Class / Patent application number | Description | Number of patent applications / Date published |
435021000 | Involving phosphatase | 49 |
20080213814 | ENZYMATIC ASSAY FOR LSPR - The present invention provides a method of detecting changes in the refractive index at the surface of a localized surface plasmon resonance (LSPR) detection system. The method includes generating an insoluble product from an enzymatic substrate using an immobilized enzyme, wherein the insoluble product accumulates at a LSPR supporting surface. The method also includes detecting changes in the reflected or transmitted light of the surface arising from the presence of the insoluble product using LSPR. | 09-04-2008 |
20080254492 | IMMUNOASSAY METHOD, REAGENT KIT FOR DETECTING ALKALINE PHOSPHATASE, AND REAGENT KIT FOR IMMUNOASSAY - An immunoassay method comprising:
| 10-16-2008 |
20090081717 | Compositions and Methods For Signal Generation - Improved assay systems comprise a substrate that is modified to include a solubilizing group and/or an at least binary solvent system to provide significantly enhanced signal strength and signal-to-noise ratio. In especially preferred aspects, the assay system is a chromogenic, electrochemical, and/or luminogenic assay system in which an indoxyl-type substrate has a solubilizing group covalently attached to the substrate and/or in which a polar and non-protic solvent is used as a co-solvent. | 03-26-2009 |
20090093009 | MASS SPECTROMETRY METHOD FOR MEASURING THIAMINE IN BODY FLUID - Provided are methods for determining the amount of total thiamine in a body fluid sample using liquid chromatography and mass spectrometry. Total thiamine is converted to free thiamine by treatment with an acid phosphatase prior to thiamine separation and quantification. | 04-09-2009 |
20090098589 | Method - There is provided a method for the detection of phytase activity in a sample, which comprises bringing into association a phytase substrate and said sample, and measuring the level of an organic metabolite of said phytase substrate. | 04-16-2009 |
20090148877 | ENZYMATIC METHODS FOR MEASURING PLASMA AND TISSUE SPHINGOMYLELIN AND PHOSPHATIDYLCHOLINE - A method for measuring sphingomyelin and phosphatidylcholine comprising incubating sphingomyelin and phosphatidylcholine with bacterial sphingomyelinase and bacterial phospholipase D, alkaline phosphatase, choline oxidase, peroxidase, N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline, and 4-aminoantipyrine, preferably for about 45 minutes. A blue dye is generated. | 06-11-2009 |
20090170147 | Forensic test kit and method for the detection of semen - The present invention relates to a forensic test kit and method for the detection of semen. This kit is comprised of two main components: acid phosphatase (AP) test strips and prostate specific antigen (PSA) test strips, which work together to provide evidence of semen on fabrics and other surfaces. The AP strips are used first to test an item, which provides presumptive evidence of semen, while follow-on testing by the more sensitive and specific PSA strips provides confirmatory evidence. | 07-02-2009 |
20090275067 | METHOD OF ACQUIRING HEPATIC OVAL CELLS - Provided are a method of isolating and identifying hepatic oval cells from the liver, and a method of single cell culture of hepatic oval cells. A method of separating and/or acquiring hepatic oval cells from a mammal, comprising a step for examining the expression of CD133, CD45 and Ter119. Particularly, hepatic oval cells that exhibit the pattern of CD133 | 11-05-2009 |
20090325207 | NOVEL PHOSPHODIESTERASE AND GENE THEREOF - The present invention is to provide a novel phosphodiesterase and a gene thereof, specifically, Type 11 phosphodiesterase (PDE11) and a gene thereof, more specifically, a phosphodiesterase selected from (A) a protein having an amino acid sequenced shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: 39, and (B) a protein having an amino acid sequence shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: in which one or several amino acids are deleted, substituted or added, and having an activity of hydrolyzing a cyclic nucleotide, and a gene thereof, and a method of characterizing, identifying and selecting a phosphodiesterase inhibitor by using the same. | 12-31-2009 |
20100028925 | METHOD AND DEVICE FOR THE ANALYSIS OF ENZYME ACTIVITY IN FLUIDS - An assay in which alkaline phosphatase is detected on one of a number of membranes held on a holder. A liquid sample and a substrate are combined on the membrane. If the enzyme is present, the substrate is cleaved. The products of this cleavage are optically detectable. | 02-04-2010 |
20100086957 | METHOD FOR MONITORING INTRACELLULAR TYROSINE PHOSPHATASE ACTIVITY - The invention relates in general to tyrosine dephosphorylation. More specifically, the invention relates to methods and compositions for monitoring intracellular tyrosine dephosphorylation at the single cell level. The invention further relates to techniques that can be used as aid in the development of novel therapeutics, and monitor regulation of intracellular tyrosine phosphatase activity at the single cell level. | 04-08-2010 |
20100255521 | Fluorescent substrates for Detecting organophosphatase enzyme activity - Disclosed are compounds of the formula (I): wherein R | 10-07-2010 |
20100261212 | KINETICS OF MOLECULAR RECOGNITION MEDIATED NANOPARTICLE SELF-ASSEMBLY - A method of multiple protein biomarker detection, comprising providing at quantum dot-antibody conjugates that have an affinity for at least two different protein biomarkers; contacting the conjugates with a sample from a subject; allowing the proteins to bridge the antibodies, forming protein biomarker/quantum dot-antibody conjugate agglomerates; detecting the presence of the biomarkers by excitation of the agglomerates. | 10-14-2010 |
20100261213 | METHOD FOR LARGE SCALE PREPARATION OF THE ACTIVE DOMAIN OF HUMAN PROTEIN TYROSINE PHOSPHATASE WITHOUT FUSION PROTEIN - The present invention relates to protein tyrosine phosphatase (PTP) and a method for preparing the same, precisely, a method for expressing PTP active domain with high activity and stability without help of a fusion protein, by using computer based protein structure prediction technique. PTP prepared by the method of the present invention can be effectively used as a protein for high efficiency drug screening for the development of a novel drug, as an antigen protein for the construction of a selective antibody and as a protein for the studies of PTP structure and functions. | 10-14-2010 |
20100273197 | SMART ENZYME-CONTAINING CATALYTIC PEELABLE COATINGS - A coating material that is applied to a surface for absorbing a toxic chemical and that is removable from the surface by the use of mechanical force is disclosed. The coating material comprises one or more polymer coatings, one or more purified enzymes immobilized or entrapped in the polymer coating for protecting a surface from contamination by one or more toxic chemicals, one or more reporter indicators that are responsive to changes in pH in the presence of the toxic chemical for detecting and disclosing the location of said toxic chemical, and one or more buffers for establishing and/or maintaining the pH of the coating ranging from 5 to 10. Preferably, the reporter indicator is one or more pH-sensitive dyes or dye blends that change colors in the presence of the toxic chemical. A method for absorbing, detoxifying, detecting and disclosing of a toxic chemical is provided. | 10-28-2010 |
20100291609 | Reducing Optical Interference in a Fluidic Device - This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications. In particular, the medical device reduces interference with an optical signal which is indicative of the presence of an analyte in a bodily sample. | 11-18-2010 |
20100311095 | ASSESSMENT OF PHYSIOLOGICAL CONDITIONS - The present invention relates to a method for assessing in vitro the balance and the overall dynamics of a physiological condition, wherein a) a first biochemical marker representing a first biochemical process associated with the physiological condition is measured, b) a second biochemical marker representing a second biochemical process associated with the physiological condition is measured, c) the ratio of the results obtained in (a) and (b) is calculated, d) the square root of the sum of the squared results obtained in (a) and (b) is calculated and, e) wherein the ratio calculated in (c) is used to assess the balance of the physiological condition and wherein the square root calculated in (d) is used to assess the overall dynamics of the physiological condition. | 12-09-2010 |
20110039288 | REACTION MEDIUM FOR METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) BACTERIA - A reaction medium for detecting and/or identifying methicillin-resistant | 02-17-2011 |
20110065138 | Quantitative Method For Detecting Yessotoxins In Fishery Products On Based On The Activation That The Toxin Produces In Cellular Phosphodiesterases And Therapeutic Use Of This Activation - The present invention relates to a quantitative method for detecting yessotoxins in fishery products based on the activation the toxin produces on cellular phosphodiesterases and the therapeutic use of this activation. The cellular target of yessotoxin (YTX) and its analogs is the activation of phosphodiesterases (PDEs). The PDEs-YTX bond produces a measurable signal. The bond can be quantified by means of an affinity biosensor or by fluorescence. The biosensor detects biomolecular interactions and allows determining the presence of YTX due to its interaction with PDEs. The variations in the degradation rate of the fluorescent derivative anthraniloyl-cAMP are determined by means of plate fluorescence. The rate at which the PDEs degrade this molecule increases in the presence of YTX. YTX inhibits immunological activation of mastocytes in rats and induces a cytotoxic effect in human hepatocarcinoma cells, which implies two therapeutic uses of YTXs as an antiallergic and antitumor compound. | 03-17-2011 |
20110065139 | DIAGNOSTIC DEVICE FOR IDENTIFYING RUPTURE OF MEMBRANE DURING PREGNANCY - A diagnostic device is provided for distinguishing between amniotic fluid and urine in female secretion. The device can be employed as a panty shield or can be adhered onto a panty shield or can be employed in a simple pad that is pressed against a substrate provided with female secretion. | 03-17-2011 |
20110076708 | Cartilage intermediate layer protein 2 C1 and its use to differentiate osteoarthritis from rheumatoid arthritis and non-disease conditions - A method for differentiation of osteoarthritis from rheumatoid arthritis and non-disease conditions in a sample, comprising measuring in the sample the concentration of human cartilage intermediate layer protein 2 (CILP-2) in body fluids and more specifically, measuring in the sample the concentration of the N-terminal part of CILP-2 (2C1) or fragments thereof. | 03-31-2011 |
20110117587 | SINGLE MOLECULE DETECTION PLATFORM, MANUFACTURING METHOD THEREOF AND METHOD USING THE SAME - A single molecule detection platform is disclosed. The single molecule detection platform comprises a light-transmissive substrate, a plurality of spherical particles and a thin film. The surface of the light-transmissive substrate is etched to form a plurality of cone-shaped structures. Each spherical particle is disposed on top of each cone-shaped structure. The sizes of the plurality of spherical particles are suitable to allow only a single protein to be attached to each spherical particle. The thin film is deposited on the surface of the plurality of cone-shaped structures and acts as a reflective layer of one-dimensional waveguide. The plurality of spherical particles is not covered by the thin film. | 05-19-2011 |
20110229920 | SCREENING METHODS FOR IDENTIFYING SPECIFIC STAPHYLOCOCCUS AUREUS INHIBITORS - Methods of inhibiting | 09-22-2011 |
20120164673 | PTP1B INHIBITORS - Described herein are agents, including antibodies, antibody fragments, polypeptides and organic small molecules, that bind reversibly inactive PTP1B (designated herein in the alternative as PTP1B-SN or PTP1B-OX) and stabilize it (stabilize PTP1B-OX) in such a manner that they inhibit reactivation of reversibly oxidized, inactive PTP1B by reduction (by reducing agent) and have no substantial direct inhibitory effect on phosphatase activity/PTP1B activity (for example, as detectable in assays in vitro). | 06-28-2012 |
20120208223 | FLUORESCENCE RESONANCE ENERGY TRANSFER ENZYME SUBSTRATES - Disclosed are compounds of formula (I) wherein D | 08-16-2012 |
20130004981 | SYSTEM AND METHOD FOR SELECTING MOLECULE INTERACTING WITH PROTEIN PHOSPHATASE - A system and a method for selecting a molecule interacting with protein phosphatase are disclosed. The fluorescence protein detecting system for selecting a molecule interacting with protein phosphatase includes: a scaffold subunit A; a regulatory subunit B; a catalytic subunit C; a first fluorescence protein, which includes a first part and a second part, wherein the first part and the second part are separated from each other; and a second fluorescence protein, wherein the emission spectrum of the second fluorescence protein overlaps with the excitation spectrum of the first fluorescence protein. When the second part of the first fluorescence protein is fused with the regulatory subunit B, the second fluorescence protein is fused with the catalytic subunit C, alternatively. When the second part of the first fluorescence protein is fused with the catalytic subunit C, the second fluorescence protein is fused with the regulatory subunit B. | 01-03-2013 |
20130023003 | KIT INCLUDING SUBSTRATES WITH VARIOUS SURFACE CHEMISTRIES - In one embodiment of the invention, there is provided a kit including: (i) a plasma polymerized surface having first and second regions, said first region including a first concentration of carboxylic acid groups on said plasma polymerized surface and said second region including a second concentration of carboxylic acid groups on said plasma polymerized surface, wherein said first concentration and said second concentration are different; (ii) a first plasma polymerized secondary substrate having carboxylic acid groups disposed consistently thereacross at a concentration equal to said first concentration; and, (iii) a second plasma polymerized secondary substrate having carboxylic acid groups disposed consistently thereacross at a concentration equal to said second concentration. | 01-24-2013 |
20130029366 | PTP1B INHIBITORS - Described herein are agents, including antibodies, antibody fragments, polypeptides and organic small molecules, that bind reversibly inactive PTP1B (designated herein in the alternative as PTP1B-SN or PTP1B-OX) and stabilize it (stabilize PTP1B-OX) in such a manner that they inhibit reactivation of reversibly oxidized, inactive PTP1B by reduction (by reducing agent) and have no substantial direct inhibitory effect on phosphatase activity/PTP1B activity (for example, as detectable in assays in vitro). | 01-31-2013 |
20130040329 | ANALYTICAL APPLICATIONS OF ENZYMATIC GROWTH OF FLUORESCENT QUANTUM DOTS - Methods for the detection/quantification of the enzymatic activity of hydrolases in a sample, wherein the hydrolase catalyses a hydrolysis reaction of a substrate which yields either H | 02-14-2013 |
20130095512 | DEVICE FOR SORTING, CLASSIFYING, AND ASSAYING PARTITION BEHAVIOR OF CELL MEMBRANE BIOMOLECULES AND METHODS BASED THEREON - A biomolecule partitioning device (BPD) is provided that can be used to separate and sort membrane species into raft-like membrane regions without using detergent or crosslinkers. The BPD can comprise one or more microfluidic channels coated with coexistent lipid phases (raft-like and fluid-like lipid compositions) as a contiguous supported lipid bilayer (SLB). The geometry of the phases can be patterned with spatial and temporal control within each channel. Methods for the separation and sorting are also provided. The method can comprise the steps of introducing cell membrane species into an SLB; patterning coexistent phases; applying an electric field or hydrodynamic flow to move the species; sorting migrating species into regions based on their partitioning preference; and collecting sorted species in a quantification area. The BPD can also be used to measure partitioning kinetics or to assay for activity changes of biomolecules as a function of local lipid environment. | 04-18-2013 |
20130115644 | MASS SPECTROMETRY METHOD FOR MEASURING THIAMINE IN BODY FLUID - Provided are methods for determining the amount of total thiamine in a body fluid sample using liquid chromatography and mass spectrometry. Total thiamine is converted to free thiamine by treatment with an acid phosphatase prior to thiamine separation and quantification. | 05-09-2013 |
20130224779 | METHOD FOR LARGE SCALE PREPARATION OF THE ACTIVE DOMAIN OF HUMAN PROTEIN TYROSINE PHOSPHATASE WITHOUT FUSION PROTEIN - The present invention relates to methods for identifying inhibitors or activators of protein tyrosine phosphatase (PTP). In some examples, the methods utilize a PTP active domain with high activity and stability expressed without help of a fusion protein, by using computer based protein structure prediction technique. PTP prepared by the disclosed method may also be used as an antigen protein for the construction of a selective antibody and as a protein for the studies of PTP structure and functions. | 08-29-2013 |
20130266977 | METHODS AND SYSTEMS FOR DETECTION OF MICROBES - Provided herein are methods, test units, systems, assays and kits for qualitative and/or quantitative detection of microorganisms or microbes. Some aspects of the invention utilize a test strip comprising a test area and a self-calibrating control area that permits quantitative measurements to be obtained rapidly at the test site. The test strip is ready-to-use and does not require any preparation. The present systems and methods provide valuable tools for sensitive early detection of microbial contamination. | 10-10-2013 |
20130323770 | SUBSTRATES AND METHODS FOR STAINING LIVE STEM CELLS - The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming. | 12-05-2013 |
20140004548 | SAMPLE COLLECTION AND BIOLUMINESCENT ANALYSIS SYSTEM | 01-02-2014 |
20140093897 | AROMATIC-CATIONIC PEPTIDES AND USES OF SAME - The present disclosure provides aromatic-cationic peptide compositions and methods of using the same. The methods comprise use of the peptides in electron transport and electrical conductance. | 04-03-2014 |
20140093898 | REDUCING INSOLUBLE DEPOSIT FORMATION IN ETHANOL PRODUCTION - The present inventors have surprisingly discovered that phytic acid tenaciously precipitates with soluble metals in food or fuel ethanol-processing fluid, producing insoluble organometallic salt deposit or scale on the processing equipment that must be removed in order to facilitate further ethanol processing. The present invention relates to converting phytic acid salts or phytates to inorganic phosphates to improve metal solubility and reduce deposition within processing equipment. | 04-03-2014 |
20140147875 | DISEASE SEVERITY INDEX FOR ASSESSMENT OF CHRONIC LIVER DISEASE AND METHOD FOR DIAGNOSIS OF THREE DISTINCT SUBTYPES OF PRIMARY SCLEROSING CHOLANGITIS - A Disease Severity Index (DSI) is provided for assessment of chronic liver disease in a patient using non-invasive liver function test results. A DSI was derived from non-invasive liver function test results based on hepatic blood flow. The DSI is used in methods for prediction of clinical outcomes, prediction of response to antiviral treatment, and assessment of progression of chronic liver diseases. Non-invasive methods to diagnose three distinct categories of patients with Primary Sclerosing Cholangitis (PSC) are provided. The methods can be used to diagnose PSC patients as Slow Progressors, Moderate Progressors and Rapid Progressors. | 05-29-2014 |
20140162301 | Method for Classifying the Presence or Absence of a Microorganism in a Biological Medium - The present invention relates to a method for determining the presence or absence of a microorganism, said method including the steps of: 1) providing an enclosure containing a liquid or semi-solid phase consisting of a biological medium capable of containing a living form of said microorganism, nutritional elements, and an enzymatic substrate which is specific to said microorganism and which can be metabolised into at least one VOC metabolite, and a gaseous phase adjacent to said liquid or semi-solid phase; 2) exposing at least said liquid or semi-solid phase to conditions that are propitious for said microorganism to metabolise said enzymatic substrate into a molecule of said VOC metabolite; and 3) determining, by optical transduction, the presence or absence of said VOC metabolite, characterised in that the latter interacts with a nanoporous matrix, said matrix being implemented in a form that is separate from said enzymatic substrate, and in that the detection, by optical transduction, of a change in the optical properties of said matrix indicates an interaction of said matrix with said metabolite. | 06-12-2014 |
20140162302 | PON1 AS A MARKER FOR HEART FAILURE - Provided herein are methods for assessing the risk a test subject with heart failure has of experiencing a major adverse cardiac event, requiring revascularization, requiring a heart transplant, requiring unscheduled hospitalization for heart failure, progression of heart failure status, or any combination thereof. Also provided herein are methods for assessing the risk a test subject has of developing heart failure. The present methods comprise determining the levels of paraoxonase 1 activity in the serum, non-chelated plasma, or both in the test subject and comparing the level of PON1 activity in the test subject's sample with a control or baseline value based on levels of PON1 activity in serum, non-chelated plasma, or both samples from a population of control subjects. Also provided herein are kits useful in assessing such risks. | 06-12-2014 |
20140220610 | METHOD AND CULTURE DEVICE FOR DETECTING YEASTS AND MOLDS - A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided. | 08-07-2014 |
20150050681 | METHOD FOR MEASURING CALCINEURIN ACTIVITY - The present invention relates to a method for measuring the activity of calcineurin in a biological sample wherein a kinase inhibitor is present in the assay reaction mix. | 02-19-2015 |
20150132784 | METHODS AND KITS FOR DETECTING A XEROPHILIC MICROORGANISM OR AN OSMOPHILIC MICROORGANISM - A method for detecting a xerophilic microorganism is provided. The method comprises providing a sample to be tested, an aqueous diluent comprising about 1.0 to about 10.2 weight percent glycerol, and a thin film culture device comprising a cold water-reconstitutable medium to facilitate the growth of a microorganism. The method further comprises mixing the sample with the aqueous diluent to form an inoculum, contacting a predefined amount of the inoculum with the reconstitutable medium to form an inoculated thin film culture device, incubating the inoculated thin film culture device for a period of time, and detecting a presence or an absence of colony of a microorganism. Kits for detecting a xerophilic microorganism according to the method are also provided. | 05-14-2015 |
20150299763 | SPLA2 CARDIOVASCULAR PROGNOSTIC AND DIAGNOSTIC MARKER - A method for determining an increased risk of mortality or of a cardiac and/or vascular event in a patient, comprising: | 10-22-2015 |
20150329897 | MICRO-ORGANISM DETECTION MEDIUM COMPRISING AT LEAST ONE ALKYL(THIO)GLYCOSIDE - A microorganism detection medium, said detection being based on showing the presence of a microbial enzyme activity chosen from esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase activities of microorganisms, preferably said microbial enzyme activity being an esterase activity, said medium comprising:
| 11-19-2015 |
20160011206 | SALIVARY BIOASSAY FOR EARLY DETECTION OF BONE LOSS | 01-14-2016 |
20160046976 | USE OF AT LEAST ONE CHROMOGENIC AND/OR FLUOROGENIC PHOSPHATASE SUBSTRATE FOR THE DETECTION AND/OR ENUMERATION OF ENTEROBACTERIA IN A SAMPLE - Use of at least one chromogenic and/or fluorogenic phosphatase substrate for the detection and/or enumeration of enterobacteria in a sample likely to contain them, such as a food sample. | 02-18-2016 |
20160102335 | BIOLOGICAL INDICATOR - This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed. | 04-14-2016 |
20160145569 | Compositions and Methods Useful for Culturing Differentiable Cells - The present invention relates to cell culture methods and compositions that are essentially serum-free and comprise a basal salt nutrient solution and an ErbB3 ligand. | 05-26-2016 |