Entries |
Document | Title | Date |
20080199923 | Process for the Heterotrophic Production of Microbial Products with High Concentrations of Omega-3 Highly Unsaturated Fatty Acids - Disclosed is a process for growing the microflora | 08-21-2008 |
20080220486 | Method for growing photosynthetic organisms - A method of growing photosynthetic organisms comprising providing the organisms with flue gases from a fossil-fuel power plant, the gases being previously treated by desulfurization. The carbon dioxide (CO | 09-11-2008 |
20080227165 | Process for producing conjugated fatty acid and food/drink produced by the process - A conjugated fatty acid such as the cis-9, trans-11 isomer of conjugated linoleic acid having high bioactivities is selectively and efficiently produced by conjugating an unsaturated fatty acid having at least two double bonds with the use of viral cells, dead cells or a cell extract of at least one bacterium selected from the group consisting of | 09-18-2008 |
20080241897 | Enzymatic Oil Interesterification - An improved process for enzymatic interesterification of oil containing a chelating agent with a base and a lipase. | 10-02-2008 |
20080254521 | DELTA-8 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ8 desaturase genes, which have the ability to convert eicosadienoic acid (EDA; 20:2 ω-6) to dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) and/or eicosatrienoic acid (ETrA; 20:3 ω-3) to eicosatetraenoic acid (ETA; (20:3 ω-3). Isolated nucleic acid fragments and recombinant DNA constructs comprising such fragments encoding Δ8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ8 desaturases in oleaginous yeast are disclosed. | 10-16-2008 |
20080254522 | DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid (LA; 18:2 ω-6) to eicosadienoic acid (EDA; 20:2 ω-6) and/or α-linolenic acid (ALA; 18:3 ω-3) to eicosatrienoic acid (ETrA; 20:3 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in oleaginous yeast are disclosed. | 10-16-2008 |
20080274521 | DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ5 desaturases in oleaginous yeast are disclosed. | 11-06-2008 |
20090011480 | Use of Cellulosic Materials for Cultivation of Microorganisms - The invention provides methods of cultivating oil-bearing microbes using cellulosic material. Also provided are microorganisms that manufacture non-alcohol-based fuels and fuel feedstocks through a process of converting cellulosic materials into oils. Also provided are compositions comprising depolymerized cellulosic materials and oil-bearing microbes. Some methods of microbial fermentation are provided that comprise combining depolymerized cellulosic materials with other non-cellulosic feedstocks to enhance the economics of renewable fuel manufacturing. Particular advantages of the processes provided herein include production of oils rather than alcohols through cellulosic processes. Additional advantages include methods for manufacturing high nutrition oils from non-edible feedstocks such as wood chips, switchgrass, and bagasse. | 01-08-2009 |
20090029427 | Increased Yield in Gas-to-Liquids Processing Via Conversion of Carbon Dioxide to Diesel Via Microalge - The present invention is generally directed to systems and methods for integrating gas-to-liquids (GTL) processing with biofuels production. In some embodiments of the present invention, carbon dioxide (CO | 01-29-2009 |
20090029428 | Novel microorganism, lipid-modifying agent, and the method of manufacturing 2-acyl lysophospholipids - The present invention provides a new supply source of phospholipase A | 01-29-2009 |
20090042263 | Immobilized Enzymes and Methods of Using Thereof - The disclosed matter relates to immobilized enzymes and methods of use thereof. | 02-12-2009 |
20090061492 | Method for producing biodiesel - The present invention relates to the production of biodiesel. In particular, the present invention provides systems and methods for fermenting biomass materials with transgenic plant materials expressing the WRI1 transcription factor. In preferred embodiments, WRI1 is expressed in canola. The transgenic canola plants are fermented with a biomass source so that oil is produced using carbohydrates from the biomass source as an energy source. | 03-05-2009 |
20090075349 | METHOD OF RECOVERING LIPASE ACTIVITY - The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived from | 03-19-2009 |
20090093033 | PUFA-PKS Genes From Ulkenia - The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms. | 04-09-2009 |
20090098622 | SCHIZOCHYTRIUM PKS GENES - The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of | 04-16-2009 |
20090104673 | Process for Producing Triglycerides - A process for producing triglycerides comprises: (a) subjecting a first triglyceride comprising at least 40% by moles of oleic acid residues, based on total acyl groups in the triglyceride, to a reaction with stearic acid, at least one ester of stearic acid or a mixture thereof, to obtain a composition comprising 1,3-distearoyl 2-oleoyl glyceride and trioleoyl glyceride; (b) treating the composition to form a first fraction having an increased amount by weight of oleoyl groups compared to said composition and a second fraction having an increased amount by weight of stearoyl groups compared to said composition; (c) hydrolysing the first fraction to form oleic acid; and (d) reacting said oleic acid or an ester thereof with a triglyceride comprising at least 50% by moles of palmitic acid residues, based on total acyl groups, to form a composition comprising 1,3-dioleoyl 2-palmitoyl glyceride. | 04-23-2009 |
20090104674 | Diacylglycerol Acyltransferases for Alteration of Polyunsaturated Fatty Acids and Oil Content in Oleaginous Organisms - Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., | 04-23-2009 |
20090117629 | ISOPRENOID WAX ESTER SYNTHASES, ISOPRENOID ACYL COA-SYNTHETASES, AND USES THEREOF - The present invention provides isolated polynucleotides and isolated polypeptides. The polypeptides of the present invention have isoprenoid wax ester synthase activity or isoprenoid acyl CoA-synthetase activity. The present invention also includes methods of using the polynucleotides and polypeptides of the present invention. For instance, the methods include producing biodiesel and producing wax esters. | 05-07-2009 |
20090123982 | Triglyceride Process - A process for producing triglycerides which comprises: (a) subjecting a first triglyceride comprising at least 40% by moles of oleic acid residues, based on total acyl groups in the triglyceride, to an alcoholysis reaction with an alcohol having from 1 to 6 carbon atoms to obtain a composition comprising 2-oleoyl monoglyceride and at least one acyl ester of said alcohol; (b) reacting the 2-oleoyl monoglyceride with an acylating agent comprising at least one C12 to C24 saturated fatty acid, at least one ester of said fatty acid or a mixture thereof, to obtain a 1,3-saturated fatty acid acyl 2-oleoyl glyceride; and (c) separating at least a part of the at least one acyl ester after or during step (a) or step (b); (d) reacting the acyl ester with a second glyceride to form 1,3-dioleoyl 2-palmitoyl glyceride. | 05-14-2009 |
20090130728 | Process for Producing a Triglyceride - A process for the production of a composition comprising 1,3-dioleyl-2-palmitoyl glyceride (OPO) comprises subjecting a palm oil stearin, with an iodine value (IV) between about 2 and about 12 to enzymic transesterification, with oleic acid or a non-glyceride ester thereof. | 05-21-2009 |
20090137013 | MICROORGANISMS AND METHODS FOR INCREASED HYDROGEN PRODUCTION USING DIVERSE CARBONACEOUS FEEDSTOCK AND HIGHLY ABSORPTIVE MATERIALS - The disclosed invention relates to an isolated hydrogen gas producing microorganism, termed | 05-28-2009 |
20090142816 | GLIOCLADIUM ISOLATE C-13 AND METHODS OF ITS USE FOR PRODUCING VOLATILE COMPOUNDS AND HYDROCARBONS - Provided herein is | 06-04-2009 |
20090148918 | Glycerol Feedstock Utilization for Oil-Based Fuel Manufacturing - The invention provides methods of manufacturing biodiesel and other oil-based compounds using glycerol and combinations of glycerol and other feedstocks as an energy source in fermentation of oil-bearing microorganisms. Methods disclosed herein include processes for manufacturing high nutrition edible oils from non-food feedstock materials such as waste products from industrial waste transesterification processes. Also included are methods of increasing oil yields by temporally separating glycerol and other feedstocks during cultivation processes. Also provided herein are oil-bearing microbes containing exogenous oil production genes and methods of cultivating such microbes on glycerol and other feedstocks. | 06-11-2009 |
20090148919 | Delta 6-Desaturase Genes and Uses Thereof - The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 6 (i.e., “Δ6-desaturase”). In particular, Δ6-desaturase may be utilized, for example, in the conversion of linoleic acid to γ-linolenic acid and in the conversion of α-linolenic acid stearidonic acid. The polyunsaturated fatty acids produced by use of the enzyme may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics. | 06-11-2009 |
20090155864 | SYSTEMS, METHODS, AND DEVICES FOR EMPLOYING SOLAR ENERGY TO PRODUCE BIOFUELS - A photo-bioreactor can be arranged to receive incident solar radiation. The photo-bioreactor can contain photosynthetic organisms. The photosynthetic organisms can be genetically modified to produce an organic substance. The organic substance can be a biofuel or a precursor to a biofuel. The precursor can be isolated and converted into a biofuel. The biofuel can be extracted from the photo-bioreactor for use, for example, in energy generation or as a fuel. | 06-18-2009 |
20090170173 | NUCLEIC ACID MOLECULES ENCODING POLYPEPTIDES INVOLVED IN REGULATION OF SUGAR AND LIPID METABOLISM AND METHODS OF USE VIII - This invention relates generally to nucleic acid sequences encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to | 07-02-2009 |
20090176284 | PROCESS FOR PREPARING CONCENTRATED POLYUNSATURATED FATTY ACID OIL - The present invention provides a process for concentrated PUFA oil, characterized in that alcoholysis reaction using lipase is carried out in the presence of a small amount of water and at least one compound as an additive selected from magnesium oxide, magnesium hydroxide, calcium oxide and calcium hydroxide, and then separation is conducted to obtain a glyceride fraction. | 07-09-2009 |
20090176285 | Compositions and Methods for Enhancing Glycerol Utilization - A glycerol utilizing cell and a method for the production of glycerol-derived target compounds are provided. The glycerol utilizing cell may comprise a glycerol metabolizing system or a glycerol uptake protein and be used to produce a glycerol-derivable target compound. | 07-09-2009 |
20090197312 | Production of fat from alcohol - The present invention concerns a process for forming a lipid or a mixture of lipids from a starting material, which comprises at least one alcohol and a soap or a soap precursor, the process comprising adding a metal-ion forming agent to the starting material, whereby a mixture is formed, which contains an insoluble phase and a liquid phase, separating the insoluble phase from the liquid phase, contacting the lipid-producing microorganism with the liquid phase on a culturing substrate, whereby the microorganism cells begin producing lipid, and collecting the lipids. The present invention also concerns a process for forming a lipid or a mixture of lipids from an alcohol-containing liquid phase, which comprises at least one alcohol. | 08-06-2009 |
20090203092 | Liquid Biofuel Mixture and Method and Device for Producing The Same - The present invention relates to a biofuel mixture comprising a fraction of fatty acid alkyl esters and at least one fraction of bound glycerol in an amount of ≧1 wt %, based on the glycerol backbone, and a method and a device for producing said biofuel mixture. | 08-13-2009 |
20090203093 | Protein Targeting To Lipid Bodies - The present invention relates to a method of targeting a protein of interest to an intracellular hydrophobic inclusion body of a bacterial cell by means of a fusion protein comprising a hydrophobic targeting peptide operatively linked with said protein of interest; methods of microbial production of a lipophilic compound of interest by means of a recombinant bacterial host comprising intracellular inclusion bodies having at least one enzyme which is involved in the biosynthesis of said lipophilic compound targeted to said inclusion bodies; as well as corresponding fusion proteins, coding sequences, expression vectors and recombinant hosts. | 08-13-2009 |
20090203094 | Use Of Genes For Increasing The Oil Content In Plants - The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing the Ypr140w polypeptide from yeast or corresponding polypeptides from plants. The invention furthermore relates to expression constructs for expressing the yeast polypeptide Ypr140w or corresponding polypeptides from plants in plants, preferably in plant seeds, the transgenic plants expressing the polypeptide and to the use of the transgenic plants for the production of food, feed, seed, pharmaceuticals or fine chemicals, in particular for the production of oils. | 08-13-2009 |
20090209014 | HETEROTROPHIC ALGAL HIGH CELL DENSITY PRODUCTION METHOD AND SYSTEM - A multiphase culturing process for high density heterotrophic microalgal growth uses crude glycerol as the primary carbon source and produces ω-3 fatty acids. The process uses multiphase growth conditions that decouple the phases of increasing cell density and increasing cell size and fatty acid production. The entire process is integrated with biodiesel production. | 08-20-2009 |
20090209015 | COMPOSITIONS AND METHODS FOR PRODUCTION OF BIOFUELS - Provided is a method for producing C4 to C14 fatty acids. The method entails providing genetically modified cells that express a heterologous animal TE-II and either a heterologous animal wild type FAS or a ΔTE-I-FAS. The TE-II and the heterologous wild-type or ΔTE-I-FAS are expressed as distinct molecules. The cells are cultured for a period of time, and the fatty acids are extracted either as free fatty acids or as glycerides from the cells and/or from the media in which the cells are cultured. | 08-20-2009 |
20090246839 | Enzymatic modification of triglyceride fats - Process wherein the fatty acid residues on a glyceride moiety are randomised over the terminal and middle positions, wherein the process proceeds to a conversion degree on the terminal positions, Re, ranging from 0.3 to 0.95, and wherein a conversion degree on the middle position, Ra, ranges from 0.06 to 0.75, and wherein Ra is greater than 0.32Re-0.08, the process comprises the exposure of a triglyceride fat to a catalyst comprising a lipase wherein the lipase is a Thermomyces lanuginosa lipase which has an activity of at least 250 IUN corresponding to 22 g/(g*h) at the onset of the process. | 10-01-2009 |
20090246840 | PROCESS FOR PRODUCING ISOMER ENRICHED CONJUGATED LINOLEIC ACID COMPOSITIONS - A process for the preparation of a composition comprising the cis9, trans11 and trans10, cis12 isomers of conjugated linoleic acid (CLA) comprises: providing a mixture comprising cis9, trans11 and trans10, cis12 isomers of conjugated linoleic acid (CLA) in which one of the cis9, trans11 and trans10, cis12 isomers is present in a first weight ratio X of at least 1.3:1 with respect to the other isomer; and subjecting the mixture to crystallisation to form a composition comprising the cis9, trans11 and trans10, cis12 isomers in which one of the cis9, trans11 and trans10, cis12 isomers is present at a second weight ratio Y with respect to the other isomer, wherein Y is greater than X. | 10-01-2009 |
20090253188 | DELTA-4 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS - Described here are Δ4 desaturases that convert all-cis-7,10,13,16,19-docosapentaenoic acid [“DPA”; 22:5 ω-3] to docosahexaenoic acid [“DHA”; 22:6 ω-3], with secondary activity in converting docosatetraenoic acid [“DTA”; 22:4 ω-6] to all-cis-4,7,10,13,16-docosapentaenoic acid [“DPAn-6”; 22:5 ω-6]. Also, described here are isolated nuclei acid fragments and recombinant constructs comprising such fragments encoding Δ4 desaturases as well as methods of making long chain polyunsaturated fatty acids [“PUFAs”] using this Δ4 desaturase in oleaginous yeast. | 10-08-2009 |
20090286292 | PRODUCING EICOSAPENTAENOIC ACID (EPA) FROM BIODIESEL-DERIVED CRUDE GLYCEROL - , when cultured with crude glycerol as a carbon source, produces useful polyunsaturated fatty acids such as eicosapentaenoic acid (EPA). The crude glycerol is pretreated to remove soaps and methanol. An exemplary | 11-19-2009 |
20090291479 | MANIPULATION OF ACYL-COA BINDING PROTEIN EXPRESSION FOR ALTERED LIPID PRODUCTION IN MICROBIAL HOSTS - Acyl-CoA binding protein [“ACBP”] binds thiol esters of long fatty acids and coenzyme A in a one-to-one binding mode with high specificity and affinity. This protein is expected to play an important role in altering lipid production in oleaginous microbial organisms. Knock-out of the protein in the oleaginous yeast, | 11-26-2009 |
20090298141 | Continuous Chemical Proceses in Centrifugal Contact Separators - The invention relates to the use of a centrifugal contact-separator for carrying out a non-radioactive reaction in a liquid-liquid emulsion formed from two immiscible liquids. The invention also relates to a process for carrying out a reaction in a centrifugal contact-separator, and to a process for carrying out a catalytic reaction in a centrifugal contact-separator. An example of a process for carrying out a reaction, comprising the following steps: i) continuously introducing a liquid phase A and a liquid phase B into at least one first centrifugal contact separator, where in liquid phases A and B are immiscible and wherein phase A and/or phase B comprise at least one reactant ii) mixing phase A and phase B thereby allowing an emulsion to be formed iii) applying a centrifugal force that allows phase separation of the emulsion, such that phases A′ and B′ are obtained; iv) optionally, recovering a reaction product from at least one or the phases A′ and B′. | 12-03-2009 |
20090298142 | METHOD FOR PRODUCING A USEFUL SUBSTANCE BY USE OF AN IMMOBILIZED ENZYME - The present invention provides a method for producing a useful substance by supplying, to a fixed-bed reactor packed with an immobilized enzyme, a liquid mixture containing two liquid phases, in which the two liquid phases are allowed to flow in an identical, parallel direction, which method employs a fixed-bed reactor equipped with an insertion unit or tubes, so as to form a plurality of lumens in the fixed-bed reactor, each lumen having a cross section of a circular shape with a diameter of 100 mm or less or having a polygonal shape with a diagonal line of 100 mm or less, wherein the lumens are packed with an immobilized enzyme and the liquid mixture is supplied therethrough. In a reaction performed by passing a reaction mixture exhibiting two liquid phases through a fixed-bed reactor equipped with an immobilized enzyme, overall flow of the reaction liquid is made uniform, to thereby facilitate production of a useful substance in an efficient manner. | 12-03-2009 |
20090298143 | SECRETION OF FATTY ACIDS BY PHOTOSYNTHETIC MICROORGANISMS - Recombinant photosynthetic microorganisms that convert inorganic carbon to secreted fatty acids are described. Methods to recover the secreted fatty acids from the culture medium without the need for cell harvesting are also described. | 12-03-2009 |
20090305365 | FAD4, FAD5, FAD5-2, AND FAD6, NOVEL FATTY ACID DESATURASE FAMILY MEMBERS AND USES THEREOF - The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA. | 12-10-2009 |
20090317878 | NUCLEAR BASED EXPRESSION OF GENES FOR PRODUCTION OF BIOFUELS AND PROCESS CO-PRODUCTS IN ALGAE - Various embodiments provide, for example, vectors, expression cassettes, and cells useful for transgenic expression of nucleic acid sequences. In various embodiments, vectors can contain nuclear-based sequences of unicellular photosynthetic bioprocess organisms for the production of food- and feed-stuffs, oils, biofuels, starches, raw materials, pharmaceuticals or fine chemicals. | 12-24-2009 |
20090325247 | METHODS FOR PRODUCING CERAMIDE USING TRANSFORMED YEAST - The present invention provides methods for producing human ceramide in a yeast cell. | 12-31-2009 |
20100021976 | PRODUCTION OF POLYUNSATURATED FATTY ACIDS, NOVEL BIOSYNTHESIS GENES, AND NOVEL PLANT EXPRESSION CONSTRUCTS - The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 11 encoding polypeptides having desaturase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C | 01-28-2010 |
20100028961 | Biogical production of fuels - A process for the production of one or more fatty acids or derivatives thereof from a carbohydrate, which process comprises treating of Jerusalem Artichoke the carbohydrate with a micro-organism which converts the carbohydrate to a microbial oil comprising one or more fatty acids or derivatives thereof, in which the carbohydrate is inulin and/or is derived from Jerusalem Arti-choke. | 02-04-2010 |
20100028962 | Algal Medium Chain Length Fatty Acids and Hydrocarbons - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. | 02-04-2010 |
20100035312 | IMMOBILIZED INTERFACIAL ENZYMES OF IMPROVED AND STABILIZED ACTIVITY - Disclosed is a process for the preparation of an interfacial enzyme immobilized on an insoluble support, by providing a bi-phase system comprised of an aqueous buffer solution and at least one first organic solvent; mixing said interfacial enzyme with the bi-phase system provided; adding the support to the obtained mixture and mixing; and isolating from the mixture obtained in the last step the interfacial enzyme immobilized on said support. The produced enzyme is locked in its catalytically active confirmation, and thus exhibits improved activity and stability. Also disclosed are uses of the produced enzymes, particularly in the preparation of biodiesel. | 02-11-2010 |
20100041111 | Immobilized Enzymes and Methods of Using Thereof - The disclosed matter relates to immobilized enzymes and methods of use thereof. | 02-18-2010 |
20100041112 | Production of Biodiesel - The invention relates to the production of biodiesel feedstock and biodiesel. A process for producing a biodiesel utilising a thraustochytrid is provided. | 02-18-2010 |
20100041113 | DELTA 6 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ6 desaturases, which have the ability to convert linoleic acid [“LA”; 18:2 ω-6] to γ-linolenic acid [“GLA”; 18:3 ω-6] and/or α-linolenic acid [“ALA”; 18:3 ω-3] to stearidonic acid [“STA”; 18:4 ω-3]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ6 desaturases, along with methods of making long-chain polyunsaturated fatty acids [“PUFAs”] using these Δ6 desaturases in oleaginous yeast, are disclosed. | 02-18-2010 |
20100041114 | PROCESS FOR PRODUCING USEFUL SUBSTANCE WITH IMMOBILIZED ENZYME - The present invention relates to a process for producing a useful substance, which includes feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and performing a reaction while allowing the liquid mixture to flow in the same direction in a co-current manner, wherein the fixed bed-type reaction column has partition plates inserted in the longitudinal direction of the fixed bed-type reaction column so as to form a plurality of tube-shaped structures, wherein each tube-shaped structure has a lateral cross-section which is circular or polygonal in shape with at least a part being unclosed and has a representative length of | 02-18-2010 |
20100047883 | PROCESS FOR PRODUCING USEFUL SUBSTANCE USING IMMOBILIZED ENZYME - The present invention relates to a process for producing a useful substance, the process including feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and allowing the liquid mixture to flow in the same direction in a co-current manner to perform a reaction, wherein the packing thickness of the immobilized enzyme per stage of the fixed bed-type reaction column having an equivalent circular diameter of 35 mmφ or larger is 10 to 200 mm. | 02-25-2010 |
20100047884 | Method for Producing Biodiesel - The invention relates to the utilisation of fatty materials with substantial free fatty acid content in the production of biodiesel by the use of microbial enzymes that are effective in a solvent-free process for the production of esters of fatty acids and C | 02-25-2010 |
20100062500 | METHOD FOR THE REESTERIFICATION OR ESTERIFICATION OF SIDE CHAINS IN POLYMERS - The present invention relates to a process for esterifying or transesterifying side chains in polymers, comprising the following steps
| 03-11-2010 |
20100062501 | METHOD - The present application provides a method of reducing and/or removing diglyceride from an edible oil, comprising admixing an edible oil with an acyl acceptor substrate and a diglyceride:glycerol acyltransferase, wherein the diglyceride:glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. The diglyceride:glycerol acyltransferase can comprise the amino acid sequence motif GDSX. The present invention also relates to the use of a diglyceride:glycerol acyltransferase in the manufacture of an edible oil, for reducing and/or removing diglyceride from said edible oil and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff. | 03-11-2010 |
20100062502 | MANIPULATION OF SNF1 KINASE FOR ALTERED OIL CONTENT IN OLEAGINOUS ORGANISMS - Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast | 03-11-2010 |
20100068772 | Solubilization of algae and algal materials - Methods for solubilizing algae or algal material are provided to facilitate the recovery of oil or lipids, as well as hydrocarbons and carbohydrates, from algae or algal material. The methods involve contacting algae or algal material with an oxoacid ester or thioacid ester of phosphorus or a mixture of an oxoacid of phosphorus and/or an alcohol to form a mixture thereof under conditions effective to solubilize the algae or algal material. These methods optionally further comprise bioconversion of the solubilized algae or algal material to form a composition suitable for recovery of oils and non-oil chemicals. | 03-18-2010 |
20100075386 | MUTANT DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochrome b | 03-25-2010 |
20100075387 | DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in plants and oleaginous yeast are disclosed. | 03-25-2010 |
20100081177 | Decreasing RUBISCO content of algae and cyanobacteria cultivated in high carbon dioxide - Algae and cyanobacteria are genetically engineered to have lower RUBISCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) content in order to grow more efficiently at elevated carbon dioxide levels while recycling industrial CO | 04-01-2010 |
20100081178 | MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING TRIGLYCERIDES - This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more exogenous genes encoding a diacylglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides. | 04-01-2010 |
20100086979 | PRODUCTION OF OMEGA-3 FATTY ACIDS IN MICROFLORA OF THRAUSTOCHYTRIALES USING MODIFIED MEDIA - The present invention relates to improved methods for the production of omega-3 fatty acids by microorganisms belonging to the microflora of Thraustochytriales using a modified composition of the culture medium. In particular, the invention is directed to a process for the production of omega-3 fatty acids by culturing microflora, such as strains of | 04-08-2010 |
20100093046 | ENERGY PRODUCTION WITH HYPERTHERMOPHILIC ORGANISMS - The present invention relates to the field of degradation with hyperthermophilic organisms, and in particular to the use of hyperthermophilic degradation to produce heat and energy rich components including hydrogen and ethanol from a biomass. In some embodiments, a biomass is fermented in the presence of hyperthermophilic organisms to produce heat. The heat is used to heat a liquid which is used directly in a heat pump or radiant heat or to produce electricity or drive a steam turbine. In some embodiments, acetate is utilized as a substrate to produce energy by methanogenesis. | 04-15-2010 |
20100093047 | MICROBIAL PROCESSING OF CELLULOSIC FEEDSTOCKS FOR FUEL - A system and method are provided which utilize microbes to convert biomass feedstock into a fuel. In one aspect, a method of producing lipids includes receiving a feedstock including biomass, exposing the feedstock to microbes which are capable of converting the feedstock into lipids, and extracting produced lipids. | 04-15-2010 |
20100099154 | PUFA POLYKETIDE SYNTHASE SYSTEMS AND USES THEREOF - The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system. | 04-22-2010 |
20100112649 | COMPOSITIONS, METHODS AND USES FOR GROWTH OF MICROORGANISMS AND PRODUCTION OF THEIR PRODUCTS - Embodiments of the present invention report compositions, methods and uses for growth and/or production of byproducts, products and/or co-products from algal cultures. Certain embodiments of the present invention report using wastewaters to grow microorganisms for producing one or more products, byproducts or co-products from the microorganisms. Other embodiments report compositions and methods for rapid growth of any photosynthetic organism in culture. | 05-06-2010 |
20100112650 | POWDERY LIPASE PREPARATION, METHOD FOR PRODUCING THE SAME AND USE THEREOF - The present invention discloses a powdery lipase preparation which is a granulated material comprising a lipase derived from | 05-06-2010 |
20100120103 | Recombinant Production Docosahexaenoic Acid (DHA) in Yeast - The present invention relates to a specifically novel recombinant method of production of the omega-3 fatty acid, Docosahexaenoic acid by a potentially safe recombinant organism | 05-13-2010 |
20100129882 | HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils. | 05-27-2010 |
20100151538 | Cellulosic Cultivation of Oleaginous Microorganisms - Methods are disclosed for cultivation of oleaginous microorganisms on feedstocks including depolymerized cellulosic material such as corn stover, | 06-17-2010 |
20100151539 | Recombinant Microalgae Cells Producing Novel Oils - Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus. | 06-17-2010 |
20100151540 | METHOD FOR PROCESSING AN ALGAE MEDIUM CONTAINING ALGAE MICROORGANISMS TO PRODUCE ALGAL OIL AND BY-PRODUCTS - A device and method for processing an algae medium containing algae microorganisms to produce algal oil and by-products thereof. The method comprises pumping the algae medium through a flow-through hydrodynamic cavitation device, generating localized zones of reduced fluid pressure, creating cavitational features in the algae medium, collapsing those cavitation features, and disintegrating cell walls and intracellular organelles to produce algal oil and by-products. | 06-17-2010 |
20100159538 | METHOD FOR THE CHEMOENZYMATIC PRODUCTION OF FATTY ACID ESTERS - A chemoenzymatic process for the production of fatty acid esters comprising partially esterifying one or more fatty acid esters, under mild temperatures with an enzymatic catalyst, and optionally in the presence of one or more inert solvents, with a 1- to 5-fold molar excess of one or more water-containing aliphatic alcohols with boiling points between 60° C. and 120° C., then removing the water and unreacted alcohol(s) from the resulting pre-esterification product, followed by additional esterification up to 99.7%, chemically-catalyzed with, e.g., an acid or tin salt at slightly higher temperatures, optionally using one or more inert solvents, with a 1- to 4-fold molar excess of the same one or more aliphatic alcohols as employed in the preliminary esterification step. | 06-24-2010 |
20100159539 | METHODS AND SYSTEMS FOR PRODUCING BIOFUELS AND BIOENERGY PRODUCTS FROM XENOBIOTIC COMPOUNDS - The present invention provides methods and systems for producing biofuel and bioenergy products using, as starting raw material, xenobiotic materials or compounds. The xenobiotic materials or compounds may originate from industrial or chemical plants, municipal waste, pharmaceutical products, cosmetic and personal care products, or other sources, and may include aliphatic and aromatic hydrocarbons, chlorinated organic solvents and other halogenated hydrocarbons, as well as heteroaromatic compounds. In accordance with the invention, these materials act as a carbon source to support the metabolism of xenobiotic-degrading microorganisms, thereby producing biomass and/or biogas that may be converted to bioenergy products by microbial synthesis. For example, the biomass may be converted to products such as ethanol, methanol, butanol, and methane, among others. The biogas may be converted to hydrogen gas and biodiesel, among others. Thus, the present invention couples the microbial breakdown (decomposition) of xenobiotic materials with the microbial synthesis of biofuel, thereby supplying needed (inexpensive) energy products, while reducing environmental pollution and contamination, and reducing the costs associated with disposal of hazardous waste. | 06-24-2010 |
20100159540 | SYNTHESIS OF RESOLVINS AND INTERMEDIATES, COMPOUNDS PREPARED THEREBY, AND USES THEREOF - Methods are disclosed for the preparation of a new class of lipid mediators known as resolvins, with Resolvin D6 (4,17-dihydroxy-5E,7Z,10Z,13Z,15E,19Z-docosahexaenoic acid) being exemplary. Also disclosed are methods for the efficient synthesis of key intermediates in the preparation of such resolvins, such as isotopically labeled ω-3 fatty acid metabolites, and derivatives and analogs thereof. The invention likewise extends to the intermediates so prepared, and to the resolvins prepared with their use. | 06-24-2010 |
20100167359 | METHODS FOR PRODUCING POLYUNSATURATED FATTY ACID AND LIPID CONTAINING POLYUNSATURATED FATTY ACID - A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA, a microbial cell containing a PUFA, and use of the microbial cell are provided. A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA including culture of a microorganism capable of producing arachidonic acid (ARA) and/or dihomo-gamma-linolenic acid (DGLA) is provided, the method including at least one of the following steps: (a) adding an organic acid in an amount of 0.01 to 5 w/v % to a culture medium after the beginning of main culture; (b) increasing the pH of the culture medium to a range effective for culture after the beginning of the main culture; and (c) adding a metal sulfate in an amount of 0.01 to 0.5 w/w % to the main culture medium. | 07-01-2010 |
20100184168 | PROCESS FOR PRODUCING FATTY ACIDS - A process for producing fatty acid by hydrolyzing oil and fat, which comprises: a first step of partially hydrolyzing oil and fat by either of the following method (a) or (b), and a second step of hydrolysis by the other method: (a) enzymatic hydrolysis using an immobilized enzyme which is an enzyme immobilized on a support and (b) high pressure and temperature hydrolysis. A method of efficiently producing fatty acid with a reduced content of trans-unsaturated fatty acid in the constituent fatty acids and having a good appearance with reduced coloring by hydrolysis of oil and fat is provided. | 07-22-2010 |
20100184169 | Modified Photosynthetic Microorganisms With Reduced Glycogen and Their Use in Producing Carbon-Based Products - This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen as compared to a wild type | 07-22-2010 |
20100190220 | METHOD FOR PRODUCING EPA-ENRICHED OIL AND DHA-ENRICHED OIL - Alcoholysis of oils and fats containing EPA and DHA is performed by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of a reaction additive such as magnesium oxide; then the glyceride fraction is separated; alcoholysis of the glyceride fraction is performed by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of a reaction additive such as magnesium oxide; and EPA-enriched oil and DHA-enriched oil are simultaneously obtained. | 07-29-2010 |
20100196971 | SEQUESTRATION OF COMPOUNDS FROM MICROORGANISMS - The invention provides novel mesoporous particles and methods for the selective sequestration of organic compounds from microorganisms, such as from various genera and species of algae that produce important organic compounds. The organic compounds can be selectively sequestered, for example, in favor of biodiesel impurities such as sterols and chlorophyll, to provide substantially pure free fatty acids. The free fatty acids can then be esterified to provide pure biodiesel. | 08-05-2010 |
20100209982 | MODIFIED-IMMOBILIZED ENZYMES OF HIGH TOLERANCE TO HYDROPHILIC SUBSTRATES IN ORGANIC MEDIA - Disclosed are preparations of modified interfacial enzymes, particularly lipases and phospholipases, immobilized on a solid support, wherein the enzyme is surrounded by hydrophobic microenvironment, thereby protected from deactivation and/or aggregation in the presence of hydrophilic agents, substrates and/or reaction products. The enzyme may be protected by being covalently bonded with lipid groups which coat the enzyme, or by being immobilized or embedded in a hydrophobic solid support. Also disclosed are processes for the preparation of the hydrophobically protected enzymes. The enzymes may be efficiently used in the preparation of biodiesel. | 08-19-2010 |
20100216198 | METHOD FOR THE SYNTHESIS OF OMEGA-UNSATURATED FATTY ACIDS - The invention relates to a method for the synthesis of short-chain ω-unsaturated fatty acids of general formula CH | 08-26-2010 |
20100233767 | PROCESS FOR THE RECOVERY OF MAGNESIUM FROM A SOLUTION AND PRETREATMENT - A process for the recovery of magnesium from a solution containing soluble magnesium, the process comprising, precipitating magnesium hydroxide from the solution, forming an oxide blend including magnesium oxide derived from the precipitated magnesium hydroxide together with calcium oxide, reducing the oxide blend to form a magnesium metal vapour and condensing the vapour to recover magnesium metal. | 09-16-2010 |
20100240106 | MUTANT ENZYMES - This invention relates to mutant enzymes with enhanced properties and processes for oxidation of organic compound substrates using such enzymes. | 09-23-2010 |
20100255550 | Regulatory Factors Controlling Oil Biosynthesis In Microalgae And Their Use - The production of transgenic plant oils is described by transfecting plant host cells with heterologous transcription inducers. Such inducers are specific for endogenous biosynthetic plant oil genes, such that the inducers induce an overexpression of the plant oil genes. The overexpressed gene product (i.e., for example, diacylglycerol transferase) thereby results in a increased intracellular production of a plant oil (i.e., for example, triacylglycerol). In one embodiment, the transfected plant host cell can be a plant algae species (i.e., for example, | 10-07-2010 |
20100255551 | MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING TRIGLYCERIDES - This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more exogenous genes encoding a diacylglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides. | 10-07-2010 |
20100261235 | CONTINUOUS PRODUCTION OF BIODIESEL FUEL BY ENZYMATIC METHOD - A method for continuously producing a fatty acid ester of the present invention comprises (a) mixing and agitating an oil and fat starting material and a lower alcohol, and supplying a mixture to one of the catalyst reaction tubes filled with a lipase; (b) producing a fatty acid ester and glycerin in the catalyst reaction tube; (c) introducing an outflowing liquid from the catalyst reaction tube into a glycerin separation tank, thereby collecting the glycerin; (d) adding a lower alcohol to a separated liquid obtained by separating the glycerin from the outflowing liquid, mixing and agitating an obtained material, and supplying a mixture to a following catalyst reaction tube; (e) repeating the steps (b) to (d) until supply to a last catalyst reaction tube is performed; and (f) collecting a fatty acid ester from the separated liquid obtained from the last catalyst reaction tube. According to the method of the present invention, the concentration of a lower alcohol can be strictly controlled and by product glycerin can be automatically removed. | 10-14-2010 |
20100267097 | FIBROUS MATERIALS AND COMPOSITES - Fibrous materials, compositions that include fibrous materials, and uses of the fibrous materials and compositions are disclosed. For example, the fibrous materials can be operated on by a microorganism to produce ethanol or a by-product, such as a protein or lignin. | 10-21-2010 |
20100267098 | DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ49 elongases, which have the ability to convert linoleic acid (LA; 18:2 ω-6) to eicosadienoic acid (EDA; 20:2 ω-6) and/or α-linolenic acid (ALA; 18:3 ω-3) to eicosatrienoic acid (ETrA; 20:3 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in oleaginous yeast are disclosed. | 10-21-2010 |
20100267099 | DELTA-8 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to Δ8 desaturase genes, which have the ability to convert eicosadienoic acid (EDA; 20:2 ω-6) to dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) and/or eicosatrienoic acid (ETrA; 20:3 ω-3) to eicosatetraenoic acid (ETA; (20:3 ω-3). Isolated nucleic acid fragments and recombinant DNA constructs comprising such fragments encoding Δ8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ8 desaturases in oleaginous yeast are disclosed. | 10-21-2010 |
20100285545 | BIOSYNTHETIC ROUTES TO LONG-CHAIN ALPHA,OMEGA-HYDROXYACIDS, DIACIDS AND THEIR CONVERSION TO OLIGOMERS AND POLYMERS - A substantially pure | 11-11-2010 |
20100297713 | MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENESIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to mutant Δ8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 ω-6, EDA] to dihomo-γ-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 ω-3, ETrA] to eicosatetraenoic acid [20:3 ω-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant Δ8 desaturases in plants and oleaginous yeast are disclosed. | 11-25-2010 |
20100297714 | Multi-Step Method for Producing Algae Products - A multi-step method for producing an algae product comprising, a microorganism consumption step, another step, and an algae product collection step. The microorganism consumption step comprises, combining a liquid growth medium comprising microorganisms with a phagotrophic algae capable of producing a desired algae product, consuming said microorganisms by said phagotrophic algae, and growing said phagotrophic algae. Another step comprises either a microorganism growth step or a photosynthetic algal growth step. A microorganism growth step comprises providing a liquid growth medium comprising nutrients and microorganisms capable of said consuming said nutrients, consuming said nutrients by said microorganisms, and growing said microorganisms. A photosynthetic algal growth step comprises providing a substantially organic nutrient depleted liquid medium, providing a microorganism population comprising said phagotrophic algae, photosynthetic growth of said phagotrophic algae. An algae product collection step comprises collecting a desired algae product from said phagotrophic algae. | 11-25-2010 |
20100304452 | PROCESS OF PRODUCING OIL FROM ALGAE USING BIOLOGICAL RUPTURING - A process for production of biofuels from algae can include cultivating an oil-producing algae, extracting the algal oil, and converting the algal oil to form biodiesel. Extracting the algal oil from the oil-producing algae can include biologically rupturing cell wall and oil vesicles of the oil-producing algae using at least one enzyme such as a cellulose or glycoproteinase, a structured enzyme system such as a cellulosome, a virus, or combination of these materials. | 12-02-2010 |
20100317071 | Method for the Enzymatic Production of Fatty Alcohol and/or Fatty Acid - The invention relates to a method for the enzymatic production of C | 12-16-2010 |
20100317072 | OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION - Described are engineered strains of the oleaginous yeast | 12-16-2010 |
20100323413 | Production of Oil in Microorganisms - The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, edible oils, lubricants and plastics using sucrose feedstocks from sugar cane, sugar beets, and molasses for bioproduction processes. The disclosed processes utilize oleaginous microbes as a conversion technology to convert chemical energy produced by sugar cane, molasses and sugar beets into energy-containing oils and oil derivatives. Also provided herein are oleaginous microbes containing one or more exogenous sucrose utilization genes. | 12-23-2010 |
20100323414 | Production of Oil in Microorganisms - The invention provides methods of producing lipids by oleaginous microorganisms. The invention also provides genetically engineered oleaginous microorganisms and methods of cultivation for lipid production. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured such microorganisms. Exemplary oleaginous microorganisms include oleaginous fungi and such oleaginous fungi that have been genetically modified. | 12-23-2010 |
20100323415 | Emulsions in Enzymatic Reactions - A reaction medium for enzyme-catalyzed reactions is provided, comprising an oil-in-water emulsion including water, an emulsifier, an oil phase and at least one interfacially active enzyme, where the emulsion is produced by the phase inversion temperature process and has a droplet size of 50 to 400 nm. A method for the enzyme-catalyzed esterification, transesterification or hydrolysis of fatty acid alkyl esters and/or triglycerides is also provided where the oil-in-water emulsion is used as the reaction medium. | 12-23-2010 |
20100330628 | ACTIVATED LIPASES AND METHODS OF USE THEREFOR - Methods for enhancing a biological (e.g., catalytic) activity of a lipase, are provided. In some embodiments, the methods include the step of alkylating one or more cysteine residues present within the lipase. Also provided are modified polypeptides for which a biological activity is enhanced by the disclosed methods, methods for using the disclosed polypeptides, including for the transesterification of renewable oils to produce a biofuel, and cell free systems that include a lipase, to which one or more moieties, such as steroidal moieties, are conjugated. | 12-30-2010 |
20100330629 | ROBUST MULTI-ENZYME PREPARATION FOR THE SYNTHESIS OF FATTY ACID ALKYL ESTERS - Disclosed is an enzymatic process for the preparation of fatty acid alkyl esters, particularly fatty acids methyl esters (biodiesel) in a solvent-free microaqueous system, from a fatty acid source and an alcohol or alcohol donor, employing a robust lipase preparation that comprises at least two lipases separately or jointly immobilized on a suitable support, where one of the lipases has increased affinity to partial glycerides, another is sn-1,3 positional specific, and an optional third lipase has high selectivity towards sn-2 position of the glycerol backbone of the fatty acid source. | 12-30-2010 |
20110003350 | SYSTEM AND METHOD FOR HIGH-VOLTAGE PULSE ASSISTED AGGREGATION OF ALGAE - A method and device for aggregating algae in an aqueous solution is disclosed. The method can include providing an algae feed comprising a liquid and algae dispersed therein. The algae feed can be aggregated by applying a nanosecond pulsed electric field to the algae feed. The nanosecond pulsed electric field can include a plurality of electric pulses having a pulse duration ranging from 1 to 1,000 nanoseconds. The method can also include separating an aggregated algae stream from the algae feed and feeding the aggregated algae stream to a lipid extraction operation. | 01-06-2011 |
20110003351 | DELTA-5 DESATURASE AND USES THEREOF - The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 5 (i.e., “Δ5-desaturase”) and at carbon 6 (i.e., “Δ6-desaturase”) and to uses thereof. In particular, Δ5-desaturase may be utilized, for example, in the conversion of dihomo-γ-linolenic acid (DGLA) to arachidonic acid (AA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). Delta-6 desaturase may be used, for example, in the conversion of linoleic (LA) to γ-linolenic acid (GLA). AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics. | 01-06-2011 |
20110008854 | PROCESS FOR THE GENERATION OF ALGAL OIL AND ELECTRICITY FROM HUMAN AND ANIMAL WASTE, AND OTHER HYDROCARBON SOURCES - A method for the generation of electricity includes producing a paste from hydrocarbon waste, delivering the paste to a pyrolysis unit, rendering the paste into a gaseous product stream, allowing the stream to flow through a suitable filter device, extracting energy from the stream, producing electricity, converting water to hydrogen via a water gas shift reaction, allowing the reacted stream to flow to a reciprocating compressor, allowing any remaining stream to pass through compression, cooling, condensing, and storing carbon dioxide in a high pressure tank, allowing any remaining stream to be split, one part being combusted in a gas turbine producing electricity and the other part being recycled to the plasmolysis unit, injecting exhaust gas into a feed stream which flows to a degasification chamber, and allowing carbon dioxide and residual water from a storage tank to be expanded, passed through the heat exchanger and injected into the aquaculture feed. | 01-13-2011 |
20110014665 | Production of Oil in Microorganisms - The invention provides methods of cultivating oil-bearing microbes using cellulosic material. Also provided are microorganisms containing one or more exogenous genes that facilitate the use of cellulosic materials as a feedstock. Also provided are microorganisms and methods for manufacturing non-alcohol-based fuels and fuel feedstocks through a process of converting cellulosic materials into oils. | 01-20-2011 |
20110020883 | ACYL-ACP THIOESTERASE GENES AND USES THEREFOR - The present invention provides novel genes encoding Class II acyl-ACP thioesterases and variants thereof that are active on C8, C10, C12, C14, C16, and C18 acyl-ACP substrates. The thioesterases can be introduced into transgenic organisms, including microorganisms and photosynthetic organisms, for producing fatty acids and fatty acid products. | 01-27-2011 |
20110027841 | METHOD FOR PREPARATION OF OXYLIPINS - The invention provides methods of producing oxygenated derivatives of polyunsaturated fatty acids (PUFAs). | 02-03-2011 |
20110027842 | Method for Producing Monosaturated Glycerides - A process for producing a glyceride product which is enriched in monounsaturated fatty acids relative to the starting glyceride comprising the steps: (a) alcoholysis of triglycerides employing lipolytic enzymes selective for saturated fatty acids and/or lipolytic enzymes selective for the 1-position, the 3-position or both positions in a glyceride; and (b) separation of fraction A which is enriched in saturated fatty acid esters from fraction B which is enriched in monounsaturated glycerides. | 02-03-2011 |
20110045554 | HETEROLOGOUS EXPRESSION OF EXTREMOPHILE HEAT SHOCK PROTEINS AND CHAPERONES IN MICROORGANISMS TO INCREASE TOLERANCE TO TOXIC COMPOUNDS - The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as γ-prefoldin, where the heterologous protein is from an extremophile, such as an archaean. | 02-24-2011 |
20110045555 | ENZYMATIC MODIFICATION OF OIL - The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition. | 02-24-2011 |
20110045556 | ALGAL LIPID HARVEST USING MOLLUSKS FOR BIOFUELS PRODUCTION - Embodiments of the present disclosure provide for novel strategies to harvest algal lipids using mollusks which after feeding algae from the growth medium can convert algal lipids into their biomass or excrete lipids in their pseudofeces which makes algae harvesting energy efficient and cost effective. The bioconverter, filter-feeding mollusks and their pseudofeces can be harvested and converted to biocrude using an advanced thermochemical liquefaction technology. Methods, systems, and materials are disclosed for the harvest and isolation of algal lipids from the mollusks, molluscan feces and molluscan pseudofeces. | 02-24-2011 |
20110053229 | METHOD FOR PRODUCTION OF IMMOBILIZED ENZYME - Provided is a method of producing an immobilized lipolytic enzyme which has almost the same ability as that of an unused immobilized enzyme by effectively utilizing an immobilization carrier in a used immobilized enzyme which has been used in an esterification reaction. | 03-03-2011 |
20110059494 | D4 DESATURASES AND D5 ELONGASES - Disclosed are methods and compositions related to ONC-T18, D4-desaturases, D5 elongases, their isolation, characterization, production, identification, and use for fatty acid production, as well as organisms containing these compositions and organisms expressing them. | 03-10-2011 |
20110059495 | MANIPULATION OF AN ALTERNATIVE RESPIRATORY PATHWAY IN PHOTO-AUTOTROPHS - Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus | 03-10-2011 |
20110059496 | GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE AND PHOSPHOGLYCERATE MUTASE PROMOTERS FOR GENE EXPRESSION IN OLEAGINOUS YEAST - Promoter regions associated with the | 03-10-2011 |
20110081691 | GENE THAT INCREASES PRODUCTION OF PLANT FAT-AND-OIL AND METHOD FOR USING THE SAME - This invention is intended to search for a transcription regulator having a novel function of improving the amount of substances produced per individual plant and to enhance such properties in a plant. A chimeric protein resulting from the fusion of a transcription factor belonging a transcription factor family that includes a transcription factor comprising the amino acid sequence as shown in SEQ ID NO: 4 with the repressor domain is expressed in a plant. | 04-07-2011 |
20110091946 | Enzymatic Process for Obtaining a Fatty Ester - The aim of the present invention is to obtain fatty esters from fatty acids derived from vegetable oils and butters, through an enzymatic process, so that said esters can act as emollients, emulsifiers and co-emulsifiers with differentiated performance, having high spreadability and slidability compared to the prior-art products. More specifically, the present invention relates to an enzymatic process for obtaining a fatty ester, comprising: a) obtaining a fatty acid through the enzymatic hydrolysis (saponification) of vegetable oils and butters selected from cupuagu butter, sapucainha butter, ucu{acute over (υ)}ba butter, murumuru butter, palm olein, pataua oil, tucuma oil, inaja oil, passion fruit oil, pequi oil and nasturtium oil; b) reacting the fatty acid with a fatty alcohol selected from capric alcohol, caprylic alcohol, isoamylic alcohol, lauric alcohol, myristic alcohol, lorol (70:30 mixture of lauric alcohol and myristic alcohol), cetyl alcohol, stearyl alcohol, cetostearyl alcohol (70:30 mixture of cetyl alcohol and stearyl alcohol), sorbitol, sorbitan, glycerin, polyglycerin, other polyols and mixture thereof, in the presence of an enzyme, in at least one reactor, at a temperature of from 40 to 80 QC; c) stirring the reaction solution; d) removing moisture from the reaction medium; e) vacuum filtering said solution to obtain the fatty ester. The fatty esters obtained through the process of the present invention can be used in the preparation of cosmetic compositions. | 04-21-2011 |
20110091947 | High-Purity Purification Method for Omega-3 Highly Unsaturated Fatty Acids - The present invention relates a high-purity purification method for omega-3 highly unsaturated fatty acids. More specifically, it relates to a high-purity purification method for omega-3 highly unsaturated fatty acids which is both environmentally friendly and easy to implement and comprises the steps of: a) preparing a fatty acid ethyl ester (FAEE) by ethanolysis of a natural oil or fat, using ethanol, in the presence of an enzyme catalyst extracted from at least one microorganism selected from the group consisting of | 04-21-2011 |
20110097769 | MODIFIED MICROORGANISM USES THEREFOR - The invention provides a genetically modified microorganism that acquires the ability to consume a renewable feedstock (such as cellulose) and produce products. This organism can be used to ferment cellulose, one of the most abundant renewable resources available, and produce products. | 04-28-2011 |
20110097770 | Novel method to generate bioactive compounds in algae - The present invention discloses a novel microalgae-based method, called Microalgae Genomics Technology™ (MaGT), which can be used to generate novel compounds or increase the existing compounds in algae, and revealed some example of its potential applications. Specifically, this method utilizes microalgae genomics technology to manipulate the metabolic pathways in the genomes of microalgae and therefore induce the algae to produce novel secondary compounds or increase the concentration of existing compounds with a variety of commercial applications. | 04-28-2011 |
20110097771 | ISOLATED POLYNUCLEOTIDES AND POLYPEPTIDES AND METHODS OF USING SAME FOR INCREASING PLANT UTILITY - Provided are isolated polypeptides comprising the amino acid sequence at least 80% homologous to SEQ ID NO:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754, 764-771 or 772 with the proviso that the amino acid sequence is not as set forth by SEQ ID NO: 765 or 771, isolated polynucleotides comprising the nucleic acid sequence at least 80% identical to SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719, 724-741 and 755-763 with the proviso that the nucleic acid sequence is not as set forth by SEQ ID NO:756 or 762, and isolated polynucleotides selected from the group consisting of SEQ ID NOs:779-792 and methods of using same for increasing oil content, yield, growth rate, biomass, vigor, abiotic stress tolerance and/or nitrogen use efficiency of a plant. | 04-28-2011 |
20110111470 | Methods and Compositions for the Recombinant Biosynthesis of Fatty Acids and Esters - The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms. | 05-12-2011 |
20110124063 | Methods, Systems, and Compositions for Microbial Bio-Production of Biomolecules Using Syngas Components, or Sugars, as Feedstocks - This invention relates to microorganism cells that are modified to increase conversion of carbon dioxide and/or carbon monoxide to a product, such as a fatty acid methyl ester, and to related methods and systems. A pathway from the Calvin Benson Cycle to the product is provided, which in various embodiments involves use of heterologous proteins that exhibit desired enzymatic conversions. | 05-26-2011 |
20110129884 | METHOD FOR THE CULTIVATION OF MICROORGANISMS OF THE ORDER THRAUSTOCHYTRIALES - The present invention relates generally to the field of microorganism cell culture. It devises a cell culture medium for and a method of culturing microorganisms of the order Thraustochytriales, especially for the production of omega-3-fatty acids. Furthermore it devises the use of ammonium tartrate as nitrogen source for the cultivation of micro-organisms of the order Thraustochytriales. | 06-02-2011 |
20110136187 | Process - A process of water degumming an edible oil (preferably a crude edible oil) comprising the steps of: a) admixing approximately 0.1-5% w/w water with an edible oil (preferably a crude edible oil) and a lipid acyltransferase, b) agitating the admixture for between about 10 minutes and 180 minutes at about 45 to about 900 C, and c) separating the oil phase and the gum phase. Preferably said lipid acyltransferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridises under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ No. 49; and/or is a polypeptide having lipid acyltransferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence which as has 70% or more identity therewith. In one embodiment the lipid acyltransferase is preferably used in combination with a phospholipase C enzyme. A process for modifying the gum phase of a degummed oil using a lipid acyltransferase is also taught herein. | 06-09-2011 |
20110136188 | SYNTHESIS OF FATTY ALCOHOL ESTERS OF ALPHA-HYDROXY CARBOXYLIC ACIDS AND THEIR USE AS PERCUTANEOUS ABSORPTION ENHANCERS - The present invention provides a novel approach for the preparation of fatty alcohol esters of α-hydroxy carboxylic acids. In one form of the invention, the target fatty alcohol ester of α-hydroxy carboxylic acid is produced by converting a lower alkyl ester of α-hydroxy carboxylic acid into a fatty alcohol ester of α-hydroxy carboxylic acid via alcoholysis (i.e., transesterification). The transesterification process is an equilibrium reaction, catalyzed chemically (i.e., with acids or bases) or enzymatically, that is shifted in the desired direction to produce the desired product. One preferred way of shifting the reaction in the direction of the desired product is by reducing the concentration of one of the products (e.g., distillation of a lower-boiling alcohol as soon as it is formed). Another preferred way of shifting the reaction in the direction of the desired product is by increasing the concentration of one of the reactants (e.g., adding more of the starting ester). | 06-09-2011 |
20110136189 | Integrated Processes and Systems for Production of Biofuels Using Algae - A process for production of biofuels from biomass can include depolymerizing a biomass to form a feed. The feed can be formed by autotrophically growing algal biomass and extracting the feed therefrom. The algal feed can be converted to an algal oil by introducing the algal feed to an oil-producing algae under growth conditions sufficient to encourage formation of algal oil within the oil-producing algae. The algal oil can be extracted from the oil-producing algae and can further be converted to biodiesel. | 06-09-2011 |
20110151524 | PROCESS FOR PRODUCING BIODIESEL THROUGH LOWER MOLECULAR WEIGHT ALCOHOL-TARGETED CAVITATION - A method for producing fatty acid alkyl esters from biolipids through transesterification and/or esterification reactions uses a flow-through cavitation device for generating cavitation bubbles in a fluidic reaction medium. The fluidic medium is passed through sequential compartments in the cavitation device having varying diameters and inner surface features to create localized reductions in fluid pressure thus vaporizing volatile alcohols and creating an increased surface area and optimized conditions for the reaction to occur at the gas-liquid interface around the bubbles. | 06-23-2011 |
20110151525 | ENZYMATIC PRODUCTION OF UNSATURATED FATTY ACIDS ESTERS OF ASCORBIC ACID IN SOLVENT-FREE SYSTEM - The invention provides a cost-effective enzymatic process for the production of unsaturated FA esters of ascorbic acid, such as ascorbyl oleate or ascorbyl linoleate (E-304), in solvent-free system in which ascorbic acid esters of saturated FA, unsaturated free fatty acids and/or unsaturated fatty acid esters are used as feedstock. With this method pure products can even be achieved in a short response times. These products meet the needs of the industry by improving their solubility in oil and the increased antioxidant activity, especially in foods, cosmetics and medicine. | 06-23-2011 |
20110151526 | Branched-Chain Fatty Acids And Biological Production Thereof - A method for producing anteiso fatty acid is provided. The method comprises culturing a cell comprising at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes at least one of the following reactions: conversion of pyruvate to citramalate; conversion of citramalate to citraconate; conversion of citraconate to β-methyl-D-malate; conversion of β-methyl-D-malate to 2-oxobutanoate; or conversion of threonine to 2-oxobutanoate, under conditions allowing expression of the polynucleotide(s) and production of anteiso fatty acid. Optionally the cell further comprises at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes conversion of 2-oxobutanoate to 2-aceto-2-hydroxy-butyrate, conversion of 2-aceto-2-hydroxy-butyrate to 2,3-dihydroxy-3-methylvalerate, and/or conversion of 2,3-dihydroxy-3-methylvalerate to α-keto-3-methylvalerate. A cell that produces anteiso fatty acid and a method of using the cell to produce anteiso fatty acid also are provided. | 06-23-2011 |
20110151527 | PRODUCTION OF BIODIESEL, CELLULOSIC SUGARS, AND PEPTIDES FROM THE SIMULTANEOUS ESTERIFICATION AND ALCOHOLYSIS/HYDROLYSIS OF MATERIALS WITH OIL-CONTAINING SUBSTITUENTS INCLUDING PHOSPHOLIPIDS AND CELLULOSIC AND PEPTIDIC CONTENT - The present invention relates to a method for producing fatty acid alkyl esters as well as cellulosic simplified sugars, shortened protein polymers, amino acids, or combination thereof resulting from the simultaneous esterification and hydrolysis, alcoholysis, or both of algae and other oil containing materials containing phospholipids, free fatty acids (FFA), glycerides, or combination thereof as well as polysaccharides, cellulose, hemicellulose, lignocellulose, protein polymers, or combination thereof in the presence of an alcohol and an optional acid catalyst. | 06-23-2011 |
20110165637 | FATTY ACID-PRODUCING HOSTS - Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally deleted. The hosts preferably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recombinantly stable and growth-competent at 37° C. Methods of producing a fatty acid product comprising culturing such hosts at 37° C. are also described. | 07-07-2011 |
20110165638 | Integrated Bio-Digestion Facility - Methods and systems for processing organic waste material are provided. These methods and systems include integrating an anaerobic bio-digester and nutrient recovery module with a bio-production facility, which can locally provide feedstock for the bio-production facility, and can locally provide organic material for the anaerobic bio-digester. Methods and systems for integrating an anaerobic bio-digester with a gas cleaner are also provided, which can recover nutrients while cleaning the biogas produced by the anaerobic bio-digester. | 07-07-2011 |
20110165639 | REFINERY PROCESS TO PRODUCE BIOFUELS AND BIOENERGY PRODUCTS FROM HOME AND MUNICIPAL SOLID WASTE - The present invention provides methods of a process (SOLWASFUEL system) for producing biofuel and bioenergy products using, as starting raw material, home and municipal organic solid waste, including recalcitrant lignocellulosic materials of paper, cardboards, organic plastics, cellulose plants, and food waste. In accordance with the invention, home and municipal solid waste, can serve as a carbon source to support the metabolism of synthetic microorganisms to produce biofuels and bioenergy products. | 07-07-2011 |
20110183387 | New Mutant Yeast Strains Capable of Accumulating a Large Quantity of Lipids - The subject of the present invention is a novel mutant yeast strain, particularly a | 07-28-2011 |
20110189741 | PROCESS AND APPARATUS FOR EXTRACTING BIODIESEL FROM ALGAE - The present invention relates to a system for cultivation of algae, extraction of lipids and transesterification of the lipids to obtain biodiesel. The system comprises three sections, that is to say cultivation, extraction and storage and reaction. In the lipid extraction area there is an ultrasonic reactor wherein the external walls of the alga are ruptured together with those of the oil sac to permit the extraction of lipids in the transesterification area there is also an ultrasonic reactor which ruptures the molecules of the fluid which passes therethrough to accelerate the reaction and render it almost immediate. | 08-04-2011 |
20110201063 | Algae growth for biofuels - A method and apparatus of algal oil production including the steps of: control growing to provide intensive growth to supply starting means for algae farming; Farming algae from using primarily sunlight Processing algae from the farmed algae preferably using a wet extraction process: and wherein at least one of the steps includes use of a bag able to be interconnected to gas or liquid flows of at least one of water, CO | 08-18-2011 |
20110201064 | CONVERSION TO BIOENERGY FROM BIOMASS OF PROTISTAN GRAZERS FEEDING ON AQUATIC PLANT AND/OR ALGAE WHICH CAN SURVIVE AND UPTAKE GREENHOUSE GASES IN THE MIXTURE OF TOXIC GASES AND SUBSTANCE - Provided are a method for removing and/or absorbing greenhouse gases using aquatic plant and/or algae having a greenhouse gas absorbing performance and resistance to toxic substances, a method for producing bioenergy using predators with high fatty acids, and a method for producing bioenergy including cultivating aquatic plant and/or algae having a greenhouse gas absorbing performance and resistance to toxic substances, while supplying a waste gas mixture to the aquatic plant and/or algae, (S2) cultivating predators having a capability to prey on the aquatic plant and/or algae while supplying the aquatic plant and/or algae to the predators, (S3) harvesting the predators, and (S4) converting the harvested predators into bioenergy. | 08-18-2011 |
20110201065 | SYNTHESIS OF LONG-CHAIN POLYUNSATURATED FATTY ACIDS BY RECOMBINANT CELLS - The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. | 08-18-2011 |
20110201066 | PRODUCTION OF FATTY ACID ALKYL ESTERS BY USE OF TWO LIPOLYTIC ENZYMES - A method for producing fatty acid alkyl esters, wherein a solution comprising triglyceride and alcohol is contacted with a first lipolytic enzyme having a relatively higher activity on free fatty acids than on triglyceride and a second lipolytic enzyme having a relatively higher activity on triglyceride than on free fatty acids. | 08-18-2011 |
20110217743 | Method of Producing Lauric Acid-containing Oil or Fat - To provide a method for supplying lauric acid with algae. | 09-08-2011 |
20110223641 | MICROBIAL ENGINEERING FOR THE PRODUCTION OF FATTY ACIDS AND FATTY ACID DERIVATIVES - Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production. | 09-15-2011 |
20110229944 | FAD4, FAD5, FAD5-2, AND FAD6, Novel Fatty Acid Desaturase Family Members and Uses Thereof - The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA. | 09-22-2011 |
20110244532 | PRODUCTION OF BRANCHED CHAIN FATTY ACIDS AND DERIVATIVES THEREOF IN RECOMBINANT MICROBIAL CELLS - Recombinant microbial cells are provided which have been engineered to produce branched chain products such as branched fatty acid derivatives by the fatty acid biosynthetic pathway, and methods of making branched fatty acid derivatives using the recombinant microbial cells. | 10-06-2011 |
20110250658 | Recombinant Microalgae Cells Producing Novel Oils - Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus. | 10-13-2011 |
20110250659 | MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING LIPIDS - This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that contain one or more exogenous genes encoding a phospholipase and/or thioesterase, which are capable of producing an increased amount of lipids and/or fatty acids. This disclosure also describes genetically modified photosynthetic microorganisms that contain one or more exogenous genes encoding a diacyglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides, as well as photosynthetic microorganism comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. | 10-13-2011 |
20110256594 | REMOVAL OF NITROGEN FROM A CHLOROPHYLL OR PHEOPHYTIN CONTAINING BIOMASS - The present disclosure relates to refining a product from a biomass containing chlorophyll and/or pheophytins. In particular, a method of refining a product (such as a biofuel) from a photosynthetic organism is disclosed. The photosynthetic organism can be a naturally occurring organism or a genetically modified or altered organism. The method of refining comprises removing nitrogen to obtain the desired product. In some aspects, nitrogen is removed from a chlorophyll and/or pheophytin containing product by enzymatic degradation of chlorophyll and/or pheophytins and subsequent removal of the nitrogen | 10-20-2011 |
20110281312 | HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM FOR BIOCATALYTIC SYNTHESIS OF A STRUCTURED LIPID - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils. | 11-17-2011 |
20110287495 | HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS TO PRODUCE TRIGLYCERIDES - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils. | 11-24-2011 |
20110287496 | HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR BIOCATALYTIC SYNTHESIS OF STRUCTURED LIPIDS - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils. | 11-24-2011 |
20110287497 | ANAEROBIC ORGANISMS IN A PROCESS FOR CONVERTING BIOMASS - A method for integrating biological conversion of mixed acids to hydrocarbons, hydrocarbon-like molecules, biofuels, and combinations thereof. The method including introducing fermentation products to at least one microorganism chosen from the group consisting of heterotrophic microorganisms, photo-mixotrophic microorganisms, chemo-autotrophic microorganisms, and combinations thereof. | 11-24-2011 |
20110294173 | Process and Microorganisms for Production of Lipids - The present invention provides a process for producing lipids for biofuel or lubricant and | 12-01-2011 |
20110294174 | Tailored Oils Produced From Recombinant Heterotrophic Microorganisms - Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants. | 12-01-2011 |
20110294175 | INTEGRATED PROCESS FOR THE PRODUCTION OF BIO-OIL FROM MICRO-ORGANISMS - The cultivation of heterotrophic and phototrophic micro-organisms is integrated for the production of bio-oil for biofuels, wherein the overall algal suspension produced is first thickened, with recirculation of the excess water to cultivation containers, and then thermally treated at a high temperature. After cooling, a bio-oil phase is recovered together with a suspension rich in soluble carbohydrates and proteins which forms a nutritional/energy source for heterotrophic micro-organisms. | 12-01-2011 |
20110306100 | METHOD OF PRODUCING FATTY ACIDS FOR BIOFUEL, BIODIESEL, AND OTHER VALUABLE CHEMICALSPCT/ - The present invention relates to a method of producing fatty acids, by (i) inoculating a mixture of at least one of cellulose, hemicellulose, and lignin with at least one microorganism strain that produces one or more cellulases, hemicellulases and laccase, that hydrolyze at least one of cellulose, hemicellulose and lignin, under conditions to produce at least one of glucose, cellobiose, xylose, mannose, galactose, rhamnose, arabinose or other hemicellulose sugars; (ii) inhibiting growth of the at least one microorganism strain; (iii) inoculating the mixture of step (ii) with at least one algae strain that metabolizes the at least one of glucose, cellobiose, xylose, mannose, galactose, rhamnose, arabinose or other hemicellulose sugars, under conditions so that the at least one algae strain produces one or more fatty acids; and optionally (iv) recovering the one or more fatty acids from the at least one algae strain. | 12-15-2011 |
20110306101 | METHOD OF PRODUCING FATTY ACIDS FOR BIOFUEL, BIODIESEL, AND OTHER VALUABLE CHEMICALS - A method of producing fatty acids, by: (i) inoculating in a first bio-reactor a mixture of at least one of cellulose, hemicellulose, and lignin with a microorganism strain that produces one or more cellulases, hemicellulases, and/or laccases that hydrolyze at least one of cellulose, hemicellulose and lignin, and culturing the mixture to produce at least one fermentation product, for example one or more alcohols, in the mixture; (ii) optionally removing a portion of the at least one fermentation product from the mixture; (iii) transferring the remaining portion of the mixture into a second bio-reactor; and (iv) inoculating the portion of the mixture in the second bio-reactor with an algae strain that is capable of metabolizing the at least one fermentation product and any of the remaining soluble sugars, and culturing the mixture under conditions so that the at least one algae strain produces one or more fatty acids. | 12-15-2011 |
20110306102 | IMPROVED METHODS FOR FERMENTATIVE PRODUCTION OF DOCOSAHEXAENOIC ACID - The invention comprises a process of producing polyunsaturated fatty acids from Thraustochytriales at high availability of oxygen by solid state fermentation or submerged fermentation in a batch, semi-continuous or continuous mode. Batch fermentation of more than 200 g/L and up to 450 g/L of dextrose added right in the beginning of fermentation was achieved with productivity of DHA achieved for higher than 10 g/Liter. The process comprises maintaining at least 20% to 30% dissolved oxygen and sometimes up to around 50% or more. | 12-15-2011 |
20110312045 | FERMENTATION PROCESSES - The present invention provides a method for the production of at least one of a fermentation product and a derivate thereof comprising the steps of: a. providing a fermentable aqueous solution comprising at least one fermentable compound and at least one impurity; b. selectively extracting the at least one impurity from at least a portion of the fermentable aqueous solution by means of an extractant to form an impurity-depleted fermentable solution comprising a fermentable compound and to form an impurity-comprising extract; and c. fermenting the fermentable compound of the impurity-depleted fermentable solution to form a fermentation broth comprising a fermentation product wherein the extractant comprises at least one of the fermentation product and a derivate thereof. | 12-22-2011 |
20120015413 | Microorganisms with Extended Substrate Utilization Range - The present invention relates to a microorganism of the genus | 01-19-2012 |
20120021470 | Chimeric PUFA Polyketide Synthase Systems and Uses Thereof - Disclosed are chimeric polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from | 01-26-2012 |
20120028318 | FUNGAL CUTINASE FROM MAGNAPORTHE GRISEA - Described are compositions and methods relating to a fungal cutinase cloned from | 02-02-2012 |
20120028319 | CHLORELLA SPECIES CONTAINING EXOGENOUS GENES - The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims. | 02-02-2012 |
20120034662 | CO-CULTURING ALGAL STRAINS TO PRODUCE FATTY ACIDS OR HYDROCARBONS - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. | 02-09-2012 |
20120040419 | METHOD FOR PRODUCING OIL BY CYSTOFILOBASIDIUM SPP. - An oil generation system is provided. The oil generation system includes a saccharide; and a working microorganism reacting with the saccharide to produce an oil, wherein the working microorganism has a genus being a | 02-16-2012 |
20120040420 | Flavor Compound-Producing Yeast Strains - Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds. | 02-16-2012 |
20120045800 | Microalgae Biofuel Production System - A system for processing oil from algae is disclosed. Specifically, the system recycles byproducts of the process for use as nutrients during algae growth and oil production. The system includes a conduit for growing algae and an algae separator that removes the algae from the conduit. Also, the system includes a device for lysing the algae and an oil separator to remove the oil from the lysed matter. Further, the system includes a biofuel reactor that receives oil from the oil separator and synthesizes biofuel and glycerin. Moreover, the algae separator, oil separator and biofuel reactor all recycle byproducts back to the conduit to support further algae growth. | 02-23-2012 |
20120058525 | METHOD FOR PRODUCING OIL BY YEAST - A method for producing oil by using yeast is provided. The method comprises utilizing at least one yeast strain serving as a working microorganism to produce the oil, wherein the yeast strain is genus | 03-08-2012 |
20120058526 | Methods and Systems for Processing Sugar Mixtures and Resultant Compositions - A method including: (a) selectively reacting a first sugar in a mixture which includes at least one second sugar to form a product mixture comprising a product of said first sugar; (b) separating said product of said first sugar from said product mixture; and (c) separating at least one of said at least one second sugar from said product mixture. | 03-08-2012 |
20120058527 | CAL A-RELATED ACYLTRANSFERASES AND METHODS OF USE, THEREOF - Compositions and methods relating to lipase/acyltransferase enzymes identified in prokaryotes and eukaryotes are described. These enzymes can be used in such applications as lipid stain removal from fabrics and hard surfaces and chemical synthesis reactions. | 03-08-2012 |
20120070868 | PRODUCTION OF ODD CHAIN FATTY ACID DERIVATIVES IN RECOMBINANT MICROBIAL CELLS - Recombinant microbial cells are provided which have been engineered to produce fatty acid derivatives having linear chains containing an odd number of carbon atoms by the fatty acid biosynthetic pathway. Also provided are methods of making odd chain fatty acid derivatives using the recombinant microbial cells, and compositions comprising odd chain fatty acid derivatives produced by such methods. | 03-22-2012 |
20120070869 | Methods of using Nannochloropsis Algal strains to produce hydrocarbons and fatty acids - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. More specifically, the invention relates to a | 03-22-2012 |
20120070870 | Methods and Molecules for Yield Improvement Involving Metabolic Engineering - The invention features methods and compositions relating to cells that have been engineered to reduce or eliminate proteins having enzymatic activity that interfere with the expression of a metabolic product. | 03-22-2012 |
20120077234 | METHOD AND SYSTEM FOR MICROBIAL CONVERSION OF CELLULOSE TO FUEL - A method and system are provided for producing biofuel from cellulosic feedstock. In the method, the cellulosic feedstock is pretreated to separate cellulose, hemicellulose, and lignin. Thereafter, the cellulose and hemicellulose are converted into sugars through enzymatic hydrolysis. Then, the sugars are converted into lipids, e.g. triglycerides, through a microbial process. Specifically, heterotrophic microalgae is grown on the triglycerides and forms triglycerides. While triglycerides are formed from the cellulose and hemicellulose, the lignin is converted into ringed hydrocarbons, such as aromatic hydrocarbons and cycloalkanes, e.g., cycloparaffins. To form the biofuel, the triglycerides and ringed hydrocarbons are processed together. During this step, the triglycerides are converted into straight chain paraffins and esters. Preferably, the biofuel is a surrogate for jet quality JP-8 fuel. | 03-29-2012 |
20120077235 | CHAIN-SELECTIVE SYNTHESIS OF FUEL COMPONENTS AND CHEMICAL FEEDSTOCKS - A method comprising providing a starting composition comprising a polyunsaturated fatty acid, a polyunsaturated fatty ester, a carboxylate salt of a polyunsaturated fatty acid, a polyunsaturated triglyceride, or a mixture thereof; self-metathesizing the starting composition or cross-metathesizing the starting composition with at least one short-chain olefin in the presence of a metathesis catalyst to form self-/cross-metathesis products comprising: cyclohexadiene; at least one olefin; and one or more acid-, ester-, or salt-functionalized alkene; and reacting cyclohexadiene to produce at least one cycloalkane or cycloalkane derivatives. A method for producing cycloalkanes for jet fuel by providing a starting composition comprising at least one selected from the group consisting of algal and polyunsaturated vegetable oils, subjecting the starting composition to metathesis to produce metathesis product comprising at least one olefin, cyclohexadiene, and at least one acid-, ester-, or salt-functionalized alkene, and reacting the at least one olefin and cyclohexadiene to form cycloalkane(s). | 03-29-2012 |
20120088279 | ALGAL LIPID PRODUCTION - Methods for increasing the levels of lipids in oleaginous algae are described. Lipid levels in algae can be increased by stress, such as nutrient stress, after which the lipid can be harvested from the algae using a non-destructive extraction process. The stress may be provided in a periodic or “pulsed” fashion. Lipid levels in oleaginous algae can also be increased using simulated stress by treating the algae with a chemical inhibitor or by using recombinant technology to insert a sequence expressing a protein such as a nitrate reductase inhibitor that is expressed when a stressed state is desired. A method for maintaining the temperature and water levels of algae ponds using buoyant spheres is also described. | 04-12-2012 |
20120094338 | CONTINUOUS PRODUCTION AND EXCRETION OF WAXY PRODUCTS FROM PHOTOSYNTHETIC ORGANISMS - This invention disclosed herein relates generally to production and maintenance of genetically modified organisms that are capable of continuously making and excreting valuable waxy products. | 04-19-2012 |
20120094339 | MICRO-ALGA BELONGING TO GENUS NAVICULA, PROCESS FOR PRODUCTION OF OIL BY CULTURE OF THE MICRO-ALGA, AND OIL COLLECTED FROM THE MICRO-ALGA - Disclosed are: a microalga highly capable of producing aliphatic hydrocarbons of 16 to 26 carbon atoms; a process for producing oil, which comprises a step of culturing the microalga; oil collected from the microalga; a fuel produced from the microalga; and a method for fixing carbon dioxide, which comprises a step of culturing the microalga. | 04-19-2012 |
20120094340 | Production of Ester-Based Fuels Such As Biodiesel From Renewable Starting Materials - Production of ester-based fuels such as biodiesel or jet fuel from renewable starting materials such as lignocellulosic material or algae is disclosed. Pulping and saccharification of the renewable starting materials produces carboxylic acids such as fatty acids or rosin acids, which are esterified via a gas sparged, slurry form of heterogeneous reactive distillation to yield ester-based fuels. | 04-19-2012 |
20120100581 | PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided. | 04-26-2012 |
20120115193 | TRANSFORMATION OF GLYCEROL AND CELLULOSIC MATERIALS INTO HIGH ENERGY FUELS - The present invention includes a system and method for making a biofuel comprising: providing a nitrogen-limiting, minimal growth media comprising glycerol, sugars generated from cellulosic biomass or both, under conditions in which an oleaginous microbe converts the growth media into at least one of triacylglycerol, neutral lipids, fatty acids, long-chain fatty acids, and hydrocarbons that is secreted by the microbe. | 05-10-2012 |
20120122164 | METHOD AND SYSTEM FOR PROCESSING A BIOMASS FOR PRODUCING BIOFUELS AND OTHER PRODUCTS - The present invention provides methods of processing a biomass, e.g., for producing lipid and non-lipid materials, by inducing autolysis of a biomass. The biomass may be generated using any species of microorganism and any available biodegradable substrate, including waste materials. The lipid and non-lipid materials are present in two separate layers of the autolysate, and they can be used to generate valuable products such as biofuels and nutritional supplements. The present invention further provides a processing apparatus useful for practicing the methods of the present invention. | 05-17-2012 |
20120135478 | ALGAL MEDIUM CHAIN LENGTH FATTY ACIDS AND HYDROCARBONS - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. More specifically, the invention relates to a | 05-31-2012 |
20120135479 | FRACTIONATION OF OIL-BEARING MICROBIAL BIOMASS - The invention generally relates to the production of hydrocarbon compositions, such as a lipid, in microorganisms. In particular, the invention provides methods for extracting, recovering, isolating and obtaining a lipid from a microorganism and compositions comprising the lipid. The invention also discloses methods for producing hydrocarbon compositions for use as biodiesel, renewable diesel, jet fuel, and other materials. | 05-31-2012 |
20120149074 | Transformant Comprising Gene Coding for ws/dgat and Method of Producing Fatty Acid Ethyl Esters Using the Same - The present invention relates to a transformant comprising a gene encoding ws/dgat (wax ester synthase/acyl-coenzyme A: diacylglycerol acyltransferase) and a method of producing fatty acid ethyl esters using the same. Specifically, the transformant for producing fatty acid ethyl esters is constructed such that glycerol is used as a fermentation substrate, and comprises an atfA gene encoding ws/dgat (wax ester synthase/acyl-coenzyme A: diacylglycerol acyltransferase) from | 06-14-2012 |
20120149075 | SOAPS PRODUCED FROM OIL-BEARING MICROBIAL BIOMASS AND OILS - Soap and cosmetic products can be made from oil-bearing microbial biomass via the alkaline hydrolysis of glycerolipids and fatty acid esters to fatty acid salts. The saponified microbial oils/lipids can be combined with a variety of additives to produce compositions for use as soaps and other cosmetic products, which may also contain other constituents of the biomass, including unsaponified oils, glycerol and carotenoids, among others. | 06-14-2012 |
20120164700 | PROKARYOTIC ACYL-ACP THIOESTERASES FOR PRODUCING FATTY ACIDS IN GENETICALLY ENGINEERED MICROORGANISMS - The described invention provides genetically engineered photosynthetic microorganisms expressing prokaryotic acyl-ACP thioesterases and methods of using the genetically engineered photosynthetic microorganisms for producing free fatty acids and/or fatty acid derivatives. | 06-28-2012 |
20120164701 | RENEWABLE CHEMICALS AND FUELS FROM OLEAGINOUS YEAST - The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims. | 06-28-2012 |
20120171735 | PRODUCTION OF TRIACYLGLYCERIDES, FATTY ACIDS, AND THEIR DERIVATIVES - The invention relates to production of triacylglycerols in cells. | 07-05-2012 |
20120171736 | POWDERY LIPASE PREPARATION AND USE THEREOF - The present invention discloses a powdery lipase preparation in which the particles constituting the powdery lipase have 3,000 to 40,000 pores/mm | 07-05-2012 |
20120178134 | ENHANCEMENT OF BIOMASS PRODUCTION BY DISRUPTION OF LIGHT ENERGY DISSIPATION PATHWAYS - The invention provides a method of producing biomass or at least one biomolecule comprising culturing a photosynthetic microorganism that comprises a disrupted Non-Photochemical Quenching (NPQ) process, and isolating biomass or at least one biomolecule from the culture. | 07-12-2012 |
20120178135 | ENHANCED PRODUCTION OF LIPIDS CONTAINING POLYENOIC FATTY ACIDS BY VERY HIGH DENSITY CULTURES OF EUKARYOTIC MICROBES IN FERMENTORS - The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids. | 07-12-2012 |
20120184001 | SYSTEMS AND METHODS FOR SUSTAINABLE AQUACULTURE - Provided herein are systems and methods for sustainable aquaculture. The methods provided herein allows recovery and/or recycling of autochthonous nutrients in fish farming, and recovery of allochthonous nutrients present in eutrophic water. The systems provided herein comprises two closely spaced cages, an array of rotary panels that regulates the flow of matters between the two cages; and a means for producing a directional water current. | 07-19-2012 |
20120184002 | CULTURING A MICROORGANISM IN A MEDIUM WITH AN ELEVATED LEVEL OF A CARBOXYLATE COUNTERION SOURCE - The invention provides a method of culturing a microorganism that produces a free fatty acid in a culture medium, comprising culturing the microorganism in the culture medium comprising a sufficient amount (e.g., at least about 0.5 mM) of a carboxylate counterion source to enhance viability of the microorganism in the culture medium. Fatty acids produced using the methods of the invention can be used to synthesize a variety of products, including biofuels. | 07-19-2012 |
20120184003 | PRODUCTION OF FATTY ACIDS AND FATTY ACID DERIVATIVES BY RECOMBINANT MICROORGANISMS EXPRESSING POLYPEPTIDES HAVING LIPOLYTIC ACTIVITY - The invention provides transgenic photosynthetic microorganisms that include non-native genes encoding polypeptides having lipolytic activity for production of free fatty acids and fatty acid derivatives, and methods of producing free fatty acids and fatty acid derivatives using the transgenic microorganisms disclosed herein. The invention also provides transgenic microorganisms that include non-native genes encoding polypeptides having lipolytic activity, and novel genes encoding polypeptides demonstrating lipolytic activity. | 07-19-2012 |
20120184004 | METHODS AND COMPOSITIONS FOR THE PRODUCTION OF FATTY ACIDS IN PHOTOSYNTHETIC PROKARYOTIC MICROORGANISMS - Provided herein are methods and compositions for the production of renewable fatty acids from photosynthetic prokaryotic microorganisms, such as a blue green algae, any specie of the phylum Cyanophyta, a chloroplast of a green algae and/or a Cyanobacterium. Engineered or natural strains of these organisms or organelles can be used to produce both saturated and unsaturated fatty acids with variable chain length specificity from 8-18 carbons. The fatty acids can then be secreted into the culture medium, allowing for rapid, continuous and efficient separation of fatty acid product without harvesting of cell mass. | 07-19-2012 |
20120190088 | METHOD OF PRODUCING FATTY ACID ALKYL ESTER USING MICROORGANISMS HAVING ABILITY TO PRODUCE OIL - The present invention relates to a method of producing a fatty acid alkyl ester using microorganisms having the ability to produce oil, and more particularly to a method of producing a fatty acid alkyl ester, the method comprising culturing microorganisms having the ability to produce oil, thus accumulating a large amount of oil in the microorganisms, inducing the autolysis of the produced oil in the microorganisms to produce a free fatty acid, and converting the free fatty acid into an alkyl ester. According to the method of the present invention, oil accumulated in microorganisms, such as triacylglycerol that is typical oil produced by microorganisms, can be converted into a fatty acid alkyl ester with high efficiency using a metabolic engineering approach. Thus, the method of the present invention is useful for the industrial production of a fatty acid alkyl ester which has been recently found to be effective as biodiesel. | 07-26-2012 |
20120196336 | RADIAL COUNTERFLOW REACTOR WITH APPLIED RADIANT ENERGY - An improvement is described for the processing of biological material in a continuous stream by the application of radiant energy taken from the wavelengths from infrared to ultraviolet, and its absorption by a feedstock in a workspace of featuring controlled turbulence created by one or more counter-rotating disk impellers. The absorbed energy and the controlled turbulence patterns create a continuous process of productive change in a feed into the reactor, with separated light and heavy product output streams flowing both inward and outward from the axis in radial counterflow. The basic mechanism of processing can be applied to a wide range of feedstocks, from the promotion of the growth of algae to make biofuel or other forms of aquaculture, to a use in the controlled combustion of organic material to make biochar. | 08-02-2012 |
20120196337 | HETEROGENOUS ENZYMATIC CATALYST, PREPARATION METHOD, AND USE - The invention relates to a heterogenous enzymatic catalyst that takes the form of a cellular monolith consisting of a silica or organically modified silica matrix, said monolith including macropores, mesopores, and micropores, said pores being interconnected, and wherein the inner surface of the macropores is functionalized by a coupling agent selected from among silanes, said inner surface moreover having an unpurified enzyme attached thereon by means of a covalent or electrostatic bond. The invention also relates to the method for preparing said catalyst, said method comprising: a first step for preparing a solid silica impression that takes the form of a cellular monolith such as defined above; a second step for functionalizing the inner surface of the macropores via a coupling agent, selected from among silanes, by vacuum-soaking the cellular monolith by dissolving the coupling agent in an organic solvent; and a third step for vacuum-soaking the thus-functionalized monolith by means of an aqueous solution or aqueous dispersion of at least one unpurified enzyme. Finally, the invention relates to the use of such catalyst to carry out catalyzed chemical reactions. | 08-02-2012 |
20120208246 | PRODUCTION OF ALCOHOL ESTERS AND IN SITU PRODUCT REMOVAL DURING ALCOHOL FERMENTATION - An alcohol fermentation process and composition that includes production of alcohol esters by esterification of product alcohol in a fermentation medium with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the product alcohol recovered from the alcohol esters. The carboxylic acid can also serve as an extractant for removal of the alcohol esters from the fermentation medium. | 08-16-2012 |
20120208247 | Non-Dispersive Process for Insoluble Oil Recovery from Aqueous Slurries - The development and application of a novel non-polar oil recovery process utilizing a non-dispersive solvent extraction method to coalesce and recover oil from a bio-cellular aqueous slurry is described herein. The process could apply to recovery of algal oil from a lysed or non-lysed algae slurry, recovery of Omega fatty acids from a bio-cellular aqueous feed, recovery of Beta-carotene from a bio-cellular aqueous feed and for the removal from produced water in oil production and similar type applications. The technique of the present invention utilizes a microporous hollow fiber (MHF) membrane contactor. The non-polar oil recovery process described herein can be coupled to a collecting fluid (a non-polar solvent such as heptane, a biodiesel mixture or the previously extracted oil) that is circulated through the hollow fiber membrane. In cases where the biodiesel mixture or the previously extracted oil is used the solvent recovery step (e.g. distillation) can be eliminated. | 08-16-2012 |
20120214212 | Expression and application of recombinant FAEES in algae as a novel method to produce biodiesel in vivo - This discovery is related to the production of bioenergy fuels in vivo, specifically related to the production of biodiesel fuels using algae. We express FAEES (fatty acid ethyl ester synthase) in algae, and use the recombinant FAEES (fatty acid ethyl ester synthase) in the algae to synthesize biodiesel, fatty acid ethyl ester or fatty acid methyl ester, in vivo from fatty acids in the presence of ethanol/or methanol. | 08-23-2012 |
20120231513 | SYSTEMS AND METHODS FOR CULTURING ALGAE WITH BIVALVES - Provided herein are systems and methods for extracting lipids and/or producing biofuel from algae in marine and freshwater environments, wherein algae and bivalves are co-cultured in a system of enclosures comprising water that comprises recycled nutrients that are essential for algal growth. The system also include enclosures for culturing fishes which are used to harvest the algae. | 09-13-2012 |
20120237987 | BACTERIUM FOR PRODUCTION OF FATTY ACIDS - The present invention encompasses a bacterium that produces fatty acids. | 09-20-2012 |
20120244584 | ENGINEERING MICROORGANISMS - The present disclosure provides, inter alia, | 09-27-2012 |
20120244585 | METHODS OF AND SYSTEMS FOR PRODUCING BIOFUELS FROM ALGAL OIL - A method for producing biofuels is provided that includes dewatering intact algal cells to make an algal biomass, extracting neutral lipids from the algal biomass, and esterifying the neutral lipids with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method further includes hydrodeoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock. The method further includes supplying the hydrogenation and deoxygenation processes with hydrogen produced from reformed light hydrocarbons or an algae culture. | 09-27-2012 |
20120252079 | YARROWIA DIACYLGLYCEROL ACYLTRANSFERASE PROMOTER REGIONS FOR GENE EXPRESSION IN YEAST - Promoter regions associated with the | 10-04-2012 |
20120252080 | METABOLIC PATHWAY TARGETING BY TRANSCRIPTION FACTOR OVEREXPRESSION - The invention provides microorganisms such as cyanobacteria genetically engineered to express proteins that include transcription factor domains for upregulation of lipid biosynthetic pathways. In addition to expression a gene encoding a transcription factor domain protein, the recombinant microorganisms can express at least one exogenous gene that encodes a polypeptide for the production of a fatty acid, fatty acid derivative, or triglyceride. Also included are methods of producing a fatty acid, fatty acid derivative, or triglyceride using the engineered microorganisms described herein as well as nucleic acid molecules encoding novel transcription factor domain proteins. | 10-04-2012 |
20120258505 | HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils. | 10-11-2012 |
20120270283 | ENZYME INTERESTERIFICATION PROCESS - A fatty material enzyme interesterification process comprising the steps of: a) providing a fatty material that has optionally undergone at least one prior purification treatment; b) introducing soap into said fatty material to form a soap-containing fatty material mixture, c) homogenising said soap-containing fatty material mixture by mixing, d) contacting said soap-containing fatty material mixture with lipase to produce a soap-containing interesterified fatty material, e) removing soaps from said soap-containing interesterified fatty material to yield interesterified fatty material, wherein said soap is either formed in-situ by addition of aqueous alkali to said fatty material or said soap is introduced directly into said fatty material. | 10-25-2012 |
20120270284 | Method And System For Robotic Algae Harvest - A Robotic Algae Harvester (RAH) of the present invention works by providing a CO | 10-25-2012 |
20120276602 | Method For Treatment of Oil - The present invention relates to a method for treatment of oil with a lipolytic enzyme which comprises contacting of the oil with particles of base-containing porous amorphous silica. | 11-01-2012 |
20120282662 | METHODS OF AND SYSTEMS FOR PRODUCING DIESEL BLEND STOCKS - A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids from the algal biomass, and esterifying the neutral lipids with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method further includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock. | 11-08-2012 |
20120288906 | Process For Obtaining Fatty Acid Lower Alkyl Esters From Unrefined Fats And Oils - Described is a process for obtaining fatty acid C | 11-15-2012 |
20120301932 | EXPRESSION OF CALEOSIN IN RECOMBINANT OLEAGINOUS MICROORGANISMS TO INCREASE OIL CONTENT THEREIN - Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described. | 11-29-2012 |
20120309063 | DETERGENT COMPOSITIONS CONTAINING GEOBACILLUS STEAROTHERMOPHILUS LIPASE AND METHODS OF USE THEREOF - The present compositions and methods relate to a lipase cloned from | 12-06-2012 |
20120309064 | MUTANT DELTA-5 DESATURASES MUTATED IN THE HEME-BINDING MOTIF (HPGG) AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochome b | 12-06-2012 |
20120322116 | METHOD FOR TRANSFORMING STRAMENOPILES - Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class | 12-20-2012 |
20120329109 | Genetically Engineered Microorganisms That Metabolize Xylose - The invention provides methods of cultivating oil-bearing microbes using xylose as a fixed carbon source. Also provided are microorganisms that have been genetically engineered to metabolize xylose as a fixed carbon source allowing them to convert xylose into oils. Particular advantages of the processes provided herein include production of oils rather than alcohols through the microbial fermentation processes utilizing xylose. | 12-27-2012 |
20130005003 | Secretion of Fatty Acids by Photosynthetic Microorganisms - Recombinant photosynthetic microorganisms that convert inorganic carbon to secreted fatty acids are described. Methods to recover the secreted fatty acids from the culture medium without the need for cell harvesting are also described. | 01-03-2013 |
20130005004 | PRODUCING EICOSAPENTAENOIC ACID (EPA) FROM BIODIESEL-DERIVED CRUDE GLYCEROL - , when cultured with crude glycerol as a carbon source, produces useful polyunsaturated fatty acids such as eicosapentaenoic acid (EPA). The crude glycerol is pretreated to remove soaps and methanol. An exemplary | 01-03-2013 |
20130005005 | Modified Lipids Produced from Oil-Bearing Microbial Biomass and Oils - Chemically modified lipids and methods for their preparation are provided. | 01-03-2013 |
20130011889 | GENERATION OF TRIACYLGLYCEROLS - A method is disclosed for the generation of triacylglycerols from gums that have been separated from an oil product. The gums are treated with an enzyme having PLC activity, which results in the formation of diacylglycerols and phosphates, and treated with an enzyme having PLA activity, which results in the formation of lyso-phospholipids and free fatty acids. The diacylglycerols and the free fatty acids from these two separate reactions then combine in the presence of the enzymes to generate new triacylglycerol molecules. | 01-10-2013 |
20130029387 | MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS - A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from | 01-31-2013 |
20130034886 | PSEUDOMONAS AERUGINOSA STRAIN DEVELOPED FOR IMPROVING FATTY ACID CONTENT, AND METHOD OF MANUFACTURING THE SAME - Disclosed are novel | 02-07-2013 |
20130034887 | Tailored Oils Produced from Recombinant Oleaginous Microorganisms - Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants. | 02-07-2013 |
20130040349 | Method for capture carbon & storage (CCS process) from coal fuel gas and the storage as biofuels: oil, gasoline, biodiesel, jet fuel, ethanol, and methane - A Capture Carbon Storage (CCS) Process for the economical capture of carbon dioxide from coal fuel gas, and the storage of the carbon dioxide as lipid oil or the use of the environmentally begin oil for transportation. The lipid oil may be refined into gasoline, biodiesel fuel, jet fuel, ethanol, and methane which provide a renewable energy resource for the United State's future energy needs. It is a new fuel form which has been both chemically and physically altered to reduce the emissions of carbon dioxide. It is a coal cleaning/upgrading process which produces a refined fuel, Hydrogen, that may be used to produce electricity and an environmentally begin biofuel for transportation. The renewable fuel will produce no carbon foot print at the internal combustion engine's exhaust pipe. The CCS Process will produce a 100% reduction in carbon dioxide (CO | 02-14-2013 |
20130045515 | HETEROGENEOUS E. COLI FOR IMPROVING FATTY ACID CONTENT USING FATTY ACID BIOSYNTHESIS AND PREPARATION METHOD THEREOF - Disclosed are novel | 02-21-2013 |
20130052701 | ENZYMATIC TRANSESTERIFICATION/ESTERIFICATION PROCESSES EMPLOYING LIPASES IMMOBILIZED ON HYDROPHOBIC RESINS IN THE PRESENCE OF WATER SOLUTIONS - Disclosed are an enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food and detergent industries and a system therefor. The process utilizes enzymes immobilized on a hydrophobic resin mixed with a fatty acid source and an alcohol or alcohol donor in the presence of an alkaline or mild alkaline aqueous buffer, or in the presence of water or aqueous solution. The production process for fatty acid alkyl esters is carried out by transesterification or esterification simultaneously or sequentially. The biocatalyst activity is maintained with no significant activity losses in multiple uses and also avoids the accumulation of glycerol and water by-products or other hydrophilic compounds on the biocatalyst. | 02-28-2013 |
20130052702 | Methods for Enhancing By-Products From Fermentation Processes - The present invention relates to methods for enhancing yield and/or quality of by-products from fermentation processes used for producing fermentation products. The invention includes a process of fermenting starch-containing material into a fermentation product comprising a fermentation step wherein one or more enzymes are added before or during the fermentation step. More specifically, the present invention provides methods for increasing oil recovery yield from processes for producing fermentation products and further enhancing the quality of DDGS by removing fat content from DDGS that are produced as by-products in processes for producing fermentation products. | 02-28-2013 |
20130059350 | CONSTRUCTS AND METHODS FOR INCREASING YIELD OF FATTY ALCOHOLS IN CYANOBACTERIA - A construct containing a gene operably linked to a promoter having activity in cyanobacteria, and vector and cyanobacterium comprising such construct for increasing the yield of fatty alcohols in cyanobacterium is provided. | 03-07-2013 |
20130059351 | Thioesterase and a Method of Producing Fatty Acids or Lipids Using the Thioesterase - A thioesterase comprising an amino acid sequence set forth in SEQ ID NO: 1, a thioesterase gene encoding the thioesterase, a transformant comprising the gene, and a method of producing fatty acids or lipids using the transformant. | 03-07-2013 |
20130065282 | Fermentation Process - Methods and systems for the production of one or more lipid products from a gaseous substrate using a two stage fermentation process are provided. The method comprises providing a gaseous substrate comprising CO | 03-14-2013 |
20130065283 | PROCESS FOR OBTAINING FATTY ACID ALKYL ESTERS FROM LIPIDS IN A MEMBRANE CONTACTOR - The present invention relates to a new process for obtaining fatty acid alkyl esters from fatty acid containing lipids, i.e. mono-, di- and triglycerides and phospholipids, in a membrane contactor. | 03-14-2013 |
20130071889 | PRODUCTION METHOD FOR BIOFUEL - The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S | 03-21-2013 |
20130078684 | ACYL-ACP WAX ESTER SYNTHASES - The invention relates to acyl-CoA-independent methods of producing a wax ester in recombinant host cells engineered to express an acyl-ACP wax ester synthase, and an alcohol-forming acyl-ACP reductase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing an acyl-ACP wax ester synthase and/or an alcohol-forming acyl-ACP reductase, recombinant host cells expressing an acyl-ACP wax ester synthase and optionally an alcohol-forming acyl-ACP reductase, and systems for producing a wax ester via an acyl-CoA-independent pathway, are also provided. | 03-28-2013 |
20130078685 | OZONOLYSIS OF UNSATURATED FATTY ACIDS AND DERIVATIVES THEREOF - The invention relates to a method for the ozonolysis of unsaturated fatty acids and derivatives thereof, comprising a) ozonolysis of a fatty acid or of a derivative thereof in an organic phase comprising an organic solvent, and b) contacting the organic phase with an aqueous phase comprising catalase and preferably a buffer, where the fatty acid or the derivative thereof has a linear chain having at least eight carbon atoms, and the use of catalase for removing reactive oxygen species from a reaction mixture comprising ozonolysis products of an unsaturated fatty acid. | 03-28-2013 |
20130078686 | PRODUCTION AND SECRETION OF FATTY ACIDS AND FATTY ACID DERIVATIVES - The invention relates to acyl-CoA-independent methods of producing fatty alcohols in recombinant host cells engineered to express an alcohol-forming acyl-ACP reductase. The recombinant host cells may be photosynthetic microorganisms, such as cyanobacteria. Isolated nucleic acid molecules, vectors, and recombinant host cells expressing an alcohol-forming acyl-ACP reductase, and systems for producing fatty alcohols via an acyl-CoA-independent pathway, are also provided. Also provided are microorganisms engineered for the secretion of fatty acids and fatty acid derivatives, including fatty alcohols, and methods of producing fatty acid derivatives using such engineering microorganisms. | 03-28-2013 |
20130084611 | Novel Plant Acyltransferases Specific for Long-Chained, Multiply Unsaturated Fatty Acids - The invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids coding for polypeptides with acyltransferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids. The invention furthermore relates to the nucleic acid sequences, and constructs, vectors and organisms comprising the nucleic acid sequences. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use. | 04-04-2013 |
20130089899 | INDUSTRIAL FATTY ACID ENGINEERING GENERAL SYSTEM FOR MODIFYING FATTY ACIDS - Compositions and methods for a hybrid biological and chemical process utilizing chemotrophic microorganisms that converts syngas and/or gaseous CO2 and/or a mixture of CO2 gas and H2 gas into one or more desaturated hydrocarbons, unsaturated fatty acids, hydroxy acids, or diacids. | 04-11-2013 |
20130089900 | Extraction Solvents Derived from Oil for Alcohol Removal in Extractive Fermentation - In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol. | 04-11-2013 |
20130089901 | NOVEL THRAUSTOCHYTRID-BASED MICROALGAE, AND METHOD FOR PREPARING BIO-OIL BY USING SAME - The present invention relates to novel thraustochytrid-like microalgae having the ability to produce bio-oil, and a method of producing bio-oil using the same. The microalgae of the invention accumulate bio-oil at a high ratio in the cells when being cultured in glucose-containing medium, and thus can produce bio-oil in a high yield. Also, the microalgae can produce bio-oil using bean powder as a nitrogen source, and a product obtained by culturing edible bean powder as medium can be used as a raw material for producing food and feed. Also, the microalgae can produce bio-oil using non-food cellulosic biomass as a carbon source. Moreover, the use of non-food cellulosic biomass for production of bio-oil can overcome the factors limiting the development of bio-oil, including the unstable supply of food resources and an increase in the cost of raw materials, and can improve the commercial competitiveness of microbial fermentation oil. | 04-11-2013 |
20130095536 | CARBON DIOXIDE CAPTURE BOX - The present subject matter relates to a system comprising a first unit that generates a polluted air comprising carbon dioxide, and a second unit mounted on the first unit. The second unit comprises a solution comprising at least one alga suspended in a solvent to purify the polluted air. The solution comprising the at least one alga is agitated upon operation of the first unit. The system further comprises a channel to guide the polluted air from the first unit to the second unit. | 04-18-2013 |
20130095537 | PROCESS FOR PRODUCTION OF USEFUL SUBSTANCES - A process for producing a useful substance using microorganisms, and an object thereof to provide a method that shortens the culturing period of the microorganisms and improves the productivity of the useful substance. The speed at which microorganisms consume carbohydrates and/or hydrocarbon oxidation products can be increased and the productivity of a target useful substance can be improved by using a main culture initial medium in which the total concentration of carbohydrate and hydrocarbon oxidation product is less than 0.4% by weight in terms of the carbon equivalent. | 04-18-2013 |
20130102039 | Methods of Producing Triacylglyceride Compositions Comprising Tailored Oils - Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants. | 04-25-2013 |
20130102040 | USE OF ENDOGENOUS PROMOTERS IN GENETIC ENGINEERING OF NANNOCHLOROPSIS GADITANA - The present disclosure is directed to novel polynucleotide sequences for use in | 04-25-2013 |
20130102041 | ENZYMATIC PROCESS FOR SYNTHESIZING ESTOLIDES - This invention is a process for enzymatic synthesis of estolides through the reaction between stearic acid and methyl ricinoleate, using an immobilized lipase as the catalyst, in a solvent-free medium. This process prevents degradation of the product, reduces the formation of secondary products that generate color and odor in the lubricant, and allows recovery of the lipase and its subsequent reuse. | 04-25-2013 |
20130102042 | GENETICALLY MODIFIED FUNGI AND THEIR USE IN LIPID PRODUCTION - The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phosholipid: diacylglycerol acyltransferase (PDAT)activity it and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant | 04-25-2013 |
20130115664 | PROCESS AND SYSTEM FOR PRODUCING ALGAL OIL - A method for producing an algal oil is provided. The method includes continuously providing a growth medium and an algal strain to a bioreactor at a predetermined fluid flow rate; illuminating the growth medium and algal strain contained within the bioreactor by a first artificial light source for a time sufficient to effect lipid production by the algal strain; continuously withdrawing a portion of the growth medium and algal strain contained within the bioreactor at the predetermined fluid flow rate; and treating the withdrawn portion of the growth medium and algal strain to produce and isolate a lipid produced by the algal strain. | 05-09-2013 |
20130115665 | METHODS AND COMPOSITIONS FOR PRODUCING FATTY ALCOHOLS - Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described. | 05-09-2013 |
20130115666 | METHOD FOR PRODUCTION OF EUGLENA CONTAINING WAX ESTER AT HIGH COTENT, AND METHOD FOR PRODUCTION OF WAX ESTER - Provided is a method for producing a | 05-09-2013 |
20130122557 | YEAST STRAINS AND THEIR USES IN THE PRODUCTION OF LIPIDS - The invention is directed to isolated microorganisms, as well as biomasses, cultures, microbial oils, and compositions thereof. The invention also provides methods of producing the microbial oils and methods of using the isolated microorganisms, biomasses, and microbial oils. | 05-16-2013 |
20130122558 | MUTANT DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochrome b | 05-16-2013 |
20130130334 | BIOFUEL AND ELECTRICITY PRODUCING FUEL CELLS AND SYSTEMS AND METHODS RELATED TO SAME - A fuel cell comprising an anode electrode, a cathode electrode and a reference electrode electronically connected to each other; a first biocatalyst comprising a consolidated bioprocessing organism (e.g., a cellulomonad or | 05-23-2013 |
20130130335 | Purification Methods and Systems Related to Renewable Materials and Biofuels Production - Methods of producing renewable materials may include consuming a fermentation feedstock with a fermentation organism to produce a renewable material in fermentation broth; water may then be separated from the feedstock or broth using one or more phase separations, or the renewable material may be concentrated from the feedstock or broth using one or more phase separations. Methods of producing biofuel components may include consuming a lignocellulosic or sugar fermentation feedstock with a fermentation organism to produce either ethanol or butanol in fermentation broth; cooling the feedstock or broth to solidify at least some water therein; and separating the solidified water from the feedstock or broth using a solid-liquid phase separation. | 05-23-2013 |
20130130336 | CHAIN-SELECTIVE SYNTHESIS OF FUEL COMPONENTS AND CHEMICAL FEEDSTOCKS - A method comprising providing a starting composition comprising a polyunsaturated fatty acid, a polyunsaturated fatty ester, a carboxylate salt of a polyunsaturated fatty acid, a polyunsaturated triglyceride, or a mixture thereof; self-metathesizing the starting composition or cross-metathesizing the starting composition with at least one short-chain olefin in the presence of a metathesis catalyst to form self-/cross-metathesis products comprising: cyclohexadiene; at least one olefin; and one or more acid-, ester-, or salt-functionalized alkene; and reacting cyclohexadiene to produce at least one cycloalkane or cycloalkane derivatives. A method for producing cycloalkanes for jet fuel by providing a starting composition comprising at least one selected from the group consisting of algal and polyunsaturated vegetable oils, subjecting the starting composition to metathesis to produce metathesis product comprising at least one olefin, cyclohexadiene, and at least one acid-, ester-, or salt-functionalized alkene, and reacting the at least one olefin and cyclohexadiene to form cycloalkane(s). | 05-23-2013 |
20130137149 | PRODUCTION OF BIODIESEL BY YEAST FROM LIGNOCELLULOSE AND GLYCEROL - The invention is directed to a genetically modified microorganism for the extracellular production of free fatty acids and esters thereof, wherein said microorganism is characterised by a modified lipid biosynthesis metabolic pathway: for example reduced fatty acyl-coA synthetase activity that enables the microorganism to over-produced and secrete of esters of fatty acids (Biodiesel) into the surrounding medium, using one or more of: glucose, starch, lignocellulose and a glycerol-based substrate, as a carbon source. The invention further provides a method for the extracellular production of free fatty acids and esters thereof, comprising the use of said genetically modified organism, and a growth medium adapted for said method. | 05-30-2013 |
20130143281 | Chimeric PUFA Polyketide Synthase Systems and Uses Thereof - Disclosed are chimeric polyunsaturated fatty acid (PUFA) olyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from | 06-06-2013 |
20130143282 | ENGINEERED MICROBES AND METHODS FOR MICROBIAL OIL PRODUCTION - Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties. | 06-06-2013 |
20130143283 | Integrated Bio-Digestion Facility - Methods and systems for processing organic waste material are provided. These methods and systems include integrating an anaerobic bio-digester and nutrient recovery module with a bio-production facility, which can locally provide feedstock for the bio-production facility, and can locally provide organic material for the anaerobic bio-digester. Methods and systems for integrating an anaerobic bio-digester with a gas cleaner are also provided, which can recover nutrients while cleaning the biogas produced by the anaerobic bio-digester. | 06-06-2013 |
20130143284 | MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING TRIGLYCERIDES - This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more exogenous genes encoding a diacyglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides. | 06-06-2013 |
20130149753 | Application of Bacteriophages for the Control of Unwanted Bacteria in Biofuel Production Mediated by Non-Bacterial Reactive Agents - A method of reducing process interruptions in biofuel production systems by reducing the amount of unwanted bacteria in the biofuel production system in which the reduction is effected by the use of an effective amount of one or more types of bacteriophages virulent for at least some strains of the unwanted bacteria. | 06-13-2013 |
20130149754 | Optimization of Lipid Synthesis and Accretion - The present invention relates to a novel mutant strain of yeast, particularly a strain of | 06-13-2013 |
20130157324 | IN SITU EXPRESSION OF LIPASE FOR ENZYMATIC PRODUCTION OF ALCOHOL ESTERS DURING FERMENTATION - Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity. | 06-20-2013 |
20130157325 | Lipolytic Enzyme Variants - Molecular dynamics (MD) simulation on the three-dimensional structure of | 06-20-2013 |
20130164795 | CO-PRODUCTS FROM BIOFUEL PRODUCTION PROCESSES AND METHODS OF MAKING - The present invention includes methods of generating co-products for animal feed and compositions useful as co-products for animal feed derived from biofuel production processes. More specifically, the invention includes co-products for animal feed from at least one process feedstream, such as fatty acids from oil hydrolysis, lipids from evaporation of thin stillage, syrup, distillers grains, distillers grains and solubles, solids from a mash before fermentation, and solids from a whole stillage after fermentation. | 06-27-2013 |
20130164796 | SYSTEMS AND METHODS FOR CONTAMINANT REMOVAL FROM A MICROALGAE CULTURE - Systems and methods for the removal of contaminants from a liquid culture microalgae and/or cyanobacteria comprise an let tube, a pump, a gas injector, a vertical chamber, and/or a collection container that promotes the production of foam in the microalgae culture, wherein the foam contains the contaminants. | 06-27-2013 |
20130164797 | PROCESS FOR THE IMPROVED SEPARATION OF A HYDROPHOBIC ORGANIC SOLUTION FROM AN AQUEOUS CULTURE MEDIUM - A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyses one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof. | 06-27-2013 |
20130164798 | PROCESSES FOR PRODUCING HYDROCARBON PRODUCTS - The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels. | 06-27-2013 |
20130164799 | Integrated Process for the Production of Oil Bearing Chlorella Variabilis for Lipid Extraction Utilizing by Products of Jatropha Methyl Ester (JME) Production - An energy efficient process for the preparation of marine microalgae | 06-27-2013 |
20130171702 | METHOD FOR CULTURING MIXOTROPHIC UNICELLULAR ALGAE IN THE PRESENCE OF A DISCONTINUOUS SUPPLY OF LIGHT IN THE FORM OF FLASHES - A novel method for culturing mixotrophic single-cell algae makes it possible to enrich the lipid content of these algae, the enrichment being induced by a variable or discontinuous provision of light, in particular in the form of flashes. | 07-04-2013 |
20130189749 | Compositions, Methods, and Kits for Polyunsaturated Fatty Acids from Microalgae - The present invention provides compositions, methods, and kits comprising PUFAs produced by microalgae, in particular omega-3 and/or omega-6 fatty acids produced by members of the genus | 07-25-2013 |
20130196390 | METHOD OF PRODUCING BIOFUEL, BIODIESEL, AND OTHER VALUABLE CHEMICALS - a method for producing a hydrocarbon based product (e.g., biofuel or biodiesel), a microorganism (e.g., algae) that can be cultivated and harvested for producing a hydrocarbon based product, a method for producing a hydrocarbon based product from a by-product of biodiesel production, and a microorganism that can be cultivated from a by-product of biodiesel production and harvested for producing hydrocarbon based product. | 08-01-2013 |
20130196391 | PRODUCTION OF FATTY ACIDS BY HETEROLOGOUS EXPRESSION OF GENE CLUSTERS FROM MYXOBACTERIA - The invention relates to a process for producing one or more polyunsaturated fatty acids by means of heterologous gene expression comprising the steps of providing a production organism which comprises a heterologous gene cluster encoding a polyunsaturated fatty acid biosynthetic pathway encompassing a subsequence ER encoding an enoylreductase and a subsequence AT encoding an acyltransferase, growing the production organism in the presence of a fermentable carbon source whereby one or more polyunsaturated fatty acids are produced, and optionally recovering the one or more polyunsaturated fatty acids. | 08-01-2013 |
20130196392 | MICROALGAE BIOFUEL PRODUCTION SYSTEM - A system for processing oil from algae is disclosed. Specifically, the system involves the use of a consortium of algae strains as its input, wherein each algae strain has a unique characteristic for resisting/dominating a particular operational/environmental factor. Also, the system recycles byproducts of the process for use as nutrients during algae growth and oil production. The system includes a conduit for growing algae and an algae separator that removes the algae from the conduit. Also, the system includes a device for lysing the algae and an oil separator to remove the oil from the lysed matter. Further, the system includes a biofuel reactor that receives oil from the oil separator and synthesizes biofuel and glycerin. Moreover, the algae separator, oil separator and biofuel reactor all recycle byproducts back to the conduit to support further algae growth. | 08-01-2013 |
20130196393 | PROCESS FOR SEPARATING POLYUNSATURATED FATTY ACIDS FROM LONG CHAIN UNSATURATED OR LESS SATURATED FATTY ACIDS - A process for separating polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) and eicosapen-taenoic acid (EPA) from less saturated long chain fatty acids (LCFAs) in a lipid composition, wherein said PUFAs and LCFAs are present as (i) triglycerides, or (ii) free fatty acids or monoalkyl esters, by exchange of at least a portion of LCFAs with short and/or medium chain fatty acids (MCFAs). The process can suitably be employed on marine derived oil, marine oil 2 derived oil products and other sources of PUFAs, including PUFA-rich singe cell oils (SCOs), and oils from genetically modified organisms with a modified lipid metabolism. The inventive process is based on novel use of lipases and distillation techniques, selectively chemically modifying species in the substrate material such that the desired species and chemically similar species become sufficiently dissimilar to be separable. Thus PUFA can be effectively enriched from material such as 30 herring oil with low PUFA content and high contant of equal length monounsaturated fatty acids such as 20:1 and 22:1 fatty acids. | 08-01-2013 |
20130196394 | COMPOSITIONS AND METHODS FOR BACTERIAL LYSIS AND NEUTRAL LIPID PRODUCTION - A cyanobactenum that produces neutral lipids or alkanes, such as FAME OR FAEE, which may be used for biofuel production. | 08-01-2013 |
20130203133 | BUOYANT TRIACYLGLYCEROL-FILLED GREEN ALGAE AND METHODS THEREFOR - Cultures of | 08-08-2013 |
20130210093 | METHODS FOR PRODUCTION OF ALGAE DERIVED OILS - The present invention comprises a method of producing algae derived oils comprising growing, stressing, and harvesting algae and then extracting total lipids from which specific oil fractions are purified. | 08-15-2013 |
20130210094 | HETEROGENEOUS ENZYMATIC CATALYST, PROCESS FOR PREPARING SAME AND USE FOR CONTINUOUS FLOW ENZYMATIC CATALYSIS - The present invention relates to a heterogeneous enzymatic catalyst consisting of a macroprous silica monolith incorporating an enzyme immobilized by means of a compiling agent, to a process for preparing this enzymatic catalyst, to the use of the catalyst for carrying out chemical reactions by continuous flow heterogeneous enzymatic catalyst and to a process of continuous flow heterogeneous enzymatic catalysis using said catalyst. | 08-15-2013 |
20130210095 | NOVEL STRAINS OF MICROALGAE OF THE GENUS BOTRYOCOCCUS AND METHOD FOR THE CULTURE OF SAID MICROALGAE IN MIXOTROPHIC MODE - Novel strains of microalgae which belong to the | 08-15-2013 |
20130217082 | Algae Biofuel Carbon Dioxide Distribution System - A closed-loop system for growing algae in a bioreactor is disclosed, for example, to produce biofuel. An aqueous bioreactor solution is formulated such that the principal source of carbon for algae growth is supplied by sodium bicarbonate. During algae growth in the aqueous solution, the concentration of sodium bicarbonate in the solution is reduced while the concentration of sodium carbonate increases. A regenerator is provided to regenerate sodium bicarbonate from the sodium carbonate. Specifically, after sufficient growth, the algae are concentrated and an algae depleted media is produced. Carbon dioxide is then introduced into the algae depleted media to regenerate sodium bicarbonate from the sodium carbonate. The regenerated sodium bicarbonate is then recycled into the bioreactor to supply carbon for further algae growth. | 08-22-2013 |
20130217083 | GERMINATING ZYGOSPORES OF UNICELLULAR, SPORE-FORMING ALGAE AS A SOURCE OF ABUNDANT EXTRACTABLE LIPID - Described are procedures that promote the accumulation and release of intracellular lipid bodies from zygospores of single-celled alga. This disclosure describes procedures for manipulating the progression or synchronization of the algae's life cycle, and for simplifying lipid extraction by promoting spontaneous release of intracellular lipid reserves. The approach is relevant to the commercial production of biodiesel and food supplements derived from fatty acids. | 08-22-2013 |
20130217084 | PRODUCTION OF OMEGA-3 FATTY ACIDS FROM CRUDE GLYCEROL - The present invention relates to various methods to produce a variety of omega-3 fatty acids from various species of alga using crude glycerol as a substrate for algal growth. In one embodiment, the present invention relates to various methods to produced docosahexaenoic acid (DHA) from a | 08-22-2013 |
20130217085 | METHODS FOR IMPROVING FERMENTATION YIELD OF POLYUNSATURATED FATTY ACIDS - The present invention discloses a method of improving the fermentation yield of polyunsaturated fatty acids, in which | 08-22-2013 |
20130224810 | ENHANCED PRODUCTION OF FATTY ACIDS AND FATTY ACID DERIVATIVES BY RECOMBINANT MICROORGANISMS - The present invention provides for the improved production of fatty acid products in a recombinant microorganism or host cell engineered to express a non-native gene encoding a thioesterase and a non-native gene encoding a lysophosphatidic acid acyltransferase (LPAAT), in which the LPAAT is overexpressed in the recombinant microorganism compared to an otherwise identical microorganism that does not include the non-native LPAAT gene. The invention also provides methods of producing fatty acid products using such recombinant microorganisms. | 08-29-2013 |
20130224811 | FOUR-GENE PATHWAY FOR WAX ESTER SYNTHESIS - The invention relates to methods for producing a wax ester in recombinant host cells engineered to express a thioesterase, an acyl-CoA synthetase, an alcohol-forming fatty acyl reductase, and a wax ester synthase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, recombinant host cells expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, and systems for producing a wax ester via a pathway using these four enzymes, are also provided. | 08-29-2013 |
20130230891 | PEROXISOME BIOGENESIS FACTOR PROTEIN (PEX) DISRUPTIONS FOR ALTERING POLYUNSATURATED FATTY ACIDS AND TOTAL LIPID CONTENT IN OLEAGINOUS EUKARYOTIC ORGANISMS - Methods of increasing the amount of polyunsaturated fatty acids (PUFAs) in the total lipid fraction and in the oil fraction of PUFA-producing, oleaginous eukaryotes, accomplished by modifying the activity of peroxisome biogenesis factor (Pex) proteins. Disruptions of a chromosomal Pex3 gene, Pex10p gene or Pex16p gene in a PUFA-producing, oleaginous eukaryotic strain resulted in an increased amount of PUFAs, as a percent of total fatty acids and as a percent of dry cell weight, in the total lipid fraction and in the oil fraction of the strain, as compared to the parental strain whose native Pex protein was not disrupted. | 09-05-2013 |
20130236936 | A SYSTEM AND METHOD FOR SEPARATING HIGH VALUE BY-PRODUCTS FROM GRAINS USED FOR ALCOHOL PRODUCTION - Systems and methods are provided for separating high value by-products, such as oil and/or germ, from grains used for alcohol production. In one embodiment, a method for separating by-products from grains used for alcohol production includes, subjecting milled grains to liquefaction to provide a liquefied starch solution including fiber, protein, and germ. The germ is separated from the liquefied starch solution. The separated germ is ground, e.g., to a particle size less than 50 microns, to release oil to provide a germ/oil mixture. Then, prior to fermentation, the oil is separated from the germ/oil mixture to yield an oil by-product. The pH of the germ/oil mixture can be adjusted to about 8 to about 10.5 and/or cell wall breaking enzymes or chemicals may be added to help release oil from the germ. In one example, the oil yield is greater than 1.0 lb/Bu. | 09-12-2013 |
20130236937 | Algal Thermoplastics, Thermosets, Paper, Adsorbants and Absorbants - Provided are biomass-based materials and valuable uses of microalgal biomass including: (i) acetylation of microalgal biomass to produce a material useful in the production of thermoplastics; (ii) use of triglyceride containing microalgal biomass for production of thermoplastics; (iii) combination of microalgal biomass and at least one type of plant polymer to produce a material useful in the production of thermoplastics; (iv) anionization of microalgal biomass to form a water absorbant material; (v) cationization of microalgal biomass, and optional flocculation, to form a water absorbant material; (vi) crosslinking of anionized microalgal biomass; (vii) carbonization of microalgal biomass; and (viii) use of microalgal biomass in the making of paper. | 09-12-2013 |
20130236938 | BIODIESEL FUEL PRODUCTION, SEPARATION METHODS AND SYSTEMS - The present invention provides for a method for treatment of fats, oils and grease (FOG) for separation of target products and/or the efficient production of biodiesel while reducing disposal of solid or liquid waste matter into landfills or water treatment facilities. | 09-12-2013 |
20130252291 | Processing Of Oils and Fats - The invention relates to processing of oils and fats comprising applying an immobilized lipolytic enzyme, wherein a liquid aqueous lipolytic enzymes preparation is added to the immobilized lipolytic enzyme between feedstock batches, and/or is added to the immobilized lipolytic enzyme step wise or continuously during feedstock processing. | 09-26-2013 |
20130260427 | EXPRESSION OF CYTOSOLIC MALIC ENZYME IN TRANSGENIC YARROWIA TO INCREASE LIPID PRODUCTION - Transgenic | 10-03-2013 |
20130260428 | PROCESS FOR PREPARING BIODIESEL FROM RENEWABLE GREASE WITH LIPASE AS CATALYST AND ONLINE DEHYDRATION - Disclosed is a process for preparing biodiesel from renewable oil and fat catalyzed by lipase with online dehydration. The method uses a short chain alcohol ROH as an acyl-acceptor, and utilizes a lipase as the catalyst to catalyze transesterification reaction of oil and fat feedstock with said short chain alcohol, so as to synthesize biodiesel; and online dehydration with a membrane or a molecular sieve is carried out during the whole process or part of the reaction process. Such online dehydration process greatly simplifies the operation procedure, and provides good economic and environmental benefits. | 10-03-2013 |
20130273620 | METHODS FOR ALTERING LIPIDS IN ALGAE AND YEAST - The present disclosure relates to methods of increasing lipid levels in an algal or yeast cell population, methods of producing saturated or monounsaturated triacylglycerols in an algal or yeast cell population, and methods of decreasing polyunsaturated triacylglycerol production in an algal or yeast cell population by contacting the cell population with a chemical compound that is capable of increasing lipid levels or altering the lipid composition in the cell population. | 10-17-2013 |
20130273621 | Recombinant Microalgae Cells Producing Novel Oils - Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus. | 10-17-2013 |
20130280770 | Transgenic Algae with Enhanced Oil Expression - The present invention provides transgenic algal cells that produce an increased amount of oil, methods of making transgenic algal cells, and methods of obtaining biofuel from the transgenic algal cells. | 10-24-2013 |
20130280771 | METHOD FOR PRODUCING BIODIESEL - The present disclosure is directed to the method for producing the biodiesel comprising steps of providing a biomass having an oil, obtaining a crude mixture of the biomass, and then performing a transesterification in which the crude mixture is used as a reacting material thereof to produce the biodiesel. | 10-24-2013 |
20130295623 | BICARBONATE TRIGGER FOR INDUCING LIPID ACCUMULATION IN ALGAL SYSTEMS - The present invention provides bicarbonate containing and/or bicarbonate-producing compositions and methods to induce lipid accumulation in an algae growth system, wherein the algae growth system is under light-dark cycling condition. By adding said compositions at a specific growth stage, said methods lead to much higher lipid accumulation and/or significantly reduced total time required for accumulating lipid in the algae growth system. | 11-07-2013 |
20130295624 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. | 11-07-2013 |
20130302863 | COMPOSITIONS CONTAINING HIGH OMEGA-3 AND LOW SATURATED FATTY ACID LEVELS - Food products and supplements comprising the essential unsaturated fatty acids EPA or DHA at high concentrations relative to other n-3 HUFAs and with relatively low levels of saturated fatty acids (myristic and palmitic acids) are provided. These compositions can be made in commercial quantities in a cost effective manner by culture of selected microorganisms. The food products and supplements are suited to human health needs which are unable to be met from fish oil origins. Also provided are EPA containing food products obtained from animals. | 11-14-2013 |
20130302864 | Method for Producing a Fatty Acid Ester - A method for producing a fatty acid ester, which comprises: (a) incubating a culture of microalga at a mid-temperature, wherein the culture is obtained by culturing the microalga in a medium, (b) then adding an alcohol, allowing a reaction at a temperature lower than the mid-temperature, and (c) collecting a fatty acid ester from an obtained reaction product. | 11-14-2013 |
20130316410 | Tailored Oils - Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type. | 11-28-2013 |
20130323801 | Compositions, Methods, and Kits for Polyunsaturated Fatty Acids from Microalgae - The present invention provides compositions, methods, and kits comprising PUFAs produced by microalgae, in particular omega-3 and/or omega-6 fatty acids produced by members of the genus | 12-05-2013 |
20130323802 | METHOD FOR PRODUCING OIL BY YEAST - A method for producing oil in a form of triglyceride is provided. The method comprises steps of providing a carbon source and a nitrogen source; and culturing a yeast strain of | 12-05-2013 |
20130323803 | METHODS AND COMPOSITIONS FOR THE PRODUCTION OF EXTREMOPHILE ENZYMES FROM GREEN MICROALGAE AND CYANOBACTERIA - The present invention relates to compositions and methods for stable transformation of green microalgae and for production of transgenic green microalgae and/or cyanobacteria that produce extremophile enzymes as co-products during the growth of the green microalgae and/or cyanobacteria for lipid biofuel production. Thus, the present invention provides nucleic acid constructs and methods of transformation useful in the production of stably transformed green microalgae and/or cyanobacteria expressing extremophile enzymes in combination with lipid production for biofuel. | 12-05-2013 |
20130330790 | Renewable Chemicals and Fuels from Oleaginous Yeast - The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims. | 12-12-2013 |
20130337514 | PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES - Acyl-CoA:lysophosphatidylcholine acyltransferase [“LPCAT”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) is disclosed herein to be over-expressed along with the over-expression of phospholipid:diacylglycerol acyltransferase [“PDAT”] having the ability to transfer a fatty acyl group from the sn-2 position of a phospholipid (e.g., phosphatidylcholine) to the sn-3 position of 1,2-diacylglycerol [E.C.2.3.1.158], thus resulting in a lysophospholipid and TAG. Co-expression of these enzymes in a recombinant microbial host cell resulted in increased production of long chain polyunsaturated fatty acids [“PUFAs”]. | 12-19-2013 |
20130337515 | PROCESS FOR PRODUCTION OF LOW SATURATE OILS - Provided here is an enzymatic process for production of low saturate oil, in one embodiment, low palmitic oils from triacylglycerol sources. The enzymes used in the processes herein are saturase enzymes, including palmitase enzymes. The oils produced by the processes herein are used in food products. | 12-19-2013 |
20130344546 | EUKARYOTIC MICROORGANISMS FOR PRODUCING LIPIDS AND ANTIOXIDANTS - Disclosed are compositions and methods related to eukaryotic microorganisms that can produce unsaturated fatty acids which can be purified and used. | 12-26-2013 |
20130344547 | RECOMBINANT MICROORGANISMS MAKE BIODIESEL - A carboxydotrophic acetogenic recombinant microorganism is modified so that it produces biodiesel and optionally one or more other products by fermentation of a substrate comprising CO. Biodiesel is produced by microbial fermentation of a substrate comprising CO. The recombinant microorganism is modified to express one or more exogenous enzymes in the biodiesel biosynthesis pathway not present in a parental microorganism from which the recombinant microorganism is derived. The one or more enzymes comprise a nonspecific acyltransferase. | 12-26-2013 |
20130344548 | ENGINEERED MICROBES AND METHODS FOR MICROBIAL OIL OVERPRODUCTION FROM CELLULOSIC MATERIALS - The invention relates to engineering microbial cells for utilization of cellulosic materials as a carbon source, including xylose. | 12-26-2013 |
20130344549 | MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING LIPIDS - This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl carrier protein (ACP), an acyl-ACP synthase (Aas), or both, optionally in combination with one or more overexpressed or exogenous lipid biosynthesis proteins, and/or one or more overexpressed or exogenous glycogen breakdown proteins. Exemplary biosynthesis proteins include diacyglycerol acyltransferases, thioesterases, phosphatidate phosphatases, phospholipases, triacylglycerol (TAG) hydrolases, fatty acyl-CoA synthetases, and/or acetyl-CoA carboxylases, including combinations thereof. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids and/or synthesizing triglycerides. | 12-26-2013 |
20140004578 | MICROBES, METHODS, AND DEVICES FOR REDOX-IMBALANCED METABOLISM | 01-02-2014 |
20140004579 | PROCESS FOR SEPARATION OF RENEWABLE MATERIALS FROM M ICROORGANISMS | 01-02-2014 |
20140004580 | MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING LIPIDS | 01-02-2014 |
20140004581 | IMMOBILIZED INTERFACIAL ENZYMES OF IMPROVED AND STABILIZED ACTIVITY | 01-02-2014 |
20140011246 | Methods for Harvesting and Processing Biomass - A system and method for harvesting and processing algae, the system and method including harvesting algae by mechanical or chemical system and processing the harvested algae to produce at least one of biodiesel, biosolvents, bioplastics, biogas, or fertilizer. | 01-09-2014 |
20140011247 | SYNTHESIS OF LONG-CHAIN POLYUNSATURATED FATTY ACIDS BY RECOMBINANT CELL - The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. | 01-09-2014 |
20140017741 | Esterification Process - The invention relates to the utilisation of fatty acid feedstockin the production of biodiesel by the use of microbial enzymes. | 01-16-2014 |
20140017742 | PREPARATION OF MICROBIAL OIL - The present invention provides a process for the production of a microbial oil comprising culturing a micro-organism in a two stage fermentation process where, in a last stage that precedes the end of fermentation, the carbon source is: consumed by the micro-organisms at a rate greater than it is added to the medium; added at a rate # 0.30 M carbon/kg medium; or is rate limiting on the growth of the micro-organism. The micro-organisms thus have the carbon source restricted so that they preferentially metabolise fats or lipids other than arachidonic acid (ARA), so increasing the proportion of ARA in the cells. A microbial oil is then recovered from the micro-organism, using hexane as a solvent, that has at least 50% ARA and at least 90% triglycerides. | 01-16-2014 |
20140030771 | COMPOSITIONS AND METHODS FOR INCREASING OIL PRODUCTION AND SECRETION - The present invention provides compositions and methods related to the production of fatty acids, such as triglycerides with genetically engineered cells, such as algae. | 01-30-2014 |
20140030772 | HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF YARROWIA LIPOLYTICA - Engineered strains of the oleaginous yeast | 01-30-2014 |
20140038247 | ALGAE HAVING INTRACELLULAR LIPID PARTICLES AND HIGH LIPID CONTENT - A method for increasing the lipid content of algae includes combining algae and a lipid-precipitating addition in a growth-inhibitive medium, the growth-inhibitive medium being deficient in at least one nutrient that is necessary for reproductive growth of algae, thus frustrating algae reproduction, the lipid-precipitating addition selected from hydroxyl-containing compounds and amine-containing compounds. An organic acid addition may also be combined with the lipid-precipitating addition and algae in the growth-inhibitive medium, the organic acid addition including compounds containing carboxylic acid functionality. Direct production of biodiesel is also achieved by the use of particular lipid-precipitating additions and organic acid additions. | 02-06-2014 |
20140038248 | PRODUCT OF FATTY ACID ESTERS FROM BIOMASS POLYMERS - The invention provides consolidated bioprocessing methods and host cells. The host cells are capable of directly converting biomass polymers or sunlight into biodiesel equivalents and other fatty acid derivatives. In particular, the invention provides a method for producing biodiesel equivalents and other fatty acid derivatives from a biomass polymer including providing a genetically engineered host cell, culturing the host cell in a medium containing a carbon source such that recombinant nucleic acids in the cell are expressed, and extracting biodiesel equivalents and other fatty acid derivatives from the culture. | 02-06-2014 |
20140045229 | TWO-STAGE PROCESS FOR PRODUCING OIL FROM MICROALGAE - A process for production of biofuels from algae can include cultivating an oil-producing algae by promoting sequential photoautotrophic and heterotrophic growth. The method can further include producing oil by heterotrophic growth of algae wherein the heterotrophic algae growth is achieved by introducing a sugar feed to the oil-producing algae. An algal oil can be extracted from the oil-producing algae, and can be converted to form biodiesel. | 02-13-2014 |
20140051136 | Micoorganisms and Methods for the Production of Fatty Acids and Fatty Acid Derived Products - This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism | 02-20-2014 |
20140073021 | PROCESSES AND SYSTEMS FOR THE PRODUCTION OF FERMENTATION PRODUCTS - The present invention relates to the production of fermentation products such as alcohols including ethanol and butanol, and processes employing in situ product removal methods. | 03-13-2014 |
20140093921 | BACTERIA AND METHOD FOR SYNTHESIZING FATTY ACIDS - There is provided a recombinant bacterium comprising at least one overexpressed acyl-ACP thioesterase gene, and wherein at least one gene from the tricarboxylic acid cycle or glycolysis or both is inactivated. There is also provided a method for producing fatty acids, said method comprising culturing bacteria comprising at least one overexpressed acyl-ACP thioesterase gene in a growth medium in a container having walls; allowing said bacteria to secrete fatty acids; and collecting said fatty acids. Acid supplementation is also shown to increase productivity. | 04-03-2014 |
20140093922 | MICROBIAL CULTURE MEDIUM AND CULTURAL METHOD - The present invention discloses a microbial culture medium and a cultural method. The culture medium comprises a combination of strong acid-weak alkali salts and strong alkali-weak acid salts so that pH value thereof can be regulated and controlled in a stable and suitable range by itself, thus decreasing or eliminating an operable regulation on pH value via an external device in fermentation process and a risk of contaminations resulted from it in fermentation process as well, accordingly greatly improving the controllability and stability of the fermentation process. Meanwhile the ammonium salts acting as strong acid-weak alkali salts can be used as a nitrogen source to replace yeast cream to realize a decreased amount of nitrogen resource of high price, therefor sharply reducing the cost of the culture medium. | 04-03-2014 |
20140093923 | Methods and Compositions for the Extracellular Transport of Biosynthetic Hydrocarbons and Other Molecules - The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into hydrocarbons, e.g., n-alkanes and n-alkenes, wherein the n-alkanes are secreted into the culture medium via recombinantly expressed transporter proteins. In particular, the use of such organisms for the commercial production of n-alkanes and related molecules is contemplated. | 04-03-2014 |
20140099683 | Omega-3 desaturase used in the biosynthesis of polyunsaturated fatty acids - The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially omega-3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases. | 04-10-2014 |
20140099684 | ENGINE WORTHY FATTY ACID METHYL ESTER (BIODIESEL) FROM NATURALLY OCCURING MARINE MICROALGAL MATS AND MARINE MICROALGAE CULTURED IN OPEN SALT PANS TOGETHER WITH VALUE ADDITION OF CO-PRODUCTS - The invention teaches the obtained specifications and process of production of engine worthy marine microalgal fatty acid methyl ester (biodiesel) using naturally occurring marine microalgal mats and also marine microalgae cultivated in cost-effective manner in solar salt pans. Utility of co-product streams adds to the attractiveness of the invention. | 04-10-2014 |
20140099685 | BIOREACTORS APPARATUS, SYSTEM AND METHOD - The present invention provides a bioreactor having a reactor chamber and one or more support chambers. The reactor chamber can have one or more flexible walls for enclosing microorganisms and culture medium. The reactor chamber provides an enclosure for microorganism and culture medium. The support chamber can also have one or more flexible walls. When inflated to a predetermined amount, the support chamber causes the microorganisms and culture medium to distribute to a substantially even depth across the reactor chamber. | 04-10-2014 |
20140106417 | DGAT GENES AND METHODS OF USE FOR TRIGLYCERIDE PRODUCTION IN RECOMBINANT MICROORGANISMS - The present invention provides novel diacylglycerol acyltransferase (DGAT) genes, including novel genes encoding localization peptides. The present invention also provides recombinant cells, such as algae, transformed with DGAT genes and methods of using such recombinant cells to produce triglyceride. | 04-17-2014 |
20140106418 | Enhanced Fermentation From Pretreatment Products - Provided are methods and compositions for higher yields through the addition of pretreatment/hydrolysis extracts in the fermentation process. These fractions increase the rate of growth for biocatalysts and result in improved composition of fermentation end products. | 04-17-2014 |
20140106419 | PROCESSES AND SYSTEMS FOR THE PRODUCTION OF FERMENTATION PRODUCTS - The present invention relates to processes and systems for the production of fermentation products such as alcohols. The present invention also provides methods for separating feed stream components for improved biomass processing and productivity. | 04-17-2014 |
20140113340 | HIGH SALINITY TOLERANT MICROALGAE STRAINS, PRODUCTS DERIVED THEREFROM, AND METHODS OF PRODUCING THE SAME - The present invention relates generally to microalgae strains that are tolerant to high salinity, the products derived from the high salinity tolerant microalgae strains, and methods of producing high salinity tolerant microalgae strains and their products. | 04-24-2014 |
20140120589 | Fatty Acid Esterification Process - The invention relates to the utilisation of fatty acid feedstocks with substantial free fatty acid content in the production of biodiesel by the use of microbial enzymes. | 05-01-2014 |
20140120590 | NOVEL STRAIN OF MICROALGAE OF THE ODONTELLA GENUS FOR THE PRODUCTION OF EPA AND DHA IN MIXOTROPHIC CULTIVATION MODE - A novel strain of microalga of the | 05-01-2014 |
20140127765 | MICROORGANISMS AND METHODS FOR PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS AND RELATED COMPOUNDS - The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid. | 05-08-2014 |
20140127766 | SYSTEM FOR OBTAINING BIOMASS - This invention is a system for the production of biomass from photosynthesizing microorganisms that includes a photobioreactor comprising a transparent panel made from two transparent sheets with a separation between them, with top and bottom openings and with transparent, parallel subdivisions that form a panel of vertically arranged, transparent cells, where each transparent cell has a top opening and a bottom opening; a lower recirculation chamber in fluid contact with the bottom openings of the transparent panel; an upper recirculation chamber in fluid contact with the top openings of the transparent panel; a gas distribution tube externally arranged along the edge of said transparent panel; where said gas distribution tube comprises gas injectors arranged in fluid contact with the interior of a plurality of transparent cells; and a supporting structure that supports the transparent panel, the lower recirculation chamber, the upper recirculation chamber and the air distribution tube. | 05-08-2014 |
20140134684 | Methods For Obtaining Oil From Maize Using Acid Protease and Cell-wall Polysaccharide-degrading Enzymes - Disclosed are methods for obtaining oil from maize, involving grinding maize kernels to form flour, adding water to the flour to form a slurry, and incubating the slurry with α-amylase for about 10 minutes to about 180 minutes at a temperature of about 75° to about 120° C. and at a pH of about 3 to about 7 to form a mash, cooling the mash to about 15° C. to about 40° C. and adding a nitrogen source, glucoamylase, yeast, acid protease, and cell-wall polysaccharide-degrading enzymes to form a beer containing ethanol and oil, wherein the beer has a pH of about 3 to about 7, and recovering oil from the beer. | 05-15-2014 |
20140134685 | METHOD TO INCREASE ALGAL BIOMASS AND ENHANCE ITS QUALITY FOR THE PRODUCTION OF FUEL - The invention provides a recombinant cell having a nucleotide sequence encoding a polypeptide which is a lipase having at least 40% amino acid sequence identity to a polypeptide having SEQ ID NO:1, and methods of using the recombinant cell to produce triacylglycerols or to increase oil production by the cell. | 05-15-2014 |
20140141477 | FERMENTATION PROCESS - The invention provides methods and systems for the production of lipid products from a gaseous substrate using a two stage fermentation process. The method comprises providing a gaseous substrate comprising CO, CO | 05-22-2014 |
20140147897 | TWO STAGE HIGH SPEED CENTRIFUGES IN SERIES USED TO RECOVER OIL AND PROTEIN FROM A WHOLE STILLAGE IN A DRY MILL PROCESS - Methods of and devices for recovering oil and protein and increasing production yields using two centrifuges in series or a centrifuge with dual oil and protein separating functions in a dry mill are provided. | 05-29-2014 |
20140154755 | FERMENTATION PROCESS - The invention provides methods and systems for the production of lipid products from a gaseous substrate using a two stage fermentation process. The method comprises providing a gaseous substrate comprising CO or CO | 06-05-2014 |
20140162328 | Efficient production of biofuels from cells carrying a metabolic-bypass gene cassette - A process for increasing the production of a glycolytic intermediate and/or an organic compound as defined herein by a cell that is able to express a nucleic acid molecule, wherein the expression of the nucleic acid molecule gives the cell the ability to increase the production of a glycolytic intermediate and/or the organic compound and wherein the carbon substrate is sugar and/or protein. | 06-12-2014 |
20140162329 | DGAT GENES COMPRISING PLECKSTRIN HOMOLOGY DOMAINS AND METHODS OF USE FOR TRIGLYCERIDE PRODUCTION IN RECOMBINANT MICROORGANISMS - The present invention provides novel diacylglycerol acyltransferase (DGAT) genes comprising Pleckstrin Homology (PH) domains. The present invention also provides for recombinant cells, such as algae, transformed with acyltransferase genes, such as DGAT, comprising PH domains, and methods of using such recombinant cells to produce increased triglyceride levels. | 06-12-2014 |
20140162330 | ENGINEERED MICROALGAE WITH ENHANCED LIPID PRODUCTION - Described herein are engineered microalgae that exhibit enhanced lipid production during exponential growth. Such engineered microalgae are useful, for example, for the production of biofuels. | 06-12-2014 |
20140162331 | AMPHIPATHIC PEPTIDE-LIPASE CONJUGATE HAVING ADVANCED LIPASE ACTIVITY AND USE THEREOF - Disclosed are an amphipathic peptide-lipase conjugate with enhanced lipase activity, a polynucleotide coding for the conjugate, an expression vector carrying the polynucleotide, a transformant anchoring the expression vector therein, a method for preparing the conjugate, a lipolysis method using the conjugate, and a method for producing biodiesel using the lipase. | 06-12-2014 |
20140170716 | Renewable Diesel and Jet Fuel from Microbial Sources - The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel. | 06-19-2014 |
20140178950 | GENETICALLY ENGINEERED MICROBIAL STRAINS INCLUDING CHLORELLA PROTOTHECOIDES LIPID PATHWAY GENES - Genetically engineered microbial, e.g., | 06-26-2014 |
20140178951 | SYSTEM AND PROCESS FOR OBTAINING PRODUCTS FROM BIOMASS - A process for producing products from biomass comprising fermenting biomass to produce a first product stream comprising carboxylic acid salts; acidifying at least one of the first product stream and a second product stream to produce a third product stream comprising acids; extracting such acids from the third product stream with a solvent; separating the extracting solvent from the acids to produce the separated extracting solvent and a fourth product stream comprising acids; and processing the fourth product stream to produce a fifth product stream. | 06-26-2014 |
20140186906 | RECOMBINANT MICROBIAL CELLS THAT PRODUCE AT LEAST 28% EICOSAPENTAENOIC ACID AS DRY CELL WEIGHT - Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA. | 07-03-2014 |
20140186907 | OIL COMPOSITION AND METHOD OF RECOVERING SAME - The present invention generally relates to oil compositions and methods of producing such oil compositions. More particularly, the present invention relates to an oil composition recovered from a fermentation product as well as methods of recovering such oil compositions for use in various processes such as bio-diesel production as well as in various edible applications. | 07-03-2014 |
20140186908 | ENZYMATIC TRANSESTERIFICATION/ESTERIFICATION PROCESSES EMPLOYING LIPASES IMMOBILIZED ON HYDROPHOBIC RESINS IN THE PRESENCE OF WATER SOLUTIONS - Disclosed are processing systems and processes for carrying out enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food and detergent industries. | 07-03-2014 |
20140186909 | Flexible Photobioreactors, Systems and Methods - The present invention provides a photobioreactor for a phototrophic microorganism, and culture medium therefor, comprising a capsule, the capsule comprising an elongated body comprised of a first, flexible polymer film that is at least partially transparent to light of a wavelength that is photosynthetically active in a phototrophic microorganism and divided width wise into a plurality of adjacent channels, each channel having a major cross-sectional dimension that is not more than about one-third of the length of the elongated body, when the elongated body is inflated; and a fluid distribution structure coupled to the elongated body adapted for fluid communication with the plurality of adjacent channels that distributes a flow of culture medium amongst the plurality of channels, wherein the fluid distribution structure is comprised of a second, flexible polymer film. | 07-03-2014 |
20140193866 | Compositions and Methods for Making and Modifying Oils - The invention provides novel methods for making or modifying oils, e.g., plant animal or microbial oils, such as vegetable oils or related compounds, that are low in a particular fatty acid(s), for example, low linoleic oils, linolenic oils, low palmitic oils, low stearic oils or oils low in a combination thereof. | 07-10-2014 |
20140193867 | MICROBIAL ODD CHAIN FATTY ACIDS - Engineered bacteria that produce a predominance of odd-chain free fatty acids. | 07-10-2014 |
20140199738 | METHOD FOR PREPARING VOLATILE FATTY ACIDS FROM THE PRE-TREATED EXTRACTS OF MARINE BIOMASS RESIDUE - A method of preparing a volatile fatty acids (VFAs) is provided. More particularly, the method includes chemically or biologically pretreating a residue of algae to obtain an extract of the algae residue, filtering the extract of the algae residue and anaerobically fermenting the filtrate. The method of preparing VFAs can be used regardless of the kinds of algae, a process of producing VFAs can be simplified since there is no need to develop an enzyme for anaerobic fermentation, and a yield of VFAs can be increased since the VFAs can be produced from different kinds of organic components such as proteins or fats in addition to carbohydrates. Accordingly, the method can be useful in producing VFAs in an economical and efficient manner. | 07-17-2014 |
20140199739 | NOVEL STRAINS OF MICROALGAE OF THE ISOCHRYSIS GENUS FOR PRODUCING EPA AND DHA IN A MIXOTROPHIC MODE - Novel strains of microalgae belonging to the | 07-17-2014 |
20140199740 | WET OXIDATION OF BIOMASS - The present disclosure comprises methods, apparatus, components, and techniques for pretreatment of biomaterials using targeted wet oxidation. The targeted wet oxidation pretreatment is an upstream method for converting solid biomass into fuels and/or specialty chemicals. Embodiments of the present disclosure comprise methods carried out on biomaterials to selectively oxidize lignin components of the biomass, thereby resulting in bio accessible/digestible biomass fibers. In embodiments of the present disclosure, such methods may comprise pretreatment processes to prepare the biomaterials for a subsequent fermentation or other like conversion may be carried out to result in useful bio fuels or other bioproducts. | 07-17-2014 |
20140212935 | SHORT CHAIN FATTY ACIDS FROM BACTERIA - The present invention relates to an engineered bacteria for producing short chain fatty acid with the overexpression of a long chain (>C12) acyl-ACP thioesterases (long-TE) and a short chain (≦C12) acyl-ACP thioesterases (short-TE). | 07-31-2014 |
20140212936 | Solventless Extraction Process - The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained. | 07-31-2014 |
20140220645 | DOWN-REGULATION OF A POLYNUCLEOTIDE ENCODING A SOU2 SORBITOL UTILIZATION PROTEIN TO MODIFY LIPID PRODUCTION IN MICROBIAL CELLS - Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA. | 08-07-2014 |
20140220646 | APPARATUS FOR TREATING FOOD WASTE AND EXTRACTING BIO OIL - An apparatus for treating food waste and extracting bio oil includes: a fermentation dryer which ferments and decomposes food waste for 24 hours to reduce moisture contained in the food waste to 40 to 50%, a crusher which crushes the food waste into small particles, a distillation tank which heats the crushed food waste at low pressure and temperature so that oil is gasified by means of low temperature thermal decomposition to form oil gas, a distillation tower in which the oil gas is cooled to be liquefied and separated into moisture (carbon liquid fertilizer) and oil, a vacuum storage tank which collects the separated oil, and a centrifugal separator which rectifies the oil. The apparatus extracts a carbon liquid fertilizer, bio oils, and activated carbon while treating organic substances such as food waste and residual product compost. | 08-07-2014 |
20140234919 | SIMULTANEOUS SACCHARIFICATION AND FERMENTATION(SSF) OF LIGNOCELLULOSIC BIOMASS FOR SINGLE CELL OIL PRODUCTION BY OLEAGINOUS MICROORGANISMS - Methods and systems for producing lipids which may be used as biofuel are provided. Sugars produced by pretreatment of lignocellulosic biomass are utilized by heterotrophic oleaginous fungi or yeast to increase their biomass and to produce lipids without prior detoxification of the pretreated biomass. After the fungi/yeast are cultured with the sugars, solid residues from the pretreated biomass are combined with the fungi/yeast under conditions which allow simultaneous 1) enzymatic degradation of cellulose and/or hemicellulose to produce sugars and 2) fermentation of the sugars for further increases in biomass and lipid production. | 08-21-2014 |
20140234920 | VARIANT THIOESTERASES AND METHODS OF USE - The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein. | 08-21-2014 |
20140242648 | PROTEIN HAVING ACTIVITY TO PROMOTE FATTY ACID CHAIN ELONGATION, GENE ENCODING SAME AND USE THEREOF - The present invention relates to a protein having an activity to promote fatty acid chain elongation, a polynucleotide encoding the same, etc. The present invention provides, for example, a polynucleotide containing the nucleotide sequence shown in SEQ ID NO: 1 or 4, a polynucleotide encoding a protein which consists of the amino acid sequence shown in SEQ ID NO: 2, an expression vector and a transformant, each containing such a polynucleotide, a method for preparing lipids or fatty acids by using such a transformant, or a food or the like containing lipids or fatty acids prepared by such a method. | 08-28-2014 |
20140242649 | Hybrid organic-inorganic system for producing biofuels - The present invention provides for a system for converting CO | 08-28-2014 |
20140248674 | Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 09-04-2014 |
20140256006 | PROCESSES FOR PRODUCING HYDROCARBON PRODUCTS - The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels. | 09-11-2014 |
20140273112 | METHODS OF USING NATURAL AND ENGINEERED ORGANISMS TO PRODUCE SMALL MOLECULES FOR INDUSTRIAL APPLICATION - Aspects of the invention relate to methods of producing small molecules for industrial application using natural organisms and engineered organisms. | 09-18-2014 |
20140273113 | CHLOROPHYLL DEFICIENT ALGAL CELL WITH IMPROVED GROWTH AND PRODUCTION - Chlorophyll deficient algal strains, methods of making chlorophyll deficient algal strains, and methods of their use are provided. | 09-18-2014 |
20140273114 | BACTERIA AND METHOD FOR SYNTHESIZING FATTY ACIDS - The present invention discloses a process for increasing the production of free fatty acids at high yield (close to maximum theoretical yield), with various fatty acid compositions and various percentage of fatty acids accumulated intracellularly. This invention will enable the efficient production of other products derived from free fatty acids and/or products that can be branched out from the fatty acid synthesis pathways. | 09-18-2014 |
20140302569 | ALGAE STRAIN FOR BIODIESEL FUEL PRODUCTION - Use of an algal for biodiesel fuel selected for growing strain of production, the genus | 10-09-2014 |
20140302570 | METHOD AND SYSTEM FOR PROCESSING A BIOMASS FOR PRODUCING BIOFUELS AND OTHER PRODUCTS - The present invention provides methods of processing a biomass, e.g., for producing lipid and non-lipid materials, by inducing autolysis of a biomass. The biomass may be generated using any species of microorganism and any available biodegradable substrate, including waste materials. The lipid and non-lipid materials are present in two separate layers of the autolysate, and they can be used to generate valuable products such as biofuels and nutritional supplements. The present invention further provides a processing apparatus useful for practicing the methods of the present invention. | 10-09-2014 |
20140315265 | PROCESS OF PRODUCING OIL FROM ALGAE USING BIOLOGICAL RUPTURING - A process for production of biofuels from algae can include cultivating an oil-producing algae, extracting the algal oil, and converting the algal oil to form biodiesel. Extracting the algal oil from the oil-producing algae can include biologically rupturing cell wall and oil vesicles of the oil-producing algae using at least one enzyme such as a cellulose or glycoproteinase, a structured enzyme system such as a cellulosome, a virus, or combination of these materials. | 10-23-2014 |
20140315266 | ACCELERATED PROCESS FOR THE ENERGY CONVERSION OF CARBON DIOXIDE - The present invention relates to a process for the energy conversion of carbon dioxide, comprising the steps of culturing phytoplankton in electromagnetic bioaccelerators, producing oxygen and biomass made up of lipids, hydrocarbons and sugars from the previous step, oxidizing the hydrocarbons produced in the previous step to generate carbon dioxide and NOx and collecting the carbon dioxide and NOx from the previous step until the cultures of the first step. | 10-23-2014 |
20140315267 | Tailored Oils Produced from Recombinant Heterotrophic Microorganisms - Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants. | 10-23-2014 |
20140322771 | Lipid Production - The invention relates to methods for producing lipids and in particular methods for producing glycerolipids suitable for use in generating biofuels. The glycerolipids are produced through the modification of a cell so as to increase phosphatidic acid via the inhibition of the phosphatidylethanolamine N-methyltransferase (PEMT) pathway. The invention further relates to genetically modified plants and microorganisms in which the production of glycerolipids/oil is increased. | 10-30-2014 |
20140329287 | COMPOSITIONS AND METHODS FOR LIPID PRODUCTION - Described herein, inter alia, are compositions, oleagnious organisms, and methods useful for producing lipids, lipid precursors, and/or oleochemicals. | 11-06-2014 |
20140335578 | INTEGRATED BIODIESEL PROCESS - Methods of using crude glycerol to make fatty acids are provided, as well as integrated methods of converting glycerol waste from biodiesel production into more biodiesel. Bacteria and other microbes engineered to produce free fatty acids from glycerol are also provided. | 11-13-2014 |
20140335579 | DESATURASES AND PROCESS FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS - The present invention relates to polynucleotides from | 11-13-2014 |
20140335580 | Method for Enrichment of Eicosapentaenoic Acid and Docosahexaenoic Acid in Source Oils - An simpler, cheaper method for enhancing the percentage of eicosapentaenoic acid (EPA) and/or docosahexaenoic acid (DHA) from source oils has been discovered. This method hydrolyzes the oil using the lipase enzyme but does not inactivate the lipase enzyme with either added chemicals or increased temperature. The method relies on centrifugation to separate the enzyme from the desired oil and separate the oil from aqueous impurities. The hydrolyzed oil is enhanced with EPA and DHA, and can be further purified by using activated surface adsorbents, e.g., activated alumina, to remove more free fatty acids and impurities. | 11-13-2014 |
20140356917 | PROCESS FOR PRODUCING LIPIDS SUITABLE FOR BIOFUELS - The present invention provides a cost effective biotechnological process for production of bio-fuels from isolated and characterized microalgae. The algal strains used in the present invention having higher biomass, higher lipid productivity, higher pH and temperature tolerance are selected from the group consisting of | 12-04-2014 |
20140363860 | Omega-3 desaturase used in the biosynthesis of polyunsaturated fatty acids - The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially ω3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases. | 12-11-2014 |
20140370558 | MODIFYING SOYBEAN OIL COMPOSITION THROUGH TARGETED KNOCKOUT OF THE FAD2-1A/1B GENES - Materials and methods are provided for making soybean varieties that have altered oil composition as a result of mutations in the FAD2-1A and FAD2-1B genes. | 12-18-2014 |
20140377817 | HEAT STABLE, FE DEPENDENT ALCOHOL DEHYDROGENASE FOR ALDEHYDE DETOXIFICATION - The present invention relates to microorganisms and polypeptides for detoxifying aldehydes associated with industrial fermentations. In particular, a heat-stable, NADPH- and iron-dependent alcohol dehydrogenase was cloned from | 12-25-2014 |
20140377818 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, methods are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation. | 12-25-2014 |
20150010966 | ENZYMATIC PROCESS FOR FAT AND OIL HYDROLYSIS - An efficient process for enzymatic hydrolysis of fats and oils in a homogenous mixture is provided herein. The present invention in particular provides a process for production of fatty acids, sn-regio mono-acylglycerol (MAG), sn-regio di-acyl-glycerols (DAG), and glycerol from fats, wherein more than 98% fats can be converted into the desired product. The present invention also provides a process for the production of fatty acids and glycerol, virtually free of sn-regio diacyl-glycerols (DAG) and comprising less than 5% sn-regio mono-acylglycerol (MAG) in the end product. | 01-08-2015 |
20150017694 | Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same - Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins. | 01-15-2015 |
20150024441 | PEROXISOME BIOGENESIS FACTOR PROTEIN (PEX) DISRUPTIONS FOR ALTERING POLYUNSATURATED FATTY ACIDS AND TOTAL LIPID CONTENT IN OLEAGINOUS EUKARYOTIC ORGANISMS - Methods of increasing the amount of polyunsaturated fatty acids (PUFAs) in the total lipid fraction and in the oil fraction of PUFA-producing, oleaginous eukaryotes, accomplished by modifying the activity of peroxisome biogenesis factor (Pex) proteins. Disruptions of a chromosomal Pex3 gene, Pex10p gene or Pex16p gene in a PUFA-producing, oleaginous eukaryotic strain resulted in an increased amount of PUFAs, as a percent of total fatty acids and as a percent of dry cell weight, in the total lipid fraction and in the oil fraction of the strain, as compared to the parental strain whose native Pex protein was not disrupted. | 01-22-2015 |
20150024442 | MODIFIED DIACYLGLYCEROL ACYLTRANSFERASE PROTEINS AND METHODS OF USE THEREOF - Provided are fusion proteins of diacylglycerol acyltransferase (DGAT) polypeptides fused to at least one heterologous intracellular localization domain, such as a bacterial membrane-targeting domain, modified photosynthetic microorganism that comprise such fusion proteins, and related methods for improving the production of lipids by photosynthetic microorganisms, including lipids such as triglycerides, fatty acids, and wax esters. | 01-22-2015 |
20150031096 | MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding multizymes (i.e., single polypeptides having at least two independent and separable enzymatic activities) along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these multizymes in plants and oleaginous yeast are disclosed. | 01-29-2015 |
20150031097 | MOBILE PROCESSING SYSTEMS AND METHODS FOR PRODUCING BIODIESEL FUEL FROM WASTE OILS - The present invention improves biodiesel production in several ways. Unique combinations of unit operations and flow configurations are disclosed in mobile processing units that are feedstock-flexible and can be dynamically deployed in a distributed way. In some embodiments, a process includes introducing a waste oil and an alcohol into a reactor with an esterification-transesterification enzymatic catalyst. Free fatty acids are reacted with alcohol to produce fatty acid alkyl esters, and glycerides are reacted with alcohol to produce fatty acid alkyl esters and glycerin. A membrane separator removes glycerin, water, and alcohol. Unreacted free fatty acids are then separated and recycled, to generate a product stream with fatty acid alkyl esters. A genset may be provided for combusting glycerin to produce electrical power and thermal heat as co-products. This biodiesel process may be energy self-sufficient, require no external utilities, and avoid direct discharge of wastewater. | 01-29-2015 |
20150037852 | METHOD AND SYSTEM FOR REDUCING FREE FATTY ACID CONTENT OF A FEEDSTOCK - A method for reducing the free fatty acid content of a feedstock includes the steps of providing a free-fatty-acid-containing feedstock, treating the free-fatty-acid-containing feedstock to reduce the free fatty acid content thereof, where the step of treating includes combining at least one of an algae and a coagulant to the free-fatty-acid-containing feedstock, and producing a product from the treated feedstock. | 02-05-2015 |
20150044736 | FUNGAL CUTINASE FROM MAGNAPORTHE GRISEA - Described are compositions and methods relating to a fungal cutinase cloned from | 02-12-2015 |
20150044737 | PRODUCTION OF DOCOSAHEXAENOIC ACID AND/OR EICOSAPENTAENOIC ACID AND/OR CAROTENOIDS IN MIXOTROPHIC MODE BY NITZSCHIA - New strains of microalgae belonging to the | 02-12-2015 |
20150044738 | PRODUCTION OF EICOSAPENTAENOIC AND/OR ARACHIDONIC ACID IN MIXOTROPHIC MODE BY EUGLENA - New strains of microalgae belonging to the | 02-12-2015 |
20150050707 | TREATMENT OF BIOMASS - A process for the treatment of biomass comprising subjecting biomass to microbial digestion to produce volatile fatty acids and/or solvents followed by wet oxidation to reduce biosolid volume while retaining or increasing the concentration of the volatile fatty acids and/or solvents. | 02-19-2015 |
20150056671 | METHOD FOR PRODUCING FATTY ACID ESTER - Provided is a method of producing a fatty acid ester in a high yield through a simple operation using | 02-26-2015 |
20150056672 | SYSTEMS AND METHODS FOR PRODUCING EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID FROM ALGAE - Provided herein are systems and methods for producing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and/or derivatives and/or mixtures thereof by growing algae that produce the oils containing EPA and/or DHA and/or derivatives and/or mixtures thereof, harvesting the algae with fish in one or more enclosed systems, and then processing fish to separate and purify the EPA and/or DHA. The multi-trophic systems provided herein comprise at least one enclosure that contains the algae and the fishes, and means for controllably feeding the algae to the fishes. Also provided herein are the lipid compositions extracted from the fishes. | 02-26-2015 |
20150064749 | METHODS FOR PRODUCING POLYUNSATURATED FATTY ACID AND LIPID CONTAINING POLYUNSATURATED FATTY ACID - A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA, a microbial cell containing a PUFA, and use of the microbial cell are provided. A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA including culture of a microorganism capable of producing arachidonic acid (ARA) and/or dihomo-gamma-linolenic acid (DGLA) is provided, the method including at least one of the following steps: (a) adding an organic acid in an amount of 0.01 to 5 w/v % to a culture medium after the beginning of main culture; (b) increasing the pH of the culture medium to a range effective for culture after the beginning of the main culture; and (c) adding a metal sulfate in an amount of 0.01 to 0.5 w/w % to the main culture medium. | 03-05-2015 |
20150079645 | MICROBIAL ENGINEERING FOR THE PRODUCTION OF FATTY ACIDS AND FATTY ACID DERIVATIVES - Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production. | 03-19-2015 |
20150104839 | POWDERY LIPASE PREPARATION, METHOD FOR PRODUCING THE SAME AND USE THEREOF - The present invention discloses a powdery lipase preparation which is a granulated material comprising a lipase derived from | 04-16-2015 |
20150111263 | METHODS AND PRODUCTS FOR GENERATING OILS - Compositions and methods for enhancing secretion of oils from oil producing cells are described herein. In particular, secretion of oils is enhanced by treating the cells with herbicides. The oils generated according to the invention may be useful, for example, in the production of biofuels. | 04-23-2015 |
20150111264 | Method for Producing Lipid - Provided is a method for producing a lipid, by which lipid including a high ratio of medium-chain fatty acids can be produced efficiently. | 04-23-2015 |
20150111265 | MICROBIAL CONVERSION OF METHANE - This invention relates to a process for producing lipids and amino acids from a gaseous substrate comprising methane and oxygen. The process uses a culture of a methanotrophic microorganism in a liquid nutrient medium. The methanotrophic microorganism can be a | 04-23-2015 |
20150118723 | Apparatus and method for generating eco-conscious products from waste - An assembly for generating eco-conscious products from waste uses an anaerobic digester for anaerobically processing waste matter to produce anaerobic products selected from the group of products consisting of biogas, liquid digestate, solid digestate, and mixtures thereof One or more eco-assemblies receive the anaerobic products to generate at least one eco-conscious product. The eco-assemblies include one or more of an eco-matrix molder, an algae cultivator, a fast-pyrolysis chamber, a soil enhancer, a RDF pelletizer, and a hydroponic apparatus. A method is provided for generating eco-conscious products from waste by anaerobically processing waste matter to produce one or more of the anaerobic products, which are conveyed to one or more eco-assemblies to generate at least one eco-conscious product. | 04-30-2015 |
20150125911 | METHOD FOR PRODUCING OXO FATTY ACID AND RARE FATTY ACID - The present invention provides a production method of oxo fatty acid, as well as rare fatty acids such as conjugated fatty acid, hydroxylated fatty acid, partially saturated fatty acid and the like, which uses 4 kinds of enzymes (fatty acid-hydratase, hydroxylated fatty acid-dehydrogenase, oxo fatty acid-isomerase, oxo fatty acid-enone reductase) derived from | 05-07-2015 |
20150125912 | MULTI-STAGE SYNTHESIS METHOD WITH SYNTHESIS GAS - The invention provides a process for the preparation of hydrocarbons substituted with at least one group containing at least one oxygen atom, comprising the process steps
| 05-07-2015 |
20150125913 | INTEGRATED ETHANOL AND BIODIESEL FACILITY - An integrated facility for the co-production of ethanol and biodiesel fuel is provided. Ethanol and corn oil, the primary product and a by-product from the ethanol plant, are utilized as feedstocks for a biodiesel plant operating within the same general facility as the corn ethanol plant. By-products of the biodiesel plant, principally crude liquid glycerol and gaseous ethanol or methanol, are recycled to various parts of the ethanol plant. | 05-07-2015 |
20150125914 | TAILORED OILS - Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type. | 05-07-2015 |
20150132812 | TWO-STAGE PROCESS FOR PRODUCING OIL FROM MICROALGAE - A process for production of biofuels from algae can include cultivating an oil-producing algae by promoting sequential photoautotrophic and heterotrophic growth. The method can further include producing oil by heterotrophic growth of algae wherein the heterotrophic algae growth is achieved by introducing a sugar feed to the oil-producing algae. An algal oil can be extracted from the oil-producing algae, and can be converted to form biodiesel. | 05-14-2015 |
20150140618 | LIPASE IN SHORT-CHAIN ESTERIFICATION OF FATTY ACIDS - Patatin, a lipase obtainable from potatoes, has been found to have advantages in the esterification reaction of fatty acids to yield fatty acid alkyl esters. Methods for application of this lipase activity are described, as well as the use of patatin in various industrial processes. | 05-21-2015 |
20150140619 | USE OF MARINE ALGAE FOR CO-PRODUCING ALKENONES, ALKENONE DERIVATIVES, AND CO-PRODUCTS - A method comprising a series of selective extraction techniques for the parallel production of biodiesel and isolation of several valuable co-products including an alkenone hydrocarbon mixture of the kerosene/jet fuel range (primarily C10-, C12-, and C17-hydrocarbons) and fucoxanthin, a high-valued carotenoid, from the marine alkenone-producing microalgae | 05-21-2015 |
20150147790 | ACYL-ACP THIOESTERASE GENES AND USES THEREFOR - The present invention provides novel genes encoding Class II acyl-ACP thioesterases and variants thereof that are active on C8, C10, C12, C14, C16, and C18 acyl-ACP substrates. The thioesterases can be introduced into transgenic organisms, including microorganisms and photosynthetic organisms, for producing fatty acids and fatty acid products. | 05-28-2015 |
20150291892 | LOW TEMPERATURE, LOW PRESSURE UPGRADING AND STABILIZATION OF BIO-OIL OR BIO-OIL FRACTIONS - The present application discloses low temperature, low pressure methods (LTLP) for upgrading and/or stabilizing bio-oil or a bio-oil fraction. One method comprises providing a bio-oil or bio-oil fraction and hydrogen, which are reacted in the presence of a catalyst at a temperature of less than 150° C. and a pressure of less than 100 bar (absolute) to produce a hydrogenated liquid oil at a carbon yield of over 75%. Another method comprises providing a bio-oil or bio-oil fraction, providing oxygen reducing reaction conditions, and reacting the bio-oil or bio-oil fraction under the oxygen reducing reaction conditions at LTLP to produce an upgraded bio-oil product containing fewer carbonyls than the bio-oil or bio-oil fraction. Yet another method comprises providing a bio-oil or bio-oil fraction and a solution comprising one or more fermentation organisms and a sugar source. The solution and bio-oil or bio-oil fraction are combined to obtain a fermentation mixture, which is incubated at 15° C. to 30° C. for 16 to 72 hours to produce an upgraded bio-oil fermentation product containing fewer carbonyls than the bio-oil or bio-oil fraction. | 10-15-2015 |
20150299679 | ENZYME VARIANTS WITH IMPROVED ESTER SYNTHASE PROPERTIES - The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells. | 10-22-2015 |
20150299745 | COMPOSITIONS AND METHODS FOR BIOLOGICAL PRODUCTION OF FATTY ACID DERIVATIVES - The present disclosure provides compositions and methods for biologically producing fatty acid derivatives, such as fatty alcohols, from recombinant C | 10-22-2015 |
20150299746 | PROCESS FOR THE PRODUCTION OF LIPIDS FROM BIOMASS - Process for the production of lipids from biomass including at least one polysaccharide, which comprises fermentation of a biomass hydrolysate using an oleaginous microorganism and separation of oleaginous cellular biomass comprising lipids. An aqueous fraction obtained by reverse osmosis of a spent fermentation broth is recycled as a feed for growth of the oleaginous microorganism. The lipids thus obtained can be advantageously used in the production of biodiesel or green diesel which can be used as such, or in a mixture with other automotive fuels. | 10-22-2015 |
20150299747 | METHOD FOR PRODUCING TRANSESTERIFIED FAT AND/OR OIL - An object of the present invention is to provide a method in which a fat and/or oil is produced by a transesterification reaction using a lipase. Specifically, the present invention relates to a method for producing a transesterified fat and/or oil, comprising: (1) a low-temperature clay treatment step of bringing a fat and/or oil and a clay into contact with each other at 30 to 80° C. to obtain a reaction substrate; and (2) a step of subjecting the reaction substrate to a transesterification reaction in the presence of a lipase-containing composition. | 10-22-2015 |
20150307902 | RECYCLING OF ENZYMES FROM BIOREACTORS - A method of conducting an enzymatic process in which the enzymes are recovered and reused in at least a second iteration of the process, wherein each iteration of the process comprises the steps of:
| 10-29-2015 |
20150315617 | METHODS AND COMPOSITIONS FOR PRODUCING FATTY ALCOHOLS OR FATTY ALDEHYDES - Compositions and methods for producing hydrocarbons such as aldehydes, alkanes, and alkenes are described herein. Certain hydrocarbons can be used in biofuels. | 11-05-2015 |
20150315618 | Enzymatic Chemical Processing in a Fiber Conduit Apparatus - A method is provided which introduces a first stream proximate a plurality of fibers positioned within a fiber conduit apparatus such that the first stream constitutes a phase substantially constrained to the surface of the fibers. The method further introduces an enzyme into the apparatus and introduces a second stream into the apparatus that is in contact with and is substantially immiscible with the first stream. The first stream, the enzyme and the second stream are introduced into the apparatus such that the enzyme interacts with a species from one of the first and second streams and compositions of the first and second streams are altered. An apparatus is provided which includes a plurality of fibers positioned longitudinally within a conduit between two fluid inlets and a fluid outlet, wherein the plurality of fibers have one or more enzymes immobilized thereon. | 11-05-2015 |
20150322417 | Oleaginous Bacterial Cells and Methods for Producing Lipids - This invention relates to cells and methods for producing lipids using cellulosic carbon source. More specifically the invention relates to oleaginous bacterial cells, wherein genes encoding at least one cellulolytic activity has been introduced. This invention also relates to methods for lipid production by cultivating an oleaginous bacterial strain or strains capable of expressing one or more cellulolytic activity. | 11-12-2015 |
20150322442 | Novel Shuttle Vector Capable of Transforming Multiple Genera of Cyanobacteria - A plasmid vector for the production of compounds in cyanobacteria is described which is capable of being efficiently transformed to and replicating in a broad range of cyanobacterial species. | 11-12-2015 |
20150322466 | MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS - A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from | 11-12-2015 |
20150322467 | METHOD FOR OPTIMIZING PRODUCTION OF EICOSAPENTAENOIC ACID (EPA) IN A RECOMBINANT HOST - The present invention relates to a method for optimizing production of eicosapentaenoic acid (EPA) production by cloning genes into a bacterial host, most preferably a modified | 11-12-2015 |
20150322468 | METHOD OF PRODUCING BIOFUEL USING MICROALGAE CULTURES - The present invention relates to methods of improving TAG production of microalgae, for example, the microalgae in microalgal mass culture systems, and compositions of the improved microalgae. The method of improving TAG production may be increasing the yield of TAG produced by each microalgae or increasing the total yield of a mass culture system by increasing the number of microalgae in the mass culture system. | 11-12-2015 |
20150337345 | METHODS OF OIL PRODUCTION IN MICROORGANISMS - Provided herein are methods for producing one or more polyunsaturated fatty acids. The methods include the steps of providing a microorganism capable of producing polyunsaturated fatty acids, providing a medium comprising a high concentration of one or more carbon sources, low pH, or both, and culturing the microorganism in the medium under sufficient conditions to produce the one or more polyunsaturated fatty acids. Also provided are methods of culturing one or more microorganisms. The methods include culturing the microorganisms in a medium comprising a first amount of one or more carbon sources at a first concentration level, monitoring a carbon source concentration until the carbon source concentration is reduced below the first concentration level, and adding to the medium a second amount of one or more carbon sources to increase the carbon source concentration to a second concentration level. | 11-26-2015 |
20150344917 | Microbial Oils with Lowered Pour Points, Dielectric Fluids Produced Therefrom, and Related Methods - Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids. | 12-03-2015 |
20150344918 | COMPOSITIONS AND METHODS FOR MAKING AND MODIFYING OILS - The invention provides novel methods for making or modifying oils, e.g., plant animal or microbial oils, such as vegetable oils or related compounds, that are low in a particular fatty acid(s), for example, low linoleic oils, linolenic oils, low palmitic oils, low stearic oils or oils low in a combination thereof. | 12-03-2015 |
20150353968 | Production of Fatty Acid Alkyl Esters - A method for producing fatty acid alkyl esters, wherein a solution comprising triglyceride, alcohol, water, and glycerol is contacted with a lipolytic enzyme. | 12-10-2015 |
20150353969 | METHOD FOR IMPROVING YIELD OF ENZYMATIC PREPARATION OF BIODIESEL FROM GREASES - Provided in the present invention is a method for improving the yield of enzymatic preparation of biodiesel from greases, comprising: during enzymatic reaction of short-chain alcohols with greases to prepare biodiesel, introducing online dehydration steps, which lead the volatile gases in the enzyme reactor to enter into the low-temperature absorption tank, and the dehydrated gases flow back into the enzyme reactor. The formation of gas-circulation can constantly bring the moisture inside the enzyme reactor out. | 12-10-2015 |
20150353970 | ENZYMATIC TRANSESTERIFICATION/ESTERIFICATION PROCESSING SYSTEMS AND PROCESSES EMPLOYING LIPASES IMMOBILZED ON HYDROPHOBIC RESINS - Disclosed are processing systems and processes for carrying out enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food, cosmetics, pharmaceuticals and detergents industries. | 12-10-2015 |
20150353972 | MICROORGANISM PRODUCING DOCOSAHEXAENOIC ACID AND UTILIZATION THEREOF - According to the present invention, a microorganism belonging to the genus | 12-10-2015 |
20150360984 | PROCESSING AND APPLICATION OF A PURIFICATION SYSTEM FOR GOLD MINING, EXTRACTION OF MINERALS AND GROWTH OF ALGAE BIOMASS - The present disclosure describes a process for using saline water, and saline reject water produced in water purification, to use for gold mining production, and growing and harvesting algae. The disclosure also describes a method for growing and harvesting algae utilizing saline water as growth medium for recycling waste water to extract the remaining metals out of waste water. The harvested algae may be used in various applications including but not limited to water purification for gold mining production and to extract metals out of remaining waste water. | 12-17-2015 |
20150361461 | METHOD FOR PRODUCING DHA THROUGH SOLID CULTURE AND LIQUID FERMENTATION OF SCHIZOCHYTRIUM - The invention relates to a method for fermentation production of DHA, in particular a method for producing DHA through solid culture and liquid fermentation of | 12-17-2015 |
20160002566 | PROCESSES FOR PRODUCING INDUSTRIAL PRODUCTS FROM PLANT LIPIDS - The present invention relates to methods of producing industrial products from plant lipids, particularly from vegetative parts of plants. In particular, the present invention provides oil products such as biodiesel and synthetic diesel and processes for producing these, as well as plants having an increased level of one or more non-polar lipids such as triacylglycerols and an increased total non-polar lipid content. In one particular embodiment, the present invention relates to combinations of modifications in two or more of lipid handling enzymes, oil body proteins, decreased lipid catabolic enzymes and/or transcription factors regulating lipid biosynthesis to increase the level of one or more non-polar lipids and/or the total non-polar lipid content and/or mono-unsaturated fatty acid content in plants or any part thereof. In an embodiment, the present invention relates to a process for extracting lipids. In another embodiment, the lipid is converted to one or more hydrocarbon products in harvested plant vegetative parts to produce alkyl esters of the fatty acids which are suitable for use as a renewable biodiesel fuel. | 01-07-2016 |
20160002580 | PHOTOBIOREACTOR APPARATUS, METHOD AND APPLICATION - A photobioreactor includes an input light delivery component and a plurality of photobioreactor media disposed in a stacked relationship, each of which is coupled to the input light delivery component, wherein each photobioreactor medium is adapted to be inoculated with a photosynthetic, biofuel-producing culture, further wherein each photobioreactor medium includes at least one hollow fiber membrane (HFM) disposed thereon in a region that is adapted to be inoculated with the photosynthetic, biofuel-producing culture, that can transport at least one of a gas and a photobioreactor product producible by the photosynthetic, biofuel-producing culture, wherein each photobioreactor medium is characterized by an input light-scattering mechanism. A method for making a biofuel including providing a stacked photobioreactor assembly including a plurality of unit photobioreactor mediums each of which is inoculated with a photosynthetic, biofuel-producing culture, characterized by a light scattering mechanism and including at least one hollow fiber membrane (HFM) disposed in-situ on the photobioreactor medium; providing a nutrient gas to the biofuel-producing culture via the at least one HFM; and illuminating each unit photobioreactor medium with a selected spectrum of input light via the light scattering mechanism in each unit photobioreactor medium. | 01-07-2016 |
20160002680 | ACIDOPHILIC FUSARIUM OXYSPORUM STRAINS, METHODS OF THEIR PRODUCTION AND METHODS OF THEIR USE - The present invention provides isolated acidophilic | 01-07-2016 |
20160002681 | ACP-MEDIATED PRODUCTION OF FATTY ACID DERIVATIVES - The disclosure relates to recombinant microorganisms that exhibit an increased expression of an acyl carrier protein (ACP) resulting in production of fatty acid derivatives. The disclosure further relates to methods of using the recombinant microorganisms in fermentation cultures in order to produce fatty acid derivatives and related compositions. | 01-07-2016 |
20160010066 | Novel Ketoacyl ACP Synthase Genes and Uses Thereof | 01-14-2016 |
20160017352 | ALGAL STRAIN WITH REDUCED BETA GLUCAN SYNTHASE ACTIVITY - The present invention provides compositions of a modified algal cell and methods of making thereof. In particular, the modified cell has suppressed expression or activity of endogenous beta glucan synthase 1 (BGS1) and increased lipid synthesis when grown under nutrient deficient conditions. | 01-21-2016 |
20160017386 | ALTERING THE INTERFACE OF HYDROCARBON-COATED SURFACES - Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or to benefit pipeline maintenance. | 01-21-2016 |
20160017387 | GENETICALLY MODIFIED MICROORGANISM FOR PRODUCING LONG-CHAIN DICARBOXYLIC ACID AND METHOD OF USING THEREOF - Described herein are genetically-modified microorganisms for producing long-chain dicarboxylic acids and methods of using the microorganisms. The microorganisms contain a first nucleic acid encoding an | 01-21-2016 |
20160017388 | PRODUCTION OF FATTY ACIDS BY HETEROLOGOUS EXPRESSION OF GENE CLUSTERS FROM MYXOBACTERIA - The invention relates to a process for producing one or more polyunsaturated fatty acids by means of heterologous gene expression comprising the steps of providing a production organism which comprises a heterologous gene cluster encoding a polyunsaturated fatty acid biosynthetic pathway encompassing a subsequence ER encoding an enoylreductase and a subsequence AT encoding an acyltransferase,
| 01-21-2016 |
20160024359 | SYSTEM AND METHOD FOR TREATMENT OF BIOMASS PRODUCTS OR RESIDUES AND RESULTING COMPOSITION - A system and method for treatment of biomass products or residues to obtain valuable adhesives and composite materials is described herein. Some embodiments do not require purification of a biomass product or residue to produce an adhesive. Some embodiments comprise a treatment of post extraction algae residue configured to produce an adhesive. Use of post extraction algae residue adds value to alternative energy produced by extracting oil from algae. | 01-28-2016 |
20160024535 | ELECTROCHEMICAL DETOXIFICATION METHOD OF WOOD-BASED HYDROLYSATE FOR PRODUCING BIOCHEMICALS OR BIOFUELS, AND DETOXIFIED WOOD-BASED HYDROLYSATE - Provided is a method for producing a saccharified solution of wood-based biomass having toxicity reduced or removed, comprising the steps of: preparing a saccharified solution in which wood-based biomass has been pretreated by hydrolysis; and reducing toxicity in the saccharified solution using an electrochemical detoxification method. The method is capable of efficiently removing the toxicity of compounds which are produced during the pretreatment process and inhibit growth of microorganisms and fermentation. In addition, the method is capable of increasing the efficiency of production by minimizing sugar loss and additional costs during the toxicity removal process. | 01-28-2016 |
20160032216 | ANTIMICROBIAL COMPOSITION CONTAINING LAURIC ACID AND METHODS FOR THEIR PRODUCTION - The present invention relates to the method of producing an antimicrobial composition characterized in that it comprises the steps of: a) splitting an Lurie oil treated with activated carbon; and b) recovering a composition comprising 90% or more, by weight of free fatty acids. The invention further relates to antimicrobial compositions obtained by said process, to feed, food or beverage comprising said composition and to their uses. | 02-04-2016 |
20160032331 | Methods and Systems For Biological Coal-to-Biofuels and Bioproducts - Methods and systems for the biological conversion of pretreated or solubilized coal or waste coal into biofuels. Coal ( | 02-04-2016 |
20160032332 | VARIANT THIOESTERASES AND METHODS OF USE - The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein. | 02-04-2016 |
20160040171 | METHOD TO PRODUCE HYDROCARBON FROM C-1 SUBSTRATE - The disclosure relates to biological methods of making a hydrocarbon feedstock wherein one-carbon substrates are converted into useful chemicals and fuels. Particularly, genetically engineered bacteria are used to make C4-C10 fatty acids or derivatives from one-carbon substrates such as methanol and carbon dioxide. | 02-11-2016 |
20160040175 | ENGINEERING MICROORGANISMS - Provided are Thraustochytrid and | 02-11-2016 |
20160040198 | BIOREFINERY SYSTEM, METHODS AND COMPOSITIONS THEREOF - The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C | 02-11-2016 |
20160046968 | Novel strain secreting fatty acids by phospholipase and method for producing fatty acids using it - A bacterial strain secreting fatty acids, the strain inducing fatty acids to be extracellularly secreted by using phospholipase expressed in the periplasmic space of cell. When a method of producing fatty acids by using the bacterial strain secreting fatty acids is used, fatty acids extracellularly secreted are continuously obtained without apoptosis, leading to lower costs and higher production efficiency. Phospholipase, unlike thioesterase, which is a typical fatty-acid degrading enzyme, decomposes phospholipid to produce free fatty acids. Accordingly, by using the substrate specificity of two different phospholipases, a fatty acid having a specific composition can be selectively produced. Unlike in a typical method in which fat is obtained from cells or tissues, fatty acids secreted during cell growth are obtainable by biding to a hydrophobic material without an extraction process using an organic solvent in large quantities. Accordingly, a more economical, environmentally friendly bio-oil production process can be realized. | 02-18-2016 |
20160060663 | PRODUCTION OF FATTY ACIDS ESTERS - A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester. | 03-03-2016 |
20160083757 | ENZYMATIC PROCESS FOR THE PRODUCTION OF MANNOSYLERYTHRITOL LIPIDS FROM LIGNOCELLULOSIC MATERIALS - The present invention relates to processes for the production of microbial glycolipids, mannosylerythritol lipids (MEL), from lignocellulosic carbon source. These processes are characterized in that the use of lignocellulosic materials for the production of a microbial glycolipids, MEL, comprising a fermentation preferably using fungi of the genus | 03-24-2016 |
20160083758 | ACYL-ACP THIOESTERASES AND MUTANTS THEREOF - Novel plant acyl-ACP thioesterase genes of the FatB and FatA classes and proteins encoded by these genes are disclosed. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Expression of the novel and/or mutated FATB and FATA genes is demonstrated in oleaginous microalga host cells. Furthermore, a method for producing an oil elevated in one or more of C12:0, C14:0, C16:0, C18:0 and/or C18:1 fatty acids includes transforming a cell with novel and/or mutated FATB and/or FATA genes, e.g., having an N-terminal deletion. The cells produce triglycerides with altered and useful fatty acid profiles. | 03-24-2016 |
20160090612 | GENE DISTRUPTANTS PRODUCING FATTY ACYL-COA DERIVATIVES - This invention provides microbial organisms, particularly yeasts such as | 03-31-2016 |
20160097029 | ENGINEERED CYANOBACTERIA WITH ENHANCED SALT TOLERANCE - The present invention relates to a recombinant cyanobacterium with enhanced halotolerance and compositions thereof, methods of producing the recombinant cyanobacterium, and methods of using the same for biofuel production. The invention also relates to transformed | 04-07-2016 |
20160097065 | METHODS OF MAKING SURFACTANT AND CLEANING COMPOSITIONS THROUGH MICROBIALLY PRODUCED BRANCHED FATTY ALCOHOLS - The invention provides a surfactant and/or a cleaning composition comprising a microbially produced branched fatty alcohol or a derivative thereof. The invention also provides a household cleaning composition and a personal or pet care cleaning composition comprising a microbially produced branched fatty alcohol or a derivative thereof. | 04-07-2016 |
20160097066 | Mutant Yeasts Capable of Producing An Unusual Fatty Acid - The present invention relates to a method for obtaining a mutant strain of oleaginous yeast which is useful as a template strain of yeast for obtaining other mutant strains of oleaginous yeast which are capable of producing an unusual fatty acid. The present invention also relates to the mutant strains of yeast obtained by said method. | 04-07-2016 |
20160102287 | Method and Apparatus for Growing Microbial Cultures that Require Gaseous Electron Donors, Electron Acceptors, Carbon Sources, or Other Nutrients - Compositions and methods and apparatus for growth and maintenance of microorganisms and/or bioprocesses using one or more gases as electron donors, electron acceptors, carbon sources, or other nutrients, and for a bioprocess that converts hydrogen and carbon dioxide, or syngas, or producer gas into lipid products, bio-based oils, or other biochemical products. | 04-14-2016 |
20160102327 | METHODS AND COMPOSITIONS FOR PRODUCING FATTY ALCOHOLS - Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described. | 04-14-2016 |
20160102328 | Microalgae Aurantiochytrium sp. LA3 (KCTC12685BP) and Method for Preparing Bio-Oil Using the Same - Provided herein are microalgae of a Thraustochytrid and a method for preparing bio-oil using the same, and more particularly, | 04-14-2016 |
20160122741 | Molecules Associated with Fatty Acid Biosynthetic Pathways and Uses Thereof - The present disclosure relates in part to recombinant microorganisms that include non-native genes encoding PUFA-PKS polypeptides, and to methods of making and using such microorganisms for producing at least one PUFA. In particular, the disclosure further relates to methods and related materials useful for the production of at least one PUFA by heterologous expression of the nucleic acid sequences disclosed herein encoding PUFA-PKS polypeptides. | 05-05-2016 |
20160122786 | COMPOSITIONS AND METHODS FOR GENERATION OF BIOFUELS - Provided herein are compositions and method for generation of biofuels. In particular, provided herein are modified bacteria for use in processing intermediates of algae biomass processing. | 05-05-2016 |
20160122787 | FERMENTATION PROCESS FOR THE PRODUCTION OF LIPIDS - The invention provides methods and systems for the production of lipid products from a gaseous substrate using a two stage fermentation process. The method comprises providing a gaseous substrate comprising CO, CO | 05-05-2016 |
20160130599 | MODIFIED DIATOMS FOR BIOFUEL PRODUCTION - The invention provides engineered diatoms and methods of producing oil using diatoms. The invention also provides methods of modifying the lipids quantity and/or quality produced by diatom organisms through genome engineering. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured from such modified diatoms. | 05-12-2016 |
20160130615 | Acyl-ACP Thioesterase - An acyl-ACP thioesterase consisting of an amino acid sequence of the 72 | 05-12-2016 |
20160138061 | FATTY ACID AND DERIVATIVES PRODUCTION - At least one fatty acid and/or derivative thereof is produced from a gas containing H | 05-19-2016 |
20160145599 | Mutant Yeasts Having an Increased Production of Lipids and of Citric Acid - The present invention relates to a mutant yeast strain, in which at least the expression or the activity of the 2-methyl-citrate dehydratase is inhibited, and to the use of said strain for the production of lipids and of citric acid. | 05-26-2016 |
20160145657 | Methods and Materials for Producing 7-Carbon Chemicals via a C9 Route - This document describes biochemical pathways for producing 7-hydroxyheptanoic acid using a polypeptide having monooxygenase activity to form a 8-hydroxynonanoate intermediate, which can be converted to 7-hydroxyheptanoate using a polypeptide having monooxygenase activity, a polypeptide having secondary alcohol dehydrogenase activity, and a polypeptide having esterase activity. 7-hydroxyheptanoic acid can be enzymatically converted to pimelic acid, 7-aminoheptanoic acid, heptamethylenediamine or 1,7 heptanediol. This document also describes recombinant hosts producing 7-hydroxyheptanoic acid as well as pimelic acid, 7-aminoheptanoic acid, heptamethylenediamine and 1,7 heptanediol. | 05-26-2016 |
20160145658 | PROCESS AND COMPOSITION FOR PRODUCTION OF ORGANIC PRODUCTS - The present invention comprises a process to produce organic products from a single-carbon substrate; microbial compositions used in the process; and a process to isolate microorganisms for the process. | 05-26-2016 |
20160160240 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to proceed ethanol and/or butanol, e.g., by fermentation. | 06-09-2016 |
20160160246 | Oleaginous Microorganism Disruption Process Using Supersonic Disperser and Method for Producing Bio-Oil Using Same - Provided are an oleaginous microorganism disruption process using a supersonic disperser and a method for producing bio-oil using the same. The method for producing bio-oil according to the present invention induces a cell disruption of oleaginous microorganisms without a separate drying process, thereby providing a method for continuously producing bio-oil in an economical and simple manner. In addition, the method of the present invention induces a cell disruption of oleaginous microorganisms without a heating process, thereby producing bio-oil without a change in physical properties due to the heat. | 06-09-2016 |
20160160247 | Modified algae strain and method of triacylglycerol accumulation using said strain - The present invention relates to a genetically engineered algae strain in which the expression of the CGI-58 gene or homologous gene thereof is silenced. The present invention further relates to a method of triacylglycerol accumulation using said genetically engineered diatom and/or diatom strain. | 06-09-2016 |
20160167996 | MICROBIAL CONSORTIUM FOR WASTEWATER TREATMENT | 06-16-2016 |
20160168603 | MATERIALS AND METHODS FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS IN RECOMBINANT BACTERIA | 06-16-2016 |
20160168604 | COMPOSITIONS AND METHODS FOR MAKING AND MODIFYING OILS | 06-16-2016 |
20160177349 | Processes and Systems for Catalytic Manufacture of Wax Ester Derivatives | 06-23-2016 |
20160177350 | Processes and Systems for Catalytic Manufacture of Wax Ester Derivatives | 06-23-2016 |
20160186109 | REACTOR FOR BULK PRODUCTION OF PHOTOSYNTHETIC MICROORGANISMS - An algae production reactor system according to the invention comprises a reactor vessel which is provided with:—one or more liquid inlets and one or more liquid outlets;—one or more gas inlets at the bottom, said gas inlets being connected with a source of carbon dioxide, and one or more gas outlets at the top of the vessel;—vertically interspaced and joined pairs of double glass plates which are at least partially submerged in the reactor liquid, said double glass plates having a layer of light-scattering particles in between and having a flat side being exposed to a light source; and—means for vertically circulating reactor liquid. | 06-30-2016 |
20160186163 | ENGINEERED MICROBES AND METHODS FOR MICROBIAL OIL OVERPRODUCTION FROM CELLULOSIC MATERIALS - The invention relates to engineering microbial cells for utilization of cellulosic materials as a carbon source, including xylose. | 06-30-2016 |
20160186191 | Tailored Oils Produced from Recombinant Heterotrophic Microorganisms - Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants. | 06-30-2016 |
20160194599 | NOVEL METHOD FOR ADHERENT CULTURE IN REGION FORMED BETWEEN WATER-ABSORBENT POLYMER GEL AND SUBSTRATE, METHOD FOR MANUFACTURING BIOMASS, AND NOVEL MICROALGA | 07-07-2016 |
20160194600 | METHOD FOR LIQUID-SURFACE FLOATING CULTURE OF MICROALGAE USING MICROALGAE ON BOTTOM SURFACE AS SEED ALGAE, METHOD FOR PRODUCING ALGAL BIOMASS, AND MICROALGA | 07-07-2016 |
20160194671 | CULTURE METHOD FOR MICROALGAE THAT IMPROVES OIL CONTENT RATIO, METHOD FOR MANUFACTURING ALGAL BIOMASS, AND NOVEL MICROALGA | 07-07-2016 |
20160194672 | Tailored Oils | 07-07-2016 |
20160201007 | PROCESS OF PRODUCTION OF OIL FROM MICROALGAE | 07-14-2016 |
20160201075 | REGULATOR OF ALGAL LIPID METABOLISM AND CELLULAR QUIESCENCE AND ITS APPLICATIONS | 07-14-2016 |
20160201096 | Fatty Acid Production in Cell-Free Systems | 07-14-2016 |
20160201098 | METHOD FOR GENERATING OIL/FAT COMPONENT, METHOD FOR PRODUCING HIGHER UNSATURATED FATTY ACID, AND CHLAMYDOMONAS SP. JSC4 | 07-14-2016 |
20160376566 | Peroxygenase Variants - The present invention relates to peroxygenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 12-29-2016 |
20160376617 | MICROBIAL OILS WITH LOWERED POUR POINTS, DIELECTRIC FLUIDS PRODUCED THEREFROM, AND RELATED METHODS - Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids. | 12-29-2016 |
20170233689 | METHODS AND MATERIALS FOR CULTIVATION AND/OR PROPAGATION OF A PHOTOSYNTHETIC ORGANISM | 08-17-2017 |
20170233775 | TWO-STAGE PROCESS FOR PRODUCING OIL FROM MICROALGAE | 08-17-2017 |
20170233776 | METHOD FOR PREPARING DIGLYCERIDE USING BUBBLE COLUMN REACTOR | 08-17-2017 |
20190144806 | METHODS AND MATERIALS FOR CULTIVATION AND/OR PROPAGATION OF A PHOTOSYNTHETIC ORGANISM | 05-16-2019 |
20190144897 | HIGH LEVEL PRODUCTION OF LONG-CHAIN DICARBOXYLIC ACIDS WITH MICROBES | 05-16-2019 |
20190144898 | IMPROVED MICROBIAL PRODUCTION OF FATS | 05-16-2019 |
20220136018 | SEMI-BIOSYNTHETIC PRODUCTION OF FATTY ALCOHOLS AND FATTY ALDEHYDES - The present application relates to methods of producing one or more fatty alcohols and/or one or more fatty aldehydes from one or more unsaturated lipid moieties by combining the obtainment or production of the one or more unsaturated lipid moieties from a biological source with conversion by non-biological means of the one or more unsaturated lipid moieties to one or more fatty alcohols and/or one or more fatty aldehydes. The present application also relates to recombinant microorganisms having a biosynthesis pathway for the production of one or more unsaturated lipid moieties. The one or more fatty alcohols can further be chemically converted to one or more corresponding fatty acetates. The one or more fatty alcohols, one or more fatty aldehydes and/or one or more fatty acetates produced by the methods described herein may be one or more insect pheromones, one or more fragrances, one or more flavoring agents, or one or more polymer intermediates. | 05-05-2022 |