TAKARA BIO INC. Patent applications |
Patent application number | Title | Published |
20160017300 | METHOD FOR USING HEAT-RESISTANT MISMATCH ENDONUCLEASE - Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method. | 01-21-2016 |
20150329872 | EXPRESSION CASSETTE - The present invention provides an expression cassette comprising ( | 11-19-2015 |
20150291980 | CELL CAPABLE OF PRODUCING ADENO-ASSOCIATED VIRUS VECTOR - According to the present invention, an AAV vector having a higher titer compared with those of conventional ones can be produced using a cell into which a nucleic acid capable of expressing miRNA is introduced artificially. An AAV vector produced using the cell and a composition containing the viral vector as an active ingredient are very useful as gene transfer means in the studies or clinical practice of gene therapies. | 10-15-2015 |
20140363849 | METHOD FOR IMPROVING NUCLEIC ACID SYNTHESIS REACTION - Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ω-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ω-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ω-amino acid. | 12-11-2014 |
20140322793 | NOVEL DNA POLYMERASES - Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A | 10-30-2014 |
20140242701 | CHIMERIC ANTIGEN RECEPTOR - Provided are a chimeric antigen receptor comprising an extracellular domain capable of binding to an antigen, a transmembrane domain and at least one intracellular domain, the chimeric antigen receptor being characterized in that an intracellular domain of a glucocorticoid-induced tumor necrosis factor receptor (GITR) is contained as the intracellular domain; a nucleic acid encoding the chimeric antigen receptor; a cell expressing the chimeric antigen receptor; and a method for producing the cell. | 08-28-2014 |
20140212972 | PROCESS FOR PRODUCING CYTOTOXIC LYMPHOCYTE - The present invention relates to a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one of induction, maintenance and expansion of a cytotoxic lymphocyte in the presence of fibronectin, a fragment thereof or a mixture thereof. | 07-31-2014 |
20140170709 | VECTOR FOR GENE THERAPY - The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections. | 06-19-2014 |
20140120124 | CELL CAPABLE OF EXPRESSING EXOGENOUS GITR LIGAND - Disclosed are: a cell capable of expressing an exogenous GITRL or an exogenous GITRL derivative; a method for producing the cell; a therapeutic or prophylactic agent comprising the cell as an active ingredient; use of the cell in the manufacture of a therapeutic or prophylactic agent; a method comprising a step of administering the cell to a subject; a viral vector carrying a gene encoding a GITRL or a GITRL derivative; a therapeutic or prophylactic agent comprising the viral vector as an active ingredient; use of the viral vector in the manufacture of a therapeutic or prophylactic agent; and a method comprising a step of administering the viral vector to a subject. | 05-01-2014 |
20130115199 | T-CELL RECEPTOR AND NUCLEIC ACID ENCODING THE RECEPTOR - A polypeptide comprising a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 5 of Sequence Listing or a polypeptide consisting of an amino acid sequence having deletion, addition, insertion or substitution of one to several amino acid residues in the sequence, the polypeptide being capable of constituting an HLA-A24-restricted, MAGE-A4 | 05-09-2013 |
20130102048 | METHOD OF RETROVIRUS STORAGE - A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon. | 04-25-2013 |
20130065281 | METHOD FOR SYNTHESIZING cDNA - A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields. | 03-14-2013 |
20120270309 | THERMAL CYCLER - A thermal cycler for incubating reaction mixture, the thermal cycler including:(1) a heat block for holding and heating reaction containers which contain the reaction mixture, the heat block being a double-layered structure of a lower layer heat block and an upper layer heat block; and (2) a thermal control means for controlling a temperature of the lower layer heat block and a temperature of the upper layer heat block independently and respectively and keeping the temperature of the upper layer heat block higher than the temperature of the lower layer heat block while incubating the reaction mixture. Since the thermal cycler prevents the occurrence of the condensation of water or other components of the reaction mixture in the reaction container and the thermal differences based on the installation positions of the reaction containers, the PCR method and the other enzyme reaction can be performed in a good repeatability. | 10-25-2012 |
20120164654 | COMPOSITION FOR REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION - A composition for a reverse transcription polymerase chain reaction, which comprises a thermostable DNA polymerase, a reverse transcriptase, a dye marker and a specific gravity-increasing agent; and a premix reagent for a one-step RT-PCR, which comprises the composition, is not frozen under usual storage conditions at −20 to −30° C. and has excellent handleability. | 06-28-2012 |
20120083590 | POLYPEPTIDES HAVING DNA POLYMERASE ACTIVITY - A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide. | 04-05-2012 |
20120021420 | Synthetic siRNA Detection Method - A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3′ end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3′ end is DNA, comprising: (a) adding polydeoxyadenosine to the 3′ DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5′ side to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by a reverse transcription; and (c) detecting the DNA synthesized in (b). | 01-26-2012 |
20110278229 | Centrifugation Method - A centrifugation method for separating a high specific gravity substance and a low specific gravity substance from a mixture thereof, which prevents the contamination of the high specific gravity substance in collecting the low specific gravity substance after a separation operation, is provided. The centrifugation method includes centrifuging a mixture comprising a high specific gravity substance, a low specific gravity substance, and at least one of a thermoreversible gel and a dissolved gelling agent capable of forming a thermoreversible gel, in which the thermoreversible gel or the dissolved gelling agent forms a gel layer which separates the low specific gravity substance and the high specific gravity substance. The centrifugation is preferably carried out at a temperature equal to or lower than the gelation temperature of the gelling agent. | 11-17-2011 |
20110117653 | METHOD FOR PRODUCTION OF PLURIPOTENT STEM CELL - The present invention relates to a method for production of a cell population containing a pluripotent stem cell, said method comprising a step of treating a somatic cell which has been contacted with nuclear reprogramming factors under nutrient-starved condition, and/or a step of treating the somatic cell with an agent capable of arresting cell cycle. The present invention allows induction and growth of pluripotent stem cells at high frequency, and it also allows production of pluripotent stem cells with high efficiency. The nuclear reprogramming factors to be used may be any selected from the group consisting of OCT4, SOX2, c-MYC, KLF4, NANOG and LIN28. | 05-19-2011 |
20110027242 | METHOD FOR PRODUCTION OF TRANSFECTED CELL - Disclosed is a method for producing a cell population into which a desired gene is introduced, which is characterized by comprising the following steps (1) and (2): (1) culturing a cell population containing a T-cell and/or a progenitor cell of a T-cell in a vessel containing fibronectin or a fragment thereof and a CD3 ligand; and (2) adding a vector carrying the desired gene to the vessel of the step (1). By carrying out both the steps (1) and (2) in the same vessel, the gene transfer can be achieved with high efficiency. | 02-03-2011 |
20100317613 | ACTIVITY ENHANCER FOR DETOXIFYING ENZYME - It is intended to provide a drug, a food or a feed which has an effect of enhancing the activity of a second-phase detoxifying enzyme and an effect of increasing intracellular glutathione content. | 12-16-2010 |
20100248262 | Anti-Fibronectin Fragment Monoclonal Antibody - A method for measuring a fibronectin fragment which is easy to handle and has excellent measuring accuracy, specificity and reproducibility is provided. An anti-fibronectin fragment monoclonal antibody which reacts with a human fibronectin fragment but does not react with human fibronectin, a measuring reagent containing the monoclonal antibody, a method for measuring a fibronectin fragment which uses the monoclonal antibody and a hybridoma which produces the monoclonal antibody are provided. | 09-30-2010 |
20100150886 | Method of Producing Lymphocytes - A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand. | 06-17-2010 |
20100143979 | POLYPEPTIDES HAVING DNA POLYMERASE ACTIVITY - A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide. | 06-10-2010 |
20100139157 | FUNGAL BED CULTIVATION METHOD OF HON-SHIMEJI MUSHROOM - A fungal bed cultivation method of a hon-shimeji mushroom, in which the sprouting step and/or fruit body growing step is carried out under an environmental condition of high CO | 06-10-2010 |
20100137415 | VECTOR FOR GENE THERAPY - The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections. | 06-03-2010 |
20100137228 | DRUGS, FOODS OR DRINKS WITH THE USE OF ALGAE-DERIVED PHYSIOLOGICALLY ACTIVE SUBSTANCES -
Medicinal compositions for treating, ameliorating or preventing diseases with sensitivity to 3,6-anhydrogalactopyranose represented by formula (1):
| 06-03-2010 |
20100105867 | PROCESS FOR PRODUCING OSTEOCALCIN-CONTAINING EXTRACT - A process for producing an extract containing activated osteocalcin; an extract which can be obtained by this production process; a food, a drink, a drug, an oral cavity-care composition and a feed containing this extract; and a growth enhancer containing osteocalcin originating in a bone. | 04-29-2010 |
20100068192 | Method for Production of T Cell Population - A method for preparing a T cell population, wherein the T cell population expresses CD45RA and expresses at least one selected from the group consisting of CD62L, CCR7, CD27, and CD28, characterized in that the method comprises the step of culturing a cell population comprising a T cell, in the presence of fibronectin, a fragment thereof or a mixture thereof. | 03-18-2010 |
20100048491 | DRUGS, FOODS OR DRINKS WITH THE USE OF ALGAE-DERIVED PHYSIOLOGICALLY ACTIVE SUBSTANCES -
Medicinal compositions for treating, ameliorating or preventing diseases with sensitivity to 3,6-anhydrogalactopyranose represented by formula (1):
| 02-25-2010 |
20090324566 | T-Cell Receptor and Nucliec Acid Encoding the Receptor - A polypeptide comprising a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 5 of Sequence Listing or a polypeptide consisting of an amino acid sequence having deletion, addition, insertion or substitution of one to several amino acid residues in the sequence, the polypeptide being capable of constituting an HLA-A24-restricted, MAGE-A4 | 12-31-2009 |
20090269809 | THERMOSTABLE RIBONUCLEASE H - A polypeptide having an RNaseH activity and being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide. | 10-29-2009 |
20090221065 | COMPOSITION FOR SUPPRESSING THE EXPRESSION OF FUCOSYLTRANSFERASE - Disclosed is a means for reducing the expression amount of a FUT8 gene, reducing the expression amount of a FUT8 protein, and/or reducing the expression amount of a product produced by the action of FUT8. | 09-03-2009 |
20090162936 | Method For Transfer Of Gene Into Fat Cell Or Progenitor Fat Cell - A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4. | 06-25-2009 |
20090148926 | HON-SHIMEJI MUSHROOM-FUNGAL BED CULTURE - Problem: To provide a fungal bed culture enabling stable production of a hon-shimeji mushroom in large scale commercial cultivation as well as a method of fungal bed cultivation of a hon-shimeji mushroom using the fungal bed culture. | 06-11-2009 |
20090148911 | DNA POLYMERASES WITH ENHANCED LENGTH OF PRIMER EXTENSION - A formulation and kit of thermostable or other DNA polymerases comprising at least one thermostable or other DNA polymerase which lacks 3′-exonuclease activity, and at least one thermostable DNA polymerase exhibiting 3′-exonuclease activity. Also provided is an improved method for enzymatic extension of DNA strands, especially while, but not limited to, amplifying nucleic acid sequences by polymerase chain reaction wherein the above formulation is made and used to catalyze primer extension. | 06-11-2009 |
20090098600 | THERMOTOLERANT RIBONUCLEASE H - Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides. | 04-16-2009 |
20090053383 | ANGELICA KEISKEI TEA - According to the present invention, there are provided kneading-free | 02-26-2009 |
20090047709 | NOVEL ENDORIBONUCLEASE - A novel endoribonuclease activity exhibiting polypeptide; a nucleic acid coding for the polypeptide; a recombinant DNA comprising the nucleic acid; a transformant obtained by transformation using the recombinant DNA; a process for producing the polypeptide, characterized by including the steps of culturing the transformant and collecting the polypeptide from the culture; a process for producing single-strand RNA fragments, characterized by including the step of causing the polypeptide to act on a single-strand RNA; and a method of fragmenting a single-strand RNA. | 02-19-2009 |
20090047705 | MICROORGANISM-DERIVED PSYCHROPHILIC ENDONUCLEASE - A polypeptide having an endonuclease activity derived from a psychrophilic microorganism | 02-19-2009 |
20090029416 | HYPERTHERMOSTABLE PROTEASE GENE - There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene. | 01-29-2009 |
20090025286 | METHOD FOR CULTIVATING HON-SHIMEJI MUSHROOM ON FUNGAL BED - According to the present invention, there is provided a fungal bed cultivation method of a hon-shimeji mushroom, wherein induction of primordia of a fruit body is conducted by illumination under incubation conditions, and an ongoing culture for fungal bed cultivation of a hon-shimeji mushroom, wherein primordia of a fruit body have been formed thereon during incubation. The culture medium is maintained in a clean state and it is advantageous because the formation of primordia of a fruit body can be carried out in a clean environment and the culture medium wherein primordia of a fruit body have been formed can be transferred to distant mushroom cultivation facilities under clean conditions as such. | 01-29-2009 |
20090011485 | METHOD OF RETROVIRUS STORAGE - A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon. | 01-08-2009 |
20080295399 | METHOD FOR FUNGAL BED CULTIVATION OF MUSHROOM - Problem: To provide a method for fungal bed cultivation of a mushroom of a large size having an excellent shape and crunchy texture, a mushroom cutting useful for the fungal bed cultivation method, a culture medium for fungal bed cultivation into which the cutting is transplanted, and a culture medium suitable for fungal bed cultivation. | 12-04-2008 |
20080293141 | Cell for Producing Retrovirus Vector - The N-acetylglucosaminyltransferase III activity is enhanced in a cell carrying retrovirus-origin gag-pol gene and env gene. By constructing a retrovirus vector with the use of the above cell, a retrovirus vector having a modified sugar chain structure can be obtained. The retrovirus vector constructed by this method shows a high infection efficiency particularly in the presence of a functional substance. | 11-27-2008 |
20080268073 | CERAMIDASE INHIBITOR - The present invention provides a ceramidase activity inhibitor which inhibits ceramidase activity, specifically neutral/alkaline ceramidase activity, characterized in that the inhibitor comprises, as an active ingredient, a processed product derived from at least one plant selected from the group consisting of plants belonging to Ginkgoaceae, plants belonging to Cucurbitaceae, plants belonging to Rutaceae, plants belonging to Laminariaceae, plants belonging to Myrtaceae and plants belonging to Compositae, and a medicament, a quasi-drug, cosmetics and a food, each comprising the inhibitor. | 10-30-2008 |
20080227204 | PROCESS FOR PRODUCING CYTOTOXIC LYMPHOCYTE - The present invention relates to a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one of induction, maintenance and expansion of a cytotoxic lymphocyte in the presence of fibronectin, a fragment thereof or a mixture thereof. | 09-18-2008 |
20080206873 | COMPLEX - A complex comprising a double-stranded DNA containing a terminal repeated sequence originating in a retrovirus and a DNA having a base sequence which does not occur in this retrovirus and a retrovirus-origin protein component. | 08-28-2008 |
20080206811 | Process For Producing Polypeptide - A process for producing a target protein at low temperature, comprising inducing expression of not only a vector having introduced therein a gene coding for the target protein but also a vector having a chaperone gene introduced therein. | 08-28-2008 |