GE Healthcare Bio-Sciences Corp Patent applications |
Patent application number | Title | Published |
20150299641 | TEMPERATURE CONTROLLED SUPPORT SURFACES FOR SINGLE USE FLEXIBLE WALL SYSTEMS - Disclosed is a heat exchange module for use in a chemical, pharmaceutical or biological reactor system, the module configured to be disposed in the reactor system having a flexible single use container, and including at least one thermally conductive surface adapted to contact the flexible single use container to facilitate heat transfer, and a fluid circulation path through which a heat exchange fluid can be circulated. | 10-22-2015 |
20150298829 | FLEXIBLE BAG - A flexible bag and a method for filling a flexible bag which is collapsed when empty, wherein at least one fastening means is provided on a surface of the flexible bag such that at least two upper parts of the flexible bag can be held together by this at least one fastening means during a first part of filling of the flexible bag, whereby the fastening means is designed to release when the bag is filled to a certain amount such that the two upper parts being held together by the fastening means will lose contact. | 10-22-2015 |
20150053307 | DISPOSABLE VALVE AND FLEXIBLE CONTAINERS FOR PRESSURIZED BIOREACTORS - Disclosed herein are embodiments of a valve assembly for preventing dead leg spaces in a container or tubing, the embodiments including a three-way valve system for controlling back-pressure in a fluid generating device, such as a single-use high pressure bioreactor. Also disclosed is a pressurized reactor system for bioprocessing, comprising a single-use container including a flexible wall or a semi-rigid wall. | 02-26-2015 |
20140353524 | SYSTEM AND METHOD FOR DENSE-STOCHASTIC-SAMPLING IMAGING - Embodiments of the present invention are directed to imaging technologies, and, in particular, to an imaging system that detects relatively weak signals, over time, and that uses the detected signals to determine the positions of signal emitters. Particular embodiments of the present invention are directed to methods and systems for imaging fluorophore-labeled samples in order to produce images of the sample at resolutions significantly greater than the diffraction-limited resolution associated with optical microscopy. Embodiments of the present invention employ overlapping-emitter-image disambiguation to allow data to be collected from densely arranged emitters, which significantly decreases the data-collection time for producing intermediate images as well as the number of intermediate images needed to computationally construct high-resolution final images. Additional embodiments of the present invention employ hierarchical image-processing techniques to further resolve and interpret disambiguated images. | 12-04-2014 |
20140322270 | HIGH YIELD YELLOW FEVER VIRUS STRAIN WITH INCREASED PROPAGATION IN CELLS - The invention provides a an inactive, non-replicating vaccine comprising whole virion, chemically inactivated Yellow Fever virus which is inactivated using a method that ensures preservation of critical, neutralizing epitopes. The Yellow Fever virus has been adapted to propagate in cells to higher yields than the unadapted virus. The invention also provides methods for preventing Yellow Fever viral infection. | 10-30-2014 |
20140320958 | MODE-SWITCHABLE ILLUMINATION SYSTEM FOR A MICROSCOPE - Illumination system for a microscope system capable of being mode-switchable between a first and a second illumination mode, comprising one source of light for providing a collimated beam of light, at least one selector mirror capable of being positioned in at least two positions to redirect the beam of light in two different beam paths, the first beam path being a direct exit beam path wherein the selector mirror redirects the beam of light along an exit beam path to provide a first illumination mode, the second beam path is a mirror loop path comprising two or more mirrors arranged to redirect the beam of light onto the selector mirror such that it is redirected by the selector mirror a second time along the exit beam path, and wherein mirror loop path comprises at least one optical element arranged to optically alter the beam of light to provide the second illumination mode. According to one embodiment, the first illumination mode is Total Internal Reflection (TIRF) and the second illumination mode is Photokinetics (PK) illumination. | 10-30-2014 |
20140260712 | PROBE ASSEMBLY - A probe assembly for inserting a probe into a flexible or semi-rigid vessel or tubing having a distal aseptic connector for coupling to the vessel or tubing, the probe sheath comprising at least a portion that is rigid, the probe sheath extending longitudinally from the aseptic connector and having at least one inner longitudinal lumen configured to receive an elongate sensor or probe body and to permit longitudinal movement of the sensor/probe body within the probe sheath lumen, and an actuator for deploying a probe within the vessel or tubing by advancing the probe body through the aseptic connector to a position where the probe can measure at least one parameter within the vessel or tubing is disclosed herein. | 09-18-2014 |
20140219311 | ISOTHERMAL TITRATION MICROCALORIMETER APPARATUS AND METHOD OF USE - An automatic pipette assembly for an isothermal titration micro calorimetry system, comprising a pipette housing, a syringe with a titration needle arranged to be inserted into a sample cell for supplying titrant, and a linear activator for driving a plunger in the syringe, the titration needle is rotatable with respect to the housing and is provided with a stirring paddle arranged to stir fluid in the sample cell, wherein the automatic pipette assembly comprises a stirring motor for driving the rotation of the titration needle. There is also provided an isothermal titration micro calorimetry system. | 08-07-2014 |
20140177045 | FLUORESCENCE MICROSCOPES WITH POLYCHROIC MIRROR CHANGERS - Fluorescence microscopy systems with polychroic mirror changers are described. In one aspect, a polychroic mirror changer includes a polychroic-mirror array. The array includes a plate with a planar surface and a number of holes formed in the plate. The array also includes polychroic mirrors attached to the planar surface of the plate such that each polychroic mirror covers one of the holes and the reflective surface of each polychroic mirror is adjacent to and aligned with the planar surface of the plate. Each polychroic mirror is partially exposed through a corresponding hole to reflect a different subset of excitation channels of a beam of excitation light input to the changer. The polychroic-mirror array can be mounted in the changer so that when a different subset of excitation channels is selected to illuminate a specimen, the plate is moved within a single plane of motion. | 06-26-2014 |
20140158865 | MICROSCOPY INSTRUMENTS WITH DETECTOR ARRAYS AND BEAM SPLITTING SYSTEM - Microscopy instruments with detectors located on one side of the instruments are disclosed. The microscopy instruments include a splitting system and an array of detectors disposed on one side of the instrument. A beam composed of two or more separate emission channels is separated by the splitting system into two or more beams that travel along separate paths so that each beam reaches a different detector in the array of detectors. Each beam is a different emission channel and the beams are substantially parallel. | 06-12-2014 |
20140152800 | IMAGE QUALITY OPTIMIZATION OF BIOLOGICAL IMAGING - Microscopy system for biological imaging, comprising an image quality optimizer for optimizing image quality of an image of a biological sample, allowing a user to select an optimization mode from a list of functionally defined optimization modes, and wherein the system is arranged to automatically set one or more image acquisition parameters to achieve optimal imaging for the selected optimization mode based on at least one image quality parameter derived from one or more Biological Reference Objects (BRO) in the image of the biological sample selected by the user or automatically by the system. | 06-05-2014 |
20140140595 | MICROSCOPY SYSTEM AND METHOD FOR BIOLOGICAL IMAGING - Microscopy system for biological imaging, comprising an image quality monitoring system for monitoring image quality of an image of a biological sample comprising a biological object selection means arranged to let a user of the system to select one or more Biological Reference Objects (BRO) in the image of the biological sample, and an image quality evaluation means arranged to compare the signal level of image pixels of the one or more BROs with an image background signal level to calculate one or more image quality parameters for the image of the biological sample. The system is arranged to present the image quality parameters to the user as an indication of the image quality specific for the BRO(s). | 05-22-2014 |
20140109374 | AUTOMATED INSTALLATION PROCEDURE FOR A DISPOSABLE FLOW PATH - This invention provides an automated installation procedure for assembling a disposable flow path: providing a disposable flow path comprising tubing and a plurality of sensors onto a re-usable instrument; qualifying said tubing and said plurality of sensors to be on the flow path based on a standard; and determining if the tubing and the plurality of sensors comply with characteristics and performance according to limits for specifications or acceptance criteria. | 04-24-2014 |
20140076973 | METHOD FOR PREVENTING AN UNAUTHORIZED USE OF DISPOSABLE BIOPROCESS COMPONENTS - This invention provides a system and apparatus that is able to authenticate and prevent illegal manufacturing and unauthorized operation of disposable bioprocess components. This invention utilizes a ferro-electric random access memory chip (FRAM) chip to store error-correctable information on a RFID tag attached to the disposable bioprocess components, where the error-correctable information is written in sequence into the memory chip, so that the redundant information can remain in the chip when the RFID tag and disposable bioprocess component is gamma-sterilized. Also, this invention includes a method for authenticating the disposable bioprocess component that reduces liability in that a counterfeit poor quality disposable component is not used on the hardware so the user will not file an unjustified complaint. | 03-20-2014 |
20140076459 | SYSTEM AND METHOD FOR THE AUTOMATION OF COLUMN AND MEDIA PACKING - This invention provides for the fully automated, hands free, packing of chromatography columns by means of delivering a pre-calculated volume of slurry and using two different packing modalities to stop the packing when either 1) this volume has been delivered in the column, or 2) when the adapter is moved to reach a bed height corresponding to the pre-calculated volume. Thus, a chromatography column can be packed in a fully automated fashion and such a column is 1)stable and 2) has the desired performance characteristic. | 03-20-2014 |
20130171042 | ISOTHERMAL TITRATION MICROCALORIMETER APPARATUS AND METHOD OF USE - An automatic pipette assembly for an isothermal titration micro calorimetry system, comprising a pipette housing, a syringe with a titration needle arranged to be inserted into a sample cell for supplying titrant, and a linear activator for driving a plunger in the syringe, the titration needle is rotatable with respect to the housing and is provided with a stirring paddle arranged to stir fluid in the sample cell, wherein the automatic pipette assembly comprises a stirring motor for driving the rotation of the titration needle. There is also provided an isothermal titration micro calorimetry system. | 07-04-2013 |
20130129212 | METHOD FOR REDUCING IMAGE ARTIFACTS PRODUCED BY A CMOS CAMERA - The present invention relates to a system and method for reducing image artifacts for a CMOS camera used as an optical detector for a line confocal fluorescent imager. The method for reducing image artifacts in a monochromatic image comprises subtracting RGB channel offset mismatch; and correcting random row offset for each row. Preferably, the method also comprises correcting random column offset and gain. Optionally, the method also comprises clipping of defective pixels, and/or subtracting dark current. When the monochromatic image is a fluorescence image, the method also comprises a step of compressing CMOS camera noise. Also provided are a system for reducing image artifacts in a monochromatic image from a CMOS camera, as well as an image artifact reduction system for a CMOS camera-based line confocal fluorescent microscope. | 05-23-2013 |
20130118017 | MOTOR DRIVEN ROTATIONAL SAMPLING APPARATUS WITH REMOVABLE CUTTING TOOLS FOR MATERIAL COLLECTION - A motorized apparatus to simultaneously excise, retrieve, temporarily store and transport a sample of material has a hollow clamshell casing with a blended contoured grip for the fingers, a horizontal extension to eliminate slippage when held in a user's hand, and a flange bottom portion from which a removable cutting tool threaded to a drive shaft extends downwards. Within the clamshell casing an electric motor is mounted which drives, via gears, a drive shaft which rotates a cutting tool threaded to the distal end of the drive shaft. The end of the cutting tool, distal from the clamshell casing, forms a cutting edge circumscribing a circular region. An ejection rod slides reciprocally within the cutting tool between a stowed position and an expulsion position. Users core a sample from the source material by engaging contact between the cutting edge of the cutting tool and the surface of the source material, applying pressure against the surface of the source material while simultaneously activating the motor to rotate the cutting tool. The cutting region of the cutting tool passes through the source material contacting the support surface below the source material which urges the cored sample into the lumen of the cutting tool. The sample may be stored in the lumen or transported. Activation of the ejection rod moves from the stowed position towards the expulsion position displacing the temporarily stored sample from the lumen space in the cutting tool into the appropriate collection receptacle or onto a desired surface. | 05-16-2013 |
20130093873 | SYSTEM FOR SYNCHRONIZATION IN A LINE SCANNING IMAGING MICROSCOPE - A line confocal microscope system, comprising a scanning unit in the form of a mechanically driven scanning unit with a controllable a scanning trajectory and a two dimensional sensor unit operated in a rolling line shutter mode in synchronization with the scanning unit, wherein the scanning trajectory is set to have an acceleration part outside the field of view of the sensor unit. | 04-18-2013 |
20130087718 | CONFOCAL FLUORESCENCE LIFETIME IMAGING SYSTEM - A confocal fluorescence lifetime imaging (FLIM) system comprising a pulsed tuneable excitation light source arranged to provide excitation radiation to an illumination area on a target, scanning means for scanning the illumination area across the target, and at least one detector for detecting fluorescent emission from the target, wherein the pulsed light source comprises a line forming unit arranged to form a line shaped illumination area of pulsed excitation light on the target, and wherein the detector comprises shutter means arranged to operate in synchronization with the pulsed light source enabling detection of time-resolved fluorescent emission intensity from the target. | 04-11-2013 |
20130011297 | METHOD FOR STERILIZATION - The present invention relates to a method for providing a sterile connected device, especially of sensitive material, such as material not compatible with sterilization by gamma irradiation. The method comprises providing connectors and a tubing in an autoclave bag, and autoclaving the sealed autoclave bag containing the connectors and tubing for sterilization. Optionally, the connectors and tubing are assembled prior to autoclave. Also provided are sterile, connected devices sterilized according to the present method. | 01-10-2013 |
20120289690 | NUCLEIC ACID ELUTION - This invention relates to the storage on a solid matrix of genetic material, in particular DNA that has been purified prior to the application to the solid matrix. More specifically, the invention relates to a solid matrix for the storage of purified DNA, which matrix has been treated with a solution comprising plant polysaccharide inulin. One advantage of the invention is that an increased amount of DNA can be stored in the solid matrix of the present invention.” | 11-15-2012 |
20120281495 | APPARATUS FOR MIXING THE CONTENTS OF A CONTAINER - An apparatus for mixing materials is disclosed. The apparatus includes a support. A mixing container is disposed in the support, where the mixing container is configured to retain materials. A driver assembly is configured to protrude through the support and into the mixing container. The mixing container includes a paddle, the driver assembly is configured to be attached to the paddle, where the driver assembly is configured to oscillate the paddle in a back and forth direction at a set angle in order to mix the materials in the mixing container. | 11-08-2012 |
20120271042 | METHOD FOR ISOLATION OF GENOMIC DNA, RNA AND PROTEINS FROM A SINGLE SAMPLE - The invention provides systems, methods and kits for the separation and/or purification of at least two cellular components selected from genomic DNA, RNA and proteins. The method includes first lysing a biological sample to generate an aqueous solution containing the cellular components; then applying the aqueous solution to a first mineral support under conditions for genomic DNA to bind; and collecting the flowthrough which contains unbound total RNA and proteins. The method further includes applying the flowthrough to a second mineral support under conditions for RNA to bind, and collecting the flowthrough which contains proteins. The genomic DNA and total RNA bound can be eluted while the protein in the flowthrough can be further purified. Further the total RNA isolated could be used to isolate small RNA such as microRNA. | 10-25-2012 |
20120206155 | SYSTEM FOR ASSEMBLING AND UTILIZING SENSORS IN CONTAINERS - A system for measuring parameters in a container is disclosed. A container has a solution. A protective layer is deposited over at least one sensor and at least one wall of the container, where the protective layer is attached to the wall of the container to form a seal between the container and the at least one sensor. The at least one sensor is configured to have an operable electromagnetic field based on a thickness of the container and the protective layer. The at least one sensor in conjunction with a tag is in proximity to an impedance analyzer and a reader that constitute a measurement device. The at least one sensor is configured to determine at least one parameter of the solution. The tag is configured to provide a digital ID associated with the at least one sensor, where the container is in proximity to the reader and an impedance analyzer. The impedance analyzer is configured to receive a given range of frequencies from the at least one sensor based on the measured complex impedance over the given range of frequencies. | 08-16-2012 |
20110255756 | METHOD AND APPARATUS FOR SCREENING CHEMICAL COMPOUNDS - Methods and apparatus for screening large numbers of chemical compounds and performing a wide variety of fluorescent assays, including live cell assays. The methods utilize a laser linescan confocal microscope with high speed, high resolution and multi-wavelength capabilities and real time data-processing. Imaging may be done at video-rates and with use of ultraviolet illumination. | 10-20-2011 |
20110172405 | METHOD FOR SMALL RNA ISOLATION - This invention relates to a simple and rapid method for the extraction and purification of small RNA from a sample solution. Accordingly, a sample is first mixed with an organic solvent to form a mixture containing the solvent. The mixture is applied to a first mineral support for large RNA to bind. The filtrate is collected which contain unbound small RNA, and is mixed with a second organic solvent to form a second mixture containing the second solvent. This second mixture is applied to a second mineral support for small RNA to bind. After a wash step, the small RNA is eluted. Also provided is a method for the isolation of large RNA, by eluting the large RNA from the first mineral support. In addition, total protein is present in the filtrate and can be isolated by a conventional method. | 07-14-2011 |
20110166812 | SYSTEM AND METHOD FOR MONITORING PARAMETERS IN CONTAINERS - A system for measuring parameters in a container is disclosed. A system for measuring multiple parameters includes a container having a solution, at least one sensor in conjunction with a tag is in proximity to an impedance analyzer and a reader that constitute a measurement device. The at least one sensor is configured to determine at least one parameter of the solution. The tag is configured to provide a digital ID associated with the sensor, where the container is in proximity to the reader and an impedance analyzer. The impedance analyzer is configured to send and receive a given range of frequencies from the sensor, based on the parameter and calculate parameter changes based on the response. | 07-07-2011 |
20110152510 | SIMPLE LOAD AND ELUTE PROCESS FOR PURIFICATION OF GENOMIC DNA - Provided is a novel two step chromatographic purification process (load and elute) for the isolation of genomic DNA. In this method the sample is loaded on the column and the genomic DNA product is eluted directly without any intermediate wash steps. This is accomplished by utilizing a restricted access resin (i.e., lid beads), which is easy to prepare and comprised of two layers with different properties with non-functional surfaces on the outer layer. The inner layer is modified with functional groups that act as ion-exchangers. Small molecules such as RNA and proteins can enter the inner part of the resin and larger genomic DNA molecules will pass through the resin. RNA and proteins are captured in the inner layer of the restricted access resin while genomic DNA is readily eluted in the flow-through. | 06-23-2011 |
20110151552 | BIOREACTORS - The present invention relates to improvements of bioreactor bags for cell cultivation. The invention provides an inflatable bioreactor bag for cell cultivation comprised of a top and a bottom sheet of polymer material that are joined along their edges to form a sealed bag, wherein two opposing edges are formed as clamping edges to allow clamping of the bioreactor bag to a rocker type bioreactor, wherein the top sheet is at least 5% longer than the bottom sheet between the clamping edges. The bioreactor bags provided by the invention avoid formation of undesired wrinkles or creases which otherwise may lead to fatigue of the plastic and eventually fracture. | 06-23-2011 |
20110151551 | BIOREACTORS - The present invention relates to improved single-use bioreactors comprising disposable plastic bags for cell cultivation. The invention provides an inflatable bioreactor bag for cell cultivation comprised of a top and a bottom sheet of polymer material that are joined along their edges to form a sealed bag, wherein two opposing edges are formed as clamping edges to allow clamping of the bioreactor bag to a rocker type bioreactor, and wherein the bioreactor bag is provided with a wrinkle preventing structure at each end of the clamping edges. The bag avoids formation of undesired wrinkles or creases which otherwise lead to fatigue of the plastic and eventually fracture. | 06-23-2011 |
20110069905 | SYSTEM AND METHOD FOR DETECTING AND ELIMINATING ONE OR MORE DEFOCUSED OR LOW CONTRAST-TO-NOISE RATIO IMAGES - This invention, which provides a method for detecting a corruption in an image acquired from a biological sample, includes: providing at least one image of at least one cell; generating the image of the at least one cell over a period of time; determining if the at least one image of the at least one cell is corrupted; applying a wavelet transform, Fourier transform, or other frequency decomposing transform to the at least one image to decompose the at least one image into a plurality of sub-images, wherein the plurality of sub-images have a plurality of low frequency channels, a plurality of middle frequency channels and a plurality of high frequency channels; calculating a ratio based on an energy level of the plurality of low frequency channels and the plurality of middle frequency channels; and removing the at least one image of at least one cell if the at least one image is corrupted. | 03-24-2011 |
20110065151 | NUCLEIC ACID AMPLIFICATION WITH SINGLE STRAND DNA BINDING PROTEIN - A method is disclosed in which circular DNA molecules are amplified preferentially in a mixture of circular DNA molecules and linear DNA molecules by the inclusion of single strand DNA binding protein. | 03-17-2011 |
20110046361 | METHOD FOR SEPARATION OF DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS - The invention provides systems, methods and kits for the separation and/or purification of double-stranded and single-stranded nucleic acids. The method includes first mixing a sample containing the double-stranded nucleic acid and the single-stranded nucleic acid with a pH-neutral, buffered solution consisting essentially of a chaotropic salt and a pH buffer to generate a mixture; then applying the mixture to a first mineral support for the double-stranded nucleic acid to bind; and collecting the flow-through which contains unbound single-stranded nucleic acid. The method further includes adjusting the pH of the flow-through to an acidic pH, and applying the acidified flow-through to a second mineral support for the single-stranded nucleic acid to bind. Alternatively the flow-through can be mixed with a lower aliphatic alcohol prior to loading of the second column. The double-stranded and the single-stranded nucleic acids bound can be eluted from the mineral supports respectively. | 02-24-2011 |
20100331534 | NUCLEIC ACID PURIFICATION METHOD - The invention provides an modified method for the separation of nucleic acids from cells, comprising: generating an aqueous solution containing the nucleic acid by lysing the cells with a lysis solution including SDS and salt; and separating the nucleic acids of interest from other cellular components. The improvement includes adding a non-ionic detergent in the lysis solution such that SDS is not precipitated and no heating of the solution is required prior to cellular lysis. The preferred non-ionic detergents are the polysorbate family of compound, including TWEEN® 20. Also disclosed are composition and kit for performing the modified method. | 12-30-2010 |
20100292446 | ISOLATION OF DNA, RNA AND PROTEIN FROM A SINGLE SAMPLE - The invention provides a method for the separation and purification of two or three cellular components selected from genomic DNA, RNA and proteins from a single biological sample. The method comprises generating an aqueous solution containing the cellular components by lysing cells with a lysis solution; contacting the aqueous solution with an ion exchanger for genomic DNA and RNA to bind to the ion exchanger; collecting the flow-through which contains unbound proteins; eluting RNA from the ion exchanger; and eluting DNA from the ion exchanger. For the purification of any two of the cellular components, one of the components is not collected. The invention also provides reagent kits for carrying out the methods. | 11-18-2010 |
20100246926 | SYSTEM AND METHOD FOR DISTINGUISHING BETWEEN BIOLOGICAL MATERIALS - The invention provides a method for distinguishing biological materials. The method provides: providing at least one segmented image of at least two cells; applying a distance transform to the at least one segmented image of the confluent cells; applying a region growing technique to the distance transform of the at least one segmented image to form a region grown image, wherein a plurality of regions are formed in the at least one segmented image; assigning at least one label to at least one of the plurality of regions of the at least one segmented image of the confluent cells; applying a merging technique to at least two of the plurality of regions if it is determined that at least two of the plurality of regions are neighboring regions; determining whether to assign a same label to the neighboring regions or retain existing labels; and merging the neighboring regions of the region grown image if labels are changed to form at least one image of at least one cell. | 09-30-2010 |
20100238968 | ISOTHERMAL TITRATION MICROCALORIMETER APPARATUS AND METHOD OF USE - An automatic pipette assembly for an isothermal titration micro calorimetry system, comprising a pipette housing, a syringe with a titration needle arranged to be inserted into a sample cell for supplying titrant, and a linear activator for driving a plunger in the syringe, the titration needle is rotatable with respect to the housing and is provided with a stirring paddle arranged to stir fluid in the sample cell, wherein the automatic pipette assembly comprises a stirring motor for driving the rotation of the titration needle. There is also provided an isothermal titration micro calorimetry system. | 09-23-2010 |
20100135853 | AUTOMATIC ISOTHERMAL TITRATION MICROCALORIMETER APPARATUS AND METHOD OF USE - Automated isothermal titration micro calorimetry (ITC) system comprising a micro calorimeter with a sample cell and a reference cell, the sample cell is accessible via a sample cell stem and the reference cell is accessible via a reference cell stem. The system further comprises an automatic pipette assembly comprising a syringe with a titration needle arranged to be inserted into the sample cell for supplying titrant, the pipette assembly comprises an activator for driving a plunger in the syringe, a pipette translation unit supporting the pipette assembly and being arranged to place pipette in position for titration, washing and filling operations, a wash station for the titrant needle, and a cell preparation unit arranged to perform operations for replacing the sample liquid in the sample cell when the pipette is placed in another position than the position for titration. | 06-03-2010 |
20100105109 | MULTIPLY-PRIMED AMPLIFICATION OF CIRCULAR NUCLEIC ACID SEQUENCES - Improved processes for the amplification of target DNA sequences in the form of single or double stranded circular DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed. The product of this amplification is used for analysis by restriction enzyme digestion or DNA sequencing and other analyses that involve hybridization. Kits containing components for use in the method are also described. Also described are further uses of this amplified DNA in sequencing, genotyping and haplotyping, and other molecular biology applications. | 04-29-2010 |
20100090127 | TIME RESOLVED FLUORESCENT IMAGING SYSTEM - This invention provides a system and method that allows for time-resolved fluorescent imaging of fluorescent samples. The user is able to receive temporally filtered pictures of the sample with a reduced amount of the scattered excitation light and the short lived background fluorescence. The system allows for adjustment of fluorescent gating time and delay time. | 04-15-2010 |
20100033812 | SYSTEM AND METHOD FOR ADJUSTING THE SPHERICAL ABERRATION OF OBJECTIVE LENSES - A spherical aberration adjustment system is disclosed, which includes a plurality of objective lenses, where at least one of the plurality of objective lenses has a spherical aberration collar. The plurality of objective lenses are mounted onto an objective holder, where the objective holder is configured to place the at least one of the plurality of objective lenses in an imaging position. A driving mechanism is coupled by a mechanical link to the at least one of the plurality of objective lenses, where the mechanical link is configured to transmit motion from the driving mechanism to the spherical aberration collar. A control system is configured to manipulate the driving mechanism to move the spherical aberration collar of the at least one of the plurality of objective lenses in the imaging position to a specific spherical aberration adjustment setting. | 02-11-2010 |
20100021993 | SYSTEM FOR ASSEMBLING AND UTILIZING SENSORS IN CONTAINERS - A system for measuring parameters in a container is disclosed. A container has a solution. A protective layer is deposited over at least one sensor and at least one wall of the container, where the protective layer is attached to the wall of the container to form a seal between the container and the at least one sensor. The at least one sensor is configured to have an operable electromagnetic field based on a thickness of the container and the protective layer. The at least one sensor in conjunction with a tag is in proximity to an impedance analyzer and a reader that constitute a measurement device. The at least one sensor is configured to determine at least one parameter of the solution. The tag is configured to provide a digital ID associated with the at least one sensor, where the container is in proximity to the reader and an impedance analyzer. The impedance analyzer is configured to receive a given range of frequencies from the at least one sensor based on the measured complex impedance over the given range of frequencies. | 01-28-2010 |
20100015628 | AMBIENT TEMPERATURE STABLE CHEMICAL/BIOLOGICAL REAGENTS ON MEMBRANES OR FILTERS - The present invention provides a biological sample preparation system including ambient temperature stable reagent mixture and a separation filter or membrane. In particular, the system includes a dried reagent in a glassy, porous state, on top of a separation column, whereby sample preparation is streamlined and simplified. Also provided are methods of making and using the system. A kit for preparing a biological sample is also provided. | 01-21-2010 |
20090325263 | PREPARATION OF GLASSIFIED BIOLOGICAL REAGENTS - The invention related to a method of making a dried reagent preparation, comprising the steps of: providing an aqueous solution of at least one buffered biological reagent; mixing a glass forming filler material with the buffered reagent solution to form a mixture wherein the concentration of the filler material is sufficient to facilitate formation of a glassy, porous composition; dispensing the mixture in the form of substantially uniform droplets into wells of a multi-well container, wherein a single droplet is dispensed into each well; drying the droplets in the container to form the reagent preparation. The reagent preparation is water soluble and has a Tg sufficient for room temperature stability. | 12-31-2009 |
20090170167 | SINGLE ENZYME SYSTEM FOR FAST, ULTRA LONG PCR - The present invention provides methods, formulation and kits for the synthesis of long nucleic acid fragments. An improved PCR method is provided for amplifying long DNA fragments. In particular, a single thermostable DNA polymerase is used for the rapid amplification of over 10 kb long DNA fragments. Also provided is a method for extending long complementary DNA strands using this single enzyme formulation. | 07-02-2009 |
20090143570 | METHOD FOR ISOLATION OF GENOMIC DNA, RNA AND PROTEINS FROM A SINGLE SAMPLE - The invention provides systems, methods and kits for the separation and/or purification of at least two cellular components selected from genomic DNA, RNA and proteins. The method includes first lysing a biological sample to generate an aqueous solution containing the cellular components; then applying the aqueous solution to a first mineral support under conditions for genomic DNA to bind; and collecting the flowthrough which contains unbound total RNA and proteins. The method further includes applying the flowthrough to a second mineral support under conditions for RNA to bind, and collecting the flowthrough which contains proteins. The genomic DNA and total RNA bound can be eluted while the protein in the flowthrough can be further purified. Further the total RNA isolated could be used to isolate small RNA such as microRNA. | 06-04-2009 |
20090143234 | QUALITY CONTROL METHODS FOR ARRAYED OLIGONUCLEOTIDES - We disclose quality controls methods that allow quick and accurate verification of a test oligonucleotide deposited on a solid support. It is especially useful for the verification of oligonucleotides representing alleles of a multi-allelic locus. It employs single base extension, with labeled dideoxynucleotides, to locate and verify the identity of test oligonucleotides. This approach involves synthesizing a complement probe oligonucleotide for each oligonucleotide being tested. Probe oligonucleotides are optionally grouped. They are then hybridized to test oligonucleotides, and the hybridized pair is subject to single base extension and detection. It requires the presence of one unique base, either in the last two bases at the free hanging end of the test oligonucleotide—as opposed to the end anchored to the solid support surface, or in the last two bases at one end of the probe oligonucleotide. | 06-04-2009 |
20080287305 | Method for Nucleic Acid Analysis - This invention provides methods for nucleic acid analysis. A closed complex of nucleic acid template, nucleotide and polymerase can be formed during polymerase reaction, absent divalent metal ion. This is used to trap the labeled nucleotide complementary to the next template nucleotide in the closed complex. Detection of the label allows determination of the identity of this next correct nucleotide. Identification can be either in place, as part of the complex, or as the dye is eluted from the complex when the reaction cycle is completed by the addition of divalent metal ion. In this way, sequential nucleotides of a DNA can be identified, effectively determining the DNA sequence. This method can be applied to nucleic acid single molecules or to collections of identical or nearly identical sequence such as PCR products or clones. Multiple templates can be sequenced in parallel, particularly if they are immobilized on a solid support. | 11-20-2008 |
20080264852 | Filtration Cassettes - The present invention relates to filtration cassette, components thereof and methods of making them. A filtration cassette comprises a plurality of subassemblies of which at least one comprises a filter screen encompassed by two filter membranes or one filter membrane and an impermeable film which are joined together around their peripheries. | 10-30-2008 |
20080262741 | METHOD AND APPARATUS FOR SCREENING CHEMICAL COMPOUNDS - Methods and apparatus for screening large numbers of chemical compounds and performing a wide variety of fluorescent assays, including live cell assays. The methods utilize a laser linescan confocal microscope with high speed, high resolution and multi-wavelength capabilities and real time data-processing. Imaging may be done at video-rates and with use of ultraviolet illumination. | 10-23-2008 |