Entries |
Document | Title | Date |
20090004715 | Glycerol Feedstock Utilization for Oil-Based Fuel Manufacturing - The invention provides methods of manufacturing biodiesel and other oil-based compounds using glycerol and combinations of glycerol and other feedstocks as an energy source in fermentation of oil-bearing microorganisms. Methods disclosed herein include processes for manufacturing high nutrition edible oils from non-food feedstock materials such as waste products from industrial waste transesterification processes. Also included are methods of increasing oil yields by temporally separating glycerol and other feedstocks during cultivation processes. Also provided herein are oil-bearing microbes containing exogenous oil production genes and methods of cultivating such microbes on glycerol and other feedstocks. | 01-01-2009 |
20090017513 | PROCESS FOR PRODUCING HYDROCARBON MOLECULES FROM RENEWABLE BIOMASS - Provided is a process for producing hydrocarbon molecules from biomass utilizing microorganisms that are resistant to extreme heat and pressure, that comprise nucleic acid molecules encoding enzymes which degrade compounds selected from the group consisting of cellulose, lignin, xylan and hemicellulose, and which are capable of generating hydrocarbon molecules from the degraded cellulose, lignin, xylan and hemicellulose. | 01-15-2009 |
20090087889 | Methods and compositions for growth hydrocarbons in botryococcus sp. - Bottom dwelling colonies of | 04-02-2009 |
20090123984 | Transformed plants accumulating terpenes - The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT. | 05-14-2009 |
20090215140 | Novel Microalga and Process for Producing Hydrocarbon - An object of the present invention is to provide a novel microalga that produces hydrocarbons available as an alternative fuel to a diesel fuel (light oil). | 08-27-2009 |
20100041120 | Botryoccocus braunii Triterpene Synthase Proteins and Nucleic Acid Molecules, and Methods for Their Use - This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons. | 02-18-2010 |
20100120110 | CYTOCHROME P450S AND USES THEREOF - The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds. | 05-13-2010 |
20100120111 | METHOD OF PRODUCING HYDROCARBON BIOFUELS USING GENETICALLY MODIFIED SEAWEED - A method of producing hydrocarbon biofuels using genetically modified seaweed by inserting genes from high hydrocarbon producing micro algae into high growth seaweed species. In an embodiment of the present invention, | 05-13-2010 |
20100184177 | PLASTIC DISPOSABLE REACTOR SYSTEM - A plastic, disposable reactor (“PDR”) system is presented that will allow growth of microorganisms at various temperatures and pressures cost effectively. In this invention, the use of the system for aquaculture of algae is presented. The use of the reactor will allow carbon sequestration and significant production of a renewable energy source. The incorporation of recycled materials in various components of the plant also benefits the environment. | 07-22-2010 |
20100216203 | Algae bioreactor using submerged enclosures with semi-permeable membranes - Methods for producing hydrocarbons, including oil, by processing algae and/or other micro-organisms in an aquatic environment. Flexible bags (e.g., plastic) with CO | 08-26-2010 |
20100248321 | SURFACTANT AMENDMENTS FOR THE STIMULATION OF BIOGENIC GAS GENERATION IN DEPOSITS OF CARBONACEOUS MATERIALS - Methods of conditioning a carbonaceous material in a subterranean geologic formation for metabolism into a compound with enhanced hydrogen content by a microorganism consortium are described. The methods may include the steps of accessing the subterranean geologic formation through an access point, and contacting the carbonaceous material with a surfactant. The microorganism consortium can utilize the surfactant as a first nutrient source. The surfactant also increases accessibility of the carbonaceous material as a second nutrient source for the microorganism consortium. The microorganism consortium metabolizes the carbonaceous material into the compound with the enhanced hydrogen content. | 09-30-2010 |
20100267102 | WASTE DISTRIBUTION, CONVERSION, AND UTILIZATION - Food wastes streams may be managed efficiently by co-locating a waste-processing facility including a pulper and an anaerobic bioreactor with a food distribution facility. | 10-21-2010 |
20100304456 | Continuous-batch hybrid process for production of oil and other useful products from photosynthetic microbes - A process for cultivating photosynthetic microbes comprising Closed Systems for continuous cultivation and Open Systems for batch cultivation, in which (a) the Closed System Area occupies no more than 20% of the Total Land Area of the cultivation facility; (b) batch cultures in the Open Systems are initiated with an inoculum from the Closed Systems containing a cell biomass of no less than 5% of the carrying capacity of said Open System; (c) the doubling rate of said photosynthetic microbe is no less than once every 16 hours; and (d) the residence time of the batch culture in said Open System is no more than a period of 5 days. | 12-02-2010 |
20110008865 | INTEGRATED WASTE/HEAT RECYCLE SYSTEM - An integrated system and process for the treatment of organic fractions of municipal solid waste is described herein. The system and the process transform solid waste into fuel and energy. The integrated system and process comprise various different processes for pretreatment, sorting/separating, anaerobic digestion and conversion of biomass and gas to various gasseous, liquid and solid fuels and electricity. | 01-13-2011 |
20110091952 | METHOD FOR ENZYMATIC PRODUCTION OF DECARBOXYLATED POLYKETIDES AND FATTY ACIDS - Disclosed herein are methods of preparing alkenes from beta-hydroxy or beta-sulfate carboxylic acid or carboxylic acid derivatives using thioesterase and optionally a sulfotransferase. | 04-21-2011 |
20110111475 | Biological/Electrolytic Conversion of Biomass to Hydrocarbons - Hydrocarbon and hydrogen fuels and other products may be produced by a process employing a combination of fermentation and electrochemical stages. In the process, a biomass contained within a fermentation medium is fermented with an inoculum comprising a mixed culture of microorganisms derived the rumen contents of a rumen-containing animal. This inoculated medium is incubated under anaerobic conditions and for a sufficient time to produce volatile fatty acids. The resultant volatile fatty acids are then subjected to electrolysis under conditions effective to convert said volatile fatty acids to hydrocarbons and hydrogen simultaneously. The process can convert a wide range of biomass materials to a wide range of volatile fatty acid chain lengths and can convert these into a wide range of biobased fuels and biobased products. | 05-12-2011 |
20110269203 | Lipogenic zooplankton for the conversion of cellulosic biomass to biofuel - The present invention to provides a method for the conversion of biomass comprising cellulosic or carbohydrate polymers, into lipids useful for the production of biofuels. The method is achieved by providing the biomass to a culture of lipogenic zooplankton in a contained space, whereby the zooplankton consume the biomass, thereby converting the biomass to zooplankton derived biomass with increased lipid content. The zooplankton derived biomass is collected, lipids are extracted, and the lipids are converted to fuel through methods known in the art. | 11-03-2011 |
20120021475 | METHOD FOR PRODUCING (+)-ZIZAENE - A method of producing (+)-zizaene by contacting at least one polypeptide with farnesyl pyrophosphate (FPP) in vitro or in vivo to produce (+)-zizaene, a compound which can be used as precursor for diverse compounds useful in the fields of perfumery and flavoring. An amino acid sequence of a polypeptide useful in the method, a nucleic acid encoding the polypeptide of the invention, an expression vector containing the nucleic acid and a non-human host organism or a cell transformed to be used in the method of producing (+)-zizaene are also disclosed. | 01-26-2012 |
20120100588 | EFFICIENT OIL SHALE RECOVERY METHOD - A method for recovering hydrocarbons from oil shale and other carbonaceous materials has the steps of preparing a biomedium of microorganisms, water, and nutrients; providing shale laden with oil; treating oil shale with the biomedium; mechanically agitating the treated oil shale and biomedium approximately twelve hours to form a liquid suspension; and separating the components of the liquid suspension to yield a hydrocarbon mixture. | 04-26-2012 |
20120107893 | MICROBIAL ENGINEERING FOR THE PRODUCTION OF CHEMICAL AND PHARMACEUTICAL PRODUCTS FROM THE ISOPRENOID PATHWAY - The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids. | 05-03-2012 |
20120107894 | Methods and Compositions for the Recombinant Biosynthesis of Terminal Olefins - The present disclosure identifies methods and compositions for modifying microbial cells, such that the organisms efficiently synthesize terminal olefins, and in particular the use of such organisms for the commercial production of propylene and related molecules. | 05-03-2012 |
20120129235 | Novel fragrance and methods for production of 5-EPI- - vetivone,2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one, and 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-1,6-dien-8-one - The present invention is directed to novel methods for production of 5-epi-β-vetivone, 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one and 2-isopropyl-6,10-dimethylspiro[4.5]deca-1,6-dien-8-one, which are useful for their fragrant qualities. Provided are methods for production of premnaspirodiene from a terpene substrate, methods for production of 5-epi-β-vetivone from premnaspirodiene as starting material, and methods for production of 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one and 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-1,6-dien-8-one from premnaspirodiene as starting material. | 05-24-2012 |
20120171741 | Renewable Xylenes Produced from Bological C4 and C5 Molecules - The present invention is directed to a method for preparing renewable and relatively high purity p-xylene from biomass, and from C | 07-05-2012 |
20120184008 | Filamentous fungi and methods for producing trichodiene from lignocellulosic feedstocks - The present invention relates to the production of a C-15 fuel from lignocellulosic or other feedstock. Specifically at least double mutant of filamentous fungi having the isoprenoid pathway results in production of trichodiene in commercial quantities. One embodiment of the invention relates to producing the fuel at the site of the lignocellulosic feedstock to reduce costs of shipping the feedstock. | 07-19-2012 |
20120208253 | Methods and Compositions for Producing Alkenes of Various Chain Length - The NonA alkene synthase in | 08-16-2012 |
20120301940 | METHOD FOR PRETREATING CRUDE OIL USING MICROORGANISM - A method for pretreating a crude oil prior to a crude oil distillation process comprising the step of propagating a culture of a hydrocarbon-utilizing and biosurfactant-producing microbial strain and treating the crude oil with the propagated culture. | 11-29-2012 |
20120322126 | METHOD OF PRODUCING SYNTHETIC PETROLEUM FROM COAL OR HYDROCARBONS OR FROM C, H OR OXYGEN USING A HOST MICROORGANISM - The invention relates to a method of making microorganisms capable of producing petroleum from coal, or wood or certain other fossil fuels or raw materials, wherein gene sequences responsible for such production are isolated from microorganisms capable of such production, and transfected into suitable hosts, with better productivity or viability. The invention also includes using the same process to make elemental carbon, hydrogen and oxygen from organic or inorganic sources, including natural water or salt-water sources, petroleum, coal, other fossil fuel materials or other hydrocarbon sources, including turf, grass, glucose, rubber, sapropel, sapropelites, slates and wood; and it further includes making fossil fuels from water or from carbon, hydrogen and oxygen. In the alternative, the appropriate gene sequence can be used to make probes which can be used to find other gene sequences in other microorganisms which can optimize production. | 12-20-2012 |
20120322127 | METHOD OF PRODUCING SYNTHETIC PETROLEUM FROM COAL OR HYDROCARBONS OR FROM C,H OR OXYGEN USING A HOST MICROORGANISM - The invention relates to a method of making microorganisms capable of producing petroleum from coal, or wood or certain other fossil fuels or raw materials, wherein gene sequences responsible for such production are isolated from microorganisms capable of such production, and transfected into suitable hosts, with better productivity or viability. The invention also includes using the same process to make elemental carbon, hydrogen and oxygen from organic or inorganic sources, including natural water or salt-water sources, petroleum, coal, other fossil fuel materials or other hydrocarbon sources, including turf, grass, glucose, rubber, sapropel, sapropelites, slates and wood; and it further includes making fossil fuels from water or from carbon, hydrogen and oxygen. In the alternative, the appropriate gene sequence can be used to make probes which can be used to find other gene sequences in other microorganisms which can optimize production. | 12-20-2012 |
20120322128 | METHOD OF PRODUCING SYNTHETIC PETROLEUM FROM COAL OR HYDROCARBONS OR FROM C, H OR OXYGEN USING A HOST MICROORGANISM - The invention relates to a method of making microorganisms capable of producing petroleum from coal, or wood or certain other fossil fuels or raw materials, wherein gene sequences responsible for such production are isolated from microorganisms capable of such production, and transfected into suitable hosts, with better productivity or viability. The invention also includes using the same process to make elemental carbon, hydrogen and oxygen from organic or inorganic sources, including natural water or salt-water sources, petroleum, coal, other fossil fuel materials or other hydrocarbon sources, including turf, grass, glucose, rubber, sapropel, sapropelites, slates and wood; and it further includes making fossil fuels from water or from carbon, hydrogen and oxygen. In the alternative, the appropriate gene sequence can be used to make probes which can be used to find other gene sequences in other microorganisms which can optimize production. | 12-20-2012 |
20130005012 | ENZYMATIC SYSTEM FOR MONOMER SYNTHESIS - In vivo methods for production of styrene via a recombinant host cell can include cell expression of at least one polynucleotide encoding a polypeptide having cinnamic acid decarboxylase activity, and at least one polynucleotide encoding a polypeptide having phenylalanine ammonia lyase activity. | 01-03-2013 |
20130095544 | Pond System for Algae Growth and Harvesting - Management of a pond for algae growth and harvesting is facilitated by use of a cover system. The cover system can include one or more types of cover portions for management of various interactions between a pond and an ambient environment, such as management of incident light, thermal management, facilitating product recovery, and management of gas diffusion. The cover system can be used in conjunction with both active and passive mixing devices. | 04-18-2013 |
20130130344 | MICROORGANISM VARIANTS HAVING HYDROCARBON PRODUCING ABILITY AND METHOD FOR PRODUCING HYDROCARBON USING THE SAME - The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane. | 05-23-2013 |
20130130345 | PRODUCTION OF RENEWABLE AROMATIC COMPOUNDS - The invention provides a process for producing a variety renewable aromatic compounds such as benzene, toluene, xylenes, and cumene, as well as compounds derived from these including, for example, aniline, benzoic acid, cresol, cyclohexane, cyclohexanone, phenol and bisphenol A, toluene di-isocyanate, isophthalic acid, phthalic anhydride, terephthalic acid and dimethyl terephthalate. The invention also provides for renewable forms of these aromatic compounds. | 05-23-2013 |
20130143291 | PRODUCTION OF MONOTERPENES - The present invention relates to methods for producing monoterpenes, particularly tricyclene, by culturing microbial organisms that express a terpene synthase and optionally a prenyl transferase. | 06-06-2013 |
20130164806 | METHOD FOR PRODUCING RENEWABLE FUELS - According to the present invention, organic material is converted to biogas through anaerobic digestion and the biogas is purified to yield a combustible fluid feedstock comprising methane. A fuel production facility utilizes or arranges to utilize combustible fluid feedstock to generate renewable hydrogen that is used to hydrogenate crude oil derived hydrocarbons in a process to make liquid transportation or heating fuel. The renewable hydrogen is combined with crude oil derived hydrocarbons that have been desulfurized under conditions to hydrogenate the hydrocarbons with the renewable hydrogen. | 06-27-2013 |
20130164807 | METHOD FOR PRODUCING RENEWABLE FUELS - According to the present invention, organic material is converted to biogas through anaerobic digestion and the biogas is purified to yield a combustible fluid feedstock comprising methane. A fuel production facility utilizes or arranges to utilize combustible fluid feedstock to generate renewable hydrogen that is used to hydrogenate crude oil derived hydrocarbons in a process to make transportation or heating fuel. The renewable hydrogen is combined with crude oil derived hydrocarbons that have been desulfurized under conditions to hydrogenate the liquid hydrocarbon with the renewable hydrogen or alternatively, the renewable hydrogen can be added to a reactor operated so as to simultaneously desulfurize and hydrogenate the hydrocarbons. The present invention enables a party to receive a renewable fuel credit for the transportation or heating fuel. | 06-27-2013 |
20130203143 | Methods and Systems for the Production of Hydrocarbon Products - Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from a CO | 08-08-2013 |
20130224817 | Filamentous Fungi and Methods for Producing Isoprenoids - The present invention relates to the production of a isoprenoid products from a lignocellulosic feedstock. Specifically at least triple mutant of filamentous fungi having the isoprenoid pathway results in production of isoprenoid products in commercial quantities. One embodiment of the invention relates to producing the isoprenoid products at the site of the lignocellulosic feedstock to reduce costs of shipping the feedstock. | 08-29-2013 |
20130236942 | PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS - Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids. | 09-12-2013 |
20130236943 | Method for production of isoprenoid compounds - The present invention is directed to variant squalene synthase enzymes, including | 09-12-2013 |
20130309739 | METHODS AND SYSTEMS FOR PROCESSING BIOMASS MATERIAL - Embodiments of the present invention provide for efficient and economical production and recovery of volatile organic compounds and hydrocarbons. One embodiment comprises contacting a solid component of a biomass material with a solution adapted to facilitate saccharification, and contacting the at least one fermentable sugar with a microorganism capable of using the at least one fermentable sugar to generate a hydrocarbon. The solid component is generated by introducing a biomass material to a compartment of a solventless recovery system, wherein the biomass material contains one or more volatile organic compounds; contacting the biomass material with a superheated vapor stream in the compartment to vaporize at least a portion of an initial liquid content in the biomass material; separating a vapor component and a solid component from the heated biomass material; and retaining at least a portion of the gas component for use as part of the superheated vapor stream. | 11-21-2013 |
20130330795 | METHODS FOR PREPARING A HYDROCARBON - Method for preparing a mono-unsaturated alkene comprising contacting an aliphatic mono-unsaturated carboxylic acid with an Fdc1 polypeptide comprising an amino acid sequence with at least 21% sequence identity to SEQ ID NO: 1 and a Pad1 polypeptide comprising an amino acid sequence with at least 17% sequence identity to SEQ ID NO: 2. | 12-12-2013 |
20140017751 | METHOD FOR PRODUCING ORGANIC COMPOUNDS VIA FERMENTATION OF BIOMASS AND ZEOLITE CATALYSIS - The invention relates to a method for obtaining organic compounds from biomass, wherein the steps of gas stripping, adsorption from the gas phase, and catalytic reaction are coordinated with each other. The method according to the invention preferably comprises the steps of fermentation, gas stripping, adsorption, desorption, catalytic reaction, condensation, and decantation, which can proceed in parallel. The invention further relates to the coupling of adsorption, desorption, and catalytic reaction by using the same zeolite material for adsorption and catalytic reaction. | 01-16-2014 |
20140030784 | Terpene Synthases and Methods of Using the Same - Disclosed are isolated nucleic acid molecules from | 01-30-2014 |
20140045238 | Method for producing terpenes - The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene cluster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use of | 02-13-2014 |
20140045239 | RENEWABLE ENGINE FUEL AND METHOD OF PRODUCING SAME - The present invention provides high-octane fuel, and a method of producing same. These fuels may be formulated to have a wide range of octane values and energy, and may effectively be used to replace 100 LL aviation fuel (known as AvGas), as well as high-octane, rocket, diesel, turbine engine fuels, as well as two-cycle, spark-ignited engine fuels. | 02-13-2014 |
20140099691 | PROCESS FOR PRODUCING LIQUID HYDROCARBON - The invention relates to a continuous process for converting carbonaceous material contained in one or more feedstocks into a liquid hydrocarbon product, said feedstocks including the carbonaceous material being in a feed mixture including one or more fluids, said fluids including water and further liquid organic compounds at least partly produced by the process in a concentration of at least 1% by weight, where the process comprises converting at least part of the carbonaceous material by pressurising the feed mixture to a pressure in the range 50-400 bar, heating the feed mixture to a temperature in the range 250-500° C., and maintaining said pressurized and heated feed mixture in the desired pressure and temperature ranges in a reaction zone for a predefined time; cooling the feed mixture to a temperature in the range 25-200° C. and expanding the feed mixture to a pressure in the range of 1-70 bar, thereby causing the carbonaceous material to be converted to a liquid hydrocarbon product; separating a fraction comprising liquid hydrocarbon product, and leaving a residual fraction; feeding said residual fraction into a bioreactor for the production of biomass such as algae and/or bacteria such as cyano bacteria. | 04-10-2014 |
20140113343 | MODIFIED MICROORGANISMS AND USE THEREOF FOR TERPENE PRODUCTION - The present invention relates to the control of gene expression by a heterologous glucose-regulated promoter, to microorganisms in which gene expression is controlled by a heterologous glucose-regulated promoter and to methods using said microorganisms for the production of terpenes during glucose-limited fed-batch fermentation. | 04-24-2014 |
20140134694 | SYSTEMS AND METHODS FOR PROCESSING MIXED SOLID WASTE - Solid waste that includes a mixture of wet organic material and dry organic material can be are separated using mechanical separation to produce a wet organic stream enriched in wet organics and a dry organic stream enriched in dry organics. The separated wet organic stream and dry organic stream are separately converted to renewable or recyclable products using different conversion techniques particularly suited for the separated wet and dry organic streams. | 05-15-2014 |
20140154765 | PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS - Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids. | 06-05-2014 |
20140154766 | Method of Producing Coupled Radical Products from Biomass - A method that produces coupled radical products from biomass. The method involves obtaining a lipid or carboxylic acid material from the biomass. This material may be a carboxylic acid, an ester of a carboxylic acid, a triglyceride of a carboxylic acid, or a metal salt of a carboxylic acid, or any other fatty acid derivative. This lipid material or carboxylic acid material is converted into an alkali metal salt. The alkali metal salt is then used in an anolyte as part of an electrolytic cell. The electrolytic cell may include an alkali ion conducting membrane (such as a NaSICON membrane). When the cell is operated, the alkali metal salt of the carboxylic acid decarboxylates and forms radicals. Such radicals are then bonded to other radicals, thereby producing a coupled radical product such as a hydrocarbon. The produced hydrocarbon may be, for example, saturated, unsaturated, branched, or unbranched, depending upon the starting material. | 06-05-2014 |
20140162336 | MICROORGANISM MEDIATED LIQUID FUELS - Herein disclosed is a method for producing liquid hydrocarbon product, the method comprising disintegrating a hydrocarbon source; pretreating the disintegrated hydrocarbon source; solubilizing the disintegrated hydrocarbon source to form a slurry comprising a reactant molecule of the hydrocarbon source; admixing a biochemical liquor into the slurry, wherein the biochemical liquor comprises at least one conversion enzyme configured to facilitate bond selective photo-fragmentation of said reactant molecule of the hydrocarbon source, to form liquid hydrocarbons via enzyme assisted bond selective photo-fragmentation, wherein said conversion enzyme comprises reactive sites configured to restrict said reactant molecule such that photo-fragmentation favorably targets a preselected internal bond of said reactant molecule; separating the liquid hydrocarbons from the slurry, wherein contaminants remain in the slurry; and enriching the liquid hydrocarbons to form a liquid hydrocarbon product. Various aspects of such method/process are also discussed. | 06-12-2014 |
20140242658 | Method for production of isoprenoid compounds - The present invention is directed to variant squalene synthase enzymes, including | 08-28-2014 |
20140242659 | METHOD FOR PRODUCING RENEWABLE FUELS - According to the present invention, organic material is converted to biogas through anaerobic digestion and the biogas is purified to yield a combustible fluid feedstock comprising methane. A fuel production facility utilizes or arranges to utilize combustible fluid feedstock to generate renewable hydrogen that is used to hydrogenate crude oil derived hydrocarbons in a process to make transportation or heating fuel. The renewable hydrogen is combined with crude oil derived hydrocarbons that have been desulfurized under conditions to hydrogenate the liquid hydrocarbon with the renewable hydrogen. The present invention enables a party to receive a renewable fuel credit for the transportation or heating fuel. | 08-28-2014 |
20140295517 | Host Cells and Constructs Useful for Producing Pinene - The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker. | 10-02-2014 |
20140349361 | Process for Converting Biomass to Aromatic Hydrocarbons - The present invention provides methods, reactor systems, and catalysts for increasing the yield of aromatic hydrocarbons produced while converting biomass to hydrocarbons. The invention includes methods of using catalysts to increase the yield of benzene, toluene, and mixed xylenes in the hydrocarbon product. | 11-27-2014 |
20140356926 | Novel sesquiterpene synthase gene and protein - The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention. | 12-04-2014 |
20140370562 | PRODUCTION OF BETA-PHELLANDRENE USING GENETICALLY ENGINEERED CYANOBACTERIA - The present invention provides methods and compositions for producing β-phellandrene hydrocarbons from cyanobacteria. | 12-18-2014 |
20150087042 | Genetically Modified Host Cells and Use of Same for Producing Isoprenoid Compounds - The present invention provides genetically modified eukaryotic host cells that produce isoprenoid precursors or isoprenoid compounds. A subject genetically modified host cell comprises increased activity levels of one or more of mevalonate pathway enzymes, increased levels of prenyltransferase activity, and decreased levels of squalene synthase activity. Methods are provided for the production of an isoprenoid compound or an isoprenoid precursor in a subject genetically modified eukaryotic host cell. The methods generally involve culturing a subject genetically modified host cell under conditions that promote production of high levels of an isoprenoid or isoprenoid precursor compound. | 03-26-2015 |
20150337324 | METHOD FOR REGULATING EXPRESSION OF SPECIFIC PROTEIN USING CYTOKININ-RESPONSIVE TRANSCRIPTION FACTOR, ISOPRENOID-PRODUCING PLANT HAVING GENE ENCODING CYTOKININ-RESPONSIVE TRANSCRIPTION FACTOR INTRODUCED THEREIN, AND METHOD FOR PRODUCING POLYISOPRENOID USING SAID ISOPRENOID-PRODUCING PLANT - The present invention provides a method for enhancing the overall pathway of polyisoprenoid biosynthesis. The present invention further provides an isoprenoid-producing plant having an overall enhanced pathway of polyisoprenoid biosynthesis, and a method of producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to a method of regulating by a cytokinin-responsive transcription factor the expression of at least one protein selected from the group consisting of hydroxymethylglutaryl-CoA reductase, isopentenyl diphosphate isomerase, cis-prenyltransferase, and small rubber particle protein. | 11-26-2015 |
20150337336 | ENZYMES AND METHODS FOR STYRENE SYNTHESIS - The subject technology generally relates to biosynthesis of styrene. Certain embodiments of the subject technology is based, in part, on the recognition that phenylalanine can be converted to styrene by a two-step pathway of deamination and decarboxylation, with trans-cinnamic acid (tCA) as the intermediate. Two types of enzymes are directly involved in this process, phenylalanine ammonia lyase (PAL), which converts phenylalanine to tCA, and cinnamic acid decarboxylase, which coverts tCA to styrene. Host cells expressing these two types of enzymes can be cultured in bioreactor to produce styrene from renewable substrates such as glucose. | 11-26-2015 |
20160153007 | METHOD OF INCREASING PRODUCTION OF AMORPHA-4,11-DIENE AND METHOD OF INCREASING PRODUCTION OF NATURAL RUBBER | 06-02-2016 |
20160168595 | TWO-PHASE FERMENTATION PROCESS FOR THE PRODUCTION OF AN ORGANIC COMPOUND | 06-16-2016 |
20160186161 | Alkenol dehydratase variants - Described are alkenol dehydratase variants having improved activity in catalyzing the conversion of a compound corresponding to the general formula C | 06-30-2016 |