LEICA MICROSYSTEMS CMS GMBH Patent applications |
Patent application number | Title | Published |
20150286040 | MICROSCOPE AND A METHOD FOR EXAMINING A SAMPLE USING A MICROSCOPE - A microscope, in particular a confocal microscope, has one or more lasers for generating an illumination light for a sample and has a detection device for detected signals from the sample. The detection device includes multiple adjustable spectral detection channels for the detection of predefinable different wavelength regions, and is configured and refined in the interest of particularly versatile utilization in consideration of a wide variety of phenomena, with particularly good separation of the phenomena in the context of investigation, in such a way that multiple temporal detection windows are respectively settable for the spectral detection channels. | 10-08-2015 |
20150212306 | CONFOCAL LASER SCANNING MICROSCOPE HAVING A LASER LIGHT SOURCE TO WHICH PULSED CONTROL IS APPLIED - The present invention relates to a confocal laser scanning microscope ( | 07-30-2015 |
20150205087 | OPTICAL ARRANGEMENT AND A MICROSCOPE - An optical arrangement in a microscope includes an illumination device configured to generate an illuminating light beam extending on an illumination side. A splitting device is configured to split the illuminating light beam into at least two partial beams. A mirror arrangement is configured to reflect the partial beams into an illumination region for plane illumination of a specimen. Detection optics are arranged on a side of the illumination region facing away from the illumination side. | 07-23-2015 |
20140306098 | DETECTOR APPARATUS - A detector apparatus that is embodied to receive light and to generate electrical signals has a housing and a detector arranged in the housing. The detector includes a light sensor that is embodied to receive light and to release electrons. The light sensor is at a lower electrical potential level than the housing; and that the detector is in thermally conductive contact with the housing via an electrically insulating intermediate arrangement, the thermal conduction direction inside the housing being opposite to the light propagation direction of the light to be detected. | 10-16-2014 |
20140300958 | ARRANGEMENT FOR USE IN THE ILLUMINATION OF A SPECIMEN IN SPIM MICROSCOPY - An arrangement for use in illuminating a sample in SPIM microscopy includes an illumination objective configured to receive and focus a light strip or a quasi-light strip. The quasi-light strip is made up of a light bundle continuously moved back and forth in a light-strip plane. A deflection apparatus is configured to deflect the light strip or the quasi-light strip, after the light strip or the quasi-light strip has passed through the illumination objective, in such a way that the light strip or the quasi-light strip propagates at an angle different from zero degrees with respect to an optical axis of the illumination objective. The illumination objective and the deflection apparatus are arranged movably relative to one another. | 10-09-2014 |
20140218783 | ACOUSTO-OPTICAL TUNABLE FILTER ELEMENT - An acousto-optical filter element ( | 08-07-2014 |
20140168753 | APPARATUS AND METHOD FOR OPERATING AN ACOUSTO-OPTICAL COMPONENT - An apparatus for controlling an acousto-optical component influencing at least one of illumination light and detection light in a microscope is described. The apparatus comprises a radio-frequency generator for supplying the acousto-optical component with a radio frequency. The radio-frequency generator is configured to compensate deviations in the characteristics of the light due to temperature fluctuations in the acousto-optical component by adapting the radio frequency. The apparatus can be operated by generating a control signal for controlling the radio frequency of the radio-frequency generator; measuring the temperature of the acousto-optical component; adapting the control signal depending on the measured temperature; and sending the adapted control signal into the radio-frequency generator for compensating deviations in the characteristics of the light due to temperature fluctuations and can be used in optical coherence tomography, particularly white light interferometry, optical tweezers in lithography, and distance measurement. | 06-19-2014 |
20140153089 | DEVICE FOR FOCUSING A MICROSCOPE OBJECTIVE ON A SAMPLE - A device for focusing a microscope objective on a sample accurately with a high spatial resolution. The device has a positioning unit having a main body, an objective holder movably supported on the main body and adapted to hold the microscope objective, and an actuator for moving the objective holder along the optical axis of the microscope objective. The objective holder holds the microscope objective only at a front portion of the microscope objective facing the sample. The positioning unit includes one or more lever arms, each of which coupled at its one end via a first flexure bearing to the main body and at its other end via a second flexure bearing to the objective holder. The main body of the positioning unit is attached to a stage that carries the sample. | 06-05-2014 |
20140147884 | LASER MICRODISSECTION DEVICE AND METHOD - A laser microdissection device includes a microscope stage for carrying a specimen to be cut, a laser light source for generating a laser beam and a microscope objective for focusing the laser beam onto the specimen. An optical laser scanning device is configured to deflect the laser beam so as to move the laser beam focus in an X-Y direction perpendicular to a main axis of the microscope objective. The laser microdissection device also includes at least one of a Z displacement device configured to move the microscope stage in a Z direction, or an optical focus shifting device configured to move the focus of the laser beam in the Z direction. The Z displacement device and/or optical focus shifting device are controllable, together with the optical laser scanning device, so as to move the laser beam focus in the specimen along a three-dimensional cut line in an X-Y-Z direction. | 05-29-2014 |
20140061445 | WIDEFIELD MICROSCOPE ILLUMINATION SYSTEM AND WIDEFIELD ILLUMINATION METHOD - A widefield microscope illumination system and a method for illumination, the system having a microscope objective with an optical objective axis, an illumination light source sending widefield illumination light along illumination beam paths having corresponding illumination axes along which the illumination light penetrates into the microscope objective through illumination light entry sites located within a predetermined illumination light entry area, a spatially resolving light detector detecting detected light sent from an illuminated sample through the microscope objective along a detected light beam path, and an automatic illumination light beam path manipulation device, controlled by a control system, which is arranged in front of the microscope objective in relation to the direction of the illumination light beam path, and by which illumination light beam path manipulation device the illumination axes are automatically movable at time intervals to a plurality of illumination light entry sites. | 03-06-2014 |
20140049818 | Specimen Slide Having Reference Points - A specimen slide ( | 02-20-2014 |
20130342902 | Microscope Having an Autofocusing Device and Autofocusing Method for Microscopes - A method for autofocusing in microscopic examination of a specimen located at the focus of a microscope objective uses an autofocus beam path, the autofocus beam path being directed, via a deflection device arranged on the side of the microscope objective facing away from the specimen, toward the microscope objective, and from there onto a reflective autofocus interface in the specimen region. The autofocus beam path is reflected at the autofocus interface and directed via the microscope objective and the deflection device toward an autofocus detector. The deflection device comprises two regions spaced apart from one another in a propagation direction of the autofocus beam path. Each region reflects the autofocus beam path. The autofocus detector is arranged in a plane conjugated with the microscope objective pupil to acquire an interference pattern. The focus of the microscope is adjusted as a function of the acquired interference pattern. | 12-26-2013 |
20130342834 | CONFOCAL LASER SCANNING MICROSCOPE AND A METHOD FOR EXAMINING A SAMPLE - A confocal laser scanning microscope for examining a sample has a light source, which generates an illumination light beam, and a scanning unit which deflects the illumination light beam such that it optically scans the sample. A main beam splitter separates the illumination light beam from detection light emerging from the sample. The detection light separated from the illumination light beam passes at least partially through a detection pinhole diaphragm. At least two detector units detect the detection light passing through the detection pinhole diaphragm. An optical element is arranged in the beam direction between the detection pinhole diaphragm and the detector units and splits the detection light into at least two beam bundles and spectrally decomposes it within the beam bundles. | 12-26-2013 |
20130335818 | Scanning Microscope, and Method for Light Microscopy Imaging of a Specimen - A scanning microscope is described, having an illumination unit for emitting an illumination light beam, an objective for generating an elongated illumination focus in a specimen to be imaged, and a scanning apparatus for moving the illumination focus over a target region of the specimen to be illuminated by modifying the direction of incidence in which the illumination light beam is incident into an entrance pupil of the objective. The scanning apparatus directs the illumination light beam onto a sub-region of the entrance pupil offset from the pupil center in order to incline the illumination focus relative to the optical axis of the objective, and modifies the direction of incidence of the illumination light beam within that sub-region in order to move the illumination focus over the target region to be illuminated. | 12-19-2013 |
20130311816 | CIRCUIT AND METHOD FOR CONTROLLABLY DELAYING AN INPUT SIGNAL, AND MICROSCOPE, AND METHOD FOR CONTROLLING A MICROSCOPE - A circuit for delaying an input signal includes first and second delay units. The input signal is switched to the first delay unit which is configured to delay the input signal by k cycles of a first clock signal so as to generate a value x | 11-21-2013 |
20130307958 | CIRCUIT AND METHOD FOR CONTROLLING A MICROSCOPE - A circuit for controlling a microscope using a controller includes a slow memory configured to store control information. The controller is configured to control microscope parameters based on the control information. The circuit includes a data loader and at least two fast memories. The data loader is configured such that control information is written from the slow memory alternately into one of the at least two fast memories. The circuit also includes a multiplexer configured to permit access by the controller to one of the at least two fast memories in alternating fashion so as to read control information. The data loader is configured to control the multiplexer in such a way that a write operation by the data loader and a read operation by the controller will not occur simultaneously on the same fast memory. | 11-21-2013 |
20130307395 | SLIDE STORAGE ASSEMBLY - The invention relates to a slide storage assembly ( | 11-21-2013 |
20130306398 | Apparatus for Damping Sound in the Optical Beam Path of a Microscope, and Microscope Having a Corresponding Apparatus - An apparatus for damping sound in the optical beam path of a microscope, having an acoustic insulation housing for encapsulating a sound-emitting component, preferably a rapidly moving or oscillating beam deflection means, in particular a resonantly oscillating mirror, the housing comprising at least one optical entrance/exit opening, is characterized in that the housing, preferably the opening of the housing, is embodied and/or configured in such a way that the sound otherwise emerging from the housing is largely extinguished by destructive interference without thereby influencing the optical beam. A microscope having a corresponding apparatus is furthermore claimed. | 11-21-2013 |
20130287649 | DEVICE FOR STRETCHING SAMPLE SECTIONS - The present invention relates to a glass plate ( | 10-31-2013 |
20130259773 | RETAINER FOR SAMPLE CARRIERS OF DIFFERENT SHAPES AND SIZES - The disclosure relates to a retainer ( | 10-03-2013 |
20130229494 | MICROSCOPIC DEVICE AND MICROSCOPIC METHOD FOR THE THREE-DIMENSIONAL LOCALIZATION OF POINT-LIKE OBJECTS - A microscopic device provides three-dimensional localization of point-like objects and includes two imaging optics, each configured to image a same point-like object located in an object space into two separate image spaces as a focused light distribution. Two detector units are respectively associated with the imaging optics and configured to capture an analyzable light spot in detection points of a detection surface disposed in the respective image space. Each imaging optics includes an optical device that orients the focused light distributions obliquely to a detection axis such that, taking into account the detection point correspondence, the two light spots shift in opposite directions based on a z-position of the point-like object. An evaluation unit brings the detection points of the two detection surfaces into mutual pairwise correspondence and analyzes the two light spots so as to ascertain a lateral x-y position and an axial z-position of the point-like object. | 09-05-2013 |
20130222568 | Method and Device for Light-Microscopic Imaging of a Sample Structure - A method for light-microscopy imaging of a sample structure ( | 08-29-2013 |
20130170023 | Device for Continuous Adjustment of Spectrometer Gap Widths - A microscope ( | 07-04-2013 |
20130140437 | Method and Apparatus for Examining a Sample - A method and an apparatus for examining a sample. The apparatus has a light source for generating excitation light in pulses which occur in succession at an excitation pulse frequency, for illuminating a sample region with the excitation pulse, and having a detector for detecting the detection light emanating from the sample region. The apparatus is characterized in that, for each detected photon of the detection light, the detector generates an electrical pulse and thereby a sequence of electrical pulses, and an analog-digital converter is provided that generates a digital data sequence by sampling the sequence of electrical pulses at a sampling rate. | 06-06-2013 |
20130135717 | Microscope Illumination System and Method - The present invention relates to a microscope illumination system for switching between a first, confocal and a second, non-confocal microscope illumination mode, the system having an illumination unit that, in order to provide the first illumination mode, includes an illumination source for generating an illumination beam propagating parallel to the optical axis; a scanning mirror for deflecting the illumination beam perpendicular to the optical axis; and a scanning eyepiece and a downstream scanning tube lens for imaging the scanning mirror into the back focal plane of a microscope objective and for expanding the illumination beam, the objective focusing the illumination beam onto a specimen to be examined. In order to provide the second illumination mode, the system has a focusing lens inserted into the path of the illumination beam in such a way that the illumination beam is focused into the back focal plane of the microscope objective. | 05-30-2013 |
20130128025 | Device and Method for Acquiring a Microscopic Image of a Sample Structure - A device and a method for acquiring a microscopic image of a sample structure are described. An optic for imaging the sample structure and a reference structure is provided, as well as a drift sensing unit for sensing a drift of the sample structure relative to the optic on the basis of the imaged reference structure. The optic comprises a first sharpness plane for imaging the sample structure and at the same time a second sharpness plane, modifiable in location relative to the first sharpness plane, for imaging the reference structure. | 05-23-2013 |
20130120539 | MICROSCOPIC DEVICE AND METHOD FOR THREE-DIMENSIONAL LOCALIZATION OF POINT-LIKE OBJECTS IN A SPECIMEN - A microscopic device for three-dimensional localization of point-like objects, encompassing a detection optical system that images point-like objects, each in the form of a three-dimensional focus light distribution, into an image space; a color separation apparatus that divides the light into at least two separate light bundles of different wavelength regions; at least two image space detector units , one receiving one light bundle and the other receiving the other light bundle, each detector unit comprising a light-spot-sensing detection surface ; an evaluation unit that ascertains a lateral X-Y position and an axial Z position relative to the sharpness plane in a direction perpendicular to the sharpness plane; at least one Z-position correction value for at least one of the wavelength regions being stored in the evaluation unit, which value indicates a detection optical system longitudinal chromatic aberration in that wavelength region; and the evaluation unit correcting the Z position. | 05-16-2013 |
20130119276 | Method for Measuring the Lifetime of an Excited State in a Sample - The present invention relates to a method for measuring the lifetime of an excited state in a sample, in particular a fluorescence lifetime, and to an apparatus for carrying out such a method. First, an excitation pulse is generated and a sample region is illuminated with the excitation pulse. Then, a first digital data sequence is generated which is representative of the power-time profile of the excitation pulse, and a first switching instant is determined from the first digital data sequence. Moreover, the detection light emanating from the sample region is detected by a detector, and a second digital data sequence is generated which is representative of the power-time profile of the detection light, and a second switching instant is determined from the second digital data sequence. Finally, the time difference between the first and second switching instants is calculated. | 05-16-2013 |
20130119273 | Method and Apparatus for Illumination and Detection in RESOLFT Microscopy - A method for illumination and detection in RESOLFT microscopy using a pulsed or continuous light source for excitation light and switching light is characterized in that the excitation light ( | 05-16-2013 |
20130094078 | ILLUMINATING ARRANGEMENT FOR A MICROSCOPE - Illuminating arrangement for a microscope ( | 04-18-2013 |
20130094016 | METHOD AND APPARATUS FOR IDENTIFYING AND CORRECTING SPHERICAL ABERRATIONS IN A MICROSCOPE IMAGING BEAM PATH - A method and apparatus provide identification of a spherical error of a microscope imaging beam path in a context of microscopic imaging of a sample using a microscope having an objective. A coverslip that carries or covers the sample is arranged in the imaging beam path. A measurement beam is guided through the objective onto the sample in a decentered fashion that is outside an optical axis of the objective. The measurement beam is reflected at an interface of the coverslip with the sample and the reflected measurement beam is guided through the objective onto a detector. An intensity profile of the reflected measurement beam is detected with the detector and a presence of a spherical error from the intensity profile is determined qualitatively and/or quantitatively. | 04-18-2013 |
20130044370 | DEVICE AND METHOD FOR SCANNING AN OBJECT AND A MICROSCOPE - The invention relates to a device for scanning an object comprising a focusing lens system ( | 02-21-2013 |
20130043378 | DETECTOR APPARATUS - A detector apparatus is configured to receive light and generate electrical signals. The detector apparatus includes a housing, a detector disposed in the housing and a cooling component disposed in the housing. The cooling component electrically insulates the detector with respect to the housing or is part of an insulator electrically that insulates the detector with respect to the housing. | 02-21-2013 |
20130043377 | DETECTOR APPARATUS - A detector apparatus is configured to receive light and generate electrical signals. The detector apparatus includes a light sensor having a light incidence side and a cooling component. The cooling component is in direct contact with at least one of the light sensor, on the light incidence side, or a substrate carrying the light sensor. | 02-21-2013 |
20130043376 | DETECTOR DEVICE - A detector device is configured to receive light and generate electrical signals. The detector device includes a housing, a detector disposed in the housing and a cooling component disposed in the housing. The cooling component is at least one of: positioned so as to have a light path extend through the cooling component, where the light path is defined by light that is received for detection; designed so as to include a thermally conductive, electrically insulating intermediate element; and disposed in direct contact a light sensor of the detector and/or a substrate bearing the light sensor. | 02-21-2013 |
20130038875 | DEVICE AND METHOD FOR DISTRIBUTING ILLUMINATION LIGHT AND DETECTED LIGHT IN A MICROSCOPE - A device for distributing illumination light and detected light in a microscope includes a distributor optic configured to guide illumination light onto a sample and guide detected light proceeding from the sample onto a detector. The distributor optic includes a polarization unit disposed in a first light path and configured to convert the illumination light directed onto the sample into a first polarization state, a beam splitter disposed in the first light path and having the polarization dependence so as to guide the converted illumination light onto the sample, a first portion of the detected light back into the first light path, and a second portion of the detected light into a second light path separated from the first light path. A beam combiner is configured to combine the first portion and the second portion of the detected light and guide the first portion and second portion onto the detector. | 02-14-2013 |
20130034913 | Method for Investigating a Specimen Containing Fluorescing Dyes with the Aid of a Microscope - In order to investigate a specimen ( | 02-07-2013 |
20130033744 | Sample Holder For A Microscope - A sample retainer for a microscope is described, comprising a sample stage ( | 02-07-2013 |
20130033734 | Device for Variable Deflection of Light | 02-07-2013 |
20130032699 | DEVICE AND METHOD FOR COUNTING PHOTONS - A device for counting photons includes a detector unit that is configured to generate an detected signal. A switching unit is configured to be impinged upon by the detected signal and to trigger a switching state for each detection pulse so as to generate a state signal. A sampling unit is configured to sample the state signal at a predetermined sampling frequency. A serial-parallel converter unit is configured to parallelize the serially generated sampled data by grouping successive sampled data into a sampled data packet. An evaluation unit is configured to evaluate the binary values of sampled data packets so as to identify a partial counter result indicating the number of switching state changes occurring in the switching unit, and to add partial counter results identified in individual clock cycles. | 02-07-2013 |
20130027770 | Microscope Illumination Method and Microscope - The invention relates to a microscope illumination method, to a microscope illumination set, comprising a white light LED ( | 01-31-2013 |
20130027769 | Microscope Illumination Method and Microscope - The present invention relates to a method for illuminating a sample ( | 01-31-2013 |
20130021665 | CHANGING APPARATUS FOR A MICROSCOPE - A changing apparatus for a microscope ( | 01-24-2013 |
20130015331 | DEVICE AND METHOD FOR DETECTING LIGHTAANM Birk; HolgerAACI MeckesheimAACO DEAAGP Birk; Holger Meckesheim DEAANM Seyfried; VolkerAACI NusslochAACO DEAAGP Seyfried; Volker Nussloch DE - A device for detecting light includes at least one silicon photomultiplier (SiPM) having an array of a plurality of single-photon avalanche diodes (SPADs), the array being larger in area than an incident light. The device is configured so as to at least one of activate and analyze only the SPADs upon which a specific minimum intensity of light impinges. | 01-17-2013 |
20130010367 | Microscope Objective - Described is a microscope objective ( | 01-10-2013 |
20130010340 | DEVICE FOR SCANNING AN OBJECT, METHOD FOR OPERATING THE DEVICE AND SCANNING MICROSCOPE - The invention relates to a device for scanning an object comprising a carrier body ( | 01-10-2013 |
20130003172 | SCANNING MICROSCOPE AND METHOD FOR OPTICALLY SCANNING ONE OR MORE SAMPLES - A device in the form of a scanning microscope, a device in the form of a structural unit for a microscope and a method and a device for optically scanning one or more samples. A device in the form of a scanning microscope has a light source ( | 01-03-2013 |
20120327209 | Method and Light Microscopy Apparatus for Producing an Image of a Sample - A sequence of individual images is acquired by imaging the sample through imaging optics onto an image sensor. For the acquisition of each individual image, the sample is provided with a marker pattern, in which individual markers can be imaged in the form of spatially separable light distributions through the imaging onto the image sensor. The centroid positions of the light distributions are determined and superimposed to form a complete image of the sample. According to the present invention, an image-drift-inducing temperature value (ΔT | 12-27-2012 |
20120320438 | Scanning Microscope and Method for Light-Microscopic Imaging of an Object - A scanning microscope includes a light source, illumination optics, and a scanning device for moving the illumination focus across a target region and doing so by varying the direction of incidence in which the illuminating beam enters an entrance pupil of the illumination optics. To incline the illumination focus relative to the optical axis of the optics, the scanning device directs the illuminating beam onto a portion of the entrance pupil that is offset from the center of the pupil and, in order to move the illumination focus across the target region, the scanning device varies the direction of incidence of the illuminating beam within said portion. An observation objective is provided which is spatially separated from the illumination optics and disposed such that its optical axis (O | 12-20-2012 |
20120320184 | Microscope and Method for Fluorescence Imaging Microscopy - A microscope for fluorescence imaging microscopy, in particular for wide-field fluorescence microscopy, including a pulsed light source ( | 12-20-2012 |
20120305803 | METHOD AND DEVICE FOR SETTING A SUITABLE EVALUATION PARAMETER FOR A FLUORESCENCE MICROSCOPE - A method for setting an evaluation parameter for a fluorescence microscope includes exciting dye particles in a sample to fluoresce and detecting fluorescent light from the particles. A graphical representation of a distribution of the fluorescent light is determined and a signal is generated for use in displaying the graphical representation on a display unit. Each subregion of the graphical representation is associated with a comparison value that is representative of a light quantity in the subregion. A predefined threshold is used as an evaluation parameter and compared to the comparison values. The subregions having a comparison value that is greater than the threshold value are marked on the display unit with predefined markings. The threshold value is changed and the comparison values are compared to the changed threshold value. The marked regions are defined as events and a complete image of the sample is obtained based on the events. | 12-06-2012 |
20120243079 | DEVICE FOR HOLDING FILTERS FOR MICROSCOPES - A device for holding filters for a microscope includes a filter wheel ( | 09-27-2012 |
20120236398 | Phase Filters for a Scanning Microscope - The aim of the invention is an optical device for a scanning microscope, said device enabling the focusing of a light beam largely independent of wavelengths; and thus high-resolution microscopy, in particular STED microscopy, in a wider wavelength spectrum is facilitated. At least two phase filters lie on a support. Advantageously, the support is a filter wheel or a filter slider which can be introduced into the beam path of the light beam, said beam path preferably being the beam path of the stimulating light beam in an STED microscope. Several phase filters preferablylie on the support in the shape of a matrix. The support is designed as a glass substrate on which each phase filter is applied. To achieve said aim, another position is additionally found on the support for adjustment purposes, wherein the wavefront of the light is not influenced when it passes through said position, that is, the position is an empty position on which no phase filter is found. | 09-20-2012 |
20120218631 | Pulse Combiner for the Various Spectral Colors of a Supercontinuum Laser Pulse - An illumination device ( | 08-30-2012 |
20120212790 | DEVICE AND METHOD FOR DEFLECTING A LIGHT BEAM IN TWO DIFFERENT DIRECTIONS AND SCANNING MICROSCOPE - A device for deflecting a light beam in two different directions includes a mirror and a first rotating actuator element configured to rotate about a first axis as a function of a first actuation signal. A second rotating actuator element is disposed opposite to the first rotating actuator element along the first axis and configured to rotate about the first axis as a function of a second actuation signal. A first spring element is connected to the first rotating actuator element and, off-axially with respect to the first axis at a predetermined first distance thereto, to the mirror in a rest position of the mirror. A second spring element is connected to the second rotating actuator element and to the mirror. | 08-23-2012 |
20120193513 | LASER SYSTEM FOR A MICROSCOPE AND METHOD FOR OPERATING A LASER SYSTEM FOR A MICROSCOPE - The invention relates to a laser system ( | 08-02-2012 |
20120175505 | Method and Device for Scanning-Microscopy Imaging of a Specimen - A method and a device for scanning-microscopy imaging of a specimen ( | 07-12-2012 |
20120169864 | Method for Correcting Image Distortions in a Confocal Scanning Microscope - In order to identify scan coordinate values (φ | 07-05-2012 |
20120147462 | MICROSCOPE STAGE WITH PIVOTABLE OBJECTIVE HOLDER - A microscope stage ( | 06-14-2012 |
20120147461 | MICROSCOPE STAGE - A microscope stage ( | 06-14-2012 |
20120147459 | DEVICE AND METHOD FOR THE ADJUSTED MOUNTING OF A MICROSCOPE STAGE TO A MICROSCOPE STAND - A device and method for adjusting mounting of a microscope stage ( | 06-14-2012 |
20120134017 | DEVICE FOR FOCUSING A MICROSCOPE OBJECTIVE ON A SAMPLE - A device for focusing a microscope objective on a sample accurately with a high spatial resolution. The device has a positioning unit having a main body, an objective holder movably supported on the main body and adapted to hold the microscope objective, and an actuator for moving the objective holder along the optical axis of the microscope objective. The objective holder holds the microscope objective only at a front portion of the microscope objective facing the sample. The positioning unit includes one or more lever arms, each of which coupled at its one end via a first flexure bearing to the main body and at its other end via a second flexure bearing to the objective holder. The main body of the positioning unit is attached to a stage that carries the sample. | 05-31-2012 |
20120130670 | System and Method for Computer-Controlled Execution of at Least One Test in a Scanning Microscope - The invention relates to a method and a system for central computer controlled execution of at least one test run in a scanning microscope, particularly a confocal microscope, wherein at least one first software module of an application software is tested. The invention achieves the aim by a network made of individual scanning microscope clients and a central server. The clients can be contacted via a network interface and are administered in a central directory in the server. The application software for the individual components of a scanning microscope is made of individual software modules, each associated with a potential test. In order to be able to perform the various tests, the scanning microscope clients have been equipped on the hardware side with additional sensors and components that allow various operating parameters to be determined. | 05-24-2012 |
20120120500 | Optical Element for Distributing Light - An optical element ( | 05-17-2012 |
20120099190 | SPIM Microscope with a sequential light sheet - A SPIM-microscope (Selective Plane Imaging Microscopy) having a y-direction illumination light source and a z-direction detection light camera. An x-scanner generates a sequential light sheet by scanning the illumination light beam in the x-direction. By an illumination optics having a zoom optics that is provided in the beam path of the illumination light beam the focal length of the illumination light beam can be varied. | 04-26-2012 |
20120098949 | SPIM MICROSCOPE WITH A STED LIGHT SHEET - A STED-SPIM-microscope (Selective Plane Imaging Microscopy) having a y-direction illumination light source and a z-direction detection light camera. An x-scanner generates a sequential light sheet by scanning the illumination light beam in the x-direction. By optionally turning on a STED deactivation light beam the light sheet can optionally be made thinner and therefore the optical resolution can be increased. | 04-26-2012 |
20120092479 | METHOD AND APPARATUS FOR THE MICROSCOPIC IMAGING OF A SAMPLE STRUCTURE - A method and an apparatus for imaging a sample structure ( | 04-19-2012 |
20120086795 | MICROSCOPE ILLUMINATION SYSTEM, MICROSCOPE AND OBLIQUE INCIDENT ILLUMINATION METHOD - A microscope illumination system includes a first light source configured to provide light along an optical axis and an aperture device including an aperture wheel that is rotatable about an axis of rotation. The aperture wheel includes a plurality of apertures of varying size disposed circumferentially on the aperture wheel. The aperture wheel is rotatable so as to dispose each of the apertures in a position that is centered on the optical axis so as to generate an illumination beam path that extends in a centered relationship with respect to the optical axis, and so as to dispose each of the apertures in a position that is off-center from the optical axis and that is within a defined region around the optical axis so as to generate an illumination beam path that extends off-center from the optical axis for oblique incident illumination. | 04-12-2012 |
20110317260 | Autofocusing Device for Microscopes and Suitable Autofocus Aperture Stops - The present invention relates to an autofocus aperture stop ( | 12-29-2011 |
20110304900 | ACOUSTO-OPTICAL SYSTEM, MICROSCOPE AND METHOD OF USE OF THE ACOUSTO-OPTICAL SYSTEM - An acousto-optical system is described comprising at least one acousto-optical element having at least one transducer that is attached to a crystal, a driver unit for generating at least one acoustic signal for driving acousto-optical elements modifying light transmitted through the acousto-optical element and comprising at least one digital data processing unit, at least one digital-to-analog converter transforming the digital combination signal into an initial analog driver signal, and an amplifier for amplifying the initial analog driver signal to become said analog electronic driver signal. Further, a microscope and a method of operating the acousto-optical element is are described. Various objectives are achieved like more flexibility, real time compensation for non-linearity and reducing the number, size, costs and energy consumption of electronic components. | 12-15-2011 |
20110299156 | METHOD FOR FCS MEASUREMENTS - A method for conducting FCS measurements includes providing a sample volume, emitting a target light having a first wavelength from a target light source, and marking an FCS volume in the sample volume with the target light by directing the target light onto the sample volume. An illuminating light having a second wavelength is emitted from an illuminating light source, the second wavelength being different than the first wavelength, and the illuminating light is directed onto the sample volume. | 12-08-2011 |
20110273768 | TUNABLE MULTIPLE LASER PULSE SCANNING MICROSCOPE AND METHOD OF OPERATING THE SAME - A tunable multiple laser pulse scanning microscope and a method of operating the same is described, applying two pulsed laser beams with distinct wavelengths incident on a scanning spot of a sample to be imaged simultaneously or at a specific time delay. The microscope comprises at least two pulsed laser light sources emitting laser light of distinct wavelengths, an acousto-optic tunable filter (AOTF) for tuning at least one of the laser pulses, a delay stage provided upstream of the AOTF, and an actuator for moving delay stage depending on the time delay. As a result, the wavelength of at least one type of pulses is tuned, and the delay between at least two pulses of distinct wavelengths is adjusted. | 11-10-2011 |
20110226963 | METHOD AND APPARATUS FOR PERFORMING MULTIPOINT FCS - A method of performing fluorescence correlation spectroscopy with a fluorescence microscope includes selecting an illumination area of a sample, generating an illumination light beam and splitting the illumination light beam into at least three partial beams. The partial light beams are focused onto the selected illumination area using a microscope optical system of the fluorescence microscope so as to excite fluorescent dye particles in the illumination area to fluoresce. Fluorescent light emitted by the dye particles is detected and at least one diffusion coefficient representative of a diffusibility of the fluorescent dye particles is determined based on the detected fluorescent light. | 09-22-2011 |
20110222147 | APPARATUS AND METHOD FOR SIMULTANEOUS FLUORESCENCE EXCITATION (2-WAVELENGTHS-IR) - An apparatus in a simultaneous fluorescence excitation microscope is described, allowing simultaneous fluorescence excitation by light of at least two different wavelengths. The apparatus has a laser light source generating a light beam. A first beam splitter splits the light beam into a first partial light beam and a second partial light beam. A wavelength converter converts the wavelength of the first partial light beam. A microscope optical system into which the first partial light beam and the second partial light beam are coupled directs the two partial light beams onto an object to be examined. | 09-15-2011 |
20110222054 | Apparatus and Method for Multi-Modal Imaging in Nonlinear Raman Microscopy - An apparatus for the microscopic examination of an object has an illumination system which generates a first illumination light beam and a second illumination light beam. A first polarization filter ( | 09-15-2011 |
20110149290 | METHOD FOR EXAMINING AN OBJECT WITH THE AID OF A MICROSCOPE AND A MICROSCOPE FOR EXAMINING AN OBJECT - A microscope for examining an object includes a laser light source generating pulsed light so as to illuminate the object. A measuring system including a detector is adapted to detect detection light coming from the object and the measuring system generates a measurement signal based on the detection light. The microscope includes a programmable integrated circuit including a control element and at least one of a first delay element and a second delay element. The control element is configured to generate a first control signal adapted to control the detector and the measuring system. The control element is further configured to generate a second control signal adapted to control the laser light source. The first and second delay elements are configured to delay the first and second control signals, respectively. | 06-23-2011 |
20100315707 | Objective Changer - An objective changer for a microscope is described, comprising a changing device for transferring at least a first objective held in a first objective holder from a first stand-by position along a first transfer path into an operating position where the first objective defines an optical axis and a second objective held in a second objective holder from a second stand-by position along a second transfer path into an operating position where the second objective defines an optical axis. The first and second transfer paths differ from each other but both extend substantially perpendicular to the optical axis at least within a first area of the first transfer path and a first area of the second transfer path. | 12-16-2010 |
20100309546 | OBJECTIVE CHANGER AND A MICROSCOPE - An objective changer for a microscope includes a changing device configured to pendulously swing each of a plurality of objectives into a respective operating position near a focal position. | 12-09-2010 |
20100271695 | ARRANGEMENT FOR ANALYZING MICROSCOPIC AND MACROSCOPIC PREPARATIONS - A confocal scanning microscope for examining microscopic and macroscopic objects is described. The microscope comprises: a scanning optical system having optical elements imaging the light generated by a laser onto an object to be examined; an objective provided in a working distance of at least 0.4 inches from an object holder; and a zoom optical system that is connected to the scanning optical system such that the light generated by the laser passes first through the scanning optical system, then through the zoom optical system, and is then imaged through the objective onto the object. This microscope achieves that also macroscopic objects can be viewed at a high resolution. | 10-28-2010 |
20100232011 | APPARATUS AND METHOD FOR OPERATING AN ACOUSTO-OPTICAL COMPONENT - An apparatus for controlling an acousto-optical component influencing at least one of illumination light and detection light in a microscope is described. The apparatus comprises a radio-frequency generator for supplying the acousto-optical component with a radio frequency. The radio-frequency generator is configured to compensate deviations in the characteristics of the light due to temperature fluctuations in the acousto-optical component by adapting the radio frequency. The apparatus can be operated by generating a control signal for controlling the radio frequency of the radio-frequency generator; measuring the temperature of the acousto-optical component; adapting the control signal depending on the measured temperature; and sending the adapted control signal into the radio-frequency generator for compensating deviations in the characteristics of the light due to temperature fluctuations and can be used in optical coherence tomography, particularly white light interferometry, optical tweezers in lithography, and distance measurement. | 09-16-2010 |
20100214654 | APPARATUS FOR THE DETECTION OF LIGHT IN A SCANNING MICROSCOPE - A light detector for use in a line scanning microscope and a microscope comprising such a light detector are described. The light detector comprises—a line array of avalanche semiconductor detectors; and an electronic trigger circuit that is adapted to operate the avalanche semiconductor detectors in at least one of a Geiger mode with internal charge amplification and in a linear mode. The trigger circuit further comprises a parallel counter that is designed to read out in parallel light pulses detected by the avalanche semiconductor detectors. The parallel counter is adapted to accumulate the light pulses detected by the avalanche semiconductor detectors over a preset counting time. | 08-26-2010 |
20100171946 | MICROSCOPE AND METHOD FOR TOTAL INTERNAL REFLECTION-MICROSCOPY - A microscope for total internal reflection microscopy. The microscope includes at least one light source configured to provide an illumination light to an illumination beam path for an evanescent illumination of a specimen so as to reflect the illumination light at an interface to the specimen or a specimen cover so as to return reflection light into the illumination beam path, an objective through which the illumination light and detection light are directable, a detection device, and a coupling device. The coupling device includes a mirror disposed in the illumination beam path. The mirror has a reflecting surface and a hole, the hole being configured to pass the illumination light there through so as to couple the illumination light into the illumination beam path. The reflecting surface is configured to couple out at least a part of the reflection light and to direct the coupled-out reflection light to the detection devices so as to enable determination from a beam pat of the coupled-out reflection light, at least one of a quantifiable parameter and a qualifiable parameter of at least one of the evanescent illumination and an evanescent field created in the specimen. | 07-08-2010 |
20100160613 | METHOD FOR HIGH SPATIAL RESOLUTION STOCHASTIC EXAMINATION OF A SAMPLE STRUCTURE LABELED WITH A SUBSTANCE - A method for high spatial resolution stochastic examination of a biological sample structure labeled with a labeling substance is described. The method comprises providing a biological sample structure; choosing such a labeling substance that has molecules present in a first state and in a second state, and the first and second states differ from one another in at least one photophysical property such that there is sufficient probability that one portion of the molecules of the substance will be in the first state and another portion of the molecules will be in the second state and within which labeling substance a change of the state of the molecules can occur spontaneously between the two states in both directions; and labeling the biological sample structure with the substance. | 06-24-2010 |
20100134793 | OPTICAL EVALUATION METHOD BY MEANS OF LASER PULSES AND CORRESPONDING APPARATUS - An optical evaluation method and an apparatus for performing said method are described. First laser pulses of a first type and second laser pulses of a second type that differs from the first type are sent onto a sample to be examined. The sample is hit with first incident light from the two laser pulses in at least one manner of simultaneously, within a very short time lag between the two laser pulses, and a time-correlated manner of the two laser pulses, thereby generating a first optical signal, and hit with second incident light from the two laser pulses, thereby generating a second optical signal. The generated first and second optical signals are detected with at least one detector; and an electronic difference between the first and second optical signals is generated. | 06-03-2010 |
20100124788 | METHOD FOR HIGH SPATIAL RESOLUTION EXAMINATION OF A SAMPLE STRUCTURE LABELED WITH A SUBSTANCE - A method for high spatial resolution examination of a sample structure includes providing a biological structure as the sample structure with a substance capable of being converted from a first state to a second state. The first and second states of the substance differ in at least one photophysical property. The sample structure is labeled by binding a suitable protein tag including a fluorogen activating protein (FAP) to the sample structure or by expressing the protein tag and the sample structure together as a fusion protein. Then the protein tag is bound to the substance. | 05-20-2010 |
20100122588 | DEVICE AND METHOD FOR PROVIDING A PREDETERMINABLE CONCENTRATION OF AT LEAST ONE COMPONENT IN A LIQUID MEDIUM - A method and a device for providing a predeterminable concentration of at least one component in a microscopic sample liquid medium are described. The device includes a feeding device for the at least one component. Measurement data are determined, measuring a predeterminable parameter using a microscopic method. The concentration of the at least one component is adjusted or controlled via the feeding device based on the basis of measurement data. | 05-20-2010 |
20100118394 | MICROSCOPE FOR CONVENTIONAL FLUORESCENCE MICROSCOPY AND TOTAL INTERNAL REFLECTION MICROSCOPY - A microscope for conventional fluorescence microscopy (epi-fluorescence) and for total internal reflection microscopy, comprising at least one light source ( | 05-13-2010 |
20100103253 | METHOD AND MICROSCOPIC SYSTEM FOR SCANNING A SAMPLE - A method for scanning a sample using an electrically or electronically controllable microscope, includes scanning the sample so as to generate a plurality of images of the sample, each of the plurality of images corresponding to at least one of a different region of the sample and a different time. The microscope is controlled via a control computer during the scanning. The plurality of images are analyzed using at least one second computer connected via a network. The plurality of images are classified and/or the scanning is influenced based on the analyzing. | 04-29-2010 |
20100073757 | OPTICAL ARRANGEMENT AND METHOD FOR CONTROLLING AND INFLUENCING A LIGHT RAY - An optical arrangement and a related method for operating this optical arrangement are suggested, particularly in microscopes, for use as at least one of a main beam splitter and a beam combiner. One or more light beams can be coupled into the arrangement and at least one of the light beams that were coupled in can be coupled out again after having passed through the optical arrangement. In the path of the coupled in light beams at least one controllable microstructured element is provided, allowing to switch beam paths within the optical arrangement. This allows controlling or influencing the one or more light beams that are coupled out. | 03-25-2010 |
20100055296 | COATING INSTALLATION COMPRISING A RADIO DEVICE AND METHOD FOR CONTROLLING AN ACTUATOR OR A HEATER - A deposition apparatus and methods for controlling an actuator and a heating system in a deposition apparatus are proposed. The deposition apparatus comprises a deposition chamber and at least one substrate holder movably arranged within the deposition chamber for holding substrates to be coated. The deposition apparatus shall further comprise radio equipment having at least a first radio device which is connected to the substrate holder and a second radio device which is arranged at least partially outside the deposition chamber. Between the first radio device and the second radio device a radio link is to be established at least temporarily for transferring information. | 03-04-2010 |
20100053725 | ACOUSTO-OPTICAL COMPONENT - An acousto-optical component is suggested, in particular for use in the beam path of an optical arrangement, preferably of a microscope, with a crystal for the passage of light. Sound waves are generated via an electrical transducer and passed through the crystal. The sound waves passing through the crystal affect the optical properties of the crystal and therefore the light passing through the crystal. For exerting a variable influence on the light passing through the crystal, the sound waves passing through the crystal, or the acoustic field in the crystal, are/is variable. | 03-04-2010 |
20100053719 | OPTICAL SYSTEM FOR MERGING A FIRST AND A SECOND PARTIAL IMAGE BEAM, EACH PROCEEDING FROM A SPECIMEN, INTO A RESULTANT IMAGE BEAM - An optical system is described for merging a first and a second partial image beam emanating from a specimen into a resultant image beam allowing modification of the areal proportion of the respective first or second partial image beam in the resultant image beam. A stop arrangement comprises at least a first and a second movable stop element each comprising at least one stop region adapted to be brought into a working position with the first or second partial image beam. Movably arranged connecting means for connecting the two stop elements are provided to modify the respective areal proportions of the partial image beams in the resultant image beam by movement of the connecting means. | 03-04-2010 |
20100027109 | IMMERSION OBJECTIVE, APPARATUS FOR FORMING AN IMMERSION FILM AND METHOD - An immersion objective for microscopic investigation of a specimen is provided wherein an outer lens is disposed in an objective body. A delivery device including a cap that is disposed over the objective body so as to form a space adapted to receive an immersion liquid is also provided. The cap is open in a region of the outer lens so as to form a gap with the outer lens. The cap includes at least one connector configured to provide a continuous supply of the immersion liquid to the space so that the immersion liquid emerges through the gap to a region between the outer lens and at least one of the specimen and a specimen slide. | 02-04-2010 |
20100006772 | METHOD AND APPARATUS FOR HIGH-RESOLUTION OPTICAL SCANNING OF A SAMPLE - A method and an apparatus are suggested for high-resolution optical scanning, particularly in a laser scanning fluorescence microscope. A sample to be scanned comprises a first and a second substance that are switchable into a first and second energy state. In the scanning process, excitation, de-excitation and detection for the first substance is carried out at a different point in time than for the second substance. This achieves a high spatial resolution beyond the diffraction limit while at the same time a high level of information is provided with physically simple and economical means. | 01-14-2010 |
20090323058 | LASER MICROSCOPE WITH A PHYSICALLY SEPARATING BEAM SPLITTER - The invention proposes a method for imaging at least one microscopic property of a sample and an apparatus with which the proposed method can be carried out. In the method, at least one coherent illumination light with at least one illumination wavelength is produced by means of at least one light source. The illumination light is imaged onto at least one region on or within the sampled. Detection light emitted by the sample is split at least partially into incoherent detection light and into coherent detection light by means of at least on physically separating beam splitter. The coherent detection light is at least partially separated from the coherent illumination light by at least one beam-splitter element. The coherent detection light is detected. The proposed method can be used in particular for investigating the sample by means of coherent anti-Stokes-Raman scattering. | 12-31-2009 |
20090213456 | Illumination Module for Evanescent Illumination and Microscope - A microscope with a light source that produces an illumination light beam for evanescently illuminating a sample includes an adjustment mechanism with which the polarization of the illumination light beam may be changed. | 08-27-2009 |
20090195867 | INVERTED MICROSCOPE - An inverted microscope for the high-contrast imaging of objects includes multiple objectives configured to be disposable in an imaging beam path. Each objective has a respective objective pupil associated therewith. A lens system is also provided for generating respective intermediate images of the respective objective pupils. A modulator disposing device, which includes a plurality of modulators, is configured to dispose the modulators at predetermined respective locations on an optical axis corresponding to the locations of the respective intermediate images of the respective objective pupils so as to contrast an object image. | 08-06-2009 |
20090160994 | SYSTEM FOR RECORDING AND REPRESENTING IMAGES OF PREPARATIONS - A system for acquiring and displaying images of preparations is provided. The system includes a first and second illumination device, each including an illumination source defining a respective illumination beam path amid configured to emit light to illuminate the preparation. A camera and a display for displaying a portion of the preparation are further provided. The preparation can be placed on a stage that is movable in x and y coordinate directions. The frame is also provided with a combining device configured to combine the first imaging beam path and the second imaging beam path, and direct them to the camera. | 06-25-2009 |
20090034066 | APPARATUS FOR ADJUSTING A MICROSCOPE STAGE - An apparatus for adjusting a microscope stage. The microscope stage of the apparatus defines a plane on which an object to be examined can be placed. The apparatus further includes a first shaft and a second shaft rotationally coupled to the first shaft. The microscope stage is guided between the first and second shafts such that a rotational movement of at least one of the first and second shafts has a direct effect on the microscope stage. | 02-05-2009 |
20080304146 | Microscope System for Fcs Measurements - A microscope system for conducting FCS measurements. The system includes an illuminating light source configured to emit an illuminating light at an illuminating wavelength. A target light source is provided and configured to emit a target light for marking an FCS volume in a sample volume at a target wavelength. The target wavelength differs from the illuminating wavelength. The system further includes a plurality of optical elements configured to direct the illuminating light and the target light onto the sample volume. | 12-11-2008 |
20080266659 | Lens for evanescent wave illumination and corresponding microscope - The invention relates to a lens ( | 10-30-2008 |
20080198448 | FLUORESCENCE MICROSCOPE HAVING AN ILLUMINATION DEVICE - An arrangement for fluorescence microscopic examination of specimens includes: a fluorescence microscope; an illumination device that includes: a housing including an interface configured to optically couple the housing and the fluorescence microscope; a plurality of light-emitting diodes disposed in the housing; a respective collector disposed downstream of each of the light emitting diodes and configured to generate a directed light flux; and at least one dichroic splitter disposed in the housing, the at least one splitter and the light-emitting diodes being spatially disposed with respect to one another so that the directed light fluxes are combinable via the at least one splitter into a common illumination beam path directed onto the interface; and a logical control device common to the fluorescence microscope and the illumination device. | 08-21-2008 |