Entries |
Document | Title | Date |
20080217177 | Capillary electrophoresis apparatus - There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed. | 09-11-2008 |
20080264792 | Apparatus for Protein Separation Using Capillary Isoelectric Focusing-Hollow Fiber Flow Field Flow Fractionation and Method Thereof - Disclosed herein is an apparatus for the separation of proteins, comprising a capillary isoelectric focusing unit ( | 10-30-2008 |
20090032400 | Use of support materials in capillary electrochromatography - Use of a support material for capillary electrochromatography (CEC), characterized in that the support material has a porous design and a surface which consists of an outer surface and a pore surface, wherein the outer surface has regions of different derivatization and/or functionality from that of the pore surface. | 02-05-2009 |
20090038943 | METHOD OF ELECTROPHORETIC ANALYSIS OF MULTICOMPONENT SOLUTIONS AND DEVICE FOR PERFORMING THE SAME - The present invention relates to analytical chemistry, more particularly, to methods of electrophoretic analysis, and can be utilized to analysis of multicomponent solutions. | 02-12-2009 |
20090045059 | Method and apparatus for measuring membrane potential of red blood cells using electrophoretic analysis - The present invention provides a method and apparatus for measuring the membrane potential of red blood cells using electrophoretic analysis that analyzes red blood cells whose membrane potentials are reversed due to red blood cell agglutination. | 02-19-2009 |
20090071827 | METHOD AND APPARATUS TO REDUCE FALSE POSITIVE AND FALSE NEGATIVE IDENTIFICATIONS OF COMPOUNDS - A method, computer program medium, and system for analyzing a protein sample that obtains a mass spectrum of the derived peptides, and determines from the mass spectrum a first set of peptide identifications for the peptides. In the method, computer program medium, and system, incorrect identifications can be filtered from the first set of peptide identifications by removal from the first set those peptide identifications ascertained to be false identifications; and the filtered first set can be filtered to generate a second set of the peptide identifications corresponding to the protein sample. | 03-19-2009 |
20090084680 | SAMPLE STACKING METHOD USING ON-LINE AUTOMATIC SOLID PHASE EXTRACTION COUPLED TO NONAQUEOUS CAPILLARY ELECTROPHORESIS AND INTERFACE STRUCTURE BETWEEN SOLID-PHASE PRECONCENTRATION CARTRIDGE AND CAPILLARY THEREFOR - A sample stacking method using on-line automatic solid phase extraction coupled to nonaqueous capillary electrophoresis, and an interface structure between a solid-phase preconcentration cartridge and a capillary therefor. The sample analysis method using solid phase extraction coupled to nonaqueous capillary electrophoresis by connecting a solid-phase preconcentration cartridge with a capillary includes: extracting a sample on a solid phase; injecting an elution solvent at an outlet terminal of the capillary, the elution solution desorbing analytes adsorbed onto a solid-phase material of a solid-phase preconcentration cartridge; and injecting a nonaqueous buffer solution from the outlet terminal of the capillary to push the elution solvent to the solid-phase material. | 04-02-2009 |
20090173631 | Single Cell Analysis of Membrane Molecules - Interrogation of surface receptors of individual cells within a population of cell types in a single mixture is described. Each cell comprises, bound to target surface receptors on the cell, a collection of binding compositions, each comprising a distinct molecular tag. A continuous flow of cell medium containing the cells is pneumatically transported through a channel in a separation device, toward a cleavage/release zone, where specific tags correlating to each binding composition, and thus to each corresponding cell surface receptor, are released from the binding compositions on the cell surface. The method is effective to produce a separately detectable collection of tag signals for each cell. | 07-09-2009 |
20090205959 | AUTOMATIC SAMPLE PROCESSING METHOD AND AUTOMATIC SAMPLE PROCESSING APPARATUS FOR LID-SEALED MICROCHIPS FOR BIOANALYSIS - The present invention provides a technique for automating the operation to peel off and remove a lid seal part adhered and fixed to the top face of a substrate part constituting a lid-sealed “microchip” after subjecting a sample solution to be analyzed to electrophoretic separation. After subjecting sample solution to be analyzed to desired electrophoretic separation the by utilizing a channel formed in the lid-sealed microchip, the electrophoretically separated liquid sample held in the channel undergoes freezing of the aqueous solvent contained and then, while the whole electrophoretically separated liquid sample remains sustained in the frozen state, an end of the lid seal part used for sealing the top face of channel formed in the substrate part is lifted up at a predetermined speed so as to peel and remove it from the substrate part under a condition of maintaining a bend of a predetermined radius of curvature. | 08-20-2009 |
20090205960 | DEVICE AND METHOD FOR DETECTING ARTICLES WITH PIPETTE AND NANOPORE - A device and a method for detecting and/or characterizing particles are shown. The device includes at least one nanopore, a voltage source for generating an electric potential difference between the two sides of the at least one nanopore in order to generate an ion current through the nanopore when the nanopore is surrounded by an electrolyte, and a measuring instrument adapted for recording a change in the impedance of the at least one nanopore in respect of the ion current when one or more particles that are to be detected and are present in the electrolyte pass(es) through the at least one nanopore. The device also includes a pipette with an end portion in which an opening is formed. A flow of the particles that are to be detected from outside the pipette is generated through the opening and through the nanopore, and the pipette is moved relative to a sample. | 08-20-2009 |
20090321259 | Portable Preparation, Analysis, and Detection Apparatus for Nucleic Acid Processing - The present teachings comprise a device and method for lysing and/or purifying biological sample. The device can comprise a cartridge having a chamber containing a biological sample receiving region, a plurality of electrodes, and one or more sieving matrices. The electrodes can be configured to lyse the biological sample through the production of a pulsed electrical field. The electrodes can also be configured to heat lyse the biological sample. The electrodes can also be configured to electrophoretically move the biological sample through one or more sieving matrices. A portion of the sample can be isolated on a membrane. The portion of the sample isolated on the membrane can be amplified and detected. A portion of the sample can be isolated in a collection area present in the cartridge. The portion of the sample isolated in the collection area can be removed from the cartridge. | 12-31-2009 |
20100078325 | DEVICES AND METHODS FOR DETERMINING THE LENGTH OF BIOPOLYMERS AND DISTANCES BETWEEN PROBES BOUND THERETO - Devices and methods for detecting the length of analytes and/or sequencing analytes are provided in which two or more electrical signals are obtained as an analyte traverses a fluidic channel. Detection of the relative position of probes hybridized to a biopolymer and/or the length of the analyte (e.g., a biopolymer) does not rely on the absolute time between detection events of a given electrical signal to determine a distance associated with the biopolymer. Instead, multiple signals are obtained (e.g., as functions of time) corresponding to a plurality of detector volumes at known locations along a fluidic channel through which the biopolymer passes, and the distances are determined from the multiple signals. | 04-01-2010 |
20100108512 | ELECTROPHORETIC CHIP AND METHOD OF USING THE SAME | 05-06-2010 |
20100108513 | CAPILLARY ELECTROPHORESIS USING CLEAR COATED CAPILLARY TUBES - A capillary tube having a hard, optically clear external coating or cladding. In one embodiment, the external clear coating comprises hard-fluoropolymer. The hard-fluoropolymer coating bonds to the fused silica glass, providing higher strength and superior static fatigue performance resulting in vastly improved bending flexibility. The thin hard-fluoropolymer coating of capillaries provides higher initial tensile strength, longer lifetime (resistance to stress corrosion or static fatigue) and superior ability to transmit excitation light and emitted light directly through the coating for fluorescence based detection. | 05-06-2010 |
20100116658 | ANALYSER AND METHOD FOR DETERMINING THE RELATIVE IMPORTANCE OF FRACTIONS OF BIOLOGICAL MIXTURES - An analyser and method for determining the relative importance of fractions of biological mixtures projects data obtained from at least two mixtures with different physiological conditions by chromatographic or mass spectrometric measurement into a second attribute space using a projection technique such as principal component analysis. The projected data is then filtered using a feature selection method such as ReliefF, before being projected back to the first attribute space using a reversion of the projection technique. This back-projected data is then filtered using another feature selection method such as ReliefF before being output in a human-readable form. This technique improves the clarity of the data by removing components relating to noise or systematic error and therefore makes it easier to determine which fractions of biological mixtures are most important for distinguishing between the different biological mixtures and identifying the physiochemical attributes that correspond to the difference in physiological conditions. The technique is useful in medical diagnostics, quality control and basic biomedical science. | 05-13-2010 |
20100116659 | Multidimensional Separations Employing an Array of Electrophoresis Channels - The present invention relates to a method for separating components of a sample. The method includes obtaining a first separation of the sample components along a first dimension wherein the sample components are at least partially resolved, wherein the first separation can be performed in the absence of an electric field applied to the first dimension. An electric field is used to obtain a second separation of the sample components along a second dimension comprising a plurality of substantially isolated volumes. An intensity-time data record is obtained from each of the isolated volumes, the intensity-time data records containing peaks, each peak being indicative of a migration time. The migration time of a first peak is normalized with respect to a migration time of at least a second peak to correct for migration time differences between the isolated volumes. | 05-13-2010 |
20100116660 | Electrophoresis Chip, Electrophoresis Apparatus, and Method for Analyzing Sample by Capillary Electrophoresis - An electrophoresis chip that can be small and simple and that can analyze a sample with high accuracy is provided. The electrophoresis chip includes an upper substrate | 05-13-2010 |
20100126861 | Polypeptide Markers for the Diagnosis and Evaluation of Vascular Diseases - A process for diagnosing vascular diseases (VD), comprising the step of determining the presence or absence of at least one polypeptide marker in a sample, wherein said polypeptide marker is selected from markers 1 to 526, which are characterized by values for the molecular masses and migration times (CE times). | 05-27-2010 |
20100147688 | 2-IN-1 BIOCHIP - This invention relates to the Capillary Electrophoresis (CE) Biochip. This chip uses the technology of CE combined with conductivity detection to determine: blood Electrolytes (EL) of K3 Na, Ca & Li using “EL Biochip” and to monitor water quality using “L Biochip”. Our in use technology enables to develop a small chip, reliable, easy to use, inexpensive and capable for rapid whole blood testing anywhere. Once this technique is developed, a generic system is obtained, and multitude of ions can be tested at once on the same device. Our chips are using same hardware (layout) and can be optimized to test other ions. The “2-in-1 Biochip” is a multi application versatile system, having two chips : “EL Biochip” : a point-of-care blood analysis chip, for diagnosis & therapeutic follow-up. “L-Biochip”: an on-site environmental monitoring liquids chip, to monitor water quality & guarding against water born diseases outbreaks. | 06-17-2010 |
20100155243 | System and Method For the Separation of Analytes - A separation module operates to fractionate or separate an analyte into fractions according to pI, i.e., pI bands, utilizing capillary isoelectric focusing (“CIEF”) within a first microchannel. The fractions are stacked to form plugs, the number of which is determined by a number of parallel second microchannels integrally connected to the first microchannel, into which the fractions are directed according to the buffer characteristics found in each of the individual microchannels. Within the microchannels the plugs are separated into proteins according to a different chemical property, i.e., “m/z,” utilizing capillary electrophoresis (“CE”). | 06-24-2010 |
20100181196 | DETERMINATION OF THE HYDRODYNAMIC RADII OF THE CONSTITUENTS OF AN ADMIXTURE BY MEANS OF ANALYSIS OF A TAYLOR DISPERSION CARRIED OUT FOLLOWING A SEPARATION BY MEANS OF CAPILLARY ELECTROPHORESIS - A method for determining the hydrodynamic radius for the constituents of an admixture, includes: (A) by separating capillary electrophoresis, the constituents of the admixture, leaving them within the capillary; (B) at one of the ends of the capillary obtained in this manner, containing, in different zones, the separated constituents, a detectable marker is injected in the region of a detection device which is placed at the side of the other end of the capillary; (C) a pressure difference is induced between the ends of the capillary in order to cause the various constituents separated in step (A) and finally the marker to migrate towards the outlet of the capillary; and (D) by analyzing the Taylor dispersion produced in step (C), the hydrodynamic radius is determined for each of the constituents, based on the detection time of the marker and the elution profile of each of the constituents. | 07-22-2010 |
20100187112 | NANOFLUIDIC PRECONCENTRATION DEVICE IN AN OPEN ENVIRONMENT - This invention provides a device and methods for increasing the concentration of a charged species in solution, wherein the solution containing the concentrated species is exposed to the environment. Such solution can be formed on a surface or on a tip of a measurement device. The open-environment concentration technique overcomes the disadvantages of in-channel concentration devices, especially by eliminating flow-induced delivery processes that lead to concentration losses. Combined with direct contact dispensing, methods of this invention can be used for various applications such as immunoassay and MALDI-MS. | 07-29-2010 |
20100213063 | ANALYSIS - Analysis methods and apparatus are provided for inspecting a channel, such as a capillary electrophoresis channel, in a device. Configuration and alignment systems are provided, together with optical systems and temperature control. | 08-26-2010 |
20100243450 | NON-EQUILIBRIUM CAPILLARY ELECTROPHORESIS OF EQUILIBRIUM MIXTURES (NECEEM)-BASED METHODS FOR DRUG AND DIAGNOSTIC DEVELOPMENT - The invention discloses a Non-Equilibrium Capillary Electrophoresis of Equilibrium Mixtures (NECEEM) method and NECEEM-based practical applications. The NECEEM method is a homogeneous technique, which, in contrast to heterogeneous methods, does not require affixing molecules to a solid substrate. The method of the invention facilitates 3 practical applications. In the first application, the method allows the finding of kinetic and thermodynamic parameters of complex formation. It advantageously allows for revealing two parameters, the equilibrium dissociation constant, K | 09-30-2010 |
20100243451 | PCR METHODS FOR CHARACTERIZING THE 5' UNTRANSLATED REGION OF THE FMR1 AND FMR2 GENES - This disclosure relates to methods of determining the presence and position of AGG or interruptor elements within a trinucleotide (for example, CGG) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the CGG repeat region, and resolving the products to produce a representation of product size and abundance. | 09-30-2010 |
20100270157 | ISOTACHOPHORESIS - Problems to be Solved | 10-28-2010 |
20110011740 | CAPILLARY IMMUNOASSAY SYSTEMS AND METHODS - An automated assay system is described with stations for placement of materials to be used in an assay of materials inside capillaries and an automated gripper for manipulating capillaries. The system includes a separation/detection station where reactions inside the capillaries take place and photoemissions from the capillary reactions are detected. The photoemissions from the capillaries may be displayed as line graphs or in columns of a pseudo-gel image resembling the familiar Western gel blot. An automated control system has a user interface by which an operator can select a run protocol and define the locations of samples and reagents to be used in the protocol run. Following the setup the control system will cause the automated system to execute the protocol, then display the results in a selected display format. | 01-20-2011 |
20110089034 | DIELECTROPHORESIS APPARATUS INCLUDING CONCENTRATION GRADIENT GENERATING UNIT, METHOD OF SEPARATING MATERIAL USING THE SAME, AND METHOD OF SCREENING CONDITION FOR SEPARATING MATERIAL - A dielectrophoresis (DEP) apparatus including a concentration gradient generating unit, a method of separating a target material in a sample solution using the DEP apparatus, and a method of screening the optimum condition for separating a target material are provided. | 04-21-2011 |
20110120868 | Nanopore and Carbon Nanotube Based DNA Sequencer and a Serial Recognition Sequencer - The present invention is directed to systems, devices and methods for identifying biopolymers, such as strands of DNA, as they pass through a constriction such as a carbon nanotube nanopore. More particularly, the invention is directed to such systems, devices and methods in which a newly translocated portion of the biopolymer forms a temporary electrical circuit between the nanotube nanopore and a second electrode, which may also be a nanotube. Further, the invention is directed to such systems, devices and methods in which the constriction is provided with a functionalized unit which, together with a newly translocated portion of the biopolymer, forms a temporary electrical circuit that can be used to characterize that portion of the biopolymer. | 05-26-2011 |
20110120869 | METHOD FOR MULTIPLEXED CAPILLARY ELECTROPHORESIS SIGNAL CROSS-TALK CORRECTION - The present invention provides a simple method to correct cross-talk, after the data have been generated. Adjacent signals are simply subtracted from the original observed signal with a repeating process. The data processing is stopped when a predefined condition is met. By this technique, cross-talk can be reduced from >5% to less than 0.1%. And as an additional advantage, this method provides a way to correct the cross-talk without the need to know which peaks are caused by the adjacent capillary signal. | 05-26-2011 |
20110147217 | METHOD AND APPARATUS FOR CANCER SCREENING - A method and apparatus for detection of pteridine levels in a biological sample using CE-LIF which is useful for early cancer screening involving fully oxidizing pteridine compounds in a sample such as a urine sample, subjecting to CE-LIF to assess compound concentration, and compare to expected levels in for healthy or cancer-bearing patients. | 06-23-2011 |
20110174622 | DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT - The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid. | 07-21-2011 |
20110198225 | AMPLIFIED ELECTROKINETIC FLUID PUMPING SWITCHING AND DESALTING - The present invention provides a device and methods of use thereof for desalting a solution. The methods, inter-alia, make use of a device comprising microchannels, which are linked to conduits, whereby induction of an electric field in the conduit results in the formation of a space charge layer within the microchannel. The space charge layer provides an energy barrier for salt ions and generates an ion depletion zone proximal to the linkage region between the microchannel and the conduit. The method thus enables the removal of salt ions from the region proximal to the conduit and their accumulation in a region distant from the conduit, within the microchannel. | 08-18-2011 |
20110210000 | Isoelectric particles and uses therefore - A plurality of particles of from about 5 nm to 100 μm possessing predetermined isoelectric points in the pH range of from about 2.5 to 11 is used in a method of detection of a plurality of analytes, wherein the isoelectric particles of each isoelectric point further contain as label and a member of a binding pair capable of interacting with a selected analyte. The particles that form specific binding pairs are recovered and separated by isoelectric focusing followed by the detection of the labels associated with the particles. A flow cytometer may be used as a detector of the isoelectric particles. | 09-01-2011 |
20110214991 | MICROFLUIDIC DEVICE AND METHOD OF DETERMINING NUCLEOTIDE SEQUENCE OF TARGET NUCLEIC ACID USING THE SAME - A microfluidic device includes at least one first channel and at least one second channel or chamber which is connected to the first channel via a nanopore in a fluid communication manner, and a method of determining a nucleotide sequence of a target nucleic acid by using the same. Accordingly, the nucleotide sequence of the target nucleic acid may be efficiently determined. | 09-08-2011 |
20110253535 | NON-GEL BASED TWO-DIMENSIONAL PROTEIN SEPARATION MULTI-CHANNEL DEVICES - Provided is a multi-channel apparatus for non-gel based two-dimensional protein separation. One or more flat channels are arranged in parallel and have an isoelectric focusing section for primarily separating proteins from protein samples according to isoelectric point (pI) and a flow field-flow fractionation section for secondarily separating the primarily separated proteins according to molecular weight, thereby making it possible to simultaneously separate the proteins in multiple channels, to increase a protein separation speed, and to overcome limitation to sample injection due to an increase in channel volume. The apparatus can separate the proteins according to pI and molecular weight, be safe from denaturation of the protein in the process of protein separation, automatically remove an ampholyte used for pI-based separation, and separately detecting the separated proteins to identify the proteins using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS). | 10-20-2011 |
20110259743 | METHOD FOR DETERMINATION OF OXIDATIVE STRESS - Provided is a biomarker that enables easy and rapid detection of oxidative stress on a living organism and enables prevention of tissue damage or cell necrosis by drug administration, and which is a powerful marker for the study of toxicity and pharmacokinetics of various agents. Oxidative stress is determined by measuring blood concentration of ophthalmic acid, which is a substance that varies in blood depending on the variation of reduced glutathione (GSH) concentration in a biological sample with the use of an analyzer such as a capillary electrophoresis-mass spectrometer. Further, an anti-oxidative stress agent is screened by administering an anti-oxidative stress candidate agent to a non-human animal under oxidative stress conditions, measuring blood concentration of ophthalmic acid, and evaluating the degree of decrease in the ophthalmic acid concentration. | 10-27-2011 |
20110290648 | Capillary Electrophoresis Device - This invention provides a capillary electrophoresis device in which capillaries are thermally regulated on a thermally responsive electrical path attached to an electrically insulating circuit board. This invention also provides an optical scanner useful for scanning an array of capillaries. A laser, optical detector and optical selector are in an arrangement that allows the optical detector to selectively detect an optical signal from any one or more of the plurality of electrophoresis capillaries. | 12-01-2011 |
20110308950 | METHOD FOR DETERMINING BIOPOLYMER USING NANOPORE, AND SYSTEM AND KIT THEREFOR - Although analysis can be very quickly conducted at a low cost by a method for measuring a biopolymer using a nanopore, the accuracy of distinguishing the individual monopolymers constituting the biopolymer is low. To both ends of a biopolymer through a nanopore, molecules which are larger than the nanopore are attached and then the biopolymer is reciprocated by an external force to thereby perform repeated measurements. | 12-22-2011 |
20120043208 | APPARATUS AND METHODS FOR HIGH THROUGHPUT BIOMOLECULE SEPARATION AND ANALYSIS - A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel “tagless” strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of | 02-23-2012 |
20120085645 | DIGITAL MICROFLUIDIC LIQUID-LIQUID EXTRACTION DEVICE AND METHOD OF USE THEREOF - Methods and devices for liquid-liquid extraction using digital microfluidic arrays are provided. A polar droplet is transported to a separation region containing a substantially non-polar solvent, where non-polar impurities may be extracted from the polar droplet while maintaining a distinct phase separation. In a preferred embodiment, biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in a separation step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with high precision and recovery. | 04-12-2012 |
20120090996 | MICRO FLUIDIC OPTIC DESIGN - A DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first separation channel for electrophoretic separation of DNA fragments in a first sample. Further, the DNA analyzer includes a first optical device. The first optical device includes an illuminating path and a detecting path. The illuminating path directs a first input light beam received from a light source to a first separation channel of the microfluidic chip. The first input light beam causes fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light. The detecting path collects and directs the first fluorescent light to a first plurality of optical fibers. Further, the DNA analyzer includes a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light. | 04-19-2012 |
20120125775 | CHEMICAL MODIFICATION OF PROTEINS FOR THEIR MORE ACCURATE MOLECULAR-WEIGHT DETERMINATION BY ELECTROPHORESIS - Disclosed herein is a composition and method for sample preparation of proteins for their size separation by electrophoresis, suitable for molecular-weight determination of proteins in the range between about 14,000 and 500,000. In an embodiment, proteins, particularly those exhibiting biased migration, are modified to change their intrinsic charge, or carbohydrate component to improve accuracy of their molecular weights as determined by electrophoretic size separation via their interaction with ionic surfactants. In a preferred embodiment, the proteins are carbamylated with potassium cyanate and their carbohydrate components are oxidized with sodium periodate. | 05-24-2012 |
20120152742 | Massively Parallel 2-Dimensional Capillary Electrophoresis - This invention discloses a highly efficient method, system and apparatus for nucleic acid analysis, including sequencing (both automated re-sequencing and de-novo sequencing). The system is capable of sequencing DNA sizes ranging from fragments to mammalian size genomes having mouse draft quality at a much reduced cost. The system comprises a massive parallel capillary electrophoretic separation using two-dimensional monolith multi-capillary arrays (2D-MMCA). Sequence identification can be performed using fluorescent or otherwise labeled dideoxynucleotide-terminated DNA extension product generated by gel matrix-, or beads-, or substrate tethered-, or otherwise immobilized colonies of single template molecules. Cost reduction is a significant advantage over currently known methods because of: (i) using massively parallel sub-nanoliter volume reactions; and (ii) employing 2D-MMCAs that increase the throughput of the CE separation and detection by at least two orders of magnitude compared to the commercial high-throughput DNA machines. | 06-21-2012 |
20120160681 | NANOPORE-BASED SINGLE DNA MOLECULE CHARACTERIZATION, IDENTIFICATION AND ISOLATION USING SPEED BUMPS - The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump. | 06-28-2012 |
20120241322 | Analysis Apparatus and Analysis Method - An analysis apparatus is an apparatus that performs electrophoresis using a microchip provided with a channel. The analysis apparatus includes a cooling unit (an electron cooling element and a driving circuit) that cools the microchip, a voltage application unit (electrodes and a power supply circuit) that applies voltage to a buffer solution filled in the channel of the microchip, an optical analysis unit (a light source, a light receiving element, and an analysis unit) that conducts, through the microchip, optical analysis of a sample introduced in the channel, and a control unit that controls the cooling unit, the voltage application unit, and the optical analysis unit. The control unit causes the cooling unit to start cooling the microchip, and after the microchip has been cooled, causes the voltage application unit and the optical analysis unit to operate. | 09-27-2012 |
20120273353 | HIGH THROUGHPUT MOBILITY SHIFT - The present invention provides novel methods for performing pulsed field mobility shift assays in microfluidic devices. In particular, the methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction. | 11-01-2012 |
20120292184 | MICROBIAL IDENTIFICATION AND MANIPULATION OF NANOSCALE BIOMOLECULES - A method of microbial identification is disclosed. The method includes the steps of assembling dielectrophoretic particles modified with specific DNA probes on a surface thereof in a continuous fluid at a predetermined location in a microchannel to form a particle assembly by a negative dielectrophoretic force and a hydrodynamic force provided by the continuous fluid, narrowing gaps between the dielectrophoretic particles of the particle assembly to enhance the electric field in the gaps between the dielectrophoretic particles, injecting a fluid containing target DNAs of a target microbe into the microchannel at a predetermined flow rate to move the target DNAs toward the particle assembly and generating a positive dielectrophoretic force by the enhanced electric field to attract the target DNAs toward the dielectrophoretic particles of the particle assembly for hybridization with the DNA probes. The present invention also discloses a method of manipulation of nanoscale biomolecules. | 11-22-2012 |
20130001083 | METHOD AND APPARATUS FOR ANALYSIS AND SORTING OF POLYNUCLEOTIDES BASED ON SIZE - The invention relates to a microfabricated device and methods of using the device for analyzing and sorting polynucleotide molecules by size. | 01-03-2013 |
20130015066 | CONTROL OF PARTICLE FLOW IN AN APERTUREAANM Van Der Voorn; Johannes AdrianusAACI ChristchurchAACO NZAAGP Van Der Voorn; Johannes Adrianus Christchurch NZ - The flow of particles ( | 01-17-2013 |
20130026038 | DEVICES AND METHODS FOR DETERMINING THE LENGTH OF BIOPOLYMERS AND DISTANCES BETWEEN PROBES BOUND THERETO - Devices and methods for detecting the length of analytes, and/or sequencing analytes are provided in which two or more electrical signals are obtained as an analyte traverses a fluidic channel. Detection of the relative position of probes hybridized to a biopolymer and/or the length of the analyte (e.g., a biopolymer) does not rely on the absolute time between detection events of a given electrical signal to determine a distance associated with the biopolymer. Instead, multiple signals are obtained as functions of time) corresponding to a plurality of detector volumes at known locations along a fluidic channel through which the biopolymer passes, and the distances are determined from the multiple signals. | 01-31-2013 |
20130043131 | TRAPPING MOLECULAR SEGMENTS IN NANO-GAPS - A molecule trapping method includes forming a fluid bridge between a first reservoir and a second reservoir, translocating a molecule from the first reservoir to the second reservoir through the fluid bridge, detecting when a segment of the molecule is in the fluid bridge, breaking the fluid bridge and forming an a gap between the first and the second reservoirs, thereby trapping a segment of the molecule in the gap and making measurements on the segment of the molecule. | 02-21-2013 |
20130075258 | Method for Automated High Throughput Identification of Carbohydrates and Carbohydrate Mixture Composition Patterns as well as Systems Therefore - The present invention relates to methods for the identification of compounds in carbohydrate mixture compositions as well as the determination of carbohydrate mixture composition patterns, based on e.g. orthogonal cross determining migration time (indices) using capillary gel electrophoresis-laser induced fluorescence and identifying said carbohydrate components based on comparing said migration time (indices) with standard migration time (indices) from a database which data are preferably also orthogonal cross determined. In a further aspect, the present invention relates to a method for carbohydrate mixture composition pattern profiling, like glycosylation pattern profiling using capillary gel electrophoresis-laser induced fluorescence (CGE-LIF). In another aspect, the present invention refers to a system for an automated determination and/or identification of carbohydrates and/or carbohydrate mixture composition patterns (e.g.: glycosylation patterns). Finally, the present invention relates to a database containing e.g. orthogonal cross normalized migration times and/or migration time indices of carbohydrates. | 03-28-2013 |
20130092539 | DROPLET-BASED NUCLEIC ACID AMPLIFICATION METHOD AND APPARATUS - Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets. | 04-18-2013 |
20130112559 | DEVICE AND METHOD FOR HANDLING DROPS - The invention relates to a microfluidic device and a method for handling at least one drop. | 05-09-2013 |
20130146458 | RAPID COMPACT ASSAY FOR PARENT RADIONUCLIDES IN GENERATORS - Disclosed embodiments assay for the presence of Ge-68, although disclosed embodiments can also be used for other radionuclide generators, with minimal adjustments. | 06-13-2013 |
20130180855 | CAPILLARY ELECTROPHORESIS METHOD FOR ANALYZING COLLAGEN - A capillary electrophoresis method for identification and analyzing collagen quantitatively, which is used to identify and quantify collagen in a sample, comprises the steps of: (a) dissolving a collagen-containing sample to form a sample solution; (b) preparing a capillary with an inner wall thereof having a positively-charged layer; (c) introducing the sample solution into the capillary filled with an analytical buffer solution; and (d) driving the sample solution to pass through the capillary. The method of the present invention does not need the purifying pre-treatment and cracking the collagen-containing sample but directly performs the capillary electrophoresis analysis of collagen. Therefore, the present invention can shorten the time for analyzing collagen quantitatively. | 07-18-2013 |
20130186757 | Nanopore and Carbon Nanotube Based Dna Sequencer and A Serial Recognition Elements - The present invention is directed to systems, devices and methods for identifying biopolymers, such as strands of DNA, as they pass through a constriction such as a carbon nanotube nanopore. More particularly, the invention is directed to such systems, devices and methods in which a newly translocated portion of the biopolymer forms a temporary electrical circuit between the nanotube nanopore and a second electrode, which may also be a nanotube. Further, the invention is directed to such systems, devices and methods in which the constriction is provided with a functionalized unit which, together with a newly translocated portion of the biopolymer, forms a temporary electrical circuit that can be used to characterize that portion of the biopolymer. | 07-25-2013 |
20130186758 | CURRENT-CARRYING NANOWIRE HAVING A NANOPORE FOR HIGH-SENSITIVITY DETECTION AND ANALYSIS OF BIOMOLECULES - Disclosed are devices that feature nanopores formed in graphene sheets, such as graphene ribbons. The graphene sheets include an edge irregularity, such as a zig-zag configuration, a chiral edge, or an armchair-configuration edge, which edges confer on the devices the ability to discriminate between different subunits of a macromolecule translocated through the nanpore. The disclosed devices and methods have application to DNA sequencing and analysis, among other applications. | 07-25-2013 |
20130213810 | Ruggedized Apparatus for Analysis of Nucleic Acid and Proteins - The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location. | 08-22-2013 |
20130248365 | METHOD FOR ISOTOPIC MEASUREMENT BY ICPMS - A method for isotope measurement of charged species contained in a solution to be analyzed, particularly charged species having an isobaric interference, has the following consecutive steps:
| 09-26-2013 |
20130264206 | BIOMOLECULE DETECTION APPARATUS INCLUDING PLURALITY OF ELECTRODES - A biomolecule detection apparatus comprising a nanopore device having a front surface and rear surface and including a nanopore having a nano-sized diameter; a reservoir disposed adjacent to a rear surface of the nanopore device; and a power supply unit comprising a first electrode disposed in a front of the nanopore device; a second electrode disposed inside the reservoir; and a third electrode disposed adjacent the nanopore and between the first electrode and the second electrode; as well as a method of using the biomolecule detection apparatus to detect a biomolecule in a sample. | 10-10-2013 |
20130264207 | DNA SEQUENCING BY SYNTHESIS USING MODIFIED NUCLEOTIDES AND NANOPORE DETECTION - This disclosure is related to a method of sequencing a single-stranded DNA using deoxynucleotide polyphosphate analogues and translocation of tags from incorporated deoxynucleotide polyphosphate analogues through a nanopore. | 10-10-2013 |
20130319861 | Outputting A Droplet Of Liquid Medium From A Device For Processing Micro-Objects In The Medium - A micro-fluidic device can include a processing mechanism for processing micro-objects in a liquid medium and an outputting mechanism for expressing from the device a droplet of the medium containing one or more of the micro-objects. The outputting mechanism can include an expressing mechanism having a reservoir for holding a quantity of the liquid medium and a striking mechanism for striking and compressing the expressing mechanism to express a droplet of the medium from the expressing mechanism. | 12-05-2013 |
20130319862 | LEAKAGE CURRENT SENSE CIRCUIT FOR ERROR DETECTION IN AN IMPROVED CAPILLARY ELECTROPHORESIS-ELECTROSPRAY IONIZATION- MASS SPECTROMETRY SYSTEM - Aspects of the present innovations relate to improved systems that may perform capillary electrophoresis (CE) and CE in conjunction with electrospray ionization (ESI) as an input to a mass spectrometry system (MS). Embodiments may use a current sense circuit at a high voltage output from an MS-ESI power supply in conjunction with additional elements to identify fault conditions associated with leakage current, to confirm the continuity of CE connections, and to provide improved system protection. | 12-05-2013 |
20130334047 | DEVICE FOR DETERMINING A MONOMER MOLECULE SEQUENCE OF A POLYMER COMPRISING DIFFERENT ELECTRODES AND USE THEREOF - Provided is a device for determining a monomer molecule sequence of a polymer including different electrodes, and a method of efficiently determining a monomer molecule sequence of a polymer. | 12-19-2013 |
20130341189 | Microchip for analyzing nucleic acid and method of nucleic acid analysis using the same - A method for nucleic acid analysis including injecting a slight amount of nucleic acid sample for analysis, characterized in that most of a nucleic acid sample which is not used, excluding the slight amount of the nucleic acid sample which is used for analysis, can be obtained as a pure product which is not contaminated with a fluorescent material, and a microchip for analyzing nucleic acid which enables such method for nucleic acid analysis, are provided. The method for nucleic acid analysis may analyze at least two different nucleic acid samples in a continuous manner by sequentially injecting the at least two different nucleic acid samples. | 12-26-2013 |
20140008225 | METHOD OF DETERMINING OR ESTIMATING NUCLEOTIDE SEQUENCE OF NUCLEIC ACID - A method of determining or estimating a nucleotide sequence of a nucleic acid by using a device with a nanopore. | 01-09-2014 |
20140021049 | MICROFLUIDIC SYSTEMS - A flow cell for a microfluidic device can include a chamber, first microfluidic entry and exit channels, second microfluidic entry and exit channels, a first electrode, and a second electrode. A microfluidic device can include a microfluidic channel, a laser to excite fluorescent material, and a detector to detect fluorescence emission. Methods of merging a droplet from an emulsion in to a second stream of fluid and of detecting a content of a droplet in a stream are further disclosed. | 01-23-2014 |
20140110258 | Integrated Affinity Microcolumns and Affinity Capillary Electrophoresis - Device and method for detecting the presence of known or unknown toxic agents in a fluid sample. Targets in the sample are bound to releasable receptors immobilized in a reaction region of a micro- or nano-fluidic device. The receptors are selected based on their affinity for classes of known toxic agents. The receptors are freed and the bound and unbound receptors separated based on differential electrokinetic mobilities while they travel to a detection device. | 04-24-2014 |
20140110259 | METHOD AND DEVICE FOR OPTICAL ANALYSIS OF BIOPOLYMER - A biopolymer optical analysis apparatus is provided with a biopolymer characteristic analysis chip including a solid substrate, nanopores provided in the substrate, and electrically conductive thin films on the substrate, at least portions of the thin films facing the nanopores, a light source and an irradiation optical system for producing Raman scattered lights from biopolymers entering the nanopores, and a detection device including a Raman scattered light collecting system, a separating component that splits collected light into transmitted light and reflected light, an image-forming optical system through which the split lights form images, and a two-dimensional detector for detecting the lights forming the images. External light from the light source and the irradiation optical system is applied to the characteristic analysis chip, and the detection device detects Raman scattered lights from biopolymers in the analysis chip. Characteristics of monomer units constituting the biopolymers are analyzed from the results of detection. | 04-24-2014 |
20140131204 | MOLECULAR ENTRAPMENT AND ENRICHMENT - Methods, structures, devices and systems are disclosed for rapid enrichment and mass transport of biomolecules (e.g., such as proteins) or other small molecules and particles using electrodeless dielectrophoresis (eDEP). In one aspect, a device to aggregate molecules includes a substrate that is electrically insulating, an electrically insulative material formed on the substrate and structured to form a channel to carry an electrically conducting fluid containing particles, a constriction structure formed of the electrically insulative material and located in the channel to narrow a channel dimension and forming an opening with a size in the nanometer range, and a circuit coupled to the substrate to apply an ac electric field and a dc bias electric field along the channel, in which the constriction structure is structured to magnify the applied ac electric field to produce forces that operate collectively to aggregate the particles. | 05-15-2014 |
20140158537 | MICROFLUIDIC METHODS OF ASSAYING MOLECULE SWITCHING AND DEVICES FOR PRACTICING THE SAME - Microfluidic methods of assaying molecule switching are provided. Aspects of the methods include microfluidically separating a sample containing the molecule of interest and then employing the resultant separation pattern to determine a switching characteristic of the molecule. Also provided are microfluidic devices, as well as systems and kits that include the devices, which find use in practicing embodiments of the methods. The methods, devices, systems and kits find use in a variety of different applications, such as analytical and diagnostic assays. | 06-12-2014 |
20140166484 | Systems and Methods for an Integrated Bio-Entity Manipulation and Processing Semiconductor Device - An integrated semiconductor device for manipulating and processing bio-entity samples is disclosed. The device includes a microfluidic channel that is coupled to fluidic control circuitry, a photosensor array coupled to sensor control circuitry, an optical component aligned with the photosensor array to manipulate a light signal before the light signal reaches the photosensor array, and a microfluidic grid coupled to the microfluidic channel and providing for transport of bio-entity sample droplets by electrowetting. The device further includes logic circuitry coupled to the fluidic control circuitry and the sensor control circuitry, with the fluidic control circuitry, the sensor control circuitry, and the logic circuitry being formed on a first substrate. | 06-19-2014 |
20140174927 | Nanopore Sensors for Biomolecular Characterization - Provided herein are methods and devices for characterizing a biomolecule parameter by a nanopore-containing membrane, and also methods for making devices that can be used in the methods and devices provided herein. The nanopore membrane is a multilayer stack of conducting layers and dielectric layers, wherein an embedded conducting layer or conducting layer gates provides well-controlled and measurable electric fields in and around the nanopore through which the biomolecule translocates. In an aspect, the conducting layer is graphene. | 06-26-2014 |
20140190830 | MICROFLUIDIC FEEDBACK USING IMPEDANCE DETECTION - Methods comprising measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode are disclosed. Computer readable mediums storing processor executable instructions for performing the method, and systems are also disclosed. The systems comprise a processor, memory and code stored in the memory that when executed cause the processor at least to: receive an output voltage signal, superimpose an excitation signal onto the output voltage signal to produce a superimposed signal, connect the superimposed signal to an electrode in a droplet actuator, suppress the output voltage signal, when detecting an impedance of the electrode, and measure the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode. | 07-10-2014 |
20140231254 | Method of Fabricating a Nanochannel System for DNA Sequencing and Nanoparticle Characterization - A process for fabricating a nanochannel system using a combination of microelectromechanical system (MEMS) microfabrication techniques, atomic force microscopy (AFM) nanolithography, and focused ion beam (FIB). The nanochannel system, fabricated on either a glass or silicon substrate, has channel heights and widths on the order of single to tens of nanometers. The channel length is in the micrometer range. The nanochannel system is equipped with embedded micro and nanoscale electrodes, positioned along the length of the nanochannel for electron tunneling based characterization of nanoscale particles in the channel. Anodic bonding is used to cap off the nanochannel with a cover chip. | 08-21-2014 |
20140238856 | NANOFLUIDIC DEVICES WITH INTEGRATED COMPONENTS FOR THE CONTROLLED CAPTURE, TRAPPING, AND TRANSPORT OF MACROMOLECULES AND RELATED METHODS OF ANALYSIS - Devices for controlling the capture, trapping, and transport of macromolecules include at least one fluidic transport nanochannel that intersects and is in fluid communication with at least one transverse nanochannel with (shallow) regions and/or with integrated transverse electrodes that enable fine control of molecule transport dynamics and facilitates analyses of interest, e.g., molecular identification, length determination, localized (probe) mapping and the like. | 08-28-2014 |
20140246317 | NANOPORE-BASED DETERMINATION OF PROTEIN CHARGE, SHAPE, VOLUME, ROTATIONAL DIFFUSION COEFFICIENT, AND DIPOLE MOMENT - Physical parameters of macromolecules are determined by measuring electrical current I over time for translocation events as the macromolecules in solution move between two liquid compartments that are separated by and fluidically coupled through a synthetic nanopore. Values of charge, volume, shape, rotational diffusion coefficient, ad dipole moment are derived from the measurements. | 09-04-2014 |
20140262783 | OPTICAL DETECTION FOR BIO-ENTITIES - An integrated semiconductor device for manipulating and processing bio-entity samples and methods are described. The device includes a lower substrate, at least one optical signal conduit disposed on the lower substrate, at least one cap bonding pad disposed on the lower substrate, a cap configured to form a capped area, and disposed on the at least one cap bonding pad, a microfluidic channel, wherein a first side of the microfluidic channel is formed on the lower substrate and a second side of the microfluidic channel is formed on the cap, a photosensor array coupled to sensor control circuitry, and logic circuitry coupled to the fluidic control circuitry, and the sensor control circuitry. | 09-18-2014 |
20140262784 | COUPLING METHOD - The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing. | 09-18-2014 |
20140299472 | Systems and Methods for an Integrated Bio-Entity Manipulation and Processing Semiconductor Device - An integrated semiconductor device for manipulating and processing bio-entity samples is disclosed. The device includes a microfluidic channel that is coupled to fluidic control circuitry, a photosensor array coupled to sensor control circuitry, an optical component aligned with the photosensor array to manipulate a light signal before the light signal reaches the photosensor array, and a microfluidic grid coupled to the microfluidic channel and providing for transport of bio-entity sample droplets by electrowetting. The device further includes logic circuitry coupled to the fluidic control circuitry and the sensor control circuitry, with the fluidic control circuitry, the sensor control circuitry, and the logic circuitry being formed on a first substrate. | 10-09-2014 |
20140305799 | ELECTRONIC CONTROL OF FLUIDIC SPECIES - Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one aspect, the invention relates to systems and methods for making droplets of fluid surrounded by a liquid, using, for example, electric fields, mechanical alterations, the addition of an intervening fluid, etc. | 10-16-2014 |
20140311907 | SYSTEMS AND METHODS FOR ASSAY OF BIO-CONTAMINANTS IN WATER - A method of determining water quality of a water sample, comprising exposing the water sample to a test cell system; generating at least one profile of ensuing changes in activities of transcription factors in said test cell system in response to said exposing; and determining from the generated at least one profile the water quality of the water sample. Computer systems and kits for carrying out the water quality determination of water specimens are also described, in which water quality can be readily and accurately determined by transcription factor activity analysis. | 10-23-2014 |
20140318966 | SILICON OXIDE NANOPORE WETTING AND STABILIZATION BY MOLECULAR COATING - A nanopore device includes a multi-layer structure comprising a surface defining an aperture extending through the multi-layer structure, wherein at least the surface comprising a minimal diameter comprises a monosilane functionalized silicon dioxide having a silicon-oxygen-silicon bond, the monosilane functionalized silicon dioxide having the following structure: | 10-30-2014 |
20140367259 | METHOD FOR IDENTIFYING A TARGET POLYMER - A method for identifying a target polymer ( | 12-18-2014 |
20150008124 | DEVICES AND METHODS FOR DETERMINING THE LENGTH OF BIOPOLYMERS AND DISTANCES BETWEEN PROBES BOUND THERETO - Devices and methods for detecting the length of analytes and/or sequencing analytes are provided in which two or more electrical signals are obtained as an analyte traverses a fluidic channel. Detection of the relative position of probes hybridized to a biopolymer and/or the length of the analyte (e.g., a biopolymer) does not rely on the absolute time between detection events of a given electrical signal to determine a distance associated with the biopolymer. Instead, multiple signals are obtained (e.g., as functions of time) corresponding to a plurality of detector volumes at known locations along a fluidic channel through which the biopolymer passes, and the distances are determined from the multiple signals. | 01-08-2015 |
20150021183 | Method and Apparatus for the Analysis and Identification of Molecules - An apparatus and method for performing analysis and identification of molecules have been presented. In one embodiment, a portable molecule analyzer includes a sample input/output connection to receive a sample, a nanopore-based sequencing chip to perform analysis on the sample substantially in real-time, and an output interface to output result of the analysis. | 01-22-2015 |
20150101931 | GRAPHENE SUPPORTED ARTIFICIAL MEMBRANES AND USES THEREOF - The invention features the use of graphene, a one atom thick planar sheet of bonded carbon atoms, in the formation of artificial lipid membranes. The invention also features the use of these membranes to detect the properties of polymers (e.g., the sequence of a nucleic acid) and identify transmembrane protein-interacting compounds. | 04-16-2015 |
20150122651 | Method and Fluidic Microsystem for Generating Droplets Dispersed in a Continuous Phase - A method of generating droplets of a dispersed phase fluid in a continuous phase fluid includes flowing the dispersed phase fluid and the continuous phase fluid to a channel junction of at least one dispersed phase channel and at least one continuous phase channel, applying at least one alternating voltage to at least two electrodes so that an alternating electric field is created at the channel junction, and generating the droplets of the dispersed phase fluid in the continuous phase fluid flowing in an output channel of the channel junction, wherein the dispersed phase fluid and the continuous phase fluid are electrically insulated from the at least two electrodes. Furthermore, a microfluidic device is described, which is configured for generating droplets of a dispersed phase fluid in a continuous phase fluid. | 05-07-2015 |
20150315638 | PRESENTING STRETCHED STRANDS OF SINGLE STRAND DNA FOR SEQUENCING DOUBLE STRAND DNA - A mechanism is provided for presenting single strands of a double strand molecule to a membrane. The double strand molecule is driven to a first side of the membrane by an electric field. The membrane has a first and second nanopore spaced apart by a nanopore separation distance. The first strand of the double strand molecule is captured in the first nanopore when driven to the first side of the membrane. The second strand is captured in the second nanopore by having the nanopore separation distance between the first nanopore and the second nanopore corresponding to a strand separation distance between the first and second strands, and/or by having captured the first strand to limit diffusion of the second strand. The first and second strands respectively in the first and second nanopores are individually stretched, by the first and second strands recombining on the second side of the membrane. | 11-05-2015 |
20150316504 | PRESENTING STRETCHED STRANDS OF SINGLE STRAND DNA FOR SEQUENCING DOUBLE STRAND DNA - A mechanism is provided for presenting single strands of a double strand molecule to a membrane. The double strand molecule is driven to a first side of the membrane by an electric field. The membrane has a first and second nanopore spaced apart by a nanopore separation distance. The first strand of the double strand molecule is captured in the first nanopore when driven to the first side of the membrane. The second strand is captured in the second nanopore by having the nanopore separation distance between the first nanopore and the second nanopore corresponding to a strand separation distance between the first and second strands, and/or by having captured the first strand to limit diffusion of the second strand. The first and second strands respectively in the first and second nanopores are individually stretched, by the first and second strands recombining on the second side of the membrane. | 11-05-2015 |
20150316529 | SYSTEM AND METHOD FOR REAL-TIME ANALYSIS OF MOLECULAR SEQUENCES USING NANOCHANNELS - The present invention relates to a system for analyzing molecular sequences, which is capable of decoding unit molecules constituting various biopolymers on a real-time basis using nanochannels. A control electrode serves to control the unit molecules passing along the channel such that the velocity of movement, arrangement, and directivity of the unit molecules can be rendered uniform. Particularly, at least four probe electrodes are separately formed in the case of decoding ss-DNA base molecules. Each probe electrode is coated with four different types of DNA base molecules to maximize detection efficiency through the interaction with complementary base molecules moving along the inside of the channel. | 11-05-2015 |
20150323495 | NON-AQUEOUS MICROCHIP ELECTROPHORESIS FOR CHARACTERIZATION OF LIPID BIOMARKERS - The invention provides devices and methods for the detection of hydrophobic biomarkers using 3D microchip capillary electrophoresis having a non-aqueous solvent system. Hydrophobic biomarkers can be placed in a microcapillary microchannel and electrokinetically injected into a second microcapillary microchannel through a nanocapillary array membrane. The hydrophobic biomarkers can then be separated and analyzed via mass spectrometry. Certain hydrophobic biomarkers can indicate a particular disease state. | 11-12-2015 |
20150338347 | GLYCAN PROFILING UTILIZING CAPILLARY ELECTROPHORESIS - A method for glycan profiling by capillary electrophoresis (CE), and a CE system for glycan analysis (N-Glycan). The CE system uses integrated dual optical fibers for both radiation excitation and emission detection. The CE system is configured for performing a two-color detection for data analysis. A single radiation excitation source is used to excite two emission fluorophores or dyes in the sample solution to be analyzed. One emission dye is to tag the sample and the other dye is used to provide a reference marker (e.g., a Dextran Ladder) for the sample run. Two detectors (e.g., photomultipler tubes (PMTs)) are applied to simultaneously detect the fluorescent emissions from the dyes. The data collected by both detectors are correlated (e.g., synchronized, and/or super-positioned for analysis) for accurate data peak identification. | 11-26-2015 |
20150355141 | SIZE MARKER AND METHOD FOR CONTROLLING THE RESOLUTION OF AN ELECTROPHEROGRAM - The invention concerns a size marker for electrophoresis, characterized in that it contains several pairs of molecules the sizes of which are selected to allow the generation of a succession of double-peaks in one same electropherogram, said double-peaks being spaced apart two by two by a distance greater than the distance separating the two peaks of a double-peak. The invention also concerns a method for controlling the resolution of an electropherogram produced with a said marker. | 12-10-2015 |
20150369776 | SYSTEMS AND METHODS FOR SINGLE-MOLECULE DETECTION USING NANOPORES - A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any. | 12-24-2015 |
20150377856 | COMPENSATED PATCH-CLAMP AMPLIFIER FOR NANOPORE POLYNUCLEOTIDE SEQUENCING AND OTHER APPLICATIONS - A compensated patch-clamp system for polynucleotide sequencing and other applications. | 12-31-2015 |
20160011169 | NANOPORE SEQUENCING METHODS | 01-14-2016 |
20160018360 | MICROFLUIDIC DEVICE FOR DETECTING NUCLEIC ACIDS AND ASSOCIATED METHODS - Described is a device that is able to distinguish different nucleic acid concentrations in whole blood or plasma sample directly in under 10 min. By using this device, cell-free DNA in whole blood or plasma can be quantified without extensive sample preparation. The device contains a sample channel, an accumulation channel and a pair of electrodes. Optionally, the device includes power supply connected to the electrodes for generating an electric field to move the DNA from the sample channel to the collection channel, and a sensor to quantify the amount of cell free DNA in the collection channel. Also provided are methods for providing a prognosis for a subject with sepsis or suspected of having sepsis comprising quantifying nucleic acid concentrations in a sample from a subject using a device as described herein. | 01-21-2016 |
20160033451 | SINGLE MOLECULE FILTER AND SINGLE MOLECULE ELECTROGRAPH, AND PROCESS FOR MAKING AND USING SAME - A single molecule filter includes: a membrane including: a first surface; a second surface; and a membrane aperture disposed in the membrane and traversing the membrane from the first surface to the second surface, the membrane aperture provided to communicate a single molecule across the membrane; and a nanotube disposed on the membrane and including: a first end disposed on the first surface of the membrane; a second end disposed distal to the first surface; and a tubular aperture extending along the nanotube from the first end to the second end, the tubular aperture provided to communicate the single molecule from the second end of the nanotube to the membrane aperture. | 02-04-2016 |
20160041122 | METHODS AND APPARATUS FOR TRAPPING AND SIZE RESOLUTION OF NANOPARTICLES AND NANOBUBBLES - Provided are systems and methods for accurate size determination of nanoparticles and nanobubbles, comprising detecting multiple repeated translocations of a captured nanoparticle or nanobubble across the sensing zone of a conical nanopore in fluid communication with a fluid comprising nanoparticles or nanobubbles. | 02-11-2016 |
20160054260 | METHOD AND APPARATUS ANALYZING A TARGET MATERIAL - Aspects of the subject disclosure may include, for example, an apparatus including a material having one or more atomic layers with two or less degrees of freedom for motion of charges in the material, and a gate coupled to the material for controlling charge concentration of the material. The material can have constricted sides, a first through-hole, and a first port and a second port for conduction of charges in the material. The gate can have a second through-hole that is at least partially aligned with the first through-hole. A first voltage potential can be applied to the first port and the second port, along with a second voltage potential applied to the gate which adjusts the charge concentration of the material. A sensor can be used to measure a change in electrical properties of the material caused by a target material traversing the first through-hole of the material. Additional embodiments are disclosed. | 02-25-2016 |
20160054261 | USE OF MICROFLUIDIC SYSTEMS IN THE ELECTROCHEMICAL DETECTION OF TARGET ANALYTES - The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes. | 02-25-2016 |
20160077051 | METHOD OF USING TUMOUR RNA INTEGRITY TO MEASURE RESPONSE TO CHEMOTHERAPY IN CANCER PATIENTS - Cancerous tumours vary significantly in their response to chemotherapy agents. Currently, it is difficult to reliably assess the level of tumour responsiveness to a chemotherapy regimen during or post-administration. Biomarkers of tumour sensitivity to chemotherapy agents have hitherto been unknown. Such a biomarker would expedite identification of nonresponsive patients, who may then switch to other, possibly more effective regimens. The present invention provides a method for determining tumour responsiveness to a chemotherapy agent, wherein RNA is isolated from tumour cells of a patient before, during, and after chemotherapy. The quality of the RNA can be determined by capillary electrophoresis and assignment of an RNA integrity number (RIN). RIN values during and/or after chemotherapy are inversely proportionate to the level of tumour responsiveness. The tumour RIN is an easily accessed biomarker of tumour responsiveness to chemotherapy. The tumour RIN may also be used to assess the efficacy of a chemotherapy regimen. | 03-17-2016 |
20160077052 | ANALYSIS AND ASSAY OF GLYCATED HAEMOGLOBINS BY CAPILLARY ELECTROPHORESIS, BUFFER COMPOSITIONS AND KITS FOR CAPILLARY ELECTROPHORESIS - The invention relates to a method for analysis by capillary electrophoresis of glycated haemoglobins comprising at least one globin chain comprising a glucose residue bound to the amino acid in the N-terminal position, contained in a biological sample, said method comprising using a buffer composition comprising at least one compound which is capable of specifically complexing glucose residues of one or several glycated haemoglobin(s) and of providing said glycated haemoglobin(s) with several negative electric charges at an alkaline pH. By way of example, this compound may be 3,4- or 3,5-dicarboxyphenylboronic acid, preferably 3,5-dicarboxyphenylboronic acid. Said method may in particular be used to separate and assay haemoglobin HbA | 03-17-2016 |
20160123923 | EXPORTING MEASUREMENTS OF NANOPORE ARRAYS - A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics. | 05-05-2016 |
20160146766 | ELUATE ANALYSIS AND COLLECTION - The invention relates to analysing and controlling collection of liquid eluate output from a separation process, in particular by use of a measure of suspended material in the eluate based on a light scattering detection method. Exemplary embodiments include a method ( | 05-26-2016 |
20160153960 | ANALYSIS DEVICE | 06-02-2016 |
20160169864 | FIBER COATED NANOPORES | 06-16-2016 |
20160178554 | NANOPORE-BASED SEQUENCING WITH VARYING VOLTAGE STIMULUS | 06-23-2016 |
20190144262 | PARTICLE EXTRACTION APPARATUS AND PARTICLE EXTRACTION METHOD | 05-16-2019 |
20190145950 | NANOPORE SENSOR, STRUCTURE AND DEVICE INCLUDING THE SENSOR, AND METHODS OF FORMING AND USING SAME | 05-16-2019 |