Entries |
Document | Title | Date |
20080241900 | HIGHLY ACTIVE XYLOSE REDUCTASE FROM NEUROSPORA CRASSA - A new xylose reductase encoding gene from Neurspora crassa was heterologously expressed in | 10-02-2008 |
20090029431 | PROCESS FOR THE PREPARATION OF 1,3-PROPANEDIOL BY A RECOMBINANT MICRO-ORGANISM IN THE ABSENCE OF COENZYME B12 OR ONE OF ITS PRECURSORS - The invention concerns a method for preparing 1,3-propanediol from a carbon-containing substance, said method comprising a step which consists in culturing a recombinant micro-organism not producing coenzyme B12 in the absence of coenzyme B12 or one of its precursors. The invention also concerns a nucleic acid coding for a glycerol dehydratase whereof the catalytic activity is independent of the presence of coenzyme B12 or one of its precursors and a nucleic acid coding for a 1,3-propanol dehydrogenase intervening in the synthesis of 1,3-propanediol. The invention further concerns recombinant vectors and host cells comprising said nucleic acids and the polypeptides coded by the latter. | 01-29-2009 |
20090047719 | Methods and organisms for the growth-coupled production of 1,4-butanediol - The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby theone or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO. | 02-19-2009 |
20090061494 | Enzymatic Conversion of Epoxides to Diols - Diols of the formula I: or corresponding polymers wherein R | 03-05-2009 |
20090081745 | METHOD FOR PRODUCING 1,3-PROPANEDIOL AND 2,3-BUTANEDIOL FROM RAW STARCH MATERIAL - The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g/L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when the concentration of glycerin is reduced to a level below 10 g/L, and finally 1,3-propanediol and 2,3-butanediol are obtained. The method of the present invention can effectively reduce production cost and increase productivity. | 03-26-2009 |
20090197313 | METHOD FOR PREPARING XYLITOL WITH HIGH YIELD USING RECYCLING MICROORGANISM - Provided is a process for continuously producing xylitol in high yield and productivity using a vacuum microfiltration bioreactor containing a fermentation medium for a strain of the genus | 08-06-2009 |
20090246843 | PROCESS FOR PRODUCING ERYTHRITOL USING MONILIELLA TOMENTOSA STRAINS IN THE PRESENCE OF NEUTRAL INORGANIC NITRATES, SUCH AS POTASSIUM NITRATE, AMMONIUM NITRATE OR SODIUM NITRATE, AS NITROGEN SOURCE - The present invention relates to the use of at least one inorganic nitrate in a fermentation process for producing erythritol using a yeast strain of the | 10-01-2009 |
20100028965 | Method for producing 1,3-propanediol using crude glycerol, a by-product from biodiesel production - The invention discloses a method for producing 1,3-propanediol, comprising the steps of: using crude glycerol, a by-product during the biodiesel production, without further treatment, as the substrate for production of 1,3-propanediol; inoculating a 1,3-propanediol-producing strain in a seed medium containing crude glycerol, a by-product from biodiesel production; adding the seed culture into a fermentation medium containing crude glycerol, a by-product from biodiesel production, and fermenting; maintaining pH in a range of 6.8 to 8.0; and in the end of the fermentation, isolating and purifying 1,3-propanediol. | 02-04-2010 |
20100081181 | METHOD FOR CONVERSION OF OIL-CONTAINING ALGAE TO 1,3-PROPANEDIOL - The present invention relates to a process for oxidizing renewable polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to a malonic acid intermediate which is subsequently reduced to 1,3 propanediol (PDO). | 04-01-2010 |
20100112654 | MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL - The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO. | 05-06-2010 |
20100196977 | METHODS OF PRODUCING ISOPRENE AND A CO-PRODUCT - The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. | 08-05-2010 |
20100261239 | METABOLICALLY ENGINEERED MICROORGANISM USEFUL FOR THE PRODUCTION OF 1,2-PROPANEDIOL - Microorganism useful for the production of 1,2-propanediol from a carbon source, wherein said microorganism is characterized by:
| 10-14-2010 |
20100285547 | NEW MICRO-ORGANISMS FOR THE PRODUCTION OF 1,2-PROPANEDIOL OBTAINED BY A COMBINATION OF EVOLUTION AND RATIONAL DESIGN - The present invention concerns a new method combining evolution and rational design for the preparation of a strain of micro-organism for the production of 1,2-propanediol from a carbon source. | 11-11-2010 |
20100285548 | Process And Apparatus For The Microbial Production Of A Specific Product And Methane - The process according to the invention for the microbial production of a specific product and methane comprises the following steps: a) the production in a bioreactor ( | 11-11-2010 |
20100291645 | XYLOSE REDUCTASE MUTANTS AND USES THEREOF - Engineered mutant xylose reductases demonstrate higher preference to xylose than arabinose. Amino acid mutations were engineered in to native xylose reductase from | 11-18-2010 |
20100330634 | Mutants Having Capability To Produce 1, 4-Butanediol And Method For Preparing 1, 4-Butanediol Using The Same - A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process. | 12-30-2010 |
20100330635 | ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL - A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO. | 12-30-2010 |
20110003355 | PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH - A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO. | 01-06-2011 |
20110003356 | PROCESS FOR PRODUCTION OF XYLITOL - The invention provides a process for producing xylitol from xylose by a yeast strain capable of converting the xylose to xylitol comprising independently growing the yeast strain in a medium; transferring the yeast strain from the medium to a feed solution, the feed solution comprising of xylose in water, and separating the xylitol from said feed solution. | 01-06-2011 |
20110014669 | Production of 1,4 Butanediol in a Microorganism - A biological method for the conversion of L-glutamate to 1,4-butanediol that involves a decarboxylation step and avoids production of 4-hydroxybutyrate as an intermediate is described. The method includes: (a) conversion of L-glutamate to L-glutamate 5-phosphate; (b) conversion of L-glutamate 5-phosphate to L-glutamate 5-semialdehyde; (c) conversion of L-glutamate 5-semialdehyde to 5-hydroxy-L-norvaline; (d) conversion of 5-hydroxy-L-norvaline to 5-hydroxy-2-oxopentanoate; (e) conversion of 5-hydroxy-2-oxopentanoate to 4-hydroxybutanal; and (f) the conversion of 4-hydroxybutanal to 1,4-butanediol. | 01-20-2011 |
20110091949 | Process for Producing D-Mannitol - High concentration of free cells of heterofermentative lactic acid bacteria (LAB) in a resting or slowly growing state are used to convert fructose into mannitol. Efficient volumetric mannitol productivities and mannitol yields from fructose are achieved in a process applying cell-recycle, continuous stirred tank reactor and/or circulation techniques with native LAB cells or with LAB cells with inactivated fructokinase gene(s). Mannitol is recovered in high yield and purity with the aid of evaporation and cooling crystallization. | 04-21-2011 |
20110097772 | METHOD FOR PRODUCING XYLITOL FROM LIGNOCELLULOSIC HYDROLYSATES WITHOUT DETOXIFICATION - A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast | 04-28-2011 |
20110104772 | Method of Cultivating Yeast for Enhancing Pentitol Production - A method for culturing the yeast for enhancing pentitol production is provided. The yeast cultured according to the present disclosure is | 05-05-2011 |
20110124069 | PRODUCTION METHOD - The invention relates to the development of microorganisms that produce 1,2-propanediol (1,2-PD) from glycerol, whereas glycerol is simultaneously the substrate carbon source for 1,2-PD- and biomass production. The invention demonstrates that any type of glycerol serves as carbon substrate for 1,2-PD biosynthesis. The microorganism is a recombinant organism, preferentially an | 05-26-2011 |
20110201071 | Methods and organisms for the growth-coupled production of 1,4-Butanediol - The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby theone or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO. | 08-18-2011 |
20110207189 | METHODS FOR INCREASING PRODUCT YIELDS - A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product. | 08-25-2011 |
20110207190 | METHODS OF XYLITOL PREPARATION - The invention provides spray-dried preparations of microbes useful for oxidoreductase reactions, e.g., xylitol production, and methods of making and using those microbes. | 08-25-2011 |
20110217744 | USE OF SUCROSE AS SUBSTRATE FOR FERMENTATIVE PRODUCTION OF 1,2-PROPANEDIOL - The present invention is relative to a method for producing 1,2-propanediol by fermentation, comprising: cultivating a microorganism producing 1,2-propanediol in an appropriate medium comprising a source of sucrose, and recovering the 1,2-propanediol being produced, wherein the microorganism is able to utilize sucrose as sole carbon source for the production of 1,2-propanediol. In a preferred aspect of the invention, the source of sucrose is obtained from plant biomass, and is in particular sugar cane juice. | 09-08-2011 |
20110294178 | METHOD FOR THE PREPARATION OF DIOLS - The present invention concerns a new method for the biological preparation of a diol comprising culturing a microorganism genetically modified for the bioproduction of an aliphatic diol, wherein the microorganism comprises a metabolic pathway for the decarboxylation of a hydroxy-2-keto-aliphatic acid metabolite with an enzyme having a 2-keto acid decarboxylase activity, the product obtained from said decarboxylation step being further reduced into the corresponding aliphatic diol, and wherein the microorganism is genetically modified for the improved production of said hydroxy-2-keto-aliphatic acid metabolite. | 12-01-2011 |
20110312049 | MICROORGANISMS AND METHODS FOR THE PRODUCTION OF ETHYLENE GLYCOL - The invention provides non-naturally occurring microbial organisms having an ethylene glycol pathway. The invention additionally provides methods of using such organisms to produce ethylene glycol. | 12-22-2011 |
20110312050 | CONTINUOUS CATALYTIC GENERATION OF POLYOLS FROM CELLULOSE - A catalytic process for generating at least one polyol from a feedstock comprising cellulose is performed in a continuous manner using a catalyst comprising nickel tungsten carbide. The process involves, contacting, continuously, hydrogen, water, and a feedstock comprising cellulose, with the catalyst to generate an effluent stream comprising at least one polyol and recovering the polyol from the effluent stream. | 12-22-2011 |
20110312051 | PROCESS FOR GENERATION OF POLYOLS FROM SACCHARIDE CONTAINING FEEDSTOCK - A process for generating at least one polyol from a feedstock comprising saccharide is performed in a continuous or batch manner. The process involves, contacting hydrogen, water, and a feedstock comprising saccharide, with a catalyst system to generate an effluent stream comprising at least one polyol and recovering the polyol from the effluent stream. The catalyst system comprises at least one metal component with an oxidation state greater than or equal to 2+. | 12-22-2011 |
20120045808 | PRODUCTION METHOD FOR 1,3-PROPANEDIOL USING RECOMBINANT MICROBIAL STRAIN HAVING GLYCEROL OXIDATION METABOLIC PATHWAY BLOCKED - Provided is a method of producing 1,3-propanediol by culturing a recombinant strain in which the glycerol oxidative pathway had been blocked, and more particularly a method of producing 1,3-propanediol by two-step culture of a recombinant strain in which the oxidative pathway that produces byproducts in the glycerol metabolic pathway had been blocked. When the recombinant strain in which the glycerol oxidative pathway that produces byproducts had been blocked is cultured in two steps, 1,3-propanediol can be produced with improved yield without producing products that result in an increase in purification costs. | 02-23-2012 |
20120058531 | CONTINUOUS CULTURE FOR 1,3-PROPANEDIOL PRODUCTION USING HIGH GLYCERINE CONCENTRATION - The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a microorganism on a culture medium with high glycerine content. The invention also concerns a new microorganism, or strain of microorganism, adapted for the production of 1,3-propanediol from medium comprising high glycerine content. | 03-08-2012 |
20120094345 | COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS - The invention provides a non-naturally occurring microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (1,4-BDO) biosynthetic pathways. The pathways include exogenous nucleic acids encoding a) an α-ketoglutarate decarboxylase; b) a 4-hydroxybutanoate dehydrogenase; c) a 4-hydroxybutyryl-CoA:acetyl-CoA transferase or a butyrate kinase and a phosphotransbutyrylase; d) an aldehyde dehydrogenase, and e) an alcohol dehydrogenase, wherein the exogenous nucleic acids are expressed in sufficient amounts to produce 1,4-butanediol (1,4-BDO). Also provide is a method for the production of 1,4-BDO. The method includes culturing the non-naturally occurring microbial organism having 4-HB and 1,4-BDO biosynthetic pathways substantially anaerobic conditions for a sufficient period of time to produce 1,4-BDO. | 04-19-2012 |
20120107889 | ENDOPHYTIC YEAST STRAINS, METHODS FOR ETHANOL AND XYLITOL PRODUCTION, METHODS FOR BIOLOGICAL NITROGEN FIXATION, AND A GENETIC SOURCE FOR IMPROVEMENT OF INDUSTRIAL STRAINS - The present invention provides novel endophytic yeast strains capable of metabolizing both pentose and hexose sugars. Methods of producing ethanol and xylitol using the novel endophytic yeast are provided herein. Also provided are methods of fixing nitrogen and fertilizing a crop using the novel endophytic yeast strains provided herein. | 05-03-2012 |
20120122171 | COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS - The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method are methods for the production of 4-HB and BDO. | 05-17-2012 |
20120135487 | MUTANT GLYCEROL DEHYDROGENASE (GLYDH) FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION - The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a microorganism modified for an improved production of 1,2-propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced. | 05-31-2012 |
20120164704 | SUGAR TRANSPORT SEQUENCES, YEAST STRAINS HAVING IMPROVED SUGAR UPTAKE, AND METHODS OF USE - Disclosed are nucleic acid constructs comprising coding sequences operably linked to a promoter not natively associated with the coding sequence. The coding sequences encode | 06-28-2012 |
20120171739 | Xylitol Producing Microorganism Introduced with Arabinose Metabolic Pathway and Production Method of Xylitol Using the Same - The present invention relates to an efficient production method of xylitol by using the xylitol producing microorganism introduced with arabinose metabolic pathway to inhibit the production of arabitol, the byproduct, and instead to use arabinose only for cell metabolism in xylose/arabinose mixed medium. More precisely, to express efficiently L-arabinose isomerase (araA), L-ribulokinase (araB) and L-ribulose-5-phosphate 4-epimerase (araD) in | 07-05-2012 |
20120208249 | MICROORGANISMS AND METHODS FOR CONVERSION OF SYNGAS AND OTHER CARBON SOURCES TO USEFUL PRODUCTS - A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. | 08-16-2012 |
20120225463 | MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL AND RELATED METHODS - The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO. | 09-06-2012 |
20120231514 | FERMENTATION METHOD TO PRODUCE A LIGNOCELLULOSE-BASED SUGAR STREAM WITH ENRICHED PENTOSE CONTENT - A method for producing a fermented solution enriched in xylose is provided. The sugar stream that is fermented results from the hydrolysis of a lignocellulosic feedstock and comprises at least a hexose and a pentose. The method comprises fermenting the sugar stream resulting from the hydrolysis of the lignocellulosic feedstock with a microorganism that preferentially ferments the hexose over the pentose. The fermenting is conducted under aerobic conditions and comprises continuously feeding the sugar stream to a fermentation reactor at a dilution rate that converts the hexose to cell mass preferentially over the pentose, thereby reducing the concentration of the glucose in the sugar stream so that the fermented solution thus produced is enriched in the pentose relative to said sugar stream. | 09-13-2012 |
20120276606 | RECOMBINANT MICROORGANISMS WITH 1,3-BUTANEDIOL-PRODUCING FUNCTION AND USES THEREOF - An objective of the present invention is to provide recombinant microorganisms efficiently producing optically active 1,3-butanediol, which is useful as a material for synthesizing pharmaceuticals and such, and provide methods for efficiently producing optically active 1,3-butanediol using the recombinant microorganisms. As a result of dedicated research for achieving the above objective, the present inventors succeeded in producing recombinant microorganisms in which the activity of an enzyme catalyzing the reduction reaction represented by Formula 1 is enhanced and which produce a diol compound represented by Formula 2. | 11-01-2012 |
20120329113 | Microorganisms for Producing 1,3-Butanediol and Methods Related Thereto - Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO. | 12-27-2012 |
20130017587 | Method for Recovery of Organic Components from Dilute Aqueous SolutionsAANM Nierlich; FranzAACI MarlAACO DEAAGP Nierlich; Franz Marl DEAANM Burger-Kley; WalterAACI KlagenfurtAACO ATAAGP Burger-Kley; Walter Klagenfurt ATAANM Mikenberg; IljaAACI BochumAACO DEAAGP Mikenberg; Ilja Bochum DEAANM Kneissel; BernhardAACI BonnAACO DEAAGP Kneissel; Bernhard Bonn DE - The present invention relates to a method for recovering an organic component from an aqueous medium such as a fermentation broth containing microorganism producing said organic component. The method includes increasing the activity of the organic component in the aqueous medium by increasing the concentration of at least one hydrophilic solute in the medium leading to salting-out of the organic component. The microorganisms are genetically modified to be capable of tolerating higher concentrations in the medium in comparison to their unmodified counterparts. | 01-17-2013 |
20130034889 | NOVEL MICROORGANISM, AND HYDROGEN PRODUCTION PROCESS, 1,3-PROPANEDIOL PRODUCTION PROCESS AND BIODIESEL LIQUID WASTE TREATMENT METHOD EACH USING THE MICROORGANISM - The present invention provides a microorganism belonging to the genus | 02-07-2013 |
20130052707 | METHOD AND APPARATUS FOR ACCELERATING BIOTECHNICAL REACTION AND PRODUCTION - The method and apparatus according to the present invention concentrate the formation of the biotechnical product to active site in the reactor, where the production is accelerated to extreme speeds by altering the conditions. To achieve this, anabolic, catabolic or overflow metabolic reactions can be utilised. The aim is to implement production so that carbon dioxide emissions in particular are minimised The products are e.g. 2,3-butanediol, butanol, ethanol, acetone, organic acids, methane or hydrogen gas and other fuels or compounds necessary for chemical or material production. | 02-28-2013 |
20130065288 | PRODUCTION OF XYLITOL FROM A MIXTURE OF HEMICELLULOSIC SUGARS - Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams. | 03-14-2013 |
20130109069 | MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL | 05-02-2013 |
20130177955 | PROCESS FOR PRODUCTION OF ALCOHOLS BY MICROBIAL FERMENTATION - The invention provides method for producing butanediol and specifically 2,3-butanediol by anaerobic fermentation of a gaseous substrate comprising CO by a culture of at least one micro-organism. In accordance with particular methods of the invention, 2,3-butanediol is produced by anaerobic fermentation of substrates including carbohydrates and carbon monoxide. The invention further provides for the up-regulation of a native 2,3-butanediol dehydrogenase gene in the at least one micro-organism. The 2,3-butanediol can be further converted to compounds such as butene(s), butadiene and methyl ethyl ketone. These compounds can be further converted to chemical products such as 2-butanol. | 07-11-2013 |
20130177956 | MICROORGANISMS FOR 1,3-PROPANEDIOL PRODUCTION USING HIGH GLYCERINE CONCENTRATION - The present invention is related to a population of | 07-11-2013 |
20130183729 | METHOD FOR PRODUCING XYLITOL FROM LIGNOCELLULOSIC HYDROLYSATES WITHOUT DETOXIFICATION - A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast | 07-18-2013 |
20130189752 | ENERGY EFFICIENT METHODS TO PRODUCE PRODUCTS - The invention relates to processes that efficiently convert carbon-containing materials, such as biomass, into products in such a manner that the energy, carbon, and mass content of the materials are efficiently transferred into such products. Such methods include converting the materials into at least one intermediate by a biological conversion process and at least one intermediate by a thermochemical conversion process and reacting the intermediates to form the product. Such methods have a chemical energy efficiency to produce the product that is greater than the chemical energy efficiency of a solely biological conversion process to produce the product and that is greater than the chemical energy efficiency of a process in which all of the material is initially subjected to a thermochemical conversion step as part of the process to produce the product. | 07-25-2013 |
20130203141 | PRODUCTION OF 1,4-BUTANEDIOL BY RECOMBINANT MICROORGANISMS - Provided herein are metabolically-modified microorganisms useful for producing 1,4-butanediol. | 08-08-2013 |
20130210099 | METHOD FOR THE PRODUCTION OF ERYTHRITOL - The invention relates to a method for the production of erythritol, in which erythrose is reduced to erythritol using a NADPH-specific enzyme, wherein the enzyme is an erythrose reductase which was isolated from the group of saprophytes selected from the strains of | 08-15-2013 |
20130230894 | Optimised Fermentation Media - The invention relates to improvements in the production of alcohols by microbial fermentation, particularly to production of alcohols by microbial fermentation of a substrate comprising CO. It more particularly relates to the addition of an inorganic sulfur source to a fermentation system such that one or more micro-organisms convert a substrate comprising CO to alcohols. In a particular embodiment, a microbial culture is provided with sodium polysulfide, wherein a substrate comprising CO is converted to products including ethanol and 2,3-butanediol. | 09-05-2013 |
20130309737 | METHOD FOR THE PREPARATION OF 1,3-PROPANEDIOL FROM SUCROSE - The present invention concerns a microorganism genetically modified for the bioproduction of 1,3-propanediol from sucrose, wherein the microorganism comprises:
| 11-21-2013 |
20130316416 | ENGINEERING MICROBES AND METABOLIC PATHWAYS FOR THE PRODUCTION OF ETHYLENE GLYCOL - The invention relates to recombinant cells and their use in the production of ethylene glycol. | 11-28-2013 |
20130316417 | PROCESS FOR THE CONVERSION OF GLYCEROL TO 1,3-PROPANEDIOL - The present application includes a process and a microorganism of the genus | 11-28-2013 |
20130316418 | METHOD FOR PRODUCING 2,3-BUTANEDIOL BY FERMENTATION - The invention relates to a production strain for producing 2,3-butanediol. Said production strain can be produced from an original strain selected from the genes | 11-28-2013 |
20130316419 | METHOD FOR PRODUCING 2,3-BUTANEDIOL BY FERMENTATION - The invention relates to a microorganism strain which has an acetolactate decarboxylase activity 2-12.8 times higher than the non-improved original strain. The invention also relates to a method for producing 2,3-butanediol (2,3-BDL) by fermentation by means of said strain. | 11-28-2013 |
20130330794 | METHOD FOR PRODUCING 2,3-BUTANEDIOL BY FERMENTATION - The invention relates to a production strain for producing 2,3-butanediol. Said production strain has an acetolactate synthase activity 2—68 times higher than the original strain. The invention also relates to a method for producing 2,3-butanediol by fermentation by means of said production strain. | 12-12-2013 |
20140024093 | PROCESS FOR THE PRODUCTION OF DIGESTED BIOMASS USEFUL FOR CHEMICALS AND BIOFUELS - In the pretreatment, the biomass is contacted with a solution containing at least one α-hydroxysulfonic acid thereby at least partially hydrolyzing the biomass to produce a pretreated stream containing a solution that contains at least a portion of hemicelluloses and a residual biomass that contains celluloses and lignin; separating at least a portion of the solution from the residual biomass providing an solution stream and a pretreated biomass stream; then contacting the pretreated biomass stream with a cooking liquor containing at least one alkali selected from the group consisting of sodium hydroxide, sodium carbonate, sodium sulfide, potassium hydroxide, potassium carbonate, ammonium hydroxide, and mixtures thereof and water. A process that allows for higher recovery of carbohydrates and thereby increased yields is provided. Alcohols useful as fuel compositions are also produced from biomass by pretreating the biomass prior to hydrolysis and fermentation. | 01-23-2014 |
20140030780 | METHODS AND ORGANISMS FOR THE GROWTH-COUPLED PRODUCTION OF 1,4-BUTANEDIOL - The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby the one or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO. | 01-30-2014 |
20140030781 | MUTANT MICROORGANISM HAVING IMPROVED 1,4-BDO PRODUCTIVITY AND METHOD OF PREPARING 1,4-BDO USING THE MUTANT MICROORGANISM - A recombinantly modified | 01-30-2014 |
20140113342 | Production of 1,2-Propanediol in Cyanobacteria - Cyanobacterial host cells are modified to produce 1,2-propanediol. | 04-24-2014 |
20140120595 | METHODS, COMPOSITIONS AND SYSTEMS FOR BIOSYNTHETIC BIO-PRODUCTION OF 1,4 BUTANEDIOL - Three biosynthetic pathways are disclosed for microorganism bio-production of 1,4-Butanediol from various carbon sources. Exemplary methods are provided. The recombinant microorganisms comprising any of these 1,4-Butanediol biosynthesis pathways may also comprise genetic modifications directed to improved tolerance for 1,4-Butanediol. | 05-01-2014 |
20140127768 | SOLAR-ASSISTED VOLATILE FERMENTATION PRODUCTS PRODUCTION PROCESSES - The present invention provides a method and apparatus for recapturing heat from a solar-assisted volatile fermentation product production process comprising harvesting a volatile fermentation product from a solar-assisted fermentation product production apparatus and utilizing a heat recovery apparatus for recapturing the heat produced during the solar-assisted fermentation product production process. The volatile fermentation product can be produced in an autotrophic organism or by a fermenting organism fermenting fermentable sugars from one or more sugar crops, starch-containing and lignocellulose-containing materials. | 05-08-2014 |
20140147900 | MICROORGANISMS AND METHODS FOR CONVERSION OF SYNGAS AND OTHER CARBON SOURCES TO USEFUL PRODUCTS - A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. | 05-29-2014 |
20140178954 | EXPRESSION OF XYLOSE ISOMERASE ACTIVITY IN YEAST - Expression of a xylose isomerase in a yeast cell that expresses the chaperonins GroES and GroEL was found to result in enzymatically active xylose isomerase, while there is little to no activity with expression of the bacterial xylose isomerase in a yeast cell lacking GroES and GroEL. A yeast cell expressing xylose isomerase activity, and a complete xylose utilization pathway, provides a yeast cell that can produce a target compound, such as ethanol, butanol, or 1,3-propanediol, using xylose derived from lignocellulosic biomass as a carbon source. | 06-26-2014 |
20140273124 | Method for production of n-propanol and/or ethanol by fermentation of multiple substrates in a symbiotic manner - This invention provides methods and systems for the production of n-propanol and ethanol. Specifically, the methods and systems of the present invention use symbiotic co-cultures for the production of propanol from syngas. | 09-18-2014 |
20140295511 | ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL - A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO. | 10-02-2014 |
20140322777 | PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH - A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO. | 10-30-2014 |
20140322778 | PRODUCTION OF ISOPROPANOL BY IMPROVED RECOMBINANT STRAINS - The expression vector includes: the nucleic acids coding for the polypeptides forming a polypeptide complex having an enzyme activity allowing acetoacetyl-CoA to be converted to acetoacetate; optionally, at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetoacetate to be converted to acetone; and at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetone to be converted to isopropanol; the expression of the nucleic acids being controlled by a single constitutive promoter located upstream of the abovementioned nucleic acids. | 10-30-2014 |
20140322779 | EUKARYOTIC ORGANISMS AND METHODS FOR INCREASING THE AVAILABILITY OF CYTOSOLIC ACETYL-COA, AND FOR THE PRODUCING 1,3-BUTANEDIOL - Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non-naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway, and methods of using such organisms to produce 1,3-BDO. | 10-30-2014 |
20140342418 | XYLITOL PRODUCTION FROM CELLULOSIC BIOMASS - The present disclosure relates to host cells containing a recombinant xylose reductase, a recombinant cellodextrin transporter, a recombinant intracellular β-glucosidase, and lacking xylitol dehydrogenase and xylulokinase, and to methods of using such cells for producing xylitol from cellulosic biomass containing cellodextrin and xylose. | 11-20-2014 |
20140342419 | MICROORGANISM STRAINS FOR THE PRODUCTION OF 2,3-BUTANEDIOL - The present invention is related to a recombinant microorganism engineered for the production of 2,3-butanediol (BDO), wherein said microorganism overexpresses at least one gene encoding a polypeptide involved in the conversion of pyruvate into 2,3-butanediol. The invention is also related to a method of production of 2,3-butanediol comprising the following steps:
| 11-20-2014 |
20150010975 | Extraction Solvents Derived from Oil for Alcohol Removal in Extractive Fermentation - In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol. | 01-08-2015 |
20150024447 | BUTYRALDEHYDE DEHYDROGENASE MUTANT, POLYNUCLEOTIDE ENCODING THE MUTANT, VECTOR AND MICROORGANISM HAVING THE POLYNUCLEOTIDE, AND METHOD OF PRODUCING 1,4-BUTANEDIOL USING THE SAME - A mutant butyraldehyde dehydrogenase (Bld), a polynucleotide having a nucleotide encoding the mutant, a vector including the polynucleotide, a microorganism including a nucleotide encoding the mutant, and a method of producing 1,4-butanediol using the same. | 01-22-2015 |
20150050708 | MICROORGANISMS AND METHODS FOR PRODUCING BUTADIENE AND RELATED COMPOUNDS BY FORMATE ASSIMILATION - Provided herein are non-naturally occurring microbial organisms having a formaldehyde fixation pathway and a formate assimilation pathway, which can further include a methanol metabolic pathway, a methanol oxidation pathway, a hydrogenase and/or a carbon monoxide dehydrogenase. These microbial organisms can further include a butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol. | 02-19-2015 |
20150064758 | MICROORGANISM COMPRISING PYRUVATE DEHYDROGENASE VARIANT AND METHOD OF PRODUCING C4-CHEMICALS USING THE SAME - A recombinant microorganism including pyruvate dehydrogenase having increased activity may increase 1,4-BDO production under anaerobic conditions, as well as a method for preparing same, and method of using same to produce a C4 chemical. | 03-05-2015 |
20150064759 | MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING 2-PROPANOL AND1-PROPANOL AND/OR 1,2-PROPANEDIOL - The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of cytosolic acetyl-CoA to 2-propanol; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of 2-propanol and 1-propanol and/or 1,2-propanediol. | 03-05-2015 |
20150064760 | MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING BUTADIENE AND 1-PROPANOL AND/OR 1,2-PROPANEDIOL - The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of crotonyl alcohol, 5-hydroxy-3-ketovaleryl-CoA, 3-ketopent-4-enoyl-CoA, or 3,5-ketovaleryl-CoA to butadiene; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of butadiene and 1-propanol and/or 1,2-propanediol. | 03-05-2015 |
20150072388 | CONVERSION OF GLYCEROL TO 1,3-PROPANEDIOL UNDER HALOALKALINE CONDITIONS - A method of producing 1,3-propanediol. The method comprises fermenting a haloalkaliphilic species of | 03-12-2015 |
20150079650 | Recombinant microorganisms exhibiting increased flux through a fermentation pathway - The invention provides methods of increasing the production of fermentation products by increasing flux through a fermentation pathway by optimising enzymatic reactions. In particular, the invention relates to identifying enzymes and/or co-factors involved in metabolic bottlenecks in fermentation pathways, and fermenting a CO-comprising substrate with a recombinant carboxydotrophic Clostridia microorganism adapted to exhibit increased activity of the one or more of said enzymes, or increased availability of the one or more of said co-factors, when compared to a parental microorganism. | 03-19-2015 |
20150087037 | FERMENTATION PROCESS - A methods for altering the metabolite profile of a fermentation, by increasing flux through acetolactate. The methods comprises increasing production of one or more products derived from acetolactate. Further provided is a method for increasing the production of 2,3-butandiol by microbial fermentation of gaseous substrates, the method comprising providing a compound which inhibits one or more enzymes which convert acetolactate to branched chain amino acids to the fermentation. The present invention further provides methods for increasing the production of 2,3-butandiol relative to other fermentation products such as ethanol and acetic acid. | 03-26-2015 |
20150087038 | PRODUCTION METHOD FOR 1,4-BUTANEDIOL - An object of the present invention is to provide high-quality 1,4BG capable of working out to a raw material of PBT with good color tone, by efficiently removing and refining impurities mixed when producing a biomass-derived 1,4BG on an industrial scale and the present invention relates to a production method of refined 1,4BG, where a crude 1,4BG-containing solution is obtained from refined raw material 1,4BG obtained by removing bacterial cells, salt contents and water from the fermentation culture medium, through a step of removing high-boiling-point components and/or low-boiling-point components by distillation and/or a step of converting an unsaturated compound to a hydride and the target product is obtained as a side stream in a further distillation step. | 03-26-2015 |
20150093798 | MICROORGANISM CAPABLE OF PRODUCING 1,4-BUTANEDIOL AND METHOD OF PRODUCING 1,4-BUTANEDIOL USING THE SAME - A microorganism capable of producing 1,4-butanediol and a method of producing 1,4-butanediol using the same. | 04-02-2015 |
20150147794 | ETHANE-1,2-DIOL PRODUCING MICROORGANISM AND A METHOD FOR PRODUCING ETHANE-1,2-DIOL FROM D-XYLOSE USING THE SAME - Disclosed herein is a microorganism capable of producing ethane-1,2-diol from D-xylose, and a method for producing ethane-1,2-diol using the same. More specifically, the present invention relates to an engineered | 05-28-2015 |
20150147795 | MICROORGANISM MODIFIED FOR THE PRODUCTION OF 1,3-PROPANEDIOL - The invention relates to a modified microorganism for the production of PDO from a carbon substrate wherein the microorganism includes a three-step metabolic pathway including a first step of conversion of 2,4-dihydroxybutyrate (DHB) to obtain 2-oxo-4-hydroxybutyrate (OHB) by an enzyme having 2,4-DHB dehydrogenase activity, a second step of decarboxylation of the OHB to obtain 3-hydroxypropionaldehyde by an enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, and a third step of reduction of the obtained 3-hydroxypropionaldehyde to obtain PDO with an enzyme having 3-hydroxypropionaldehyde reductase activity and the genes enabling the microorganism for the synthesis of DHB. | 05-28-2015 |
20150291985 | METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE - A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA. | 10-15-2015 |
20150299741 | METHOD FOR CONVERSION OF AN ALKANE OR 1-ALKANOL TO A DIOL - The present invention relates to a method comprising the steps a) providing an alkane or 1-alkanol, b) contacting said alkane or 1-alkanol in an aqueous solution with a cytochrome P450 alkane hydroxylase from the CYP153 family and oxygen for at least 3 hours. | 10-22-2015 |
20150307905 | MANUFACTURING METHOD FOR A BUTANEDIOL, FABRICATION METHOD FOR A MICROBE FOR MANUFACTURING A BUTANEDIOL, AND MICROBE - A method of manufacturing a butanediol through 3-hydroxybutyryl-CoA that uses a microbe and/or a culture thereof and utilizes an enzyme reaction that is caused by an acyl-CoA reductase, wherein the microbe in the manufacturing method for a butanediol is characterized in that an activity of an acyl-CoA hydratase (EC number: 3. 1. 2. −) is deleted or reduced. | 10-29-2015 |
20150337341 | FERMENTATION PROCESS FOR THE PRODUCTION AND CONTROL OF PYRUVATE-DERIVED PRODUCTS - A process for producing and controlling pyruvate derived products during the fermentation of a CO containing substrate by an acetogenic carboxydotrophic microorganism has been developed. The process involves increasing the concentration of at least one nutrient selected from the group consisting of vitamin B1, vitamin B5, vitamin B7 and mixtures thereof above the cellular requirement of the microorganism. When the concentration is increased, the production of 2,3-butanediol (2,3-BDO) increases whereas the production of the other metabolites is virtually unchanged. The effect is reversible so that when the concentration is decreased, the production of 2,3-BDO is also decreased. This allows one to control the ratio of ethanol:2,3-BDO to a desired value which can vary from about 4:1 to about 1:2. | 11-26-2015 |
20150353962 | METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE - A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acyl-CoA reductase, via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA in this order, wherein the reactivity of the acyl-CoA reductase to 4-hydroxybutyryl CoA is greater than or equal to 0.05 times of the reactivity of the acyl-CoA reductase to 3-hydroxybutyryl-CoA. | 12-10-2015 |
20150353963 | PROCESS FOR PREPARING AN ALPHA, OMEGA-ALKANEDIOL - The invention relates to a process for preparing an α,ω-alkanediol comprising the steps of a) reacting an alkanoic acid with an alkanol to give an ester, b) oxidizing at least one terminal carbon atom of the ester by contacting with a whole-cell catalyst, which expresses an alkane hydroxylase, in aqueous solution and in the presence of molecular oxygen, to give an oxidized ester, c) hydrogenating the oxidized ester to form the alkanediol and alkanol, and d) removing the alkanol by distillation, forming a reaction mixture depleted with respect to the alkanol, and recycling the alkanol in step b). | 12-10-2015 |
20150353964 | MUTANTS HAVING CAPABILITY TO PRODUCE 1, 4-BUTANEDIOL AND METHOD FOR PREPARING 1, 4-BUTANEDIOL USING THE SAME - A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process. | 12-10-2015 |
20150368676 | COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS - The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO. | 12-24-2015 |
20150368677 | RECOMBINANT CELL, AND METHOD FOR PRODUCING 1,4-BUTANEDIOL - An object of the present invention is to provide a series of techniques for producing 1,4-butanediol from methanol or the like. Provided is a recombinant cell prepared by introducing a gene encoding at least one enzyme selected from the group consisting of succinate semialdehyde dehydrogenase, succinyl-CoA synthase, CoA-dependent succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, 4-hydroxybutyryl-CoA transferase, 4-hydroxybutyryl-CoA reductase, 4-hydroxybutyraldehyde dehydrogenase, and alcohol dehydrogenase, into a host cell which is a methylotroph, wherein the gene is expressed in the host cell, and the recombinant cell is capable of producing 1,4-butanediol from at least one C1 compound selected from the group consisting of methane, methanol, methylamine, formic acid, formaldehyde, and formamide. | 12-24-2015 |
20150376657 | MANUFACTURING METHOD FOR 1,4-BUTANEDIOL, MICROBE, AND GENE - A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition. | 12-31-2015 |
20160031778 | PROCESS AND SYSTEMS FOR OBTAINING 1,4-BUTANEDIOL FROM FERMENTATION BROTHS - A process of purifying 1,4-butanediol (1,4-BDO) from a fermentation broth including separating solid materials, salts and water, and subjecting the resulting material to a two, three or four column distillation system, that can include a wiped film evaporator to produce a purified 1,4-butanediol product. | 02-04-2016 |
20160040193 | GENETICALLY ENGINEERED BACTERIUM WITH ALTERED CARBON MONOXIDE DEHYDROGENASE (CODH) ACTIVITY - The invention provides genetically engineered microorganisms with altered carbon monoxide dehydrogenase (CODH) activity and methods related thereto. In particular, the invention provides a genetically engineered carboxydotrophic acetogenic bacterium having decreased or eliminated activity of CODH1 and/or CODH2. In certain embodiments, the bacterium may also have increased activity of CODH/ACS. The invention further provides a method for producing a product by culturing the bacterium in the presence of a gaseous substrate comprising one or more of carbon monoxide, carbon dioxide, and hydrogen. | 02-11-2016 |
20160040194 | Process for manufacturing butanediol - A process for the preparation of succinic acid comprising the steps of: | 02-11-2016 |
20160040195 | CONTINUOUS CULTURE FOR 1,3-PROPANEDIOL PRODUCTION USING HIGH GLYCERINE CONCENTRATION - The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a microorganism on a culture medium with high glycerine content. The invention also concerns a new microorganism, or strain of microorganism, adapted for the production of 1,3-propanediol from medium comprising high glycerine content. | 02-11-2016 |
20160053286 | ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL - A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO. | 02-25-2016 |
20160076060 | Microorganisms for Producing 1,3-Butanediol and Methods Related Thereto - Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO. | 03-17-2016 |
20160160223 | RECOMBINANT MICROORGANISMS EXHIBITING INCREASED FLUX THROUGH A FERMENTATION PATHWAY - The invention provides a recombinant, carboxydotrophic | 06-09-2016 |
20190144814 | Genetically Modified Microorganisms | 05-16-2019 |