| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 435700600 | Involving a modified enzyme (e.g., abzyme, recombinant, chemically altered, etc.) | 16 |
| 20080311592 | Allosteric Control of Proteins by Manipulating Mechanical Tension - A method of altering the conformation of a polypeptide having a known three-dimensional structure is described. The method comprises attaching a first end of a polymer to a first portion of the polypeptide, attaching a second end of the polymer to a second portion of the polypeptide, and altering the mechanical tension of the polymer, thereby altering the conformation of the polypeptide. The alteration of the conformation of the polypeptide may increase or decrease the binding affinity of the polypeptide for a substrate bound by the polypeptide, or alter the catalytic rate of an enzyme. Typically, the polymer is a polynucleotide or polypeptide. | 12-18-2008 |
| 20090275053 | CELL-BASED PCSK9 SCREENING ASSAY - The present invention includes a PCSK9 activity inhibition assay system, kits, compositions and methods. The present invention includes a cell having a first vector capable of expressing a catalytic fragment of PCSK9, a second vector capable of expressing a prodomain of PCSK9 and a V5 protein with a detectable label. The V5 protein forms a fusion protein with the prodomain of PCSK9 and wherein cleavage of the prodomain by the catalytic fragment of PCSK9 releases a detectable signal. | 11-05-2009 |
| 20090286261 | METHOD FOR ASSAYING COMPOUNDS OR AGENTS FOR ABILITY TO DISPLACE POTENT LIGANDS OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE - An exemplary embodiment may be directed to a fluorescence polarization assay that screens compounds or agents for their affinity to hematopoietic prostaglandin D synthase (H-PGDS) based on their ability to displace a fluorophore-containing detection analyte bound to an enzyme comprising the primary amino acid sequence of H-PGDS. Another exemplary embodiment utilizes an enzyme having a maltose binding protein amino-acid sequence fused with an N-terminus of the enzyme. | 11-19-2009 |
| 20100184090 | Method for Generating Stable Cell Lines Expressing High Levels of a Protein of Interest - This invention relates to industrial production of proteins. More specifically, the invention relates to a method for obtaining cells that stably express a protein of interest, even when cultivated in the absence of selective pressure. DHFR is used as a surrogate marker. The transfected cells are not selected based on resistance to a toxic compound, but based on fluorescence as measured by FACS using fluorescent MTX. | 07-22-2010 |
| 20100190187 | Screening Assays for Antagonists and Analyses of Cardiac Hypertrophy - Various embodiments of the present invention provide methods for screening for candidate heart failure compounds employing screening assays effective in identifying agonists or antagonists or ligands of vitamin D receptor mediated pathways implicated in heart failure. Methods are provided for the screening of test compounds that can specifically bind to the vitamin D receptor. Methods for screening for a test compound which modulates the activity of a VDR for the treatment of heart failure, wherein the method comprises: (a) contacting a test compound with VDR in a reaction mixture, wherein the reaction mixture conditions permits the test compound to bind to a VDR, including membrane VDR and nuclear VDR. The binding between the test compound and the VDR is compared to a reference such as Vitamin D3. The modulation of biomarkers after a test compound has bound and activated a VDR are also measured and compared to samples in the absence of test compound. | 07-29-2010 |
| 20110081660 | Method for Detecting Misfolded Proteins and Prions - The invention provides methods and kits for detecting conformationally altered proteins, such as prions or other proteins associated with disease states, in a sample. The methods comprise selectively capturing and separating complexes of peptide and conformationally altered protein from substances that interfere with detection of such complexes, and preferably amplification of the detection signal b addition of a second double-labeled peptide. | 04-07-2011 |
| 20110124011 | METHOD FOR DETERMINING SUMOYLATION - The present invention relates to a method for determining SUMOylation and utilizing said SUMOylation patterns for identifying specific interaction between different binding partners. In another aspect, the present invention relates to systems allowing the determination of SUMOylation and for determining specific interaction between binding partners. Furthermore, the present invention relates to vectors and proteins relating to SUMOylation. | 05-26-2011 |
| 20110136140 | DIAGNOSIS OF SYSTEMIC DISEASES - It has been found that the pattern of recognized topo I epitopes is different between dcSSc, IcSSc and SLE patients. Fragment F4 (amino acid (AA) 450-600) was recognized by all patients tested. Fragment F1 (AA 5-30) and Fragment F8 (AA 350-400) represent characteristic epitopes for dcSSc and SLE, respectively. The invention relates to diagnostic uses and methods as well as kits for use in diagnosis. | 06-09-2011 |
| 20110201024 | COMPOSITIONS COMPRISING A DEHALOGENASE SUBSTRATE AND A FLUORESCENT LABEL AND METHODS OF USE - A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein. | 08-18-2011 |
| 20120003667 | ELECTROPHORETIC TAG-BASED IN VITRO ASSAY TO QUANTIFY DIMERIZATION OF P66 AND P51 SUB-UNITS OF HIV-1 REVERSE TRANSCRIPTASE (RT) - Methods for measuring hetero- and homodimerization of HIV reverse transcriptase (HIV RT) are provided using pairs of tagged probes and cleaving probes. The methods can be used, for example, to screen for modulators of HIV RT dimerization. Also provided are methods of determining whether a compound modulates HIV RT. Further provided are methods of determining whether a compound modulates formation of a complex between a p66 and p51, p66 and p66, or p51 and p55 subunits polypeptides of HIV RT. | 01-05-2012 |
| 20120107836 | DEVELOPMENT OF FLUORESCENTLY P-LOOP LABELLED KINASES FOR SCREENING OF INHIBITORS - The present invention relates to a kinase labelled at an amino acid naturally present or introduced in the P-loop of said kinase, wherein said labelling is effected at a free thiol or amino group of said amino acid and said label is (a) a thiol- or amino-reactive fluorophore sensitive to polarity changes in its environment; or (b) a thiol-reactive spin label, an isotope or an isotope-enriched thiol- or amino-reactive label, such that said fluorophore, spin label, isotope or isotope-enriched label does not inhibit the catalytic activity and does not interfere with the stability of the kinase. The invention furthermore relates to a method of screening for kinase inhibitors, a method of determining the kinetics of ligand binding and/or of dissociation of a kinase inhibitor and a method of generating mutated kinases suitable for the screening of kinase inhibitors using the kinase of the present invention. | 05-03-2012 |
| 20120258470 | COMPOSITIONS COMPRISING A DEHALOGENASE SUBSTRATE AND A RADIONUCLIDE AND METHODS OF USE - A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein. | 10-11-2012 |
| 20120329072 | Modification-Dependent Activity Assays - Disclosed herein are methods, systems and kits to measure the presence and/or activity of recombinant polypeptides comprising a modification. | 12-27-2012 |
| 20150355172 | ADP-RIBOSE DETECTION REAGENTS - The present disclosure described fusion proteins between ADP-ribose binding domains and antibody Fc domains. This new class of reagents has considerable value as detection agents in assays designed at examining the biology of ADP-ribose. | 12-10-2015 |
| 20160054322 | HOMOGENOUS THERMAL SHIFT LIGAND BINDING ASSAY - Disclosed are methods for detecting and quantitatively measuring a binding property of a compound to a target macromolecule, wherein the target macromolecule is subject to denaturation and is linked to a labeling peptide, such as a short enzyme fragment. The method uses a fluid mixture comprising (i) a chimeric molecule comprising a target macromolecule linked to the labeling peptide and (ii) a compound being measured for binding to the target macromolecule, wherein said target macromolecule is subject to denaturation. After allowing for binding of the compound (e.g. a small molecule inhibitor of the target macromolecule), one detects a signal from the labeling peptide, such as by enzyme fragment complementation. This signal indicates a differential between denatured and non-denatured target macromolecules and thereby indicates a differential between target macromolecules not bound to the compound and target macromolecules bound to the compound, respectively. | 02-25-2016 |
| 20160186158 | VACCINES COMPRISING LEISHMANIA POLYPEPTIDES FOR THE TREATMENT AND DIAGNOSIS OF LEISHMANIASIS - Compositions and methods for preventing, treating and detecting leishmaniasis are disclosed. The compositions generally comprise polypeptides comprising | 06-30-2016 |
