| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 435700720 | Assay in which a label present is an enzyme substrate or substrate analogue | 78 |
| 20080227124 | Solid phases optimized for chemiluminescent detection - Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer. | 09-18-2008 |
| 20080293081 | Fluorescence Polarization Assays for Acetyltransferase/Deacetylase Activity - Provided are methods for determining the activity of proteins that modulate the acetylation state of a protein substrate. The methods may be used for determining both acetyltransferase activity and deacetylase activity. The methods involve fluorescence polarization measurements for determining the acetylation state of a substrate peptide. The methods may also be used to identify compounds that modulate the activity of a protein having acetyltransferase or deacetylase activity. Also provided are substrates for acetyltransferase or deacetylase enzymes for use in association with a fluorescence polarization assay. | 11-27-2008 |
| 20080305504 | Compositions, methods, and kits for assaying complement activation - The present invention provides a fluorogenic composition for assaying complement activation that comprises a substrate for C3 convertase that is linked to a first fluorophore and a second fluorophore, wherein the fluorescence of the first and second fluorophores are mutually substantially quenched when the two fluorophores are present at a distance less than the characteristic distance for the two fluorophores. In one embodiment, the fluorescence of the first fluorophore is substantially quenched by the second fluorophore, and the second fluorophore emits heat upon quenching. The present invention also provides a method for assaying complement activation, wherein the method includes the steps of incubating a biological sample with a polymer to provide a polymeric biological sample, followed by incubating the polymeric biological sample with the fluorogenic composition, and measuring the fluorescence. The method may optionally include the step of measuring the optical properties of the biological sample prior to the incubation steps. The present invention further provides a kit for assaying complement activation that comprises the fluorogenic composition disclosed herein. | 12-11-2008 |
| 20080311598 | Magnetic Biosensor For Determination of Enzymic Activity - Magnetic sensors are very suitable for use in determination of enzymatic activity. In a preferred embodiment the invention relates to a method for determining activity of an enzyme in modification of substrate ( | 12-18-2008 |
| 20080318255 | Fluorescent Labeled Sphingosines - The present invention relates to a method (assay) for determining the activity of an enzyme selected from the group consisting of a sphingosine kinase and a phosphatase involved in the sphingolipid pathway by use of a labeled sphingosine. | 12-25-2008 |
| 20080318256 | Method for Stabilizing Coenzyme and Composition Therefor - Disclosed is a sugar and/or a sugar alcohol as a substance for suppressing dephosphorylation reaction of a phosphorylated coenzyme. Also disclosed is a method for stabilizing a phosphorylated coenzyme which is characterized by having at least a substance for suppressing dephosphorylation reaction of the phosphorylated coenzyme coexist with the phosphorylated coenzyme. | 12-25-2008 |
| 20090004677 | QUANTITATIVE DETERMINATION OF BCL10 - Methods of and kits for quantitatively determining the concentrations of Bcl10 in a biological sample were developed that provide an accurate means of identifying therapeutic molecules with a plurality of therapeutic effects. A solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) for human Bcl10 that provides reproducible, precise measurements was developed and characterized. The sensitivity of the assay is 0.25 ng/ml, enabling accurate detection of small quantities of Bcl10. The sensitive and specific, solid-phase, sandwich ELISA for Bcl10 is well-suited for the accurate determination of Bcl10 values in different experimental conditions, in immune and non-immune cells, and may find use in a clinical context. An ELISA as described herein may have particular clinical utility, since increased Bcl10 is associated with inflammation, infection and malignancy. | 01-01-2009 |
| 20090017477 | METHOD FOR DETERMINATION OF CONCENTRATION, CHARGE OR UNIT SIZE OF A SUBSTANCE - This invention relates to a method comprising the steps of a) contacting a sample containing a sample substance and a solid phase comprising a signal element, and optionally a substance containing a signal element, i.e. a signal element substance; wherein at least one of said sample substance, said solid phase, and said signal element substance comprises a signal element; wherein the surface of the solid phase contains no specific binding partners; and wherein the solid phase is capable of binding said sample substance nonspecifically, preferably through adsorption, to said solid phase, and b) detecting a signal change resulted from binding of said sample substance and/or signal element substance to said solid phase, and/or change in distance from said sample substance and/or said signal element substance to said solid phase. | 01-15-2009 |
| 20090023163 | Method of Measuring Enzyme Activity and Method of Evaluating Compound - It is intended to provide a method of measuring an activity, which is a method of measuring an activity of an enzymatic reaction in which a lipid-soluble reaction product is formed by catalyzing an addition reaction of two or more kinds of substrates, characterized by comprising a labeling step in which any one of the substrates is labeled, a reaction step in which an enzymatic reaction is carried out in the presence of an enzyme, all substrates and an acceptor, and a detection step in which the reaction product is detected by bringing the labeled molecule and a molecule to be detected close to each other via the acceptor and transferring an energy generated by the labeled molecule to the molecule to be detected, and a method of evaluating a compound utilizing the method of measuring an activity. By the methods of the invention, it is possible to perform a treatment from the enzymatic reaction to the measurement of an enzyme activity with the simple steps and it is possible to provide a method of measuring an enzyme activity which can be applied to an HTS evaluation system. | 01-22-2009 |
| 20090047692 | OPTICALLY-DETECTABLE ENZYME SUBSTRATES AND THEIR METHOD OF USE - The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay. | 02-19-2009 |
| 20090053741 | ASSAY FOR MEASURING ASYMMETRIC METHYLARGININE IN A BIOLOGICAL SAMPLE - The invention relates to a method of determining the presence and/or amount of an asymmetric methylarginine in a sample, the method comprising:
| 02-26-2009 |
| 20090098582 | METHOD FOR DETERMINING RISK OF CANCER RELAPSE AND COMPUTER PROGRAM PRODUCT - The invention provides a method for determining a risk of cancer relapse comprising a step of determining the risk of cancer relapse on the basis of a comprehensive activity value of a transmembrane tyrosine kinase of a tumor cell, as well as a computer program product for determining a risk of a cancer relapse. | 04-16-2009 |
| 20090298100 | Monoclonal Antibody Against D-Dimer and Diagnosis Agent for Detecting D-Dimer, Crosslinked Fibrin and its Derivatives Containing D-Dimer by Using the Antibody - Disclosed are a monoclonal antibody against human D-dimer produced in a mouse and high molecular weight crosslinked fibrin including a corresponding epitope, a cell line secreting the monoclonal antibody, and a method for manufacturing the same. The anti-D-dimer monoclonal antibody of the present invention may be effectively used as a diagnosis agent for screening and detecting in vivo D-dimer, and high molecular weight crosslinked fibrin and its derivatives containing the D-dimer since the monoclonal antibody specifically reacts with D-dimer, and crosslinked fibrin and its derivatives containing the D-dimer, which do not bind to human fibrinogen or fibrin. | 12-03-2009 |
| 20090311723 | FLUORESCENCE-BASED ASSAY FOR MONOACYLGLYCEROL LIPASE COMPATIBLE WITH INHIBITOR SCREENING - The present invention provides reagents, kits and methods for assaying monoglycerol lipase activity and for identifying compounds that modulate monoglycerol lipase (“MGL”) activity. A simple, sensitive fluorescence assay, which is amenable to high throughput screening, is described. In one embodiment, 7-Hydroxycoumarinyl-arachidonate (7-HCA) is used as a fluorogenic substrate for MGL, which catalyzes the hydrolysis of 7-HCA to generate arachidonic acid and the highly fluorescent 7-hydroxycoumarin (7-HC). Release of 7-HC is monitored continuously using a fluorometer. MGL protein catalyzed the hydrolysis of 7-HCA with an apparent KM of 9.8 mM and Vmax of 1.7 mmoles min | 12-17-2009 |
| 20090325203 | Assay For Tissue Factor And Factor Vlla - The invention provides assays for detecting and quantitating tissue factor and factor VIIa in simple and complex biological systems. The assays are performed by detecting and/or measuring the tissue factor cofactor activity and factor VIIa enzymatic activity using aminonaphthalene-based fluorogenic substrates. | 12-31-2009 |
| 20100028916 | Method for the detection of enzymatic reactions - The present invention provides a method for the detection of an enzyme E1 in a liquid sample comprising the steps of: a) providing a complex (Sa-Sb-M), wherein (Sa-Sb) is a substrate S of E1 cleavable into Sa and Sb by E1, and M is a marker linked to Sb, b) incubating the sample with the complex under conditions enabling the cleavage of S into Sa and Sb by E1, c) separating non-cleaved complex (Sa-Sb-M) from the sample, and d) measuring M in the sample. Furthermore, the present invention further provides kits and devices for the detection of an enzyme E1. | 02-04-2010 |
| 20100047828 | SOLID-PHASE DETECTION OF TERMINAL MONOSACCHARIDES CLEAVED FROM GLYCOSYLATED SUBSTRATES - The present invention relates to a novel method for analysing carbohydrates. The invention is in particular useful in detecting a terminal monosaccharide which may be released from a glycosylated substrate for example using an exoglucosidase. After relase from the glycosylated substrate the terminal monosaccharide may be captured on a solid support, incubated with a boronate detection agent and detected by aid of the boronate detection agent. The methods of the invention are useful for a variety of purposes including sequencing of carbohydrates, wherein exoglycosidases with predetermined specificity are employed for the release. | 02-25-2010 |
| 20100086952 | ASSAYS FOR MEASURING PHOSPHATE MODIFICATION ENZYME ACTIVITY - The present invention relates to assays that can measure the activity of enzymes that catalyze phosphate modifications, such as kinases, phosphatases, cyclases and phosphodiesterases. The assays can also be used to identify and screen for substances that modulate the activity of kinases, phosphatases, cyclases and phosphodiesterases. | 04-08-2010 |
| 20100105083 | Methods For Determining The Levels Of TGF-Beta In A Composition - A novel method for determining the levels of TGF-β1 or TGF-β2 in a sample of milk, raw protein source, or nutritional composition is provided. The method involves, in some cases, reconstituting the sample; in some cases, centrifuging the sample; activating the sample using particular ratios of sample:acid:base; diluting the sample using particular ratios of sample:buffer agent; and determining the concentration of TGF-β1 in the sample using an ELISA assay. | 04-29-2010 |
| 20100112604 | HOME FOOD TEST KIT AND METHOD OF USE - A home test kit for detecting the presence or absence of melamine (1,3,5-triazine-2,4,6-triamine) has a sample extract container in which a volume of extract solution is present; a test strip, the test strip being a lateral flow device for detecting melamine (1,3,5-triazine-2,4,6-triamine) in a food or beverage sample; and wherein a food or beverage sample is placed in the sample extract container to form an aqueous sample extract solution and the test strip is inserted directly into the aqueous sample for an instructed time then the strip is removed and observed for a color change. The method of using the kit involves a visual observation without requiring any other equipment. | 05-06-2010 |
| 20100233735 | PHARMACEUTICALS AND METHODS FOR TREATING HYPOXIA AND SCREENING METHODS THEREFOR - Light-generating fusion proteins having a ligand binding site and a light-generating polypeptide moiety and their use as diagnostics, in drug screening and discovery, and as therapeutics, are disclosed. The light-generating fusion protein has a feature where the bioluminescence of the polypeptide moiety changes upon binding of a ligand at the ligand binding site. The ligand may be, for example, an enzyme present in an environment only under certain conditions, e.g., ubiquitin ligase in a hypoxic state, such that the light-generating fusion protein is “turned on” only under such conditions. | 09-16-2010 |
| 20100248268 | Methods to utilize invertebrate chemosensory proteins for industrial and commercial uses - This invention provides methods and compositions for using chemosensory proteins derived from invertebrates to bind or detect specific compounds. Applications include use as detector elements in biosensors or other sensory devices and purification or concentration devices. Examples of proteins involved in the chemosensory pathway include odorant binding proteins (OBPs), sensory appendage proteins (SAPs), orthologs of the | 09-30-2010 |
| 20100304407 | Fluorescence Lifetime and Fluorescence Assays - The invention provides a method for determining a degree of phosphorylation of a substrate, for example a peptide substrate, using a fluorescence probe that acts alone or with another material and has a lifetime that varies when in proximity to a phosphate, the method comprising: causing the fluorescence probe to fluoresce; measuring a time response of the fluorescence, and analysing the fluorescence time response to identify a fluorescence component having a lifetime associated with phosphorylated substrate and a fluorescence component having a lifetime associated with un-phosphorylated substrate. | 12-02-2010 |
| 20100323372 | IMMUNOASSAY ASSEMBLY AND METHODS OF USE - The present invention relates to an improved system for efficiently and accurately performing immunoassays, such as ELISAs. The invention provides an immunoassay assembly which includes a flow-through unit and an aspiration pump. The immunoassay flow-through unit includes an outer seal; at least one bed support; an inner seal; and a packed non-porous bed. The unit is releasably attached to an aspiration pump which enables the controlled flow rate of liquid passing through the packed bed of the flow-through unit. The invention also provides a method of using the immunoassay assembly to identify analytical targets of interest. | 12-23-2010 |
| 20110086370 | Protease Detection - A polypeptide comprising a chromogenic amino acid. The chromogenic amino acid is flanked by at least one amino acid to the N and C termini thereof. The amine group of the chromogenic amino acid has a pKa of less than 5. The chromogenic amino acid is capable of reacting with a conjugated aldehyde. The polypeptide comprises a target sequence for a target protease which is capable of cleaving the peptide bond comprising the amino group of the chromogenic amino acid. | 04-14-2011 |
| 20110091909 | Stabilizing Agents and Capture Ligands for Use in Assays Measuring Analyte Concentrations - The present invention is related to compositions useful for the measurement of free or unbound analyte concentrations in a fluid. The present invention includes the use of capture ligands and stabilizing agents to improve the accuracy of analyte concentration assays. Methods and tools for using the present invention are also disclosed. | 04-21-2011 |
| 20110159523 | N-TERMINAL SPECIFIC CHEMICAL LABELING FOR PROTEOMICS APPLICATIONS - Described herein is a method that may be used in various applications, such as drug development, medical diognosis and gene/protein therapy. In one embodiment, the subject matter discloses an effective method for identification and quantification of large sets of proteins/peptides in vitro and cells in vivo. | 06-30-2011 |
| 20110159524 | METHOD FOR MONITORING HYDROLYTIC ACTIVITY - The present invention relates to methods of measuring the activity of a hydrolytic agent comprising contacting a biomolecule with a hydrolytic agent in the presence of a fluorescent dye under conditions that allow digestion of the biomolecule by the hydrolytic agent. The fluorescence of the dye is monitored over time and a change in fluorescence signifies digestion of the biomolecule by the hydrolytic agent. The biomolecule is preferably a protein, peptide or proteome but can also be a carbohydrate, oligonucleotide or lipid. Further methods relate to determining an end point for digestion of a biomolecule by a hydrolytic agent, and methods of monitoring digestion of a biomolecule by a hydrolytic agent. The monitoring can be performed on the reaction mixture in real time or via sampling. The invention also relates to kits for carrying out the method. | 06-30-2011 |
| 20110159525 | METHODS TO DETERMINE IMMUNOGENICITY OF HUMANIZED ANTI-TAG 72 CC49 ANTIBODIES - The present disclosure provides humanized CC49 monoclonal antibodies that bind TAG-72 with high binding affinity and that are minimally immunogenic. In one embodiment, a humanized CC49 antibody includes a non-conservative amino acid substitution in a light chain complementarity determining region 3 of the CC49 antibody. In a further embodiment, the humanized CC49 antibody includes a non-conservative substitution of a first residue in a light chain complementarity determining region 3 and a substitution of a second residue in a complementarity determining region of the humanized CC49 antibody. In several of the embodiments, methods are disclosed for the use of a humanized CC49 antibody. | 06-30-2011 |
| 20110183361 | Nonseparation Assay Methods - Assay methods are disclosed involving specific binding reactions which are simplified compared to known methods. A compound capable of producing chemiluminescence is immobilized on a solid support as is a member of a specific binding pair for capturing an analyte from a sample. An activator compound that activates the chemiluminescent compound and is conjugated to a specific binding pair member is added in excess along with the sample to the solid support. Addition of a trigger solution causes a chemiluminescent reaction at the sites where the activator conjugate has been specifically bound. The assay methods are termed non-separation assays because they do not require removal or separation of excess detection label (activator conjugate) prior to the detection step. The methods are applicable to various types of assays including immunoassays, receptor-ligand assays and nucleic acid hybridization assays. | 07-28-2011 |
| 20110250621 | METHOD FOR IN VITRO ASSAY OF THE CIRCULATING TISSUE FACTOR, AND USE IN THE DETECTION OF COAGULATION DISEASES - The invention concerns the field of hemostasis, in particular blood coagulation disorders linked to an abnormal expression of tissue factor, and to physiopathological phenomena correlated with over-expression of the factor. The present invention provides a method for assaying the activity of circulating tissue factor in a biological sample. The method of the invention is carried out in vitro, in particular on a blood sample collected from a patient. | 10-13-2011 |
| 20110262938 | Determination of the amount of hLHB core fragment in a sample from a subject and uses thereof - The present invention provides a method for predicting the likely timing of the onset of menopause for a perimenopausal female subject by determining the amount of hLHβcf in a sample from the subject comprising the steps of: (a) contacting a sample from the subject with an antibody which specifically binds to hLHβcf without substantially cross-reacting with hLH, hLHβ or hLHβcf, under conditions permitting formation of a complex between the antibody and hLHβcf; (b) measuring the amount of complex formed, so as to thereby determine the amount of hLHβcf in the sample; and (c) comprising the amount of hLHβcf in the subject's sample determined in step (b) with either (i) the amount determined for known postmenopausal female subject or (ii) the amount determined for a sample from a known premenopausal female subject, wherein an amount of hLHβcf in the sample similar to the amount of hLHβcf in the known postmenopausal sample indicates temporal proximity to the onset of menopause, and an amount of hLHβcf in the sample similar to the amount of hLHβcf in the known premenopausal sample indicates temporal distance from the onset of menopause for the subject. AS described herein amount is both concentration and pattern of measurement of concentrations in one or more consecutive urine specimens. | 10-27-2011 |
| 20110269156 | Nonseparation Assay Methods - Assay methods are disclosed involving specific binding reactions which are simplified compared to known methods. A compound capable of producing chemiluminescence is immobilized on a solid support as is a member of a specific binding pair for capturing an analyte from a sample. An activator compound that activates the chemiluminescent compound and is conjugated to a specific binding pair member is added in excess along with the sample to the solid support. Addition of a trigger solution causes a chemiluminescent reaction at the sites where the activator conjugate has been specifically bound. The assay methods are termed non-separation assays because they do not require removal or separation of excess detection label (activator conjugate) prior to the detection step. The methods are applicable to various types of assays including immunoassays, receptor-ligand assays and nucleic acid hybridization assays. | 11-03-2011 |
| 20110306066 | HOMOGENEOUS ACTIVITY TEST FOR DETERMINING ENZYMATIC REACTIONS - A homogeneous method for determining the enzymatic activity of an analyte in a sample permits simultaneous determination of the amount of enzyme in the same reaction mixture and determination of enzyme activators or inhibitors. | 12-15-2011 |
| 20120064546 | METHODS FOR DETECTION OF HYDROPHOBIC DRUGS - Methods and reagents are disclosed for pretreating a sample suspected of containing a hydrophobic drug for conducting an assay method for detecting the hydrophobic drug. A combination is provided in a medium that includes the sample, a releasing agent for releasing the hydrophobic drug and the metabolites from endogenous binding moieties, and a selective solubility agent that provides for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The selective solubility agent includes a water miscible, non-volatile organic solvent and is present in the medium in a concentration sufficient to provide for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The medium, which may further include a hemolytic agent, is incubated under conditions for releasing the hydrophobic drug and the metabolites from endogenous binding moieties. The pretreated sample may be subjected to an assay for determining the hydrophobic drug. | 03-15-2012 |
| 20120070852 | ASSAY - The present invention relates to an assay for transferases. The assay comprises a first moiety comprising a transferase substrate and a second moiety capable of binding to the transferase substrate after it has been acted on by the transferase. One of the first and second moieties comprises a fluorophore and the other of the first and second moieties causes a change in fluorescence of the fluorophore. Thus, when the second moiety binds the transferase substrate after it has been acted on by the transferase, a change in fluorescence can be detected. The assay allows for agents that modulate the activity of the transferase to be screened. | 03-22-2012 |
| 20120094313 | Nonseparation Assay Methods - Assay methods are disclosed involving specific binding reactions which are simplified compared to known methods. A compound capable of producing chemiluminescence is immobilized on a solid support as is a member of a specific binding pair for capturing an analyte from a sample. An activator compound that activates the chemiluminescent compound and is conjugated to a specific binding pair member is added in excess along with the sample to the solid support. Addition of a trigger solution causes a chemiluminescent reaction at the sites where the activator conjugate has been specifically bound. The assay methods are termed non-separation assays because they do not require removal or separation of excess detection label (activator conjugate) prior to the detection step. The methods are applicable to various types of assays including immunoassays, receptor-ligand assays and nucleic acid hybridization assays. | 04-19-2012 |
| 20120237955 | METHODS FOR IDENTIFYING INHIBITORS OF BOTULINUM NEUROTOXINS - A system and method for identifying a | 09-20-2012 |
| 20120270240 | Detection Reagents for Tyrosine Kinase Activity and Methods of Use Thereof - Dual colored fluorescent indicators of specific tyrosine kinase activity and methods of use thereof are disclosed. | 10-25-2012 |
| 20120270241 | SYSTEMS AND METHODS FOR EVOLVING ENZYMES WITH DESIRED ACTIVITIES - The present invention provides a new method for engineering or evolving enzymes to have desirable characteristics. Among the desirable characteristics is the ability to control catalytic activity through the use of a trigger molecule that rescues a catalytic site defect introduced during the engineering process. The method includes co-evolving enzyme and substrate to retain or improve substrate binding activity in the absence of catalytic activity. | 10-25-2012 |
| 20120270242 | IMMUNOCHEMICAL DETECTION OF SINGLE TARGET ENTITIES - The present invention relates to immunochemical visualization and quantification of single target entities, such as single molecules, single molecular structures, single particles, etc. in samples wherein said single entities are immobilized. In particular, the invention relates to methods for visualization and quantification of single units of biological or chemical targets, in particular to immunochemical visualization of single molecules of biological targets in histological samples. The methods of the invention comprise a step of forming discrete deposits of detectable molecules at single target sites of sample mediated by an enzyme with oxydoreductase activity, wherein a single target site comprises a single unit of a target. The invention also relates to assays comprising the present visualization and quantification methods and diagnostic applications of said methods. | 10-25-2012 |
| 20130102012 | FLUORESCENT-LABELLED DIUBIQUITIN SUBSTRATE FOR A DEUBIQUITINASE ASSAY - The application relates to a substrate for measuring the activity of a deubiquitinating enzyme (DUB), comprising a diubiquitin molecule, wherein an ubiquitin monomer is labeled with a fluorescent label, as well as an assay for DUB enzymes using such substrates. | 04-25-2013 |
| 20130130282 | Fibrinogen Assay - The present invention is directed to a method of detecting intact fibrinogen, comprising the steps of: a) providing a sample containing at least some fibrinogen optionally converted at least in part to fibrin, and optionally containing thrombin; b) solubilizing the sample in a solubilizing solution that inhibits thrombin activity; c) after optional SDS-PAGE transferring/applying a portion of said sample to a protein-binding membrane; d) reacting the fibrinogen with a primary monoclonal antibody capable of binding to fibrinopeptide A moiety; and e) detecting the quantity of intact fibrinogen in the sample by quantifying the amount of the bound primary monoclonal antibody. | 05-23-2013 |
| 20130196352 | BACTERICIDAL ANTIBODY ASSAYS TO ASSESS IMMUNOGENICITY AND POTENCY OF MENINGOCOCCAL CAPSULAR SACCHARIDE VACCINES - The disclosure provides compositions, methods and kits for assessing immunogenicity, potency, or both, of meningococcal capsular saccharide vaccines. The assessment is based upon measurement of binding of a bactericidal antibody to a capsular saccharide component in the vaccine. | 08-01-2013 |
| 20130217039 | OPTICAL PATHOGEN DETECTION SYSTEM AND QUALITY CONTROL MATERIALS FOR USE IN SAME - A system for detecting presence of an organism having an enzyme in a sample, comprising: a cartridge for containing the sample and a substrate such that the enzyme can react with the substrate to produce a biological molecule; a partitioning element mounted in a recess in a base of the cartridge, the partitioning element allowing partitioning of the biological molecule thereinto; a light source for irradiating the biological molecule partitioned into the partitioning element; and, a detector for detecting fluorescence of the biological molecule partitioned into the partitioning element, the detected fluorescence being indicative of presence of the organism in the sample; wherein the light source is in a raised cartridge mount of the system that mates with the recess in the base of the cartridge. Also provided is a method for calibrating said system and quality control cartridges for use in same. | 08-22-2013 |
| 20130217040 | METHOD AND SYSTEM FOR DETECTING BIOLOGICAL MOLECULES IN SAMPLES - A system for detecting presence of an organism having an enzyme in a sample, comprising: a cartridge for containing the sample and a substrate such that the enzyme can react with the substrate to produce a biological molecule; a partitioning element mounted in a recess in a base of the cartridge, the partitioning element allowing partitioning of the biological molecule thereinto; a light source for irradiating the biological molecule partitioned into the partitioning element; and, a detector for detecting fluorescence of the biological molecule partitioned into the partitioning element, the detected fluorescence being indicative of presence of the organism in the sample; wherein the light source is in a raised cartridge mount of the system that mates with the recess in the base of the cartridge. | 08-22-2013 |
| 20130217041 | DETECTION OF BIOLOGICAL MOLECULES BY DIFFERENTIAL PARTITIONING OF ENZYME SUBSTRATES AND PRODUCTS - This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples. | 08-22-2013 |
| 20130217042 | TEST STRIP CARD - The present invention relates to a test strip for determining and/or quantifying a property of a sample, such as the concentration of an analyte, the pH, the viscosity, or the specific gravity of a fluid specimen. More particularly, the present invention relates to an improved test strip and scale for determining and/or quantifying a property of a sample, such as the concentration of an analyte, the pH, the viscosity, or the specific gravity of a fluid specimen. | 08-22-2013 |
| 20130224767 | IMMUNOCHROMATOGRAPHIC ASSAY METHOD AND APPARATUS - In an immunochromatographic assay method for performing an assay related to a specimen by reacting a reagent held by a carrier and the specimen with each other, and by obtaining an image signal by imaging a reagent portion by a camera, and by measuring, based on the image signal, the color-developed state of the reagent, a morphological opening-operation is applied to the image signal by using a structuring element the size of which is less than or equal to a signal-change-width of the reagent portion that has developed color, and a morphological opening-operation is applied to the image signal by using a structuring element the size of which is larger than the signal-change-width of the reagent portion that has developed color. The color-developed state is measured based on a difference signal obtained by performing a subtraction between the image signals to which the opening-operations have been applied respectively. | 08-29-2013 |
| 20130224768 | IMMUNOCHROMATOGRAPHIC ASSAY METHOD AND APPARATUS - In an immunochromatographic assay method for assaying a specimen by reacting a reagent held by a carrier and the specimen with each other, and by obtaining an image signal by imaging a reagent portion that has been reacted, and by measuring, based on the image signal, the color-developed state of the reagent caused by the reaction, the carrier holds reagents in such a manner that they do not extend continuously for the full length of the carrier in any one of two-dimensional directions along the surface of the carrier. A morphological opening operation is applied to the image signal, with respect to each of the two-dimensional directions, by using a structuring element the size of which is less than or equal to a signal change width of the reagent portion that has developed color, and the color-developed state is measured based on the image signal after the opening operation. | 08-29-2013 |
| 20130244257 | INTEGRATED MICROFLUIDIC RADIOASSAY AND IMAGING PLATFORM FOR SMALL SAMPLE ANALYSIS - An immunocapture-based in vitro kinase assay on an integrated polydimethylsiloxane (PDMS) microfluidics platform that can reproducibly measure kinase activity from as few as 3,000 cells is described. For this platform, the standard radiometric | 09-19-2013 |
| 20130252261 | COMPOSITIONS AND METHODS FOR IN VITRO DIAGNOSTIC TESTS INCLUDING SULFONIC ACID COMPOUND - The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the chromogenic reaction product can be maintained over significantly longer periods of time of that of conventional reagents and methods. The sulfonic acid can be used in assays such as ELISAs in order to provide a more accurate and safer detection of analytes in a biological sample. | 09-26-2013 |
| 20130252262 | DROPLET-BASED AFFINITY ASSAYS - A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample. | 09-26-2013 |
| 20130323758 | MEASUREMENT DEVICE AND MEASUREMENT METHOD - A measurement device that includes a plurality of lines for conveying a reaction container containing a sample and measures a predetermined material included in the sample while conveying the reaction container by the plurality of lines, wherein the plurality of lines include: a first reaction line for conveying a reaction container at a first convey speed; a second reaction line for conveying a reaction container at a second convey speed; and a measurement line for measuring a predetermined material included in a sample reacted with a reagent within the reaction container in the first reaction line and a sample reacted with a reagent within the reaction container in the second reaction line, the measurement line conveying the reaction containers at a third convey speed that is higher than the first convey speed and the second convey speed. | 12-05-2013 |
| 20130330743 | METHODS, KITS AND SYSTEMS FOR SIGNAL AMPLIFICATION FOR BIOASSAYS USING ZINC NANOPARTICLES - Disclosed herein are methods, kits and systems for signal amplification that can be used for many analytes. For example, a method of detecting an analyte in a sample includes contacting the sample containing the analyte with a detecting agent, wherein the detecting agent contains a specific binding agent that binds the analyte and zinc nanoparticles wherein the zinc nanoparticles and specific binding agent are coupled together; exposing the analyte bound to the specific binding agent which is coupled to the zinc nanoparticles to an acidic condition to release zinc ions from the zinc nanoparticles; contacting the released zinc ions with an indicator to generate a signal; and detecting the signal. The disclosed bioassay can be used in clinical and non-clinical settings. For example, the method can be used for clinical diagnosis, prognosis, and/or treatment-effectiveness or for testing for the presence of a substance like biological or chemical agents. | 12-12-2013 |
| 20140030738 | SOLUBLE HUMAN M-CSF RECEPTOR AND USES THEREOF - Soluble human M-CSF receptor is provided, along with pharmaceutical compositions containing such receptor, kits containing a pharmaceutical composition, and methods of diagnosing and treating diseases and disorders associated with M-CSF such as bone loss in a subject afflicted with an osteolytic disease. | 01-30-2014 |
| 20140057290 | METHOD FOR PERFORMING A BIOCHEMICAL ANALYSIS, ESPECIALLY IN OUTER SPACE - The invention is based on a method for performing a biochemical analysis, especially in outer space, wherein at least one analyte in a sample is determined qualitatively and/or quantitatively by means of selective binding of an analyte-specific pair composed of a binding substance and a detection substance to the analyte and by labeling by a labeling substance, and wherein the sample, the binding substance, the detection substance and the labeling substance are mixed in a reaction vessel in one method step. It is proposed that the mixing be brought about by means of mixing bodies. | 02-27-2014 |
| 20140057291 | BISUBSTRATE FLUORESCENT PROBES FOR PROTEIN KINASE CK2 - This invention relates to fluorescent probes for screening and characterization of compounds binding to protein kinase CK2, measurement of concentration of the catalytically active form of CK2 and imaging of CK2 activity in cells and tissues. The CK2-selective probe of the present invention interacts with binding sites of both substrates of the catalytic subunit of CK2 and therefore can be used for characterization of all inhibitors binding to the active site of CK2 in the binding/displacement assay. The high affinity of the probe affords the detection of the enzyme at low concentration and characterization of inhibitors in a wide affinity range. The invention also relates to the application of the probes for mapping and monitoring of CK2 activity in cells, tissues and live organisms. | 02-27-2014 |
| 20140065645 | POLYPEPTIDE LINKER AND METHOD OF ANALYZING TARGET MATERIAL USING THE SAME - A polypeptide linker comprising an antibody-binding region and an enzyme cleavage region, and related compositions, kits, and methods of using same. | 03-06-2014 |
| 20140093891 | DEVICE FOR PERFORMING A BIOCHEMICAL ANALYSIS, ESPECIALLY IN OUTER SPACE - A device for performing a biochemical analysis, especially in outer space, more particularly an immunoassay, in which analysis at least one analyte in a sample is determined selectively, having at least one reaction container which has at least one work volume which is intended for taking in a liquid volume and for performing at least one substep of an analysis reaction, and having at least one interface which is intended for connecting at least one work volume to a further media container. | 04-03-2014 |
| 20140134649 | METHOD AND KIT FOR DETECTING THE PREOVULATORY LH PEAK - The present invention relates to a method of detecting the preovulatory LH peak in a biological sample obtained from mammals and a kit for applying the method of detection. | 05-15-2014 |
| 20140206020 | Voriconazole Immunoassays - Methods, compositions and kits are disclosed directed at voriconazole derivatives, immunogens, signal generating moieties, antibodies that bind voriconazole and immunoassays for detection of voriconazole. | 07-24-2014 |
| 20140234870 | NIK Inhibitors cell-based screening assay - The present invention relates to a cell-based assay for the screening and the identification of inhibitors of NIK protein. The method according to the present invention allows the identification of compounds that inhibit NIK activity related to the phosphorylation of IKK1. The assay combines the use of transfected Human Embryonic Kidney (HEK) cells with a non-radioactive, homogeneous proximity assay using an “acceptor” bead and a “donor” bead. | 08-21-2014 |
| 20140273021 | VITAMIN D ASSAYS - The disclosure relates to methods for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid sample. The disclosure further relates to kits for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid. | 09-18-2014 |
| 20140287439 | METHOD FOR MANUFACTURING MULTIPLE-DIAGNOSIS MEMBRANE SENSOR BY USING SCREEN PRINTING - Provided is a method for manufacturing a multiple-diagnosis membrane sensor provided with multiple channels by using screen printing, and more specifically, to a method for manufacturing a membrane sensor capable of performing multiple-diagnosis by screen-printing hydrophobic ink on a membrane to form multiple channels. | 09-25-2014 |
| 20140302531 | ENZYMATIC ACTIVITY BASED-DETECTION - Disclosed herein are methods and kits which are useful for detecting presence of an enzyme in a test sample based upon the intrinsic enzymatic activity of such test sample. The present invention provides the ability to evaluate cell culture conditions and optimize the desired glycoform content of recombinantly prepared enzymes. | 10-09-2014 |
| 20140356885 | Reducing Non-Specifically Bound Molecules on Supports - Methods and reagents are disclosed for preparing a support for reaction of the support with an assay molecule. In the method the support is treated with a detergent at a concentration of about 0.01% to about 5% (by weight) at a temperature of about 4° C. to about 50° C. for a period of about 1 hour to about 24 hours and subsequently the support is washed. The support is contacted with the assay molecule under conditions for covalently binding the assay molecule to the support to form a conjugate of the support and the assay molecule. | 12-04-2014 |
| 20140377783 | NOVEL APPLICATIONS OF INDOLOINDOLE AND INDOLOQUINOLINE DYES - A novel type of dye systems comprises a selection of 10H-indolo[1,2-a]indole compounds (henceforth abbreviated as IO compounds) and (5H,7H)-indolo[1,2-a]quinoline compounds (henceforth abbreviated as IQ compounds) showing a solvatochromic effect and exhibiting strong fluorescence in a variety of materials such as polypropylene, polyethylene, oils, various solvents, emulsions. Also disclosed are various methods how the IO/IQ compounds can be administered, especially how they can be produced and administered in situ from a precursor, responding to external stimuli such as enzyme activity, temperature and so forth. The response of a precursor to external stimuli can also be used to determine the presence or absence of such stimuli. | 12-25-2014 |
| 20150024415 | DETECTION AND QUANTIFICATION OF ANALYTES BASED ON SIGNAL INDUCED BY ALKALINE PHOSPHATE - A general methodology for highly sensitive and selective sensors that can achieve portable, low-cost and quantitative detection of a broad range of targets using only a personal glucose meter (PGM) is disclosed. The method and sensors take advantage of the ability of alkaline phosphatase (ALP) to convert glucose-1-phosphate to glucose, and the ability of PGMs to detect the generated glucose. The disclosed sensors can be part of a lateral flow device. Methods of using such sensors for detecting target agents, for example to diagnose disease, are also provided. | 01-22-2015 |
| 20150132778 | METHODS AND KITS FOR DIAGNOSING HEPARIN-INDUCED THROMBOCYTOPENIA - Provided herein are methods for diagnosing heparin-induced thrombocytopenia (HIT) in a subject. The methods comprise (a) contacting a PF4-heparin complex with a biological sample from the subject; (b) contacting the composition of part (a) with an HIT-like antibody; and (c) measuring the amount of bound HIT-like antibody. In one embodiment, a significant decrease in the amount of bound HIT-like antibody in the composition of part (b) as compared to a control level is indicative of a diagnosis of HIT in the subject. Also provided are assay kits for performing the methods of the invention. | 05-14-2015 |
| 20150147764 | POLYPEPTIDE SUBSTRATE FOR THE DETECTION OF VON WILLIEBRAND FACTOR CLEAVING PROTEASE ADAMTS13 - In a first aspect, there is provided an isolated polypeptide substrate for a disintegrin-like and metallopeptidase with thrombospondin type-1 motif, 13 (ADAMTS13) that is from 45 to 70 amino acids in length and has an amino acid sequence that is substantially similar to part of the von Willebrand factor A2 domain sequence set forth in SEQ ID NO:2, with one or more of the following modifications: (i) the amino acid corresponding to position 1599 of SEQ ID NO: 2 is mutated from Q to K; (ii) the amino acid corresponding to position 1610 of SEQ ID NO: 2 is mutated from N to C; and (iii) the amino acids corresponding to Q1624 to R1641 of SEQ ID NO: 2 are deleted. In another aspect, there is provided an ADAMTS13 polypeptide substrate that is from 50 to 75 amino acids in length and has an amino acid sequence that is substantially similar to part of the von Willebrand factor A2 domain sequence set forth in SEQ ID NO:2, with one or more of the following modifications: (i) the amino acid corresponding to position 1599 of SEQ ID NO: 2 is mutated from Q to K; (ii) the amino acid corresponding to position 1610 of SEQ ID NO: 2 is mutated from N to C; (iii) the amino acid corresponding to position 1629 of SEQ ID NO: 2 is mutated from G to E; and (iv) the amino acids corresponding to G1631 to R1641 of SEQ ID NO: 2 are deleted. | 05-28-2015 |
| 20150291997 | METHODS AND DEVICES FOR DETECTION OF RESISTANCE TO AN ENZYME INHIBITOR - Embodiments described herein relate to assay methods and kits for detecting resistance of any enzyme to its inhibitor due to functional alteration of the enzyme, comprising, conducting two or more reactions with two or more reagent mixes optionally containing substrates for the enzyme. The mixes are substantially similar, except that one contains no enzyme inhibitor whereas the others contain an enzyme inhibitor being tested for resistance. The ratio of the signal from the reaction with an inhibitor to that from a reaction without an inhibitor is used to indicate whether the enzyme is resistant to the enzyme inhibitor and also determine the susceptibility or resistance of the enzyme to various inhibitors and further identify enzyme variants. Embodiments further relate to assay methods comprising only two reactions—one conducted in a mix containing the inhibitor and the other without the inhibitor. Further included are devices for conducting such assays. | 10-15-2015 |
| 20150293106 | DIAROMATIC AMINO ACID SUBSTRATE FOR CATHEPSIN DETECTION - The present invention relates to a diaromatic amino acid substrate for cathepsin detection. When the substrate used for cathepsin detection is a polymer resulting from the binding of a protease-preferred amino acid and quencher to a diaromatic amino acid, cathepsin can be detected sensitively and accurately by fluorescence because cathepsin causes the protease-preferred amino acid and quencher to dissociate from the diaromatic amino acid, and fluorescence is recovered during this time. | 10-15-2015 |
| 20150346202 | STABILIZED CHEMILUMINESCENT SYSTEM - The present invention relate to formulations, systems, kits and methods for chemiluminescence assays to detect the catalytic activity of a peroxidase. The present invention provides formulations and methods for chemiluminescence reactions that have significantly improved signal duration, which signal lasts for hours. | 12-03-2015 |
| 20160002619 | LABELED GLUTAMINASE PROTEINS, ISOLATED GLUTAMINASE PROTEIN MUTANTS, METHODS OF USE, AND KIT - The present invention relates to a labeled glutaminase (GLS) protein comprising a GLS protein and a fluorescent reporter group attached to the GLS protein, wherein the fluorescent reporter group is attached to the GLS protein within the glutaminase domain pfam04960 of GLS. The present invention also relates to isolated glutaminase protein mutants. Also disclosed is a method of screening for compounds that allosterically bind to a glutaminase protein. The present invention also relates to a method of identifying compounds that inhibit or stabilize tetramer formation of glutaminase protein. The present invention further relates to a screening kit for compounds that inhibit or stabilize tetramer formation. | 01-07-2016 |
| 20160084860 | MULTIFUNCTIONAL PROTEIN MOLECULAR WEIGHT LADDERS - Multifunctional molecular weight protein ladders and methods of making thereof are disclosed herein that are useful for determining the molecular weight of a test protein and/or the relative mass or amount of the test protein in a protein separation assay, such as gel electrophoresis or western blotting. Also included are compounds of Formula I (e.g., mono acetylated MP-11 NHS ester) that may be used to label purified proteins of the protein ladder. The MP-11 label protein ladder can be detected on a blotting membrane by exposing the microperoxidase to a suitable substrate, such as a chromogenic substrate or a chemiluminescent substrate. | 03-24-2016 |
| 20160146814 | Fluorogenic/Fluorescent Probes Derivative from Sulfoxanthene, and Use Thereof - The invention relates to fluorescent/fluorogenic probes of formula (I″) or (II″): | 05-26-2016 |
| 20160376630 | PHOSPHORESCENCE-BASED HYDROGEN PEROXIDE ASSAY FOR THE DETECTION OF HYDROGEN PEROXIDE IN HUMAN SERUM AND WATER SAMPLES - The present invention relates to a method for determining an amount of a peroxide in a sample, wherein the method comprises the steps of: —providing a sample, —contacting the sample with a terbium(III) benzene dicarboxylic acid complex, and —determining the luminescence of the terbium(III) benzene dicarboxylic acid complex. | 12-29-2016 |
