METHODS AND COMPOSITIONS FOR THE DIAGNOSIS OF SEPSIS USING GAMMA PEPTIDE NUCLEIC ACIDS

Abstract

The present disclosure provides for compositions of .gamma.PNA probes. Additionally, the present disclosure provide for methods and kits using .gamma.PNA probes for the diagnosis of sepsis.

Description

RELATED APPLICATIONS

[0001] This application is the U.S. National Stage of International Application No. PCT/US2013/041628, with an international filing date of May 17, 2013, which claims the benefit of and priority to U.S. Provisional Application No. 61/649,342, filed May 20, 2012 and U.S. Provisional Application No. 61/799,772, filed on Mar. 15, 2013, the contents of which are incorporated by reference herein in their entirety.

FIELD OF THE DISCLOSURE

[0002] This disclosure generally relates to methods, compositions, and kits that use peptide nucleic acid (PNA) as probes for the diagnosis of sepsis.

BACKGROUND

[0003] Sepsis, a spectrum of severe immune disorders triggered by systemic infections, is a leading cause of morbidity and mortality. In the U.S. alone, 751,000 sepsis cases occur annually, leading to 210,000 mortalities and an economic burden of $24B. The primary causes of sepsis are usually symptomatic bacteremia or fungemia, a diagnosis that can only be determined by laboratory testing. Detection of the infecting pathogen is essential to identifying patients and initiating the proper antimicrobial therapy to avert or lessen a sepsis reaction. Traditionally, the first step in this process is a time-intensive step of culturing the unknown pathogen from a patient specimen for a period of 1-5 days. The culturing step results in a delay of effective treatment just at the earliest time of infection, which is a crucial therapeutic window when therapy has the maximum benefit. Each single hour delay in proper treatment increases the probability of mortality by 7.6%.

SUMMARY

[0004] The present disclosure describes methods for diagnosing sepsis that do not require the time-consuming culturing step. In one embodiment, the method comprises first contacting a plurality of .gamma.PNA capture probes to genomic material in a clinical sample obtained from a subject suspected of having sepsis, wherein the .gamma.PNA capture probes comprise at least one sequence from one or more of the groups of probes selected from the group consisting of: SEQ ID NOS: 1-18 (provided in Table 1), SEQ ID NOS: 19-22 (provided in Table 2), SEQ ID NOS: 23-28 (provided in Table 3), SEQ ID NOS: 29-34 (provided in Table 4), SEQ ID NOS: 35-38 (provided in Table 5), SEQ ID NOS: 39-57 (provided in Table 6), SEQ ID NOS: 58-72 (provided in Table 7), SEQ ID NOS: 73-91 (provided in Table 8), SEQ ID NOS: 92-94 (provided in Table 9), SEQ ID NOS: 95-97 (provided in Table 10), SEQ ID NOS: 98-110 (provided in Table 11), SEQ ID NOS: 111-113 (provided in Table 12), SEQ ID NOS: 114-117 (provided in Table 13), SEQ ID NOS: 118-119 (provided in Table 14), SEQ ID NOS: 120-121 (provided in Table 15), SEQ ID NOS: 122-153 (provided in Table 16), SEQ ID NOS: 154-166 (provided in Table 17), SEQ ID NOS: 167-190 (provided in Table 18), SEQ ID NOS: 191-193 (provided in Table 19), SEQ ID NOS: 194-196 (provided in Table 20), SEQ ID NOS: 197-211 (provided in Table 21), SEQ ID NOS: 212-215 (provided in Table 22), SEQ ID NOS: 216-230 (provided in Table 23), complementary sequence thereof, and functional equivalents thereof; followed by the steps of heating the .gamma.PNA capture probes and the sample; invading a plurality of targeted sepsis-related genomic material by the .gamma.PNA capture probes; and detecting a presence of one or more targeted genomic material. Detection of the presence of targeted genomic material indicates the presence of a sepsis infection.

[0005] In some embodiments, the detection of targeted genomic material comprises of adding a plurality of .gamma.PNA reporter probes, which comprise of at least one sequence from one or both of the groups of probes: SEQ ID NOS: 231-248 (provided in Table 24) and SEQ ID NOS: 249-309 (provided in Table 25), complementary sequence thereof, and functional equivalents thereof; heating the .gamma.PNA capture probe, .gamma.PNA reporter probes, and the sample; and invading of the .gamma.PNA reporter probes to the targeted genomic material, wherein the .gamma.PNA reporter probes are used to detect the targeted genomic materials.

[0006] In some embodiments, the contacting step is preceded by an amplification step comprising an enzymatic amplification of the of targeted sepsis-related genomic material.

[0007] In some embodiments, the genomic material in the clinical specimen is sheared.

[0008] In some embodiments, the .gamma.PNA capture probes are bound to a support substrate. In some embodiments, a first carbon-linker, comprising of at least three carbons, binds the .gamma.PNA capture probes to the support substrate. In some embodiments, the support substrate is selected from the group consisting of: a magnetic bead, a bead, a well, a plate, for example polystyrene microtiter plate, a test tube, a stick, for example a dipstick, plastic, glass, and a chip or biochip. In some embodiments, the support substrate is coated with Avidin, Neutravidin, or Streptavidin.

[0009] In some embodiments, the .gamma.PNA capture probes and/or .gamma.PNA reporter probes comprise one or more functional moiety selected from the group consisting of a binding molecule, a spacer group, a linker group, a hydrophobicity-changing group, a charge-inducing group, and a structural change-inducing group. In some embodiments, the spacer group is selected from the group consisting of: (ethylene)glycol, di(ethylene)glycol, tri(ethylene)glycol, poly(ethylene)glycol, 6-carbon linker, and 12 carbon linker. In some embodiments, the linker group is selected from the group consisting of: COOH group, NETS-ester group, malemide chemistry, Click chemistry, streptavidin, and biotinylation. In some embodiments, the hydrophobicity-changing group is selected from the group consisting of: a naturally polar or charged side group or linker that decreases hydrophobicity, and a naturally apolar and uncharged side group or linker that increases hydrophobicity. In some embodiments, the charge-inducing group is selected from the group consisting of: COOH group, NH.sub.3 groups, OH groups, and metallic ions. In some embodiments, the structural change-inducing group induces a chemical modification along the peptide backbone of PNA and is selected from the group consisting of: amino acid-based side chain, nanoparticle, small molecule or intercalating agent. In some embodiments, the .gamma.PNA probe comprises biotin or hapten.

[0010] In some embodiments, detecting the presence of one or more targeted genomic material is through a signal selected from the group consisting of: fluorescence, luminescence, FRET, colorimetric, calorimetric, interference patterns, pH, resistance/conductivity, enzymatic function and kinetics, protein structure, and electrical potential.

[0011] In some embodiments, the .gamma.PNA reporter probes comprise a second carbon-linker. In some embodiments, the second carbon-linker comprises of one or more biotinylation sites.

[0012] An alternative embodiment provides for a composition for diagnosing sepsis, wherein the composition comprises a .gamma.PNA probe composition comprising at least one sequence from one or more of the groups of probes selected from the group consisting of: SEQ ID NOS: 1-18, SEQ ID NOS: 19-22, SEQ ID NOS: 23-28, SEQ ID NOS: 29-34, SEQ ID NOS: 35-38, SEQ ID NOS: 39-57, SEQ ID NOS: 58-72, SEQ ID NOS: 73-91, SEQ ID NOS: 92-94, SEQ ID NOS: 95-97, SEQ ID NOS: 98-110, SEQ ID NOS: 111-113, SEQ ID NOS: 114-117, SEQ ID NOS: 118-119, SEQ ID NOS: 120-121, SEQ ID NOS: 122-153, SEQ ID NOS: 154-166, SEQ ID NOS: 167-190, SEQ ID NOS: 191-193, SEQ ID NOS: 194-196, SEQ ID NOS: 197-211, SEQ ID NOS: 212-215, SEQ ID NOS: 216-230, SEQ ID NOS: 231-248, SEQ ID NOS: 249-309, complementary sequence thereof, and functional equivalents thereof.

[0013] In some embodiments, the .gamma.PNA probe comprises a support substrate. In some embodiments, the support substrate is selected from the group consisting of: a magnetic bead, a bead, a well, a plate, for example polystyrene microtiter plate, a test tube, a stick, for example a dipstick, plastic, glass, and a chip or biochip. In some embodiments, the support substrate is coated with Avidin, Neutravidin, or Streptavidin.

[0014] In some embodiments, the .gamma.PNA probe comprises one or more functional moiety selected from the group consisting of a binding molecule, a spacer group, a linker group, a hydrophobicity-changing group, a charge-inducing group, and a structural change-inducing group.

[0015] In some embodiments, the .gamma.PNA probe emits a detectable signal selected from the group consisting of: fluorescence, luminescence, FRET, colorimetric, calorimetric, interference patterns, pH, resistance/conductivity, enzymatic function and kinetics, protein structure, and electrical potential.

[0016] In some embodiments, the .gamma.PNA probe comprises a carbon-linker comprising at least three carbons. In some embodiments, the carbon-linker comprises of one or more biotinylation sites.

[0017] An alternative embodiment provides for a kit for detecting sepsis comprising a .gamma.PNA capture probe composition comprising at least one sequence from one or more of the groups of probes selected from the group consisting of: SEQ ID NOS: 1-18, SEQ ID NOS: 19-22, SEQ ID NOS: 23-28, SEQ ID NOS: 29-34, SEQ ID NOS: 35-38, SEQ ID NOS: 39-57, SEQ ID NOS: 58-72, SEQ ID NOS: 73-91, SEQ ID NOS: 92-94, SEQ ID NOS: 95-97, SEQ ID NOS: 98-110, SEQ ID NOS: 111-113, SEQ ID NOS: 114-117, SEQ ID NOS: 118-119, SEQ ID NOS: 120-121, SEQ ID NOS: 122-153, SEQ ID NOS: 154-166, SEQ ID NOS: 167-190, SEQ ID NOS: 191-193, SEQ ID NOS: 194-196, SEQ ID NOS: 197-211, SEQ ID NOS: 212-215, SEQ ID NOS: 216-230, complementary sequence thereof, and functional equivalents thereof.

[0018] In some embodiments, the kit comprises a .gamma.PNA reporter probe composition comprising at least one sequence from one or both of the groups of reporter probes: SEQ ID NOS: 231-248 and SEQ ID NOS: 249-309, complementary sequence thereof, and functional equivalents thereof.

[0019] In some embodiments, the .gamma.PNA capture probes are bound to a support substrate. In some embodiments, the support substrate is selected from the group consisting of: a magnetic bead, a bead, a well, a plate, for example polystyrene microtiter plate, a test tube, a stick, for example a dipstick, plastic, glass, and a chip or biochip. In some embodiments, the support substrate is coated with Avidin, Neutravidin, or Streptavidin.

[0020] In some embodiments, the .gamma.PNA probe composition emits a detectable signal selected from the group consisting of: fluorescence, luminescence, FRET, colorimetric, calorimetric, interference patterns, pH, resistance/conductivity, enzymatic function and kinetics, protein structure, and electrical potential.

[0021] Another alternative embodiment provides for a method for diagnosing sepsis comprising contacting a plurality of .gamma.PNA reporter probes to genomic material in a clinical sample obtained from a subject suspected of having sepsis, wherein the .gamma.PNA reporter probes comprise at least one sequence from one or both of the groups of reporter probes: SEQ ID NOS: 231-248 and SEQ ID NOS: 249-309, complementary sequence thereof, and functional equivalents thereof; heating the .gamma.PNA reporter probes and the sample; invading a plurality of targeted sepsis-related genomic material by the .gamma.PNA reporter probes; contacting the plurality of sepsis-related genomic material with .gamma.PNA capture probes, wherein the .gamma.PNA capture probes comprise at least one sequence from one or more of the groups of probes selected from the group consisting of: SEQ ID NOS: 1-18, SEQ ID NOS: 19-22, SEQ ID NOS: 23-28, SEQ ID NOS: 29-34, SEQ ID NOS: 35-38, SEQ ID NOS: 39-57, SEQ ID NOS: 58-72, SEQ ID NOS: 73-91, SEQ ID NOS: 92-94, SEQ ID NOS: 95-97, SEQ ID NOS: 98-110, SEQ ID NOS: 111-113, SEQ ID NOS: 114-117, SEQ ID NOS: 118-119, SEQ ID NOS: 120-121, SEQ ID NOS: 122-153, SEQ ID NOS: 154-166, SEQ ID NOS: 167-190, SEQ ID NOS: 191-193, SEQ ID NOS: 194-196, SEQ ID NOS: 197-211, SEQ ID NOS: 212-215, SEQ ID NOS: 216-230, complementary sequence thereof, and functional equivalents thereof; heating the .gamma.PNA reporter probes, the .gamma.PNA capture probes, and the sample; invading the plurality of targeted sepsis-related genomic material by the .gamma.PNA capture probes; and detecting a presence of one or more targeted genomic material, wherein detection of the presence of target genomic material is indicative of sepsis infection.

[0022] In some embodiments, the support substrate is selected from the group consisting of: a magnetic bead, a bead, a well, a plate, for example polystyrene microtiter plate, a test tube, a stick, for example a dipstick, plastic, glass, and a chip or biochip. In some embodiments, the support substrate is coated with Avidin, Neutravidin, or Streptavidin.

[0023] In some embodiments, wherein the .gamma.PNA capture probes and .gamma.PNA reporter probes comprise one or more functional moiety selected from the group consisting of: a binding molecule, a spacer group, a linker group, a hydrophobicity-changing group, a charge-inducing group, and a structural change-inducing group.

[0024] In some embodiments, the .gamma.PNA capture probes and .gamma.PNA reporter probes comprise biotin or hapten.

[0025] In some embodiments, the .gamma.PNA reporter probes emit a detectable signal selected from the group consisting of: fluorescence, luminescence, FRET, colorimetric, calorimetric, interference patterns, pH, resistance/conductivity, enzymatic function and kinetics, protein structure, and electrical potential.

BRIEF DESCRIPTION OF THE DRAWINGS

[0026] FIG. 1A is a schematic of .gamma.PNA capture probes binding to a magnetic bead that is coated with neutravidin.

[0027] FIG. 1B is a schematic of target genomic materials binding to .gamma.PNA capture probes.

[0028] FIG. 1C is a schematic of .gamma.PNA reporter probes binding to immobilized target genomic materials.

[0029] FIG. 1D is a schematic of reporter enzyme conjugates binding to .gamma.PNA reporter probes.

[0030] FIG. 1E is a schematic of adding enzyme substrate to induce a signal.

[0031] FIG. 2 is a gel shift assay demonstrating the specificity of .gamma.PNA probes.

[0032] FIG. 3 is a gel shift assay demonstrating the specificity of .gamma.PNA capture probes for different species of the same genus.

DETAILED DESCRIPTION

[0033] It is to be appreciated that certain aspects, modes, embodiments, variations and features of the invention are described below in various levels of detail in order to provide a substantial understanding of the present invention. The definitions of certain terms as used in this specification are provided below. Unless defined otherwise, all technical and scientific terms used herein generally have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. As used herein the following terms have the following meanings.

Definitions

[0034] As used herein, the term "comprising" or "comprises" is intended to mean that the compositions and methods include the recited elements, but not excluding others. "Consisting essentially of" when used to define compositions and methods, shall mean excluding other elements of any essential significance to the combination for the stated purpose. Thus, a composition consisting essentially of the elements as defined herein would not exclude other materials or steps that do not materially affect the basic and novel characteristic(s) of the claimed invention. "Consisting of" shall mean excluding more than trace elements of other ingredients and substantial method steps. Embodiments defined by each of these transition terms are within the scope as described herein.

[0035] The term "about" when used before a numerical designation, e.g., temperature, time, amount, and concentration, including range, indicates approximations which may vary by (+) or (-) 10%, 5%, or 1%.

[0036] As used in this specification and the appended claims, the singular forms "a", "an" and "the" include plural referents unless the content clearly dictates otherwise. For example, reference to "a cell" includes a combination of two or more cells, and the like.

[0037] As used herein, "sample" refers to blood samples, culture samples, or DNA samples that originate from or are generated from a person or patient that is suspected of having sepsis. The samples taken or derived from the person or patient are believed to contain pathogenic genomic material related to sepsis. Additionally, sample can refer to mixture of genomic material that is to be tested for the presence sepsis related pathogenic materials.

[0038] As used herein, "pathogenic or bacterial genomic material" or "targeted sepsis-related genomic material" or "targeted genomic material" refer to DNA, RNA, oligonucleotides, or polynucleic acids related to any genus or species of pathogens related to sepsis. Additionally, DNA and RNA are broadly used to include, but not limited to, ribosomal DNA and RNA (rDNA and rRNA), messenger RNA (mRNA), transfer DNA (tDNA), and mitochondrial DNA (mDNA). Examples of such pathogens include, but are not limited to, Staphylococcus epidermidis, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, and Escherichia coli.

[0039] As used herein, "support substrate" refers a solid substrate upon which .gamma.PNAs can be immobilized. In one embodiment, the support substrate is a magnetic bead, a bead, a well, a plate, for example polystyrene microtiter plate, a test tube, a stick, for example a dipstick, plastic, glass, and a chip or biochip. In some embodiments, the support substrate is silicon based or coated with either a semiconductive, conductive, or insulating material. In some embodiments, the support substrate includes metallic surfaces that are functionalized. In other embodiments the solid substrate may be manufactured from polymers, nylon, nitrocellulose, polyacrylamide, oxides. In some embodiments, the solid support is manufactured from multiple materials. In some embodiments, the surface of the support substrate is coated with an aminosilane or any other commonly known surface treatments, such as epoxysilanes.

[0040] As used herein, "immobilizing" describes binding of .gamma.PNA capture probes onto a solid, support substrate prior to the introduction of a sample. Immobilization of the .gamma.PNA capture probes sequences on a support substrate can be achieved through various means such as covalent binding protocols or non-covalent binding protocols. Binding modalities and chemistries are commonly known to those skilled in the art.

[0041] As used herein, "signal" refers to that which is detectable though optical modalities, or through electrical modalities, or through biological modalities, or through chemical modalities.

[0042] As used herein, "diagnosis" refers to determining or identifying the presence or absence of one or more sepsis-inducing pathogen in a patient. Additionally, diagnosis can also refer to determining a patient's susceptibility to sepsis.

[0043] As used herein, "washing" refers to steps to remove unwanted, unbound, weakly bound, non-specifically bound genomic and other non-genomic material from the vicinity of the PNA probe. Washing steps are well established within the field and have been optimized for numerous biological assays such as ELISA, Western blot assays, Southern blot assays, Northern Blot assays, DNA microarrays, RNA microarrays, protein microarrays, and LiPA. Washing steps, and optimizing of the washing steps are well established and known to those skilled in the art.

[0044] The term "invasion" or "invade" refers to .gamma.PNA probes, both capture and reporter, binding to target sequences using either natural or induced structural fluctuations, referred to as DNA breathing or DNA bubbles. Nucleic acids may, on occasion, present their nucleobases to the bulk, meaning the nucleobases are not hidden within the structure. When this occurs, the .gamma.PNA, may bind to those exposed nucleobases through typical Watson Crick base-pairing rules. Upon the closure of this DNA bubble, the .gamma.PNA remains bound to the nucleic acid region effectively displacing, locally, the complementary nucleic acid strand--hence .gamma.PNA invades the nucleic acid structure locally.

[0045] Culture-free diagnostic tools are required for the timely and proper treatment of microbial pathogens which induce a septic response in afflicted patients. While recent advances in molecular diagnostics have revolutionized numerous disease areas in clinical testing, severe technical limitations prevent molecular techniques from having significant impact in cases of bacteremia or fungemia. Identification of the infecting pathogen and its susceptibility to antimicrobial therapy still require a time-intensive step of culturing. Thus, the realities of sepsis remain grim; a mortality rate of 28% with 210,000 annual deaths in the U.S. alone. The present disclosure describes compounds, methods, and kits related to a culture-free approach to sepsis pathogen identification and susceptibility analysis.

Peptide Nucleic Acid

[0046] Peptide nucleic acids (PNAs) are synthetic, or non-naturally, occurring oligomers, which have displayed the ability to bind to both DNA and RNA according to either Watson-Crick and/or Hoogstgein base pairing. In PNA, the negatively charged sugar-phosphate backbone of natural DNA or RNA has been replaced with a neutral peptide backbone. PNA's neutral backbone negates the energy penalty natural probes must expend to overcome the mutual repulsion of their negatively charged phosphate backbone. Thus, PNA binds to nucleic acids (NAs) with much greater affinities than natural probes. Other advantages of PNAs in general include: the ability to bind to both natural and synthetic targets, fast binding kinetics, and the ability to add chemical moieties such as, but not limited to, fluorescent dyes, biotin, protein binding agents, radio-labeling, or quantum dots.

[0047] A new class of PNA, termed .gamma.PNA, is PNA with a simple backbone modification at the .gamma.-position of the N-(2-aminoethyl) glycine backbone that generates a chiral center. In an unbound state, the configuration of ordinary PNA or DNA probes is a random, globular structure. In contrast, unbound .gamma.PNA probes assume a right-handed helix structure, pre-organized for Watson-Crick base pairing, which greatly facilitating binding.

[0048] .gamma.PNA probes have several major advantages of over natural DNA probes and ordinary PNA probes. Some of the advantages include:

[0049] 1) .gamma.PNA has substantially greater affinity to nucleic acids than other natural or synthetic probes. Typical Tm values for .gamma.PNA/DNA 15 bp hybrids are .about.20.degree. C. higher (Tm>95.degree. C.) than equivalent ordinary PNA/DNA hybrids and .about.50.degree. C. higher than natural dsDNA. .gamma.PNA Kd values are even comparable to antibodies (nM-pM).

[0050] 2) .gamma.PNA is significantly more sequence specific. Ordinary PNAs are commonly used as primer clamps to overcome the poor sequence specificity of natural DNA by removing the likelihood of PCR beacons binding to slightly mismatched regions. .gamma.PNAs have even greater sequence specificity than ordinary PNAs, as reflected in the greater increase in .DELTA.Tm when a mismatch is induced.

[0051] 3) .gamma.PNA has the unique ability to invade structured nucleic acids, such as double stranded DNA (dsDNA) and RNA, which facilitates a less complex method for target identification. .gamma.PNA's affinity to single-stranded NAs is so high that it has the ability to naturally invade the double stranded structure and bind through standard Watson-Crick base pairing to the correct sequence. Binding to single-stranded NAs occurs just as it would for other synthetic and natural nucleic acid probes.

PNA Targets and Sequences

[0052] Comparative analysis of ribosomal DNA (rDNA) sequences has become a well-established method for establishing phylogenetic relationships between microbial species. Microbial rDNA are among the most highly conserved and most rigorously studied regions in a microbial genome. Minute differences in rDNA sequences enable the design of highly specific probes for target regions, which are specific to one or more pathogens.

[0053] Polymicrobial infections are problematic in that numerous pathogens with their own inherent resistance traits induce similar pathophysiological traits in the host during sepsis. Despite the similarity in host response and the high similarity at the genomic level of numerous pathogens; treatment regimens could vary significantly depending on species traits as well as the presence or absence of genes which encode for antimicrobial resistance.

[0054] Sequence analysis for specific genes that might encode for a resistance to a particular antimicrobial compound has likewise been established. Multiple regions in these genes are highly conserved and can be used to create markers for targeting. The targeting the highly conserved regions would enable the detection of a gene sequence that encodes for antimicrobial resistance.

[0055] The .gamma.PNA probes preferably target rDNA/rRNA sequence of the microbial pathogen. In the present disclosure, the .gamma.PNA probe sequences all relate to identifying bacterium involved in sepsis. Tables 1-25 describes a non-exclusive list of sequences capable of identifying bacterium involved with sepsis. Since the .gamma.PNA probes will bind to dsDNA, one skilled in the art would know that the reverse-complementary sequences of the sequences in Tables 1-25 can also be .gamma.PNA probe sequences. Thus, in some embodiments, the target sequence may be the reverse-complementary sequence to those identified here.

[0056] In some embodiments, .gamma.PNA probe sequences are those which will bind to the corresponding rDNA/rRNA target sequences through Watson-Crick base-pairing. Additional base-pairing methods such as Hoogstein have been demonstrated with other PNA variants (such as bis-PNA).

[0057] Since PNAs do not have phosphate-sugar backbone, orientation is guided by the terminus of the peptide backbone for proper binding. Therefore, the C-terminus aligns with the 5' end of the DNA/RNA target, and the N-terminus aligns with the 3' end of the DNA/RNA target.

[0058] In addition to the natural nucleobases, the inclusion of modified or synthetic nucleobases may also be included to enhance .gamma.PNA characteristics. A common synthetic nucleobase for use with .gamma.PNA is typically called the `G-clamp` which refers to a pseudo-cytosine (9-(2-guanidinoethoxy) phenoxazine). Another common synthetic nucleobase used in PNAs is the J-base which carries a hydrogen atom at the N3 position allowing its Hoogsteen pairing with a guanine base without protonation.

TABLE-US-00001 TABLE 1 .gamma.PNA capture probe sequences for Staphylococcus aureus Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 1 CGG AAC ATC TTC TTC SEQ ID NO: 2 TCA GAA GAT GCG GAA SEQ ID NO: 3 CCT GAT AAG CGT GAG SEQ ID NO: 4 GAG TCC ACT TAG GCC SEQ ID NO: 5 CAC TTA GGC CCA CCA SEQ ID NO: 6 AGG CCC ACC ATT ATT SEQ ID NO: 7 AAC GGA CGA GAA GCT SEQ ID NO: 8 TCC TTT GAC AAC TCT SEQ ID NO: 9 AAC GGA CGA GAA GCT SEQ ID NO: 10 AGA GAT AGA GCC TTC SEQ ID NO: 11 TTT GAC AAC TCT AGA SEQ ID NO: 12 CTT CTC TGA TGT TAG SEQ ID NO: 13 GGA TAA TAT TTT GAA SEQ ID NO: 14 TTC AAA AGT GAA AGA SEQ ID NO: 15 AGA CGG TCT TGC TGT SEQ ID NO: 16 ATC CGC GCT GCA TTA SEQ ID NO: 17 AGA ACA TAT GTG TAA SEQ ID NO: 18 TAA CCT TTT AGG AGC

TABLE-US-00002 TABLE 2 .gamma.PNA capture probe sequences for Enterococcus faecalis Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 19 ACA AGG ACG TTA GTA SEQ ID NO: 20 CTT TCC TCC CGA GTG SEQ ID NO: 21 CCT ACC CAT CAG AGG SEQ ID NO: 22 GGA CGT TAG TAA CTG

TABLE-US-00003 TABLE 3 .gamma.PNA capture probe sequences for Enterococcus faecium Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 23 CTT GCT CCA CCG GAA SEQ ID NO: 24 CTT TTT CCA CCG GAG SEQ ID NO: 25 ATG GTT TTG ATT TGA SEQ ID NO: 26 CTT TTT CCA CCG GAG SEQ ID NO: 27 CGT ATA ACA ATC GAA SEQ ID NO: 28 CGT ATA ACA ATC AAA

TABLE-US-00004 TABLE 4 .gamma.PNA capture probe sequences for Escherichia coli Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 29 AAC AGG AAG AAG CTT SEQ ID NO: 30 GGA GTA AAG TTA ATA SEQ ID NO: 31 ATA CCT TTG CTC ATT SEQ ID NO: 32 CAT CTG ATA CTG GCA SEQ ID NO: 33 TTG CTT CTT TGC TGA SEQ ID NO: 34 AGC TTG AGT CTC GTA

TABLE-US-00005 TABLE 5 .gamma.PNA capture probe sequences for Staphylococcus epidermidis Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 35 GCT CCT CTG ACG TTA SEQ ID NO: 36 ATA ATA TAT TGA ACC SEQ ID NO: 37 TTC AAT AGT GAA AGA SEQ ID NO: 38 AAC TAT GCA CGT CTT

TABLE-US-00006 TABLE 6 .gamma.PNA capture probe sequences for Pseudomonas aeruginosa Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 39 GAG CGG ATG AAG GGA SEQ ID NO: 40 CTT GCT CCT GGA TTC SEQ ID NO: 41 AAT CTG CCT GGT AGT SEQ ID NO: 42 ATA ACG TCC GGA AAC SEQ ID NO: 43 CCG CAT ACG TCC TGA SEQ ID NO: 44 AGA TGA GCC TAG GTC SEQ ID NO: 45 GAC GAT CCG TAA CTG SEQ ID NO: 46 CAG TAA GTT AAT ACC SEQ ID NO: 47 CAA CAG AAT AAG CAC SEQ ID NO: 48 TCC AAA ACT ACT GAG SEQ ID NO: 49 CTG AGC TAG AGT ACG SEQ ID NO: 50 AAT TTC CTG TGT AGC SEQ ID NO: 51 GCG TAG ATA TAG GAA SEQ ID NO: 52 ACC ACC TGG ACT GAT SEQ ID NO: 53 TGT CGA CTA GCC GTT SEQ ID NO: 54 AGC CGT TGG GAT CCT SEQ ID NO: 55 TGA GAT CTT AGT GGC SEQ ID NO: 56 AAC TCA GAC ACA GGT SEQ ID NO: 57 TTG TCC TTA GTT ACC

TABLE-US-00007 TABLE 7 .gamma.PNA capture probe sequences for Streptococcus pneumoniae Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 58 CTG AAG GAG GAG CTT SEQ ID NO: 59 AGG AGC TTG CTT CTC SEQ ID NO: 60 ATG ACA TTT GCT TAA SEQ ID NO: 61 ACT TGC ATC ACT ACC SEQ ID NO: 62 AAT GGA CGG AAG TCT SEQ ID NO: 63 AAG AAC GAG TGT GAG SEQ ID NO: 64 AAA GTT CAC ACT GTG SEQ ID NO: 65 TAT CTT ACC AGA AAG SEQ ID NO: 66 TTA GAT AAG TCT GAA SEQ ID NO: 67 AAA GGC TGT GGC TTA SEQ ID NO: 68 TTA ACC ATA GTA GGC SEQ ID NO: 69 AAA CTG TTT AAC TTG SEQ ID NO: 70 ACT TGA GTG CAA GAG SEQ ID NO: 71 TCT CTG GCT TGT AAC SEQ ID NO: 72 CCT CTG ACC GCT CTA

TABLE-US-00008 TABLE 8 .gamma.PNA capture probe sequences for Streptococcus pyogenes Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 73 AGA ACT GGT GCT TGC SEQ ID NO: 74 CTG GTG CTT GCA CCG SEQ ID NO: 75 TTG CAC CGG TTC AAG SEQ ID NO: 76 TAA CCT ACC TCA TAG SEQ ID NO: 77 ATA AGA GAG ACT AAC SEQ ID NO: 78 AGA CTA ACG CAT GTT SEQ ID NO: 79 AGT AAT TTA AAA GGG SEQ ID NO: 80 AAT TGC TCC ACT ATG SEQ ID NO: 81 CTC CAC TAT GAG ATG SEQ ID NO: 82 TTA GAG AAG AAT GAT SEQ ID NO: 83 GAA AAT CCA CCA AGT SEQ ID NO: 84 TGA CGG TAA CTA ACC SEQ ID NO: 85 AAA GGC ATT GGC TCA SEQ ID NO: 86 CTC AAC CAA TGT ACG SEQ ID NO: 87 AAA CTG GAG AAC TTG SEQ ID NO: 88 CTT GAG TGC AGA AGG SEQ ID NO: 89 GCT TAG TGC CGG AGC SEQ ID NO: 90 ATA GAG TTT TAC TTC SEQ ID NO: 91 GGT ACA TCG GTG ACA

TABLE-US-00009 TABLE 9 .gamma.PNA capture probe sequences for Klebsiella pneumonia Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 92 AAG GCG ATA AGG TTA SEQ ID NO: 93 TGC CAG CGG TTA GGC SEQ ID NO: 94 AAG GCG ATG AGG TTA

TABLE-US-00010 TABLE 10 .gamma.PNA capture probe sequences for Enterobacter species Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 95 AAG GCG TTA AGG TTA SEQ ID NO: 96 ATA ACC TTG GCG ATT SEQ ID NO: 97 TGC CAG CGG TCC GGC

TABLE-US-00011 TABLE 11 .gamma.PNA capture probe sequences for Proteus mirabilis Sequence (N-terminus SEQ ID to C-terminus) SEQ ID NO: 98 AAC AGG AGA AAG CTT SEQ ID NO: 99 TTT CTT GCT GAC GAG SEQ ID NO: 100 GGA TCT GCC CGA TAG SEQ ID NO: 101 ATA ATG TCT ACG GAC SEQ ID NO: 102 TAC GGA CCA AAG CAG SEQ ID NO: 103 TTG CAC TAT CGG ATG SEQ ID NO: 104 CGG ATG AAC CCA TAT SEQ ID NO: 105 AAT ACC CTT GTC AAT SEQ ID NO: 106 TCA ATT AAG TCA GAT SEQ ID NO: 107 ATC TGA AAC TGG TTG SEQ ID NO: 108 ATT TAG AGG TTG TGG SEQ ID NO: 109 TTG TGG TCT TGA ACC SEQ ID NO: 110 AGC GAA TCC TTT AGA

TABLE-US-00012 TABLE 12 .gamma.PNA capture probe sequences for Staphylococcus lugdunensis SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 111 GAC TGG GAC AAC TTC SEQ ID NO: 112 ATA ATA TGT TGA ACC SEQ ID NO: 113 GTC TTA GGA TCG TAA

TABLE-US-00013 TABLE 13 .gamma.PNA capture probe sequences for Staphylococcus warneri SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 114 GGA TAA CAT ATT GAA SEQ ID NO: 115 AAA GGC GGC TTT GCT SEQ ID NO: 116 TCT GTT ATC AGG GAA SEQ ID NO: 117 GTA CCT GAT CAG AAA

TABLE-US-00014 TABLE 14 .gamma.PNA capture probe sequences for Staphylococcus hominis SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 118 AGA TGG CTT TGC TAT SEQ ID NO: 119 GAG ATA GAA GTT TCC

TABLE-US-00015 TABLE 15 .gamma.PNA capture probe sequences for Serratia Marcescens SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 120 AAG GTG GTG AGC TTA SEQ ID NO: 121 TTA ATA CGT TCA TCA

TABLE-US-00016 TABLE 16 .gamma.PNA capture probe sequences for Acinetobacter baumannii SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 122 AAG GTA GCT TGC TAC SEQ ID NO: 123 TTG CTA CCG GAC CTA SEQ ID NO: 124 AAT GCT TAG GAA TCT SEQ ID NO: 125 AAT CTG CCT ATT AGT SEQ ID NO: 126 ACA ACA TCT CGA AAG SEQ ID NO: 127 AAA GGG ATG CTA ATA SEQ ID NO: 128 ACC TTG CGC TAA TAG SEQ ID NO: 129 ATG AGC CTA AGT CGG SEQ ID NO: 130 CGA TCT GTA GCG GGT SEQ ID NO: 131 AAC CCT GAT CCA GCC SEQ ID NO: 132 AGG CTA CTT TAG TTA SEQ ID NO: 133 TTT AGT TAA TAC CTA SEQ ID NO: 134 TAC CTA GAG ATA GTG SEQ ID NO: 135 ATA GTG GAC GTT ACT SEQ ID NO: 136 CAG CCA TCT GGC CTA SEQ ID NO: 137 GCC TAA TAC TGA CGC SEQ ID NO: 138 TCT ACT AGC CGT TGG SEQ ID NO: 139 CCT TTG AGG CTT TAG SEQ ID NO: 140 CGA TAA GTA GAC CGC SEQ ID NO: 141 GTC GCA AGA CTA AAA SEQ ID NO: 142 TGG CCT TGA CAT ACT SEQ ID NO: 143 ATA CTA GAA ACT TTC SEQ ID NO: 144 AAT CTA GAT ACA GGT SEQ ID NO: 145 TTT TCC TTA CTT GCC SEQ ID NO: 146 CCA GCA TTT CGG ATG SEQ ID NO: 147 ACT TTA AGG ATA CTG SEQ ID NO: 148 TTG CTA CAC AGC GAT SEQ ID NO: 149 ATG TGA TGC TAA TCT SEQ ID NO: 150 TAA TCT CAA AAA GCC SEQ ID NO: 151 AAG CCG ATC GTA GTC SEQ ID NO: 152 AAT GCC GCG GTG AAT SEQ ID NO: 153 TAG CCT AAC TGC AAA

TABLE-US-00017 TABLE 17 .gamma.PNA capture probe sequences for Stenotrophomonas maltophilia SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 154 AAC GGC AGC ACA GTA SEQ ID NO: 155 TAA GAG CTT GCT CTT SEQ ID NO: 156 GAA TAC ATC GGA ATC SEQ ID NO: 157 AAA CTT ACG CTA ATA SEQ ID NO: 158 ATC CAG CTG GTT AAT SEQ ID NO: 159 GTA CCC AAA GAA TAA SEQ ID NO: 160 TTG TTT AAG TCT GTT SEQ ID NO: 161 AGC TAC CTG GAC CAA SEQ ID NO: 162 TGC AAT TTG GCA CGC SEQ ID NO: 163 AAC GCG TTA AGT TCG SEQ ID NO: 164 CTG CAA GCC GGC GAC SEQ ID NO: 165 AGA AAC CCT ATC TCA SEQ ID NO: 166 AGC ATT GCT GCG GTG

TABLE-US-00018 TABLE 18 .gamma.PNA capture probe sequences for Capture Coagulase-negative staphylococci (CoNS). Sequence (N-terminus Pool # SEQ ID to C-terminus) 1 SEQ ID NO: 167 AAC AGA TAA GGA GCT SEQ ID NO: 168 AAC AGA CGA GGA GCT SEQ ID NO: 169 AAC AGA CAA GGA GCT 2 SEQ ID NO: 170 CTC CTT TGA CGT TAG SEQ ID NO: 171 CTC CTC TGA CGT TAG SEQ ID NO: 172 CTC TTT TGA CGT TAG SEQ ID NO: 173 CTT CTC TGA CGT TAG 3 SEQ ID NO: 174 GGA TAA TAT TTC GAA SEQ ID NO: 175 GGA TAA CAT ATT GAA SEQ ID NO: 176 GGA TAA TAT ATT GAA SEQ ID NO: 177 GGA TAA TAT GTT GAA 4 SEQ ID NO: 178 AGA TGG TTT TGC TAT SEQ ID NO: 179 AGG CGG CTT TGC TGT SEQ ID NO: 180 AGA CGG TTT TGC TGT SEQ ID NO: 181 AGA TGG CTT TGC TAT 5 SEQ ID NO: 182 ACC CGC GCC GTA TTA SEQ ID NO: 183 ACC TGC GCC GTA TTA SEQ ID NO: 184 ATC CGC GCC GTA TTA SEQ ID NO: 185 ATC CGC GCC GCA TTA 6 SEQ ID NO: 186 AGA ACA TAC GTG TAA SEQ ID NO: 187 AGA ACA AAC GTG TAA SEQ ID NO: 188 AGA ACA AAT GTG TAA 7 SEQ ID NO: 189 TAA CCA TTT GGA GCT SEQ ID NO: 190 TAA CCA TTT ATG GAG

TABLE-US-00019 TABLE 19 .gamma.PNA capture probe sequences for Candida tropicalis SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 191 AAT GTC TTC GGA CTC SEQ ID NO: 192 CAT CTT TCT GAT GCG SEQ ID NO: 193 GGC TAG CCT TTT GGC

TABLE-US-00020 TABLE 20 .gamma.PNA capture probe sequences for Candida parapsilosis SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 194 ATC TTT TTT GAT GCG SEQ ID NO: 195 TGG CTA GCC TTT TTG SEQ ID NO: 196 TAT TCA GTA GTC AGA

TABLE-US-00021 TABLE 21 .gamma.PNA capture probe sequences for Candida glabrata SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 197 CTT TAC TAC ATG GTA SEQ ID NO: 198 ATG GTA TAA CTG TGG SEQ ID NO: 199 ATG CTT AAA ATC TCG SEQ ID NO: 200 TCC GAT TTT TTC GTG SEQ ID NO: 201 TGT ACT GGA ATG CAC SEQ ID NO: 202 AAC CCC AAG TCC TTG SEQ ID NO: 203 TTG TGG CTT GGC GGC SEQ ID NO: 204 ACG TTT GGT TCT ATT SEQ ID NO: 205 TAT TCA ATT GTC AGA SEQ ID NO: 206 TGT TTT TTT AGT GAC SEQ ID NO: 207 TAA ATA GTG GTG CTA SEQ ID NO: 208 ATT TGC TGG TTG TCC SEQ ID NO: 209 TAT CGG TTT CAA GCC SEQ ID NO: 210 AGC GAG TCT AAC CTT SEQ ID NO: 211 TCT TGG TAA TCT TGT

TABLE-US-00022 TABLE 22 .gamma.PNA capture probe sequences for Candida albicans SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 212 TTC TGG GTA GCC ATT SEQ ID NO: 213 GCC ATT TAT GGC GAA SEQ ID NO: 214 GGT AGC CAT TTA TGG SEQ ID NO: 215 CTA TCG ACT TCA AGT

TABLE-US-00023 TABLE 23 .gamma.PNA capture probe sequences for Aspergillus species SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 216 TAC CTT ACT ACA TGG SEQ ID NO: 217 ACT ACA TGG ATA CCT SEQ ID NO: 218 TGC TAA AAA CCT CGA SEQ ID NO: 219 TAA AAA ACC AAT GCC SEQ ID NO: 220 TAA CGA ATC GCA TGG SEQ ID NO: 221 TAC CAT GGT GGC AAC SEQ ID NO: 222 AAT CTA AAT CCC TTA SEQ ID NO: 223 AGT ACT GGT CCG GCT SEQ ID NO: 224 GAA CCT CAT GGC CTT SEQ ID NO: 225 GCC TTC ACT GGC TGT SEQ ID NO: 226 TTT CTA TGA TGA CCC SEQ ID NO: 227 TCG GCC CTT AAA TAG SEQ ID NO: 228 GAG TAC ATC ACC TTG SEQ ID NO: 229 CTT GTT AAA CCC TGT SEQ ID NO: 230 AGC TCG TGC CGA TTA

TABLE-US-00024 TABLE 24 .gamma.PNA reporter probe sequences for bacteria SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 231 GGA CTA CCA GGG TAT SEQ ID NO: 232 ACC AGG GTA TCT AAT SEQ ID NO: 233 CGG GAA CGT ATT CAC SEQ ID NO: 234 CAG CAG CCG CGG TAA SEQ ID NO: 235 ATG TGG TTT AAT TCG SEQ ID NO: 236 GT GCC AGC AGC CGC SEQ ID NO: 237 AAC GAG CGC AAC CC SEQ ID NO: 238 GTG GTT TAA TTC GA SEQ ID NO: 239 ACC TTG TTA CGA CTT SEQ ID NO: 240 CGA CAG AGT TTG ATC SEQ ID NO: 241 ACC TTG TTA CGA CTT SEQ ID NO: 242 CAG CCG CGG TAA TAC SEQ ID NO: 243 AAC AGG ATT AGA TAC SEQ ID NO: 244 GTC GTC AGC TCG TGT SEQ ID NO: 245 ATG TTG GGT TAA GTC SEQ ID NO: 246 GAA TCG CTA GTA ATC SEQ ID NO: 247 CTT GTA CAC ACC GCC SEQ ID NO: 248 GGA CTA CCA GGG TAT CTA AT

TABLE-US-00025 TABLE 25 .gamma.PNA reporter probe sequences for fungi SEQ ID Sequence (N-terminus to C-terminus) SEQ ID NO: 249 GTG AAA CTG CGA ATG SEQ ID NO: 250 CTG CGA ATG GCT CAT SEQ ID NO: 251 GCT CAT TAA ATC AGT SEQ ID NO: 252 TCA GTT ATC GTT TAT SEQ ID NO: 253 CGT TTA TTT GAT AGT SEQ ID NO: 254 TCT AGA GCT AAT ACA SEQ ID NO: 255 TAG AGC TAA TAC ATG SEQ ID NO: 256 TGT ATT TAT TAG ATA SEQ ID NO: 257 TTA TTA GAT AAA AAA SEQ ID NO: 258 TGG TTC ATT CAA ATT SEQ ID NO: 259 TTC AAA TTT CTG CCC SEQ ID NO: 260 CTG CCC TAT CAA CTT SEQ ID NO: 261 AAC TTT CGA TGG TAG SEQ ID NO: 262 TCG ATG GTA GGA TAG SEQ ID NO: 263 AGG GTT CGA TTC CGG SEQ ID NO: 264 AGC CTG AGA AAC GGC SEQ ID NO: 265 AGA AAC GGC TAC CAC SEQ ID NO: 266 CTA CCA CAT CCA AGG SEQ ID NO: 267 ATC CAA GGA AGG CAG SEQ ID NO: 268 AAG GCA GCA GGC GCG SEQ ID NO: 269 AGG CGC GCA AAT TAC SEQ ID NO: 270 CAA ATT ACC CAA TCC SEQ ID NO: 271 GAG GTA GTG ACA ATA SEQ ID NO: 272 GTA ATT GGA ATG AGT SEQ ID NO: 273 TGG AAT GAG TAC AAT SEQ ID NO: 274 CCT TAA CGA GGA ACA SEQ ID NO: 275 CAA GTC TGG TGC CAG SEQ ID NO: 276 TAA TTC CAG CTC CAA SEQ ID NO: 277 AGC GTA TAT TAA AGT SEQ ID NO: 278 TTA AAG TTG TTG CAG SEQ ID NO: 279 GTT GCA GTT AAA AAG SEQ ID NO: 280 AGC TCG TAG TTG AAC SEQ ID NO: 281 AAA TTA GAG TGT TCA SEQ ID NO: 282 GTG TTC AAA GCA GGC SEQ ID NO: 283 ATT AGC ATG GAA TAA T SEQ ID NO: 284 GGT TCT ATT TTG TTG SEQ ID NO: 285 TGT TGG TTT CTA GGA SEQ ID NO: 286 GTC AGA GGT GAA ATT SEQ ID NO: 287 TGA AAT TCT TGG ATT SEQ ID NO: 288 TGA AGA CTA ACT ACT SEQ ID NO: 289 TAC TGC GAA AGC ATT SEQ ID NO: 290 GTT TTC ATT AAT CA SEQ ID NO: 291 AAC GAA AGT TAG GG SEQ ID NO: 292 GAT CAG ATA CCG TCG SEQ ID NO: 293 ACC GTC GTA GTC TTA SEQ ID NO: 294 TAG TCT TAA CCA TAA SEQ ID NO: 295 ACC ATA AAC TAT GCC SEQ ID NO: 296 TAT GCC GACT AGG GAT SEQ ID NO: 297 TCG GCA CCT TAC GAG SEQ ID NO: 298 TAC GAG AAA TCA AAG SEQ ID NO: 299 AGT ATG GTC GCA AGG SEQ ID NO: 300 GGC TGA AAC TTA AAG SEQ ID NO: 301 AGC CTG CGG CTT AAT SEQ ID NO: 302 TTA ATT TGA CTC AAC SEQ ID NO: 303 AAA CTC ACC AGG TCC A SEQ ID NO: 304 TGG AGT GAT TTG TCT SEQ ID NO: 305 TGT CTG CTT AAT TGC GAT SEQ ID NO: 306 AAC AGG TCT GTG ATG SEQ ID NO: 307 TGT GAT GCC CTT AGA SEQ ID NO: 308 GCG CGC TAC ACT GAC SEQ ID NO: 309 TTG CTC TTC AAC GAG

[0059] The present disclosure provides .gamma.PNA probes useful for the timely detection and/or identification of sepsis-inducing pathogens without the need of culturing the clinical specimens. These qualities are specific to the sequences of the optimized probes, however, one of skill in the art would recognize that other molecules with similar sequences could also be used. The .gamma.PNA probes provided herein comprise a sequence that shares at least about 60-70% identity with a sequence described in Tables 1-25. In another embodiment, the .gamma.PNA probe has a sequence that shares at least about 71%, about 72%, about 73%, about 74%, about 75%, about 76%, about 77%, about 78%, about 79%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, or about 99% identity with the sequences of Tables 1-25 or complement thereof. The terms "identity" or "homology" or "similarity" refer to sequence relationships between two .gamma.PNA sequences and can be determined by comparing a nucleotide position in each sequence when aligned for purposes of comparison. The term "identity" refers to the degree to which nucleic acids are the same between two sequences. The term "homology" or "similarity" refers to the relatedness of two functionally-equivalent .gamma.PNA sequences.

[0060] The probe .gamma.PNA sequences also include functional fragments of the sequence provided in Tables 1-25 and sequences sharing certain sequence identities with those in Tables 1-25, as described above, provided they function to specifically anneal to and identify sepsis-inducing pathogens. In one aspect, these fragment sequences have 1, 2, 3, 4, 5, or 6 less bases at either or both ends of the original sequences in Tables 1-25. These shorter sequences are also within the scope of the present disclosure.

[0061] In addition, the .gamma.PNA sequences, including those provided in Tables 1-25 and sequences sharing certain sequence identities with those in Tables 1-25, as described above, can be incorporated into longer sequences, provided they function to specifically anneal to and identify sepsis-inducing pathogens. In one aspect, the longer sequences have 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 additional bases at either or both ends of the original sequences. These longer sequences are also within the scope of the present disclosure.

[0062] The probe .gamma.PNA sequences are complementary to the target nucleic acid sequence. The probe .gamma.PNA sequences of the disclosure are optimal for identifying numerous strains of a target nucleic acid, e.g., Staphylococcus aureus.

Composition of .gamma.PNA Probes

[0063] In one embodiment, .gamma.PNA probes are used in the diagnosis of sepsis. .gamma.PNA probes can be divided into two classes, .gamma.PNA capture probes and .gamma.PNA reporter probes.

PNA Capture Probes

[0064] In some embodiments, the .gamma.PNA capture probes are used for the capture, immobilization, and affinity purification of pathogenic genomic material from the sample. In some embodiments, the .gamma.PNA capture probes are designed such that the probes' sequence binds only to one species of pathogen. In some embodiments, the .gamma.PNA capture probes are designed such that the probes' sequence binds to more than one species of pathogen.

[0065] If targeted genomic material is present in the sample, the genomic material will be captured and immobilized or bound by the .gamma.PNA capture probe. If genomic material is not present; no genomic material will be immobilized or bound by the .gamma.PNA capture probe. The capture and affinity purification of the genomic content does not require the entire genomic fragment to be captured. Rather, a small portion of the target genome being captured constitutes the relevant portion of the genome being captured. In one embodiment, the .gamma.PNA capture probes comprise of .gamma.PNAs having one or more of the probe sequences listed in Tables 1-23.

[0066] The .gamma.PNA capture probes can be modified by one or more of the characteristics listed below. That is, the .gamma.PNA capture probes include, but are not limited to, the following embodiments.

[0067] In some embodiments, the .gamma.PNA capture probe sequences will be pre-immobilized onto a support substrate prior to introducing the sample to be tested. In some embodiments, a "single .gamma.PNA capture probe sequence" will be pre-immobilized onto a predefined location on a support substrate. A "single .gamma.PNA capture probe sequence" is defined as only one sequence for a single microbial pathogenic species, which enables identification and quantification of the target pathogen. For example, a single species .gamma.PNA probe sequence encompassing only one of the probe sequences in Table 1 to enable capture of Staphylococcus aureus.

[0068] In another embodiment, "single .gamma.PNA capture probe sequence set," all sequences to a single pathogen, can be pre-immobilized onto a predefined location on a support substrate enabling identification and quantification of the target pathogen. "Single .gamma.PNA capture probe sequence set" is defined as multiple sequences for a single microbial pathogenic species, which enables identification and quantification of the target pathogen. For example, a single species .gamma.PNA capture probe sequence set encompassing two or more of the probe sequences in Table 1 to enable capture of Staphylococcus aureus.

[0069] In another embodiment, one or more .gamma.PNA capture probe sequences for more than one pathogen can be pre-immobilized onto a predefined location on a support substrate with specificity, enabling identification and quantification of the pathogen subset of interest. For example, multiple .gamma.PNA capture probe sequences encompassing at least one probe sequence from both Table 1 and Table 2 to enable capture of Staphylococcus aureus and Enterococcus faecalis.

[0070] In some embodiments, the .gamma.PNA capture probes sequences in Tables 1-23 include a moiety which enables them to be surface immobilized on a support substrate. Immobilization of the .gamma.PNA capture probes sequences on a support substrate can be achieved through various means such as covalent binding protocols or non-covalent binding protocols. Common binding modalities/chemistries that can be used to immobilize the .gamma.PNA on the support substrate include, but are not limited to, COOH groups, NHS-ester groups, malemide chemistry, Click chemistry, streptavidin, thiol chemistry, and biotinylation. There are multiple additional methods which are commonly known to those skilled in the art.

[0071] In some embodiments the support substrate is coated with Avidin, Neutravidin, or Streptavidin to facilitate the immobilization of the .gamma.PNA capture probes sequences.

[0072] In some embodiments, the support substrate is one or more of the group consisting of a magnetic bead, a bead, a well, a plate, for example a polystyrene microtiter plate, a test tube, a stick, for example dipstick, a plastic, a glass, and a chip or a biochip. In some embodiments, the support substrate is silicon based or coated with either a semiconductive, conductive, or insulating material. In some embodiments, the support substrate includes metallic surfaces that are functionalized. In other embodiments the solid substrate may be manufactured from polymers, nylon, nitrocellulose, polyacrylamide, oxides. In some embodiments, the solid support is manufactured from multiple materials. In some embodiments, the surface of the support substrate is coated with an aminosilane or any other commonly known surface treatments, such as epoxysilanes.

[0073] The .gamma.PNA capture probe lengths are considered to be substantially shorter than those typically used in similar applications due to the enhanced affinity of PNA probes in general and .gamma.PNA probes in particular when compared to DNA and RNA probes. The enhanced affinity is a result of the neutral backbone and the pre-organization due to the .gamma.-modification. Shorter probes in general are advantageous as they offer superior sequence specificity.

[0074] In one embodiment, the .gamma.PNA capture probes have relatively short nucleobase sequences, typically 5-30 bases in length. In another embodiment, the .gamma.PNA capture probes are 12-27 bases in length. In another embodiment, the .gamma.PNA capture probes are 15-24 bases in length. In another embodiment, the .gamma.PNA capture probes are 18-21 bases in length.

[0075] In some embodiments, the .gamma.PNA capture probe may include moieties which add functionality to the probe itself. Examples include, but are not limited to, binding molecules (such as biotin or haptens), spacer groups, linker groups, a hydrophobicity-changing group, a charge-inducing group, and a structural change-inducing group.

[0076] Examples of spacer groups include, but are not limited to, (ethylene)glycol, di(ethylene)glycol, tri(ethylene)glycol, poly(ethylene)glycol, 6-carbon linker, and 12 carbon linker.

[0077] Examples of linker groups include, but are not limited to, COOH group, NETS-ester group malemide chemistry, Click chemistry, streptavidin, and biotinylation.

[0078] Examples of hydrophobicity-changing groups include, but are not limited to, a naturally polar or charged side group or linker that decreases hydrophobicity, such as side groups mimicking those found on Arginine, Histidine, Lysine, Aspartic Acid, Glutamic Acid, Serine, Threonine, Asparagine, and Glutamine, or a naturally apolar and uncharged side group or linker that increases hydrophobicity, such as side groups mimicking those found on Alanine, Valine, Isoleucine, Leucine, Methionine, Phenylalanine, Tyrosine, and Tryptophan.

[0079] Examples of charge-inducing groups include, but are not limited to, COOH group, NH.sub.3 groups, OH groups, and metallic ions.

[0080] Structural change-inducing group induces a chemical modification in the .gamma.PNA capture probe's pseudo-peptide backbone to change the overall charge of the PNA. Typical examples include a selection of positively charged or negatively charged amino acids to thereby alter the charge of the .gamma.PNA capture probe. In addition, small particles, small molecules, amino acids residues, small proteins or otherwise peptides may be incorporated, or conjugated along the backbone to alter the physical characteristics of the .gamma.PNA capture probe, which would serve to either alter the affinity of the molecule or even its sequence specificity. Examples of structural change-inducing groups include, but are not limited to, amino acid-based side chain, nanoparticle, small molecule or intercalating agent.

[0081] In some embodiment, the .gamma.PNA capture probe has an easily identifiable signal induced at the capture site. Common signals include, but shall not be limited to fluorescence, luminescence, FRET, colorimetric, calorimetric, interference patterns, pH, resistance/conductivity, enzymatic function and kinetics, protein structure, and electrical potential. In some embodiments, detection of the presence of one or more targeted genomic material is selected from electrical, mass spectrometry, and/or precipitate.

.gamma.PNA Reporter Probes

[0082] The .gamma.PNA reporter probe is used to establish the presence, or alternatively the absence, of a targeted pathogen. .gamma.PNA reporter probes are designed to bind a conserved sequence region common among all bacteria. The .gamma.PNA reporter probe can be introduced regardless of the presence or absence of the captured genomic material. In one embodiment, the .gamma.PNA reporter probes comprise of .gamma.PNAs having one or more of the probe sequences listed in Tables 24-25. In one embodiment, the purpose of the .gamma.PNA reporter probe is to establish the presence of a target pathogen through the presence of pathogenic genomic material at a particular location. In some embodiments, the purpose of the .gamma.PNA reporter probe is to quantify the amount of target pathogen through its genomic material at a particular location.

[0083] In one embodiment, a single .gamma.PNA reporter probe sequence is used that is common, or universal, to the all of the potential targets. In another embodiment, multiple .gamma.PNA reporter probe sequences may be used where together, as a group, they are universal to all or some of the potential target pathogenic genomic material. In another embodiment, multiple .gamma.PNA reporter probe sequences may be used which may bind to multiple locations along the target genomic material.

[0084] The .gamma.PNA reporter probe lengths are considered to be substantially shorter than those typically used in similar applications due to the enhanced affinity of PNA probes in general and .gamma.PNA probes in particular when compared to DNA and RNA probes. The enhanced affinity is a result of the neutral backbone and the pre-organization due to the .gamma.-modification. Shorter probes in general are advantageous as they offer superior sequence specificity. In some embodiments, the .gamma.PNA reporter probes have relatively short nucleobase sequences, typically 5-30 bases in length. In another embodiment the .gamma.PNA reporter probes have 12-27 bases in length. In another embodiment, the .gamma.PNA capture probes are 15-24 bases in length. In another embodiment, the .gamma.PNA capture probes are 18-21 bases in length.

[0085] The .gamma.PNA reporter probe contains a moiety that induces a signal. In one embodiment, the .gamma.PNA reporter probe has a signal inducing capability selected from, but not limited to fluorophores, quantum dots, enzymes, conjugates, small molecules, chromophore, inorganic nanoparticles (such as metals or semiconductors), conjugation enabling modifications, radioisotopes, and luminescent compounds. In some embodiments, the .gamma.PNA reporter probe is synthesized with a specific chemical moiety, which later enables the conjugation of a signal inducing compounds. Examples of specific chemical moieties, but not limited to, are COOH groups, NETS-ester groups, malemide chemistry, Click chemistry, streptavidin, and biotinylation.

[0086] In some embodiments, the .gamma.PNA reporter probe may include moieties which add functionality to the probe itself. Examples include, but are not limited to, binding molecules (such as biotin or haptens), spacer groups, linker groups, a hydrophobicity-changing group, a charge-inducing group, and a structural change-inducing group.

[0087] Examples of spacer groups include, but are not limited to, (ethylene)glycol, di(ethylene)glycol, tri(ethylene)glycol, poly(ethylene)glycol, 6-carbon linker, and 12 carbon linker.

[0088] Examples of linker groups include, but are not limited to, COOH group, NHS-ester group malemide chemistry, Click chemistry, streptavidin, and biotinylation.

[0089] Examples of hydrophobicity-changing groups include, but are not limited to, a naturally polar or charged side group or linker that decreases hydrophobicity, such as Arginine, Histidine, Lysine, Aspartic Acid, Glutamic Acid, Serine, Threonine, Asparagine, and Glutamine, or a naturally apolar and uncharged side group or linker that increases hydrophobicity, such as side groups mimicking those found on Alanine, Valine, Isoleucine, Leucine, Methionine, Phenylalanine, Tyrosine, and Tryptophan.

[0090] Examples of charge-inducing groups include, but are not limited to, COOH group, NH.sub.3 groups, OH groups, and metallic ions.

[0091] Structural change-inducing group induces a chemical modification in the .gamma.PNA reporter probe's pseudo-peptide backbone to change the overall charge of the PNA. Typical examples include a selection of positively charged or negatively charged amino acids to thereby alter the charge of the .gamma.PNA reporter probe. In addition, small particles, small molecules, amino acids residues, small proteins or otherwise peptides may be incorporated, or conjugated along the backbone to alter the physical characteristics of the .gamma.PNA reporter probe, which would serve to either alter the affinity of the molecule or even its sequence specificity. Examples of structural change-inducing groups include, but are not limited to, amino acid-based side chain, nanoparticle, small molecule or intercalating agent.

[0092] However, in some embodiments, the .gamma.PNA reporter probe is not required. Rather, signal inducing agents such as DNA/RNA intercalating dyes, which can induce signals themselves, can be used. Several different intercalating dyes are known, such as ethidium bromide and SYBR Green. These dyes are well established and usage of them is well known to those skilled in the art.

[0093] In some embodiments, where the .gamma.PNA reporter probe is not required, a target pathogen may be PCR amplified with one or more of the primers containing fluorophores, quantum dots, enzymes, conjugates, small molecules, chromophore, inorganic nanoparticles (such as metals or semiconductors), conjugation enabling modifications, radioisotopes, and luminescent compounds or with a specific chemical moiety, which later enables the conjugation of a signal inducing compounds. Examples of specific chemical moieties, but not limited to, are COOH groups, NETS-ester groups, malemide chemistry, Click chemistry, streptavidin, and biotinylation. These methods are well established and known to those skilled in the art.

Carbon Linkers and Biotinylation

[0094] Carbon linkers serve different purposes depending on which type of .gamma.PNA probe they are attached. .gamma.PNA capture probe utilize carbon linkers to remove issues due to potential steric hindrance between the surface of the support substrate and pathogenic genomic material.

[0095] In one embodiment, the .gamma.PNA capture probes' carbon linkers comprises of at least one carbon. In another embodiment, the .gamma.PNA capture probes' carbon linkers comprises of 1-100 carbons. In another embodiment, the .gamma.PNA capture probes' carbon linkers comprises of 1-50 carbons. In another embodiment, the .gamma.PNA capture probes' carbon linkers comprises of 1-25 carbons. In another embodiment, the .gamma.PNA capture probes' carbon linkers comprises of 5-15 carbons.

[0096] .gamma.PNA reporter probe utilizes carbon linkers to eliminate issues of steric hindrance between the .gamma.PNA reporter probe and its signaling moiety. In another embodiment, the .gamma.PNA reporter probes' carbon linkers comprises of 1-100 carbons. In another embodiment, the .gamma.PNA reporter probes' carbon linkers comprises of 1-50 carbons. In another embodiment, the .gamma.PNA reporter probes' carbon linkers comprises of 1-25 carbons. In another embodiment, the .gamma.PNA reporter probes' carbon linkers comprises of 5-15 carbons.

[0097] The signal expressed by .gamma.PNA reporter probes can be amplified by having multiple biotinylation sites. In one embodiment, the .gamma.PNA reporter probes' carbon-linker comprises of one or more biotinylation sites.

Methods for Diagnosing Sepsis Using .gamma.PNA Probes

[0098] In another embodiment, .gamma.PNA probes provide methods for diagnosing bacterial and fungal pathogens which induce sepsis.

[0099] In one embodiment, a .gamma.PNA capture probe, comprising one or more of the above mentioned .gamma.PNA capture probe compositions, is combined and incubated with a sample from a person who is suspected of having sepsis. During the incubation, the .gamma.PNA capture probes will bind any genomic material (dsDNA, ssDNA, or RNA) with the target sequence. In some embodiments, the mixture of .gamma.PNA capture probes and sample is heated to facilitate invasion and binding of the .gamma.PNA capture probes to target genomic sequences.

[0100] In one embodiment, the method may consist of DNA amplification, for example through PCR, of the genomic material in the sample.

[0101] In one embodiment, the genomic material in the sample is sheared. "Shearing" refers to shortening dsDNA, ssDNA, or RNA strands. Shearing circumvents issues with DNA/RNA knotting/supercoiling due to the length of the bacterial genomic material. In some embodiments, the genomic material is sheared to at least 10 kbp strands. In some embodiments, the genomic material is sheared to about 10-500 bp. In other embodiments, the genomic material is sheared to 250-2,000 bp. In other embodiments, the genomic material is sheared from 1,000-10,000. In another embodiment, the genomic material is sheared to 5,000-50,000 bp. Shearing is well-known in the art and commercial kits are widely available.

[0102] In one embodiment, identification of the absence or presence of a particular microbe through its genomic material requires a binary result of capture or non-capture. In some embodiments, quantification of the load or copy number of pathogenic genomic material present in the sample can be correlated to the number or amount of pathogenic genomic material captured via the .gamma.PNA capture probe.

[0103] In one embodiment, a wash step is performed after incubation of the .gamma.PNA capture probes and patient sample. Washing steps minimize unwanted, unbound, weakly bound, non-specifically bound target and other non-target material from the vicinity of the .gamma.PNA capture probes. Washing steps are well established and known to those skilled in the art.

[0104] The capture of the genomic content does not enable identification by itself. Rather a detectable signal must be induced at the capture site. In one embodiment, the .gamma.PNA capture probe induces a signal upon binding to the target sequence. The induced signal can be selected from, but not limited to, fluorescence, luminescence, FRET, colorimetric, calorimetric, interference patterns, pH, resistance/conductivity, enzymatic function and kinetics, protein structure, and electrical potential.

[0105] In alternate embodiment, after the affinity purification of the target genomic material by .gamma.PNA capture probe, a .gamma.PNA reporter probe, comprising one or more of the above mentioned .gamma.PNA reporter probe compositions, is introduced to the system. The .gamma.PNA reporter probe "invades" the immobilized target genomic material. In some embodiments the .gamma.PNA reporter probe contains a moiety which simplifies detection of a signal. In some embodiments, the .gamma.PNA reporter probe is synthesized with such a signal inducing capability, which include, but not limited to: fluorophores, quantum dots, enzymes, fluorescence, FRET, absorption, raman and/or SERS, chemiluminescence, bioluminescence, and scattering.

[0106] In some embodiments, the .gamma.PNA reporter probe is synthesized with a specific chemical moiety, which later enables the conjugation of a signal inducing compounds. Examples of specific chemical moieties, but not limited to, are: COOH groups, NETS-ester groups, malemide chemistry, Click chemistry, streptavidin, and biotinylation.

[0107] In some embodiments, the method of detecting .gamma.PNA reporter probe binding to the target is selected from, but not limited to, electrical, mass spectrometry, and/or precipitate.

[0108] In some embodiments, a wash step is performed to remove loosely bound .gamma.PNA reporter probes. These steps remove unwanted, unbound, weakly bound, non-specifically bound .gamma.PNA reporter probes from the system.

[0109] In some embodiments, the distance, in base pairs or bases, between the .gamma.PNA capture probe and .gamma.PNA reporter probe should be optimized to reduce the likelihood of DNA/RNA breakage between the two binding sites. The distance between the two probes should be sufficient such that the invasion process is not hindered. In some embodiments, the probe sites, or target regions, are between about 10 to 100,000 bases apart. In another embodiment, the probe sites, or target regions, are between about 50 to 75,000 bases apart. In another embodiment, the probe sites, or target regions, are between about 100 to 50,000 bases apart. In another embodiment, the probe sites, or target regions, are between about 10,000 to 100,000 bases apart.

[0110] In some embodiments, the .gamma.PNA capture probes sequences do not identify a specific species, but can identify a group of species. For example, Coagulase-Negative staphylococci (CoNS) encompasses a group of Staphylococci species, which includes, but is not limited to, Staphylococcus capitis, Staphylococcus cohnii, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus lugdunensis, Staphylococcus saprophyticus, Staphylococcus warneri, Staphylococcus capitis. A pool of .gamma.PNA capture probe sequences can be used to identify the CoNS group, see Table 18. For example, if the use of Pool 1 generates a positive signal, the signal indicates that one or more of the above CoNS species is present. In some embodiments, the .gamma.PNA capture probes sequences are drawn from one or more pools from Table 18.

Kit for Diagnosing Sepsis Using .gamma.PNA

[0111] In another embodiment, .gamma.PNA probes are used in kits for diagnosis or detection of sepsis or determining the quantity of sepsis related genomic material using .gamma.PNA. In one embodiment, the kit comprises a plurality of .gamma.PNA capture probes, wherein the .gamma.PNA capture probes comprise a sequence or the reverse-complementary sequence selected from one or more of the sequences from Tables 1-23. The .gamma.PNA capture probes having any of the characteristics or conjugates previously described. In some embodiments, the kit also comprises a plurality of .gamma.PNA reporter probes, wherein the .gamma.PNA reporter probes comprise a sequence or the reverse-complementary sequence selected from one or more sequences form Tables 24-25, the .gamma.PNA reporter probes having any of the characteristics or conjugates previously described.

EXEMPLIFICATION

[0112] The following examples describe embodiments, which are merely illustrative and should not be construed as limiting in any way.

Example 1. Exemplary Overview

[0113] The following steps are a general overview of methods and material for one embodiment using .gamma.PNA probes to identify bacteria species in a sample.

Step I: Loading Magnetic Beads with Capture Probes

[0114] With reference to FIG. 1A, a solution of excess capture probes 103 is incubated with Neutravidin coated 102 magnetic beads 101. The biotinylated .gamma.PNA capture probes naturally bind to the Neutravidin coated beads owing to the very high affinity of biotin to Neutravidin, which immobilizes the .gamma.PNA capture probes on the magnetic bead 104. After binding capture probes on the beads, excess probes are washed away using magnetic separation.

[0115] After rinsing, the beads are washed with solution of free biotin to block the remaining unoccupied sites on the beads.

Step II: .gamma.PNA-Mediated Magnetic Extraction of Species-Specific DNA from a Mixture

[0116] With reference to FIG. 1B, a clinical sample of a patient with both target DNA genomes 106 and non-targeted DNA genomes 107 is mixed with the magnetic beads with bound .gamma.PNA capture probes 105 and heated at 50.degree. C. The .gamma.PNA capture probes capture and immobilize the specific species target onto the bead 108, if and only if the corresponding sequence is present in the sample. Extraction of the target DNA is then accomplished by magnetically capturing the beads, and washing away the un-captured background DNA.

Step III: Binding of .gamma.PNA Reporter Probes onto the Affinity Purified Targets

[0117] With reference to FIG. 1C, the magnetic beads with bound target DNA 109 are re-suspended in a 50.degree. C. solution with a large excess of the .gamma.PNA reporter probes 110. After .gamma.PNA reporter probes bind to the target 111, magnetic separation is used to wash away unbound .gamma.PNA reporter probes. If no target was present in the original sample, then no reporter probes will remain in this step. Each probe is specific to a particular pathogen of interest, but is designed to bind a conserved sequence region, independent of the pathogen's subtypes.

Step IV: Binding of Reporter Enzyme onto Captured Targets

[0118] With reference to FIG. 1D, a commercial Neutravidin HRP conjugate (ThermoScientific) 113 is mixed with the magnetic beads, which have bound target DNA labeled with .gamma.PNA reporter probes 112. The HRP conjugate binds to the biotinylated .gamma.PNA reporter probe 114. Unbound enzymes are rinsed away using magnetic separation.

Step V: Reporter Substrate Addition and Optical Readout

[0119] With reference to FIG. 1E, a chemiluminescent HRP substrate 116 from a commercial ELISA kit (SuperSignal ELISA Femto, ThermoScientific) is mixed with the magnetic beads, which contain HRP conjugates 115. The HRP substrate is used to produce light signals indicating 117 the presence of a target in a sample.

[0120] Additional optical methods of detection include, but are not limited to, fluorescence, FRET, quantum dots, absorption, raman and/or SERS, chemiluminescence, bioluminescence, and scattering. Other detection methods include, but are not limited to, mass spectrometry and/or precipitate. Support substrates include but are not limited to any well based, dipstick, flow methods, chip, glass, bead, silicon, fibers, and/or paper.

Example 2. Identification of Staphylococcus aureus from a Clinical Sample

[0121] Staphylococcus aureus is detected in a clinical sample by adding the sample to a well or wells on a 96-well microplate, wherein each well is coated with .gamma.PNA capture probes specific to Staphylococcus aureus. If Staphylococcus aureus is present in the sample, a signal is detected that indicates possible sepsis infection in the patient. In an alternative embodiment, positive and negative control samples are also tested along with the clinical sample. The positive and negative control samples are also added to wells coated with .gamma.PNA capture probes specific to Staphylococcus aureus. The positive control is a sample known to have Staphylococcus aureus. The negative control sample is known not to have Staphylococcus aureus.

Materials and Methods

[0122] Streptavidin coated wells in 96-well microplates (Kaivogen Oy, Finland) are used as a solid support substrate. The microplate is pre-activated with .gamma.PNA capture probe(s), wherein the sequence of each capture probe is selected from one or more sequences from Table 1, which are sequences specific to Staphylococcus aureus. .gamma.PNA capture probes are synthesized (PNA Innovations, Inc., USA) to contain a biotin moiety on its N-terminus, which enables binding of the .gamma.PNA capture probes to the microplate well. Post-binding, the well is blocked with a biotin wash, which saturates all remaining biotin binding sites in the well. Post-blocking, the wells are thoroughly rinsed with a 0.2 micron filtered 10 mM NaPi buffer (pH 7.0).

[0123] DNA from a clinical patient sample are isolated using a Wizard Genomic Extraction Kit (Promega, Inc., USA). After DNA isolation, the DNA is sheared to a uniform length such as 10 kbp using a `G-Tube` (Covaris, Inc. USA). Typical shearing protocol includes centrifuging extracted DNA sample for 60 sec at 8 krpm in an Eppendorf Minispin microcentrifuge (Eppendorf AG, Germany). Next, the DNA sample is concentrated and added to the .gamma.PNA capture probe-activated well. To promote .gamma.PNA capture probe invasion into the genomic target the well is heated to 60.degree. C. for 30 minutes in 10 mM NaPi (pH 7.0) with 15 mM NaCl, 0.05% Tween-20. After invasion, the sample is washed to remove uncaptured DNA from the well. After the wash, .gamma.PNA reporter probes, which can also be biotinylated, are added to the well in 10 mM NaPi (pH 7.0) with 50 mM NaCl, 0.1% Tween to a final concentration of 1 uM. The .gamma.PNA reporter probes sequence is selected from one or more sequences from Table 24. The well is heated using the afore mentioned protocol and then washed to remove all unbound .gamma.PNA reporter probes.

[0124] Streptavidin conjugated HRP (VectorLabs, Inc., USA) is added to a final concentration of 1 ng/ml to each microplate well and incubated at room-temperature for 30 min. Post-incubation, each well is washed to remove unbound Streptavidin conjugated HRP. Next, a substrate for HRP, such as SuperSignalFemto (Thermo-Scientific, USA), is added to each well and the emitted optical signal is read on a luminescence plate reader (GloMax 96, Promega, Inc., USA). The presence of Staphylococcus aureus in the clinical sample is indicated by an emitted optical signal.

Example 3: Determining if an Infection Arises from Coagulase Negative Staphylococci (CoNS) Or from Staphylococcus aureus

[0125] In one embodiment, .gamma.PNA probes are used to identify if a clinical sample is infected with a CoNS species or Staphylococcus aureus. The clinical sample originates from an individual who is deemed to have a possible blood-borne infection. Staphylococcus aureus or a CoNS species are detected in the sample by adding the sample to wells on a 96-well microplate, wherein some wells are coated with .gamma.PNA capture probes specific to Staphylococcus aureus and other wells are coated with .gamma.PNA capture probes specific to CoNS species. A detectable signal in Staphylococcus aureus and/or CoNS species coated wells is indicative of the presence of that bacteria or bacterial family.

[0126] In an alternative embodiment, positive and negative control samples are also tested along with the clinical sample. The positive and negative control samples are also added to wells coated with .gamma.PNA capture probes specific to Staphylococcus aureus or .gamma.PNA capture probes specific to CoNS species. The positive controls are samples known to have Staphylococcus aureus and/or CoNS species. The negative control sample is known not to have neither Staphylococcus aureus and CoNS species.

Methods and Materials

[0127] As previously described in Example 2, a 96-well microplate pre-coated with Streptavidin is used as a solid support substrate. Two different .gamma.PNA capture probes or sets of capture probes (PNA Innovations, Inc., USA) are added to one or more separate wells. The first well or set of wells contain .gamma.PNA capture probes having one or more sequences selected from Table 1, identified as CoNS-. The second well or set of wells, identified as CoNS+, has .gamma.PNA capture probes with sequences selected from one of the pools listed in Table 18. The sequences within the pool are pre-mixed at equal-molar concentrations. The sequences found in each pool in Table 18 are unique to a number of CoNS species, which include, but is not limited to, Staphylococcus capitis, Staphylococcus cohnii, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus lugdunensis, Staphylococcus saprophyticus, Staphylococcus warneri, Staphylococcus capitis. The .gamma.PNA capture probes are incubated on support substrate for 30 minutes to ensure proper binding. After binding of .gamma.PNA capture probes to the wells is completed, the wells are blocked with a biotin wash to saturate remaining biotin binding sites. Post-blocking, the wells are rinsed with a 0.2 micron filtered 10 mM NaPi buffer (pH 7.0).

DNA Amplification

[0128] DNA from a clinical patient sample is isolated using a Wizard Genomic Extraction Kit (Promega, Inc., USA). After DNA isolation, the DNA sample is concentrated and amplified using Broad-Range PCR (specific to the 16s region of bacteria) using Phusion DNA polymerase (New England Biolabs, Inc., USA).

[0129] The following primers are used for DNA amplification: Forward Primer Sequence: 5' AGA GTT TGA TCC TGG CTC AG (SEQ ID NO: 310); Reverse Primer Sequence: 5' ATC GGC TAC CTT GTT ACG ACT TC (SEQ ID NO: 311).

[0130] Both primers are mixed in equal-molar concentrations to a final concentration of 0.5 uM. dNTPs are added, likewise in equal-molar concentrations, to a final concentration of 200 uM each. Phusion is added to a final concentration of 2 u/100 ul with the recommend buffer and DNase/RNase-free water.

[0131] Thermocycling conditions are as follows:

[0132] 1) 45 seconds at 98.degree. C..times.1 cycle

[0133] 2) (15 seconds at 98.degree. C., 30 seconds at 55.degree. C., 75 seconds at 72.degree. C.).times.35 cycles

[0134] 3) 5 minutes at 72.degree. C..times.1 cycle

[0135] Post-PCR processing, the amplified 16s region of the bacterial pathogen is isolated using the DNA Clean-uo kit (DNA Clean and Concentrator--5, Zymo Research, Inc., USA). After completing the DNA clean-up process, biotinylated .gamma.PNA reporter probes (PNA Innovations, Inc., USA) with one or more sequence from Table 24 are added to the sample at a final concentration of 1.5 uM in 10 mM NaPi (pH 7.0) and heated for 30 min at 60.degree. C. The biotinylated .gamma.PNA reporter probes will invade the bacterial 16s regions. Human DNA cannot be invade as it contains no 16s region.

.gamma.PNA Capture Probe Invasion Protocol

[0136] The sample is divided and added to both the CoNS- and the CoNS+ wells and incubated with 10 mM NaPi (pH 7.0) with 5 mM NaCl, 0.05% Tween-20 and heated for 30 min to 60.degree. C. Upon completion of the incubation process, both wells are thoroughly rinsed to remove any unbound/uncaptured DNA from each well.

Streptavidin Conjugated HRP Protocol

[0137] Streptavidin conjugated HRP (VectorLabs, Inc., USA) is added to a final concentration of 0.75 ng/ml to each well and incubated at room-temperature for 30 min. The Streptavidin conjugated HRP binds to the open biotin site displayed on the .gamma.PNA reporter probe. Post incubation, the wells are washed to remove unbound Streptavidin conjugated HRP from the well. Finally, a suitable substrate for HRP, such as SuperSignalFemto (Thermo-Scientific, USA) is added to each well and the emitted optical signal is read on a luminescence plate reader (GloMax 96, Promega, Inc., USA).

Results

[0138] If the clinical sample originally contained one or more CoNS species, the CoNS+ well produces a readily detectable optical signal. If the clinical sample originally contained Staphylococcus aureus, the CoNS- well produces a readily detectable optical signal. In the case where neither one or more CoNS pathogens or Staphylococcus aureus is present in the clinical sample, then both wells remain dark. Likewise, if one or more CoNS species are present in addition to Staphylococcus aureus, both the CoNS- and CoNS+ wells emit an optical signal, which is readily detectable. In an alternative embodiment, when an optical signal is produced from the sample, the signal is compared to the positive and negative control samples to determine whether the signal indicates the presence of Staphylococcus aureus and/or CoNS species.

Example 4: Discrimination of an Infection Arising from Two Enterococcal Species: Enterococcus faecalis and Enterococcus faecium

[0139] This example demonstrates the ability of .gamma.PNA probes to differentiate between two species of bacteria that belong to the same genus. A first sample, which serves as a negative control, is produced by a healthy subject, i.e. does not contain pathogens. The second sample is from an subject suspected of having a possible blood-borne infection. In an alternative embodiment, positive control samples are also tested. The positive controls are a sample or samples known to have Enterococcus faecalis and/or Enterococcus faecium.

Methods and Materials

[0140] Similar to the protocol of Example 2, a 96-well microplate is pre-coated with Streptavidin. In this example, two sets of two or more individual wells are coated with different .gamma.PNA capture probe, which have been synthesized to contain a biotin moiety on its N-terminus. In the first set of wells, .gamma.PNA capture probes with one or more sequences from Table 2 are introduced into the wells, identified as E. faecalis. In the second sets of wells, .gamma.PNA capture probes with one or more sequences from Table 3 are introduced into the well, identified as E. faecium. Binding protocol, posting binding blocking, and wash steps from Example 3 will be applied.

[0141] The two samples will be subject to the DNA amplification process and post-PCR processing of Example 3.

[0142] Each sample is added to at least one well in each set. All wells will be subjected to the .gamma.PNA capture probes invasion protocol and excess DNA wash protocol described in Example 3.

[0143] After completing the excess DNA wash protocol, biotinylated .gamma.PNA reporter probes with one or more sequence from Table 24 are added to each well at a final concentration of 1.5 uM in 10 mM NaPi (pH 7.0) and heated for 30 min at 60.degree. C. After incubation the wells are washed to remove excess .gamma.PNA reporter probes.

[0144] Next the wells are subjected to the Streptavidin conjugated HRP protocol detailed in Example 3.

[0145] The negative control wells for both E. faecalis and E. faecium should not yield an optical signal, beyond that which is expected for a sample which contains only non-target genomic material. If however, the clinical sample originally contained pathogens from the bacterial species Enterococcus faecalis, the E. faecalis wells produce a clearly identifiable optical signal, whereas the E. faecium wells do not produce a clearly identifiable optical signal. The reverse would be true if the patient sample originally contained pathogens from the bacterial species Enterococcus faecium. Additionally, if the patient is infected by a bacterial of a different species than Enterococcus faecalis or Enterococcus faecium, or by another pathogen, such as fungal or viral, both well types do not produce a positive readout signature.

Example 5: Discrimination of an Infection Arising from Candida Species or Aspergillus Species Fungal Pathogens Using Magnetic Beads

[0146] This example a clinical sample is tested for two different species of bacteria using .gamma.PNA capture probes immobilized on a magnetic bead. A negative control, as described in Example 4, is included to determine the presence of the bacterial species in the clinical sample. The clinical sample is from an individual who is deemed to have a possible blood-borne infection that may be caused by Candida or Aspergillus. In an alternative embodiment, positive control samples are also tested. The positive controls are a sample or samples known to have Candida and/or Aspergillus.

Methods and Materials

[0147] In this embodiment, magnetic beads that have been pre-activated with amine active sites (DynaBeads, M-270 Amine, Inivtrogen, USA) are initialized for the capture of either Candida or Aspergillus. To produce Candida specific magnetic beads, .gamma.PNA capture probes incorporating equal-molar concentrations of probes specific to target one or more sequences in Tables 19-22 are covalently bound to the magnetic beads utilizing the manufacturer's standard protocol. Likewise, magnetic beads specific to Aspergillus are produced by covalently binding the .gamma.PNA capture probes specific to one or more target sequences in Table 23 according to the manufacturer's standard protocol. In both cases, this captures the .gamma.PNA capture probes to the surface through their C-terminus. Each functionalized bead set is then placed into a separate microcentrigue tubes; one for the control sample, one for Candida species, and one for Aspergillus species

[0148] The two samples are subjected to the DNA amplification process and post-PCR processing discussed in Example 3. The following primers are used for DNA amplification:

TABLE-US-00026 Forward Primer Sequence: (SEQ ID NO: 312) 5' AAA TCA GTT ATC GTT TAT TTG ATA GT; Reverse Primer Sequence: (SEQ ID NO: 313) 5' ATT CCT CGT TGA AGA GCA A.

[0149] The amplified genomic samples are added to their respective microcentrifuge tubes and incubated for 10 min at 80.degree. C. for 10 min in 10 mM NaPi (pH 7.0) with 15 mM NaCl, 0.1% Tween-20. Upon completion of the incubation process, the magnetic beads are captured into a well or set of wells by a rare earth magnet. The well or sets of wells are rinsed to remove any unbound/uncaptured DNA from each magnetic bead. Post capturing targeted 18s regions, biotinylated .gamma.PNA reporter probes with one or more sequence from Table 25 are added to the magnetic beads at a final concentration of 2 .mu.M in 10 mM NaPi (pH 7.0) with 5 mM NaCl, 0.1% Tween-20 and heated at 75.degree. C. for 15 min. After this incubation process, as before, the sample is rinsed/washed through magnetic bead immobilization.

[0150] The magnetic beads are then subjected to the Streptavidin conjugated HRP protocol detailed in Example 3.

Results

[0151] The optical density from negative control magnetic beads will be negligible and acts a baseline for comparison with the clinical sample. If the clinical sample contained pathogens from Candida, the magnetic beads that were functionalized with .gamma.PNA specific to Candida would yield in increased absorbance compared to the control. If the clinical sample contained pathogens from Aspergillus, the magnetic beads that were functionalized with .gamma.PNA specific to Aspergillus would yield in increased absorbance. If the clinical sample was negative for both Candida and Aspergillus, then both magnetic bead sets would have an optical density measurement similar to the negative control.

Example 6: Identification of Escherichia coli from a Clinical Sample

[0152] A solid-support substrate contains the ability to specifically bind and capture a pathogenic genomic target of interest. To achieve this, common glass slides, which have been carboxylated (Xantec Bioanalytics, Germany), are utilized. The glass slides are pre-activated by spotting .gamma.PNA capture probes with one or more sequences from Table 4, which is specific to E. coli. Binding of the .gamma.PNA capture probes to the glass slide is achieved through the N-terminus of the probe and accomplished according to the manufacturer's protocol utilizing 750 nM .gamma.PNA capture probe. Post binding, the glass slides are rinsed with a 0.2 micron filtered 10 mM NaPi buffer (pH 7.0).

[0153] DNA from a clinical patient sample and a healthy patient (serving as a negative control) are isolated and amplified according to the method described in Example 3. The amplified DNA sample is added to the .gamma.PNA capture probe spots on the glass slide. The DNA invasion process is performed under the following conditions; 10 mM NaPi (pH 7.0) with 5 mM NaCl, 0.1% Tween-20, heated to 80.degree. C. for 10 min. Post DNA invasion, the sample is washed. After the wash process, a DNA intercalating dye, Quant-iT PicoGreen (Life Technologies, USA) is added to the spotted samples on the glass slide and incubated at room temperature in a dark room for 20 min. Post incubation, the slide is washed thoroughly to remove non-intercalated dye.

[0154] After washing, the glass slide is imaged using an iXon EM-CCD camera (Andor Technology, UK) coupled with a 525 nm long-pass filter (Edmund Optics, USA), where the slide is excited via a 488 nm CW source (Coherent, USA). An optical signal attained from the spot where the .gamma.PNA capture probe was initially immobilized would indicate the presence of E. coli in the clinical sample.

Example 7: Identification and Quantification of Staphylococcus epidermidis from a Clinical Sample

[0155] In some embodiments, .gamma.PNA probes are used to identify a pathogen and determine the relative concentration of the pathogen in the clinical sample.

[0156] Similar to that which was previously described, a 96-well microplate which pre-coated with Streptavidin. A single individual well or a set of wells contain .gamma.PNA capture probes, which have been synthesized to contain a biotin moiety on its N-terminus sequence. The .gamma.PNA capture probes have one or more sequences selected from Table 5, which specifically target Staphylococcus epidermidis. The .gamma.PNA capture probe binding protocol of Example 3 is applied.

[0157] Known concentrations of Staphylococcus epidermidis (attained via ATCCA) is added to a pathogen-free sample. A calibration curve is created by using eight different known concentration samples, ranging from 10.degree. to 10.sup.7 CFU/ml. After adding Staphylococcus epidermidis to the sample, genomic DNA is extracted using a Wizard Genomic Extraction Kit (Promega, Inc., USA).

[0158] The eight samples will be subject to the DNA amplification process and post-PCR processing of Example 3.

[0159] After completing the DNA clean-up process, biotinylated .gamma.PNA reporter probes with at least three different sequences from Table 24 are added into the sample at a final concentration of 2 uM (per each sequence) in 10 mM Tris-HCl (pH 7.4) 0.05% and heated for 15 min to 75.degree. C. Multiple .gamma.PNA reporter probes serve to amplify the number of active sites introduced into the 16s region. This incubation process is done for each of the eight known concentration samples, individually, in .gamma.PNA capture probe pre-activated wells. Upon completion of the incubation process, all wells are rinsed to remove any unbound/uncaptured DNA or PNA from each well.

[0160] Next, Streptavidin conjugated HRP (VectorLabs, Inc., USA) is added to a final concentration of 1.5 ng/ml to each of the microplate wells and incubated at room-temperature for 15 min. The Streptavidin conjugated HRP binds to the open biotin site displayed on the .gamma.PNA reporter probes. Post incubation, the wells are washed to remove unbound Streptavidin conjugated HRP from the well. Finally, a suitable substrate for HRP, such as SuperSignalFemto (Thermo-Scientific, USA) is added to each well and the emitted optical signal is read on a luminescence plate reader (GloMax 96, Promega, Inc., USA). The intensity signal is then plotted verse the known concentration which the clean sample was spiked with. This also serves to identify the saturation point of the system and likewise the limit of detection of the system. Negative controls of just the clean sample, which followed the same protocol as the eight known samples, are used to estimate the background signal.

[0161] DNA from the clinical patient sample is extracted and isolated using a Wizard Genomic Extraction Kit (Promega, Inc., USA).

[0162] DNA is concentrated and the 16s bacterial region is amplified using Broad-Range PCR (specific to the 16s region of bacteria) with the following protocol with Phusion DNA polymerase (New England Biolabs, Inc., USA):

[0163] The sample is subjected to the DNA amplification process and post-PCR processing of Example 3.

[0164] After completing the DNA clean-up process, an optical signal from the clinical sample is generated using the same protocol used on the eight known samples, discussed above.

[0165] The attained optical signal can then be compared to the previously produced calibration curve, thereby enabling an estimation of the pathogen load of Staphylococcus epidermidis.

Example 8: Gel Shift Assay Demonstrating Binding of Staphylococcus aureus .gamma.PNA Probes to Target Genomic Material

[0166] The specificity of .gamma.PNA probes designed to target Staphylococcus aureus was demonstrated by mixing Staphylococcus aureus targeted .gamma.PNA probes with either a known sample having Staphylococcus aureus genomic material or a known sample negative for Staphylococcus aureus genomic material (non-target genomic material). Binding of the .gamma.PNA probes to Staphylococcus aureus genomic material and lack of binding to non-target genomic material was measured by gel shift assays.

Methods and Materials

[0167] .gamma.PNA capture probes having sequences that target SEQ ID NO: 7, which targets Staphylococcus aureus, were incubated with either a sample of Staphylococcus aureus genomic material or a sample having non-target genomic material.

[0168] .gamma.PNA reporters probes having sequences that target SEQ ID NO: 232, which targets a conserved sequence region common among all bacteria, were incubated with either a sample of Staphylococcus aureus genomic material or a sample having non-target genomic material.

[0169] A control sample of Staphylococcus aureus genomic material without incubation with .gamma.PNA probes was included in the assay. See FIG. 2.

[0170] The Staphylococcus aureus genomic material was .about.350 bp DNA fragments that were amplified from Staphylococcus aureus (ATCC #43300).

[0171] After incubation, the samples were run on a 8% non-denaturing PAGE. The gel was stained for DNA using SybrSafe genomic material intercalating stain.

Results

[0172] As shown in FIG. 2, .gamma.PNA capture probes having sequences targeting SEQ ID NO: 7 were bound to Staphylococcus aureus genomic material after incubation (Lane 2), as indicated by the band shift of Lane 2 when compared to Lane 4, which was Staphylococcus aureus genomic material without incubation with .gamma.PNA probes. Furthermore, Lane 3, which was .gamma.PNA capture probes incubated with non-target genomic material, did not show a shift when compared to Lane 4. The shift in Lane 2 and lack of shift in Lane 3, indicates that the .gamma.PNA capture probes were bound specifically to the Staphylococcus aureus genomic material.

[0173] Similar results were seen with the .gamma.PNA reporters probes having sequences targeting SEQ ID NO: 232. The .gamma.PNA reporters probes also bound specifically to Staphylococcus aureus genomic material (FIG. 2, Lane 5). Lane 5, which contained .gamma.PNA reporters probes incubated with Staphylococcus aureus genomic material, shifts when compared to Lane 4. Additionally, .gamma.PNA reporters probes incubated with non-target genomic material, Lane 6, does not shift, as compared to Lane 4. Thus, .gamma.PNA reporters probes bound specifically to Staphylococcus aureus genomic material.

Example 9: Gel Shift Assay Demonstrating the Sequence Specificity of Staphylococcus aureus .gamma.PNA Capture Probes

[0174] The specificity of .gamma.PNA capture probes was demonstrated by comparing the binding of .gamma.PNA capture probes targeting Staphylococcus aureus to a sample known to have Staphylococcus aureus genomic material or to a sample known to have Staphylococcus epidermidis genomic material.

Materials and Methods

[0175] .gamma.PNA capture probes with sequences targeted at SEQ ID NO: 7, which targets Staphylococcus aureus, was combined with a sample that was know to have Staphylococcus aureus genomic material. The Staphylococcus aureus genomic material was obtained by PCR amplification of the 16s region of Staphylococcus aureus DNA. Staphylococcus aureus genomic material not incubated with .gamma.PNA capture probes was used as a control.

[0176] .gamma.PNA capture probes with sequences targeted at SEQ ID NO: 7 was also combined with a sample that was know to have Staphylococcus epidermidis genomic material. The Staphylococcus epidermidis s genomic material was obtained by PCR amplification of a portion of the 16s region of Staphylococcus epidermidis DNA. This portion of the 16s region of Staphylococcus epidermidis differs from the 16s region of Staphylococcus aureus by a 2 bp mismatch (indicated by underline in FIG. 3). Staphylococcus epidermidis genomic material not incubated with .gamma.PNA capture probes was used as a control.

Results

[0177] Referring to FIG. 3, only Lane 2, which contains .gamma.PNA capture probes incubated with Staphylococcus aureus genomic material, showed a shift when compared to Lane 1 and 3, both contain Staphylococcus aureus genomic material not incubated with .gamma.PNA capture probes. The shift indicates that the .gamma.PNA capture probes were bound to the Staphylococcus aureus genomic material. Conversely, the .gamma.PNA capture probes incubated with Staphylococcus epidermidis genomic material, Lane 4, did not show a shift when compared to Lanes 1 and 3. The lack of a shift in Lane 4 indicates that the .gamma.PNA capture probes specifically targets Staphylococcus aureus.

[0178] The specificity of the .gamma.PNA capture probes was demonstrated as a 2 bp mismatch prevented the Staphylococcus aureus target .gamma.PNA capture probes from binding to the Staphylococcus epidermidis 16s region.

Other Embodiments

[0179] Other embodiments will be evident to those of skill in the art. It should be understood that the foregoing detailed description is provided for clarity only and is merely exemplary. The spirit and scope of the present invention are not limited to the above examples, but are encompassed by the following claims. The contents of all references cited herein are incorporated by reference in their entireties.

Sequence CWU 1

1

315115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 1cggaacatct tcttc 15215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 2tcagaagatg cggaa 15315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 3cctgataagc gtgag 15415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 4gagtccactt aggcc 15515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 5cacttaggcc cacca 15615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 6aggcccacca ttatt 15715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 7aacggacgag aagct 15815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 8tcctttgaca actct 15915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 9aacggacgag aagct 151015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 10agagatagag ccttc 151115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 11tttgacaact ctaga 151215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 12cttctctgat gttag 151315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 13ggataatatt ttgaa 151415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 14ttcaaaagtg aaaga 151515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 15agacggtctt gctgt 151615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 16atccgcgctg catta 151715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 17agaacatatg tgtaa 151815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 18taacctttta ggagc 151915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 19acaaggacgt tagta 152015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 20ctttcctccc gagtg 152115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 21cctacccatc agagg 152215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 22ggacgttagt aactg 152315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 23cttgctccac cggaa 152415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 24ctttttccac cggag 152515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 25atggttttga tttga 152615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 26ctttttccac cggag 152715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 27cgtataacaa tcgaa 152815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 28cgtataacaa tcaaa 152915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 29aacaggaaga agctt 153015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 30ggagtaaagt taata 153115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 31atacctttgc tcatt 153215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 32catctgatac tggca 153315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 33ttgcttcttt gctga 153415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 34agcttgagtc tcgta 153515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 35gctcctctga cgtta 153615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 36ataatatatt gaacc 153715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 37ttcaatagtg aaaga 153815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 38aactatgcac gtctt 153915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 39gagcggatga aggga 154015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 40cttgctcctg gattc 154115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 41aatctgcctg gtagt 154215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 42ataacgtccg gaaac 154315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 43ccgcatacgt cctga 154415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 44agatgagcct aggtc 154515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 45gacgatccgt aactg 154615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 46cagtaagtta atacc 154715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 47caacagaata agcac 154815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 48tccaaaacta ctgag 154915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 49ctgagctaga gtacg 155015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 50aatttcctgt gtagc 155115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 51gcgtagatat aggaa 155215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 52accacctgga ctgat 155315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 53tgtcgactag ccgtt 155415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 54agccgttggg atcct 155515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 55tgagatctta gtggc 155615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 56aactcagaca caggt 155715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 57ttgtccttag ttacc 155815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 58ctgaaggagg agctt 155915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 59aggagcttgc ttctc 156015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 60atgacatttg cttaa 156115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 61acttgcatca ctacc 156215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 62aatggacgga agtct 156315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 63aagaacgagt gtgag 156415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 64aaagttcaca ctgtg 156515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 65tatcttacca gaaag 156615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 66ttagataagt ctgaa 156715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 67aaaggctgtg gctta 156815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 68ttaaccatag taggc 156915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 69aaactgttta acttg 157015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 70acttgagtgc aagag 157115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 71tctctggctt gtaac 157215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 72cctctgaccg ctcta 157315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 73agaactggtg cttgc 157415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 74ctggtgcttg caccg 157515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 75ttgcaccggt tcaag 157615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 76taacctacct catag 157715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 77ataagagaga ctaac 157815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 78agactaacgc atgtt 157915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 79agtaatttaa aaggg 158015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 80aattgctcca ctatg 158115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 81ctccactatg agatg 158215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 82ttagagaaga atgat 158315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 83gaaaatccac caagt 158415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 84tgacggtaac taacc 158515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 85aaaggcattg gctca 158615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 86ctcaaccaat gtacg 158715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 87aaactggaga acttg 158815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 88cttgagtgca gaagg 158915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 89gcttagtgcc ggagc 159015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 90atagagtttt acttc 159115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 91ggtacatcgg tgaca 159215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 92aaggcgataa ggtta 159315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 93tgccagcggt taggc 159415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 94aaggcgatga ggtta 159515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 95aaggcgttaa ggtta 159615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 96ataaccttgg cgatt 159715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 97tgccagcggt ccggc 159815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 98aacaggagaa agctt 159915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 99tttcttgctg acgag 1510015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 100ggatctgccc gatag 1510115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 101ataatgtcta cggac 1510215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 102tacggaccaa agcag 1510315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 103ttgcactatc ggatg 1510415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 104cggatgaacc catat 1510515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 105aatacccttg tcaat 1510615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 106tcaattaagt cagat 1510715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 107atctgaaact ggttg 1510815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 108atttagaggt tgtgg 1510915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 109ttgtggtctt gaacc 1511015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 110agcgaatcct ttaga 1511115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 111gactgggaca acttc 1511215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 112ataatatgtt gaacc 1511315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 113gtcttaggat cgtaa 1511415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 114ggataacata ttgaa

1511515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 115aaaggcggct ttgct 1511615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 116tctgttatca gggaa 1511715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 117gtacctgatc agaaa 1511815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 118agatggcttt gctat 1511915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 119gagatagaag tttcc 1512015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 120aaggtggtga gctta 1512115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 121ttaatacgtt catca 1512215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 122aaggtagctt gctac 1512315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 123ttgctaccgg accta 1512415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 124aatgcttagg aatct 1512515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 125aatctgccta ttagt 1512615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 126acaacatctc gaaag 1512715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 127aaagggatgc taata 1512815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 128accttgcgct aatag 1512915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 129atgagcctaa gtcgg 1513015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 130cgatctgtag cgggt 1513115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 131aaccctgatc cagcc 1513215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 132aggctacttt agtta 1513315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 133tttagttaat accta 1513415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 134tacctagaga tagtg 1513515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 135atagtggacg ttact 1513615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 136cagccatctg gccta 1513715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 137gcctaatact gacgc 1513815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 138tctactagcc gttgg 1513915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 139cctttgaggc tttag 1514015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 140cgataagtag accgc 1514115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 141gtcgcaagac taaaa 1514215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 142tggccttgac atact 1514315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 143atactagaaa ctttc 1514415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 144aatctagata caggt 1514515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 145ttttccttac ttgcc 1514615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 146ccagcatttc ggatg 1514715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 147actttaagga tactg 1514815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 148ttgctacaca gcgat 1514915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 149atgtgatgct aatct 1515015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 150taatctcaaa aagcc 1515115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 151aagccgatcg tagtc 1515215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 152aatgccgcgg tgaat 1515315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 153tagcctaact gcaaa 1515415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 154aacggcagca cagta 1515515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 155taagagcttg ctctt 1515615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 156gaatacatcg gaatc 1515715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 157aaacttacgc taata 1515815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 158atccagctgg ttaat 1515915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 159gtacccaaag aataa 1516015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 160ttgtttaagt ctgtt 1516115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 161agctacctgg accaa 1516215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 162tgcaatttgg cacgc 1516315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 163aacgcgttaa gttcg 1516415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 164ctgcaagccg gcgac 1516515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 165agaaacccta tctca 1516615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 166agcattgctg cggtg 1516715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 167aacagataag gagct 1516815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 168aacagacgag gagct 1516915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 169aacagacaag gagct 1517015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 170ctcctttgac gttag 1517115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 171ctcctctgac gttag 1517215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 172ctcttttgac gttag 1517315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 173cttctctgac gttag 1517415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 174ggataatatt tcgaa 1517515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 175ggataacata ttgaa 1517615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 176ggataatata ttgaa 1517715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 177ggataatatg ttgaa 1517815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 178agatggtttt gctat 1517915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 179aggcggcttt gctgt 1518015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 180agacggtttt gctgt 1518115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 181agatggcttt gctat 1518215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 182acccgcgccg tatta 1518315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 183acctgcgccg tatta 1518415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 184atccgcgccg tatta 1518515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 185atccgcgccg catta 1518615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 186agaacatacg tgtaa 1518715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 187agaacaaacg tgtaa 1518815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 188agaacaaatg tgtaa 1518915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 189taaccatttg gagct 1519015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 190taaccattta tggag 1519115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 191aatgtcttcg gactc 1519215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 192catctttctg atgcg 1519315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 193ggctagcctt ttggc 1519415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 194atcttttttg atgcg 1519515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 195tggctagcct ttttg 1519615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 196tattcagtag tcaga 1519715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 197ctttactaca tggta 1519815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 198atggtataac tgtgg 1519915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 199atgcttaaaa tctcg 1520015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 200tccgattttt tcgtg 1520115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 201tgtactggaa tgcac 1520215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 202aaccccaagt ccttg 1520315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 203ttgtggcttg gcggc 1520415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 204acgtttggtt ctatt 1520515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 205tattcaattg tcaga 1520615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 206tgttttttta gtgac 1520715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 207taaatagtgg tgcta 1520815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 208atttgctggt tgtcc 1520915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 209tatcggtttc aagcc 1521015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 210agcgagtcta acctt 1521115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 211tcttggtaat cttgt 1521215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 212ttctgggtag ccatt 1521315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 213gccatttatg gcgaa 1521415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 214ggtagccatt tatgg 1521515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 215ctatcgactt caagt 1521615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 216taccttacta catgg 1521715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 217actacatgga tacct 1521815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 218tgctaaaaac ctcga 1521915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 219taaaaaacca atgcc 1522015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 220taacgaatcg catgg 1522115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 221taccatggtg gcaac 1522215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 222aatctaaatc cctta 1522315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 223agtactggtc cggct 1522415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 224gaacctcatg gcctt 1522515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 225gccttcactg gctgt 1522615DNAArtificial SequenceDescription of Artificial Sequence

Synthetic probe 226tttctatgat gaccc 1522715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 227tcggccctta aatag 1522815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 228gagtacatca ccttg 1522915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 229cttgttaaac cctgt 1523015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 230agctcgtgcc gatta 1523115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 231ggactaccag ggtat 1523215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 232accagggtat ctaat 1523315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 233cgggaacgta ttcac 1523415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 234cagcagccgc ggtaa 1523515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 235atgtggttta attcg 1523614DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 236gtgccagcag ccgc 1423714DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 237aacgagcgca accc 1423814DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 238gtggtttaat tcga 1423915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 239accttgttac gactt 1524015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 240cgacagagtt tgatc 1524115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 241accttgttac gactt 1524215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 242cagccgcggt aatac 1524315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 243aacaggatta gatac 1524415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 244gtcgtcagct cgtgt 1524515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 245atgttgggtt aagtc 1524615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 246gaatcgctag taatc 1524715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 247cttgtacaca ccgcc 1524820DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 248ggactaccag ggtatctaat 2024915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 249gtgaaactgc gaatg 1525015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 250ctgcgaatgg ctcat 1525115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 251gctcattaaa tcagt 1525215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 252tcagttatcg tttat 1525315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 253cgtttatttg atagt 1525415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 254tctagagcta ataca 1525515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 255tagagctaat acatg 1525615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 256tgtatttatt agata 1525715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 257ttattagata aaaaa 1525815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 258tggttcattc aaatt 1525915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 259ttcaaatttc tgccc 1526015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 260ctgccctatc aactt 1526115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 261aactttcgat ggtag 1526215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 262tcgatggtag gatag 1526315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 263agggttcgat tccgg 1526415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 264agcctgagaa acggc 1526515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 265agaaacggct accac 1526615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 266ctaccacatc caagg 1526715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 267atccaaggaa ggcag 1526815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 268aaggcagcag gcgcg 1526915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 269aggcgcgcaa attac 1527015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 270caaattaccc aatcc 1527115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 271gaggtagtga caata 1527215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 272gtaattggaa tgagt 1527315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 273tggaatgagt acaat 1527415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 274ccttaacgag gaaca 1527515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 275caagtctggt gccag 1527615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 276taattccagc tccaa 1527715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 277agcgtatatt aaagt 1527815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 278ttaaagttgt tgcag 1527915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 279gttgcagtta aaaag 1528015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 280agctcgtagt tgaac 1528115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 281aaattagagt gttca 1528215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 282gtgttcaaag caggc 1528316DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 283attagcatgg aataat 1628415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 284ggttctattt tgttg 1528515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 285tgttggtttc tagga 1528615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 286gtcagaggtg aaatt 1528715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 287tgaaattctt ggatt 1528815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 288tgaagactaa ctact 1528915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 289tactgcgaaa gcatt 1529014DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 290gttttcatta atca 1429114DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 291aacgaaagtt aggg 1429215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 292gatcagatac cgtcg 1529315DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 293accgtcgtag tctta 1529415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 294tagtcttaac cataa 1529515DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 295accataaact atgcc 1529616DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 296tatgccgact agggat 1629715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 297tcggcacctt acgag 1529815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 298tacgagaaat caaag 1529915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 299agtatggtcg caagg 1530015DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 300ggctgaaact taaag 1530115DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 301agcctgcggc ttaat 1530215DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 302ttaatttgac tcaac 1530316DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 303aaactcacca ggtcca 1630415DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 304tggagtgatt tgtct 1530518DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 305tgtctgctta attgcgat 1830615DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 306aacaggtctg tgatg 1530715DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 307tgtgatgccc ttaga 1530815DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 308gcgcgctaca ctgac 1530915DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 309ttgctcttca acgag 1531020DNAArtificial SequenceDescription of Artificial Sequence Synthetic primer 310agagtttgat cctggctcag 2031123DNAArtificial SequenceDescription of Artificial Sequence Synthetic primer 311atcggctacc ttgttacgac ttc 2331226DNAArtificial SequenceDescription of Artificial Sequence Synthetic primer 312aaatcagtta tcgtttattt gatagt 2631319DNAArtificial SequenceDescription of Artificial Sequence Synthetic primer 313attcctcgtt gaagagcaa 1931422DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 314gcgaacggac gagaagcttg ct 2231522DNAArtificial SequenceDescription of Artificial Sequence Synthetic probe 315gcgaacagac gaggagcttg ct 22

Claims

1.-39. (canceled)

40. A composition comprising at least one .gamma.PNA probe having a sequence that is 1 or 2 nucleobases shorter at either one or both ends of at least one sequence selected from the group consisting of: SEQ ID NOS: 1-309, any reverse, reverse complementary, or complementary sequence thereof, and any functional equivalent thereof.

41. The composition of claim 40, further comprising a support substrate.

42. The composition of claim 41, wherein the support substrate is selected from the group consisting of: a magnetic bead, a well, a plate, a test tube, a stick, a plastic slide, a glass slide, and a biochip.

43. The composition of claim 42, wherein the support substrate is coated with Avidin Neutravidin, or Streptavidin.

44. The composition of claim 40, wherein the at least one .gamma.PNA probe comprises one or more functional moiety selected from the group consisting of: a binding molecule, a spacer group, a linker group, a hydrophobicity-changing group, a charge-inducing group, and a structural change-inducing group.

45. The composition of claim 40, wherein the at least one .gamma.PNA probe emits a detectable signal selected from the group consisting of: fluorescence, luminescence, FRET, colorimetric, calorimetric, interference patterns, pH, resistance/conductivity, enzymatic function and kinetics, protein structure, and electrical potential.

46. The composition of claim 40, wherein the at least one .gamma.PNA probe comprises at least one poly(ethylene)glycol linker.

47. The composition of claim 46, wherein the at least one poly(ethylene)glycol linker comprises of one or more biotinylation sites.

48. The composition of claim 40, wherein the .gamma.PNA probe comprises a carbon linker or spacer including one or more biotinylation sites, and an amino acid-based side chain.

49. The composition of claim 48, wherein the carbon linker or spacer comprises 1-100 carbons.

50. The composition of claim 48, wherein the carbon linker or spacer comprises poly(ethylene)glycol.

51. A kit comprising the composition of claim 40.

52. A method for diagnosing an infection in a subject comprising contacting genomic material from a clinical sample obtained from the subject with the composition of claim 40 to form a mixture; heating the mixture; invading a plurality of targeted infection-related genomic material with the at least one .gamma.PNA probe of the composition; and detecting the presence of one or more targeted genomic material that is indicative of an infection.

53. A .gamma.PNA probe comprising (a) a peptide backbone, (b) a nucleic acid sequence corresponding to a target gene from a bacterial or fungal pathogen, (c) a poly(ethylene)glycol linker including more than one biotinylation site, and (d) an amino acid-based side chain along the peptide backbone of .gamma.PNA probe.

54. A method for diagnosing an infection in a subject comprising contacting genomic material from a clinical sample obtained from the subject with the .gamma.PNA probe of claim 53 to form a mixture; heating the mixture; invading a plurality of targeted infection-related genomic material with the .gamma.PNA probe; and detecting the presence of one or more targeted genomic material that is indicative of an infection.