Class / Patent application number | Description | Number of patent applications / Date published |
435097000 | Produced by the action of a glycosyl transferase (e.g., alpha, beta, gamma-cyclodextrins by the action of glycosyl transferase on starch, etc.) | 39 |
20090104666 | Hybrid Glycosylated Products and Their Production and Use - The present invention relates to hybrid glycosylated products, and in particular, to natural products such as polyketides and glycopeptides, and to processes for their preparation. The invention is particularly concerned with recombinant cells in which a cloned microbial glycosyltransferase can be conveniently screened for its ability to generate specific glycosylated derivatives when supplied with polyketide, peptide, or polyketide-peptides as substrates. The invention demonstrates that cloned glycosyltransferases when rapidly screened for their ability to attach a range of activated sugars to a range of exogenously supplied or endogenously generated aglycone templates, show a surprising flexibility towards both aglycone and sugar substrates, and that this process allows the production of glycosylated polyketides in good yield. This overcomes the problem not only of supplying novel sugar attachments to individual polyketides, including polyketides altered by genetic engineering, but also of increasing the diversity of polyketide libraries by combinatorial attachment of sugars. | 04-23-2009 |
20090286288 | ALPHA-1, 4-GALACTOSYLTRANSFERASE (CgtD) FROM CAMPYLOBACTER JEJUNI - α-1,4-galactosyltransferase (CgtD) polypeptides, nucleic acids that encode the polypeptides, including a polypeptide from | 11-19-2009 |
20100015671 | CYCLIC MALTOSYLMALTOSE, CYCLIC MALTOSYMALTOSE-FORMING ENZYME, THEIR PREPARATION AND USES - An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof. | 01-21-2010 |
20100317065 | GLUCANOTRANSFERASE - The present invention describes an isolated polypeptide which has glucanotransferase activity, selected from the group consisting of: a) a polypeptide which has an amino acid sequence which has at least 40% amino acid sequence identity with amino acids 1 to 555, 1 to 549 or 1 to 567 of SEQ ID NO: 3 or 6 or a fragment thereof; b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO: 1, 2, 4 or 5 which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO: 1, 2, 4 or 5. | 12-16-2010 |
20110014661 | METHOD OF PRODUCING SIALYLATED OLIGOSACCHARIDES - The present invention relates to a method for the large scale in vivo synthesis of sialylated oligosaccharides, culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as lactose, wherein said microorganism comprises heterologous genes encoding a CMP-Neu5Ac synthetase, a sialic acid synthase, a GlcNAc-6-phosphate 2 epimerase and a sialyltransferase, and wherein the endogenous genes coding for sialic acid aldolase (NanA) and for ManNac kinase (NanK) have been deleted or inactivated. The invention also relates to these micoorganisms which are capable of producing internally activated sialic acid. | 01-20-2011 |
20110129879 | Hybrid Genes and Enzymes of Glucanase and Dextransucrase and Processes for Preparing Isomalto-Oligosaccharides or Dextran Using the Same - Disclosed are hybrid genes of glucanase and dextransucrase, recombinant vectors comprising said hybrid genes, microorganisms which are transformed with said recombinant vectors, hybrid enzymes which are expressed from said hybrid genes, and processes for preparing isomalto-oligosaccharides or dextran using said microorganisms or enzymes. Expensive isomalto-oligosaccharides and low molecular weight dextran for clinical use can be produced simply and effectively from cheap substrate-sucrose, using a single bacterial strain or enzyme. | 06-02-2011 |
20110236934 | PRODUCTION OF GLOBOSIDES OLIGOSACCHARIEDS USING METABOLICALLY ENGINEERED MICROORGANISMS - The present invention relates to the large scale in vivo synthesis of globosides oligosaccharides; especially globotriose, globotetraose and globopentaose, which are the carbohydrate portions of globotriosylceramide (Gb3Cer), globotetraosylceramide (Gb4Cer) and globopentaosylceramide (Gb5Cer) respectively. It also relates to high yield production of potential anticancer vaccines of the globo-series glycosphingo lipids, including the Globo-H. It also relates to the use of the glycosyltransferase encoded by the lgtD gene from | 09-29-2011 |
20110300584 | SYNTHESIS OF FUCOSYLATED COMPOUNDS - A method for making a genetically modified cell having the ability to produce fucosylated compounds comprising the steps of
| 12-08-2011 |
20120070863 | NOVEL BETA-GALACTOSIDE-ALPHA2,3-SIALYLTRANSFERASE, A GENE ENCODING THEREOF, AND A METHOD FOR PRODUCING THEREOF - The present invention provides a novel β-galactoside-α2,3-sialyltransferase and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase, as well as a method for producing the sialyltransferase using such a microorganism. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,3-sialyltransferase. The present invention further provides a method for preparing a sialylsugar chain, which uses the sialyltransferase of the present invention. | 03-22-2012 |
20120156732 | USEFUL POLYPEPTIDES - The present invention provides a novel polypeptide having a β1,3-N-acetylglucosaminyltransferase activity; a method for producing the polypeptide; a DNA which encodes the polypeptide; a recombinant vector into which the DNA is inserted; a transformant comprising the recombinant vector; a method for producing a sugar chain or complex carbohydrate, using the polypeptide; a method for producing a sugar chain or complex carbohydrate, using the transformant; an antibody which recognizes the polypeptide; a method for screening a substance which changes the expression of the gene which encodes the polypeptide; and a method for screening a substance which changes the activity of the polypeptide. | 06-21-2012 |
20120282659 | NOVEL BETA-GALACTOSIDE-ALPHA2,6-SIALYLTRANSFERASE, A GENE ENCODING THEREOF, AND A METHOD FOR ENHANCING ENZYME ACTIVITY - The present invention provides an extremely useful and novel β-galactoside-α2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant β-galactoside-α2,6-sialyltransferase. | 11-08-2012 |
20130084607 | Method of Producing a Diutan Gum - The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of | 04-04-2013 |
20130089896 | ALPHA-1, 4-GALACTOSYLTRANSFERASE (CGTD) FROM CAMPYLOBACTER JEJUNI - α-1,4-galactosyltransferase (CgtD) polypeptides, nucleic acids that encode the polypeptides, including a polypeptide from | 04-11-2013 |
20130122553 | METABOLICALLY ENGINEERED ORGANISMS FOR THE PRODUCTION OF ADDED VALUE BIO-PRODUCTS - The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can produce said valuable specialty products in large quantities and at a high rate by bypassing classical technical problems that occur in biocatalytical or fermentative production processes. | 05-16-2013 |
20130157316 | INCREASED POLY (ALPHA 1,3 GLUCAN) YIELD USING BORIC ACID - A process for production of poly(α1,3 glucan) from a renewable feedstock, for applications in fibers, films, and pulps. The effect of addition of boric acid in increasing the yield of the desired end products, poly(α1,3 glucan) and fructose, and decreasing formation of the undesired by-product leucrose. | 06-20-2013 |
20130196384 | INCREASED POLY (ALPHA 1,3 GLUCAN) YIELD USING TETRABORATE - A process for production of poly (α 1,3 glucan) from a renewable feedstock, for applications in fibers, films, and pulps. The effect of addition of tetraborate in increasing the yield of the desired end products, poly (α 1,3 glucan) and fructose, and decreasing formation of the undesired by-product leucrose. | 08-01-2013 |
20130196385 | PmST2 ENZYME FOR CHEMOENZYMATIC SYNTHESIS OF ALPHA-2-3-SIALYLGLYCOLIPIDS - The present invention provides novel methods for preparing glycolipid products. Novel sialyltransferases are also disclosed. | 08-01-2013 |
20130236932 | POLYPEPTIDES WITH PERMEASE ACTIVITY - The invention relates to a polypeptide having a mutation at one or more position corresponding to T219 of SEQ ID NO: 55, wherein the polypeptide has at least 50% sequence identity with SEQ ID NO: 55, and wherein the polypeptide has permease activity. | 09-12-2013 |
20130244287 | HIGH TITER PRODUCTION OF HIGHLY LINEAR POLY (ALPHA 1,3 GLUCAN) - A process for enzymatic preparation of a highly linear poly(α 1, 3 glucan) from sucrose is disclosed. The glucosyltransferase enzyme (gtfJ) from | 09-19-2013 |
20130244288 | HIGH TITER PRODUCTION OF POLY (ALPHA 1,3 GLUCAN) - A process for enzymatic preparation of poly (α1, 3 glucan) from sucrose is disclosed. The glucosyltransferase enzyme (gtfJ) from | 09-19-2013 |
20130295618 | PROCESS FOR PRODUCING A PARTICULATE COMPOSITION COMPRISING ANHYDROUS CRYSTALLINE 2-O-ALPHA-D-GLUCOSYL-L-ASCORBIC ACID - The invention provides a process for enabling the production of a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside that does not significantly cake even when the production yield of ascorbic acid 2-glucoside does not reach 35% by weight. The process for producing a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside, which comprises allowing a CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid and then allowing a glucoamylase to act on the resulting solution to obtain a solution with an ascorbic acid 2-glucoside production yield of at least 27%, purifying the obtained solution to increase the ascorbic acid 2-glucoside content to a level of over 86% by weight, precipitating anhydrous crystalline ascorbic acid 2-glucoside by a controlled cooling method or pseudo-controlled cooling method, collecting the precipitated anhydrous crystalline ascorbic acid 2-glucoside, and ageing and drying the collected anhydrous crystalline ascorbic acid 2-glucoside. | 11-07-2013 |
20130302857 | TREHALOSE PHOSPHORYLASES AND THEIR USE IN THE BIOCATALYTIC PRODUCTION OF TREHALOSE-ANALOGUES AND GLYCOSYL PHOSPHATES - The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogues and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism | 11-14-2013 |
20130316406 | THERMOPHILIC AND THERMOACIDOPHILIC BIOPOLYMER-DEGRADING GENES AND ENZYMES FROM ALICYCLOBACILLUS ACIDOCALDARIUS AND RELATED ORGANISMS, METHODS - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 11-28-2013 |
20130330780 | Fusion Enzymes - The present disclosure relates to recombinant proteins having N-acetylglucosaminyltransferase activity. The present disclosure further relates to methods for producing complex N-glycans including the steps of providing host cells containing such recombinant proteins and culturing the host cells such that the recombinant proteins are expressed. | 12-12-2013 |
20140024082 | Activated Sugars - Kinase and nucleotidyltransferase enzymes for the production of activated sugars have been developed. These enzymes have improved stability for industrial application and relaxed specificity towards a variety of sugars. These enzymes are useful in, for example, the production of diverse NDP-sugars for glycosylation of aglycones of interest, production of oligosaccharides, production of other important glycosylated sugars, and in drug discovery applications. | 01-23-2014 |
20140051127 | LARGE SCALE ENZYMATIC SYNTHESIS OF OLIGOSACCHARIDES - A novel UDP-Gal regeneration process and its combined use with a galactosyltransferase to add galactose to a suitable acceptor substrate. Also described herein are synthetic methods for generating Globo-series oligosaccharides in large scale, wherein the methods may involve the combination of a glycosyltransferase reaction and a nucleotide sugar regeneration process. | 02-20-2014 |
20140057323 | ENHANCED CELLODEXTRIN METABOLISM - The present disclosure relates to host cells containing two or more of a recombinant cellodextrin transporter, a recombinant cellodextrin phosphorylase, a recombinant β-glucosidase, a recombinant phosphoglucomutase, or a recombinant hexokinase; and to methods of using such cells for degrading cellodextrin, for producing hydrocarbons or hydrocarbon derivatives from cellodextrin, and for reducing ATP consumption during glucose utilization. | 02-27-2014 |
20140087431 | GLUCOSYLTRANSFERASE ENZYMES FOR PRODUCTION OF GLUCAN POLYMERS - Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan. | 03-27-2014 |
20140134676 | BRANCHED ALPHA-GLUCAN, ALPHA-GLUCOSYLTRANSFERASE WHICH FORMS THE GLUCAN, THEIR PREPARATION AND USES - The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched α-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows:
| 05-15-2014 |
20140178943 | PROCESS FOR PRODUCING A PARTICULATE COMPOSITION COMPRISING ANHYDROUS CRYSTALLINE 2-O-ALPHA-D-GLUCOSYL-L-ASCORBIC ACID - The invention provides a process for enabling the production of a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside that does not significantly cake even when the production yield of ascorbic acid 2-glucoside does not reach 35% by weight. The process for producing a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside, which comprises allowing a CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid and then allowing a glucoamylase to act on the resulting solution to obtain a solution with an ascorbic acid 2-glucoside production yield of at least 27%, purifying the obtained solution to increase the ascorbic acid 2-glucoside content to a level of over 86% by weight, precipitating anhydrous crystalline ascorbic acid 2-glucoside by a controlled cooling method or pseudo-controlled cooling method, collecting the precipitated anhydrous crystalline ascorbic acid 2-glucoside, and ageing and drying the collected anhydrous crystalline ascorbic acid 2-glucoside. | 06-26-2014 |
20140199733 | Method for producing gamma-cyclodextrin by simultaneous use of gamma-cyclodextrin glycosyltransferase and isoamylase - The present invention provides a method for the production of γ-CD, comprising making a starch slurry, incubating with γ-CGTase and isoamylase simultaneously for γ-CD production, forming a complex of γ-CD and an organic complexant, and purifying γ-CD from the complexant. The present invention provides a simple and cost-effective method for producing high purity γ-CD, which has a short production cycle, a high conversion rate, and is adaptable to large-scale industrial production. | 07-17-2014 |
20140273103 | POLYSIALIC ACID, BLOOD GROUP ANTIGENS AND GLYCOPROTEIN EXPRESSION IN PROKARYOTES - The invention described herein generally relates to glycoengineering host cells for the production of glycoproteins for therapeutic use. Host cells are modified to express biosynthetic glycosylation pathways. Novel prokaryotic host cells are engineered to produce N-linked glycoproteins wherein the glycoproteins comprise polysialic acid or blood group antigens. | 09-18-2014 |
20140302565 | PMST1 MUTANTS FOR CHEMOENZYMATIC SYNTHESIS OF SIALYL LEWIS X COMPOUNDS - The present invention provides mutants of PmST1 for the preparation of sialyl-Lewis | 10-09-2014 |
20150010954 | GLUCOSYLTRANSFERASE ENZYMES FOR PRODUCTION OF GLUCAN POLYMERS - Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan. | 01-08-2015 |
20150010955 | GLUCOSYLTRANSFERASE ENZYMES FOR PRODUCTION OF GLUCAN POLYMERS - Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan. | 01-08-2015 |
20150010956 | GLUCOSYLTRANSFERASE ENZYMES FOR PRODUCTION OF GLUCAN POLYMERS - Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan. | 01-08-2015 |
20150064748 | PROCESS FOR PRODUCING APLHA-1,3-GLUCAN POLYMER WITH REDUCED MOLECULAR WEIGHT - A process for producing poly alpha-1,3-glucan with reduced molecular weight is disclosed. The process comprises contacting water, sucrose, a polar organic solvent, and a glucosyltransferase enzyme in a solution to produce poly alpha-1,3-glucan. This contacting step results in the production of poly alpha-1,3-glucan having a reduced molecular weight compared to the molecular weight of a poly alpha-1,3-glucan made in the absence of the polar organic solvent. | 03-05-2015 |
20160053290 | Production of Defined Monodisperse Heparosan Polymers and Unnatural Polymers with Polysaccharide Synthases - A methodology for polymer grafting by a polysaccharide synthase allows the creation of a variety of glycosaminoglycan oligosaccharides that may have a natural, chimeric, hybrid, and/or unnatural sugar structure and/or a targeted size (i.e., substantially monodisperse in size). | 02-25-2016 |
20160194676 | GENETICALLY MODIFIED MICROORGANISMS CAPABLE OF PRODUCING BETA-GLUCANS AND METHODS FOR PRODUCING BETA-GLUCANS | 07-07-2016 |