| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 204548000 | Isoelectric focusing (i.e., using pH variation) | 20 |
| 20080202934 | Method and Apparatus for the Simultaneous Separation of Biological Molecules by Two Dimensional Electrophoresis - The patent describes a method for the simultaneous and repeatable separation of biological molecules by bidimensional electrophoresis and the apparatus usable to carry out thereof. And in particular, the present invention refers to a method and to an apparatus as defined above, capable to transfer and separate, on a matrix, of protein and/or polypeptide and/or peptide components that were previously ordered on another matrix according to specific chemical and/or physical characteristics. | 08-28-2008 |
| 20090026081 | METHOD, COMPOSITION AND KIT FOR ISOELECTRIC FOCUSING - The present invention relates to a method, composition and a kit for isoelectric focusing. More closely, the present invention involves the use of to modified carrier ampholytes which are easy to separate from the proteins/peptides following finished focusing. The modification is that the carrier ampholytes are derivatised with handles interacting with a solid phase/matrix which makes them easy to remove and separate from the peptides after finished focusing. In this way, there is very little or no background from carrier ampholytes in the following MS-spectra. | 01-29-2009 |
| 20090218224 | METHOD FOR ELECTROPHORESIS INVOLVING PARALLEL AND SIMULTANEOUS SEPARATION - An electrophoresis method is provided involving a parallel and simultaneous multiple process, using multiple separation sub-spaces within a separation chamber. | 09-03-2009 |
| 20100012497 | FLOW CONTROL SYSTEMS - A flow controller which uses a combination of hydrostatic pressure and electroosmotic flow to control the flow of a fluid. A driving fluid ( | 01-21-2010 |
| 20100108517 | Concentration and Cleanup of Nucleic Acid Samples - Methods and devices are described for concentration and cleanup of samples containing bio-molecule analytes (e.g., polynucleotides, such as DNA, RNA, PNA). Various embodiments provide for pH-mediated sample concentration and cleanup of nucleic acid samples with channel devices (e.g., cross-T format, microchannel devices). | 05-06-2010 |
| 20100270159 | Apparatus for Purifying Molecules - The invention provides devices for trapping and collecting separated biomolecules following a separation step. The invention also provides methods of using the devices to trap and collect separated biomolecules. | 10-28-2010 |
| 20100307920 | pH GRADIENTS CONTROLLED BY ELECTROLYSIS, AND THEIR USE IN ISOELECTRIC FOCUSING - A specified proton concentration in a volume ( | 12-09-2010 |
| 20110017599 | TARGET RECOGNITION MOLECULE AND A METHOD FOR IMMOBILIZING THE SAME - A novel target recognition molecule is provided which has a electrostatic property whereby such target recognition molecules can be densely brought together in a self-assembly manner in a predetermined region of an analytical chip and, in addition, can be reversibly or irreversibly stably immobilized there. This target recognition molecule has a target recognition peptide segment as a specific binding site for a target substance, an electrostatically-charged segment which is provided with three or more electrostatically-charged functional groups capable of being electrically charged with charges of the same polarity in the same solution and which has no functional groups that become electrically charged to different polarities in the same solution, and a connecting segment which chemically links with the target recognition peptide segment and with the electrostatically-charged segment for establishing a connection between both the segments. | 01-27-2011 |
| 20110062025 | ISOELECTRIC FOCUSING SYSTEMS AND METHODS - The invention relates to devices and methods for the separation of species, including biological species and involves the use of free flow isolectric focusing (FF-IEF) devices capable of establishing a pH gradient in a gradient orientation that is perpendicular to the direction of fluid flow. In one embodiment a channel ( | 03-17-2011 |
| 20110100821 | MINERAL OIL FREE ISOELECTRIC FOCUSING APPARATUS FOR IMMOBILIZED PH GRADIENT STRIPS - A protective sheath for use with an immobilized pH gradient (IPG) strip in an isoelectric focusing (IEF) process is generally disclosed. The protective sheath can inhibit dehydration of the IPG strip during use through loss of water to the atmosphere and/or migration of water due to electroosmotic flow without the use of mineral oil or another water-immiscible liquid. The protective sheath can generally be configured in a tube-like shape having an inner cavity. The protective sheath can define a top surface, a bottom surface, two side surfaces, a sealed end, and an open end. The top surface can have at least 2 openings, which can be covered by pull-tabs. | 05-05-2011 |
| 20110174624 | Methods and Apparatus for Carrier-Free Deflection Electrophoresis - The invention relates to a method and apparatus for carrier-free deflection electrophoresis, in which a separating media and a sample to be examined flow through a separating chamber between a pair of electrodes in a series of reversing bulk fluid flow along the direction of the electrodes, thereby separating the sample into zones which are to be collected into fractions for analysis or further processing. Among other things, the apparatus and method enable high-resolution separation of particles that can be performed in miniaturized chambers in electrophoresis modes including isoelectric focusing, zone electrophoresis, and isotachophoresis. | 07-21-2011 |
| 20110303542 | Use of Additives for Enhancing Droplet Operations - The invention relates to a droplet actuator with a substrate comprising electrodes arranged for conducting droplet operations on a droplet operations surface of the substrate; a filler fluid phase in contact with the droplet operations surface at least partially surrounding a droplet phase comprising a droplet arranged on one or more of the electrodes, the droplet comprising: (i) a target substance susceptible to loss from the droplet phase into the filler fluid phase; and (ii) an additive which reduces loss of the target substance to the filler fluid phase relative to a corresponding droplet not comprising the additive. The invention also relates to various compositions and methods. | 12-15-2011 |
| 20120043210 | Apparatus for Purifying Molecules - The invention provides devices for trapping and collecting separated biomolecules following a separation step. The invention also provides methods of using the devices to trap and collect separated biomolecules. | 02-23-2012 |
| 20120138469 | MICROFLUIDIC DEVICES FOR TRANSVERSE ELECTROPHORESIS AND ISOELECTRIC FOCUSING - Devices and methods are provided for separation of particles of a first selected electrophoretic mobility or isoelectric point from a fluid comprising particles of at least one other selected electrophoretic mobility or isoelectric point. The devices comprise a microchannel; electrodes to either side of the microchannel for applying a selected voltage to produce an electrical field across the microchannel orthogonal to the length of the microchannel; and outlets in said microchannel placed to receive outlet portions of the fluid containing enhanced concentrations of each type of particle. The devices may be used for particle detection, quantification, separation, mixing, dilution and concentration; to release, separate and detect interior particles of cells or organisms, and to separate particles such as proteins and microorganisms from biological fluids such as blood; or to separate and detect airborne contaminants such as bacterial warfare agents from air. | 06-07-2012 |
| 20120145548 | METHODS AND DEVICES OF SEPARATING MOLECULAR ANALYTES - A method of separating a mixture of a plurality of molecular analytes having different isoelectric points (pIs). The method comprises placing a solution containing a mixture of a plurality of molecular analytes in a separation volume, generating a pH profile having a plurality of pH zones across an axis of the separation volume, and adjusting a profile of the pH profile to induce a migration of a first molecular analyte along the axis apart from a second molecular analyte. The first and second molecular analytes having different pIs. | 06-14-2012 |
| 20130126356 | SIMULTANEOUS PURIFICATION OF CELL COMPONENTS - Methods and devices for purification of different cell components from the same sample are provided. | 05-23-2013 |
| 20130134042 | FFE Media and FFE Methods Comprising Volatile Separation Media - The present invention provides methods and separation media for separating analytes of interest via free flow electrophoresis (FFE) using volatile buffer systems. The separation media provided herein allow a convenient separation of the analytes by electrophoresis, and offer the additional advantage that the buffer compounds and the solvent can be easily and residue-free removed after the electrophoretic separation step. Furthermore, methods for mass spectrometric analysis of analytes comprising an FFE method and kits for carrying out FFE separations with volatile buffer systems are also provided. Preferably, the volatile buffer system is TRIS acetate. | 05-30-2013 |
| 20130153422 | STOPPED-FLOW, MICRO-FLUIDIC DEVICE AND METHOD FOR THE CHARGE-BASED SEPARATION OF COMPLEX ANALYTE MIXTURES - The present inventions relate to micro-fluidic devices and related methods of fractionating samples of analytes, such as peptides or proteins, according to their isoelectric points. The disclosed micro-fluidic devices and related methods provide a fractionation sufficient to enhance the performance of immunochemistry and/or tandem liquid chromatography-mass spectrometry workflows. Such methods and devices are capable of fractionating complex samples in a short time, using a small amount of sample, do not require high voltages and are further characterized by their high degree of reproducibility and ease of use. | 06-20-2013 |
| 20140166485 | ELECTROADSORPTION AND CHARGE BASED BIOMOLECULE SEPARATION AND DETECTION IN POROUS SENSORS - Electroadsorption and charged based biomolecule separation, concentration and detection with porous biosensors. In preferred embodiments, a potential is applied to a porous electrode to separate and concentrate molecules from solution. The bimolecular analytes are captured by the porous electrode itself, the same electrode that is used to generate the electric field for electroadsorption. In additional preferred embodiments, pH of the solution is adjusted to separate and concentrate biomolecules. Setting the pH equal to the protein isoelectric point was determined by the inventors to maximize concentration of biomolecules into the porous biosensor. The methods include simultaneously optically detecting charged molecules captured by the porous electrode. Methods of the invention are benign to biomolecules of interest, which are demonstrated to retain a high percentage of their activity after being released from the biosensor. Methods of the invention provide label-free detection. Advantageously, small voltages and ultrasmall volumes of solution are used in methods of the invention. | 06-19-2014 |
| 20150144491 | SOLID-LIQUID SEPARATION METHOD - To separate solid matter contained in a liquid phase in a membrane separation tank | 05-28-2015 |
