Entries |
Document | Title | Date |
20080199924 | Production of Degummed Fatty Acid Alkyl Esters - The present invention relates to a method for producing fatty acid alkyl esters, such as fatty acid methyl esters (FAME) and fatty acid ethyl esters with a low level of impurities such as phospholipids. The method of the invention is simplified by combining two process steps into one single process step and is therefore economically cheaper The method includes mixing water, alcohol, triglyceride and/or free fatty acids a lipolytic enzyme and a phospholipase. Subsequently the aqueous phase, which contains glycerine, residual enzyme and most of the hydrolyzed phospholipids, is separated from the non-aqueous phase, whereby the content of phospholipids in the non-aqueous phase is reduced. | 08-21-2008 |
20080199925 | Method For Producing an Aqueous Polymer Dispersion - A process for preparing an aqueous polymer dispersion, in which, in an aqueous medium, in a first reaction stage, a hydroxycarboxylic acid compound is reacted in the presence of an enzyme and of a dispersant, and also, if appropriate, of a low water solubility organic solvent and/or of an ethylenically unsaturated monomer, to give a polyester, and thereafter, in the presence of the polyester, in a second reaction stage, an ethylenically unsaturated monomer is free-radically polymerized. | 08-21-2008 |
20080233620 | Novel Transformant and Process for Producing Polyester Using the Same - The present invention provides a process for producing yeast excellent in cell productivity and gene manipulation of which is easy, being added with nutritional requirement by disrupting only a specific gene, and a transformant thereof. Moreover, the present invention also provides a process for producing a gene expression product, particularly a polyhydroxyalkanoic acid. | 09-25-2008 |
20080248539 | COMPOSITIONS AND METHODS FOR PRODUCING STEREOISOMERICALLY PURE STATINS AND SYNTHETIC INTERMEDIATES THEREFOR - The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith. | 10-09-2008 |
20080280336 | Process for the Preparation of Dihydroxy Esters and Derivatives Thereof - A process is provided for the preparation of a compound of formula (1) | 11-13-2008 |
20080299627 | Method For Production Of High Purity Polyhydroxyalkanonate (PHAs) - The present invention relates to a method of producing polyhydroxyalkanoates (PHAs) using | 12-04-2008 |
20090035831 | Enantioselective immobilized lipase - Immobilization of | 02-05-2009 |
20090047717 | ENZYME-CATALYZED POLYCARBONATE AND POLYCARBONATE ESTER SYNTHESIS - An enzymatic process for preparing aliphatic polycarbonates via terpolymerization or transesterification using a dialkyl carbonate, an aliphatic diester, and an aliphatic diol or triol reactant. A catalyst having an enzyme capable of catalyzing an ester hydrolysis reaction in an aqueous environment is subsequently added to the reaction mixture. Next, polymerization of the reaction proceeds for an allotted time at a temperature ≦100° C. Finally, the copolymer is isolated from an the catalyst via filtration. | 02-19-2009 |
20090075350 | METHODS FOR PRODUCING ALKYL ESTERS - This invention relates to a method for producing an alkyl ester via a transesterification or esterification reaction. The method includes (1) mixing an oil source containing a triglyceride or a carboxylic acid and a first primary alcohol or a first secondary alcohol in a first organic solvent to form a first solution; in which each molecule of the first organic solvent contains 4-8 carbon atoms and a heteroatom; (2) reacting the triglyceride or the carboxylic acid with the first primary alcohol or the first secondary alcohol in the presence of a first lipase to produce a first alkyl ester, in which the first solution does not undergo phase separation throughout the reaction; and (3) separating the first alkyl ester from the first solution. | 03-19-2009 |
20090111153 | METHOD FOR ACYLTRANSFERASE REACTION USING ACYL COENZYME A - The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by a chemical thioester exchange reaction with acylthioester. The present invention, wherein expensive acyl CoA is reproduced nonenzymatically in a reaction system, enables to continuously carry out acyltransferase reaction only by putting a small amount of acyl CoA with a donor and a receptor of an acyl group into a system. Accordingly, the method of the present invention can be applied to an industrial production method of various kinds of compounds including useful biological molecules and synthesis of polymers such as polyester. | 04-30-2009 |
20090123983 | Processes for preparing an intermediate of sitagliptin via enzymatic reduction - The invention provides enzymatic reduction processes for the preparation of 4-(2,4,5-trifluorophenyl)-3-hydroxybutanoate, particularly, (S)-methyl 4-(2,4,5-trifluorophenyl)-3-hydroxybutanoate, a key intermediate in the synthesis of Sitagliptin, and the (S)- and (R)-enantiomers of methyl 4-(2,4,5-trifluorophenyl)-3-hydroxybutanoate in high enantiomeric purity. | 05-14-2009 |
20090130729 | Biocatalytic manufacturing of (meth) acrylic esters - The invention relates to a biocatalytic method or process for the synthesis of esters of acrylic acid and/or methacrylic acid free of positively chargeable/charged groups, comprising reacting one or more alcohols (alcohol starting materials) which are free of positively chargeable or charged groups with (meth)acrylyl-CoA, preferably in the presence of a catalyst (inorganic, organic or organometallic, or preferably a biocatalyst) capable of effecting the transfer of an alcohol radical from an alcohol starting material as defined above or below to (meth)acrylyl CoA under removal of the CoA moiety transferase activity, such as an enzyme of the transferase or hydrolase class of enzymes. The (meth)acrylyl-CoA is preferably formed by reaction of (meth)acrylic acid or its salts with coenzyme A in the presence of an energy providing substance and a biocatalyst e.g. with S-acetyl coenzyme A synthetase activity or by reaction of acrylate or methacrylate produced metabolically in the presence of a biocatalyst or metabolically. | 05-21-2009 |
20090148920 | Integrated glyceride extraction and biodiesel production processes - Biodiesel is used to extract glycerides from biomass derived sources and the extractant containing biodiesel and glycerides is subjected to ester-forming conditions including the presence of lower alkanol to produce biodiesel, a portion of which is used for the extraction of glycerides. | 06-11-2009 |
20090155865 | Esterases and their Use for Processes for Kinetic Resolution of Butinolesters - New enzymes having esterase activity and their use for processes for kinetic resolution of butinolesters. | 06-18-2009 |
20090155866 | METHODS FOR THE SYNTHESIS OF OLEFINS AND DERIVATIVES - The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided. | 06-18-2009 |
20090155867 | Glycolic Acid Production by Fermentation from Renewable Resources - The present invention provides a method for the biological production of glycolic acid from a fermentable carbon source in a microorganism. In one aspect of the present invention, a process for the conversion of glucose to glycolic acid is achieved by the use of a recombinant organism comprising a host | 06-18-2009 |
20090186391 | Method for Production of Erythro-or threo-2-Amino-3-Hydroxypropionic Acid Ester, Novel Carbonyl Reductase, Gene for the Reductase, Vector, Transformant, and Method for Production of Optically Active Alcohol Using Those - The present invention has its object to provide a method of producing an erythro- or threo-2-amino-3-hydroxypropionic acid ester, and so forth. The present invention relates to: a method of asymmetrically reducing an N-2-amino-3-oxopropionic acid ester by allowing cells of a microorganism to act thereon; a polypeptide having an activity of asymmetrically reducing a carbonyl compound to give an optically active alcohol, which is isolated from a microorganism belonging to genus | 07-23-2009 |
20090221044 | Process for the Enantioselective Enzymatic Reduction of Hydroxy Keto Compounds - In a process for the enantioselective enzymatic reduction of a hydroxy ketone of general formula I | 09-03-2009 |
20090233338 | PROCESS FOR RECOVERY OF POLYHYDROXYALKANOATES FROM BIOMASS - Systems and processes for the recovery of polyhydroxyalkanoates (“PHA”) from biomass are disclosed. In certain embodiments, the process includes the steps of extracting at least a portion of PHA from a disrupted quantity of PHA-containing biomass with an acetin solvent to produce a biomass phase (including residual biomass) and a PHA phase including at least a portion of the PHA from the biomass. The biomass phase and the PHA phase are separated, and at least a portion of PHA may be isolated from the PHA phase. Systems and processes including multiple disruption and/or extraction steps also are disclosed. | 09-17-2009 |
20090291480 | Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production - The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof. | 11-26-2009 |
20090298144 | METHODS OF MANUFACTURING DERIVATIVES OF BETA-HYDROXYCARBOXYLIC ACIDS - Preparation of derivatives of β-hydroxycarboxylic acid, including β-hydroxycarboxylic acid esters, α,β-unsaturated carboxylic acid, esters of α,β-unsaturated carboxylic acid, and alkoxy derivatives. | 12-03-2009 |
20090317879 | Use of selection pressures to enable microbial biosynthesis of polyhydroxyalkanoates from anaerobic degradation products - A method for inexpensive and efficient PHA biosynthesis includes operating a sequencing bioreactor in alternating phases of nutrient deprivation and carbon feedstock deprivation to select for robust PHA-producing microbes. Preferably, the bioreactor is operated in a non-sterile manner with mixed cultures of methanotrophs. The method also preferably uses periodic biomass-wasting (PHA harvesting) at the end of the carbon feed phase, gradually lengthening the time period of carbon deprivation phase to create a penalty for rapid PHA degradation and incentive for PHA accumulation. Also, bacterial enrichment cultures may be introduced periodically. The PHA-accumulating bacteria are preferably grown on common anaerobic degradation products, specifically volatile fatty acids, such as acetate and propionate, and methane gas. The PHA has useful applications in bioplastics and other products. | 12-24-2009 |
20100003730 | Enzymatic Synthesis of Acetoacetate Esters and Derivatives - In one embodiment, the present invention includes a method for the synthesis of an ester derivative of acetoacetate. The method includes providing a first ester of acetoacetate and providing an alcohol. The method further includes combining the first ester of acetoacetate and the alcohol in the presence of an enzyme capable of transesterification in a non-aqueous solvent to form the ester derivative of acetoacetate. The method results in the formation of the ester derivative of acetoacetate, which, in one embodiment, is monoacetoacetin. | 01-07-2010 |
20100009419 | MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE - A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms. | 01-14-2010 |
20100021977 | PROCESS FOR PREPARING ALKYL (METH)ACRYLATES USING AN ENZYMATIC CYANOHYDRIN HYDROLYSIS - The present invention relates to a process for preparing alkyl(meth)acrylates, characterized in that the process has a step in which a cyanohydrin is hydrolysed with an enzyme whose residual activity after the conversion of methacrylonitrile in the presence of 20 mM cyanide ions at 20° C. after 30 min is at least 90% of the residual activity of the enzyme which has been used under otherwise identical conditions in the absence of cyanide ions. | 01-28-2010 |
20100035313 | Microorganism capable of producing polyhydroxyalkanoate, polyhydroxyalkanoate synthase, and gene encoding the same - Problem to be Solved: To provide a new microorganism capable of producing a polyhydroxyalkanoate (PHA), a PHA synthase gene, an expression cassette including the gene, a vector including the expression cassette, a transformant transformed by the vector, a polypeptide having PHA synthase activity, a method for producing a PHA synthase and a method for producing a PHA. | 02-11-2010 |
20100055750 | POLYESTER SYNTHESIS - The present disclosure provides for enzymatic polymerization to produce polyester resins suitable for use in manufacturing toners. In embodiments, crystalline copolymers, which are polyesters, may be synthesized from lactones, cyclic anhydrides, cyclic carbonates, and combinations thereof. These crystalline copolymers, in turn, may be utilized in the synthesis of toner particles. | 03-04-2010 |
20100062503 | Lactic acid production from concentrated raw sugar beet juice - The present invention is in the field of the preparation of lactic acid by means of fermentation on industrial scale wherein a concentrated raw beet juice having a Brix of at least 60 is used as fermentation substrate. After dilution to the desired initial sugar concentration and addition of nutrients, the juice is fermented to lactic acid and/or lactate by means of a lactic acid-producing microorganism. Said concentrated raw beet juice is prepared by: washing and cutting sugar beet and extracting the cossettes in water, removing the beet pulp from the resulting raw beet juice, and heat treating the raw beet juice at a temperature between 50 and 90 degrees Celsius, and concentrating the raw beet juice to at least 60 Brix. | 03-11-2010 |
20100068773 | MICROBIOLOGICAL PRODUCTION OF 3-HYDROXYISOBUTYRIC ACID - The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or poly-hydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide. | 03-18-2010 |
20100081179 | INCREASED HETEROLOGOUS Fe-S ENZYME ACTIVITY IN YEAST - Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol. | 04-01-2010 |
20100099155 | RECOVERY OF VOLATILE PRODUCTS FROM FERMENTATION BROTH - Provided are apparatuses and processes for the removal and purification of fermentation prepared one or more volatile organic compounds. The apparatuses comprise a fermentor unit, a vacuum side stripper unit ( | 04-22-2010 |
20100105114 | Methods and Compositions for Regulating Sporulation - Methods and compositions are provided for producing fuel utilizing various strains of | 04-29-2010 |
20100105115 | ALCOHOL PRODUCTION PROCESS - The invention relates to biological processes for producing one or more desired products, including alcohols such as ethanol and butanol. The processes comprise carrying out first and second fermentations of substrates in first and second bioreactors, wherein each fermentation produces one or more desired products and/or one or more by-products that can be utilised in the other fermentation. A product and/or by-product of the first fermentation is introduced to the second bioreactor during the fermentation, and a product and/or by-product of the second fermentation is introduced to the first bioreactor during the fermentation. | 04-29-2010 |
20100124773 | METHODS AND PROCESSES FOR PRODUCING ESTERS - Methods are provided for producing esters. The methods comprise converting a fermentable carbon source to organic acids by fermentation with organic acid producing microorganisms, followed by catalytic esterification. The methods comprise integrated fermentation, extraction, and esterification reactions wherein the organic acids produced during fermentation are extracted into an extraction solvent and then directly reacted with an alcohol in the presence of a catalyst to form organic esters. Methods of producing esters are also provided wherein the organic acids produced during fermentation and extracted into the extraction solvent are stripped from the extraction solvent prior to being reacted with an alcohol in the presence of a catalyst to form organic esters. | 05-20-2010 |
20100136637 | RECOMBINANT MICROORGANISM HAVING A PRODUCING ABILITY OF POLYLACTATE OR ITS COPOLYMERS AND METHOD FOR PREPARING POLYACTATE OR ITS COPOLYMERS USING THE SAME - Provided are a recombinant microorganism capable of producing polylactate (PLA) or hydroxyalkanoate-lactate copolymers and a method of preparing PLA or hydroxyalkanoate-lactate copolymers using the same. The recombinant microorganism has both a gene encoding a propionyl-CoA transferase from | 06-03-2010 |
20100136638 | PRODUCTION OF 3-HYDROXYPROPIONIC ACID USING BETA-ALANINE/PYRUVATE AMINOTRANSFERASE - Methods of using beta-alanine/pyruvate aminotransferase to produce 3-hydroxypropionic acid and derivatives thereof, from beta-alanine, are disclosed. Cells and recombinant nucleic acids that can be used to practice the methods are also disclosed. | 06-03-2010 |
20100143987 | PROCESS FOR PREPARING GLYCEROL ESTERS - There is provided a process for the preparation of a compound of the formula, wherein one of R | 06-10-2010 |
20100151541 | NOVEL POLYPEPTIDE HAVING ESTERASE ACTIVITY AND RECOMBINANT ESTERASE AND USE THEREOF - Polypeptide and recombinant protein having esterase activity which exhibit the amino acid sequence SEQ. ID. No. 1 and the use thereof. | 06-17-2010 |
20100151542 | Cleaning Enzymes and Fragrance Production - The present invention provides compositions comprising an acyltransferase and an alcohol substrate for the acyl-transferase. In some particularly preferred embodiments, the composition finds use in production of a fragrant ester. In some other embodiments, the composition finds use in laundry detergents to clean stains that contain at least one triglyceride. In some further embodiments, the compositions are used to produce compounds with cleaning properties (e.g., a surfactant ester). | 06-17-2010 |
20100173370 | Fuel Production - An apparatus that includes a first reactor and a return mechanism. The first reactor has an inlet to receive a mixture comprising a first reactant, a second reactant, a reaction product, and an inert solvent that dissolves at least a portion of the first and second reactants, an enzyme to facilitate a reaction between the first and second reactants to generate more reaction product, and an outlet to output the reaction product, including the reaction product received at the inlet and the reaction product generated from the reaction between the first and second reactants. The return mechanism sends at least a portion of the reaction product from the outlet back to the inlet. | 07-08-2010 |
20100178678 | Method for producing cyclopent-4-ene-1,3-diol or cyclopent-4-ene-1,3-diol derivatives - The invention relates to a method for producing cis-cyclopent-4-ene-1,3-diol and cis-cyclopent-4-ene-1,3-dialkanoates by selective cis-1,2-reduction of 4-hydroxycyclopent-2-enone by means of a hydroboron, in the presence of substoichiometric quantities of a trivalent rare earth metal compound, to form cyclopent-4-ene-1,3-diol which can then be optionally reacted with an acylation agent to form cis-cyclopent-4-ene-1,3-dialkanoates, in order to simplify the reprocessing and without intermediate isolation. | 07-15-2010 |
20100190221 | METHOD FOR PRODUCING POLYHYDROXYALKANOIC ACID - Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production. | 07-29-2010 |
20100196972 | PRODUCING ENERGY FROM BIOLOGICAL MATERIAL - Described are methods and systems for producing synthesis gas. In some embodiments, the method includes microbiologically converting biological material to form methane and CO2; and reacting methane and CO2 formed in (a) with water and metal to form synthesis gas. Optionally, the method also includes cutting vegetation; fermenting the vegetation to form biogas comprising methane and CO2; and reacting the biogas with water and metal to form synthesis gas and metal oxide. In some embodiments of the invention, reacted metal is regenerated from metal oxide produced in the reaction. In some embodiments the regeneration comprises reacting the oxide in a bath of boiling zinc. | 08-05-2010 |
20100196973 | METHODS FOR THE SYNTHESIS OF FATTY DIACIDS BY THE METATHESIS OF UNSATURATED DIACIDS OBTAINED BY FERMENTATION OF NATURAL FATTY ACIDS - The invention relates to a method for the synthesis of diacids of general formula ROOC—(CH | 08-05-2010 |
20100196974 | Chemically Inducible Expression of Biosynthetic Pathways - Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent. | 08-05-2010 |
20100203602 | Materials and Methods for Efficient Lactic Acid Production - The present invention provides derivatives of | 08-12-2010 |
20100209983 | PREPARING METHOD FOR (S)-3HYDROXYBUTYRIC ACID AND (S)-3 HYDROXYBUTYRATE ESTER USING RECOMBINANT MICROORGANISM - A method of synthesizing optically-active (S)-3-hydroxybutyric acid and (S)-3-hydroxybutyrate ester using a mutated microorganism is provided. More particularly, a mutated microorganism for preparing (S)-3-hydroxybutyric acid transformed with a gene encoding b ketothiolase, a gene encoding (S)-3-hydroxybutyryl CoA dehydrogenase and a gene encoding acyl CoA hydrolase; a method of preparing (S)-3-hydroxybutyric acid using the mutated microorganism; a mutated microorganism for preparing (S)-3-hydroxybutyrate ester transformed with a gene encoding b ketothiolase, a gene encoding (S)-3-hydroxybutyryl CoA dehydrogenase, a gene encoding acyl CoA hydrolase and a gene encoding lipase; and a method of preparing (S)-3-hydroxybutyrate ester using the mutated microorganism are provided. | 08-19-2010 |
20100209984 | PROCESS FOR THE PREPARATION OF DIHYDROXY ESTERS AND DERIVATIVES THEREOF - A process is provided for the preparation of a compound of formula (1) | 08-19-2010 |
20100233768 | METHOD FOR ISOLATING POLYHYROXYALKANOATES - The invention relates to a method for isolating polyhydroxyalkanoates from production cells which comprises | 09-16-2010 |
20100240107 | PROCESS FOR PRODUCING OPTICALLY ACTIVE 2-ALKYL-1,1,3-TRIALKOXYCARBONYLPROPANE - A process for producing an optically active 2-alkyl-1,1,3-trialkoxycarbonylpropane (2), comprising a step of asymmetric hydrolysis of 2-alkyl-1,1,3-trialokoxycarbonylpropane (1) by using an enzyme capable of selectively hydrolyzing an ester moiety of either one enantiomer of 2-alkyl-1,1,3-trialkoxycarbonylpropane (1), or by using a culture of a microorganism capable of producing the enzyme or a treated object thereof. | 09-23-2010 |
20100248318 | HYDROGEN PROCESSING AND IMPURITY REMOVAL AND CLEANING METHODS IN A BIOMASS CONVERSION PROCESS - In one embodiment, the disclosure includes a method of biomass conversion including fermenting biomass to produce a carboxylic acid or carboxylate salt and hydrogen gas, recovering the hydrogen gas, and converting the carboxylic acid or carboxylate salt to an alcohol using the hydrogen gas. In one embodiment, the hydrogen produced by biomass conversion may be converted to an acetate. Another embodiment relates to a biomass conversion system. The system may include: a fermentation unit for fermentation of biomass to a carboxylic acid or carboxylate salt in a fermentation broth and for production of a carbon dioxide and hydrogen gas stream, an extraction unit for extracting the carboxylic acid or carboxylate salt from the fermentation broth, a gas extraction unit for separation of the hydrogen gas and the carbon dioxide, and a production unit for production of an alcohol from the carboxylic acid or carboxylate salt using the hydrogen gas. | 09-30-2010 |
20100261236 | Genetically Stable Plasmid Expressing PDH and FDH Enzymes - Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided. | 10-14-2010 |
20100273223 | New Calb Muteins and Their Use - A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A. | 10-28-2010 |
20100291644 | PROCESS FOR PREPARING METHACRYLIC ACID OR METHACRYLIC ESTERS - The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of
| 11-18-2010 |
20100311131 | TRANSGENIC SYSTEMS FOR THE MANUFACTURE OF POLY(2-HYDROXY-BUTYRATE-CO-3-HYDROXYHEXANOATE) - Methods for engineering transgenic organisms that synthesize polyhydroxyalkanoates (PHAs) containing 3-hydroxyhexanoate as comonomer have been developed. These processes are based on genetically engineered bacteria such as | 12-09-2010 |
20110008855 | Recombinant Microorganism Capable Of Producing Polylactate Or Polylactate Copolymer From Sucrose And Method For Producing Polylactate Or Polylactate Copolymer From Sucrose Using The Same - Provided are a microorganism capable of producing a polylactate (PLA) or PLA copolymer from sucrose and a method of producing the PLA or PLA copolymer from sucrose using the same. A gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate are introduced into a microorganism capable of using sucrose as a substrate, and the microorganism is cultured using sucrose as the substrate, thereby allowing efficient production of a PLA or PLA copolymer. | 01-13-2011 |
20110027843 | METHOD FOR PRODUCING POLYHYDROXYALKANOATE - A method for producing polyhydroxyalkanoate (PHA), comprising (i) culturing in a culture medium comprising terephthalic acid and/or a salt thereof and/or an ester thereof one or more bacterial strains which are capable of accumulating PHA from terephthalic acid or a salt or ester thereof and which are selected from | 02-03-2011 |
20110039315 | Methods and Systems for Biomass Conversion to Carboxylic Acids and Alcohols - The disclosure includes a method, process and apparatus for the conversion of biomass to carboxylic acids and/or primary alcohols. The system may include a pretreatment/fermentation subsystem operable to produce a fermentation broth containing carboxylic acid salts from biomass, such as lignocellulosic biomass. The system may also include a dewatering subsystem operable to remove excess water from the fermentation broth to produce a concentrated product. The system may also includes an acid springing subsystem operable to produce a mixed carboxylic acid product. The system may also include a hydrogenation subsystem operable to produce an alcohol mixture, such as a mixture containing primary alcohols. Methods of operating this system or other systems to obtain a carboxylic acid or alcohol mixture are also provided. | 02-17-2011 |
20110045557 | Novel Fusion Carbonic Anhydrase/Cellulose Binding Polypeptide Encoded by a Novel Hybrid Gene, and Method of Creating and Using the Same - The invention relates a novel hybrid carbonic anhydrase catalyst with the potential to contribute significantly to meeting targeted reductions in greenhouse gas emissions. In a preferred embodiment of the present invention at least a portion of a cellulose binding domain (CBD) of a protein is fused to another protein, carbonic anhydrase, (CA) to create a new multi-functional protein which can bind tightly to cellulose while maintaining its native catalytic ability to process CO | 02-24-2011 |
20110081692 | POLYHYDROXYALKANOIC ACID COPOLYMER AND PROCESS FOR PREPARING SAME - A PHA copolymer comprising (R)-3-hydroxy-4-methyl valeric acid units and a production method thereof are provided. | 04-07-2011 |
20110104767 | METHOD FOR PRODUCING POLYHYDROXYALKANOATE (PHAs) USING HALOBACTERIUM AND HALOBACTERIUM - Disclosed are a method for producing polyhydroxyalkanoates (PHAs) using a halobacterium belonging to the genus | 05-05-2011 |
20110104768 | METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM - The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material. | 05-05-2011 |
20110124064 | ESTERIFICATION PROCESS OF PROSTAGLANDINS AND ANALOGUES THEREOF - The invention relates to a process for enzymatically catalyzed esterification of prostaglandins or analogues thereof. | 05-26-2011 |
20110159555 | Methods, Chimeric Polypeptides, Polynucleotides and Cells for Producing Bioplastics - Methods of producing a bioplastic are disclosed. Furthermore, chimeric polypeptides useful to transport polyhydroxyalkanoates (PHAs) are disclosed, as are polynucleotides that code for the chimeric polypeptides, cells harboring the chimeric polypeptides and methods of transporting PHAs. | 06-30-2011 |
20110159556 | Use of hydroxyalkanoic acids as substrates for production of poly-hydroxyalkanoates by methane-oxidizing bacteria - A method of biosynthesis of polyhydroxyalkanoates (PHA) is provided that includes providing a type II methanotrophic bacteria, and disposing the type II methanotrophic bacteria in an unbalanced growth condition, where the unbalanced growth condition includes a nutrient-deficient media and a hydroxyalkanoic acid, and where the nutrient-deficient media has an absence of an essential nutrient required for cell replication of the type II methanotrophic bacteria. | 06-30-2011 |
20110165640 | METHOD FOR THE ENZYMATIC PRODUCTION OF 2-HYDROXY-2-METHYL CARBOXYLIC ACIDS - Provided is a composition containing: an isolated and purified polypeptide containing the amino acid sequence of SEQ ID NO: 4; and/or a heterodimeric enzyme containing: the isolated and purified polypeptide containing the amino acid sequence of SEQ ID NO: 4; and an isolated and purified polypeptide containing the amino acid sequence of SEQ ID NO: 2. Also provided is a process for producing a 2-hydroxy-2-methyl carboxylic acid or a salt or ester thereof, wherein the process involves: contacting a 3-hydroxy carboxylic acid or a salt or ester thereof with the above-mentioned composition to produce the 2-hydroxy-2-methyl carboxylic acid or the salt or ester thereof. | 07-07-2011 |
20110171702 | RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID - The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor. | 07-14-2011 |
20110177569 | MUTANT OF PROPIONYL-COA TRANSFERASE FROM CLOSTRIDIUM PROPIONICUM AND PREPARING METHOD FOR PLA OR PLA COPOLYMER USING THE SAME - Provided is a mutant of propionyl-CoA transferase from | 07-21-2011 |
20110183388 | Extracellular Polyhydroxyalkanoates Produced By Genetically Engineered Microorganisms - The present invention is in the field of biosynthesis of polyhydroxyalkanoates (PHA). The invention relates to a genetically engineered microorganism having at least one gene involved in the metabolism, preferably in the production, of polyhydroxyalkanoates (PHA). This microorganism is useful in commercial production of polyhydroxyalkanoates. The present invention further relates to a method for the production of polyhydroxyalkanoates (PHA). | 07-28-2011 |
20110201067 | METHOD FOR PREPARING POLYLACTATE AND COPOLYMER THEREOF USING A MUTANT MICROORGANISM WITH ENHANCED POLYLACTATE, AND THE COPOLYMER PRODUCING CAPABILITY THEREOF - Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful. | 08-18-2011 |
20110207186 | Isoforms of Pig Liver Esterase - The invention relates to novel mutants of γPLE, to vehicles containing the same and to their use in the production of enantiomer-enriched alcohols, carboxylic acids and esters. | 08-25-2011 |
20110212497 | METHOD FOR PRODUCTION OF POLYLACTATE USING RECOMBINANT MICROORGANISM - It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material. | 09-01-2011 |
20110236936 | METHOD FOR ENHANCING PRODUCTION POLYHYDROXYALKANOIC ACID FROM MICROORGANISM STRAINS - Provided is a method for enhancing the production of polyhydroxyalkanoic acid (PHA) from microorganism strains by disrupting a gene associated with the production of an exobiopolymer (EBP) in the | 09-29-2011 |
20110275127 | Process for Producing Ethanol - A process for producing ethanol including a combination of biochemical and synthetic conversions results in high yield ethanol production with concurrent production of high value coproducts. An acetic acid intermediate is produced from carbohydrates, such as corn, using enzymatic milling and fermentation steps, followed by conversion of the acetic acid into ethanol using esterification and hydrogenation reactions. Coproducts can include corn oil, and high protein animal feed containing the biomass produced in the fermentation. | 11-10-2011 |
20110287498 | PROCESSING BIOMASS - Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels, carboxylic acids and equivalents thereof (e.g., esters and salts). For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol, butanol or organic acids (e.g., acetic or lactic acid), salts of organic acids or mixtures thereof. If desired, organic acids can be converted into alcohols, such as by first converting the acid, salt or mixtures of the acid and its salt to an ester, and then hydrogenating the formed ester. Acetogens or homoacetogens which are capable of utilizing a syngas from a thermochemical conversion of coal or biomass can be utilized to produce the desired product. | 11-24-2011 |
20110294176 | METHOD OF EXTRACTING BUTYRIC ACID FROM A FERMENTED LIQUID AND CHEMICALLY CONVERTING BUTYRIC ACID INTO BIOFUEL - Disclosed is a method of converting butyric acid contained in a fermentation broth into biofuel. This chemical conversion method includes separating biohydrogen from gases generated in the course of production of butyric acid through fermentation of carbohydrate, extracting butyric acid from the broth using an insoluble solvent, esterifying butyric acid thus producing butylbutyrate, and hydrogenolyzing all or part of butylbutyrate, thus obtaining butanol. Thereby, biobutanol can be efficiently and economically produced, and butylbutyrate, which has oxidation stability superior to that of conventional biodiesel (fatty acid methyl ester) and is thus regarded as novel biofuel, can be produced together. | 12-01-2011 |
20110300592 | METHOD FOR PRODUCING POLY-3-HYDROXYALKANOIC ACID - When industrially separating and purifying poly-3-hydroxyalkanoic acid produced by a microorganism, to obtain poly-3-hydroxyalkanoic acid particles having an arbitrary volume mean particle diameter with favorable productivity and with decreased amount of an organic solvent used is enabled while decreasing contaminants derived from constitutive components of cellular bodies. According to the present invention, aggregated matter of poly-3-hydroxyalkanoic acid is obtained by adjusting the amount of organic nitrogen in an aqueous suspension containing poly-3-hydroxyalkanoic acid to not greater than 1,500 ppm per weight of poly-3-hydroxyalkanoic acid; and thereafter allowing poly-3-hydroxyalkanoic acid to be aggregated in the aqueous suspension. | 12-08-2011 |
20120009636 | Methods and Compositions for the Recombinant Biosynthesis of Fatty Acids and Esters - The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms. | 01-12-2012 |
20120021471 | BIOABSORBABLE POLYMER CONTAINING 2-HYDROXYACID MONOMERS - Genetically engineered organisms for production of PHA copolymers containing 2-hydroxyacid monomers and the methods of making and using thereof have been developed. The copolymers containing 2-hydroxyacid monomers can be synthesized via biosynthesis by the action of a PHA polymerase in a living cell. By changing the genetic background of the cells, one can control specific metabolic pathways allowing control of the level of glycolic acid co-monomer in the PHA polymer. | 01-26-2012 |
20120028320 | PREPARATION OF ADIPIC ACID - The invention relates to a method for preparing adipic acid, comprising converting alpha-ketoglutaric acid (AKG) into alpha-ketoadipic acid (AKA), converting alpha-ketoadipic acid into alpha-ketopimelic acid (AKP), converting alpha-ketopimelic acid into 5-formylpentanoic acid (5-FVA), and converting 5-formylpentanoic acid into adipic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst.The invention further relates to a heterologous cell, comprising one or more heterologous nucleic acid sequences encoding one or more heterologous enzymes capable of catalysing at least one reaction step in said method. | 02-02-2012 |
20120045801 | RECOMBINANT RALSTONIA EUTROPHA CAPABLE OF PRODUCING POLYLACTIC ACID OR POLYLATIC ACID COMPOLYMER, AND METHOD FOR PRODUCING POLYLACTIC ACID OR POLYLATIC ACID COPOLYMER USING SAME - Provided are a recombinant | 02-23-2012 |
20120045802 | Liquid fractionation method for producing biofuels - A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, sequentially adding solvent sets to the algal biomass, and sequentially separating solid biomass fractions from liquid fractions to arrive at a liquid fraction comprising neutral lipids. The method also includes esterifying the neutral lipids, separating a water miscible fraction comprising glycerin from a water immiscible fraction comprising fuel esters, carotenoids, and omega-3 fatty acids. The method also includes obtaining a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock. | 02-23-2012 |
20120077236 | 3-HYDROXYPROPIONIC ACID AND OTHER ORGANIC COMPOUNDS - Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed. | 03-29-2012 |
20120083021 | Low odor coating compositions and paints - This invention provides low odor stable coating compositions, and methods of their production. The stable aqueous coating compositions and paints comprise one or more emulsion-polymerized addition polymer and one or more carboxylesterase enzyme having an ester hydrolysis activity in the composition of less than 0.03 micromole/minute, wherein the aqueous coating composition has a headspace volatile organic compound (VOC) content, as measured by headspace gas chromatography-mass spectrometry (GC-MS) at 33° C., of less than 10 ppm of the one or more organic carboxylester with a normal boiling point of less than 150° C. and more than 50 ppm of the one or more mono-alcohol with a formula molecular weight of less than 76. | 04-05-2012 |
20120088280 | GENE ENCODING POLYMER SYNTHASE AND A PROCESS FOR PRODUCING POLYMER - An isolated polynucleotide encoding for a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 1 with polymer synthase activity. | 04-12-2012 |
20120100582 | PIG LIVER ESTERASES - The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in | 04-26-2012 |
20120122165 | RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER WITH THE USE OF THE SAME - This invention provides a recombinant microorganism exhibiting excellent aliphatic polyester productivity and a method for producing an aliphatic polyester with the use of the recombinant microorganism. In this invention, the | 05-17-2012 |
20120135480 | METHODS FOR PRODUCING POLYHYDROXYALKANOATES FROM BIODIESEL-GLYCEROL - Methods are provided for producing biodegradable polyhydroxyalkanoates (PHAs) with desired geometry, molecular mass, mechanical and/or physical-chemical properties from glycerol, an inexpensive carbon source and byproduct of the biodiesel industry. Microorganisms capable of converting carbon to PHA can be used to convert biodiesel-glycerol to poly-3-hydroxybutyrate (PHB) or other monomer or copolymer PHAs via fermentation. The microorganisms are cultured in a medium comprising glycerol as a primary carbon source and one or more low molecular mass organic acids as a secondary carbon source. Biomass can be harvested from the culture medium and crude PHA extracted and purified, thereby recovering purified PHA with the desired property. After PHA isolation, a nucleating agent can be added to improve certain physical-chemical properties of the PHA; e.g., crystallization temperature, to enhance performance of the PHA during injection molding. | 05-31-2012 |
20120149076 | INTEGRATION OF FERMENTAITON WITH MEMBRANE - Herein disclosed is a method comprising a) fermenting biomass to produce a fermentation broth; b) separating the fermentation broth into a liquid stream and a solid or slurry stream; c) passing the liquid stream through a reverse osmosis membrane to obtain a permeate and a retentate; and d) concentrating the retentate. Herein disclosed is a method comprising a) fermenting biomass in a first fermentor to produce a first fermentation broth; b) separating the first fermentation broth into a first liquid stream and a first solid or slurry stream; c) introducing the first solid or slurry stream into a second fermentor to produce a second fermentation broth, wherein the second fermentor comprises a lower fermentation products concentration than the first fermentor; d) separating the second fermentation broth into a second liquid stream and a second solid or slurry stream; and e) passing the second liquid stream through a reverse osmosis membrane. | 06-14-2012 |
20120156738 | PRODUCTION OF ALCOHOL ESTERS AND IN SITU PRODUCT REMOVAL DURING ALCOHOL FERMENTATION - An alcohol fermentation process and composition that includes production of alcohol esters by esterification of product alcohol in a fermentation medium with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the product alcohol recovered from the alcohol esters. The carboxylic acid can also serve as an extractant for removal of the alcohol esters from the fermentation medium. | 06-21-2012 |
20120171737 | PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS - Mutants of various polyhydroxyalkanoate (PHA) synthases capable of synthesizing a lactate polymer (PLA) and a lactate copolymer (PLA copolymer), and a method of preparing a lactate polymer and a lactate copolymer using the same are provided. More specifically, a mutant of polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8, and a method of preparing lactate polymer and lactate copolymer using the mutant of synthase are provided. The polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8 can have an activity of synthesizing a lactate polymer and a lactate copolymer by an amino acid sequence mutation affecting an activity of synthesizing a lactate polymer, and can produce a lactate polymer and a copolymer that have different features, respectively, by using the mutants of the synthase. | 07-05-2012 |
20120171738 | ENZYMATICALLY CATALYZED METHOD OF PREPARING MONO-ACYLATED POLYOLS - The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacyl-glycerol lipase mutants, as for example derived from | 07-05-2012 |
20120214213 | RECOMBINANT HOSTS AND METHODS FOR MANUFACTURING POLYHYDROXYALKANOATE - Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (P | 08-23-2012 |
20120237988 | BUTANOL STRAIN IMPROVEMENT WITH INTEGRATION OF A POLYNUCLEOTIDE ENCODING A POLYPEPTIDE THAT CATALYZES PYRUVATE TO ACETOLACTATE CONVERSION - The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells. | 09-20-2012 |
20120244586 | 3-HYDROXYPROPIONIC ACID AND OTHER ORGANIC COMPOUNDS - Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed. | 09-27-2012 |
20120252081 | USING CELL DEBRIS GENERATED FROM PHA RECOVERY FOR ENHANCED CELL GROWTH AND BIOPOLYESTER FORMATION - The present invention relates to a process for producing biodegradable polymeric materials including polyhydroxyalkanoates (PHAs) by using the cell debris left from PHA recovery and purification. The process comprises: (a) cultivating PHA-producing microbial cells in a medium solution containing an organic carbon source to form PHAs that are accumulated in the cells as inclusion bodies; (b) harvesting the cells from the spent medium and solubilizing the non-PHA cell mass to obtain a PHA solid and a cell debris solution; (c) separating the PHA solid from the cell debris solution; (d) feeding the cell debris solution to the cultivation step (a). By reusing the cell debris generated from PHA recovery, the invention avoids disposal of a large amount of aqueous waste. In addition, a remarkable increase of cell growth and PHA synthesis is achieved, because the cell debris can be readily assimilated by the microbial cells as the nutrients. | 10-04-2012 |
20120258506 | CELL-BASED SYSTEMS FOR PRODUCTION OF METHYL FORMATE - Disclosed is a process in which a recombinant organism, such as a yeast, expressing a heterologous S-adenosylmethionme (SAM)-dependent methyl halide transferase (MHT) protein is combined with a halide and a carbon source in a cultivation medium under conditions in which methyl formate is produced. The cell may genetically modified to express methyl formate synthase, methanol dehydrogenase and/or hydrolytic dehalogenase at levels higher than a cell of the same species that is not genetically modified. The methyl formate may be collected and used in a variety of applications. The halide may be chlorine, bromine or iodine. | 10-11-2012 |
20120258507 | DETERGENT COMPOSITIONS CONTAINING THERMOBIFIDA FUSCA LIPASE AND METHODS OF USE THEREOF - The present compositions and methods relate to a lipase cloned from | 10-11-2012 |
20120264181 | METHOD FOR PRODUCING BUTYRIC ACID, BUTANOL AND BUTYRATE ESTER - The present disclosure is directed to methods for producing butyric acid comprising fermenting a feedstock using a bacterium. The feedstock comprises lactic acid, or the feedstock comprises lactic acid and at least one carbohydrate. | 10-18-2012 |
20120264182 | USE OF A PROTEIN HOMOLOGOUS TO A MEAB PROTEIN FOR INCREASING THE ENZYMATIC ACTIVITY OF A 3-HYDROXYCARBOXYLIC ACID-COA MUTASE - The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid. | 10-18-2012 |
20120270285 | STEREOSPECIFIC CARBONYL REDUCTASES - Stereospecific carbonyl reductases SCR1, SCR2, and SCR3 are described herein as are nucleotide sequences that encode these reductases. These stereospecific carbonyl reductases have anti-Prelog selectivity and have specificities that are useful for fine biochemical synthesis. | 10-25-2012 |
20120288907 | Glucose Transport Mutants for Production of Biomaterial - A method is disclosed for restoring a Glu | 11-15-2012 |
20120301933 | PHA-PRODUCING BACTERIA - The present disclosure relates to isolated bacterial strains producing polyhydroxyalkanoate (PHA), microbial consortia including such strains, and cultures of such strains and microbial consortia. In particular, the present disclosure relates to compositions including such strains, microbial consortia, and cultures and methods of use thereof. | 11-29-2012 |
20120301934 | Process for the Production of Esters - Methods for producing an ester from a gas comprising carbon monoxide. More specifically the invention provides for methods for producing one or more products by the anaerobic fermentation of a gaseous substrate comprising CO and the subsequent conversion of the one or more products to an ester in a down-stream reaction. The invention further provides methods for improving the efficiency of the production of esters from gaseous substrates. | 11-29-2012 |
20130005006 | METHOD FOR PRODUCING POLYHYDROXYALKANOIC ACID - Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production. | 01-03-2013 |
20130017583 | METHODS FOR PRODUCING POLYHYDROXYALKANOATE COPOLYMER WITH HIGH MEDIUM CHAIN LENGTH MONOMER CONTENT - The invention relates to the production of polyhydroxyalkanoate copolymer with high 3-hydroxyhexanoate monomer content through recombinant gene expression. | 01-17-2013 |
20130029388 | Method for Accumulation of Polyhydroxyalkanoates in Biomass with On-Line Monitoring for Feed Rate Control and Process Termination - A method or process for producing polyhydroxyalkanoates (PHAs) in biomass. The process entails feeding an organic carbon containing substrate to biomass enriched in PHA accumulating bacteria. Particularly the process entails intermittently supplying the substrate to the biomass at least three separate times over a selected period. The object of the process is to produce PHA having a relatively high molecular weight, at least 400,000 g/mole. By controlling the frequency at which the substrate is supplied to the biomass and by feeding a sufficient amount of the substrate to the biomass, the method or process produces the PHA having the relatively high molecular weight. | 01-31-2013 |
20130040350 | Process for the Direct Production of Fermentation Products from Biomasses in a Biofilm Reactor - A dense but oxygen permeable membrane separates the oxygen supply compartment from the fermentation compartment, which contains all microorganisms, a nutrient medium and the pretreated lignocellulose. The oxygen, necessary for the growth and the activity of the aerobic cellulolytic enzymes producing microorganisms is solely transported from the oxygen supply compartment through the membrane, which leads to an oxygen gradient within the biofilm growing on the membrane. The oxygen rich zone of the biofilm lies on the membrane whereas the biofilm further away from the membrane as well as the surrounding nutrient medium are oxygen depleted. In the aerobic biofilm the extra-cellular enzymes are produced in situ and are released into the nutrient medium where they hydrolyse the cellulose and hemicellulose into soluble monosugars, which are then converted to the desired fermentation product by suitable microorganisms in the anaerobic zones of the reactor. | 02-14-2013 |
20130045516 | RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER USING THE SAME - Aliphatic polyester productivity is improved for production of aliphatic polyester using a recombinant microorganism. A recombinant microorganism prepared by introducing a gene encoding a protein having activity of converting lactic acid to lactic-acid CoA and a gene encoding a protein having activity of synthesizing polyhydroxyalkanoate using hydroxyacyl CoA as a substrate into a host microorganism is cultured and then aliphatic polyester is recovered from the medium. | 02-21-2013 |
20130065284 | MODIFIED MICROORGANISM FOR HIGHLY EFFICIENT PRODUCTION OF LACTIC ACID - A modified microorganism for high efficient production of lactic acid, an expression vector for constructing the modified microorganism, and a method of producing a lactic acid using the same are disclosed. The modified microorganism may produce lactic acid at a high level under acid conditions. | 03-14-2013 |
20130065285 | Chemoautotrophic Conversion of Carbon Oxides in Industrial Waste to Biomass and Chemical Products - Systems and methods for employing chemoautotrophic micro-organisms to capture carbon from industrial waste are provided. An exemplary system comprises an industrial source, such as a cement plant, and a bioreactor including the micro-organisms. The bioreactor is fed the waste stream from the source, which provides carbon to the micro-organisms, and is also fed hydrogen, from which the micro-organisms derive their energy. Additional or alternative carbon can be provided from a gasifier fed an organic feedstock. The carbon provided to the micro-organisms is converted into chemical products which can be recovered from the bioreactor. Hydrogen can be produced by electrolysis using electricity generated by a renewable energy source. | 03-14-2013 |
20130065286 | PROCESSING BIOMASS - Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels, carboxylic acids and equivalents thereof (e.g., esters and salts). For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol, butanol or organic acids (e.g., acetic or lactic acid), salts of organic acids or mixtures thereof. If desired, organic acids can be converted into alcohols, such as by first converting the acid, salt or mixtures of the acid and its salt to an ester, and then hydrogenating the formed ester. Acetogens or homoacetogens which are capable of utilizing a syngas from a thermochemical conversion of coal or biomass can be utilized to produce the desired product. | 03-14-2013 |
20130071890 | Production of PHA using Biogas as Feedstock and Power Source - Methods for producing bioplastics from biogas include techniques for the production of PHB using a dirty biogas (e.g., methane from landfill, digester) as both a power source for the process and as feedstock. Biogas is split into two streams, one for energy to drive the process, another as feedstock. Advantageously, the techniques may be implemented off the power grid with no dependence upon agricultural products for feedstock. | 03-21-2013 |
20130071891 | IN SITU EXPRESSION OF LIPASE FOR ENZYMATIC PRODUCTION OF ALCOHOL ESTERS DURING FERMENTATION - Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity. | 03-21-2013 |
20130071892 | PROCESS FOR PRODUCTION OF POLYHYDROXYALKANOIC ACID USING GENETICALLY MODIFIED MICROORGANISM HAVING ENOYL-CoA HYDRATASE GENE INTRODUCED THEREIN - The present invention intends to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] with a high 3-hydroxyhexanoic acid fraction using a vegetable oil as a basic raw material. In accordance with the present invention, there is provided a method of producing a microorganism that produces poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a high 3-hydroxyhexanoic acid fraction using a vegetable oil as a basic raw material, by introducing a gene encoding R-hydratase that converts a fatty acid β-oxidation system intermediate to a monomer, (R)-3-hydroxyacyl-CoA [R-3HA-CoA], into a recombinant | 03-21-2013 |
20130109064 | MICROORGANISMS AND METHODS FOR PRODUCING 2,4-PENTADIENOATE, BUTADIENE, PROPYLENE, 1,3-BUTANEDIOL AND RELATED ALCOHOLS | 05-02-2013 |
20130109065 | SUSTAINABLE CONVERSION OF CITRUS PEEL WASTE | 05-02-2013 |
20130122559 | METHOD FOR SYNTHESIZING POLYHYDROXYALKANOATE USING A MICROORGANISM - A method for synthesizing polyhydroxyalkanoate using a microorganism includes providing a | 05-16-2013 |
20130130337 | Enzymatic Acylation Method Using an Acylphosphonate Donor - The invention relates to an enzymatic acylation method including at least the following steps of: contacting at least one compound having at least one function selected from among the amine, alcohol, or thiol functions, at least one microorganism having an acyl transfer activity and/or an acyl transfer enzyme, and at least one acylphosphonate donor of formula (I), where: R is an alkyl, alkene, uikyne, aryl, or aralkyl radical, or is —ORa, —SRa, —NRaRb, where Ra and Rb are identical or different and are H, an alkyl, alkene, alkyne, aryl or aralkyl radical, the alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; X is O or S; Y and Z, which are identical or different, are —OR1, —OR2, —SR1, —SR2, —NR′1R″1, —NR′2R″2; R1, R2, R′1, R′2, R″1 and R″2, which are identical or different, are an alkyl, alkene, alkyne, aryl or aralkyl radical, said alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; and recovering the compound including at least one acyl function, said function being selected from among the amine, alcohol, or thiol functions. | 05-23-2013 |
20130130338 | NOVEL HYDROLASE PROTEIN - It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a hydrolase protein, which consists of the amino acid sequence shown in any one of SEQ ID NOS. 2 to 5 and which has activity of catalyzing, at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO. 1, a reaction of producing (1S,2S)-1-ethoxycarbonyl-2-vinylcyclopropanecarboxylic acid from diethyl 2-vinylcyclopropane-1,1-dicarboxylate. | 05-23-2013 |
20130149755 | USE OF OXYHYDROGEN MICROORGANISMS FOR NON-PHOTOSYNTHETIC CARBON CAPTURE AND CONVERSION OF INORGANIC AND/OR C1 CARBON SOURCES INTO USEFUL ORGANIC COMPOUNDS - Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including bio-fuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO | 06-13-2013 |
20130157326 | INTEGRATED BIOPROCESSING FOR FUEL PRODUCTION - Systems and methods are provided for enhancing the integration of processes for recovering products from algae-derived biomass. The enhanced process integration allows for increased use of input streams and other reagents that are derived from renewable sources. This increases the overall renewable character of the products extracted from the algae-derived biomass. The process integration can include exchange of input streams or energy between an algae processing system and a system for processing non-algal biomass. One example of improving process integration is using oxygenates that are generated in a renewable manner as a reagent for enhancing the algae processing system. | 06-20-2013 |
20130157327 | MUTANT POLYHYDROXYALKANOIC ACID SYNTHASE GENE AND METHOD FOR PRODUCING ALIPHATIC POLYESTER USING THE SAME - A substitution mutation that improves polymerization activity of a polyhydroxyalkanoic acid synthase is identified. At least 1 amino acid residue selected from the group consisting of a histidine residue at position 17, a proline residue at position 71, a valine residue at position 131, a methionine residue at position 205, a leucine residue at position 230, and a proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from | 06-20-2013 |
20130157328 | CATALYTIC DEHYDRATION OF LACTIC ACID AND LACTIC ACID ESTERS - This invention relates to catalytic dehydration of lactic acid derived from biological fermentation and its esters into acrylic acid and acrylic acid esters respectively. Disclosed in this invention are chemical catalysts suitable for industrial scale production of acrylic acid and acrylic acid esters. This invention also provides an industrial scale integrated process technology for producing acrylic acid and acrylic acid esters from biological fermentation using renewable resources and biological catalysts. | 06-20-2013 |
20130164800 | Production of Polyhydroxyalkanoates - There is provided a process for producing polyhydroxyalkanoate (PHA) comprising the steps of culturing a biomass containing PHA-producing microbes in a culture media; and hydrolyzing said PHAs-producing microbes using microorganisms selected to release PHAs from the PHAs-containing microbes. | 06-27-2013 |
20130164801 | METHOD OF PREPARING ALKYL BUTYRATE FROM FERMENTED LIQUID USING MICROORGANISMS - Disclosed is a method of preparing alkyl butyrate, which comprises: (a) producing a fermented liquid containing butyrate salt through a fermentation process using butyric acid-producing strains; (b) obtaining an extracted liquid containing butyric acid from a continuous extracting apparatus using an extraction solvent, after converting the butyrate salt into butyric acid by adding an inorganic acid except for carbonic acid or carbon dioxide into the fermented liquid, wherein the continuous extracting apparatus includes a plurality of extraction plates which are installed inside an extraction column and perform a reciprocating motion vertically, and the broth and the extraction solvent are supplied to upper and lower portions of the column, respectively; and (c) reacting the extracted liquid and alcohol having a carbon number of 4 or less or a mixture thereof in an esterification reactor to convert a resultant product into alkyl butyrate. According to the present invention, butyric acid existing in microbic culture fluid can be efficiently extracted, and furthermore, competitively priced alkyl butyrate can be prepared without an additional process of separating the extracted solvent while minimizing energy consumption. | 06-27-2013 |
20130171703 | PRODUCING ENERGY FROM BIOLOGICAL MATERIAL - Described are methods and systems for producing synthesis gas. In some embodiments, the method includes microbiologically converting biological material to form methane and CO2; and reacting methane and CO2 formed in (a) with water and metal to form synthesis gas. Optionally, the method also includes cutting vegetation; fermenting the vegetation to form biogas comprising methane and CO2; and reacting the biogas with water and metal to form synthesis gas and metal oxide. In some embodiments of the invention, reacted metal is regenerated from metal oxide produced in the reaction. In some embodiments the regeneration comprises reacting the oxide in a bath of boiling zinc. | 07-04-2013 |
20130171704 | DICARBOXYLIC ACID PRODUCTION PROCESS - Process for producing a dicarboxylic acid comprising fermenting a microorganism in a suitable fermentation medium wherein a gas flow comprising 30% to 100% v/v oxygen as measured under atmospheric pressure is added to the fermentation medium and producing the dicarboxylic acid. | 07-04-2013 |
20130177952 | METHOD OF PRODUCING INGENOL-3-ANGELATE - The present invention relates to methods of producing ingenol-3-angelate (I) from ingenol (II). | 07-11-2013 |
20130196395 | ENZYME-CONTAINING MINI-EMULSIONS - The present invention relates to a mini-emulsion which comprises at least one hydrolase, where the continuous phase of the mini-emulsion contains at least one oxidant, while the dispersed phase comprises at least one C | 08-01-2013 |
20130203134 | METHOD FOR PRODUCING L-LACTIC ACID BY LACTIC ACID BACTERIUM UNDER PRESENCE OF PENTOSE AND CELLOOLIGOSACCHARIDES - The present invention thus provides a method for producing L-lactic acid, which comprises the step of culturing a lactic acid bacterium that can produce L-lactic acid in a medium containing any one selected from the group consisting of cellobiose, cellooligosaccharides, xylose, arabinose, and glucose derived from cellulose and/or hemicellulose as a substrate to obtain L-lactic acid. In a preferred embodiment of the present invention, | 08-08-2013 |
20130210096 | Methods and Systems for the Production of Hydrocarbon Products - Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes. | 08-15-2013 |
20130217086 | MODIFIED MICROORGANISM FOR PRODUCTION OF 1,4-BUTANEDIOL - A modified microorganism for production of 1,4-butanediol, an expression vector, and a method of producing 1,4-butanediol using the modified microorganism are provided. The method can be useful in producing 1,4-butanediol using a biological production process. | 08-22-2013 |
20130224812 | Process for Resolving Cyclopropyl Diesters - The present disclosure provides a method for the stereospecific hydrolysis of racemic 1,1-dialkyloxycarbonylcyclopropanes. | 08-29-2013 |
20130224813 | PROCESS FOR POLYLACTIC ACID PRODUCTION USING MONASCUS - A process for producing polylactic acid (PLA) comprising the steps: fermenting in a fermentation vessel a micro-organism of the genus | 08-29-2013 |
20130252292 | BIOMASS EXTRACTION PROCESS - This present invention relates to an organosolv process for the extraction of materials from lignocellulosic biomass. This invention further relates to the chemicals and their derivatives extracted from biomass, uses, apparatus, methods, and the like. In an embodiment of the invention the material extracted from the lignocellulosic biomass is levulinic acid. | 09-26-2013 |
20130273622 | Methods for the Economical Production of Biofuel from Biomass - Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel. | 10-17-2013 |
20130280772 | Reagents and Methods for Cyanobacterial Production of Bioplastics and Biomaterials - The present invention provides reagents and methods for biomaterial production from cyanobacteria. | 10-24-2013 |
20130288317 | INCREASED YIELDS OF PHAS FROM HYDROGEN FEEDING AND DIVERSE CARBON FIXATION PATHWAYS - Disclosed are methods including organisms genetically engineered to make useful products when grown on glucose as a carbon source. The organisms are genetically engineered to produce various useful products such as polyhydroxyalkanoates (PHA) monomers, polymers, and copolymers, diols, alcohols, and other useful chemicals. | 10-31-2013 |
20130288318 | HIGH OLEIC ACID OILS - The present invention relates to extracted lipid with high levels, for example 90% to 95% by weight, oleic acid. The present invention also provides genetically modified plants, particularly oilseeds such as safflower, which can used to produce the lipid. Furthermore, provided are methods for genotyping and selecting plants which can be used to produce the lipid. | 10-31-2013 |
20130309735 | PROCESS FOR PRODUCING VOLATILE ORGANIC COMPOUNDS FROM BIOMASS MATERIAL - Embodiments of the present invention provide for efficient and economical production and recovery of ethanol or other volatile organic compounds, such as acetic acid, from solid biomass material, particularly on a larger scale, such as on the commercialization or industrial scale. According to one aspect of the invention, the method comprises (a) generating at least about 10 tons of prepared biomass material by adding a microbe, optionally an acid, and optionally, an enzyme to a solid biomass; (b) storing the prepared biomass material for at least about 24 hours in a storage facility to allow production of at least one volatile organic compound from at least a portion of the sugar in the solid biomass; and (c) capturing the at least one volatile organic compound by using a solventless recovery system. | 11-21-2013 |
20130323804 | Method to Produce PHBV by Recombinant Escherichia Coli - The invention discloses a method for production of polyhydroxybutyrate-co-polyhydroxyvalerate (PHBV) by recombinant | 12-05-2013 |
20130337516 | POLYHYDROXYALKANOATE PRODUCTION METHOD - Provided are processes for the production and high efficiency processing of polyhydroxyalkanoates (PHA) from carbon sources comprising carbon-containing gases or materials. | 12-19-2013 |
20130344550 | METHODS OF BIOPLASTIC PRODUCTION - A method of producing bioplastics from algae, the method including processing algae to yield an aqueous phase containing glycerol, and fermenting the aqueous phase with a bioplastic-producing bacteria to yield bioplastics. | 12-26-2013 |
20140017743 | OXIDOREDUCTASES FOR THE STEREOSELECTIVE REDUCTION OF KETO COMPOUNDS - The invention relates to a process for the enantioselective enzymatic reduction of a keto compound to the corresponding chiral hydroxy compound, wherein the keto compound is reduced with an oxidoreductase in the presence of a cofactor, and is characterized in that an oxidoreductase is used which has an amino acid sequence in which (a) at least 70% of the amino acids are identical to the amino acids of one of the amino acid sequences SEQ ID No 1, SEQ ID No 6 and SEQ ID No 8, or (b) at least 55% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 2, or (c) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 3, or (d) at least 75% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 4, or (e) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 5, or (0 at least 50% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 7, or (g) at least 72% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 129. | 01-16-2014 |
20140017744 | METHODS OF PREPARING PARA-XYLENE FROM BIOMASS - Methods or preparing para-xylene from biomass by carrying out a Diels-Alder cycloaddition at controlled temperatures and activity ratios. Methods of preparing bio-terephthalic acid and bio-poly(ethylene terephthalate (bio-PET) are also disclosed, as well as products formed from bio-PET. | 01-16-2014 |
20140024090 | METHOD AND APPARATUS FOR CONVERTING A CARBON SOURCE INTO A HYDROCARBON OR HYDROCARBON PRECURSOR - Method for converting a carbon source such as a plant-derived material into a hydrocarbon or hydrocarbon precursor, via a microorganism-catalysed aerobic reaction. The microorganism is cultivated in the presence of the carbon source and of oxygen, in a reaction vessel having a capacity of about 2000 litres or greater which comprises an aeration system for supplying oxygen to the microorganism. Also provided is an apparatus for use in the method, comprising a reaction vessel of capacity 2000 litres or greater, and an aeration system. | 01-23-2014 |
20140024091 | USE OF OXYHYDROGEN MICROORGANISMS FOR NON-PHOTOSYNTHETIC CARBON CAPTURE AND CONVERSION OF INORGANIC AND/OR C1 CARBON SOURCES INTO USEFUL ORGANIC COMPOUNDS - Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or C1 carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing C1 chemicals including but not limited to various syngas compositions, into organic chemicals including biofuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or C1 carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO | 01-23-2014 |
20140030773 | MODIFIED MICROORGANISM FOR HIGH EFFICIENT PRODUCTION OF 1,4-BUTANEDIOL - A modified microorganism for producing a high yield of 1,4-butanediol, and a method of producing 1,4-butanediol using the modified microorganism, wherein the modified microorganism is altered so as to delete or disrupt expression of at least one of transcription regulatory factor NCg12886, GCN5-related N-acetyltransferase NCg12090, hypothetical protein NCg10224 or sugar phosphate isomerase/epimerase NCg12956. | 01-30-2014 |
20140030774 | MUTANT OF PROPIONYL-COA TRANSFERASE FROM CLOSTRIDIUM PROPIONICUM AND PREPARING METHOD FOR PLA OR PLA COPOLYMER USING THE SAME - Provided is a mutant of propionyl-CoA transferase from | 01-30-2014 |
20140030775 | RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE [LACTATE-CO-GLYCOLATE] COPOLYMER FROM GLUCOSE, AND METHOD FOR PREPARING [LACTATE-CO-GLYCOLATE] COPOLYMER USING SAME - There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment. | 01-30-2014 |
20140038249 | Integrated Process For The Production Of Jatropha Methyl Ester And By Products - The present invention provides a simpler and more energy efficient process for the preparation of fatty acid methyl ester (biodiesel) from sun dried whole seed capsules of | 02-06-2014 |
20140057326 | MICROORGANISM PRODUCING HIGH-MOLECULAR-WEIGHT PHA - The present invention provides a microorganism that synthesizes a high-molecular-weight PHA, and a method for producing a high-molecular-weight PHA, which have a productivity of at least 100 g/L. The provision is achieved by controlling the specific activity of a PHA synthase in cells of a microorganism that belongs to the genus | 02-27-2014 |
20140073022 | PRODUCTION OF POLYHYDROXYALKANOATES WITH A DEFINED COMPOSITION FROM AN UNRELATED CARBON SOURCE - Cells and methods for producing polyhydroxyalkanoates. The cells comprise one or more recombinant genes selected from an R-specific enoyl-CoA hydratase gene, a PHA polymerase gene, a thioesterase gene, and an acyl-CoA-synthetase gene. The cells further have one or more genes functionally deleted. The functionally deleted genes include such genes as an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase, and a 3-ketoacyl-CoA thiolase gene. The recombinant cells are capable of using producing polyhydroxyalkanoates with a high proportion of monomers having the same carbon length from non-lipid substrates, such as carbohydrates. | 03-13-2014 |
20140141478 | PROCESS FOR REACTING A CARBOXYLIC ACID ESTER - The invention provides a process for reacting a carboxylic acid ester of the formula (I) | 05-22-2014 |
20140147898 | Separation Process - A process for treating a mixture of R,R- and S,S-lactide is provided. The process involves contacting the mixture with an aliphatic alcohol and an enzyme to produce a mixture comprising aliphatic ester of lactic acid corresponding to one lactide enantiomer and the aliphatic ester of lactyllactic acid corresponding to the other lactide enantiomer; separating the mixture from the enzyme, and recycling the enzyme to the process; and separating the aliphatic ester of lactic acid from the aliphatic ester of lactyl-lactic acid by fractional distillation. Also provided are processes for the production of S-lactic acid, S,S-lactide, poly-S-lactic acid, R-lactic acid, R,R-lactide, poly-R-lactic acid and stereocomplex polylactic acid. | 05-29-2014 |
20140154756 | PROCESSES AND APPARATUS FOR PRODUCING NANOCELLULOSE, AND COMPOSITIONS AND PRODUCTS PRODUCED THEREFROM - Processes disclosed are capable of converting biomass into high-crystallinity nanocellulose with surprisingly low mechanical energy input. In some variations, the process includes fractionating biomass with an acid (such as sulfur dioxide), a solvent (such as ethanol), and water, to generate cellulose-rich solids and a liquid containing hemicellulose and lignin; and mechanically treating the cellulose-rich solids to form nanofibrils and/or nanocrystals. The total mechanical energy may be less than 500 kilowatt-hours per ton. The crystallinity of the nanocellulose material may be 80% or higher, translating into good reinforcing properties for composites. The nanocellulose material may include nanofibrillated cellulose, nanocrystalline cellulose, or both. In some embodiments, the nanocellulose material is hydrophobic via deposition of some lignin onto the cellulose surface. Optionally, sugars derived from amorphous cellulose and hemicellulose may be separately fermented, such as to monomers for various polymers. These polymers may be combined with the nanocellulose to form completely renewable composites. | 06-05-2014 |
20140154757 | PROCESSES AND APPARATUS FOR PRODUCING NANOCELLULOSE, AND COMPOSITIONS AND PRODUCTS PRODUCED THEREFROM - Processes disclosed are capable of converting biomass into high-crystallinity nanocellulose with surprisingly low mechanical energy input. In some variations, the process includes fractionating biomass with an acid (such as sulfur dioxide), a solvent (such as ethanol), and water, to generate cellulose-rich solids and a liquid containing hemicellulose and lignin; and mechanically treating the cellulose-rich solids to form nanofibrils and/or nanocrystals. The total mechanical energy may be less than 500 kilowatt-hours per ton. The crystallinity of the nanocellulose material may be 80% or higher, translating into good reinforcing properties for composites. The nanocellulose material may include nanofibrillated cellulose, nanocrystalline cellulose, or both. In some embodiments, the nanocellulose material is hydrophobic via deposition of some lignin onto the cellulose surface. Optionally, sugars derived from amorphous cellulose and hemicellulose may be separately fermented, such as to monomers for various polymers. These polymers may be combined with the nanocellulose to form completely renewable composites. | 06-05-2014 |
20140154758 | BIOMASS DERIVED METHYL METHACRYLATE AND CORRESPONDING MANUFACURING METHOD, USES AND POLYMERS - The invention relates to methyl methacrylate characterized in that at least one portion of the carbons thereof is biologically sourced and, more specifically, in that it contains between 0.2×10 | 06-05-2014 |
20140170717 | Ester Synthesis - The present invention relates to a process for preparing esters from fatty alcohols, in which fatty alcohols and fatty acids are reacted in the presence of an enzyme at a temperature in the range of 30 to 50° C., the water which forms is removed and the reaction is completed under reduced pressure at a temperature of 50 to 80° C. | 06-19-2014 |
20140186910 | DHAD Variants for Butanol Production - Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol. | 07-03-2014 |
20140193868 | EXTRACELLULAR POLYHYDROXYALKANOATES PRODUCED BY GENETICALLY ENGINEERED MICROORGANISMS - The present invention is in the field of biosynthesis of polyhydroxyalkanoates (PHA). The invention relates to a genetically engineered microorganism having at least one gene involved in the metabolism, preferably in the production, of polyhydroxyalkanoates (PHA). This microorganism is useful in commercial production of polyhydroxyalkanoates. The present invention further relates to a method for the production of polyhydroxyalkanoates (PHA). | 07-10-2014 |
20140206048 | COMPOSITIONS AND METHODS FOR BIOFERMENTATION OF OIL-CONTAINING FEEDSTOCKS - The present invention provides compositions and methods for the use of oil-containing materials as feedstocks for the production the bioproducts by biofermentation. In one preferred embodiment, surfactants are not used in compositions and the methods of the invention. In one preferred embodiment the oil-containing feedstocks are the by-products of other industrial processes including microbial, plant and animal oil processing. | 07-24-2014 |
20140206049 | METHOD FOR PRODUCING POLYHYDROXYALKANOIC ACID - Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production. | 07-24-2014 |
20140234921 | Novel cutinases, their production and uses - The present invention relates to novel polypeptides, or fragments of polypeptides, genes encoding them and means for producing said polypeptides. In detail the invention relates to polypeptides having esterase, suberinase and/or cutinase activity at low pH. This invention relates also to compositions containing the polypeptides and methods of using the polypeptides. | 08-21-2014 |
20140234922 | SYSTEM AND METHOD FOR THE PRODUCTION OF ALKYL ESTERS - A method of producing alkyl esters including processing a high free fatty acid feedstock including a mixture of triglycerides and free fatty acids to remove water and solids to create a processed feedstock. The processed feedstock is introduced and mixed into a reaction vessel, the reaction vessel includes water, at least one enzyme, and alcohol. The reacted contents are separated into a glycerin phase and an alkyl ester phase. The alkyl ester phase is treated with a primary alcohol and a flocculant. | 08-21-2014 |
20140242650 | Recominant Ralstonia Eutropha Capable of Producing Polyactic and Acid or Polylatic Acid Polymer, and Method for Producing Polyactic Acid or Polylatic Acid Copolymer Using the Same - Provided are a recombinant | 08-28-2014 |
20140242651 | ENZYMATICALLY CATALYZED METHOD OF PREPARING MONO-ACYLATED POLYOLS - The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacylglycerol lipase mutants, as for example derived from | 08-28-2014 |
20140242652 | METHOD FOR PRODUCING SUCCINIC ACID - A method for producing succinic acid comprising (A) a step of converting an organic raw material to succinic acid in the presence of a microorganism or a processed product thereof in an aqueous medium and (B) a step of recovering the succinic acid, the method being characterized in that (A) the step of converting an organic raw material to succinic acid comprises:
| 08-28-2014 |
20140273115 | SYSTEM AND METHOD FOR CONTROLLING METABOLITE PRODUCTION IN A MICROBIAL FERMENTATION - A method is provided for controlling a metabolic profile of an anaerobic microbial fermentation culture. In particular, a metabolic profile of a fermentation process is controlled by controlling the amount of dissolved CO2 provided to a culture. Further provided is a method of producing one or more products by microbial fermentation of a gaseous substrate through feeding tail gas CO2 from a reactor to a second reactor, or by recycling tail gas CO2 to the same reactor. | 09-18-2014 |
20140295507 | METHOD FOR PRODUCING BIODEGRADABLE POLYMER AND BIOMASS FUEL CONVERTED FROM CARBON SOURCE BY RECOMBINANT MICROORGANISMS - Disclosed is a method for producing biodegradable polymer and ethanol converted from carbon source by using recombinant microorganisms, comprising the steps of: (A) providing recombinant microorganisms transformed with plasmids containing at least a gene encoding for glycerol utilizing enzyme and a gene encoding for polyhydroxyalkanoate synthase; (B) culturing the recombinant microorganisms in a medium containing glycerol; (C) inducing expression of the genes of step (A), thereby obtaining polyhydroxyalkanoate and ethanol; and (D) recovering the polyhydroxyalkanoate and the ethanol; wherein the recombinant microorganisms have a glycerol utilization rate more than 90% (w/w), and have polyhydroxyalkanoate accumulated therein to a biomass content thereof at least 30% (w/w). | 10-02-2014 |
20140302571 | PROCESSING BIOMASS - Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol. | 10-09-2014 |
20140302572 | METHOD FOR PRODUCING POLYHYDROXYALKANOATE HAVING LONG MAIN CHAIN STRUCTURE - An object of the present invention is to provide a polyhydroxyalkanoate which forms a useful bioplastic or biomaterial. Specifically, the present invention relates to a method for producing a polyhydroxyalkanoate that includes at least a 5-hydroxyvalerate unit, in which the method for producing a polyhydroxyalkanoate includes culturing a microorganism belonging to | 10-09-2014 |
20140308719 | PROCESS FOR PRODUCING (R)-3-HYDROXYBUTYL (R)-3-HYDROXYBUTYRATE - Embodiments of the present invention are directed to processes for the production of (R)-3-hydroxybutyl (R)-3-hydroxybyrate. Poly (R)-3-hydroxybyrate is transesterified with an alcohol, to form a first ester portion and a second ester portion. The first ester portion is reduced to the diol to form a diol portion and the diol portion is reacted with the second ester portion to produce (R)-3-hydroxybutyl (R)-3-hydroxybyrate. | 10-16-2014 |
20140342413 | Separation Process - A process for treating a mixture of R,R- and S,S-lactide is provided. The process involves contacting the lactide mixture with an aliphatic alcohol and/or an alkoxide to produce a mixture of R,R- and S,S-aliphatic ester of lactyllactic acid, subsequently contacting the mixture of R,R- and S,S-aliphatic ester of lactyllactic acid with an enzyme to produce a mixture comprising aliphatic ester of lactic acid corresponding to one lactide enantiomer and the aliphatic ester of lactyllactic acid corresponding to the other lactide enantiomer, and recovering the product. Also provided are processes for the production of S-lactic acid, S,S-lactide, poly-S-lactic acid, R-lactic acid, R,R-lactide, poly-R-lactic acid and stereocomplex polylactic acid. | 11-20-2014 |
20140349353 | ENGINEERED STRAIN OF ESCHERICHIA COLI FOR PRODUCTION OF POLY-R-3-HYDROXYALKANOATE POLYMERS WITH DEFINED MONOMER UNIT COMPOSITION AND METHODS BASED THEREON - Methods and systems for producing prescribed unit size poly(3-hydroxyalkanoate) (PHA) polymers and copolymers are provided. The methods and systems can employ recombinant bacteria that are not native producers of PHA or lack enzymes to degrade PHA once synthesized, metabolize short to long chain fatty acids without induction, and express an (R)-specific enoyl-CoA hydratase and a PHA synthase, the (R)-specific enoyl-CoA hydratase and PHA synthase having wide substrate specificities. The recombinant bacteria are fed at least one fatty acid substrate that is equal in carbon length to the prescribed or desired unit size of the PHA polymer to be produced. The prescribed unit size PHA that is produced is then isolated and/or purified. | 11-27-2014 |
20140377819 | PROCESS FOR PRODUCING MICROBIAL COPOLYESTERS FROM SUCROSE-CONTAINING FEEDSTOCKS - A process for producing hydroxyalkanoate copolymers, which comprises: (i) pre-treating a sucrose-containing feedstock in an acidic solution; (ii) feeding the pre-treated feedstock into a bioreactor containing polyhydroxyalkanoate producing microbial cells; (iii) cultivating the polyhydroxyalkanoate producing microbial cells to form a cell mass containing the hydroxyalkanoate copolymers; (iv) recoverying the hydroxyalkanoate copolymers from the cell mass. The pre-treating step has the main function of hydrolyzing sucrose into glucose and fructose, which in turn are converted into 4-ketovaleric acid to give a mixture of mono-saccharides and organic precursors for microbial synthesis of hydroxyalkanoate copolymers, and particularly of PHBVV ter-polymers. Complex and expensive purification processes of the substrates obtained from the pre-treating step are not needed. The solutions can be directly used as the feeding solutions for microbial PHA biosynthesis. | 12-25-2014 |
20150010967 | PROCESSING BIOMASS - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material. | 01-08-2015 |
20150024443 | PROCESS FOR THE PREPARATION OF INGENOL-3-ANGELATE - The invention relates to methods for preparing ingenol-3-angelate from ingenol or ingenol derivatives. | 01-22-2015 |
20150031098 | MUTANT OF PROPIONYL-COA TRANSFERASE FROM CLOSTRIDIUM PROPIONICUM AND PREPARING METHOD FOR PLA OR PLA COPOLYMER USING THE SAME - Provided is a mutant of propionyl-CoA transferase from | 01-29-2015 |
20150056673 | RECOMBINANT MICROORGANISM - The invention concerns a microorganism which is genetically modified so as to i) synthesize a hydrocarbon monomer by fermentation of a carbon source, and ii) depolymerize a polymer constituted at least by hydrocarbon monomer which it is capable of synthesizing. The invention also concerns a method for producing a hydrocarbon monomer using a genetically modified microorganism of this type, as well as the coculture of this microorganism with another microorganism which is capable of synthesizing a polymer of interest. | 02-26-2015 |
20150064750 | MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF AROMATICS, 2,4-PENTADIENOATE AND 1,3-BUTADIENE - The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene. | 03-05-2015 |
20150064751 | METHODS FOR CONTROL OF FLUX IN METABOLIC PATHWAYS THROUGH PROTEASE MANIPULATION - The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage. | 03-05-2015 |
20150072384 | Genetically Modified Organisms for Increased Microbial Production of 3-Hydroxypropionic Acid Involving an Oxaloacetate alpha-decarboxylase - Microorganism compositions are described that comprise combinations of genetic modifications that include a genetic modification to increase oxaloacetate alpha-decarboxylase enzymatic activity. By such genetic modification a 3-hydroxypropionic acid (“3-HP”) production pathway is provided or improved. In various embodiments, comprising other genetic modifications, including selected gene disruptions, 3-HP production is greater than in a control microorganism lacking such combinations of genetic modifications | 03-12-2015 |
20150072385 | POLYHYDROXYALKANOATE PRODUCTION METHODS AND SYSTEMS FOR SAME - Several embodiments of the invention relate generally to a system and methods for the treatment of gaseous emissions comprising methane and one or more non-methane compounds that can influence the metabolism of methane-oxidizing microorganisms. In several embodiments, there is provided a system and methods for the treatment of methane emissions through the use of methanotrophic microorganisms to generate functionally consistent and harvestable products. Certain embodiments of the invention are particularly advantageous because they reduce environmentally-destructive methane emissions and produce harvestable end-products. | 03-12-2015 |
20150079646 | GENETICALLY ENGINEERED YEAST CELLS - The present invention relates to yeast cells producing high levels of acetoacetyl-CoA. It also relates to a method for making such yeast cells and to the use of such yeast cells in a method for producing acetyl-CoA derived products. | 03-19-2015 |
20150087034 | METHOD FOR PRODUCING POLYBUTYLENE TEREPHTHALATE - The present invention aims to provide a method for producing polybutylene terephthalate (PBT) with an excellent color using biomass-derived 1,4-butanediol (BG). The invention relates to a method for producing PBT comprising a step of subjecting a diol component containing raw material 1,4-BG having a nitrogen content of 0.01 to 50 ppm by mass and a dicarboxylic acid component to esterification or ester-exchange reaction, and a polycondensation reaction step for obtaining PBT from the reactant, wherein the content of gamma butyrolactone in the raw material 1,4-BG is 1 to 100 ppm by mass. | 03-26-2015 |
20150140620 | BIOSYNTHETIC PATHWAYS, RECOMBINANT CELLS, AND METHODS - This disclosure describes engineered biosynthetic pathways, recombinant cells, and methods relating to biosynthesis of esters. The recombinant cells may be modified to exhibit increased biosynthesis of an ester compared to a wild-type control. The recombinant cell may be incubated in medium that includes a carbon source under conditions effective for the recombinant cell to produce an ester. This disclosure also describes a method that generally includes introducing into a host cell a heterologous polynucleotide encoding at least one polypeptide that catalyzes a step in converting a carbon source to an ester, wherein the at least one polynucleotide is operably linked to a promoter so that the modified host cell catalyzes conversion of the carbon source to an ester. | 05-21-2015 |
20150140621 | POLYHYDROXYALKANOATE PRODUCTION AND RELATED PROCESSES - Embodiments of the invention relate generally to processes for the production and processing of polyhydroxyalkanoates (PHA) from carbon sources. In several embodiments, PHAs are produced at high efficiencies from carbon-containing gases through the utilization of a regenerative polymerization system. | 05-21-2015 |
20150140622 | Reagents and Methods for Cyanobacterial Production of Bioplastics and Biomaterials - The present invention provides reagents and methods for biomaterial production from cyanobacteria. | 05-21-2015 |
20150140623 | Synthesis of Diester-Based Lubricants from Enzymatically-Directed Epoxides - The present invention is generally directed to methods of making diester-based lubricant compositions, wherein formation of diester species proceeds via esterification of epoxide intermediates, and wherein the epoxide intermediates are generated via an enzymatically-driven mechanism. In some embodiments, the methods for making such diester-based lubricants utilize a biomass precursor and/or low value (e.g., Fischer-Tropsch (FT) olefins and/or alcohols) so as to produce high value diester-based lubricants. In some embodiments, such diester-based lubricants are derived from FT olefins and fatty acids. The fatty acids can be from a bio-based source (i.e., biomass, renewable source) or can be derived from FT alcohols via oxidation. | 05-21-2015 |
20150322359 | METHOD FOR PREPARING BIODIESEL - Disclosed herein is a method of preparing biodiesel, including the steps of: removing foreign materials from biodiesel raw oil containing lower fatty acid and having an acid value of 20 mgKOH/g or less with an adsorbent; dewatering the foreign materials removed biodiesel raw oil; passing the dewatered biodiesel raw oil through a solid acid catalyst reaction guard bed provided with a cation exchange resin for removing metal cations at 20˜80° C. at a flow rate of 6 vol % (6 SV) or less, mixing methanol with the passed biodiesel raw oil, and then passing the mixture through a solid acid catalyst reaction main bed provided with a solid acid catalyst for an esterification reaction at 70˜120° C. at a flow rate of 0.5˜1.5 SV to adjust a final acid value of the biodiesel raw oil to 5 mgKOH/g or less; and reacting the biodiesel raw oil having the final acid value of 5 mgKOH/g or less in the presence of an alkali catalyst. | 11-12-2015 |
20150337344 | METHOD FOR PRODUCING POLYHYDROXYALKANOATES BY MICROORGANISMS - Method for simultaneous selection and maintenance of a selected microbial consortium for polyhydroxyalkanoates (PHA) production in only one step, said microbial consortium being fed with at least one readily biodegradable carbon substrate, comprising a first step of aerobic cultivation of said microbial consortium in a mixed biological reactor. According to the invention, the specific cell growth rate μl of said microbial consortium is fixed at a target value, in said first step of aerobic cultivation and in that said first step of aerobic cultivation is performed under nutrient limitation such as the readily biodegradable carbon substrate uptake rate qS | 11-26-2015 |
20150344916 | METHOD FOR PRODUCING 3-HYDROXYPROPIONIC ACID AND OTHER PRODUCTS - This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid. | 12-03-2015 |
20150353967 | METHOD FOR INCREASED PRODUCTIVITY OF POLYHYDROXYALKANOATES (PHAS) IN FED-BATCH PROCESSES FOR BIOMASS DERIVED FROM THE TREATMENT OF WASTEWATER - This disclosure relates to methods for producing a PHA-rich-biomass from open mixed cultures. Mixed liquor containing biomass is directed into a fed-batch reactor. The reactor includes at least one biomass stimulating zone and at least one biomass maintenance zone. A feed containing RBCOD, bioavailable N, and bioavailable P is directed into the fed-batch reactor. The respiration rate of at least a portion of the biomass is intermittently and repeatedly stimulated in the stimulating zone by directing the feed into the fed-batch reactor and exposing the biomass to a relatively high concentration of RBCOD. The biomass is then transferred to the maintenance zone, where the biomass is exposed to a relatively low concentration of RBCOD. Thereafter, the biomass is circulated back and forth between the stimulating zone and the maintenance zone. Throughout the methods, the concentration of N and/or P relative to the RBCOD in the feed is controlled by controlling the ratio of N to RBCOD and/or the ration of P to RBCOD in the feed. | 12-10-2015 |
20150368306 | ACID-RESISTANT YEAST CELL WITH REDUCED FPS1 ACTIVITY AND METHOD OF PRODUCING LACTATE BY USING THE YEAST CELL - Provided are an acid-resistant yeast cell with genetic modification and reduced Fps1 activity compared to that of a parent cell without the genetic modification; and a method of producing lactate by using the yeast cell. | 12-24-2015 |
20150376107 | METHOD FOR PREPARING METHYL LACTATE - The invention pertains to a process for preparing methyl lactate inluding the steps of: bringing an aqueous liquid comprising lactic acid, methanol, and at least 5 wt. % of a dissolved chloride salt selected from magnesium chloride, calcium chloride, and zinc chloride to reaction conditions, thereby obtaining methyl lactate, wherein an extractant is provided to the reaction mixture before, during, and/or after formation of methyl lactate; subjecting the reaction mixture to a liquid-liquid separation step wherein an organic phase comprising methyl lactate and extractant is separated from an aqueous phase comprising dissolved chloride salt. The extractant preferably includes one or more compounds selected from C5+ ketones and C3-C10 ethers, in particular C5-C8 ketones, more in particular methyl isobutyl ketone. It has been found that the process according to the invention makes it possible to manufacture methyl lactate efficiently and in high yield. | 12-31-2015 |
20160010124 | MICROORGANISM HAVING NOVEL ACRYLIC ACID SYNTHESIS PATHWAY HAVING ENHANCED ACTIVITY OF COA ACYLATING ALDEHYDE DEHYDROGENASE AND METHOD OF PRODUCING ACRYLIC ACID USING THE SAME | 01-14-2016 |
20160024603 | NOVEL EXTREME THERMOPHILIC BACTERIA OF THE GENUS CALDICELLULOSIRUPTOR - A method for converting lignocellulosic biomass material to a carboxylic acid comprising the step of contacting the lignocellulosic biomass material with a microbial culture for a period of time at an initial temperature and an initial pH, thereby producing an amount of a carboxylic acid; wherein the microbial culture comprises an extremely thermophilic bacteria strain selected from the group consisting of | 01-28-2016 |
20160032330 | P-450-CATALYZED ENANTIOSELECTIVE CYCLOPROPANATION OF ELECTRON-DEFICIENT OLEFINS - The present invention pertains to the use of engineered variants of enzyme CYP102A, also known as P450-BM3, for cyclopropanation of olefins containing electron-withdrawing groups. One exemplary enzyme variant, referred to as BM3-HStar, contains five mutations away from wild-type P450-BM3, and demonstrates high activity towards cyclopropanation of olefinic substrates using ethyldiazoacetate (EDA) and other carbene transfer reagents. Products of these reactions are potential precursors of levomilnacipran derivatives, a class of compounds that have been shown to be selective inhibitors of monoamine transporters. In addition, cyclopropanation reactions with the P450-BM3 enzyme variants of the invention can be conducted in whole cells expressing the enzyme variants and can proceed under aerobic conditions. | 02-04-2016 |
20160040197 | PRODUCTION METHOD FOR COPOLYMER POLYHYDROXYALKANOATE USING GENETICALLY MODIFIED STRAIN OF FATTY ACID -OXIDATION PATHWAY - An object of the present invention is to provide a method for producing poly(3-hydroxybutyrate-co-3-hydroxyhexanoate (P(3HB-co-3HHx)) having a high 3-hydroxyhexanoate fraction using a vegetable oil as a basic raw material. According to the present invention, a method is provided for producing P(3HB-co-3HHx) having a high 3-HHx fraction using a vegetable oil as a basic raw material by disrupting and so forth at least one gene encoding 2-enoyl-CoA hydratase or at least one gene encoding 3-hydroxyacyl-CoA dehydrogenase on a chromosome of a recombinant | 02-11-2016 |
20160060661 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation. | 03-03-2016 |
20160068875 | Production of tailored PHA copolymers from natural gas - A method of producing polyhydroxyalkanoic acid (PHA)-producing biomass is provided that includes obtaining a methane-oxidizing inoculum, flushing the methane-oxidizing inoculum with natural gas and oxygen, amending the flushed methane-oxidizing inoculum with a fresh growth medium, using a non-aseptic bioreactor for growing a PHA-producing biomass, where the non-aseptic bioreactor is seeded with the amended methane-oxidizing inoculum, where a natural gas and oxygen mixture is added to the non-aseptic bioreactor, where a growth medium comprising ammonium and nutrients required for exponential growth is added to the non-aseptic bioreactor, harvesting a portion of the methane-oxidizing biomass and incubating the harvested portion in the absence of nitrogen and with the natural gas and oxygen mixture, where a PHA-enriched biomass is produced, purifying PHA from the PHA-enriched biomass, and adding the fresh growth medium and the natural gas and oxygen mixture to the bioreactor to re-grow the methane-oxidizing inoculum. | 03-10-2016 |
20160076064 | ENGINEERED STRAIN OF ESCHERICHIA COLI FOR PRODUCTION OF POLY-R-3-HYDROXYALKANOATE POLYMERS WITH DEFINED MONOMER UNIT COMPOSITION AND METHODS BASED THEREON - Methods and systems for producing prescribed unit size azido-poly(3-hydroxyalkanoate) (azido-PHA) polymers and copolymers are provided. The methods and systems can employ recombinant bacteria that are not native producers of PHA or lack enzymes to degrade PHA once synthesized, metabolize short to long chain fatty acids without induction, and express an (R)-specific enoyl-CoA hydratase and a PHA synthase, the (R)-specific enoyl-CoA hydratase and PHA synthase having wide substrate specificities. The recombinant bacteria are fed at least one ω-azidofatty acid substrate that is equal in carbon length to the prescribed or desired unit size of an azido-PHA polymer to be produced. Azido-PHA polymers or azido-PHA co-polymers can be conjugated via copper-catalyzed alkyne-azide cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) reactions. The prescribed unit size conjugated azido-PHA polymer or orthogonally conjugated azido-PHA co-polymer that is produced is then isolated and/or purified. | 03-17-2016 |
20160102325 | Method for Storing Gaseous Hydrogen Through Producing Methanoate (Formate) - The present invention relates to a method for storing gaseous hydrogen, comprising the steps of producing methanoate (formate) through contacting gaseous hydrogen with carbon dioxide in the presence of a hydrogen dependent carbondioxide reductase (HDCR), and thereby storing of said gaseous hydrogen. The HDCR and/or its complex is preferably derived from | 04-14-2016 |
20160102326 | METHOD FOR PRODUCING POLY-3-HYDROXYALKANOIC ACID AND AGGLOMERATES THEREOF - When industrially separating and purifying poly-3-hydroxyalkanoic acid produced by a microorganism, to obtain poly-3-hydroxyalkanoic acid agglomerates having an arbitrary volume mean particle diameter with favorable productivity and with decreased amount of an organic solvent used is enabled while decreasing contaminants derived from constitutive components of cellular bodies. According to the present invention, agglomerates of poly-3-hydroxyalkanoic acid are obtained by adjusting the pH of an aqueous poly-3-hydroxyalkanoic acid suspension to fall within an acidic region. | 04-14-2016 |
20160108442 | MICROORGANISMS AND METHODS FOR PRODUCING (3R)-HYDROXYBUTYL (3R)-HYDROXYBUTYRATE - Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention. | 04-21-2016 |
20160145183 | OPTIMIZED PROCESS FOR EXTRACTION OF FERULIC ACID WITH PRETREATMENT - A process for extraction of ferulic acid present in an aqueous phase, obtained by treatment of at least one plant material, and also containing polysaccharides, is described, said process comprising at least the following steps:
| 05-26-2016 |
20160145655 | POLYHYDROXYALKANOIC ACID COMPOSITIONS AND METHODS FOR GENERATING SAME - Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production. | 05-26-2016 |
20160168602 | METHOD FOR THE ENZYME-CATALYSED HYDROLYSIS OF POLYACRYLIC ACID ESTERS, AND ESTERASES USED THEREFOR | 06-16-2016 |
20190144892 | INTEGRATED MIXOTROPHIC FERMENTATION METHOD | 05-16-2019 |
20220136016 | MODIFIED CHLORAMPHENICOL ACETYLTRANSFERASE AND BIOSYNTHESIS METHOD OF MAKING ESTERS USING SAME - A modified chloramphenicol acetyltransferase comprising a tyrosine residue 20 having a phenylalanine (Y20F) mutation, a microorganism harboring the modified chloramphenicol acetyltransferase, and a method of producing ester by feeding the microorganism are disclosed. The method includes providing the microorganism harboring a modified chloramphenicol acetyltransferase in an environment suitable for the microorganism to produce an ester and feeding the microorganism (i) a sugar or a cellulose, and (ii) an alcohol and/or a carboxylic acid. | 05-05-2022 |