| Entries |
| Document | Title | Date |
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| 20080213820 | Method for Determining the Selectivity of a Pre-Synaptic Neuromuscular Blocking Substance - The invention relates to a method for determining the quantity of pre-synaptic neuromuscular blocking substance (notably botulinum toxin) contained in a sample. In one aspects, the method comprises the following steps:
| 09-04-2008 |
| 20080220465 | Antibiotic Sensitivity Testing Method - The method includes several steps including obtaining a bacterial sample; identifying the type of bacteria in the bacterial sample; selecting a set of antibiotics based on the identity of the bacteria in the bacterial sample; obtaining a control sample from the bacterial sample; placing the bacterial sample in solutions containing the set of antibiotics; determining concentration of bacteria in the respective antibiotic solutions; determining growth curves for the respective antibiotic solutions based on the determined bacterial concentration; and comparing the growth curves for the respective antibiotic solutions with a growth curve determined from the control sample. An identification and quantification system may be used to select the set of antibiotics, and further may be used in the steps of determining concentration of bacteria in the respective antibiotic solutions and determining growth curves for the respective antibiotic solutions based on the determined bacterial concentration. | 09-11-2008 |
| 20080227141 | PROCESS FOR PRODUCING ISOPRENOID COMPOUNDS BY MICROORGANISMS AND A METHOD FOR SCREENING COMPOUNDS WITH ANTIBIOTIC OR WEEDING ACTIVITY - The present invention provides a process for producing isoprenoid compounds or proteins encoded by DNA using DNA that contains one or more of the DNA encoding proteins having activity to improve efficiency in the biosynthesis of isoprenoid compounds effective in pharmaceuticals for cardiac diseases, osteoporosis, homeostasis, prevention of cancer, and immunopotentiation, health food and anti-fouling paint products against barnacles; the DNA; the protein; and a method for screening a substance with antibiotic and weeding activities comprising screening a substance inhibiting enzymatic reaction on the non-mevalonate pathway. | 09-18-2008 |
| 20080254501 | CHEMICAL PROBE COMPOUNDS THAT BECOME FLUORESCENT UPON REDUCTION, AND METHODS FOR THEIR USE - Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents. | 10-16-2008 |
| 20080261263 | In Vitro Evaluation of Micro-Organisms and Their Antimicrobial Agent Susceptibilities - A method of identifying a micro-organism and determining the susceptibility of the micro-organism to an antimicrobial agent by inserting a sample of the micro-organism in a primary culture container and allowing the micro-organism to multiply. The primary culture is then divided into a plurality of secondary cultures and the metabolic volatile or semi-volatile compounds (VCs) in the headspace above the cultures are analysed by SIFT-MS to ascertain whether micro-organisms exist in the culture and determine the type of micro-organism. The secondary cultures are then divided into a number of tertiary cultures and an antimicrobial agent is introduced whereupon the VCs in the headspace above the tertiary cultures are analysed by SIFT-MS to determine the susceptibility of the micro-organism to the antimicrobial agent at various concentrations of the antimicrobial agent. | 10-23-2008 |
| 20080293093 | Determining presence of antibiotic in a fluid - A test system for the determination of the presence of an antibiotic in a fluid using Bromthymol Blue as an indicator. | 11-27-2008 |
| 20080318267 | Methods and Compositions for Ultra-High Throughput Screening of Natural Products - The present invention provides cells having more than two drug resistance genes and at least two different resistance genes that have been recombined into the chromosome of a cell. It also teaches the processes for preparing cells by recombining two or more different drug resistance genes into the chromosome of a cell. The invention further shows a screening method using the cells of described herein that may be used to accomplish high throughput screening of, among other things, natural products and/or whole cells isolated from the environment. | 12-25-2008 |
| 20090004689 | Lineage Restricted Glial Precursors from the Central Nervous System - A glial precursor cell population from mammalian central nervous system has been isolated. These A2B5 | 01-01-2009 |
| 20090011457 | Method and device for testing the bactericidal effect of substances - The method for checking the degree of microbial loading of laundry after a wash cycle is characterized in that a defined amount of living microorganisms are washed together with the laundry, in that the number of microorganisms still living after the end of the wash process is determined and in that the effectiveness or quality of the wash process is determined from the difference between the original amount of living microorganisms and the amount of microorganisms which still remain alive after the wash process. A device for carrying out this method comprises inter alia a container ( | 01-08-2009 |
| 20090017489 | Methods For Modulating The Development Of Dopamine Neuron By The Dopamine D2 Receptor And Compositions Thereof - The present invention relates to a composition for modulating the activation of Nurr1, the composition comprising an agonist or an antagonist of a dopamine D2 receptor, methods for modulating the activation of Nurr1 by the dopamine D2 receptor, a method and composition for treating Nurr1-related diseases using the dopamine D2 receptor, and methods for screening a modulator of a dopamine D2 receptor of a test compound. Accordingly, the activation of Nurr1 can be modulated by treating the dopaminergic neurons with the agonist or the antagonist of the dopamine D2 receptor, thereby enhancing or inhibiting generation of the dopaminergic neurons. | 01-15-2009 |
| 20090042240 | RAPID DETECTION OF ANTIMICROBIAL DRUG RESIDUES - The present invention relates to a novel method for the rapid detection of the presence or absence of antimicrobial drug residues belonging to the class of the quinolones in liquid samples such as milk, meat juice, serum, urine, blood, eggs or extracts obtained from animal tissues or food products. | 02-12-2009 |
| 20090053758 | Processes for clonal growth of hepatic progenitor cells - A method of propagating mammalian endodermally derived progenitors such as hepatic progenitors, their progeny, or mixtures thereof is developed which includes culturing mammalian progenitors, their progeny, or mixtures thereof on a layer of embryonic mammalian feeder cells in a culture medium. The culture medium can be supplemented with one or more hormones and other growth agents. These hormones and other growth agents can include insulin, dexamethasone, transferrin, nicotinamide, serum albumin, β-mercaptoethanol, free fatty acid, glutamine, CuSO | 02-26-2009 |
| 20090104651 | SCREENING PROCESS FOR ANTIBACTERIAL AGENTS - The present invention relates to a method of preparing a phenotypically antibiotic-resistant subpopulation of stationary phase bacteria by treating stationary phase bacteria with high doses of antibacterial agents, the subpopulation thus identified, a process for identifying new antibacterial agents by testing against the antibiotic-resistant subpopulation, the compounds thus identified and their uses, particularly in treating bacterial infections involving dormant bacteria. | 04-23-2009 |
| 20090142796 | Detection of Inducible Resistance to Macrolide-Lincosamide-Streptogramin b - The embodiments of this invention provide a test panel and method for the detection of inducible resistance to macrolide-lincosamide-streptogramin b (iMLSb) in microorganisms in an automated microorganism identification (ID) and antimicrobial susceptibility determinations (AST) system. | 06-04-2009 |
| 20090170150 | Screening Assay for Ribosomal Antibiotics - The present invention is directed to a method for identifying ribosomal antimicrobial substances being selective for microbial but not for mitochondrial and/or cytosolic ribosomes. Specifically, said method is directed to an assay that compares the interaction of a candidate ribosomal antimicrobial substance (i) in a bacterial strain with microbial ribosomes, and (ii) in a bacterial strain with chimeric mitochondrial bacterial ribosomes, and/or (iii) in a bacterial strain with chimeric cytosolic bacterial ribosomes. In a further aspect the present invention also relates to the use of bacterial strains with microbial ribosomes, and bacterial strains with chimeric mitochondrial bacterial ribosomes, and/or bacterial strains with chimeric cytosolic bacterial ribosomes for identifying ribosomal antimicrobial substance being selective for microbial but not for mitochondrial and/or cytosolic ribosomes. Furthermore, one or more of the above bacterial strains (i) to (iii) may be substituted by a functionally equivalent cell-free biological system. | 07-02-2009 |
| 20090258384 | Direct Antimicrobial Susceptibility Assay - An antimicrobial susceptibility assay, including the steps of: providing an assay dish; providing a growth medium in said dish; providing an antimicrobial agent sample; providing an interpretive indication located a predetermined distance from a sample location adjacent the growth medium in the assay dish; providing an interfitting element which interfits with said dish, said interfitting element configured to enable at least one the steps of: a) more accurately positioning the antimicrobial sample at the sample location in contact with the growth medium; b) providing the interpretive indication at a predetermined distance from said sample position to enable said interpretative indication to be compared with a margin of a zone of inhibition of a colony grown on said medium to determine an assay result, wherein said result can include determination of at least one of: a) “susceptible;” “intermediate;” and, “resistant;” placing a microorganism on the growth medium; placing the antimicrobial agent sample at said sample location with accuracy using said interfitting element; incubating said microorganism for a period sufficient to allow a margin of a zone of inhibition to be discernable; and, comparing the location of the margin of the zone of inhibition to the interpretive indication, to obtain a result including a determination of at least one of a) “susceptible;” b) “intermediate;” and, c) “resistant” for said microorganism with respect to the antimicrobial agent. | 10-15-2009 |
| 20100062481 | Methods of Assaying Sensitivity of Cancer Stem Cells to Therapeutic Modalities - The present invention is directed to methods of measuring the proliferative ability of individual patient cancer stem cells. The present invention provides a method for treating a cancer patient according to an assay of the individual patient's tumor's cancer stem cell sensitivity, by measuring the proliferative ability of cancer stem cells from the patient. By the methods of the present invention it is possible to treat individual cancer stem cells presented in tumor cells. Methods of detecting and enumerating cancer stem cells in hybrid spheroids comprised of fibroblasts and tumor cells are also provided by the present invention. The present invention also contemplates a method for drug and other treatment development, wherein the effects of a drug or combination of drugs or other treatments are determined on the individual patient's cancer stem cells. | 03-11-2010 |
| 20100068753 | Biotin-facilitated transport in gram negative bacteria - Biotinylation of compounds such as peptides and peptidomimetics facilitates illicit transport of the compounds into Gram negative bacteria. | 03-18-2010 |
| 20100099137 | Direct Antimicrobial Susceptibility Assay - An antimicrobial susceptibility assay, including an assay dish including at least one chamber, at least one growth medium carried by the assay dish, at least one antimicrobial agent sample positionable at a sample location adjacent a growth medium in the assay dish, at least one interpretive indication positionable at a predetermined distance from the sample location adjacent a growth medium in the assay dish, and at least one interfitting element, said at least one interfitting element configured cooperate with the assay dish to enable at least one of: a) more accurately positioning said at least one antimicrobial sample at the sample position in contact with the growth medium; b) carrying said at least one interpretive indication positionable at a predetermined distance from said sample position; said assay enabling the interpretative indication to be compared with a margin of a zone of inhibition of microbial organism growth on said growth medium to determine an assay result, wherein said result enables determination of at least one of: a) “susceptible;” “intermediate;” and, “resistant.” | 04-22-2010 |
| 20100099138 | MULTIPLE-VALENT OPSONOPHAGOCYTIC ASSAY SELECTION PANEL ARRAYS AND USES THEREFOR - This application discloses a multivalent opsonophagocytosis assay that does not rely on counting of bacterial colonies to determine bacteria viability following opsonophagocytosis. Instead, the method uses a metabolic colorimetric indicator to determine if viable bacteria are present. Also disclosed are arrays that can be used to determine the viability of bacteria following opsonophagocytosis. | 04-22-2010 |
| 20100105103 | ALCOHOL TOLERANT ESCHERICHIA COLI AND METHODS OF PREPARATION THEREOF - The present invention relates to | 04-29-2010 |
| 20100311107 | Ultra-High Throughput Screening of Natural Products - The present invention provides cells having more than two drug resistance genes and at least two different resistance genes that have been recombined into the chromosome of a cell. It also teaches the processes for preparing cells by recombining two or more different drug resistance genes into the chromosome of a cell. The invention further shows a screening method using the cells of described herein that may be used to accomplish high throughput screening of, among other things, natural products and/or whole cells isolated from the environment. | 12-09-2010 |
| 20110008823 | TOXICITY SCREENING METHODS - The present invention provides methods of determining a level of toxicity of a given compound based on in vitro assays. The present invention provides particular methods of determining organ-specific toxicity and species-specific toxicity of a given compound based on in vitro assays. In addition, the present invention provides methods of determining a level of toxicity in normal tissue for an anti-tumor compound. The methods include providing at least one cell type and culturing the cell type in the presence of at least one concentration of the chemical compound, measuring at least one indicator of cell health at the at least one concentration of compound for the at least one cell type, and performing a concentration response analysis, from which a toxic concentration can be determined. | 01-13-2011 |
| 20110014644 | METHODS FOR PREDICTING A CANCER PATIENT'S RESPONSE TO ANTIFOLATE CHEMOTHERAPY - The present invention provides methods for individualizing therapy for cancer treatment, and particularly for evaluating a patient's responsiveness to one or more antifolate therapeutic agents prior to treatment with such agents. Particularly, the invention provides an in vitro chemoresponse assay for predicting a patient's response to an antifolate agent, such as pemetrexed or methotrexate. | 01-20-2011 |
| 20110143390 | METHOD FOR TESTING DRUG SENSITIVITY AND DEVICE USED THEREFOR - A method for testing drug sensitivity including: a) concentrating a bacterium or cell sample to be tested; b) adding a culture solution to the bacterium or cell sample to yield a liquid sample, the culture solution comprising a nutrient and a colloidal material; c) adding a coagulant aid to the liquid sample where the coagulant aid reacts with the colloidal material to yield a gel test sample; d) pasting drug paper on the surface of the gel test sample; e) culturing the gel test sample in an incubator; and f) observing the formation of a drug inhibition zone and determining the drug sensitivity of the bacterium or cell. A device used for testing drug sensitivity according to the method is also provided. The method and the device have short testing time, low cost, and safe operation. | 06-16-2011 |
| 20110165613 | STABLE CLONE CELL EXPRESSING A PRION - The invention relates to a cell clone derived from the MovS6 line, said cell clone expressing a prion protein PrP and being capable of tolerating the replication or propagation of the pathological form PrPsc of said PrP, characterized in that its titre with respect to marker for infection with a non-conventional transmissible agent (NCTA) is stable at least up to the 6 | 07-07-2011 |
| 20110189726 | BIOSYNTHESIS OF DERIVATIVES OF MONACOLIN J - The invention relates to a process for obtaining monacolin J derivatives (I), wherein R | 08-04-2011 |
| 20110195447 | Identification of Non-Small Cell Lung Carcinoma (NSCLC) Tumors Expressing PDGFR-ALPHA - The invention discloses a previously unidentified subset of mammalian non-small cell lung carcinomas (NSCLC) in which platelet-derived growth factor receptor alpha (PDGFRα) is expressed and is driving the disease, and provides methods for identifying a mammalian NSCLC tumor that belongs to a subset of NSCLC tumors in which PDGFRα is expressed, and for identifying a NSCLC tumor that is likely to respond to a PDGFRα-inhibiting therapeutic. The invention also provides methods for inhibiting the progression of a mammalian NSCLC tumor in which PDGFRα is expressed, and for determining whether a compound inhibits the progression of a PDGFRα-expressing mammalian NSCLC tumor. | 08-11-2011 |
| 20110207166 | Human bone marrow microenvironments and uses thereof - The present invention is directed to an in vitro cultured permissive niche, or human bone marrow microenvironment, comprising a scaffold coated with human mesenchymal stem cells and a culture medium, wherein the stem cells are viable and proliferate in culture and the niche is permissive for the establishment of introduced hematopoietic or leukemic cell populations. The present invention is also directed to establishment of a permissive niche in a non-human animal model comprising a scaffold coated with human mesenchymal stem cells introduced into the animal ectopically, wherein the niche and the model are permissive for the establishment of introduced hematopoietic or leukemic cell populations. The implanted scaffold forms an ectopic human bone marrow microenvironment to study the mesenchymal leukemic stem cell niche. In addition, the present invention is directed to methods of using the in vitro cultured human bone marrow microenvironment and the non-human animal model to evaluate an agent for anti-leukemic properties. | 08-25-2011 |
| 20110217728 | HIGH THROUGHPUT TEST METHOD FOR EVALUATION OF BIOCIDES AGAINST ANAEROBIC MICROORGANISMS - Provided is a high-throughput method for determining the biocidal efficacy of biocidal agents against anaerobic organisms. The method includes the steps of: providing one or more anaerobe samples in a first set of multiple receptacles accessible to multi-channel pipettes; providing one or more biocidal samples at known concentration(s) in a second set of multiple receptacles accessible to multi-channel pipettes; forming mixtures of the one or more biocidal samples and the anaerobe samples via a multi-channel pipette; incubating the mixtures so as to allow reaction between the biocidal samples and the anaerobe samples; determining each of the one or more biocidal samples' killing (biocidal) effectiveness against the anaerobes at selected time interval(s), wherein each step, except for the step of providing one or more biocidal samples, is conducted under anaerobic conditions. | 09-08-2011 |
| 20110269176 | METHODS FOR ASSAYING RESPONSES TO VACCINES - The present invention incorporates germinal centers (GCs) into three-dimensional (3D) engineered tissue constructs (ETCs). In an embodiment, we have incorporated the GC in the design of an artificial immune system (AIS) to examine immune responses to vaccines and other compounds. Development of an in vitro GC adds functionality to an AIS, in that it enables generation of an in vitro human humoral response by human B lymphocytes that is accurate and reproducible, without using human subjects. The invention also permits evaluation of, for example, vaccines, allergens, and immunogens, and activation of human B cells specific for a given antigen, which can then be used to generate human antibodies. In an embodiment of the present invention the function of the in vitro GC is enhanced by placing FDCs and other immune cells in a 3D ETC; FDCs appear more effective over a longer time (antibody production is sustained for up to about 14 days. | 11-03-2011 |
| 20110312019 | Predicting Human Developmental Toxicity of Pharmaceuticals Using Human Stem-Like Cells and Metabolomics - The invention provides biomarker profiles of metabolites and methods for screening chemical compounds including pharmaceutical agents, lead and candidate drug compounds and other chemicals using human stem-like cells (hSLCs) or lineage-specific cells produced therefrom. The inventive methods are useful for testing toxicity, particularly developmental toxicity and detecting teratogenic effects of such chemical compounds. Specifically, a more predictive developmental toxicity model, based on an in vitro method that utilizes both hSLCs and metabolomics to discover biomarkers of developmental toxicity is disclosed. | 12-22-2011 |
| 20110312020 | Ex-Vivo Passive Protection Bacteremia Assay - The present disclosure provides methods for assessing bactericidal antibodies in a biological sample by use of human fresh whole blood from a non-immune human as a reaction medium for the assay. | 12-22-2011 |
| 20110318775 | METHOD, SYSTEM AND COMPOSITION FOR OPTICALLY INDUCING CARDIOMYOCYTE CONTRACTION - The present invention provides a method, system and composition for screening drug candidates for cardiotoxicity and for novel drugs that effect cardiomyocyte contractility and function. The invention provides an efficient and reliable screening assay to detect the effect of new and potential drug candidates on cardiomyocyte calcium flux, membrane depolarization, and/or the propagation of action potentials. | 12-29-2011 |
| 20120015397 | Periodontal Disease Marker (as amended herewith) - A marker for determining the onset of periodontal disease and a marker for determining the progression stage of periodontal disease, each containing autoinducer-2. | 01-19-2012 |
| 20120040394 | MICROTUMOURS - A method of producing an in vitro microtumour comprising: seeding a colorectal neoplastic cell into a three dimensional scaffold comprising polysaccharide co-polymer; providing said cell with a culture medium that supports the growth thereof; and incubating said cell in said scaffold for a time sufficient for microtumors to form, wherein said polysaccharide copolymer comprises glutaronate and mannuronate. | 02-16-2012 |
| 20120058507 | Clonal Derivation and Cell Culture quality Control Screening Methods - The invention provides methods of label-free detection of changes in cell populations and mixed cell populations. | 03-08-2012 |
| 20120094323 | CARDIOMYOCYTES-CONTAINING DEVICE AND METHOD FOR MANUFACTURING AND USING THE SAME - Disclosed is a device for determining the cardiotoxicity of a chemical compound, comprising a substrate ( | 04-19-2012 |
| 20120107863 | MODULATION OF TELOMERE LENGTH IN TELOMERASE POSITIVE CELLS AND CANCER THERAPY - Induction of telomere shortening, G2 arrest and apoptosis in telomerase positive cancer cells using acyclic nucleoside analogs has been disclosed. In addition, methods for impairment or prevention of tumorigenic telomerase positive cells from having a chance to grow into a tumor and methods for promoting tumor regression (decrease in size of an established tumor) using acyclic nucleoside analogs has been disclosed. | 05-03-2012 |
| 20120122147 | REAL-TIME METHOD FOR THE DETECTION OF VIABLE MICRO-ORGANISMS - The invention relates to a method for real-time detection of viable microorganisms comprising: a. addition of a cell-permeable, phototautomeric compound to a micro-organism or other living cell; and b. measuring the fluorescent emission of said phototautomeric compound. Preferably the phototautomeric compound is salicylic acid, 2-hydroxy-1-naphtoic acid or 1-hydroxy-2-naphtoic acid. Further, the assay can he used to assess the antibiotic effect of a test compound. This test can be used as a high—throughput screening for compounds with antibiotic activity. Also part of the invention is the use of a cell permeable phototautomeric compound in a method for determining the viability of micro-organisms and for assessing the antibiotic effect of a test compound. | 05-17-2012 |
| 20120122148 | Media For The Specific Detection Of Gram-Negative Bacteria Resistant To Beta-Lactam Antibiotics - The invention relates to a reaction medium for gram-negative bacteria having a beta-lactam antibiotic resistance mechanism, comprising:
| 05-17-2012 |
| 20120135453 | Methods of Treatment Using CCA1 Inhibitors - Provided herein are methods for the treatment or prevention of a fungal infection in a host comprising the administration to the host a therapeutically or prophylactically effective amount of a CCA1 inhibitor. | 05-31-2012 |
| 20120149055 | PAPER STRIP FOR DETERMINING MINIMUM INHIBITORY CONCENTRATIONS OF ANTIBIOTICS - The present invention concerns the sector of chemical, clinical, bacteriological and immunological analyses and more specifically it concerns the use of a strip of impregnated paper in order to determine, in the simplest, most economical and most accurate way, the correct Minimum Inhibitory Concentration (M.I.C.) of antibiotic molecules to be administered to patients as claimed in patent No. EP 0 157 071. | 06-14-2012 |
| 20120156715 | Environmental Sentinel Biomonitor System (ESB) - An embodiment of the invention provides a system for toxicity identification in water. The system includes a cell maintenance device having a cooling component that maintains an internal storage area of the cell maintenance device within a temperature range of 4 degrees Celsius to 25 degrees Celsius. Fluidic biochips in the cell maintenance device include one or more testing components for receiving living poikilothermic cells and at least one water test sample. A test unit receives the fluidic biochips and monitors a response to exposure of the poikilothermic cells to the water test sample. In at least one embodiment, the test unit is able to identify the degree of toxicity of the water sample within 60 minutes of receiving the fluidic biochip. | 06-21-2012 |
| 20120164680 | Asynchronous Magnetic Bead Rotation Sensing Systems and Methods - Described herein are various methods, devices and systems for performing asynchronous magnetic bead rotation (AMBR) to detect and monitor cellular growth and/or behavior. Cluster rotation of magnetic particles for AMBR is descried. In particular, described herein are systems for the parallel analysis of multiple wells of a sample plate. Also described herein are methods for controlling the illumination and imaging of rotating magnetic particles. | 06-28-2012 |
| 20120183991 | METHOD FOR TESTING DRUG SENSITIVITY OF MYCOBACTERIUM TUBERCULOSIS, APPLICATION OF INDICATOR, AND SOLID MEDIUM - A method for testing of drug sensitivity of | 07-19-2012 |
| 20120219985 | METHOD FOR THE TOXICITY ASSESSMENTS OF NANO-MATERIALS - The present invention relates to a method for the toxicity assessment of nano-materials, and more specifically, it is relates to an objective, reproducible and accurate assessment method for the unbiased toxicity testings of nano-materials, which minimize artifacts of the conventional methods for the toxicity assessment of the nano-materials by considering the dose characteristics of the nano-material itself using Selective multi-Plane Illumination Microcopy (SPIM); and the response characteristics of the nano-material using the improved or novel cellular responses assessment methods for nano-materials (e.g., modified MTT assay using image cytometric analysis, normal-inverted exposure apparatus, and modified flow cytometry), and a system and an apparatus thereof. | 08-30-2012 |
| 20120231491 | METHOD FOR EVALUATING TOXICITY OF CHEMICAL USING ALGA - The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value. | 09-13-2012 |
| 20120264162 | MASS SPECTROMETRIC MEASUREMENT OF MICROBIAL RESISTANCES - Microorganisms, particularly bacteria, are identified and characterized on the basis of a mass spectrometric measurement of their protein profiles with ionization by matrix-assisted laser desorption. In order to measure the microbial resistance to antibiotics, the protein profiles of microorganisms are measured after cultivation for a short time duration in nutrient media containing the antibiotics. | 10-18-2012 |
| 20120276577 | Method for Determining the Susceptibility of a Cell Strain to Drugs - The present invention relates to a method for determining the susceptibility of a cell strain to a compound intended for controlling the growth of said cell strain, comprising:
| 11-01-2012 |
| 20120322097 | DEVICE AND METHOD FOR CULTURING CELLS IN A BIOMIMETIC ENVIRONMENT - Devices and methods are disclosed herein for a biomimetic flow apparatus. The biomimetic flow apparatus includes a microfluidic flow channel and an inlet in fluid connection with the flow channel for allowing fluid to flow into the flow channel. The flow channel has at least one surface with a topography formed therein. The topography of the at least one surface of the flow channel is selected to cause cells in a cell layer above the surface to achieve an arrangement, behavior, or morphology. The cell arrangement, behavior, or morphology is determined at least in part by the topography of the at least one surface. | 12-20-2012 |
| 20120329086 | CHEMO-SENSITIVITY ASSAYS USING TUMOR CELLS EXHIBITING PERSISTENT PHENOTYPIC CHARACTERISTICS - The assays, methods, tools and systems discussed herein represent an improved and unified system for monitoring the progression of an individual patient malignancy. The assays, methods, tools and systems discussed herein represent an improved and unified system for monitoring and for identifying cellular and secreted markers, for screening cells to detect phenotypic and genotypic drift and for predicting chemotherapeutic response of patient tumor cells to at least one therapeutic agent. The assays, methods, tools and systems discussed herein also represent an improved and unified system for monitoring and for screening multiple pharmaceutical agents for efficacy and long term effect as to a specific patient. | 12-27-2012 |
| 20130040336 | MUTANT STRAINS OF ESCHERICHIA COLI, A METHOD OF TESTING POTENTIAL ANTIBACTERIAL AGENTS USING SAID STRAINS AS WELL AS A TESTING KIT - The subject of the present invention are mutant strains of | 02-14-2013 |
| 20130059327 | Automatic Toxicological Fluid Sample Preparation Module, Automatic Analysis System and Method for Use Thereof - This present invention relates to an automatic toxicological fluid sample preparation module and method for use thereof, for automatically preparing a fluid sample for the detection and measurement of toxics and/or toxicological effects. The invention further relates to an automatic analysis system. The automatic toxicological fluid sample preparation module comprising: an inlet for automatically obtaining a sample directly from a fluid or fluid stream to be analyzed; preparation for preparing the fluid sample from the sample, comprising first coupling means for coupling with the inlet; and—an outlet for discharge of prepared fluid to measurement means, wherein the preparation means comprise indicator micro-organisms. | 03-07-2013 |
| 20130071874 | SYSTEM AND METHOD FOR ANTI-CANCER DRUG CANDIDATE EVALUATION - The disclosure provides a method of evaluating the ability of an anti-cancer drug candidate to induce apoptosis in a known cancer cell line by placing a single-cell suspension of a known cancer cell line in a well of a plate, adding at least one drug candidate to the well in an amount sufficient to achieve a target drug candidate concentration, measuring the optical density at selected time intervals for a selected duration of time, determining a kinetic units value from the optical density and time measurements, and correlating the kinetic units value with an ability of the anti-cancer drug candidate to induce apoptosis in the cancer cell line if the kinetic units value is positive. A similar method may be used to evaluate the ability of an anti-cancer drug candidate to induce apoptosis in a cancer type. | 03-21-2013 |
| 20130084596 | METHODS OF IDENTIFYING LEAD COMPOUNDS THAT MODULATE TOXICITY OF NEUROTOXIC POLYPEPTIDES - Methods of screening candidate agents to identify lead compounds for the development of therapeutic agents for the treatment of a neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease and methods for identifying a mutation in, or changes in expression of, a gene associated with neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease, are provided. | 04-04-2013 |
| 20130109049 | Method of Establishing a Fungal Nail Infection | 05-02-2013 |
| 20130130303 | METHODS FOR SCREENING MICROBIAL REMEDIATION AGENTS - Disclosed are methods for determining the efficacy of antimicrobial agents used in the treatment of building materials after microbial contamination has occurred, for the purpose of killing existing microbial growth and reducing or inhibiting recurrent or subsequent microbial growth. The disclosed methods may be used to determine microbial growth at time points subsequent to antimicrobial treatment of the material surface. The disclosed invention also measures visible microbial growth in a semi-quantitatively analysis. In addition, the Inventors disclosure a method with reduced variability and a more accurate assessment of antimicrobial efficacy. | 05-23-2013 |
| 20130130304 | METHODS FOR SCREENING MICROBIAL GROWTH INHIBITION ACTIVITY ON MATERIALS - Disclosed are methods of determining the effectiveness of an antimicrobial agent in reducing or inhibiting microbial growth on a substrate. The disclosed methods may be used to determine microbial growth at time points subsequent to antimicrobial treatment of the material surface and exposure to microorganisms. The disclosed invention also measures visible microbial growth using a semi-quantitatively method that is more accurate and less subjective than estimates of growth used previously. The disclosed method that provides an accurate assessment of antimicrobial efficacy with reduced variability. | 05-23-2013 |
| 20130130305 | INDENTIFYING ANTIFUNGAL AGENTS THAT INHIBIT IAA OR A YAP FAMILY - The present invention relates to methods of screening for antifungal agents by identifying agents that bind to or otherwise inhibit indole-3-acetic acid (IAA) or that bind to or otherwise inhibit the expression or activity of a protein within the Yap family or a gene encoding a protein within the Yap family. | 05-23-2013 |
| 20130157304 | METHOD FOR PREPARING HUMAN NEOPLASTICALLY TRANSFORMED CELLS - A method for preparing neoplastically transformed cells from human-derived cells, including the step of introducing human telomerase catalytic subunit (hTERT) gene, SV40 small T antigen (SV40ST) gene, and an antisense oligonucleotide derived from human 28S rRNA into the human-derived cells. The method for preparing neoplastically transformed cells from human-derived cells can be utilized when a variety of human normal cells are induced to be neoplastically transformed in order to elucidate cancer onset mechanisms, so that the method can be effectively utilized in search of target molecules for a new medicament. | 06-20-2013 |
| 20130196364 | RAPID ANTIBIOTIC SUSCEPTIBILITY TESTING SYSTEM BASED ON BACTERIAL IMMOBILIZATION USING GELLING AGENT, ANTIBIOTIC DIFFUSION AND TRACKING OF SINGLE BACTERIAL CELLS - A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results. | 08-01-2013 |
| 20130196365 | ANTIBIOFILM NANOPOROUS NANOSTRUCTURES AND METHOD TO PRODUCE SAME - Durable nanoporous nanostructured materials that modify, eliminate and destroy biofilms that may develop due to the presence of bacteria, fungi and other microbes and method for making the same. Such nanoporous nanostructures may be deposited as coatings on a substrate and such coatings may include at least one nanopore and a plurality of nanoparticles which adhere to the substrate and/or other particles. The nanostructure can be produced using a single-sided electrode arrangement which is configured to produce an electrical arc or discharge at one end of an electrode and to emit the nanoparticles. The nanoparticles form a non-porous framework which delineates any nanopores and which can be deposited as one or more layers of nanothickness. Such nano structures may be resistant to removal from the substrate. Also described are testing methods and apparatus for the quick, accurate and simple evaluation of the efficacy of the antibiofilm properties of the nanoporous nano structure. | 08-01-2013 |
| 20130203106 | Apparatus and Method for Performing Experiments on Live Cells - An apparatus ( | 08-08-2013 |
| 20130210062 | In Vitro Cellular Bioassay for Neurotoxicity Testing - The present invention provides neurotoxicity and developmental neurotoxicity screening methods employing primary cultured neurons from | 08-15-2013 |
| 20130210063 | COMPOSITIONS AND ASSAYS FOR DETERMINING CELL VIABILITY - Provided are compositions and methods useful in evaluation of cell health and metabolism, cell viability, proliferation, and the effects of compounds on these qualities. The assays provided are rapid, robust, nontoxic and suitable for use with high throughput devices. | 08-15-2013 |
| 20130217063 | RAPID MICROBIAL DETECTION AND ANTIMICROBIAL SUSCEPTIBILITY TESTING - A method for the detection of microorganisms in a sample comprising contacting said sample with a biosensor concentration module, allowing microorganisms to grow for a first period of time and detecting growth of discrete microorganisms as an indication of the presence of said microorganisms. | 08-22-2013 |
| 20130280755 | THERAPEUTIC METHODS FOR SOLID DELIVERY - A fluid-delivery device includes an array of needles. The needle can deposit a hollow and/or porous tube into a tissue of a subject, and the porous tube can contain one or more fluid agents. The hollow and/or porous tube can control the rate at which the agents diffuse into the tissue. The device can simultaneously deliver a plurality of porous tubes along parallel axes in a tissue in vivo. If thereafter resected, the tissue can be sectioned for evaluation of an effect of each agent on the tissue; based on the evaluation, candidate agents or subjects can be selected or deselected for clinical trials or therapy. | 10-24-2013 |
| 20130288293 | Biological Markers for Tracking Distinctive Cellular Events and Uses Thereof - The invention relates to the field of medicine. More particularly, described herein are biological markers associated with phenotypical and/or genotypical alterations in a cell and to methods for assessing mammalian cells. Also described are methods for assessing the condition of a population of cells, methods for assessing carcinogenicity of compounds and methods for quantitatively determine the quality of cell populations. | 10-31-2013 |
| 20130295601 | Systems and Methods for Testing Drugs and Drug Delivery Systems - A system is provided that simulates the in vivo micro-environment of three-dimensional cellular structures or bodies, such as tumors. The system simulates the pressure gradients and fluid flows of the vascular and lymphatic systems as well as the interstitial and capillary transport mechanisms between the 3D cellular structure and the vascular and lymphatic systems. The system can be used to introduce drugs or drug delivery carriers to a tumor, for example, to assess the uptake capability and effect on the tumor. The system maintains the viability of the tumor cells for a sufficiently long period of time to permit testing of several different drugs and/or delivery carriers. | 11-07-2013 |
| 20130316392 | IN VITRO TUMOR IN DISH KIT AND METHOD - This invention is a method and kit for preparing a Tumor in Dish model for use in screening anti-cancer agents and analyzing cancer growth and development. | 11-28-2013 |
| 20130330765 | COMBINATIONS OF PHOSPHOINOSITIDE 3-KINASE INHIBITOR COMPOUNDS AND CHEMOTHERAPEUTIC AGENTS FOR THE TREATMENT OF HEMATOPOIETIC MALIGNANCIES - Combinations of PI3K inhibitor compounds having Formula I and chemotherapeutic agents, including stereoisomers, geometric isomers, tautomers, metabolites and pharmaceutically acceptable salts thereof, are useful for treating hematopoietic malignancies. Methods of using such combinations for in vitro, in situ, and in vivo diagnosis, prevention or treatment of such disorders in mammalian cells, or associated pathological conditions, are disclosed. | 12-12-2013 |
| 20130337491 | System and Method of Assessing a Wellbore Servicing Fluid or a Component Thereof - A wellbore servicing fluid assessment method comprising introducing test organisms into a first section of a first tub, allowing organisms less than a first size to pass into a second section of the first tub, allowing organisms less than the first size to flow into a first section of a second tub, allowing organisms less than a second size to pass into a second section of the second tub, allowing organisms less than the second size to flow into a first section of a third tub, allowing organisms less than a third size to pass into a second section of the third tub, selecting test organisms of a desired testing size, dividing the selected test organisms into a control group and test group, subjecting the test group to the wellbore servicing fluid or a component, and assessing the acceptability of the wellbore servicing fluid or component. | 12-19-2013 |
| 20140017721 | SPONTANEOUSLY IMMORTALIZED PROSTATE CANCER CELL LINE - This disclosure provides prostate cancer cell lines established from spontaneously immortalized, extremely tumorigenic and clonogenic primary prostate tumor. These cell lines represent unique cancer cell and cancer stem cell (CSC) models for preclinical prostate cancer studies and CSC-targeted drug development, which is of high value for pharmaceutic companies producing anti-cancer agents, as well as for the broad range of basic and translational research focused on cancer cell and CSC biology, stem cell behavior, cancer development and metastasis. | 01-16-2014 |
| 20140072999 | METHODS OF SIMULATING CHEMOTHERAPY FOR A PATIENT - The present invention provides methods for predicting or modeling a chemotherapy outcome for a given patient, to assist physicians in the selection of chemotherapeutic agents for individualized cancer treatment. | 03-13-2014 |
| 20140087416 | METHODS AND COMPOSITIONS FOR PRODUCING INDUCED HEPATOCYTES - The present invention relates to methods and compositions for use in generating induced hepatocytes by reprogramming non-hepatocyte cells. | 03-27-2014 |
| 20140093910 | D5 DESATURASE-DEFECTIVE MUTANT GENE AND USE THEREOF - It is an object of the present invention to provide a delta-5 desaturase-defective gene and uses of the gene and/or the mutant in algal transformation. | 04-03-2014 |
| 20140099662 | PHOTOTOXICITY TEST METHOD - The present invention provides a phototoxicity test method using human retinal pigment epithelial cells, and so on. | 04-10-2014 |
| 20140106395 | Hanging Droplet Plate - A hanging droplet plate ( | 04-17-2014 |
| 20140106396 | MASS SPECTROMETRIC MEASUREMENT OF MICROBIAL RESISTANCES - Microorganisms, particularly bacteria, are identified and characterized on the basis of a mass spectrometric measurement of their protein profiles with ionization by matrix-assisted laser desorption. In order to measure the microbial resistance to antibiotics, the protein profiles of microorganisms are measured after cultivation for a short time duration in nutrient media containing the antibiotics. | 04-17-2014 |
| 20140120573 | ENGINEERING INDIVIDUALLY ADDRESSABLE CELLULAR SPHEROIDS USING AQUEOUS TWO-PHASE SYSTEMS - Provided is multi-phase systems that may be used to prepare a three-dimension aggregate of cells referred to as a cellular spheroid. The multi-phase system includes a droplet of an aqueous polymer phase within an immersion aqueous polymer phase. The droplet of the droplet aqueous polymer phase contains a three-dimensional aggregate of cells (cellular spheroid). Types of cells that may be used in the multi-phases system include stem cells and cancer cells. The cellular spheroids with the multi-phase system may be used to monitor cell growth in three-dimensional systems, or screen drugs in a three-dimension aggregate of cells. | 05-01-2014 |
| 20140134667 | Using Induced Pluripotent Stem Cells for Screening Anti-Neoplastic Agents - The invention uses induced pluripotent stem cells (iPSCs) for screening anti-neoplastic agents by examining the capability of a single agent, compound or drug, or a combination of multiple agents, compounds or drugs, for inhibiting or suppressing one or more neoplastic activities or processes, including aerobic glycolysis and neoplastic anabolism and, thus, inhibiting rapid growth and excessive reproduction of neoplastic cells. Agents, compounds or drugs may also be screened for their potential in inhibiting an invasion and/or migration of neoplastic cells into healthy or normal tissues and/or cells and a metastasis of neoplastic cells into other sites of the body. Anti-neoplastic agents, compounds and/or drugs found through these methods may represent broad-spectrum anti-neoplastic agents, compounds and/or drugs that preferentially target and damage neoplastic tumor or cancer cells but exert limited or minimal harm to normal or healthy cells. | 05-15-2014 |
| 20140134668 | ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY OF SWITCHGRASS-DERIVED EXTRACTIVES - Switchgrass is an increasingly important biofuel crop, but knowledge of switchgrass fungal pathogens is not extensive. The purpose of this research was to identify the fungal pathogens that decrease crop yield of switchgrass grown in Tennessee and to investigate a potential sustainable disease management strategy from a value-added by-product of the switchgrass biofuel conversion process. The specific objectives were 1) to identify and characterize prevalent fungal pathogens of switchgrass in Tennessee, 2) assess switchgrass seed produced in the United States for seedborne fungal pathogens, and 3) evaluate switchgrass extractives for antimicrobial activity against plant pathogens. | 05-15-2014 |
| 20140134669 | POLYMYXIN DERIVATIVES AND USES THEREOF - The present invention relates to a polymyxin derivative wherein R1, R2 and R3 are optional and R1, R2, R3, R5, R8 and R9 are cationic or neutral amino acid residues selected so that the total number of positive charges at physiological pH is at least two but no more than three; and to a combination product comprising at least two such derivatives. The invention further relates to a method for treating, alleviating or ameliorating an infection in a subject, caused by a Gram-negative bacterium by administering a therapeutically effective amount of a derivative according to the present invention to said subject; to a method for sensitizing Gram-negative bacteria to an antibacterial agent by administering, simultaneously or sequentially in any order a therapeutically effective amount of said antibacterial agent and a derivative according to the present invention to said subject; to methods for developing novel antibiotics; for reducing the nephrotoxicity, for improving the pharmacokinetic properties of natural polymyxins and octapeptins; and for sensitizing clinically important bacteria to a host defence mechanism complement present in serum. Finally, the invention relates to a process for preparing such polymyxin derivatives. | 05-15-2014 |
| 20140154735 | TUMOUR CELL AND TISSUE CULTURE - The present invention relates to in vitro three-dimensional organotypic cell co-culture. Cultures of the invention comprise tumour cells three-dimensionally disposed within a matrix of matrix cells which are distinct from the tumour cells, wherein the co-culture does not comprise a basement membrane. The cultures are useful for the study of cancers, for testing anti-tumour agent efficacy, and for high-throughput screening of candidate drugs. | 06-05-2014 |
| 20140162308 | DEVICE AND METHOD FOR IDENTIFYING MICROBES AND COUNTING MICROBES AND DETERMINING ANTIMICROBIAL SENSITIVITY - A method of determining antimicrobial activity of an agent can include providing a well, wherein the well contains at least one antimicrobial agent, the well further including at least two electrodes. A sample of a microbe can be added into the well and a voltage pulsed between the electrodes. An electrical property can be sampled and recorded. In another aspect, a method of identifying at least one microbe includes taking a sample containing the at least one microbe, isolating the at least one microbe from the sample, dividing the at least one microbe into at least one well, wherein each well contains at least one antimicrobial agent and at least two electrodes. A voltage is pulsed between the at least two electrodes, an electrical property is sampled during the pulsing and recorded. In another aspect, a diagnostic device for detecting at least one microbe is presented. | 06-12-2014 |
| 20140178922 | SELECTIVE CULTURE MEDIUM AND METHOD FOR DETECTING CARBAPENEM-RESISTANT BACTERIA IN A TEST SAMPLE - The present invention relates to a culture medium comprising a carbapenem, a carbapenemase activator and a M-type penicillin. It also relates to a method for detecting carbapenem-resistant bacteria in a test sample using said culture medium. | 06-26-2014 |
| 20140212915 | METHOD FOR PREPARING HUMAN NEOPLASTICALLY TRANSFORMED CELLS - A method for preparing neoplastically transformed cells from human-derived cells, including the step of introducing human telomerase catalytic subunit (hTERT) gene, SV40 small T antigen (SV40ST) gene, and an antisense oligonucleotide derived from human 28S rRNA into the human-derived cells. The method for preparing neoplastically transformed cells from human-derived cells can be utilized when a variety of human normal cells are induced to be neoplastically transformed in order to elucidate cancer onset mechanisms, so that the method can be effectively utilized in search of target molecules for a new medicament. | 07-31-2014 |
| 20140248657 | METHOD FOR PRODUCING INSOLUBLE AGGREGATE OF NEURODEGENERATIVE-DISEASE-RELATED PROTEIN - The purpose of the present invention is to develop a method for producing a large amount of an insoluble aggregate that is equivalent to an insoluble aggregate formed in the brain of a patient. A method of producing an insoluble aggregate of a neurodegenerative-disease-related protein according to the present invention comprises the steps of: (1) introducing an insoluble fraction originated from the brain of a neurodegenerative disease patient into a cultured cell in which the neurodegenerative-disease-related protein can be expressed in a constitutive manner; (2) culturing the cultured cell into which the insoluble fraction has been introduced; and (3) extracting separating an insoluble fraction from the cultured cell. Optionally, the method may additionally comprise a step of amplifying the insoluble aggregate of the neurodegenerative-disease-related protein in the cultured cell. | 09-04-2014 |
| 20140255979 | METHOD OF TESTING A DISINFECTANT OR ANTIBIOTIC USING PHENANTHRIDIUM DERIVATIVES - The disclosure provides for a method of testing a disinfectant or antibiotic using phenanthridium derivatives with a 2 | 09-11-2014 |
| 20140273074 | YEAST SCREENS FOR TREATMENT OF HUMAN DISEASE - Screening methods for identifying substances that provide therapeutic value for various diseases associated with protein misfolding are provided. Genetic and chemical screening methods are provided using a yeast system. The methods of the invention provide a rapid and cost-effective method to screen for compounds that prevent protein misfolding and/or protein fibril formation and/or protein aggregation which includes numerous neurodegenerative diseases including Parkinson's disease, Alzheimer's disease, Huntington's disease as well as non-neuronal diseases such as type 2 diabetes. | 09-18-2014 |
| 20140287454 | SUSCEPTIBILITY TO SELECTIVE CDK9 INHIBITORS - The present invention relates to a method of selecting (a) cell(s), (a) tissue(s) or (a) cell culture(s) with susceptibility to a selective CDK9 inhibitor. Also a method for determining the responsiveness of a mammalian tumor cell or cancer cell to treatment with a selective CDK9 inhibitor is described herein. In particular, the present invention provides for an in vitro method for the identification of a responder for or a patient sensitive to a selective CDK9 inhibitor, whereby the patient is suspected to suffer from NUT midline carcinoma (NMC). The present invention also relates to a method of monitoring or predicting the efficacy of a treatment of NUT midline carcinoma (NMC), wherein treatment with a selective CDK9 inhibitor is in particular envisaged. Also the use of a (transgenic) non-human animal or a (transgenic) cell having at least one rearrangement in the NUT gene for screening and/or validation of a medicament for the treatment NUT midline carcinoma (NMC) is described. Furthermore, a kit useful for carrying out the methods described herein as well as an oligo- or polynucleotide capable of detecting rearrangements in the NUT gene are provided. | 09-25-2014 |
| 20140295483 | APPARATUS AND METHOD FOR IN-VITRO DRUG SCREENING FOR CANCER METASTASIS - The present invention includes an apparatus and methods for detecting cancer cell metastasis, the apparatus comprising: a multi-well plate for growing cells in tissue culture; one or more shafts, cones, or fins that fit within one or more of the wells of the multi-well plate and that can be positioned in close proximity to the bottom of the one or more wells; a rotational motor connected to drive the one or more shafts, cones, or fins to rotate, such that rotation of the shafts, cones, or fins creates fluid flow within the one or more wells; and a detector capable of measuring cells within the wells. | 10-02-2014 |
| 20140349333 | METHOD FOR INSPECTING SUSCEPTIBILITY OF BACTERIA OR FUNGI TO ANTIMICROBIAL DRUG AND SYSTEM FOR USE IN THE SAME - The present invention provides a novel method capable of easily and rapidly inspecting the susceptibility of bacteria or fungi to an antimicrobial drug and an inspection system for use in the method. The inspection method of the present invention is a method for inspecting susceptibility of bacteria or fungi to an antimicrobial drug using a micro-device having a flow channel, including: an incubating step of incubating a mixture of the antimicrobial drug and a bacterial suspension to be inspected in the flow channel of the micro-device; and a detecting step of detecting bacteria or fungi derived from the bacterial suspension to be inspected in an observation area of the flow channel of the micro-device. The detecting step can be performed by detecting an increase or decrease in the number of or a change in shape of bacteria or fungi derived from the bacterial suspension to be inspected in the observation area by a microscope or the like. | 11-27-2014 |
| 20150010940 | METHOD FOR THE RAPID DETERMINATION OF SUSCEPTIBILITY OR RESISTANCE OF BACTERIA TO ANTIBIOTICS - A method of rapidly evaluating the susceptibility of a strain of bacteria to a cell wall synthesis inhibiting antibiotic based on an assessment of cell enlargement in response to doses of the cell wall synthesis inhibiting antibiotic which are correlated to breakpoints of bacterial susceptibility. | 01-08-2015 |
| 20150017680 | TEST APPARATUS FOR TESTING THE MICROBIAL ACTIVITY ON SURFACES - The present invention relates to a device comprising a sample receiving appliance, a receiving appliance for test organisms, preferably for a suspension comprising test organisms, in particular for a bacterial suspension, and a jet pump appliance, wherein the jet pump appliance is, or can be brought, into active connection with the receiving appliance, and wherein the jet pump appliance is designed and installed in order, by means of a propellant medium having a higher pressure than atmospheric pressure at the location of installation of the device to spray test organisms in the form of an aerosol in the direction of the sample receiving installation, wherein the device has an installation for controlling a reproducible pressure of the propellant medium during the spraying of the test organisms and also the use of a device according to the invention. | 01-15-2015 |
| 20150056648 | METHODS OF DETERMINING BIOCIDE EFFICACY OR MECHANISM OF ACTION USING FLOW CYTOMETRY - Disclosed are methods of determining biocide efficacy using flow cytometry. The methods can be used to determine the synergy between at least two biocides. | 02-26-2015 |
| 20150072373 | Measurement of Cell Growth and Drug Susceptibility by Resonant Mass Measurement - System and Method for measuring the growth of a bacterial culture and its response to one or more antimicrobials using measurement of mass of individual microbes. Methods include periodic sampling, determining change in mass and concentration, and comparing growth rates of cultures in nutrient broth vs. mixtures containing various antibiotic mixtures. A number of antimicrobials can be compared in one measurement by multiplexing or using multiple sensors to measure in parallel. Growth and antibiotic efficacy can be assessed at low concentrations at the onset of growth, typically within 1 to 2 hours. | 03-12-2015 |
| 20150079626 | METHOD OF OBTAINING A CELL POPULATION CONTAINING CANCER STEM CELLS - The present invention relates to a method of obtaining a cell population containing cancer stem cells, which comprises culturing iPS cells in the presence of culture supernatant of cancer cells. | 03-19-2015 |
| 20150087011 | DEVICE AND METHOD FOR THE DETERMINATION AND MONITORING OF WATER TOXICITY - A device for analysis and monitoring of toxicity in waters is described. The device is specifically devised for determining toxicity in waters in several samples at the same time and in quick time with a high degree of accuracy and precision. The device has application in the field of controlling and monitoring the water resources and in the field of the ecotoxicological analyses | 03-26-2015 |
| 20150087012 | METHOD FOR QUANTITATIVELY EVALUATING ANTIMICROBIAL ACTIVITY OF ANTIMICROBIAL SAMPLE USING DISC DIFFUSION METHOD AND SYSTEM FOR MEASURING ANTIMICROBIAL ACTIVITY USING THE SAME - A method for quantitatively evaluating the antimicrobial activity of an antimicrobial sample using a disc diffusion method, and a measurement system using the method are provided. The method comprises: (a) measuring a maximum inhibition zone width (IZW | 03-26-2015 |
| 20150087013 | URA5 GENE AND METHODS FOR STABLE GENETIC INTEGRATION IN YEAST - A novel gene encoding | 03-26-2015 |
| 20150147774 | EXPRESSION CONSTRUCT FOR YEAST AND A METHOD OF USING THE CONSTRUCT - The present disclosure provides an isolated linear expression cassette. The disclosed cassette comprises a bidirectional promoter; a first gene and a second gene that the genes are respectively and operably linked to one end of the bidirectional promoter; a terminator located immediately next to end of each gene; a 5′-homogolgous region to genomic context of interest and a 3′-homogolgous region to genomic context of interest respectively flanking 5′ end and 3′ end of the cassette; and a dominant selection marker residing within the construct and being arranged in between the flanking 5′-homogolgous region to genomic context of interest and a 3′-homogolgous region to genomic context of interest. Preferably, the cassette is capable of being integrated into genome of transformed yeasts upon being transported into the transformed cell. | 05-28-2015 |
| 20150293077 | METHODS FOR DETERMINING THE EFFECT OF AN AGENT ON TISSUE STEM CELLS - Herein, we describe a direct in vitro method that identifies agents that are toxic against natural human tissue stem cells. We provide a novel schedule for culturing any cell population containing homologous tissue stem cells that allows the number and cell kinetics of tissue stem cells, transient cells, and terminally differentiated cells within the population to be monitored. Using the passage schedule together with determination of a growth curve for the population, one can determine whether or not an agent is toxic to tissue stem cells, or to transient cells and/or terminal cells. The same method can also be used to identify agents that act positively on tissue stem cells and the other specific cell types. | 10-15-2015 |
| 20150299256 | ANTIBIOTIC PEPTIDES - The invention relates to a peptide or peptide derivative having the general formula: Sub | 10-22-2015 |
| 20150299663 | METHOD FOR CULTURING A SUBPOPULATION OF CIRCULATING EPITHELIAL TUMOUR CELLS FROM A BODY FLUID - The invention relates to a method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid of a human or animal suffering from an epithelial tumour, wherein cells contained in the body fluid each containing at least one cell nucleus are separated from the body fluid and cultured over at least 24 hours in suspension, with formation of spheroids. | 10-22-2015 |
| 20150315625 | RAPID ANTIBIOTIC SUSCEPTIBILITY TESTING SYSTEM BASED ON BACTERIAL IMMOBILIZATION USING GELLING AGENT, ANTIBIOTIC DIFFUSION AND TRACKING OF SINGLE BACTERIAL CELLS - A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results. | 11-05-2015 |
| 20150337251 | MICROFLUIDIC CHIP FOR CULTURING MICROORGANISMS AND METHOD OF OPERATING THE SAME - The present invention provides a microfluidic chip for culturing microorganism and a method of operating the same. The microfluidic chip includes a first input unit, a second input unit, a connection unit connected to the first and second input units, a plurality of control valves, and a ring-shaped storage structure connected to the connection unit and having first and second growth chambers that store a microbial solution. The first and second input units provide first and second solutions, respectively. The first solution is transferred into the first or second growth chamber through the connection unit to treat and discharge a portion of the microbial solution. The second solution is transferred into the first or second growth chamber to discharge the first solution. The control valves are actuated to mix the second solution and remainder of the microbial solution in the ring-shaped storage structure. | 11-26-2015 |
| 20150337353 | RAPID ANTIBIOTIC SUSCEPTIBILITY TESTING SYSTEM BASED ON BACTERIAL IMMOBILIZATION USING GELLING AGENT, ANTIBIOTIC DIFFUSION AND TRACKING OF SINGLE BACTERIAL CELLS - A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results. | 11-26-2015 |
| 20150361148 | AMYLOID BETA EXPRESSION CONSTRUCTS AND USES THEREFOR - Disclosed are yeast expression constructs encoding a polypeptide containing a signal sequence, a Golgi-directing pro sequence, and a human amyloid beta protein, and mammalian expression constructs encoding a polypeptide containing a selected signal sequence and a human amyloid beta protein. Also disclosed are methods of screening cells to identify compounds that prevent or suppress amyloid beta-induced toxicity and genetic suppressors or enhancers of amyloid beta-induced toxicity. Compounds identified by such screens can be used to treat or prevent neurodegenerative disorders such as Alzheimer's disease. | 12-17-2015 |
| 20150361392 | LIQUID CULTURING OF EPITHELIAL STEM CELLS - Provided herein is a method of culturing epithelial stem cells and tissue fragments comprising epithelial stem cells in liquid cultures. | 12-17-2015 |
| 20150376676 | METHOD TO GENERATE NOVEL BIOACTIVE MOLECULES - The present invention describes a method to generate new chemical entities (NCEs) that have well-defined activities such as, but not limited to, anti-bacterial, antifungal and anthelmintic effects. The NCEs are generated through adaptive evolution of one microbe (the producer) against another organism or cell type (the target). The producer is made to compete against the target over time by co-culturing the two together and serially passing the producer organism until the producer adaptively evolves by synthesizing an NCE(s) that inhibits growth of or kills the target. The molecular structure of the chemical entity (or entities) is then elucidated using tools from genomics, molecular biology, computational biology, analytical chemistry, organic chemistry and related fields. | 12-31-2015 |
| 20160010137 | Anti-Biofilm Screening Assays | 01-14-2016 |
| 20160022638 | METHODS OF TREATMENT ASSOCIATED WITH THE GRANULOCYTE COLONY-STIMULATING FACTOR RECEPTOR - Some embodiments include methods for treating, preventing, reversing, halting, or slowing the progression of cancer, comprising administering to a subject in need thereof an effective amount of one or more chemotherapeutic agents, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition, wherein at least one of the chemotherapeutic agents is a cytotoxic granulocyte colony-stimulating factor receptor (GCFR) modulator. Methods are also disclosed for treating, preventing, reversing, halting, or slowing the progression of a hematopoietic disorder, comprising administered a therapeutically effective cytotoxic amount of a GCFR modulator to a subject in need thereof. | 01-28-2016 |
| 20160054303 | COMPOSITIONS AND ASSAYS FOR DETERMINING CELL VIABILITY - Provided are compositions and methods useful in evaluation of cell health and metabolism, cell viability, proliferation, and the effects of compounds on these qualities. The assays provided are rapid, robust, nontoxic and suitable for use with high throughput devices. | 02-25-2016 |
| 20160075985 | MICROFLUIDIC MULTI-WELL-BASED CELL CULTURE TESTING DEVICE - A microfluidic multi-well-based cell culture testing device is provided. The multi-well-based cell culture testing device has an array structure of a plurality of aligned microfluidic well units. Each of the microfluidic well units comprises an inlet through which a first fluid enters, an accommodation compartment adapted to accommodate a second fluid therein, a microfluidic channel through which the first fluid flows, and an air outlet adapted to facilitate the entering of the first fluid. | 03-17-2016 |
| 20160076071 | METHOD FOR THE DETERMINATION OF THE PRESENCE OF AN ANTIBIOTIC IN A FLUID - The present invention provides a method and test for the determination of the presence or absence of an antibiotic in a sample such as milk. | 03-17-2016 |
| 20160091485 | MARKERS FOR EZH2 INHIBITORS - The invention provides methods of detecting an EZH2 mutation and associated epigenetic markers in a cancer cell, methods cancer diagnosis and methods of screening for EZH2 inhibitors. | 03-31-2016 |
| 20160091487 | ENGINEERING INDIVIDUALLY ADDRESSABLE CELLULAR SPHEROIDS USING AQUEOUS TWO-PHASE SYSTEMS - Provided are multi-phase systems that may be used to prepare a three-dimension aggregate of cells referred to as a cellular spheroid. The multi-phase system includes a droplet of an aqueous polymer phase within an immersion aqueous polymer phase. The droplet of the droplet aqueous polymer phase contains a three-dimensional aggregate of cells (cellular spheroid). Types of cells that may be used in the multi-phases system include stem cells and cancer cells. The cellular spheroids with the multi-phase system may be used to monitor cell growth in three-dimensional systems, or screen drugs in a three-dimension aggregate of cells. | 03-31-2016 |
| 20160097027 | Engineering Of A Novel Breast Tumor Microenvironment On A Microfluidic Chip - A microfluidic device for more accurately modeling the in vitro environment in which cancer occurs is disclosed. The device comprises a surface defining one or more microfluidic channels that may further comprise one or more endothelial cells, a first three dimensional scaffold comprising one or more cancer cells that is spatially separated from the one or more microfluidic channels, and a second three dimensional scaffold comprising one or more stromal cells, at least a portion of which is interposed between the one or more microfluidic channels and the first three dimensional scaffold. The second three dimensional scaffold is in fluid communication with both the first three dimensional scaffold and the one or more microfluidic channels. The device can be used to assay anti-cancer agents, or as a system for modeling the growth, behavior, or metastasis and tumor formation of cancer cells. | 04-07-2016 |
| 20160102334 | RAPID ANTIMICROBIAL SUSCEPTIBILITY TEST, BASED ON AN ANALYSIS OF CHANGES IN MORPHOLOGY AND GROWTH PATTERN OF A MICROBIAL CELL UNDER DIFFERENT CONCENTRATIONS OF VARIOUS ANTIMICROBIAL AGENTS, AND AUTOMATED CELL IMAGE ANALYSIS SYSTEM THEREFOR - Provided are a rapid antimicrobial susceptibility test, based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and an automated cell image analysis system therefor. The antimicrobial susceptibility test is rapidly performed based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and this makes it possible to obtain highly reliable test results faster by six to seven times than the standard method recommended by Clinical and Laboratory Standards Institute (CLSI). | 04-14-2016 |
| 20160103122 | METHOD FOR THE CREATION OF A DATABASE FOR INITIAL ASSESSMENT OF THE EFFECTIVENESS OF ACTIVE AGENTS IN TUMOR THERAPY - The method comprises the steps wherein the tumour cells from a tumor tissue sample previously taken from a particular patient are incubated ex vivo with the active agent concerned and the IC50 value thereof for these cells is determined; wherein the same patient is treated with this active agent in accordance with evidence-based therapy rules; wherein the therapy outcome is documented and classified; and wherein the documentation and/or classification of the therapy outcome obtained is assigned to the IC50 value determined ex vivo. | 04-14-2016 |
| 20160108407 | IMPROVED SURFACE DISPLAY OF FUNCTIONAL PROTEINS IN A BROAD RANGE OF GRAM NEGATIVE BACTERIA - The present invention relates to a method for the surface display of a recombinant polypeptide on the surface of a host cell, said method comprising the steps: (a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide, (ii) a portion encoding the recombinant polypeptide to be displayed, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the trans porter domain of an EhaA protein, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell. | 04-21-2016 |
| 20160109432 | METHODS AND MATERIALS FOR ASSESSING PLURIPOTENCY OF STEM CELL POPULATIONS - This document provides methods and materials for assessing the pluripotency of stem cell populations. For example, methods and materials for using a DNA damaging agent (e.g., etoposide) to assess the pluripotency of a population of stem cells (e.g., a population of human induced pluripotent stem cells) are provided. | 04-21-2016 |
| 20160114325 | FLUORINATED PICKERING EMULSION - Described here is a composition comprising amphiphilic silica nanoparticles, wherein the silica nanoparticles are partially fluorinated. Also described here is a method for droplet-based assay, comprising dispersing at least one aqueous droplet in a continuous fluorous phase in a microfluidic channel, wherein at least one amphiphilic silica nanoparticle is absorbed to the interface of the continuous fluorous phase and the aqueous droplet, and wherein the silica nanoparticle is partially fluorinated. Further described here is a method for droplet-based assay, comprising dispersing at least one aqueous phase droplet in a continuous fluorous phase in a microfluidic channel, wherein the continuous fluorous phase comprises at least one partially fluorinated amphiphilic particle adsorbed to an interface of the continuous fluorous phase and the aqueous phase droplet, and wherein the aqueous phase droplet comprises at least one hydrophilic polymer adsorbed to the amphiphilic particle at the interface. | 04-28-2016 |
| 20160168616 | PRODUCTION OF NONTOXIC RAW MATERIALS AND FINISHED PRODUCTS TESTED BY MEANS OF AN INNOVATIVE PROBIOTIC BACTERIA BASED METHOD FOR DETERMINING TOXICITY TOWARDS PROBIOTIC BACTERIA | 06-16-2016 |
| 20160178611 | THREE-DIMENSIONAL TRANSGLUTAMINASE-CROSSLINKED HYDROGEL FOR TUMOR ENGINEERING | 06-23-2016 |
| 20160376629 | Use of Bacterial Beta-Lactamase for In Vitro Diagnostics and In Vivo Imaging, Diagnostics and Therapeutics - Provided herein provided is an assay system for monitoring drug susceptibility of a pathogenic bacteria comprising color-producing substrates for a beta-lactamase of the pathogenic bacteria, an assay device for visibly detecting a product of the beta-lactamase on the substrate thereof and a reader configured to quantify the visibly detected product. Also provided is an in vitro method to determine susceptibility to a drug effective against a pathogenic bacteria, for example, a pathogenic Mycobacteria, that has a beta-lactamase activity. An excitation wavelength is delivered to a biological sample obtained from a subject having an infection from the pathogenic bacteria in the presence of a beta-lactamase substrate. The intensity of a signal, such as a fluorescent, luminescent or colorimetric signal, at an emission wavelength of a product of the beta-lactamase on the subject is correlated to drug susceptibility. | 12-29-2016 |
| 20170233786 | NOVEL BIOACTIVITY TESTING STRUCTURE FOR SINGLE CELL TRACKING USING GELLING AGENTS | 08-17-2017 |
| 20190144913 | MICROFLUIDIC TESTING SYSTEM WITH CELL CAPTURE/ANALYSIS REGIONS FOR PROCESSING IN A PARALLEL AND SERIAL MANNER | 05-16-2019 |
| 20190144914 | MODULAR PARALLEL/SERIAL DUAL MICROFLUIDIC CHIP | 05-16-2019 |
| 20220137029 | COMPOSITIONS AND METHODS FOR DISEASE DIAGNOSIS USING SINGLE CELL ANALYSIS - Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells. | 05-05-2022 |
