| Entries |
| Document | Title | Date |
|---|
| 20080206798 | KITS FOR DETECTION OF ATP - Methods and kits for detecting the presence of ATP, for measuring ATP concentrations, and for detecting viable cells using a composition comprising an ATP-dependent enzyme and one or more ATPase inhibitors. | 08-28-2008 |
| 20080227128 | Resonance Energy Transfer System and Method - The present invention relates to a novel BRET system. The BRET system can be used to identify modified recognition sites within a protein insert and to identify the compounds involved in modulation of a given modification. The BRET system of the present invention can also be used in a screening method construct insert to identify candidates for drug development. | 09-18-2008 |
| 20080227129 | Methods of protein destabilization and uses thereof - This invention is directed towards methods of destabilizing proteins in living cells, and their use for the development of novel assays. In one embodiment, the invention comprises the use of non-cleavable multimerized ubiquitin fusion proteins to destabilize a target protein, such as a reporter moiety. In one aspect of this method the constructs also comprises a linker that operatively couples the reporter moiety to the multimerized ubiquitin fusion protein. In this embodiment, enzymatic modification of the linker results in a modulation of the coupling of the reporter protein to the multimerized ubiquitin domains resulting in a change in the stability of the reporter moiety. The level of the reporter moiety in the cell can then be used as a measure of the enzymatic activity in the cell. In another embodiment the invention provides for a generalized way of coordinately regulating the cellular concentration of a plurality of target proteins. In one aspect of this method, the target proteins are operatively coupled to a ubiquitin fusion protein via a linker containing a protease cleavage site. Cleavage of the linker by a protease results in uncoupling of the target protein from the multimerized ubiquitin construct, and results in an increase in the stability and concentration of the target protein. | 09-18-2008 |
| 20080241865 | VISIBLE TO NEAR-INFRARED LIGHT PROBE COMPRISING COMPLEX OF CYPRIDINA LUCIFERASE AND QUANTUM DOT - The invention relates to a bioactive substance labeled with | 10-02-2008 |
| 20080241866 | Systems and methods for enhancing fluorescent signals - Composition, systems, apparatus and methods of enhancing fluorescent signals in biochemical are described. Metal particle proximity to enzymes that produce fluorescent products provide enhanced fluorescence of the product and plasmon resonance of the metal particle. Multi-labeled nucleotides enhance signal production. Reflectance of illumination light and emitted fluorescence increase signal strength for a given illumination light. | 10-02-2008 |
| 20080248511 | METHODS TO QUENCH LIGHT FROM OPTICAL REACTIONS - The present invention relates to single and dual reporter luminescence assays utilizing reagents to quench an optical, e.g., an enzyme-mediated luminescence, reaction. In one embodiment of the invention, a reagent is added to an assay which selectively quenches a first enzyme-mediated luminescence reaction without affecting a subsequent distinct enzyme-mediated luminescent reaction(s). An assay kit containing one or more selective quench reagents, and compositions comprising the quench reagent(s), are also provided. | 10-09-2008 |
| 20080254491 | Method and Device for Monitoring Aspirin Response - The invention provides a method of monitoring the response of platelets to a COX1 inhibitor such as aspirin. The method involves collecting platelet-containing mammalian blood treated with a COX 1 inhibitor; mixing the blood with a COX 1-dependent platelet agonist, such as arachidonic acid, monitoring extracellular ATP in the agonist-activated bl>>d to generate a measurement, and comparing the measurement to a standard value. Devices, systems, and kits for carrying out the method are also provided. | 10-16-2008 |
| 20080268482 | LUMINOGENIC AND NONLUMINOGENIC MULTIPLEX ASSAY - A method to detect the presence or amount of at least one molecule for an enzyme-mediated reaction in a multiplex luminogenic/nonluminogenic assay is provided. | 10-30-2008 |
| 20080274485 | Enhancing a Luminescent Signal - Methods and compositions are described for assaying luciferase bioluminescence in vitro and in vivo cells. The compositions provide at least one of enhanced stability of signal or magnitude of signal by varying the composition of the buffer. One or more of the following parameters have been varied: the presence or absence of EDTA, the concentration of NaCl, the concentration of coelenterazine, the evaluation of ionic and non-ionic detergent, the amount of detergent, how the detergent has been added and the time over which the signal has been recorded. Also disclosed are dual reporter systems. | 11-06-2008 |
| 20080299592 | Red-Shifted Luciferase - The compositions described herein shift the light output of luciferases to the near-IR by resonance energy transfer to a targetable near-IR fluorophore. | 12-04-2008 |
| 20080299593 | Luminogenic and fluorogenic compounds and methods to detect molecules or conditions - A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided. | 12-04-2008 |
| 20090011446 | COLONY ASSAY MINIATURIZATION WITH ENUMERATION OUTPUT - A system combining a clonogenic differentiation assay with an instrument-based ATP bioluminescence proliferation assay to produce a standardized colony-forming stem and progenitor cell potency assay is provided. | 01-08-2009 |
| 20090029398 | Microbial ATP extraction and detection system - The present invention is directed to compositions and methods for single-step extraction and detection of ATP levels from microbial cells. The disclosed compositions are formulated to efficiently elicit bioluminescent detection of ATP among a broad variety of different microorganisms using a common single-step reagent composition. Additional luminescence-based methods are provided for identifying other useful extracting agents or for screening compounds for their pharmaceutical or biological effects on microbial cells. | 01-29-2009 |
| 20090047693 | Luciferase Detection Assay System - The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method. | 02-19-2009 |
| 20090053744 | Luciferase Detection Assay System - The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method. | 02-26-2009 |
| 20090075308 | Method for in vitro phosphorylation of TRAP of Staphylococcus aureus and a method for screening the inhibitor of the TRAP phosphorylation - The present invention relates to a method for in vitro phosphorylation of TRAP derived from | 03-19-2009 |
| 20090075309 | Bisdeoxycoelenterazine derivatives, methods of use, and BRET2 systems - Embodiments of the present disclosure provide for: compositions, BRET systems, kits, and the like. | 03-19-2009 |
| 20090081715 | Engineered Light-Emitting Reporter Genes - Compositions and methods are provided for enhanced expression of light emitting reporters. Such reporters are used in methods for monitoring cultures for production of target compounds. | 03-26-2009 |
| 20090081716 | Method for Monitoring the Immune Response and Predicting Clinical Outcomes in transplant recipients - Methods for monitoring the immune response and predicting clinical outcomes for patients on immunosuppressive drugs (such as transplant patients) are provided. The methods are based on the measurement of an intracellular metabolic marker in lymphocytes (such as ATP) as an indicator of a patient's immune response. | 03-26-2009 |
| 20090123953 | Double brilliance beta-arrestin: a biosensor for monitoring the activity of receptors and signalling molecules, and method of using same - An intramolecular bioluminescence resonance energy transfer (BRET), biosensor for monitoring receptor activity and signalling cascades is disclosed. The “double-brilliance” biosensor sandwiches β-arrestin (β-arr) between Renilla | 05-14-2009 |
| 20090123954 | MULTICOLOR BIOLUMINESCENT VISUALIZATION PROBE SET, OR SINGLE-MOLECULE-FORMAT MULTICOLOR BIOLUMINESCENT VISUALIZATION PROBE - The present invention provides ligand detection means capable of exhibiting two-dimensional information (wavelength and intensity of luminescent signal) responding to multiple signals triggered by a ligand via a target protein, while taking advantage of the merit of the single-molecule-format bioluminescent probe. | 05-14-2009 |
| 20090136974 | Luciferin Luminescent Substrate of Marine Ostracod Crustacean and Method for Production Thereof - The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): | 05-28-2009 |
| 20090176260 | Double-fusion human embryonic stem cells, methods of making double-fusion human embryonic stem cells, triple-fusion human embryonic stem cells, methods of making triple-fusion human embryonic stem cells, and methods of monitoring double-fusion human embryonic stem cells and triple-fusion human embryonic stem cells - Embodiments of the present disclosure include double-fusion human embryonic stem cells, methods of imaging double-fusion human embryonic stem cells, double-fusion polynucleotides, double-fusion proteins, triple-fusion human embryonic stem cells, methods of imaging triple-fusion human embryonic stem cells, triple-fusion polynucleotides, triple-fusion proteins, methods of monitoring the progression of human embryonic stem cells, methods of making isolated double-fusion human embryonic stem cells, methods of making isolated triple-fusion human embryonic stem cells, and the like. | 07-09-2009 |
| 20090233320 | GENE ENCODING NOVEL LUCIFERASE - The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from | 09-17-2009 |
| 20090239248 | Rapid and sensitive detection of bacteria in blood products, urine, and other fluids - The invention provides methods of detecting bacteria in fluids, including blood, platelets and other blood products for transfusion, and urine. The methods are based on lysing the bacteria to release ATP and detecting the ATP. Eukaryotic cell contamination is a problem to be overcome, because eukaryotic cell contain large amounts of ATP. Thus, some of the methods involve separating intact eukaryotic cells (e.g., platelets) from intact bacterial cells before lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme that catalyzes a reaction, and monitoring the enzyme-catalyzed reaction. Typically, the enzyme is luciferin, and the reaction is monitored by detecting light produced by the luciferin. Other methods of the invention involve contacting a fluid sample with a support surface that binds bacterial cells, lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme, and monitoring the enzyme-catalyzed reaction. Apparatuses for carrying out the methods are also disclosed. | 09-24-2009 |
| 20090275063 | Distinguishing cells in a sample by inactivating extracellular enzyme before releasing intracellular enzyme - A method for detecting the absence or presence of cells of interest in a liquid sample, wherein: (a) the sample: (i) comprises an extracellular medium containing an enzyme with a measurable activity; and (ii) is suspected of containing cells of interest that contain an enzyme with said measurable activity; and (b) the method comprises the steps of: (i) treating the liquid sample with a reagent that inactivates said measurable activity in the extracellular medium, but does not inactivate the measurable activity in said cells of interest; (ii) lysing the cells of interest to release the intracellular enzyme; and (iii) measuring said measurable activity. Thus the intracellular enzyme can be measured without interference from the extracellular enzyme. The invention is particularly useful for treatment of bacterially-infected blood using a detection assay based on adenylate kinase activity. | 11-05-2009 |
| 20090291463 | IMPLEMENTATION OF MICROFLUIDIC COMPONENTS, INCLUDING MOLECULAR FRACTIONATION DEVICES, IN A MICROFLUIDIC SYSTEM - A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample. | 11-26-2009 |
| 20090298102 | Cell Potency Assay - Cell potency assays for use with cell-based therapies, and specifically, with stem cell therapies, are provided. Cell potency assays for bone marrow cells, mobilized peripheral blood, and umbilical cord blood are provided. | 12-03-2009 |
| 20100009395 | LUMINESCENCE ASSAY UTILIZING A GENETICALLY MODIFIED CELL LINE - An assay method to identify agents that will reduce the inflammation associated with many diseases, providing a method to determine compliance of patients to clinical protocols. The fundamental tool of the inventive method is luminescence. Genetically modified cells are used to express a complex revealing the potential of certain compounds to prevent or reduce adverse effects. More specifically the invention is a method for the determination of inhibition of a chemical compound comprising: culturing genetically modified cells which express an indicator-luminescent complex; placing said complex in the presence of an agent that essentially totally degrades said complex; measuring the luminescence of the resulting reaction; collecting a sample from a mammal consuming a complementary and alternative medicine (CAM); placing said sample in the presence of said complex; and comparing the level of luminescence from step (c) with the luminescence from step (e) to determine the inhibition of said chemical compound. | 01-14-2010 |
| 20100035287 | Secreted Luciferase Fluorescent Protein Conjugate Nucleic Acid Construct and Uses Thereof - The present invention relates generally to methods to monitor the transport of proteins through the secretory pathway, and methods to monitor ER stress. In particular, the present invention relates to methods to monitor, in real-time, the processing of protein through the secretory pathway, which can be monitored both at a subcellular level by florescence visualization and quantitatively by detecting the secreted luciferase reporter protein. The present invention also relates to methods to assess biological processes in cells, in particular the secretory pathway and ER stress, as well as methods to identify agents which augment or inhibit the secretory pathway and/or ER stress. The present invention also relates to compositions and nucleic constructs encoding a secreted luciferase-fluorescent protein conjugate for methods to monitor protein trafficking in the cell by simultaneous detection of fluorescence and luciferase secretion. | 02-11-2010 |
| 20100068739 | PH Tolerant Luciferase - Use of a luciferase that has a mutation of at least one amino acid selected from the group consisting of positions 14, 35, 182, 232 and 465, where the numbering is according to the sequence of the luciferase from | 03-18-2010 |
| 20100075350 | ADP detection based luminescent phosphotransferase or ATP hydrolase assay - The invention provides compositions and methods to determine or detect the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP. | 03-25-2010 |
| 20100075351 | BIOLUMINESCENT DETECTION OF CYANOHYDROXY BENZOTHIAZOLE COMPOUNDS - The invention provides methods that employ derivatives of 2-cyano-6-hydroxy- or 2-cyano-6-amino-benzothiazole, for example, in a bioluminogenic reaction. Also provided are novel compounds that can be used in the methods. The invention further provides methods for detecting or determining the presence of molecules and/or enzymes, the modulator activity of such molecules, and/or the activity of such enzymes. The methods are adaptable to high-throughput format. | 03-25-2010 |
| 20100105090 | SECRETED LUCIFERASE MLUC7 AND USE THEREOF - The invention relates to the nucleotide and amino acid sequences and to the activity and use of the secreted MLuc7 luciferase. | 04-29-2010 |
| 20100112611 | PROCEDURE FOR DETECTING MICROBIAL CONTAMINATION BY BIOLUMINESCENCE IN ASSOCIATIVE ACRYLIC THICKENERS AND PRODUCTS CONTAINING THEM - A procedure for detecting microorganisms by bioluminescence in an aqueous formulation containing an ASE or HASE-type polymer, which implements at least one step of dilution of the aqueous formulation. This dilution step, and notably the regulation of the dilution factor, allows the bioluminescence technique, up to now ineffective on this type of products, to be implemented. It can henceforth be used on these ASE or HASE-type polymers, but also on products containing them, such as a paper coating, paint, lacquer, varnish or stain. | 05-06-2010 |
| 20100120075 | Composition and a method for suppressing the luminescence intensity decline in a luciferin-luciferase reaction - The invention relates to compositions and methods for suppressing the luminescence intensity decline that occurs as the reaction between | 05-13-2010 |
| 20100129840 | Method and Apparatus for Reducing Luminescent Test Result Interferences - This application involves detecting luminescence. Various methods and devices are described that reduce interference of ambient light, including UV radiation, on test results. Such methods and devices include using UV blocking material and covering test components prior to use. | 05-27-2010 |
| 20100173335 | Fast Microbiological Analysis Device And Method - The device includes means for holding a support ( | 07-08-2010 |
| 20100203561 | METHOD FOR USING DIVISION ARRESTED CELLS IN SCREENING ASSAYS - Division arrested cells are used in screening assays to determine the effect of a substance of interest on the cells. The division arrested cells can be used in drug screening assays, signal transduction assays, and are especially useful in large scale, high throughput assays. | 08-12-2010 |
| 20100209949 | LUMINESCENCE MEASUREMENT METHOD AND LUMINESCENCE MEASUREMENT SYSTEM - Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample. | 08-19-2010 |
| 20100227344 | METHODS FOR MEASURING ADP - This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers. | 09-09-2010 |
| 20100233742 | Method of Quantifying Autoinducer-2 - A method of quantifying autoinducer-2, including the steps of:
| 09-16-2010 |
| 20110020849 | BIOMARKERS FOR DIAGNOSTIC AND THERAPEUTIC METHODS - Erythrocyte ATP-release modulators and composition and methods for their use as biomarkers of glucose processing or vascular disorders, as well as methods for screening to identify to modulators; methods for monitoring efficacy of therapy; and apparatus for use in such methods. | 01-27-2011 |
| 20110027814 | BRCA1 function-based cellular assays - Assays using binding studies involving function of BRCA1a protein have use for diagnosis and for evaluation of possible tumorogenicity of agents, particularly estrogenic agents. The assays do not rely on use of a probe for only specific sequences, but on effects of known and unknown or not previously studied sequences (consequence of genetic changes) or posttranslational modification of BRCA1 proteins (as a consequence of epigenetic changes) as seen in hereditary and sporadic cancers. | 02-03-2011 |
| 20110033878 | LUMINESCENT SUBSTRATE FOR LICIFERASE - The present invention relates to a compound having a structure analogous to firefly luciferin. In particular, the invention relates to a heterocycle compound which produces a luminescence at a light wavelength different from that of firefly luciferin in nature. The present invention provides a heterocycle compound of following general formula I. In the above general formula, R | 02-10-2011 |
| 20110076706 | METHODS AND KITS FOR THE RAPID DETECTION OF MICROORGANISMS - The present invention provides analytical methods and related kits for detecting the presence of bacteria in a sample, determining the amount of bacteria in a sample, or determining the type of bacteria in a sample. In particular, the invention relates to devices and methods suitable for the rapid detection, quantification, or identification of bacteria in a liquid sample by measuring bacterial ATP activity. | 03-31-2011 |
| 20110081670 | LUCIFERASE-BASED ASSAYS - A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent. | 04-07-2011 |
| 20110091916 | LUCIFERIN-LUCIFERASE BASED MICRODEVICE FOR BIOSENSING - A method and apparatus for determining the concentration of one or more microbes in a sample is provided. This method involves filtering a sample through a filter inside a sample tube to retain the one or more microbes on the filter. The resulting filtrate, which contains or produces adenosine triphosphate, is passed through the sample tube and enters the reporter region. In the reporter region, the adenosine triphosphate in the filtrate comes in contact with a transparent porous matrix, which includes a luciferin-luciferase complex. The adenosine triphosphate interacts with the luciferin-luciferase complex to provide light response, which is measured by a detector. The light response is compared with a calibration curve to determine the total concentration of one or more microbes in a sample. | 04-21-2011 |
| 20110097753 | Chemiluminescent methods and reagents for analyte detection - The present invention relates to chemiluminescent method and regent to detect analyte. One aspect of the current invention relates to using enzyme substrate that can be cleaved by target enzyme to release chemiluminescent compound giving light signal for the detection of varieties of target enzymes. Another aspect of the current invention relates to use chemiluminescent enzyme coupled with analyte binding molecules to detect specific analyte molecules in a homogenous phase. | 04-28-2011 |
| 20110111441 | METHOD AND KIT FOR MEASUREMENT OF ENDOTOXIN LEVEL - The present invention provides a method comprising allowing a reaction of a sample, a reagent containing Factor C, which can be activated by binding with endotoxin, and a synthetic luminescent substrate comprising a luminescent substrate bound to a peptide, for release of the luminescent substrate from the synthetic luminescent substrate, allowing a luminescent enzyme to act on the luminescent substrate released in the luminescent substrate release step, for measurement of the luminescence intensity, and quantifying the level of endotoxin in the sample based on a measured value obtained in the luminescence measuring step, the method enabling endotoxin to be simply and quickly measured at a level that cannot be detected in conventional methods for endotoxin measurement, without use of any dedicated measuring device. | 05-12-2011 |
| 20110136156 | LUCIFERINS - Novel luciferins, methods of making luciferins, and uses of the same are disclosed. | 06-09-2011 |
| 20110159529 | FUSION PROTEIN HAVING LUMINESCENCE ACTIVITY - The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes. | 06-30-2011 |
| 20110171669 | Isolated luciferase gene of L. ITALICA - The present invention relates to an isolated nucleic acid and polypeptide sequence that encodes for a luciferase of | 07-14-2011 |
| 20110171670 | REAGENT OPEN MECHANISM OF LUMINESCENCE MEASUREMENT SYSTEM AND OPEN NEEDLE CONTROL METHOD IN REAGENT OPEN MECHANISM - A reagent open mechanism of the luminescence measurement system comprises a triaxial actuator and a reagent dispensing nozzle which is driven by the triaxial actuator. A reagent cartridge where a reagent to be divided by the reagent dispensing nozzle is filled in a concave and the opening of the concave is sealed by an aluminum sheet can be set in. This reagent open mechanism comprises an open needle which is driven by the triaxial actuator and makes a hole in the aluminum sheet and a fixation block between the reagent dispensing nozzle and the open needle which arranges the reagent dispensing nozzle and the open needle in such location that the reagent dispensing nozzle or the open needle does not contact with a structure including the reagent cartridge in a Z-axis operation during opening time or reagent dividing and dispensing time. | 07-14-2011 |
| 20110177539 | COVALENTLY LINKED THERMOSTABLE KINASE FOR DECONTAMINATION PROCESS VALIDATION - A biological process indicator is provided for validating a treatment process in which the amount or activity of a contaminant in a sample is reduced. The indicator comprises a thermostable kinase covalently linked to a biological component, with the proviso that the biological component is not an antibody. Methods of preparing the indicator, and methods of using the indicator, are also provided. | 07-21-2011 |
| 20110177540 | MUTANT LUCIFERASE - An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from | 07-21-2011 |
| 20110223625 | BIOLUMINESCENT ASSAYS USING CYANOBENZOTHIAZOLE COMPOUNDS - The invention provides methods that employ derivatives of 2-cyano-6-hydroxy- or 2-cyano-6-amino-benzothiazole, for example, in a bioluminogenic reaction. The invention further provides methods for detecting or determining the presence of molecules and/or enzymes, the modulator activity of such molecules, and/or the activity of such enzymes. The methods are adaptable to high-throughput format. | 09-15-2011 |
| 20110256564 | METHODS, REAGENTS AND KITS FOR LUCIFERASE ASSAY - The invention relates to methods, reagents and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel luciferase assay system with reduced background luminescence to allow for increased detection sensitivity. Provided is a method of detecting luciferase activity in a sample using coelenterazine or an analog thereof as a substrate, comprising: (a) initiating luciferase-catalyzed luminescence production by contacting said sample with a luciferase detection reagent to yield a reaction mixture, said reagent comprising coelenterazine and at least one iodide source in an amount sufficient to reduce the autoluminescence of said coelenterazine, (b) incubating said reagent mixture under conditions suitable to produce luminescence, and (c) measuring the luminescence produced. Also provided are detections reagents and kits for use in such a method. | 10-20-2011 |
| 20110275101 | METHOD FOR USING DIVISION ARRESTED CELLS IN SCREENING ASSAYS - Division arrested cells are used in screening assays to determine the effect of a substance of interest on the cells. The division arrested cells can be used in drug screening assays, signal transduction assays, and are especially useful in large scale, high throughput assays. | 11-10-2011 |
| 20110281286 | EXPRESSION VECTOR FOR ESTABLISHING HIGHLY PRODUCTIVE CELL AND THE HIGHLY PRODUCTIVE CELL - The present invention provides an expression vector which is effective in an efficient establishment of transformed cells which express the aimed protein gene in a high level. An expression vector which has a cassette for expressing the drug selective marker gene containing mRNA destabilizing sequence, at least one element for stabilizing the gene expression and a cassette for expressing the gene of the aimed protein. Preferably, the mRNA destabilizing sequence is derived from AT-rich sequence existing in the 3′-untranslated region of cytokine, interleukin or proto-oncogene, and the element for stabilizing the gene expression is derived from Chinese hamster genome. | 11-17-2011 |
| 20110287459 | Luciferin luminescent substrate of marine ostracod crustacean and method for production thereof - The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): | 11-24-2011 |
| 20110312001 | COMPENDIUM OF READY-BUILT STEM CELL MODELS FOR INTERROGATION OF BIOLOGICAL RESPONSE - The invention generally features methods for providing engineered pluripotent stem cells that can be used to study biological response and pathways, including differentiation and drug effects. For example, these cells are provided comprising two or more exogenous expression cassettes including a selectable or screenable marker under the control of different condition-responsive regulatory elements, such as differentiation-responsive promoters or regulatory element of a receptor, drug target, drug metabolizing enzyme or signaling pathway gene. Also provided are sets of stem cell lines each comprising a different exogenous expression cassette including a selectable or screenable marker under the control of a different condition-responsive regulatory element. | 12-22-2011 |
| 20120034634 | MODIFIED LUCIOLA CRUCIATA LUCIFERASE PROTEIN - A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from | 02-09-2012 |
| 20120034635 | METHOD FOR AMPLIFYING ADENOSINE TRIPHOSPHATE AND METHOD AND REAGENT FOR DETECTING THE CONCENTRATION OF MICROORGANISMS - A method for amplifying adenosine triphosphate is provided, including mixing adenosine triphosphate sulfurylase, adenosine 5′ phosphosulfate, adenylate kinase, uridine triphosphate, acetate kinase, acetyl phosphate, luciferin and luciferase in the presence of ATP to form a mixture, and reacting the mixture to amplify ATP. A method and reagent for detecting a concentration of microorganisms are also provided. | 02-09-2012 |
| 20120058499 | Bioluminescent Detection of Protease Activity - Methods for bioluminescent detection of the activity of proteolytic enzymes including ubiquitin (Ub) and ubiquitin-like (Ubl) proteolytic enzymes are disclosed. | 03-08-2012 |
| 20120107849 | COELENTERAZINE DERIVATIVES AND METHODS OF USING SAME - The invention provides coelenterazine derivatives which are substrates for a non-luminescent enzyme and a pro-substrate for a luminescent enzyme. The invention also provides a method of using the derivatives. | 05-03-2012 |
| 20120135435 | Methods for Identifying Factors That Control the Folding of Amyloid Proteins of Diverse Origin - The present invention provides a yeast cell based system for determining factors that control the folding of amyloid proteins of diverse origins. Further the present invention provides methods of using such a system to screen for reagents that affect amyloid formation, a process that is integral to several devastating human disease including Creutzfeld-Jacob disease (CJD), fatal familial insomnia (FFI), Gertsmann-Straussler-Scheinker (GSS) syndrome, and kuru. The system of the present invention provides a rapid screening system to quickly and cheaply identify reagents that affect the folding and aggregation properties of the target protein. | 05-31-2012 |
| 20120149045 | LUMINESCENT LIVE AND DEAD CELL ASSAY - A method to detect live and dead cells in a sample with one bioluminogenic reagent is provided. | 06-14-2012 |
| 20120149046 | Bioluminescent Bacterial Detection - This invention relates to bioluminescent methods for detecting specific target bacteria, such as | 06-14-2012 |
| 20120156705 | LUCIFERASES AND USES THEREOF - The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases with different emission spectra. | 06-21-2012 |
| 20120231483 | METHOD AND APPARATUS FOR REDUCING LUMINESCENT TEST RESULT INTEREFERENCES (CONTINUATION) - This application involves detecting luminescence. Various methods and devices are described that reduce interference of ambient light, including UV radiation, on test results. Such methods and devices include using UV blocking material and covering test components prior to use. | 09-13-2012 |
| 20120252042 | Artificial Feces - A composition to simulate feces. A method of using artificial feces to evaluate the efficacy of a cleaning product and/or cleaning procedure. A method of demonstrating the cleaning efficiency of a cleaning product and/or cleaning procedure using artificial feces. | 10-04-2012 |
| 20120258480 | ADP DETECTION BASED LUMINESCENT PHOSPHOTRANSFERASE OR ATP HYDROLASE ASSAY - The invention provides provides compositions and methods to determine or detect the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP. | 10-11-2012 |
| 20120270247 | LUMINESCENCE MEASUREMENT METHOD AND LUMINESCENCE MEASUREMENT SYSTEM - Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample. | 10-25-2012 |
| 20120276563 | METHOD FOR SCREENING A POTENTIAL MODULATOR COMPOUND OF A TASTE RECEPTOR - A method for screening a potential modulator compound of a taste receptor wherein use is made of a BRET technique. | 11-01-2012 |
| 20120276564 | Luciferins - Novel luciferins, methods of making luciferins, and uses of the same are disclosed. | 11-01-2012 |
| 20120282639 | LIGHT COLLECTION SYSTEM - The present teachings provide a detection cell for a biological material and methods for detecting biological material including a photosensitive material optically coupled to an interior volume containing the biological material so to avoid optical components or an external light source. | 11-08-2012 |
| 20120301907 | METHODS, DEVICES, AND SYSTEMS OF DETECTING MICROORGANISMS - A rapid, sensitive method of separating and detecting microorganisms from a sample potentially containing microorganisms, such as but not limited to bacteria, fungi, yeast, viruses, and the like. The method relies on separation techniques to separate and concentrate the cells from the sample, together with chemical techniques to amplify the amount of detectable signal from low numbers of cells to provide a rapid and sensitive method of detecting microorganisms. This detection method may utilize: a filtration device; a centrifugation device; a system; a swab device; and kit comprising one or more of the devices and components to perform the present method of separating and detecting microorganisms in a sample potentially containing microorganisms. The sample may be a chemical, cosmetic, personal care, pharmaceutical, or consumable good in its raw material, in-process, and/or finished product states that needs to be tested for any contaminating microorganisms prior to shipment to the consumer. | 11-29-2012 |
| 20120315657 | FUNCTIONAL ASSAY FOR 5-HT2A, HISTAMINE H1 OR ADRENERGIC ALPHA 1B RECEPTORS - The present invention provides novel functional assay for 5-HT | 12-13-2012 |
| 20120329081 | SAMPLING DEVICES AND METHODS OF USE - Sample-collecting devices, articles and methods of use are disclosed. Premoistened sample-collecting devices comprise 1,2-propanediol as a humectant, which promotes the retention of a liquid solution on the sample-collecting device during storage. 1,2-propanediol as humectant can be compatible with proteins and other reagents used to detect an analyte. | 12-27-2012 |
| 20130017564 | BIOPRINTING STATION, ASSEMBLY COMPRISING SUCH BIOPRINTING STATION AND BIOPRINTING METHODAANM Guillemot; FabienAACI BordeauxAACO FRAAGP Guillemot; Fabien Bordeaux FRAANM Catros; SylvainAACI BordeauxAACO FRAAGP Catros; Sylvain Bordeaux FRAANM Keriquel; VirginieAACI BordeauxAACO FRAAGP Keriquel; Virginie Bordeaux FRAANM Fricain; Jean-ChristopheAACI BordeauxAACO FRAAGP Fricain; Jean-Christophe Bordeaux FR - Bioprinting station (1) comprising:—a Bioprinting device (4) adapted to deposit a pattern of biological material (2) onto an area of interest (3 | 01-17-2013 |
| 20130022999 | PROTEIN FRAGMENT COMPLEMENTATION ASSAYS FOR THE DETECTION OF BIOLOGICAL OR DRUG INTERACTIONS - The present invention describes a method for detecting biomolecular interactions said method comprising: (a) selecting an appropriate reporter molecule selected from the group consisting of a protein, a fluorescent protein, a luminescent protein and a phosphorescent protein; (b) effecting fragmentation of said reporter molecule such that said fragmentation results in reversible loss of reporter function; (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments; and (e) detecting said biomolecular interactions by reconstitution of activity of the reporter molecule | 01-24-2013 |
| 20130040326 | FIREFLY LUCIFERASE - According to one embodiment, the present invention relates to luciferase derived from Malaysian | 02-14-2013 |
| 20130045496 | Automatic Fluid Sample Preparation Module, Automatic Analysis System and Method for Use Thereof - This present invention relates to an automatic fluid sample preparation module and method for use thereof, for automatically preparing a fluid sample for detecting micro-organisms and/or biological substances. The invention further relates to an automatic analysis system. The automatic fluid sample preparation module comprises:—an inlet for automatically obtaining a sample directly from a fluid or fluid stream to be analyzed; —preparation means for preparing the fluid sample from the sample, comprising first coupling means for coupling with the inlet; and—an outlet for discharge of prepared fluid to measurement means, wherein the preparation means are provided such that micro-organisms and/or biological substances from one or multiple samples are accumulated in the prepared fluid sample. | 02-21-2013 |
| 20130045497 | DETECTION OF HYDROGEN PEROXIDE - The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same. | 02-21-2013 |
| 20130065259 | METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN - Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples. | 03-14-2013 |
| 20130071866 | METHOD FOR TESTING THE SEVERTIY OF AN ILLNESS - An object of the present invention is directed to a method for assaying the severity of an illness in real time and is to provide a testing method capable of assessing the severity of an illness in more detail than the conventional APACHE II and SOFA scores. The established method can accurately measure an ATP level in a sample, thereby accurately and quickly deducing the “state of intracellular energy required for living organisms” from the ATP level, and by extension, determining the severity of an illness. The present invention further provides a novel biomarker ATP-lactate energy risk score (A-LES) value that is capable of determining the severity of an illness by the reevaluation, with the ATP concentration as an index (specifically, on the basis of a lactic acid level (mM)/ATP concentration (mM) ratio), of the level of lactic acid that accumulates in the sample due to the breakdown of in vivo metabolic balance accompanied by the increased severity of the illness. The present invention also provides a novel biomarker ATP-ketone energy risk score (A-KES) value that is capable of determining the severity of an illness by the reevaluation of a ketone body level in the sample with the ATP concentration as an index (specifically, on the basis of a ketone body level (mM)/ATP concentration (mM) ratio). | 03-21-2013 |
| 20130078658 | METHOD OF QUANTIFYING RECOVERY RATE OF EXOSOME - A recombinant exosome comprising a fusion protein of a membrane protein and light-emitting protein, and a method of determining an exosome recovery rate by using the recombinant exosome are provided. Use of the method ensures accurate quantification of exosomes in a sample, and thus, improves the efficiency of an exosome-based diagnosis. | 03-28-2013 |
| 20130084588 | RAPID AND SENSITIVE DETECTION OF BACTERIA IN BLOOD PRODUCTS, URINE, AND OTHER FLUIDS - The invention provides methods of detecting bacteria in fluids, including blood, platelets and other blood products for transfusion, and urine. The methods are based on lysing the bacteria to release ATP and detecting the ATP. Eukaryotic cell contamination is a problem to be overcome, because eukaryotic cell contain large amounts of ATP. Thus, some of the methods involve separating intact eukaryotic cells (e.g., platelets) from intact bacterial cells before lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme that catalyzes a reaction, and monitoring the enzyme-catalyzed reaction. Typically, the enzyme is luciferin, and the reaction is monitored by detecting light produced by the luciferin. Other methods of the invention involve contacting a fluid sample with a support surface that binds bacterial cells, lysing the bacterial cells to release ATP, contacting the ATP with an ATP-consuming enzyme, and monitoring the enzyme-catalyzed reaction. Apparatuses for carrying out the methods are also disclosed. | 04-04-2013 |
| 20130095509 | ENGINEERED LIGHT-EMITTING REPORTER GENES - Compositions and methods are provided for enhanced expression of light emitting reporters. Such reporters are used in methods for monitoring cultures for production of target compounds. | 04-18-2013 |
| 20130109037 | METHODS FOR DETECTING ADENOSINE MONOPHOSPHATE IN BIOLOGICAL SAMPLES | 05-02-2013 |
| 20130115641 | ISOLATED LUCIFERASES AND THE USE THEREOF - The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22. | 05-09-2013 |
| 20130130289 | COMPOUNDS AND METHODS FOR ASSAYING REDOX STATE OF METABOLICALLY ACTIVE CELLS AND METHODS FOR MEASURING NAD(P)/NAD(P)H - The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement. | 05-23-2013 |
| 20130130290 | DISTINGUISHING CELLS IN A SAMPLE BY INACTIVATING EXTRACELLULAR ENZYME BEFORE RELEASING INTRACELLULAR ENZYME - A method for detecting the absence or presence of cells of interest in a liquid sample, wherein: (a) the sample: (i) comprises an extracellular medium containing an enzyme with a measurable activity; and (ii) is suspected of containing cells of interest that contain an enzyme with said measurable activity; and (b) the method comprises the steps of: (i) treating the liquid sample with a reagent that inactivates said measurable activity in the extracellular medium, but does not inactivate the measurable activity in said cells of interest; (ii) lysing the cells of interest to release the intracellular enzyme; and (iii) measuring said measurable activity. Thus the intracellular enzyme can be measured without interference from the extracellular enzyme. The invention is particularly useful for treatment of bacterially-infected blood using a detection assay based on adenylate kinase activity. | 05-23-2013 |
| 20130189717 | ASSAY METHOD FOR THE DETECTION OF VIABLE MICROBIAL CELLS IN A SAMPLE - The present invention discloses an assay method for the detection of viable microbial cells in a sample, the assay method comprising the steps of: i) adding an ATP degrading enzyme to a sample suspected of containing viable microbial cells to substantially degrade any extracellular ATP in the sample; ii) adding a phosphate containing compound to the sample to substantially halt action of the ATP degrading enzyme; and iii) subjecting the sample to a detection assay to establish the level of undegraded ATP in the sample to provide an indication of the level of viable microbial cells in the sample. | 07-25-2013 |
| 20130196357 | COMPOSITIONS AND METHODS FOR MONITORING TRANSMEMBRANE TRAFFICKING - Provided herein are compositions and methods for monitoring the movement of analytes and/or cellular components across biological membranes (e.g., cell surface internalization). In particular, reporter constructs are provided, the transmembrane movement of which (e.g., by endocytosis) is monitored by methods described herein. | 08-01-2013 |
| 20130217051 | LUCIFERASE-BASED ASSAYS - A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent. | 08-22-2013 |
| 20130217052 | LUCIFERASE-BASED ASSAYS - A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement agent. | 08-22-2013 |
| 20130230872 | PLASMID VECTOR, METHOD FOR DETECTING GENE PROMOTER ACTIVITY, AND ASSAY KIT - According to one embodiment, a first gene encodes a reporter protein. The first gene is disposed at the downstream of the gene promoter. A second gene is disposed at the downstream of the gene promoter and encodes a replication origin-binding protein. An internal ribosome entry site is disposed between the first gene and the second gene. The transcription termination signal sequence encodes a signal for terminating the transcription of the first gene and the second gene. A replication origin sequence is recognized by the replication origin-binding protein. | 09-05-2013 |
| 20130273582 | LIGHT-EMITTING MOLECULES - Disclosed are luciferase polypeptides with improved light-emitting activity and their encoding nucleic acids. These molecules are useful in a range of assays including luciferase-based gene reporter assays, bioluminescence resonance energy transfer assays, protein complementation assays and other applications in which luciferase enzymes are utilized as detectable and/or quantifiable labels. Also disclosed are methods and compositions for increasing the sensitivity and/or improving the kinetics of luciferase-catalyzed reactions as well as decreasing the impact of undesirable variables. | 10-17-2013 |
| 20130280741 | METHOD AND SYSTEM OF DETECTING DIOXIN-LIKE COMPOUNDS - The invention relates to a method or a cell free system of detecting dioxin-like compounds in a test sample using a whole cell lysate derived from a cell transfected to express a fusion protein comprising an AHR fused to a reporter peptide. The method in combination with bioluminescence resonance energy transfer (BRET) technique is also provided so as to improve the sensitivity. | 10-24-2013 |
| 20130309700 | METHODS, DEVICES, AND SYSTEMS OF DETECTING MICROORGANISMS - A rapid, sensitive method of separating and detecting microorganisms from a sample containing microorganisms, such as but not limited to bacteria, fungi, yeast, viruses, and the like. The method relies on separation techniques to separate and concentrate the cells from the sample, together with chemical techniques to amplify the amount of detectable signal from low numbers of cells to provide a rapid and sensitive method of detecting microorganisms. This detection method may utilize: a filtration device; a centrifugation device; a system; a swab device; and kit comprising one or more of the devices and components to perform the present method of separating and detecting microorganisms in a sample containing microorganisms. The sample may be a chemical, cosmetic, personal care, pharmaceutical, or consumable good in its raw material, in-process, and/or finished product states that needs to be tested for any contaminating microorganisms prior to shipment to the consumer. | 11-21-2013 |
| 20130337480 | CHEMILUMINESCENCE-BASED HAEMOSTASIS ASSAY - The present invention relates to a method for in vitro determining generation of a haemostatis factor such as thrombin and/or plasmin in a test sample using a chemiluminescent substrate specific for said blood clotting factor. Upon cleavage of the substrate, a luminescent signal is generated via aminoluceferin with the aid of a luciferase. The invention also relates to a kit for in vitro determining generation of a haemostasis factor in a test sample, and to novel chemiluminescent substrates for the determination of thrombin and plasmin. | 12-19-2013 |
| 20140024058 | COELENTERAZINE ANALOGS AND MANUFACTURING METHOD THEREOF - There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1). | 01-23-2014 |
| 20140030746 | KINASE SUBSTRATE SENSOR - The disclosure relates to a cytoplasmic protein complex comprising: (a) a first recombinant fusion protein comprising a kinase, fused to a first interaction polypeptide; and (b) a second recombinant fusion protein comprising a domain comprising a reporter phosphorylation site, whereby the domain is fused to a second interaction polypeptide. The disclosure relates further to a method to detect compound-compound-interaction using the cytoplasmic protein complex, and to cells comprising such cytoplasmic protein complex. | 01-30-2014 |
| 20140051101 | Luciferase Reporter System for Roots and Methods of Using the Same - The present invention relates to a systems and methods of using expression of one or two luminescent proteins in a plant root cell to visualize plant root structure as well as to determine how stressors affect gene expression in plant roots while maintaining the natural soil habitat | 02-20-2014 |
| 20140080159 | LIGHT COLLECTION SYSTEM - The present teachings provide a detection cell for a biological material and methods for detecting biological material including a photosensitive material optically coupled to an interior volume containing the biological material so to avoid optical components or an external light source. | 03-20-2014 |
| 20140087402 | Synthetic luciferase gene and protein - A codon optimized and stabilized luciferase gene and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described. | 03-27-2014 |
| 20140093894 | COMPOUNDS AND METHODS FOR ASSAYING REDOX STATE OF METABOLICALLY ACTIVE CELLS AND METHODS FOR MEASURING NAD(P)/NAD(P)H - The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement. | 04-03-2014 |
| 20140099654 | REAL-TIME MONITORING - Provided herein are methods for the real-time monitoring of an intracellular event or response. In particular, the methods provided herein monitor the conversion of a pro-substrate to a substrate for a protein sensor as a result of an intracellular event or response. | 04-10-2014 |
| 20140154716 | LUMINOGENIC AND FLUOROGENIC COMPOUNDS AND METHODS TO DETECT MOLECULES OR CONDITIONS - A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided. | 06-05-2014 |
| 20140170686 | MUTANT LUCIFERASE - An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from | 06-19-2014 |
| 20140170687 | MUTANT LUCIFERASE - An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from | 06-19-2014 |
| 20140186864 | Method for Identifying a Malodor Inhibitor - Provided is a method for identifying a malodor inhibitor based on a response of an olfactory receptor. The present invention provides a method for identifying a malodor inhibitor including: adding a test substance and a malodor-causing substance to at least one olfactory receptor selected from the group consisting of OR5P3, OR5K1, OR2W1, OR8H1, and a polypeptide which has 80% or more identity in amino acid sequence to any one of the aforementioned polypeptides; measuring the response of the olfactory receptor to the malodor-causing substance; identifying the test substance which can suppress the response of the olfactory receptor based on the measured response; and selecting, as a malodor inhibitor, the test substance which can suppress the response of the olfactory receptor. | 07-03-2014 |
| 20140199715 | LIGHT COLLECTION SYSTEM - The present teachings provide a detection cell for a biological material and methods for detecting biological material including a photosensitive material optically coupled to an interior volume containing the biological material so to avoid optical components or an external light source. | 07-17-2014 |
| 20140273036 | LUMINESCENT DETECTION OF INORGANIC PHOSPHATE AND COUPLED REACTIONS - Luminescent detection of inorganic phosphate is carried out in an assay through the intermediary enzymatic production of ADP. ADP is converted to ATP which is used in a luminescent reaction. The assay can be used to monitor coupled enzyme reactions which use or generate inorganic phosphate and the modulation of such reactions. | 09-18-2014 |
| 20140273037 | COMPOSITIONS AND METHODS DIRECTED TO CRISPR/CAS GENOMIC ENGINEERING SYSTEMS - The invention relates to engineered CRISPR/Cas9 systems for genomic modification in mammalian cells. The present specification describes the design and testing of a polynucleotide encoding the | 09-18-2014 |
| 20140273038 | BIOLUMINESCENT METAL ION ASSAY - A method for determining metal ions, both qualitatively and quantitatively, is disclosed. The method utilizes emission from fluorescence resonance energy transfer from a luciferase-carbonic anhydrase conjugate or fusion protein to an acceptor ligand in the presence of metal ion bound to the protein to measure free metal ion concentrations down to picomolar concentration ranges. The method is relatively insensitive to contaminants, and so can be used to measure metal ion concentrations in cells, body fluids or environmental samples without extensive sample preparation. | 09-18-2014 |
| 20140273039 | ASSAY FOR CLOSTRIDIUM BOTULINUM NEUROTOXIN - The present invention relates to a method of determining Botulinum toxin (BoNT) based on a luminescence assay. The present application further relates to a peptide that is susceptible to proteolytic cleavage by BoNT which is suitable for that method. | 09-18-2014 |
| 20140302539 | COELENTERAZINE ANALOGS - Coelenterazine analogs having luminescence properties different from those of known coelenterazine analogs are desired for various luciferases. The invention provides compounds represented by general formula (1) below. | 10-09-2014 |
| 20140329257 | DETECTION OF HYDROGEN PEROXIDE - The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same. | 11-06-2014 |
| 20150010931 | N-END RULE PROTEASE ACTIVITY INDICATION METHODS AND USES THEREOF - A cell based assay for detection for protease activity is disclosed. In the assay a cell is engineered to express a protease substrate with at least one label, preferably on its C-terminus. Cleavage of the substrate by the protease that recognizes it results in a C-terminal fragment and a N-terminal fragment, where the fragment having the label is subject to ubiquitin proteasome degradation. The assay measures the disappearance of the label due to degradation of the fragment to which it is attached. A cell free assay is also described for detection of protease activity. In the cell free assay, the protease substrate is expressed in a solution that includes the elements of the ubiquitin proteasome pathway for degradation of the fragment. The assay measures the disappearance of the label attached to the fragment that results from cleavage by the protease. | 01-08-2015 |
| 20150010932 | METHODS FOR ASSAYING PROTEIN-PROTEIN INTERACTIONS - Provided herein is an assay for interrogating transient and dynamic protein-protein interactions and for screening and characterizing agents as agonists or antagonists of protein-protein interactions. The methods can provide a single assay for simultaneously assessing the bioavailability and efficacy of a test compound for increasing or decreasing a protein-protein interaction of interest. | 01-08-2015 |
| 20150010933 | Novel Screening Methods - The present invention includes a luciferase-based high-throughput screening assay that identifies compounds that are inhibitors of cellular metabolism. This assay is applicable to all bacterial and eukaryotic membranes. | 01-08-2015 |
| 20150044709 | MEANS AND METHODS FOR DETERMINING NEUROTOXIN ACTIVITY BASED ON A MODIFIED LUCIFERASE - The present invention is concerned with test systems for determining the activity of neurotoxin polypeptides. Specifically, it relates to a polynucleotide encoding a single chain luciferase fusion polypeptide comprising: (i) a LuxB subunit, (ii) a linker comprising a neurotoxin cleavage site, and (iii) a LuxA subunit and a polypeptide encoded by the polynucleotide. Further provided in accordance with the invention are a vector and a host cell comprising the polynucleotide. Moreover, the present invention relates to a method for determining a proteolytically active neurotoxin polypeptide in a sample and a kit for carrying out the method. | 02-12-2015 |
| 20150044710 | ENZYMATIC SENSORS AND METHODS FOR THEIR PREPARATION AND USE - Disclosed herein are methods, compositions and devices for detecting oxygen in various samples such as environmental and biological samples. | 02-12-2015 |
| 20150064731 | OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE - The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives according for Formula (Ia) or Formula (Ib). The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules. | 03-05-2015 |
| 20150111233 | MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF - Luciferases which are different from those known heretofore have been desired. A luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from the group consisting of valine at the position of 44, alanine at the position of 54 and tyrosine at the position of 138 is substituted with other amino acid(s) in the amino acid sequence of SEQ ID NO: 2. | 04-23-2015 |
| 20150140583 | METHODS, DEVICES, AND SYSTEMS OF DETECTING MICROORGANISMS - A rapid, sensitive method of separating and detecting microorganisms from a sample containing microorganisms, such as but not limited to bacteria, fungi, yeast, viruses, and the like. The method relies on separation techniques to separate and concentrate the cells from the sample, together with chemical techniques to amplify the amount of detectable signal from low numbers of cells to provide a rapid and sensitive method of detecting microorganisms. This detection method may utilize: a filtration device; a centrifugation device; a system; a swab device; and kit comprising one or more of the devices and components to perform the present method of separating and detecting microorganisms in a sample containing microorganisms. The sample may be a chemical, cosmetic, personal care, pharmaceutical, or consumable good in its raw material, in-process, and/or finished product states that needs to be tested for any contaminating microorganisms prior to shipment to the consumer. | 05-21-2015 |
| 20150299675 | MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF - A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2. | 10-22-2015 |
| 20150322481 | APPARATUS FOR DETECTING ATP IN A LIQUID SAMPLE - An apparatus ( | 11-12-2015 |
| 20150322482 | COMPOSITIONS AND METHODS FOR MONITORING TRANSMEMBRANE TRAFFICKING - Provided herein are compositions and methods for monitoring the movement of analytes and/or cellular components across biological membranes (e.g., cell surface internalization). In particular, reporter constructs are provided, the transmembrane movement of which (e.g., by endocytosis) is monitored by methods described herein. | 11-12-2015 |
| 20150337358 | APPARATUS AND METHODS FOR DETECTING ATP IN A LIQUID SAMPLE - Apparatuses and kits for the detection of ATP in a liquid sample are provided. The apparatuses and kits comprise a liquid reagent composition comprising luciferin and a sampling device having a sampling portion and a handling portion. The sampling portion comprises a fibrous or a foam material and is adapted to acquire and releasably retain a predetermined volume of a liquid sample or a sample of residue from a surface. The sampling device comprises a dry coating or a liquid coating, the coating including an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. Methods of use of the apparatus or kit are also provided. | 11-26-2015 |
| 20150337359 | LUCIFERASE-BASED ASSAYS - A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent. | 11-26-2015 |
| 20150376680 | PCL Compounds, Compositions, And Methods Of Use Thereof - Disclosed herein are compounds useful for detecting oxidants in a living cell, in a multicellular organism, or in a cell-free sample. In particular, disclosed herein are bioluminescent reporter compounds, and more particularly, fluorinated peroxy-caged-luciferin (PCL) compounds, compositions comprising such compounds, methods of using such compounds and compositions, and processes for preparing such compounds. Also disclosed herein are kits and methods for detecting and measuring peroxynitrite, and optionally, additional oxidants in a living cell, in a multicellular organism, or in a cell-free sample. | 12-31-2015 |
| 20160002703 | IMIDAZO[1,2-alpha]PYRAZINE DERIVATIVES - The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives. The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules. | 01-07-2016 |
| 20160010139 | DEVICES AND METHODS FOR TESTING THE CLEANLINESS OF MEDICAL INSTRUMENTS | 01-14-2016 |
| 20160010143 | METHOD FOR DETECTING PYROPHOSPHATE BY MEANS OF BIOLUMINESCENCE DETECTION | 01-14-2016 |
| 20160011173 | METHOD OF SCREENING A DRUG SUCH AS INSULIN SECRETAGOGUE | 01-14-2016 |
| 20160069864 | Reagents and Methods for Cancer Treatment and Prevention - The invention generally relates to the prevention and/or treatment of cancer, and, more specifically, to the treatment of tumors, including solid tumors and their metastases, without radiation or standard chemotherapeutic agents. In one embodiment, the invention involves a method comprising: a) providing a subject with tumor cells, b) removing at least a portion of said tumor cells from said subject to create removed cells, c) treating at least a portion of said removed cells ex vivo, using stimulating agents, including thapsigargin and/or thapsigargin-related compounds, so as to create treated tumor cells; and d) introducing said treated tumor cells (or fragments thereof) in vivo into the same subject to generate anticancer therapeutic effects. | 03-10-2016 |
| 20160076052 | DESIGN METHOD FOR SYNTHETIC GENES - The present invention provides a method of designing an optimized gene which comprises altering a nucleotide sequence of a target protein gene, so that only preferential codons with high frequency of use in human cells are selected and a GC content of not less than 60% is achieved. A gene design method which involves the feature “only preferential codons with high frequency of use are selected and a GC content of not less than 60% is achieved” can be established as a general rule for preparing proteins with high expression level, in order to obtain chemically synthesized genes for proteins capable of high-level expression in eukaryotes. | 03-17-2016 |
| 20160102338 | BIOLUMINESCENT SUCCINATE DETECTION ASSAY - Provided herein are methods for detecting and quantifying succinate in a sample. Also provided are methods for detecting and quantifying 2-oxoglutarate oxygenase enzyme and/or 2-oxoglutarate oxygenase activity in a sample and methods for screening for modulators of 2-oxoglutarate oxygenase activity. | 04-14-2016 |
| 20160169808 | LIQUID AND PLATE SENSORS FOR MICROPLATE INJECTOR SYSTEM | 06-16-2016 |
| 20160376332 | CYAN-EXCITABLE ORANGE-RED FLUORESCENT PROTEINS AND BIOLUMINESCENT RESONANCE ENERGY TRANSFER SYSTEMS - Engineered orange-red fluorescent proteins with enhanced fluorescent properties, obtained by mutagenesis of mNeptune2, are disclosed. In particular, the invention relates to engineered orange-red fluorescent proteins excitable with cyan light having increased emission intensity and their use in bioluminescent resonance energy transfer systems and fluorescence and bioluminescence imaging. | 12-29-2016 |
| 20160376568 | THIENOPYRROLE COMPOUNDS AND USES THEREOF - Thienopyrrole compounds that may inhibit | 12-29-2016 |
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