods described herein related to parthenogenesis, nuclear transfer, or pSC cell line generation. Also described herein are compositions of immunocompatible pSCs and cell lines generated by the aforementioned techniques. | 08-21-2014 |
| 20140248700 | Method of Differentiation of Morula or Inner Cell Mass Cells and Method of Making Lineage-Defective Embryonic Stem Cells - An improved method of producing differentiated progenitor cells comprising obtaining inner cell mass cells from a blastocyst and inducing differentiation of the inner cell mass cells to produce differentiated progenitor cells. The differentiated progenitor cells may be transfected such that there is an addition, deletion or alteration of a desired gene. The differentiated progenitor cells are useful in cell therapy and as a I source of cells for the production of tissues and organs for transplantation. Also provided is a method of producing a lineage-defective human embryonic stem cell. | 09-04-2014 |
| 20160024528 | SOMATIC CELL NUCLEAR TRANSFER METHODS - The present invention provides methods for making reconstructed diploid human oocytes comprising the diploid genome of a human somatic cell, and also methods for making human nuclear transfer embryos, human embryonic stem cells, and human differentiated cells therefrom. The present invention also provides reconstructed human oocytes, human nuclear transfer embryos, human embryonic stem cells, and differentiated cells made using such methods, as well as compositions and kits useful in performing such methods. | 01-28-2016 |
| 20160060602 | FUSION MIXTURE - The invention relates to a fusion mixture for the lipid-containing membrane modification of an arbitrary target membrane, a cell membrane, a constituent of a cell membrane or a cell membrane separated from remaining cell constituents, in vivo or in vitro, comprising a positively charged amphipathic molecule A and an aromatic molecule B, wherein the molecule of type A and the molecule of type B are present at a ratio A:B of 1:0:02 to 1:2 mol/mol. | 03-03-2016 |
| 435450000 | Employing electric current | 5 |
| 20120276635 | FLUIDIC DEVICE - A fluidic device for cell electroporation, cell lysis, and cell electrofusion based on constant DC voltage and geometric variation is provided. The fluidic device can be used with prokaryotic or eukaryotic cells. In addition, the device can be used for electroporative delivery of compounds, drugs, and genes into prokaryotic and eukaryotic cells on a microfluidic platform. | 11-01-2012 |
| 20130089929 | MICRODEVICE FOR FUSING CELLS - A microdevice for fusing cells, the microdevice including: a substrate; a first electrode array including a plurality of first electrodes, and disposed on the substrate; a microwell array including a plurality of microwells formed respectively at locations corresponding to the plurality of first electrodes, and disposed on the first electrode array; a second electrode disposed above the plurality of microwells, and including a microchannel having a predetermined height; inlet and outlet holes mutually spaced apart from the microchannel; and a power supply unit applying voltage to the plurality of first electrodes and the second electrode. Accordingly, a cell trapped in the microwell and a cell disposed on the microwell are aligned in a line between the first and second electrodes, and thus the two cells having different traits are smoothly fused in a one-to-one manner when an electric shock is applied to the two cells. | 04-11-2013 |
| 20130089930 | MICRODEVICE FOR FUSING CELLS - A microdevice for fusing cells including: a microchannel layer including a main microchannel and a plurality of sub-microchannels branched from one end of the main microchannel; a plurality of first electrodes formed on one side of the main microchannel; a plurality of second electrodes formed on the other side of the main microchannel and each second electrode facing the each of the first electrodes; a thin film disposed on the microchannel layer and covering the main microchannel; an upper cover including an air inflow passage for connecting a top of the thin film and the outside of the microdevice; and a power supply unit for applying voltage to the plurality of first and second electrodes. | 04-11-2013 |
| 20130089931 | MICRODEVICE FOR FUSING CELLS - A microdevice for fusing cells including: a membrane with a plurality of pores having a diameter smaller than the smallest diameter among the first kind of cells and second kind of cells; a first chamber where the first cell is located and a second chamber where the second cell is located, wherein the membrane is disposed therebetween; a first electrode combined to the first chamber; a second electrode combined to the second chamber; and a power generator applying a voltage to the first and second electrodes. Accordingly, the first and second cells across the membrane may be arranged in a one-to-one manner between the first and second electrodes, and thus the first and second cells having different traits may be smoothly fused in a one-to-one manner when electric signals are sequentially applied thereto. | 04-11-2013 |
| 20140017791 | Combining Biological Micro-Objects - Two or more biological micro-objects can be grouped in a liquid medium in a chamber. Grouping can comprise bringing into and holding in proximity or contact the micro-objects in a group, breaching the membrane of one or more of the micro-objects in a group, subjecting one or more of the micro-objects in a group to electroporation, and/or tethering to each other the micro-objects in a group. The micro-objects in the group can then be combined into a single biological object. | 01-16-2014 |
| 435451000 | One of the fusing cells is a human antibody-producing cell | 2 |
| 20100029002 | ENHANCEMENT OF HYBRIDOMA FUSION EFFICIENCIES THROUGH CELL SYNCHRONIZATION - The present invention provides methods of enhancing hybridoma fusion efficiencies through cell synchronization of the fusion partners, in order to aid in production of antibodies. | 02-04-2010 |
| 20120088304 | METHODS OF GENERATING CELLS EXHIBITING PHENOTYPIC PLASTICITY - The present invention relates to cellular differentiation and, in particular to hybrid cells that exhibit phenotypic plasticity and methods for producing these cells. The invention also relates to methods for generating specific cells of a desired phenotype. The invention still further relates to methods of producing hybrid cells with a capacity to de-differentiate into an earlier progenitor state. The invention further contemplates the use of hybrid cells in a range of applications, for example tissue generation. | 04-12-2012 |
| 435454000 | One of the fusing cells is a microorganism (e.g., prokaryote, fungus, etc.) | 6 |
| 20110165680 | CLEAN GENOME BACTOFECTION - Methods for introducing and expressing genes in animal cells are provided comprising infecting the animal cells with live invasive reduced-genome bacteria comprising a eukaryotic expression cassette comprising said gene. Also provided are methods for producing a pluripotent stem (iPS) cell from a mammalian somatic cell comprising infecting the somatic cell with live invasive reduced-genome bacteria comprising one or more eukaryotic expression cassettes comprising at least a gene encoding the transcription factor Oct3/4 and a gene encoding a member of the SRY-related HMG-box (Sox) transcription factor family. | 07-07-2011 |
| 20110229968 | ETHANOL RESISTANCE SACCHAROMYCES CEREVISIAE GP-01 BY PROTOPLAST FUSION, METHOD FOR MANUFACTURING THEREOF, METHOD FOR MANUFACTURING YEAST CONTAINING HIGH CONTENT OF BIO ORGANIC GERMANIUM BY USING SACCHAROMYCES CEREVISIAE GP-01 AND HIGH WATER SOLUBLE SODIUM METAGERMANATE AS A GERMANIUM SOURCE - A biologically pure culture of | 09-22-2011 |
| 20110281360 | MULTI-CHAIN EUKARYOTIC DISPLAY VECTORS AND USES THEREOF - A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set). The use of such matched vector sets provides flexibility and versatility in the generation of eukaryotic display libraries, for example the ability to generate and to display multi-chain polypeptides by combining and recombining vectors that express variegations of the individual chains of a multi-chain polypeptide. Entire repertoires of novel chain combinations can be devised using such vector sets. | 11-17-2011 |
| 20130183758 | Host cells with artificial endosymbionts - The present invention is directed generally to eukaryotic host cells comprising artificial endosymbionts and methods of introducing artificial endosymbionts into eukaryotic host cells. The invention provides artificial endosymbionts that introduce a phenotype to host cells that is maintained in daughter cells. The invention additionally provides eukaryotic host cells containing magnetotactic bacteria. | 07-18-2013 |
| 20140308748 | NOVEL RECOMBINANT STRAIN OF TRICHODERMA USEFUL FOR ENHANCING NUTRITIONAL VALUE AND GROWTH OF PLANTS - The present invention relates to a novel recombinant fungal strain of | 10-16-2014 |
| 20150064787 | Host Cell Modification with Artificial Endosymbionts - The present invention is directed generally to host cells with artificial endosymbionts, wherein the artificial endosymbiont and the host cell communicate with each other to alter a phenotype of the host cell. In some embodiments, the communication comprises the secretion of a polypeptide from the artificial endosymbiont into the host cell. The secreted polypeptide can be a selectable marker, a reporter protein, a transcription factor, a signal pathway protein, a receptor, a growth factor, a cytokine, an effector molecule or other factors that can produce a phenotype in the host cell. | 03-05-2015 |
