Carnegie Institution of Washington Patent applications |
Patent application number | Title | Published |
20160097088 | Methods of Genome Sequencing and Epigenetic Analysis - Novel methods of ChIP-seq are disclosed herein. These methods of ChIP-seq employ carrier DNA to prevent loss of DNA samples. The greater DNA yields achieved by this invention permit ChIP-seq of a small number of cells, permitting epigenetic analysis of primary cells of limited quantity. | 04-07-2016 |
20150072375 | GENETICALLY ENCODED PROBE FOR QUANTIFICATION OF LACTATE AND METHODS FOR QUANTIFYING METABOLIC RATES AND LACTATE TRANSPORT - A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed. | 03-12-2015 |
20140359899 | Sucrose Transporters and Methods of Generating Pathogen-Resistant Plants - The present invention relates to genetically modified plant cells that have altered expression or activity of at least one sucrose efflux transporter compared to levels of expression or activity of the at least one sucrose efflux transporter in an unmodified plant cell. | 12-04-2014 |
20140323692 | Development of Sensitive FRET Sensors and Methods of Using the Same - Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture. | 10-30-2014 |
20140090111 | Plant Hormone Biosensors - The invention provides fusion proteins comprising at least two fluorescent proteins, with the fluorescent proteins emitting different wavelengths of light from one another, at least one plant hormone binding domain that changes three-dimensional conformation upon specifically binding to a plant hormone, and two linker peptides, with the first linker linking the first fluorescent protein to the N-terminus of the plant hormone binding domain and the second linker linking the second fluorescent protein to the C-terminus of the plant hormone binding domain. The invention also provides for methods of using the fusion proteins of the present invention and nucleic acids encoding the fusion proteins. | 03-27-2014 |
20140051101 | Luciferase Reporter System for Roots and Methods of Using the Same - The present invention relates to a systems and methods of using expression of one or two luminescent proteins in a plant root cell to visualize plant root structure as well as to determine how stressors affect gene expression in plant roots while maintaining the natural soil habitat | 02-20-2014 |
20130230492 | GENETIC INHIBITION BY DOUBLE-STRANDED RNA - A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods. | 09-05-2013 |
20130216103 | Remote Sensing Analysis of Forest Disturbances - The present invention provides systems and methods to automatically analyze Landsat satellite data of forests. The present invention can easily be used to monitor any type of forest disturbance such as from selective logging, agriculture, cattle ranching, natural hazards (fire, wind events, storms), etc. The present invention provides a large-scale, high-resolution, automated remote sensing analysis of such disturbances. | 08-22-2013 |
20120322150 | Mechanism and Method for Regulating Glycogen Synthase Kinase 3 (GSK3)-Related Kinases - The present invention relates to a novel mechanism for regulating GSK3 kinases, including BIN2 and human GSK3-beta, by dephosphorylating GSK3 kinases through the PP1 phosphatase, such as the plant BSU1 phosphatases and human PP1-gamma. | 12-20-2012 |
20120202244 | Antibodies Capable of Specifically Binding to a Specific Amino Acid Sequence - The present invention provides for an antibody or fragment thereof capable of specifically binding to an epitope of the amino acid sequence CDPAFLYKVVD (SEQ ID NO:1) or a fragment of at least 5, 6, or 7 amino acids thereof. | 08-09-2012 |
20110280790 | Production of Large, High Purity Single Crystal CVD Diamond - The invention relates to single crystal diamond with optical quality and methods of making the same. The diamond possesses an intensity ratio of the second-order. Raman peak to the fluorescence background of around 5 or greater. | 11-17-2011 |
20100168455 | Production of Hydroxylated Fatty Acids in Genetically Modified Plants - This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants. In addition, the use of genes encoding fatty acid hydroxylases or desaturases to alter the level of lipid fatty acid unsaturation in transgenic plants is described. | 07-01-2010 |
20100043097 | Brassinosteroid Regulated Kinases (BRKs) That Mediate Brassinosteroid Signal Transduction and Uses Thereof - The present invention identifies a novel family of kinases regulated by brassinosteroids, referred to as BRKs (brassinosteroid regulated kinases) or BSKs (brassinosteroid signaling kinases). The present invention provides methods for modulating the response of a plant cell to a brassinosteroid using BRKs. | 02-18-2010 |
20090311786 | Levels and/or Sustainability of DNA-based Gene Expression - The invention encompasses methods for improving the level and/or sustainability of expression for a target nucleic acid in a eukaryotic cell comprising: (a) modifying the target nucleic acid to introduce or to comprise signals that limit or constrain the positions of nucleosome cores, and (b) introducing the modified target nucleic acid into the eukaryotic cell, wherein the modified target nucleic acid has improved levels and/or sustainability of expression compared to original unmodified nucleic acid. | 12-17-2009 |
20090151025 | Indeterminate Gametophyte 1 (ig1), Mutations of ig1, Orthologs of ig1, and Uses Thereof - This invention provides the genes for indeterminate gametophyte1 (ig1), mutants of ig1, homologs of ig1, and orthologs of ig1, as well as the proteins encoded by these genes. This invention also provides compositions and methods which utilize these genes and proteins. | 06-11-2009 |
20090070902 | Production of hydroxylated fatty acids in genetically modified plants - This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants. In addition, the use of genes encoding fatty acid hydroxylases or desaturases to alter the level of lipid fatty acid unsaturation in transgenic plants is described. | 03-12-2009 |