| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 435441000 | Mutation employing a chemical mutagenic agent | 35 |
| 20090142837 | METHOD AND APPARATUS FOR SUBSTANTIALLY ISOLATING PLANT TISSUES - The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided. | 06-04-2009 |
| 20100055793 | SITE-SPECIFIC MODIFICATION OF THE HUMAN GENOME USING CUSTOM-DESIGNED ZINC FINGER NUCLEASES - Disclosed herein are chimeric zinc finger endonucleases useful in disrupting and/or replacing at least a portion of a gene of interest (e.g. CFTR, DMPK, CCR5, TYR or βglobin). | 03-04-2010 |
| 20100136693 | Mutants of Deoxycytidine Kinase Having Extended Enzymatic Activity - The invention relates to a method for artificial in vivo evolution of proteins, said method making it possible to bring about the evolution of a protein X by complementation of a relative protein Y, X and Y both belonging to the same class of enzyme commission (EC) nomenclature or belonging to related classes. The mutants D133E and R104Q of desoxycytidine kinase (DCK) were obtained; both of said mutations result in acquisition of thymidine kinase activity by DCK. | 06-03-2010 |
| 20100311169 | Methods Of Generating High-Production Of Antibodies From Hybridomas Created By In Vitro Immunization - The invention provides methods for generating high titers of high-affinity antibodies from hybridoma cells produced by fusing myeloma cells with in vitro immunized donor cells. The hybridoma cells or mammalian expression cells with cloned antibody genes from the hybridomas producing the high-affinity antibodies may be mismatch repair defective due to defects of endogenous mismatch repair subunits of through expression of a dominant negative allele of a mismatch repair gene which allows the hybridoma cell to be hypermutable, may be rendered hypermutable by chemical means, or may be naturally mismatch repair deficient. High-affinity antibodies and high titer producer cells producing antibodies may be prepared by the methods of the invention. | 12-09-2010 |
| 20110165679 | METHODS AND COMPOSITIONS FOR TARGETED POLYNUCLEOTIDE MODIFICATION - A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism. | 07-07-2011 |
| 20110201118 | NUCLEASE ACTIVITY OF TAL EFFECTOR AND FOKI FUSION PROTEIN - The present invention provides compositions and methods for targeted cleavage of cellular chromatin in a region of interest and/or homologous recombination at a predetermined site in cells. Compositions include fusion polypeptides comprising a TAL effector binding domain and a cleavage domain. The cleavage domain can be from any endonuclease. In certain embodiments, the endonuclease is a Type IIS restriction endonuclease. In further embodiments, the Type IIS restriction endonuclease is FokI. | 08-18-2011 |
| 20110212528 | Novel substitution mutant receptors and their use in an ecdysone receptor-based inducible gene expression system - This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms. | 09-01-2011 |
| 20110281359 | SPNK STRAINS - This invention includes spinosyn biosynthetic genes, spinosyn producing microorganisms transformed with the biosynthetic genes, methods using the biosynthetic genes to increase production of spinosyn insecticidal macrolides, and methods using the genes or fragments thereof to change the products produced by spinosyn-producing microorganisms. Additionally, the present invention includes methods and compositions for converting a spinosyn A and D producing strain to a spinetoram precursor, spinosyn J and L, producing strain. | 11-17-2011 |
| 20120021520 | RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 01-26-2012 |
| 20120021521 | RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 01-26-2012 |
| 20120040464 | METHOD FOR GENERATING HOMOPLASMIC CELLS FROM HETEROPLASMIC CELLS - It is an object of the present invention to provide a method, which comprises allowing a eukaryotic cell to come into contact with a reactive oxygen species or a chemical species that generates such a reactive oxygen species in the cell, and thereby changing the percentage of mutant mtDNA (mitochondrial genomic DNA) in the cell, and cells obtained by the above-described method. | 02-16-2012 |
| 20120083036 | POLYSACCHARIDE TRANSFERASE - The present invention is predicated on the discovery that a polysaccharide transferase purified from barley seedlings can catalyze the formation of covalent bonds between different polysaccharides, including between xyloglucans and cellulose, and between xyloglucans and (1,3;1,4)-β- | 04-05-2012 |
| 20120135524 | Novel Nucleic Acid Constructs Containing Orthogonal Site Selective Recombinases (OSSRs) - The present invention provides for a recombinant nucleic acid comprising a nucleotide sequence comprising a plurality of constructs, wherein each construct independently comprises a nucleotide sequence of interest flanked by a pair of recombinase recognition sequences. Each pair of recombinase recognition sequences is recognized by a distinct recombinase. Optionally, each construct can, independently, further comprise one or more genes encoding a recombinase capable of recognizing the pair of recombinase recognition sequences of the construct. The recombinase can be an orthogonal (non-cross reacting), site-selective recombinase (OSSR). | 05-31-2012 |
| 20120149115 | TARGETED GENOMIC REARRANGEMENTS USING SITE-SPECIFIC NUCLEASES - The present invention relates to a method for genomic DNA rearrangements, and more particularly, to a method for deletion, duplication, inversion, replacement, or rearrangement of genomic DNA using pairs of site-specific nucleases targeting two or more sites in the genome, a cell in which genomic DNA is deleted, duplicated, inverted, replaced, or rearranged by the same method, and a method for expressing the site-specific nucleases in cells. Further, the present invention relates to a method for inserting synthetic DNA molecules into the genome using site-specific nucleases targeting a pre-determined site in the genome, a cell in which DNA insertion occurs by the same method, and a method for expressing the site-specific nucleases in cells. | 06-14-2012 |
| 20120196370 | METHODS AND COMPOSITIONS FOR TARGETED GENOMIC DELETION - Disclosed herein are compositions and methods for generating chromosomal translocations and targeted deletions of specific lengths and at specific locations the genome of cell. | 08-02-2012 |
| 20120288940 | DIPEPTIDE ACETYLENE CONJUGATES AND A METHOD FOR PHOTOCLEAVAGE OF DOUBLE STRAND DNA BY DIPEPTIDE ACETYLENE CONJUGATES - Photoreactive DNA cleaving conjugate compounds are provided comprising a DNA cleaving moiety which comprises an aryl alkyne group and a polyfunctional pH-regulated DNA-binding moiety which comprises at least one or two amino groups. | 11-15-2012 |
| 20120288941 | USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF - Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and to its application for genome engineering and gene therapy. | 11-15-2012 |
| 20120288942 | USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF - Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and to its application for genome engineering and gene therapy. | 11-15-2012 |
| 20120288943 | USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF - Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and to its application for genome engineering and gene therapy. | 11-15-2012 |
| 20120301964 | METHODS AND COMPOSITIONS FOR GENETICALLY ENGINEERING CLOSTRIDIA SPECIES - The present invention relates to methods and compositions for engineering Clostridia species. In particular, embodiments of the present invention relate to the expression of recombinant resolvase proteins in Clostridia species. | 11-29-2012 |
| 20130023051 | TECHNIQUES FOR TRANSFECTING PROTOPLASTS - The invention relates to a method for the introduction of one or more molecules of interest in a plant cell protoplast by providing plant cell protoplasts, performing a first transfection of the plant cell protoplast with a composition that is capable of altering the regulation of one or more pathways selected from the group consisting of Mismatch Repair System and Non-Homologous End Joining and/or a composition that is capable of introducing DSBs, performing a second transfection of the plant cell protoplast with one or more molecules of interest such as mutagenic oligonucleotides and allowing the cell wall to form. | 01-24-2013 |
| 20130164850 | METHOD FOR TARGETED GENOMIC EVENTS IN ALGAE - The invention relates to endonucleases cleaving DNA target sequences from algae genomes, to appropriate vectors encoding such endonucleases, to cells or to algae modified by such vectors and to the use of these endonucleases and products derived therefrom for targeted genomic engineering in algae. | 06-27-2013 |
| 20130183757 | METHOD OF EFFICIENTLY ESTABLISHING INDUCED PLURIPOTENT STEM CELLS - Provided is a method of improving the efficiency of iPS cell establishment, comprising bringing one or more factors selected from the group consisting of proteins belonging to cyclin D family and nucleic acids that encode the same into contact with a somatic cell, in the step of nuclear reprogramming of the somatic cell. Also provided are a method of producing an iPS cell comprising the step of bringing the factor(s) and nuclear reprogramming substance(s) into contact with a somatic cell, an iPS cell comprising a nucleic acid that encodes a protein belonging to cyclin D family that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate. | 07-18-2013 |
| 20140004607 | TARGETED ALTERATION OF DNA | 01-02-2014 |
| 20140038291 | METHODS FOR REPROGRAMMING CELLS AND USES THEREOF - Described herein are reprogrammed cells, and methods for cell dedifferentiation, transformation and eukaryotic cell reprogramming. Also descried are cells, cell lines, and tissues that can be transplanted in a patient after steps of in vitro dedifferentiation and in vitro reprogramming. In particular embodiments the cells are Stem-Like Cells (SLCs), including Neural Stem-Like Cells (NSLCs), Cardiac Stem-Like Cells (CSLC), Hematopoietic Stem-Like Cells (HSLC), Pancreatic Progenitor-Like Cells, and Mesendoderm-like Cells. Also described are methods for generating these cells from human somatic cells and other types of cells. Also provided are compositions and methods of using of the cells so generated in human therapy and in other areas. | 02-06-2014 |
| 20140141516 | METHODS AND COMPOSITIONS FOR GENETICALLY ENGINEERING CLOSTRIDIA SPECIES - The present invention relates to methods and compositions for engineering | 05-22-2014 |
| 20140186957 | ENGINEERED TAL EFFECTOR PROTEINS WITH ENHANCED DNA TARGETING CAPACITY - The present invention provides compositions and methods for DNA targeting using TAL effectors and TAL effector based proteins, including but not limited to targeted gene regulation and targeted cleavage of cellular chromatin in a region of interest and/or homologous recombination at a predetermined site in cells. Compositions include fusion polypeptides comprising a TAL effector or a TAL effector binding domain in combination with other domains, including but not limited to a cleavage domain. The TAL effector binding domain includes modifications that increase activity of the same and also remove the constraints that the DNA target sequence be preceded by a thymine. | 07-03-2014 |
| 20140335618 | TAL EFFECTOR-MEDIATED DNA MODIFICATION - Materials and Methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; “TALENS”) are provided. | 11-13-2014 |
| 20140342456 | RNA-Guided Human Genome Engineering - A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner. | 11-20-2014 |
| 20140342457 | RNA-Guided Human Genome Engineering - A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner. | 11-20-2014 |
| 20140356956 | RNA-Guided Transcriptional Regulation - Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid. | 12-04-2014 |
| 20150353917 | METHODS AND COMPOSITIONS FOR NUCLEASE DESIGN - Methods and compositions for genetic alteration of cells are provided. | 12-10-2015 |
| 435442000 | By replacement of standard nucleic acid base with base analog (e.g., 5-bromouracil, etc.) | 1 |
| 20110312094 | USE OF DOUBLE STRANDED RNA TO INCREASE THE EFFICIENCY OF TARGETED GENE ALTERATION IN PLANT PROTOPLASTS - Method for targeted gene alteration in protoplasts of plant cells comprising the steps of transiently transfecting the protoplasts with a dsRNA that preferably targets plant MMR mRNA; and a mutagenic nucleobase. The transfection may be simultaneously or subsequently and the gene can be any gene functional in the mismatch repair system. | 12-22-2011 |
| 435444000 | By use of alkylating agent (e.g., nitrosoguanidine, etc.) | 1 |
| 20160002591 | Inactivation of Glutamyl Polypeptide Synthesis in Bacillus - The present invention relates to isolated polynucleotides of the chromosome of | 01-07-2016 |
| 435445000 | By use of oxidative deamination agent (e.g., nitrous acid, etc.) | 1 |
| 20080254539 | Mutated Xylanase Gene with High Reaction Activity and Site-Specific Mutagenesis Method Thereof - A mutated xylanase gene with high reaction activity includes a fifty-eighth amino acid or a thirty-eighth amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the thirty-eighth amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene. | 10-16-2008 |
