Entries |
Document | Title | Date |
20080254518 | Liquefaction Processes - The present invention relates to method of liquefying starch-containing material, wherein the method is carried out in stages. The invention also relates to a process of producing ethanol comprising a liquefaction step carried out according to the liquefaction method of the invention. | 10-16-2008 |
20080299622 | Starch Hydrolysis Using Phytase with an Alpha Amylase - The present invention relates to a method for liquefying starch comprising contacting a slurry of a starch substrate with an enzyme composition comprising a phytase and an alpha amylase. The invention also relates to an enzyme mixture comprising a phytase derived from | 12-04-2008 |
20090011473 | Saccharifying cellulose - Dissolution, partial dissolution or softening of cellulose in an ionic liquid (IL) and its subsequent contact with anti-solvent produces regenerated cellulose more amorphous in structure than native cellulose, which can be separated from the IL/anti-solvent mixture by mechanical means such as simple filtration or centrifugation. This altered morphology of IL-treated cellulose allows a greater number of sites for enzyme adsorption with a subsequent enhancement of its saccharification. The IL-treated cellulose exhibits significantly improved hydrolysis kinetics with optically transparent solutions formed after about two hours of reaction. This provides an opportunity for separation of products from the catalyst (enzyme) easing enzyme recovery. With an appropriate selection of enzymes, initial hydrolysis rates for IL-treated cellulose were up to two orders of magnitude greater than those of untreated cellulose. Due to the non-volatility of the IL, anti-solvent can be easily stripped from the IL/anti-solvent mixture for recovery and recycle of both the ionic liquid and anti-solvent. | 01-08-2009 |
20090011474 | Process for producing sugars from cellulosic biomass - A process for increasing production of sugars from cellulose in a plant biomass using ammonia after swelling of the biomass with water and enzymatic hydrolysis is described. The sugars are preferably fermented to an alcohol, particularly ethanol as a fuel for vehicles. | 01-08-2009 |
20090035826 | METHOD FOR THE PRODUCTION OF ALCOHOL FROM A PRETREATED LIGNOCELLULOSIC FEEDSTOCK - A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose. | 02-05-2009 |
20090047715 | PROCESS FOR STARCH LIQUEFACTION AND FERMENTATION - The presently disclosed subject matter provides improved processes for processing starch from plant sources, including processes for starch liquefaction, for simultaneous liquefaction and saccharification, and for the preparation of ethanol. These processes can be performed without a pH adjustment and at relatively low temperatures. The processes can involve the use of starch-containing plant material derived from plants that express starch-digesting enzymes. The presently disclosed subject matter further relates to improved processes for the preparation of other starch-derived products, including dried distiller grain (dried distiller grain) and dried distiller grain and solubles (dried distiller grain and solubles), and to the starch-derived products, themselves. | 02-19-2009 |
20090093027 | Process for producing sugars and ethanol using corn stillage - A process for producing sugars from lignocellulosic materials such as corn stover by the addition of corn stillage as a carbon source is disclosed. The sugars are formed by treating the combination of the corn stillage and the lignocellulosic materials with hydrolytic enzymes. The sugars can be fermented to ethanol, and the process improves ethanol production economies with an increased sugar yield from the lignocellulosic materials. | 04-09-2009 |
20090098616 | ENZYMATIC TREATMENT OF LIGNOCELLULOSIC MATERIALS - A method for treating plant materials to release fermentable sugars is disclosed. More specifically, a two-stage enzymatic hydrolysis process for treating lignocellulosic materials and producing a sugar rich process stream that may subsequently be subjected to fermentation to produce biofuels and chemicals is disclosed. | 04-16-2009 |
20090098617 | ENZYMATIC TREATMENT UNDER VACUUM OF LIGNOCELLULOSIC MATERIALS - A method for treating plant materials to release fermentable sugars is disclosed. More specifically, an enzymatic hydrolysis process for treating lignocellulosic materials performed under vacuum and producing a sugar rich process stream that may subsequently be subjected to fermentation to produce biofuels and chemicals is disclosed. | 04-16-2009 |
20090098618 | TREATMENT OF LIGNOCELLULOSIC MATERIALS UTILIZING DISC REFINING AND ENZYMATIC HYDROLYSIS - A method for treating plant materials to release fermentable sugars is disclosed. More specifically, lignocellulosic materials are subjected to disc refining together with enzymatic hydrolysis to produce a sugar rich process stream that may subsequently be subjected to fermentation to produce biofuels and chemicals is disclosed. | 04-16-2009 |
20090148913 | Storage of Cellulosic Feedstocks to Facilitate Biofuel Production - A method for storing cellulosic feedstock materials, particularly corn cobs, to facilitate the production of ethanol therefrom is effective to store feedstocks having a moisture content between about 20% and 50%. The method is initiated with the piling of the feedstock into a smooth, substantially rounded pile. The cellulosic biomass desirably has a substantially uniform average moisture content throughout the pile. High and low moisture materials can be mixed uniformly before being accumulated in the pile. The incorporation of oxygen into the pile is controlled by packing the pile in accordance with the moisture content and by covering the pile with an impermeable material. The pile of cellulosic feedstock achieves a temperature from 150 to 170° F., which depletes oxygen and creates acetic acid to facilitate subsequent processing of the feedstock into ethanol. | 06-11-2009 |
20090203087 | Expression of Granular Starch Hydrolyzing Enzymes in Trichoderma and process for producing glucose from granular starch substrates - The present invention relates to filamentous fungal host cells and particularly | 08-13-2009 |
20090221041 | METHODS FOR STARCH HYDROLYSIS - The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of α-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of a-amylase enzyme or is provided in the form of a purified or partially-purified α-amylase enzyme preparation. The second or subsequent class(es) of α-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified α-amylase enzyme preparation. | 09-03-2009 |
20100068768 | ENZYME COMPOSITIONS AND METHODS FOR THE IMPROVED ENZYMATIC HYDROLYSIS OF CELLULOSE - A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises partially hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one β-glucosidase enzyme and a binding agent for binding the β-glucosidase enzyme to fiber solids present in the aqueous slurry. The unhydrolyzed fiber solids are then separated from the hydrolyzed slurry. The separated fiber solids thus obtained are then re-suspended in an aqueous solution to produce a re-suspended slurry. The hydrolysis is then continued to produce the hydrolysis product comprising glucose. | 03-18-2010 |
20100129880 | HOSTS AND FERMENTATION PROCESSES FOR CELLULASE PRODUCTION - A fermentation process for the production of cellulase mixtures is provided. The process comprises providing a genetically modified host filamentous fungus that overexpresses a Xyr1 transcription factor and/or that is partially or completely deficient in expressing one or more hemicellulase enzyme. The host filamentous fungus is cultured in a medium comprising a carbon source. The carbon source contains from about 60 wt % to about 100 wt % hemicellulose-derived carbohydrate and less than 5% of a cellulase-inducing carbohydrate or contains from about 25 wt % to about 100% wt % hemicellulose-derived sugar alcohol in combination with from about 0 wt % to about 75 wt % glucose, glycerol or a combination thereof. | 05-27-2010 |
20100159535 | METHODS FOR INCREASING HYDROLYSIS OF CELLULOSIC MATERIAL - The present invention relates to methods for pretreating a cellulosic material, methods for degrading or converting a cellulosic material, methods for producing a fermentation product, and methods of fermenting a cellulosic material under anaerobic conditions. | 06-24-2010 |
20110020880 | COMPOSITIONS AND METHODS FOR GRAIN PROCESSING WITHOUT PH ADJUSTMENT - Described are compositions and methods relating to starch processing without a phytase pretreatment step and without adjustment of the slurry pH adjustment. | 01-27-2011 |
20110027837 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. | 02-03-2011 |
20110039307 | ETHANOL YIELDS IN FERMENTATION FROM AN IMPROVED LIQUEFACTION PROCESS - An improved liquefaction process comprises the use of an increased dose of alpha-amylase coupled with a liquefaction temperature no higher than 99° C., e.g., in the range of about 88-92° C. The improved liquefaction process advantageously omits the conventional high-temperature treatment, e.g., jet-cooking at a temperature of about 95-125° C., so that starch can be processed more economically into ethanol, for example. In one embodiment, the improved liquefaction process results in an increased yield of ethanol using commercially available alpha-amylases. | 02-17-2011 |
20110136182 | SWOLLENIN COMPOSITIONS AND METHODS OF INCREASING THE EFFICIENCY OF A CELLULASE - Described are compositions and methods relating to enhancing the efficiency of cellulases for sugar production from cellulosic biomass using the polypeptide swollenin. | 06-09-2011 |
20110171694 | Alpha-Amylase Mutants with Altered Properties - The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca | 07-14-2011 |
20110183379 | FLOW PROCESS FOR PRETREATMENT OF LIGNOCELLULOSIC BIOMASS - Described are methods for pretreating lignocellulosic biomass that comprise thermally conditioning the biomass by flow processing an aqueous slurry of the biomass through an outer passage(s) of one or more heat exchange devices while circulating a heat exchange fluid through an inner passage(s) of the heat exchange device(s). Also described are methods for producing fermentation products, especially ethanol, from the pretreated biomass. | 07-28-2011 |
20110207182 | Novel thermophilic cellobiohydrolase - The present invention provides for a composition comprising a polypeptide comprising a first amino acid sequence having at least 70% identity with the amino acid sequence of Csac GH5 wherein said first amino acid sequence has a thermostable or thermophilic cellobiohydrolase (CBH) or exoglucanase activity. | 08-25-2011 |
20110212495 | COMPARTMENTALIZED SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF BIOMASS - Methods and apparatus' are disclosed for the simultaneous saccharification and fermentation of biomass providing for the compartmentalization of the saccharification process and the fermentation process resulting in decreased enzymatic end-product inhibition. | 09-01-2011 |
20110223639 | Process for Conversion of Granular Starch to Ethanol - The present invention concerns a method of producing glucose from a granular starch substrate comprising, contacting a slurry comprising granular starch obtained from plant material with an alpha-amylase at a temperature below the starch gelatinization temperature of the granular starch to produce oligosaccharides and hydrolyzing the oligosaccharides to produce a mash comprising at least 20% glucose and further comprising fermenting the mash to obtain ethanol. | 09-15-2011 |
20110250652 | PROCESS FOR PRODUCTION OF AN ENZYMATIC PREPARATION FOR HYDROLYSIS OF CELLULOSE FROM LIGNOCELLULOSIC RESIDUES AND APPLICATION THEREOF IN THE PRODUCTION OF ETHANOL - The process of the present invention is based on the microbial production of enzymes from the growth of the fungus | 10-13-2011 |
20110300585 | Systems And Methods For Altering Rates Of Enzymatic Processes - The various embodiments of the present invention relate generally to compositions, systems, and methods for altering rates of catalysis. More particularly, the various embodiments of the present invention are directed toward compositions, systems, and methods for enzymatic hydrolysis of polysaccharides, such as cellulose and starch. An aspect of the present invention comprises a method for altering the rate of conversion of a substrate into a product comprising: providing a substrate in a carrier; mixing a reactant and a co-factor with the carrier to form a substantially homogeneous mixture of the reactant, the co-factor, and the substrate in the carrier; and reacting the reactant with the substrate in the presence of the co- factor to convert at least a portion of the substrate into the product, wherein the reaction rate of the reactant with the substrate in the presence of the co-factor is different than the reaction rate of the reactant with the substrate in the absence of the co-factor. | 12-08-2011 |
20110300586 | Two-Stage Process for Biomass Pretreatment - Described herein are improved methods of pretreating lignocellulosic biomass. One aspect of the invention relates to a two-stage pretreatment process comprising a relatively low severity steam treatment, a controlled pH pretreatment, or autohydrolysis, followed by hydrolysis with dilute acid or hot water at a relatively low temperature. In certain embodiments, the methods increase hemicellulose sugar yields, substrate digestibility, and suitability for fermentation as compared to steam explosion or acid hydrolysis alone. The two-stage pretreatment processes also employ few chemicals, minimizing the costs associated with pretreatment of lignocellulosic biomass. Moreover, the two-stage pretreatment process may expand the range of suitable feedstocks for bioethanol production. | 12-08-2011 |
20120003701 | Heterologous Expression of Termite Cellulases Yeast - The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast | 01-05-2012 |
20120003702 | PROCESS FOR PRODUCING SACCHARIFIED SOLUTION OF LIGNOCELLULOSIC BIOMASS - A process for producing a saccharified solution, by which xylose can be produced at a high yield from lignocellulosic biomass, is provided. The saccharification is carried out by adding a saccharifying enzyme to a pretreated material for saccharification of the lignocellulosic biomass. As the saccharifying enzyme is added a mixture of a first saccharifying enzyme originated from one or more microorganisms selected from the group consisting of fungi of the genus | 01-05-2012 |
20120003703 | CELLOBIOHYDROLASE VARIANTS - The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials. | 01-05-2012 |
20120003704 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. | 01-05-2012 |
20120015407 | Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 01-19-2012 |
20120015408 | BETA-GLUCOSIDASE VARIANT ENZYMES AND RELATED POLYNUCLEOTIDES - The invention provides variants of the | 01-19-2012 |
20120040408 | PROCESSING CELLULOSIC BIOMASS - Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol are disclosed herein, along with plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols than are typically employed are also disclosed. | 02-16-2012 |
20120040409 | Heterologous Biomass Degrading Enzyme Expression in Thermoanaerobacterium Saccharolyticum - Thermophilic gram-positive anaerobic host cells, for example | 02-16-2012 |
20120040410 | THERMOHEMICELLULASES FOR LIGNOCELLULOSIC DEGRADATION - Hemicellulase (xylanase) enzymes possessing endo-xylanase, laminarase, mannanase, arabinase and arabinofuranosidase activity are useful to degrade hemicellulose and other substrates to their constituent sugars. | 02-16-2012 |
20120040411 | PROCESS FOR THE HYDROLYSIS OF CELLULOSE - There is disclosed a process for the hydrolysis of cellulose comprising the sequential steps (a) mixing cellulose with a viscosity below 900 ml/g with an aqueous solution to obtain a liquid, wherein particles comprising cellulose in said liquid have a diameter of maximum 200 nm, wherein the temperature of the aqueous solution is below 35° C., and wherein the pH of the aqueous solution is above 12, (b) subjecting the liquid to at least one of the steps: (i) decreasing the pH of the liquid with at least 1 pH unit and (ii) increasing the temperature by at least 20° C., and (c) hydrolyzing the cellulose. Moreover there is disclose glucose manufactured according to the method and ethanol manufactured from the glucose. Advantages include that the cellulose is hydrolyzed faster and to a greater extent after the treatment. The yield is increased. | 02-16-2012 |
20120040412 | AMYLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars. | 02-16-2012 |
20120058523 | TEMPERING OF CELLULOSIC BIOMASS - The present invention is directed to improved systems and methods for reducing costs and increasing yields of cellulosic ethanol. In particular, the present invention provides plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Also provided are methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols that are typically employed. Such methods allow, among other things, reduced costs associated with externally applied lignocellulolytic enzyme polypeptides. | 03-08-2012 |
20120064579 | Altering Enzyme Balance Through Fermentation Conditions - This present disclosure relates to methods for improved production of proteins from a cell culture, particularly to culture components and conditions that can preferentially increase the expression of proteins produced from genes under the control of xylanase gene promoter sequences. The improved methods can be used for the production of enzyme compositions with enhanced xylanase and hemicellulolytic activity. | 03-15-2012 |
20120070864 | Metal Compounds to Eliminate Nonproductive Enzyme Adsorption and Enhance Enzymatic Saccharification of Lignocellulose - Methods and composition for biofuel processing are provided. For example, in certain aspects methods for using metal compounds to enhance cellulose enzymatic hydrolysis are described. Furthermore, the invention provides integrated processes with fewer lignin removal steps to improve efficiency. | 03-22-2012 |
20120083019 | Use of Glycoside Hydrolase 61 Family Proteins in Processing of Cellulose - The invention provides recombinant GH61 proteins obtained from | 04-05-2012 |
20120088277 | Glucoamylase Variants - The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates. | 04-12-2012 |
20120107880 | FUNGAL STRAINS - The present invention provides improved fungal strains. In some embodiments, the improved fungal strains find use in hydrolyzing cellulosic material to glucose. | 05-03-2012 |
20120122162 | ENZYMATIC HYDROLYSIS OF PRE-TREATED BIOMASS - This disclosure relates to a method for performing high solids saccharification comprising by (a) providing a cellulosic biomass; (b) pretreating the cellulosic biomass in a pretreatment process to produce a pretreated cellulosic biomass; (c) adjusting said pretreated cellulosic biomass to a solids concentration of 6% to 35% w/w and a starting pH of between 5-7; and (d) hydrolyzing the pretreated biomass with at least one aqueous hydrolyzing liquid comprising at least one enzyme selected from the group consisting of a cellulase, a saccharification enzyme, and a combination thereof for a period of time, to hydrolyze at least a part of the pretreated cellulosic biomass to a cellulosic hydrolysate, said cellulosic hydrolysate comprising fermentable sugars. | 05-17-2012 |
20120129226 | TRICHODERMA REESEI ALPHA-AMYLASE IS A MALTOGENIC ENZYME - A maltogenic a-amylase from | 05-24-2012 |
20120129227 | THERMOSTABLE CELLULASES, AND MUTANTS THEREOF, CAPABLE OF HYDROLYZING CELLULOSE IN IONIC LIQUID - The present invention provides for a composition comprising an ionic liquid and a thermostable cellulose, and a method of hydrolyzing a cellulose, comprising: (a) providing a composition comprising a solution comprising an ionic liquid and a cellulose, and (b) introducing a thermostable cellulase to the solution, such that the cellulose is hydrolyzed by the cellulase. The present invention also provides for a | 05-24-2012 |
20120135474 | HEMICELLULOSE-DEGRADING ENZYMES - The present disclosure provides methods for the conversion of hemicellulose into fermentable sugars using enzymes isolated from | 05-31-2012 |
20120135475 | LIQUEFIED BIOMASS - The invention is directed to a liquefied sugar beet and/or sugar cane biomass material as well as production methods and uses thereof. The liquefied biomass is storage stable and can be used for the production of a product resulting from fermentation. | 05-31-2012 |
20120149070 | VARIANTS OF GLUCOAMYLASE - The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells. | 06-14-2012 |
20120156733 | GEOBACILLUS STEAROTHERMOPHILUS ALPHA AMYLASE (AMYS) VARIANTS WITH IMPROVED PROPERTIES - Described are variants of a parent α-amylase that exhibits an alteration in at least one of the following properties relative to said parent α-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production. | 06-21-2012 |
20120164694 | METHOD OF PRODUCING SACCHARIDES CONTAINING GLUCOSE AS MAJOR CONSTITUENT - A method of producing saccharides containing glucose as the major constituent by degrading at least one selected from the group consisting of cellulose and hemicellulose with a cellulose saccharifying enzyme is provided. The method includes the steps of: mixing a cellulose material and a solution containing cellulose saccharifying enzyme to prepare a mixture; and saccharifying the cellulose material with the saccharifying enzyme. A gross energy density Y (W/m | 06-28-2012 |
20120164695 | COMBINATORIAL VARIANTS OF GLUCOAMYLASE WITH IMPROVED SPECIFIC ACTIVITY AND/OR THERMOSTABILITY - Presently provided are variant glucoamylases displaying altered properties, such as improved thermostability and/or specific activity. Also disclosed are DNA sequences coding for the variants, vectors and host cells incorporating the DNA sequence, enzyme compositions, and methods of using the variants in various applications. | 06-28-2012 |
20120171732 | Non-pressurised pre-treatment, enzymatic hydrolysis and fermentation of waste fractions - The present invention relates to a process for production of fermentation products, including bioethanol by non-pressurised pre-treatment, enzymatic hydrolysis and fermentation of waste fractions containing mono- and/or polysaccharides, having a relatively high dry matter content. The process in its entirety, i.e. from non-pressurised pre-treatment over enzymatic hydrolysis and fermentation to sorting of fermentable and non-fermentable solids can be processed at a relatively high dry matter content in a single vessel or similar device using free fall mixing for the mechanical processing of the waste fraction. | 07-05-2012 |
20120178132 | ENDOGLUCANASES - The present invention relates to variant endoglucanases and particularly endoglucanases having improved properties over wild-type endoglucanase. | 07-12-2012 |
20120190075 | AMYLASE POLYPEPTIDES - This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products. | 07-26-2012 |
20120190076 | RECOMBINANT C1 BETA-GLUCOSIDASE FOR PRODUCTION OF SUGARS FROM CELLULOSIC BIOMASS - The invention relates to expression of a recombinant C1 β-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 β-glucosidase, operably linked to heterologous promoter, under conditions in which β-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said β-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel. | 07-26-2012 |
20120196332 | ENZYME METHOD OF EXTRACTING OILS AND PROTEINS FROM VEGETABLE MATTER IN AN AQUEOUS MEDIUM - Method for extracting oils, proteins and fermentable sugars from vegetable material in an aqueous medium, includes:
| 08-02-2012 |
20120202252 | EFFECT OF RADIATION ON CELLULASE ENZYMES - A method for recycling cellulase enzymes. Also provided is a method for producing fermentable carbohydrates, plant leaf protein, and lignin, by adding a cellulase enzyme complex expressed from and on irradiated cellulase complex-producing organisms with sufficient radiation to kill biological activity without destroying all cellulase enzyme complex activity to biomass. The fermentable carbohydrates produced by the method. Also provided are irradiated cellulase-producing organisms for use in converting biomass to fermentable sugars, plant leaf protein, and lignin. A method for producing cellulase enzymes for glucose and other sugar production and protein and lignin extraction by irradiating cellulase-producing organisms, thereby producing the cellulase enzymes is also provided. A system for producing fermentable carbohydrates, plant protein, and lignin, said system comprising irradiated cellulase-producing organisms and biomass is provided. | 08-09-2012 |
20120202253 | ALCOHOL SULFITE BIOREFINERY PROCESS - A biorefinery process to fractionate lignocellulosic materials into cellulose, hemicelluloses and lignin using a pretreatment with mixture of alcohol, sulfur dioxide and water. Further treatment with enzymes, micro-organisms, and optionally bisulfite ion, are used to convert intermediate products to alcohol and lignin derivatives. | 08-09-2012 |
20120202254 | METHOD FOR PRODUCING LACTIC ACID BY NON-STERILE FERMENTATION - The present invention relates to a method for producing lactic acid from plant biomass without requiring sterilization, and specifically relates to a method for producing lactic acid comprising culturing alkaliphilic lactic acid bacteria under non-sterile condition and at a pH of 9 to 11 in a medium containing a cellulose glycosylation solution and then further culturing the bacteria at a pH of 5 to 9. | 08-09-2012 |
20120214210 | B-GLUCANASE AND XYLANASE PREPARATION METHOD USING WHEAT BRAN, AND LIQUID CULTURE MEDIUM - To produce cellulase having excellent ability to decompose cellulosic resources containing xylan at low cost. | 08-23-2012 |
20120231510 | MULTI-CELLULASE ENZYME COMPOSITIONS FOR HYDROLYSIS OF CELLULOSIC BIOMASS - The invention relates to a multi-cellulase enzyme composition for the enzymatic hydrolysis of cellulosic biomass said composition comprising a cellobiohydrolase (CBH) enzyme, an endoglucanase (EG) enzyme and a β-glucosidase (BG) enzyme. | 09-13-2012 |
20120237983 | METHOD FOR INTRODUCING CELLULASE ENZYME TO LIGNOCELLULOSIC FEEDSTOCK SLURRY - The present invention provides a method for producing glucose from a lignocellulosic feedstock. The method comprises the steps of (i) providing a pretreated lignocellulosic feedstock slurry; (ii) moving the pretreated feedstock slurry through a pipe, which slurry has an undissolved solids content of between about 15 and about 30 wt %; (iii) adding cellulase enzyme to the pretreated slurry to produce a slurry comprising cellulase enzyme; (iv) dispersing the cellulase enzyme added in step (iii) in the pretreated slurry by using a high shear in-line mixing device, thereby producing a pretreated slurry comprising dispersed cellulase enzyme; and thereafter (v) subjecting the pretreated slurry comprising dispersed cellulase enzyme to hydrolysis so as to produce glucose from cellulose contained therein. | 09-20-2012 |
20120237984 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. | 09-20-2012 |
20120244579 | PROCESS FOR PRODUCTION OF MONOSACCHARIDE - A monosaccharide production method of producing a monosaccharide from a lignocellulosic raw material comprising: obtaining a saccharified liquid obtained from a lignocellulosic raw material and a saccharification enzyme; recovering the saccharification enzyme from the saccharified liquid by allowing the saccharification enzyme to be adsorbed on the lignocellulosic raw material; and saccharifying the lignocellulosic raw material using the recovered saccharification enzyme. | 09-27-2012 |
20120258503 | CONSOLIDATED PRETREATMENT AND HYDROLYSIS OF PLANT BIOMASS EXPRESSING CELL WALL DEGRADING ENZYMES - Methods for consolidated pretreatment and hydrolysis of genetically engineered plants expressing cell wall degrading enzymes are provided. Expression cassettes and vectors for making transgenic plants are described. Plants engineered to express one or more cell wall degrading enzymes using expression cassettes and vectors of the invention are also provided. | 10-11-2012 |
20120264176 | Alpha-Amylases - The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases. | 10-18-2012 |
20120270275 | SYSTEMS AND METHODS FOR TREATING BIOMASS AND CALCULATING ETHANOL YIELD - The present invention provides processes, inter alia, for the treatment of a starch-based feedstock. The processes include mixing together a starch-based feedstock and a working fluid to form a slurry, hydrating the starch-based feedstock with the working fluid, adding an enzyme to the slurry, pumping the slurry into a substantially constant diameter passage of a fluid mover, and injecting a high velocity transport fluid into the slurry through one or more nozzles communicating with the passage, thereby further hydrating and heating the starch-based feedstock and dispersing the starch content of the slurry. Apparatuses for carrying out such processes are also provided. Processes for converting starch in feedstocks into oligosaccharides and systems for producing sugars and ethanol using the processes and apparatuses of the invention are also provided. Processes for calculating ethanol yield using the apparatuses are also provided. | 10-25-2012 |
20120270276 | METHOD FOR PRODUCING SACCHARIDES CONTAINING GLUCOSE AS MAIN COMPONENTS - Provided is a method for producing saccharides containing glucose as the main components which can increase the generated amount of saccharides containing glucose as the main components even when an enzymatic saccharification reaction is performed with a small amount of enzyme. A method for producing saccharides containing glucose as the main components is provided which includes mixing biomass containing cellulose and/or hemicellulose and an aqueous enzyme solution, and then performing an enzymatic saccharification reaction with an enzyme contained in the aqueous enzyme solution while maintaining the state where the mixture of the biomass containing cellulose and/or hemicellulose and the aqueous enzyme solution is allowed to stand still in a reaction vessel. | 10-25-2012 |
20120270277 | Biomass Hydrolysis Process - The invention relates to a biomass process comprising split dosage of the cellulose hydrolysing enzyme. | 10-25-2012 |
20120276594 | CELLOBIOHYDROLASE VARIANTS - The present invention relates to cellobiohydrolase variants having improved thermostability and/or thermoactivity in comparison to wild-type | 11-01-2012 |
20120276595 | COMPOSITIONS AND METHODS COMPRISING CELLULASE VARIANTS WITH REDUCED AFFINITY TO NON-CELLULOSIC MATERIALS - The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions. | 11-01-2012 |
20120288900 | Method of Producing Corn Starch by Enzymatic Process - A method of producing corn starch by enzymatic process involving: soaking the corn; crushing the corn, separating and washing embryo; fine grinding; washing and drying fiber; separating and drying protein; washing, dewatering and drying the starch. An enzyme preparation is added before the step of washing, dewatering and drying the starch; the enzyme preparation is cellulose, or xylanase, or combination of the cellulose and the xylanase; and addition of the enzyme preparation is from 0.001% to 0.08% by weight of the corn. Based on the technology of traditional wet process, the method of the present invention comprises a step of adding enzyme preparation in the process of separating the corn, which improves the effect and the efficiency of mechanical separation, and further improves the purity and yield of the substance separated while also reducing the energy consumption. | 11-15-2012 |
20120301928 | PREPROCESSING METHOD OF HYDROLYZING LIGNOCELLULOSIC BIOMASS AND METHODS OF MANUFACTURING SUGAR COMPOUND AND BIOETHANOL FROM BIOMASS PROCESSED USING THE PREPROCESSING METHOD - The present invention relates to a method of pretreating lignocellulosic biomass prior to hydrolysis, and, more particularly, to a method of pretreating lignocellulosic biomass prior to hydrolysis using wet milling in combination with popping, a method of manufacturing a sugar compound from the biomass pretreated using the method, and a method of manufacturing bioethanol from the biomass pretreated using the method. According to the present invention, energy consumption can be reduced, lignocellulosic biomass can be easily pretreated in an environment-friendly manner without treating chemicals, and the efficiency of hydrolyzing lignocellulosic biomass can be remarkably improved. | 11-29-2012 |
20120301929 | METHOD FOR DEGRADING ORGANIC MATERIAL USING MOTHER CELL LYASES FORMED IN ASSOCIATION WITH SPORE FORMATION OF MICROORGANISM - Provided is a method for degrading an organic material. Also provided is a useful low-molecular organic material. The method for degrading an organic material is characterized by comprising a step for preparing the organic material to be degraded, and a step for treating said organic material with mother cell lytic enzymes which are formed through cytolysis associated with the spore formation of a spore-forming aerobic bacterium. | 11-29-2012 |
20120301930 | BIO-ENGINEERED MULTI-ENZYME COMPLEXES COMPRISING XYLANASES AND USES THEREOF - The present invention relates to bio-engineered multi-enzyme complexes having synergistic enzyme activity comprising xylanases and optionally further comprising additional carbohydrate active enzymes. Such complexes are advantageous for degrading recalcitrant cellulosic biomass. | 11-29-2012 |
20120309059 | Polypeptides Having Endoglucanase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 12-06-2012 |
20120315674 | METHODS OF ENZYMATIC HYDROLYSIS - In one embodiment the instant invention generally pertains to a method for producing glucose for fermentation. The method comprises first treating a biomass comprising a lignocellulosic material with a mixture comprising SO2 and steam at reaction conditions sufficient to produce a composition mixture comprising cellulose suitable for enzymatic hydrolysis. Specifically, the temperature, residence time, and SO2 concentration may be selected by calculating a crystallinity index (CrI) of the biomass and using the calculated crystallinity index as an indicator of enzymatic hydrolysis rate. In this manner cellulose may be enzymatically hydrolyzed glucose for aerobic or anaerobic fermentation. | 12-13-2012 |
20120315675 | PROCESSING BIOMASS - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce a product such as a biofuel. | 12-13-2012 |
20120315676 | ENDOGLUCANASES - The present invention relates to variant endoglucanases and particularly endoglucanases having improved properties over wild-type endoglucanase. | 12-13-2012 |
20120329099 | Modified Algae for Improved Biofuel Productivity - Methods, processes, and systems for the production of lipids and starch from modified algae are disclosed. In one embodiment, the modified algae over-expresses isoamylase and accumulates much higher amounts of starch than unmodified algae. In some embodiments, the modified algae comprises one or more copies of an isoamylase expression construct. In one embodiment, the modified algae is a sta7 | 12-27-2012 |
20120329100 | LIGNIN-BASED ENZYME STABILIZER - The invention relates to an enzyme stabilizer comprising a lignin derivative produced by reaction between lignin and a hydrophilic compound, and to a method of saccharifying lignocellulosic biomass which employs the enzyme stabilizer. According to the invention it is possible to accomplish effective saccharification of cellulosic biomass with saccharifying enzymes, by enhancing saccharifying enzyme activity and preventing nonspecific adsorption of saccharifying enzyme onto substrate. | 12-27-2012 |
20120329101 | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 12-27-2012 |
20130004997 | METHOD FOR PRODUCING SACCHARIFIED SOLUTION - A method for producing a saccharified solution is provided, by which a saccharide recovered as a saccharified solution can be increased. The saccharified solution is obtained by treating a substrate solution containing lignocellulosic biomass as a substrate with a saccharifying enzyme produced by a microorganism to prepare a substrate/saccharifying enzyme mixture liquid, and removing a residue of the substrate from the substrate/saccharifying enzyme mixture liquid. The concentration of the substrate in the substrate/saccharifying enzyme mixture liquid is adjusted to be in the range of 15 to 30% by mass. In the removal of the residue of the substrate, a saccharide adsorbed on the residue is extracted. | 01-03-2013 |
20130011882 | ALPHA-AMYLASE VARIANTS WITH ALTERED PROPERTIES - Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto. | 01-10-2013 |
20130011883 | NATIVE GRAIN AMYLASES IN ENZYME COMBINATIONS FOR GRANULAR STARCH HYROLYSIS - Described herein are starch hydrolysis processes for obtaining fermentable sugars from starch in milled plant material at temperatures below the starch gelatinization temperature and using exogenous plant alpha amylases further to the fermentation of the sugars to produce end products, such as ethanol. | 01-10-2013 |
20130017579 | BIPHASIC SUPERCRITICAL CARBON DIOXIDE-WATER PRETREATMENT OF LIGNOCELLULOSIC BIOMASS - A process for pretreatment of a biomass material, comprising subjecting a biomass material to a biphasic mixture of water and supercritical CO | 01-17-2013 |
20130023013 | METHOD FOR OBTAINING A FERMENTABLE PRODUCT FROM POLLUTED LIGNO-CELLULOSIC BIOMASS - A method for obtaining a fermentable product intended for the production of metabolites by fermentation, includes the following steps: a) detoxification of a polluted ligno-cellulosic raw material including at least one polluting component; solid-liquid extraction in order to extract the polluting component(s) from the ligno-cellulosic raw material by solubilisation using at least one solvent; recovery of the cleaned ligno-cellulosic raw material; b) pre-treatment of the cleaned ligno-cellulosic raw material by defibration; c) enzymatic hydrolysis of the pre-treated ligno-cellulosic raw material in the presence of cellulose, and obtaining a fermentable product; d) optional purification of the resulting fermentable product. The use of the resulting fermentable product for producing metabolites by fermentation, as well as to a specific method for producing ethanol from the fermentable product are also described. | 01-24-2013 |
20130023014 | NOVEL CELLULASE GENE - An object is to identify endoglucanase and β-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or β-glucosidases, from | 01-24-2013 |
20130034883 | Processes of Producing a Fermentation Product - The invention relates to a process of process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of a GH61 polypeptide; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism. | 02-07-2013 |
20130040345 | HOST CELL CAPABLE OF PRODUCING ENZYMES USEFUL FOR DEGRADATION OF LIGNOCELLULOSIC MATERIAL - The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamentous fungus and is capable of secretion of the at least four different enzymes. | 02-14-2013 |
20130040346 | Cellobiohydrolase Variants and Polynucleotides Encoding Same - The present invention relates to variants of a parent cellobiohydrolase. The present invention also relates to polynucleotides encoding the cellobiohydrolase variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the cellobiohydrolase variants. | 02-14-2013 |
20130045509 | ENZYMATIC HYDROLYSIS PRETREATMENT OF LIGNOCELLULOSIC MATERIALS - This disclosure relates to a method for treating a biomass comprising a lignocellulosic material to produce fermentable sugars comprising the steps of treating the biomass to produce a biomass with a depolymerized lignin, adding a carbonyl scavenger to the biomass before, during, or after said treating step to inhibit repolymerization of the lignin, adding at least one of a laccase enzyme and a cellulases enzyme to the biomass with depolymerized lignin subsequently to the addition of the carbonyl scavenger, and producing a fermentable sugar from the action of the laccase enzyme and cellulases enzyme on the biomass with depolymerized lignin. | 02-21-2013 |
20130045510 | Novel Beta-Glucosidase and Uses thereof - A novel beta-glucosidase and nucleic acids encoding the beta-glucosidase. Also disclosed are cells, compositions, and methods relating to using the beta-glucosidase to convert ligocellulosic material to fermentable sugars. | 02-21-2013 |
20130052697 | METHOD FOR HYDROLYTIC PRE-TREATMENT OF LIGNOCELLULOSIC AND PERENNIAL HERBACEOUS BIOMASS AND FOR PRODUCTION OF SACCHARIDE AND BIOETHANOL FROM THE PRE-TREATED BIOMASS - Disclosed is a method for the hydrolytic pre-treatment of lignocellulosic and perennial herbaceous biomass. By the method, a material suitable for use in the production of saccharides and biofuels can be prepared from lignocellulosic biomass such as pine wood and oak tree wood and perennial herbaceous biomass such as flame grasses and reeds. It is characterized by wet-triturating, microwaving and popping processes. Also, a method is provided for the production of saccharides and bioethanol from the pre-treated biomass. | 02-28-2013 |
20130059345 | MANUFACTURING METHOD FOR SUGAR SOLUTION AND DEVICE FOR SAME - A method of hydrolysis of cellulose uses a filamentous fungus-derived cellulase as a carbohydrase and includes adding the carbohydrase to cellulose to perform primary hydrolysis and then subjecting the primary hydrolysate to solid-liquid separation into a primary sugar liquid and solids; adding water to the solids and performing secondary hydrolysis, followed by subjecting the secondary hydrolysate to solid-liquid separation into a secondary sugar liquid and a residue; and filtering the primary sugar liquid and/or secondary sugar liquid through an ultrafiltration membrane, and recovering the carbohydrase from the feed side and recovering a sugar solution from the permeate side. | 03-07-2013 |
20130059346 | Conversion System For Biomass - The efficient production of ethanol from low-cost biomass (e.g., corn, sugar beets, sugar cane, switchgrass and/or paper) has become increasingly important in making ethanol competitive with gasoline and decreasing the United States' dependence on foreign oil. For example, to reduce the cost of transporting biomass to ethanol production facilities, mobile systems for producing ethanol from biomass are provided. Also provided are small-scale ethanol production facilities. For example, instead of transporting biomass to the production facility, the facility is transported to the biomass or is located nearby the source of the biomass. The ethanol production facilities or components thereof may be transported via land, water, or air. Production of other products, such as hydrocarbons, natural gas, hydrogen gas, plastics, polymers, and proteins, can also be made by the methods and facilities. Any product described herein can be made in finished form or un-finished form and moved, e.g., to a fixed facility, e.g., fixed production facility. | 03-07-2013 |
20130059347 | SACCHARIFIED-SOLUTION MANUFACTURING METHOD AND SACCHARIFIED-SOLUTION MANUFACTURING DEVICE USED IN SAID METHOD - [Problems to be Solved] | 03-07-2013 |
20130071884 | PLANTS EXPRESSING CELL WALL DEGRADING ENZYMES AND EXPRESSION VECTORS - Vectors for expression of proteins in plants are described. The proteins may be enzymes and the enzymes can be but are not limited to cell wall degrading enzymes. A number of plants designed to express specific cell wall degrading enzymes are provided. The plants may have industrial and/or agricultural applications. Methods and materials for making the expression vectors and for making the plants are provided. Processes for which the plants could be used in industrial and agricultural applications are also provided. | 03-21-2013 |
20130078677 | PROCESS FOR THE PRODUCTION OF CARBOHYDRATE CLEAVAGE PRODUCTS FROM A LIGNOCELLULOSIC MATERIAL - A process for the production of carbohydrate cleavage products, characterized by a combination of the measures that a lignocellulosic material is treated with an aqueous solution containing an alcohol, in particular a C | 03-28-2013 |
20130078678 | Protozoan Glycosidases and Related Methods - Nucleic acids encoding glycosidases useful for the hydrolysis of cellulose and hemicellulose obtained from ciliates residing in a bovine rumen are provided. Also provided recombinant nucleic acids encoding the glycosidases, transformed cells comprising the same and related methods of using the transformed cells and glycosidases to degrade cellulose and/or hemicellulose. | 03-28-2013 |
20130078679 | Polypeptides Having Cellobiohydrolase 1 Activity and Polynucleotides Encoding Same - The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. | 03-28-2013 |
20130078680 | METHOD FOR PRODUCTION OF FERMENTABLE SUGARS FROM BIOMASS - A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process. | 03-28-2013 |
20130084608 | FUNGAL PROTEASES - The present invention provides fungal proteases and improved fungal strains that are deficient in protease production. | 04-04-2013 |
20130095530 | BETA-GLUCOSIDASE VARIANT ENZYMES AND RELATED POLYNUCLEOTIDES - The invention provides variants of the | 04-18-2013 |
20130095531 | POLYPEPTIDE HAVING BETA-GLUCOSIDASE ACTIVITY AND USES THEREOF - The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 70% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 70% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins. | 04-18-2013 |
20130109062 | THERMOSTABLE XYLANASE FOR THE SELECTIVE HYDROLYSIS OF PENTOSE-CONTAINING POLYSACCHARIDES | 05-02-2013 |
20130115659 | COMPOSITIONS AND METHODS FOR GRAIN PROCESSING WITHOUT PH ADJUSTMENT - Described are compositions and methods relating to starch processing without a phytase pretreatment step and without adjustment of the slurry pH adjustment. | 05-09-2013 |
20130122554 | METHOD FOR TREATING LIGNOCELLULOSIC BIOMASS - It is intended to provide a method for treating lignocellulosic biomass, which can reliably show the completion of the course by which pretreated lignocellulosic biomass is rendered flowable and thus transportable. The method for treating lignocellulosic biomass comprises a first saccharification step of saccharifying pretreated lignocellulosic biomass with stirring using a stirring unit | 05-16-2013 |
20130122555 | BIOMASS HYDROTHERMAL DECOMPOSITION SYSTEM AND SACCHARIDE-SOLUTION PRODUCTION METHOD USING BIOMASS MATERIAL - A saccharide-solution production method using a biomass material, including feeding a biomass material containing cellulose, hemicellulose, and lignin under a normal pressure to put it under an increased pressure; hydrothermally decomposing the biomass material using pressurized hot water by a hydrothermal decomposition unit; and dissolving a lignin component and a hemicellulose component in the pressurized hot water; thereafter, adding a biomass solid discharged from the hydrothermal decomposition unit to a slurrying vessel containing water injected therein and communicating with the hydrothermal decomposition unit so as to obtain a slurried biomass solid; then, removing water from the slurried biomass solid; and thereafter, performing enzymatic saccharification of the water-removed biomass solid to produce a saccharide solution. | 05-16-2013 |
20130130325 | Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase and Beta-Xylosidase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 05-23-2013 |
20130130326 | Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase and Beta-Xylosidase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 05-23-2013 |
20130130327 | Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase and Beta-Xylosidase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 05-23-2013 |
20130130328 | METHOD OF TREATING PLANT BIOMASS - Plant biomass is immersed in a solution that contains a polar solvent and an imidazolium salt that has a melting point of at least 100° C. As a result, the cellulose and hemicellulose present in the plant biomass are relaxed (decrystallized and depolymerized) and brought into an easy-to-degrade state. Reacting the immersed plant biomass with a cellulase produces saccharide at a high conversion efficiency. | 05-23-2013 |
20130137147 | METHOD FOR PRODUCTION OF FERMENTABLE SUGARS FROM BIOMASS - A process for production of fermentable sugars from biomass using multi-enzyme multi-step system is provided herein. The process disclosed in the present invention provides high yielded sugars in less time period. The multi-enzyme system disclosed in the present invention converts celluloses, hemicelluloses and/or mixture thereof to fermentable sugar with higher efficiency and better economics than the process known in the prior art. Cellulose and hemicelluloses fractions derived from natural sources such as any lignocellulosic biomass are saccharified in a shortened time with higher conversion rates of intermediates with modified enzymatic compositions/groups of the Multi-enzyme system to enhance the rate thus providing an economical cellulose and hemicellulose saccharification process. | 05-30-2013 |
20130143277 | Methods for Improving the Efficiency of Simultaneous Saccharification and Fermentation Reactions - The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions. | 06-06-2013 |
20130143278 | RECYCLE OF LEACHATE DURING LIGNOCELLULOSIC CONVERSION PROCESSES - The present invention provides a process for producing fermentable sugar or a fermentation product from a lignocellulosic feedstock. The process comprises leaching the lignocellulosic feedstock with an aqueous solution to remove at least potassium salts from the lignocellulosic feedstock and without significantly hydrolyzing hemicellulose and cellulose, thereby producing a leached feedstock and leachate. The leachate is removed from the leachate and concentrated. The leached feedstock is hydrolyzed to produce fermentable sugar, which may be fermented to produce a fermentation broth comprising the fermentation product. The concentrated leachate is recirculated to one or more stages in the process involving alkali addition to adjust the pH of a process stream. | 06-06-2013 |
20130149750 | METHOD FOR PRETREATMENT OF CELLULOSE FOR FERMENTATION IN AN AQUEOUS ENVIRONMENT - A method for pretreating cellulose for fermentation in an aqueous environment comprises the steps of: grinding a biomass to reduce its particle size, adding water to the ground biomass to create a slurry, soaking the slurry, heating the slurry at a first pressure greater than atmospheric pressure, reducing the pressure of the slurry, reducing the temperature of the slurry, determining whether the enzymes used require preliminary enzymatic hydrolysis, and performing preliminary enzymatic hydrolysis on the slurry if the enzymes used require preliminary enzymatic hydrolysis. | 06-13-2013 |
20130157317 | Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Dioxy Compound And Uses Thereof - The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a dioxy compound. The present invention also relates to methods of using the compositions. | 06-20-2013 |
20130157318 | METHOD FOR ENZYMATIC SACCHARIFICATION TREATMENT OF LIGNOCELLULOSE-CONTAINING BIOMASS, AND METHOD FOR PRODUCING ETHANOL FROM LIGNOCELLULOSE-CONTAINING BIOMASS - A method for the enzymatic saccharification of a lignocellulosic raw material, including adding a pretreated lignocellulosic raw material as a material suitable for an enzymatic saccharification reaction, together with an electrolyte containing a water-soluble salt, to water that contains a celluolose saccharification enzyme; saccharifying the raw material by an enzymatic saccharification reaction, as a suspension of the raw material having an electrical conductivity adjusted to 5-25 mS/cm; separating and recovering a reaction product and an enzyme-containing solution from the enzymatically saccharified treatment suspension; and recycling the recovered enzyme-containing solution as the enzyme for the enzymatic saccharification step. | 06-20-2013 |
20130164791 | Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Quinone Compound And Uses Thereof - The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a quinone compound. The present invention also relates to methods of using the compositions. | 06-27-2013 |
20130171699 | NOVEL ALPHA-NEOAGAROBIOSE HYDROLASE AND METHOD FOR OBTAINING A MONOSACCHARIDE USING SAME - Provided are novel α-neoagarobiose hydrolase and a method for obtaining a monosaccharide using the same. | 07-04-2013 |
20130177947 | NOVEL GLYCOSYL HYDROLASE ENZYMES AND USES THEREOF - The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars. | 07-11-2013 |
20130177948 | CELLULOSE SOLUTION MANUFACTURING METHOD, CELLULOSE PRECIPITATE MANUFACTURING METHOD, CELLULOSE SACCHARIFICATION METHOD, CELLULOSE SOLUTION, AND CELLULOSE PRECIPITATE - The invention relates to a cellulose solution manufacturing method including: performing an ozonation treatment to bring a cellulose-containing material and ozone into contact with each other; and performing an alkali treatment to bring the obtained treated material and an alkali aqueous solution into contact with each other, thereby dissolving at least cellulose in the cellulose-containing material brought into contact with the ozone in the alkali aqueous solution. According to the invention, it is possible to provide a method of manufacturing a cellulose solution in which cellulose can be dissolved in a more simple manner, a method of manufacturing a cellulose precipitate in which cellulose can be recovered from the cellulose solution, and a method of saccharifying cellulose which uses the cellulose precipitate. | 07-11-2013 |
20130183720 | PROCESSING BIOMASS - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material. | 07-18-2013 |
20130183721 | COOLING AND PROCESSING MATERIALS - Systems and methods for cooling and processing materials are disclosed. | 07-18-2013 |
20130183722 | Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Nitrogen-Contaning Compound And Uses Thereof - The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a nitrogen-containing compound. The present invention also relates to methods of using the compositions. | 07-18-2013 |
20130183723 | "Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And An Organic Compound And Uses Thereof" - The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and an organic compound. The present invention also relates to methods of using the compositions. | 07-18-2013 |
20130189744 | Method and Compositions for Improved Lignocellulosic Material Hydrolysis - A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of | 07-25-2013 |
20130189745 | ARTIFICIAL CELLULOSOME AND THE USE OF THE SAME FOR ENZYMATIC BREAKDOWN OF RESILIENT SUBSTRATES - The present invention relates to an in vitro produced, artificial cellulosome for enzymatic breakdown of resilient substrates. In particular, the present invention provides a complex having an increased activity on resilient substrates, such as crystalline cellulose. The in vitro formed complex comprises a backbone scaffold having at least four binding sites capable of binding the enzyme components, whereby at least two of the binding sites have essentially the same binding specificity; and at least three different enzyme components being randomly bound to the at least four binding sites. Method for preparing the complex and uses of the same for enzymatic breakdown of resilient substrates are also provided. | 07-25-2013 |
20130189746 | GALACTO-OLIGOSACCHARIDE-CONTAINING COMPOSITION AND A METHOD OF PRODUCING IT - The present invention relates to a method of producing compositions containing galacto-oligosaccharides as well as to galacto-oligosaccharide-containing compositions as such. | 07-25-2013 |
20130189747 | Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Sulfur-Containing Compound And Uses Thereof - The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a sulfur-containing compound. The present invention also relates to methods of using the compositions. | 07-25-2013 |
20130196386 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. | 08-01-2013 |
20130196387 | Polypeptides Having Endoglucanase Activity And Polynucleotides Encoding Same - The present invention provides isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cell comprising the polynucleotides as well as methods of producing and using the polypeptides. | 08-01-2013 |
20130203124 | Alpha-Amylase Variant With Altered Properties - The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase. | 08-08-2013 |
20130203125 | CELLULOSIC PROCESSING TRAIT DEVELOPMENT USING A THERMOREGULATED, INTEIN-MODIFIED XYLANASE - In planta consolidated bioprocessing has the advantages of decreasing biomass pretreatment costs, utilizing excess plant protein production capacity for enzyme production, and decreasing mass transfer resistance of enzyme diffusion to its substrate. However, in planta expression of cell wall degrading (CWD) enzymes often leads to detrimental plant phenotypes that impact crop yield. To provide in planta CWD enzyme activity without any adverse phenotype, a thermostable xylanase, XynB (EC 3.2.1.8), was engineered with a thermoregulated intein, Tth-HB27 DnaE-1 (Tth intein), that controls its hydrolytic activity through conditional intein splicing. Maize plants expressing the heat inducible Tth intein-modified XynB developed normally, yet possessed enhanced post harvest glucose production from dried corn stover. Expression of CWD enzymes as dormant, intein-modified proteins that can be activated by heat treatment after harvest provides the basis for developing a novel cellulosic processing trait in plants. | 08-08-2013 |
20130203126 | Alpha-Amylases - The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases. | 08-08-2013 |
20130203127 | Methods for Increasing Enzymatic Hydrolysis of Cellulosic Material in the Presence of a Peroxidase - The present invention relates to methods for increasing hydrolysis of a cellulosic material, comprising: hydrolyzing the cellulosic material with an enzyme composition in the presence of a polypeptide having peroxidase activity. | 08-08-2013 |
20130210083 | THERMOSTABLE ENZYME TECHNOLOGY FOR ALGAL BIOCONVERSION - Thermostable enzyme technology for algal bioconversion The present invention relates to thermostable enzyme systems suitable for use in the production of biofuels and bio-products from algae, and to a method of producing energy feedstocks, stocks, specifically (i) fermentable sugars and (ii) lipid fractions from algae, for the production of biofuels such as bioethanol, biobutanol and bio-oils or biodiesel, as well as other value-added biomolecules (e.g. proteins, peptides, oils, pigments, nucleic acids). | 08-15-2013 |
20130210084 | USE OF MAGNESIUM HYDROXIDE FOR PH ADJUSTMENT AND IMPROVED SACCHARIFICATION OF BIOMASS - The present invention provides methods of processing biomass containing a cellulosic material that include contacting the cellulosic material with magnesium hydroxide to adjust the pH of the material before and/or after a high temperature and high pressure pretreatment step. The use of magnesium hydroxide provides a safer alternative to using ammonium hydroxide for pH adjustment, a more stable, buffered environment for improved operability in pH control, with similar or improved conversion of biomass to fermentable sugars, and similar or improved reduction of inhibitors during the subsequent hydrolysis or saccharification process. | 08-15-2013 |
20130210085 | Methods and Systems for Pretreatment of Biomass Solids - A method for the pretreatment of biomass solids includes hydrating the biomass solids to form a biomass slurry, shear treating the biomass solids, and hydrolyzing the biomass solids in the presence of reactive enzymes in a pressure hydrolysis zone. Shear treatment of the biomass solids reduces the particle size of the biomass solids, modifies the particle or slurry morphology, and/or ruptures the cell walls of the biomass solids. The pressure hydrolysis zone includes a high-shear, high-pressure, low-temperature heat exchange and reaction zone and a low-pressure, low-temperature polishing zone. Sugars formed from the biomass solids treated in accordance with the methods described above may be used to produce various biofuels. | 08-15-2013 |
20130210086 | Methods of Degrading or Hydrolyzing a Polysaccharide - The invention provides a method of degrading or hydrolyzing a polysaccharide, preferably cellulose or chitin, comprising contacting said polysaccharide with one or more oxidohydrolytic enzymes, preferably a CBM33 family protein (preferably CBP21) or a GH61 family protein, wherein said degradation or hydrolysis is carried out in the presence of at least one reducing agent and at least one divalent metal ion. A method of producing an organic substance comprising said method is also provided. | 08-15-2013 |
20130210087 | Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same - The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 08-15-2013 |
20130210088 | Enzymatic Hydrolysis of Lignocellulosic Material in the Presence of Sulfite, Dithionite and/or Dithiothreitol - A method is provided for improving enzymatic hydrolysis in saccharification of a lignocellulosic material. The method is comprising pretreating the lignocellulosic material to obtain a slurry of pretreated lignocellulosic material; adding at least one reducing agent to the slurry of pretreated lignocellulosic material or the liquid fraction thereof to decrease the enzymatic hydrolysis inhibitory properties of slurry of the pretreated lignocellulosic material or the liquid fraction thereof; and subjecting the slurry of pretreated lignocellulosic material or the liquid fraction thereof to enzymatic hydrolysis in the presence of the at least one reducing agent. | 08-15-2013 |
20130217072 | Heterologous Expression of Fungal Cellobiohydrolase 2 Genes in Yeast - The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms | 08-22-2013 |
20130217073 | DIGESTIBLE LIGNOCELLULOSIC BIOMASS AND EXTRACTIVES AND METHODS FOR PRODUCING SAME - The invention relates to methods for treating lignocellulosic biomass to obtain useful products therefrom. | 08-22-2013 |
20130217074 | Enzymatic Hydrolysis of Cellulose - The present invention relates to a continuous process for the enzymatic hydrolysis of cellulosic biomass and to an apparatus for conducting said process. According to the present invention, a steady state is achieved in a reactor in regard to the hydrolysis reaction. Therein, cellulosic biomass of a high total solids content (preferably 10% or higher, further preferably between 15 and 30%) is continually added to said reactor, while at least partially hydrolyzed cellulosic biomass is continually removed from said reactor. The steady state is adjusted, i.e. the amount of cellulosic biomass added and the amount of at least partially hydrolyzed cellulosic biomass removed is adjusted, so that the retention time of a given portion of added cellulosic biomass in the reactor is longer than its “liquefaction time”, i.e. the time period required to transform a solid slurry into a pumpable liquid during hydrolysis, i.e. the time required to lower the viscosity of the slurry to a value, which is acceptable for further processing. | 08-22-2013 |
20130217075 | METHOD OF PRODUCING BIOFUEL USING BROWN ALGAE - In a method of producing biofuel using brown algae, | 08-22-2013 |
20130217076 | POLYPEPTIDE CAPABLE OF ENHANCING CELLULOSIC BIOMASS DEGRADATION - The saccharification efficiency of cellulase is enhanced within reaction temperature regions of general fermenting microorganisms other than heat-resistant yeast. Cellulosic biomass is saccharified with cellulase in the presence of a polypeptide comprising the amino acid sequence shown in SEQ ID NO: 2 or 4 and having a function of enhancing the saccharifying activity of cellulase. | 08-22-2013 |
20130217077 | Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Bicyclic Compound And Uses Thereof - The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a bicyclic compound. The present invention also relates to methods of using the compositions. | 08-22-2013 |
20130217078 | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 08-22-2013 |
20130217079 | Beta-Glucosidase Variants and Polynucleotides encoding same - The present invention relates to variants of a parent beta-glucosidase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 08-22-2013 |
20130224800 | Enzymes Having Alpha Amylase Activity and Methods of Making and Using them - The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. | 08-29-2013 |
20130224801 | TREATMENT OF CELLULOSIC MATERIAL AND ENZYMES USEFUL THEREIN - The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations. | 08-29-2013 |
20130224802 | Methods of Hydrolyzing Oligomers in Hemicellulosic Liquor - The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars. | 08-29-2013 |
20130230888 | Heterologous Expression of Fungal Cellobiohydrolase 2 Genes in Yeast - The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms | 09-05-2013 |
20130236933 | Methods for Producing a Fermentation Product from Lignocellulose-Containing Material - The present invention provides a method for producing a fermentation product from lignocellulose-containing material, a method for converting lignocellulose-containing material into a hydrolyzate comprising mono- and oligo-saccharides, and a method for treating lignocellulose-containing material, all of which comprise the step of mixing an acid pre-treated lignocellulose-containing material and an alkaline pre-treated lignocellulose-containing material. The present invention further provides a fermentation product made according to the method for producing a fermentation product. | 09-12-2013 |
20130244289 | VARIANT HUMICOLA GRISEA CBH1.1 - Disclosed are variants of | 09-19-2013 |
20130244290 | PROCESSES AND APPARATUS FOR PRODUCING FERMENTABLE SUGARS AND LOW-ASH BIOMASS FOR COMBUSTION AT REDUCED EMISSIONS - This invention provides processes and apparatus to convert biomass, including wood and agricultural residues, into low-ash biomass pellets for combustion, alone or in combination with another solid fuel. Some embodiments provide processes for producing hemicellulosic sugars and low-ash biomass from cellulosic biomass, comprising providing an aqueous extraction solution with acetic acid; extracting the feedstock to produce an extract liquor containing soluble ash, hemicellulosic oligomers, acetic acid, dissolved lignin, and cellulose-rich solids; dewatering and drying the cellulose-rich, lignin-rich solids to produce a low-ash biomass; hydrolyzing the hemicellulosic oligomers to produce fermentable hemicellulosic sugars, wherein additional acetic acid is generated; removing a vapor stream comprising vaporized acetic acid from the extract; recycling the vapor or its condensate to provide some starting acetic acid for the extraction solution; and recovering fermentable hemicellulosic sugars. The disclosed processes can produce clean power from biomass. Co-products include fermentation products such as ethanol, fertilizers, and lignin. | 09-19-2013 |
20130244291 | PROCESSES AND APPARATUS FOR PRODUCING ENERGY-DENSE BIOMASS FOR COMBUSTION AND FERMENTABLE SUGARS FROM THE BIOMASS - This invention provides processes to convert biomass into energy-dense biomass for combustion, alone or in combination with another solid fuel. Some embodiments provide processes for producing fermentable sugars and energy-dense biomass from cellulosic biomass, comprising extracting the feedstock with steam and/or hot water to produce an extract liquor containing hemicellulosic oligomers, dissolved lignin, and cellulose-rich solids; separating the extract liquor, to produce dewatered cellulose-rich solids; hydrolyzing the dewatered cellulose-rich solids, thereby removing a portion of the cellulose, to produce intermediate solids (with higher energy density) and a hydrolysate; drying the intermediate solids to produce energy-dense biomass; and recovering fermentable sugars from the hydrolysate. The energy-dense biomass may be pelletized into biomass pellets, which may have a similar energy density as torrefied pellets from wood. The hemicellulosic oligomers may be further hydrolyzed to produce additional fermentable sugars. The fermentable sugars may be fermented to ethanol or another product. | 09-19-2013 |
20130244292 | Polypeptides Having Endoglucanase Activity - The present invention relates to a family 5 glycoside hydrolase variant having endoglucanase activity, polynucleotides encoding the family 5 glycoside hydrolase variant, vectors, host cells comprising the polynucleotides, and methods for using the family 5 glycoside hydrolase variant. | 09-19-2013 |
20130244293 | FRACTIONATED EXTRACTIVE PRODUCTS FROM PLANT BIOMASS AND METHODS OF MAKING AND USING SAME - A method is provided comprising converting at least a portion of native cellulose I | 09-19-2013 |
20130244294 | PROCESSING BIOMASS - Methods of manufacturing fuels are provided. These methods use often difficult to process lignocellulosic materials, for example crop residues and grasses. The methods can be readily practiced on a commercial scale in an economically viable manner, in some cases using as feedstocks materials that would otherwise be discarded as waste. | 09-19-2013 |
20130252284 | NOVEL CELLULASE DERIVED FROM THERMOSPOROTHRIX HAZAKENSIS - This invention provides a novel cellulase derived from | 09-26-2013 |
20130252285 | IONIC LIQUID PRETREATMENT OF CELLULOSIC BIOMASS: ENZYMATIC HYDROLYSIS AND IONIC LIQUID RECYCLE - The present invention relates to compositions and methods of pretreating cellulosic biomass with an ionic liquid. More specifically it relates to pretreating cellulosic biomass in an ionic liquid and adding a salt containing a kosmotropic anion to facilitate separation of precipitated solids, the aqueous phase, and the ionic liquid phase. The ionic liquid phase may be recycled in a subsequent pretreatment step, and the precipitated solids are enzymatically hydrolyzed to produce smaller oligomers of cellulose, cellbiose, and/or glucose. The kosmotropic anion facilitates phase separation where the aqueous phase contains very low concentrations of the ionic liquid. | 09-26-2013 |
20130252286 | TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, AND METHOD FOR PRODUCING SAME - To provide a transformant of a yeast of the genus | 09-26-2013 |
20130260423 | Methods of Saccharifying Sugar Cane Trash - The present invention relates to methods of saccharifying the trash (leaf) fraction of sugar cane using enzymes. | 10-03-2013 |
20130266990 | STEPWISE ENZYMATIC HYDROLYSIS PROCESS FOR CONVERTING CELLULOSE TO GLUCOSE - A method for the production of alcohol and other bioproducts hemicelluloses extracted from biomass prior to thermal conversion of the biomass to energy. The process can be integrated with the host plant process to minimize the energy loss from extracting hemicelluloses. Also disclosed is a Stepwise enzymatic break down of cellulose fibers from a pulping operation which is performed with the redeployment of equipment and vessels contained within typical existing pulp and paper manufacturing mills. The preferred feedstock is highly delignified pulp from acid or alkaline pulping process or from bleaching stage. | 10-10-2013 |
20130266991 | METHOD FOR PRODUCING CONCENTRATED AQUEOUS SUGAR SOLUTION - A method produces a concentrated aqueous sugar solution using a cellulose-containing biomass as a raw material, including:
| 10-10-2013 |
20130273611 | XYLANASES ACTIVE DURING PRETREATMENT OF CELLULOSIC BIOMASS - Compositions and methods are provided for treating lignocellulosic material with a xylanase enzyme having xylanase activity. The enzyme is stable and active at increased pHs and temperatures. The present invention therefore provides methods for hydrolyzing lignocellulosic material, especially cellulose and hemicellulose, which are major components of the cell wall of non-woody and woody plants. The methods for hydrolyzing cellulose and hemicellulose can be used on any plant, wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. | 10-17-2013 |
20130273612 | PROCESSING BIOMASS AND PETROLEUM CONTAINING MATERIALS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation. | 10-17-2013 |
20130280764 | METHOD OF IMPROVING THE ACTIVITY OF CELLULASE ENZYME MIXTURES IN THE SACCHARIFICATION (LIGNO)CELLULOSIC MATERIAL - The present invention relates to modified filamentous fungal organisms having improved activity profiles with respect to the conversion of complex carbohydrates into simple sugars from cellulosic materials, including fungal organisms belonging to a genus selected from the group consisting of: | 10-24-2013 |
20130288310 | RECOMBINANT LIGNOCELLULOSE DEGRADATION ENZYMES FOR THE PRODUCTION OF SOLUBLE SUGARS FROM CELLULOSIC BIOMASS - The invention relates to C1 lignocellulose degradation enzyme nucleic acid and protein sequences and expression of recombinant C1 lignocellulose degradation enzymes. The invention provides methods for degrading a cellulosic biomass by contacting the biomass with a recombinant C1 lignocellulose degradation enzyme of the invention. | 10-31-2013 |
20130295619 | ACIDOTHERMUS CELLULOYTICUS XYLANASE - A thermophilic endo-beta-1,4-xylanase derived from | 11-07-2013 |
20130295620 | SACCHAROMYCES CEREVISIAE STRAINS - A method for producing a strain of | 11-07-2013 |
20130302858 | METHODS OF COMBINED BIOPROCESSING AND RELATED MICROORGANISMS, THERMOPHILIC AND/OR ACIDOPHILIC ENZYMES, AND NUCLEIC ACIDS ENCODING SAID ENZYMES - A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from | 11-14-2013 |
20130309727 | METHODS AND SYSTEMS FOR PROCESSING BIOMASS MATERIAL - Embodiments of the present invention provide for efficient and economical production and recovery of ethanol or other volatile organic compounds. One embodiment comprises contacting a solid component of a biomass material with a solution adapted to facilitate saccharification. The solid component is generated by a method comprising: introducing a biomass material to a compartment of a solventless recovery system, wherein the biomass material contains one or more volatile organic compounds; contacting the biomass material with a superheated vapor stream in the compartment to vaporize at least a portion of an initial liquid content in the biomass material; separating a vapor component and a solid component from the heated biomass material; and retaining at least a portion of the gas component for use as part of the superheated vapor stream. In one embodiment, the solid component contacted with the solution is further subjected to enzymatic hydrolysis and/or fermentation. | 11-21-2013 |
20130309728 | PROCESS FOR PRODUCING HEMICELLULOSE SUGARS AND ENERGY FROM BIOMASS - A method for the production of alcohol and other bioproducts hemicelluloses extracted from biomass prior to thermal conversion of the biomass to energy. The process can be integrated with the host plant process to minimize the energy loss from extracting hemicelluloses. Also disclosed is a Stepwise enzymatic break down of cellulose fibers from a pulping operation which is performed with the redeployment of equipment and vessels contained within typical existing pulp and paper manufacturing mills. The preferred feedstock is highly delignified pulp from acid or alkaline pulping process or from bleaching stage. | 11-21-2013 |
20130309729 | MUTANT CELLOBIOHYDROLASE - The invention relates to Mutant cellobiohydrolase, being a mutant of SEQ ID NO:1, having a substitution at position N247(I,F,H,W) of SEQ ID NO: 1, wherein the mutant cellobiohydrolase has at least 50% sequence identity with SEQ ID NO: 1, and wherein the mutant cellobiohydrolase has CBHI activity. | 11-21-2013 |
20130316407 | PROCESS OF PRODUCING A FERMENTATION PRODUCT - The invention relates to a process of producing a fermentation product in the presence of pyridoxamine. | 11-28-2013 |
20130323798 | METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION - A fungal α-amylase is provided from | 12-05-2013 |
20130330781 | Endoglucanases - The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity, which perform very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications. | 12-12-2013 |
20130330782 | LIQUEFACTION BIOMASS PROCESSING WITH HEAT RECOVERY - Described are processes that include the non-enzymatic, hydrolytic liquefaction of lignocellulosic biomass to form digest slurries and heat recovery from such digest slurries. Due to enhanced flow properties of the digest slurries such heat recovery can be efficiently conducted in spiral, plate and frame or other heat exchanger designs, with the recovered heat going to unit operations of the process such as heating incoming pretreatment media for the liquefaction. Processes can also involve additional hydrolytic digestion of some or all of the initial slurry components with enzyme and/or additional heat recovery from the initial slurry by direct contact heat exchange in which a portion of the digest slurry liquids is flashed to vapor and that vapor is condensed onto incoming lignocellulosic biomass to the process. Processes as described can be integrated into ethanol manufacture by fermentation of sugars from the digested compositions. | 12-12-2013 |
20130330783 | CELLULASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - This invention relates to molecular and cellular biology and biochemistry. In one aspect, the invention provides polypeptides having cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or β-glucosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or β-glucosidase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. | 12-12-2013 |
20130330784 | TEMPERATURE-STABLE BETA-PYRANOSIDASE - The invention relates to temperature-stable polypeptides with β-pyranosidase activity. The polypeptide substrates include β-glucopyranosides und β-xylopyranosides. Said polypeptides can be expressed alone or as fusion proteins for example in yeast or bacteria and subsequently purified. The polypeptides according to said invention can be used alone or in a mixture with other enzymes for the degradation of plant raw materials, among others for the enzymatic degradation of biomass containing lignocellulose, in particular hemicellulose and the hemicellulose component xylan. Said enzymes are suitable for use in textile processing, as an additive of detergents, or in the food or feed industry. | 12-12-2013 |
20130330785 | Processes of Producing a Fermentation Product - The invention relates to a process of process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase and/or an amino acid oxidase; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism. | 12-12-2013 |
20130337507 | Xylanases, Nucleic Acids Encoding Them and Methods for Making and Using Them - The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use thereof are also provided. The xylanases have increased activity and stability at increased pH and temperature. | 12-19-2013 |
20130337508 | BETA-GLUCOSIDASE ENHANCED FILAMENTOUS FUNGAL WHOLE CELLULASE COMPOSTIONS AND METHODS OF USE - The present disclosure provides beta-glucosidase enhanced filamentous fungal whole cellulase compositions. Also provided are methods of hydrolyzing a cellulosic material with beta-glucosidase enhanced whole cellulase compositions. The present disclosure further provides methods of decreasing the amount of a whole cellulase required to hydrolyze a cellulosic material by adding an effective amount beta-glucosidase. | 12-19-2013 |
20130337509 | Processes for Enzymatic Refining of Pretreated Cellulosic Material for Saccharification - The present invention relates to processes for processes for enzymatic refining of a pretreated cellulosic material for saccharification. | 12-19-2013 |
20130337510 | Methods and compositions for degrading cellulosic material - The present invention relates to enzyme compositions comprising a polypeptide having cellobiohydrolase II activity, a polypeptide having xylanase activity, and one or more cellulolytic proteins and their use in the degradation or conversion of cellulosic material. | 12-19-2013 |
20130344541 | COMPOSITIONS AND METHODS FOR PRODUCTION OF FERMENTABLE SUGARS - The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures. | 12-26-2013 |
20130344542 | TS-23 Alpha-Amylase Variants With Altered Properties - Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof. | 12-26-2013 |
20130344543 | METHOD FOR PRODUCING SUGAR SOLUTION - A method produces a sugar liquid by adding a filamentous fungus-derived cellulase to a pretreated product of cellulose to obtain a hydrolysate; adding waste molasses to said hydrolysate to obtain a mixed sugar liquid; and subjecting said mixed sugar liquid to solid-liquid separation and filtering the obtained solution component through an ultrafiltration membrane, to recover the filamentous fungus-derived cellulase as a non-permeate and to obtain a sugar liquid as a permeate. | 12-26-2013 |
20130344544 | Amylases, Nucleic Acids Encoding Them and Methods for Making and Using Them - In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars. | 12-26-2013 |
20140004571 | COMPOSITIONS AND METHODS FOR BIOMASS LIQUEFACTION | 01-02-2014 |
20140004572 | A-XYLOSIDASE ENHANCED CONVERSION OF PLANT BIOMASS INTO FERMENTABLE SUGARS | 01-02-2014 |
20140004573 | PROCESSING BIOMASS | 01-02-2014 |
20140004574 | PRODUCTION OF PRODUCTS FROM BIOMASS | 01-02-2014 |
20140011242 | METHOD FOR IMPROVING YIELD OF CELLULOSE CONVERSION PROCESSES - The present teachings provide methods of converting cellulosic materials to soluble sugars. Methods for increasing the yield of glucose from the enzymatic saccharification of cellulosic materials is also provided. The present teachings further provide methods of increasing the yield of cellobiose from the enzymatic saccharification of cellulosic materials. | 01-09-2014 |
20140017732 | TWO-LOOP DILUTE PREPROCESSING AND PRETREATMENT OF CELLULOSIC FEEDSTOCKS - The present application describes a dilute two-loop method for treating biomass in the production of biofuels such as ethanol. The method involves washing the biomass to dissolve sugars that are associated with the biomass, and separating the washed biomass into a solids phase and a liquids phase. The solids phase is pretreated to render the biomass more susceptible to hydrolysis under conditions that do not produce substantial amounts of sugars. The pretreated biomass is separated into a second solids phase and second liquid phase, and the second solids phase is saccharified and fermented. The first and second liquid phases are recycled to dilute the biomass at various stages of the process. | 01-16-2014 |
20140017733 | FUNGAL XYLANASES AND XYLOSIDASES - The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are | 01-16-2014 |
20140017734 | CHIMERIC ENZYMES WITH IMPROVED CELLULASE ACTIVITIES - Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose. | 01-16-2014 |
20140017735 | ENZYMES FOR IMPROVED BIOMASS CONVERSION - Disclosed herein are enzymes and combinations of the enzymes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using enzymes and cocktails of enzymes are also disclosed. | 01-16-2014 |
20140017736 | METHOD FOR PRODUCING SUGAR SOLUTION - A method of producing a sugar liquid includes hydrolyzing with a filamentous fungus-derived cellulase a product obtained by adding a water-soluble inorganic salt(s) to a pretreated product of cellulose to a final concentration of 5 to 35 g/L; and subjecting the hydrolysate to solid-liquid separation and filtering the obtained solution component through an ultrafiltration membrane to recover the filamentous fungus-derived cellulase as a non-permeate and to obtain a sugar liquid as a permeate. | 01-16-2014 |
20140017737 | Cellobiohydrolase variants and polynucleotides encoding same - The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 01-16-2014 |
20140024083 | Alpha-Amylase Mutants - The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca | 01-23-2014 |
20140024084 | MATERIALS AND METHODS FOR CONVERTING BIOMASS TO BIOFUEL - This disclosure provides for materials and methods for converting biomass to biofuels. The materials include a colloid mill with or without cellulase enzymes, and the methods include the use of a colloid mill and optionally cellulose enzymes to pretreat biomass for use in a biomass to biofuel production process. | 01-23-2014 |
20140030767 | Alpha-Amylase Mutants with Altered Properties - The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca | 01-30-2014 |
20140030768 | Processing Biomass Containing Materials - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation. | 01-30-2014 |
20140030769 | FREE ENZYME AND CELLULOSOME PREPARATIONS FOR CELLULOSE HYDROLYSIS - Disclosed herein are combinations of free fungal enzymes and cellulosomes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using the combinations are also disclosed. | 01-30-2014 |
20140030770 | Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 01-30-2014 |
20140038243 | METHODS FOR PRETREATING BIOMASS - A method for treating biomass is provided, which includes, in a reactor, allowing gaseous ammonia to condense on the biomass and react with water present in the biomass to produce pretreated biomass, wherein reactivity of polysaccharides in the biomass is increased during subsequent biological conversion as compared to polysaccharides in biomass which has not been pretreated. A method for treating biomass with a liquid ammonia and recovering the liquid ammonia is also provided. Related systems which include a biochemical or biofuel production facility are disclosed. | 02-06-2014 |
20140038244 | INTEGRATED WOOD PROCESSING AND SUGAR PRODUCTION - Provided is a system for localizing and optimizing harvesting of woody biomass by providing a plurality of portable pretreatment units at harvesting or mill sites. The sugars derived from woody waste products can be transported to plants for processing or processing directly by plants located at mill sites to generate biofuels and other chemicals. | 02-06-2014 |
20140045224 | PROCESS FOR PRODUCING SACCHARIFIED SOLUTION - A process for producing a saccharified solution in an improved yield when filtering the mixture obtained by saccharifying lignocellulosic biomass is provided. A mixture containing a saccharified solution and an undecomposed biomass residue by treating a substrate biomass using a saccharification enzyme is obtained. The mixture is filtered to obtain the saccharified solution as a filtrate. Before filtering the mixture, 95% by mass or more of the particles, based on the total amount of particles contained in a centrifuged supernatant of the mixture, is adjusted to have a particle size ranging from 1 to 30 μm. | 02-13-2014 |
20140045225 | Proteins for the Treatment of Cellulosic Material - The present invention discloses novel polypeptides and enzyme preparations containing them, which enhance the efficiency of the cellulosic degradation even at elevated temperatures. The polypeptides are produced by recombinant technology, and means for their production are described. The novel polypeptides are useful in processing biomass, and in biofuel, starch, textile, detergent, pulp and paper, food, feed or beverage industries. They may also be used e.g. in cleaning the interior of a dishwashing machine or for biofinishing or biostoning. The novel polypeptides are also useful in animal feed. | 02-13-2014 |
20140045226 | PROCESS FOR TREATING LIGNOCELLULOSIC BIOMASS - A process for treating lignocellulosic biomass is described. The process includes treating particulate biomass with alkali to partially remove hemicellulose sheath on cellulose microfibrils of the biomass; and carrying out an oxidation reaction on the alkali treated biomass to form a treated biomass having a disrupted cellulose crystalline structure. The treated biomass may be further processed to form free sugars and lignin and the free sugars can be used to produce bio-ethanol or used as sugar source in fermentation processes. | 02-13-2014 |
20140045227 | EFFICIENT LIGNOCELLULOSE HYDROLYSIS WITH INTEGRATED ENZYME PRODUCTION - The present invention provides a process for degradation of lignocellulosic biomass, which has optionally been pre-treated. It is based on the finding that hydrolysis efficiency of the biomass is enhanced in the presence of a mechanically or chemically treated microorganism capable of producing the respective hydrolytic enzymes. The invention therefore provides a process for degradation of lignocellulosic biomass with integrated produced enzyme cocktails. The invention also provides a process wherein the part of the optionally pre-treated lignocellulosic biomass is incorporated into the final growth medium of the fungus. | 02-13-2014 |
20140051128 | Endoglucanases for Treatment of Cellulosic Material - The present invention relates to production of fermentable sugars from lignocellulosic material by enzymatic conversion. The fermentable sugars are useful e.g. in the production of bioethanol. Novel polypeptides having endoglucanase activity, polynucleotides encoding them and vectors and host cells containing the polynucleotides are disclosed. A method for treating cellulosic material with the novel endoglucanase as well as uses of the enzymes and enzyme preparations and a method of preparing them are described. | 02-20-2014 |
20140051129 | POTENTIATION OF ENZYMATIC SACCHARIFICATION - The present disclosure provides methods of potentiating the activity of an enzyme cocktail by the addition of one or more enzymes. In some embodiments, a sub-maximum or sub-optimal dose of the cocktail may be used in combination with the enzymes. In some embodiments, the enzyme or enzymes are expressed in planta. | 02-20-2014 |
20140051130 | Compositions for saccharification of cellulosic material - The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof. | 02-20-2014 |
20140057324 | Variants of An Alpha-Amylase With Improved Production Levels in Fermentation Processes - Variants of | 02-27-2014 |
20140065677 | Novel Glycoside Hydrolases from Thermophlic Fungi - The present invention relates to isolated polypeptides having cellulolytic activity or hemicellulolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 03-06-2014 |
20140073016 | Treatment - The present invention relates to a method for treating a lignocellulose biomass in order to dissolve the lignin therein, while the cellulose does not dissolve. The cellulose pulp obtained can be used to produce glucose. In addition the lignin can be isolated for subsequent use in the renewable chemical industry as a source for aromatic platform chemicals. | 03-13-2014 |
20140073017 | CELLULASE COMPOSITIONS AND METHODS OF USING THE SAME FOR IMPROVED CONVERSION OF LIGNOCELLULOSIC BIOMASS INTO FERMENTABLE SUGARS - The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having β-glucosidase activity, methods for hydrolyzing biomass material, and methods for improving the stability and saccharification efficacy of a composition comprising such β-glucosidase polypeptides and/or activity. | 03-13-2014 |
20140080182 | Cellulose Binding Domain Variants and Polynucleotides Encoding Same - The present invention relates to cellobiohydrolase variants and cellulose binding domain variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 03-20-2014 |
20140080183 | HYBRID SEPARATION - This disclosure describes techniques for using a single feedstock of barley to produce a fermented product and a method for filtering a large-particles stream from a liquid stream containing small particles of a process stream using a series of mechanical separation devices to increase yield. | 03-20-2014 |
20140087432 | METHODS FOR CONDITIONING PRETREATED BIOMASS - Methods for producing ethanol from cellulosic biomass and, in particular, methods for conditioning pretreated biomass are disclosed. In some embodiments, pretreated biomass is contacted with a cooling fluid in a flash vessel to cool the biomass. The amount of alkaline solution contacted with the biomass may be based on the pH of partially hydrolyzed pretreated biomass in a liquefaction bioreactor. | 03-27-2014 |
20140087433 | Processing Biomass - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation. | 03-27-2014 |
20140093917 | METHODS FOR CONVERTING LIGNOCELLULOSIC MATERIAL TO USEFUL PRODUCTS - The present invention provides compositions and methods for the pretreatment of lignocellulosic material. The present invention further provides for pretreated lignocellulosic material that can be used to produce useful products, such as fermentable sugars. | 04-03-2014 |
20140093918 | METHODS FOR CONVERTING LIGNOCELLULOSIC MATERIALS TO USEFUL PRODUCTS - The present invention provides compositions and methods for the pretreatment of lignocellulosic material. The present invention further provides for pretreated lignocellulosic material that can be used to produce useful products, such as fermentable sugars. | 04-03-2014 |
20140093919 | Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 04-03-2014 |
20140099677 | ALTERING ENZYME BALANCE THROUGH FERMENTATION CONDITIONS - This present disclosure relates to methods for improved production of proteins from a cell culture, particularly to culture components and conditions that can preferentially increase the expression of proteins produced from genes under the control of xylanase gene promoter sequences. The improved methods can be used for the production of enzyme compositions with enhanced xylanase and hem icellulolytic activity. | 04-10-2014 |
20140099678 | Fungal Endoglucanases, Their Production and Use - Novel fungal endoglucanases Cel5 and Cel12 are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry, or in hydrolysis of lignocellulosic material for, e.g. bioethanol production. | 04-10-2014 |
20140106407 | REDUCING NON-SPECIFIC ENZYME BINDING TO ENHANCE LIGNOCELLULOSE CONVERSION - A system is provided for reducing non-specific binding of an enzyme to lignin to enhance an enzymatic processing of a lignocellulosic material. The enhancements provide economic and process advantages to any process that converts a lignocellulosic biomass into a product using an enzyme. Systems are provided comprising a reaction vessel; a lignocellulosic feedstock comprising a component selected from the group consisting of a hardwood, a softwood, or a non-wood material; an enzyme component including a cellulase, a hemicellulase, or a combination thereof; and, water. The reaction vessel can contain a combination of the lignocellulosic feedstock, the water, and the enzyme component at a pH ranging from about 5.2 to about 6.2; and, the lignocellulosic feedstock can be saccharified in the reaction vessel. Moreover, the systems can include a lignosulfonate, with or without a pH of about 5.2 to about 6.2, to also reduce non-specific binding and enhance enzymatic activity. | 04-17-2014 |
20140106408 | GLYCOSYL HYDROLASE ENZYMES AND USES THEREOF FOR BIOMASS HYDROLYSIS - The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase (GH) family 61/endoglucanase activity and/or a β-glucosidase polypeptide, methods for hydrolyzing biomass material, and methods for using such compositions. | 04-17-2014 |
20140106409 | CHIMERIC ALPHA-AMYLASE VARIANTS - Chimeric alpha-amylases having the characteristics of high thermostability and good performance in starch degradation, especially high-temperature liquefaction processes, are provided. The alpha-amylases are chimeras of AmyL and AmyS enzymes, and are useful in starch degradation processes. Methods of making the chimeric enzymes, and methods of using the chimeric alpha-amylases for liquefaction, cleaning starch residue from a surface, and treating woven material to remove coatings. Kits for practicing the methods are provided. Polynucleotides encoding the chimeric amylases, vectors, and expression hosts also are provided. | 04-17-2014 |
20140106410 | Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 04-17-2014 |
20140113334 | METHOD AND APPARATUS FOR MIXING A LIGNOCELLULOSIC MATERIAL WITH ENZYMES - A method to treat a lignocellulosic material including: pretreating the lignocellulosic material to produce a slurry; adding an alkali material to the slurry in a first conveyor; adding a first portion of an enzyme solution to the slurry while the slurry is in, entering or passing through the discharge of a second conveyor; transferring the slurry with the enzyme solution from the second conveyor slurry to a mixer; mixing the slurry with a second portion of the enzyme solution, and transferring the slurry with the enzyme solution mixed with the first and second portions of the enzyme solution to a first reactor vessel for enzymatic hydrolysis, wherein the viscosity of the slurry is reduced in the first reactor vessel. | 04-24-2014 |
20140113335 | Improved Cellulase Variants - This invention provides novel variant cellulolytic enzymes having improved activity and/or stability. In certain embodiments the variant cellulotyic enzymes comprise a glycoside hydrolase with or comprising a substitution at one or more positions corresponding to one or more of residues F64, A226, and/or E246 in | 04-24-2014 |
20140113336 | Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 04-24-2014 |
20140120586 | Esterases Useful in the Treatment of Cellulosic and Lignocellulosic Material - The present invention discloses novel polypeptides and enzyme preparations containing them, which improve the efficiency of cellulose and lignocellulose degradation even at elevated temperatures. The polypeptides can be produced using conventional recombinant DNA technologies. The related polynucleotides, vectors and host cells are also disclosed. The polypeptides and the enzyme preparations containing them are particularly useful in improving the efficiency of cellulose and lignocellulose degradation, in improving the quality of animal feed, in machine dishwashing applications, in detergent compositions, in pulp and paper, textile, food, baking or beverage industry. | 05-01-2014 |
20140127753 | ENZYMES FOR STARCH PROCESSING - The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides. | 05-08-2014 |
20140127754 | METHODS AND COMPOSITIONS FOR DEGRADATION OF LIGNOCELLULOSIC MATERIAL - Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. | 05-08-2014 |
20140127755 | PROCESS FOR TREATING BIOMASS TO DERIVATIZE POLYSACCHARIDES CONTAINED THEREIN TO INCREASE THEIR ACCESSIBILITY TO HYDROLYSIS AND SUBSEQUENT FERMENTATION - A process is described for producing fermentable sugars derivable from biomass that contains polysaccharide, such as cellulose, made increasingly accessible as a substrate for enzymatic degradation or other methods of depolymerization. These fermentable sugars are subsequently able to be fermented to produce various target chemicals, such as alcohols, aldehydes, ketones or acids. | 05-08-2014 |
20140127756 | HIGH SOLIDS ENZYME REACTOR MIXER WITH VERTICAL PADDLE AND METHOD - A reactor vessel including: a mixing chamber having a vertical length, an upper inlet, and a lower outlet; and a vertically oriented paddle within the mixing chamber and having a cross-sectional shape of a hydrofoil, wherein the paddle moves with respect to the mixing chamber. | 05-08-2014 |
20140127757 | HETEROLOGOUS ALPHA AMYLASE EXPRESSION IN ASPERGILLUS - The present invention relates to the co-expression and production of a heterologous alpha amylase and an endogenous glucoamylase in an | 05-08-2014 |
20140127758 | Methods For Enhancing The Degradation Or Conversion Of Cellulosic Material - The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material under high temperature conditions. | 05-08-2014 |
20140127759 | MUTANT B-GLUCOSIDASE, ENZYME COMPOSITION FOR DECOMPOSING BIOMASS, AND METHOD OF PRODUCING SUGAR SOLUTION - A β-glucosidase exhibits high activity in the presence of biomass and has high thermal stability compared to conventional enzymes. The β-glucosidase includes substitutions and/or deletions of amino acids, at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent β-glucosidase with other amino acids and exhibits a decomposition activity. | 05-08-2014 |
20140127760 | Method of Producing Corn Starch by Enzymatic Process - A method of producing corn starch by enzymatic process involving: soaking the corn; crushing the corn, separating and washing embryo; fine grinding; washing and drying fiber; separating and drying protein; washing, dewatering and drying the starch. An enzyme preparation is added before the step of washing, dewatering and drying the starch; the enzyme preparation is cellulose, or xylanase, or combination of the cellulose and the xylanase; and addition of the enzyme preparation is from 0.001% to 0.08% by weight of the corn. Based on the technology of traditional wet process, the method of the present invention comprises a step of adding enzyme preparation in the process of separating the corn, which improves the effect and the efficiency of mechanical separation, and further improves the purity and yield of the substance separated while also reducing the energy consumption. | 05-08-2014 |
20140134677 | METHOD FOR REDUCING VISCOSITY IN SACCHARIFICATION PROCESS - The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity, methods for hydrolyzing biomass material, and methods for reducing viscosity of biomass mixture using a composition comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity. | 05-15-2014 |
20140134678 | METHOD OF HYDROLYZING CELLULOSE SLURRY USING CELLULASE AND FLOCCULENT (AS AMENDED) - A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product from a pre-treated cellulosic feedstock is provided. The process comprises introducing an aqueous slurry of the pre-treated cellulosic feedstock at the bottom of a hydrolysis reactor. Axial dispersion in the reactor is limited by avoiding mixing and maintaining an average slurry flow velocity of about 0.1 to about 20 feet per hour, such that the undissolved solids flow upward at a rate slower than that of the liquid. Cellulase enzymes are added to the aqueous slurry before or during the step of introducing. An aqueous stream comprising hydrolysis product and unhydrolyzed solids is removed from the hydrolysis reactor. Also provided are enzyme compositions which comprise cellulase enzymes and flocculents for use in the process. In addition, a kit comprising cellulase enzymes and flocculent is provided. | 05-15-2014 |
20140141471 | Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins - The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material. | 05-22-2014 |
20140141472 | Methods For Degrading or Converting Plant Cell Wall Polysaccharides - The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation. | 05-22-2014 |
20140147894 | VARIANT CBH I POLYPEPTIDES WITH REDUCED PRODUCT INHIBITION - The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides. | 05-29-2014 |
20140147895 | Processes for Pretreating Cellulosic Material and Improving Hydrolysis Thereof - The present invention relates to processes for pre-treating cellulosic material and processes for improving hydrolysis thereof. In particular, cellulosic material such as woody biomass is contacted with one or more enzymes in a re-pulping step. The cellulosic material is then contacted with one or more enzymes to improve hydrolysis of the cellulosic material. The hydrolysis is also enzymatically enhanced by use of an amylase and/or mannanase. | 05-29-2014 |
20140154749 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids. | 06-05-2014 |
20140154750 | STRAIN OF BACILLUS SUBTILIS AND APPLICATIONS THEREOF - The present invention is directed to a strain of | 06-05-2014 |
20140154751 | ENHANCED FERMENTATION OF CELLODEXTRINS AND BETA-D-GLUCOSE - The present disclosure provides compositions and methods for the fermentation of cellodextrins and β-D-glucose. Host cells and recombinant polypeptides having glucose mutarotase activity are provided. Additionally, methods for improving cell growth, production of chemicals, and consumption of cellodextrins and β-D-glucose during fermentation of mixtures containing cellodextrins and β-D-glucose are provided. | 06-05-2014 |
20140154752 | GLYCOSIDE HYDROLASES HAVING MULTIPLE HYDROLASE ACTIVITIES - Glycoside hydrolases having at least two different hydrolytic activities are provided. In one embodiment, an isolated recombinant hydrolase having at least two activities selected from a group including asparagine derivatives, glutamine derivatives, and histidine derivatives is provided. Further, a method of generating free sugars from a mixture comprising asparagine derivatives, glutamine derivatives, and histidine derivatives is provided. | 06-05-2014 |
20140162323 | METHODS OF COMBINED BIOPROCESSING AND RELATED MICROORGANISMS, THERMOPHILIC AND/OR ACIDOPHILIC ENZYMES, AND NUCLEIC ACIDS ENCODING SAID ENZYMES - A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from | 06-12-2014 |
20140170713 | BIOMASS FRACTIONATION PROCESSES, APPARATUS, AND PRODUCTS PRODUCED THEREFROM - Aspects of the AVAP® and Green Power+® technologies may be integrated, as disclosed herein. The present invention, in some variations, couples a first step of steam or hot-water extraction of biomass, with fractionation of the resulting solids using sulfur dioxide (or other acid), an alcohol (or other solvent), and water. In other variations, a first step of fractionation with sulfur dioxide (or other acid), an alcohol (or other solvent), and water is followed by treatment with steam or hot water to reduce hemicellulose content of the final solids. Some embodiments provide cellulose materials with low lignin and low hemicellulose content. Such cellulose materials are useful for making glucose by hydrolysis, as a pulp product, or as purified cellulose for making cellulose derivatives. | 06-19-2014 |
20140178944 | Preservation of Biomass for Pretreatment - Provided are methods and compositions directed to increasing the rate and/or yield of sugar extraction processes using feedstocks. Also provided are methods and compositions for decreasing the yield of one or more undesirable products during pretreatment. | 06-26-2014 |
20140178945 | PROCESS FOR CONVERSION OF GRANULAR STARCH TO ETHANOL - The present invention concerns a method of producing glucose from a granular starch substrate comprising, contacting a slurry comprising granular starch obtained from plant material with an alpha-amylase at a temperature below the starch gelatinization temperature of the granular starch to produce oligosaccharides and hydrolyzing the oligosaccharides to produce a mash comprising at least 20% glucose and further comprising fermenting the mash to obtain ethanol. | 06-26-2014 |
20140178946 | MATERIALS AND METHODS FOR CONVERTING BIOMASS TO BIOFUEL - This disclosure provides for materials and methods for converting biomass to biofuels. The materials include a colloid mill with or without cellulase enzymes, and the methods include the use of a colloid mill and optionally cellulose enzymes to pretreat biomass for use in a biomass to biofuel production process. | 06-26-2014 |
20140178947 | MUTANT ENDOGLUCANASE - Endoglucanase characterized by a decreased degree of activity inhibition by a lignin-derived aromatic compound, and prepared by substituting tryptophan at position 273 in the amino acid sequence of wild-type thermophilic bacterium-derived endoglucanase with an amino acid other than aromatic amino acids. | 06-26-2014 |
20140186896 | USE OF GLYCOSIDE HYDROLASE 61 FAMILY PROTEINS IN PROCESSING OF CELLULOSE - The invention provides recombinant GH61 proteins obtained from | 07-03-2014 |
20140186897 | TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, METHOD FOR PRODUCING SAME, METHOD FOR PRODUCING -GLUCOSIDASE, AND CELLULOSE DEGRADATION METHOD - To provide a transformant of a yeast of the genus | 07-03-2014 |
20140186898 | PROCESSES FOR FRACTIONATING WHOLE PLANTS TO PRODUCE FERMENTABLE SUGARS AND CO-PRODUCTS - The invention provides processes for producing fermentable sugars from whole biomass that includes cellulose, hemicellulose, lignin, and non-lignocellulosic sugars. Some variations fractionate the whole feedstock in the presence of sulfur dioxide, a solvent for lignin, and water, to produce a liquor containing hemicellulose, cellulose-rich solids, lignin, and the non-lignocellulosic sugars. After removing the cellulose-rich solids from the liquor, the hemicellulose is hydrolyzed to hemicellulosic monomers; the cellulose-rich solids are hydrolyzed to glucose; and the hemicellulosic monomers, the glucose, and the non-lignocellulosic sugars are all recovered (separately or in combination) as fermentable sugars. The whole biomass feedstock may be selected from sugarcane, energy cane, corn, wheat, rice, sugar beets, energy beets, etc. Typical non-lignocellulosic sugars are sucrose or starch, which may be converted to monomer sugars during initial fractionation. The invention provides a convenient biorefining system, avoiding expensive logistics associated with separating agricultural residues at harvest or following transportation. | 07-03-2014 |
20140186899 | BIOMASS FRACTIONATION PROCESSES EMPLOYING SULFUR DIOXIDE - The present invention provides a process for fractionating lignocellulosic biomass, comprising: contacting biomass with SO | 07-03-2014 |
20140206043 | DISPERSING FEEDSTOCKS AND PROCESSING MATERIALS - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is dispersed in a liquid medium and then saccharified. | 07-24-2014 |
20140206044 | PROCESSING MATERIALS - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is saccharified in a vessel by operation of a jet mixer, the vessel also containing a liquid medium and a saccharifying agent. | 07-24-2014 |
20140206045 | Thermostable Alpha-Amylases - The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of:
| 07-24-2014 |
20140206046 | Methods of Hydrolyzing and Fermenting Cellulosic Material - Described herein are improved methods of degrading or converting cellulosic material into fermentable sugars using dithionite. Also described are improved methods of fermentation in the presence of dithionite. | 07-24-2014 |
20140212930 | DIVERSIFICATION OF HUMAN MILK OLIGOSACCHARIDES (HMOs) OR PRECURSORS THEREOF - A method of diversification of human milk oligosaccharides (HMOs) or precursors thereof, compounds obtainable by the method, and uses and compositions involving such compounds. The method comprises a) providing at least one compound or a mixture of the compounds selected from the group consisting of: optionally sialylated and/or fucosylated lactose derivatives of general formula 2 and salts thereof; b) adding at least one enzyme comprising a transglycosidase activity to the at least one compound or a mixture of compounds provided according to step a); and c) incubating the mixture obtained according to step b). | 07-31-2014 |
20140220640 | METHOD FOR MANUFACTURING DETOXIFICATED LIGNOCELLULOSIC BIOMASS HYDROLYSATE WITH DECREASED OR ELIMINATED TOXICITY AND METHOD FOR MANUFACTURING ORGANIC OR AND BIOFUEL USING THE SAME - Disclosed is a method for detoxifying a lignocellulosic biomass hydrolysate, including: preparing a hydrolysate by pretreating a lignocellulosic biomass by hydrolysis; and decreasing or removing toxicity by adding a surfactant to the hydrolysate. The detoxifying method according to the present disclosure may effectively remove toxicity of compounds derived from lignin that inhibit the growth of and fermentation by microorganisms during the pretreatment of lignocellulosic biomass. Further, production efficiency can be improved since loss of sugar can be avoided during the detoxification and additional cost can be minimized. | 08-07-2014 |
20140220641 | TRANSCRIPTION FACTORS FOR CELLULOSIC ENZYME PRODUCTION - Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell. Advantageously, mis-expression of the transcription factor clr-2 in a filamentous fungal cell cultured in the absence of cellulose or cellobiose results in increased secretion of cellulases. The disclosure relates inter alia to a method of degrading cellulose-containing material, to a method of increasing the production of one or more cellulases from a fungal cell and to a method of reducing the viscosity of a pretreated biomass material, by contacting pretreated biomass material with a fungal host cell containing at least one recombinant nucleic acid encoding clr-2 or a related transcription factor. | 08-07-2014 |
20140220642 | Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same - The present invention provides isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 08-07-2014 |
20140234911 | SYSTEMS AND METHODS FOR HYDROLYSIS OF BIOMASS - Systems and methods are disclosed for treating lignocellulosic biomass to be supplied to a fermentation system for production of a fermentation product. The systems and methods comprise pre-treating the biomass into pre-treated biomass and separating the pre-treated biomass into a liquid component comprising sugars and a solids component comprising cellulose and lignin. The systems and methods also comprise treating the solids component of the pre-treated biomass into a treated component. The biomass comprises lignocellulosic material. Treating the solids component comprises application of an enzyme formulation and makeup water to form a slurry. The enzyme formulation comprises a cellulase enzyme. The makeup water includes a clarified thin stillage composition and/or an anaerobic membrane digester effluent composition. | 08-21-2014 |
20140234912 | METHOD FOR GENERATING HUMAN MILK OLIGOSACCHARIDES (HMOs) OR PRECURSORS THEREOF - A method for generating human milk oligosaccharides (HMOs) or precursors thereof, compounds obtainable by the method, and uses and compositions involving such compounds. The method comprising the steps of a) providing at least one donor selected from the group of compounds of any of formulae 5 to 10, b) providing at least one acceptor from a group of lactose, LNT, LNnT and derivatives thereof, c) providing at least one enzyme comprising a transglycosidase activity and/or a glycosynthase activity, d) preparing a mixture of the at least one donor, at least one acceptor and at least one enzyme provided in steps a), b) and c); and e) incubating the mixture prepared according to step d). | 08-21-2014 |
20140234913 | PROCESS FOR TREATING A SUBSTRATE WITH AN ENZYME - In a process for hydrolyzing plant material in aqueous solution or suspension with an enzyme, the enzyme is delivered in solid form (e.g., as a spray-dried powder) in closed containers (such as paper bags or cardboard boxes), which are added directly in the process (i.e., addition of whole boxes/bags). The invention is particularly amenable to the production of first or second-generation bioethanol. | 08-21-2014 |
20140234914 | Aspergillus Fumigatus Cellulolytic Enzyme Compositions and Uses Thereof - The present invention relates to recombinant | 08-21-2014 |
20140234915 | Cellulolytic Enzyme Compositions and Uses Thereof - The present invention relates enzyme composition comprising a cellulolytic preparation and an acetylxylan esterase (AXE); and the used of cellulolytic enzyme compositions for hydrolyzing acetylated cellulosic material. Finally the invention also relates to processes of producing fermentation products from acetylated cellulosic materials using a cellulolytic enzyme composition of the invention. | 08-21-2014 |
20140248670 | Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins - The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material. | 09-04-2014 |
20140248671 | METHODS OF INCREASING SECRETION OF POLYPEPTIDES HAVING BIOLOGICAL ACTIVITY - The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium. | 09-04-2014 |
20140255998 | Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 09-11-2014 |
20140255999 | SIMPLE SUGAR STARVED LIGNOCELLULOSIC BIOMASS ENZYME PRODUCTION - This specification discloses a process for producing at least one enzyme from a host cell for the hydrolysis of a first pre-treated ligno-cellulosic biomass under simple sugar starved conditions wherein the cultivation environment has very little, preferable no, simple sugars added other than those present in a ligno-cellulosic biomass used to feed and grow the host cell. The cultivation environment is substantially void of fermentation stimulators and inducers of enzyme production. Preferably, the cultivation environment has a high dry matter content of the pre-treated ligno-cellulosic biomass. | 09-11-2014 |
20140256000 | POLYPEPTIDES HAVING CELLULOLYTIC ENHANCING ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention provides isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains. | 09-11-2014 |
20140273104 | PRETREATMENT AND FRACTIONATION OF LIGNOCELLULOSIC BIOMASS - Method and apparatus for enhanced production of sugars and lignin via fractionation of lignocellulosic biomass through sequential ionic liquid pretreatment and mild alkaline treatment. The resulting biomass is easily fractionated and amenable to efficient and rapid hydrolysis and catalytic conversion to valuable products with high recovery of the enzymes used in the hydrolysis. | 09-18-2014 |
20140273105 | GRADIENT PRETREATMENT OF LIGNOCELLULOSIC BIOMASS - Lignocellulosic biomass pretreated with ammonia where the concentration of the biomass in the reaction mixture is reduced over time was found to produce more sugars following saccharification, as compared to equivalent biomass pretreated at constant concentration. The concentration of biomass is a solids concentration, which is the percent of dry biomass relative to the total pretreatment reaction mixture on a weight to weight basis. | 09-18-2014 |
20140273106 | EFFICIENT PROCESS FOR PRODUCING SACCHARIDES AND ETHANOL FROM A BIOMASS FEEDSTOCK - Described herein is a process for producing saccharides and ethanol from biomass feedstock that includes (a) producing an enzyme composition by culturing a fungal strain(s) in the presence of a lignocellulosic medium, (b) using the enzyme composition to saccharify the biomass feedstock, and (c) fermenting the saccharified biomass feedstock to produce ethanol. The process is scalable and, in certain aspects, is capable of being deployed on farms, thereby allowing local production of saccharides and ethanol and resulting in a reduction of energy and other costs for farm operators. Optional steps to improve the biomass-to-fuel conversion efficiency are also contemplated, as are uses for byproducts of the process described herein. | 09-18-2014 |
20140273107 | HYDROLYSIS OF CELLULOSIC FINES IN PRIMARY CLARIFIED SLUDGE OF PAPER MILLS AND THE ADDITION OF A SURFACTANT TO INCREASE THE YIELD - A method for processing a stream of cellulosic fines containing inorganic particles, to increase a hydrolysis yield of polysaccharide degradation enzymes, such fines in a waste stream from a recycled packaging paper mill to produce a stream of fermentable sugars, comprising treating the fines with a surfactant which selectively binds to the inorganic particles and which reduces binding to the inorganic particles by the polysaccharide degradation enzymes, and degrading polysaccharides in the waste stream with the polysaccharide degradation enzymes. | 09-18-2014 |
20140273108 | PROCESS AND METHOD FOR IMPROVING THE ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC BIOMASS BY ADDITION OF HYDROTHERMALLY TREATED STILLAGE - A method of producing hydrolysate from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing and converting complex carbohydrates in the cellulosic material. The hydrolysate produced by the method. Ethanol, organic acids, and organism metabolites produced by the method. Biomass produced by the method. A method of increasing sugar production rate and yield of sugars from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing complex carbohydrates in the cellulosic material and forming sugars. | 09-18-2014 |
20140287469 | FILTRATION - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can be useful for separating solids from liquids of saccharified biomass material slurries. | 09-25-2014 |
20140287470 | PROCESSING BIOMASS AND ENERGY - Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels, heat and energy. | 09-25-2014 |
20140295504 | FUNGAL CELLS AND FERMENTATION PROCESSES - The present invention provides an isolated fungal cell that is capable of producing one or more biomass-degrading enzymes and that exhibits increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. Also provided is a fermentation processes for producing one or more biomass-degrading enzymes comprising a fungal cells exhibiting increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. The biomass-degrading enzymes produced by the isolate fungal cell and fermentation processes of the present invention may be used in a process to produce soluble sugars from biomass. | 10-02-2014 |
20140302566 | ENHANCED ETHANOL FERMENTATION USING BIODIGESTATE - Methods and systems for enhancing ethanol production using a suspending fluid are described. The suspending fluid includes organic material that has at least partially been anaerobically digested and anaerobic microorganisms, and is substantially free of non-anaerobic microorganisms. Also described are methods and systems for hydrolyzing a feedstock for fermentation that include hydrolyzing a feedstock suspension. The feedstock suspension can include feedstock that includes complex sugars, and a suspending fluid, wherein the suspending fluid includes organic material that has at least partially been anaerobically digested and anaerobic microorganisms, and is substantially free of non-anaerobic microorganisms. | 10-09-2014 |
20140308712 | METHOD FOR PRODUCING CONCENTRATED AQUEOUS SUGAR SOLUTION - A method produces a concentrated aqueous sugar solution using a cellulose-containing biomass as a raw material, including:
| 10-16-2014 |
20140308713 | ENDOGLUCANASE HAVING ENHANCED THERMOSTABILITY AND ACTIVITY - A variant Cel5a endoglucanase has increased thermostability, increased enzymatic activity and/or increased expression in a host, relative to wild type Cel5a. The improved variant Cel5a endoglucanase may be used to hydrolyze more cellulose at a higher temperature for a more efficient and cost-effective production of biofuels as compared to wild type Cel5a. | 10-16-2014 |
20140315258 | METHODS FOR CONVERTING CELLULOSIC WASTE TO BIOPRODUCTS - The present invention provides processes for converting cellulosic waste, such as municipal solid waste, to bioproducts such as monosaccharides and fermentation products. | 10-23-2014 |
20140315259 | CELLULOSE CO-FEED FOR DRY MILL CORN ETHANOL OPERATIONS - The present application provide methods for producing ethanol from a biomass. The methods combine sugars produced from a feedstock containing starch with sugars produced from a cellulosic biomass. The methods allow increased amounts of ethanol to be produced from a given solids concentration in the fermenters. The methods also encompass filtering the liquefied feedstock mash through a filter comprising biomass fibers. The biomass filter produces a post-filtered mash stream comprising a high concentration of sugars and a low concentration of non-fermentable solids. The methods provide numerous advantages described herein. | 10-23-2014 |
20140315260 | NOVEL CELLULOSE AND LIGNO-CELLULOSE ACTIVE PROTEINS - Methods to digest carbohydrates, especially lignocelluloses and hemicelluloses, using fungal proteins previously not recognized as having this activity are described. | 10-23-2014 |
20140322762 | Treatment of Cellulosic Material and Enzymes Useful Therein - The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations. | 10-30-2014 |
20140322763 | C1-C2 Organic Acid Treatment of Lignocellulosic Biomass to Produce Acylated Cellulose Pulp, Hemicellulose, Lignin and Sugars and Fermentation of the Sugars - A process for production of C5 and C6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a C | 10-30-2014 |
20140322764 | MUTANT XYLANASE, MANUFACTURING METHOD AND USE THEREFOR, AND METHOD FOR MANUFACTURING SACCHARIFIED LIGNOCELLULOSE - What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50° C. for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue. | 10-30-2014 |
20140322765 | Enhanced Processive Cellulases - Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials. | 10-30-2014 |
20140329284 | Chimeric Polypeptides Having Beta-Glucosidase Activity and Polynucleotides Encoding Same - The present invention relates to chimeric polypeptides having beta-glucosidase activity. The present invention also relates to polynucleotides encoding the chimeric polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the chimeric polypeptides | 11-06-2014 |
20140335570 | Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 11-13-2014 |
20140335571 | LIGNIN-DEGRADING METHODS - Disclosed herein are methods that involve degrading materials that include lignin. In one aspect, the method generally includes contacting a lignin sample with a lignin depolymerase and contacting the lignin sample with an auxiliary compound. In another aspect, the method generally includes contacting an auxiliary compound with a sample that includes uncleaved lignin macromolecules and at least partially cleaved lignin components in intermolecular association with other lignin components and/or at least a portion of the uncleaved lignin macromolecules. In both aspects, the auxiliary compound dissociates at least partially cleaved lignin components from one another and/or the uncleaved lignin macromolecules. | 11-13-2014 |
20140342410 | Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 11-20-2014 |
20140356913 | Enzymes Manufactured in Transgenic Soybean for Plant Biomass Engineering and Organopollutant Bioremediation - A strategy for eliminating or greatly reducing the need for physical/chemical treatments or the use of whole microbes for lignocellulosic biomass and organopollutant degradation is disclosed. The soybean is a practical, cost-efficient and sustainable bioreactor for the production of lignin-degrading and cellulose-degrading enzymes. The use of soybean as a transgenic overexpression platform provides advantages that no other industrial scale enzyme expression system can match. Availability of a battery of related plant biomass degrading enzymes in separate transgenic soybean lines provides unprecedented flexibility in industrial and bioremediation processes. Depending upon the particular application, selected soybean-derived powdered enzyme formulations can be used, and their sequential addition can be orchestrated. Manufacturing enzymes using transgenic soybeans wherein these enzymes are capable of lignocellulose and organopollutant degradation into useful or nontoxic products will dramatically change biomass engineering schemes and environmental remediation practices. This technology has a sum of advantages that other protein expression system cannot duplicate, including the manufacturing of individual enzymes in a cost-effective manner that allows flexibility in cocktail composition, ease of application, and long term storage in the absence of a cold chain. | 12-04-2014 |
20140356914 | ENDOGLUCANASE 1B - The present invention provides endoglucanase 1b (EG1b) suitable for use in saccharification reactions. | 12-04-2014 |
20140363855 | PROCESSES FOR INCREASING BIOALCOHOL YIELD FROM BIOMASS - A process for increasing alcohol yield from biomass (the form or agro- or forest residue, grains, hops, etc.), involving multiple hydrodynamic cavitation treatments of biomass filtrate—both before and after fermentation. Carbohydrates extracted from biomass are subjected to a first cavitation treatment to promote additional conversion into carbohydrates. The carbohydrates are then combined with bacterial species and nutrients, and allowed to ferment. The fermentation product is subjected to a second hydrodynamic cavitation treatment to promote further conversion of carbohydrates into bioalcohol. After distillation, the bioalcohol is subjected to a second hydrodynamic cavitation treatment to increase its purity. | 12-11-2014 |
20140370551 | PRODUCTION OF SUGARS AND CO-PRODUCTS FROM CELLULOSIC WASTE STREAMS - This disclosure provides a business method and system for generating sugars and recycling a non-biomass component from a waste stream. In some embodiments, a waste stream comprising cellulose and a non-biomass component is saccharified to produce glucose, followed by recovery of the glucose and non-biomass component, which may be recycled to another site associated with production of a cellulose-containing product that contains the non-biomass component. In certain scenarios, the waste stream is generated at a first location, cellulose pretreatment (if desired) and hydrolysis are conducted at a second location, and the non-biomass component is recycled to the first location or a third location. The non-biomass component may include metals, metal oxides, salts, organic compounds, inorganic compounds, oligomers, or polymers, for example. | 12-18-2014 |
20140370552 | ENDOGLUCANASE 1B (EG1B) VARIANTS - The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. | 12-18-2014 |
20140370553 | Enzymes for Starch Processing - The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides. | 12-18-2014 |
20140377811 | WHOLE-CELL BIOCATALYSTS IN THE DEGRADATION OF CELLULOSIC BIOMASS - The present invention concerns micro-organisms which present cellulases on their surface. Corresponding micro-organisms were produced with the aid of corresponding plasmids which encode a section comprising a signal peptide, a heterologous cellulase, an optional protease recognition site, a transmembrane linker and a transporter domain of an autotransporter or a variant thereof. Such micro-organisms were advantageously used in the conversion of cellulose into cellobiose and/or glucose. It was also possible to recover the micro-organisms from the reaction mixture following conversion from simple substrates. Also, a combination of various micro-organisms, which were populated with exocellulases, endocellulases and beta-glucosidases, were used to produce glucose from cellulose or wood. | 12-25-2014 |
20140377812 | PROCESS FOR THE PRODUCTION OF OPTIMISED LIQUEFIED LIGNOCELLULOSIC SUBSTRATE - The present invention concerns a production of liquefied lignocellulosic substrate by enzymatic reaction. 10% to 40% by weight dry matter of pre-treated lignocellulosic substrate is contacted, with water and enzymes at 0.1 to 60 mg of enzymes per gram of cellulose for a period of 1 to 24 hours. Over time, at least the value of one of the rheological characteristics of the reaction medium is measured. If a reduction of the value is detected over time, the following step a) is carried out:
| 12-25-2014 |
20150010957 | Alkcell Process (alkali-cellulase process) for the conversion of biomass (cellulosic material) to glucose - The Alkcell (alkali-cellulase) Process is a method for the conversion of biomass (cellulosic material) such as agricultural waste, for example, corn stover into glucose. The process uses an alkali pretreatment of biomass followed by hydrolysis with cellulase enzymes to produce glucose. The Alkcell Process uses commonly available materials and methods readily implemented near the biomass growing site. The operating conditions are mild with temperatures not above boiling water solutions, and require half-day conversion to produce glucose in water. Remaining solids can be recycled for additional glucose. An advantage of the Alkcell Process is implementation at or near the biomass growing site that avoids transporting biomass distances to special cellulose processing facilities. The glucose produced can be fermented at existing grain fermenting facilities so that special cellulose handling refineries are not required. The Alkcell Process can be configured to a manual or powered process done in individual steps or continuously. | 01-08-2015 |
20150010958 | Methods for Degrading or Converting Cellulosic Material - The present invention provides methods for degrading or converting a cellulosic material using an enzyme composition in the presence of a reducing agent. The present invention also provides methods for producing a fermentation product and methods of fermenting a cellulosic material using an enzyme composition in the presence of a reducing agent. | 01-08-2015 |
20150010959 | METHOD FOR PRODUCING SACCHARIDES CONTAINING GLUCOSE AS MAIN COMPONENT - A method for producing saccharides containing glucose as a main component is described, including degrading cellulose and/or hemicellulose with a cellulase, wherein an additive containing a protein and an amino acid and/or a yeast lysate solution is added to the cellulose and/or hemicellulose and the cellulase is used to cause an enzymatic saccharification reaction of saccharifying the cellulose and/or hemicellulose. | 01-08-2015 |
20150010960 | SOFT BIOMASS DECOMPOSITION METHOD - An object of the present invention is to provide a soft biomass decomposition method, a production method for a target substance from soft biomass, and an enzyme or group of enzymes for decomposing soft biomass. Provided is a soft biomass decomposition method, including a step of bringing an enzyme selected from specific exocellulase, endocellulase, and processive endocellulase into contact with soft biomass such as bagasse and rice straw. Also provided is a production method for a target substance from soft biomass by incorporating the soft biomass decomposition method as a step. Further provided is an enzyme or group of enzymes for decomposing soft biomass selected from specific exocellulase, endocellulase, and processive endocellulase. | 01-08-2015 |
20150010961 | POLYPEPTIDE HAVING ENDOGLUCANASE ACTIVITY - The present invention relates to a family 5 glycoside hydrolase variant having endoglucanase activity, polynucleotides encoding the family 5 glycoside hydrolase variant, vectors, host cells comprising the polynucleotides, and methods for using the family 5 glycoside hydrolase variant. | 01-08-2015 |
20150017689 | -GLUCOSIDASE - The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from | 01-15-2015 |
20150017690 | BETA-GLUCOSIDASE - The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 91% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from | 01-15-2015 |
20150017691 | -GLUCOSIDASE - The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from | 01-15-2015 |
20150017692 | BETA-GLUCOSIDASE - The present invention relates to a polypeptide which has β-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 92% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel β-glucosidase enzyme derived from | 01-15-2015 |
20150024438 | Heterologous Expression of Termite Cellulases in Yeast - The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast | 01-22-2015 |
20150024439 | Polypeptides Having Cellulolytic Enhancing Activity And Nucleic Acids Encoding Same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 01-22-2015 |
20150031091 | POLYPEPTIDES HAVING ALPHA-AMYLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention provides isolated polypeptides having alpha-amylase activity catalytic domains, carbohydrate binding domains and polynucleotide encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains. | 01-29-2015 |
20150031092 | PROCESS FOR PRETREATMENT OF THE LIGNOCELLULOSIC BIOMASS WITH A HYDRATED INORGANIC SALT MAKING IT POSSIBLE TO OBTAIN A CELLULOSIC FRACTION AND A HEMICELLULOSIC FRACTION - A method for pretreating lignocellulosic biomass by: a) acid hydrolysis with an acid solution resulting in a liquid fraction and a solid fraction, b) separating the solid fraction and the liquid fraction, c) drying the solid fraction, d) firing the dried solid fraction in a medium comprising at least one hydrated inorganic salt to obtain a solid fraction and a liquid fraction, e) separating the solid fraction and the liquid fraction obtained in d), f) optionally, treating said solid fraction obtained in e), g) enzymatic hydrolysis of said solid fraction obtained in e) and/or f), and wherein at least a portion of the liquid fraction obtained in b) is used to grow microorganisms which produce the enzymes necessary for the enzymatic hydrolysis of g). | 01-29-2015 |
20150037845 | THERMOPHILIC MUTANTS OF TRICHODERMA REESEI ENDOGLUCANASE I - The present disclosure relates to mutant thermostable glycosyl hydrolases family 7 enzymes, including mutant | 02-05-2015 |
20150037846 | METHOD FOR BIOMASS HYDROLYSIS - Methods and systems for biomass hydrolysis are disclosed. The methods use wash liquor in a sequencing process to maximize sugar yields, particularly C | 02-05-2015 |
20150037847 | METHOD OF PRODUCING A COMPOSITION CONTAINING GALACTO-OLIGOSACCHARDIES - The present invention relates to a method of producing compositions containing galacto-oligosaccharides as well as to galacto-oligosaccharide-containing compositions as such. | 02-05-2015 |
20150037848 | METHOD FOR PRODUCING SACCHARIDE - The invention provides a method for producing saccharide, the method including the following steps (1) and (2). In step (1), a cellulose raw material is heated in the presence of one or more additives selected from the group consisting of a nonionic surfactant and polyethylene glycol; and water, at a pH lower than 7, to thereby prepare a heat treatment product. In step (2), the treatment product prepared in step (1) to is subjected to a saccharification treatment by use of an enzyme. | 02-05-2015 |
20150044728 | SACCHARIFYING ENZYME COMPOSITION AND METHOD FOR PRODUCING SACCHARIFIED SOLUTION USING THE SAME - A saccharifying enzyme composition, by which excellent saccharification performance can be attained even with a low usage thereof, as well as a method for producing a saccharified solution using the same are provided. The saccharifying enzyme composition subjects lignocellulose-based biomass as a substrate to a saccharification treatment. The saccharifying enzyme composition comprises an endoglucanase not containing a cellulose-binding domain, a cellobiohydrolase containing a cellulose-binding domain, and a β-glucosidase containing a cellulose-binding domain. | 02-12-2015 |
20150044729 | BIOMASS PROCESSING SYSTEM, SACCHARIDE SOLUTION PRODUCTION METHOD USING BIOMASS FEEDSTOCK, ALCOHOL PRODUCTION METHOD - A biomass processing system includes: a hydrolysis processing unit that decomposes, under a high-temperature/high-pressure condition, biomass feedstock in a processing tank having a gas-liquid interface, and removes a lignin component and a hemicellulose component; a biomass solid content discharge unit that discharges a biomass solid content | 02-12-2015 |
20150044730 | SUGAR PREPARATION PROCESS BY ENZYMATICALLY HYDROLYZING SWEET POTATO DREG - The present invention relates to the field of biology, in particular to a sugar preparation method by using biomass sweet potato dregs for microbial fermentation as a sugar source. The liquid sugar product prepared with the method of the present invention essentially comprises the ingredient of glucose. The method of the present invention is of simple process, high specificity, good product quality and high yield, and solves the serious environmental pollution problem of the sweet potato dregs, thus having a good industrial application prospect. | 02-12-2015 |
20150044731 | PSYCHROPHILIC ENZYMES COMPOSITIONS AND METHODS FOR MAKING AND USING SAME - Enzyme compositions with enhanced enzyme activity and/or thermophilic and psychrophilic stability are described. Additionally, methods and kits for making and using the enzyme compositions are provided. | 02-12-2015 |
20150050700 | NOVEL CELLULASE - The objects of the present invention are to provide a cellulase that has activity to produce glucose by hydrolyzing crystalline cellulose and which is suitable for saccharification of crystalline cellulose, such as wood, by enzymatic method; and a method of producing glucose and alcohol from crystalline cellulose by utilizing the cellulase. | 02-19-2015 |
20150050701 | CELLULASE COMPOSITIONS HAVING IMPROVED THERMOSTABILITY AND SYNERGY - A variant Cel5a endoglucanase has increased thermostability, increased enzymatic activity and/or increased expression in a host, relative to wild type Cel5a. The improved variant Cel5a endoglucanase may be used to hydrolyze more cellulose at a higher temperature for a more efficient and cost-effective production of biofuels as compared to wild type Cel5a. A variant Cel5a endoglucanase is combined with variant Cel6a and variant Cel7a cellobiohydrolases resulting in more effective hydrolysis of cellulose. | 02-19-2015 |
20150050702 | RECOMBINANT C1 BETA-GLUCOSIDASE FOR PRODUCTION OF SUGARS FROM CELLULOSIC BIOMASS - The invention relates to expression of a recombinant C1 β-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting cellobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 β-glucosidase, operably linked to heterologous promoter, under conditions in which β-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said β-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel. | 02-19-2015 |
20150056664 | Variants of Glycoside Hydrolases - The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences. | 02-26-2015 |
20150079638 | VARIANT HUMICOLA GRISEA CBH1.1 - Disclosed are variants of | 03-19-2015 |
20150079639 | PROCESSES AND APPARATUS FOR REFINING SUGARCANE TO PRODUCE SUGARS, BIOFUELS, AND/OR BIOCHEMICALS - Conventionally, sugarcane processing avoids leaving residual sucrose in the bagasse, since the bagasse will be burned and the value of the sucrose would be lost. However, when coupled with a Green Power+® process to extract hemicelluloses, sucrose may also be extracted and recovered from the bagasse. In some variations, a process includes mechanically treating a feedstock to generate a sucrose-rich stream and lignocellulosic material that intentionally retains a significant amount of the initial sucrose in the feedstock; extracting the lignocellulosic material with steam and/or hot water to produce cellulose-rich solids and an extract liquor containing hemicellulosic oligomers and sucrose; and then hydrolyzing the hemicellulosic oligomers into a hemicellulose sugar stream. Each of the sucrose-rich stream and the hemicellulose sugar stream (containing the starting residual sucrose) may be recovered or further processed (e.g., fermented to ethanol). Similar processes are possible with energy cane, sugar beets, and energy beets. | 03-19-2015 |
20150087028 | CBH1A VARIANTS - The invention relates to recombinant expression of variant forms of | 03-26-2015 |
20150087029 | GENES ENCODING CELLULASE FOR HYDROLYZING GUAR FRACTURING FLUIDS UNDER EXTREME WELL CONDITIONS - Polynucleotide sequences encoding a thermostable cellulase and directing its increased expression are provided, and hydraulic fracturing compositions comprising such thermostable cellulase. | 03-26-2015 |
20150087030 | Pretreatment Composition for Biomass Conversion Process - A pretreatment composition and pretreatment process utilizing the composition is described. The pretreatment composition includes an aqueous organic solvent in combination with an alkaline component and an oxidizing agent. The pretreatment composition is useful for treating biomass as may be utilized in forming biofuel, paper, or other useful products. The biomass to be treated can include cellulosic biomass, lignocellulosic biomass, or a combination thereof. | 03-26-2015 |
20150087031 | METHODS FOR TREATING LIGNOCELLULOSIC MATERIALS - The present invention relates to methods of processing lignocellulosic material to obtain hemicellulose sugars, cellulose sugars, lignin, cellulose and other high-value products. Also provided are hemicellulose sugars, cellulose sugars, lignin, cellulose, and other high-value products. | 03-26-2015 |
20150093790 | THERMOSTABLE C. BESCII ENZYMES - The disclosure provides thermostable enzymes isolated from | 04-02-2015 |
20150093791 | THERMOCELLULASES FOR LIGNOCELLULOSIC DEGRADATION - Thermostable cellulase enzyme systems comprising at least one each of a thermostable endoglucanase, an exo-processive-endoglucanase, and a β-glucosidase carry out the complete, coordinated hydrolysis of crystalline cellulose to monomeric glucose. | 04-02-2015 |
20150093792 | Polypeptides Having Glucoamylase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 04-02-2015 |
20150099280 | Variants of Beta-Glucosidase - The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences. | 04-09-2015 |
20150104833 | NOVEL PROTEIN HAVING B-GLUCOSIDASE ACTIVITY AND USES THEREOF - By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic β-glucosidase was successfully identified from | 04-16-2015 |
20150111258 | PROCESS FOR THE PRODUCTION OF ORGANIC COMPOUNDS FROM PLANT SPECIES - This invention relates to a process for the production of fermentable C5-C6 sugars from oleaginous herbaceous plants comprising the steps of: a) mechanically separating the seeds from the above-ground lignocellulose biomass and breaking-up said lignocellulose biomass; b) placing the above-ground lignocellulose biomass in contact with a basic aqueous solution so as to obtain a paste containing from 10 to 50% by weight of the said lignocellulose biomass at temperature of between 10 and 95° C. for a time of between 1 minute and 24 hours; c) separating out the paste obtained in step b) in a solid fraction containing essentially hemicellulose and cellulose; in a liquid fraction containing lignin and extractables; d) subjecting the said solid fraction containing essentially hemicellulose and cellulose to enzyme hydrolysis. | 04-23-2015 |
20150111259 | Method for Making High Maltose Syrup - The present teachings provide direct conversion of granular starch into a soluble sugar composition comprising a high, very high, and/or ultra high maltose content. The method involves contacting an aqueous slurry of granular starch with an enzyme composition comprising an appropriate ratio of an alpha-amylase and a maltogenic enzyme to produce a soluble starch substrate that is enzymatically converted to a sugar composition containing higher maltose. The process may use more than one temperature to achieve the same. | 04-23-2015 |
20150118716 | Cellulolytic Enzyme Enhancement of Dry Grind Corn Processing and Ethanol Production - A method to increase ethanol production from a corn dry-mill process is described that comprises adding an enzyme preparation derived from | 04-30-2015 |
20150118717 | PREPARATION METHOD CONDUCIVE TO ENHANCING ENZYMATIC ACTIVITY OF CELLULASE - A preparation method conducive to enhancing enzymatic activity of cellulase includes the steps of preparing a first inducer, preparing preculture hyphae, producing enzymes by a single strain, feeding a second inducer, and producing enzymes by co-culture strains. Main ingredients of the first inducer and the second inducer are cellulose and lactose, respectively. The enzymatic activity of the cellulase produced is enhanced by induction of cellulose and lactose and co-culture of | 04-30-2015 |
20150118718 | STRAIN OF BACILLUS SUBTILIS AND APPLICATIONS THEREOF - The present invention is directed to a strain of | 04-30-2015 |
20150125907 | METHODS FOR INCREASING SUGAR YIELD WITH SIZE-ADJUSTED LIGNOCELLULOSIC BIOMASS PARTICLES - A method of increasing sugar yield in a bioproduct production process is provided comprising pretreating one or more size-adjusted lignocellulosic biomass (LCB) particles with a pretreatment, such as an ammonia pretreatment, wherein the one or more size-adjusted LCB particles form a substrate, with the one or more particles having an average particle size in at least one dimension greater than 5 mm. | 05-07-2015 |
20150125908 | METHOD OF PRODUCING SUGAR LIQUID - A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (3): (1): subjecting a cellulose-containing biomass to hydrothermal treatment and thereafter separating the treated cellulose-containing biomass into a hydrothermally-treated liquid and a cellulose-containing solid content; (2): adding a filamentous fungus-derived cellulase to the cellulose-containing solid content obtained in (1) to hydrolyze the cellulose and thereafter separating the hydrolysate into a saccharification residue and a sugar liquid; and (3): washing the saccharification residue obtained in (2) with the hydrothermally-treated liquid obtained in (1) to elute the filamentous fungus-derived cellulase adsorbed to the saccharification residue into the hydrothermally-treated liquid and thereafter obtaining a solution component comprising the filamentous fungus-derived cellulase by solid-liquid separation. | 05-07-2015 |
20150125909 | Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And An Organic Compound And Uses Thereof - The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and an organic compound. The present invention also relates to methods of using the compositions. | 05-07-2015 |
20150140612 | Methods for Increasing Enzymatic Hydrolysis of Cellulosic Material - The present invention relates to methods for increasing hydrolysis of a pretreated cellulosic material, comprising subjecting the pretreated cellulosic material to a cellulolytic enzyme composition; a polypeptide having cellulolytic enhancing activity; a Peroxidase; and a nonionic surfactant and/or cationic surfactant, at conditions suitable for hydrolyzing the pretreated lignocellulosic material. The invention also relates to processes for producing a fermentation product comprising a hydrolysis step of the invention and a composition suitable for use in a method of the invention. | 05-21-2015 |
20150147787 | PROCESS OF PRODUCING SUGAR SOLUTION - A method produces a sugar liquid from a cellulosic biomass material, which sugar liquid has improved fermentability. The method produces a sugar liquid by: adding an alkali(s) to a concentrated cellulosic biomass sugar liquid to adjust the pH to not less than 7 to precipitate an insoluble substance(s) containing at least magnesium; and performing filtration through a microfiltration membrane to remove the insoluble substance(s), to obtain a sugar liquid as a permeate. | 05-28-2015 |
20150291928 | APPARATUS AND METHOD FOR SIMULTANEOUS OPERATION OF REFINING CELLULOSIC BIOMASS AND MIXING ENZYMES - An apparatus comprises: a hopper for feeding biomass; a horizontal extruder comprising a cylindrical housing and a rotatable screw mounted in the housing, in which one side of the housing is connected to the hopper, and an outlet provided at the other side of the housing is connected to an inlet for feeding biomass into a refiner; and a refiner comprising a stator disc having a cooling water space formed on the outer peripheral surface thereof, one or more enzyme feeding holes provided in a refining region adjacent to the circumference of the stator disc, and a biomass inlet provided at the central portion thereof, and a refining disc which is disposed so as to form a gap with the inner surface of the stator disc and to have a refining region opposite to that of the stator disc and is rotated by a separate motor. | 10-15-2015 |
20150291947 | CELLOBIOHYDROLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 10-15-2015 |
20150291989 | REACTOR FOR CONTINUOUS SACCHARIFICATION OF HIGH-SOLID BIOMASS - A reactor for continuous saccharification of biomass having a high solid content comprises perforated plates and stirring means, in which the perforated plates includes nozzles for feeding additives, including a saccharification enzyme, and a sensor, which communicate with the holes of the perforated plates. | 10-15-2015 |
20150291990 | Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 10-15-2015 |
20150299683 | EGVII Endoglucanase and Nucleic Acids Encoding the Same - The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same. | 10-22-2015 |
20150299749 | PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS - The invention relates to a process for the preparation of a sugar product from ligno-cellulosic material, comprising the following steps:
| 10-22-2015 |
20150299751 | METHOD OF ENZYMATIC TREATMENT OF A SOLID LIGNOCELLULOSIC MATERIAL - A method of treating a solid lignocellulosic material ( | 10-22-2015 |
20150299752 | Milling Process - Process for treating crop kernels, comprising the steps of: a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of a beta-xylosidase, wherein step c) is performed before, during or after step b). | 10-22-2015 |
20150307909 | Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins - The present invention relates to methods for degrading or converting a cellulosic material and for producing substances from the cellulosic material. | 10-29-2015 |
20150315560 | VARIANTS OF CELLOBIOHYDROLASES - Disclosed are a number of homologs and variants of | 11-05-2015 |
20150315622 | Methods of Saccharifying and Fermenting a Cellulosic Material - The invention relates to methods of saccharifying a cellulosic material comprising subjecting the cellulosic material to a cellulolytic enzyme composition and a GH61 polypeptide, and optionally a catalase in the presence of dissolved oxygen at a concentration in the range of 0.5 to 10% of the saturation level. The invention also related to methods of producing desired fermentation products, such as ethanol, using a method including a saccharification step of the invention. | 11-05-2015 |
20150322398 | BIOMASS PROCESSING DEVICE AND PROCESSING METHOD - A biomass treatment device and a biomass treatment method, in which contamination is prevented and a final product can be highly concentrated. The biomass treatment device includes a pretreatment device for pretreating a biomass, a slurrying device for slurrying the pretreated biomass, and a saccharification device for saccharifying the slurried biomass with a saccharifying enzyme. A return line for returning a part of an internal fluid in the saccharification device to the slurrying device is provided, and the pretreated biomass from the pretreatment device and the internal fluid returned through the return line are mixed in the slurrying device so as to generate biomass slurry. | 11-12-2015 |
20150322471 | PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL - The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises
| 11-12-2015 |
20150329841 | CELLULOSE-DEGRADING ENZYME COMPOSITION COMPRISING GH16 - The present invention relates to a cellulose-degrading enzyme composition comprising one or more cellobiohydrolase and/or endoglucanase enzyme, and an effective amount of an isolated GH16 polypeptide, where the presence of the isolated GH16 polypeptide in the enzyme composition increases the rate or extent of degradation of a cellulosic substrate compared to an equivalent dosage of a corresponding cellulose-degrading enzyme composition lacking the at least one isolated GH16 polypeptide. The present invention also relates to a method for producing fermentable sugars from a cellulosic substrate using the above cellulose-degrading enzyme composition and to genetically modified microbes for produced the above cellulose-degrading enzyme composition. | 11-19-2015 |
20150329842 | COMPOSITIONS AND METHODS COMPRISING CELLULASE VARIANTS WITH REDUCED AFFINITY TO NON-CELLULOSIC MATERIALS - The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions. | 11-19-2015 |
20150329843 | HOST CELL CAPABLE OF PRODUCING ENZYMES USEFUL FOR DEGRADATION OF LIGNOCELLULOSIC MATERIAL - The invention relates to a host cell comprising at least four different heterologous polynucleotides chosen from the group of polynucleotides encoding cellulases, hemicellulases and pectinases, wherein the host cell is capable of producing the at least four different enzymes chosen from the group of cellulases, hemicellulases and pectinases, wherein the host cell is a filamentous fungus and is capable of secretion of the at least four different enzymes. This host cell can suitably be used for the production of an enzyme composition that can be used in a process for the saccharification of cellulosic material. | 11-19-2015 |
20150329887 | INTEGRATED BIOREFINERY - This invention relates to the operation of a biorefinery for manufacturing either biofuels or renewable chemical feedstock using lignocellulosic biomass as a source of carbon. The present invention provides a cost-effective process for pretreating lignocellulosic biomass in the recovery of fermentable sugars. More specifically, the present invention describes an integrated approach for efficiently recovering and using six-carbon and five-carbon sugars along with value-added oligosaccharides such as xylooligosaccahrides from lignocellulosic biomass so that the cost of manufacturing biofuels and renewable chemical feedstock is substantially lowered. | 11-19-2015 |
20150329888 | PROCESS FOR THE PRODUCTION OF SOLUTIONS OF SUGARS AND ALCOHOLS FROM LIGNOCELLULOSIC BIOMASS WITH COMPLEMENTARY TREATMENT OF SOLID RESIDUE WITH A HYDRATED INORGANIC SALT - The present invention describes a process for the production of solutions of sugars and alcohols from lignocellulosic biomass, comprising a pre-treatment, a step for enzymatic hydrolysis and alcoholic fermentation, a step for extraction of the alcohol produced, a step for separation of the solid residue, an optional step for washing and drying, followed by a step for digestion of the solid residue in a medium comprising at least one hydrated inorganic salt with formula MX | 11-19-2015 |
20150329889 | PROCESS FOR ENZYMATIC HYDROLYSIS OF CELLULOSE - Cellulose from lignocellulosic biomass which also contains protein, such as grasses and straws, can be enzymatically converted to valuable products such as glucose at lower enzyme consumption by: (a) extracting the biomass with water at 15-75° C. to produce an extracted biomass and a protein-containing aqueous extract; (b) mechanically, chemically and/or thermally pretreating cellulose-containing-biomass, in particular by organosolv treatment; and (c) contacting biomass pretreated in step (b) with: (i) an enzyme capable of hydrolysing cellulose; and (ii) the protein-containing aqueous extract obtained in step (a). | 11-19-2015 |
20150344858 | NOVEL MANNANASE, COMPOSITIONS AND METHODS OF USE THEREOF - The present compositions and methods relate to an endo-beta-mannanase cloned from | 12-03-2015 |
20150344867 | HIGHLY POTENT CELLULOLYTIC ENZYME PREPARATIONS AND PROCESSES FOR PRODUCING SAME - Compositions comprising unprocessed cell pellets of a cellulosome-producing microorganism grown on cellulosic biomass are provided. Further provided are methods for producing the compositions and uses thereof in hydrolysis of cellulosic substrates. In particular, the compositions advantageously contain extracellular beta-glucosidase, either expressed on the cells themselves or extrinsically added to the cell pellets. | 12-03-2015 |
20150344914 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation. | 12-03-2015 |
20150344922 | COMPOSITIONS AND METHODS OF USE - The present compositions and methods relate to a beta-mannanase from | 12-03-2015 |
20150344923 | COMPOSITIONS AND METHODS OF USE - The present compositions and methods relate to a beta-mannanase from | 12-03-2015 |
20150344924 | POLYMER ENHANCEMENT OF ENZYMATIC ACTIVITY - Provided herein are methods for enhancing enzymatic activity using certain polymers that may be optionally attached to an enzyme. The polymers may be thermally-responsive polymers, including poly N-isopropylacrylamide or poly N-isopropylmethacrylamide. The polymer may also be a copolymer with at least two different monomer residues. The monomer residues may have a structure of formula (I): | 12-03-2015 |
20150352540 | MULTI-LIGAND METAL COMPLEXES AND METHODS OF USING SAME TO PERFORM OXIDATIVE CATALYTIC PRETREATMENT OF LIGNOCELLULOSIC BIOMASS - A homogeneous catalyst is provided comprising one or more metals; and at least two metal coordinating ligands wherein the homogeneous catalyst is a multi-ligand metal complex_adapted for use with an oxidant in an oxidation reaction to catalytically pretreat lignocellulosic biomass. In one embodiment, the homogenous catalyst is copper (II) 2,2′ bipyridine ethylenediamine (Cu(bpy)en). Related methods are also disclosed. | 12-10-2015 |
20150353881 | DEVICE THAT PRODUCES SUGAR SOLUTION AND METHOD OF PRODUCING SUGAR SOLUTION - A device that produces a sugar solution from cellulose-containing biomass includes: a horizontal reaction tank that includes a stirring shaft provided along a horizontal direction in the horizontal reaction tank and a stirring blade provided to the stirring shaft, the horizontal reaction tank being configured to obtain a saccharified slurry by reacting the cellulose-containing biomass with a saccharification enzyme by stirring the cellulose-containing biomass and the saccharification enzyme; a vertical reaction tank configured to obtain a saccharified liquid by saccharifying the saccharified slurry; a saccharified slurry feed line that connects the horizontal reaction tank and the vertical reaction tank; and a warming part provided around the horizontal reaction tank or on a wall surface of the horizontal reaction tank and heats the horizontal reaction tank. | 12-10-2015 |
20150353977 | METHOD FOR PREPARING FERMENTABLE SUGAR FROM WOOD-BASED BIOMASS - The present invention relates to a method for preparing, from wood-based biomass, a high concentration of fermentable sugar which can be effectively used in culturing various industrial fermented bacteria. According to the method of the present invention, biomass can be extracted by hot water prior to a pre-treatment so as to remove extractible substances such as mineral salts to thus minimize the content of impurities in raw materials for an enzymatic saccharification. The biomass from which substances extractible by hot water are removed is pre-treated in the condition where xylan yield rate is maximized, thus achieving maximum inhibition of the generation of over-decomposed products of sugar. Subsequently, fermentable sugar for culturing various industrial fermented bacteria can be prepared in an inexpensive manner by only concentrating, using a separator membrane, the sugar solution obtained by an enzymatic saccharification of the pre-treated solid content obtained by a solid-liquid separation without washing the solid content with water. | 12-10-2015 |
20150361408 | CARBOHYDRATE DEGRADING POLYPEPTIDE AND USES THEREOF - The invention relates to a polypeptide having hemicellulase activity. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins. | 12-17-2015 |
20150368629 | Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 12-24-2015 |
20150368683 | Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 12-24-2015 |
20150368684 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. Two or more sugars can be produced and these can be further processed and purified. For example, a mixture of the two or more sugars can be selectively fermented to leave one or more sugars in the mixture along with a product. The unfermented sugar may be fermented with a different fermenting system and produce a second product. | 12-24-2015 |
20150376589 | VARIANT CBHII POLYPEPTIDES WITH IMPROVED SPECIFIC ACTIVITY - The present disclosure relates to variant CBH II polypeptides that have improved specific activity, and compositions, e.g., cellulase compositions, comprising variant CBH II polypeptides. The variant CBH II polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH II polypeptides and host cells that recombinantly express the variant CBH II polypeptides. | 12-31-2015 |
20150376590 | Thermotolerant Beta-Glucosidase Variants - The present disclosure relates to variant β-glucosidase polypeptides that have enhanced thermostability, and compositions, e.g., cellulase compositions, comprising variant β-glucosidase polypeptides. The variant β-glucosidase polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant β-glucosidase polypeptides and host cells that recombinantly express the variant β-glucosidase polypeptides. | 12-31-2015 |
20150376668 | METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS TERREUS AND ISOAMYLASE FOR SACCHARIFICATION - A fungal alpha-amylase is provided from | 12-31-2015 |
20150376669 | METHOD OF PRODUCING SUGAR SOLUTION - A method of producing sugar solution by repeating a sugar solution production process includes steps (1) to (3), wherein a wash solution obtained in step (4) of washing a separation membrane after step (3) is used for step (1) of subsequent sugar solution production processes:
| 12-31-2015 |
20150376833 | METHOD AND APPARATUS FOR TREATMENT OF BIOMASS SUBSTRATES - A system or method for the treatment of biomass comprising mixing a biomass with an ionic liquid (IL) to swell the biomass and electromagnetic (EM) heating, preferably radio frequency (RF) heating or infrared (IR) heating, said biomass. Additionally, a method of acidolysis of biomass comprising mixing biomass in an ionic liquid (IL) to swell the biomass; adding an acid, to lower the pH of the biomass below pH 7; applying radio frequency (RF) heating or infrared (IR) heating to the biomass to heat to a target temperature range; applying ultrasonic heating, electromagnetic (EM) heating, convective heating, conductive heating or combinations thereof, to the biomass to maintain the biomass at a target temperature range; washing the treated biomass; and recovering sugars and release lignin. | 12-31-2015 |
20160002689 | PROCESSES UTILIZING PULP AND PAPER MILL EQUIPMENT FOR ENZYMATIC HYDROLYSIS OF CELLULOSIC FIBERS TO GLUCOSE - A method for the production of alcohol and other bioproducts hemicelluloses extracted from biomass prior to thermal conversion of the biomass to energy. The process can be integrated with the host plant process to minimize the energy loss from extracting hemicelluloses. Also disclosed is a Stepwise enzymatic break down of cellulose fibers from a pulping operation which is performed with the redeployment of equipment and vessels contained within typical existing pulp and paper manufacturing mills. The preferred feedstock is highly delignified pulp from acid or alkaline pulping process or from bleaching stage. | 01-07-2016 |
20160002690 | Milling Process - The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of an enzyme composition comprising: i) a protease, and ii) a cellulolytic composition, wherein step c) is performed before, during or after step b). | 01-07-2016 |
20160002692 | EXPRESSION OF BETA-GLUCOSIDASES FOR HYDROLYSIS OF LIGNOCELLULOSE AND ASSOCIATED OLIGOMERS - The present invention provides for heterologous expression of beta-glucosidase (BGL) polypeptides encoded by | 01-07-2016 |
20160010051 | BIOMASS TREATMENT SYSTEM, SACCHARIDE SOLUTION PRODUCING PROCESS USING BIOMASS AS RAW MATERIAL, AND ORGANIC RAW MATERIAL PRODUCING PROCESS | 01-14-2016 |
20160010114 | CONSORTIUM AND PREPARATION OF MICROORGANISMS FOR CATALYZING CELLULOSE HYDROLYSIS, PREPARATION FOR METHANE FERMENTATION SUPPLEMENTATION, COMBINATION PREPARATION, USE THEREOF AND METHOD USING THE SAME | 01-14-2016 |
20160010128 | METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS TERREUS AND PULLULANASE FOR SACCHARIFICATION | 01-14-2016 |
20160010129 | VARIANT ALPHA-AMYLASES FROM BACILLUS SUBTILIS AND METHODS OF USE, THEREOF | 01-14-2016 |
20160010130 | SUGAR-SOLUTION PRODUCTION METHOD | 01-14-2016 |
20160010131 | METHOD FOR PRETREATING BIOMASS, AND METHOD FOR PRODUCING SUGAR CONTAINING GLUCOSE AS MAIN COMPONENT | 01-14-2016 |
20160017303 | ALPHA-AMYLASE COMBINATORIAL VARIANTS - Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing. | 01-21-2016 |
20160017304 | ALPHA-AMYLASE COMBINATORIAL VARIANTS - Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing. | 01-21-2016 |
20160017305 | ALPHA-AMYLASE COMBINATORIAL VARIANTS - Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing. | 01-21-2016 |
20160017306 | Methods for Using Polypeptides Having Cellobiohydrolase Activity - The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 01-21-2016 |
20160017390 | FUCOSIDASE FROM BACTEROIDES AND METHODS USING THE SAME - The present disclosure relates to an α-fucosidase having α-(1,2), α-(1,3), α-(1,4), and α-(1,6) fucosidase activity. The present disclosure also relates to the compositions comprising the α-fucosidase, and the methods of producing and using the α-fucosidase in cleaving α-(1,2), α-(1,3), α-(1,4), and/or α-(1,6)-linked fucoses in the glycoconjugates. | 01-21-2016 |
20160024486 | THERMOSTABLE XYLANASE BELONGING TO GH FAMILY 10 - A thermostable xylanase including a xylanase catalytic domain, the xylanase catalytic domain including:
| 01-28-2016 |
20160024545 | METHODS OF CONVERTING LIGNING AND USES THEREOF - Method for creating valuable products from lignocellulosic biomass comprising sequential pretreatment of lignocellulosic biomass with ionic liquid followed by hydrothermal processing of the lignin. | 01-28-2016 |
20160032265 | THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3 - A thermostable β-xylosidase including a β-xylosidase catalytic domain, the β-xylosidase catalytic domain including:
| 02-04-2016 |
20160032336 | Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 02-04-2016 |
20160032339 | CELLULOSIC ENZYME RECYCLING FROM SEPARATION OF SACCHARIFIED BIOMASS - The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatement. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose. | 02-04-2016 |
20160032340 | Method and Compositions for Improved Lignocellulosic Material Hydrolysis - A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of | 02-04-2016 |
20160032341 | PROCESSING BIOMASS AND PETROLEUM CONTAINING MATERIALS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation. | 02-04-2016 |
20160040145 | Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 02-11-2016 |
20160040202 | METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND PULLULANASE FOR SACCHARIFICATION - A fungal alpha-amylase is provided from | 02-11-2016 |
20160040203 | GLYCOSYL HYDROLASE XYLANASES, COMPOSITIONS AND METHODS OF USE FOR EFFICIENT HYDROLYSIS AND PROCESSING OF XYLAN - The invention provides a unique subset of GH30 subfamily 8 xylanases (GH30-8) with endo-β-1,4-xylanase activity, compositions comprising an effective amount of the GH30-8 xylanases, methods of synthesis and methods of use thereof. | 02-11-2016 |
20160040205 | Enzymes for Starch Processing - The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides. | 02-11-2016 |
20160040259 | METHOD FOR MIXED BIOMASS HYDROLYSIS - Methods and systems are disclosed for the hydrolysis of mixed biomass. The methods include forming a mixture of at least two modified biomass feedstocks to achieve various benefits, such as maximizing sugar yields and minimizing the formation of degradation products. | 02-11-2016 |
20160040354 | Treatment - The present invention relates to an improved method for treating a lignocellulose biomass in order to dissolve the lignin therein, while the cellulose does not dissolve. The cellulose pulp obtained can be used to produce glucose. In addition the lignin can be isolated for subsequent use in the renewable chemical industry as a source for aromatic platform chemicals. | 02-11-2016 |
20160046917 | Cellobiohydrolase Variants and Polynucleotides Encoding Same - The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 02-18-2016 |
20160046918 | MUTANT OF CELLULASE-PRODUCING MICROORGANISM, PRODUCTION METHOD OF CELLULASE AND PRODUCTION METHOD OF CELLO-OLIGOSACCHARIDE - An object is to provide a mutant of a cellulase-producing microorganism which produces a cellulase capable of preferentially producing a cello-oligosaccharide during the selective production of the cello-oligosaccharide through enzymolysis of a cellulosic material in the presence of the cellulase, a method for producing the cellulase and a method for producing a cello-oligosaccharide using the cellulase. The present invention relates to a mutant of a cellulase-producing microorganism, in which cellobiohydrolase and β-glucosidase genes are disrupted. | 02-18-2016 |
20160060664 | METHOD FOR IMPROVING THE FERMENTABLE SUGAR YIELD FROM LIGNOCELLULOSIC - The invention relates to processes for the conversion of biomass into carbohydrates, notable fermentable sugars. It provides means and methods for increasing the yield of enzymatic digestion of a biomass, in particular in those cases where cellulose is converted into sugars using a cellulose converting enzyme. More in particular, the invention relates to a method for producing a fermentable sugar from a lignocellulosic material wherein the lignocellulosic material is contacted with a laccase and an enzyme capable of degrading cellulose, either simultaneously or in a sequentially deferred fashion, wherein the laccase is the | 03-03-2016 |
20160060665 | HEMICELLULASE ENRICHED COMPOSITIONS FOR ENHANCING HYDROLYSIS OF BIOMASS - Described are compositions and methods relating to cellulase/hemicellulase enzyme blends for improving the enzymatic hydrolysis of cellulosic and hemicellulosic materials, as commonly found in biomass. | 03-03-2016 |
20160060666 | THERMOSTABLE XYLANASE BELONGING TO GH FAMILY 10 - A thermostable xylanase having a xylanase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having xylanase activity at least under conditions of 85° C. and pH 6.0, or (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having xylanase activity at least under conditions of 85° C. and pH 6.0. | 03-03-2016 |
20160060667 | CONTINUOUS COUNTERCURRENT ENZYMATIC HYDROLYSIS OF PRETREATED BIOMASS AT HIGH SOLIDS CONCENTRATIONS - The invention provides a continuous process for enzymatic hydrolysis of pretreated biomass, the process comprising: providing a pretreated lignocellulosic biomass feed material containing cellulose; introducing the pretreated lignocellulosic biomass feed material to a mechanical-treatment unit containing one or more decompression regions configured to release pressure; introducing a liquid solution containing cellulase enzymes to one or more decompression regions in the mechanical-treatment unit, wherein the liquid solution enters void spaces between fibers of the pretreated lignocellulosic biomass feed material, to form enzyme-containing cellulose-rich solids; and retaining the enzyme-containing cellulose-rich solids under effective hydrolysis conditions to hydrolyze at least some of the cellulose to glucose. Various apparatus configurations are disclosed for the mechanical-treatment unit. | 03-03-2016 |
20160060668 | BIOPROCESSING - Functionalized substrate materials, for example inorganic particles and/or synthetic polymeric particles, are used to enhance bioprocesses such as saccharification and fermentation. | 03-03-2016 |
20160060669 | GLUCOSE PRODUCTION METHOD AND GLUCOSE PRODUCED BY SAID METHOD - A glucose production method produces glucose by using excrement from living matter that is disposed as waste, without consuming food resources or agricultural resources, which can stably provide glucose at low cost without being affected by crop yields. | 03-03-2016 |
20160060670 | Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 03-03-2016 |
20160068878 | Methods of Preconditioning Cellulosic Material - The invention relates to methods of preconditioning unwashed pretreated cellulosic material using a combination of phenol oxidizing enzyme and hemicellulase. The invention also relates to processes of producing sugars and fermentation products including a preconditioning method of the invention. | 03-10-2016 |
20160076015 | THERMOSTABLE BETA-GLUCOSIDASE - A thermostable β-glucosidase including a β-glucosidase catalytic domain, the β-glucosidase catalytic domain including:
| 03-17-2016 |
20160076016 | THERMOSTABLE BETA-GLUCOSIDASE - A thermostable β-glucosidase including a β-glucosidase catalytic domain, the β-glucosidase catalytic domain including:
| 03-17-2016 |
20160076017 | THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3 - A thermostable β-xylosidase, having a β-xylosidase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0, or (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0. | 03-17-2016 |
20160083761 | Polypeptides Having Xylanase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity, catalytic domains, carbohydrate binding modules and encoding the polypeptides, catalytic domains or carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules. | 03-24-2016 |
20160083762 | LIQUEFACTION PROCESSES - The present invention provides a process of liquefying starch-containing material comprising treating said starch-containing material with at least one alpha-amylase and a maltogenic amylase or at least one amylase and at least one esterase. | 03-24-2016 |
20160090614 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation. | 03-31-2016 |
20160097043 | NOVEL RECOMBINANT CELLULASE AND USE THEREOF - The present invention provides | 04-07-2016 |
20160097068 | Enhancing Enzymatic Hydrolysis by Enzymatic Preconditioning - The invention relates to methods of preconditioning pretreated lignocellulose-containing material in the presence of a combination of laccase and beta-glucosidase. The invention also relates to processes of producing sugars and fermentation products including a step of preconditioning pretreated lignocellulose-containing material in the presence of a combination of laccase and beta-glucosidase. The invention also relates to compositions suitable for preconditioning. | 04-07-2016 |
20160102299 | POLYPEPTIDES ENCODING MUTATED MANNANASES WITH IMPROVED CATALYTIC EFFICIENCY - A polypeptide which is a mutated mannanase, whose sequence is derived from a native mannanase of the filamentous ascomycete coprophile fungus | 04-14-2016 |
20160108384 | Amylases, Nucleic Acids Encoding Them and Methods for Making and Using Them - In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars. | 04-21-2016 |
20160108385 | HYPERTHERMOSTABLE ENDOGLUCANASE BELONGING TO GH FAMILY 12 - A hyperthermostable endoglucanase including an endoglucanase catalytic domain, the endoglucanase catalytic domain including:
| 04-21-2016 |
20160115464 | CARBOHYDRATE BINDING MODULES WITH REDUCED BINDING TO LIGNIN - Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolysing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolysing cellulose or hemicellulose in the presence of lignin. | 04-28-2016 |
20160115465 | COMPOSITIONS AND METHODS OF USE - The present compositions and methods relate to a beta-mannanase from | 04-28-2016 |
20160115509 | TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF - Fungal glucoamylases from | 04-28-2016 |
20160122734 | VARIANTS OF CELLOBIOHYDROLASES - Disclosed are a number of homologs and variants of | 05-05-2016 |
20160122735 | VARIANTS OF CELLOBIOHYDROLASES - Disclosed are a number of homologs and variants of | 05-05-2016 |
20160122737 | XYLANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to xylanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 05-05-2016 |
20160122791 | HYPERTHERMOSTABLE ENDOGLUCANASE BELONGING TO GH FAMILY 12 - A hyperthermostable endoglucanase including an endoglucanase catalytic domain, the endoglucanase catalytic domain including:
| 05-05-2016 |
20160130369 | FRACTIONATION PROCESSES FOR HIGH-ASH LIGNOCELLULOSIC BIOMASS FEEDSTOCKS - A process for fractionating high-ash lignocellulosic biomass is provided, comprising: (a) providing a biomass feedstock comprising lignocellulosic material and ash, some of which may be removed before digestion; (b) contacting the feedstock with a digestor solution comprising a first organic solvent, an acid catalyst, and water, under effective digestor conditions and controlled acidity to produce intermediate solids and a first liquid phase comprising hemicelluloses and lignin; (c) separating the first liquid phase from the intermediate solids; (d) washing the intermediate solids with a wash solution comprising a second organic solvent and water, under effective washing conditions to produce cellulose-rich solids and a second liquid phase comprising hemicelluloses and lignin; and (e) separating the cellulose-rich solids from the second liquid phase. The solvent type is optimized to control acidity in the digestor. Controlling the acidity may also include adjusting digestor pH to a value between about 0.5 and about 2.0. | 05-12-2016 |
20160130572 | ALPHA AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 05-12-2016 |
20160130620 | BIOCATALYST FOR SIMULTANEOUSLY DEGRADING LIGNIN AND CELLULOSE, AND METHOD FOR MANUFACTURING HYDROLYSATE AND BIOFUEL USING THE SAME - The present disclosure relates to a method for simultaneously degrading lignin and cellulose and for boosting effect on the cellulase activity using a specific catalyst. Since the present disclosure allows for the preparation of sugars by degrading not only lignin but also cellulose and hemicellulose using the enzymes which were previously known only as lignin-degrading biocatalysts, it provides the advantage that the preparation of a hydrolysate as a source material for the production of biofuels or biochemicals from lignocellulosic biomass can be simplified and facilitated. As a result, the present disclosure can reduce enzyme cost and can provide improved production efficiency by simplifying the biofuel production process. | 05-12-2016 |
20160138218 | PROCESS AND APPARATUS FOR BIOMASS CLEANING IN LIGNOCELLULOSIC BIOREFINERIES - A wet process for cleaning biomass is disclosed, comprising: introducing biomass feedstock to a vibrating separator, to generate an overflow stream and an underflow stream, wherein the overflow stream comprises biomass and large grit, and wherein the underflow stream comprises fines and small grit; introducing the overflow stream to a kinetic separator, to generate an intermediate biomass stream and a large-grit stream; introducing the underflow stream and elutriation water to a hydroclone separator, to generate a wet biomass-fines stream and a small-grit stream; separating water contained in the wet biomass-fines stream and recycling it as elutriation water, to generate a biomass fines stream; and combining the biomass fines stream with the intermediate biomass stream, thereby generating clean biomass. An alternative embodiment for a dry process to clean biomass is also disclosed. The clean biomass may be used in a wide variety of biorefining processes. | 05-19-2016 |
20160145663 | Compositions for saccharification of cellulosic material - The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof. | 05-26-2016 |
20160152736 | IMPROVED PROCESS FOR THE ORGANOSOLV TREATMENT OF LIGNOCELLULOSIC BIOMASS | 06-02-2016 |
20160152779 | PROCESSES FOR PRODUCING LIGNIN-BASED ENZYMATIC HYDROLYSIS ENHANCERS, AND COMPOSITIONS PRODUCED THEREFROM | 06-02-2016 |
20160160252 | POLYMERS IN BIOMASS SACCHARIFICATION BIOPROCESS - Methods and systems for increasing the yield of sugars from a biomass, such as a lignocellulosic biomass, are described. A non-ionic organic polymer is contacted with the biomass during the saccharification reaction, and the hydrolyzed mixture is separated using a filter into a permeate and a retentate, where the non-ionic organic polymer is present in the retentate. The retentate with the polymer is recycled to the hydrolysis mixture, which increased the yield of sugars using less saccharification enzymes. The methods thus allow for increased cost savings by reducing the amount of enzymes required to convert the biomass to sugars. | 06-09-2016 |
20160160254 | PLANTS EXPRESSING CELL WALL DEGRADING ENZYMES AND EXPRESSION VECTORS - Vectors for expression of proteins in plants are described. The proteins may be enzymes and the enzymes can be but are not limited to cell wall degrading enzymes. A number of plants designed to express specific cell wall degrading enzymes are provided. The plants may have industrial and/or agricultural applications. Methods and materials for making the expression vectors and for making the plants are provided. Processes for which the plants could be used in industrial and agricultural applications are also provided. | 06-09-2016 |
20160167010 | PROCESSING BIOMASS | 06-16-2016 |
20160168548 | THERMOSTABLE BETA-XYLOSIDASE | 06-16-2016 |
20160168549 | THERMOSTABLE CELLOBIOHYDROLASE | 06-16-2016 |
20160168550 | VARIANT ENZYMES | 06-16-2016 |
20160168551 | Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same | 06-16-2016 |
20160168553 | THERMOSTABLE BETA-GLUCOSIDASE | 06-16-2016 |
20160168608 | A PREPARATION METHOD OF GALACTOOLIGOSACCHARIDES WITH ENHANCED GALACTOSYLLACTOSE WHICH IS A INGREDIENT OF MOTHER'S MILK | 06-16-2016 |
20160168609 | ENHANCED CELLULOSE DEGRADATION | 06-16-2016 |
20160177279 | NOVEL GLYCOSYL HYDROLASE ENZYMES AND USES THEREOF | 06-23-2016 |
20160177281 | VARIANT ENZYMES | 06-23-2016 |
20160177357 | METHOD FOR PRETREATING CELLULOSE-CONTAINING BIOMASS, METHOD FOR PRODUCING BIOMASS COMPOSITION FOR SACCHARIFICATION USE, AND METHOD FOR PRODUCING SUGAR | 06-23-2016 |
20160177358 | PROCESSING BIOMASS | 06-23-2016 |
20160186155 | VARIANT ENZYMES - Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described. | 06-30-2016 |
20160186156 | ARTIFICIAL CELLULOSOMES COMPRISING MULTIPLE SCAFFOLDS AND USES THEREOF IN BIOMASS DEGRADATION - Multi-enzyme complexes comprising an array of scaffold subunits designed for efficient integration of a plurality of carbohydrate-active enzymes are provided. | 06-30-2016 |
20160186221 | COMPOSITIONS AND METHODS OF USE - The present compositions and methods relate to a beta-mannanase from | 06-30-2016 |
20160186222 | FUNGAL XYLANASES AND XYLOSIDASES - The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are | 06-30-2016 |
20160194619 | METHODS OF COMBINED BIOPROCESSING AND RELATED MICROORGANISMS, THERMOPHILIC AND/OR ACIDOPHILIC ENZYMES, AND NUCLEIC ACIDS ENCODING SAID ENZYMES | 07-07-2016 |
20160194675 | PRODUCTION OF GALACTO-OLIGOSACCHARIDES | 07-07-2016 |
20160201043 | CELLULASE ENZYMES HAVING A MODIFIED LINKER AND REDUCED LIGNIN BINDING | 07-14-2016 |
20160201104 | VARIANTS OF CELLOBIOHYDROLASES | 07-14-2016 |
20160251689 | MODIFIED -GALACTOSIDASE | 09-01-2016 |
20160251690 | METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS | 09-01-2016 |
20160376621 | FED BATCH PROCESS FOR BIOCHEMICAL CONVERSION OF LIGNOCELLULOSIC BIOMASS TO ETHANOL - A method for optimization of a fed batch hydrolysis process wherein the hydrolysis time is minimized by controlling the feed addition volume and/or batch addition frequency of the prehydrolysate and optionally also the enzyme feed. The increase over time in hydrolysate consistency and volume and/or concentration of sugars released in the reactor, so that the enzymatic hydrolysis is controlled, significantly reduces the impact of cellulase feedback inhibition, especially for enzyme contents lower than 1%. The overall time to reach conversion of the total prehydrolysate feed is reduced significantly where the batch addition frequency is equal to one batch each time 70% to 90%, preferably 80%, conversion of the previous batch is reached in the reaction mixture. At an enzyme load of 0.3% in the reaction mixture, the optimum frequency each time 80% conversion was reached was found to be one batch every 80 to 105 minutes. | 12-29-2016 |
20160376622 | Cellulolytic enzyme compositions and uses thereof - The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions. | 12-29-2016 |
20170233705 | MODIFIED -GALACTOSIDASE | 08-17-2017 |
20170233707 | COMPOSITIONS COMPRISING BETA-MANNANASE AND METHODS OF USE | 08-17-2017 |
20170233753 | Enzymes Manufactured in Transgenic Soybean for Plant Biomass Engineering and Organopollutant Bioremediation | 08-17-2017 |
20170233772 | COOLING AND PROCESSING MATERIALS | 08-17-2017 |
20180023066 | G24 GLUCOAMYLASE COMPOSITIONS AND METHODS | 01-25-2018 |
20180023106 | IN-SITU BIOSTIMULATION OF THE HYDROLYSIS OF ORGANIC MATTER FOR OPTIMIZING THE ENERGY RECOVERY THEREFROM | 01-25-2018 |
20180023107 | METHODS FOR MITIGATING THE INHIBITORY EFFECTS OF LIGNIN AND SOLUBLE PHENOLICS FOR ENZYMATIC CONVERSION OF CELLULOSE | 01-25-2018 |