TERMINATOR SEQUENCE FOR GENE EXPRESSION IN PLANTS

Abstract

The present invention discloses polynucleotide sequences that can be used to regulate gene expression in plants. Terminator sequences from Sorghum bicolor that are functional in plants are disclosed.

Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit of U.S. Provisional Application No. 61/514,055, filed Aug. 2, 2011, the entire content of which is herein incorporated by reference.

FIELD OF INVENTION

[0002] The present invention relates to the field of plant molecular biology and plant genetic engineering. More specifically, it relates to novel plant terminator sequences and their use to regulate gene expression in plants.

BACKGROUND

[0003] Recent advances in plant genetic engineering have opened new doors to engineer plants to have improved characteristics or traits. These transgenic plants characteristically have recombinant DNA constructs in their genome that have protein coding region operably linked to multiple regulatory regions that allow accurate expression of the transgene. A few examples of regulatory elements that help regulate gene expression in transgenic plants are promoters, introns, terminators, enhancers and silencers.

[0004] Plant genetic engineering has advanced to introducing multiple traits into commercially important plants, also known as gene stacking. This is accomplished by multigene transformation, where multiple genes are transferred to create a transgenic plant that might express a complex phenotype, or multiple phenotypes. But it is important to modulate or control the expression of each transgene optimally. The regulatory elements need to be diverse, to avoid introducing into the same transgenic plant repetitive sequences, which has been correlated with undesirable negative effects on transgene expression and stability (Peremarti et al (2010) Plant Mol Biol 73:363-378; Mette et al (1999) EMBO J 18:241-248; Mette et al (2000) EMBO J 19:5194-5201; Mourrain et al (2007) Planta 225:365-379, U.S. Pat. Nos. 7,632,982, 7,491,813, 7,674,950, PCT Application No. PCT/US2009/046968). Therefore it is important to discover and characterize novel regulatory elements that can be used to express heterologous nucleic acids in important crop species. Diverse regulatory regions can be used to control the expression of each transgene optimally.

[0005] Regulatory sequences located downstream of coding regions contain signals required for transcription termination and 3' mRNA processing, and are called terminator sequences. The terminator sequences play a key role in mRNA processing, localization, stability and translation (Proudfoot, N. (2004) Curr. Op. Cell Biol 16:272-278.; Gilmartin, 2005). The 3' regulatory sequences contained in terminator sequences can affect the level of expression of a gene. Optimal expression of a chimeric gene in plant cells has been found to be dependent on the presence of appropriate 3' sequences (Ingelbrecht, I. L. W. et al (1989) Plant Cell 1:671-680). Read through transcription through leaky terminator of a gene can cause unwanted transcription of one transgene from promoter of another one. Also, bidirectional, convergent transcription of transgenes in transgenic plants can occur due to leaky transcription termination of separate convergent genes or from genomic promoters. Convergent, overlapping transcription can decrease transgene expression, or generate antisense RNA (Bieri, S. et al (2002) Molecular Breeding 10:107-117). This underlines the importance of discovering novel and efficient transcriptional terminators.

SUMMARY

[0006] The present invention relates to regulatory sequences for modulating gene expression in plants. Specifically, the present invention relates to terminator sequences. Recombinant DNA constructs comprising terminator sequences are provided.

[0007] An embodiment of this invention is an isolated polynucleotide sequence comprising: (a) the sequence set forth in SEQ ID NO:1 or SEQ ID NO:18; (b) a sequence with at least 95% sequence identity to SEQ ID NO:1 or SEQ ID NO:18; or (c) a sequence comprising a functional fragment of (a) or (b), wherein the isolated polynucleotide sequence functions as a terminator in a plant cell. Another embodiment of this invention is a recombinant construct comprising an isolated polynucleotide sequence comprising: (a) the sequence set forth in SEQ ID NO:1 or SEQ ID NO:18; (b) a sequence with at least 95% sequence identity to SEQ ID NO:1 or SEQ ID NO:18; or (c) a sequence comprising a functional fragment of (a) or (b), wherein the isolated polynucleotide sequence functions as a terminator in a plant cell. This recombinant construct may further comprise a promoter and a heterologous polynucleotide, wherein the promoter and the heterologous polynucleotide are operably linked to the isolated polynucleotide sequence.

[0008] Another embodiment of this invention is a method of expressing a heterologous polynucleotide in a plant, comprising the steps of (a) introducing into a regenerable plant cell the recombinant DNA construct described above; (b) regenerating a transgenic plant from the regenerable plant cell of (a); and (c) obtaining a progeny plant from the transgenic plant of step (b), wherein the transgenic plant and the progeny plant comprises the recombinant DNA construct and exhibits expression of the heterologous polynucleotide.

[0009] In another embodiment, this invention concerns a vector, virus, cell, microorganism, plant, or seed comprising a recombinant DNA construct comprising the terminator sequences described in the present invention.

[0010] The invention encompasses regenerated, mature and fertile transgenic plants comprising the recombinant DNA constructs described above, transgenic seeds produced therefrom, T1 and subsequent generations. The transgenic plant cells, tissues, plants, and seeds may comprise at least one recombinant DNA construct of interest.

[0011] In another embodiment, the plant or seed comprising the terminator sequences described in the present invention is a monocotyledenous plant or seed. In another embodiment, the plant or seed comprising the terminator sequences described in the present invention is a maize plant or seed.

[0012] In another embodiment, any of the methods of expressing a heterologous polynucleotide, wherein the plant cell is a monocotyledonous plant cell, e.g., a maize plant cell.

BRIEF DESCRIPTION OF DRAWINGS AND SEQUENCE LISTING

[0013] The invention can be more fully understood from the following detailed description and the accompanying drawings and Sequence Listing which form a part of this application. The Sequence Listing contains the one letter code for nucleotide sequence characters and the three letter codes for amino acids as defined in conformity with the IUPAC-IUBMB standards described in Nucleic Acids Research 13:3021-3030 (1985) and in the Biochemical Journal 219 (No. 2): 345-373 (1984), which are herein incorporated by reference in their entirety. The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. .sctn. 1.822.

[0014] FIG. 1 shows the map of PHP31801, the vector used for cloning SB-GKAF terminator after amplification.

[0015] FIG. 2 shows the map of PHP34074, the vector used for testing the SB-GKAF terminator.

[0016] FIG. 3 shows the results of testing SB-GKAF terminator compared to PINII terminator in transient assays. It shows quantitative analysis of GUS reporter gene expression in BMS cells transformed with PHP34074 (SB-GKAF terminator) and PHP34005 (PINII terminator).

[0017] FIG. 4A and FIG. 4B show quantitative analysis of GUS reporter gene expression in Gaspe Flint derived maize lines stably transformed with SB-GKAF (PHP34074) and PINII (PHP34005) terminator constructs. FIG. 4A shows GUS reporter gene expression assayed at protein level, and FIG. 4B shows GUS reporter gene expression assayed with qRT-PCR.

[0018] FIG. 5 shows the results of qRT-PCR assays with stably transformed Gaspe Flint derived maize lines, using two sets of primers downstream of the SB-GKAF terminator and the PINII terminator.

[0019] FIG. 6A-6C show the alignment between the cloned SB-GKAF terminator (SEQ ID NO:1) and the nucleotides 1863 to 2322 of NCBI GI NO: 671655 (SEQ ID NO:18). The consensus sequence is show at the top, and the residues that match the consensus exactly are boxed.

[0020] SEQ ID NO:1 is the sequence of the 459 bp SB-GKAF terminator.

[0021] SEQ ID NO:2 and 3 are the sequences of the forward and reverse primers used to amplify SB-GKAF terminator.

[0022] SEQ ID NO:4 is the nucleotide sequence of PHP31801, the vector used for cloning SB-GKAF terminator after PCR amplification.

[0023] SEQ ID NO:5 is the nucleotide sequence of PHP34074, the vector used for testing SB-GKAF terminator.

[0024] SEQ ID NO:6 is the nucleotide sequence of PHP34005, the test vector used as a control with PINII terminator.

[0025] SEQ ID NOS:7-9 are the sequences of the forward primer, reverse primer and probe used for assessing GUS expression by qRT-PCR in transgenic maize plants, as described in Table 2.

[0026] SEQ ID NOS:10-17 are the sequences of the primers used for quantitating read through transcription through SB-GKAF and PINII terminators, by qRT-PCR in transgenic maize plants, as described in Table 3.

[0027] SEQ ID NO:18 corresponds to nucleotides 1863 to 2322 of NCBI GI NO: 671655.

DETAILED DESCRIPTION

[0028] The disclosure of each reference set forth herein is hereby incorporated by reference in its entirety.

[0029] As used herein and in the appended claims, the singular forms "a", "an", and "the" include plural reference unless the context clearly dictates otherwise. Thus, for example, reference to "a plant" includes a plurality of such plants, reference to "a cell" includes one or more cells and equivalents thereof known to those skilled in the art, and so forth.

[0030] As used herein:

[0031] The terms "monocot" and "monocotyledonous plant" are used interchangeably herein. A monocot of the current invention includes the Gramineae.

[0032] The terms "dicot" and "dicotyledonous plant" are used interchangeably herein. A dicot of the current invention includes the following families: Brassicaceae, Leguminosae, and Solanaceae.

[0033] The terms "full complement" and "full-length complement" are used interchangeably herein, and refer to a complement of a given nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.

[0034] "Transgenic" refers to any cell, cell line, callus, tissue, plant part or plant, the genome of which has been altered by the presence of a heterologous nucleic acid, such as a recombinant DNA construct, including those initial transgenic events as well as those created by sexual crosses or asexual propagation from the initial transgenic event. The term "transgenic" as used herein does not encompass the alteration of the genome (chromosomal or extra-chromosomal) by conventional plant breeding methods or by naturally occurring events such as random cross-fertilization, non-recombinant viral infection, non-recombinant bacterial transformation, non-recombinant transposition, or spontaneous mutation.

[0035] "Genome" as it applies to plant cells encompasses not only chromosomal DNA found within the nucleus, but organelle DNA found within subcellular components (e.g., mitochondrial, plastid) of the cell.

[0036] "Plant" includes reference to whole plants, plant organs, plant tissues, plant propagules, seeds and plant cells and progeny of same. Plant cells include, without limitation, cells from seeds, suspension cultures, embryos, meristematic regions, callus tissue, leaves, roots, shoots, gametophytes, sporophytes, pollen, and microspores.

[0037] "Propagule" includes all products of meiosis and mitosis able to propagate a new plant, including but not limited to, seeds, spores and parts of a plant that serve as a means of vegetative reproduction, such as corms, tubers, offsets, or runners. Propagule also includes grafts where one portion of a plant is grafted to another portion of a different plant (even one of a different species) to create a living organism. Propagule also includes all plants and seeds produced by cloning or by bringing together meiotic products, or allowing meiotic products to come together to form an embryo or fertilized egg (naturally or with human intervention).

[0038] "Progeny" comprises any subsequent generation of a plant.

[0039] "Transgenic plant" includes reference to a plant which comprises within its genome a heterologous polynucleotide. For example, the heterologous polynucleotide is stably integrated within the genome such that the polynucleotide is passed on to successive generations. The heterologous polynucleotide may be integrated into the genome alone or as part of a recombinant DNA construct.

[0040] The commercial development of genetically improved germplasm has also advanced to the stage of introducing multiple traits into crop plants, often referred to as a gene stacking approach. In this approach, multiple genes conferring different characteristics of interest can be introduced into a plant. Gene stacking can be accomplished by many means including but not limited to co-transformation, retransformation, and crossing lines with different transgenes.

[0041] "Transgenic plant" also includes reference to plants which comprise more than one heterologous polynucleotide within their genome. Each heterologous polynucleotide may confer a different trait to the transgenic plant.

[0042] "Heterologous" with respect to sequence means a sequence that originates from a foreign species, or, if from the same species, is substantially modified from its native form in composition and/or genomic locus by deliberate human intervention. "Polynucleotide", "nucleic acid sequence", "nucleotide sequence", or "nucleic acid fragment" are used interchangeably to refer to a polymer of RNA or DNA that is single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases. Nucleotides (usually found in their 5'-monophosphate form) are referred to by their single letter designation as follows: "A" for adenylate or deoxyadenylate (for RNA or DNA, respectively), "C" for cytidylate or deoxycytidylate, "G" for guanylate or deoxyguanylate, "U" for uridylate, "T" for deoxythymidylate, "R" for purines (A or G), "Y" for pyrimidines (C or T), "K" for G or T, "H" for A or C or T, "I" for inosine, and "N" for any nucleotide.

[0043] "Polypeptide", "peptide", "amino acid sequence" and "protein" are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical analogue of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers. The terms "polypeptide", "peptide", "amino acid sequence", and "protein" are also inclusive of modifications including, but not limited to, glycosylation, lipid attachment, sulfation, gamma-carboxylation of glutamic acid residues, hydroxylation and ADP-ribosylation.

[0044] "Messenger RNA (mRNA)" refers to the RNA that is without introns and that can be translated into protein by the cell.

[0045] "cDNA" refers to a DNA that is complementary to and synthesized from an mRNA template using the enzyme reverse transcriptase. The cDNA can be single-stranded or converted into the double-stranded form using the Klenow fragment of DNA polymerase I.

[0046] "Coding region" refers to the portion of a messenger RNA (or the corresponding portion of another nucleic acid molecule such as a DNA molecule) which encodes a protein or polypeptide. "Non-coding region" refers to all portions of a messenger RNA or other nucleic acid molecule that are not a coding region, including but not limited to, for example, the promoter region, 5' untranslated region ("UTR"), 3' UTR, intron and terminator. The terms "coding region" and "coding sequence" are used interchangeably herein. The terms "non-coding region" and "non-coding sequence" are used interchangeably herein.

[0047] An "Expressed Sequence Tag" ("EST") is a DNA sequence derived from a cDNA library and therefore is a sequence which has been transcribed. An EST is typically obtained by a single sequencing pass of a cDNA insert. The sequence of an entire cDNA insert is termed the "Full-Insert Sequence" ("FIS"). A "Contig" sequence is a sequence assembled from two or more sequences that can be selected from, but not limited to, the group consisting of an EST, FIS and PCR sequence. A sequence encoding an entire or functional protein is termed a "Complete Gene Sequence" ("CGS") and can be derived from an FIS or a contig.

[0048] "Mature" protein refers to a post-translationally processed polypeptide; i.e., one from which any pre- or pro-peptides present in the primary translation product have been removed.

[0049] "Precursor" protein refers to the primary product of translation of mRNA; i.e., with pre- and pro-peptides still present. Pre- and pro-peptides may be and are not limited to intracellular localization signals.

[0050] "Isolated" refers to materials, such as nucleic acid molecules and/or proteins, which are substantially free or otherwise removed from components that normally accompany or interact with the materials in a naturally occurring environment. Isolated polynucleotides may be purified from a host cell in which they naturally occur. Conventional nucleic acid purification methods known to skilled artisans may be used to obtain isolated polynucleotides. The term also embraces recombinant polynucleotides and chemically synthesized polynucleotides.

[0051] "Recombinant" refers to an artificial combination of two otherwise separated segments of sequence, e.g., by chemical synthesis or by the manipulation of isolated segments of nucleic acids by genetic engineering techniques.

[0052] "Recombinant" also includes reference to a cell or vector, that has been modified by the introduction of a heterologous nucleic acid or a cell derived from a cell so modified, but does not encompass the alteration of the cell or vector by naturally occurring events (e.g., spontaneous mutation, natural transformation/transduction/transposition) such as those occurring without deliberate human intervention.

[0053] "Recombinant DNA construct" refers to a combination of nucleic acid fragments that are not normally found together in nature. Accordingly, a recombinant DNA construct may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that normally found in nature. The terms "recombinant DNA construct" and "recombinant construct" are used interchangeably herein.

[0054] The terms "entry clone" and "entry vector" are used interchangeably herein.

[0055] "Regulatory sequences" or "regulatory elements" are used interchangeably and refer to nucleotide sequences located upstream (5' non-coding sequences), within, or downstream (3' non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence. Regulatory sequences may include, but are not limited to, promoters, translation leader sequences, introns, and polyadenylation recognition sequences. The terms "regulatory sequence" and "regulatory element" are used interchangeably herein.

[0056] "Promoter" refers to a nucleic acid fragment capable of controlling transcription of another nucleic acid fragment.

[0057] "Promoter functional in a plant" is a promoter capable of controlling transcription in plant cells whether or not its origin is from a plant cell.

[0058] "Tissue-specific promoter" and "tissue-preferred promoter" are used interchangeably to refer to a promoter that is expressed predominantly but not necessarily exclusively in one tissue or organ, but that may also be expressed in one specific cell.

[0059] "Developmentally regulated promoter" refers to a promoter whose activity is determined by developmental events.

[0060] Promoters that cause a gene to be expressed in most cell types at most times are commonly referred to as "constitutive promoters".

[0061] Inducible promoters selectively express an operably linked DNA sequence in response to the presence of an endogenous or exogenous stimulus, for example by chemical compounds (chemical inducers) or in response to environmental, hormonal, chemical, and/or developmental signals. Examples of inducible or regulated promoters include, but are not limited to, promoters regulated by light, heat, stress, flooding or drought, pathogens, phytohormones, wounding, or chemicals such as ethanol, jasmonate, salicylic acid, or safeners.

[0062] "Enhancer sequences" refer to the sequences that can increase gene expression. These sequences can be located upstream, within introns or downstream of the transcribed region. The transcribed region is comprised of the exons and the intervening introns, from the promoter to the transcription termination region. The enhancement of gene expression can be through various mechanisms which include, but are not limited to, increasing transcriptional efficiency, stabilization of mature mRNA and translational enhancement.

[0063] An "intron" is an intervening sequence in a gene that is transcribed into RNA and then excised in the process of generating the mature mRNA. The term is also used for the excised RNA sequences. An "exon" is a portion of the sequence of a gene that is transcribed and is found in the mature messenger RNA derived from the gene, and is not necessarily a part of the sequence that encodes the final gene product.

[0064] "Operably linked" refers to the association of nucleic acid fragments in a single fragment so that the function of one is regulated by the other. For example, a promoter is operably linked with a nucleic acid fragment when it is capable of regulating the transcription of that nucleic acid fragment.

[0065] "Expression" refers to the production of a functional product. For example, expression of a nucleic acid fragment may refer to transcription of the nucleic acid fragment (e.g., transcription resulting in mRNA or functional RNA) and/or translation of mRNA into a precursor or mature protein.

[0066] "Overexpression" refers to the production of a gene product in transgenic organisms that exceeds levels of production in a null segregating (or non-transgenic) organism from the same experiment.

[0067] "Phenotype" means the detectable characteristics of a cell or organism.

[0068] The term "crossed" or "cross" means the fusion of gametes via pollination to produce progeny (e.g., cells, seeds or plants). The term encompasses both sexual crosses (the pollination of one plant by another) and selfing (self-pollination, e.g., when the pollen and ovule are from the same plant). The term "crossing" refers to the act of fusing gametes via pollination to produce progeny.

[0069] A "favorable allele" is the allele at a particular locus that confers, or contributes to, a desirable phenotype, e.g., increased cell wall digestibility, or alternatively, is an allele that allows the identification of plants with decreased cell wall digestibility that can be removed from a breeding program or planting ("counterselection"). A favorable allele of a marker is a marker allele that segregates with the favorable phenotype, or alternatively, segregates with the unfavorable plant phenotype, therefore providing the benefit of identifying plants.

[0070] The term "introduced" means providing a nucleic acid (e.g., expression construct) or protein into a cell. Introduced includes reference to the incorporation of a nucleic acid into a eukaryotic or prokaryotic cell where the nucleic acid may be incorporated into the genome of the cell, and includes reference to the transient provision of a nucleic acid or protein to the cell. Introduced includes reference to stable or transient transformation methods, as well as sexually crossing. Thus, "introduced" in the context of inserting a nucleic acid fragment (e.g., a recombinant DNA construct/expression construct) into a cell, means "transfection" or "transformation" or "transduction" and includes reference to the incorporation of a nucleic acid fragment into a eukaryotic or prokaryotic cell where the nucleic acid fragment may be incorporated into the genome of the cell (e.g., chromosome, plasmid, plastid or mitochondrial DNA), converted into an autonomous replicon, or transiently expressed (e.g., transfected mRNA).

[0071] "Suppression DNA construct" is a recombinant DNA construct which when transformed or stably integrated into the genome of the plant, results in "silencing" of a target gene in the plant. The target gene may be endogenous or transgenic to the plant. "Silencing," as used herein with respect to the target gene, refers generally to the suppression of levels of mRNA or protein/enzyme expressed by the target gene, and/or the level of the enzyme activity or protein functionality. The terms "suppression", "suppressing" and "silencing", used interchangeably herein, include lowering, reducing, declining, decreasing, inhibiting, eliminating or preventing. "Silencing" or "gene silencing" does not specify mechanism and is inclusive, and not limited to, anti-sense, cosuppression, viral-suppression, hairpin suppression, stem-loop suppression, RNAi-based approaches, and small RNA-based approaches.

[0072] Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described more fully in Sambrook, J., Fritsch, E. F. and Maniatis, T. Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, 1989 (hereinafter "Sambrook").

[0073] "Transcription terminator", "termination sequences", or "terminator" refer to DNA sequences located downstream of a coding sequence, including polyadenylation recognition sequences and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3' end of the mRNA precursor. The use of different 3' non-coding sequences is exemplified by Ingelbrecht, I. L., et al., Plant Cell 1:671-680 (1989). A polynucleotide sequence with "terminator activity" refers to a polynucleotide sequence that, when operably linked to the 3' end of a second polynucleotide sequence that is to be expressed, is capable of terminating transcription from the second polynucleotide sequence. Transcription termination is the process by which RNA synthesis by RNA polymerase is stopped and both the RNA and the enzyme are released from the DNA template.

[0074] Improper termination of an RNA transcript can affect the stability of the RNA, and hence can affect protein expression. Variability of transgene expression is sometimes attributed to variability of termination efficiency (Bieri et al (2002) Molecular Breeding 10: 107-117).

[0075] The terms "SB-GKAF terminator", "GKAF terminator" and "gamma-kafirin terminator" are used interchangeably herein, and each refers to the sequence encoding the 3' untranslated region (3' UTR) of the Sorghum Bicolor gamma-kafirin gene and about 300 bp of sequence downstream from the 3' UTR. The sequence of the SB-GKAF terminator is given in SEQ ID NO:1. The Sorghum bicolor gamma-kafirin gene encodes a gamma-prolamin protein, and the sequence for this gene is given in NCBI GI NO: 671655. Prolam ins are the major storage proteins of many cereals. The Sorghum gamma-Kafirin, which is the .gamma.-prolamin of Sorghum, constitutes about 2-5% of total prolamin in sorghum endosperm, and is composed of a single polypeptide of 27 kDa (de Freitas F A et al (1994) Mol Gen Genetics 245(2):177-86).

[0076] The present invention encompasses functional fragments and variants of the terminator sequences disclosed herein.

[0077] A "functional fragment" of the terminator is defined as any subset of contiguous nucleotides of the terminator sequence disclosed herein, that can perform the same, or substantially similar function as the full length terminator sequence disclosed herein. A "functional fragment" with substantially similar function to the full length terminator disclosed herein refers to a functional fragment that retains the ability to terminate transcription largely at the same level as the full-length terminator sequence. A recombinant construct comprising a heterologous polynucleotide operably linked to a "functional fragment" of the terminator sequence disclosed herein exhibits levels of heterologous polynucleotide expression substantially similar to a corresponding recombinant construct comprising a heterologous polynucleotide operably linked to the full length terminator sequence. A "variant", as used herein, is the sequence of the terminator or the sequence of a functional fragment of a terminator containing changes in which one or more nucleotides of the original sequence is deleted, added, and/or substituted, while substantially maintaining terminator function. One or more base pairs can be inserted, deleted, or substituted internally to a terminator, without affecting its activity. Fragments and variants can be obtained via methods such as site-directed mutagenesis and synthetic construction.

[0078] These terminator functional fragments may comprise at least 50, 75, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425 or 450 contiguous nucleotides of the particular terminator nucleotide sequence disclosed herein. Such fragments may be obtained by use of restriction enzymes to cleave the naturally occurring terminator nucleotide sequences disclosed herein; by synthesizing a nucleotide sequence from the naturally occurring terminator DNA sequence; or may be obtained through the use of PCR technology. See particularly, Mullis et al., Methods Enzymol. 155:335-350 (1987), and Higuchi, R. In PCR Technology: Principles and Applications for DNA Amplifications; Erlich, H. A., Ed.; Stockton Press Inc.: New York, 1989. Again, variants of these terminator fragments, such as those resulting from site-directed mutagenesis, are encompassed by the compositions of the present invention.

[0079] The terms "substantially similar" and "corresponding substantially" as used herein refer to nucleic acid fragments, particularly terminator sequences, wherein changes in one or more nucleotide bases do not substantially alter the ability of the terminator to terminate transcription. These terms also refer to modifications, including deletions and variants, of the nucleic acid sequences of the instant invention by way of deletion or insertion of one or more nucleotides that do not substantially alter the functional properties of the resulting terminator relative to the initial, unmodified terminator. It is therefore understood, as those skilled in the art will appreciate, that the invention encompasses more than the specific exemplary sequences.

[0080] As will be evident to one of skill in the art, any heterologous polynucleotide of interest can be operably linked to the terminator sequences described in the current invention. Examples of polynucleotides of interest that can be operably linked to the terminator sequences described in this invention include, but are not limited to, polynucleotides comprising regulatory elements such as introns, enhancers, promoters, translation leader sequences, protein coding regions such as disease and insect resistance genes, genes conferring nutritional value, genes conferring yield and heterosis increase, genes that confer male and/or female sterility, antifungal, antibacterial or antiviral genes, and the like. Likewise, the terminator sequences described in the current invention can be used to terminate transcription of any nucleic acid that controls gene expression. Examples of nucleic acids that could be used to control gene expression include, but are not limited to, antisense oligonucleotides, suppression DNA constructs, or nucleic acids encoding transcription factors.

[0081] A recombinant DNA construct (including a suppression DNA construct) of the present invention may comprise at least one regulatory sequence. In an embodiment of the present invention, the regulatory sequences disclosed herein can be operably linked to any other regulatory sequence.

[0082] A number of promoters can be used in recombinant DNA constructs of the present invention. The promoters can be selected based on the desired outcome, and may include constitutive, tissue-specific, inducible, or other promoters for expression in the host organism.

[0083] The terms "real-time PCR", "quantitative PCR", "quantitative real-time PCR", and "QPCR" are used interchangeably herein, and represent a variation of the standard polymerase chain reaction (PCR) technique used to quantify DNA or RNA in a sample. Using sequence-specific primers and a probe, the relative number or copies of a particular DNA or RNA sequence are determined. The term relative is used since this technique compares relative copy numbers between different genes with respect to a specific reference gene. The quantification arises by measuring the amount of amplified product at each cycle during the PCR process. Quantification of amplified product is obtained using fluorescent hydrolysis probes that measure increasing fluorescence for each subsequent PCR cycle. The Ct (cycle threshold) is defined as the number of cycles required for the fluorescent signal to cross the threshold (i.e., exceeds background level). DNA/RNA from genes with higher copy numbers will appear after fewer PCR cycles; so the lower a Ct value, the more copies are present in the specific sample. To quantify RNA, QPCR or real-time PCR is preceded by the step of reverse transcribing mRNA into cDNA. This is referred to herein as "real-time RT-PCR" or "quantitative RT-PCR" or "qRT-PCR".

[0084] The Taqman method of PCR product quantification uses a fluorescent reporter probe. This is more accurate since the probe is designed to be sequence-specific and will only bind to the specific PCR product. The probe specificity allows for quantification even in the presence of non-specific DNA amplification. This allows for multiplexing, which quantitates several genes in the same tube, by using probes with different emission spectra. Breakdown of the probe by the 5' to 3' exonuclease activity of Taq polymerase removes the quencher and allows the PCR product to be detected.

[0085] When plotted on a linear scale, the fluorescent emission increase with PCR cycle number has a sigmoidal shape with an exponential phase and a plateau phase. The plateau phase is determined by the amount of primer in the master mix rather than the nucleotide template. Usually the vertical scale is plotted in a logarithmic fashion, allowing the intersection of the plot with the threshold to be linear and more easily visualized. Theoretically, the amount of DNA doubles every cycle during the exponential phase, but this is affected by the efficiency of the primers used. A positive control using a reference gene, e.g., a "housekeeping" gene that is relatively abundant in all cell types, is also performed to allow for comparisons between samples. The amount of DNA/RNA is determined by comparing the results to a standard curve produced by serial dilutions of a known concentration of DNA/RNA.

[0086] The present invention includes a polynucleotide comprising: (i) a nucleic acid sequence of at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity, based on the Clustal V (or Clustal W) method of alignment, when compared to SEQ ID NO:1 or SEQ ID NO:18; or (ii) a nucleic acid sequence of at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence identity, based on the Clustal V (or Clustal W) method of alignment, when compared to a functional fragment of SEQ ID NO:1 or SEQ ID NO:18; or (iii) a full complement of the nucleic acid sequence of (i) or (ii), wherein the polynucleotide acts as a terminator in a plant cell.

[0087] Sequence alignments and percent identity calculations may be determined using a variety of comparison methods designed to detect homologous sequences including, but not limited to, the Megalign.RTM. program of the LASERGENE.RTM. bioinformatics computing suite (DNASTAR.RTM. Inc., Madison, Wis.). Unless stated otherwise, multiple alignment of the sequences provided herein were performed using the Clustal V method of alignment (Higgins and Sharp (1989) CABIOS. 5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments and calculation of percent identity of protein sequences using the Clustal V method are KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5. For nucleic acids these parameters are KTUPLE=2, GAP PENALTY=5, WINDOW=4 and DIAGONALS SAVED=4. After alignment of the sequences, using the Clustal V program, it is possible to obtain "percent identity" and "divergence" values by viewing the "sequence distances" table on the same program; unless stated otherwise, percent identities and divergences provided and claimed herein were calculated in this manner.

[0088] Alternatively, the Clustal W method of alignment may be used. The Clustal W method of alignment (described by Higgins and Sharp, CABIOS. 5:151-153 (1989); Higgins, D. G. et al., Comput. Appl. Biosci. 8:189-191 (1992)) can be found in the MegAlign.TM. v6.1 program of the LASERGENE.RTM. bioinformatics computing suite (DNASTAR.RTM. Inc., Madison, Wis.). Default parameters for multiple alignment correspond to GAP PENALTY=10, GAP LENGTH PENALTY=0.2, Delay Divergent Sequences=30%, DNA Transition Weight=0.5, Protein Weight Matrix=Gonnet Series, DNA Weight Matrix=IUB. For pairwise alignments the default parameters are Alignment=Slow-Accurate, Gap Penalty=10.0, Gap Length=0.10, Protein Weight Matrix=Gonnet 250 and DNA Weight Matrix=IUB. After alignment of the sequences using the Clustal W program, it is possible to obtain "percent identity" and "divergence" values by viewing the "sequence distances" table in the same program.

[0089] Embodiments of the invention include:

[0090] The present invention relates to terminator sequences. Recombinant DNA constructs comprising terminator sequences are provided.

[0091] An embodiment of this invention is an isolated polynucleotide sequence comprising (a) the sequence set forth in SEQ ID NO:1 or SEQ ID NO:18; (b) a sequence with at least 95% sequence identity to SEQ ID NO:1 or SEQ ID NO:18; or (c) a sequence comprising a functional fragment of (a) or (b), wherein the isolated polynucleotide sequence functions as a terminator in a plant cell. In another aspect, this invention concerns a recombinant DNA construct comprising a promoter, at least one heterologous nucleic acid fragment, and any terminator, or combination of terminator elements, of the present invention, wherein the promoter, at least one heterologous nucleic acid fragment, and terminator(s) are operably linked.

[0092] In another embodiment, a functional fragment may comprise at least 450, 425, 400, 375, 350, 325, 300, 275, 250, 225, 200, 175 or 150 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:18.

[0093] Recombinant DNA constructs can be constructed by operably linking the nucleic acid fragment of the invention, the terminator sequences set forth in SEQ ID NO:1, or 18 or a functional fragment of the nucleotide sequence set forth in SEQ ID NO:1, or 18, to a heterologous nucleic acid fragment.

[0094] Another embodiment is a method for transforming a cell (or microorganism) comprising transforming a cell (or microorganism) with any of the isolated polynucleotides or recombinant DNA constructs of the present invention. The cell (or microorganism) transformed by this method is also included. In particular embodiments, the cell is eukaryotic cell, e.g., a yeast, insect or plant cell, or prokaryotic, e.g., a bacterial cell. The microorganism may be Agrobacterium, e.g. Agrobacterium tumefaciens or Agrobacterium rhizogenes.

[0095] Another embodiment of this invention is a method of expressing a heterologous polynucleotide in a plant, comprising the steps of introducing into a regenerable plant cell the recombinant DNA construct described above and regenerating a transgenic plant from the transformed regenerable plant cell, wherein the transgenic plant comprises the recombinant DNA construct and exhibits expression of the heterologous polynucleotide.

[0096] Another embodiment of this invention is a method of expressing a heterologous polynucleotide in a plant, comprising the steps of introducing into a regenerable plant cell the recombinant DNA construct described above; regenerating a transgenic plant from the regenerable plant cell described above; and obtaining a progeny plant from the transgenic plant, wherein the transgenic plant and the progeny plant comprises the recombinant DNA construct and exhibits expression of the heterologous polynucleotide.

[0097] In another embodiment, any of the methods of expressing a heterologous polynucleotide, wherein the plant cell is a monocotyledonous or dicotyledonous plant cell, for example, a maize or soybean plant cell. The plant cell may also be from sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, millet, sugar cane or switchgrass.

[0098] In another embodiment, this invention concerns a vector, virus, cell, microorganism, plant, or seed comprising a recombinant DNA construct comprising the terminator sequences described in the present invention.

[0099] The invention encompasses regenerated, mature and fertile transgenic plants comprising the recombinant DNA constructs described above, transgenic seeds produced therefrom, T1 and subsequent generations. The transgenic plant cells, tissues, plants, and seeds may comprise at least one recombinant DNA construct of interest.

[0100] In one embodiment, the plant (or seed derived from the plant) comprising the terminator sequences described in the present invention is a monocotyledonous or dicotyledonous plant, for example, a maize or soybean plant. The plant may also be sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, millet, sugar cane or switchgrass. The plant may be an inbred plant or a hybrid plant.

EXAMPLES

[0101] The present invention is further illustrated in the following Examples, in which parts and percentages are by weight and degrees are Celsius, unless otherwise stated. It should be understood that these examples, while indicating embodiments of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Furthermore, various modifications of the invention in addition to those shown and described herein will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims.

Example 1

Amplification and Cloning of a Sorghum bicolor Gamma-Kafirin Terminator Sequence

[0102] Primers (SEQ ID NOS:2 and 3) were designed for amplifying the terminator of gamma-Kafirin gene from Sorghum bicolor (SB-GKAF) based on the Sorghum bicolor genomic sequence database. The primer sequences are given below, the underlined region is not homologous with genomic template:

TABLE-US-00001 TMS2039 (forward primer; SEQ ID NO: 2): CAGATCTGATATCGATGGGCCCACTAACTATCTATACTGTAATAATGTT GTATAG TMS2040 (reverse primer; SEQ ID NO: 3): CGGACCGGGTGACCAAGCTTAAGCGAACATATGTCCCTC

[0103] A 504 bp product comprising the 465 bp SB-GKAF terminator sequence (SEQ ID NO:1) was amplified by PCR using these primers. The product was cloned into pGEMTeasy (Promega) (PHP31801; FIG. 1; SEQ ID NO:4) and the sequence was confirmed. The cloned SB-GKAF terminator included 165 bp of the predicted 3' UTR of SB-GKAF along with about 300 bp of downstream sequence. The amplified sequence of SB-GKAF terminator (SEQ ID NO:1) was then cloned into an Agrobacterium transformation vector (PHP34074; FIG.2; SEQ ID NO:5), which had the following expression cassettes in divergent orientation:

[0104] SB-GKAF TERMINATOR: GUSINT: BSV PRO and

[0105] UBI-PRO:UBI INTRON:MOPAT:PINII TERM.

[0106] BSV PRO is Banana Streak Virus promoter, which is a strong constitutive promoter. A construct with a potato PINII terminator (Keil et al. (1986) Nucleic Acids Res. 14:5641-5650) in place of the SB-GKAF terminator was used as a control (PHP34005; SEQ ID NO:6).

Example 2

Transient Transformation to Test Efficacy of a SB-GKAF Terminator

[0107] The isolated SB-GKAF terminator sequence (SEQ ID NO:1) was tested for its ability to act efficiently as a terminator in a recombinant construct. Its efficacy as a terminator was tested by its ability to stop transcription and by its ability to increase expression of a protein. Since improper termination can lead to improper processing of the 3' end of mRNA, and hence affect RNA stability, terminators have been found to affect protein expression levels. It has been shown that different terminators can cause up to 100-fold variation in the efficiency of transgene expression (Bieri et al, (2002) Molecular Breeding 10: 107-117; An et al (1989) Plant Cell 1: 115-122; Ingelbrecht et al (1989), Plant Cell, 1:671-680; Ali and Taylor (2001) Plant Mol. Bio., 46:251-261). Hence we tested the SB-GKAF sequence (SEQ ID NO:1) for its ability to increase expression of a protein compared to the well-known PINII terminator. The Agrobacterium transformation vectors PHP34074 (SEQ ID NO:5) and PHP34005 (SEQ ID NO:6) described in Example 1 were used for transient transformation of BMS (Black Mexican Sweet) cells. The cells were harvested 5 days after transformation and sent for a quantification of the GUS activity (MUG assay). The SB-GKAF construct (PHP34074; SEQ ID NO:5) had .about.35% more expression than that of the PINII construct (PHP34005, SEQ ID NO:6) when the GUS expression was normalized to the MOPAT expression (FIG. 3; Table 1). This information was indicative of the ability of the isolated SB-GKAF sequence (SEQ ID NO:1) to act efficiently as a terminator, by allowing protein expression equal to or above that of the PINII terminator.

TABLE-US-00002 TABLE 1 Sequence Average Standard Construct Tested MUG/PAT* Deviation BSV PRO: GUSINT: PIN II 1.57 0.17 PIN II TERM TERM BSV PRO: GUSINT: SB-GKAF 2.13 0.41 SB-GKAF TERM TERM *Measured as: nmoles MU/mg total protein/hour/ppm PAT

Example 3

Stable Transformation Assays to Test SB-GKAF Terminator Activity

[0108] The Agrobacterium transformation vectors PHP34074 (SEQ ID NO:5) and PHP34005 (SEQ ID NO:6) described in Example 1, that were used for transient transformation assays as described in Example 2, were also used in Gaspe-Flint derived maize lines for stable transformation to generate transgenic maize plants.

[0109] Quantitative Reverse Transcriptase-PCR (qRT-PCR) and GUS assays were done from stably transformed plant tissues to test the ability of isolated SB-GKAF terminator sequence (SEQ ID NO:1) to stop transcription (that is prevent transcription read-through transcription) and to compare GUS expression as compared to that with PINII terminator.

GUS Expression Analysis:

[0110] The expression of the GUS gene in the transgenic plants was assessed at the protein as well as transcript levels. To assess the expression at the protein level, MUG assay was performed on seedling leaf material. To assess the expression at the transcript level, qRT-PCR was done using primers shown in Table 2.

TABLE-US-00003 TABLE 2 Primer/ qPCR Probe Type Sequence Fluor Assay GUS-1482F Forward SEQ ID NO: 7 -- Taqman GUS-1553R Reverse SEQ ID NO: 8 -- Taqman GUS-1509P Probe SEQ ID NO: 9 FAM Taqman

[0111] Plants were grown in the greenhouse and leaves were sampled at the R1 stage of development for expression analysis. Multiple plants were tested for each construct. Each plant was analyzed for expression of the GUS gene. GUS gene with the SB-GKAF terminator had GUS expression in the same range as that of PINII terminator at both the protein (FIG. 4A) and transcript (FIG. 4B) level.

Quantitative Reverse Transcriptase PCR (qRT-PCR) to Determine Read-Through Transcription Through the SB-GKAF Terminator:

[0112] The qRT-PCR assays were performed with leaf tissue from the stable transformants generated using PHP34074 and PHP34005. Each plant was tested for the presence of read-through transcript that had passed through the PIN II terminator and the SB-GKAF terminator (SEQ ID NO:1). To assess presence of products that would indicate that transcription was continuing past the terminator, amplification was targeted downstream of the terminator being tested. Two primer sets were designed downstream of the tested terminators.

[0113] Primer set Term1 .about.100 nt from the terminator

[0114] Primer set Term2.1 .about.500 nt from the terminator

[0115] Multiple plants were tested for each construct. The primers are shown in Table 3.

TABLE-US-00004 TABLE 3 Primer/ qPCR Probe Name Type Sequence Fluor Assay Term2.1.sup.1 Term2.1F fwd SEQ ID NO: 10 -- SYBR Term2.1.sup.1 Term2.1R rev SEQ ID NO: 11 -- SYBR Term1.sup.1 Term_1F fwd SEQ ID NO: 12 -- Taqman Term1.sup.1 Term_1R rev SEQ ID NO: 13 -- Taqman Term1.sup.1 Term_1P probe SEQ ID NO: 14 FAM Taqman Actin.sup.2 Actin_ fwd SEQ ID NO: 15 -- Taqman MGB_F Actin.sup.2 Actin_ rev SEQ ID NO: 16 -- Taqman MGB_R Actin.sup.2 Actin_ probe SEQ ID NO: 17 VIC Taqman VIC_P .sup.1Post-Terminator Primer Set .sup.2Reference Gene

[0116] The test plants were classified into 3 categories depending on the qRT-PCR results:

[0117] 1. Plants showing complete termination: where all GUS transcripts are completely terminated before they reached the specific primer set location;

[0118] 2. Plants showing a high degree of termination: where a large portion of the GUS transcripts are terminated before they reached the specific primer set location, also defined as:

[0119] Primer set Term1--.DELTA.CT>13

[0120] Primer set Term2.1--.DELTA.CT>9; and

[0121] 3. Plants showing poor termination.

[0122] As can be see from FIG. 5, the SB-GKAF terminator proved to have fewer "poorly terminating" plants than the PINII terminator (FIG. 5). Thus the qRT-PCR score for presence of transcripts that had proceeded through the terminator was lower for the SB-GKAF terminator than that for the PINII terminator.

Sequence CWU 1

1

191459DNASorghum bicolor 1aactatctat actgtaataa tgttgtatag ccgccggata gctagctagt ttagtcattc 60agcggcgatg ggtaataata aagtgtcatc catccatcac catgggtggc aacgtgagca 120atgacctgat tgaacaaatt gaaatgaaaa gaagaaatat gttatatgtc aacgagattt 180cctcataatg ccactgacaa cgtgtgtcca agaaatgtat cagtgatacg tatattcaca 240atttttttat gacttatact cacaatttgt ttttttacta cttatactca caatttgttg 300tgggtaccat aacaatttcg atcgaatata tatcagaaag ttgacgaaag taagctcact 360caaaaagtta aatgggctgc ggaagctgcg tcaggcccaa gttttggcta ttctatccgg 420tatccacgat tttgatggct gagggacata tgttcgctt 459255DNAArtificial Sequenceforward primer 2cagatctgat atcgatgggc ccactaacta tctatactgt aataatgttg tatag 55339DNAArtificial SequenceReverse primer 3cggaccgggt gaccaagctt aagcgaacat atgtccctc 3943521DNAArtificial SequenceVector 4gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 60tcggaccggg tgaccaagct taagcgaaca tatgtccctc agccatcaaa atcgtggata 120ccggatagaa tagccaaaac ttgggcctga cgcagcttcc gcagcccatt taactttttg 180agtgagctta ctttcgtcaa ctttctgata tatattcgat cgaaattgtt atggtaccca 240caacaaattg tgagtataag tagtaaaaaa acaaattgtg agtataagtc ataaaaaaat 300tgtgaatata cgtatcactg atacatttct tggacacacg tcgtcagtgg cattatgagg 360aaatctcgtt gacatataac atatttcttc ttttcatttc aatttgttca atcaggtcat 420tgctcacgtt gccacccatg gtgatggatg gatgacactt tattattacc catcgccgct 480gaatgactaa actagctagc tatccggcgg ctatacaaca ttattacagt atagatagtt 540agtgggccca tcgatatcag atctgaatca ctagtgaatt cgcggccgcc tgcaggtcga 600ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct 660aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 720aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 780gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 840gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 900ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 960atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 1020gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 1080gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 1140gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 1200gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 1260aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 1320ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 1380taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 1440tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 1500gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 1560taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 1620tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 1680tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 1740ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 1800taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 1860tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 1920cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 1980gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 2040cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 2100ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 2160aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 2220atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 2280tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 2340gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 2400aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 2460acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 2520ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 2580tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 2640aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 2700catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 2760atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 2820aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 2880tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 2940ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 3000cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 3060ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 3120accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 3180gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 3240gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 3300caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg 3360cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 3420gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 3480tgtaaaacga cggccagtga attgtaatac gactcactat a 3521550910DNAArtificial SequenceSB-GKAF terminator construct 5acgtgaccct agtcacttag gttaccagag ctggtcacct ttgtccacca agatggaact 60gcggccgctc attaattaag tcaggcgcgc ctctagttga agacacgttc atgtcttcat 120cgtaagaaga cactcagtag tcttcggcca gaatggccat ctggattcag caggcctaga 180aggccattta aatcctgagg atctggtctt cctaaggacc cgggatatcg ctatcaactt 240tgtatagaaa agttgggccg aattcgagct cggtacggcc agaatggccc ggaccgggtt 300accgaattcg agctcggtac cactagtaag cttaagcgaa catatgtccc tcagccatca 360aaatcgtgga taccggatag aatagccaaa acttgggcct gacgcagctt ccgcagccca 420tttaactttt tgagtgagct tactttcgtc aactttctga tatatattcg atcgaaattg 480ttatggtacc cacaacaaat tgtgagtata agtagtaaaa aaacaaattg tgagtataag 540tcataaaaaa attgtgaata tacgtatcac tgatacattt cttggacaca cgtcgtcagt 600ggcattatga ggaaatctcg ttgacatata acatatttct tcttttcatt tcaatttgtt 660caatcaggtc attgctcacg ttgccaccca tggtgatgga tggatgacac tttattatta 720cccatcgccg ctgaatgact aaactagcta gctatccggc ggctatacaa cattattaca 780gtatagatag ttagtgggcc catcgatatc agatcttcat tgtttgcctc cctgctgcgg 840tttttcaccg aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg 900tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga 960ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa 1020gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta 1080cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg 1140cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc 1200gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct 1260aatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg 1320gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg 1380ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc 1440tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc 1500gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atccctaaag agaggttaaa 1560gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat 1620ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa 1680tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgcaagt ccgcatcttc 1740atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt 1800cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt 1860ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac 1920cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg 1980cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca 2040gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta 2100gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt 2160tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc 2220gttgttcaca caaacggtga tacctgcaca tcaacaaatt ttggtcatat attagaaaag 2280ttataaatta aaatatacac acttataaac tacagaaaag caattgctat atactacatt 2340cttttatttt gaaaaaaata tttgaaatat tatattacta ctaattaatg ataattatta 2400tatatatatc aaaggtagaa gcagaaactt acgtacactt ttcccggcaa taacatacgg 2460cgtgacatcg gcttcaaatg gcgtatagcc gccctgatgc tccatcactt cctgattatt 2520gacccacact ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac gctggcctgc 2580ccaacctttc ggtataaaga cttcgcgctg ataccagacg ttgcccgcat aattacgaat 2640atctgcatcg gcgaactgat cgttaaaact gcctggcaca gcaattgccc ggctttcttg 2700taacgcgctt tcccaccaac gctgatcaat tccacagttt tcgcgatcca gactgaatgc 2760ccacaggccg tcgagttttt tgatttcacg ggttggggtt tctacaggac ggaccatggt 2820gtcgtgtgga tccaaattgt atgcaaggtg aatgactttc ttttcgtaaa ctagatagga 2880gtactcctcc aggatgctta acccgtattg acgtacagag gtctatgatc cttttgttta 2940taaaggagct tgtagttcag tcagtcttat acttcacgat gcccatgttt ctatatagga 3000tattatcttg gctttgtaag tacttcacgc aggttatgtt ctgtttctag gatattatcc 3060tcatacatgc gaagaaccaa tttttccccc attctcttcg ggtacttttt cttgggtagg 3120catgctctct tggaccaact agcataaaac ataatcattt ttccctacag ccttgaccag 3180ctataatcga aatcatgctc atttttctaa gaaagactga atacagctcc aatttaaaca 3240atttaaatca taaacttgta actcaattag agaaaagcag agcccttcgg ctcctatcta 3300aaggaattac cccatgaaag ccataaaaac gaaccttgct ctgataccag acgggtctac 3360gctcgcggaa ctaggatctt gcgctctact cgcacaaagt gaactcgcac aaagtgtgtt 3420tcaagcacag aagtttttat ttctcaaatc aggagtaaac tcgcgttgtg gtgcgtgttt 3480gcaacctgaa tacaaggctc cttatataga gagttgtgga gctttctggc atcgttaggt 3540ggcatccacc aataatgcag ataagcatca tcacatgtct ctggcctaac aactttgcgt 3600aagaatcctg caaagttact aaaggtcatc gtgcgtgact agacaacgca caccgacaaa 3660cttaaaataa agagacatta tactttgtct cctctttaca taaagtgagt ggtatccagc 3720tcactccgca tcttatcagt cttcacaccg gttggtatca acacgtggta ggggtccgcc 3780acttccgctt cagtcatcat tactgatatc cagcagatct agagcatctt caataagata 3840ttcttgttct gcacgcagat tttcttgctc cctcagtaat tcctcccaca gtgagtcttc 3900tgatatttct tcaagtttct tctcccatct gatcttttcc tgcacaaacg agtcaatttg 3960gtctttccag acccaagtaa aacaagtgtt agtttcacag gagtaaaact ccctgtcagg 4020atttctggat gttctggaga tcttcagttt tgctggttta ttgcatccac atttgaaaac 4080cggctcttca cttagtgtta gcacattgat ttgatgcaac ctgtagcctt tgctcaacca 4140gtcttcatat ctttttacaa catcattaac tctctgtttt gcatcggtgt ttcccttgtg 4200aaatacctcc tccactgcat tgatcaacac accttcagat tgatgctttt ccggatggag 4260aataatcttt accagtcttg acagagtgtc tgctaaaacg ttgtcctttc cgtcaatgtg 4320ttcaaactta atctcaagac ctgtcccggt aatgtaatct gtgaaggcaa gccatctgac 4380tcttgatggt ttatgatcac tgcttttctt gtaaaagctc actattgctt gactgtcagt 4440tctgattatg agctctttgt aagcttggtc acccggtccg ggcctagaag gccagcttcg 4500gccgccccgg gcaactttat tatacaaagt tgatagatat cggaccgatt aaactttaat 4560tcggtccgaa gcttgcatgc ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga 4620taatgagcat tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg 4680tttgaagtgc agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat 4740aatctatagt actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac 4800atggtctaaa ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt 4860gtgcatgtgt tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt 4920tattagtaca tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta 4980catctatttt attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt 5040tttatttaat aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat 5100accctttaag aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc 5160agcctgttaa acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc 5220gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga 5280gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg cgtggcggag 5340cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca ccggcagcta 5400cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg taataaatag 5460acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac acacacacaa 5520ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc 5580cccccccccc ctctctacct tctctagatc ggcgttccgg tccatgcatg gttagggccc 5640ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc 5700tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag 5760tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatttca 5820tgattttttt tgtttcgttg catagggttt ggtttgccct tttcctttat ttcaatatat 5880gccgtgcact tgtttgtcgg gtcatctttt catgcttttt tttgtcttgg ttgtgatgat 5940gtggtctggt tgggcggtcg ttctagatcg gagtagaatt ctgtttcaaa ctacctggtg 6000gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag 6060atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg 6120catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg 6180ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt 6240ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat 6300atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc 6360atatgcagca tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt 6420atgttttata attattttga tcttgatata cttggatgat ggcatatgca gcagctatat 6480gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt 6540cgatgctcac cctgttgttt ggtgttactt ctgcaggtcg actttaactt agcctaggat 6600ccacacgaca ccatgtcccc cgagcgccgc cccgtcgaga tccgcccggc caccgccgcc 6660gacatggccg ccgtgtgcga catcgtgaac cactacatcg agacctccac cgtgaacttc 6720cgcaccgagc cgcagacccc gcaggagtgg atcgacgacc tggagcgcct ccaggaccgc 6780tacccgtggc tcgtggccga ggtggagggc gtggtggccg gcatcgccta cgccggcccg 6840tggaaggccc gcaacgccta cgactggacc gtggagtcca ccgtgtacgt gtcccaccgc 6900caccagcgcc tcggcctcgg ctccaccctc tacacccacc tcctcaagag catggaggcc 6960cagggcttca agtccgtggt ggccgtgatc ggcctcccga acgacccgtc cgtgcgcctc 7020cacgaggccc tcggctacac cgcccgcggc accctccgcg ccgccggcta caagcacggc 7080ggctggcacg acgtcggctt ctggcagcgc gacttcgagc tgccggcccc gccgcgcccg 7140gtgcgcccgg tgacgcagat ctgagtcgaa acctagactt gtccatcttc tggattggcc 7200aacttaatta atgtatgaaa taaaaggatg cacacatagt gacatgctaa tcactataat 7260gtgggcatca aagttgtgtg ttatgtgtaa ttactagtta tctgaataaa agagaaagag 7320atcatccata tttcttatcc taaatgaatg tcacgtgtct ttataattct ttgatgaacc 7380agatgcattt cattaaccaa atccatatac atataaatat taatcatata taattaatat 7440caattgggtt agcaaaacaa atctagtcta ggtgtgtttt gcgaatgcgg ccgataagtg 7500actagggtca cgtgacccta gtcacttagg taccgagctc gaattcattc cgattaatcg 7560tggcctcttg ctcttcagga tgaagagcta tgtttaaacg tgcaagcgct actagacaat 7620tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac 7680accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa 7740atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt 7800aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc 7860gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc 7920gttgctgcct gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta 7980ttcatttctc gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg 8040aaatcgctgg ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt 8100tgacgatcgt cgaccgtacc ccgatgaatt aattcggacg tacgttctga acacagctgg 8160atacttactt gggcgattgt catacatgac atcaacaatg tacccgtttg tgtaaccgtc 8220tcttggaggt tcgtatgaca ctagtggttc ccctcagctt gcgactagat gttgaggcct 8280aacattttat tagagagcag gctagttgct tagatacatg atcttcaggc cgttatctgt 8340cagggcaagc gaaaattggc catttatgac gaccaatgcc ccgcagaagc tcccatcttt 8400gccgccatag acgccgcgcc ccccttttgg ggtgtagaac atccttttgc cagatgtgga 8460aaagaagttc gttgtcccat tgttggcaat gacgtagtag ccggcgaaag tgcgagaccc 8520atttgcgcta tatataagcc tacgatttcc gttgcgacta ttgtcgtaat tggatgaact 8580attatcgtag ttgctctcag agttgtcgta atttgatgga ctattgtcgt aattgcttat 8640ggagttgtcg tagttgcttg gagaaatgtc gtagttggat ggggagtagt catagggaag 8700acgagcttca tccactaaaa caattggcag gtcagcaagt gcctgccccg atgccatcgc 8760aagtacgagg cttagaacca ccttcaacag atcgcgcata gtcttcccca gctctctaac 8820gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta gacattattt 8880gccgactacc ttggtgatct cgcctttcac gtagtgaaca aattcttcca actgatctgc 8940gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc ctagcttcaa gtatgacggg 9000ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat 9060tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc 9120gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag 9180atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct 9240atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa 9300gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg 9360ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat 9420ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt 9480tgtttcatca agccttacag tcaccgtaac cagcaaatca atatcactgt gtggcttcag 9540gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg 9600ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct 9660catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg gtgtcggctt gaatgaattg 9720ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac atcgctgttt cgttcgagac 9780ttgaggtcta gttttatacg tgaacaggtc aatgccgccg agagtaaagc cacattttgc 9840gtacaaattg caggcaggta cattgttcgt ttgtgtctct aatcgtatgc caaggagctg 9900tctgcttagt gcccactttt tcgcaaattc gatgagactg tgcgcgactc ctttgcctcg 9960gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga tcgttccatg ttgagttgag 10020ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca tagcaagcag agtcttcatc 10080agagtcatca tccgagatgt aatccttccg gtaggggctc acacttctgg tagatagttc 10140aaagccttgg tcggataggt gcacatcgaa cacttcacga acaatgaaat ggttctcagc 10200atccaatgtt tccgccacct gctcagggat caccgaaatc ttcatatgac gcctaacgcc 10260tggcacagcg gatcgcaaac ctggcgcggc ttttggcaca aaaggcgtga caggtttgcg 10320aatccgttgc tgccacttgt taaccctttt gccagatttg gtaactataa tttatgttag 10380aggcgaagtc ttgggtaaaa actggcctaa aattgctggg gatttcagga aagtaaacat 10440caccttccgg ctcgatgtct attgtagata tatgtagtgt atctacttga tcgggggatc 10500tgctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 10560acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 10620gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 10680tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt

10740gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 10800cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 10860aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 10920aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 10980tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 11040caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 11100cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 11160ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 11220gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 11280agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 11340gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 11400acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 11460gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 11520gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 11580cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 11640caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 11700gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 11760cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 11820cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 11880caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 11940gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 12000gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggg gggggggggg 12060gggggttcca ttgttcattc cacggacaaa aacagagaaa ggaaacgaca gaggccaaaa 12120agctcgcttt cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat 12180taagttatga cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa 12240aacccgcgag gtcgccgccc cgtaacctgt cggatcaccg gaaaggaccc gtaaagtgat 12300aatgattatc atctacatat cacaacgtgc gtggaggcca tcaaaccacg tcaaataatc 12360aattatgacg caggtatcgt attaattgat ctgcatcaac ttaacgtaaa aacaacttca 12420gacaatacaa atcagcgaca ctgaatacgg ggcaacctca tgtccccccc cccccccccc 12480ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 12540aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 12600gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 12660cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 12720actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 12780caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 12840gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 12900ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 12960caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 13020tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 13080gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 13140cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 13200ataggcgtat cacgaggccc tttcgtcttc aagaattcgg agcttttgcc attctcaccg 13260gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 13320ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 13380atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 13440tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 13500ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg 13560gacggcggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc 13620ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca 13680cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga 13740ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag cgccagaagg 13800ccgccagaga ggccgagcgc ggccgtgagg cttggacgct agggcagggc atgaaaaagc 13860ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg gggaggggat gttgtctaca 13920tggctctgct gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat cgtcaccctt 13980tctcggtcct tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac 14040cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc 14100ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct 14160gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc 14220attgacggcg tccccggccg aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc 14280cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta 14340ggcgagcagc gcctgcctga agctgcgggc attcccgatc agaaatgagc gccagtcgtc 14400gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc 14460gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg 14520ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc 14580ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat cactgataaa 14640cataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg 14700aggctggtcc ggaggccaga cgtgaaaccc aacatacccc tgatcgtaat tctgagcact 14760gtcgcgctcg acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc 14820gatctggttc actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg 14880ttggtgcaat ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg 14940ccaatcttgc tcgtctcgct ggccggcgcc actgtcgact acgccatcat ggcgacagcg 15000cctttccttt gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg 15060gcggtagccg gcgcttatat tgccgatatc actgatggcg atgagcgcgc gcggcacttc 15120ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct cggtgggctg 15180atgggcggtt tctcccccca cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat 15240ttcctgacgg gctgtttcct tttgccggag tcgcacaaag gcgaacgccg gccgttacgc 15300cgggaggctc tcaacccgct cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc 15360gccctgatgg cggtcttctt catcatgcaa cttgtcggac aggtgccggc cgcgctttgg 15420gtcattttcg gcgaggatcg ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc 15480gcatttggca ttctgcattc actcgcccag gcaatgatca ccggccctgt agccgcccgg 15540ctcggcgaaa ggcgggcact catgctcgga atgattgccg acggcacagg ctacatcctg 15600cttgccttcg cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt 15660ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag 15720gggcagctgc aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc 15780ctcttcacgg cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca 15840ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca 15900gggcaacgag ccgatcgctg atcgtggaaa cgataggcct atgccatgcg ggtcaaggcg 15960acttccggca agctatacgc gccctaggag tgcggttgga acgttggccc agccagatac 16020tcccgatcac gagcaggacg ccgatgattt gaagcgcact cagcgtctga tccaagaaca 16080accatcctag caacacggcg gtccccgggc tgagaaagcc cagtaaggaa acaactgtag 16140gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc 16200cgagccacgc caggccgaga acattggttc ctgtaggcat cgggattggc ggatcaaaca 16260ctaaagctac tggaacgagc agaagtcctc cggccgccag ttgccaggcg gtaaaggtga 16320gcagaggcac gggaggttgc cacttgcggg tcagcacggt tccgaacgcc atggaaaccg 16380cccccgccag gcccgctgcg acgccgacag gatctagcgc tgcgtttggt gtcaacacca 16440acagcgccac gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc 16500tacctagcag agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg 16560ccgcgacccc gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa 16620tattaagtgc gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga 16680cgatcatcac gagcaataaa cccgccggca acgcccgcag cagcataccg gcgacccctc 16740ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg cgccttgtga gcgtccttgg 16800ggccgtcctc ctgtttgaag accgacagcc caatgatctc gccgtcgatg taggcgccga 16860atgccacggc atctcgcaac cgttcagcga acgcctccat gggctttttc tcctcgtgct 16920cgtaaacgga cccgaacatc tctggagctt tcttcagggc cgacaatcgg atctcgcgga 16980aatcctgcac gtcggccgct ccaagccgtc gaatctgagc cttaatcaca attgtcaatt 17040ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc gatactgagc 17100gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag cgctggctgc 17160tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc 17220attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac 17280ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc 17340cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt 17400cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag 17460cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag 17520gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa 17580gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc 17640attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc 17700gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc 17760ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt 17820gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc 17880cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag 17940ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct 18000gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg 18060cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg 18120ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc 18180gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc 18240acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat 18300cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat 18360tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc ctggtgcctt 18420ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc 18480cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa 18540atacttgccg tgggcctcgg cctgagagcc aaaacacttg atgcggaaga agtcggtgcg 18600ctcctgcttg tcgccggcat cgttgcgcca ctcttcatta accgctatat cgaaaattgc 18660ttgcggcttg ttagaattgc catgacgtac ctcggtgtca cgggtaagat taccgataaa 18720ctggaactga ttatggctca tatcgaaagt ctccttgaga aaggagactc tagtttagct 18780aaacattggt tccgctgtca agaactttag cggctaaaat tttgcgggcc gcgaccaaag 18840gtgcgagggg cggcttccgc tgtgtacaac cagatatttt tcaccaacat ccttcgtctg 18900ctcgatgagc ggggcatgac gaaacatgag ctgtcggaga gggcaggggt ttcaatttcg 18960tttttatcag acttaaccaa cggtaaggcc aacccctcgt tgaaggtgat ggaggccatt 19020gccgacgccc tggaaactcc cctacctctt ctcctggagt ccaccgacct tgaccgcgag 19080gcactcgcgg agattgcggg tcatcctttc aagagcagcg tgccgcccgg atacgaacgc 19140atcagtgtgg ttttgccgtc acataaggcg tttatcgtaa agaaatgggg cgacgacacc 19200cgaaaaaagc tgcgtggaag gctctgacgc caagggttag ggcttgcact tccttcttta 19260gccgctaaaa cggccccttc tctgcgggcc gtcggctcgc gcatcatatc gacatcctca 19320acggaagccg tgccgcgaat ggcatcgggc gggtgcgctt tgacagttgt tttctatcag 19380aacccctacg tcgtgcggtt cgattagctg tttgtcttgc aggctaaaca ctttcggtat 19440atcgtttgcc tgtgcgataa tgttgctaat gatttgttgc gtaggggtta ctgaaaagtg 19500agcgggaaag aagagtttca gaccatcaag gagcgggcca agcgcaagct ggaacgcgac 19560atgggtgcgg acctgttggc cgcgctcaac gacccgaaaa ccgttgaagt catgctcaac 19620gcggacggca aggtgtggca cgaacgcctt ggcgagccga tgcggtacat ctgcgacatg 19680cggcccagcc agtcgcaggc gattatagaa acggtggccg gattccacgg caaagaggtc 19740acgcggcatt cgcccatcct ggaaggcgag ttccccttgg atggcagccg ctttgccggc 19800caattgccgc cggtcgtggc cgcgccaacc tttgcgatcc gcaagcgcgc ggtcgccatc 19860ttcacgctgg aacagtacgt cgaggcgggc atcatgaccc gcgagcaata cgaggtcatt 19920aaaagcgccg tcgcggcgca tcgaaacatc ctcgtcattg gcggtactgg ctcgggcaag 19980accacgctcg tcaacgcgat catcaatgaa atggtcgcct tcaacccgtc tgagcgcgtc 20040gtcatcatcg aggacaccgg cgaaatccag tgcgccgcag agaacgccgt ccaataccac 20100accagcatcg acgtctcgat gacgctgctg ctcaagacaa cgctgcgtat gcgccccgac 20160cgcatcctgg tcggtgaggt acgtggcccc gaagcccttg atctgttgat ggcctggaac 20220accgggcatg aaggaggtgc cgccaccctg cacgcaaaca accccaaagc gggcctgagc 20280cggctcgcca tgcttatcag catgcacccg gattcaccga aacccattga gccgctgatt 20340ggcgaggcgg ttcatgtggt cgtccatatc gccaggaccc ctagcggccg tcgagtgcaa 20400gaaattctcg aagttcttgg ttacgagaac ggccagtaca tcaccaaaac cctgtaagga 20460gtatttccaa tgacaacggc tgttccgttc cgtctgacca tgaatcgcgg cattttgttc 20520taccttgccg tgttcttcgt tctcgctctc gcgttatccg cgcatccggc gatggcctcg 20580gaaggcaccg gcggcagctt gccatatgag agctggctga cgaacctgcg caactccgta 20640accggcccgg tggccttcgc gctgtccatc atcggcatcg tcgtcgccgg cggcgtgctg 20700atcttcggcg gcgaactcaa cgccttcttc cgaaccctga tcttcctggt tctggtgatg 20760gcgctgctgg tcggcgcgca gaacgtgatg agcaccttct tcggtcgtgg tgccgaaatc 20820gcggccctcg gcaacggggc gctgcaccag gtgcaagtcg cggcggcgga tgccgtgcgt 20880gcggtagcgg ctggacggct cgcctaatca tggctctgcg cacgatcccc atccgtcgcg 20940caggcaaccg agaaaacctg ttcatgggtg gtgatcgtga actggtgatg ttctcgggcc 21000tgatggcgtt tgcgctgatt ttcagcgccc aagagctgcg ggccaccgtg gtcggtctga 21060tcctgtggtt cggggcgctc tatgcgttcc gaatcatggc gaaggccgat ccgaagatgc 21120ggttcgtgta cctgcgtcac cgccggtaca agccgtatta cccggcccgc tcgaccccgt 21180tccgcgagaa caccaatagc caagggaagc aataccgatg atccaagcaa ttgcgattgc 21240aatcgcgggc ctcggcgcgc ttctgttgtt catcctcttt gcccgcatcc gcgcggtcga 21300tgccgaactg aaactgaaaa agcatcgttc caaggacgcc ggcctggccg atctgctcaa 21360ctacgccgct gtcgtcgatg acggcgtaat cgtgggcaag aacggcagct ttatggctgc 21420ctggctgtac aagggcgatg acaacgcaag cagcaccgac cagcagcgcg aagtagtgtc 21480cgcccgcatc aaccaggccc tcgcgggcct gggaagtggg tggatgatcc atgtggacgc 21540cgtgcggcgt cctgctccga actacgcgga gcggggcctg tcggcgttcc ctgaccgtct 21600gacggcagcg attgaagaag agcgctcggt cttgccttgc tcgtcggtga tgtacttcac 21660cagctccgcg aagtcgctct tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg 21720caccccccgg ccgttttagc ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc 21780ccatcatgac cttgccaagc tcgtcctgct tctcttcgat cttcgccagc agggcgagga 21840tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc 21900cgcccaggcg gcccaggtcg ccattgatgc gggccagctc gcggacgtgc tcatagtcca 21960cgacgcccgt gattttgtag ccctggccga cggccagcag gtaggccgac aggctcatgc 22020cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga 22080taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg ccctcatctg 22140ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt 22200gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc 22260tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc 22320ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc 22380agggttatgc agcggaaaag cgctgcttcc ctgctgtttt gtggaatatc taccgactgg 22440aaacaggcaa atgcaggaaa ttactgaact gaggggacag gcgagagacg atgccaaaga 22500gctacaccga cgagctggcc gagtgggttg aatcccgcgc ggccaagaag cgccggcgtg 22560atgaggctgc ggttgcgttc ctggcggtga gggcggatgt cgaggcggcg ttagcgtccg 22620gctatgcgct cgtcaccatt tgggagcaca tgcgggaaac ggggaaggtc aagttctcct 22680acgagacgtt ccgctcgcac gccaggcggc acatcaaggc caagcccgcc gatgtgcccg 22740caccgcaggc caaggctgcg gaacccgcgc cggcacccaa gacgccggag ccacggcggc 22800cgaagcaggg gggcaaggct gaaaagccgg cccccgctgc ggccccgacc ggcttcacct 22860tcaacccaac accggacaaa aaggatctac tgtaatggcg aaaattcaca tggttttgca 22920gggcaagggc ggggtcggca agtcggccat cgccgcgatc attgcgcagt acaagatgga 22980caaggggcag acacccttgt gcatcgacac cgacccggtg aacgcgacgt tcgagggcta 23040caaggccctg aacgtccgcc ggctgaacat catggccggc gacgaaatta actcgcgcaa 23100cttcgacacc ctggtcgagc tgattgcgcc gaccaaggat gacgtggtga tcgacaacgg 23160tgccagctcg ttcgtgcctc tgtcgcatta cctcatcagc aaccaggtgc cggctctgct 23220gcaagaaatg gggcatgagc tggtcatcca taccgtcgtc accggcggcc aggctctcct 23280ggacacggtg agcggcttcg cccagctcgc cagccagttc ccggccgaag cgcttttcgt 23340ggtctggctg aacccgtatt gggggcctat cgagcatgag ggcaagagct ttgagcagat 23400gaaggcgtac acggccaaca aggcccgcgt gtcgtccatc atccagattc cggccctcaa 23460ggaagaaacc tacggccgcg atttcagcga catgctgcaa gagcggctga cgttcgacca 23520ggcgctggcc gatgaatcgc tcacgatcat gacgcggcaa cgcctcaaga tcgtgcggcg 23580cggcctgttt gaacagctcg acgcggcggc cgtgctatga gcgaccagat tgaagagctg 23640atccgggaga ttgcggccaa gcacggcatc gccgtcggcc gcgacgaccc ggtgctgatc 23700ctgcatacca tcaacgcccg gctcatggcc gacagtgcgg ccaagcaaga ggaaatcctt 23760gccgcgttca aggaagagct ggaagggatc gcccatcgtt ggggcgagga cgccaaggcc 23820aaagcggagc ggatgctgaa cgcggccctg gcggccagca aggacgcaat ggcgaaggta 23880atgaaggaca gcgccgcgca ggcggccgaa gcgatccgca gggaaatcga cgacggcctt 23940ggccgccagc tcgcggccaa ggtcgcggac gcgcggcgcg tggcgatgat gaacatgatc 24000gccggcggca tggtgttgtt cgcggccgcc ctggtggtgt gggcctcgtt atgaatcgca 24060gaggcgcaga tgaaaaagcc cggcgttgcc gggctttgtt tttgcgttag ctgggcttgt 24120ttgacaggcc caagctctga ctgcgcccgc gctcgcgctc ctgggcctgt ttcttctcct 24180gctcctgctt gcgcatcagg gcctggtgcc gtcgggctgc ttcacgcatc gaatcccagt 24240cgccggccag ctcgggatgc tccgcgcgca tcttgcgcgt cgccagttcc tcgatcttgg 24300gcgcgtgaat gcccatgcct tccttgattt cgcgcaccat gtccagccgc gtgtgcaggg 24360tctgcaagcg ggcttgctgt tgggcctgct gctgctgcca ggcggccttt gtacgcggca 24420gggacagcaa gccgggggca ttggactgta gctgctgcaa acgcgcctgc tgacggtcta 24480cgagctgttc taggcggtcc tcgatgcgct ccacctggtc atgctttgcc tgcacgtaga 24540gcgcaagggt ctgctggtag gtctgctcga tgggcgcgga ttctaagagg gcctgctgtt 24600ccgtctcggc ctcctgggcc gcctgtagca aatcctcgcc gctgttgccg ctggactgct 24660ttactgccgg ggactgctgt tgccctgctc gcgccgtcgt cgcagttcgg cttgccccca 24720ctcgattgac tgcttcattt cgagccgcag cgatgcgatc tcggattgcg tcaacggacg 24780gggcagcgcg gaggtgtccg gcttctcctt gggtgagtcg gtcgatgcca tagccaaagg 24840tttccttcca aaatgcgtcc attgctggac cgtgtttctc attgatgccc gcaagcatct 24900tcggcttgac cgccaggtca agcgcgcctt catgggcggt catgacggac gccgccatga 24960ccttgccgcc gttgttctcg atgtagccgc gtaatgaggc aatggtgccg cccatcgtca 25020gcgtgtcatc gacaacgatg tacttctggc cggggatcac ctccccctcg aaagtcgggt 25080tgaacgccag gcgatgatct gaaccggctc cggttcgggc gaccttctcc cgctgcacaa 25140tgtccgtttc gacctcaagg ccaaggcggt cggccagaac gaccgccatc atggccggaa 25200tcttgttgtt ccccgccgcc tcgacggcga ggactggaac gatgcggggc ttgtcgtcgc 25260cgatcagcgt cttgagctgg gcaacagtgt cgtccgaaat caggcgctcg accaaattaa 25320gcgccgcttc cgcgtcgccc tgcttcgcag cctggtattc aggctcgttg gtcaaagaac 25380caaggtcgcc gttgcgaacc accttcggga agtctcccca cggtgcgcgc tcggctctgc 25440tgtagctgct caagacgcct ccctttttag ccgctaaaac tctaacgagt gcgcccgcga 25500ctcaacttga cgctttcggc acttacctgt gccttgccac ttgcgtcata ggtgatgctt 25560ttcgcactcc cgatttcagg tactttatcg aaatctgacc gggcgtgcat tacaaagttc 25620ttccccacct gttggtaaat gctgccgcta tctgcgtgga cgatgctgcc gtcgtggcgc 25680tgcgacttat cggccttttg ggccatatag atgttgtaaa tgccaggttt cagggccccg 25740gctttatcta ccttctggtt cgtccatgcg ccttggttct cggtctggac aattctttgc

25800ccattcatga ccaggaggcg gtgtttcatt gggtgactcc tgacggttgc ctctggtgtt 25860aaacgtgtcc tggtcgcttg ccggctaaaa aaaagccgac ctcggcagtt cgaggccggc 25920tttccctaga gccgggcgcg tcaaggttgt tccatctatt ttagtgaact gcgttcgatt 25980tatcagttac tttcctcccg ctttgtgttt cctcccactc gtttccgcgt ctagccgacc 26040cctcaacata gcggcctctt cttgggctgc ctttgcctct tgccgcgctt cgtcacgctc 26100ggcttgcacc gtcgtaaagc gctcggcctg cctggccgcc tcttgcgccg ccaacttcct 26160ttgctcctgg tgggcctcgg cgtcggcctg cgccttcgct ttcaccgctg ccaactccgt 26220gcgcaaactc tccgcttcgc gcctggtggc gtcgcgctcg ccgcgaagcg cctgcatttc 26280ctggttggcc gcgtccaggg tcttgcggct ctcttctttg aatgcgcggg cgtcctggtg 26340agcgtagtcc agctcggcgc gcagctcctg cgctcgacgc tccacctcgt cggcccgctg 26400cgtcgccagc gcggcccgct gctcggctcc tgccagggcg gtgcgtgctt cggccagggc 26460ttgccgctgg cgtgcggcca gctcggccgc ctcggcggcc tgctgctcta gcaatgtaac 26520gcgcgcctgg gcttcttcca gctcgcgggc ctgcgcctcg aaggcgtcgg ccagctcccc 26580gcgcacggct tccaactcgt tgcgctcacg atcccagccg gcttgcgctg cctgcaacga 26640ttcattggca agggcctggg cggcttgcca gagggcggcc acggcctggt tgccggcctg 26700ctgcaccgcg tccggcacct ggactgccag cggggcggcc tgcgccgtgc gctggcgtcg 26760ccattcgcgc atgccggcgc tggcgtcgtt catgttgacg cgggcggcct tacgcactgc 26820atccacggtc gggaagttct cccggtcgcc ttgctcgaac agctcgtccg cagccgcaaa 26880aatgcggtcg cgcgtctctt tgttcagttc catgttggct ccggtaattg gtaagaataa 26940taatactctt acctacctta tcagcgcaag agtttagctg aacagttctc gacttaacgg 27000caggtttttt agcggctgaa gggcaggcaa aaaaagcccc gcacggtcgg cgggggcaaa 27060gggtcagcgg gaaggggatt agcgggcgtc gggcttcttc atgcgtcggg gccgcgcttc 27120ttgggatgga gcacgacgaa gcgcgcacgc gcatcgtcct cggccctatc ggcccgcgtc 27180gcggtcagga acttgtcgcg cgctaggtcc tccctggtgg gcaccagggg catgaactcg 27240gcctgctcga tgtaggtcca ctccatgacc gcatcgcagt cgaggccgcg ttccttcacc 27300gtctcttgca ggtcgcggta cgcccgctcg ttgagcggct ggtaacgggc caattggtcg 27360taaatggctg tcggccatga gcggcctttc ctgttgagcc agcagccgac gacgaagccg 27420gcaatgcagg cccctggcac aaccaggccg acgccggggg caggggatgg cagcagctcg 27480ccaaccagga accccgccgc gatgatgccg atgccggtca accagccctt gaaactatcc 27540ggccccgaaa cacccctgcg cattgcctgg atgctgcgcc ggatagcttg caacatcagg 27600agccgtttct tttgttcgtc agtcatggtc cgccctcacc agttgttcgt atcggtgtcg 27660gacgaactga aatcgcaaga gctgccggta tcggtccagc cgctgtccgt gtcgctgctg 27720ccgaagcacg gcgaggggtc cgcgaacgcc gcagacggcg tatccggccg cagcgcatcg 27780cccagcatgg ccccggtcag cgagccgccg gccaggtagc ccagcatggt gctgttggtc 27840gccccggcca ccagggccga cgtgacgaaa tcgccgtcat tccctctgga ttgttcgctg 27900ctcggcgggg cagtgcgccg cgccggcggc gtcgtggatg gctcgggttg gctggcctgc 27960gacggccggc gaaaggtgcg cagcagctcg ttatcgaccg gctgcggcgt cggggccgcc 28020gccttgcgct gcggtcggtg ttccttcttc ggctcgcgca gcttgaacag catgatcgcg 28080gaaaccagca gcaacgccgc gcctacgcct cccgcgatgt agaacagcat cggattcatt 28140cttcggtcct ccttgtagcg gaaccgttgt ctgtgcggcg cgggtggccc gcgccgctgt 28200ctttggggat cagccctcga tgagcgcgac cagtttcacg tcggcaaggt tcgcctcgaa 28260ctcctggccg tcgtcctcgt acttcaacca ggcatagcct tccgccggcg gccgacggtt 28320gaggataagg cgggcagggc gctcgtcgtg ctcgacctgg acgatggcct ttttcagctt 28380gtccgggtcc ggctccttcg cgcccttttc cttggcgtcc ttaccgtcct ggtcgccgtc 28440ctcgccgtcc tggccgtcgc cggcctccgc gtcacgctcg gcatcagtct ggccgttgaa 28500ggcatcgacg gtgttgggat cgcggccctt ctcgtccagg aactcgcgca gcagcttgac 28560cgtgccgcgc gtgatttcct gggtgtcgtc gtcaagccac gcctcgactt cctccgggcg 28620cttcttgaag gccgtcacca gctcgttcac cacggtcacg tcgcgcacgc ggccggtgtt 28680gaacgcatcg gcgatcttct ccggcaggtc cagcagcgtg acgtgctggg tgatgaacgc 28740cggcgacttg ccgatttcct tggcgatatc gcctttcttc ttgcccttcg ccagctcgcg 28800gccaatgaag tcggcaattt cgcgcggggt cagctcgttg cgttgcaggt tctcgataac 28860ctggtcggct tcgttgtagt cgttgtcgat gaacgccggg atggacttct tgccggccca 28920cttcgagcca cggtagcggc gggcgccgtg attgatgata tagcggcccg gctgctcctg 28980gttctcgcgc accgaaatgg gtgacttcac cccgcgctct ttgatcgtgg caccgatttc 29040cgcgatgctc tccggggaaa agccggggtt gtcggccgtc cgcggctgat gcggatcttc 29100gtcgatcagg tccaggtcca gctcgatagg gccggaaccg ccctgagacg ccgcaggagc 29160gtccaggagg ctcgacaggt cgccgatgct atccaacccc aggccggacg gctgcgccgc 29220gcctgcggct tcctgagcgg ccgcagcggt gtttttcttg gtggtcttgg cttgagccgc 29280agtcattggg aaatctccat cttcgtgaac acgtaatcag ccagggcgcg aacctctttc 29340gatgccttgc gcgcggccgt tttcttgatc ttccagaccg gcacaccgga tgcgagggca 29400tcggcgatgc tgctgcgcag gccaacggtg gccggaatca tcatcttggg gtacgcggcc 29460agcagctcgg cttggtggcg cgcgtggcgc ggattccgcg catcgacctt gctgggcacc 29520atgccaagga attgcagctt ggcgttcttc tggcgcacgt tcgcaatggt cgtgaccatc 29580ttcttgatgc cctggatgct gtacgcctca agctcgatgg gggacagcac atagtcggcc 29640gcgaagaggg cggccgccag gccgacgcca agggtcgggg ccgtgtcgat caggcacacg 29700tcgaagcctt ggttcgccag ggccttgatg ttcgccccga acagctcgcg ggcgtcgtcc 29760agcgacagcc gttcggcgtt cgccagtacc gggttggact cgatgagggc gaggcgcgcg 29820gcctggccgt cgccggctgc gggtgcggtt tcggtccagc cgccggcagg gacagcgccg 29880aacagcttgc ttgcatgcag gccggtagca aagtccttga gcgtgtagga cgcattgccc 29940tgggggtcca ggtcgatcac ggcaacccgc aagccgcgct cgaaaaagtc gaaggcaaga 30000tgcacaaggg tcgaagtctt gccgacgccg cctttctggt tggccgtgac caaagttttc 30060atcgtttggt ttcctgtttt ttcttggcgt ccgcttccca cttccggacg atgtacgcct 30120gatgttccgg cagaaccgcc gttacccgcg cgtacccctc gggcaagttc ttgtcctcga 30180acgcggccca cacgcgatgc accgcttgcg acactgcgcc cctggtcagt cccagcgacg 30240ttgcgaacgt cgcctgtggc ttcccatcga ctaagacgcc ccgcgctatc tcgatggtct 30300gctgccccac ttccagcccc tggatcgcct cctggaactg gctttcggta agccgtttct 30360tcatggataa cacccataat ttgctccgcg ccttggttga acatagcggt gacagccgcc 30420agcacatgag agaagtttag ctaaacattt ctcgcacgtc aacaccttta gccgctaaaa 30480ctcgtccttg gcgtaacaaa acaaaagccc ggaaaccggg ctttcgtctc ttgccgctta 30540tggctctgca cccggctcca tcaccaacag gtcgcgcacg cgcttcactc ggttgcggat 30600cgacactgcc agcccaacaa agccggttgc cgccgccgcc aggatcgcgc cgatgatgcc 30660ggccacaccg gccatcgccc accaggtcgc cgccttccgg ttccattcct gctggtactg 30720cttcgcaatg ctggacctcg gctcaccata ggctgaccgc tcgatggcgt atgccgcttc 30780tccccttggc gtaaaaccca gcgccgcagg cggcattgcc atgctgcccg ccgctttccc 30840gaccacgacg cgcgcaccag gcttgcggtc cagaccttcg gccacggcga gctgcgcaag 30900gacataatca gccgccgact tggctccacg cgcctcgatc agctcttgca ctcgcgcgaa 30960atccttggcc tccacggccg ccatgaatcg cgcacgcggc gaaggctccg cagggccggc 31020gtcgtgatcg ccgccgagaa tgcccttcac caagttcgac gacacgaaaa tcatgctgac 31080ggctatcacc atcatgcaga cggatcgcac gaacccgctg aattgaacac gagcacggca 31140cccgcgacca ctatgccaag aatgcccaag gtaaaaattg ccggccccgc catgaagtcc 31200gtgaatgccc cgacggccga agtgaagggc aggccgccac ccaggccgcc gccctcactg 31260cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat gcttccgggc 31320gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga tcccggcaat ggcaaggact 31380gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg 31440gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg 31500cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg 31560tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca 31620aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct 31680gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc 31740cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca tctgtgggaa 31800actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg 31860ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt 31920gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt atccacaacg 31980ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc 32040atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg gaaaggcgct 32100ggaagccccg tagcgacgcg gagaggggcg agacaagcca agggcgcagg ctcgatgcgc 32160agcacgacat agccggttct cgcaaggacg agaatttccc tgcggtgccc ctcaagtgtc 32220aatgaaagtt tccaacgcga gccattcgcg agagccttga gtccacgcta gatgagagct 32280ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga acggtctgcg 32340ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa 32400agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca 32460tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt 32520caacgggaaa cgtcttgctc gactctagag ctcgttcctc gaggcctcga ggcctcgagg 32580aacggtacct gcggggaagc ttacaataat gtgtgttgtt aagtcttgtt gcctgtcatc 32640gtctgactga ctttcgtcat aaatcccggc ctccgtaacc cagctttggg caagctcacg 32700gatttgatcc ggcggaacgg gaatatcgag atgccgggct gaacgctgca gttccagctt 32760tccctttcgg gacaggtact ccagctgatt gattatctgc tgaagggtct tggttccacc 32820tcctggcaca atgcgaatga ttacttgagc gcgatcgggc atccaatttt ctcccgtcag 32880gtgcgtggtc aagtgctaca aggcaccttt cagtaacgag cgaccgtcga tccgtcgccg 32940ggatacggac aaaatggagc gcagtagtcc atcgagggcg gcgaaagcct cgccaaaagc 33000aatacgttca tctcgcacag cctccagatc cgatcgaggg tcttcggcgt aggcagatag 33060aagcatggat acattgcttg agagtattcc gatggactga agtatggctt ccatcttttc 33120tcgtgtgtct gcatctattt cgagaaagcc cccgatgcgg cgcaccgcaa cgcgaattgc 33180catactatcc gaaagtccca gcaggcgcgc ttgataggaa aaggtttcat actcggccga 33240tcgcagacgg gcactcacga ccttgaaccc ttcaactttc agggatcgat gctggttgat 33300ggtagtctca ctcgacgtgg ctctggtgtg ttttgacata gcttcctcca aagaaagcgg 33360aaggtctgga tactccagca cgaaatgtgc ccgggtagac ggatggaagt ctagccctgc 33420tcaatatgaa atcaacagta catttacagt caatactgaa tatacttgct acatttgcaa 33480ttgtcttata acgaatgtga aataaaaata gtgtaacaac gcttttactc atcgataatc 33540acaaaaacat ttatacgaac aaaaatacaa atgcactccg gtttcacagg ataggcggga 33600tcagaatatg caacttttga cgttttgttc tttcaaaggg ggtgctggca aaaccaccgc 33660actcatgggc ctttgcgctg ctttggcaaa tgacggtaaa cgagtggccc tctttgatgc 33720cgacgaaaac cggcctctga cgcgatggag agaaaacgcc ttacaaagca gtactgggat 33780cctcgctgtg aagtctattc cgccgacgaa atgccccttc ttgaagcagc ctatgaaaat 33840gccgagctcg aaggatttga ttatgcgttg gccgatacgc gtggcggctc gagcgagctc 33900aacaacacaa tcatcgctag ctcaaacctg cttctgatcc ccaccatgct aacgccgctc 33960gacatcgatg aggcactatc tacctaccgc tacgtcatcg agctgctgtt gagtgaaaat 34020ttggcaattc ctacagctgt tttgcgccaa cgcgtcccgg tcggccgatt gacaacatcg 34080caacgcagga tgtcagagac gctagagagc cttccagttg taccgtctcc catgcatgaa 34140agagatgcat ttgccgcgat gaaagaacgc ggcatgttgc atcttacatt actaaacacg 34200ggaactgatc cgacgatgcg cctcatagag aggaatcttc ggattgcgat ggaggaagtc 34260gtggtcattt cgaaactgat cagcaaaatc ttggaggctt gaagatggca attcgcaagc 34320ccgcattgtc ggtcggcgaa gcacggcggc ttgctggtgc tcgacccgag atccaccatc 34380ccaacccgac acttgttccc cagaagctgg acctccagca cttgcctgaa aaagccgacg 34440agaaagacca gcaacgtgag cctctcgtcg ccgatcacat ttacagtccc gatcgacaac 34500ttaagctaac tgtggatgcc cttagtccac ctccgtcccc gaaaaagctc caggtttttc 34560tttcagcgcg accgcccgcg cctcaagtgt cgaaaacata tgacaacctc gttcggcaat 34620acagtccctc gaagtcgcta caaatgattt taaggcgcgc gttggacgat ttcgaaagca 34680tgctggcaga tggatcattt cgcgtggccc cgaaaagtta tccgatccct tcaactacag 34740aaaaatccgt tctcgttcag acctcacgca tgttcccggt tgcgttgctc gaggtcgctc 34800gaagtcattt tgatccgttg gggttggaga ccgctcgagc tttcggccac aagctggcta 34860ccgccgcgct cgcgtcattc tttgctggag agaagccatc gagcaattgg tgaagaggga 34920cctatcggaa cccctcacca aatattgagt gtaggtttga ggccgctggc cgcgtcctca 34980gtcacctttt gagccagata attaagagcc aaatgcaatt ggctcaggct gccatcgtcc 35040ccccgtgcga aacctgcacg tccgcgtcaa agaaataacc ggcacctctt gctgttttta 35100tcagttgagg gcttgacgga tccgcctcaa gtttgcggcg cagccgcaaa atgagaacat 35160ctatactcct gtcgtaaacc tcctcgtcgc gtactcgact ggcaatgaga agttgctcgc 35220gcgatagaac gtcgcggggt ttctctaaaa acgcgaggag aagattgaac tcacctgccg 35280taagtttcac ctcaccgcca gcttcggaca tcaagcgacg ttgcctgaga ttaagtgtcc 35340agtcagtaaa acaaaaagac cgtcggtctt tggagcggac aacgttgggg cgcacgcgca 35400aggcaacccg aatgcgtgca agaaactctc tcgtactaaa cggcttagcg ataaaatcac 35460ttgctcctag ctcgagtgca acaactttat ccgtctcctc aaggcggtcg ccactgataa 35520ttatgattgg aatatcagac tttgccgcca gatttcgaac gatctcaagc ccatcttcac 35580gacctaaatt tagatcaaca accacgacat cgaccgtcgc ggaagagagt actctagtga 35640actgggtgct gtcggctacc gcggtcactt tgaaggcgtg gatcgtaagg tattcgataa 35700taagatgccg catagcgaca tcgtcatcga taagaagaac gtgtttcaac ggctcacctt 35760tcaatctaaa atctgaaccc ttgttcacag cgcttgagaa attttcacgt gaaggatgta 35820caatcatctc cagctaaatg ggcagttcgt cagaattgcg gctgaccgcg gatgacgaaa 35880atgcgaacca agtatttcaa ttttatgaca aaagttctca atcgttgtta caagtgaaac 35940gcttcgaggt tacagctact attgattaag gagatcgcct atggtctcgc cccggcgtcg 36000tgcgtccgcc gcgagccaga tctcgcctac ttcataaacg tcctcatagg cacggaatgg 36060aatgatgaca tcgatcgccg tagagagcat gtcaatcagt gtgcgatctt ccaagctagc 36120accttgggcg ctacttttga caagggaaaa cagtttcttg aatccttgga ttggattcgc 36180gccgtgtatt gttgaaatcg atcccggatg tcccgagacg acttcactca gataagccca 36240tgctgcatcg tcgcgcatct cgccaagcaa tatccggtcc ggccgcatac gcagacttgc 36300ttggagcaag tgctcggcgc tcacagcacc cagcccagca ccgttcttgg agtagagtag 36360tctaacatga ttatcgtgtg gaatgacgag ttcgagcgta tcttctatgg tgattagcct 36420ttcctggggg gggatggcgc tgatcaaggt cttgctcatt gttgtcttgc cgcttccggt 36480agggccacat agcaacatcg tcagtcggct gacgacgcat gcgtgcagaa acgcttccaa 36540atccccgttg tcaaaatgct gaaggatagc ttcatcatcc tgattttggc gtttccttcg 36600tgtctgccac tggttccacc tcgaagcatc ataacgggag gagacttctt taagaccaga 36660aacacgcgag cttggccgtc gaatggtcaa gctgacggtg cccgagggaa cggtcggcgg 36720cagacagatt tgtagtcgtt caccaccagg aagttcagtg gcgcagaggg ggttacgtgg 36780tccgacatcc tgctttctca gcgcgcccgc taaaatagcg atatcttcaa gatcatcata 36840agagacgggc aaaggcatct tggtaaaaat gccggcttgg cgcacaaatg cctctccagg 36900tcgattgatc gcaatttctt cagtcttcgg gtcatcgagc cattccaaaa tcggcttcag 36960aagaaagcgt agttgcggat ccacttccat ttacaatgta tcctatctct aagcggaaat 37020ttgaattcat taagagcggc ggttcctccc ccgcgtggcg ccgccagtca ggcggagctg 37080gtaaacacca aagaaatcga ggtcccgtgc tacgaaaatg gaaacggtgt caccctgatt 37140cttcttcagg gttggcggta tgttgatggt tgccttaagg gctgtctcag ttgtctgctc 37200accgttattt tgaaagctgt tgaagctcat cccgccaccc gagctgccgg cgtaggtgct 37260agctgcctgg aaggcgcctt gaacaacact caagagcata gctccgctaa aacgctgcca 37320gaagtggctg tcgaccgagc ccggcaatcc tgagcgaccg agttcgtccg cgcttggcga 37380tgttaacgag atcatcgcat ggtcaggtgt ctcggcgcga tcccacaaca caaaaacgcg 37440cccatctccc tgttgcaagc cacgctgtat ttcgccaaca acggtggtgc cacgatcaag 37500aagcacgata ttgttcgttg ttccacgaat atcctgaggc aagacacact ttacatagcc 37560tgccaaattt gtgtcgattg cggtttgcaa gatgcacgga attattgtcc cttgcgttac 37620cataaaatcg gggtgcggca agagcgtggc gctgctgggc tgcagctcgg tgggtttcat 37680acgtatcgac aaatcgttct cgccggacac ttcgccattc ggcaaggagt tgtcgtcacg 37740cttgccttct tgtcttcggc ccgtgtcgcc ctgaatggcg cgtttgctga ccccttgatc 37800gccgctgcta tatgcaaaaa tcggtgtttc ttccggccgt ggctcatgcc gctccggttc 37860gcccctcggc ggtagaggag cagcaggctg aacagcctct tgaaccgctg gaggatccgg 37920cggcacctca atcggagctg gatgaaatgg cttggtgttt gttgcgatca aagttgacgg 37980cgatgcgttc tcattcacct tcttttggcg cccacctagc caaatgaggc ttaatgataa 38040cgcgagaacg acacctccga cgatcaattt ctgagacccc gaaagacgcc ggcgatgttt 38100gtcggagacc agggatccag atgcatcaac ctcatgtgcc gcttgctgac tatcgttatt 38160catcccttcg cccccttcag gacgcgtttc acatcgggcc tcaccgtgcc cgtttgcggc 38220ctttggccaa cgggatcgta agcggtgttc cagatacata gtactgtgtg gccatccctc 38280agacgccaac ctcgggaaac cgaagaaatc tcgacatcgc tccctttaac tgaatagttg 38340gcaacagctt ccttgccatc aggattgatg gtgtagatgg agggtatgcg tacattgccc 38400ggaaagtgga ataccgtcgt aaatccattg tcgaagactt cgagtggcaa cagcgaacga 38460tcgccttggg cgacgtagtg ccaattactg tccgccgcac caagggctgt gacaggctga 38520tccaataaat tctcagcttt ccgttgatat tgtgcttccg cgtgtagtct gtccacaaca 38580gccttctgtt gtgcctccct tcgccgagcc gccgcatcgt cggcggggta ggcgaattgg 38640acgctgtaat agagatcggg ctgctcttta tcgaggtggg acagagtctt ggaacttata 38700ctgaaaacat aacggcgcat cccggagtcg cttgcggtta gcacgattac tggctgaggc 38760gtgaggacct ggcttgcctt gaaaaataga taatttcccc gcggtagggc tgctagatct 38820ttgctatttg aaacggcaac cgctgtcacc gtttcgttcg tggcgaatgt tacgaccaaa 38880gtagctccaa ccgccgtcga gaggcgcacc acttgatcgg gattgtaagc caaataacgc 38940atgcgcggat ctagcttgcc cgccattgga gtgtcttcag cctccgcacc agtcgcagcg 39000gcaaataaac atgctaaaat gaaaagtgct tttctgatca tggttcgctg tggcctacgt 39060ttgaaacggt atcttccgat gtctgatagg aggtgacaac cagacctgcc gggttggtta 39120gtctcaatct gccgggcaag ctggtcacct tttcgtagcg aactgtcgcg gtccacgtac 39180tcaccacagg cattttgccg tcaacgacga gggtcctttt atagcgaatt tgctgcgtgc 39240ttggagttac atcatttgaa gcgatgtgct cgacctccac cctgccgcgt ttgccaagaa 39300tgacttgagg cgaactggga ttgggatagt tgaagaattg ctggtaatcc tggcgcactg 39360ttggggcact gaagttcgat accaggtcgt aggcgtactg agcggtgtcg gcatcataac 39420tctcgcgcag gcgaacgtac tcccacaatg aggcgttaac gacggcctcc tcttgagttg 39480caggcaatcg cgagacagac acctcgctgt caacggtgcc gtccggccgt atccatagat 39540atacgggcac aagcctgctc aacggcacca ttgtggctat agcgaacgct tgagcaacat 39600ttcccaaaat cgcgatagct gcgacagctg caatgagttt ggagagacgt cgcgccgatt 39660tcgctcgcgc ggtttgaaag gcttctactt ccttatagtg ctcggcaagg ctttcgcgcg 39720ccactagcat ggcatattca ggccccgtca tagcgtccac ccgaattgcc gagctgaaga 39780tctgacggag taggctgcca tcgccccaca ttcagcggga agatcgggcc tttgcagctc 39840gctaatgtgt cgtttgtctg gcagccgctc aaagcgacaa ctaggcacag caggcaatac 39900ttcatagaat tctccattga ggcgaatttt tgcgcgacct agcctcgctc aacctgagcg 39960aagcgacggt acaagctgct ggcagattgg gttgcgccgc tccagtaact gcctccaatg 40020ttgccggcga tcgccggcaa agcgacaatg agcgcatccc ctgtcagaaa aaacatatcg 40080agttcgtaaa gaccaatgat cttggccgcg gtcgtaccgg cgaaggtgat tacaccaagc 40140ataagggtga gcgcagtcgc ttcggttagg atgacgatcg ttgccacgag gtttaagagg 40200agaagcaaga gaccgtaggt gataagttgc ccgatccact tagctgcgat gtcccgcgtg 40260cgatcaaaaa tatatccgac gaggatcaga ggcccgatcg cgagaagcac tttcgtgaga 40320attccaacgg cgtcgtaaac tccgaaggca gaccagagcg tgccgtaaag gacccactgt 40380gccccttgga aagcaaggat gtcctggtcg ttcatcggac cgatttcgga tgcgattttc 40440tgaaaaacgg cctgggtcac ggcgaacatt gtatccaact gtgccggaac agtctgcaga 40500ggcaagccgg ttacactaaa ctgctgaaca aagtttggga ccgtcttttc gaagatggaa 40560accacatagt cttggtagtt agcctgccca acaattagag caacaacgat ggtgaccgtg 40620atcacccgag tgataccgct acgggtatcg acttcgccgc gtatgactaa aataccctga 40680acaataatcc aaagagtgac acaggcgatc aatggcgcac tcaccgcctc ctggatagtc 40740tcaagcatcg agtccaagcc tgtcgtgaag gctacatcga agatcgtatg aatggccgta 40800aacggcgccg gaatcgtgaa attcatcgat tggacctgaa cttgactggt ttgtcgcata

40860atgttggata aaatgagctc gcattcggcg aggatgcggg cggatgaaca aatcgcccag 40920ccttagggga gggcaccaaa gatgacagcg gtcttttgat gctccttgcg ttgagcggcc 40980gcctcttccg cctcgtgaag gccggcctgc gcggtagtca tcgttaatag gcttgtcgcc 41040tgtacatttt gaatcattgc gtcatggatc tgcttgagaa gcaaaccatt ggtcacggtt 41100gcctgcatga tattgcgaga tcgggaaagc tgagcagacg tatcagcatt cgccgtcaag 41160cgtttgtcca tcgtttccag attgtcagcc gcaatgccag cgctgtttgc ggaaccggtg 41220atctgcgatc gcaacaggtc cgcttcagca tcactaccca cgactgcacg atctgtatcg 41280ctggtgatcg cacgtgccgt ggtcgacatt ggcattcgcg gcgaaaacat ttcattgtct 41340aggtccttcg tcgaaggata ctgatttttc tggttgagcg aagtcagtag tccagtaacg 41400ccgtaggccg acgtcaacat cgtaaccatc gctatagtct gagtgagatt ctccgcagtc 41460gcgagcgcag tcgcgagcgt ctcagcctcc gttgccgggt cgctaacaac aaactgcgcc 41520cgcgcgggct gaatatatag aaagctgcag gtcaaaactg ttgcaataag ttgcgtcgtc 41580ttcatcgttt cctaccttat caatcttctg cctcgtggtg acgggccatg aattcgctga 41640gccagccaga tgagttgcct tcttgtgcct cgcgtagtcg agttgcaaag cgcaccgtgt 41700tggcacgccc cgaaagcacg gcgacatatt cacgcatatc ccgcagatca aattcgcaga 41760tgacgcttcc actttctcgt ttaagaagaa acttacggct gccgaccgtc atgtcttcac 41820ggatcgcctg aaattccttt tcggtacatt tcagtccatc gacataagcc gatcgatctg 41880cggttggtga tggatagaaa atcttcgtca tacattgcgc aaccaagctg gctcctagcg 41940gcgattccag aacatgctct ggttgctgcg ttgccagtat tagcatcccg ttgttttttc 42000gaacggtcag gaggaatttg tcgacgacag tcgaaaattt agggtttaac aaataggcgc 42060gaaactcatc gcagctcatc acaaaacggc ggccgtcgat catggctcca atccgatgca 42120ggagatatgc tgcagcggga gcgcatactt cctcgtattc gagaagatgc gtcatgtcga 42180agccggtaat cgacggatct aactttactt cgtcaacttc gccgtcaaat gcccagccaa 42240gcgcatggcc ccggcaccag cgttggagcc gcgctcctgc gccttcggcg ggcccatgca 42300acaaaaattc acgtaacccc gcgattgaac gcatttgtgg atcaaacgag agctgacgat 42360ggataccacg gaccagacgg cggttctctt ccggagaaat cccaccccga ccatcactct 42420cgatgagagc cacgatccat tcgcgcagaa aatcgtgtga ggctgctgtg ttttctaggc 42480cacgcaacgg cgccaacccg ctgggtgtgc ctctgtgaag tgccaaatat gttcctcctg 42540tggcgcgaac cagcaattcg ccaccccggt ccttgtcaaa gaacacgacc gtacctgcac 42600ggtcgaccat gctctgttcg agcatggcta gaacaaacat catgagcgtc gtcttacccc 42660tcccgatagg cccgaatatt gccgtcatgc caacatcgtg ctcatgcggg atatagtcga 42720aaggcgttcc gccattggta cgaaatcggg caatcgcgtt gccccagtgg cctgagctgg 42780cgccctctgg aaagttttcg aaagagacaa accctgcgaa attgcgtgaa gtgattgcgc 42840cagggcgtgt gcgccactta aaattccccg gcaattggga ccaataggcc gcttccatac 42900caataccttc ttggacaacc acggcacctg catccgccat tcgtgtccga gcccgcgcgc 42960ccctgtcccc aagactattg agatcgtctg catagacgca aaggctcaaa tgatgtgagc 43020ccataacgaa ttcgttgctc gcaagtgcgt cctcagcctc ggataatttg ccgatttgag 43080tcacggcttt atcgccggaa ctcagcatct ggctcgattt gaggctaagt ttcgcgtgcg 43140cttgcgggcg agtcaggaac gaaaaactct gcgtgagaac aagtggaaaa tcgagggata 43200gcagcgcgtt gagcatgccc ggccgtgttt ttgcagggta ttcgcgaaac gaatagatgg 43260atccaacgta actgtctttt ggcgttctga tctcgagtcc tcgcttgccg caaatgactc 43320tgtcggtata aatcgaagcg ccgagtgagc cgctgacgac cggaaccggt gtgaaccgac 43380cagtcatgat caaccgtagc gcttcgccaa tttcggtgaa gagcacaccc tgcttctcgc 43440ggatgccaag acgatgcagg ccatacgctt taagagagcc agcgacaaca tgccaaagat 43500cttccatgtt cctgatctgg cccgtgagat cgttttccct ttttccgctt agcttggtga 43560acctcctctt taccttccct aaagccgcct gtgggtagac aatcaacgta aggaagtgtt 43620cattgcggag gagttggccg gagagcacgc gctgttcaaa agcttcgttc aggctagcgg 43680cgaaaacact acggaagtgt cgcggcgccg atgatggcac gtcggcatga cgtacgaggt 43740gagcatatat tgacacatga tcatcagcga tattgcgcaa cagcgtgttg aacgcacgac 43800aacgcgcatt gcgcatttca gtttcctcaa gctcgaatgc aacgccatca attctcgcaa 43860tggtcatgat cgatccgtct tcaagaagga cgatatggtc gctgaggtgg ccaatataag 43920ggagatagat ctcaccggat ctttcggtcg ttccactcgc gccgagcatc acaccattcc 43980tctccctcgt gggggaaccc taattggatt tgggctaaca gtagcgcccc cccaaactgc 44040actatcaatg cttcttcccg cggtccgcaa aaatagcagg acgacgctcg ccgcattgta 44100gtctcgctcc acgatgagcc gggctgcaaa ccataacggc acgagaacga cttcgtagag 44160cgggttctga acgataacga tgacaaagcc ggcgaacatc atgaataacc ctgccaatgt 44220cagtggcacc ccaagaaaca atgcgggccg tgtggctgcg aggtaaaggg tcgattcttc 44280caaacgatca gccatcaact accgccagtg agcgtttggc cgaggaagct cgccccaaac 44340atgataacaa tgccgccgac gacgccggca accagcccaa gcgaagcccg cccgaacatc 44400caggagatcc cgatagcgac aatgccgaga acagcgagtg actggccgaa cggaccaagg 44460ataaacgtgc atatattgtt aaccattgtg gcggggtcag tgccgccacc cgcagattgc 44520gctgcggcgg gtccggatga ggaaatgctc catgcaattg caccgcacaa gcttggggcg 44580cagctcgata tcacgcgcat catcgcattc gagagcgaga ggcgatttag atgtaaacgg 44640tatctctcaa agcatcgcat caatgcgcac ctccttagta taagtcgaat aagacttgat 44700tgtcgtctgc ggatttgccg ttgtcctggt gtggcggtgg cggagcgatt aaaccgccag 44760cgccatcctc ctgcgagcgg cgctgatatg acccccaaac atcccacgtc tcttcggatt 44820ttagcgcctc gtgatcgtct tttggaggct cgattaacgc gggcaccagc gattgagcag 44880ctgtttcaac ttttcgcacg tagccgtttg caaaaccgcc gatgaaatta ccggtgttgt 44940aagcggagat cgcccgacga agcgcaaatt gcttctcgtc aatcgtttcg ccgcctgcat 45000aacgactttt cagcatgttt gcagcggcag ataatgatgt gcacgcctgg agcgcaccgt 45060caggtgtcag accgagcata gaaaaatttc gagagtttat ttgcatgagg ccaacatcca 45120gcgaatgccg tgcatcgaga cggtgcctga cgacttgggt tgcttggctg tgatcttgcc 45180agtgaagcgt ttcgccggtc gtgttgtcat gaatcgctaa aggatcaaag cgactctcca 45240ccttagctat cgccgcaagc gtagatgtcg caactgatgg ggcacacttg cgagcaacat 45300ggtcaaactc agcagatgag agtggcgtgg caaggctcga cgaacagaag gagaccatca 45360aggcaagaga aagcgacccc gatctcttaa gcatacctta tctccttagc tcgcaactaa 45420caccgcctct cccgttggaa gaagtgcgtt gttttatgtt gaagattatc gggagggtcg 45480gttactcgaa aattttcaat tgcttcttta tgatttcaat tgaagcgaga aacctcgccc 45540ggcgtcttgg aacgcaacat ggaccgagaa ccgcgcatcc atgactaagc aaccggatcg 45600acctattcag gccgcagttg gtcaggtcag gctcagaacg aaaatgctcg gcgaggttac 45660gctgtctgta aacccattcg atgaacggga agcttccttc cgattgctct tggcaggaat 45720attggcccat gcctgcttgc gctttgcaaa tgctcttatc gcgttggtat catatgcctt 45780gtccgccagc agaaacgcac tctaagcgat tatttgtaaa aatgtttcgg tcatgcggcg 45840gtcatgggct tgacccgctg tcagcgcaag acggatcggt caaccgtcgg catcgacaac 45900agcgtgaatc ttggtggtca aaccgccacg ggaacgtccc atacagccat cgtcttgatc 45960ccgctgtttc ccgtcgccgc atgttggtgg acgcggacac aggaactgtc aatcatgacg 46020acattctatc gaaagccttg gaaatcacac tcagaatatg atcccagacg tctgcctcac 46080gccatcgtac aaagcgattg tagcaggttg tacaggaacc gtatcgatca ggaacgtctg 46140cccagggcgg gcccgtccgg aagcgccaca agatgacatt gatcacccgc gtcaacgcgc 46200ggcacgcgac gcggcttatt tgggaacaaa ggactgaaca acagtccatt cgaaatcggt 46260gacatcaaag cggggacggg ttatcagtgg cctccaagtc aagcctcaat gaatcaaaat 46320cagaccgatt tgcaaacctg atttatgagt gtgcggccta aatgatgaaa tcgtccttct 46380agatcgcctc cgtggtgtag caacacctcg cagtatcgcc gtgctgacct tggccaggga 46440attgactggc aagggtgctt tcacatgacc gctcttttgg ccgcgataga tgatttcgtt 46500gctgctttgg gcacgtagaa ggagagaagt catatcggag aaattcctcc tggcgcgaga 46560gcctgctcta tcgcgacggc atcccactgt cgggaacaga ccggatcatt cacgaggcga 46620aagtcgtcaa cacatgcgtt ataggcatct tcccttgaag gatgatcttg ttgctgccaa 46680tctggaggtg cggcagccgc aggcagatgc gatctcagcg caacttgcgg caaaacatct 46740cactcacctg aaaaccacta gcgagtctcg cgatcagacg aaggcctttt acttaacgac 46800acaatatccg atgtctgcat cacaggcgtc gctatcccag tcaatactaa agcggtgcag 46860gaactaaaga ttactgatga cttaggcgtg ccacgaggcc tgagacgacg cgcgtagaca 46920gttttttgaa atcattatca aagtgatggc ctccgctgaa gcctatcacc tctgcgccgg 46980tctgtcggag agatgggcaa gcattattac ggtcttcgcg cccgtacatg cattggacga 47040ttgcagggtc aatggatctg agatcatcca gaggattgcc gcccttacct tccgtttcga 47100gttggagcca gcccctaaat gagacgacat agtcgacttg atgtgacaat gccaagagag 47160agatttgctt aacccgattt ttttgctcaa gcgtaagcct attgaagctt gccggcatga 47220cgtccgcgcc gaaagaatat cctacaagta aaacattctg cacaccgaaa tgcttggtgt 47280agacatcgat tatgtgacca agatccttag cagtttcgct tggggaccgc tccgaccaga 47340aataccgaag tgaactgacg ccaatgacag gaatcccttc cgtctgcaga taggtaccat 47400cgatagatct gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 47460ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 47520ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 47580agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 47640atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 47700ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 47760ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 47820tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 47880tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 47940gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 48000ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 48060tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 48120agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 48180atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 48240acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 48300actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 48360tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 48420tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 48480tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 48540tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 48600caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 48660cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 48720agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 48780acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 48840gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 48900ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcagggg 48960gggggggggg gggggacttc cattgttcat tccacggaca aaaacagaga aaggaaacga 49020cagaggccaa aaagcctcgc tttcagcacc tgtcgtttcc tttcttttca gagggtattt 49080taaataaaaa cattaagtta tgacgaagaa gaacggaaac gccttaaacc ggaaaatttt 49140cataaatagc gaaaacccgc gaggtcgccg ccccgtaacc tgtcggatca ccggaaagga 49200cccgtaaagt gataatgatt atcatctaca tatcacaacg tgcgtggagg ccatcaaacc 49260acgtcaaata atcaattatg acgcaggtat cgtattaatt gatctgcatc aacttaacgt 49320aaaaacaact tcagacaata caaatcagcg acactgaata cggggcaacc tcatgtcccc 49380cccccccccc cccctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 49440agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 49500gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 49560atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 49620gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 49680tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 49740atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 49800agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 49860gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 49920cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 49980tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 50040ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 50100ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ggtcgacgat 50160cttgctgcgt tcggatattt tcgtggagtt cccgccacag acccggattg aaggcgagat 50220ccagcaactc gcgccagatc atcctgtgac ggaactttgg cgcgtgatga ctggccagga 50280cgtcggccga aagagcgaca agcagatcac gcttttcgac agcgtcggat ttgcgatcga 50340ggatttttcg gcgctgcgct acgtccgcga ccgcgttgag ggatcaagcc acagcagccc 50400actcgacctt ctagccgacc cagacgagcc aagggatctt tttggaatgc tgctccgtcg 50460tcaggctttc cgacgtttgg gtggttgaac agaagtcatt atcgtacgga atgccaagca 50520ctcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt 50580tcacgccctt ttaaatatcc gttattctaa taaacgctct tttctcttag gtttacccgc 50640caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatca 50700tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt 50760ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagcaag ctggtacgat 50820tgtaatacga ctcactatag ggcgaattga gcgctgttta aacgctcttc aactggaaga 50880gcggttacta ccggttaagt gactagggtc 50910650751DNAArtificial SequencePINII terminator control vector 6acgtgaccct agtcacttag gttaccagag ctggtcacct ttgtccacca agatggaact 60gcggccgctc attaattaag tcaggcgcgc ctctagttga agacacgttc atgtcttcat 120cgtaagaaga cactcagtag tcttcggcca gaatggccat ctggattcag caggcctaga 180aggccattta aatcctgagg atctggtctt cctaaggacc cgggatatcg ctatcaactt 240tgtatagaaa agttgggccg aattcgagct cggtacggcc agaatggccc ggaccgggtt 300accgaattcg agctcggtac cactagtaag cttgccgcaa ttcgcaaaac acacctagac 360tagatttgtt ttgctaaccc aattgatatt aattatatat gattaatatt tatatgtata 420tggatttggt taatgaaatg catctggttc atcaaagaat tataaagaca cgtgacattc 480atttaggata agaaatatgg atgatctctt tctcttttat tcagataact agtaattaca 540cataacacac aactttgatg cccacattat agtgattagc atgtcactat gtgtgcatcc 600ttttatttca tacattaatt aagttggcca atccagaaga tggacaagtc tggatcttca 660ttgtttgcct ccctgctgcg gtttttcacc gaagttcatg ccagtccagc gtttttgcag 720cagaaaagcc gccgacttcg gtttgcggtc gcgagtgaag atccctttct tgttaccgcc 780aacgcgcaat atgccttgcg aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac 840gacggcgctg acgcgatcaa agacgcggtg atacatatcc agccatgcac actgatactc 900ttcactccac atgtcggtgt acattgagtg cagcccggct aacgtatcca cgccgtattc 960ggtgatgata atcggctgat gcagtttctc ctgccaggcc agaagttctt tttccagtac 1020cttctctgcc gtttccaaat cgccgctttg gacataccat ccgtaataac ggttcaggca 1080cagcacatca aagagatcgc taatggtatc ggtgtgagcg tcgcagaaca ttacattgac 1140gcaggtgatc ggacgcgtcg ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata 1200ttcccgtgca ccttgcggac gggtatccgg ttcgttggca atactccaca tcaccacgct 1260tgggtggttt ttgtcacgcg ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt 1320ttccccgttg actgcctctt cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc 1380aatccctaaa gagaggttaa agccgacagc agcagtttca tcaatcacca cgatgccatg 1440ttcatctgcc cagtcgagca tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt 1500ggccccaatc cagtccatta atgcgtggtc gtgcaccatc agcacgttat cgaatccttt 1560gccacgcaag tccgcatctt catgacgacc aaagccagta aagtagaacg gtttgtggtt 1620aatcaggaac tgttggccct tcactgccac tgaccggatg ccgacgcgaa gcgggtagat 1680atcacactct gtctggcttt tggctgtgac gcacagttca tagagataac cttcacccgg 1740ttgccagagg tgcggattca ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac 1800cacctgttga tccgcatcac gcagttcaac gctgacatca ccattggcca ccacctgcca 1860gtcaacagac gcgtggttac agtcttgcgc gacatgcgtc accacggtga tatcgtccac 1920ccaggtgttc ggcgtggtgt agagcattac gctgcgatgg attccggcat agttaaagaa 1980atcatggaag taagactgct ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg 2040gatagtctgc cagttcagtt cgttgttcac acaaacggtg atacctgcac atcaacaaat 2100tttggtcata tattagaaaa gttataaatt aaaatataca cacttataaa ctacagaaaa 2160gcaattgcta tatactacat tcttttattt tgaaaaaaat atttgaaata ttatattact 2220actaattaat gataattatt atatatatat caaaggtaga agcagaaact tacgtacact 2280tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg 2340ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg 2400cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac 2460gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac 2520agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt 2580ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt 2640ttctacagga cggaccatgg tgtcgtgtgg atccaaattg tatgcaaggt gaatgacttt 2700cttttcgtaa actagatagg agtactcctc caggatgctt aacccgtatt gacgtacaga 2760ggtctatgat ccttttgttt ataaaggagc ttgtagttca gtcagtctta tacttcacga 2820tgcccatgtt tctatatagg atattatctt ggctttgtaa gtacttcacg caggttatgt 2880tctgtttcta ggatattatc ctcatacatg cgaagaacca atttttcccc cattctcttc 2940gggtactttt tcttgggtag gcatgctctc ttggaccaac tagcataaaa cataatcatt 3000tttccctaca gccttgacca gctataatcg aaatcatgct catttttcta agaaagactg 3060aatacagctc caatttaaac aatttaaatc ataaacttgt aactcaatta gagaaaagca 3120gagcccttcg gctcctatct aaaggaatta ccccatgaaa gccataaaaa cgaaccttgc 3180tctgatacca gacgggtcta cgctcgcgga actaggatct tgcgctctac tcgcacaaag 3240tgaactcgca caaagtgtgt ttcaagcaca gaagttttta tttctcaaat caggagtaaa 3300ctcgcgttgt ggtgcgtgtt tgcaacctga atacaaggct ccttatatag agagttgtgg 3360agctttctgg catcgttagg tggcatccac caataatgca gataagcatc atcacatgtc 3420tctggcctaa caactttgcg taagaatcct gcaaagttac taaaggtcat cgtgcgtgac 3480tagacaacgc acaccgacaa acttaaaata aagagacatt atactttgtc tcctctttac 3540ataaagtgag tggtatccag ctcactccgc atcttatcag tcttcacacc ggttggtatc 3600aacacgtggt aggggtccgc cacttccgct tcagtcatca ttactgatat ccagcagatc 3660tagagcatct tcaataagat attcttgttc tgcacgcaga ttttcttgct ccctcagtaa 3720ttcctcccac agtgagtctt ctgatatttc ttcaagtttc ttctcccatc tgatcttttc 3780ctgcacaaac gagtcaattt ggtctttcca gacccaagta aaacaagtgt tagtttcaca 3840ggagtaaaac tccctgtcag gatttctgga tgttctggag atcttcagtt ttgctggttt 3900attgcatcca catttgaaaa ccggctcttc acttagtgtt agcacattga tttgatgcaa 3960cctgtagcct ttgctcaacc agtcttcata tctttttaca acatcattaa ctctctgttt 4020tgcatcggtg tttcccttgt gaaatacctc ctccactgca ttgatcaaca caccttcaga 4080ttgatgcttt tccggatgga gaataatctt taccagtctt gacagagtgt ctgctaaaac 4140gttgtccttt ccgtcaatgt gttcaaactt aatctcaaga cctgtcccgg taatgtaatc 4200tgtgaaggca agccatctga ctcttgatgg tttatgatca ctgcttttct tgtaaaagct 4260cactattgct tgactgtcag ttctgattat gagctctttg taagcttggt cacccggtcc 4320gggcctagaa ggccagcttc ggccgccccg ggcaacttta ttatacaaag ttgatagata 4380tcggaccgat taaactttaa ttcggtccga agcttgcatg cctgcagtgc agcgtgaccc 4440ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac 4500atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa 4560actttactct acgaataata taatctatag tactacaata atatcagtgt tttagagaat 4620catataaatg aacagttaga catggtctaa aggacaattg agtattttga caacaggact 4680ctacagtttt atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc 4740tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt 4800tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa 4860ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa

4920gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc 4980ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa 5040ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg 5100ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 5160tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 5220ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 5280ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt 5340tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 5400caaggtacgc cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg 5460gtccatgcat ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg 5520tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg 5580ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt 5640ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc 5700ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt 5760ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagtagaat 5820tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat 5880attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg 5940ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga 6000tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac 6060tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac 6120gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta 6180ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc 6240tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga 6300tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt 6360gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggtc 6420gactttaact tagcctagga tccacacgac accatgtccc ccgagcgccg ccccgtcgag 6480atccgcccgg ccaccgccgc cgacatggcc gccgtgtgcg acatcgtgaa ccactacatc 6540gagacctcca ccgtgaactt ccgcaccgag ccgcagaccc cgcaggagtg gatcgacgac 6600ctggagcgcc tccaggaccg ctacccgtgg ctcgtggccg aggtggaggg cgtggtggcc 6660ggcatcgcct acgccggccc gtggaaggcc cgcaacgcct acgactggac cgtggagtcc 6720accgtgtacg tgtcccaccg ccaccagcgc ctcggcctcg gctccaccct ctacacccac 6780ctcctcaaga gcatggaggc ccagggcttc aagtccgtgg tggccgtgat cggcctcccg 6840aacgacccgt ccgtgcgcct ccacgaggcc ctcggctaca ccgcccgcgg caccctccgc 6900gccgccggct acaagcacgg cggctggcac gacgtcggct tctggcagcg cgacttcgag 6960ctgccggccc cgccgcgccc ggtgcgcccg gtgacgcaga tctgagtcga aacctagact 7020tgtccatctt ctggattggc caacttaatt aatgtatgaa ataaaaggat gcacacatag 7080tgacatgcta atcactataa tgtgggcatc aaagttgtgt gttatgtgta attactagtt 7140atctgaataa aagagaaaga gatcatccat atttcttatc ctaaatgaat gtcacgtgtc 7200tttataattc tttgatgaac cagatgcatt tcattaacca aatccatata catataaata 7260ttaatcatat ataattaata tcaattgggt tagcaaaaca aatctagtct aggtgtgttt 7320tgcgaatgcg gccgataagt gactagggtc acgtgaccct agtcacttag gtaccgagct 7380cgaattcatt ccgattaatc gtggcctctt gctcttcagg atgaagagct atgtttaaac 7440gtgcaagcgc tactagacaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg 7500tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc 7560cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg 7620cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg 7680aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt 7740tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga 7800tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga 7860gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc 7920tatttcttta gaagtgaacg ttgacgatcg tcgaccgtac cccgatgaat taattcggac 7980gtacgttctg aacacagctg gatacttact tgggcgattg tcatacatga catcaacaat 8040gtacccgttt gtgtaaccgt ctcttggagg ttcgtatgac actagtggtt cccctcagct 8100tgcgactaga tgttgaggcc taacatttta ttagagagca ggctagttgc ttagatacat 8160gatcttcagg ccgttatctg tcagggcaag cgaaaattgg ccatttatga cgaccaatgc 8220cccgcagaag ctcccatctt tgccgccata gacgccgcgc cccccttttg gggtgtagaa 8280catccttttg ccagatgtgg aaaagaagtt cgttgtccca ttgttggcaa tgacgtagta 8340gccggcgaaa gtgcgagacc catttgcgct atatataagc ctacgatttc cgttgcgact 8400attgtcgtaa ttggatgaac tattatcgta gttgctctca gagttgtcgt aatttgatgg 8460actattgtcg taattgctta tggagttgtc gtagttgctt ggagaaatgt cgtagttgga 8520tggggagtag tcatagggaa gacgagcttc atccactaaa acaattggca ggtcagcaag 8580tgcctgcccc gatgccatcg caagtacgag gcttagaacc accttcaaca gatcgcgcat 8640agtcttcccc agctctctaa cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga 8700acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtgaac 8760aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttgtccaa gataagcctg 8820cctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc 8880agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt 8940aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt 9000ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc 9060tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat 9120gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa 9180ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt 9240gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc 9300gttgatcaaa gctcgccgcg ttgtttcatc aagccttaca gtcaccgtaa ccagcaaatc 9360aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag 9420caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac 9480ttcggcgatc accgcttccc tcatgatgtt taactcctga attaagccgc gccgcgaagc 9540ggtgtcggct tgaatgaatt gttaggcgtc atcctgtgct cccgagaacc agtaccagta 9600catcgctgtt tcgttcgaga cttgaggtct agttttatac gtgaacaggt caatgccgcc 9660gagagtaaag ccacattttg cgtacaaatt gcaggcaggt acattgttcg tttgtgtctc 9720taatcgtatg ccaaggagct gtctgcttag tgcccacttt ttcgcaaatt cgatgagact 9780gtgcgcgact cctttgcctc ggtgcgtgtg cgacacaaca atgtgttcga tagaggctag 9840atcgttccat gttgagttga gttcaatctt cccgacaagc tcttggtcga tgaatgcgcc 9900atagcaagca gagtcttcat cagagtcatc atccgagatg taatccttcc ggtaggggct 9960cacacttctg gtagatagtt caaagccttg gtcggatagg tgcacatcga acacttcacg 10020aacaatgaaa tggttctcag catccaatgt ttccgccacc tgctcaggga tcaccgaaat 10080cttcatatga cgcctaacgc ctggcacagc ggatcgcaaa cctggcgcgg cttttggcac 10140aaaaggcgtg acaggtttgc gaatccgttg ctgccacttg ttaacccttt tgccagattt 10200ggtaactata atttatgtta gaggcgaagt cttgggtaaa aactggccta aaattgctgg 10260ggatttcagg aaagtaaaca tcaccttccg gctcgatgtc tattgtagat atatgtagtg 10320tatctacttg atcgggggat ctgctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc 10380tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 10440caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag 10500tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac 10560tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 10620tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 10680gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 10740caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 10800tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 10860gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 10920ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 10980cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 11040tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 11100tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 11160cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 11220agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 11280agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 11340gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 11400aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 11460ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 11520gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 11580taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 11640tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 11700tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 11760gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 11820gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 11880ttgctgcagg gggggggggg ggggggttcc attgttcatt ccacggacaa aaacagagaa 11940aggaaacgac agaggccaaa aagctcgctt tcagcacctg tcgtttcctt tcttttcaga 12000gggtatttta aataaaaaca ttaagttatg acgaagaaga acggaaacgc cttaaaccgg 12060aaaattttca taaatagcga aaacccgcga ggtcgccgcc ccgtaacctg tcggatcacc 12120ggaaaggacc cgtaaagtga taatgattat catctacata tcacaacgtg cgtggaggcc 12180atcaaaccac gtcaaataat caattatgac gcaggtatcg tattaattga tctgcatcaa 12240cttaacgtaa aaacaacttc agacaataca aatcagcgac actgaatacg gggcaacctc 12300atgtcccccc cccccccccc cctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg 12360cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 12420aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 12480tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 12540gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 12600cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 12660aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 12720tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 12780tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 12840gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 12900atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 12960taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 13020tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattcg 13080gagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac 13140cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca 13200gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 13260cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 13320catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag 13380agcattacgc tgacttgacg ggacggcggc tttgttgaat aaatcgaact tttgctgagt 13440tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt 13500tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt 13560tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag 13620gcagacctca gcgccagaag gccgccagag aggccgagcg cggccgtgag gcttggacgc 13680tagggcaggg catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg cggtggaaag 13740ggggagggga tgttgtctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg 13800tcctgatcaa tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt 13860cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg gaccggcttc 13920gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt caacggtgcc 13980gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc caacagtgaa 14040gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg cctcgcagag 14100gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg 14160gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat 14220cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag 14280catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc 14340cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac 14400ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct 14460tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg 14520cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc caacataccc 14580ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg 14640gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc 14700attctgctgg cgctgtatgc gttggtgcaa tttgcctgcg cacctgtgct gggcgcgctg 14760tcggatcgtt tcgggcggcg gccaatcttg ctcgtctcgc tggccggcgc cactgtcgac 14820tacgccatca tggcgacagc gcctttcctt tgggttctct atatcgggcg gatcgtggcc 14880ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat cactgatggc 14940gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg 15000ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt cttcgccgcg 15060gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa 15120ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc 15180cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca acttgtcgga 15240caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc gctttcactg ggacgcgacc 15300acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca ggcaatgatc 15360accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc 15420gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc gttcccgatc 15480atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat gttgtccagg 15540caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct caccagcctg 15600acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat aacaacgtgg 15660aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc ggcgctgcgt 15720cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa acgataggcc 15780tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga gtgcggttgg 15840aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt tgaagcgcac 15900tcagcgtctg atccaagaac aaccatccta gcaacacggc ggtccccggg ctgagaaagc 15960ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg 16020ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca 16080tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca 16140gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg 16200ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg 16260ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga 16320ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg 16380gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca 16440acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga 16500ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca 16560gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat 16620gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct 16680cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca 16740tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg 16800ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag 16860ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt 16920gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa 16980tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg 17040tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa 17100ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc 17160gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata 17220gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt 17280gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga 17340cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg 17400cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc 17460ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa 17520cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca 17580caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt 17640gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt 17700ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg 17760cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa 17820cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt 17880cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc 17940ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc 18000cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga 18060ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc 18120ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt 18180cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg 18240atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt 18300cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa 18360taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt 18420gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc actcttcatt 18480aaccgctata tcgaaaattg cttgcggctt gttagaattg ccatgacgta cctcggtgtc 18540acgggtaaga ttaccgataa actggaactg attatggctc atatcgaaag tctccttgag 18600aaaggagact ctagtttagc taaacattgg ttccgctgtc aagaacttta gcggctaaaa 18660ttttgcgggc cgcgaccaaa ggtgcgaggg gcggcttccg ctgtgtacaa ccagatattt 18720ttcaccaaca tccttcgtct gctcgatgag cggggcatga cgaaacatga gctgtcggag 18780agggcagggg tttcaatttc gtttttatca gacttaacca acggtaaggc caacccctcg 18840ttgaaggtga tggaggccat tgccgacgcc ctggaaactc ccctacctct tctcctggag 18900tccaccgacc ttgaccgcga ggcactcgcg gagattgcgg gtcatccttt caagagcagc 18960gtgccgcccg gatacgaacg catcagtgtg gttttgccgt cacataaggc gtttatcgta 19020aagaaatggg gcgacgacac ccgaaaaaag ctgcgtggaa ggctctgacg ccaagggtta 19080gggcttgcac ttccttcttt agccgctaaa acggcccctt ctctgcgggc cgtcggctcg 19140cgcatcatat cgacatcctc aacggaagcc gtgccgcgaa tggcatcggg cgggtgcgct 19200ttgacagttg ttttctatca gaacccctac gtcgtgcggt tcgattagct gtttgtcttg 19260caggctaaac actttcggta tatcgtttgc ctgtgcgata atgttgctaa tgatttgttg 19320cgtaggggtt actgaaaagt gagcgggaaa gaagagtttc agaccatcaa ggagcgggcc 19380aagcgcaagc tggaacgcga catgggtgcg gacctgttgg ccgcgctcaa cgacccgaaa 19440accgttgaag tcatgctcaa cgcggacggc aaggtgtggc acgaacgcct tggcgagccg 19500atgcggtaca tctgcgacat gcggcccagc cagtcgcagg cgattataga aacggtggcc 19560ggattccacg gcaaagaggt cacgcggcat tcgcccatcc tggaaggcga gttccccttg 19620gatggcagcc gctttgccgg ccaattgccg ccggtcgtgg ccgcgccaac ctttgcgatc 19680cgcaagcgcg cggtcgccat cttcacgctg gaacagtacg tcgaggcggg catcatgacc 19740cgcgagcaat acgaggtcat taaaagcgcc gtcgcggcgc atcgaaacat cctcgtcatt 19800ggcggtactg gctcgggcaa gaccacgctc gtcaacgcga tcatcaatga aatggtcgcc 19860ttcaacccgt ctgagcgcgt cgtcatcatc gaggacaccg gcgaaatcca gtgcgccgca 19920gagaacgccg tccaatacca caccagcatc gacgtctcga tgacgctgct gctcaagaca

19980acgctgcgta tgcgccccga ccgcatcctg gtcggtgagg tacgtggccc cgaagccctt 20040gatctgttga tggcctggaa caccgggcat gaaggaggtg ccgccaccct gcacgcaaac 20100aaccccaaag cgggcctgag ccggctcgcc atgcttatca gcatgcaccc ggattcaccg 20160aaacccattg agccgctgat tggcgaggcg gttcatgtgg tcgtccatat cgccaggacc 20220cctagcggcc gtcgagtgca agaaattctc gaagttcttg gttacgagaa cggccagtac 20280atcaccaaaa ccctgtaagg agtatttcca atgacaacgg ctgttccgtt ccgtctgacc 20340atgaatcgcg gcattttgtt ctaccttgcc gtgttcttcg ttctcgctct cgcgttatcc 20400gcgcatccgg cgatggcctc ggaaggcacc ggcggcagct tgccatatga gagctggctg 20460acgaacctgc gcaactccgt aaccggcccg gtggccttcg cgctgtccat catcggcatc 20520gtcgtcgccg gcggcgtgct gatcttcggc ggcgaactca acgccttctt ccgaaccctg 20580atcttcctgg ttctggtgat ggcgctgctg gtcggcgcgc agaacgtgat gagcaccttc 20640ttcggtcgtg gtgccgaaat cgcggccctc ggcaacgggg cgctgcacca ggtgcaagtc 20700gcggcggcgg atgccgtgcg tgcggtagcg gctggacggc tcgcctaatc atggctctgc 20760gcacgatccc catccgtcgc gcaggcaacc gagaaaacct gttcatgggt ggtgatcgtg 20820aactggtgat gttctcgggc ctgatggcgt ttgcgctgat tttcagcgcc caagagctgc 20880gggccaccgt ggtcggtctg atcctgtggt tcggggcgct ctatgcgttc cgaatcatgg 20940cgaaggccga tccgaagatg cggttcgtgt acctgcgtca ccgccggtac aagccgtatt 21000acccggcccg ctcgaccccg ttccgcgaga acaccaatag ccaagggaag caataccgat 21060gatccaagca attgcgattg caatcgcggg cctcggcgcg cttctgttgt tcatcctctt 21120tgcccgcatc cgcgcggtcg atgccgaact gaaactgaaa aagcatcgtt ccaaggacgc 21180cggcctggcc gatctgctca actacgccgc tgtcgtcgat gacggcgtaa tcgtgggcaa 21240gaacggcagc tttatggctg cctggctgta caagggcgat gacaacgcaa gcagcaccga 21300ccagcagcgc gaagtagtgt ccgcccgcat caaccaggcc ctcgcgggcc tgggaagtgg 21360gtggatgatc catgtggacg ccgtgcggcg tcctgctccg aactacgcgg agcggggcct 21420gtcggcgttc cctgaccgtc tgacggcagc gattgaagaa gagcgctcgg tcttgccttg 21480ctcgtcggtg atgtacttca ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg 21540gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct 21600ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga 21660tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg 21720tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct 21780cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca 21840ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt 21900ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag 21960ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat 22020tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg 22080gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta 22140cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa 22200tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc cctgctgttt 22260tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac tgaggggaca 22320ggcgagagac gatgccaaag agctacaccg acgagctggc cgagtgggtt gaatcccgcg 22380cggccaagaa gcgccggcgt gatgaggctg cggttgcgtt cctggcggtg agggcggatg 22440tcgaggcggc gttagcgtcc ggctatgcgc tcgtcaccat ttgggagcac atgcgggaaa 22500cggggaaggt caagttctcc tacgagacgt tccgctcgca cgccaggcgg cacatcaagg 22560ccaagcccgc cgatgtgccc gcaccgcagg ccaaggctgc ggaacccgcg ccggcaccca 22620agacgccgga gccacggcgg ccgaagcagg ggggcaaggc tgaaaagccg gcccccgctg 22680cggccccgac cggcttcacc ttcaacccaa caccggacaa aaaggatcta ctgtaatggc 22740gaaaattcac atggttttgc agggcaaggg cggggtcggc aagtcggcca tcgccgcgat 22800cattgcgcag tacaagatgg acaaggggca gacacccttg tgcatcgaca ccgacccggt 22860gaacgcgacg ttcgagggct acaaggccct gaacgtccgc cggctgaaca tcatggccgg 22920cgacgaaatt aactcgcgca acttcgacac cctggtcgag ctgattgcgc cgaccaagga 22980tgacgtggtg atcgacaacg gtgccagctc gttcgtgcct ctgtcgcatt acctcatcag 23040caaccaggtg ccggctctgc tgcaagaaat ggggcatgag ctggtcatcc ataccgtcgt 23100caccggcggc caggctctcc tggacacggt gagcggcttc gcccagctcg ccagccagtt 23160cccggccgaa gcgcttttcg tggtctggct gaacccgtat tgggggccta tcgagcatga 23220gggcaagagc tttgagcaga tgaaggcgta cacggccaac aaggcccgcg tgtcgtccat 23280catccagatt ccggccctca aggaagaaac ctacggccgc gatttcagcg acatgctgca 23340agagcggctg acgttcgacc aggcgctggc cgatgaatcg ctcacgatca tgacgcggca 23400acgcctcaag atcgtgcggc gcggcctgtt tgaacagctc gacgcggcgg ccgtgctatg 23460agcgaccaga ttgaagagct gatccgggag attgcggcca agcacggcat cgccgtcggc 23520cgcgacgacc cggtgctgat cctgcatacc atcaacgccc ggctcatggc cgacagtgcg 23580gccaagcaag aggaaatcct tgccgcgttc aaggaagagc tggaagggat cgcccatcgt 23640tggggcgagg acgccaaggc caaagcggag cggatgctga acgcggccct ggcggccagc 23700aaggacgcaa tggcgaaggt aatgaaggac agcgccgcgc aggcggccga agcgatccgc 23760agggaaatcg acgacggcct tggccgccag ctcgcggcca aggtcgcgga cgcgcggcgc 23820gtggcgatga tgaacatgat cgccggcggc atggtgttgt tcgcggccgc cctggtggtg 23880tgggcctcgt tatgaatcgc agaggcgcag atgaaaaagc ccggcgttgc cgggctttgt 23940ttttgcgtta gctgggcttg tttgacaggc ccaagctctg actgcgcccg cgctcgcgct 24000cctgggcctg tttcttctcc tgctcctgct tgcgcatcag ggcctggtgc cgtcgggctg 24060cttcacgcat cgaatcccag tcgccggcca gctcgggatg ctccgcgcgc atcttgcgcg 24120tcgccagttc ctcgatcttg ggcgcgtgaa tgcccatgcc ttccttgatt tcgcgcacca 24180tgtccagccg cgtgtgcagg gtctgcaagc gggcttgctg ttgggcctgc tgctgctgcc 24240aggcggcctt tgtacgcggc agggacagca agccgggggc attggactgt agctgctgca 24300aacgcgcctg ctgacggtct acgagctgtt ctaggcggtc ctcgatgcgc tccacctggt 24360catgctttgc ctgcacgtag agcgcaaggg tctgctggta ggtctgctcg atgggcgcgg 24420attctaagag ggcctgctgt tccgtctcgg cctcctgggc cgcctgtagc aaatcctcgc 24480cgctgttgcc gctggactgc tttactgccg gggactgctg ttgccctgct cgcgccgtcg 24540tcgcagttcg gcttgccccc actcgattga ctgcttcatt tcgagccgca gcgatgcgat 24600ctcggattgc gtcaacggac ggggcagcgc ggaggtgtcc ggcttctcct tgggtgagtc 24660ggtcgatgcc atagccaaag gtttccttcc aaaatgcgtc cattgctgga ccgtgtttct 24720cattgatgcc cgcaagcatc ttcggcttga ccgccaggtc aagcgcgcct tcatgggcgg 24780tcatgacgga cgccgccatg accttgccgc cgttgttctc gatgtagccg cgtaatgagg 24840caatggtgcc gcccatcgtc agcgtgtcat cgacaacgat gtacttctgg ccggggatca 24900cctccccctc gaaagtcggg ttgaacgcca ggcgatgatc tgaaccggct ccggttcggg 24960cgaccttctc ccgctgcaca atgtccgttt cgacctcaag gccaaggcgg tcggccagaa 25020cgaccgccat catggccgga atcttgttgt tccccgccgc ctcgacggcg aggactggaa 25080cgatgcgggg cttgtcgtcg ccgatcagcg tcttgagctg ggcaacagtg tcgtccgaaa 25140tcaggcgctc gaccaaatta agcgccgctt ccgcgtcgcc ctgcttcgca gcctggtatt 25200caggctcgtt ggtcaaagaa ccaaggtcgc cgttgcgaac caccttcggg aagtctcccc 25260acggtgcgcg ctcggctctg ctgtagctgc tcaagacgcc tcccttttta gccgctaaaa 25320ctctaacgag tgcgcccgcg actcaacttg acgctttcgg cacttacctg tgccttgcca 25380cttgcgtcat aggtgatgct tttcgcactc ccgatttcag gtactttatc gaaatctgac 25440cgggcgtgca ttacaaagtt cttccccacc tgttggtaaa tgctgccgct atctgcgtgg 25500acgatgctgc cgtcgtggcg ctgcgactta tcggcctttt gggccatata gatgttgtaa 25560atgccaggtt tcagggcccc ggctttatct accttctggt tcgtccatgc gccttggttc 25620tcggtctgga caattctttg cccattcatg accaggaggc ggtgtttcat tgggtgactc 25680ctgacggttg cctctggtgt taaacgtgtc ctggtcgctt gccggctaaa aaaaagccga 25740cctcggcagt tcgaggccgg ctttccctag agccgggcgc gtcaaggttg ttccatctat 25800tttagtgaac tgcgttcgat ttatcagtta ctttcctccc gctttgtgtt tcctcccact 25860cgtttccgcg tctagccgac ccctcaacat agcggcctct tcttgggctg cctttgcctc 25920ttgccgcgct tcgtcacgct cggcttgcac cgtcgtaaag cgctcggcct gcctggccgc 25980ctcttgcgcc gccaacttcc tttgctcctg gtgggcctcg gcgtcggcct gcgccttcgc 26040tttcaccgct gccaactccg tgcgcaaact ctccgcttcg cgcctggtgg cgtcgcgctc 26100gccgcgaagc gcctgcattt cctggttggc cgcgtccagg gtcttgcggc tctcttcttt 26160gaatgcgcgg gcgtcctggt gagcgtagtc cagctcggcg cgcagctcct gcgctcgacg 26220ctccacctcg tcggcccgct gcgtcgccag cgcggcccgc tgctcggctc ctgccagggc 26280ggtgcgtgct tcggccaggg cttgccgctg gcgtgcggcc agctcggccg cctcggcggc 26340ctgctgctct agcaatgtaa cgcgcgcctg ggcttcttcc agctcgcggg cctgcgcctc 26400gaaggcgtcg gccagctccc cgcgcacggc ttccaactcg ttgcgctcac gatcccagcc 26460ggcttgcgct gcctgcaacg attcattggc aagggcctgg gcggcttgcc agagggcggc 26520cacggcctgg ttgccggcct gctgcaccgc gtccggcacc tggactgcca gcggggcggc 26580ctgcgccgtg cgctggcgtc gccattcgcg catgccggcg ctggcgtcgt tcatgttgac 26640gcgggcggcc ttacgcactg catccacggt cgggaagttc tcccggtcgc cttgctcgaa 26700cagctcgtcc gcagccgcaa aaatgcggtc gcgcgtctct ttgttcagtt ccatgttggc 26760tccggtaatt ggtaagaata ataatactct tacctacctt atcagcgcaa gagtttagct 26820gaacagttct cgacttaacg gcaggttttt tagcggctga agggcaggca aaaaaagccc 26880cgcacggtcg gcgggggcaa agggtcagcg ggaaggggat tagcgggcgt cgggcttctt 26940catgcgtcgg ggccgcgctt cttgggatgg agcacgacga agcgcgcacg cgcatcgtcc 27000tcggccctat cggcccgcgt cgcggtcagg aacttgtcgc gcgctaggtc ctccctggtg 27060ggcaccaggg gcatgaactc ggcctgctcg atgtaggtcc actccatgac cgcatcgcag 27120tcgaggccgc gttccttcac cgtctcttgc aggtcgcggt acgcccgctc gttgagcggc 27180tggtaacggg ccaattggtc gtaaatggct gtcggccatg agcggccttt cctgttgagc 27240cagcagccga cgacgaagcc ggcaatgcag gcccctggca caaccaggcc gacgccgggg 27300gcaggggatg gcagcagctc gccaaccagg aaccccgccg cgatgatgcc gatgccggtc 27360aaccagccct tgaaactatc cggccccgaa acacccctgc gcattgcctg gatgctgcgc 27420cggatagctt gcaacatcag gagccgtttc ttttgttcgt cagtcatggt ccgccctcac 27480cagttgttcg tatcggtgtc ggacgaactg aaatcgcaag agctgccggt atcggtccag 27540ccgctgtccg tgtcgctgct gccgaagcac ggcgaggggt ccgcgaacgc cgcagacggc 27600gtatccggcc gcagcgcatc gcccagcatg gccccggtca gcgagccgcc ggccaggtag 27660cccagcatgg tgctgttggt cgccccggcc accagggccg acgtgacgaa atcgccgtca 27720ttccctctgg attgttcgct gctcggcggg gcagtgcgcc gcgccggcgg cgtcgtggat 27780ggctcgggtt ggctggcctg cgacggccgg cgaaaggtgc gcagcagctc gttatcgacc 27840ggctgcggcg tcggggccgc cgccttgcgc tgcggtcggt gttccttctt cggctcgcgc 27900agcttgaaca gcatgatcgc ggaaaccagc agcaacgccg cgcctacgcc tcccgcgatg 27960tagaacagca tcggattcat tcttcggtcc tccttgtagc ggaaccgttg tctgtgcggc 28020gcgggtggcc cgcgccgctg tctttgggga tcagccctcg atgagcgcga ccagtttcac 28080gtcggcaagg ttcgcctcga actcctggcc gtcgtcctcg tacttcaacc aggcatagcc 28140ttccgccggc ggccgacggt tgaggataag gcgggcaggg cgctcgtcgt gctcgacctg 28200gacgatggcc tttttcagct tgtccgggtc cggctccttc gcgccctttt ccttggcgtc 28260cttaccgtcc tggtcgccgt cctcgccgtc ctggccgtcg ccggcctccg cgtcacgctc 28320ggcatcagtc tggccgttga aggcatcgac ggtgttggga tcgcggccct tctcgtccag 28380gaactcgcgc agcagcttga ccgtgccgcg cgtgatttcc tgggtgtcgt cgtcaagcca 28440cgcctcgact tcctccgggc gcttcttgaa ggccgtcacc agctcgttca ccacggtcac 28500gtcgcgcacg cggccggtgt tgaacgcatc ggcgatcttc tccggcaggt ccagcagcgt 28560gacgtgctgg gtgatgaacg ccggcgactt gccgatttcc ttggcgatat cgcctttctt 28620cttgcccttc gccagctcgc ggccaatgaa gtcggcaatt tcgcgcgggg tcagctcgtt 28680gcgttgcagg ttctcgataa cctggtcggc ttcgttgtag tcgttgtcga tgaacgccgg 28740gatggacttc ttgccggccc acttcgagcc acggtagcgg cgggcgccgt gattgatgat 28800atagcggccc ggctgctcct ggttctcgcg caccgaaatg ggtgacttca ccccgcgctc 28860tttgatcgtg gcaccgattt ccgcgatgct ctccggggaa aagccggggt tgtcggccgt 28920ccgcggctga tgcggatctt cgtcgatcag gtccaggtcc agctcgatag ggccggaacc 28980gccctgagac gccgcaggag cgtccaggag gctcgacagg tcgccgatgc tatccaaccc 29040caggccggac ggctgcgccg cgcctgcggc ttcctgagcg gccgcagcgg tgtttttctt 29100ggtggtcttg gcttgagccg cagtcattgg gaaatctcca tcttcgtgaa cacgtaatca 29160gccagggcgc gaacctcttt cgatgccttg cgcgcggccg ttttcttgat cttccagacc 29220ggcacaccgg atgcgagggc atcggcgatg ctgctgcgca ggccaacggt ggccggaatc 29280atcatcttgg ggtacgcggc cagcagctcg gcttggtggc gcgcgtggcg cggattccgc 29340gcatcgacct tgctgggcac catgccaagg aattgcagct tggcgttctt ctggcgcacg 29400ttcgcaatgg tcgtgaccat cttcttgatg ccctggatgc tgtacgcctc aagctcgatg 29460ggggacagca catagtcggc cgcgaagagg gcggccgcca ggccgacgcc aagggtcggg 29520gccgtgtcga tcaggcacac gtcgaagcct tggttcgcca gggccttgat gttcgccccg 29580aacagctcgc gggcgtcgtc cagcgacagc cgttcggcgt tcgccagtac cgggttggac 29640tcgatgaggg cgaggcgcgc ggcctggccg tcgccggctg cgggtgcggt ttcggtccag 29700ccgccggcag ggacagcgcc gaacagcttg cttgcatgca ggccggtagc aaagtccttg 29760agcgtgtagg acgcattgcc ctgggggtcc aggtcgatca cggcaacccg caagccgcgc 29820tcgaaaaagt cgaaggcaag atgcacaagg gtcgaagtct tgccgacgcc gcctttctgg 29880ttggccgtga ccaaagtttt catcgtttgg tttcctgttt tttcttggcg tccgcttccc 29940acttccggac gatgtacgcc tgatgttccg gcagaaccgc cgttacccgc gcgtacccct 30000cgggcaagtt cttgtcctcg aacgcggccc acacgcgatg caccgcttgc gacactgcgc 30060ccctggtcag tcccagcgac gttgcgaacg tcgcctgtgg cttcccatcg actaagacgc 30120cccgcgctat ctcgatggtc tgctgcccca cttccagccc ctggatcgcc tcctggaact 30180ggctttcggt aagccgtttc ttcatggata acacccataa tttgctccgc gccttggttg 30240aacatagcgg tgacagccgc cagcacatga gagaagttta gctaaacatt tctcgcacgt 30300caacaccttt agccgctaaa actcgtcctt ggcgtaacaa aacaaaagcc cggaaaccgg 30360gctttcgtct cttgccgctt atggctctgc acccggctcc atcaccaaca ggtcgcgcac 30420gcgcttcact cggttgcgga tcgacactgc cagcccaaca aagccggttg ccgccgccgc 30480caggatcgcg ccgatgatgc cggccacacc ggccatcgcc caccaggtcg ccgccttccg 30540gttccattcc tgctggtact gcttcgcaat gctggacctc ggctcaccat aggctgaccg 30600ctcgatggcg tatgccgctt ctccccttgg cgtaaaaccc agcgccgcag gcggcattgc 30660catgctgccc gccgctttcc cgaccacgac gcgcgcacca ggcttgcggt ccagaccttc 30720ggccacggcg agctgcgcaa ggacataatc agccgccgac ttggctccac gcgcctcgat 30780cagctcttgc actcgcgcga aatccttggc ctccacggcc gccatgaatc gcgcacgcgg 30840cgaaggctcc gcagggccgg cgtcgtgatc gccgccgaga atgcccttca ccaagttcga 30900cgacacgaaa atcatgctga cggctatcac catcatgcag acggatcgca cgaacccgct 30960gaattgaaca cgagcacggc acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt 31020gccggccccg ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca 31080cccaggccgc cgccctcact gcccggcacc tggtcgctga atgtcgatgc cagcacctgc 31140ggcacgtcaa tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt tactgccccg 31200atcccggcaa tggcaaggac tgccagcgct gccatttttg gggtgaggcc gttcgcggcc 31260gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga 31320agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa 31380aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa 31440aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt 31500caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc 31560tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct 31620tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc 31680tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc 31740gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt 31800ttccgcgagg tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt 31860ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc 31920ggtgagcgtc ggaaaggcgc tggaagcccc gtagcgacgc ggagaggggc gagacaagcc 31980aagggcgcag gctcgatgcg cagcacgaca tagccggttc tcgcaaggac gagaatttcc 32040ctgcggtgcc cctcaagtgt caatgaaagt ttccaacgcg agccattcgc gagagccttg 32100agtccacgct agatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc 32160tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca 32220aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca 32280caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca 32340aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgactctaga gctcgttcct 32400cgaggcctcg aggcctcgag gaacggtacc tgcggggaag cttacaataa tgtgtgttgt 32460taagtcttgt tgcctgtcat cgtctgactg actttcgtca taaatcccgg cctccgtaac 32520ccagctttgg gcaagctcac ggatttgatc cggcggaacg ggaatatcga gatgccgggc 32580tgaacgctgc agttccagct ttccctttcg ggacaggtac tccagctgat tgattatctg 32640ctgaagggtc ttggttccac ctcctggcac aatgcgaatg attacttgag cgcgatcggg 32700catccaattt tctcccgtca ggtgcgtggt caagtgctac aaggcacctt tcagtaacga 32760gcgaccgtcg atccgtcgcc gggatacgga caaaatggag cgcagtagtc catcgagggc 32820ggcgaaagcc tcgccaaaag caatacgttc atctcgcaca gcctccagat ccgatcgagg 32880gtcttcggcg taggcagata gaagcatgga tacattgctt gagagtattc cgatggactg 32940aagtatggct tccatctttt ctcgtgtgtc tgcatctatt tcgagaaagc ccccgatgcg 33000gcgcaccgca acgcgaattg ccatactatc cgaaagtccc agcaggcgcg cttgatagga 33060aaaggtttca tactcggccg atcgcagacg ggcactcacg accttgaacc cttcaacttt 33120cagggatcga tgctggttga tggtagtctc actcgacgtg gctctggtgt gttttgacat 33180agcttcctcc aaagaaagcg gaaggtctgg atactccagc acgaaatgtg cccgggtaga 33240cggatggaag tctagccctg ctcaatatga aatcaacagt acatttacag tcaatactga 33300atatacttgc tacatttgca attgtcttat aacgaatgtg aaataaaaat agtgtaacaa 33360cgcttttact catcgataat cacaaaaaca tttatacgaa caaaaataca aatgcactcc 33420ggtttcacag gataggcggg atcagaatat gcaacttttg acgttttgtt ctttcaaagg 33480gggtgctggc aaaaccaccg cactcatggg cctttgcgct gctttggcaa atgacggtaa 33540acgagtggcc ctctttgatg ccgacgaaaa ccggcctctg acgcgatgga gagaaaacgc 33600cttacaaagc agtactggga tcctcgctgt gaagtctatt ccgccgacga aatgcccctt 33660cttgaagcag cctatgaaaa tgccgagctc gaaggatttg attatgcgtt ggccgatacg 33720cgtggcggct cgagcgagct caacaacaca atcatcgcta gctcaaacct gcttctgatc 33780cccaccatgc taacgccgct cgacatcgat gaggcactat ctacctaccg ctacgtcatc 33840gagctgctgt tgagtgaaaa tttggcaatt cctacagctg ttttgcgcca acgcgtcccg 33900gtcggccgat tgacaacatc gcaacgcagg atgtcagaga cgctagagag ccttccagtt 33960gtaccgtctc ccatgcatga aagagatgca tttgccgcga tgaaagaacg cggcatgttg 34020catcttacat tactaaacac gggaactgat ccgacgatgc gcctcataga gaggaatctt 34080cggattgcga tggaggaagt cgtggtcatt tcgaaactga tcagcaaaat cttggaggct 34140tgaagatggc aattcgcaag cccgcattgt cggtcggcga agcacggcgg cttgctggtg 34200ctcgacccga gatccaccat cccaacccga cacttgttcc ccagaagctg gacctccagc 34260acttgcctga aaaagccgac gagaaagacc agcaacgtga gcctctcgtc gccgatcaca 34320tttacagtcc cgatcgacaa cttaagctaa ctgtggatgc ccttagtcca cctccgtccc 34380cgaaaaagct ccaggttttt ctttcagcgc gaccgcccgc gcctcaagtg tcgaaaacat 34440atgacaacct cgttcggcaa tacagtccct cgaagtcgct acaaatgatt ttaaggcgcg 34500cgttggacga tttcgaaagc atgctggcag atggatcatt tcgcgtggcc ccgaaaagtt 34560atccgatccc ttcaactaca gaaaaatccg ttctcgttca gacctcacgc atgttcccgg 34620ttgcgttgct cgaggtcgct cgaagtcatt ttgatccgtt ggggttggag accgctcgag 34680ctttcggcca caagctggct accgccgcgc tcgcgtcatt ctttgctgga gagaagccat 34740cgagcaattg gtgaagaggg acctatcgga acccctcacc aaatattgag tgtaggtttg 34800aggccgctgg ccgcgtcctc agtcaccttt tgagccagat aattaagagc caaatgcaat 34860tggctcaggc tgccatcgtc cccccgtgcg aaacctgcac gtccgcgtca aagaaataac 34920cggcacctct tgctgttttt atcagttgag ggcttgacgg atccgcctca agtttgcggc 34980gcagccgcaa aatgagaaca tctatactcc tgtcgtaaac ctcctcgtcg cgtactcgac

35040tggcaatgag aagttgctcg cgcgatagaa cgtcgcgggg tttctctaaa aacgcgagga 35100gaagattgaa ctcacctgcc gtaagtttca cctcaccgcc agcttcggac atcaagcgac 35160gttgcctgag attaagtgtc cagtcagtaa aacaaaaaga ccgtcggtct ttggagcgga 35220caacgttggg gcgcacgcgc aaggcaaccc gaatgcgtgc aagaaactct ctcgtactaa 35280acggcttagc gataaaatca cttgctccta gctcgagtgc aacaacttta tccgtctcct 35340caaggcggtc gccactgata attatgattg gaatatcaga ctttgccgcc agatttcgaa 35400cgatctcaag cccatcttca cgacctaaat ttagatcaac aaccacgaca tcgaccgtcg 35460cggaagagag tactctagtg aactgggtgc tgtcggctac cgcggtcact ttgaaggcgt 35520ggatcgtaag gtattcgata ataagatgcc gcatagcgac atcgtcatcg ataagaagaa 35580cgtgtttcaa cggctcacct ttcaatctaa aatctgaacc cttgttcaca gcgcttgaga 35640aattttcacg tgaaggatgt acaatcatct ccagctaaat gggcagttcg tcagaattgc 35700ggctgaccgc ggatgacgaa aatgcgaacc aagtatttca attttatgac aaaagttctc 35760aatcgttgtt acaagtgaaa cgcttcgagg ttacagctac tattgattaa ggagatcgcc 35820tatggtctcg ccccggcgtc gtgcgtccgc cgcgagccag atctcgccta cttcataaac 35880gtcctcatag gcacggaatg gaatgatgac atcgatcgcc gtagagagca tgtcaatcag 35940tgtgcgatct tccaagctag caccttgggc gctacttttg acaagggaaa acagtttctt 36000gaatccttgg attggattcg cgccgtgtat tgttgaaatc gatcccggat gtcccgagac 36060gacttcactc agataagccc atgctgcatc gtcgcgcatc tcgccaagca atatccggtc 36120cggccgcata cgcagacttg cttggagcaa gtgctcggcg ctcacagcac ccagcccagc 36180accgttcttg gagtagagta gtctaacatg attatcgtgt ggaatgacga gttcgagcgt 36240atcttctatg gtgattagcc tttcctgggg ggggatggcg ctgatcaagg tcttgctcat 36300tgttgtcttg ccgcttccgg tagggccaca tagcaacatc gtcagtcggc tgacgacgca 36360tgcgtgcaga aacgcttcca aatccccgtt gtcaaaatgc tgaaggatag cttcatcatc 36420ctgattttgg cgtttccttc gtgtctgcca ctggttccac ctcgaagcat cataacggga 36480ggagacttct ttaagaccag aaacacgcga gcttggccgt cgaatggtca agctgacggt 36540gcccgaggga acggtcggcg gcagacagat ttgtagtcgt tcaccaccag gaagttcagt 36600ggcgcagagg gggttacgtg gtccgacatc ctgctttctc agcgcgcccg ctaaaatagc 36660gatatcttca agatcatcat aagagacggg caaaggcatc ttggtaaaaa tgccggcttg 36720gcgcacaaat gcctctccag gtcgattgat cgcaatttct tcagtcttcg ggtcatcgag 36780ccattccaaa atcggcttca gaagaaagcg tagttgcgga tccacttcca tttacaatgt 36840atcctatctc taagcggaaa tttgaattca ttaagagcgg cggttcctcc cccgcgtggc 36900gccgccagtc aggcggagct ggtaaacacc aaagaaatcg aggtcccgtg ctacgaaaat 36960ggaaacggtg tcaccctgat tcttcttcag ggttggcggt atgttgatgg ttgccttaag 37020ggctgtctca gttgtctgct caccgttatt ttgaaagctg ttgaagctca tcccgccacc 37080cgagctgccg gcgtaggtgc tagctgcctg gaaggcgcct tgaacaacac tcaagagcat 37140agctccgcta aaacgctgcc agaagtggct gtcgaccgag cccggcaatc ctgagcgacc 37200gagttcgtcc gcgcttggcg atgttaacga gatcatcgca tggtcaggtg tctcggcgcg 37260atcccacaac acaaaaacgc gcccatctcc ctgttgcaag ccacgctgta tttcgccaac 37320aacggtggtg ccacgatcaa gaagcacgat attgttcgtt gttccacgaa tatcctgagg 37380caagacacac tttacatagc ctgccaaatt tgtgtcgatt gcggtttgca agatgcacgg 37440aattattgtc ccttgcgtta ccataaaatc ggggtgcggc aagagcgtgg cgctgctggg 37500ctgcagctcg gtgggtttca tacgtatcga caaatcgttc tcgccggaca cttcgccatt 37560cggcaaggag ttgtcgtcac gcttgccttc ttgtcttcgg cccgtgtcgc cctgaatggc 37620gcgtttgctg accccttgat cgccgctgct atatgcaaaa atcggtgttt cttccggccg 37680tggctcatgc cgctccggtt cgcccctcgg cggtagagga gcagcaggct gaacagcctc 37740ttgaaccgct ggaggatccg gcggcacctc aatcggagct ggatgaaatg gcttggtgtt 37800tgttgcgatc aaagttgacg gcgatgcgtt ctcattcacc ttcttttggc gcccacctag 37860ccaaatgagg cttaatgata acgcgagaac gacacctccg acgatcaatt tctgagaccc 37920cgaaagacgc cggcgatgtt tgtcggagac cagggatcca gatgcatcaa cctcatgtgc 37980cgcttgctga ctatcgttat tcatcccttc gcccccttca ggacgcgttt cacatcgggc 38040ctcaccgtgc ccgtttgcgg cctttggcca acgggatcgt aagcggtgtt ccagatacat 38100agtactgtgt ggccatccct cagacgccaa cctcgggaaa ccgaagaaat ctcgacatcg 38160ctccctttaa ctgaatagtt ggcaacagct tccttgccat caggattgat ggtgtagatg 38220gagggtatgc gtacattgcc cggaaagtgg aataccgtcg taaatccatt gtcgaagact 38280tcgagtggca acagcgaacg atcgccttgg gcgacgtagt gccaattact gtccgccgca 38340ccaagggctg tgacaggctg atccaataaa ttctcagctt tccgttgata ttgtgcttcc 38400gcgtgtagtc tgtccacaac agccttctgt tgtgcctccc ttcgccgagc cgccgcatcg 38460tcggcggggt aggcgaattg gacgctgtaa tagagatcgg gctgctcttt atcgaggtgg 38520gacagagtct tggaacttat actgaaaaca taacggcgca tcccggagtc gcttgcggtt 38580agcacgatta ctggctgagg cgtgaggacc tggcttgcct tgaaaaatag ataatttccc 38640cgcggtaggg ctgctagatc tttgctattt gaaacggcaa ccgctgtcac cgtttcgttc 38700gtggcgaatg ttacgaccaa agtagctcca accgccgtcg agaggcgcac cacttgatcg 38760ggattgtaag ccaaataacg catgcgcgga tctagcttgc ccgccattgg agtgtcttca 38820gcctccgcac cagtcgcagc ggcaaataaa catgctaaaa tgaaaagtgc ttttctgatc 38880atggttcgct gtggcctacg tttgaaacgg tatcttccga tgtctgatag gaggtgacaa 38940ccagacctgc cgggttggtt agtctcaatc tgccgggcaa gctggtcacc ttttcgtagc 39000gaactgtcgc ggtccacgta ctcaccacag gcattttgcc gtcaacgacg agggtccttt 39060tatagcgaat ttgctgcgtg cttggagtta catcatttga agcgatgtgc tcgacctcca 39120ccctgccgcg tttgccaaga atgacttgag gcgaactggg attgggatag ttgaagaatt 39180gctggtaatc ctggcgcact gttggggcac tgaagttcga taccaggtcg taggcgtact 39240gagcggtgtc ggcatcataa ctctcgcgca ggcgaacgta ctcccacaat gaggcgttaa 39300cgacggcctc ctcttgagtt gcaggcaatc gcgagacaga cacctcgctg tcaacggtgc 39360cgtccggccg tatccataga tatacgggca caagcctgct caacggcacc attgtggcta 39420tagcgaacgc ttgagcaaca tttcccaaaa tcgcgatagc tgcgacagct gcaatgagtt 39480tggagagacg tcgcgccgat ttcgctcgcg cggtttgaaa ggcttctact tccttatagt 39540gctcggcaag gctttcgcgc gccactagca tggcatattc aggccccgtc atagcgtcca 39600cccgaattgc cgagctgaag atctgacgga gtaggctgcc atcgccccac attcagcggg 39660aagatcgggc ctttgcagct cgctaatgtg tcgtttgtct ggcagccgct caaagcgaca 39720actaggcaca gcaggcaata cttcatagaa ttctccattg aggcgaattt ttgcgcgacc 39780tagcctcgct caacctgagc gaagcgacgg tacaagctgc tggcagattg ggttgcgccg 39840ctccagtaac tgcctccaat gttgccggcg atcgccggca aagcgacaat gagcgcatcc 39900cctgtcagaa aaaacatatc gagttcgtaa agaccaatga tcttggccgc ggtcgtaccg 39960gcgaaggtga ttacaccaag cataagggtg agcgcagtcg cttcggttag gatgacgatc 40020gttgccacga ggtttaagag gagaagcaag agaccgtagg tgataagttg cccgatccac 40080ttagctgcga tgtcccgcgt gcgatcaaaa atatatccga cgaggatcag aggcccgatc 40140gcgagaagca ctttcgtgag aattccaacg gcgtcgtaaa ctccgaaggc agaccagagc 40200gtgccgtaaa ggacccactg tgccccttgg aaagcaagga tgtcctggtc gttcatcgga 40260ccgatttcgg atgcgatttt ctgaaaaacg gcctgggtca cggcgaacat tgtatccaac 40320tgtgccggaa cagtctgcag aggcaagccg gttacactaa actgctgaac aaagtttggg 40380accgtctttt cgaagatgga aaccacatag tcttggtagt tagcctgccc aacaattaga 40440gcaacaacga tggtgaccgt gatcacccga gtgataccgc tacgggtatc gacttcgccg 40500cgtatgacta aaataccctg aacaataatc caaagagtga cacaggcgat caatggcgca 40560ctcaccgcct cctggatagt ctcaagcatc gagtccaagc ctgtcgtgaa ggctacatcg 40620aagatcgtat gaatggccgt aaacggcgcc ggaatcgtga aattcatcga ttggacctga 40680acttgactgg tttgtcgcat aatgttggat aaaatgagct cgcattcggc gaggatgcgg 40740gcggatgaac aaatcgccca gccttagggg agggcaccaa agatgacagc ggtcttttga 40800tgctccttgc gttgagcggc cgcctcttcc gcctcgtgaa ggccggcctg cgcggtagtc 40860atcgttaata ggcttgtcgc ctgtacattt tgaatcattg cgtcatggat ctgcttgaga 40920agcaaaccat tggtcacggt tgcctgcatg atattgcgag atcgggaaag ctgagcagac 40980gtatcagcat tcgccgtcaa gcgtttgtcc atcgtttcca gattgtcagc cgcaatgcca 41040gcgctgtttg cggaaccggt gatctgcgat cgcaacaggt ccgcttcagc atcactaccc 41100acgactgcac gatctgtatc gctggtgatc gcacgtgccg tggtcgacat tggcattcgc 41160ggcgaaaaca tttcattgtc taggtccttc gtcgaaggat actgattttt ctggttgagc 41220gaagtcagta gtccagtaac gccgtaggcc gacgtcaaca tcgtaaccat cgctatagtc 41280tgagtgagat tctccgcagt cgcgagcgca gtcgcgagcg tctcagcctc cgttgccggg 41340tcgctaacaa caaactgcgc ccgcgcgggc tgaatatata gaaagctgca ggtcaaaact 41400gttgcaataa gttgcgtcgt cttcatcgtt tcctacctta tcaatcttct gcctcgtggt 41460gacgggccat gaattcgctg agccagccag atgagttgcc ttcttgtgcc tcgcgtagtc 41520gagttgcaaa gcgcaccgtg ttggcacgcc ccgaaagcac ggcgacatat tcacgcatat 41580cccgcagatc aaattcgcag atgacgcttc cactttctcg tttaagaaga aacttacggc 41640tgccgaccgt catgtcttca cggatcgcct gaaattcctt ttcggtacat ttcagtccat 41700cgacataagc cgatcgatct gcggttggtg atggatagaa aatcttcgtc atacattgcg 41760caaccaagct ggctcctagc ggcgattcca gaacatgctc tggttgctgc gttgccagta 41820ttagcatccc gttgtttttt cgaacggtca ggaggaattt gtcgacgaca gtcgaaaatt 41880tagggtttaa caaataggcg cgaaactcat cgcagctcat cacaaaacgg cggccgtcga 41940tcatggctcc aatccgatgc aggagatatg ctgcagcggg agcgcatact tcctcgtatt 42000cgagaagatg cgtcatgtcg aagccggtaa tcgacggatc taactttact tcgtcaactt 42060cgccgtcaaa tgcccagcca agcgcatggc cccggcacca gcgttggagc cgcgctcctg 42120cgccttcggc gggcccatgc aacaaaaatt cacgtaaccc cgcgattgaa cgcatttgtg 42180gatcaaacga gagctgacga tggataccac ggaccagacg gcggttctct tccggagaaa 42240tcccaccccg accatcactc tcgatgagag ccacgatcca ttcgcgcaga aaatcgtgtg 42300aggctgctgt gttttctagg ccacgcaacg gcgccaaccc gctgggtgtg cctctgtgaa 42360gtgccaaata tgttcctcct gtggcgcgaa ccagcaattc gccaccccgg tccttgtcaa 42420agaacacgac cgtacctgca cggtcgacca tgctctgttc gagcatggct agaacaaaca 42480tcatgagcgt cgtcttaccc ctcccgatag gcccgaatat tgccgtcatg ccaacatcgt 42540gctcatgcgg gatatagtcg aaaggcgttc cgccattggt acgaaatcgg gcaatcgcgt 42600tgccccagtg gcctgagctg gcgccctctg gaaagttttc gaaagagaca aaccctgcga 42660aattgcgtga agtgattgcg ccagggcgtg tgcgccactt aaaattcccc ggcaattggg 42720accaataggc cgcttccata ccaatacctt cttggacaac cacggcacct gcatccgcca 42780ttcgtgtccg agcccgcgcg cccctgtccc caagactatt gagatcgtct gcatagacgc 42840aaaggctcaa atgatgtgag cccataacga attcgttgct cgcaagtgcg tcctcagcct 42900cggataattt gccgatttga gtcacggctt tatcgccgga actcagcatc tggctcgatt 42960tgaggctaag tttcgcgtgc gcttgcgggc gagtcaggaa cgaaaaactc tgcgtgagaa 43020caagtggaaa atcgagggat agcagcgcgt tgagcatgcc cggccgtgtt tttgcagggt 43080attcgcgaaa cgaatagatg gatccaacgt aactgtcttt tggcgttctg atctcgagtc 43140ctcgcttgcc gcaaatgact ctgtcggtat aaatcgaagc gccgagtgag ccgctgacga 43200ccggaaccgg tgtgaaccga ccagtcatga tcaaccgtag cgcttcgcca atttcggtga 43260agagcacacc ctgcttctcg cggatgccaa gacgatgcag gccatacgct ttaagagagc 43320cagcgacaac atgccaaaga tcttccatgt tcctgatctg gcccgtgaga tcgttttccc 43380tttttccgct tagcttggtg aacctcctct ttaccttccc taaagccgcc tgtgggtaga 43440caatcaacgt aaggaagtgt tcattgcgga ggagttggcc ggagagcacg cgctgttcaa 43500aagcttcgtt caggctagcg gcgaaaacac tacggaagtg tcgcggcgcc gatgatggca 43560cgtcggcatg acgtacgagg tgagcatata ttgacacatg atcatcagcg atattgcgca 43620acagcgtgtt gaacgcacga caacgcgcat tgcgcatttc agtttcctca agctcgaatg 43680caacgccatc aattctcgca atggtcatga tcgatccgtc ttcaagaagg acgatatggt 43740cgctgaggtg gccaatataa gggagataga tctcaccgga tctttcggtc gttccactcg 43800cgccgagcat cacaccattc ctctccctcg tgggggaacc ctaattggat ttgggctaac 43860agtagcgccc ccccaaactg cactatcaat gcttcttccc gcggtccgca aaaatagcag 43920gacgacgctc gccgcattgt agtctcgctc cacgatgagc cgggctgcaa accataacgg 43980cacgagaacg acttcgtaga gcgggttctg aacgataacg atgacaaagc cggcgaacat 44040catgaataac cctgccaatg tcagtggcac cccaagaaac aatgcgggcc gtgtggctgc 44100gaggtaaagg gtcgattctt ccaaacgatc agccatcaac taccgccagt gagcgtttgg 44160ccgaggaagc tcgccccaaa catgataaca atgccgccga cgacgccggc aaccagccca 44220agcgaagccc gcccgaacat ccaggagatc ccgatagcga caatgccgag aacagcgagt 44280gactggccga acggaccaag gataaacgtg catatattgt taaccattgt ggcggggtca 44340gtgccgccac ccgcagattg cgctgcggcg ggtccggatg aggaaatgct ccatgcaatt 44400gcaccgcaca agcttggggc gcagctcgat atcacgcgca tcatcgcatt cgagagcgag 44460aggcgattta gatgtaaacg gtatctctca aagcatcgca tcaatgcgca cctccttagt 44520ataagtcgaa taagacttga ttgtcgtctg cggatttgcc gttgtcctgg tgtggcggtg 44580gcggagcgat taaaccgcca gcgccatcct cctgcgagcg gcgctgatat gacccccaaa 44640catcccacgt ctcttcggat tttagcgcct cgtgatcgtc ttttggaggc tcgattaacg 44700cgggcaccag cgattgagca gctgtttcaa cttttcgcac gtagccgttt gcaaaaccgc 44760cgatgaaatt accggtgttg taagcggaga tcgcccgacg aagcgcaaat tgcttctcgt 44820caatcgtttc gccgcctgca taacgacttt tcagcatgtt tgcagcggca gataatgatg 44880tgcacgcctg gagcgcaccg tcaggtgtca gaccgagcat agaaaaattt cgagagttta 44940tttgcatgag gccaacatcc agcgaatgcc gtgcatcgag acggtgcctg acgacttggg 45000ttgcttggct gtgatcttgc cagtgaagcg tttcgccggt cgtgttgtca tgaatcgcta 45060aaggatcaaa gcgactctcc accttagcta tcgccgcaag cgtagatgtc gcaactgatg 45120gggcacactt gcgagcaaca tggtcaaact cagcagatga gagtggcgtg gcaaggctcg 45180acgaacagaa ggagaccatc aaggcaagag aaagcgaccc cgatctctta agcatacctt 45240atctccttag ctcgcaacta acaccgcctc tcccgttgga agaagtgcgt tgttttatgt 45300tgaagattat cgggagggtc ggttactcga aaattttcaa ttgcttcttt atgatttcaa 45360ttgaagcgag aaacctcgcc cggcgtcttg gaacgcaaca tggaccgaga accgcgcatc 45420catgactaag caaccggatc gacctattca ggccgcagtt ggtcaggtca ggctcagaac 45480gaaaatgctc ggcgaggtta cgctgtctgt aaacccattc gatgaacggg aagcttcctt 45540ccgattgctc ttggcaggaa tattggccca tgcctgcttg cgctttgcaa atgctcttat 45600cgcgttggta tcatatgcct tgtccgccag cagaaacgca ctctaagcga ttatttgtaa 45660aaatgtttcg gtcatgcggc ggtcatgggc ttgacccgct gtcagcgcaa gacggatcgg 45720tcaaccgtcg gcatcgacaa cagcgtgaat cttggtggtc aaaccgccac gggaacgtcc 45780catacagcca tcgtcttgat cccgctgttt cccgtcgccg catgttggtg gacgcggaca 45840caggaactgt caatcatgac gacattctat cgaaagcctt ggaaatcaca ctcagaatat 45900gatcccagac gtctgcctca cgccatcgta caaagcgatt gtagcaggtt gtacaggaac 45960cgtatcgatc aggaacgtct gcccagggcg ggcccgtccg gaagcgccac aagatgacat 46020tgatcacccg cgtcaacgcg cggcacgcga cgcggcttat ttgggaacaa aggactgaac 46080aacagtccat tcgaaatcgg tgacatcaaa gcggggacgg gttatcagtg gcctccaagt 46140caagcctcaa tgaatcaaaa tcagaccgat ttgcaaacct gatttatgag tgtgcggcct 46200aaatgatgaa atcgtccttc tagatcgcct ccgtggtgta gcaacacctc gcagtatcgc 46260cgtgctgacc ttggccaggg aattgactgg caagggtgct ttcacatgac cgctcttttg 46320gccgcgatag atgatttcgt tgctgctttg ggcacgtaga aggagagaag tcatatcgga 46380gaaattcctc ctggcgcgag agcctgctct atcgcgacgg catcccactg tcgggaacag 46440accggatcat tcacgaggcg aaagtcgtca acacatgcgt tataggcatc ttcccttgaa 46500ggatgatctt gttgctgcca atctggaggt gcggcagccg caggcagatg cgatctcagc 46560gcaacttgcg gcaaaacatc tcactcacct gaaaaccact agcgagtctc gcgatcagac 46620gaaggccttt tacttaacga cacaatatcc gatgtctgca tcacaggcgt cgctatccca 46680gtcaatacta aagcggtgca ggaactaaag attactgatg acttaggcgt gccacgaggc 46740ctgagacgac gcgcgtagac agttttttga aatcattatc aaagtgatgg cctccgctga 46800agcctatcac ctctgcgccg gtctgtcgga gagatgggca agcattatta cggtcttcgc 46860gcccgtacat gcattggacg attgcagggt caatggatct gagatcatcc agaggattgc 46920cgcccttacc ttccgtttcg agttggagcc agcccctaaa tgagacgaca tagtcgactt 46980gatgtgacaa tgccaagaga gagatttgct taacccgatt tttttgctca agcgtaagcc 47040tattgaagct tgccggcatg acgtccgcgc cgaaagaata tcctacaagt aaaacattct 47100gcacaccgaa atgcttggtg tagacatcga ttatgtgacc aagatcctta gcagtttcgc 47160ttggggaccg ctccgaccag aaataccgaa gtgaactgac gccaatgaca ggaatccctt 47220ccgtctgcag ataggtacca tcgatagatc tgctgcctcg cgcgtttcgg tgatgacggt 47280gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 47340gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 47400atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 47460agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 47520aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 47580ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 47640gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 47700aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 47760gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 47820ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 47880cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 47940cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 48000gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 48060cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 48120agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 48180ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 48240ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 48300gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 48360cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 48420attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 48480accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 48540ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 48600gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 48660agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 48720ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 48780ttgttgccat tgctgcaggg gggggggggg ggggggactt ccattgttca ttccacggac 48840aaaaacagag aaaggaaacg acagaggcca aaaagcctcg ctttcagcac ctgtcgtttc 48900ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa 48960cgccttaaac cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac 49020ctgtcggatc accggaaagg acccgtaaag tgataatgat tatcatctac atatcacaac 49080gtgcgtggag gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat 49140tgatctgcat caacttaacg taaaaacaac ttcagacaat acaaatcagc gacactgaat 49200acggggcaac ctcatgtccc cccccccccc ccccctgcag gcatcgtggt gtcacgctcg 49260tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 49320cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 49380ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 49440ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 49500tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 49560agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 49620atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 49680gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 49740aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 49800tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 49860aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 49920gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 49980cttcaagaat tggtcgacga tcttgctgcg ttcggatatt ttcgtggagt tcccgccaca 50040gacccggatt gaaggcgaga tccagcaact cgcgccagat catcctgtga cggaactttg

50100gcgcgtgatg actggccagg acgtcggccg aaagagcgac aagcagatca cgcttttcga 50160cagcgtcgga tttgcgatcg aggatttttc ggcgctgcgc tacgtccgcg accgcgttga 50220gggatcaagc cacagcagcc cactcgacct tctagccgac ccagacgagc caagggatct 50280ttttggaatg ctgctccgtc gtcaggcttt ccgacgtttg ggtggttgaa cagaagtcat 50340tatcgtacgg aatgccaagc actcccgagg ggaaccctgt ggttggcatg cacatacaaa 50400tggacgaacg gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc 50460ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg 50520cgggaaacga caatctgatc atgagcggag aattaaggga gtcacgttat gacccccgcc 50580gatgacgcgg gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc 50640cactcagcaa gctggtacga ttgtaatacg actcactata gggcgaattg agcgctgttt 50700aaacgctctt caactggaag agcggttact accggttaag tgactagggt c 50751720DNAArtificial SequenceForward primer for GUS expression 7cggaagcaac gcgtaaactc 20821DNAArtificial Sequencereverse primer for GUS expression 8tgtgagcgtc gcagaacatt a 21920DNAArtificial SequenceProbe sequence 9cgcgtccgat cacctgcgtc 201023DNAArtificial SequenceTERM 2.1F primer 10ctgtcagttc caaacgtaaa acg 231125DNAArtificial SequenceTERM 2.1R primer 11aatctgatca tgagcggaga attaa 251220DNAArtificial SequenceTERM 1F primer 12tcccgggtcc ttaggaagac 201321DNAArtificial SequenceTERM 1R primer 13tggattcagc aggcctagaa g 211418DNAArtificial SequenceTERM 1P-probe 14tcctcaggat ttaaatgg 181520DNAArtificial SequenceActin_Fwd primer 15cttcgaatgc ccagcaatgt 201621DNAArtificial SequenceActin _rev primer 16gttcgcccac tagcgtacaa c 211715DNAArtificial SequenceActin _probe 17tcgaggctgt tcttt 1518460DNASorghum bicolor 18aactatctat actgtaataa tgttgtatag ccgccggata gctagctagt tagtcattca 60gcggcgatgg gtaataataa agtgtcatcc atccatcacc atgggtggca acgtgagcaa 120tgacctgatt gaacaaattg aaatgaaaag aagaaatatg ttatatgtca acgagatttc 180ctcataatgc cactgacaac gtgtgtccaa gaaatgtatc agtgatacgt atattcacaa 240tttttttatg acttatactc acaatttgtt tttttactac ttatactcga acaatttgtt 300gtgggtacca taacaatttc gatcgaatat atatcagaaa gttgacgaaa gtaagctcac 360tcaaaaagtt aaatgggctg cggaagctgc gtcaggccca agttttggct attctatccg 420gtatccacga ttttgatggc tgagggacat atgttcggct 46019461DNAArtificial SequenceConsensus Sequence for FIG. 6A-6Cmisc_feature(52)..(52)n is a, c, g, or tmisc_feature(290)..(291)n is a, c, g, or tmisc_feature(459)..(460)n is a, c, g, or t 19aactatctat actgtaataa tgttgtatag ccgccggata gctagctagt tnagtcattc 60agcggcgatg ggtaataata aagtgtcatc catccatcac catgggtggc aacgtgagca 120atgacctgat tgaacaaatt gaaatgaaaa gaagaaatat gttatatgtc aacgagattt 180cctcataatg ccactgacaa cgtgtgtcca agaaatgtat cagtgatacg tatattcaca 240atttttttat gacttatact cacaatttgt ttttttacta cttatactcn nacaatttgt 300tgtgggtacc ataacaattt cgatcgaata tatatcagaa agttgacgaa agtaagctca 360ctcaaaaagt taaatgggct gcggaagctg cgtcaggccc aagttttggc tattctatcc 420ggtatccacg attttgatgg ctgagggaca tatgttcgnn t 461

Claims

1. A recombinant construct comprising a polynucleotide sequence operably linked to a heterologous polynucleotide sequence, wherein the polynucleotide sequence comprises: (a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or SEQ ID NO:18; (b) a nucleotide sequence comprising a sequence with at least 95% identity to the sequence set forth in SEQ ID NO:1 or SEQ ID NO:18; or (c) a nucleotide sequence comprising a functional fragment of either (a) or (b); wherein the polynucleotide sequence functions as a transcriptional terminator in a plant cell.

2. The recombinant construct of claim 1 wherein the polynucleotide is operably linked to a promoter.

3. A plant comprising the recombinant construct of claim 1.

4. The plant of claim 3 wherein the plant is a monocot.

5. The plant of claim 4 wherein the plant is a maize plant.

6. A seed comprising the recombinant construct of claim 1.

7. The seed of claim 6 wherein the seed is from a monocot plant.

8. The seed of claim 7 wherein the seed is from a maize plant.

9. A method of expressing a heterologous polynucleotide in a plant, comprising the steps of: (a) introducing into a regenerable plant cell the recombinant construct of claim 2; (b) regenerating a transgenic plant from the regenerable plant cell of step (a), wherein the transgenic plant comprises the recombinant construct of claim 2; and (c) obtaining a progeny plant from the transgenic plant of step (b), wherein the progeny plant comprises the recombinant construct of claim 2 and exhibits expression of the heterologous polynucleotide.

10. The method of claim 9, wherein the plant is a monocot plant.

11. The method of claim 10, wherein the plant is a maize plant.

12. A plant comprising the recombinant construct of claim 2.

13. The plant of claim 12 wherein the plant is a monocot.

14. The plant of claim 13 wherein the plant is a maize plant.

15. A seed comprising the recombinant construct of claim 2.

16. The seed of claim 15 wherein the seed is from a monocot plant.

17. The seed of claim 16 wherein the seed is from a maize plant.