Class / Patent application number | Description | Number of patent applications / Date published |
204461000 | With analysis or detailed detection | 63 |
20080251380 | Medium for enhanced staining of single strand nucleic acids in electrophoresis - A medium is provided, being adapted to be applied in electrophoretic separation of nucleic acids. The medium comprises a staining reagent adapted to stain nucleic acids. Said medium further comprises a urea derivative, adapted to interact with said staining reagent and thereby providing an enhanced staining of said nucleic acids. Further, an electrophoresis device is provided, adapted to perform electrophoretic separation of nucleic acids, comprising the before medium and comprising electrodes for applying an electrical field across the medium. A method to perform electrophoresis using the before electrophoresis device and medium is provided. | 10-16-2008 |
20080257737 | ELECTROPHORESIS METHOD, ELECTROPHORESIS MODULE AND ELECTROPHORESIS APPARATUS - Disclosed is a technique for electrophoresis analysis, capable of eliminating the need for labeling a sample with a fluorescent or radioactive material. An electrophoresis cell is formed with a plurality of electrophoretic paths arranged in parallel relation to each other. Each of the electrophoretic paths has an upper end serving as a sample injection end, and a lower end received in a sample receiver. Further, each of the electrophoretic paths has a plurality of microchannels each extending uniformly from a position apart from the upper end by a predetermined distance to the lower end. The microchannels serve as a diffraction grating for diffracting light which irradiates a surface of the electrophoresis cell in a direction perpendicular to the surface, to produce diffracted light. | 10-23-2008 |
20080289964 | Assays for diagnosis and therapeutics employing similarities and differences in blood serum concentrations of 3 forms of complement C3c and related protein biomarkers between amyotrophic lateral sclerosis and Parkinson's disease - The invention relates to assays for diagnosis, drug targeting and therapeutic response monitoring, of patients with Parkinson's disease (PD), with PD-Like disorders that express symptoms like PD, age-matched controls (Normal), Patients with Amyotrophic Lateral Sclerosis (ALS), with ALS-Like disorders that express symptoms like ALS, and age-matched controls (Normal). The method is based on the use of 2-dimensional (2D) gel electrophoresis to separate the complex mixture of proteins found in blood serum, the quantitation of a group of identified biomarkers, and the biostatistical analysis of the concentration of the identified biomarkers. | 11-27-2008 |
20090045060 | METHOD FOR ANALYZING PROTEIN - A method for analyzing proteins with the use of electrophoresis is provided, which makes it possible to rapidly and conveniently analyze a great variety of proteins with high sensitivity. The method for analyzing a protein in a sample comprises setting a sample in a carrier for electrophoresis, performing electrophoresis for the sample using a buffer for electrophoresis in which a labeling compound represented by formula I: | 02-19-2009 |
20090071829 | RADIO FREQUENCY IDENTIFIERS FOR USE IN BIOLOGICAL SCIENCE - Provided herein are biological research methods, kits, and products that utilize radio frequency identifier technology. | 03-19-2009 |
20090090628 | NUCLEIC ACID DERIVATIVES AND METHODS OF USE - The objective of the invention is to provide nucleic acid derivatives with designated compounds introduced specifically into the base of nucleic acids. A DNA or RNA, or a nucleic acid molecular weight marker, wherein a designated compound (R), which is selected from any one of dyes, fluorescent dyes, RI labeled substances, and compounds capable of linking specifically to other compounds, is introduced at the N−3 position of a thymine base or uracil base in which (R′) at Position 1 is other than hydrogen atom. | 04-09-2009 |
20090095628 | CLEAVABLE SURFACTANTS - The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques. | 04-16-2009 |
20090101506 | METHOD, SPECIFIC PRIMERS AND KIT FOR THE DETECTION OF NEISSERIA MENINGITIDIS - The current invention refers to a | 04-23-2009 |
20090107841 | Two-dimensional strandness-and length-dependent separation of nucleic acid fragments - A method is provided for separating single- and double-stranded nucleic acid molecules based on their strandness and length. The method is based on novel two-dimensional gel electrophoresis techniques comprises loading a sample of nucleic acid molecules in a gel electrophoresis apparatus and electrophoresing in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that populations of single- and double-stranded nucleic acids are separated, said first and second electrophoresis conditions being different, such that in one dimension electrophoresis allows separation of the sample molecules based on strandness and length, and in the other dimension electrophoresis allows separation based substantially on length, wherein said difference is established with a chemical agent and/or physical parameter affecting the strandness-dependent electrophoresis migration rate of nucleic acids. | 04-30-2009 |
20090127115 | Method and Devices For Imaging a Sample - The invention relates to methods and devices for imaging a sample, in particular to methods and devices for imaging electrophoretic gels which have been used to separate biological molecules such as proteins or nucleic acids. The invention overcomes the problems associated with interference due to Newton's Rings and chemical toxicity experienced with conventional imaging systems. | 05-21-2009 |
20090127116 | Thermoresponsive microparticle composite hydrogels for electrophoresis - Disclosed are thermoresponsive microparticle composite hydrogels comprising poly(N-isopropyl acrylamide) and polyacrylamide, and methods regarding their manufacture and their use. The present invention provides in one aspect a thermoresponsive microparticle hydrogel, wherein the matrix morphology is controllably and selectively altered by incorporation of thermoresponsive nano/micro-particles. The particles are preferably poly(N-isopropyl acrylamide) particles. The present invention also provides methods of making and using such hydrogels. | 05-21-2009 |
20090134028 | Method of Estimating Effect of Test Chemical on Living Organisms - A method of predicting an effect of a test chemical on living organisms, which comprises:
| 05-28-2009 |
20090145758 | DEGRADABLE POLYACRYLAMIDE GEL - A degradable polyacrylamide gel for analyzing or separating at least one macromolecule in a sample using electrophoresis includes a polyacrylamide cross-linked with at least one degradable cross-linker having a ketal or acetal group with the formula (I), wherein R | 06-11-2009 |
20090145759 | Methods, Cassettes, Gels and Apparatuses for Isolation and Collection of Biomolecules from Electrophoresis Gels - Electrophoresis systems, assemblies, cassettes and methods for easily, and more effectively and efficiently, isolating a biomolecule band from an electrophoretic gel are provided. The methods use an electrophoresis cassette with at least one loading well and at lest one collection well. A sample containing the biomolecule of interest is placed into at least one loading well and buffer or water is placed in at lest one collection well. An electric field is then applied to drive migration and separation of the sample into different component bands within the gel. When the component of interest is located within at least one collection well, the electric field is terminated and the buffer or water in the collection well is removed, thereby isolating and collecting the sample component of interest. | 06-11-2009 |
20090152116 | ELECTROPHORETIC ANALYSIS OF MOLECULES USING IMMOBILIZED PROBES - Methods of detecting target molecules using electrophoresis and media containing immobilized capture are described. | 06-18-2009 |
20090178926 | Sharply Resolving Labeled Protein Molecular Weight Standards - Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced. | 07-16-2009 |
20090188796 | Method For Identifying At Risk Cardiovascular Disease Patients - The invention provides a data base of LDL, I, IIa, IIb, IIIa, IIb, IVa, IVb and HDL2a, HDL2b, HDL 3a, HDL 3b and HDL 3c together with patient data such as HDL-C, LDL-C, Apo A, ApoB, Lp(a) and patient personal data useful for treatment, diagnosing, and monitoring cardiovascular disease. The data base contains the LDL and HDL subfraction data in quantitative mg/dl values and permits deriving relationship amongst the LDL and HDL values and cardiovascular disease. Quantitative data permits more effective treatment and monitoring of cardiovascular disease. For example, quantitative differences in LDL and HDL subclass levels can determine the need for more or less aggressive treatment. The data base which includes patient events, procedures, interventions which is correlated to LDL and HDl quantitative subclass data permits development of personalized patient treatment plans and monitoring the effectiveness of such treatment. Thus, LDL and HDL quantitative subfraction data can be used to more effectively treat and monitor cardiovascular disease. | 07-30-2009 |
20090211908 | Devices and methods for detecting and quantitating nucleic acids using size-separation of amplicons - Devices and methods are described for detecting and quantifying nucleic acids using a sealed system that minimizes contamination. In particular, provided herein are devices for and methods using nucleic acid amplification that permit multiple sampling of an amplification reaction mixture and quantitation and identification of amplicons during the course of an amplification reaction. Methods involving the transfer of samples from an amplification reaction mixture into a separation network, separation of nucleic acids based on size, and identification and quantitation of nucleic acids are disclosed. | 08-27-2009 |
20090236225 | MICRORNA DETECTION - Provided herein are methods for detection of miRNA in a sample. In certain embodiments, the sample comprises RNA and is derived from a cell or tissue. The methods of detection employ a competitor molecule to allow for detection of an miRNA in the presence of an anti-miRNA oligonucleotide. | 09-24-2009 |
20090272649 | Method for study, determination or evaluation - A method for studying, determining or evaluating a pharmacological action of a test substance, the method including subjecting the brain tissue of an SART stressed animal administered with the test substance to an expression proteome analysis, where expression changes of NSF (N-ethylmaleimide sensitive fusion protein), which is or is not modified after translation, in the SART stressed animal administered with the test substance as compared with an SART stressed animal to which a test substance is not administered is used as an index. | 11-05-2009 |
20090277791 | METHOD FOR SEPARATION AND IDENTIFICATION OF BIOMOLECULES USING UNCONVENTIONAL GEL ELECTROPHORESIS AND DETECTION OF SINGLE NANOPARTICLE PROBES - A method for identifying and separating of target biomolecules is disclosed. The method involves contacting a sample with a nanoparticle probe that is capable of specifically binding a target biomolecule. The complex formed by the nanoparticle target biomolecule is separated by electrophoresis using a non-conventional gel composed of a synthetically cross-linked polymer and a gel stabilizer. The nanoparticle probe:target biomolecule complex is detected, thereby identifying the target biomolecule. In certain examples, the nanoparticle probe emits a signal to identify its location. Particular examples of nanoparticle probes are semiconductor nanocrystals, such as quantum dots, which fluoresce at a particular wavelength. Also disclosed is a method of substantially sealing and/or increasing the transparency of a membrane. Such a method includes applying a film to a membrane. Sealing the membrane allows the detection of single nanoparticles. | 11-12-2009 |
20090294288 | High-sensitivity proteolysis assay - The present invention includes a highly sensitive method for detecting the presence of proteases in a sample which are present at very low levels. | 12-03-2009 |
20100000865 | METHOD AND REAGENT FOR PROTEIN ANALYSIS - A convenient, rapid, and highly sensitive protein detection method is provided. According to the present invention, an easily water-soluble compound of formula I is provided: | 01-07-2010 |
20100006437 | DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING COUPLED LIGASE DETECTION AND POLYMERASE CHAIN REACTIONS - The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences. | 01-14-2010 |
20100012495 | Set of Novel Oligonucleotide Primers and the Method for the Detection of Aspergillus Ochraceux Thereby - The present invention relates to a process for the detection of | 01-21-2010 |
20100025245 | SELECTIVE FLUORESCENT LABELING OF S-NITROSOTHIOLS (S-FLOS): A NOVEL METHOD FOR STUDYING S-NITROSYLATION - A method and kit for selectively labeling S-nitrosylated cysteines in proteins with a fluorescent tag. The method offers femtomolar sensitivity for the detection, quantification, in situ visualization, and a means for site-specific identification of S-nitrosylation events. | 02-04-2010 |
20100032295 | Continuous film electrophoresis - The present invention relates to systems for continuous film electrophoresis and processes for parallel DNA sequencing. | 02-11-2010 |
20100044229 | Electrophoretically Enhanced Detection of Analytes on a Solid Support - The present embodiments provide systems, kits and methods suitable for performing dry or substantially dry electro-blotting analyses on immobilized protein or nucleic acid samples. Electro-blotting performed according to the presently described embodiments may include a step whereby detection of one or more immobilized proteins or nucleic acids is electrophoretically accelerated. Methods for performing electro-blotting of immobilized proteins or nucleic acids may include applying an electric voltage to one or more reagents typically used in protein or nucleic acid blotting procedure. The one or more reagents may be absorbed on a suitable carrier matrix. Electro-blotting performed in accordance with the systems and methods described herein may be performed under substantially dry conditions (i.e., with little or no aqueous buffers). | 02-25-2010 |
20100059376 | NOVEL ANALYTICAL METHOD FOR PROTEIN - Disclosed is a method of analyzing a protein, comprising the steps of: (A) labeling small amounts of protein samples with CyDyes having different fluorescent properties, respectively; (B) mixing 50-100 μg of each of the protein samples, labeled in step (A), with 1-5 mg of each of one or more of the unlabeled protein samples, and subjecting the mixture to electrophoresis; (C) subjecting the electrophoresed gel to fluorescence analysis to detect a difference in protein distribution between the protein samples; and (D) excising spot(s), showing the difference in protein distribution, from the gel, and isolating and identifying from the spots a protein, which is different in protein distribution between the protein samples. According to the present invention, a protein from a gel can be identified by MALDI-TOF, while simultaneously analyzing the gel qualitatively and quantitatively using a difference in the fluorescence of CyDyes with very high sensitivity. | 03-11-2010 |
20100084270 | Integrated Microfluidic Component for Purifying Analyte Molecules and Purification Method - The present invention relates to a method for purifying analyte molecules and in particular to a component of this type in which a separation section is used for separating analyte molecules and other constituents of a sample, and in which provision is made of at least one sample chamber for receiving a sample containing the analyte molecules and at least one collecting chamber for receiving the purified analyte molecules. According to the invention, the microfluidic component has at least one integrated receptor device for detecting the presence and/or the concentration of the purified analyte molecules. In accordance with one advantageous development of the present invention, the separation section is formed by an electrophoretic gel filtration section. | 04-08-2010 |
20100089753 | FLUORESCENT DETECTION OF PROTEINS IN POLYACRYLAMIDE GELS - The mechanism of the UV light-induced reaction between the indole moiety of tryptophan and chloroform, and the structure of the modified tryptophan and polypeptides including such modified tryptophan residues. The excited indole moiety, which is formed upon UV light irradiation, emits a solvated electron which initiates a series of events that yield fluorescent derivatives that have CHO group covalently bound to the indole moiety. These derivatives are herein referred to as formyltryptophan, and are relatively stable. Similar reactions are observed when 5-hydroxytryptophan, 5-fluorotryptophan, or N-methylindolacetate are used in place of tryptophan, or when other haloalkanes, such as trichloracetic acid, trichlorethanol, trichlorethane, bromoform, and iodoactetate are used in place of chloroform. The derivatives can be used in a variety of applications in fluorescence spectroscopy, and for nuclear magnetic resonance, X-ray crystallography, infra-red spectroscopy, circular dicroism and mass spectroscopy. Additionally, the UV light-induced reaction between the indole moiety of tryptophan and haloalkanes can be used to prepare derivatives of tryptophan for chemical cross-linking studies of proteins and peptides. | 04-15-2010 |
20100089754 | METHOD OF MEASURING MICROPARTICLES HAVING NUCLEIC ACID AND APPARATUS THEREFOR - To provide a system by which evaluation of circumstances of contamination by microparticles having nucleic acid can be performed rapidly and accurately. The theme is achieved by a system for measuring microparticles that includes: (1) a microparticle adhesion step of adhering the microparticles having nucleic acid to a microparticle adhesion member; (2) a membrane breakage step of breaking membranes of the adhered microparticles by electrical discharge; (3) an electrophoresis step of electrophoresing the microparticles in a thickness direction of a gel to make the nucleic acid in the microparticles migrate from a negative electrode side toward a positive electrode side and adhere the nucleic acid on a surface of a nucleic acid detection member; and (4) a nucleic acid measurement step of fluorescently staining the surface of the nucleic acid detection member to measure a concentration of the nucleic acid. | 04-15-2010 |
20100140090 | Gel suspension apparatus - A method and apparatus for expeditiously carrying out the preparation and imaging steps of the electrophoresis process. The apparatus of the invention includes a novel supporting surface comprising TEFLON® AF that acts as a multi-function gel supporting surface which minimizes the probability of damaging the gel during transfer and provides a supporting surface that has an index of refraction lower than that of water and that of water based gel. When the TEFLON® AF supporting surface is used to support the gel, or other mostly aqueous, fluorescent sample, the surface becomes a planar, aqueous core waveguide which traps the excitation light thereby increasing the efficiency of excitation. | 06-10-2010 |
20100155245 | Thrombospondin Fragments and Binding Agents in the Detection, Diagnosis and Evaluation of Cancer - The invention relates to thrombospondin fragments found in plasma, their use or use of portions thereof in diagnostic methods, as method calibrators, method indicators, and as immunogens, and as analytes for methods with substantial clinical utility; and their detection in plasma or other bodily fluids for purpose of diagnostic methods, especially for cancer. | 06-24-2010 |
20100264029 | PLATE FOR MASS SPECTROMETRY, PROCESS FOR PREPARING THE SAME AND USE THEREOF - The present invention provides a plate for mass spectrometry comprising a support and a PVDF-containing coating (that is, a PVDF-deposited thin layer) thereon, and a method of preparing a plate for mass spectrometry, which comprises coating the support surface with PVDF. The present invention also provides a method of analyte identification comprising subjecting an analyte-containing sample to gel electrophoresis to separate the analyte, blotting the separated analyte from the gel to the above-described plate for mass spectrometry, and subjecting the plate to mass spectrometry, whereby the transferred analyte is analyzed. | 10-21-2010 |
20100288638 | SEPARATION OF BIOMOLECULES - A method and apparatus for performing separation of molecules, in particular biomolecules such as nucleic acids and peptides, are disclosed. The method comprises separating molecules by a first characteristic along a linear dimension of a channel, and separating the molecules by a second characteristic along the same linear dimension, with the rust and second separations being carried out within at least partially overlapping sections of the channel. The two separations may then be combined to give a virtual two-dimensional separation which is carried out in a single dimensional real space. The method may also include detecting molecules during the second separation, preferably using an intrinsic characteristic of the molecules for example UV absorbance. | 11-18-2010 |
20100294664 | Assay for ALS and ALS-like disorders - The invention relates to an assay for discriminating between amyotrophic lateral sclerosis (ALS) patients and patients with ALS-like disorders that express symptoms like ALS. The method is based on the use of 2-dimensional (2D) gel electrophoresis to separate the complex mixture of proteins found in blood serum, the quantitation of a group of identified biomarkers, and the biostatistical analysis of the concentration of the identified biomarkers to differentiate patients having ALS from patients having other ALS-like disorders. | 11-25-2010 |
20100314251 | Diagnosis of Parkinson's disease - A method of diagnosing Parkinson's disease uses the abnormal concentrations of a group of 21 blood serum protein biomarkers. The concentration of the 21 protein biomarkers is assessed in a patient's serum by quantitative two-dimensional polyacrylamide gel electrophoresis. | 12-16-2010 |
20100326827 | METHOD AND APPARATUS FOR ISOLATING AND PURIFYING BIO-MOLECULES - Electrophoresis systems, apparatus, and methods for isolating or purifying a bio-molecule are provided. The method includes using an electrophoresis apparatus for isolating or purifying a bio-molecule, wherein the electrophoresis apparatus includes: a separation tube including a first section for containing buffer and a second section including an electrophoresis gel, and a collection tube including a solid phase, wherein the solid phase fully covers the cross-sectional area of the collection tube, and the collection tube is detachably connected to an end adjacent to the second section of the separation tube. | 12-30-2010 |
20100326828 | RAPID ELECTROPHORESIS BINDING METHOD AND RELATED KITS AND COMPOSITIONS - An improved staining method is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods and related kits are also described. | 12-30-2010 |
20110042213 | GEL ELECTROPHORESIS, IMAGING, AND ANALYSIS METHODS, DEVICES, SYSTEMS, AND MATERIALS - The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing. | 02-24-2011 |
20110147218 | SOLUBLE CADHERIN 17 FOR THE DIAGNOSIS AND RISK STRATIFICATION OF CANCER AND TUMOR OF THE GASTROINTESTINAL TRACT - The invention relates to a method for the diagnosis and risk stratification of a tumor or cancer of the gastrointestinal tract, particularly colon tumor or colon cancer, wherein a determination is made on at least one patient using the novel soluble cadherin 17 biomarker—a proteolytic cleavage product of cadherin 17 having SEQ ID No. 1 or SEQ ID No. 2—or partial peptides and fragments thereof. | 06-23-2011 |
20110253536 | METHOD FOR INCREASING MEASUREMENT PRECISION OF TWO-DIMENSIONAL PROTEIN - A method for increasing measurement precision of two-dimensional protein electrophoresis is provided, in which the electrical conductivity of a protein sample under test is measured for calculating the electrical energy required to enable salt and protein focusing. The method is characterized by a set of equations for calculating the electrical energy required respectively for protein focusing and for electrophoresis of salts in the protein sample, wherein the calculation is based on the electrical conductivity of the salts, the protein weight, a pH-gradient gel strip length, and a pH range. Thus, different protein samples can be supplied with the appropriate amounts of electrical energy for isoelectric focusing, so as to produce the optimal protein focusing effects and ensure that the focusing of protein in a gel will not be adversely affected by an otherwise insufficient or excessive supply of electrical energy. | 10-20-2011 |
20110259744 | Sensors for biomolecular detection and cell classification - A sensor device is provided for detecting an analyte in a sample in which an analyte is bound to a detection reagent to form a bound complex. The device comprises (a) a sample ( | 10-27-2011 |
20120000781 | Electro-blotting Devices, Systems, and Kits and Methods for Their use - The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer. | 01-05-2012 |
20130020199 | METHODS, CASSETTES, GELS AND APPARATUSES FOR ISOLATION AND COLLECTION OF BIOMOLECULES FROM ELECTROPHORESIS GELS - Electrophoresis systems, assemblies, cassettes and methods for easily, and more effectively and efficiently, isolating a biomolecule band from an electrophoretic gel are provided. The methods use an electrophoresis cassette with at least one loading well and at least one collection well. A sample containing the biomolecule of interest is placed into at least one loading well and buffer or water is placed in at least one collection well. An electric field is then applied to drive migration and separation of the sample into different component bands within the gel. When the component of interest is located within at least one collection well, the electric field is terminated and the buffer or water in the collection well is removed, thereby isolating and collecting the sample component of interest. | 01-24-2013 |
20130032481 | SAMPLE CARRIER AND/OR SAMPLE CARRIER PROCESSING APPARATUS - A sample processing apparatus includes a sample carrier receiving region configured to receive a sample carrier. The sample carrier includes at least one sample channel carrying at least one sample, at least one agent chamber carrying at least one agent to be moved to the at least one sample channel to facilitate processing of the at least one sample, and the at least one agent chamber includes at least one chamber cover covering at least one opening of the at least one agent chamber, inhibiting flow of the at least one agent from the at least one agent chamber to the at least one sample channel. The sample processing apparatus further includes a chamber opener configured to facilitate opening the at least one chamber cover. The sample processing apparatus further includes a fluid mover that moves the agent out of the at least one agent chamber after the at least one chamber cover is opened and into the at least one sample channel. | 02-07-2013 |
20130105320 | SLIDE HOLDER ASSEMBLY FOR COMET ASSAY | 05-02-2013 |
20130199930 | METHOD AND SYSTEM FOR DOCUMENTING AN ELECTROPHORESIS GEL - Disclosed in this specification is a high capacity compact gel documentation system for documenting different types of electrophoresis gels or other translucent objects using ultraviolet light. The system includes a base with a scanning surface having a transparent bottom surface. A light source is connected to a conveying mechanism, disposed below the transparent bottom surface, to move the light source over the length of the transparent bottom surface. An image capture device receives a reflected image and provides it to a microprocessor. Moreover, separate interchangeable filters allow for documentation of UV and white light gel captures, alleviating the need for separate transilluminators and hoods with filters and cameras. | 08-08-2013 |
20130319864 | COMPOSITION AND METHOD FOR GEL ELECTROPHORESIS WITH IN-SITU CALIBRATION - The invention relates to, among other things, a method for performing electrophoresis with in-situ calibration. The method includes combining a volume of a test sample with a volume or quantity of a calibrating sample to form a final volume, where the volume of the calibrating sample includes a known concentration of a calibrator and the final volume includes a known ratio of test sample to calibrating sample. The method also includes depositing a loading fraction in a receiving well of an electrophoretic gel, in which the loading fraction is a fraction of the final volume, and separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane. The method also includes detecting the calibrator and separated components of the test sample within the common separation lane and measuring the level of the calibrator and separated components of the test sample based on the detecting, thereby performing electrophoresis with in-situ calibration. | 12-05-2013 |
20140042027 | MODIFIED ELECTRODE BUFFERS FOR STAIN-FREE PROTEIN DETECTION IN ELECTROPHORESIS - Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products. | 02-13-2014 |
20140076726 | Detection of Immobilized Nucleic Acid - The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. | 03-20-2014 |
20140202859 | AUTOMATED SIZE SELECTION OF NUCLEIC ACIDS - Apparatus and methods for size selecting nucleic acid molecules having wide range of applications including the production of DNA libraries for sequencing technologies. An automated high throughput system for size selection of multiple nucleic acid samples that uses imaging technique to detect the progress of a target fraction and feedback from the imaging to control electrophoresis. Predictive algorithms for timed nucleic acid extractions are generated to provide size selected nucleic acid molecules of required size ranges. | 07-24-2014 |
20140311909 | PROTEIN STANDARD - Disclosed herein is a protein standard for gel electrophoresis. The standard may be detected using multiple modalities. These modalities include, for example, observation of color or fluorescence arising from dyes, porphyrins, or haloalkylated tryptophan residues covalently linked to proteins of the standard; or detection of fluorescence or chemiluminescence arising from antibodies or other binding partners bound to proteins of the standard through polypeptide epitopes or affinity tags. The protein standard comprises multiple protein sets, each set corresponding to a different gel band, and proteins within a set may be detectable with one or more modalities. | 10-23-2014 |
20150047980 | METHODS OF PERFORMING A SIZING ANALYSIS USING A CORRECTED SIZING LADDER - The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination. | 02-19-2015 |
20150060279 | ELECTROPHORETICALLY ENHANCED DETECTION OF ANALYTES ON A SOLID SUPPORT - The present embodiments provide systems, kits and methods suitable for performing dry or substantially dry electro-blotting analyses on immobilized protein or nucleic acid samples. Electro-blotting performed according to the presently described embodiments may include a step whereby detection of one or more immobilized proteins or nucleic acids is electrophoretically accelerated. Methods for performing electro-blotting of immobilized proteins or nucleic acids may include applying an electric voltage to one or more reagents typically used in protein or nucleic acid blotting procedure. The one or more reagents may be absorbed on a suitable carrier matrix. Electro-blotting performed in accordance with the systems and methods described herein may be performed under substantially dry conditions (i.e., with little or no aqueous buffers). | 03-05-2015 |
20150060280 | METHOD FOR CLASSIFYING AND DISCRIMINATING JATROPHA LINES USING RETROTRANSPOSON AS A MARKER - lines can be classified and discriminated by a method including the steps of: conducting a nucleic acid amplification reaction using a primer set including (i) adjacent forward primer and (ii) adjacent reverse primer, and (iii) LTR forward primer or LTR reverse primer, or (iv) RTP forward primer or RTP reverse primer, wherein DNA prepared from | 03-05-2015 |
20150083594 | ELECTROPHORESIS CONTROLLERS, SENSORS, AND METHODS FOR CONTROLLING ELECTROPHORESIS PROCESSES - An electrophoresis controller for use with an electrophoresis apparatus having a gel matrix disposed between electrodes for separation of particles along with a tracking dye. The electrophoresis controller includes a sensor system and a controller. The sensor system includes a support, a light emitter, and a light receiver. The support includes a first portion positionable on a first side of the gel matrix and a second portion positionable adjacent a second side of the gel matrix. The light emitter is positioned on the first portion of the support for emitting light onto one side of the gel matrix. The light receiver is positioned on the second portion of the support adjacent to the other side of the gel matrix for receiving light from the light source as it is passing through the gel matrix. At least one of the light emitter and the light receiver includes a light guide having a first end and a second end. The first end is positioned on the support and facing the gel matrix, and the second end is remote from the sensor system. The controller is operably connected to the sensor for monitoring a change in the light from the illuminated gel matrix due to migration of the tracking dye into the illuminated gel matrix and received by the light receiver. | 03-26-2015 |
20150144490 | Methods and Compositions for Preparing Biological Specimens for Microscopic Analysis - Methods and compositions are provided for preparing a biological specimen for microscopic analysis. These methods find many uses, for example in medicine and research, e.g., to diagnose or monitor disease or graft transplantation, to study healthy or diseased tissue, to screen candidate agents for toxicity and efficacy in disease modification. Also provided are reagents, devices, kits and systems thereof that find use in practicing the subject methods. | 05-28-2015 |
20150308978 | DIELECTRIC ELECTROLYTE MEASUREMENT DEVICE - A system, device and apparatus for measuring electrolytes, where an electrical charge is applied to a measurement portion to draw ions from a liquid to a gel-solution via at least one electric field. The gel-solution containing the extracted ions is excited with light of a predetermined wavelength from an emitter. A receiver detects the illumination of the ions as a result of the excited gel-solution, and a processor converts the detected intensities of the illumination to a biologically useful value representing ionic concentration. | 10-29-2015 |
20160061774 | GEL ELECTROPHORESIS, IMAGING, AND ANALYSIS METHODS, DEVICES, SYSTEMS, AND MATERIALS - The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing. | 03-03-2016 |
20160123924 | PARTICLE SIZE DISTRIBUTION MEASUREMENTS OF PARTICLES AND DROPLETS USING OPTICAL GEL ELECTROPHORESIS - A device for measuring size distributions of particles and droplets includes a gel electrophoresis component that has a gel chamber that is suitable to receive a gel in which at least one of particles or droplets propagate in a liquid medium during operation; an illumination source arranged to illuminate said at least one of particles or droplets such that the at least one of particles or droplets absorbs, scatters or emits light; an imaging device configured to obtain image data from the absorbed, scattered, or emitted light from the at least one of particles or droplets while the at least one of particles or droplets propagate through the gel; and a computing device configured to receive and process the image data to provide information concerning a size distribution of the at least one of particles or droplets. | 05-05-2016 |
20160153911 | INSTANT VIEW OF PROTEIN BANDS | 06-02-2016 |