| Entries |
| Document | Title | Date |
|---|
| 20080198447 | Microscope with Adjustable Stage - Systems and techniques relating to a microscope with an adjustable stage are described. A microscope includes a base, a support arm attached to and extending upwardly from the base, a head attached to the support arm, the head including a lens, and an eyepiece attached to the head. A stage is releasably attachable to the support arm between the head and the base at multiple locations, providing multiple working distances between a lower surface of the head and an upper surface of the stage. When the stage is attached to the support arm at a location, a working distance between the lower surface of the head and the upper surface of the stage is further adjustable to an either greater or lesser working distance. In another embodiment, microscopic and macroscopic viewing can both be provided using a lens changer with multiple lens positioned radially about an axis of rotation. | 08-21-2008 |
| 20080198448 | FLUORESCENCE MICROSCOPE HAVING AN ILLUMINATION DEVICE - An arrangement for fluorescence microscopic examination of specimens includes: a fluorescence microscope; an illumination device that includes: a housing including an interface configured to optically couple the housing and the fluorescence microscope; a plurality of light-emitting diodes disposed in the housing; a respective collector disposed downstream of each of the light emitting diodes and configured to generate a directed light flux; and at least one dichroic splitter disposed in the housing, the at least one splitter and the light-emitting diodes being spatially disposed with respect to one another so that the directed light fluxes are combinable via the at least one splitter into a common illumination beam path directed onto the interface; and a logical control device common to the fluorescence microscope and the illumination device. | 08-21-2008 |
| 20080198449 | Laser microscope - It is possible to change a focal position or spot diameter of an optical stimulation laser beam without causing any misalignment of the optical axis, thus precisely applying optical stimulation to a desired position or region on a specimen. The invention provides a laser microscope comprising an observation light path for guiding an observation laser beam; an optical stimulation light path for guiding an optical stimulation laser beam; and a light-path combining unit for combining these light paths; and the laser microscope also comprises, in at least the optical stimulation light path, a focal-position adjusting unit for adjusting a focal position of the laser beam; an optical-axis misalignment detector for detecting an amount of misalignment of an optical axis between the focal-position adjusting unit and the light-path combining unit; and an alignment unit for adjusting an optical axis position on the basis of the amount of misalignment of the optical axis detected by the optical-axis misalignment detector. | 08-21-2008 |
| 20080212173 | Illumination Optical Apparatus and Optical Apparatus - There are provided an illumination optical apparatus and an optical apparatus using this illumination optical apparatus that are capable of efficiently collecting light emitted from a light-emitting section for illumination with less illumination nonuniformity, without having to employ a complicated structure. There are provided a light-emitting sections; a lens system for converting a diverging beam emitted from the light-emitting section into a beam of collimated light; an afocal optical system for adjusting the cross-sectional area of a beam of collimated light obtained by the lens array; a fly-eye lens for forming a plurality of light-source images from the collimated light whose cross-sectional area is adjusted by the afocal optical system; and a Koehler illumination optical system that uses the plurality of light-source images formed by the fly-eye lens as a light source. | 09-04-2008 |
| 20080218850 | Light Profile Microscopy Apparatus and Method - An apparatus and method allowing an optimized illumination in a light profile microscope by excitation of a sample with an elliptically collimated beam. The beam, which is typically supplied by a laser is collimated with unequal beam waist radii (and Rayleigh ranges) along major and minor axes orthogonal to a propagation direction, and approximates a plane sheet of illumination. The plane sheet of illumination is aligned with a thinnest width dimension thereof along the optic axis of the microscope objective, and with a center thereof at the object plane of the objective. Excitation light in a test sample is thereby confined to within a narrow thickness of the object plane of the objective lens, which minimizes out-of focus light in the image. The major axis width of the plane illumination sheet is typically a factor of ten or more greater than the minimum width, allowing a large area of the test sample to be illuminated and imaged. This excitation arrangement optically emulates the operation of micro-toming a thin cross section of a material for analysis, and provides optimum resolution and field in a light profile microscope. | 09-11-2008 |
| 20080225388 | OPTICAL SCANNING OBSERVATION APPARATUS - The invention provides a compact optical scanning observation apparatus having an internal focusing mechanism and which is suitable for in-vivo observation of animals. The optical scanning observation apparatus comprises a light source for illuminating a sample; an objective optical system for focusing illumination light onto the sample; a detection-light splitting device for splitting off collected detection light from the illumination light; a detection-light focusing optical system for focusing the split-off detection light; a detector for detecting the focused detection light; a focus scanning device disposed between the detection-light splitting device and the objective optical system; and a lateral-direction scanning device, disposed between the focus scanning device and the objective optical system, for scanning the illumination light from the light source on the sample, in substantially orthogonal directions with respect to the optical axis. The focus scanning device includes a focusing optical system formed of at least a positive lens group and a negative lens group, and a lens driving device for moving at least one lens group included in the focusing optical system to change a working distance of the objective optical system. | 09-18-2008 |
| 20080239475 | CONFOCAL MIMCROSCOPE SYSTEM - A confocal microscope system capable of observing a bright field image and a fluorescent image together with a confocal image can be achieved with a simple configuration. The confocal microscope system, for observing the bright field image, the fluorescent image, the confocal image acquired from the observation sample, is characterized in comprising: a confocal scanner unit having at least a microlens array disc and a pin hole array disc, for scanning a face of the observation sample by a plurality of beam spots, and a relay lens connected to a camera for acquiring observed images; a microscope for holding the observation sample and illuminating an observation light for observing the bright field image and an excitation light for observing the fluorescent image on the observation sample, and having a port part for outputting the observation light acquired from the observation sample to the confocal scanner unit; and a detour light path unit selectively inserted between the port part of the microscope and the confocal scanner unit for branching the observation light from the observation sample and guiding the branched observation light to the relay lens in the confocal scanner unit. | 10-02-2008 |
| 20080259443 | CONTACT MICROSCOPE USING POINT SOURCE ILLUMINATION - The embodiments of the invention include a microscope having a transparent specimen holder and a digital imaging device positioned within the transparent specimen holder. The digital imaging device can include a wireless transmitter. The transparent specimen holder can have a top surface and a bottom surface, wherein the transparent specimen holder is completely transparent between the top surface and the bottom surface. Thus, the transparent specimen holder is completely transparent above and below the digital imaging device. Furthermore, a processor is operatively connected to the digital imaging device, wherein the processor produces an image of a specimen positioned on the specimen holder. A display is operatively connected to the processor, wherein the display displays the image. | 10-23-2008 |
| 20080259444 | CONTACT MICROSCOPE USING POINT SOURCE ILLUMINATION - The embodiments of the invention include a microscope having a transparent specimen holder and a digital imaging device positioned within the transparent specimen holder. The digital imaging device can include a wireless transmitter. The transparent specimen holder can have a top surface and a bottom surface, wherein the transparent specimen holder is completely transparent between the top surface and the bottom surface. Thus, the transparent specimen holder is completely transparent above and below the digital imaging device. Furthermore, a processor is operatively connected to the digital imaging device, wherein the processor produces an image of a specimen positioned on the specimen holder. A display is operatively connected to the processor, wherein the display displays the image. | 10-23-2008 |
| 20080266658 | OPTICAL SYSTEM FOR CELL IMAGING - A microscope system ( | 10-30-2008 |
| 20080278801 | Laser scanning microscope - A laser scanning microscope is capable of separating the fluorescence signals of different fluorophores in accurate unmixing realized by eliminating positional pixels shifts between different fluorescence images obtained through irradiation of different-wavelength laser lights. The microscope includes a laser light source capable of emitting a wavelength-changeable laser light, a correction amount determination unit that determines a correction amount for correcting an optical axis shift of the laser light, an optical axis adjusting unit that adjusts an optical axis, a scanning unit that performs two-dimensional scanning, an objective lens that focuses the laser scanning light to a specimen and fluorescence emitted from the specimen, a light detector that detects the fluorescence, and a control unit that changes the wavelength of the laser light synchronously with the scanning by the scanning unit while controlling the optical axis adjusting unit on the basis of the correction amount determined by correction amount determination unit. | 11-13-2008 |
| 20080304146 | Microscope System for Fcs Measurements - A microscope system for conducting FCS measurements. The system includes an illuminating light source configured to emit an illuminating light at an illuminating wavelength. A target light source is provided and configured to emit a target light for marking an FCS volume in a sample volume at a target wavelength. The target wavelength differs from the illuminating wavelength. The system further includes a plurality of optical elements configured to direct the illuminating light and the target light onto the sample volume. | 12-11-2008 |
| 20080310017 | MICROSCOPE FOR OBSERVING A SAMPLE IN THE BRIGHT FIELD ILLUMINATION BY TRANSMITTED LIGHT OR IN FLUORESCENCE-CONTRAST EPI-ILLUMINATION - A microscope for observing an object selectably using the bright-field transmitted light contrast procedure or the incident-light fluorescence contrast procedure. During the bright-field transmitted light contrast procedure, an illumination beam path is directed from a bright-field light source to the object and an imaging beam path is directed from the object through the microscope objective into the microscope tube, which includes a fluorescence unit that includes a fluorescence excitation light source, an illumination optical system, a filter set having at least one excitation filter and at least one emission filter, as well as a beamsplitter. The fluorescence unit is mounted movably, so that in a first end position of movement the beamsplitter and the emission filter are out of the imaging beam path of the microscope and in a second end position of movement they are in the imaging beam path between the microscope objective and the microscope tube. | 12-18-2008 |
| 20090002812 | MICROSCOPE WITH CENTERED ILLUMINATION - A microscope comprising a main objective having a variable focal length and comprising an illuminating unit including a light source and an illuminating optical system for generating an illuminating beam path directed onto the object plane and extending outside the main objective. A unit is provided for centering the illumination dependent on a variation of the focal length of the main objective, and the illuminating optical system is mounted at least in part in a laterally shiftable manner for centering the illumination. In particular, the illuminating optical system has a diaphragm that is mounted in a laterally shiftable manner. | 01-01-2009 |
| 20090015912 | Total Internal Reflectance Fluorescence (TIRF) Microscope - A total internal reflection fluorescent (TIRF) microscope has a conjugate lens ( | 01-15-2009 |
| 20090027769 | IMMERSION MICROSCOPE OBJECTIVE AND LASER SCANNING MICROSCOPE SYSTEM USING SAME - An immersion microscope objective formed of thirteen or fewer lens elements includes, in order from the object side, first and second lens groups of positive refractive power, a third lens group, a fourth lens group having negative refractive power with its image-side surface being concave, and a fifth lens group having positive refractive power with its object-side surface being concave. The first lens group includes, in order from the object side, a lens component that consists of a lens element of positive refractive power (when computed as being in air) and a meniscus lens element having its concave surface on the object side. Various conditions are satisfied to ensure that images of fluorescence, obtained when the immersion microscope objective is used in a laser scanning microscope that employs multiphoton excitation to observe a specimen, are bright and of high resolution. Various laser scanning microscopes are also disclosed. | 01-29-2009 |
| 20090046360 | Raster scanning light microscope with line pattern scanning and applications - Raster scanning light microscope with line pattern scanning with at least one illumination module, in which the means to achieve a variable partition of the laser light into least two illumination channels are envisioned and joint illumination of a sample takes place at the same or at different areas of the sample. | 02-19-2009 |
| 20090052021 | Microscopic Cell Observation and Inspection System Using a Plurality of Observation Methods - The invention relates to a microscopic cell observation and inspection system that uses a total internal reflection cell illuminator that is capable of freely changing an observation position without recourse to any special slide glass, makes sure high SN-ratio observation and facilitates sample manipulation, thereby making high-sensitivity, fast detection of a lot of cell reactions on the same slide glass. While, in response to a command from personal computer ( | 02-26-2009 |
| 20090052022 | Operating unit for optical imaging devices - The invention relates to an operator-controlled device ( | 02-26-2009 |
| 20090059362 | Microscope and three-dimensional information acquisition method - An optical system is provided to include an optical unit with an optical axis extending through a light transmissive sample embedded in a transparent substrate, to focus on the sample embedded in the substrate and to scan the sample according to the main plane of the transparent substrate. The optical axis extends under an angle unequal to zero relative to the normal of the main plane of the transparent substrate, in order to perform a volumetric observation of a sample by obtaining information items focused in all thickness directions within the sample, at a high speed, without requiring any movement along the thickness direction of the sample. | 03-05-2009 |
| 20090067042 | In-vivo examination apparatus - When carrying out in vivo examination of a living organism, the behavior in the interior thereof is continuously observed with clear images. Provided is an in-vivo examination apparatus | 03-12-2009 |
| 20090073554 | SCANNING EXAMINATION APPARATUS - A scanning examination apparatus | 03-19-2009 |
| 20090073555 | SCANNING MICROSCOPE AND METHOD FOR MANIPULATING SAMPLES BY MEANS OF A MANIPULATING LIGHT BEAM IN A SCANNING MICROSCOPE - A scanning microscope for manipulating a sample, the microscope, having a first light source, a second light source, a beam deflector, and an optical device. The first light source is configured to emit an illuminating light beam that follows an illuminating beam. A second light source is configured to produce a manipulating light beam which has a manipulating beam focus and follows a manipulating beam path. The beam deflection device is configured to guide the illuminating light beam and the manipulating beam focus over or through the sample. The optical device is disposed downstream of the second light in the manipulating beam and is configured to modify the size of the manipulating beam focus. | 03-19-2009 |
| 20090086315 | ARRANGEMENT FOR EXAMINING MICROSCOPIC PREPARATIONS WITH A SCANNING MICROSCOPE, AND ILLUMINATION DEVICE FOR A SCANNING MICROSCOPE - The arrangement for examining microscope preparations with a scanning microscope comprises a laser ( | 04-02-2009 |
| 20090097108 | Confocal scanning microscope having optical and scanning systems which provide a handheld imaging head - A confocal microscope having a scanning head in the form of a hand piece ( | 04-16-2009 |
| 20090097109 | CONFOCAL MICROSCOPE - To provide a confocal microscope for obtaining an extended image by easily determining the capturing range of an observation image without being aware of the shape of a sample to be observed, the confocal microscope comprises confocal image generating unit for generating a confocal image, first counting unit for counting a first number of pixels having a predetermined brightness level or lower among the brightness levels of the pixels of the confocal image, second counting unit for counting a second number of pixels obtained by extracting only a pixel that matches a predetermined condition for the confocal image, and boundary determining unit for detecting a boundary by determining whether or not the observation surface is within the image capturing range based on the first and the second numbers of pixels. | 04-16-2009 |
| 20090103175 | DEVICE AND METHOD FOR HIGH-INTENSITY HOMOGENEOUS ILLUMINATION WITH MINIMIZED LIGHT REFLECTION FOR USE IN MICROSCOPES - The invention relates to a device for high-intensity uniform illumination with minimal light reflection for use in reflective-type microscopes. The invention is characterised by a light source ( | 04-23-2009 |
| 20090109525 | OPTICAL MICROSCOPE - An optical microscope, which has a light source, for observing a sample by illuminating light from the light source on the sample includes a second light source that is different from the light source and is insertable/removable in/from the optical path of the optical microscope. The second light source is configured to have the peak of an emission spectrum only in a wavelength range of 400 to 490 nm. | 04-30-2009 |
| 20090109526 | Illumination Device For A Light Microscope And Light Microscope With Such An Illumination Device - An illumination device for a light microscope with an objective, in particular for a stereo-microscope, illuminates an object plane of the light microscope. An illumination beam path of the illumination device is defined by a light source, a field diaphragm, illumination optics with at least one illumination lens, and at least one deflection element. The object plane of the microscope is obliquely illuminated with incident illumination, wherein an axis of the illumination beam path forms an angle β greater than 0° with an optical axis of the objective. The field diaphragm, the illumination optics and the deflection element are arranged and orientated relative to one another such that a diaphragm plane which is defined by the field diaphragm, an illumination lens plane defined by the illumination lens, and an image plane of the image of the field diaphragm, which is produced by the illumination optics, without deflection by the deflection element, intersect at least approximately along a common straight line and that the image plane of the image of the field diaphragm which is produced by the illumination optics and which is deflected by the deflection element, runs in or parallel to the object plane. Sharp, undistorted imaging of the field diaphragm into the object plane or into a plane running parallel thereto is achieved. | 04-30-2009 |
| 20090128898 | MICROSCOPY METHOD AND MICROSCOPE - A microscopy method is provided for generating an image of an image field passing in a predetermined depth of a sample to be examined, comprising a plurality of illumination steps, in which a part of the image field is in each case illuminated with a focused illumination beam bundle, which effects the generation of sample radiation on account of an interaction with the sample, detection steps, in which the sample radiation generated is detected, and an evaluation step, in which the image is generated on the basis of the sample radiation detected, wherein a first and second detection step are carried out during each illumination step, wherein sale radiation generated at the focus and outside the focus is detected in the first detection step and a smaller proportion of the sample radiation generated at the focus than in the first detection step and also sample radiation generated outside the focus are detected in the second detection step, and wherein the sample radiation detected in the second detection step is used in the evaluation step to reduce the proportion outside the focus in the sample radiation detected in the first detection step. | 05-21-2009 |
| 20090135476 | LIGHT SOURCE APPARATUS AND LASER SCANNING MICROSCOPE - Beth of highly stable light having the same optical performance and fast-modulated light are emitted simply and inexpensively. A light source apparatus is provided, which includes semiconductor laser elements that emit laser light according to an inputted current signal, a light receiving element that receives the laser light emitted from the semiconductor laser element, and a controller that controls light emission of the semiconductor laser element, wherein the controller includes a first semiconductor laser element drive circuit that outputs a current signal to the semiconductor laser elements according to an instruction signal, a second semiconductor laser element drive circuit that adjusts the current signal on the basis of light quantity of the laser light received by the light receiving element, and outputs the adjusted current signal to the semiconductor laser element, and a circuit switching section that switches between the first and the second semiconductor laser element drive circuits according to an instruction signal. | 05-28-2009 |
| 20090153955 | Scanning microscope - A scanning microscope includes a source of illumination light; a scanner scanning the illumination light in a two-dimensional direction crossing a light axis; a lens irradiating the illumination light to a sample, and collecting return light from the sample; a focusing position adjuster adjusting a focal position in a light axis direction; and a light detector detecting collected light. A storage section stores the intensity of detected light, and positional information of an irradiating position of the illumination light set by the scanner and the focusing position adjuster. An image processor acquires images parallel to the light axis based on the intensity of return light and the stored positional information, and processes the images to detect a moving distance along a light axis direction of an area of the sample. The focusing position adjuster is controlled to correct a light condensing position of the illumination light. | 06-18-2009 |
| 20090153956 | MICROSCOPE ILLUMINATION APPARATUS - A microscope illumination apparatus includes a light source, a collector lens for converting a light ray from the light source into an almost collimated light flux, a field stop provided in the almost collimated light flux from the collector lens, a field lens for converting a light ray from the field stop into an almost collimated light flux, and a condenser lens for collecting the almost collimated light flux from the field lens on a sample surface. The distance between the condenser lens and the field lens is variable. | 06-18-2009 |
| 20090161205 | BOX-TYPE MOTOR-OPERATED MICROSCOPE - A box-type motor-operated microscope has a motor-operated microscope section having a transmitting illumination optical system, an electric stage, and an image forming optical system; and a housing. The housing includes a fixed housing and a moving housing. The moving housing is movable parallel to an oblique direction with respect to the fixed housing while holding optical elements arranged above the electric stage so that the specimen vessel placed on the electric stage is made replaceable, the motor-operated microscope section is sealed and light-blocked in cooperation with the fixed housing, and the optical axis of the transmitting illumination optical system is practically aligned with that of the image forming optical system. | 06-25-2009 |
| 20090161206 | Point Source Apparatus and Method of Manufacture Thereof - A point electromagnetic radiation source apparatus ( | 06-25-2009 |
| 20090161207 | Optical scanning microscope - Microscope, in particular an optical scanning microscope with illumination of a specimen via a beam splitter, which is arranged in an objective pupil and includes at least a reflecting first portion and at least a transmitting second portion, whereby the reflecting portion serves to couple in the illumination light and the transmitting portion serves to pass the detection light in the detection direction or the transmitting portion serves to couple in the illumination light and the reflecting portion serves to couple out the detection light, with a first scanning arrangement. Means are provided in the detection light path for the overlay of at least one further scanning arrangement for illumination and detection. | 06-25-2009 |
| 20090161208 | Method and Configuration for Optically Detecting an Illuminated Specimen - A configuration for the optical detection of a specimen, wherein the specimen or at least part of the specimen is scanned by means of linear illumination by scanning means, means for linear beam shaping of the illuminating light are provided, and the illuminating light has a preferably periodic structure in at least one spatial direction in that means for generating the structure are disposed in the illuminating beam path, light coming from the specimen is detected and images of the specimen are generated therefrom, at least one optical sectional image through the specimen and/or one image with increased resolution is/are calculated from the images, and means for generating the structure are disposed downstream of the scanning means in the direction of the illumination. | 06-25-2009 |
| 20090161209 | MICROSCOPE - Provided are an illuminating optical system which illuminates a sample, an illumination-side pupil modulating device which is arranged on a side of the illuminating optical system, an illumination-side turret which holds the illumination-side pupil modulating device, an illumination-side-turret revolving mechanism which revolves the illumination-side turret to move the illumination-side pupil modulating device along an orbital circumference on a plane perpendicular to an optical axis, a relaying optical system which relays a pupil of an objective lens; an imaging-side pupil modulating device which is arranged on a side of the relaying optical system, an imaging-side turret which holds the imaging-side pupil modulating device, and an imaging-side-turret revolving mechanism which revolves the imaging-side turret to move the imaging-side pupil modulating device along the orbital circumference on a plane perpendicular to the optical axis. | 06-25-2009 |
| 20090168157 | Laser scanning apparatus and laser scanning microscope - To provide a laser scanning apparatus and a laser scanning microscope capable of securely conducting a condition setting at the time of laser scanning while suppressing a damage on a plane to be irradiated. Accordingly, a laser scanning apparatus includes a light deflecting unit disposed in a light path of laser light directed toward a plane to be scanned, user interfaces through which operational contents of the light deflecting unit are designated by a user, generating units generating driving signals of the light deflecting unit in accordance with the designated operational contents, and testing units test-driving the light deflecting unit with the driving signals while keeping the laser light off and measuring the operational contents of the light deflecting unit during the driving. | 07-02-2009 |
| 20090168158 | Method and Configuration for the Optical Detection of an Illuminated Specimen - A method for the optical detection of an illuminated specimen, wherein the illuminating light impinges in a spatially structured manner in at least one plane on the specimen and several images of the specimen are acquired by a detector in different positions of the structure on the specimen, from which images an optical sectional image and/or an image with enhanced resolution is calculated. The method includes generating a diffraction pattern in the direction of the specimen in or near the pupil of the objective lens or in a plane conjugate to the pupil. A structured phase plate with regions of varying phase delays is dedicated to the diffraction pattern in or near the pupil of the objective lens or in a plane conjugate to said pupil. The phase plate is moved in order to set different phase angles of the illuminating light for at least one diffraction order on the specimen, wherein diffraction orders are selected to advantage via a movable diaphragm which is disposed in or near the pupil of the objective lens or in a plane conjugate to said pupil. | 07-02-2009 |
| 20090195866 | MICROSCOPE - A microscope has an objective lens, an imaging lens projecting light passing through the objective lens to form an image of a specimen, an image sensor located at an imaging position where the image of the specimen is formed by the imaging lens, an illumination light source, and a reflecting fluorescence illumination optical system including a dichroic mirror introducing light from the illumination light source into an optical path on the objective lens side, illuminating the specimen with the light. In this case, the microscope further has a relay optical system in which an intermediate image of the specimen is formed between the objective lens and the imaging lens and is relayed to the imaging lens, and the dichroic mirror of the reflecting fluorescence illumination optical system is located between a pupil position conjugate with a pupil position of the objective lens, formed between the relay optical system and the imaging lens, and the relay optical system. | 08-06-2009 |
| 20090195867 | INVERTED MICROSCOPE - An inverted microscope for the high-contrast imaging of objects includes multiple objectives configured to be disposable in an imaging beam path. Each objective has a respective objective pupil associated therewith. A lens system is also provided for generating respective intermediate images of the respective objective pupils. A modulator disposing device, which includes a plurality of modulators, is configured to dispose the modulators at predetermined respective locations on an optical axis corresponding to the locations of the respective intermediate images of the respective objective pupils so as to contrast an object image. | 08-06-2009 |
| 20090195868 | SOLID STATE FLUORESCENCE LIGHT ASSEMBLY AND MICROSCOPE - An illumination system for a fluorescence microscope is provided. The illumination system includes a carriage removably receivable within the microscope and a plurality of filter cubes movably arranged on the carriage, wherein each filter cube is moveable between an active position and an inactive position. Each filter cube includes a housing having first and second openings and a solid state light source secured to the housing. The solid state light source emits light when the filter cube is moved into the active position. Each filter cube further comprises at least one optical filter disposed within the housing, wherein the optical filter corresponds to the solid state light source. | 08-06-2009 |
| 20090219614 | MICROSCOPE APPARATUS - A microscope apparatus including a light source device, a storage table, and a setting controller is provided. The light source device includes a light intensity adjuster which changes light intensity of an illumination light of plural different wavelength regions selectively extracted from light emitted from a light source, and thereby emitting the illumination light including light of the plural different wavelength regions in such a manner that a light intensity ratio of light of one wavelength region to light of another wavelength region is variable. The storage table stores therein set values of the light intensity adjuster for each of combination patterns of the wavelength regions of the illumination light emitted from the light source device. The setting controller makes the light source device change a setting of the light intensity adjuster based on the set value stored in the storage table according to wavelength designation information which designates a combination pattern of the wavelength regions. | 09-03-2009 |
| 20090231692 | Laser scan confocal microscope - Fluorescence is generated from an irradiated point on an inspection surface of a sample ( | 09-17-2009 |
| 20090273829 | Observing Device - The observing apparatus is being equipped with an image-forming optical system which forms an image of light emitted from a specimen, an imaging unit which picks up the image of the specimen formed by the image-forming optical system, and an illuminating unit which illuminates the specimen with a surface illuminant in which bright areas and dark areas are arranged alternately in order to provide an observing apparatus suitable to observe a transparent specimen with a wide field of view. If the position of the surface illuminant and the pitch of contrasting are set properly, each partial area of the specimen is illuminated obliquely at a small angle by each bright area of the surface illuminant. Therefore, the imaging unit can acquire a dark-field observation image of each partial area. | 11-05-2009 |
| 20090273830 | FOCUS DETECTION APPARATUS, MICROSCOPE - A focus detection apparatus is provided with a light source | 11-05-2009 |
| 20090279169 | Relating to Scanning Confocal Microscopy - An assembly ( | 11-12-2009 |
| 20090284833 | MICROSCOPE ILLUMINATION DEVICE - A microscope illumination device comprising: a light source; an objective for illuminating a specimen; a collector lens for converting light from the light source into parallel light; a fly-eye lens placed near to rear focal point of the collector lens; an epi-illumination projection lens for projecting light source images generated by the fly-eye lens onto the objective pupil; a tube lens for imaging an observation light from the objective; arid a fluorescence cube placed between the objective arid projection lens and comprised a filter, satisfies the expression γ | 11-19-2009 |
| 20090296207 | Laser scanning microscope and its operating method - Laser scanning microscope and its operating method in which at least two first and second light distributions activated independently of each other and that can move in at least one direction illuminate a sample with the help of a beam-combining element, and the light is detected by the sample as it comes in, characterized by the fact that the scanning fields created by the light distributions on the sample are made to overlap mutually such that a reference pattern is created on the sample with one of the light distributions, which is then captured and used to create the overlap with the help of the second light distribution (correction values are determined) and/or a reference pattern arranged in the sample plane or in an intermediate image plane is captured by both scanning fields and used to create the overlap (correction values are determined) and/or structural characteristics of the sample are captured by the two scanning fields as reference pattern and used to create the overlap in which correction values are determined. | 12-03-2009 |
| 20090296208 | Observation device and wavelength limiting filter - An observation device observing a sample cultured in a culture vessel includes an illuminating unit including an illumination optical system and illuminating the sample, an image-capturing unit including an imaging sensor and generating an image by capturing an image of the sample illuminated by the illuminating unit, and a wavelength limiting filter being placed on an optical axis of the illumination optical system and between the illuminating unit and the image-capturing unit, and limiting a part of wavelengths of an illumination light from the illumination optical system in accordance with optical absorption properties of an additive contained in a culture medium used for culturing the sample. Accordingly, it is possible to suppress a change of the image resulting from the additive and to enable to generate an appropriate image in an automatic observation. | 12-03-2009 |
| 20090296209 | MICROSCOPE - A microscope includes a laser light source, an optical system which changes a beam diameter, and a field stop disposed at a position conjugating with a sample plane, in this order beginning from the side of the laser light source. In this microscopes the following conditional equation is satisfied: A≦D/2 where “A” is the diameter of the field stop, and “D” is the diameter of light incident to the field stop. | 12-03-2009 |
| 20090303583 | Device and method for the reproducible adjustment of the pin hole opening and pin hole position in laser scanning microscopes - Device to adjust the position and/or size of a pinhole in a laser scanning microscope (LSM) where the pinhole is illuminated via a separate light source or the LSM laser and the pinhole is moved at a right angle to the optical axis until the receiver has the maximum intensity and the pinhole position is captured and saved together with the data attributed to the replaceable optical components. | 12-10-2009 |
| 20090303584 | METHOD FOR LASER SCANNING MICROSCOPY AND BEAM COMBINER - A method for laser scanning microscopy is characterized by the use of encapsulated fiber multiplexers from the telecommunications field for combining the beams of a plurality of lasers of different wavelengths and coupling them together into a laser scanning microscope and by corresponding beam combiners. Light-conducting guides to which different lasers can be coupled, preferably by light guides, are advantageously guided out of an encapsulated component. | 12-10-2009 |
| 20090316258 | DUAL EMISSION MICROSCOPE - There is provided a microscope device comprising: means for creating a collimated beam of light collected from a sample and comprising at least a first spectral range and a second spectral range, means for separating the collimated beam into a first beam containing a higher percentage of light of the first spectral range than light of the second spectral range and a second beam containing a lower percentage of light of the first spectral range than light of the second spectral range, means for reflecting the first beam, means for reflecting the second beam, means for combining the first beam and the second beam, a detector, and means for imaging the combined first and second beam onto the detector in order to create an image of the sample on the detector, wherein the means for reflecting the first beam and the means for reflecting the second beam are arranged in such a manner that the image created by the first beam and the image created by the second beam are shifted relative to each other on the detector, wherein the means for reflecting the first beam is adapted to invert handedness of the first beam, and wherein the means for reflecting the second beam is adapted to preserve handedness of the second beam. | 12-24-2009 |
| 20090316259 | OPTICAL INDICATOR FOR MICROSCOPIC LASER BEAM MANIPULATION - An objective assembly for use with a microscope is provided. The objective has an optical axis that permits an image beam to be emitted through the objective toward the eyepiece of a microscope. A mirror is positioned at an angle to the optical axis of the objective. A laser assembly is positioned on a first side of the mirror for directing a laser beam toward said mirror so that the energy is reflected off the mirror and through the objective in a direction that is substantially aligned with the optical axis of the objective. An indicator assembly including a source of light is positioned with the light incident on the other side of the mirror to reflect a beam of light in a direction opposite to the direction of the laser beam to provide an optical representation at the eyepiece of a microscope of the position of the laser beam being emitted by the objective. | 12-24-2009 |
| 20090323181 | SURGICAL MICROSCOPY SYSTEM AND IMAGING METHOD - A surgical microscopy system having an illumination system is provided. The illumination system comprises a xenon gas discharge lamp and a spectral filter which is optionally positionable into an illumination beam path of the surgical microscopy system and removable from the same. The spectral filter substantially exhibits, in a wavelength range between 400 nm and 700 nm, a transmission increasing from about 0.12 to 1 having a gradient between 0.025/nm and 0.0035/nm, in particular 0.00293/nm. Thus, the illumination system is enabled to provide light having two different spectral characteristics which is advantageous in particular for imaging structures in the human eye scattering to a different degree. | 12-31-2009 |
| 20100002291 | LIGHTING SYSTEM, METHOD OF LIGHTING, AND SCANNING OPTICAL MICROSCOPE - Light emitter excitation light ( | 01-07-2010 |
| 20100007946 | SUBSTAGE, STAGE AND MICROSCOPE EQUIPPED THEREWITH - Provided is a substage for a microscope. The substage is provided with a plurality of fixing members for selectively fixing a plurality of stages, on which a sample is placed, each of which is equipped with a fixing member. | 01-14-2010 |
| 20100014156 | MICROSCOPE - A microscope for observing a sample containing a substance having at least two excited quantum states includes a pump light source | 01-21-2010 |
| 20100014157 | MULTISPECTRAL LIGHTING APPARATUS - The invention is directed to a multispectral illumination device for a microscope or for a reader. According to the invention, the illumination device comprises at least three receptacle positions for lighting modules and at least one receptacle position for coupling modules, the mechanical devices for connecting the lighting modules or coupling modules at the receptacle positions to the illumination device being designed in such a way that the lighting modules or coupling modules can be easily changed. Further, the receptacle positions are arranged in such a way that, with suitable selection of the lighting modules and coupling modules, all individual spectra of the lighting modules in a total spectrum are available simultaneously at the output of the illumination device. | 01-21-2010 |
| 20100020392 | Confocal microscope device - The present application detects a state change of an object in an optical axis direction by a single frame scanning for each round. A confocal microscope apparatus includes a detecting unit separating incident light into a light from a vicinity of a collecting point on the sample and a light from a peripheral of the vicinity and detecting each of the lights, and an image generating unit generating an image of the sample by a light signal from the vicinity and a light signal from the peripheral, in which a distinction between a light signal from the vicinity and a light signal from the peripheral is calculated for each of a plurality of images generated in a number of times of scanning without changing a collecting location in an optical axis direction. | 01-28-2010 |
| 20100027110 | OBSERVABLE CENTRIFUGAL APPARATUS AND OBSERVATION APPARATUS - Disclosed is an observable centrifugal apparatus capable of checking in real time a state of a sample during reaction of separation or synthesization in the form of a stable and high-quality image at a high frame rate. An observable centrifugal apparatus A has a rotary disc | 02-04-2010 |
| 20100039701 | Light stimulus apparatus and observation apparatus - A proposition is to reduce a waiting time during a light stimulus observation. In order to achieve the proposition, a light stimulus apparatus is characterized in that it includes a light path controlling unit that controls an irradiating position of light for stimulus on a specimen, and a controlling unit that generates a selected position signal of a selected position and an executive instruction signal for irradiation of the light for stimulus onto the specimen in conjunction with a confirm operation regarding the selected position performed by a pointing device on an image of the specimen displayed on a displaying unit, controls the light path controlling unit based on the selected position signal, and controls a light source which emits the light for stimulus based on the executive instruction signal. | 02-18-2010 |
| 20100053743 | APPARATUS FOR REAL-TIME THREE-DIMENSIONAL LASER SCANNING MICROSCOPY, WITH DETECTION OF SINGLE- AND MULTI-PHOTON FLUORESCENCE AND OF HIGHER ORDER HARMONICS - Apparatus for real-time three-dimensional laser scanning microscopy, where single-photon fluorescence light, multi-photon fluorescence light, and higher order harmonics generated in the sample are detected. The excitation light is focused into the sample in a three-dimensional matrix of focal points. Real-time three-dimensional image acquisition is obtained by fast scanning in the xy plane only. | 03-04-2010 |
| 20100067102 | OPTICAL MICROSCOPE - The present invention provides an optical microscope capable of suppressing unnecessary response light as a background and detecting desired response light in nonlinear optical response process with a good S/N ratio. The optical microscope for collecting, on a sample | 03-18-2010 |
| 20100067103 | MICROSCOPE DEVICE - With a microscope device according to the invention, it is possible to acquire information on a deeper part of a living organism than that in the case of a conventional microscope device. The microscope device according to a first invention comprises an illumination optical system for irradiating an object with illumination rays in a line-like form, a detection optical system for receiving light rays generated by the illumination rays. The second invention relates to a microscope device wherein an objective lens of an illumination optical system, and an objective lens of a detection optical system are at respective positions. The third invention relates to a microscope device wherein a separation filter is structured such that a laser beam can pass through only a part (a region) thereof. The fourth invention relates to a microscope device wherein illumination rays having coherence are separated into two portions. | 03-18-2010 |
| 20100085636 | Optical System for a Confocal Microscope - An optical system for a confocal microscope comprising: an illumination pattern ( | 04-08-2010 |
| 20100097693 | Confocal microscope - A confocal microscope that can reduce the measurement time is provided. Linear bright lines are extracted from the light of a light source. White light emitted from the linear bright lines is chromatically dispersed into continuous wavelength components by a chromatic aberration lens, which then irradiate a sample on a stage via an objective lens. The chromatically dispersed linear bright lines are continuously imaged for each wavelength on an optical axis in the height direction, and light having one wavelength is focused on one certain point on the surface of the sample. The reflected light of light having wavelengths focused on a surface of the sample is collected by the chromatic aberration lens via the objective lens and then focused on a slit. The light passed through the slit is separated by a spectroscopic device and imaged on a two-dimensional array photodetector. | 04-22-2010 |
| 20100097694 | METHOD AND ARRANGEMENT FOR CONTROLLED ACTUATION OF A MICROSCOPE, IN PARTICULAR OF A LASER SCANNING MICROSCOPE - Method for actuation control of a microscope, in particular of a Laser Scanning Microscope, in which, at least one first illumination light, preferably moving at least in one direction, as well as at least one second illumination light moving at least in one direction, illuminate a sample through a beam combiner, a detection of the light coming from the sample takes place, whereby, at least one part of the illumination light is generated through the splitting of the light from a common illuminating unit, characterized in that, by means of a common control unit, a controlled splitting into the first and the second illumination light takes place, in which the intensity of the first illuminating light, specified by the user or specified automatically, is assigned a higher priority (is prioritized) compared to the specified value for the second illumination light, and an adjustment for the second illumination light takes place until a maximum value is obtained, which is determined by the value specified for the first illumination light. | 04-22-2010 |
| 20100118394 | MICROSCOPE FOR CONVENTIONAL FLUORESCENCE MICROSCOPY AND TOTAL INTERNAL REFLECTION MICROSCOPY - A microscope for conventional fluorescence microscopy (epi-fluorescence) and for total internal reflection microscopy, comprising at least one light source ( | 05-13-2010 |
| 20100118395 | MODULAR OBJECTIVE ASSEMBLY - A modular laser objective for use with a microscope is provided. A mounting modular body permits the modular objective to be releasably mounted to the turret of a microscope. The objective has an optical axis that penults an image beam to be emitted through the objective toward the eyepiece of a microscope. The modular body supports a mirror positioned at an angle to the optical axis of the objective. A modular laser assembly is mounted on the modular body on a first side of the mirror for directing a laser beam toward the mirror so that the energy is reflected off the mirror and through the objective in a direction that is substantially aligned with the optical axis of the objective. A modular indicator assembly is received in the modular body and includes a source of light positioned with the light incident on the other side of the mirror to reflect a beam of light in a direction opposite to the direction of the laser beam to provide an optical representation at the eyepiece of a microscope or on a camera of the position on the target of the laser beam being emitted by the objective. | 05-13-2010 |
| 20100118396 | CONFOCAL MICROSCOPE APPARATUS - A microscope apparatus includes a first optical system which illuminates a sample via an objective lens with light output from a light source and which detects fluorescence emitted from the sample via the objective lens, and a second optical scanning system which irradiates specific regions of the sample with a laser beam output from a laser light source, thereby causing a particular phenomenon. The first optical system may include a rotatable disk to obtain a confocal effect, and the light output from the light source scans the sample via the rotatable disk, and the fluorescence is detected via the rotatable disk. A depth position of a focal plane of the second optical scanning system is generally the same as a depth position of a focal plane of the first optical system. | 05-13-2010 |
| 20100142040 | MICROSCOPE - A microscope with which a transparent or semitransparent sample can be stereoscopically observed is provided. The microscope includes a placement portion for placing the sample thereon, a magnifier for magnifying the sample to a size viewable through an objective lens, a pattern body where a pattern has been drawn, and a light projector for applying light to at least the pattern body. The placement portion, the pattern body, and the light projector are arranged at positions which enable light projected from the light projector to be applied through the pattern body to the sample arranged within a depth of field of the magnifier by the placement portion. | 06-10-2010 |
| 20100142041 | Multi-Mode Fiber Optically Coupling a Radiation Source Module to a Multi-Focal Confocal Microscope - A multi-mode optical fiber delivers light from a radiation source to a multi-focal confocal microscope with reasonable efficiency. A core diameter of the multi-mode fiber is selected such that an etendue of light emitted from the fiber is not substantially greater than a total etendue of light passing through a plurality of pinholes in a pinhole array of the multi-focal confocal microscope. The core diameter may be selected taking into account a specific optical geometry of the multi-focal confocal microscope, including pinhole diameter and focal lengths of relevant optical elements. For coherent radiation sources, phase randomization may be included. A multi-mode fiber enables the use of a variety of radiation sources and wavelengths in a multi-focal confocal microscope, since the coupling of the radiation source to the multi-mode fiber is less sensitive to mechanical and temperature influences than coupling the radiation source to a single mode fiber. | 06-10-2010 |
| 20100157422 | MICROSCOPE DEVICE - A microscope device including a coherent light source, an illuminating optical system which has light beam splitting means that splits the coherent light source into light beams and phase-modulating means disposed near a pupil conjugate plane and adapted for modulating the phases of two of the light beams and projects illuminating light spatially modulated into an interference fringe structure by causing the two light beams to interfere with each other near the plane of a sample, an imaging optical system for forming an image of the sample with diffracted light, imaging means, and image processing means for creating a sample image by computation of the image captured by the imaging means each time the phase of the spatial modulation is modulated. | 06-24-2010 |
| 20100182680 | ILLUMINATION OPTICAL SYSTEM FOR MICROSCOPE, AND MICROSCOPE - An illumination optical system includes, in order from a light source side a collector lens, a field stop, a field lens having positive power, an aperture stop, and a collective lens having positive power. The illumination optical system is a substantially both-side telecentric optical system between the field stop and a sample surface, and satisfies the following conditional expressions where D | 07-22-2010 |
| 20100182681 | LIGHT TRAP, COUPLING DEVICE FOR A BEAM PATH, AS WELL AS ILLUMINATION DEVICE AND OPTICAL OBSERVATION DEVICE - First of all, the present invention relates to a light trap ( | 07-22-2010 |
| 20100188741 | LASER SCANNING MICROSCOPE - A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity. | 07-29-2010 |
| 20100188742 | SLIT-SCAN MULTI-WAVELENGTH CONFOCAL LENS MODULE AND SLIT-SCAN MICROSCOPIC SYSTEM AND METHOD USING THE SAME - The present invention provides a slit-scan multi-wavelength confocal lens module, which utilizes at least two lenses having chromatic aberration for splitting a broadband light into continuously linear spectral lights having different focal length respectively. The present invention utilizes the confocal lens module employing slit-scan confocal principle and chromatic dispersion techniques and the confocal microscopy with optical sectioning ability and high resolution in spectral dispersion to establish a confocal microscopy method and system with long DOF and high resolution, capable of modulating a broadband light to produce the axial chromatic dispersion and focus on different depths toward an object's surface for obtaining the reflected light spectrum from the surface. Thereafter, the spectrum is spatially filtered by a slit and then a peak position with respect to the filtered spectrum along the scanning line is detected by a spectral image sensing unit for generating the sectional profile of the measured surface. | 07-29-2010 |
| 20100195198 | MICROSCOPE ILLUMINATION - The invention relates to a microscope illumination comprising at least one laser light source that emits a light beam, beam-guiding optical elements for generating an illumination beam path with distinguished planes, such as pupil and field planes, and a homogenizing arrangement for forming a luminous field that is homogenized with respect to the intensity and is to be directed onto a sample to be observed. | 08-05-2010 |
| 20100202043 | SLIT-SCANNING CONFOCAL MICROSCOPE - Slit-scanning confocal microscopes are disclosed having a slit-like light source, an illumination optical system, and an imaging optical system. The illumination optical system forms an image of the light source on a sample. The imaging optical system forms an image of the illuminated sample on a line sensor. The line sensor is situated optically conjugate to the light source and receives light from the sample as reflected light, transmitted light, or fluorescence light. The slit-like light source is divided into unit light sources each having a size that is optically conjugate to a respective pixel of the line sensor. | 08-12-2010 |
| 20100208339 | MICROSCOPE AND METHOD FOR OPERATING A MICROSCOPE - The invention relates to a method for operating a microscope in which excitation light is focused on, or beamed to, different points of a specimen, in which an intensity of the excitation light is point-specifically varied and in which an intensity of the light reflected by said specimen in at least one spectral range is measured point-specifically and quantitatively. The method according to the invention is characterized in that the intensity and/or a spectral composition of the excitation light beamed to a specific point of said specimen is automatically adjusted by a regulating device on the basis of information previously gained from measured data of said specimen concerning an estimated or actual intensity of the light reflected in the spectral range by said point such that an integral of the intensity of the light reflected in the spectral range by this point during a pixel dwell time is within a predefined value interval. The invention also relates to a microscope. | 08-19-2010 |
| 20100208340 | OBSERVATION DEVICE - An observation device that limits the brightness of an area to be a background of a sample, so that contrast of the sample can be clearly observed in the reflection observation method. An illumination light quantity limiting stop that is disposed on the emission-side pupil surface of the illumination light has a doughnut shape, by which an opening portion is formed at the center of a disk, so that the peripheral portion of the luminous flux of the illumination light is interrupted and only the center portion thereof is transmitted. An incident light quantity limiting stop that is disposed on the incident-side pupil surface has a circular shape smaller than the cross-section of the luminous flux, so that the center portion of the luminous flux is interrupted, and therefore only the peripheral portion of the luminous flux of the reflected light is transmitted. | 08-19-2010 |
| 20100214654 | APPARATUS FOR THE DETECTION OF LIGHT IN A SCANNING MICROSCOPE - A light detector for use in a line scanning microscope and a microscope comprising such a light detector are described. The light detector comprises—a line array of avalanche semiconductor detectors; and an electronic trigger circuit that is adapted to operate the avalanche semiconductor detectors in at least one of a Geiger mode with internal charge amplification and in a linear mode. The trigger circuit further comprises a parallel counter that is designed to read out in parallel light pulses detected by the avalanche semiconductor detectors. The parallel counter is adapted to accumulate the light pulses detected by the avalanche semiconductor detectors over a preset counting time. | 08-26-2010 |
| 20100245993 | LASER SCANNING MICROSCOPE - The present invention relates to a laser scanning microscope that can generate an arbitrary control signal without depending on the configuration of the hardware. A memory | 09-30-2010 |
| 20100245994 | SCANNING LASER MICROSCOPE - A scanning laser microscope obtains images by performing scanning with laser light. The scanning laser microscope includes scanner and controller. The scanner is applied for performing scanning with the laser light line by line. The controller is applied for calculating a required line scanning time that is required as time used for scanning one line, based on the time required for scanning one frame that is determined from a frame rate being set. The controller is also applied for adjusting at least one of a number of data in one line of drive waveform data for driving the scanner and a read cycle of the drive waveform data, so as to substantially match the time for the scanner to scan one line with the required line scanning time calculated by the controller. | 09-30-2010 |
| 20100254000 | MIRROR CASCADE FOR BUNDLING A PLURALITY OF LIGHT SOURCES AND A LASER-SCANNING MICROSCOPE - A mirror cascade for the adjustment-free bundling of a plurality of light sources to be coupled into the beam path of a laser scanning microscope, comprising a beam combiner housing in which the mirror cascade is located, wherein the beam combiner housing can be either mounted directly on a scanning head of a laser scanning microscope and has a direct optical connection thereto or can be mounted on a microscope housing and has an optical connection thereto or is directly arranged in the scanning head. The invention further relates to a laser scanning microscope with such a mirror cascade. | 10-07-2010 |
| 20100259816 | Illumination device - An illumination device for an object to be examined through the objective of a microscope, comprises an illumination housing to be releasably connectable to a microscope. The housing extends along an axis and has two open front sides for the path of rays of the microscope's objective. For illumination, there is at least one light source which exhibits a plurality of light exits arranged in the illumination housing; wherein the light exits are inclined with respect to the axis of the housing. A translucent plate is connected to and removable from the illumination housing in a tool-free releasable manner so as to cover one of the open front sides as well as the light exits and to serve as a support surface for the object in the cases of dark-field illumination and back lighting bright field illumination. | 10-14-2010 |
| 20100265574 | IMMERSION MICROSCOPE OBJECTIVE AND MICROSCOPE WITH IT - An immersion microscope objective comprises, in order from an object side, a first lens group having a positive refractive power comprising a cemented lens composed of a piano-convex lens whose plane surface faces the object side and a meniscus lens whose concave surface faces the object side, and at least one single lens having a positive refractive power; a second lens group having a positive refractive power comprising a three-piece cemented lens; and a third lens group having a negative refractive power including a Gaussian type lens structure, wherein the objective satisfies the following conditions when n | 10-21-2010 |
| 20100265575 | MICROSCOPE - A microscope including an imaging objective for imaging a sample on a detector and means for illuminating the sample with a light sheet in the focus plane of the imaging objective. The illumination means includes an illumination source which emits coherent light, and Bessel optics which generate at least two plane waves from the light beam and give propagation directions for the plane waves. The propagation direction of each of the plane waves encloses an acute angle with the focus plane in each instance, the magnitude of the acute angle being identical for each of the plane waves, so that the plane waves undergo constructive interference in the focus plane so that a light sheet is generated. Similarly, the illumination means can also include an optical element by which a rotationally symmetric Bessel beam is generated from the light beam for dynamic generation of a light sheet. | 10-21-2010 |
| 20100271695 | ARRANGEMENT FOR ANALYZING MICROSCOPIC AND MACROSCOPIC PREPARATIONS - A confocal scanning microscope for examining microscopic and macroscopic objects is described. The microscope comprises: a scanning optical system having optical elements imaging the light generated by a laser onto an object to be examined; an objective provided in a working distance of at least 0.4 inches from an object holder; and a zoom optical system that is connected to the scanning optical system such that the light generated by the laser passes first through the scanning optical system, then through the zoom optical system, and is then imaged through the objective onto the object. This microscope achieves that also macroscopic objects can be viewed at a high resolution. | 10-28-2010 |
| 20100271696 | ILLUMINATION APPARATUS AND MICROSCOPE HAVING THE SAME - An illumination apparatus includes a light source and a collimator optical system converting light emitted from the light source to approximately-parallel light. The collimator optical system includes, in an order of proximity to the light source, a first lens having a positive power and including an approximately-flat surface and an aspheric surface, and a second lens having a positive power. | 10-28-2010 |
| 20100290110 | MULTI-FUNCTION MICROSCOPE DEVICE - A multi-function microscope device comprises a main body and a light adjustment base; the main body having at least one light-emitting element, a magnetic element, and a transmitting element; a microscope lens being formed by a transparent element, a lens, and a magnetic element; a microscope device and being assembled by the lens, the main body, and the light adjustment base through the magnetic elements; a focusing element being formed by a focusing element retainer, a focus adjusting element, a cover retainer, and a cover; wherein the multi-function microscope device assembled by the microscope device and the focusing element can be arranged to a machine having a microscope equipment or a microscope frame, to be conveniently carried and used by a user as well as lowering the cost. | 11-18-2010 |
| 20100302629 | Surgical microscope having an illuminating system and control unit therefor - A surgical microscope ( | 12-02-2010 |
| 20100302630 | INCIDENT ILLUMINATION DEVICE FOR A MICROSCOPE - An incident illumination device for a microscope for providing oblique or straight incident illumination is described. The illumination device comprises a light source that includes at least two 2-dimensional, surface light-emitting segments and is imaged into an aperture plane of the incident illumination device. At least one of the at least two light-emitting segments of the light source is designed to be activated individually. Further, a microscope comprising this incident illumination device is described, and methods of using this microscope both for oblique and straight illumination are described. In addition to conventional incident bright-field illumination, the described microscope and methods of use thereof allow also to select between angular or oblique incident illumination. | 12-02-2010 |
| 20100302631 | MICROSCOPE - The invention is based on a microscope, in particular a fluorescence analysis microscope, comprising an illumination carrier and illumination units arranged thereon for a reflected-light illumination of a sample region. In order to be able to illuminate a sample region uniformly in a simple manner by means of a compact device, it is proposed that at least three illumination units for the simultaneous reflected-light illumination of the sample region from different directions are arranged on the illumination carrier. | 12-02-2010 |
| 20100309548 | METHOD AND OPTICAL ASSEMBLY FOR ANALYSING A SAMPLE - A method and an arrangement for analyzing a specimen, wherein the specimen is supported so as to be rotatable around an axis of rotation and displaceable in all three spatial directions and is illuminated by a first illumination device. Light radiated from the specimen is imaged on a detection device. A plurality of sectional images of the specimen are recorded at different settings of the rotational angle, and the specimen is rotated. The recorded sectional images are fused to form a data set of spatial image data of the specimen. The specimen is then illuminated by a second illumination device perpendicular to the axis of rotation, wherein a plurality of shadow images of the specimen are recorded and the specimen is rotated. A second data set of spatial image data of the specimen is constructed from the recorded shadow images by means of a back projection algorithm. | 12-09-2010 |
| 20100309549 | LIGHT SOURCE ARRANGEMENT FOR AN ILLUMINATION DEVICE OF A MEDICAL-OPTICAL OBSERVATION - A light source arrangement ( | 12-09-2010 |
| 20100321772 | Illuminating system and an optical viewing apparatus incorporating said illuminating system - An illuminating system ( | 12-23-2010 |
| 20100328765 | ILLUMINATION OPTICAL SYSTEM AND FLUORESCENT MICROSCOPE - An illumination optical system which irradiates a sample surface with light through an illuminating lens includes: a light source; a condensing optical system receiving the light emitted from the light source; and a lens array optical system including a first lens array surface and a second lens array surface each formed by a plurality of lens elements. The first lens array surface has a conjugate relation with a back focal position of the illuminating lens. The second lens array surface is placed at a back focal position of the lens array optical system, has a conjugate relation with a pupil position of the illuminating lens, and the light source and the condensing optical system are arranged to form an image of the light source on the first lens array surface. | 12-30-2010 |
| 20110002033 | MICROSCOPE SYSTEM AND METHOD FOR CONTROLLING IT - A microscope system includes: an illumination unit; a light control direction unit specifying quantity of light output from the illumination unit; a storage unit storing voltage-related information in which a direction value specified by the light control direction unit and a voltage value to be applied to the illumination unit are associated with each other and are set, the direction value being in an entire range that can be specified by the light control direction unit under each observation condition, the voltage value corresponding to the direction value; a light quantity control unit controlling the quantity of the emitted light; and a control unit acquiring the voltage value corresponding to the specified direction value from the voltage-related information corresponding to the observation condition, and allowing the light quantity control unit to control the quantity of light based on the acquired voltage value. | 01-06-2011 |
| 20110013274 | EXTREME ULTRAVIOLET MICROSCOPE - An extreme ultraviolet (EUV) microscope configured to analyze a sample. The microscope includes a source of EUV radiation constructed and arranged to generate the EUV radiation with a wavelength at least in a range of about 2-6 nm, and an optical system constructed and arranged to illuminate the sample with the EUV radiation and to collect a radiation emanating from the sample. The optical system is arranged with at least one mirror that includes a multilayer structure for in-phase reflection of at least a portion of the radiation in the range of about 2-6 nm. | 01-20-2011 |
| 20110043906 | IMMERSION MICROSCOPE OBJECTIVE AND LASER SCANNING MICROSCOPE SYSTEM USING SAME - An immersion microscope objective formed of thirteen or fewer lens elements includes, in order from the object side, first and second lens groups of positive refractive power, a third lens group, a fourth lens group having negative refractive power with its image-side surface being concave, and a fifth lens group having positive refractive power with its object-side surface being concave. The first lens group includes, in order from the object side, a lens component that consists of a lens element of positive refractive power (when computed as being in air) and a meniscus lens element having its concave surface on the object side. Various conditions are satisfied to ensure that images of fluorescence, obtained when the immersion microscope objective is used in a laser scanning microscope that employs multiphoton excitation to observe a specimen, are bright and of high resolution. Various laser scanning microscopes are also disclosed. | 02-24-2011 |
| 20110051233 | SCANNING CONFOCAL MICROSCOPY - Known scanning confocal microscope systems use an optical fibre to deliver light to the confocal scanning head. The illumination from the fibre may be uneven and significant light loss may occur in the fibre. According to the invention, an assembly is provided for inputting a light beam from a light source into the confocal scanning head of a scanning confocal microscope system, wherein the assembly comprises a beam width adjuster ( | 03-03-2011 |
| 20110051234 | LASER SCANNING MICROSCOPE HAVING VARIABLE LIGHT INTENSITY AND CONTROL METHOD FOR THE SAME - A laser scanning microscope (LSM) having variable light intensity and a control method for the same. The light intensity of a laser beam in an LSM has been controlled to date with high accuracy, but also high costs by means of an acousto-optic component (AOM, AOTF). According to the invention, such a component for beam modulation is to be omitted, without reducing the exposure accuracy of the sample. In an LSM, a directly modulated laser diode ( | 03-03-2011 |
| 20110058253 | MICROSCOPE AND METHOD FOR CONTROLLING MICROSCOPE - A microscope includes a microscope main unit including a stage on which a specimen is to be placed; and a light source provided on the microscope main unit and emitting illumination light for illuminating the specimen. The microscope also includes a main power supply operable to be turned on and off; a sensor that senses the presence or absence of a subject in a front or side area of the microscope main unit; a determining unit that determines based on a result of the sensing whether the subject moves; and a control unit that turns on the light source when the main power supply is turned on, maintains the light source in an ON state when the determining unit determines that the subject moves, and turns off the light source when the determining unit determines that the subject does not move and a predetermined period of time passes. | 03-10-2011 |
| 20110075255 | Low Numerical Aperture Exclusion Imaging - In accordance with one embodiment of the present invention an apparatus for a low numerical aperture exclusion imaging apparatus is provided. The apparatus may include an electromagnetic illumination source for illuminating a portion of a specimen, and for collecting an image created by the electromagnetic radiation an objective lens optically coupled to the electromagnetic illuminated portion of the specimen. The apparatus also includes an optical blocking plate disposed between the objective lens and a focusing lens. The optical blocking plate is positioned to substantially block undesired electromagnetic radiation from image sources distally aligned in the same optical axis as the specimen. This invention enhances narrow depth of field characteristics in imaging. It also enhances discreet imaging in a narrow focus field by eliminating some of most of the light which contributes to wide depth of field focus. This is useful for optical sectioning ranging from microscopy to photography. | 03-31-2011 |
| 20110080638 | Microscope - A microscope includes a microscope basis for placing the microscope onto a surface, e.g. of a working table, a microscope pillar, which stands on the basis and extends substantially in upright direction, a support arm on the pillar, which projects from it and can be adjusted in height from the basis and supports an optical or opto-electrical observing system. The support arm has substantially light-tight walls, which surround at least one cavity. This cavity is located between the optical system and a region that is at least near the microscope pillar. In at least one embodiment, the at least one component is located within the cavity and includes an internal light source. There is a plug-in and holding system for the energy guide, which is either light energy and/or electrical energy. | 04-07-2011 |
| 20110090561 | MICROSCOPE - In microscopes, particularly laser scanning microscopes, for detecting light coming from a sample, it is known to protect detectors from excessively high light outputs by means of shutters in the detection beam path. Further, in order to measure the light output impinging on the detector when the detection beam path is closed, a portion of the light is coupled out of the detection beam path and directed to a monitor diode. Constructions of this kind are complicated and costly. In the microscope according to the invention, a monitor diode is arranged on the shutter in such a way that the monitor diode is situated in the detection beam path when the shutter is closed. This makes it possible in a simple manner to measure the light output in a microscope when the detection beam path is closed even without additionally coupling light out of the detection beam path. | 04-21-2011 |
| 20110102888 | MICROSCOPE - Provided is a microscope that allows irradiation with uniform illumination light without decreasing the amount of light. Employed is a microscope | 05-05-2011 |
| 20110109961 | PATTERN PROJECTION APPARATUS, SCANNING CONFOCAL MICROSCOPE, AND PATTERN RADIATING METHOD - A pattern projection apparatus includes: a spatial light modulator having a plurality of pixel devices each independently modulating light, and arranged at an optically conjugate position with respect to a sample; and a control device for dividing a modulation pattern of the spatial light modulator for irradiating the sample with illuminating light of a target form into a plurality of submodulation patterns and controlling the spatial light modulator sequentially for each of the plurality of submodulation patterns. | 05-12-2011 |
| 20110109962 | OPTICAL PHASE CONJUGATION 4 PI MICROSCOPE - A 4-Pi microscope for imaging a sample, comprising a first objective for focusing a first light beam on the sample at a spatial point one or more Digital Optical Phase Conjugation (DOPC) devices, wherein the DOPC devices include a sensor for detecting the first light beam that has been transmitted through the sample and inputted on the sensor; and a spatial light modulator (SLM) for outputting, in response to the first light beam detected by the sensor, a second light beam that is an optical phase conjugate of the first light beam; and a second objective positioned to transmit the first light beam to the sensor and focus the second light beam on the sample at the spatial point, so that the first light beam and the second light beam are counter-propagating and both focused to the spatial point. | 05-12-2011 |
| 20110116163 | SYSTEM FOR THE STRUCTURED ILLUMINATION OF A SAMPLE - The invention relates to a structured illumination system ( | 05-19-2011 |
| 20110116164 | MICROSCOPE AND MICROSCOPE LENS BARREL - A microscope includes a fixed section, a moving section that is movable with respect to the fixed section, and a bellows type expandable light shielding section provided between the fixed section and the moving section, wherein bending sections of the light shielding member are thicker than wall sections of the light shielding member. | 05-19-2011 |
| 20110122488 | MULTIPLE-PHOTON EXCITATION LIGHT SHEET ILLUMINATION MICROSCOPE - An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal. illumination of light sheet microscopy are provided. With live imaging of whole | 05-26-2011 |
| 20110122489 | MICROSCOPE APPARATUS - An arbitrary entire region of a specimen, or a plurality of individual regions, is simultaneously stimulated without a time lag, or a strong stimulus is applied to an arbitrary region of a specimen. The invention provides a microscope apparatus including a first stimulation optical system having a galvanometer mirror that scans a specimen with first stimulus light, which applies an optical stimulus to a specimen, on the specimen; a second stimulation optical system which has a plurality of two-dimensionally arrayed movable mirrors and which switches the angle of each movable mirror so that second stimulus light, which applies an optical stimulus to the specimen, can be selectively deflected towards the specimen; and a dichroic mirror that combines a light path of the first stimulation optical system and a light path of the second stimulation optical system. | 05-26-2011 |
| 20110122490 | SURGICAL MICROSCOPE | 05-26-2011 |
| 20110134519 | Imaging Distal End of Multimode Fiber - Where a multimode fiber is used for light delivery in a microscope system and a transverse distribution of light exiting a distal end of the fiber is substantially uniform, the distal end is imaged onto a plane of a sample to be probed by the microscope system, or at a conjugate plane. Alternatively, the distal end is imaged onto a plane sufficiently close to the sample plane or the conjugate plane such that a radiant intensity of light at the sample plane or the conjugate plane is substantially uniform. In the case of a multi-focal confocal microscope system, the distal end of the multimode fiber is imaged onto a plane of a segmented focusing array. Alternatively the distal end is imaged onto a plane sufficiently close to the segmented focusing array plane such that a radiant intensity of the light at the segmented focusing array plane is substantially uniform. | 06-09-2011 |
| 20110141557 | Laser scan confocal microscope - Fluorescence is generated from an irradiated point on an inspection surface of a sample and the fluorescence is collected by an objective lens. Here, because of the magnification chromatic aberration of the objective lens | 06-16-2011 |
| 20110141558 | SPHERICAL ABERRATION CORRECTION FOR NON-DESCANNED APPLICATIONS - Spherical aberration is the primary cause of lose of signal while imaging deeper into a sample. Spherical aberration is corrected in the imaging path of a non-descanned detection system (such as a multi-photon microscope). This corrects the illumination spot for artifacts caused by imaging deep into a sample. One exemplary advantage to this instrument is that it allows deeper and brighter imaging. | 06-16-2011 |
| 20110149388 | OPERATING CIRCUIT AND CONTROL METHOD FOR A PHOTOMULTIPLIER - A operating circuit and control method for protecting a PMT having a photocathode, a plurality of dynodes and an anode against overloading with a shorter reaction time, and to allow it to be switched on again rapidly. For this purpose, a switch is provided for electrically short circuiting the photocathode with the first dynode, or a switch is provided for reversing the polarity of the voltage between the photocathode and the first dynode. | 06-23-2011 |
| 20110164312 | IMMERSION CONTACT MODULE FOR BINOCULAR OPTICAL SYSTEMS WITH ANGULAR LIGHT SHUTTER DEVICE - The CONTACT MODULE ( | 07-07-2011 |
| 20110170180 | ELECTROSTATIC DEFORMABLE MIRROR USING UNITARY MEMBRANE - A deformable mirror for an adaptive optical system employs a thin membrane stretched over a plurality of electrostatic electrodes providing local controlled deformation to the membrane. | 07-14-2011 |
| 20110170181 | MICROSCOPE AND LAMPHOUSE - A microscope includes an illuminating unit that includes an excitation light source emitting an excitation light, and a phosphor receiving the excitation light and emitting illumination light in a specific wavelength range. The illuminating unit illuminates a specimen with the illumination light. The microscope also includes an observation unit for observing the specimen illuminated by the illuminating unit. | 07-14-2011 |
| 20110181947 | MICROSCOPE INSPECTION DEVICE FOR FLUORESCENCE INSPECTIONS - A microscope inspection device for performing a fluorescence inspection of a testing object includes a camera module, a microscope lens, a light source module, and a first light filter. The camera module includes an image sensor for sensing an image light of the testing object. The microscope lens has an end connected to the camera module and the microscope lens is used for amplifying the image light of the testing object. The light source module is connected to the microscope lens and emits a light of a first wavelength towards the testing object. The first light filter is installed at another end of the microscope lens and provided for allowing a light of a second wavelength only to pass through the microscope lens. | 07-28-2011 |
| 20110194174 | OPTICAL SYSTEM FOR CELL IMAGING - A microscope system ( | 08-11-2011 |
| 20110199676 | Confocal microscope - A confocal microscope ( | 08-18-2011 |
| 20110211254 | LASER SCANNING MICROSCOPE FOR SCANNING ALONG A 3D TRAJECTORY - The invention relates to a laser scanning microscope ( | 09-01-2011 |
| 20110216404 | CONFOCAL MICROSCOPE SYSTEM - There is provided a confocal microscope system configured so as to be compact in size without the needs for a large space, requiring fewer spots for adjustment. In the confocal microscope system, respective units making up the confocal microscope system are integrally housed in a protection cabinet covering the confocal microscope system, and when a specimen disposed opposite to an objective lens is moved toward an external face of the protection cabinet, a side of the external face, adjacent to an opening through which the specimen is taken in, or out, is defined as a front face, the Nipkow disk type scanner unit is disposed backward of the objective lens. | 09-08-2011 |
| 20110235170 | INCIDENT-LIGHT FLUORESCENT ILLUMINATION DEVICE AND FLUORESCENT MICROSCOPE USING THE DEVICE - An incident-light fluorescent illumination device includes: a light source emitting illumination light; a collector lens receiving the illumination light from the light source; a fly-eye lens optical system receiving the illumination light from the collector lens; an objective emitting the illumination light to a sample surface; and a relay optical system arranged between the fly-eye lens optical system and the objective. The fly-eye lens optical system includes a first fly-eye lens surface and a second fly-eye lens surface each having a plurality of lens elements. The incident-light fluorescent illumination device satisfies the conditional expression of | 09-29-2011 |
| 20110235171 | MICROSCOPE ADAPTER UNIT - A microscope adapter unit disposed on an optical path of illumination light between a light source unit including a light source and a sample surface includes a first lens group having at least one lens and a second lens group having at least one lens. The first lens group converts the illumination light into roughly parallel luminous fluxes, and makes the illumination light enter the second lens group. | 09-29-2011 |
| 20110255157 | Microscope apparatus - Illuminating light is two-dimensionally scanned without changing the ability to focus illuminating light on a specimen. A microscope has a spatial light modulator for the wavefront of illuminating light from a light source; a scanner having two mirrors independently pivoted about two non-parallel axes; a relay optical system guiding the illuminating light, whose traveling direction has been changed by the scanner, to an objective optical system; and an adjusting unit that moves a wavefront modulation region of the modulator, in which an image is formed, in response to pivoting of the mirrors, such that an image at the pupil position of the objective optical system assuming that the mirrors are stopped is moved opposite to the direction of movement of the image relayed to the pupil position of the objective optical system assuming that the mirrors are pivoting while the image on the spatial light modulator is fixed. | 10-20-2011 |
| 20110267688 | Microscopy Method and Microscope With Enhanced Resolution - Method for enhancing the resolution of a microscope during the detection of an illuminated specimen and a microscope for carrying out the method, wherein in a first position, an illumination pattern is generated on the specimen, the resolution of which is preferably within the range of the attainable optical resolution of the microscope or higher, wherein a relative movement, preferably perpendicular to the direction of illumination, from a first into at least one second position of the illumination pattern on the specimen is generated at least once between the detection and the illumination pattern with a step width smaller than the resolution limit of the microscope and detection and storage of the detection signals take place both in the first and in the second position. | 11-03-2011 |
| 20110299156 | METHOD FOR FCS MEASUREMENTS - A method for conducting FCS measurements includes providing a sample volume, emitting a target light having a first wavelength from a target light source, and marking an FCS volume in the sample volume with the target light by directing the target light onto the sample volume. An illuminating light having a second wavelength is emitted from an illuminating light source, the second wavelength being different than the first wavelength, and the illuminating light is directed onto the sample volume. | 12-08-2011 |
| 20120008196 | ATR objective for an IR microscope and method for operation thereof - ATR (attenuated total reflection) objective ( | 01-12-2012 |
| 20120062987 | ADAPTOR FOR MICROSCOPES - A fluorescent microscope attachment is disclosed that includes a removable filter arm to provide an adaptor for use in transforming a light microscope into a fluorescent microscope. The adaptor may further include an LED light source and/or a magnetic microscope objective attachment. | 03-15-2012 |
| 20120099190 | SPIM Microscope with a sequential light sheet - A SPIM-microscope (Selective Plane Imaging Microscopy) having a y-direction illumination light source and a z-direction detection light camera. An x-scanner generates a sequential light sheet by scanning the illumination light beam in the x-direction. By an illumination optics having a zoom optics that is provided in the beam path of the illumination light beam the focal length of the illumination light beam can be varied. | 04-26-2012 |
| 20120105949 | Additive Manufacturing-Based Compact Epifluorescence Microscope - An epifluorescence microscope achieves a compact form factor without sacrificing optical sensitivity by the novel use of combined optic mounts and light baffles constructed using additive manufacturing processes. The use of additive manufacturing enables stray-light-capturing structures that are not practical to make by other techniques. Some embodiments of the present invention do not require installation of filters by an operator, reducing the likelihood of dust and contamination on optical surfaces. Some embodiments of the present invention employ a novel light path that avoids passing the fluorescent light through off-axis elements. This optical arrangement provides for the use of a microscope objective having a finite corrected-image distance, such as a DIN objective, rather than infinity-corrected objective that require additional optical elements to form an image. The reduction in complexity can both reduce system cost and improve optical performance by reducing Fresnel losses and imaging artifacts from Fresnel reflections. | 05-03-2012 |
| 20120113506 | Novel Multi-Point Scan Architecture - The embodiments of this invention use a multi-point scanning geometry. This design maintains the high frame rate of the slit-scan system and still allows both grayscale and multi-spectral imaging. In a confocal configuration, the multi-point scanning system's confocal performance is close to that of a single point scan system and is expected to yield improved depth imaging when compared to a slit-scan system, faster imaging than a point scan system, and the capability for multi-spectral imaging not readily achievable in a Nipkow disk based confocal system. | 05-10-2012 |
| 20120127569 | MICROSCOPE APPARATUS - A microscope apparatus including an illumination apparatus capable of, regardless of the observation magnification of an imaging optical system, filling the entrance pupil of the imaging optical system with illumination light, and suitably restricting the conjugate image of the entrance pupil of the imaging optical system by a light shielding element. A microscope apparatus is configured by including: an illumination apparatus which includes a surface light emitter (light guide plate) having a light emitting surface as a planar light-emitting region and irradiates a sample with light emitted from the light guide plate; an objective lens which condenses light from the sample; and an imaging optical system which includes a variable power lens group configured to form an image of the sample by changing the magnification of the image of the sample. The illumination apparatus includes a light shielding plate and a light diffusing element. | 05-24-2012 |
| 20120140317 | METHOD FOR EVALUATING FLUORESCENCE RESULTS IN A MICROSCOPE IMAGE - The invention allows a quantitative evaluation of images acquired by microscope having fewer errors and is applicable in connection with high-resolution methods, particular at a high speed. A microscope image is analyzed in which the intensity distributions of fluorescence events have in each instance a diffraction-dependent extent which corresponds to an extent of a point spread function of the microscope and are arranged so as to be spatially non-overlapping, or at least predominantly spatially non-overlapping, in that at least one counter is initialized for every region to be analyzed in the microscope image, at least one fluorescence event is identified in a region to be analyzed in the microscope image, and the counter corresponding to the relevant region is incremented for each fluorescence event identified in the region. The counting results in a dramatic improvement in the signal-to-noise ratio at a high evaluation speed. | 06-07-2012 |
| 20120162754 | Pinhole for a Confocal Laser Scanning Microscope - A laser scanning microscope comprising having a light source, an illuminating beam for illuminating a specimen with a spatially limited, preferably point-shaped, illuminating light spot via a scanning device and an objective lens, and a detection beam for detecting the specimen light that reaches the detection beam via the objective lens and the scanning device. Focusing means that are disposed in the detection beam and focus the light from the specimen to form a spatially limited detection light spot in a plane. Receiver elements are distributed in the spot in a matrix-like manner and can be individually read out. These are located in order to simulate an adjustable pinhole aperture. | 06-28-2012 |
| 20120176672 | SYSTEMS FOR CHROMATIC ABERRATION CORRECTION IN TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY - Correction elements that can be incorporated in objective-based TIRF microscopy instruments to correct for chromatic aberrations are described. A correction element can be placed between a multiple wavelength excitation beam source and the microscope objective lens. In one aspect, the thickness of the correction element is defined to compensate for different axial positions of the focal points associated with each excitation wavelengths traveling along the outer edge of lenses comprising a microscope objective lens. In another aspect, the correction element can be angled and/or configured so that the different wavelengths of multiple wavelength excitation light are shifted to adjust the angle of incidence for each wavelength at the specimen/substrate interface. | 07-12-2012 |
| 20120206798 | LIGHT-PAD MICROSCOPE FOR HIGH-RESOLUTION 3D FLUORESCENCE IMAGING AND 2D FLUCTUATION SPECTROSCOPY - A microscope is described, having an illumination light path for illuminating a sample or object and a viewing light path for viewing the sample. The microscope includes an illumination light path focussing arrangement in the illumination light path, defining a substantially two-dimensional sample or object illumination region extending along an illumination direction of the illumination light path and transversely thereto. The microscope further includes an illumination region-confining device in the illumination light path for selectively illuminating a portion of the substantially two-dimensional object illumination region, wherein the portion of the substantially two-dimensional object illumination region is confined at least in the illumination direction and/or in the direction transversely thereto. | 08-16-2012 |
| 20120243080 | MICROSCOPE - A laser microscope that efficiently performs 3D imaging irrespective of the shape of a specimen, includes an area segmenting section that segments an observation range of a specimen in the direction perpendicular to the optical axis of an objective lens into many areas; a surface-position storing section that stores motorized stage positions in association with the specimen surface positions, for the many areas; a surface-shape estimating section that estimates the specimen surface shape from the motorized stage positions and the specimen surface positions for the many areas; a z-scanning condition determining section that determines the specimen surface positions at desired positions of the motorized stage from the specimen surface shape; and a light detecting section that detects light from the specimen over certain ranges specified with reference to the specimen surface positions, in the optical axis direction of the objective lens. | 09-27-2012 |
| 20120250149 | Ultrahigh-Wavenumber Transmitting Element and Near-Field Optical Microscope Using Thereof - An ultrahigh-wavenumber transmitting element has at least two anisotropic media having slopes of isofrequency curves complementary with each other. The at least two anisotropic media are layered so as to transmit ultrahigh wavenumber. | 10-04-2012 |
| 20120262782 | Multiple Wavelength LED Array Illuminator for Fluorescence Microscopy - One embodiment provides light along an optical axis. It comprises a substrate and at least one array of multiple LED chips without individual packaging supported by the substrate. The LED chips emit light within different wavelength ranges and are distributed laterally with respect to the axis over an area, the LED chips having light emitting surfaces for emitting light in directions transverse to the area. An optical element adjacent to the light emitting surfaces of the LED chips in the at least one array collects and directs light emitted by the LED chips of the at least one array along the axis towards a target. Another embodiment is directed to a method for providing multiple wavelength light for fluorescent microscopy using the above system. Electric current is supplied to the multiple LED chips, causing them to emit light of multiple wavelengths. The currents supplied to the multiple LED chips are controlled so as to control the exposure of fluorescent dyes with different excitation wavelengths wherein the light emitted by the multiple LED chips include wavelength components at such different excitation wavelengths without having to move the multiple LED chips. | 10-18-2012 |
| 20120275019 | Electronic Microscope Filter - The present invention discloses a unique and novel combination light source and active light filtering system for microscopes that eliminates the need for individual color filters, fluorescence filters, and many other filter types. The present invention provides variable light wavelength generating capabilities, and all of the benefits of most commercially available light sources in a compact package that can be mounted on a microscope or used at a distance from a microscope, but be coupled to it through a fiber optic cable or other light transmission means. Additionally, the present invention eliminates the need for a filter wheel turret in a microscope's optical path, as well as eliminates the need for multiple fluorescent filter blocks in a fluorescent microscope optical path. The present invention can improve microscope filter systems to enable effective imaging of live cells without staining. | 11-01-2012 |
| 20120293863 | FLUORESCENCE COLLECTION OBJECTIVE OPTICAL SYSTEM AND METHOD - An optical system particularly suited for non-linear fluorescence collecting includes a front lens system, a rear lens system, and a bulk, dichroic beam splitting component intermediate the front lens system and the rear lens system to direct the fluorescent emission from a target object to a photodetector. A lens housing may have a reflective coating on an interior surface thereof. The objective optical system is particularly advantageous for use in cases where large fields of view and high collection efficiencies are desirable. | 11-22-2012 |
| 20120300293 | Simple Ultra-Stable Stage with Built-in Fiduciary Markers for Fluorescence Nanoscopy - An improved microscope stage mount with built-in fiduciary markers is used for fluorescence microscopy, and comprises: (a) an optically-transparent glass plate adapted for specimen mounting and microscope viewing and comprising a specimen mounting area; and (b) a defined and ordered, two-dimensional microscopic array of fiduciary markers, wherein the markers are polymeric pillars affixed to the plate about the specimen mounting area, wherein the markers provide a three-dimensional spatial reference for the specimen. | 11-29-2012 |
| 20120300294 | FLUORESCENCE OBSERVATION SYSTEM AND SET OF FILTERS - A fluorescence observation system, a method for performing a fluorescence observation, and a set of filters that can be used in such system and method are provided. | 11-29-2012 |
| 20120300295 | LIGHT STIMULUS APPARATUS AND OBSERVING APPARATUS WITH LIGHT CONTROLLING UNIT - A proposition is to reduce a waiting time during a light stimulus observation. In order to achieve the proposition, a light stimulus apparatus is characterized in that it includes a light path controlling unit that controls an irradiating position of light for stimulus on a specimen, and a controlling unit that generates a selected position signal of a selected position and an executive instruction signal for irradiation of the light for stimulus onto the specimen in conjunction with a confirm operation regarding the selected position performed by a pointing device on an image of the specimen displayed on a displaying unit, controls the light path controlling unit based on the selected position signal, and controls a light source which emits the light for stimulus based on the executive instruction signal. | 11-29-2012 |
| 20120307354 | RETRACTABLE BEAM SPLITTER FOR MICROSCOPE - Systems and methods are provided for illuminating a surface to be observed microscopically using a retractable beamsplitter. The retractable beamsplitter allows the use of coaxial illumination when the beamsplitter is positioned in the operator's line of sight. The retractable beamsplitter allows the use of non-coaxial illumination without reducing the amount of illumination that reaches the operator when the beamsplitter is retracted from the operator's line of sight. As a result a single system can be used effectively to provide various types of illumination. | 12-06-2012 |
| 20120320454 | HEAD-WORN ILLUMINATORS AND MAGNIFIERS WITH OPTICAL REJECTION COATINGS TO ASSIST MEDICAL AND DENTAL PROFESSIONALS - An improved head-mounted optical illuminator or magnifier of the type worn by a medical or dental professional includes an optical coating applied to one or more optical surfaces associated with the illuminator or magnifier, and wherein the optical coating is a rejection coating operative to blocks wavelengths in the green, blue, violet and/or ultraviolet portions of the electromagnetic spectrum, depending upon the embodiment. Short-wavelength coatings (blue/violet/uv) may be applied to the surface of a lens used in a head-worn illuminator, for example to the beam-forming optics. The head-worn illuminator may be an LED illuminator, xenon illuminator, or other high-intensity source. In the case of the green notch filter coatings, these would typically only be applied to a head-worn magnifier, including flip-up and through-the-lens styles. In all embodiments, the optical coating may be a multilayer dielectric coating, a holographic filter, or utilize other optical filter technology. | 12-20-2012 |
| 20120327509 | MICROSCOPE - The present invention relates to a microscope of which visibility, controllability and operability can be improved. | 12-27-2012 |
| 20130003172 | SCANNING MICROSCOPE AND METHOD FOR OPTICALLY SCANNING ONE OR MORE SAMPLES - A device in the form of a scanning microscope, a device in the form of a structural unit for a microscope and a method and a device for optically scanning one or more samples. A device in the form of a scanning microscope has a light source ( | 01-03-2013 |
| 20130010353 | Multi-Mode Fiber Optically Coupling a Radiation Source Module to a Multi-Focal Confocal Microscope - A multi-mode optical fiber delivers light from a radiation source to a multi-focal confocal microscope with reasonable efficiency. A core diameter of the multi-mode fiber is selected such that an etendue of light emitted from the fiber is not substantially greater than a total etendue of light passing through a plurality of pinholes in a pinhole array of the multi-focal confocal microscope. The core diameter may be selected taking into account a specific optical geometry of the multi-focal confocal microscope, including pinhole diameter and focal lengths of relevant optical elements. For coherent radiation sources, phase randomization may be included. A multi-mode fiber enables the use of a variety of radiation sources and wavelengths in a multi-focal confocal microscope, since the coupling of the radiation source to the multi-mode fiber is less sensitive to mechanical and temperature influences than coupling the radiation source to a single mode fiber. | 01-10-2013 |
| 20130021663 | MICROSCOPE SYSTEM - A plurality of mounting devices are selectively exchanged while keeping the same parfocal distances for objective lenses among these mounting devices. A microscope system includes a microscope main unit and a plurality of attachable/detachable objective lens units that are selectively attached to the microscope main unit. The microscope main unit includes raising mechanism that can move the attached objective lens unit in an optical axis direction. The plurality of objective lens units have a revolver or a nosepiece that can be attached to the microscope main unit and an objective lens that can be mounted on the revolver or the nosepieces in an attachable/detachable manner. Distances from an attachment position in the microscope main unit for the revolver or the nosepiece to focal positions of the objective lenses are set to be mutually equal among the objective lens units. | 01-24-2013 |
| 20130027769 | Microscope Illumination Method and Microscope - The present invention relates to a method for illuminating a sample ( | 01-31-2013 |
| 20130044370 | DEVICE AND METHOD FOR SCANNING AN OBJECT AND A MICROSCOPE - The invention relates to a device for scanning an object comprising a focusing lens system ( | 02-21-2013 |
| 20130050813 | LOCALIZED SURFACE PLASMON RESONANCE BASED SUPER RESOLVED TOTAL INTERNAL REFLECTION FLUORESCENCE IMAGING APPARATUS, AND DETECTION MODULE THEREFOR - A total internal reflection fluorescence imaging apparatus according to an embodiment of the invention includes: a metal nanostructure layer, which includes a metal thin film and a nanostructure formed over the metal thin film; a light source unit, which provides incident light such that the incident light is totally reflected off the metal nanostructure layer and an evanescent wave localized in a horizontal direction is created between the metal nanostructure layer and a specimen arranged over the metal nanostructure layer; and a fluorescence image extracting unit, which extracts and images a fluorescence signal generated by the specimen due to the evanescent wave localized in a horizontal direction. | 02-28-2013 |
| 20130070335 | MICROSCOPE HAVING A TRANSMITTED-LIGHT ILLUMINATING DEVICE FOR CRITICAL ILLUMINATION - A microscope includes a light source including an LED device having a light radiating surface and a light directing element including a larger coupling-out surface. The light directing element is disposed so as to couple in light radiated by the light source and couple out the radiated light from the coupling-out surface. The light directing element is disposed so that the light is radiated out in an angular range of ±10° to ±50° and illuminates an area at 5 meters in an angular range of at least ±5° with intensity fluctuations of less than 50%. A condenser is disposed between the coupling-out surface of the light directing element and the object to be viewed. The condenser has an aperture with an aperture dimension and is disposed such that the aperture is irradiated completely with the light coupled out from the coupling-out surface. | 03-21-2013 |
| 20130070336 | MICROSCOPE APPARATUS HAVING A GOOSENECK OPERATING UNIT - A microscope apparatus includes a microscope, a gooseneck holder, and an operating unit configured to control apparatus functions of the microscope apparatus. The operating unit is carried by the gooseneck holder. | 03-21-2013 |
| 20130088777 | Retractable Beam Splitter for Microscope - Systems and methods are provided for illuminating a surface to be observed microscopically using a retractable beamsplitter. The retractable beamsplitter allows the use of coaxial illumination when the beamsplitter is positioned in the operator's line of sight. The retractable beamsplitter allows the use of non-coaxial illumination without reducing the amount of illumination that reaches the operator when the beamsplitter is retracted from the operator's line of sight. As a result a single system can be used effectively to provide various types of illumination. | 04-11-2013 |
| 20130094077 | STRUCTURAL ILLUMINATION AND EVANESCENT COUPLING FOR THE EXTENSION OF IMAGING INTERFEROMETRIC MICROSCOPY - In accordance with the aspects of the present disclosure, a method and apparatus is disclosed for imaging interferometric microscopy (IIM), which can use an immersion medium to enhance resolution up to a resolution of linear systems resolution limit of λ/4n, where λ is the wavelength in free space and n is the index of refraction of a transmission medium. | 04-18-2013 |
| 20130094078 | ILLUMINATING ARRANGEMENT FOR A MICROSCOPE - Illuminating arrangement for a microscope ( | 04-18-2013 |
| 20130100525 | OPTICAL IMAGING SYSTEM USING STRUCTURED ILLUMINATION - The present invention discloses an optical system to generate incoherent structured illumination and an optical imaging system using incoherent structured illumination. The optical system includes: at least one coherent light source, a spatial light modulator, a plurality of optical lenses, a rotating diffuser for destroying the coherence of the structured illumination pattern, an objective, and a stage accommodating samples. The optical imaging system using incoherent structured illumination includes: an optical microscope having an objective and a beam splitter, a charge-coupled device camera for recording a sequence of images of the samples, a stage for accommodating and moving samples; a coherent light source; a spatial light modulator; a quarter wave plate, a plurality of optical lenses and mirrors; and a diffuser rotating 360 degrees or vibrating rapidly around the axis of the optical path continuously. | 04-25-2013 |
| 20130107358 | METHOD AND SYSTEM FOR ILLUMINATING A SAMPLE | 05-02-2013 |
| 20130107359 | FAST PINHOLE CHANGER FOR CONFOCAL MICROSCOPY OR SPATIAL FILTER | 05-02-2013 |
| 20130120833 | MICROSCOPE SYSTEM - A microscope system includes a microscope body having a base portion forming a foundation, an arm portion extending substantially parallel to a bottom surface of the base portion, and a frame portion connecting ends of the base portion and the arm portion, having substantially a C shape in side view and holding an illumination optical system ejecting illumination light from a light source to a specimen. A light source unit is connected with the microscope body and radiates illumination light to the illumination optical system. A focusing unit supports a stage for placing the specimen and at least holding an objective lens focusing the specimen by collecting observation light from the specimen on the stage. The microscope body and the focusing unit do not contact each other in a state where an optical axis of the objective lens coincides with an optical axis of the illumination light. | 05-16-2013 |
| 20130128346 | MICROSCOPE DEVICE - A microscope device according to the present disclosure (the present microscope device) includes: a light source configured to oscillate coherent illuminating light, the illuminating light being applied on a specimen; a detecting unit configured to detect fluorescent light from the specimen as feedback light, the specimen being irradiated with the illuminating light; a phase distribution control unit disposed in an optical path of the illuminating light, the phase distribution control unit being configured to control phase distribution of the illuminating light; a controller configured to control the phase distribution control unit to vary the phase distribution; and an image generating unit configured to operate a difference of the feedback light between before and after the phase distribution varies, to generate an image of the specimen. | 05-23-2013 |
| 20130135715 | CHROMATIC CONFOCAL MICROSCOPE SYSTEM AND SIGNAL PROCESS METHOD OF THE SAME - A chromatic confocal microscope system and signal process method is provided to utilize a first optical fiber module for modulating a light into a detecting light passing through a chromatic dispersion objective and thereby forming a plurality of chromatic dispersion lights to project onto an object. A second optical fiber module conjugated with the first optical fiber module receives a reflected object light for forming a filtered light, which is split into two filtered lights detected by two color sensing units for generating two sets of RGB intensity signals, wherein one set of RGB intensity signals is adjusted relative to the other set of RGB intensity signals. Then two sets of RGB intensity signals are calculated for obtaining a maximum ratio factor. Finally, according to the maximum ratio factor and a depth relation curve, the surface profile of the object can be reconstructed. | 05-30-2013 |
| 20130135716 | OPTICAL MEMBER AND MICROSCOPE - An optical member and a microscope that allow acquiring brighter and sharper images when fluorescent observation is performed while stimulating a sample with light. Illumination light from a laser unit is split into stimulation light and excitation light by a dichroic mirror. In other words, half of the illumination light is transmitted through the dichroic mirror and becomes the stimulation light, and half of the illumination light is reflected by the dichroic mirror and becomes the excitation light. Half of the excitation light is reflected by a dichroic mirror and is irradiated onto a sample, and half of the stimulation light transmits through the dichroic mirror and is irradiated onto the sample. Fluorescence generated from the sample is totally reflected by the dichroic mirror and the dichroic mirror, and is received by a photodetector. | 05-30-2013 |
| 20130148196 | MICROSCOPE WITH TUNABLE ACOUSTIC GRADIENT INDEX OF REFRACTION LENS ENABLING MULTIPLE FOCAL PLAN IMAGING - An apparatus, system and method for microscopy. The apparatus, system and method includes a stage configured to receive an item; a tunable acoustic gradient index of refraction (TAG) lens having a first aspect positioned to image the received item, wherein the first aspect of the TAG lens is configured to have an optical power profile in accordance with an operational frequency of the TAG lens; one or more lenses configured to magnify an image of the received item at a viewing point; and at least one pulsed light source configured to illuminate the received item and to pulse at one or more points within the optical power profile of the TAG lens. | 06-13-2013 |
| 20130155499 | Pathology Slide Scanner - An instrument and method for scanning at least a portion of a large specimen preferably causes the specimen to move relative to a two-dimensional detector array at a constant speed. The detector array takes one image of the specimen for each line that the detector moves. A controller controls a shutter of the detector array to open to take images and to pass the images to a processor, which is preferably a computer. The instrument takes one partial image of each part of the specimen that is being scanned and then combines those images with other images to produce a contiguous image. | 06-20-2013 |
| 20130155500 | LASER SCAN CONFOCAL MICROSCOPE - Fluorescence is generated from an irradiated point on an inspection surface of a sample and the fluorescence is collected by an objective lens. Here, because of the magnification chromatic aberration of the objective lens | 06-20-2013 |
| 20130163077 | LIGHT MICROSCOPY METHOD AND A KIT FOR CARRYING OUT THE METHOD WITH A LIGHT MICROSCOPE - Described herein is a kit that includes at least one press for pressing matter that is being observed by a light microscope and a method of pressing the matter using the press while the matter is being observed. The press includes a pestle that is supported between, and for independent motion relative to the objective of the microscope and a sample residing on the stage of the microscope. | 06-27-2013 |
| 20130170023 | Device for Continuous Adjustment of Spectrometer Gap Widths - A microscope ( | 07-04-2013 |
| 20130176618 | MICROSCOPE AND MICROSCOPE LIGHT SOURCE UNIT - A microscope includes: a first epi-illumination light-source unit to perform fluorescence observation; a second transmitted-illumination light-source unit to perform transmission observation, the second transmitted-illumination light-source unit including a light source provided with a light emitting element that emits excitation light and a fluorescent substance that emits fluorescence upon irradiation with the excitation light; and an incidence limiting section configured to limit an incidence of light on the light source from an outside of the second light source unit during a light-off period of the light emitting element. The incidence limiting section is configured to remove an incidence limitation of the light from the outside while the light emitting element is being lit. | 07-11-2013 |
| 20130188250 | MICROSCOPE SYSTEM - A microscope system that performs structured illumination includes a light source configured to emit illumination light, an objective lens that irradiates a specimen with the illumination light, a phase-modulation spatial light modulator that has a two-dimensional pixel structure, that is arranged at the pupil conjugate position of the objective lens on an illumination light path between the light source and the objective lens, and that is configured to modulate a phase of the illumination light for each pixel so as to form a fringe illumination pattern on the specimen on the basis of an optical parameter of at least one of the light source and the objective lens. | 07-25-2013 |
| 20130201552 | SPECIAL-ILLUMINATION SURGICAL STEREOMICROSCOPE - The present invention relates to a special-illumination surgical stereomicroscope ( | 08-08-2013 |
| 20130250407 | DEVICE FOR INCREASING THE DEPTH DISCRIMINATION OF OPTICAL IMAGING SYSTEMS - The invention relates to a device for increasing the depth discrimination of optical imaging systems. Such a device comprises means for structuring illumination light in an illumination beam of the optical imaging system by means of a grating-like pattern in a conjugated object plane of the optical imaging system. The device further comprises means for varying the position of the grating-like patterns along an optical axis of the illuminating beam, and means for varying the position of an image of the grating-like pattern on a specimen in an object plane. | 09-26-2013 |
| 20130271829 | IMMERSION MICROSCOPE OBJECTIVE AND MICROSCOPE USING THE SAME - A microscope objective comprises in order from an object side, a first lens group, a second lens group, and a third lens group. The first lens group includes a first cemented lens, and at least one positive single lens, the second lens group includes a second cemented lens, and the third lens group includes a first lens component and a second lens component. A positive lens and a meniscus lens are cemented in the first cemented lens. A surface nearest to an image side of the first lens component is a concave surface, and a surface nearest to the object side of the second lens component is a concave surface. The first lens component and the second lens component are disposed such that the both concave surfaces are face-to-face, and the following conditional expression (1) is satisfied. | 10-17-2013 |
| 20130314777 | COLLIMATOR LENS, ILLUMINATION DEVICE, AND MICROSCOPE - A collimator lens is configured with a double convex single lens formed with a resinous material, for collimating a light flux emitted from a light source. At least one of surfaces of the collimator lens is formed as an aspherical surface. The collimator lens satisfies following conditions: | 11-28-2013 |
| 20130321908 | Sparse Deconvolution Spatial Light Microscopy in Two and Three Dimensions - Methods and a computer program product for applying deconvolution to spatial light interference microscopy for resolution enhancement with respect to the diffraction limit in two and three dimensions. By exploiting the sparsity properties of the phase images, which is prominent in many biological imaging applications, and modeling of the image formation via complex fields, the very fine structures can be recovered which were blurred by the optics. The resolution improvement leads to higher accuracy in monitoring dynamic activity over time. Experiments with primary brain cells, i.e. neurons and glial cells, reveal new subdiffraction structures and motions. This new information can be used for studying vesicle transport in neurons, which may shed light on dynamic cell functioning. Finally, the method may flexibly incorporate a wide range of image models for different applications and can be utilized for all imaging modalities acquiring complex field images. | 12-05-2013 |
| 20130329284 | Method and Configuration for the Optical Detection of an Illuminated Specimen - A method for the optical detection of an illuminated specimen, wherein the illuminating light impinges in a spatially structured manner in at least one plane on the specimen and several images of the specimen are acquired by a detector in different positions of the structure on the specimen. An optical sectional image and/or an image with enhanced resolution is then calculated. The method includes generating a diffraction pattern in the direction of the specimen in or near the pupil of the objective lens or in a plane conjugate to the pupil. A phase plate with regions of varying phase delays is dedicated to the diffraction pattern in or near the pupil of the objective lens or in a plane conjugate to said pupil, and different phase angles of the illuminating light are set. | 12-12-2013 |
| 20130335818 | Scanning Microscope, and Method for Light Microscopy Imaging of a Specimen - A scanning microscope is described, having an illumination unit for emitting an illumination light beam, an objective for generating an elongated illumination focus in a specimen to be imaged, and a scanning apparatus for moving the illumination focus over a target region of the specimen to be illuminated by modifying the direction of incidence in which the illumination light beam is incident into an entrance pupil of the objective. The scanning apparatus directs the illumination light beam onto a sub-region of the entrance pupil offset from the pupil center in order to incline the illumination focus relative to the optical axis of the objective, and modifies the direction of incidence of the illumination light beam within that sub-region in order to move the illumination focus over the target region to be illuminated. | 12-19-2013 |
| 20140029090 | RETRACTABLE BEAM SPLITTER FOR MICROSCOPE - Systems and methods are provided for illuminating a surface to be observed microscopically using a retractable beamsplitter. The retractable beamsplitter allows the use of coaxial illumination when the beamsplitter is positioned in the operator's line of sight. The retractable beamsplitter allows the use of non-coaxial illumination without reducing the amount of illumination that reaches the operator when the beamsplitter is retracted from the operator's line of sight. As a result a single system can be used effectively to provide various types of illumination. | 01-30-2014 |
| 20140029091 | MICROSCOPE AND METHOD FOR OPERATING A MICROSCOPE - The invention relates to a method for operating a microscope in which excitation light is focused on, or beamed to, different points of a specimen, in which an intensity of the excitation light is point-specifically varied and in which an intensity of the light reflected by said specimen in at least one spectral range is measured point-specifically and quantitatively. The method according to the invention is characterized in that the intensity and/or a spectral composition of the excitation light beamed to a specific point of said specimen is automatically adjusted by a regulating device on the basis of information previously gained from measured data of said specimen concerning an estimated or actual intensity of the light reflected in the spectral range by said point such that an integral of the intensity of the light reflected in the spectral range by this point during a pixel dwell time is within a predefined value interval. The invention also relates to a microscope. | 01-30-2014 |
| 20140049817 | FLUORESCENCE OBSERVATION DEVICE, DOMED BASE AND FLUORESCENCE MICROSCOPE PROVIDED WITH MULTIPLE LIGHT SOURCES HAVING DIFFERENT ILLUMINATION ANGLES - The present invention relates to a fluorescence observation device, a domed base and a fluorescence microscope. The fluorescence observation device includes a dome, a multi-angle light-emitting unit and a controller unit. The dome includes a transparent thermal insulation layer and a heat sink layer. The multi-angle light-emitting unit includes multiple directional narrow light field electro-luminescence devices thermally connected to the heat sink layer and adapted to emit light at different angles to pass through the transparent thermal insulation layer. The narrow light field electro-luminescence devices are selectively activated to emit light by the controller unit, so that the illumination angle of the multi-angle light-emitting unit can be adjusted. The fluorescence observation device can be combined with a base to constitute the domed base. Alternatively, narrow light field electro-luminescence devices are mounted on the base. | 02-20-2014 |
| 20140055851 | LASER SCANNING MICROSCOPE - A laser scanning microscope having a laser light source for fluorescence excitation, in particular a short pulse light source for multiphoton excitation; a scanning mirror array for scanning illumination of a specimen and a scanning optics for the generation of a diffraction-limited reference image plane as a first intermediate image plane; an optical system, preferably with a high numerical aperture, for the preferably demagnified imaging of the reference plane in a second intermediate image; an axially slideable mirror in the second intermediate image plane; a beam splitter array between the reference image plane and said optical system; and a tube lens and a first microscope objective for aberration-free imaging of the reference image plane in a specimen. Imaging of the image M of the second intermediate image plane in the specimen is effected with a magnification of M≠n/n′ and/or a magnification M of the second intermediate image plane in the specimen according to the equation M=y′/y=n/n′ξ, with |ξ|≠1 deviating by ξ≠1 from the quotient of the refractive indexes n, n′ of the immersion media in the object space on the specimen and in the image space on the mirror, and/or the focal plane of the excitation laser generated by the optical system deviates from the axial position of the mirror across basically the entire scanning area of the mirror in the intermediate image plane, such that the maximum energy density in the laser spot on the mirror surface area remains constantly below the destruction threshold of the reflecting layer and/or the image of the mirror surface area in the object space is located at least four depths of field from the focal plane of the laser in the object space and/or the second intermediate image exhibits strong aberrations. | 02-27-2014 |
| 20140055852 | LASER SCANNING MICROSCOPE - A laser scanning reflection or fluorescent microscope is provided with focusing-detecting unit having a laser beam focusing objective, an image detector that detects light reflected from the sample or back fluoresced light emitted by the sample, and a drive that simultaneously displaces the objective and the image detector. | 02-27-2014 |
| 20140085715 | Diffraction Phase Microscopy with White Light - A microscope and methods for obtaining a phase image of a substantially transparent specimen. Light collected from a specimen illuminated by a temporally incoherent source is diffracted into a first order and either the zeroth or first order is low-pass filtered in a Fourier transform plane before the orders are recombined at a focal plane detector. By low pass filtering the first order diffracted beam into a plurality of wavelengths, a spectrally- and spatially-resolved quantitative phase image of the specimen is obtained. | 03-27-2014 |
| 20140104680 | Total Internal Reflectance Fluorescence (TIRF) Microscopy Across Multiple Wavelengths Simultaneously - A multiple wavelength total internal reflection fluoresence (TIRF) microscopy system has an objective. A dispersion unit of the system comprises a high-dispersion optical element. The dispersion unit receives illumination light having at least a first wavelength λ | 04-17-2014 |
| 20140104681 | Spatial Filter to Combine Excitation Light and Emission Light in an Episcopic Multiplexed Confocal Scanning Microscope - In an episcopic multiplexed confocal scanning microscope system, a spatial filter is placed at a conjugate plane to a back aperture plane at a back aperture of an objective lens. The spatial filter is used to combine excitation light and emission light from a multiplexed confocal microscope module. | 04-17-2014 |
| 20140126046 | OPTOMECHANICAL MODULE FOR CONVERTING A MICROSCOPE TO PROVIDE SELECTIVE PLANE ILLUMINATION MICROSCOPY - A module for engagement with a conventional microscope to provide selective plane illumination microscopy is disclosed. The module is coupled to the translational base of the microscope and defines a mounting having a mount body in which an excitation objective having a first longitudinal axis is engaged to one portion of the mount body and a detection objective having a second longitudinal axis is engaged to another portion of the mount body such that the first longitudinal axis is in perpendicular geometric relation with the second longitudinal axis. | 05-08-2014 |
| 20140126047 | SPHERICAL ABERRATION CORRECTION FOR NON-DESCANNED APPLICATIONS - Spherical aberration is the primary cause of lose of signal while imaging deeper into a sample. Spherical aberration is corrected in the imaging path of a non-descanned detection system (such as a multi-photon microscope). This corrects the illumination spot for artifacts caused by imaging deep into a sample. One exemplary advantage to this instrument is that it allows deeper and brighter imaging. | 05-08-2014 |
| 20140133016 | ILLUMINATION OPTICAL SYSTEM AND MICROSCOPE - The illumination optical system illuminates an object plane on which a sample is placed in a microscope. The illumination optical system includes three light source areas arranged apart from one another in a pupil plane of the illumination optical system and being coherent with one another. Distances from centers of the three light source areas to a center of a pupil of the illumination optical system are different from one another. A condition of | 05-15-2014 |
| 20140133017 | Imaging Distal End of Multimode Fiber - Where a multimode fiber is used for light delivery in a microscope system and a transverse distribution of light exiting a distal end of the fiber is substantially uniform, the distal end is imaged onto a plane of a sample to be probed by the microscope system, or at a conjugate plane. Alternatively, the distal end is imaged onto a plane sufficiently close to the sample plane or the conjugate plane such that a radiant intensity of light at the sample plane or the conjugate plane is substantially uniform. In the case of a multi-focal confocal microscope system, the distal end of the multimode fiber is imaged onto a plane of a segmented focusing array. Alternatively the distal end is imaged onto a plane sufficiently close to the segmented focusing array plane such that a radiant intensity of the light at the segmented focusing array plane is substantially uniform. | 05-15-2014 |
| 20140160559 | llumination System - The invention is a system and method for controllable alignment of any of a plurality of electro-optical components mounted to a circuit board with an optical axis. In one embodiment, the invention provides an improved illumination system for microscopy. The system incorporates a circuit board 31 providing structure and directly mounting a plurality of light emitting sources. The mounted light sources are rotatably alignable with a plurality of optical axes. The system further includes selectable conditioning of the sources. | 06-12-2014 |
| 20140177044 | Method and Configuration for the Optical Detection of an Illuminated Specimen - A method for the optical detection of an illuminated specimen, wherein the illuminating light impinges in a spatially structured manner in at least one plane on the specimen and several images of the specimen are acquired by a detector in different positions of the structure on the specimen. An optical sectional image and/or an image with enhanced resolution is then calculated. The method includes generating a diffraction pattern in the direction of the specimen in or near the pupil of the objective lens or in a plane conjugate to the pupil. A phase plate with regions of varying phase delays is dedicated to the diffraction pattern in or near the pupil of the objective lens or in a plane conjugate to said pupil, and different phase angles of the illuminating light are set. | 06-26-2014 |
| 20140192406 | LASER SCANNING MICROSCOPE HAVING AN ILLUMINATION ARRAY - The invention relates to a laser scanning microscope (LSM), consisting of at least one light source, from which an illumination beam path in the direction of a sample originates, at least one detection beam path for passing sample light, preferably fluorescence light, onto a detector arrangement, it main colour separator for separating the illumination and detection beam paths, a microlens array for generating a light source grid composed of at least two light sources, a scanner for generating a relative movement between the illumination light and the sample in at least one direction, and a microscope objective, wherein the lens array is arranged in at common part of illumination and detection beam paths. | 07-10-2014 |
| 20140192407 | Method and Apparatus for Shaping Dynamic Light Beams to Produce 3D Perception in a Transmitted Light Microscope - An illuminator for a transmitted light microscope for producing stereo viewing as well as motion parallax 3D perception, using a generally pyramid-shaped mirror having a plurality of facets and light source adjacent a plurality of the facets and a computer-driven electronic circuit for controlling the on/off status of said light sources. | 07-10-2014 |
| 20140211305 | LASER SCANNING MICROSCOPE - A laser scanning microscope (LSM) consisting of at least one light source from which an illumination beam path extends in the direction of a sample, at least one detection beam path for transmitting sample light to a detector array, a first pinhole for confocal filtering in front of the detector array, a scanner for causing a relative motion between the illumination light and the sample in at least one direction, and a microscope lens. For illuminating a sample, at least two illumination beams, which the microscope lens focuses as illumination points in a sample plane, are generated in the illumination beam path. The laser scanning microscope is characterized in that in addition to the preferably adjustable, slit-shaped first pinhole, a second, preferably adjustable, slit-shaped pinhole is arranged downstream of the first pinhole so as to create optically conjugate beams arranged between the first and the second pinhole. | 07-31-2014 |
| 20140211306 | FLUORESCENCE OBSERVATION SYSTEM AND SET OF FILTERS - A set of filters for fluorescence observation comprises an illumination light filter and an observation light filter, wherein the following is holds: | 07-31-2014 |
| 20140211307 | LASER SCAN CONFOCAL MICROSCOPE - Fluorescence is generated from an irradiated point on an inspection surface of a sample and the fluorescence is collected by an objective lens. Here, because of the magnification chromatic aberration of the objective lens, the fluorescence going out from the objective lens travels along a path shifted from the irradiation light and changed substantially into a non-scan light by a galvano-scanner. The fluorescence passes through a dichroic mirror into a plane-parallel plate after light of unnecessary wavelength is removed by a filter. The plane-parallel plate is driven in synchronization with the galvano-scanner by a computer and corrects the shift and inclination of the optical axis generated by the magnification chromatic aberration of the objective lens. Then, the fluorescence forms an image of the irradiation point of the inspection surface of the sample on a pin hole of a pin hole plate by using a collective lens. | 07-31-2014 |
| 20140218793 | APPARATUS FOR MICROSCOPIC DETECTION OF HARDNESS - An adjustable stage mount includes a housing having a base defining a hole and an adjustable stage including a ball joint extension that rotatably engages the hole and is rotatably securable about the x-, y-, and z-axes. An adjustable indenter mount includes a housing defining a hole and an adjustable indenter including a ball joint extension that rotatably engages the hole and is rotatably securable about the x-, y-, and z-axes. A collision protection switch includes a first plate having three pairs of electrically conductive spaced apart pins wired to a voltage source in an open circuit, and a second plate having three electrically conductive balls. A spring pulls the first and second plates together causing the three balls to complete the circuit. A sufficient force against the second plate causes a ball to disengage and open the circuit. A two-objective microscope includes two parallel objectives, upper and lower light sources, three half-mirrors, and a camera. The camera and the half-mirrors are configured such that the camera views through either objective depending on which light source is on. | 08-07-2014 |
| 20140218794 | Confocal Fluorescence Microscope - A confocal fluorescence microscope of the present invention consists of: a light source unit broadly comprising one or more short wavelength laser beams; a lens unit which converts parallel light, emitted from a light source, into linear light having an appropriate size; a multi-color mirror which reflects the light source and enables fluorescence to transmit so as to separate the light source and the fluorescence; a scan mirror which radiates the light source over a wide area and scatters the fluorescence over a large-area camera; a microscope unit which radiates the incident light source to a target object, collects the fluorescence emitted from the target, and outputs the collected fluorescence; and a detecting unit which removes the background of the outputted fluorescent signal and observes the outputted fluorescent signal. | 08-07-2014 |
| 20140233095 | BROAD-SPECTRUM ILLUMINATOR FOR MICROSCOPY APPLICATIONS, USING THE EMISSIONS OF LUMINESCENT MATERIALS - A broad-spectrum, multiple wavelength illuminator comprises a luminescent body, and a plurality of semiconductor chips spaced apart from the luminescent body emitting light within one or more wavelength ranges towards the luminescent body, causing the luminescent body to emit light of one or more wavelength ranges. An optical element adjacent to the luminescent body collects light emitted by the luminescent body. An optical device collects light collected by the optical element. An aperture located between the optical element and the optical device passes the light emitted by the luminescent body along an optical axis, wherein light collected by the optical element and the optical device and passed by the aperture forms a beam of light illuminating a target. Alternatively, instead of being spaced apart from the chips, the luminescent body may be a layer adjacent to the chips. | 08-21-2014 |
| 20140233096 | Low Numerical Aperture Exclusion Imaging - In accordance with one embodiment of the present invention an apparatus for a low numerical aperture exclusion imaging apparatus is provided. The apparatus may include an electromagnetic illumination source for illuminating a portion of a specimen; and for collecting an image created by the electromagnetic radiation an objective lens optically coupled to the electromagnetic illuminated portion of the specimen. The apparatus also includes an optical blocking plate disposed between the objective lens and a focusing lens. The optical blocking plate is positioned to substantially block undesired electromagnetic radiation from image sources distally aligned in the same optical axis as the specimen. This invention is enhances narrow depth of field characteristics in imaging. It also enhances discreet imaging in a narrow focus field by eliminating some or most of the light which contributes to wide depth of field focus. This is useful for optical sectioning ranging from microscopy to photography. Optical sectioning provides the information necessary for 3D image reconstructions and other X Axis spatial measurements. | 08-21-2014 |
| 20140254005 | Microscope and Method for SPIM Microscopy - A method for SPIM microscopy, wherein the sample is moved continuously, and a plurality of images are taken at time intervals by means of a detection arrangement during the movement. The image capture duration or exposure time is dimensioned such that the movement path of the sample lies within a predetermined resolution range of the detection objective. The speed of the sample movement is determined and set by the image capture duration or exposure time and/or the distortion of the point spread function generated by the sample movement of the sample. The image blur is corrected computationally by the respective image capture duration and the movement speed. A sharp image is generated in this way. The actual optical section thickness of the light sheet is determined from the light sheet thickness, and the movement speed is determined therefrom and from user settings. | 09-11-2014 |
| 20140293410 | LASER SCANNING MICROSCOPE - A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity. | 10-02-2014 |
| 20140293411 | MICROSCOPE - A microscope includes: a light source configured to generate light for irradiating a specimen; a microscope main body which supports the light source and on which an accommodation unit that accommodates the specimen is placed; a plurality of optical units, each of which is detachably installed in the microscope main body, configured to arranged on an optical path of the light, and configured to change optical characteristics of the light incident thereon; an operation input unit that includes a plurality of input units configured to respectively receive inputs of drive signals for driving optical units to be controlled among the plurality of optical units; and a control unit configured to allocate optical units to be respectively driven with the drive signals by the plurality of input units according to the plurality of optical units installed in the microscope main body. | 10-02-2014 |
| 20140293412 | MODULAR MICROSCOPE CONSTRUCTION - A system, apparatus and method for using modular microscopes is disclosed. Connecting the housings of the individual microscope modules provide the structural framework of the modular microscope. Furthermore, the modular microscope can include specialized software, the distribution and use of which can be controlled using security keys or identifiers stored on one or more of the microscope modules. The security keys and identifiers can be based on calibration data associated with the physical, electrical, or optical properties of one of more of the modules. The illumination modules disclosed provide for selectable wavelengths and controllable levels of output illumination for both bright field and dark field illumination. | 10-02-2014 |
| 20140300958 | ARRANGEMENT FOR USE IN THE ILLUMINATION OF A SPECIMEN IN SPIM MICROSCOPY - An arrangement for use in illuminating a sample in SPIM microscopy includes an illumination objective configured to receive and focus a light strip or a quasi-light strip. The quasi-light strip is made up of a light bundle continuously moved back and forth in a light-strip plane. A deflection apparatus is configured to deflect the light strip or the quasi-light strip, after the light strip or the quasi-light strip has passed through the illumination objective, in such a way that the light strip or the quasi-light strip propagates at an angle different from zero degrees with respect to an optical axis of the illumination objective. The illumination objective and the deflection apparatus are arranged movably relative to one another. | 10-09-2014 |
| 20140313575 | MICROSCOPE ADAPTER UNIT - A microscope adapter unit disposed on an optical path of illumination light between a light source unit including a light source and a sample surface includes a first lens group having at least one lens and a second lens group having at least one lens. The first lens group converts the illumination light into roughly parallel luminous fluxes, and makes the illumination light enter the second lens group. | 10-23-2014 |
| 20140313576 | Microscope Device - A microscope has an objective, a light source illuminating a sample over an illumination beam path, an arrangement producing a flat illumination pattern which is structured in both spatial directions on the sample, a surface detector detecting light coming over one picture beam path, an arrangement shifting the illumination pattern on the sample in one displacement direction, and a control unit taking one picture at a time of the light which was detected by the detector as phase picture in different positions of the pattern along the displacement direction and to computationally reconstruct from these phase pictures an overall picture of the illuminated sample region. The displacement direction is oblique to the main axes of symmetry of the illumination pattern and depending on the illumination pattern is chosen such that the number of phase pictures which is necessary for the picture reconstruction corresponds to the theoretically minimally required value. | 10-23-2014 |
| 20140327960 | MICROSCOPE AND STIMULATING APPARATUS - A scanning microscope includes: a first scanning optical system that irradiates a specimen with light from a first light source via an objective lens to receive light from the specimen; and a second scanning optical system that irradiates the specimen with the light from the first light source or light from a second light source different from the first light source via the objective lens so as to cause the specimen to express a specific phenomenon. The second scanning optical system has a beam shaping optical system that shapes the light from the first light source or the light from the second light source such that a light convergence region on which the light from the first light source or the light from the second light source is collected via the objective lens satisfies a predetermined condition. | 11-06-2014 |
| 20140347723 | Three Dimensional Stimulated Emission Depletion Microscopy - A STED microscope for producing a 3D image. The microscope has an excitation laser source for providing an excitation laser beam and a depletion laser source for providing stimulated emission depletion laser beam. The excitation beam is capable of exciting fluorescent markers in a sample placed in the sample region and the depletion beam is capable of inducing stimulated emission in the fluorescent marker in the sample in a region around the excitation beam to restrict the size of the region within which the fluorescent markers are excited and wherein the depletion laser source goes through an optical element which creates a cone refractive pattern which induces stimulated emission in the fluorescent marker in the sample. The shape of the light hollow of the cone refractive pattern varies at different distances from the source. This depth profile can be used to distinguish between positions at varying depths within a sample to create a 3D image. The combination of varying intensity across the sample and at different depths means that the present invention is able to produce images with lateral and axial resolutions ranging from the micro to the nano regime. | 11-27-2014 |
| 20140362435 | NONLINEAR OPTICAL MICROSCOPE APPARATUS - A nonlinear optical microscope apparatus includes: an objective for irradiating a laser beam on a sample; a beam diameter change unit for changing a beam diameter of the laser beam incident to the objective; and a control unit for deciding, for each of optical characteristics of the sample, a pupil filling ratio, which is a ratio of the beam diameter of the laser beam incident to the objective to a pupil diameter of the objective, based on the optical characteristics of the sample, and for controlling the beam diameter change unit so that the pupil filling ratio becomes the decided value. | 12-11-2014 |
| 20140368903 | Apparatus for Aiding Manual, Mechanical Alignment of Optical Equipment - A microscope subject illumination system including a position targeting accessory that identifies a point on a subject by a distinctive illumination pattern cast on the subject. The system includes an automatic control to switch between illumination sources so that the distinctive pattern is easier to discern during subject positioning. The control may automatically extinguish the targeting illumination after a configurable period of time. | 12-18-2014 |
| 20140368904 | Software Defined Microscope - A microscope having a first illumination spatial light modulator (SLM) that receives light of a first wavelength from an illumination source and processes that light in a manner that transfers light into an objective lens through a dichroic reflector that passes light of the first wavelength is disclosed. The microscope includes an imaging system that receives light from the objective lens and forms an image on a camera, and a controller having a graphical user input that displays the image to a user and controls the first illumination SLM to alter the processing of the light in response to commands from the user. The illumination SLM is controlled to provide functions that would normally be carried out by one or more lenses or prisms in the illumination optical train of a conventional microscope and/or correct for alignment errors in the illumination source. | 12-18-2014 |
| 20140376087 | IMAGE PROCESSING APPARATUS, MICROSCOPE SYSTEM, AND IMAGE PROCESSING METHOD - An image processing apparatus includes an image acquisition unit that acquires image information representing a fluorescence observation image of a specimen stained with hematoxylin-eosin, a spectrum generation unit that generates a plurality of spectra each representing a wavelength distribution of fluorescence intensity in a plurality of pixels in the fluorescence observation image, a pixel extraction unit that extracts at least two pixel groups with a feature of a particular spectrum from the plurality of pixels, and an image generation unit that generates an image based on the extracted pixel groups. | 12-25-2014 |
| 20150009558 | 4PI STED FLUORESCENCE LIGHT MICROSCOPE WITH HIGH THREE-DIMENSIONAL SPATIAL RESOLUTION - An apparatus forming an area of minimum light intensity enclosed by high light intensity includes two objectives focusing light of opposite directions into a common focal area. Light intensities of a first pair of light beams extinguish each other in a first partial area of the focal area. Beam paths of the first pair of beams pass through the objectives in a first pair of partial areas of the pupils of the objectives. Light intensities of a second pair of light beams extinguish each other in a second partial area of the focal area. Beam paths of the second pair of light beams pass through the objectives in a second pair of partial areas which are offset with regard to the first pair of partial areas of the pupils; and the light of the second pair does not interfere with the light of the first pair of light beams. | 01-08-2015 |
| 20150022881 | LIGHT SHEET-BASED IMAGING DEVICE WITH EXTENDED DEPTH OF FIELD - Light sheet microscope for recording 3D images of a specimen, comprising a light sheet generator, an objective lens, an image detector and a wavefront encoder element or system positioned between the objective lens and the image detector for extending the depth of field of the objective lens. By doing so, the restriction on the distance between the collecting objective and the light sheet is relaxed, allowing the use of a light sheet scanning unit. The resulting light sheet microscope allows for a robust, aberration insensitive, fast 3D imaging without the need to move or perturb the specimen. | 01-22-2015 |
| 20150022882 | MICROSCOPE SYSTEM, OBJECTIVE LENS UNIT, AND MICROSCOPE MAIN BODY - A microscope system includes: a main body portion having an optical system for forming an image from at least observation light from a specimen; an objective lens unit having an objective lens for taking in the observation light and having a base portion with an approximately belt shape for holding the objective lens on one end; and a unit attachment portion that is held by the main body portion and is configured to detachably attach the base portion at a position where at least an optical axis of the objective lens coincides with an optical path of the observation light. | 01-22-2015 |
| 20150062698 | Diffusing Collection Lens for Direct Coupled High Power Microscopy Illumination Systems - A high power microscopy illumination system is disclosed, including a solid state illumination source. A diffusing collection lens having a diffusing surface is configured to collect and diffuse light emission from the solid state illumination source. An emitting surface is disposed substantially opposite the diffusing surface. An optical coupling element couples the light emission from the diffusing collection lens emitting surface along an optical axis to an optical output. The diffusing collection lens provides improved uniformity of illumination with direct coupling, without significant power loss. | 03-05-2015 |
| 20150070756 | LASER SOURCE WITH A LARGE SPECTRAL RANGE - A laser source ( | 03-12-2015 |
| 20150070757 | Microscope - A microscope including an imaging objective for imaging a sample on a detector and means for illuminating the sample with a light sheet in the focus plane of the imaging objective. The illumination means includes an illumination source which emits coherent light, and Bessel optics which generate at least two plane waves from the light beam and give propagation directions for the plane waves. The propagation direction of each of the plane waves encloses an acute angle with the focus plane in each instance, the magnitude of the acute angle being identical for each of the plane waves, so that the plane waves undergo constructive interference in the focus plane so that a light sheet is generated. Similarly, the illumination means can also include an optical element by which a rotationally symmetric Bessel beam is generated from the light beam for dynamic generation of a light sheet. | 03-12-2015 |
| 20150077844 | LASER SCANNING MICROSCOPE AND METHOD FOR CORRECTING IMAGING ERRORS PARTICULARLY IN HIGH-RESOLUTION SCANNING MICROSCOPY - A laser scanning microscope (SR-LSM) and a method for correcting imaging errors in a laser scanning microscope. The SR-LSM includes an illumination device for providing an illumination spot; a scanner for moving the illumination spot to consecutive scanning positions over a sample to be examined; an adaptive optics unit for controlling a wavefront of the illumination spot with a control device and a detector for determining a spatially resolved imaging spot emitted by the sample. An evaluation unit is provided for determining a point-spread function (PSF) of the imaging spot at each scanning position, whereby a wavefront correction signal determined from the point-spread function (PSF) of a scanning position is supplied to the control device of the adaptive optics unit or is used in digital post-processing of the microscope image (e.g. by means of deconvolution). | 03-19-2015 |
| 20150085359 | MICROSCOPE SUPER-RESOLUTION ILLUMINATION SOURCE - An alternative illumination source for traditional optical microscopes is provided. This super-resolution illumination source permits to use a traditional optical microscope for the direct observation of objects smaller than 100 nanometers such as subcellular structures in microorganisms, carbon nanotubes and other nanosize objects, and even nanostructures fabricated on top of a silicon wafer. This invention relies on the integration of two functional elements: a microscope, and the super-resolution illumination source. The super-resolution illumination source is formed by an array of light emitting diodes (LEDs) uniformly distributed in a hemisphere. The object under observation is illuminated by the light emitted in all directions by the array of LEDs. Real, wide-field images of the sample with nano-resolution are direct and analogically formed by the microscope's lenses, without the need of sample tagging, intensive computation or scanning. Depending on the wavelength emission of the LEDs, nano-resolution can be obtained with ultraviolet, infrared, and visible illumination. | 03-26-2015 |
| 20150085360 | CONFOCAL MICROSCOPE - A confocal microscope includes an illumination optical system configured to uniformly illuminate at least part of a first confocal stop with a light beam from a light source, a first collection optical system configured to collect a light beam passing through the first confocal stop onto a specimen, a second collection optical system configured to collect a light beam from the specimen onto a second confocal stop, a detection unit configured to detect a light beam passing through the second confocal stop, and a light intensity control member provided to at least one of the first collection optical system and the second collection optical system and having a transmittance of a first region including an optical axis that is lower than a transmittance of a second region around the first region. | 03-26-2015 |
| 20150098126 | Multiview Light-Sheet Microscopy - A method of imaging a live biological specimen includes generating one or more first light sheets; directing the generated one or more first light sheets along respective paths that are parallel with a first illumination axis such that the one or more first light sheets optically interact with at least a portion of the biological specimen in a first image plane; recording, at each of a plurality of first views, images of fluorescence emitted along a first detection axis; generating one or more second light sheets; directing the generated one or more second light sheets along respective paths that are parallel with a second illumination axis such that the one or more second light sheets optically interact with at least a portion of the biological specimen in a second image plane, and recording, at each of a plurality of second views, images of fluorescence emitted along a second detection axis. | 04-09-2015 |
| 20150116822 | LASER SCANNING MICROSCOPE APPARATUS AND LASER SCANNING METHOD - Both light stimulation of a specimen at a high intensity and detailed fluorescence observation at a low intensity are realized by using a single laser light source. Provided is a laser scanning microscope apparatus ( | 04-30-2015 |
| 20150293336 | Spatial Filter Enhanced Spinning Disk Confocal Microscope - A spatial filter includes a first focal plane to receive sample fluorescence and auto-fluorescence from a microscope, a first lens to receive the sample fluorescence and auto-fluorescence and focus rays of the sample fluorescence, a mask aperture positioned in a plane where sample fluorescence rays maximally converge, the mask aperture positioned where such rays converge to pass the rays, the aperture having a size that is a function of characteristics of the microscope, and a second lens positioned to receive the passed rays from the spatial filter and form images at a second focal plane to couple to a camera. | 10-15-2015 |
| 20150293339 | ILLUMINATION DEVICE - An illumination device for an optical device, a microscope or a macroscope includes a first illumination source configured to emit light which is directed via an illumination beam path onto an object to be illuminated that is arranged in an object plane. At least one second illumination source is positionable in the illumination beam path, and is transparent or semitransparent as well as self-luminous. The at least one second illumination source is configured to allow light emitted from the first illumination source to pass through at least in part. The object plane having the object to be illuminated is configured to be illuminated both by the first and by the at least one second illumination source. | 10-15-2015 |
| 20150316758 | LIGHT IRRADIATION DEVICE - A light irradiation device is an apparatus for irradiating an irradiation object, and includes a light source outputting readout light L | 11-05-2015 |
| 20150331227 | ADJUSTING DEVICE FOR AN ILLUMINATION COMPONENT OF A MICROSCOPE, A MICROSCOPE ILLUMINATION DEVICE AND A MICROSCOPE - An adjusting device for an illumination component of a microscope, an illumination device and a microscope containing the adjusting device are disclosed. The adjusting device for an illumination component of a microscope includes a first barrel component having an axis; a second barrel component received in the first barrel component, the second barrel component having an axis coincident with or parallel to the axis of the first barrel component, wherein the second barrel component houses and supports the illumination component; and a plurality of set screws each having a first end coupled to a side wall of the first barrel component at a corresponding coupling point and a second end pressed against a side wall of the second barrel component. With embodiments of the present invention, the structure is simpler and the cost is lower. | 11-19-2015 |
| 20150338629 | MICROSCOPE WITH ILLUMINATION DEVICE - A microscope includes an illumination device having a light source and two current control devices. Each of the current control devices has a target value input and a measuring resistor arrangement. The current control devices supply a through-current for the light source and control a level thereof by way of a potential difference across the measuring resistor arrangement on the basis of a signal provided at the target value input. An adjustment device is configured to set two different illumination types of the microscope. The adjustment device and the illumination device are operatively connected to each other such that setting a first illumination type switches the illumination device into a first current configuration, in which at least one current control device supplies no through-current, and setting a second illumination type switches the illumination device into a second current configuration, in which at least one control devices supplies a through-current. | 11-26-2015 |
| 20150338630 | Device for marking a sample, and observation system comprising such a marking device - This marking device for marking a sample is intended to be placed facing the sample for observing the sample by means of an observation system. The marking device comprises a plurality of meshes forming a meshing, the marking device comprising at least one identification marking of each mesh. | 11-26-2015 |
| 20150346474 | ILLUMINATION APPARATUS, MICROSCOPE APPARATUS EQUIPPED WITH SAME, AND MICROSCOPY OBSERVATION METHOD - An illumination apparatus used for fluorescence observation of a sample containing a fluorescent material by a microscope apparatus, comprising an excitation light emission unit that emits excitation light for exciting the fluorescent material contained in the sample. The excitation light emission unit illuminates at least a bleaching reduction illumination region around an observed region in which the sample is present. | 12-03-2015 |
| 20150355444 | COMPACT LENS SYSTEM FOR USE IN PHOTOACOUSTIC MICROSCOPY - A lens system for use with photoacoustic microscopy apparatus has a collimated single mode optical fiber to which a toroidal ultrasound transducer is operatively attached. The transducer is located inside a tank. A lens housing is located inside the tank adjacent the transducer and has flexible optically transmissive entrance and exit ports made of polydimethysiloxane. The lens housing is filled with cinnamaldehyde. The cinnamaldehyde can be introduced into the lens housing and withdrawn from it so as to flex its entrance and exit ports, and the tank is filled with a mixture of glycerol and water. | 12-10-2015 |
| 20150378141 | LIGHT MICROSCOPE AND MICROSCOPY METHOD - A light microscope having a sample plane for positioning a sample, and a light source for emitting illumination light, includes optical imaging means for guiding the illumination light into the sample plane. A detector device having a plurality of detector elements for detecting sample light coming from the sample. Adjacent detector elements are at a distance from one another which is smaller than an Airy-Disk produced by a point of the sample plane on the detector device. A scanning device has at least a first and a second optical arrangement simultaneously movable in a common direction for producing an illumination scanning movement and a detection scanning movement, which are opposite to one another. Sample regions spaced apart from one another can be examined simultaneously, such that both a beam path of the sample light from the sample plane to the detector device and a beam path of the illumination light from the light source to the sample plane run via the first optical arrangement and only one of these two beam paths runs via the second optical arrangement. Sample light can be imaged in a non-inverting manner and with an imaging scale of less than one. The invention is additionally directed to a corresponding microscopy method. | 12-31-2015 |
| 20150378142 | ILLUMINATION DEVICE FOR AN OPTICAL VIEWING APPARATUS - An illumination device for an optical viewing apparatus has a light source including a first individual light source and a second individual light source arranged in a plane. The first individual light source has a first midpoint and the second individual light source has a second midpoint. An axial direction is defined by a vector from the second midpoint to the first midpoint. An illumination optical unit defines an optical axis which is arranged perpendicularly to the plane and intersects the plane at an intersection point. The light source is imaged toward infinity by the illumination optical unit. The first individual light source has an extent along the axial direction. The midpoint is offset by an amount (Δ) in a positive direction along the axial direction relative to the intersection. The relationship 0.1*L | 12-31-2015 |
| 20160048009 | MICROSCOPE APPARATUS AND APPLICATIONS THEREOF - In an aspect, a microscope removably attachable to a portable electronic device includes a lens including an optical axis and configured to magnify at least a portion of an object. The microscope further includes a member configured to support the lens and removably attach to the portable electronic device. In another aspect, a microscope for viewing an object situated on a slide includes a lens including an optical axis and configured to magnify at least a portion of the object situated on the slide. The microscope further includes a member supporting the lens. In such aspect, the member includes a holder platform configured to position and hold the slide at a distance from the lens. | 02-18-2016 |
| 20160048012 | Method and Optical Arrangement for Manipulating and Imaging a Microscopic Sample - The invention relates to a method in which a sample is manipulated with manipulation light, and in which the sample is imaged by means of the SPIM technique under illumination with illumination light, in particular excitation light for fluorescence excitation, in the form of an illumination light sheet. The method is notable for the fact that both the manipulation light and the illumination light are focused by the same objective that is arranged in an objective working position, or by different objectives that are brought successively into an objective working position; and that the manipulation light and/or the illumination light, after passing through the objective, is diverted by means of a diverting device in such a way that it propagates at an angle different from zero degrees with respect to the optical axis of the objective. | 02-18-2016 |
| 20160054552 | CONFOCAL LASER SCANNING MICROSCOPE - A confocal laser scanning microscope includes a laser light source configured to emit laser light as illumination light, an objective, a scanner placed in an optical path between the laser light source and the objective for scanning the sample with the illumination light, and a stop placed on a pupil plane of the objective or in its vicinity, or on a plane that is optically conjugate with the pupil plane of the objective or in its vicinity, configured to block, of light from the sample, light that is a regular reflection of the illumination light cast on the sample. The stop is placed on a plane that is conjugate with the pupil plane of the objective or in its vicinity in an optical path between the laser light source and the scanner. | 02-25-2016 |
| 20160070091 | MICROSCOPE MODULE FOR IMAGING A SAMPLE - A microscope module ( | 03-10-2016 |
| 20160103310 | TWO-PHOTON EXCITATED FLUORESCENCE MICROSCOPE - A two-photon excited fluorescence microscope including a laser source configured to emit a light beam and an optical arrangement configured to receive the light beam from the laser source. The optical arrangement is configured to shape the light beam so that, at an output of the microscope, the light beam is substantially collimated in a first transverse direction perpendicular to the propagation direction of the light beam at the microscope output and is focused in a second transverse direction perpendicular to the first transverse direction and to the propagation direction, thereby forming a line parallel to the first transverse direction. | 04-14-2016 |
| 20160109694 | LASER MICROSCOPE - With the object of expanding the dynamic range of an intensity signal and suppressing degradation of a light detecting portion, a laser scanning microscope of the present invention includes a light irradiating portion that irradiates a sample with laser light; a PMT that detects fluorescence from the sample and that outputs an intensity signal; a data processing portion that converts the intensity signal to brightness information at each pixel; a full-scale setting portion that can optionally set a normal full scale that is smaller than the intensity signal of the fluorescence from the sample when a light stimulus is given thereto and an expanded full scale that is larger than the normal full scale, as a maximum range of the intensity signal that can be converted to brightness information; and a control portion that determines whether to switch between a normal observation mode in which the brightness information of the sample when the light stimulus is not given thereto is obtained and a stimulation observation mode in which the brightness information of the sample when the light stimulus is given thereto is obtained; wherein the full-scale switching portion sets the normal full scale in the normal observation mode and sets the expanded full scale in the stimulation observation mode, on the basis of the result of the determination in the control portion. | 04-21-2016 |
| 20160124201 | LIGHT SHEET ILLUMINATION MICROSCOPE AND LIGHT SHEET ILLUMINATION METHOD - A light sheet illumination microscope includes a detection optical system and an illumination optical system. The illumination optical system includes a first optical element for forming a sheet-shaped illumination beam that travels in a first direction and that has a width in a second direction that is perpendicular to both the optical axis of the detection optical system and the first direction in a specimen, and a scanner that relatively scans the specimen with the sheet-shaped illumination beam in the second direction. | 05-05-2016 |
| 20160139394 | MICROSCOPE, FOCUSING UNIT, FLUID HOLDING UNIT, AND OPTICAL UNIT - A microscope includes: a sample placement part having a placement surface on which to place a sample and a bottom face opposite to the placement surface; an observation lens; and an optical unit for generating sheet light and use of the microscope. The microscope of an embodiment is arranged such that the sheet light enters the sample placement part from the bottom face and passing through the sample placement part to irradiate the sample, and fluorescence from the sample passes through the sample placement part toward the bottom face to be received by the observation lens. The microscope can, with this arrangement, utilize the advantages of a SPIM, and allows observation of a sample which observation is free from a restriction of the size of a sample. | 05-19-2016 |
| 20160139395 | SCANNING MICROSCOPE - A scanning microscope includes a scanner, an objective irradiates a sample with illumination light deflected by the scanner, and a beam splitter that is arranged between the objective and an exit pupil position, and that reflects one of the illumination light and observation light from the sample and transmits the other. The objective has the exit pupil position outside the objective. | 05-19-2016 |
| 20160153892 | METHOD AND OPTICAL DEVICE FOR MICROSCOPICALLY EXAMINING A MULTIPLICITY OF SPECIMENS | 06-02-2016 |
| 20160154236 | Assembly for Light Sheet Microscopy | 06-02-2016 |
| 20160170193 | MICROSCOPE SYSTEM AND SETTING VALUE CALCULATION METHOD | 06-16-2016 |
| 20160170195 | Arrangement for Light Sheet Microscopy | 06-16-2016 |
| 20160187633 | DUAL OBLIQUE VIEW SINGLE PLANE ILLUMINATION MICROSCOPE - A microscope includes an optical assembly including a first objective; and, another optical assembly including a second objective; wherein the first objective and the second objective are configurable to be oriented in an oblique angle relative to each other to provide for illumination and observation of a specimen. A method of fabrication and a controller are disclosed. | 06-30-2016 |
| 20160187635 | PHASE CONTRAST MICROSCOPE - A phase contrast microscope includes a light source, an illumination optical system configured to irradiate an observation object with the light from the light source, and a magnifying optical system configured to magnify and project the observation object, the illumination optical system includes a ring slit, the magnifying optical system includes an objective provided with a phase film, the ring slit is disposed at a pupil position of the illumination optical system, the phase film is disposed at a position conjugate to the pupil position of the illumination optical system, and the following conditional expression (1) is satisfied: | 06-30-2016 |
| 20160193604 | SYSTEMS FOR OPERATING ELECTROKINETIC DEVICES | 07-07-2016 |
| 20160252715 | DRIVE CONTROL METHOD FOR OBJECTIVE LENS AND FLUORESCENCE MICROSCOPE SYSTEM | 09-01-2016 |
| 20160377850 | MULTIFOCAL SCANNING FLUORESCENCE MICROSCOPE - Scanning fluorescence microscopes with an observation beam path from a measurement volume to an image plane. A beam combiner is provided for coupling an illumination system and a diaphragm arranged in the image plane for slow composition of the image because of the sequential scanning and subject the sample to loading as a result of inefficient use of the excitation light. The microscope simultaneously detects fluorescence from different focal planes in each case quasi-confocally. The observation beam path between the beam combiner and the image plane has a first diffractive optics for splitting light beams into beam bundles along different orders of diffraction, imparting to the light beams a spherical phase that is different from the other orders of diffraction. A second diffractive optics is provided for the compensation of chromatic aberrations of the split beam bundles, and a collecting optics is provided for focusing split beam bundles into the image plane. | 12-29-2016 |
| 20160377852 | IMAGING DEVICE FOR MICROSCOPE - An imaging device for a microscope ( | 12-29-2016 |
| 20160377875 | Apparatus and Method for Light Modulation - In order to modulate different spectral components of a light beam independently of one another, the light beam is split into a plurality of spectral components which can be modulated at different locations of a spatial light modulator. | 12-29-2016 |
| 20180024341 | AUGMENTED STEREOSCOPIC MICROSCOPY | 01-25-2018 |
| 20190143447 | PULSE LIGHT GENERATION DEVICE, LIGHT IRRADIATION DEVICE, OPTICAL PROCESSING DEVICE, OPTICAL RESPONSE MEASUREMENT DEVICE, MICROSCOPE DEVICE, AND PULSE LIGHT GENERATION METHOD | 05-16-2019 |
| 20190146202 | APPARATUS AND METHOD FOR LIGHT-SHEET-LIKE ILLUMINATION OF A SAMPLE | 05-16-2019 |
| 20220137384 | MICROSCOPE AND METHOD FOR LIGHT-FIELD MICROSCOPY WITH LIGHT-SHEET EXCITATION AND FOR CONFOCAL MICROSCOPY - A microscope, which includes a color splitter that is reflective to excitation radiation, can switch between a first and a second operating mode. A first apparatus can introduce a first cylindrical optical element into the excitation beam path between a light source and the color splitter, when the microscope is in the first operating mode, and a second apparatus can introduce a second cylindrical optical element into the excitation beam path between the color splitter and a scanning apparatus. | 05-05-2022 |
