Qiagen GMBH Patent applications |
Patent application number | Title | Published |
20160048564 | METHODS AND SYSTEMS FOR INTERPRETATION AND REPORTING OF SEQUENCE-BASED GENETIC TESTS USING POOLED ALLELE STATISTICS - Disclosed herein are system, method, and computer program product embodiments for building a community database of allele counts. An embodiment operates by receiving human variant datasets derived from samples generated by distinct users, wherein the users consented to share pooled variant observations with other users; determining that a plurality of variant observations meet the inclusion criteria for a pool; and calculating one or more anonymized allele statistics from the pool. | 02-18-2016 |
20160030938 | CLOSING ARRANGEMENT AND METHOD OF CLOSING TUBE - Closing arrangement for a cap of a tube in a carrier, wherein the arrangement comprises at least a first, a second and a third moveable engagement member, the first member being adapted to induce a first part of the cap rotation and the second member being adapted to induce a second part of the cap rotation and the third member being adapted to induce a third part of the cap rotation for closure. | 02-04-2016 |
20160001288 | Tube for Chemical, Biological or Biotechnology Matter, Tube Arrangement, Carrier for Use in a Tube Arrangement, Use of a Tube, And Method for Forming a Tube - A tube for chemical, biological or biotechnological matter is provided, wherein the tube has a positioning element, the positioning element shaped for engagement in a corresponding structure provided in a carrier. | 01-07-2016 |
20150379193 | METHODS AND SYSTEMS FOR INTERPRETATION AND REPORTING OF SEQUENCE-BASED GENETIC TESTS - Disclosed herein are system, method, and computer program product embodiments for aiding in the interpretation of variants observed in clinical sequencing data. An embodiment operates by receiving clinical trial enrollment criteria from a user, including but not limited to genetic targeting criteria; searching a knowledge base of patient test information received from a plurality of independent entities for patients that match the clinical trial enrollment criteria; and providing to the user search results for consented patients that match the clinical trial enrollment criteria. | 12-31-2015 |
20150292000 | CONTROL FOR DIAGNOSTIC ASSAY - A method for determining whether a lysate contains sufficient biological sample material for a nucleic acid amplification reaction that includes preparing the lysate in the presence of at least one compound that inhibits the amplification reaction if insufficient biological sample material was present during preparation of the lysate, but does not inhibit the amplification reaction if sufficient biological sample material was present during preparation of the lysate, subjecting the lysate to the amplification reaction, and analyzing a result of the amplification reaction. Due to the presence of the compound in the amplification reaction, no amplification signal is obtained if insufficient biological sample material was present during preparation of the lysate but an amplification signal is obtained if sufficient biological sample material was present during preparation of the lysate. The invention allows for reliable identification of false negatives that occur because insufficient sample material was subjected to the amplification assay. | 10-15-2015 |
20150284794 | Sequencing Performance With Modified Primers - Methods are described which utilize modified sequencing primers that bind to template with high specificity and stability to improve sequencing performance. In one embodiment, the method utilizes sequencing primers having 3′ and 5′ ends, comprising a minor groove binder (MGB) molecule linked to the 5′ end. In one embodiment said primer further comprises a 5′ flap and said MGB molecule is linked to the 5′ flap. | 10-08-2015 |
20150284788 | Sequencing Performance With Additives - Methods are described which utilize additives that ease secondary structure of DNA to improve sequencing performance. In one embodiment, said additive is betaine. The addition of betaine leads to decreased error rates. | 10-08-2015 |
20150275268 | STABILISATION OF BIOLOGICAL SAMPLES - The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample and for stabilizing the transcriptome of contained cells. | 10-01-2015 |
20150232831 | METHOD FOR ISOLATING RNA INCLUDING SMALL RNA WITH HIGH YIELD - The present invention provides a method for isolating RNA including small RNA having a size of 200 nt or less from a sample, comprising the following steps: a) providing a composition comprising RNA and a chaotropic agent; b) adding alcohol; c) incubating the mixture for at least 2 min; d) adding additional alcohol to the mixture to adjust the overall alcohol concentration in the mixture to ≧50%; e) binding RNA contained in the mixture to a nucleic acid binding solid phase; f) optionally washing the bound RNA; g) optionally eluting RNA from the solid phase. Due to the step-wise addition of alcohol, the overall RNA yield and the yield of small RNA is improved. | 08-20-2015 |
20150204843 | METHOD FOR OBTAINING BLOOD PLASMA FROM A WHOLE BLOOD SAMPLE - A method for obtaining blood plasma from a whole blood sample comprising the following steps a) contacting the whole blood sample with a composition (A) comprising at least one carboxylic acid, wherein the addition of the acidic composition (A) and optionally further additives to the whole blood sample provides a sample mixture having a pH that lies in a range from 2.5 to 5; b) binding red and white blood cells to a magnetic solid phase; wherein step a) and step b) can be performed sequentially or simultaneously, c) separating the solid phase with the bound cells from the remaining sample thereby providing blood plasma. | 07-23-2015 |
20140356860 | METHOD FOR ISOLATING NUCLEIC ACIDS FROM A VETERINARY WHOLE BLOOD SAMPLE - The present invention provides a method for isolating nucleic acids from a veterinary whole blood sample, said method comprising at least the following steps a) preparing a binding mixture comprising—the lysed sample—at least one chaotropic agent—at least one alcohol—at least one polyoxyethylene fatty alcohol ether; b) passing the binding mixture through a column comprising a nucleic acid binding solid phase thereby binding the nucleic acids to the nucleic acid binding solid phase; c) optionally washing the nucleic acids while being bound to the solid phase; d) optionally eluting the nucleic acids from the solid phase. It was found that the addition of the specific non-ionic detergent overcomes the problems of the prior art methods, wherein the column clogs what prevents the efficient nucleic acid isolation from this difficult sample. When the specific non-ionic detergent is included into the binding mixture, no clogging occurs thereby allowing the efficient isolation of nucleic acids from veterinary whole blood samples. | 12-04-2014 |
20140248606 | CELL- OR VIRUS SIMULATING MEANS COMPRISING ENCAPSULATED MARKER MOLECULES - The present invention refers to a method, a composition and a kit for isolating biomolecules from any biological sample material containing cells, virus(es), microorganism(s) or a combination thereof comprising a cell- or virus-simulating means, wherein said cell- or virus-simulating means comprises at least one type of marker molecule(s), incorporated in at least one type of a layer, capsule, bead, sphere or particle, which is not a biological cell or provided on a substrate covered by a coating. | 09-04-2014 |
20140234834 | METHOD FOR QUANTIFYING HUMAN DNA - The invention provides for a method for quantifying one or more nucleic acids of a genome in a sample comprising the steps of (a) amplifying a first nucleic acid to be quantified, (b) determining the amount of said first nucleic acid by comparison of the amount of amplification product from said first nucleic acid with at least one amplification product from a second template nucleic acid, (c) wherein said second template nucleic acid was generated using whole genome amplification and wherein the starting concentration of the second template nucleic acid is known. | 08-21-2014 |
20140212884 | COMPOSITION AND METHODS FOR RT-PCR COMPRISING AN ANIONIC POLYMER - The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an RNA template and further nucleic acid replication. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase-polymerase chain reaction. | 07-31-2014 |
20140199689 | METHOD FOR ISOLATING NUCLEIC ACIDS - The present invention pertains to a method for isolating nucleic acids from a sample, preferably a blood sample, comprising the following steps: a) obtaining a sample which has been stabilised by the use of at least one cationic detergent, wherein the cationic detergent has formed complexes with the nucleic acids; b) obtaining the complexes optionally together with other sample components from the stabilised sample, wherein said complexes comprise the nucleic acids to be isolated; c) resuspending the complexes and optionally adding one or more additives before, during and/or after resuspension, thereby obtaining a resuspended sample comprising at least: i) the nucleic acid to be isolated; ii) at least one chaotropic agent; and iii) at least one chelating agent; and d) isolating nucleic acids from the resuspended sample. It was found that adding a chelating agent during resuspension considerably increases the nucleic acid yield as the formation of precipitates which irreversibly adhere to the container wall is considerably reduced. | 07-17-2014 |
20140158619 | FILTERING DEVICES COMPRISING CLAY MINERALS - The present invention relates to a filtering device comprising clay minerals for the filtration of aqueous solutions used in biochemical and molecular biological applications. In particular, the present invention relates to the removal of undesired proteins from aqueous solutions by filtration through a filter comprising clay minerals. | 06-12-2014 |
20140154748 | THERMOSTABLE CHIMERIC NUCLEIC ACID POLYMERASES AND USES THEREOF - Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as DNA polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized. It is demonstrated that chimeric nucleic acid polymerases of the present invention possess the chemical and physical properties of their component domains (e.g., exonuclease activity, thermostability) and that the chimeric polymerases are thermostable. | 06-05-2014 |
20140154693 | REAGENT USABLE FOR THE ISOLATION AND/OR PURIFICATION OF NUCLEIC ACIDS - The present invention provides a special reagent for the pretreatment of sample materials. The pretreatment with the reagent according to the invention enables the isolation of nucleic acids by a uniform method from very diverse sample materials, in particular different bioprocess samples, even if the nucleic acids are only present in small amounts in the sample materials. The reagent used for the pretreatment comprises, as key component, at least one compound comprising an amino group. The invention further relates to methods of purification of nucleic acids, in which the sample material is pretreated correspondingly, and suitable kits. | 06-05-2014 |
20140147843 | METHOD FOR QUANTIFYING HUMAN DNA USING AN INTERNAL CONTROL - The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined. | 05-29-2014 |
20140140804 | MAGNETIC RACK SYSTEM, METHOD FOR USING A MAGNETIC RACK SYSTEM AND USE OF A MAGNETIC RACK SYSTEM - Magnetic rack system comprising a holder having trough-holes for receiving tubes, a base having a plurality of receptacles with at least one magnet, wherein trough-holes in the holder are arranged relative to the base such that each tube can be positioned in a respective receptacle in a pre-determined position relative to the magnet, and an adapter, wherein the adapter is designed to allow transmission of motion to the tubes ( | 05-22-2014 |
20140134718 | FILTER MODULE IN BIOMOLECULE ISOLATION - The present invention relates to a device for rapid isolation of target molecules from cell lysates and other liquid mixtures comprising particulate material, a method for isolating the target molecules, in particular nucleic acids, using said device and a kit for carrying out said method comprising said device. | 05-15-2014 |
20140113302 | METHODS FOR SEQUENCING, AMPLIFICATION AND DETECTION OF NUCLEIC ACIDS COMPRISING INTERNALLY LABELLED PRIMER - The present application provides for an enhanced method for sequencing, or amplifying and detecting nucleic acids comprising the steps of: i) providing at least one nucleic acid primer comprising at least one labelled nucleotide, ii) providing enzymes and reagents for amplification of a target nucleic acid using said at least one labelled nucleic acid primer, iii) incubating the components of the reaction under conditions suited for amplification of the target nucleic acid primer, iv) detecting the amplified nucleic acids via the labelled nucleotide, wherein the nucleic acid primer comprises the following sequence: 5′-N | 04-24-2014 |
20140087956 | NOVEL COMBINATION OF FLUORESCENT DYES FOR THE DETECTION OF NUCLEIC ACIDS - The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY 632 and DY-520XL. | 03-27-2014 |
20140057263 | MODIFIED HYBRIDIZATION PROBES - The invention relates to a method for detecting a nucleic acid of an organism in a composition, comprising the steps of, (i) amplifying the nucleic acid to be detected, (ii) during or after amplification, hybridizing to said nucleic acid to be detected a first probe that comprises an abasic site additionally optionally carrying a detectable label, (iii) wherein the position of the abasic site corresponds to a position in said nucleic acid to be detected, known to have a polymorphism in said organism, and wherein said nucleic acid is detected if hybridization occurs. | 02-27-2014 |
20130314697 | FLUID PROCESSING TUBE FOR OPTICAL ANALYSIS AND METHOD FOR ANALYZING A FLUID - Fluid processing tube for use in optical analysis comprising at least one first portion being made from a first material suitable for optical analysis and being configured to include two optical paths of different lengths, and at least one second portion connected to said first portion and being made from a second material different from said first material. | 11-28-2013 |
20130280787 | EXTRACTION OF NUCLEIC ACIDS FROM WAX-EMBEDDED SAMPLES - The present invention relates to a method for extracting nucleic acids from a wax-embedded sample, the use of particular solvents for removing wax from a wax-embedded sample in a method for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample as well as to a kit for extracting, isolating and/or purifying nucleic acids from a wax-embedded sample. | 10-24-2013 |
20130280728 | METHOD AND KIT FOR PROCESSING WAX-EMBEDDED BIOLOGICAL SAMPLES - The present invention relates to a method for processing a wax-embedded biological sample, the use of poly(organosiloxane)s for liquefying the embedding medium of a wax-embedded biological sample and a kit for processing a wax-embedded biological sample. | 10-24-2013 |
20130237699 | METHOD AND DEVICE FOR ISOLATING AND PURIFYING DOUBLE-STRANDED NUCLEIC ACIDS - The invention relates to a chromatographic device for isolating and/or purifying double-stranded nucleic acids, preferably double-stranded DNA, from a mixture of such nucleic acids with single-stranded nucleic acids, oligonucleotides, mononucleotides, salts and/or other such impurities. The invention also relates to a method for chromatographically isolating and/or purifying same, and to a kit for this purpose. | 09-12-2013 |
20130236900 | REAGENT FOR THE DISRUPTION OF CELL MATERIAL HAVING A COMPLETELY INTEGRATED INTERNAL STANDARD - The present invention relates to a reagent for the disruption of cell material, containing an internal standard that is completely integrated into the reagent for control and evaluation of the completeness of disruption of the cell material and subsequent steps, comprising a step selected from sample preparation, extraction, enrichment, isolation, purification, reverse transcription, amplification and detection of the cell components obtained from the disrupted cells, or a combination of a plurality or all of these steps. | 09-12-2013 |
20130231460 | METHOD AND DEVICE FOR CONCENTRATING TARGET COMPOUNDS - The present invention relates to a method for concentrating one or more target compounds in a liquid sample, a device for carrying out this method and a kit for processing a biological sample comprising such a device. | 09-05-2013 |
20130224799 | METHOD FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS - A method is disclosed for improved isothermal amplification of nucleic acids comprising the step of release of an essential component from a matrix under predetermined conditions. Furthermore, the invention relates to a kit comprising mesophilic enzyme and a matrix with embedded essential components for isothermal amplification. A composition comprising a matrix and a mesophilic enzyme and a method for embedding a mesophilic enzyme are disclosed as well. | 08-29-2013 |
20130224742 | METHOD FOR DETECTING AND/OR QUANTIFYING HUMAN DNA - The present invention relates to a method, kit and use of various nucleic acid sequences for deleting and/or quantifying one or more nucleic acids of a genome in a sample. Wherein the nucleic acid is amplified and the locus that is amplified is a multi copy locus within the genome, the multicopy locus has copies on at least two different chromosomes and the amplification product is detected and/or quantified. | 08-29-2013 |
20130217092 | Enhancing Reactivation of Thermostable Reversibly Inactivated Enzymes - Disclosed are methods for the enhancement of the reactivation of thermostable reversibly inactivated enzymes comprising reactivating at least one thermostable reversibly inactivated enzyme in the presence of one or more nitrogen containing compounds. | 08-22-2013 |
20130210019 | HELICASE DEPENDENT ISOTHERMAL AMPLIFICATION USING NICKING ENZYMES - The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase. | 08-15-2013 |
20130189726 | METHOD FOR PREPARING AND/OR PROCESSING A BIOLOGICAL SAMPLE USING A MALODOUR COUNTERACTANT - The present invention pertains to a biotechnological method for preparing and/or processing a biological sample, in particular for isolating at least one target biomolecule therefrom, which characterised in that at least one malodour counteractant is used for preventing, reducing, masking and/or suppressing malodour and/or malodour formation during the preparation and/or processing of said biological sample. | 07-25-2013 |
20130164825 | METHOD FOR ISOLATING A TARGET NUCLEIC ACID INCLUDING SMALL TARGET NUCLEIC ACIDS WITH HIGH YIELD - The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids. | 06-27-2013 |
20130158247 | METHOD FOR ISOLATING AND/OR PURIFYING NUCLEIC ACID(S) - The present invention relates to a method for isolating and/or purifying one or more nucleic acid(s) from a sample, comprising the steps of essentially separating the nucleic acid(s) from the sample by binding the nucleic acid(s) to a solid phase by means of a non-chaotropic water-soluble binding ligand at a first pH (pH I), and essentially eluting the nucleic acid(s) from the solid phase at a second pH (pH II). The invention further relates to a kit for isolating and/or purifying nucleic acid(s) from a sample and/or for protecting nucleic acid(s). | 06-20-2013 |
20130144047 | FORENSIC IDENTIFICATION - The invention provides allelic ladder mixtures and individual alleles suitable for use in such mixtures. The allelic ladder mixtures give improved identification and distinguishing capabilities, particularly suitable in forensic investigations. | 06-06-2013 |
20130137586 | STABILIZATION OF NUCLEIC ACIDS IN CELL MATERIAL-CONTAINING BIOLOGICAL SAMPLES - The present invention relates to the use of an aqueous system for stabilizing cell material-containing biological samples while preserving the cell morphology of the cell material and to a method for stabilizing nucleic acids in cell material-containing biological samples while preserving the cell morphology of the cell material. | 05-30-2013 |
20130112622 | NEW LIQUID PROCESSING DEVICE - The present invention refers to a device, comprising a hollow body having at least one open end and at least one barrier inside of the hollow body, which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force like pressure, drag force or driving power to said barrier, wherein said barrier is spaced from at least one open end of the hollow body, the use of such a device for processing of a liquid sample, a method for preparation of said device and a method for isolation or purification of any biomolecules using said device. | 05-09-2013 |
20130109095 | METHOD FOR TRANSFECTING A EUKARYOTIC CELL | 05-02-2013 |
20130105393 | NEW STORAGE, COLLECTION OR ISOLATION DEVICE | 05-02-2013 |
20130095473 | METHOD FOR DETERMINATION OF TARGET CELLS OR TISSUE FOR EXTRACTION OF BIOMOLECULES FROM FIXED BIOLOGICAL SAMPLES - The present invention relates to a method for determination of target cells or tissue for isolating or extracting biomolecules from fixed biological samples, the preparation of a sample in a method for extracting, isolating and/or purifying biomolecules from a fixed biological sample as well as to a kit for visualizing target cells or tissue in a fixed biological sample for extracting, isolating and/or purifying biomolecules from said target cells or tissue. | 04-18-2013 |
20130065776 | SELECTIVE ENRICHMENT OF NON-METHYLATED NUCLEIC ACIDS - The present invention relates to a method for selectively amplifying non-methylated sequences of a DNA comprising the steps of (i) providing a sample comprising a DNA which is methylated at least one site, (ii) treating the DNA in the sample with a methylation-dependent nuclease, and (iii) amplifying the DNA cut using the methylation-dependent nuclease. In addition, the invention relates to kits for use in the method according to the invention. The method according to the invention can be used for selectively preparing to (selectively accumulating) non-methylated sequence segments of genomic DNA and for analysing the global methylation pattern in genomic DNA. | 03-14-2013 |
20130052721 | METHOD FOR ISOLATING RNA FROM A RNA AND DNA CONTAINING SAMPLE - The present invention pertains to a method for isolating RNA, including small RNA from a RNA and DNA containing sample, wherein the sample is lysed and the optionally further processed lysate is incubated with a DNase to degrade DNA prior to purifying the RNA from the optionally further processed lysate. It was found that performing the DNase digest prior to isolating the RNA from the lysate has considerable advantages. | 02-28-2013 |
20130030165 | CHROMATOGRAPHIC DEVICE AND METHOD FOR ISOLATING AND PURIFYING NUCLEIC ACIDS - The present invention relates to a chromatographic device for isolating and purifying nucleic acids, preferably genomic DNA, by gel filtration chromatography, a method for isolating and purifying nucleic acids, preferably genomic DNA, using this device and a kit comprising this device. | 01-31-2013 |
20130030163 | METHOD FOR ISOLATING AND PURIFYING NUCLEIC ACIDS - The present invention relates to a method for isolating and purifying nucleic acids, preferably comprising genomic DNA, from biological samples comprising the steps of lysing the sample using a lysis buffer comprising a source of anionic surfactant ions, optionally disintegrating the RNA present in the lysate, precipitating the surfactant ions from the lysate, and separating the nucleic acids from the precipitate and further contaminants by size-exclusion chromatography. The invention furthermore relates to a lysis buffer, a method of lysing cells and a kit for the isolation and purification of nucleic acids. | 01-31-2013 |
20130026268 | PULVERIZER AND CORRESPONDING METHOD FOR PREPARING A BIOLOGICAL SAMPLE FOR PROCESSING - The invention relates, for example, to methods for preparing a plant or animal sample for processing, i.e. for example for the isolation of nucleic acids or proteins from the sample, as well as to a pulverizer. | 01-31-2013 |
20130023656 | METHOD FOR SELECTIVE ISOLATION AND PURIFICATION OF NUCLEIC ACIDS - The present invention relates to a gentle method for isolating and purifying nucleic acids from a biological cell comprising sample comprising at least DNA, RNA, and proteins, comprising at least the steps of 1. mixing the sample with a lysis buffer, 2. incubating the sample at a temperature within a range between 45° C. and 59° C. to obtain DNA as well as RNA or within a range between 60° C. and 70° C., to obtain DNA essentially free of RNA and 3. separating the nucleic acids from any contaminants. | 01-24-2013 |
20130023655 | METHOD FOR PRECIPITATING ANIONIC SURFACTANT IONS IN THE PRESENCE OF NUCLEIC ACIDS - The present invention relates to a method for selectively precipitating anionic surfactant ions from a liquid sample comprising anionic surfactant ions and nucleic acids, as well as to a kit for the isolation and purification of nucleic acids. | 01-24-2013 |
20130022984 | METHOD FOR THE NON-SPECIFIC ENRICHMENT OF MICROORGANISMS - The present invention relates to a method for the non-specific enrichment of microorganisms from complex starting materials, wherein the starting materials containing the microorganisms are brought in contact with cells of the innate immune system, the microorganisms are bound to the cells of the innate immune system and the binding complex is separated from the complex starting material. | 01-24-2013 |
20120295328 | METHOD FOR ISOLATING SMALL RNA - A method for isolating small RNA from a sample is provided, the method comprising binding the RNA to silica particles by contacting the sample with a) at least one alcohol, b) at least one chaotropic salt comprising a chaotropic anion selected from the group consisting of trichloroacetate, perchlorate and trifluoroacetate and c) silica particles and separating the bound RNA from the rest of the sample. The present invention also provides compositions and kits to efficiently isolate small RNA molecules from samples, in particular biological samples such as blood, blood products tissue and body fluids. | 11-22-2012 |
20120283426 | Methods and Kit for Isolating Nucleic Acids - The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention. | 11-08-2012 |
20120231446 | METHOD FOR SELECTIVELY ENRICHING AND ISOLATING MICROBIAL AND OPTIONALLY ADDITIONAL VIRAL NUCLEIC ACIDS - The present invention relates to a method for selectively enriching and/or isolating microbial and optionally additionally viral nucleic acids from samples that contain a mixture of microbial cells, freely circulating nucleic acids and higher eukaryotic cells, and optionally additionally viruses, in a liquid, and to a kit for selectively enriching and/or isolating intracellular and extracellular microbial nucleic acids, and optionally additionally viral nucleic acids, from samples that contain a mixture of microbial and higher eukaryotic cells, freely circulating nucleic acids, in particular extracellular microbial nucleic acids, and optionally additionally viruses in a liquid. | 09-13-2012 |
20120219953 | METHOD FOR DETECTING AND QUANTIFYING POLY(A) RNA AND MRNA - The present invention relates to a method for detecting and/or for quantifying poly(A) RNA and/or mRNA, wherein a poly(dT) oligonucleotide which features a fluorescent dye and also a quencher is hybridized to the nucleic acid to be detected. Non-hybridized poly(dT) oligonucleotide is degraded by an added nuclease, and as a result, fluorescently labelled nucleotides are released and the resulting fluorescent signal is measured. | 08-30-2012 |
20120171728 | PROCESS FOR AMPLIFYING DNA USING TETRATETHYLENE GLYCOL, KIT OF PARTS THEREFOR AND USE THEREOF - Disclosed is a process for amplifying DNA, a process for preparing and amplifying DNA, a kit of parts for DNA amplification and DNA preparation, and the use thereof, all of which are characterized by the use of tetraethylene glycol. Moreover, the use of tetraethylene glycol in a reaction solution by carrying out a DNA amplification and a reaction solution containing tetraethylene glycol for carrying out a DNA amplification are disclosed. | 07-05-2012 |
20120171675 | METHOD FOR ISOLATING NUCLEIC ACIDS - The invention describes a method of and kits for isolating and/or purifying nucleic acids, more specifically short-chain nucleic acids such as miRNA, from a nucleic acid-containing starting material, characterized by the following method steps of:
| 07-05-2012 |
20120160355 | VALVE - The invention relates to a system comprising a rotary valve and an actuating device for the rotary valve. The invention further relates to a related flow system having a rotary valve. The flow system is in particular a micro-flow system. The system comprises at least two separate components, wherein the one component is a rotary valve comprising a hollow cylindrical mount ( | 06-28-2012 |
20120149011 | METHOD FOR DETECTING TARGET NUCLEIC ACIDS - The invention relates to a method for detecting target nucleic acids which are detected by means of a specific sequence tag which is not part of the target nucleic acid. | 06-14-2012 |
20120130061 | Method FMethod For Isolating And Purifying Nucleic Acids - The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples and biological materials. The present invention further relates to a kit for carrying out the method of the invention. | 05-24-2012 |
20120107818 | DETECTION OF MULTIPLE NUCLEIC ACID SEQUENCES IN A REACTION CARTRIDGE - The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising the following steps, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set, wherein (a) each probe set consists of at least three probes, (b) each of the probes is specific for a nucleic acid sequence, (c) there are at least two probes in each set which carry an identical label, (d) each of the probes in a given probe set that carries an identical label has a melting temperature (T | 05-03-2012 |
20120077969 | NUCLEIC ACID PURIFICATION METHOD - The present invention relates to a method for purifying a defined amount of nucleic acids from a nucleic acid-containing sample, which has at least the following steps: (a.) contacting the nucleic acid-containing sample with a defined amount of a nucleic acid binding phase with the following features: (i) the nucleic acid binding phase has nucleic acid binding ligands that have at least one protonatable group; (ii) the nucleic acid binding ligands are bound to a carrier; (iii) the nucleic acid binding phase has a surface with a low charge density, wherein the amount of nucleic acids in the sample exceeds the binding capacity of the amount of nucleic acid binding phase used; (b.) binding of the nucleic acids to the nucleic acid binding phase at a pH (binding pH) that is below the pKs value of at least one of the protonatable groups; (c.) elution of the nucleic acids at a pH that is above the binding pH, wherein a defined amount of nucleic acids is obtained. Furthermore, corresponding kits and nucleic acid binding phases, which can be used for the purification of nucleic acids, are disclosed. | 03-29-2012 |
20120070823 | METHOD AND DEVICE FOR AUTOMATICALLY PROCESSING A SAMPLE - The invention relates to a method and a device for processing a biological sample. According to the invention, the processing comprises binding, washing and eluting biomolecules of the biological sample. The aim of the invention is to provide a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further process by a microfluidic system. According to the invention, a container ( | 03-22-2012 |
20120024417 | Bellows-Type Reservoir For Microsystems - The invention relates to a storage and dispensing vessel for microsystems and to a method for storing and dispensing a liquid. It is the object of the invention to be able to store a liquid with volatile and/or creeping substances in very small vessels over many months, preferably over at least one year, and to be able to dispense it automatically with comparatively little technical effort. For accomplishing this object, a bellows configured as a vessel is provided which is sealed, preferably welded, in an air-tight and liquid-tight manner once the bellows vessel has been filled. This bellows vessel at the same time serves as a dispensing vessel. The vessel can be emptied automatically with relatively simple means. For this purpose, it is, for example, clamped into a device which compresses the bellows, namely in the direction of a hollow needle—also referred to as cannula or hypodermic needle—or of a comparable means. In the process, the hollow needle or the comparable means pierces a vessel wall. The vessel is then automatically emptied via the hollow needle or the comparable means. | 02-02-2012 |
20110311985 | RNAI BASED SELECTION SYSTEM - The present invention provides a novel RNAi based selection system for selecting host cells that have incorporated an expression vector. | 12-22-2011 |
20110281259 | Method And Apparatus For the Simultaneous, Automated Decomposition Of A Plurality Of Biological Samples - The invention relates to a method for decomposing a biological sample ( | 11-17-2011 |
20110244468 | PARALLEL EXTRACTION OF DIFFERENT BIOMOLECULES FROM FORMALIN-FIXED TISSUE - The present invention relates to a method of isolating/extracting in parallel various biomolecules, in particular nucleic acids and proteins, from the same fixed biological samples, to the quantification and analysis of the biomolecules isolated by the method of the invention, and to a kit for isolating/extracting in parallel various biomolecules from a fixed sample, to the use of said kit for diagnosing, prognosing, deciding the therapy of and monitoring the therapy of a disease. | 10-06-2011 |
20110224419 | Method For Isolating And Purifying Nucleic Acids - The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples, and biological materials. The present invention further relates to a kit for carrying out the method of the invention. | 09-15-2011 |
20110224063 | Rotor For A Centrifuge - An apparatus ( | 09-15-2011 |
20110205835 | METHOD FOR SUSPENDING OR RE-SUSPENDING PARTICLES IN A SOLUTION AND APPARATUS ADAPTED THERETO - A method for suspending or re-suspending magnetically attractable particles is provided. In the present method at least a mixing vessel ( | 08-25-2011 |
20110189654 | DIAGNOSTIC REAGENT, CONTAINING BIOPARTICLES, METHOD FOR PRODUCTION THEREOF AND USE THEREOF AS INTERNAL STANDARD IN NUCLEIC ACID PREPARATION AND NUCLEIC ACID DETECTION METHODS - A diagnostic reagent in the form of a composition dimensionally stable under standard conditions, comprising bioparticles and also customary pharmaceutical excipients, wherein the bioparticles are selected from the group consisting of bacteria, viruses, fungi, protozoa, bacteriophages, yeasts, spores, parasites, plant cells, animal or human cells, gametes, plasmids, and viroids. | 08-04-2011 |
20110163255 | METHOD FOR PROTEIN PURIFICATION UNDER DENATURING CONDITIONS - The invention relates to a method for the preparation of an application buffer for the purification of proteins by means of immobilized metal ion affinity chromatography (IMAC) under denaturing conditions, which is characterized in that a defined amount of a buffer concentrate having a defined pH value is mixed with a defined amount of a urea concentrate, whereby an application buffer having a defined pH value is provided. According to the invention, a corresponding kit is provided in addition. The components are stable in storage and by mixing produce an application buffer having a defined composition and a defined pH value. The need for pH adjustment or a new preparation is eliminated. The invention can thus be used in an automated manner and as a closed kit concept. | 07-07-2011 |
20110162755 | APPARATUS AND PROCESS FOR REMOVING SUBSTANCES FROM PRE-FILLED CONTAINERS | 07-07-2011 |
20110160446 | METHOD FOR ISOLATING SHORT-CHAIN NUCLEIC ACIDS - The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterised by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧25% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood. | 06-30-2011 |
20110151459 | SIMULTANEOUS DETECTION OF MULTIPLE NUCLEIC ACID SEQUENCES IN A REACTION - The present invention relates to a method for simultaneously amplifying and detecting nucleic acid sequences in a reaction comprising the following steps: (i) providing a sample comprising at least one nucleic acid molecule; (ii) providing reagents for performing an amplification reaction, wherein the reagents comprise at least four, preferably at least five, more preferably at least six probes, wherein (a) each of the probes is specific for a nucleic acid sequence; (b) at least two, preferably at least three probes carry the same label; and (c) each of the probes that carry the same label has a melting temperature (Tm) which differs by more than 2° C. from the other probes with the same label when they are dissociated from their target nucleic acid sequence by heating; (iii) amplifying the nucleic acid sequences in the reaction; (iv) detecting the amplified nucleic acids by determining whether the labeled probe has bound its nucleic acid sequence; and (v) detecting the temperature at which each given labeled probe dissociates from the nucleic acid sequence to which it has bound. The invention also relates to kits for the use in such a method. | 06-23-2011 |
20110130558 | METHOD FOR ISOLATING NUCLEIC ACIDS - The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterised by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧15% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood | 06-02-2011 |
20110091364 | APPARATUS FOR DELIVERING PIPETTABLE SUBSTANCES | 04-21-2011 |
20110076751 | LYSIS, BINDING AND/OR WASH REAGENT FOR ISOLATING AND/OR PURIFYING NUCLEIC ACIDS - The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids. | 03-31-2011 |
20110071031 | COMPOSITIONS, METHODS, AND KITS FOR ISOLATING AND ANALYZING NUCLEIC ACIDS USING AN ANION EXCHANGE MATERIAL - The present disclosure relates to methods for isolating, amplifying, and/or analyzing nucleic acids in the presence of an anion exchange material by performing the isolation, amplification and/or analysis step in the presence of at least one anionic compound. | 03-24-2011 |
20110054054 | HYDRAZINE-MODIFIED MATRICES AND USE THEREOF IN METHODS FOR ISOLATING BIOMOLECULES - The invention relates to novel functionalized matrices or matrix materials comprising structures of general formula Carrier-Linker-Spacer-NR | 03-03-2011 |
20110039709 | APPARATUS FOR PROCESSING BIOLOGICAL MATERIAL - The invention relates to an apparatus for purification, respectively processing and/or analysis of biological target molecules with a detection device for detecting at least one object, which includes at least one detection area, wherein the detection device is adapted to detect at least one height value of the detection area and is adapted to determine, from the at least one height value, a spatial position and/or orientation and/or a type and/or a presence and/or a number and/or a state of the at least one object. Further, the invention relates to a receiving device for receiving material for the processing, purification and/or analysis of biological target molecules with at least one identification element, wherein the at least one identification element defines a height profile for identifying the receiving device, wherein the height profile is provided for at least one height measurement and extends at least in sections along a line, preferably a straight line. Finally, the invention relates to a method for inventorying, position determining and/or orientation determining of objects, which are needed for the automatic processing of a material, which contains biological target molecules, on a corresponding apparatus, in which at least one height value of a detection area of the respective object is detected, and, from the height value, a spatial position and/or an orientation and/or a type and/or a presence and/or a number and/or a state of the respective object is determined on the apparatus. | 02-17-2011 |
20110034535 | POSITIVE CONTROLS FOR EXPRESSION MODULATING EXPERIMENTS - The invention pertains to the use of an apoptosis inducing combination of at least a. a first expression modulating compound silencing the expression of at least a first target gene involved in apoptosis and b. a second expression modulating compound silencing the expression of at least a second target gene involved in apoptosis as a positive control in expression modulating assays. Also provided are suitable methods, kits and compositions. | 02-10-2011 |
20110008846 | MODIFIED ENZYMES AND THEIR USES - The invention relates to an active enzyme of bacterial, fungal, viral, or archae origin, wherein the enzyme is coupled, preferably covalently coupled with at least one polymer having a molecular weight between about 500 to about 20,000 daltons selected from the group consisting of polyethylene glycol and polypropylene glycol. The invention relates in particular to uses thereof of such as in molecular biology as well as kits comprising such enzymes. In preferred embodiments the enzymes of the invention are nucleic acid modifying or replicating enzymes. | 01-13-2011 |
20110008771 | METHOD AND MATERIALS FOR TRIGGERED RELEASE OF A BIOLOGICAL SAMPLE - The invention relates to a method for releasing a biological sample from a solid matrix substantially without disintegration of biomolecules comtained in said biological sample by at least partially transferring the solid state of said matrix into a dissolved or liquid state by changing at least one physico-chemical property of the environment of said matrix. | 01-13-2011 |
20100323363 | METHOD FOR THE TREATMENT OF A SAMPLE CONTAINING BIOMOLECULES - The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R | 12-23-2010 |
20100297614 | PROCESS FOR THE DETECTION OF CYTOSINE METHYLATIONS - The invention concerns a method for the detection of cytosine methylations in DNA and a kit for undertaking an assay according to the method of the invention. | 11-25-2010 |
20100291658 | PROCESS FOR SEPARATING NONPROTEINANEOUS BIOMOLECULES, IN PARTICULAR NUCLEIC ACIDS, FROM PROTEINANEOUS SAMPLES - The present invention relates to method of isolating non-protein-containing biomolecules, in particular nucleic acids, characterized in that protein degradation is carried out on a solid phase. | 11-18-2010 |
20100273984 | METHOD FOR ENRICHING PHOSPHOPEPTIDES - The invention relates to a method for enriching phosphopeptides. Said method is characterized in that a carrier is used which carries phosphate groups and/or phosphonate groups on the surface thereof. The phosphate groups and/or phosphonate groups are functionalized with zirconium ions and are bonded to the carrier by means of linker structures which have at least one alkyl chain containing at least 5 C atoms. Also disclosed are corresponding carriers and suitable kits for enriching phosphopeptides. | 10-28-2010 |
20100267081 | METHOD AND DEVICE FOR FIXING/STABILISING A SAMPLE - The present invention provides a method for fixing and/or stabilizing a sample, in which the sample is put into a permeable container with a maximum overall height of 10 mm, preferably of 5 mm, and the container filled with the sample is immersed in fixing and/or stabilizing agents and the sample is fixed and/or stabilized. | 10-21-2010 |
20100255524 | METHOD FOR STABILISING A BIOLOGICAL SAMPLE - The present invention relates to a method of stabilizing a biological sample, having the steps of preparation of a biological sample, and of contacting the biological sample with a composition, having a substance according to the following structural formula: | 10-07-2010 |
20100209993 | PERMANENT INACTIVATION OF NUCLEASES - The present invention relates to the use of reagent for permanently inactivating nucleases wherein the reagent comprises an oxidizing agent, a protein denaturant and optionally a pH adjustor and to a method for permanently inactivating nucleases using said reagent. | 08-19-2010 |
20100209912 | METHOD FOR THE TREATMENT OF A SAMPLE CONTAINING BIOMOLECULES - The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymers, which comprise these nitrogenous compounds, d) amines of the type R | 08-19-2010 |
20100173295 | FIXATION OF A BIOLOGICAL MATERIAL - The present invention relates to a method for the treatment of a biological material, comprising the steps i) providing a biological material, and ii) contacting the biological material with a first non-aqueous composition comprising: (a1) 10 to 90 vol. % methanol, and (a2) at least one additional additive, and (a3) optionally an acid. iii) transferring the biological material into a second composition (B) comprising up to 99 vol. % ethanol as well as to a new composition for preservation of a biological material usable in said method and the biological material resulting from this method, a method for the analysis of a treated biological material, various kits as well as the use of the composition in such a method. | 07-08-2010 |
20100159532 | METHOD AND DEVICE FOR PERFORMING A NUCLEIC ACID PREPARATION AND/OR AMPLIFICATION - The invention relates to a method and a device for performing a nucleic acid preparation and/or amplification. | 06-24-2010 |
20100137567 | PULVERIZER AND CORRESPONDING METHOD FOR PREPARING A BIOLOGICAL SAMPLE FOR PROCESSING - The invention relates, for example, to methods for preparing a plant or animal sample for processing, i.e. for example for the isolation of nucleic acids or proteins from the sample, as well as to a pulverizer. | 06-03-2010 |
20100120044 | MODIFIED POLYNUCLEOTIDES COMPRISING RIBOSE RINGS - The invention pertains to different methods employing the use of a polynucleotide comprising ribose rings which carry a modification at the 2′-OH group. | 05-13-2010 |
20100113758 | METHOD FOR PURIFYING BIOMOLECULES - The present invention relates to a process for the purification of biomolecules, in particular of nucleic acids, such as DNA and RNA molecules. | 05-06-2010 |
20100099191 | RNA INTERFERENCE TAGS - The invention relates to a composition for inhibiting the expression of a target gene in a eukaryotic cell by RNA interference, the composition comprising a genetic construct and the target gene being introduced into the eukaryotic cell by means of said construct, and the construct having a first target gene and a first siRNA tag. The invention also relates to a composition for inhibiting the expression of one or more target genes in a eukaryotic cell by RNA interference. The composition comprises at least two genetic constructs and the target genes are introduced into the eukaryotic cell by means of said constructs, a) the first construct containing a first target gene and a first siRNA tag and b) the second construct containing a second target gene and a second siRNA tag, and the first and the second siRNA tag having different nucleic acid sequences. The invention finally relates to a method for inhibiting the expression of a target gene in a eukaryotic cell. Said method comprises the following steps: a) providing at least one eukaryotic cell, said cell being capable of RNA interference, b) transfecting the cells with a composition according to the invention and c) introducing at least one siRNA that is complementary to a siRNA tag of a transfected construct to inhibit the expression of the target gene that forms a transcription unit together with the siRNA tag. | 04-22-2010 |
20100075422 | TRANSFECTION MICROARRAYS - A transfection method of introducing sample molecules into cells is provided, comprising:
| 03-25-2010 |
20100056769 | METHODS AND KIT FOR ISOLATING NUCLEIC ACIDS - The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention. | 03-04-2010 |
20100048867 | DEVICE AND PROCESS FOR PURIFYING NUCLEIC ACIDS - An apparatus for purifying nucleic acids by negative chromatography is disclosed, which comprises a hollow body with an opening, respectively, at the top and at the bottom end, said hollow body containing a stationary solid phase, characterized in that the stationary phase contains at least 2 different chromatography resins, such as size e.g. exclusion gel filtration materials (SEC materials), as well as a method for purifying nucleic acids and the use of the apparatus. | 02-25-2010 |
20100036109 | METHOD FOR SELECTIVELY AND REVERSIBLY ADSORBING NUCLEIC ACIDS ON A CARRIER MATERIAL - The invention relates to a process for selective, reversible adsorption of nucleic acids to a support material, which process is characterized in that (a) a nucleic acid-containing mixture having a cosmotropic salt content of at least 1 mole is contacted with said support material, whereupon the nucleic acid(s) is (are) adsorbed from said nucleic acid-containing mixture to said support material, and (b) the nucleic acid(s) bound to the support material is (are) detached from the support material by means of an aqueous solution having a salt content of no more than 1 mole. | 02-11-2010 |
20100021351 | COLLECTION OF BIOLOGICAL SAMPLES - The present invention relates to a container for receiving a liquid biological sample, with a volume for receiving the liquid biological sample, and with a filling and/or removal opening for filling the volume with the sample and for removing the sample from the volume. The invention is distinguished in that a constriction or partition forming an aperture is arranged in the volume in order to partition off a predefined measured volume from the volume. | 01-28-2010 |
20100015602 | REVERSE TRANSCRIPTION AND AMPLIFICATION OF RNA WITH SIMULTANEOUS DEGRADATION OF DNA - The invention relates to a method for processing RNA, in particular, a RNA reaction method and kits for carrying out said RNA reaction method. | 01-21-2010 |
20090317906 | GENE SILENCING USING SENSE DNA AND ANTISENSE RNA HYBRID CONSTRUCTS COUPLED TO PEPTIDES FACILITATING THE UPTAKE INTO CELLS - The present invention generally relates to gene silencing using sense DNA (sDNA)-antisense RNA (aRNA) hybrids wherein the sense DNA strand is coupled to a peptide which facilitates the uptake of the hybrid into cells. | 12-24-2009 |
20090306177 | Modulation of Immunostimulatory Properties of Short Interfering Ribonucleic Acid (Sirna) by Nucleotide Modification - Double-stranded short interfering ribonucleic acid (siRNA) are modified to reduce or eliminate their immunostimulatory effect without significantly affecting their gene silencing effect. Modified siRNA include one or more 2′ sugar modifications and, optionally, internucleotide linkages on the sense strand. Compositions containing the modified siRNA and methods of making and using the modified siRNA are disclosed. New and previously characterized siRNA can be synthesized to incorporate modifications according to the invention. | 12-10-2009 |
20090305392 | DEVICE FOR PROCESSING SAMPLES - Device for sample processing, particularly sample conditioning as well as for the preparation and/or optionally for implementing a sequential process for an analyte from a biological sample, said device comprising a module for receiving and/or outputting at least one sample vessel or process vessel, a module for transporting a process vessel, a module for sample conditioning and a module for initiating a sequential process for an analyte. The modules are divided into at least two units that each possesses a control system, and which are connected through a first data bus. | 12-10-2009 |
20090299047 | Method for Activating a Nucleic Acid for a Polymerase Reaction - The present invention concerns a method for activating a nucleic acid for a polymerase reaction with the steps: (a) Heating a nucleic acid to a temperature of 55° C. to 80° C., (b) cooling the nucleic acid to a temperature at which a polymerase shows no substantial decrease in activity, and (c) starting the polymerase reaction by the addition of a heat-labile polymerase to the nucleic acid. | 12-03-2009 |
20090212233 | MALDI CHIP HOLDER - The invention relates to a holder for a MALDI chip, having a broken corner contour, comprising a first and second respectively accessible bearing surface and a moveable fixing means which interacts with the broken corner contour and which pre-tenses the MALDI chip against the bearing surfaces. The invention also relates to a MALDI chip and a system consisting of the holder and the MALDI chip. | 08-27-2009 |
20090202998 | METHOD FOR THE EXTRACTION OF BIOMOLECULES FROM FIXED TISSUES - The present invention relates to a method for treating a fixed biological sample, comprising the steps of the method: i) provision of a fixed biological sample, ii) contacting the fixed biological sample with an aqueous solution comprising at least one nucleophilic reagent, and iii) heating the biological sample contacted with the aqueous solution. The invention also relates to the biological sample obtainable by this method, to the use of a nucleophilic reagent for the treatment of a fixed biological sample, to a kit for isolating a biomolecule from a fixed biological sample, to the use of this kit, and to a method for the treatment of a disease. | 08-13-2009 |
20090176296 | PROCESS FOR ISOLATING NUCLEIC ACIDS - The present invention relates to a process for isolating total nucleic acid from a nucleic acid-containing sample and a kit for carrying out said process. More specifically, it relates to the isolation of RNA from a nucleic-acid containing sample. | 07-09-2009 |
20090117622 | MIXTURE OF REVERSIBLY INHIBITED ENZYMES - The invention relates to composition or a kit containing an enzyme that is reversibly inhibited by means of a chemical modification and an enzyme which is reversibly inhibited using non-covalent binding, the use of a mixture of enzymes reversibly inhibited in such a manner for processing or multiplying polynucleotides, and a method for specifically amplifying DNA by simultaneously using both types of reversibly inhibited enzymes. | 05-07-2009 |
20090081802 | METHOD FOR ENRICHING SHORT-CHAIN NUCLEIC ACIDS - The present invention relates to a method for enriching nucleic acids with a length of not more than 300 nucleotides | 03-26-2009 |
20090063290 | System and Method Utilizing A Customer Relationship Management Software Application To Convert A Price Quote Into An Electronic Shopping Cart - A customer requests a price quote for certain goods or services. The requisite information is entered into a customer relationship management (CRM) software application, which is capable of sending a price quote to the customer. The CRM application is provisioned with a feature that creates an e-mail containing a weblink to an electronic shopping basket, along with other information pertaining to the price quote. Once finalized, the e-mail is sent to the customer who had requested the price quote. If the customer then activates the weblink, the web site application displays the electronic shopping cart to the customer, and gives the customer the opportunity to conclude the sale. This makes it easy for the customer to purchase the goods or services in the shopping cart, without further the delay. For the vendor, it increases the likelihood of converting a price quote into a sale. | 03-05-2009 |
20090047674 | METHOD FOR NUCLEIC ACID ISOLATION AND AMPLIFICATION - The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof. | 02-19-2009 |
20090018323 | METHOD FOR EXTRACTING NUCLEIC ACID FROM BLOOD - The present invention relates to an improved method for isolating nucleic acids, particularly genomic desoxyribonucleic acid (DNA) from blood. | 01-15-2009 |
20090010871 | Method for Production of a Transformed Cell - The invention relates to a method for incorporation of a biomolecule into a cell including the method steps: i) production of a complex from a biomolecule, preferably a nucleic acid and a polymer which may be obtained by reaction of an amine monomer having at least two amine groups with an epoxide monomer having at least two epoxide groups and ii) incorporation of the biomolecule into a cell bx bringing the cell into contact with the complex. The invention further relates to the transformed cell obtained by said method, the use of a particular polymer for incorporation of a biomolecule into a cell, a kit for incorporation of a biomolecule into a cell, the use of said kit, a complex made from a biomolecule and a polymer, a therapeutic composition, the use of a complex of a biomolecule and a polymer for therapeutic treatment of the human or animal being and a method for treatment of a disease by means of gene therapy. | 01-08-2009 |
20080311562 | Nucleic Acid Sequencing - A method for determining a target nucleic acid sequence, wherein the target nucleic acid sequence is comprised in a preparation comprising a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, the method comprising: | 12-18-2008 |
20080299557 | Sample Lysis and Coating of Reaction Surface - The present invention provides copolymers that facilitate nucleic acid analysis, compositions that comprise such copolymers, and methods for making or using such copolymers. | 12-04-2008 |