Plasmodium falciparum antigens

Abstract

The invention relates to antigens, associated with sterile immunity, and methods of their use, in an immunogenic formulation to confer an immune response against Plasmodium falciparum. The inventive antigens were identified by their association with sterile immunity against malaria.

Description

[0001] This application is a continuation of U.S. application Ser. No. 14/027,536, filed Sep. 16, 2013, which is a Divisional Application of Ser. No. 13/426,768, filed Mar. 22, 2012, with claims the benefit of U.S. Provisional Application No. 61/467,517, filed Mar. 25, 2011, which are incorporated herein by reference.

BACKGROUND OF INVENTION

[0002] 1. Field of Invention

[0003] The inventive subject matter relates to DNA sequences and polypeptides from Plasmodium falciparum for use as an anti-malaria vaccine component and methods of inducing an immune response to these antigens.

[0004] 2. Background Art

[0005] Malaria is caused by the vector borne organism Plasmodium spp. The parasite has a complex lifecycle requiring stage specific expression of proteins. These proteins can be expressed at different stages or be specific to stages. Malaria is an extremely important disease, with over 3 billion people living in malaria endemic areas. Over 1 million deaths are attributable to malaria per year. The emergence of drug resistant strains has compounded the problem of treating the disease. Unfortunately, no FDA-approved vaccine exists.

[0006] The entire genomic sequence of P. falciparum has been sequenced (Bowman et al., Nature, 400: 532-538 (1999), Gardner, et al., Nature, 419: 498-511 (2002)). The rodent malaria parasite, P. yoelii has also been sequenced (Carlton et al., Nature, 419: 512-519 (2002)). Despite this, however, the development of efficacious anti-malaria vaccines has been severely hampered by the paucity of promising antigens. Sequencing of the Plasmodium falciparum and Plasmodium yoelii genomes yielding identification over 5,200 genes in the genome. However, despite the large number of potential gene targets, use of the data set alone will not likely result in new vaccine constructs. Consequently, only 0.2% of the P. falciparum proteome is undergoing clinical testing. Moreover, these vaccine candidate antigens have failed to induce significant protection in volunteers. Nevertheless, immunization of mice and humans with radiation-attenuated sporozoites results in a high-grade immunity (>90%), suggesting that development of effective anti-malaria vaccines are possible. This protective immunity appears to target multiple sporozoite and liver stage antigens.

SUMMARY OF THE INVENTION

[0007] The invention relates to a vaccine composition and method of immunizing against Plasmodium falciparum. The inventive composition comprises P. falciparum liver-stage proteins associated with sterile protection against infectious P. falciparum. In one embodiment, the proteins can be incorporated into an immunogenic composition, singly or in multiple combinations, as subunit antigens. In another embodiment, for maximal immunogenicity, all identified proteins are included in a single immunogenic composition. Alternatively, multiple combinations of the proteins are administered in an immunization regimen through more than one immunogenic composition, each combination containing a specific combination of said immunogenic proteins. In one embodiment, the immunogenic composition comprises one of the identified proteins that has been isolated and purified. In another embodiment, the immunogenic composition comprises two or more, and up to all 19, of the identified proteins that have been purified and isolated.

[0008] In another embodiment, DNA encoding one or more of these proteins can be incorporated into vectors suitable for in vivo expression in a mammalian host. The expressed and purified proteins can then be administered, in one or multiple doses, to a mammal, such as humans. In this embodiment, DNA encoding one or more of the sterile-immunity associated proteins can be inserted into suitable expression systems. Suitable expression systems include, but are not limited to, adenoviral based systems, such as in Bruder, et al (patent application publication number US 20080248060, published Oct. 9, 2008) or a DNA plasmid system. In this embodiment, other vector systems include the DNA encoding P. falciparum is administered as an insert of the suitable expression system and expressed in vivo. In this embodiment, an immunogenic composition can comprise DNA encoding one or more, or all, of the sterile-immune associated proteins. The proteins can be expressed by a single vector encoding one or more of the proteins or by multiple expression systems suitable for expression of DNA in a mammal.

BRIEF DESCRIPTION OF DRAWINGS

[0009] FIG. 1. Summary of selection criteria and AUC profile. (A) Analyses were conducted to identify antigens that are associated with sporozoite-induced protection. After spot quantification, the signal intensity was a sin h/vsn log transformed to control variance, scaled by the negative control, post-immunization (post-I) and pre-challenge (pre-C) time points combined, and then analyzed according to defined statistical (Bayes-regularized t-tests, area under the receiver operating characteristics curve (AUC) analysis) and biological criteria. A total of 86 fragments (78 P. falciparum proteins) were identified by either approach through the global irradiated sporozoite list. Sterile immunity-associated proteins were in common to both approaches. (B) AUC values of all antigens for the not protected and protected cohorts were determined by R statistical environment software (available through: www.r-project.org). Antigen rank is plotted relative to their AUC value. An AUC value approaching 1.0 suggests a very strong association of an antigen to protection induced by irradiated sporozoite immunization; an AUC value of 0.5 indicates pure chance. An AUC value of 0.7 was chosen as a threshold for positivity.

[0010] FIG. 2. Magnitude and frequency of recognition of the proteins in Table 1 associated with irradiated sporozoite induced protection. For the antigens (ranked by AUC), the cumulative signal intensity representing the sum of signal intensities for each antigen by all subjects from protected or not protected groups are presented. *** P<0.0055.

[0011] FIG. 3. Magnitude and frequency of recognition of the proteins in Table 1 associated with irradiated sporozoite induced protection. Frequency of recognition of proteins by protected (P) and not protected (NP) individuals. # represents current clinical candidates: AMA1 (PF11_0344); CSP (PFC0210c); SSP2/TRAP (PF13_0201).

[0012] FIG. 4. Magnitude and frequency of recognition of the proteins in Table 1 associated with irradiated sporozoite induced protection. Average signal intensities of the antigens for each clinical group (protected, white bar; not protected, black bar) are presented as histograms, with antigen IDs listed on the x-axis. The average signal intensity (.+-.s.e.m) for each antigen is shown. Antigens are ordered by decreasing AUC value.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

[0013] As used, herein, "sterile immunity" refers to immunity, whereby the causative agent of the targeted disease causing organism is eliminated or inhibited from causing disease.

[0014] As used herein, the term "polypeptide" refers to a polymer of amino acids and does not refer to a specific length of the product. Proteins are included within the definition of polypeptides. As used herein, the proteins may be prepared for inclusion of an effective amount of one or more polypeptides described herein into an immunogenic composition by a number of means. For example, they may be included by first expressing the appropriate gene fragments by molecular methods, expression from plasmids or other expression systems such as viral systems and then isolated.

[0015] As used herein, "immunogenic fragments" of proteins refers to regions of proteins at least 8 amino acids in length, with the amino acid sequence derived from said protein, the fragment being capable of inducing an immune response or that is recognized by immune cells.

[0016] As used herein, "derivatives" of a protein is where a protein has more than 80% amino acid sequence identity to the sequences described herein. In this context, the term "identity" refers to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues that are the same, when aligned for maximum correspondence. Where sequences differ in conservative substitutions, i.e., substitution of residues with identical properties, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution.

[0017] As used herein, an aspect of the invention relates to nucleotide sequences that encode all or a substantial portion of the amino acid sequence encoding identified proteins or substantial portions, thereof. A "substantial portion" of a protein comprises enough of the amino acid sequence to afford putative identification of the protein the sequence encodes (Altschul, et al., J. Mol. Biol. 215: 403-410 (1993)). Furthermore, in general, this is approximately nine or more contiguous amino acids can lead to identification of the protein as homologous to a known protein. "Orthologous" nucleotide sequences are sequences encoding for proteins of the same function but in different species.

[0018] "Antigen" is a chemical moiety containing at least one epitope capable of stimulating or reacting with immune products, such as antibody or T-cells. An "immunogenic composition" refers to a chemical, compound or formulation that, once administered, will elicit an immune response; A "vaccine" is an immunogenic composition used to induce protective immunity; A "DNA expression system" is a molecular system containing plasmid or closed loop DNA containing elements for expressing an inserted DNA sequence as polypeptide; A "viral expression system" is any viral based system, including viral-like particles or viral replicons, containing elements for expressing an inserted DNA sequence as a polypeptide.

Example 1

Identification of Sterilely-Immune Associated Proteins

[0019] This example illustrates the identification of proteins associated with sterile immune against malaria. In this example, volunteers, experimentally immunized with radiation attenuated P. falciparum sporozoites (Irrspz), were clinically divergent after challenge with infectious P. falciparum sporozoites; six individuals were sterilely-protected and were classified as sporozoite-immune (IrrSpz protected) and five individuals developed blood stage parasitemia and were classified as sporozoite-exposed but non-immune (IrrSpz not protected).

[0020] Subjects were experimentally immunized with radiation-attenuated P. falciparum, (Pf(3D7)), sporozoites and challenged with P. falciparum-infected Anopheline mosquitoes, as described previously (Hoffman, et al., J. Infect. Dis. 185:1155-1164 (2002); Egan, et al., Am. J. Trop. Med. Hyg. 49:166-173 (1993)). Subjects were monitored daily, post-challenge, by thin blood smears to determine if they developed blood stage malaria. A complete absence of blood stage parasitemia during the 28 day follow up was considered sterile protection.

[0021] Six sporozoite-immunized volunteers were protected against sporozoite challenge and five were not protected (i.e. developed clinical malaria). One individual is represented in both groups since he was not protected in the initial challenge but was after a second series of immunizations. Plasma was collected from each individual before immunization (pre-immunization), post third immunization, at the completion of the immunization series (5.sub.th, 6.sub.th, or 7.sup.th immunization), immediately prior to challenge (pre-challenge) and following challenge (post-challenge). An infectivity control group (n=3) was simultaneously infected with the same P. falciparum-infected mosquitoes used for challenge to demonstrate parasite infectivity; plasma was collected from these individuals at corresponding time points before (pre-challenge) and after (post-challenge) challenge. An additional group (n=5) were mock immunized by the bite of non-infected mosquitoes and plasma was collected at the time points corresponding to pre-immunization, post-third immunization and post last immunization time points of the IrrSpz immunized subjects. Plasma collected from volunteers with no known history of malaria exposure (n=10) was also evaluated.

[0022] To identify antigens associated with IrrSpz-induced protection, plasma collected from protected, not protected, infectivity, mock immunized and naive individuals at different stages of the immunization process (or corresponding time points for the non-immunized individuals) were probed on P. falciparum microarrays against 2,320 fragments, representing 1,200 P. falciparum proteins. Antibody recognition of each fragment was then assessed.

[0023] There were distinct antibody profiles for each immunization group, with variability in responses between individuals within all groups. Overall, a markedly different pattern in antibody recognition was apparent between the protected and not protected groups, consistent with previous data (Doolan, et al, Proteomics 8:4680-4694). There was no difference in antibody reactivity at pre-challenge and post-challenge time points for protected individuals who did not develop blood stage parasitemia or clinical disease (P<0.6) and no change in the number of antigens recognized (340 pre-challenge vs. 380 post-challenge).

[0024] Putative proteins, and protein and DNA sequences, were derived from the P. falciparum genomic sequence database (www.plasmodb.org) and selected based on stage-specific transcription or protein expression, subcellular localization, secondary protein structure, and documented immunogenicity in humans or animal models; this list included all putative P. falciparum proteins with evidence of expression at some point during the parasite life cycle by MudPIT (multidimensional protein identification technology) (Florens, et al., Nature 419:520-526 (2002)), www.plasmoDB.org) at the time of antigen selection (n=1049).

[0025] Selected genes were amplified by polymerase chain reaction (PCR) amplification of P. falciparum genomic DNA (3D7 strain) using custom PCR primers that included homologous cloning sites to the pXT7 plasmid (Davies, et al., Proc. Natl. Acad. Sci (USA) 102:547-552 (2005)). Due to restrictions in producing long PCR products, proteins with exons longer than 3000 bp were divided into multiple overlapping sections, with 50 nucleotide overlaps. PCR reactions were carried out in a 50 .mu.l reaction volume containing 1-10 ng of P. falciparum genomic DNA (gDNA) (3D7 strain), 0.04 U/.mu.l proofreading Taq polymerase (Triplemaster.TM., Eppendorf, Hauppauge, N.Y.), and 0.4 mM each dNTPs, with the following cycling conditions: 95.degree. C. for 3 min; 35 cycles of 95.degree. C. for 15 s, 40.degree. C. for 30 s and 50.degree. C. for 60 s/kb; and a final extension of 50.degree. C. for 10 min. For some proteins that proved difficult to amplify, 50 ng of gDNA was used. Products were visualized by agarose gel electrophoresis, and quantified by fluorometry (Picogreen.TM., Molecular Probes.RTM., Invitrogen, Carlsbad, Calif.).

[0026] Plasmids were created from the PCR amplified fragments using in vitro recombination cloning and the pXT7 cloning vector, which encodes an N-terminal 10.times. Histidine (His) and C-terminal Haemagglutinin (HA) tag (3.2 kb, KanR). Briefly, 1 ng of Bam HI, digested, linearized pXT7 template and custom primers 5'-CTACCCATACGATGTTCCGGATTAC and 5'-CTCGAGCATATGCTTGTCGTCGTCG were used to generate a linear acceptor vector containing the target gene by PCR (50 .mu.l reaction) with 0.02 U/.mu.l Taq polymerase, 0.1 mg/ml gelatine (Porcine), and 0.2 mM each dNTPs. The following cycling conditions were used: 95.degree. C. for 5 min; 30 cycles of 95.degree. C. for 0.5 min, 50.degree. C. for 0.5 min and 72.degree. C. for 3.5 min; and a final extension of 72.degree. C. for 10 min. After purification (Qiagen, Valencia, Calif.), PCR products were visualized by gel electrophoresis, and quantified by fluorometry (Picogreen.TM., Molecular Probes.RTM., Invitrogen, Carlsbad, Calif.).

[0027] Open reading frames (ORFs) were cloned into the linearized pXT7 plasmid by a recombination reaction as previously described (Davies, et al., Proc. Natl. Acad. Sci. (USA) 102:547-552 (2005). Briefly, a 20 .mu.l mixture of linear vector and PCR-generated ORF fragment at a 1:1 molar ratio between vector and insert was transformed into DH5.alpha. competent cells without further purification and incubated for 1 h at 37.degree. C., before dilution into an overnight culture of 3 ml LB broth containing Kanamycin 50 .mu.g/ml. Plasmids were isolated and purified using the QIAprep.TM. spin miniprep, (Qiagen, Valencia, Calif.) without further selection. A subset of these plasmids was sequence confirmed.

[0028] Protein expression and detection was conducted using E. coli in vitro cell-free transcription and translation reactions (rapid translation system (RTS) 100 E. coli HY kits, Roche). Reactions were carried out in 25 .mu.l volumes with a 5-hour incubation at 30.degree. C., according to manufacturer's instructions. For quality control purposes, relative protein expression efficiency for approximately 31% of all ORFs was assessed by immunodot blots by spotting 0.3 .mu.l of the RTS reaction on nitrocellulose (NC) and air drying before blocking in 5% nonfat milk powder in TBS containing 0.05% polysorbate 20. Dot blots were stained with mouse anti-polyHIS mAb (clone HIS-1; Sigma) and rat anti-HA mAb (clone 3F10) (F. Hoffmann-La Roche Ltd, Basel, Switzerland) and detected with alkaline phosphatase-conjugated goat anti-mouse IgG (H+L) or goat anti-rat IgG (H+L) secondary antibodies respectively or with human hyperimmune plasma (diluted 1:1000 in blocking buffer with 10% E. coli lysate) followed by alkaline phosphatase-conjugated goat anti-human IgG secondary antibody (H+L). Blots were visualized with nitroblue tetrazolium (NBT) developer.

[0029] Microchips were prepared by mixing 15 .mu.l of RTS reaction with 10 .mu.l of 0.125% polysorbate 20/PBS, and then 15 .mu.l volumes were transferred to 384 well plates. Plates were centrifuged (1600.times.g, 5 min) to pellet any precipitates and proteins in the supernatant were immediately printed without further purification onto 3-pad NC-coated FAST.TM. glass slides (Schleicher & Schuell, Bioscience, Inc., Keene, N.H.) using an OmniGrid 100 microarray printer (Genomic Solutions, Ann Arbour, Mich.). Arrays were allowed to dry and stored away from light at room temperature in a desiccator.

[0030] RTS reactions carried out in the absence of DNA plasmids were printed on each array as negative or non-differentially recognized control spots. Purified human total IgG and Epstein-Barr nuclear antigen 1 (EBNA1) protein were also printed in serially diluted concentrations on each array, as probing and plasma controls respectively.

[0031] Since high titers of anti-E. coli antibodies present in the human plasma could mask protein-specific reactivity in the arrays, plasma were pre-absorbed against E. coli lysate in protein array blocking buffer (Schleicher & Schuell) (1:100 dilution) for 30 minutes at room temperature (Davis, et al., Proc. Natl. Acad. Sci. (USA) 102:547-552 (2005).

[0032] Slides were rehydrated and blocked in blocking buffer for 30 min at room temperature. Then 500 .mu.l plasma diluted 1:100 in blocking buffer was added to each pad and slides were incubated overnight at 4.degree. C. with a gentle constant speed on a platform rocker (Ratek.TM., InVitro Technologies). Serum antibodies were detected with biotin-conjugated goat anti-human IgG secondary antibody (1:1000 dilution, 1 h at room temperature with gentle constant speed, Fc fragment, (Jackson ImmunoResearch Laboratories, Inc, West Grove, Pa.) and visualized with a streptavidin P-3-conjugated antibody (1:200 dilution, 1 h at room temperature with gentle constant speed. Air dried slides were scanned on an Axon GenePix.TM. 4300A array scanner (Molecular Devices, CA) and fluorescence intensities quantified using the Axon GenePix Pro 7.TM. software (Molecular Devices, Sunnyvale, Calif.). Using the above software, all signal intensities were corrected for spot-specific background, where the background value for a spot was calculated from a region surrounding the spot.

[0033] To validate the P. falciparum protein microarrays, antibody reactivity to three well characterized malaria vaccine candidates, CSP, AMA1 and merozoite surface protein 2 (MSP2), were expressed via the RTS system or using traditional methods and were printed on the same protein microarray chip. There was a high degree of correlation between reactivity to the proteins produced by the two methods, (CSP (r=0.77; P<0.001), AMA1 (r=-0.78; P<0.001), MSP2 (r=0.96; P<0.001)). To examine the reproducibility of the array probing, the reactivity against all HA spots from two independent chips was assessed and was highly correlated (r=0.92, P<0.001). Additionally, a smaller microarray chip was fabricated with a subset of 49 P. falciparum proteins and probed with the same plasma used to probe the larger 2320 fragment P. falciparum protein microarray. There was a strong correlation between plasma reactivity on the two microarrays (r=0.91, P<0.001).

[0034] In order to analyze low and high signal intensities (differential recognition) using standard statistical methods, the heteroskedastic nature of microarray platforms (Durbin, et al., Bioinformatics 18 Suppl. 1:S105-110 (2002); Ideker, et al., J. Comput. Biol 7:805-817 (2000) and inherent variance-mean dependence in the data (Sundaresh, et al., Bioinformatics 22:1760-1766 (2006); Sundaresh, et al., Bioinformatics 23:i508-518 (2007)) needed to be considered and stabilized. Raw signal intensities were therefore variant log transformed by using either a sin h (Excel 2007, Microsoft; (Sundaresh, et al., Bioinformatics 22:1760-1766 (2006)) or variance stabilizing normalization (vsn) (Bioconductor software (www.bioconductor.org) (Huber, et al., Bioinformatics 18suppl. 1:S96-104 (2002)) transformation, to reduce the variance and experimental effects in the raw signal between differentially recognized antigens.

[0035] Before ranking and selection of antigens, data for each plasma sample was scaled by multiplication to a normalization ratio (average SI of all negative controls for that individual/average SI of all negative controls for all individuals) to apply the same baseline for all plasma. The post-immunization and pre-challenge time points for each individual in each group were combined for sample size purposes, since data showed no significant differences between those time points, and data was biologically and statistically analyzed. Area under receiver operating characteristic curves (ROC) (AUC) and Bayesian regularized t-tests (R statistical environment software, www.rproject.org) were used to identify differentially recognized antigens between the protected and not protected groups (Baldi and Long, Bioinformatics 17:509-519 (2001)) (FIG. 1). Please note that the statistical testing (both AUC and p-values) have not under gone multiple testing correction, rather these statistics were used as a method to compare the two groups and rank the data accordingly. The identification of antigens of interest was made on the combination of the rankings in addition to other relevant criteria i.e. recognition in different groups.

[0036] The criterion for positive immunoreactivity to a given protein for both transformation methods was determined as an average signal intensity two standard deviation above the negative control (all individuals). Data were visually presented as a heatmap where colors represent the SI range for that antigen. Red represents high SI (reactivity), black represents intermediate reactivity and green represents low reactivity. Gene ontology (GO) annotation analysis was performed using the GOstatsand org.Pfplasmo.db R packages. The significance of annotation enrichment was assessed using Fisher's exact test.

[0037] A marked change in seroreactivity was noted at pre-challenge vs. post-challenge time points for the not protected individuals where there was a significant increase in overall signal intensity (SI) (P<2e-91) and number of antigens recognized (292 pre-challenge vs. 739 post-challenge), consistent with exposure to blood stage antigens upon development of patent parasitemia.

[0038] The profile for the infectivity control individuals also showed a significant increase in SI pre-challenge vs. post-challenge (P<2e-30) to a subset of antigens (289 prechallenge vs. 546 post-challenge) and 89% (546) of the antigens recognized post-challenge were also recognized by the not protected group post-challenge, and therefore appear to be expressed by the blood stage parasite. Minimal reactivity to P. falciparum antigens was noted with plasma from malaria naive individuals or mock immunized individuals, consistent with a lack of exposure to P. falciparum.

[0039] To identify P. falciparum antigens putatively correlated with protection against sporozoite challenge, normalization and variance correction were performed using either the vsn and a sin h methods. Since there were no significant changes in SI or number of antigens recognized at post-3' immunization or post-last immunization (prechallenge) time points for both protected and not protected groups [P<0.25 (not protected), P<0.2 (protected)], data from these biologically similar time points were combined for subsequent analyses. CyberT.TM. (Institute for Genomics and Bioinformatics, University of California, Irvine, Calif.) (a Bayesian regularized t-test) p-values and a receiver operating characteristic (ROC) curve of the mean SI were used to measure the power of an antigen to discriminate between protected and not protected cohorts, thus identifying antigens putatively associated with sporozoite-induced protection. The selection criteria are depicted in FIG. 1. An area under the ROC curve (AUC) value of 1.0 is a perfect prediction, 0.5 is a random chance, and <0.5 is a prediction in the wrong direction.

[0040] For both vsn and a sin h approaches, antigens were selected according to the Following criteria: (i) antigenic (protected); i.e. average SI greater than two standard deviations (SD) above negative control; (ii) recognized by at least two protected individuals; (iii) average SI greater in the protected group than not protected group; (iv) an AUC value equal to or greater than 0.7; and (v) CyberT.TM. p-value <0.05 (protected vs. not protected).

[0041] Combining the antigens identified by either approach revealed a total of 86 fragments representing 77 proteins, termed "global IrrSpz" list. Five proteins (PF14_0051, PFI0240c, PF10_0183, PF10_0211, MAL13P1.107) have two immunoreactive fragments on this list and two proteins (PF11_0395, MAL7P1.146) have three fragments, since open reading frames (ORFs)>3000 bp were cloned as overlapping segments. When ranked by AUC values, five antigens on this list (6%) have an AUC value greater than 0.9 (1.5e-3<P>3.8e-3), and 31 antigens (36%) have an AUC greater than 0.8 (1.5e-3<P>4.43e-2).

[0042] The global IrrSpz list included three current vaccine candidates: AMA1 (PFI11_0344), CSP (PFC0210c), and SSP2/TRAP (PF13_0201). Interestingly, these were highly antigenic in protected individuals, with 92% (AMA1), 100% (CSP) and 100% (SSP2/TRAP) recognition within this cohort, but were also recognized to a similar extent by not protected individuals (92%, 100%, 100%, respectively). The other 75 proteins on this list have not been previously characterized.

[0043] A subset of 20 fragments representing 19 proteins was common to both vsn and a sin h approaches. These are shown in Table 1. Three of these antigens (i.e., PFB0285c, PFE1085w, PF08_0054) were not recognized by not protected individuals and 10 of the antigens had a higher frequency of recognition in the protected cohort as compared with the not protected cohort. Seven antigens (including CSP, SSP2/TRAP, and AMA1) were recognized at a similar frequency by the two cohorts but the protected individuals had a significantly higher magnitude of response (0.0034<P>0.0077, 2-4 fold higher average SI) (FIG. 2).

TABLE-US-00001 TABLE 1 Characteristics of antigens associated with sporozoite-induced protection. Fold P value Average signal enrich Frequency of (Protected intensity (Protected recognition Total Product v not Not v not Not # Gene ID Description Details AUC Protected) Protected Protected Protected) Protected Protected exons PFE10825w gamma- exon 1 0.917 1.67E-03 3324 1293 3 100% 40% 1 glutamycysteine synthetase PFB0285c condensed exon 1 0.917 2.77E-03 1341 337 4 67% 0% 1 Plasmodium protein, unknown function PF14_0051 DNA mismatch exon 4 0.888 1.52E-03 2784 553 5 67% 30% 4 repair protein, putative PF00465w condensed complete 0.852 7.68E-03 12669 3205 4 100% 90% 1 Plasmodium protein, unknown function PF11620w asperagineaspartate exon 1 0.833 1.06E-02 2471 807 3 92% 50% 3 rich protein, putative PF10_0211 .sctn. condensed exon 1 0.833 4.43E-02 1752 853 2 100% 40% 5 Plasmodium membrane protein, unknown function PF10_0211 .sctn. condensed exon 2 0.833 4.43E-02 1752 853 2 63% 40% 5 Plasmodium membrane protein, unknown function PFB0150c protein kinase, exon 2 0.824 2.37E-02 2528 620 4 50% 40% 1 putative PF11_0344 AMA1 complete 0.824 2.88E-02 4455 2248 2 92% 90% 1 PFE0060w PFESP2 erythrocyte exon 2 0.815 9.87E-03 5625 3030 2 100% 30% 2 surface protein PF08_0034 histone exon 1 0.815 3.36E-02 2480 1293 2 92% 100% 4 acetyltransferase GO45, putative PF08_0054 heat shock 70 kDa complete 0.806 1.52E-02 1214 221 5 25% 0% 1 protein PFI2140c ADP ribosylation exon 1 0.806 2.16E-02 3738 965 4 58% 40% 1 factor GTPase-activating protein PFC0210c CSP complete 0.800 1.72E-02 15118 10066 2 100% 100% 1 PFE1085w DEAD-box exon 1 0.796 1.03E-02 1201 203 6 42% 0% 1 subfamily ATP-dependent helicase, putative PF11_0404 transcription factor exon 2 0.787 3.42E-02 2907 1542 2 100% 90% 3 with AP2 domain(s), putative PF13_0201 SSP2/TRAP complete 0.769 3.11E-02 29578 9618 3 100% 100% 1 PFI2505c rhopty neck protein exon 8 0.759 1.57E-02 4011 1531 3 100% 60% 8 3, putative PF13_0222 phosphatase exon 1 0.750 4.98E-02 4243 960 4 58% 30% 1 putative MAL13P1.22 DNA ligase 1 exon 2 0.713 3.91E-02 1739 466 4 50% 10% 2 Presence in # # genomid Functional Mw TM PEXEL/ Exported Signal proteomic categories Gene ID bp aa (kDa) pf domains motif protein peptide datasets (plasma08) PFE10825w 3192 1063 124.46 5.1 0 0 no no yes Protein Synthesis PFB0285c 4311 1436 164.85 10.04 0 0 no no yes Hypothetical PF14_0051 4548 1515 179.83 8.9 0 1 no yes yes Cell Cycle (DNA processing) PF00465w 1728 575 68.53 9.42 0 0 no no yes Hypothetical PF11620w 16320 5439 646.34 6.38 0 1 no no yes Protein Synthesis PF10_0211 .sctn. 20605 6834 830.20 9.51 5 4 no no yes Hypothetical PF10_0211 .sctn. 20605 6834 830.20 9.51 5 4 no no yes Protein Synthesis PFB0150c 7458 2485 293.77 7.21 0 0 no no yes Hypothetical PF11_0344 1869 622 72.04 5.23 1 0 no yes yes Cell Surface (Apical organties) PFE0060w 1227 406 48.72 7.19 3 1 yes yes no Hypothetical PF08_0034 4368 1465 170.92 6.65 0 0 no no yes Metabolism PF08_0054 2034 677 73.92 5.33 0 0 no no yes Cell Cycle (DNA processing) PFI2140c 999 332 37.29 5.42 0 0 no no yes Hypothetical PFC0210c 1194 397 42.65 5.18 1 2 no yes yes Vintence PFE1085w 2526 841 97.34 7.96 0 3 no no yes Cell Cycle (DNA processing) PF11_0404 7962 2653 309.45 6.12 0 0 no no yes Transcription PF13_0201 1725 574 64.74 4.7 0 1 no yes yes Hypothetical PFI2505c 6648 2215 263.16 9.62 3 0 no yes yes Hypothetical PF13_0222 1728 575 67.56 5.06 0 0 no no no Protein Synthesis MAL13P1.22 2739 912 104.51 7.66 0 1 no yes yes Cell Cycle (DNA processing)

[0044] The 10 antigens on the global list with the highest AUC values (0.86-0.93) also had very low p values (protected vs. not protected; 0.0015-0.033). Unexpectedly, all of these antigens had an average SI below 3000 (protected), suggesting that high antibody recognition and reactivity (i.e., serodominance) to a single antigen does not correlate with sporozoite-induced protection. Indeed, the average SI for 75 of the 86 antigens was below 5000, and 80 of the 86 were below 10000.

[0045] As shown in FIG. 2, protected individuals had a significantly greater cumulative antibody response to the antigens of Table 1, as compared to not protected individuals (P<0.0055). FIG. 4 shows the average signal intensities of the 20 antigens in Table 1 for each clinical group. This remarkable difference in cumulative response between the protected and non-protected individuals, suggests that sterile immunity is associated with a panel of antigens, not with individual antigens.

[0046] The majority of the global IrrSpz list (93%; 72/77 proteins) and genes in Table 1 (89%, 17/19 proteins) were detected via independent mass spectrometry (MS/MS) analysis of sporozoites (Florens, et al., Nature 419:520-526 (2002); Hall, et al., Science 307:82-86 (2005); Lasonder, et al., PLoS Pathog. 4:e1000195 (2008); Kappe, et al., Proc. Natl. Acad. Sci (USA) 98:9895-9900 (2001)) or liver stage and/or sporozoite gene expression data (Kappe, et al., Proc. Natl. Acad. Sci (USA) 98:9895-9900 (2001); Le Roch, et al., Science 301:1503-1508 (2003); Rosinski-Chupin, et al., BMC Genomics 8:466 (2007); Siau, et al., PLoS Pathog. 4:e1000121 (2008); Tarun, et al., Proc. Natl. Acad. Sci. (USA) 105:305-310 (2008)), PlasmoDB.TM.), validating the expression of the genes during the pre-erythrocytic stage of the parasite life cycle.

Example 2

Characterization of Antigens Associated with Protection

[0047] This example illustrates the characterization of the proteins associated with protection. To identify specific genomic or proteomic features of antigens putatively associated with IrrSpz-induced protection, selected characteristics of the global IrrSpz list and the proteins listed in Table 1 were compared to the Plasmodium falciparum genome and to the known blood stage antigens (BSA) and sporozoite/liver stage antigens (SLA). The antigens from the global IrrSpz list and genes listed in Table 1 average respectively 3 and 2.5 fold larger for their transcription length, protein length and molecular weight as compared to the Plasmodium falciparum genome, BSA or SLA (Table 2); this is consistent with the fact that large proteins potentially present a greater number of B cell epitopes for antibody recognition. There was no difference in average isoelectric points (pI) or the number of exons compared to the P. falciparum genome, although the BSA and SLA had a lower average pI value than the other groups (Table 2).

TABLE-US-00002 TABLE 2 Comparison of genomic and proteomic features from antigens associated with protection.sup.1 Known sporozoite/ Known Top 20 Global liver stage blood stage Pf list list antigens antigens genome (n = 20) (n = 86) (n = 7) (n = 17) (n = 5479) Ave number 2.3 3 1.6 1.9 2.5 of Exons Ave Tran- 5711 6909 2245 2274 2270 scription Length (bp) Ave protein 1902 2302 747 760 755 length (aa) Ave pI 7.1 7.8 5.3 5.3 8 value Ave Mw 224 272 85 87 89 (kDa) Number 30% 34% 71% 41% 31% with TM domains Number 35% 24% 100% 65% 19% with Signal peptide Number 45% 53% 71% 23% 27% with PEXEL motif .sup.1Characteristics of the global irradiated sporozoite list and top 20 list (i.e., antigens in Table 1) are compared to known vaccine candidates and the P. falciparum genome (PlasmoDB .TM.). Attributes such as average (Ave) number of exons, gene (bp) and protein (aa) size, isoelectric point (pI), presence of features that are recognized by antibodies (transmembrane domains, signal peptide, (PEXEL motif), were compared.

[0048] An enrichment of Plasmodium export element (PEXEL) motifs was noted in a global IrrSpz list (2-fold, Table 2) compared to whole genome and known BSA. The global list of revealed 86 fragments representing 77 proteins.

[0049] PEXEL motifs are found in five of seven known SLA, consistent with a function in the transport of liver stage parasite proteins to hepatocytes. Although signal peptides have been identified in all known SLA and 11 of 17 known BSA, consistent with a role for secretion in inducing antibody responses, antigens containing a signal peptide were not enriched in the proteins in Table 1 (35%, 7/20, p-value=0.08) or global list (24%, 21/68, p-value=0.31). These data are consistent with the concept that IrrSpz protection is mediated by T cells rather than antibodies. Also consistent with this is the observation that only 30% (p-value=0.64) and 34% (p-value=0.9) of genes in Table 1 or Global IrrSpz list antigens, respectively, had defined transmembrane domains, which are associated with exposure of surfaces for antibody recognition (Table 2).

[0050] Identified proteins were assigned functional categories based on annotation information available from PlasmoDB (PlasmoDB: a functional genomic database for malaria parasites. Nucleic Acids Res. 2008 Oct. 31. Aurrecoechea C, et al). Fifty-six percent of the global and 40% of the 20 sterile immunity-associated proteins were hypothetical proteins, with the remainder assigned to multiple functional categories. Interestingly, the functional categories for the proteins in Table 1 showed a notable concentration of genes/proteins involved in or regulating DNA processing/cell cycle (20%) and protein synthesis (20%); cellular communication, transport facilitation and protein fate were not represented in this subset. Gene ontology analysis also indicates an overrepresentation of biological processes involving DNA, invasion and metabolic processes, DNA related activity and perhaps a localization of these proteins for invasion purposes.

Example 3

Immunogenic Composition and Method of Use for Immunizing Against Malaria Using Identified Proteins

[0051] This example illustrates a method of inducing an immune response against malaria. In a preferred embodiment, one or more of the identified proteins listed in Table 1, associated with sterile immunity, can be incorporated in an immunogenic composition, such as a vaccine. In one example, all of the proteins listed in Table 1 can be incorporated into an immunogenic composition.

[0052] In this embodiment, the immunogenic composition can be a subunit immunogenic composition or vaccine, composed of one or more of the isolated proteins, or immunogenic fragments or derivatives of the proteins, selected from the list of proteins in Table 1 that afforded sterile protection against malaria. Alternatively, an immunogenic composition or vaccine can be composed of nucleic acid, encoding one or more of the proteins of Table 1, or immunogenic fragments or derivatives of the proteins, inserted into one or more expression systems suitable for expression in mammals, such as humans. Table 3 lists the proteins from Table 1 and their associated sequence identification numbers.

TABLE-US-00003 TABLE 3 Summary of Sequence Numbers Protein Sequence ID No. SEQ ID. No. Designation (DNA) (Polypeptide) PF10925w 1 2 PFB0285c 3 4 PF14_0051 5 6 PFD0485w 7 8 PFL1620w 9 10 PF10_0211 11 12 PFB0150c 13 14 PF11_0344 15 16 PFE0060w 17 18 PF08_0034 19 20 PF08_0054 21 22 PFL2140c 23 24 PFC0210c 25 26 PFE1085w 27 28 PF11_0404 29 30 PF13_0201 31 32 PFL2505c 33 34 PF13_0222 35 36 MAL13P1.22 37 38

[0053] It is contemplated that the antigen(s) can be expressed either as a component of a DNA vaccine or other platform system. An example of a contemplated expression system includes, but is not limited to, viral systems, including replicating and nonreplicating vectors. Examples of contemplated viral vectors include adenovirus, alphavirus, poxvirus, cytopmegalovirus, canine distemper virus and yellow fever virus. The antigen(s) could be incorporated as an insert of a DNA or other vaccine expression system, either as a single antigen or multiple antigen expression systems from a single or multiple promoters.

[0054] The contemplated invention includes a method for inducing an immune response in mammals, including humans. In this example, antigen(s), either as polypeptide or incorporated into a nucleic acid expression system, such as a DNA or viral system, are administered in one or more doses. The method also contemplates inducing an immune response utilizing a prime-boost immunization regimen. In this embodiment, one or more priming immunization doses would be administered followed by one or more boosting immunizations.

[0055] The priming and boosting immunization comprises a composition containing a malaria polypeptide, wherein the polypeptide contains one or more of the polypeptide sequences of Table 3, or immunogenic derivatives, thereof. Alternatively, the immunogenic composition can comprise an expression system capable of expressing the polypeptides in mammals. In this embodiment, nucleic acid molecules, listed in Table 3 or encoding the polypeptides, or derivatives thereof, of Table 3 can be inserted into a DNA plasmid or a viral expression vectors. Examples of viral expression vector systems include: alphavirus (and alphavirus replicons), adenovirus, poxvirus, adeno-associated virus, cytomegalovirus, canine distemper virus, yellow fever virus and retrovirus.

[0056] The contemplated methods also include immunization regimens wherein the priming immunization comprises malarial peptides expressed from a DNA plasmid expression vector or an adenovirus, while the boosting immunization includes malaria peptides expressed from either: adenovirus, adenovirus that is heterologous to the priming adenovirus, poxvirus or one or more malaria polypeptides. The expressed malaria polypeptide and encoding nucleic acid can be any of a number of malarial polypeptides and nucleic acid sequences Table 3, or immunogenic derivatives of the polypeptides, thereof.

Example 4

Blood Stage Specific Antigens

[0057] A strong immune response to all well characterized blood stage antigens was also seen post-challenge in a cohort that was not afford protection. As expected, negligible responses to antigens known to be expressed at the blood stage were observed in the protected cohort and no changes in their heatmap profile occurred throughout the study time course.

[0058] Analysis of the responses post challenge for the not protected cohort allowed identification of novel blood stage specific P. falciparum antigens, using the following criteria: (i) antigenic; i.e. average SI greater than two SDs above negative control in at least two not protected individuals; (ii) statistically significant recognition (p) after challenge as compared to pre challenge <0.05 (not protected); and (iii) not recognized by protected subjects.

[0059] As illustrated in Table 4, a total of 23 antigens met these criteria, of which two have been previously characterized (MSP1 [PFI1475w] and liver stage antigen 3 [LSA3, PFB0915w]) and 21 are novel. The sequence identification numbers of the proteins in Table 4 and the DNA encoding these proteins are shown in Table 5.

TABLE-US-00004 TABLE 4 Irradiated sporozoite and Present Present in naturally iRBC Present orthologue P. Naturally acquired MS Merozoite yoelii blood acquired immunity (Florens, MS stage immunity study et al., (Florens, et (Watanabe, et (Crompton, (Doolan, et Protein Gene ID (2004)) al., (2002)) al, (2007)) et al., (2010)) al., (2008)) description PHI0855w No No No Yes No Hypo. Protein PE14_0198 Yes Yes Yes No No Gly. tRNA ligase (putative) PFA0535c No No Yes No No Kinesin (putative) PF08_0018 No No Yes No No Translation initiation factor-like protein PFL1705w No No Yes No No RNA binding protein (putative) MAL6P1.146 No No No No No P. falciparum PK4 protein kinase PFL0410w No YES YES NO NO Cys. Repeat modular protein 1 PFL2440w No No Yes No No DNA repair protein rbp 16 (putative) PFL2520w No No Yes No No Reticulocyte binding protein (pseudouene) PF14_0084 No Yes Yes No No Hypothetical protein PFI1500w No No Yes No No Hypothetical protein PF14_0327 No No Yes No No Methionine aminopeptidase, type II PF11_0270 Yes Yes No Yes No Threonine-tRNA ligase (putative) PF11_0479 No Yes Yes No No Hypothetical PFI0755c Yes Yes Yes No No 6-phosphofructokinase (putative) PE11_0091 No Yes Yes No No Transcription factor (putative) PF14_0315 No Yes Yes No No Hypothetical protein MAL7P1.137 No No Yes No No Kelch protein (putative) PFI1510w No No Yes No No Nucleolar protein Nop 52 (putative) PFI1475w Yes Yes Yes No Yes MSP1 PFB0915w No Yes No No Yes LSA3 PF10_0323 Yes Yes No No No Hypothetical protein MAL8P1.23 No Yes Yes No No Ubiquitin-protein ligase 1 (putative)

TABLE-US-00005 TABLE 5 SEQ ID No. Gene ID (DNA/Protein) PFI0855 39/40 PF14_0198 41/42 PFA0535c 43/44 PF08_0018 45/46 PFL1705w 47/48 MAL6P1.146 49/50 PFL0410w 51/52 PFL2440w 53/54 PFL 2520w 55/56 PF14_0084 57/58 PFI1500w 59/60 PF14_0327 61/62 PF11_0270 63/64 PF11_0479 65/66 PFI0755c 67/68 PF11_0091 69/70 PF14_0315 71/72 MAL7P1.137 73/74 PFI1510w 75/76 PFI1475w 77/78 PFB0915w 79/80 PF10_0323 81/82 MAL8P1.23 83/84

[0060] For 22 of the 23 antigens, evidence of their expression at the blood stage has been independently reported from data sets of P. falciparum infected red blood cell MS/MS (Florens, et al., Mol. Biochem. Parasitol. 135:1-11(2004)), P. falciparum merozoite MS/MS (Florens, et al., Nature 419:520-526 (2002)), P. yoelii 17XNL asexual blood stage EST datasets (P. falciparum orthologs) (Watanabe, et al., Nucleic Acids Res. 35:D431-438 (2007)) and previous P. falciparum protein microarrays which analyzed plasma from individuals with blood stage malaria (Crompton, et al., PNAS 107:6958-6963 (2010); Doolan, et al., Proteomics, 8:4680-4694 (2008)). Four antigens, expressed in both the blood stage and the sporozoite/liver stage of the parasite life cycle, were also identified.

[0061] In a preferred embodiment, one or more of the proteins of Table 4, the sequence identification numbers of which are shown in Table 5, or immunogenic fragments or derivatives of these proteins can be incorporated into an immunogenic composition. Alternatively, the immunogenic composition can comprise DNA encoding the proteins, fragments or derivatives. In this embodiment, DNA encoding the proteins of Table 4, the sequence identification numbers of which are shown in Table 5, can be in one or more expression vectors capable of expression in mammalian hosts. As a further embodiment, one or more of the proteins of Table 4, immunogenic fragments or derivatives, or DNA encoding these proteins, can be incorporated into immunogenic an immunogenic composition with one or more of the proteins of Table 3, or immunogenic fragments or derivatives of these proteins, or DNA encoding these proteins.

[0062] It is contemplated that the antigens can be expressed either as a component of a DNA vaccine, or other platform system. An example of a contemplated expression system includes, but is not limited to, viral systems, including replicating and nonreplicating vectors. Examples of contemplated viral vectors include adenovirus, alphavirus, poxvirus, cytopmegalovirus, canine distemper virus and yellow fever virus. The antigen could be incorporated as an insert of a DNA or other vaccine expression system, either as a single antigen or multiple antigen expression systems from a single or multiple promoters.

[0063] Another embodiment is a method for inducing an immune response in mammals, including humans utilizing the expressed proteins listed in Table 4. The sequence listings corresponding to the DNA sequences of the genes, and their protein expression products, are listed in Table 5. In this embodiment, antigens, either as polypeptide or incorporated into a nucleic acid expression system, such as a DNA or viral system, are administered in one or more doses. The method also contemplates inducing an immune response utilizing a prime-boost immunization regimen. In this embodiment, one or more priming immunization doses would be administered followed by one or more boosting immunizations.

[0064] The priming and boosting immunization comprises a composition containing a malaria polypeptide, wherein the polypeptide contains one or more of the polypeptide sequences of Table 5, or immunogenic derivatives, thereof. Alternatively, the immunogenic composition can comprise an expression system capable of expressing the polypeptides in mammals. In this embodiment, nucleic acid molecules, listed in Table 5 or encoding the polypeptides, or derivatives thereof, of Table Scan be inserted into a DNA plasmid or a viral expression vectors. Examples of viral expression vector systems include: alphavirus (and alphavirus replicons), adenovirus, poxvirus, adeno-associated virus, cytomegalovirus, canine distemper virus, yellow fever virus and retrovirus.

[0065] The contemplated methods also include immunization regimens wherein the priming immunization comprises malarial peptides expressed from a DNA plasmid expression vector or an adenovirus, while the boosting immunization includes malaria peptides expressed from either: adenovirus, adenovirus that is heterologous to the priming adenovirus, poxvirus or one or more malaria polypeptides. The expressed malaria polypeptide and encoding nucleic acid can be any of a number of malarial polypeptides and nucleic acid sequences shown in Table 5, or immunogenic derivatives of the polypeptides, thereof.

[0066] All references, including publications, patent applications and patents, cited are herein incorporated by reference.

[0067] Having described the invention, one of skill in the art will appreciate in the appended claims that many modifications and variations of the present invention are possible in light of the above teachings. It is therefore, to be understood that, within the scope of the appended claims, the invention may be practiced otherwise than as specifically described.

Sequence CWU 1

1

3813192DNAPlasmodium falciparum 1atgggttttc taaaaatcgg aacgccatta agctgggatg atgtacaaga tgtgaaatct 60ttaattagat tatatggtat attacagttt gtacatgtat ataaattaaa taaagaccgt 120tatgatgaaa atataatgtt tggtgatgaa attgaatata taataataag aaatgatgaa 180agcttaaaag aatcgtctgc cttattatgt gcttctgatt taatagatga aatgatgaat 240ttagaaagtg ttattgactg tcaatatggt tcacattgga ctccagaata ttcttctttt 300actatagaag gtactccatc agtaccattt aaattagata ttaattcttc atgttttgtt 360gaagattgta tgagaattag aagaagtaaa ttaaataatg ttcttagtgc cgttcaagga 420gctagagcta ttacacttcc ttgttttcct aatgttcttt taaataatag tgttcttatg 480gctagaagaa ttactggcca tgaaagtaca aaaaagaaat ttgattcaaa aggaaaagtc 540gaatttatag aaaatgcgaa aattaaagaa aaggttcata attcaaataa taatatacac 600aaaataatta ataataaaaa caatgaatct aaaattgtta ataatgcatt tgatcaaaat 660aaaatctctt ctattgaaat ggtatcctat gagatggatg aaaataaatc taccaatttt 720gttaattcag atacagtatt tgcaaaaaat gacgaagaag gagaagtaga agaagaagac 780gaaaatgaaa acgaacaaca acaacaacag caacaatacc aatcgaattt acaacaacaa 840aatgtacaac caaaacaacg tcaacaaatg atacaatatg tctatgatga tgaaatagaa 900aataaaaata aagaaaagga taatacacca agaagctgta atgattacaa taatgttaat 960gatagttcaa atacacaaga tatatttatt agttcattaa aaaaaacaga ttcacttttc 1020gaatgtgaag tttttaaacc tgaacaaaca aataaatata gtaaaagtgc acttattaca 1080gatatgacta taagtcctca tgccagatat gttaccttaa cacaaaatat aagaaaacga 1140agaggaacca aaattgtatc ctttaatcct atatataaag atattaatac cgaaaaaatg 1200gatcactgga aaatgtcact tgattgtaat gataaaagac tttttaaaaa agttaagaag 1260aaactcacat tagatgaaca cctaatatgg aataaatcta tgacaaacaa aaagattata 1320gatagaaaaa ataacaatcc aagagatgag gtactaaaca cgtctaattt tacattagct 1380atgacaaata aagaagaaaa taataatgaa aatagcctta tggatagggt tttgaaaaat 1440agtttatttg cgaatatgga tgatgaaggt gattatattt atgtttataa tagagaattt 1500atacaagaat attcagaaaa atgtaaaaat ccaattaaaa attatgtata tttagatgct 1560atgttttttg gtatgagtat gtgttgtcaa caattaacca tgtcttttcc aactgttgat 1620gatgcaaaat atgtgtatga tcaactagcc gttattgcac cacttttcct tgctcttact 1680gcatgtactc cctatttagg tgggtttcta actgaaacag atacaagatg gagagtaata 1740tctaacagtg ttgattgtag aacggaagaa gaattatcat atatatgtaa accaagatat 1800tcaggaatat ccttatatat atccaatgag ttacctttaa aaagaaatta ctatttttat 1860aacgatgtag atgtaatact tgacaaaaat gtgtatgaca aattgaaaaa ggaaaacgtg 1920gatgaatatt tagctagaca tatagcatct ttatttgtaa gagatccaat tgttgttttt 1980gaaggttcct atagtgaacg tgacattgct acaattcaga agaaaataat ggaattgtca 2040aacgatgaag ataaaattaa aggtatggta gaagggtcat catcaaatag tttgtcatca 2100aaagttttgt tagacaatga ggataggaat aaaaatgata gtagtataaa agaaatgaat 2160aaatctaatg ataataacca taataataat tgccatattg aatataataa taaaaaagat 2220ataaatatga ataagatata tttaagtgat aattttgaat ttatagaaga ttatgaagaa 2280aaggttttat catctcatca acattttgaa aattttcaaa gtactaattg gaatagtgta 2340cgttttaagc cacctccaat tcttgataat cattttaaag gacctagttc aattggatgg 2400agagttgaat ttagaactcc tgatattcaa attaccgatt ttgagaattc ttgtgttgtt 2460acattagtta tgttattatc taaatttata ttaaaagaaa gattaaattt atatattcct 2520atgacattgt tagaagaaaa tttatttaga gcatccaaaa gagaagcttt aacaaaagaa 2580aaattttatt tccgtaaaga tctaagttat gataccttga ataatgaatt tgaggaaaaa 2640agtatatatg atatattttt taatgaaacc aatggattgt ttttcttatg ttacaaatat 2700gtggacgaac tatttaaaga aggattatta aatcaatcag ccaaaaataa aatagatgaa 2760tatattgaat tcgttaaaca aagatgtagt ggaaaaatat gtacaggtgc tatgtattta 2820agaaatttta tattaaatca tccagcttat gaaaaaaatt cttatatcaa tagtaaaatt 2880aattatgata tatgtaaatt aatagctgat attggaaagg gattaatcat accacaagaa 2940ttattaggtg tttttgtcga tccatataaa gaaaggataa aaagtgatat aaggcaaatt 3000aatgaaagcc aatacctgaa gtccttggca tataaatata tttcaggaga ggattatacg 3060caatacttgc tacttaatga agtacttaag gatgaccagg attattgtac atgtaccaga 3120cgtacaattt atgaggaatc tatggataat acagtggagt ttgcaaaaaa aatgtacgaa 3180ctgagtgcat aa 319221063PRTPlasmodium falciparum 2Met Gly Phe Leu Lys Ile Gly Thr Pro Leu Ser Trp Asp Asp Val Gln 1 5 10 15 Asp Val Lys Ser Leu Ile Arg Leu Tyr Gly Ile Leu Gln Phe Val His 20 25 30 Val Tyr Lys Leu Asn Lys Asp Arg Tyr Asp Glu Asn Ile Met Phe Gly 35 40 45 Asp Glu Ile Glu Tyr Ile Ile Ile Arg Asn Asp Glu Ser Leu Lys Glu 50 55 60 Ser Ser Ala Leu Leu Cys Ala Ser Asp Leu Ile Asp Glu Met Met Asn 65 70 75 80 Leu Glu Ser Val Ile Asp Cys Gln Tyr Gly Ser His Trp Thr Pro Glu 85 90 95 Tyr Ser Ser Phe Thr Ile Glu Gly Thr Pro Ser Val Pro Phe Lys Leu 100 105 110 Asp Ile Asn Ser Ser Cys Phe Val Glu Asp Cys Met Arg Ile Arg Arg 115 120 125 Ser Lys Leu Asn Asn Val Leu Ser Ala Val Gln Gly Ala Arg Ala Ile 130 135 140 Thr Leu Pro Cys Phe Pro Asn Val Leu Leu Asn Asn Ser Val Leu Met 145 150 155 160 Ala Arg Arg Ile Thr Gly His Glu Ser Thr Lys Lys Lys Phe Asp Ser 165 170 175 Lys Gly Lys Val Glu Phe Ile Glu Asn Ala Lys Ile Lys Glu Lys Val 180 185 190 His Asn Ser Asn Asn Asn Ile His Lys Ile Ile Asn Asn Lys Asn Asn 195 200 205 Glu Ser Lys Ile Val Asn Asn Ala Phe Asp Gln Asn Lys Ile Ser Ser 210 215 220 Ile Glu Met Val Ser Tyr Glu Met Asp Glu Asn Lys Ser Thr Asn Phe 225 230 235 240 Val Asn Ser Asp Thr Val Phe Ala Lys Asn Asp Glu Glu Gly Glu Val 245 250 255 Glu Glu Glu Asp Glu Asn Glu Asn Glu Gln Gln Gln Gln Gln Gln Gln 260 265 270 Tyr Gln Ser Asn Leu Gln Gln Gln Asn Val Gln Pro Lys Gln Arg Gln 275 280 285 Gln Met Ile Gln Tyr Val Tyr Asp Asp Glu Ile Glu Asn Lys Asn Lys 290 295 300 Glu Lys Asp Asn Thr Pro Arg Ser Cys Asn Asp Tyr Asn Asn Val Asn 305 310 315 320 Asp Ser Ser Asn Thr Gln Asp Ile Phe Ile Ser Ser Leu Lys Lys Thr 325 330 335 Asp Ser Leu Phe Glu Cys Glu Val Phe Lys Pro Glu Gln Thr Asn Lys 340 345 350 Tyr Ser Lys Ser Ala Leu Ile Thr Asp Met Thr Ile Ser Pro His Ala 355 360 365 Arg Tyr Val Thr Leu Thr Gln Asn Ile Arg Lys Arg Arg Gly Thr Lys 370 375 380 Ile Val Ser Phe Asn Pro Ile Tyr Lys Asp Ile Asn Thr Glu Lys Met 385 390 395 400 Asp His Trp Lys Met Ser Leu Asp Cys Asn Asp Lys Arg Leu Phe Lys 405 410 415 Lys Val Lys Lys Lys Leu Thr Leu Asp Glu His Leu Ile Trp Asn Lys 420 425 430 Ser Met Thr Asn Lys Lys Ile Ile Asp Arg Lys Asn Asn Asn Pro Arg 435 440 445 Asp Glu Val Leu Asn Thr Ser Asn Phe Thr Leu Ala Met Thr Asn Lys 450 455 460 Glu Glu Asn Asn Asn Glu Asn Ser Leu Met Asp Arg Val Leu Lys Asn 465 470 475 480 Ser Leu Phe Ala Asn Met Asp Asp Glu Gly Asp Tyr Ile Tyr Val Tyr 485 490 495 Asn Arg Glu Phe Ile Gln Glu Tyr Ser Glu Lys Cys Lys Asn Pro Ile 500 505 510 Lys Asn Tyr Val Tyr Leu Asp Ala Met Phe Phe Gly Met Ser Met Cys 515 520 525 Cys Gln Gln Leu Thr Met Ser Phe Pro Thr Val Asp Asp Ala Lys Tyr 530 535 540 Val Tyr Asp Gln Leu Ala Val Ile Ala Pro Leu Phe Leu Ala Leu Thr 545 550 555 560 Ala Cys Thr Pro Tyr Leu Gly Gly Phe Leu Thr Glu Thr Asp Thr Arg 565 570 575 Trp Arg Val Ile Ser Asn Ser Val Asp Cys Arg Thr Glu Glu Glu Leu 580 585 590 Ser Tyr Ile Cys Lys Pro Arg Tyr Ser Gly Ile Ser Leu Tyr Ile Ser 595 600 605 Asn Glu Leu Pro Leu Lys Arg Asn Tyr Tyr Phe Tyr Asn Asp Val Asp 610 615 620 Val Ile Leu Asp Lys Asn Val Tyr Asp Lys Leu Lys Lys Glu Asn Val 625 630 635 640 Asp Glu Tyr Leu Ala Arg His Ile Ala Ser Leu Phe Val Arg Asp Pro 645 650 655 Ile Val Val Phe Glu Gly Ser Tyr Ser Glu Arg Asp Ile Ala Thr Ile 660 665 670 Gln Lys Lys Ile Met Glu Leu Ser Asn Asp Glu Asp Lys Ile Lys Gly 675 680 685 Met Val Glu Gly Ser Ser Ser Asn Ser Leu Ser Ser Lys Val Leu Leu 690 695 700 Asp Asn Glu Asp Arg Asn Lys Asn Asp Ser Ser Ile Lys Glu Met Asn 705 710 715 720 Lys Ser Asn Asp Asn Asn His Asn Asn Asn Cys His Ile Glu Tyr Asn 725 730 735 Asn Lys Lys Asp Ile Asn Met Asn Lys Ile Tyr Leu Ser Asp Asn Phe 740 745 750 Glu Phe Ile Glu Asp Tyr Glu Glu Lys Val Leu Ser Ser His Gln His 755 760 765 Phe Glu Asn Phe Gln Ser Thr Asn Trp Asn Ser Val Arg Phe Lys Pro 770 775 780 Pro Pro Ile Leu Asp Asn His Phe Lys Gly Pro Ser Ser Ile Gly Trp 785 790 795 800 Arg Val Glu Phe Arg Thr Pro Asp Ile Gln Ile Thr Asp Phe Glu Asn 805 810 815 Ser Cys Val Val Thr Leu Val Met Leu Leu Ser Lys Phe Ile Leu Lys 820 825 830 Glu Arg Leu Asn Leu Tyr Ile Pro Met Thr Leu Leu Glu Glu Asn Leu 835 840 845 Phe Arg Ala Ser Lys Arg Glu Ala Leu Thr Lys Glu Lys Phe Tyr Phe 850 855 860 Arg Lys Asp Leu Ser Tyr Asp Thr Leu Asn Asn Glu Phe Glu Glu Lys 865 870 875 880 Ser Ile Tyr Asp Ile Phe Phe Asn Glu Thr Asn Gly Leu Phe Phe Leu 885 890 895 Cys Tyr Lys Tyr Val Asp Glu Leu Phe Lys Glu Gly Leu Leu Asn Gln 900 905 910 Ser Ala Lys Asn Lys Ile Asp Glu Tyr Ile Glu Phe Val Lys Gln Arg 915 920 925 Cys Ser Gly Lys Ile Cys Thr Gly Ala Met Tyr Leu Arg Asn Phe Ile 930 935 940 Leu Asn His Pro Ala Tyr Glu Lys Asn Ser Tyr Ile Asn Ser Lys Ile 945 950 955 960 Asn Tyr Asp Ile Cys Lys Leu Ile Ala Asp Ile Gly Lys Gly Leu Ile 965 970 975 Ile Pro Gln Glu Leu Leu Gly Val Phe Val Asp Pro Tyr Lys Glu Arg 980 985 990 Ile Lys Ser Asp Ile Arg Gln Ile Asn Glu Ser Gln Tyr Leu Lys Ser 995 1000 1005 Leu Ala Tyr Lys Tyr Ile Ser Gly Glu Asp Tyr Thr Gln Tyr Leu 1010 1015 1020 Leu Leu Asn Glu Val Leu Lys Asp Asp Gln Asp Tyr Cys Thr Cys 1025 1030 1035 Thr Arg Arg Thr Ile Tyr Glu Glu Ser Met Asp Asn Thr Val Glu 1040 1045 1050 Phe Ala Lys Lys Met Tyr Glu Leu Ser Ala 1055 1060 34311DNAPlasmodium falciparum 3atgcagcaag atggtaatat acaagtaaaa atattaaaag atgtaaatcc ttattattat 60aaaaaagaaa atccatacga caatttagaa tataataaat atgttatgaa tgtgaatgat 120gttgagggaa cgaatattat tgatgataaa aaaaaggatt taggtaaatc aaaatatgat 180atttttacga cagattcttt gtcaactact acagatgaag tatcttatag ttatcaaatc 240gaaaataaaa atgaagaaaa agaatatttg agatattatg ataaacaggg aggtataata 300cgacaagata ataataataa tgaaaataat aataataata tttgtaataa tgaccataat 360aataataaca tttgtaataa tgaaaatatg ttaacaacaa aaaaaaatga taatacaata 420ataaatagta atattaaata tttaaacaat aataatatat ttaataccaa catggtacca 480caaaaaaatc acacacaaat atttaacccc tatgataaaa gtatgaggaa tattcaattg 540tataataaag cagtaagctt tttaaaaaat gatggggaca taaattcaaa aaaaaatacc 600catgataatt taatgttcct aaaaaatata cgaagtaaaa gtaataataa tcttattgta 660aataggaaaa taacaaatca tgtgacaaat aatgtgataa gtggtatgac aaataaagtg 720ataggtggta tggcaagtgg tatgacaaat aatgtgacaa gtagtataac aaataatatg 780acaagtagta tgacaaataa tatggcaagt ggtatgacaa gtagtatgac aaataatatg 840gcaagtggta tgacaagtag tataacaaat aatatgacaa gtagtatgac aaataatatg 900gcaagtggta tgacaagtag tataacaaat aatatgacaa gtagtatgac aaataatatg 960gcaagtagta tgacaagtag tatgacaaat aatatgacaa gtagtatgac aaataatatg 1020acaagtagca tgacaaataa tatgcttaat aatatgaata gggttgtaac gaataatatt 1080attactaata tgaacagatc agtttcggga agtaaaagta ttaacatgag caatctgtta 1140atcataaata agatggatta tggtaatgac atttaccata acaacaacaa caataataat 1200aatagtagta gtggtagcaa tattgtcagt ggtaaatatt ttgtgaattc tcaaaatagt 1260agcaaaaata atttctttac aaaagtagga gaaagcacaa tacgatcacc aacaaacatt 1320ttagatattt ataaacaggg aaatatgtat atgcacatcc caaaaaatgc agatttaatg 1380aacaatgttt cttcatatag tatagcacat gaaaattata ttaaaaggga caacacaaat 1440gtgactcatg tgttaaataa taaccacctt gtaaatataa ataatgtggt aaacaacaac 1500aatttgaata ataacaacaa tttgaataat aacaacaatt tgaatagtaa caacaatttg 1560aatagtaaca acaatttgaa taacaacaat aatttaatta ataacaataa tttaattaac 1620aataattatg taagaaataa ccaagctgta aataacgctc atacattaaa tgctcatttt 1680aacaaaagcg ataatgtgga taacatgaga aaccatattc caaacaatga caataaaaat 1740attgtaaata tgttaaattt aaaaaatatg aaaagtatta atgatttgtc agttttaata 1800aataaaaata aacctattca tcatgtaata aatgggacag aggttcaaca aaaaagatcc 1860ttgagtaatg ttcaaaaatt aaaaacgttg aacacttttc caaatgccaa gggaaggttt 1920agtttgatca ataaaatggc tagcatgccg aatatgagca cgacgagtag tatgaacatg 1980tcagggttaa acacaagctc gtctgaagga ttaaccaaca taattaatat gaataacata 2040aacagtgtga ataatataaa cagtgtgaat aatataaaca gtgtgaataa tataaacagt 2100gtgaataatt taaacagtgt gaataatata aacagtgtga ataatataaa cagtgtgaat 2160aatataaaca gtgtgaataa cataaacagt gtgaataata taaacagtgt gaataatata 2220aacagtgtga ataatttaaa cagtgtgaat aatataaaca gtgtgaataa tataaacaat 2280ataaattata taaacaatat aaattatgta aatatgaaca agggtttgaa ccctataaat 2340aatgtaagca atataagcag cctaaaattg ttaaacaata ataatgatat aaaaaagaaa 2400tttaatacat atggaaaaag tgaggcgtcc gaaaatttaa gtaagaatgt caaatacata 2460aaatatattc aagaaaatat taaatatttg aataatctgg atgataataa aaggaagtat 2520tctctcacaa gtataaatga tgtgggatgt ataaaaaaaa aaaaaaatat gaatgattta 2580tttttaggaa agcatgataa tatgttacgt acagatgaaa taccaaagat aaatttagga 2640aaaaatatat tgaataataa taaaataata aattataatg ataatgataa aagtaatata 2700ataaataatg ttataaataa gaacatttct acagatttgg tgaatgatag agagggtgat 2760atgaataaaa tgaatattca taatagagag aaggatgaaa ataattatat aaatattggg 2820gataataaaa taaagaaaaa tcaaattgat gttgttaata ataaggtaat gaaattggat 2880aatatggagg atgaagaagc tatgaataaa ttatcattaa tatctttata tccaaacaat 2940aatcacatta ttaataatgt gaataatgtg aataatgtga ataatgtgaa taatgtaaat 3000aatgtgaata atgtaaataa tgtgaataat gtaaataatg tgaataatgt gaattatatg 3060aataatatga ataatgtgaa taatgtgaat aatatgaata atgtgaataa tatgaataat 3120gtgaataatg tgaatagtgt gaataatata aaaggaataa ataacatgaa caataataat 3180aataatataa acatgaatcg aagttataaa atgaacatga aaaaagtgtc caaaaaagat 3240aatggtcaaa atgtggtatc ggaaaaacga tttagcgaag aaaaatataa ttttcttaaa 3300aatttaatac gaaataataa gaatatggta aaattaaaat atctgaataa atttttagga 3360aaaagaagtg gaccatcaat aaaaaacaat atgaacgata tgatggttaa gatgaataat 3420aacatgaagg atattatgca tataaaggat gcaactaata taaacaaaat taataataag 3480ttggtaaatt taaatacgaa taattgtatt tcttataata gttgtaacaa aatgaattat 3540atacataaat gtaaaaagaa aagagtttta tgtttagata cgaaacatgg gaaaaatgaa 3600ataaaacaaa atgagaaatt aatttataca aattacgaaa taaaaatgtt tttgttaaat 3660acgattaaag caataggtat agtttttaag aaatggaaat ttaaaaattt cggattatat 3720ttttggtatc atattaaatg tatagaaaat gaaagagatt taaattttta tattaaaata 3780tttaattttt tgtttgaaat cataacaggg aaaaatatat attaccaaat aaatgacatt 3840cataacattg ttgcattatt taaagaattt aaaatatatg attgtaagca tgttcttaaa 3900aaaagtatta aagttttaaa taaatatgca aaaaaaaatt caaaggaatt ctcacttttt 3960gaaaataatc aacatgtagt attagacatc aataaacata tgttatttaa tgatgatgaa 4020aaaaaattaa caacttgtaa tataaaacaa aatgaacaag aacaaattaa aaccaaagtt 4080ctttatgatc atgataatat aaatgtagac acgaaacaaa attatcaaaa aataataaca 4140aataaaaata atcatccaaa ggataatttt tattcatatc tatatgattc cttacaagga 4200aaaaatcata tctttcaaca accaggcgta caaaatatgc atatatataa tatgtttgca 4260caatttaatg aattaaattt taatgatatg tttaactttt caataaccta a 431141436PRTPlasmodium falciparum 4Met Gln Gln Asp Gly Asn Ile Gln Val Lys Ile Leu Lys Asp Val Asn 1 5 10 15 Pro Tyr Tyr Tyr Lys Lys Glu Asn Pro Tyr Asp Asn Leu Glu Tyr Asn 20 25 30 Lys Tyr Val Met Asn Val Asn Asp Val Glu Gly Thr Asn Ile Ile Asp 35 40 45 Asp Lys Lys Lys Asp Leu Gly Lys Ser Lys Tyr Asp Ile Phe Thr Thr 50 55 60

Asp Ser Leu Ser Thr Thr Thr Asp Glu Val Ser Tyr Ser Tyr Gln Ile 65 70 75 80 Glu Asn Lys Asn Glu Glu Lys Glu Tyr Leu Arg Tyr Tyr Asp Lys Gln 85 90 95 Gly Gly Ile Ile Arg Gln Asp Asn Asn Asn Asn Glu Asn Asn Asn Asn 100 105 110 Asn Ile Cys Asn Asn Asp His Asn Asn Asn Asn Ile Cys Asn Asn Glu 115 120 125 Asn Met Leu Thr Thr Lys Lys Asn Asp Asn Thr Ile Ile Asn Ser Asn 130 135 140 Ile Lys Tyr Leu Asn Asn Asn Asn Ile Phe Asn Thr Asn Met Val Pro 145 150 155 160 Gln Lys Asn His Thr Gln Ile Phe Asn Pro Tyr Asp Lys Ser Met Arg 165 170 175 Asn Ile Gln Leu Tyr Asn Lys Ala Val Ser Phe Leu Lys Asn Asp Gly 180 185 190 Asp Ile Asn Ser Lys Lys Asn Thr His Asp Asn Leu Met Phe Leu Lys 195 200 205 Asn Ile Arg Ser Lys Ser Asn Asn Asn Leu Ile Val Asn Arg Lys Ile 210 215 220 Thr Asn His Val Thr Asn Asn Val Ile Ser Gly Met Thr Asn Lys Val 225 230 235 240 Ile Gly Gly Met Ala Ser Gly Met Thr Asn Asn Val Thr Ser Ser Ile 245 250 255 Thr Asn Asn Met Thr Ser Ser Met Thr Asn Asn Met Ala Ser Gly Met 260 265 270 Thr Ser Ser Met Thr Asn Asn Met Ala Ser Gly Met Thr Ser Ser Ile 275 280 285 Thr Asn Asn Met Thr Ser Ser Met Thr Asn Asn Met Ala Ser Gly Met 290 295 300 Thr Ser Ser Ile Thr Asn Asn Met Thr Ser Ser Met Thr Asn Asn Met 305 310 315 320 Ala Ser Ser Met Thr Ser Ser Met Thr Asn Asn Met Thr Ser Ser Met 325 330 335 Thr Asn Asn Met Thr Ser Ser Met Thr Asn Asn Met Leu Asn Asn Met 340 345 350 Asn Arg Val Val Thr Asn Asn Ile Ile Thr Asn Met Asn Arg Ser Val 355 360 365 Ser Gly Ser Lys Ser Ile Asn Met Ser Asn Leu Leu Ile Ile Asn Lys 370 375 380 Met Asp Tyr Gly Asn Asp Ile Tyr His Asn Asn Asn Asn Asn Asn Asn 385 390 395 400 Asn Ser Ser Ser Gly Ser Asn Ile Val Ser Gly Lys Tyr Phe Val Asn 405 410 415 Ser Gln Asn Ser Ser Lys Asn Asn Phe Phe Thr Lys Val Gly Glu Ser 420 425 430 Thr Ile Arg Ser Pro Thr Asn Ile Leu Asp Ile Tyr Lys Gln Gly Asn 435 440 445 Met Tyr Met His Ile Pro Lys Asn Ala Asp Leu Met Asn Asn Val Ser 450 455 460 Ser Tyr Ser Ile Ala His Glu Asn Tyr Ile Lys Arg Asp Asn Thr Asn 465 470 475 480 Val Thr His Val Leu Asn Asn Asn His Leu Val Asn Ile Asn Asn Val 485 490 495 Val Asn Asn Asn Asn Leu Asn Asn Asn Asn Asn Leu Asn Asn Asn Asn 500 505 510 Asn Leu Asn Ser Asn Asn Asn Leu Asn Ser Asn Asn Asn Leu Asn Asn 515 520 525 Asn Asn Asn Leu Ile Asn Asn Asn Asn Leu Ile Asn Asn Asn Tyr Val 530 535 540 Arg Asn Asn Gln Ala Val Asn Asn Ala His Thr Leu Asn Ala His Phe 545 550 555 560 Asn Lys Ser Asp Asn Val Asp Asn Met Arg Asn His Ile Pro Asn Asn 565 570 575 Asp Asn Lys Asn Ile Val Asn Met Leu Asn Leu Lys Asn Met Lys Ser 580 585 590 Ile Asn Asp Leu Ser Val Leu Ile Asn Lys Asn Lys Pro Ile His His 595 600 605 Val Ile Asn Gly Thr Glu Val Gln Gln Lys Arg Ser Leu Ser Asn Val 610 615 620 Gln Lys Leu Lys Thr Leu Asn Thr Phe Pro Asn Ala Lys Gly Arg Phe 625 630 635 640 Ser Leu Ile Asn Lys Met Ala Ser Met Pro Asn Met Ser Thr Thr Ser 645 650 655 Ser Met Asn Met Ser Gly Leu Asn Thr Ser Ser Ser Glu Gly Leu Thr 660 665 670 Asn Ile Ile Asn Met Asn Asn Ile Asn Ser Val Asn Asn Ile Asn Ser 675 680 685 Val Asn Asn Ile Asn Ser Val Asn Asn Ile Asn Ser Val Asn Asn Leu 690 695 700 Asn Ser Val Asn Asn Ile Asn Ser Val Asn Asn Ile Asn Ser Val Asn 705 710 715 720 Asn Ile Asn Ser Val Asn Asn Ile Asn Ser Val Asn Asn Ile Asn Ser 725 730 735 Val Asn Asn Ile Asn Ser Val Asn Asn Leu Asn Ser Val Asn Asn Ile 740 745 750 Asn Ser Val Asn Asn Ile Asn Asn Ile Asn Tyr Ile Asn Asn Ile Asn 755 760 765 Tyr Val Asn Met Asn Lys Gly Leu Asn Pro Ile Asn Asn Val Ser Asn 770 775 780 Ile Ser Ser Leu Lys Leu Leu Asn Asn Asn Asn Asp Ile Lys Lys Lys 785 790 795 800 Phe Asn Thr Tyr Gly Lys Ser Glu Ala Ser Glu Asn Leu Ser Lys Asn 805 810 815 Val Lys Tyr Ile Lys Tyr Ile Gln Glu Asn Ile Lys Tyr Leu Asn Asn 820 825 830 Leu Asp Asp Asn Lys Arg Lys Tyr Ser Leu Thr Ser Ile Asn Asp Val 835 840 845 Gly Cys Ile Lys Lys Lys Lys Asn Met Asn Asp Leu Phe Leu Gly Lys 850 855 860 His Asp Asn Met Leu Arg Thr Asp Glu Ile Pro Lys Ile Asn Leu Gly 865 870 875 880 Lys Asn Ile Leu Asn Asn Asn Lys Ile Ile Asn Tyr Asn Asp Asn Asp 885 890 895 Lys Ser Asn Ile Ile Asn Asn Val Ile Asn Lys Asn Ile Ser Thr Asp 900 905 910 Leu Val Asn Asp Arg Glu Gly Asp Met Asn Lys Met Asn Ile His Asn 915 920 925 Arg Glu Lys Asp Glu Asn Asn Tyr Ile Asn Ile Gly Asp Asn Lys Ile 930 935 940 Lys Lys Asn Gln Ile Asp Val Val Asn Asn Lys Val Met Lys Leu Asp 945 950 955 960 Asn Met Glu Asp Glu Glu Ala Met Asn Lys Leu Ser Leu Ile Ser Leu 965 970 975 Tyr Pro Asn Asn Asn His Ile Ile Asn Asn Val Asn Asn Val Asn Asn 980 985 990 Val Asn Asn Val Asn Asn Val Asn Asn Val Asn Asn Val Asn Asn Val 995 1000 1005 Asn Asn Val Asn Asn Val Asn Asn Val Asn Tyr Met Asn Asn Met 1010 1015 1020 Asn Asn Val Asn Asn Val Asn Asn Met Asn Asn Val Asn Asn Met 1025 1030 1035 Asn Asn Val Asn Asn Val Asn Ser Val Asn Asn Ile Lys Gly Ile 1040 1045 1050 Asn Asn Met Asn Asn Asn Asn Asn Asn Ile Asn Met Asn Arg Ser 1055 1060 1065 Tyr Lys Met Asn Met Lys Lys Val Ser Lys Lys Asp Asn Gly Gln 1070 1075 1080 Asn Val Val Ser Glu Lys Arg Phe Ser Glu Glu Lys Tyr Asn Phe 1085 1090 1095 Leu Lys Asn Leu Ile Arg Asn Asn Lys Asn Met Val Lys Leu Lys 1100 1105 1110 Tyr Leu Asn Lys Phe Leu Gly Lys Arg Ser Gly Pro Ser Ile Lys 1115 1120 1125 Asn Asn Met Asn Asp Met Met Val Lys Met Asn Asn Asn Met Lys 1130 1135 1140 Asp Ile Met His Ile Lys Asp Ala Thr Asn Ile Asn Lys Ile Asn 1145 1150 1155 Asn Lys Leu Val Asn Leu Asn Thr Asn Asn Cys Ile Ser Tyr Asn 1160 1165 1170 Ser Cys Asn Lys Met Asn Tyr Ile His Lys Cys Lys Lys Lys Arg 1175 1180 1185 Val Leu Cys Leu Asp Thr Lys His Gly Lys Asn Glu Ile Lys Gln 1190 1195 1200 Asn Glu Lys Leu Ile Tyr Thr Asn Tyr Glu Ile Lys Met Phe Leu 1205 1210 1215 Leu Asn Thr Ile Lys Ala Ile Gly Ile Val Phe Lys Lys Trp Lys 1220 1225 1230 Phe Lys Asn Phe Gly Leu Tyr Phe Trp Tyr His Ile Lys Cys Ile 1235 1240 1245 Glu Asn Glu Arg Asp Leu Asn Phe Tyr Ile Lys Ile Phe Asn Phe 1250 1255 1260 Leu Phe Glu Ile Ile Thr Gly Lys Asn Ile Tyr Tyr Gln Ile Asn 1265 1270 1275 Asp Ile His Asn Ile Val Ala Leu Phe Lys Glu Phe Lys Ile Tyr 1280 1285 1290 Asp Cys Lys His Val Leu Lys Lys Ser Ile Lys Val Leu Asn Lys 1295 1300 1305 Tyr Ala Lys Lys Asn Ser Lys Glu Phe Ser Leu Phe Glu Asn Asn 1310 1315 1320 Gln His Val Val Leu Asp Ile Asn Lys His Met Leu Phe Asn Asp 1325 1330 1335 Asp Glu Lys Lys Leu Thr Thr Cys Asn Ile Lys Gln Asn Glu Gln 1340 1345 1350 Glu Gln Ile Lys Thr Lys Val Leu Tyr Asp His Asp Asn Ile Asn 1355 1360 1365 Val Asp Thr Lys Gln Asn Tyr Gln Lys Ile Ile Thr Asn Lys Asn 1370 1375 1380 Asn His Pro Lys Asp Asn Phe Tyr Ser Tyr Leu Tyr Asp Ser Leu 1385 1390 1395 Gln Gly Lys Asn His Ile Phe Gln Gln Pro Gly Val Gln Asn Met 1400 1405 1410 His Ile Tyr Asn Met Phe Ala Gln Phe Asn Glu Leu Asn Phe Asn 1415 1420 1425 Asp Met Phe Asn Phe Ser Ile Thr 1430 1435 54548DNAPlasmodium falciparum 5atgtttttta tgtggttata taaatataag gtgtacatat atgtcatatt ttttgtactt 60gttgaaaata cgatgagtat gaaaaagaat ttgatgttca catgttttaa taaaaaaaag 120tttatatatg taaagaagaa tgtattattt ttgaataata aatatataag taatttggta 180tataccaagg aagatgatat atataaaatt aatgagataa gtaatgttat aggaagaaat 240agaaagaaaa agaatataat aataaataat gatgaggata atgataatat tgatagtaag 300aataatgaac tgggtgataa tataatatca tcgaatagat atatgaataa tgtatttttt 360acaaataata aaaatacatc actggaagtt agaaataata ataatgaaga tggtggtgat 420gataacaaca acaataataa taataataat aataatatat tagatgtgtc atataatcat 480gataaatata atttgtgtga taacgaatta caattatata aaaataaatg taaaattcca 540ctttattgga ttaaaaaatt agataactta aaaaatatga atgctataaa ttgtatagaa 600tatttgaaag atgataattt gttatatttt gataattata agaacaaagg attattaaaa 660tttctaaatg atgaaaagag gaaatataat aattgtataa ttttgtcaag ggtaggtgat 720ttttatgaaa catatggcct ggattctata ttcctcattg aatttttaaa cataaaaaaa 780atgaataata gattatcatg tggttttatc aaaagcagta taaataaagc tttaaatata 840ttaactaaca ataatttaaa tgtatgtata tatgaagaaa taaatgaaaa gtctttaaaa 900atgaaaaaga gatatttatc gcaaattgta acacctgaat ttcctatata tttaaataat 960atacaatact ataataagga tgaagatata ctacaaaatg gtaacaacaa taataataat 1020agtaataata tgaatagtta taataacatg gataaatgtt tagataatgg aaatatgaat 1080gcactatcct tttttagttc ttatgatcta gatgattatt ttgttataaa agaaattgtt 1140tgtatatata tagaaagcaa aaatattttt tcattaagta aaattaattt aagcttaaaa 1200actatatcta tatatgataa tataacattt gatgttttaa atgtatattt gaaaaatacg 1260aattttttga aggtatatat acatcaacat attaatacat catttaccaa aaaaatcaca 1320gagttattca aaatagaaaa ttattatttg tttaataatt tcaagtcaag tttttatttt 1380catatgttta tattagacaa gctaaaaaaa caaatacacg taaaggggtt attcagattg 1440ataaagaata aaaatgtttt caaaattaaa tcagctgatt gtaagaagga agagaaaaaa 1500attgattcga atattagtga ttatatgaat tatgaggaga ataataaaat tagcaataga 1560catataaata atgtaaataa taatattgaa gataatcaaa atgtagaaat aaataaaaat 1620gtggaagata ataatattga gaaaattaaa aataaaaaca ttatagataa taattttgaa 1680tattcttttt catcttattg tacaccttta aatatattta cgagttataa tatgggtata 1740tataaacaaa ataatcatta tgaaaataaa agtaattttt tattttataa tattatagat 1800ataaataata ataattcaaa tagtataaat atatcagaat cattagagtt ttttaaaaat 1860atttttcttt tttatccacc ttttgaagtt acaaaacata taagatatat aaatgaatat 1920attaaaaaga atgtgaaaga tttaatcata ccgaatgtaa aaccatttaa gaataatatt 1980attatatctc ttttatcaaa tttaaaagcc gatcatcaca ttttaaaaaa aatattagta 2040aatatagatg ctgtactaaa ttgtattaga aattatgatt tctcactttt agtatcaata 2100tttaatgtat taaatcatca aaattctttt aaattaaata ttataaaatt ttatgaactt 2160ttaatgaata ttcaaaaaat tatgaaagac aatttggatt taggtttatt ttctaaattc 2220tcttatcagt cagatattca agcttttaat gattttgtta tatatcatga aaacgatgta 2280cacaatataa ttaatgaaaa acttataact gaagaaaata aagaattaga aaaatccaga 2340acagatttat tgaatacaat tatatcaaat tattcagaat gtgataaaac aaaaaatgca 2400tataaagata ttaatttatt aaacaaaata ataaaaattg ataatgctaa tgatataata 2460ggtatacgta aaaatataca aaaaaaaaaa aacaaaaatg aacaaatttc agattttttt 2520catccattaa acaaaaaatc agatgttatg aaaaatttat atgtaactca agatgtacaa 2580aataagatta aaacttattt attctcaatt tataaaaaaa aaaaaaaaat aaatgaaatt 2640attcacaata taaatataca attgtcttcc tcagtgcaca tactttcatt tgtatctaac 2700ttcttgcaga taattcaggc tctatacaac cataccatta acagtctcaa gaaaggatgg 2760tccctaccaa tatgtaaaca cctacatttg tcatatgaaa atatgagctt caacaatatc 2820gacgataagt tagcttatat acaaaaaaaa gtgtacgatg acaaagacga aataaaagag 2880tacacattaa caaagaatgt aaataaaaat gatccttcga attattttat aaatgatcaa 2940aagaatagca atgaatttgt aaaattatct ccatgtgatg aaaagaaaat tttagaaaac 3000atagacaaag attcagcaga agaaataaaa aaaatgtata aagaaaaaaa ttacgttaac 3060gattcaataa catatataat aggagccaaa ccttataata taataaaaca taatttaatt 3120aaatatgatt tttttttaaa aaagaaaaat tttattttat tgactggaaa aaatatgagt 3180ggaaaaacta cattgtcttt tacaattttg agtatattat ttttgtccaa tttgggtatg 3240tatgcaccgt gtgatgaaaa cagcattata ggaaagtttc gagaatttta tagtttgaaa 3300aatgtaaact atcaagagca aattgaaaat atgtccttat ttagggaaca agcttattat 3360atcaattcag ttatagaaga aataaaagaa aattattctg ttgataaaag accaacaaga 3420gaaaaggaaa tttttattgt cctcgatgaa ccatgtatag caaccacacc tgttgataat 3480gcaattatta taagcgcagt ttcagattac ctgaaaaatt attgtggaat tataattaca 3540cataactacg atttattaaa caaaatatgc cagagcgaaa acatcgtttt taaaagaatt 3600agtaaagata ttaactacct gaaagaacaa gataataaaa tagttggtaa attagaagac 3660ggtatttgta aaaacagtga agccttagaa acatgtagat atacaaatat agacccacat 3720gttttaaatt tattaaatgt atatgaaaaa aaatataaat ttattcataa tttaagtaat 3780acactatata ccaaattttt agaatatatt caatatagaa aagatgggaa gtgtctcaat 3840gacttttttg atcaatatat aaatgatata ataaaacaaa agagtgataa acatgaaata 3900caatataata cacctagtaa tatggaaaat aattataata gttatagttc aaatgatcaa 3960aagaattata tatcagaaaa tcacaagtat gattataatt atgatatcga ttacataaaa 4020gataaagaat taaaaaatat aataaattct aataataata tacaagaatt tttaaacaat 4080tatgataaga caaggataca taatgataat ccaactaatc ataaaccaga tcaatatgat 4140aactcattag tttatgatga taaactaaat gttataatta aaaaaattga agaagcatca 4200aataagaagg ttataaaaat aggaatgaat gaagaagtac ctatttatta taaaaataaa 4260agtattgtat atattttatg tattttttca aaagatgaaa atgatccata tttttatata 4320ggtataagtg ataatatttc agaaaggata aaatgtcata caagaaatct attaaataat 4380aaaaatcttt taaaaaatcc aaagaaaaat aatctattaa attataaata tgattggact 4440aaattctata ttttactttt tcatgttgat aataaaatgg ttgcatccaa atatgaaaag 4500gacctttcga atttgttgaa gaataattat aacatattat ctaaataa 454861515PRTPlasmodium falciparum 6Met Phe Phe Met Trp Leu Tyr Lys Tyr Lys Val Tyr Ile Tyr Val Ile 1 5 10 15 Phe Phe Val Leu Val Glu Asn Thr Met Ser Met Lys Lys Asn Leu Met 20 25 30 Phe Thr Cys Phe Asn Lys Lys Lys Phe Ile Tyr Val Lys Lys Asn Val 35 40 45 Leu Phe Leu Asn Asn Lys Tyr Ile Ser Asn Leu Val Tyr Thr Lys Glu 50 55 60 Asp Asp Ile Tyr Lys Ile Asn Glu Ile Ser Asn Val Ile Gly Arg Asn 65 70 75 80 Arg Lys Lys Lys Asn Ile Ile Ile Asn Asn Asp Glu Asp Asn Asp Asn 85 90 95 Ile Asp Ser Lys Asn Asn Glu Leu Gly Asp Asn Ile Ile Ser Ser Asn 100 105 110 Arg Tyr Met Asn Asn Val Phe Phe Thr Asn Asn Lys Asn Thr Ser Leu 115 120 125 Glu Val Arg Asn Asn Asn Asn Glu Asp Gly Gly Asp Asp Asn Asn Asn 130 135 140 Asn Asn Asn Asn Asn Asn Asn Asn Ile Leu Asp Val Ser Tyr Asn His 145 150 155 160 Asp Lys Tyr Asn Leu Cys Asp Asn Glu Leu Gln Leu Tyr Lys Asn Lys 165 170 175 Cys Lys Ile Pro Leu Tyr Trp Ile Lys Lys Leu Asp Asn Leu Lys Asn 180 185 190 Met Asn Ala Ile Asn Cys Ile Glu Tyr Leu Lys Asp Asp Asn Leu Leu 195 200

205 Tyr Phe Asp Asn Tyr Lys Asn Lys Gly Leu Leu Lys Phe Leu Asn Asp 210 215 220 Glu Lys Arg Lys Tyr Asn Asn Cys Ile Ile Leu Ser Arg Val Gly Asp 225 230 235 240 Phe Tyr Glu Thr Tyr Gly Leu Asp Ser Ile Phe Leu Ile Glu Phe Leu 245 250 255 Asn Ile Lys Lys Met Asn Asn Arg Leu Ser Cys Gly Phe Ile Lys Ser 260 265 270 Ser Ile Asn Lys Ala Leu Asn Ile Leu Thr Asn Asn Asn Leu Asn Val 275 280 285 Cys Ile Tyr Glu Glu Ile Asn Glu Lys Ser Leu Lys Met Lys Lys Arg 290 295 300 Tyr Leu Ser Gln Ile Val Thr Pro Glu Phe Pro Ile Tyr Leu Asn Asn 305 310 315 320 Ile Gln Tyr Tyr Asn Lys Asp Glu Asp Ile Leu Gln Asn Gly Asn Asn 325 330 335 Asn Asn Asn Asn Ser Asn Asn Met Asn Ser Tyr Asn Asn Met Asp Lys 340 345 350 Cys Leu Asp Asn Gly Asn Met Asn Ala Leu Ser Phe Phe Ser Ser Tyr 355 360 365 Asp Leu Asp Asp Tyr Phe Val Ile Lys Glu Ile Val Cys Ile Tyr Ile 370 375 380 Glu Ser Lys Asn Ile Phe Ser Leu Ser Lys Ile Asn Leu Ser Leu Lys 385 390 395 400 Thr Ile Ser Ile Tyr Asp Asn Ile Thr Phe Asp Val Leu Asn Val Tyr 405 410 415 Leu Lys Asn Thr Asn Phe Leu Lys Val Tyr Ile His Gln His Ile Asn 420 425 430 Thr Ser Phe Thr Lys Lys Ile Thr Glu Leu Phe Lys Ile Glu Asn Tyr 435 440 445 Tyr Leu Phe Asn Asn Phe Lys Ser Ser Phe Tyr Phe His Met Phe Ile 450 455 460 Leu Asp Lys Leu Lys Lys Gln Ile His Val Lys Gly Leu Phe Arg Leu 465 470 475 480 Ile Lys Asn Lys Asn Val Phe Lys Ile Lys Ser Ala Asp Cys Lys Lys 485 490 495 Glu Glu Lys Lys Ile Asp Ser Asn Ile Ser Asp Tyr Met Asn Tyr Glu 500 505 510 Glu Asn Asn Lys Ile Ser Asn Arg His Ile Asn Asn Val Asn Asn Asn 515 520 525 Ile Glu Asp Asn Gln Asn Val Glu Ile Asn Lys Asn Val Glu Asp Asn 530 535 540 Asn Ile Glu Lys Ile Lys Asn Lys Asn Ile Ile Asp Asn Asn Phe Glu 545 550 555 560 Tyr Ser Phe Ser Ser Tyr Cys Thr Pro Leu Asn Ile Phe Thr Ser Tyr 565 570 575 Asn Met Gly Ile Tyr Lys Gln Asn Asn His Tyr Glu Asn Lys Ser Asn 580 585 590 Phe Leu Phe Tyr Asn Ile Ile Asp Ile Asn Asn Asn Asn Ser Asn Ser 595 600 605 Ile Asn Ile Ser Glu Ser Leu Glu Phe Phe Lys Asn Ile Phe Leu Phe 610 615 620 Tyr Pro Pro Phe Glu Val Thr Lys His Ile Arg Tyr Ile Asn Glu Tyr 625 630 635 640 Ile Lys Lys Asn Val Lys Asp Leu Ile Ile Pro Asn Val Lys Pro Phe 645 650 655 Lys Asn Asn Ile Ile Ile Ser Leu Leu Ser Asn Leu Lys Ala Asp His 660 665 670 His Ile Leu Lys Lys Ile Leu Val Asn Ile Asp Ala Val Leu Asn Cys 675 680 685 Ile Arg Asn Tyr Asp Phe Ser Leu Leu Val Ser Ile Phe Asn Val Leu 690 695 700 Asn His Gln Asn Ser Phe Lys Leu Asn Ile Ile Lys Phe Tyr Glu Leu 705 710 715 720 Leu Met Asn Ile Gln Lys Ile Met Lys Asp Asn Leu Asp Leu Gly Leu 725 730 735 Phe Ser Lys Phe Ser Tyr Gln Ser Asp Ile Gln Ala Phe Asn Asp Phe 740 745 750 Val Ile Tyr His Glu Asn Asp Val His Asn Ile Ile Asn Glu Lys Leu 755 760 765 Ile Thr Glu Glu Asn Lys Glu Leu Glu Lys Ser Arg Thr Asp Leu Leu 770 775 780 Asn Thr Ile Ile Ser Asn Tyr Ser Glu Cys Asp Lys Thr Lys Asn Ala 785 790 795 800 Tyr Lys Asp Ile Asn Leu Leu Asn Lys Ile Ile Lys Ile Asp Asn Ala 805 810 815 Asn Asp Ile Ile Gly Ile Arg Lys Asn Ile Gln Lys Lys Lys Asn Lys 820 825 830 Asn Glu Gln Ile Ser Asp Phe Phe His Pro Leu Asn Lys Lys Ser Asp 835 840 845 Val Met Lys Asn Leu Tyr Val Thr Gln Asp Val Gln Asn Lys Ile Lys 850 855 860 Thr Tyr Leu Phe Ser Ile Tyr Lys Lys Lys Lys Lys Ile Asn Glu Ile 865 870 875 880 Ile His Asn Ile Asn Ile Gln Leu Ser Ser Ser Val His Ile Leu Ser 885 890 895 Phe Val Ser Asn Phe Leu Gln Ile Ile Gln Ala Leu Tyr Asn His Thr 900 905 910 Ile Asn Ser Leu Lys Lys Gly Trp Ser Leu Pro Ile Cys Lys His Leu 915 920 925 His Leu Ser Tyr Glu Asn Met Ser Phe Asn Asn Ile Asp Asp Lys Leu 930 935 940 Ala Tyr Ile Gln Lys Lys Val Tyr Asp Asp Lys Asp Glu Ile Lys Glu 945 950 955 960 Tyr Thr Leu Thr Lys Asn Val Asn Lys Asn Asp Pro Ser Asn Tyr Phe 965 970 975 Ile Asn Asp Gln Lys Asn Ser Asn Glu Phe Val Lys Leu Ser Pro Cys 980 985 990 Asp Glu Lys Lys Ile Leu Glu Asn Ile Asp Lys Asp Ser Ala Glu Glu 995 1000 1005 Ile Lys Lys Met Tyr Lys Glu Lys Asn Tyr Val Asn Asp Ser Ile 1010 1015 1020 Thr Tyr Ile Ile Gly Ala Lys Pro Tyr Asn Ile Ile Lys His Asn 1025 1030 1035 Leu Ile Lys Tyr Asp Phe Phe Leu Lys Lys Lys Asn Phe Ile Leu 1040 1045 1050 Leu Thr Gly Lys Asn Met Ser Gly Lys Thr Thr Leu Ser Phe Thr 1055 1060 1065 Ile Leu Ser Ile Leu Phe Leu Ser Asn Leu Gly Met Tyr Ala Pro 1070 1075 1080 Cys Asp Glu Asn Ser Ile Ile Gly Lys Phe Arg Glu Phe Tyr Ser 1085 1090 1095 Leu Lys Asn Val Asn Tyr Gln Glu Gln Ile Glu Asn Met Ser Leu 1100 1105 1110 Phe Arg Glu Gln Ala Tyr Tyr Ile Asn Ser Val Ile Glu Glu Ile 1115 1120 1125 Lys Glu Asn Tyr Ser Val Asp Lys Arg Pro Thr Arg Glu Lys Glu 1130 1135 1140 Ile Phe Ile Val Leu Asp Glu Pro Cys Ile Ala Thr Thr Pro Val 1145 1150 1155 Asp Asn Ala Ile Ile Ile Ser Ala Val Ser Asp Tyr Leu Lys Asn 1160 1165 1170 Tyr Cys Gly Ile Ile Ile Thr His Asn Tyr Asp Leu Leu Asn Lys 1175 1180 1185 Ile Cys Gln Ser Glu Asn Ile Val Phe Lys Arg Ile Ser Lys Asp 1190 1195 1200 Ile Asn Tyr Leu Lys Glu Gln Asp Asn Lys Ile Val Gly Lys Leu 1205 1210 1215 Glu Asp Gly Ile Cys Lys Asn Ser Glu Ala Leu Glu Thr Cys Arg 1220 1225 1230 Tyr Thr Asn Ile Asp Pro His Val Leu Asn Leu Leu Asn Val Tyr 1235 1240 1245 Glu Lys Lys Tyr Lys Phe Ile His Asn Leu Ser Asn Thr Leu Tyr 1250 1255 1260 Thr Lys Phe Leu Glu Tyr Ile Gln Tyr Arg Lys Asp Gly Lys Cys 1265 1270 1275 Leu Asn Asp Phe Phe Asp Gln Tyr Ile Asn Asp Ile Ile Lys Gln 1280 1285 1290 Lys Ser Asp Lys His Glu Ile Gln Tyr Asn Thr Pro Ser Asn Met 1295 1300 1305 Glu Asn Asn Tyr Asn Ser Tyr Ser Ser Asn Asp Gln Lys Asn Tyr 1310 1315 1320 Ile Ser Glu Asn His Lys Tyr Asp Tyr Asn Tyr Asp Ile Asp Tyr 1325 1330 1335 Ile Lys Asp Lys Glu Leu Lys Asn Ile Ile Asn Ser Asn Asn Asn 1340 1345 1350 Ile Gln Glu Phe Leu Asn Asn Tyr Asp Lys Thr Arg Ile His Asn 1355 1360 1365 Asp Asn Pro Thr Asn His Lys Pro Asp Gln Tyr Asp Asn Ser Leu 1370 1375 1380 Val Tyr Asp Asp Lys Leu Asn Val Ile Ile Lys Lys Ile Glu Glu 1385 1390 1395 Ala Ser Asn Lys Lys Val Ile Lys Ile Gly Met Asn Glu Glu Val 1400 1405 1410 Pro Ile Tyr Tyr Lys Asn Lys Ser Ile Val Tyr Ile Leu Cys Ile 1415 1420 1425 Phe Ser Lys Asp Glu Asn Asp Pro Tyr Phe Tyr Ile Gly Ile Ser 1430 1435 1440 Asp Asn Ile Ser Glu Arg Ile Lys Cys His Thr Arg Asn Leu Leu 1445 1450 1455 Asn Asn Lys Asn Leu Leu Lys Asn Pro Lys Lys Asn Asn Leu Leu 1460 1465 1470 Asn Tyr Lys Tyr Asp Trp Thr Lys Phe Tyr Ile Leu Leu Phe His 1475 1480 1485 Val Asp Asn Lys Met Val Ala Ser Lys Tyr Glu Lys Asp Leu Ser 1490 1495 1500 Asn Leu Leu Lys Asn Asn Tyr Asn Ile Leu Ser Lys 1505 1510 1515 71728DNAPlasmodium falciparum 7atgagcgaag atataaaatt tgtagaacgc aacaacaata ataatattaa taacagcata 60ccgaacaata ttataaatag tgtatccatt gcgacggata attcaaagtt gaacatatcg 120ccaaaggttg ataatgtatg tgatgtgaaa aatatttata atataaaaaa agaagatgtt 180ataattgaag agtatatgga tattaataaa agtagtacaa taaatctaga tcataataaa 240caagttaata tatatattga aaatagcgat cttaataaaa aaatatatac atgtcataca 300tgtaatatac aaatatataa ttattccttc tttcgttatc attttaaatc cgaatggcat 360aaatataatt tgaaaagaaa attattaaat ctacattcag taaatgaata tgtttttaat 420gaaaaattaa aaaatttaaa aaaaaatgaa gatcaagaaa atcaaaagaa agacaaaaga 480gaaaatcaag ggagccattt aagtcataaa aaacgtaacg aaagtaataa aaaaaaaaag 540gaaaaaaata tcacgaattt taataatatt aataatgatc ataatgcaat caatcaaaat 600aattataata ataataatca tgtgaccaaa tcgaataatc ataccaatga acataacaat 660cattcaaatg tttcatccac atcaaataat aacaatataa aatacgctac aaaagaaaat 720gtacttttaa caaaaaatgc taaatatgac aatccagctg tctgtttttt tgataatcgt 780atttttaatt ccatagaaga aaatataaaa catatgaatg atacttatac attttatatt 840cctgatttaa aatacgtaac caatgtgaaa aaaatcctat taacaattgg aaagaaaata 900tatgaagaaa atatatgtat ctattgtttt aaatatgcga aatgtgttaa atctttacaa 960gcacatatga tttgtaaaag ccatacgaag ttacatacca attttatggt ctatatccaa 1020aaatattatg acttctcaaa aacttatgtt gatttattaa ataaatatat taataataaa 1080caagataaaa aattactcct ctatatgtta aatcacgaac aaaataaaga gaaacaatta 1140caaattcatg ataataaaaa tcaaaatcat cataatgaca accatttgga aaataacaat 1200gttcttacga aaaagaaact ttctaatgat acagacaata attctgagga ctatcctaat 1260gatagttata tattaaaaaa agaaaagaac caagatacat ctttatcaaa ctcttatgat 1320aatcatgata ataccagtga tgataatagt agcgacgatt ataaaataaa agaaacagat 1380ctaaataaaa atattgacta taataaaatt tatcaagtat tagaagaatt tggatacatc 1440aaacctgaat taaatgaata taacaattta attctaccag atggttcaga agccatcaat 1500agaaaacttg cttacatatt taaacaaaaa ctacccttgg aaaataaaac aagcttttct 1560tccaaatata atcaaatcga tgttttaaaa aataaaaaag acaaacactt acaacaacgc 1620aaatataaat attatattga tattatgaag aaatataatt taaatctaaa tttgaagaca 1680aacaatttga acaagtttta caaaagtgat tctatcttct tcctctaa 17288575PRTPlasmodium falciparum 8Met Ser Glu Asp Ile Lys Phe Val Glu Arg Asn Asn Asn Asn Asn Ile 1 5 10 15 Asn Asn Ser Ile Pro Asn Asn Ile Ile Asn Ser Val Ser Ile Ala Thr 20 25 30 Asp Asn Ser Lys Leu Asn Ile Ser Pro Lys Val Asp Asn Val Cys Asp 35 40 45 Val Lys Asn Ile Tyr Asn Ile Lys Lys Glu Asp Val Ile Ile Glu Glu 50 55 60 Tyr Met Asp Ile Asn Lys Ser Ser Thr Ile Asn Leu Asp His Asn Lys 65 70 75 80 Gln Val Asn Ile Tyr Ile Glu Asn Ser Asp Leu Asn Lys Lys Ile Tyr 85 90 95 Thr Cys His Thr Cys Asn Ile Gln Ile Tyr Asn Tyr Ser Phe Phe Arg 100 105 110 Tyr His Phe Lys Ser Glu Trp His Lys Tyr Asn Leu Lys Arg Lys Leu 115 120 125 Leu Asn Leu His Ser Val Asn Glu Tyr Val Phe Asn Glu Lys Leu Lys 130 135 140 Asn Leu Lys Lys Asn Glu Asp Gln Glu Asn Gln Lys Lys Asp Lys Arg 145 150 155 160 Glu Asn Gln Gly Ser His Leu Ser His Lys Lys Arg Asn Glu Ser Asn 165 170 175 Lys Lys Lys Lys Glu Lys Asn Ile Thr Asn Phe Asn Asn Ile Asn Asn 180 185 190 Asp His Asn Ala Ile Asn Gln Asn Asn Tyr Asn Asn Asn Asn His Val 195 200 205 Thr Lys Ser Asn Asn His Thr Asn Glu His Asn Asn His Ser Asn Val 210 215 220 Ser Ser Thr Ser Asn Asn Asn Asn Ile Lys Tyr Ala Thr Lys Glu Asn 225 230 235 240 Val Leu Leu Thr Lys Asn Ala Lys Tyr Asp Asn Pro Ala Val Cys Phe 245 250 255 Phe Asp Asn Arg Ile Phe Asn Ser Ile Glu Glu Asn Ile Lys His Met 260 265 270 Asn Asp Thr Tyr Thr Phe Tyr Ile Pro Asp Leu Lys Tyr Val Thr Asn 275 280 285 Val Lys Lys Ile Leu Leu Thr Ile Gly Lys Lys Ile Tyr Glu Glu Asn 290 295 300 Ile Cys Ile Tyr Cys Phe Lys Tyr Ala Lys Cys Val Lys Ser Leu Gln 305 310 315 320 Ala His Met Ile Cys Lys Ser His Thr Lys Leu His Thr Asn Phe Met 325 330 335 Val Tyr Ile Gln Lys Tyr Tyr Asp Phe Ser Lys Thr Tyr Val Asp Leu 340 345 350 Leu Asn Lys Tyr Ile Asn Asn Lys Gln Asp Lys Lys Leu Leu Leu Tyr 355 360 365 Met Leu Asn His Glu Gln Asn Lys Glu Lys Gln Leu Gln Ile His Asp 370 375 380 Asn Lys Asn Gln Asn His His Asn Asp Asn His Leu Glu Asn Asn Asn 385 390 395 400 Val Leu Thr Lys Lys Lys Leu Ser Asn Asp Thr Asp Asn Asn Ser Glu 405 410 415 Asp Tyr Pro Asn Asp Ser Tyr Ile Leu Lys Lys Glu Lys Asn Gln Asp 420 425 430 Thr Ser Leu Ser Asn Ser Tyr Asp Asn His Asp Asn Thr Ser Asp Asp 435 440 445 Asn Ser Ser Asp Asp Tyr Lys Ile Lys Glu Thr Asp Leu Asn Lys Asn 450 455 460 Ile Asp Tyr Asn Lys Ile Tyr Gln Val Leu Glu Glu Phe Gly Tyr Ile 465 470 475 480 Lys Pro Glu Leu Asn Glu Tyr Asn Asn Leu Ile Leu Pro Asp Gly Ser 485 490 495 Glu Ala Ile Asn Arg Lys Leu Ala Tyr Ile Phe Lys Gln Lys Leu Pro 500 505 510 Leu Glu Asn Lys Thr Ser Phe Ser Ser Lys Tyr Asn Gln Ile Asp Val 515 520 525 Leu Lys Asn Lys Lys Asp Lys His Leu Gln Gln Arg Lys Tyr Lys Tyr 530 535 540 Tyr Ile Asp Ile Met Lys Lys Tyr Asn Leu Asn Leu Asn Leu Lys Thr 545 550 555 560 Asn Asn Leu Asn Lys Phe Tyr Lys Ser Asp Ser Ile Phe Phe Leu 565 570 575 916320DNAPlasmodium falciparum 9 atggaaccta aagaaactga taatgtaata aaagatgagg acaatgtgaa aaaggaagaa 60cctatattat ctattgaaaa atatttatta aaagagtttg gtgatgggaa aacgaataag 120aaggatgtta tgagaaaatt gagaaagagg atatataatc ataaaaattc agaagagata 180tacaaaaaat taaaagagat tcatgggaat agtggtatat gtacacgtca atggaaggaa 240ggtagttttg cttttaagtg ttataattgt gaaggggatc caacgtgtgc tatatgtgca 300aaatgttttt ttgcatcaaa tcataagaat catatatata ggttaacaca tacaagtggt 360ggttgttgtg attgtggaga tacttcatgg aatattaatg gttcatgttt tgatcataga 420ggaattaatg aagattgttt gattaacaat tctatattaa atattgatat aaagaatcgt 480gtaagagaag atttggaatg tttgatagga acattattta gggatatagt attaaaagat 540ggatattttt tatctttagt aagtgctgat tatatagagc atgcatttga tttttttaaa 600gatttaggta ttacaagtta tttatttcgg caagttatct gtgaagtatt tacaggtgca 660aaaatagatt ttttagtaaa ttttcattat tgtttttatg taggtgtaca gaaagcttta 720tattcttttt atttatcttt atttgttcat

ccatatttta aacaaaggtt tgcttttaag 780ttagctaaac attataaatt ggttgttcgt gttgctgatg atcgaattta taatgataat 840catttgaatg gtttaagtgt tcaggtatta actgttcccg aaatagcata tactttagtt 900ataaataatt tcttgaatga tttattaaaa ttaatagaaa atttatgtgt atttaataat 960gtaacaaatt gtgttatgca taaaaatttc ttacgatctg atatcgaaat tataattcgt 1020atatgtgttg atttgaaata tttgctttat catcatgaag ttataaaaat tgtattattt 1080aataaatatg tcattaaaaa aatattaaaa atattatctc ttttgcatag aatgaattca 1140caagtacgat atacaaaatc gcatattata tatgaagatt tatctagttc ctattccata 1200acgattgaaa attatttact tcgtgtattt gaacccttgg ctaatttttg taaaagagaa 1260gctaatgcaa attttagaaa tttattttta ttgtatcaat taacaattag aggtatacat 1320aaattggatt tgtttccatt taatcgaaaa aaaaaggagg cagaaaattc ttttatgaat 1380cgcaaaaaat tgaaggaaaa aaaaattaca aaaaagagga atattaaaga ggataattca 1440acggatgact tgacaggtga tagtgataat agtagtacta ataatgtttg tcgctgtgat 1500actagtaatt atgataatac gatgtttgat cgtagttgta gtataccagg gttaaataat 1560atgaatgatg acgttaataa atctggaggg gtatctttaa atgatgataa tataaataat 1620gataataata atacaaataa taatacaaac aataatacaa acaataataa taataataca 1680aataataata caaacaataa taataataat aaaaattgtg agggcaaggc caatgatcaa 1740acgaataaga aaaaaaaatt caaacatctt agatctttac attctccatt agttcgattc 1800tttatcatga ttttaaattt tgatgtcatt aaaaaatgcc tagatgacgt gtactggtat 1860aaatatttat atttaagaag ggtttatgaa aaaaaaagga tatataaaaa tagaaaaaaa 1920agtcctgcat ataataccaa tgatgataat aaaaatgaag tgtgtactaa aaatattcca 1980tgtgatgata ataaaaatga aatgtgtact aaaaatattc catgtgatga taataaaaat 2040gaagtgtgta ctaaaaatat tccatgtgat gataataaaa atgaagtgtg tactaaaaat 2100attccatgtg atgataataa aaatgaaatg tgtactaaaa atattccatg tgatgataat 2160aaaaatattc caagagatga taataaatca tataataaaa tgtacaataa taataaaatg 2220aataaggagt taacatataa tgatgaatat ataataaatg aatttattag agatttttat 2280tctatagaaa agcattcaga agaaataata aatcagcgaa aaatattaca agaaagatat 2340gaaaacgatg gtaataatat gaagataaca ctttttcatt ttaaaaaatt ttttacaagt 2400aatgatgaac ataagaatga acatttagga aaatatatag atgaacatat atatttagaa 2460agtaattatt ttaaatggat tatgaataat aattctagtt ctaaattacg tgaatgtaat 2520aataagtata gaattaaata tagagaaacc atacaagaaa agaatataaa aataaaatgt 2580aaaaatatat atatagagaa aaaattaaaa aattcaaaat atgaatttaa tgaatttttg 2640gatataaaat atgataaaga aaaacataat atatatttac atcctttata tatagattta 2700tatatgagag atatatttga gtttttaaat ttatttgatt taaaattatt aaaaaatatt 2760ctttttataa atatacaaat cttaacattt atatatgaaa ttaaatatgg ttattgggta 2820agaaatggtc cacaaatgat tttccaggtt ggtgaatatg ataattcttt attttttcaa 2880aatgatttaa taggaataca atttgctata ataatgatga atgtcttacc tttatacgaa 2940aatcaaaaag tttatttaga ttcacatgtg atcaatctat ttcatcaaat atggaatatt 3000aaggttcaat acgatgaacc acttaaaaaa ttaccatggt caaaaaatga atacaacgat 3060gtgcaaaatg attatataca taaagctgat gatgaaatat gtataaatga aaaggtatat 3120gatgaggata ctaataaata tatagataca tccccaaatt tttcatataa tcataatatt 3180cagaatcact atatggtaga agaacattct gaggataaaa aaccgtatta tatgaataaa 3240ataaaatata taaaaaaaaa tgatgaattt tttgaagaac atatgaaaat gtatgagagc 3300atgcttatat ataatagatc caaaaaatca aatgataata ataccttacc tgttaataat 3360aaaatgggaa attcaaaagg aaaaaatttg gatcacgatg atggtgatca tgtcaaattg 3420ctttttggaa ttgacgacaa aaatgtaagc aataataata acacaaataa tataaatagt 3480ataaatagta taaataatat aaataatata aatgtgtgta ataataacaa tacatatgtt 3540tataataagg tggaatttaa atctatatgt gatcttttgt gtaaatattc atgcaaacaa 3600ttaaaagacc aagaaaatga atcaaaccca ctgttaagag aaaccgaagg gaggttaacc 3660tcggataata taaatttcaa caaattagat aataataatg ataatataca aagtgatttg 3720ttagataatg ataaaaggta ttataataaa ggatctgtaa ggaaagaatc aatatataat 3780gaagatagaa tatgtgaaaa atataaaaag agcaaactag aatggaatga atatttagaa 3840gaatttatga atatagaaat aagaaatcca tttattatat tatatcaatt aatatttaag 3900aattttcgta tgaatttaaa tataagaaaa ataaatgata tgtatgtaga aatcgaaata 3960catcatataa agatggcata taaaatattt agtgatatat atacaaataa atctactgta 4020gattctagga aaattggtca tatattttat tttcaatttt tttatgatat aattatacgt 4080atctttaatg aattatatta tttagaatat agtaatatac caagaaaaac atcttatgaa 4140aaatatatag aaagaggtaa atatcatgtt aagaaaatat taatacaatt attagcatca 4200aaagattttc aatattttca actcgaaaat gtatttccta agcaatttag acaccatcca 4260agtttttatg aacttgttaa taaatatgga tataaaacac atatgcctag aagtaatgtt 4320aatttgataa agttaaataa gaattcgtgg tatctctatg atgccttttg gcccctttca 4380gcattcaaag attttcaaac agctaatgaa aaatgtataa aagaagaaaa ttcttcctat 4440ataggatgtt caagggaaga agacaaagaa tatttaaatt tttcatttcg caaagtacaa 4500aatttattat tctatacatt taaaaatagt tgtctcgttt ctatactttt aattgtgttt 4560attattaatg acgaatttca gaggaaaatc gaaaaaaaaa aaaaagaaga attagaaaag 4620gaagaaatga aaaagcatat gaaagcggat gtaacgggag aagacgactc gttgcttaat 4680caaaaaggtg gtacaacgaa taataatacg tgtgatgtag gagttgtcat taatgaggaa 4740gcaaaccgat ccagtggaat gtcacaaaat gttgtgactt atgagagtgg tataaatatg 4800aataatgatg atgtattagg agaagaaagg aataataacg atgatattaa ttatatggat 4860gataatgatg atgataatga tgataatgat gatgataatg atgataatga tgatgataat 4920gatgatgata ataatgatga taataatgat aatgacaata atgatgatga caataatgat 4980gatgacaata atgatgatga caataatgat gatgacaata atgatgatga caataatgat 5040gatgacaata atgatgatga caataatgat gatgacaata atgatgatga caataatgat 5100gatgacaatg atgataatga tgaagattcc aatcttcatg gtagttcttc taatatgcta 5160aatattataa acagcggtga taataattca gtaactaatt ctagtaatat aaatacagca 5220cgaaataata atagtacata tataattgta gatcagaatg gagataatga aaccttattt 5280gcagagatat taataaatcg taatcctaat aattctataa atatggaaat tgtaaatgta 5340acggataata atgttggtga tgaaaataat aatagaaatt atcgtcattt aaatgctgat 5400ggattatctg aattaatcag aagtcttata agtgaaaata atattattaa taatgtaata 5460gatgaatctt ttttacaaaa taataatgta ggaggtgata attctattaa taatgatgac 5520ataagaaata gaggtggatc aatatttgat gataatatat cttttgataa taattcatca 5580agtgatgaat tgaatttaat ggattctaca aattcaaata atgtcagaat caatttatac 5640ggtagaacaa atggattgta cgatttaaat tttattttgg atagtaatga aattatgggt 5700aaccttaata cacataatgt gaataataca atagatatat tgatcaaaat tataaaaatt 5760ttgagtgtcg ccatgggttc agcgcatccc tttattgagc taaatagttt ttattatttt 5820aacgcggtga atttttatgg cacagataat gatgatgaca gtgaaaaaaa tgaggaaatt 5880ggtaacgaag aaaatgacaa aaaatatatt gataaaaaat atattgataa aaaatatatt 5940gataataatg aagaaattga aaaaaaacac cattgtgatc aaaataaaat ttatgatgat 6000gttgataggc tatcctccgt atcatcaaat tcttatgata tattaatgaa tcatttggaa 6060gaatcatgta tgtatacaag ttattcagat gaaacatatg agaagaatgg taatgtttat 6120aatatgaggg agggatttaa tgtggaaaac ctttcttatg ataaagatat attaaatgaa 6180aaaaaagaca tagtagaaat agaaaatgag aaagagaaat atataaaaat aaatgatcat 6240actgtgagta gaaattatgg ttgtgtttcc ttattaaaag atgaacctaa attttcatca 6300accgatggaa ataataataa tattattaat cgttgtaata ataataatta ttattatgat 6360aatatgaaac atgttgatga gaaaggtggt gaaggtgaag gtgaagattc agaagaatgt 6420caaataaaag aaagttacaa gaaaatgtct gaatgtaata ataaggaaaa tataattttt 6480gattccatta atgtattaag aaaaaacaat ataaagagat taaaaaatta tatgtgtaaa 6540aataaaaatt gttatatata ttatgatgat aataataata aaaagaagaa gaataaaaag 6600aatgttgaaa atcaagaaaa agaattttac gtgttaaata aaatatttgt acataatttt 6660ataaattgta ttaataatat aaatgtgaat gaagataagt gctttcaaaa agtgagatct 6720accattttaa atagattaaa agagatgtat tctggaaatt atgattgtaa aaataataat 6780agtaataatg aatttataga attagcaaaa aaaaaacaag aagatttatt aaaaagtatg 6840aaagaacaac aatccaaatt tagtcatttt ttagaagagg aatattcatc tgaagaaaat 6900gattcgctac caaatggagg aactgaagat tttgaagatg tcgattttgt ggatgatgct 6960agtagttatt tagatagtaa tagtaataat aatagtgatg gtcatgaaga tgataataaa 7020aatcgttgta ctgatgtaaa aacaaataaa caagaagaag aattctctat atctaatgtt 7080aatgaaaaaa aaaaaaaaaa atatatgaaa ggaataacaa tttgtgaata tgaaaaaata 7140aaagaaaaaa attgtatatg tgttttatgt aaagaaggga tgtctaaaaa taatttatta 7200gcatatatat gtttttcatc acatactaat ttattaaaaa aaattataaa aaaaaataaa 7260ttttcatttc cttgtaaaca cccatctata tatacgtgtg gtcattttat acatacaaaa 7320tgtttacata atacaaggat attaaaatat agaaaattgt tcagtgtaaa aaatgaacca 7380actctttatg aattcacatg tcctttatgt cgttcaatag ctaatagttt tgtggtgtat 7440ataccaaaga ggtatatatc ggagaatgat aaatatagaa tttatgattg taatgaggag 7500gaatttttct tggataactc aaataaaaat ttatccgaac ttttgaaaat ttcgaaatat 7560tcagaagaga ggaattatgg aattaaaagg aagaaggaaa gagaagaaaa aaaaaaaagt 7620aaaatgataa tgatgagagg aatggaaagc gataatataa acgaaatggt aagcgataat 7680ataaacgaaa tggcaagcga taatataaac gaaatggtaa gcgataatat aaacgaaatg 7740acaagcgata atataaacaa aatggcaaac caaatgaatt atgaacaaaa tacagatggt 7800attataataa aatcaagtga taatataaac gaaatgacaa gcgataatat aaacaaaatg 7860gcaaaccaaa tgaattatga acaaaataca gatggtatta taataaaatc aagtgatata 7920ccaaataatg ttcaggtaga catatttata aataataata tatcacaaat ttatgagtgt 7980gctgagcaaa acatagaatt attaaaagac ataaagaatg gagaatctac aaatatgtgc 8040aacaaagcag ataatttgtt tgctatacaa aatgataaca attatcctta tagaaataaa 8100gaaagtgaag aaatatgtga aggagaagat tttttattaa taaataaaaa gaatatttct 8160tgtaattatt tgaataacta tgtaacaaac gatacattaa gaaatgacaa tgtaatgata 8220cataaggata tcttattaga tgattatttt ttacaagatg ataataaatt ttctacatgt 8280tctataacat tgaatgatta tctaatagct cgatatttta atataacaaa ttgtgattat 8340attaaaaatg ataaatgtaa gagtaggaag gatatttgtt ttaatgataa taataataat 8400tacaataatg atgatgataa tgatgatgat aatgatgata ataatgatga tgataataat 8460aatgatggtg acaataatta cgatacgggg gaagaaagct tatccaccct gtcgtcttgc 8520tcatcagaaa atgtatcatc cgataatcat atggatgata gtgatatagc tgaagaattt 8580tattttaaat atacacatga taagaataaa aatataaaaa tggcgaacaa aaaaaataag 8640aaaaggaact taaaggaaaa tcataaagaa gatttccata tgaataatag taatactcat 8700aatgtgagtt catataattt tttcgatgaa aataagaaga atacaatttt taatttaaat 8760ttctgtaatc ttataaaaaa ggatagttta ttaaatgata tgttagcata tgaatgttat 8820gaaaaaaata aaaaaataat attaagaaat atttatttaa atgaaaataa tatgtatgat 8880gaattatttg aaaaaaagaa aaatgataat aatgtgggta cacataatat tatatattca 8940caagaaaata atgatgacct tttaaataat aaagatgata taaatattat atataaaaat 9000agcaatatgg aaaaacaaaa aagaataaga aggagaaaaa aaggaagagg taccgaaata 9060tatttttata aaaatacatc taattatgta aataaagatt atacatttac atatagaaat 9120tcgaattttt attatcgtac gaaaaatggt tttttatctt actatgttag tttaccatta 9180tcatttatta aagaaataca atattttatg ataaaaaata aattatataa ttacccagga 9240aataatagat ataataaatt ttatttaacg acggaggtag ataaggaaga aatcaaaaaa 9300gaagaaatgg agatgaatat cgatatgaaa atcgacccgt ctatgttttg taattttgaa 9360gacaaaataa aaacgaagga agaatataat actgtattat attatgaaat gaataatgat 9420attataacat atgtaaaatc taattttaat tatgaaagca taaatgccga tgaggcggtt 9480aaaaagtatg aggtatgtga tttttataca gatggtactt ttgatagtgc tggtgaaaaa 9540ttatgtagtg aagaaaagca agatcattta ataacgagac ataaaataaa agagtgttca 9600catgaaaatg aaagtgagca gaaggaaaaa agattaaaag taggacatga gcatgaaatg 9660gtggatggta taagtaatat gggcgtggct aataataatg ataatatata tggtgataat 9720atatatggtg ataatatata tggtgataat atatatggtg ataatgataa tatatatggt 9780gataatatat atgataataa taataatagt tgtgtagggg gtgaagattt tatttgtgat 9840aacaaatcag atgatattaa ttttgaattt ataaaagatt taaataaaat aaattatgaa 9900gaaaataaaa aagaaaaaaa agaaatgtta gaaatagaaa aattaaataa agagaatata 9960aatggtgata aaaaagatat atataaattt aaatatccat gtaatataga agaaataaaa 10020gatcataata tatcgatgaa aacaagttta tctatggatg tatatgaagg tattgtaagt 10080acaaataaaa atatatatga aatatacaat gaattaaaaa ggaaacaata tttttattcc 10140gtgtgcgata taaaatcatt aagacaatca attatattat tagatggtat atcggaaata 10200tcatggagaa tatttcatca agaacataca acattttcat ctatattaca ccattcggaa 10260aattataaaa gattaattga tttcgattat aatacaaaaa tagatgataa cgatgaagat 10320attgatataa aaggtattgg tgttaacgat ttattaaaaa tatatcgtag attagaaata 10380tatcctgatt ttatatttaa taataataac gtgggtttta ttaatagaca tgatttaaaa 10440tttataacta aaaaagatct ccttttaatg aatagaaaag attatgagag aaataaaaag 10500gaaaggaaaa aaaaaaatac taatttgtat ccaacaaatt cacaaactaa taataatgaa 10560aatgatgata acaataataa taacaataat aacaataata acaataataa caataataat 10620aataacaata ataataataa taatgatagt cgtggggatg ttaataaacc taagaataat 10680aattccttta ataataatag tgagacaaaa gaaggtaata aaaagaaaaa gaagaatcat 10740agtgatgtat taaacataaa atactggaga aatattaata cggctgttga tttttttaaa 10800atatattgga ttttatataa tgagatatta ataaatattt tttataaaaa tataaattat 10860atatcttgta ataatatgat agaaatttta gtacgtaatt tatttttata caaatatgaa 10920ttaaatattt taaaagaaga aaatatgtgt aatcttttta atattcaaac atggaaaaat 10980atgtataaaa tatgtttctt ttatgtctat ccttatcgat ttacctttaa taaatataaa 11040aaatttttca ataaaattaa aaaaattcac tttttaaata atttatcctt tattccatta 11100tcagtagatg tattaaaatt atttatgaat ttttttttaa atcaatattt atatactcct 11160tcattaatta agcattttat ttcaatatca ttaatcataa tatcttttca aattatgaat 11220gaaatattta taaaagaaat atcaaagaca tatgtaagat ttatttttaa gacaatacaa 11280aattataaca aaatcaagaa atatattact cgtatatcta aatatatgta ttttcaaaaa 11340atatatacaa attatattat cagccattat gaatatgtag actatattga gagggccaat 11400acatttattc aaaccgaaga aacaaaaaaa ataagacata atgaaagttc gatttatcat 11460aatattgaaa tggtaggaca taaacaaaat gaacagaaaa ggcattcttt aaattcttac 11520gacagatttc acatttttct ttttgaaaaa tatgtaaaaa attctatacc aggaggtgaa 11580aatataactt taaaatatgt gaatggtcga tataaattat atgacaatga gcaagaggta 11640gtacgtatga gtgatcttga acattttgaa aagataaaaa atgtttattc gaaaaaagca 11700acggatattt taaaaaagaa gaatgaaaaa atgaaaaatg ataaagagga aactggtaat 11760gaacaagcaa ataattgtaa taaaacatta gatggtaata aaacattaga tggtaataaa 11820atattagatg gtaataaaac attagatggt aataaaatat tagatggtaa taaaatatta 11880gatgataata aattattaga tgataataaa ttattagatg gtaataaaac attagatggt 11940aataaaatat tagatgataa taaaatatta gatagtaata ataataataa taataataat 12000aataataatg gcgaaaacaa aagttggaac ttccagaatg tatcatctta caacgattta 12060ataaaatccg tgcttgataa aaaaaatttc ttttttatta tagatattgt taacttattt 12120aaaaatatac aaacattgtc gtatttattt gatgaagaaa caataaggta cgtacacatg 12180attattatga atagtaaaat attatcatat gatttaaaag aaatagaaag aaaaaaaaac 12240ttgcaaaaaa agatttatcc aaattttact tcttatatta caaaacagaa gaaggcaaat 12300aaattaaaat gttataatta tgatttatta catattgaaa agaaatggat attatatctt 12360atatataaaa ccatattaaa atattatgat atgcttatat ttaagaagaa agttcaaaaa 12420cttatgcttt tgatgtgtga aattaatgat gaatatataa aatatatata ttttaatggt 12480tatattccta taaatatata tttaagtaca aacttaataa aatacaatga attctttaca 12540aattattatt cttatatata taacaaaaat gttaatgata aatggggtga tttccatgtc 12600ttatatttcc aaaatgaaga aaataatata aaaaaagaaa acaaaaaaaa ttattattat 12660tattattatt attattatta tacacttttt aaaaacaaac atgttttaaa aagagattat 12720aatcatattg taagtaataa tatacaaccc attaataaaa tgaatgaacc cataacatct 12780aatgaatgta cctctccaaa tatgtcttat catatagaat caaatgaaat atttaaaaat 12840gaagatgatc ataatcttaa tatatataat gaacatatta tgtataattc gcacaaagat 12900acaaatttgt tatcttcctc taaccatatg caacacgtac aaaaaaatac cctcaccatg 12960aatcttgatc attccaataa tctaataata aatgataacc ataaaagaga taaagaacat 13020aatataaata cgaaacattg gaataagaac ataataaata gtaattattt taatgtatat 13080aataatgatt ctagcccaaa ggagcaaatc catttagagg aaggtggggg tggtttaata 13140gatgttgatg atgataaaaa ggataatgtt attaatagtg ataatcattt taaggaaacg 13200acatataatc atatagcata tcataaggac caagaattat ccacagaatt aaatttattt 13260aaatataaat tatataatat atccagtgaa gaaattagta atttatattt aatggaagat 13320tgtaataatg aaaaaggatt tttttcatgt gttcataaaa atgaagattt tgaatataag 13380aatattaaca ttttaaatat attaaaatat caatttgaac tagttccata tattaatcat 13440ttatattatc tttataatat ctatccatta ttatttaaat tgaaagagat aaaaaatatt 13500catgatatga aagaaaaaca aattaattta tgtacagacc aatttttatt atccttagta 13560gataaagata tttatgattg ttatgatgat gtaaattttt ctgatgctag tttttatagt 13620gatcaaacta ttcatgtttc tgaaaaagaa tgtgaacata aaaatcataa tagtaataaa 13680tatgattgta ataattatca tccttttaat gtatctcatt tatcttatcc atataaagat 13740caaaatggtg acgaagaaga tacattgcaa attaattctt cattaaatat tcttgaaaat 13800gttgtcgatc ttgatgaact cataaaagat ttgaaaaaaa caaaaaagag caaaaaaaaa 13860actagtaaaa ataaatatgc tgataaattt tttgtctcat tttttaatac agcattgaaa 13920agagacgaag ataaaggaaa aacaaattat gtacattata gaaaattacg aaatgctaaa 13980aataatataa aattaaaaaa tttattctta tcacaccaac aaaatgatac tatttatgat 14040aatataaatg gtaatatttg tgataattta tacggtaatg tcaataccta tcaaaactca 14100gcttctctta tatttgtaga tccacataat ttaacaaata taaatatttc caacgaatta 14160aataataata tcaatataaa ttgttcgaaa aataaaaaaa aaaaaaaacc atcatcaaat 14220gatcaaaata atactgaaaa tgaagacatg caaaatagct catctgataa tccatatgat 14280gattcaaatt ttatatctta tcatgaaagt atagattcta caagtagtac agataaatat 14340cataaaagaa aaatgacccc tatggaaagt atgtttttaa aatatacatt aaatacatca 14400gaagatgcat tatccaataa atataatgta actagcaaaa ataaaaataa aaaagaatat 14460ttaagttttc ttaaatataa agatgcaaac aaaattcaac gttatcatat gttattaaga 14520aaaaatggtc ttggaagatt gtttttttat aatttaagaa aaatgtataa ttatgtatat 14580aatctatatg atttcatacc tctttatctt atacaaaata tattaataca ttattatgat 14640atatatcaac atgtaaacca atattattat catgatgata aagaatatcc cacttttttt 14700acacaacaaa atgaagatga tgaatatgaa tataccaagt tttctaaaaa tttctttaat 14760actaataaat ggaaaaaaat gtattcgaaa tatgaagaca aactaaataa cgaaacatat 14820ccggaaccac caaaaaaata tatttcaaat aaacagcgac ataaagaaaa tattctaata 14880taccaaaatt tgcaaaatga tcatatgaat aatcattata atattgctaa taatgatata 14940gaaaataaaa atcaaaccaa ttcatcatac ctttttaatg ttcaccatca aaattgccct 15000tctctatcat caaacaatct tgtaaatatt aatagtactt taatcaatga ccaccatttt 15060gtacaaacaa ataatgatca tatgaacatc atacaacata tggagaacat tccagaaatg 15120tcggaatgta atttaaaaac tgaccaaaat aataacatct ttaattttcc acaagataaa 15180ataaatcctg atacaatgaa gaaaaatgaa aaaatggaat tctcggtaaa taaaaaaata 15240aaaacaaacc ataacacaac tggatataat aaaagaaata atgataggta taattattct 15300agtactccta atttttttgg aaaagatgaa tatgataaca catcatatga ggattatata 15360aaaaaaaaaa aaaaaaactt ctcttcaaca agtagaagaa aacgaaataa taatataaat 15420atgaaagaat tttttgcaaa tggacgttat gataaattat taagtataaa gaaaatattt 15480atgatattct ataattattt gaggaagtgt cattttatcg ataaaaaaaa ttcttgtaag 15540aatttatata aatcattttt aaaaaaatat ttaaaatcag ctaatgtata tttacaattt 15600atagcttata ttatattctc catatatgat tataattcat tagcaaaaaa atatttagat 15660tatatacact tatatagtga tattagtaaa tataatatat tccttcatat attaaatatc 15720gacgaagact atgataaaat atatatatat caaacattac aattaatata tcatccatat 15780ttttatctaa tgtataaatt atataaatat

ctaaaaatag atgcctcccc agtattatta 15840catgattttt atgaaaactg ggaagaattt acaaaatccc catctttatt tcaacctagc 15900aaaaattatt tcacattaat taaaaaacat ttcaaaagaa aatgtgtaag ttgtaataag 15960tctccaaaaa aaatacttat ctgtctctat tgtggttcaa ctgtttgtct gcatgaaggt 16020gaccatgtgt ccggaccctt atcacaaact ataagtaaat gtgtgtacca tacgaccatc 16080tgtggaggag agcaatgttt atacttatgt ttaaatacat catcggtgtt gtttacgagt 16140gaaaaccgat tcgattttat gagtggacct tatgtcgaca aaaatggaga tgtggactat 16200caattaaaga gaggaaaaaa tttatacctt tcttcttata agttgaacaa actttttgac 16260gttataatta attcagcagt tgatgttgaa atatataagc atacgttgaa atcggaataa 16320105439PRTPlasmodium falciparum 10Met Glu Pro Lys Glu Thr Asp Asn Val Ile Lys Asp Glu Asp Asn Val 1 5 10 15 Lys Lys Glu Glu Pro Ile Leu Ser Ile Glu Lys Tyr Leu Leu Lys Glu 20 25 30 Phe Gly Asp Gly Lys Thr Asn Lys Lys Asp Val Met Arg Lys Leu Arg 35 40 45 Lys Arg Ile Tyr Asn His Lys Asn Ser Glu Glu Ile Tyr Lys Lys Leu 50 55 60 Lys Glu Ile His Gly Asn Ser Gly Ile Cys Thr Arg Gln Trp Lys Glu 65 70 75 80 Gly Ser Phe Ala Phe Lys Cys Tyr Asn Cys Glu Gly Asp Pro Thr Cys 85 90 95 Ala Ile Cys Ala Lys Cys Phe Phe Ala Ser Asn His Lys Asn His Ile 100 105 110 Tyr Arg Leu Thr His Thr Ser Gly Gly Cys Cys Asp Cys Gly Asp Thr 115 120 125 Ser Trp Asn Ile Asn Gly Ser Cys Phe Asp His Arg Gly Ile Asn Glu 130 135 140 Asp Cys Leu Ile Asn Asn Ser Ile Leu Asn Ile Asp Ile Lys Asn Arg 145 150 155 160 Val Arg Glu Asp Leu Glu Cys Leu Ile Gly Thr Leu Phe Arg Asp Ile 165 170 175 Val Leu Lys Asp Gly Tyr Phe Leu Ser Leu Val Ser Ala Asp Tyr Ile 180 185 190 Glu His Ala Phe Asp Phe Phe Lys Asp Leu Gly Ile Thr Ser Tyr Leu 195 200 205 Phe Arg Gln Val Ile Cys Glu Val Phe Thr Gly Ala Lys Ile Asp Phe 210 215 220 Leu Val Asn Phe His Tyr Cys Phe Tyr Val Gly Val Gln Lys Ala Leu 225 230 235 240 Tyr Ser Phe Tyr Leu Ser Leu Phe Val His Pro Tyr Phe Lys Gln Arg 245 250 255 Phe Ala Phe Lys Leu Ala Lys His Tyr Lys Leu Val Val Arg Val Ala 260 265 270 Asp Asp Arg Ile Tyr Asn Asp Asn His Leu Asn Gly Leu Ser Val Gln 275 280 285 Val Leu Thr Val Pro Glu Ile Ala Tyr Thr Leu Val Ile Asn Asn Phe 290 295 300 Leu Asn Asp Leu Leu Lys Leu Ile Glu Asn Leu Cys Val Phe Asn Asn 305 310 315 320 Val Thr Asn Cys Val Met His Lys Asn Phe Leu Arg Ser Asp Ile Glu 325 330 335 Ile Ile Ile Arg Ile Cys Val Asp Leu Lys Tyr Leu Leu Tyr His His 340 345 350 Glu Val Ile Lys Ile Val Leu Phe Asn Lys Tyr Val Ile Lys Lys Ile 355 360 365 Leu Lys Ile Leu Ser Leu Leu His Arg Met Asn Ser Gln Val Arg Tyr 370 375 380 Thr Lys Ser His Ile Ile Tyr Glu Asp Leu Ser Ser Ser Tyr Ser Ile 385 390 395 400 Thr Ile Glu Asn Tyr Leu Leu Arg Val Phe Glu Pro Leu Ala Asn Phe 405 410 415 Cys Lys Arg Glu Ala Asn Ala Asn Phe Arg Asn Leu Phe Leu Leu Tyr 420 425 430 Gln Leu Thr Ile Arg Gly Ile His Lys Leu Asp Leu Phe Pro Phe Asn 435 440 445 Arg Lys Lys Lys Glu Ala Glu Asn Ser Phe Met Asn Arg Lys Lys Leu 450 455 460 Lys Glu Lys Lys Ile Thr Lys Lys Arg Asn Ile Lys Glu Asp Asn Ser 465 470 475 480 Thr Asp Asp Leu Thr Gly Asp Ser Asp Asn Ser Ser Thr Asn Asn Val 485 490 495 Cys Arg Cys Asp Thr Ser Asn Tyr Asp Asn Thr Met Phe Asp Arg Ser 500 505 510 Cys Ser Ile Pro Gly Leu Asn Asn Met Asn Asp Asp Val Asn Lys Ser 515 520 525 Gly Gly Val Ser Leu Asn Asp Asp Asn Ile Asn Asn Asp Asn Asn Asn 530 535 540 Thr Asn Asn Asn Thr Asn Asn Asn Thr Asn Asn Asn Asn Asn Asn Thr 545 550 555 560 Asn Asn Asn Thr Asn Asn Asn Asn Asn Asn Lys Asn Cys Glu Gly Lys 565 570 575 Ala Asn Asp Gln Thr Asn Lys Lys Lys Lys Phe Lys His Leu Arg Ser 580 585 590 Leu His Ser Pro Leu Val Arg Phe Phe Ile Met Ile Leu Asn Phe Asp 595 600 605 Val Ile Lys Lys Cys Leu Asp Asp Val Tyr Trp Tyr Lys Tyr Leu Tyr 610 615 620 Leu Arg Arg Val Tyr Glu Lys Lys Arg Ile Tyr Lys Asn Arg Lys Lys 625 630 635 640 Ser Pro Ala Tyr Asn Thr Asn Asp Asp Asn Lys Asn Glu Val Cys Thr 645 650 655 Lys Asn Ile Pro Cys Asp Asp Asn Lys Asn Glu Met Cys Thr Lys Asn 660 665 670 Ile Pro Cys Asp Asp Asn Lys Asn Glu Val Cys Thr Lys Asn Ile Pro 675 680 685 Cys Asp Asp Asn Lys Asn Glu Val Cys Thr Lys Asn Ile Pro Cys Asp 690 695 700 Asp Asn Lys Asn Glu Met Cys Thr Lys Asn Ile Pro Cys Asp Asp Asn 705 710 715 720 Lys Asn Ile Pro Arg Asp Asp Asn Lys Ser Tyr Asn Lys Met Tyr Asn 725 730 735 Asn Asn Lys Met Asn Lys Glu Leu Thr Tyr Asn Asp Glu Tyr Ile Ile 740 745 750 Asn Glu Phe Ile Arg Asp Phe Tyr Ser Ile Glu Lys His Ser Glu Glu 755 760 765 Ile Ile Asn Gln Arg Lys Ile Leu Gln Glu Arg Tyr Glu Asn Asp Gly 770 775 780 Asn Asn Met Lys Ile Thr Leu Phe His Phe Lys Lys Phe Phe Thr Ser 785 790 795 800 Asn Asp Glu His Lys Asn Glu His Leu Gly Lys Tyr Ile Asp Glu His 805 810 815 Ile Tyr Leu Glu Ser Asn Tyr Phe Lys Trp Ile Met Asn Asn Asn Ser 820 825 830 Ser Ser Lys Leu Arg Glu Cys Asn Asn Lys Tyr Arg Ile Lys Tyr Arg 835 840 845 Glu Thr Ile Gln Glu Lys Asn Ile Lys Ile Lys Cys Lys Asn Ile Tyr 850 855 860 Ile Glu Lys Lys Leu Lys Asn Ser Lys Tyr Glu Phe Asn Glu Phe Leu 865 870 875 880 Asp Ile Lys Tyr Asp Lys Glu Lys His Asn Ile Tyr Leu His Pro Leu 885 890 895 Tyr Ile Asp Leu Tyr Met Arg Asp Ile Phe Glu Phe Leu Asn Leu Phe 900 905 910 Asp Leu Lys Leu Leu Lys Asn Ile Leu Phe Ile Asn Ile Gln Ile Leu 915 920 925 Thr Phe Ile Tyr Glu Ile Lys Tyr Gly Tyr Trp Val Arg Asn Gly Pro 930 935 940 Gln Met Ile Phe Gln Val Gly Glu Tyr Asp Asn Ser Leu Phe Phe Gln 945 950 955 960 Asn Asp Leu Ile Gly Ile Gln Phe Ala Ile Ile Met Met Asn Val Leu 965 970 975 Pro Leu Tyr Glu Asn Gln Lys Val Tyr Leu Asp Ser His Val Ile Asn 980 985 990 Leu Phe His Gln Ile Trp Asn Ile Lys Val Gln Tyr Asp Glu Pro Leu 995 1000 1005 Lys Lys Leu Pro Trp Ser Lys Asn Glu Tyr Asn Asp Val Gln Asn 1010 1015 1020 Asp Tyr Ile His Lys Ala Asp Asp Glu Ile Cys Ile Asn Glu Lys 1025 1030 1035 Val Tyr Asp Glu Asp Thr Asn Lys Tyr Ile Asp Thr Ser Pro Asn 1040 1045 1050 Phe Ser Tyr Asn His Asn Ile Gln Asn His Tyr Met Val Glu Glu 1055 1060 1065 His Ser Glu Asp Lys Lys Pro Tyr Tyr Met Asn Lys Ile Lys Tyr 1070 1075 1080 Ile Lys Lys Asn Asp Glu Phe Phe Glu Glu His Met Lys Met Tyr 1085 1090 1095 Glu Ser Met Leu Ile Tyr Asn Arg Ser Lys Lys Ser Asn Asp Asn 1100 1105 1110 Asn Thr Leu Pro Val Asn Asn Lys Met Gly Asn Ser Lys Gly Lys 1115 1120 1125 Asn Leu Asp His Asp Asp Gly Asp His Val Lys Leu Leu Phe Gly 1130 1135 1140 Ile Asp Asp Lys Asn Val Ser Asn Asn Asn Asn Thr Asn Asn Ile 1145 1150 1155 Asn Ser Ile Asn Ser Ile Asn Asn Ile Asn Asn Ile Asn Val Cys 1160 1165 1170 Asn Asn Asn Asn Thr Tyr Val Tyr Asn Lys Val Glu Phe Lys Ser 1175 1180 1185 Ile Cys Asp Leu Leu Cys Lys Tyr Ser Cys Lys Gln Leu Lys Asp 1190 1195 1200 Gln Glu Asn Glu Ser Asn Pro Leu Leu Arg Glu Thr Glu Gly Arg 1205 1210 1215 Leu Thr Ser Asp Asn Ile Asn Phe Asn Lys Leu Asp Asn Asn Asn 1220 1225 1230 Asp Asn Ile Gln Ser Asp Leu Leu Asp Asn Asp Lys Arg Tyr Tyr 1235 1240 1245 Asn Lys Gly Ser Val Arg Lys Glu Ser Ile Tyr Asn Glu Asp Arg 1250 1255 1260 Ile Cys Glu Lys Tyr Lys Lys Ser Lys Leu Glu Trp Asn Glu Tyr 1265 1270 1275 Leu Glu Glu Phe Met Asn Ile Glu Ile Arg Asn Pro Phe Ile Ile 1280 1285 1290 Leu Tyr Gln Leu Ile Phe Lys Asn Phe Arg Met Asn Leu Asn Ile 1295 1300 1305 Arg Lys Ile Asn Asp Met Tyr Val Glu Ile Glu Ile His His Ile 1310 1315 1320 Lys Met Ala Tyr Lys Ile Phe Ser Asp Ile Tyr Thr Asn Lys Ser 1325 1330 1335 Thr Val Asp Ser Arg Lys Ile Gly His Ile Phe Tyr Phe Gln Phe 1340 1345 1350 Phe Tyr Asp Ile Ile Ile Arg Ile Phe Asn Glu Leu Tyr Tyr Leu 1355 1360 1365 Glu Tyr Ser Asn Ile Pro Arg Lys Thr Ser Tyr Glu Lys Tyr Ile 1370 1375 1380 Glu Arg Gly Lys Tyr His Val Lys Lys Ile Leu Ile Gln Leu Leu 1385 1390 1395 Ala Ser Lys Asp Phe Gln Tyr Phe Gln Leu Glu Asn Val Phe Pro 1400 1405 1410 Lys Gln Phe Arg His His Pro Ser Phe Tyr Glu Leu Val Asn Lys 1415 1420 1425 Tyr Gly Tyr Lys Thr His Met Pro Arg Ser Asn Val Asn Leu Ile 1430 1435 1440 Lys Leu Asn Lys Asn Ser Trp Tyr Leu Tyr Asp Ala Phe Trp Pro 1445 1450 1455 Leu Ser Ala Phe Lys Asp Phe Gln Thr Ala Asn Glu Lys Cys Ile 1460 1465 1470 Lys Glu Glu Asn Ser Ser Tyr Ile Gly Cys Ser Arg Glu Glu Asp 1475 1480 1485 Lys Glu Tyr Leu Asn Phe Ser Phe Arg Lys Val Gln Asn Leu Leu 1490 1495 1500 Phe Tyr Thr Phe Lys Asn Ser Cys Leu Val Ser Ile Leu Leu Ile 1505 1510 1515 Val Phe Ile Ile Asn Asp Glu Phe Gln Arg Lys Ile Glu Lys Lys 1520 1525 1530 Lys Lys Glu Glu Leu Glu Lys Glu Glu Met Lys Lys His Met Lys 1535 1540 1545 Ala Asp Val Thr Gly Glu Asp Asp Ser Leu Leu Asn Gln Lys Gly 1550 1555 1560 Gly Thr Thr Asn Asn Asn Thr Cys Asp Val Gly Val Val Ile Asn 1565 1570 1575 Glu Glu Ala Asn Arg Ser Ser Gly Met Ser Gln Asn Val Val Thr 1580 1585 1590 Tyr Glu Ser Gly Ile Asn Met Asn Asn Asp Asp Val Leu Gly Glu 1595 1600 1605 Glu Arg Asn Asn Asn Asp Asp Ile Asn Tyr Met Asp Asp Asn Asp 1610 1615 1620 Asp Asp Asn Asp Asp Asn Asp Asp Asp Asn Asp Asp Asn Asp Asp 1625 1630 1635 Asp Asn Asp Asp Asp Asn Asn Asp Asp Asn Asn Asp Asn Asp Asn 1640 1645 1650 Asn Asp Asp Asp Asn Asn Asp Asp Asp Asn Asn Asp Asp Asp Asn 1655 1660 1665 Asn Asp Asp Asp Asn Asn Asp Asp Asp Asn Asn Asp Asp Asp Asn 1670 1675 1680 Asn Asp Asp Asp Asn Asn Asp Asp Asp Asn Asn Asp Asp Asp Asn 1685 1690 1695 Asn Asp Asp Asp Asn Asp Asp Asn Asp Glu Asp Ser Asn Leu His 1700 1705 1710 Gly Ser Ser Ser Asn Met Leu Asn Ile Ile Asn Ser Gly Asp Asn 1715 1720 1725 Asn Ser Val Thr Asn Ser Ser Asn Ile Asn Thr Ala Arg Asn Asn 1730 1735 1740 Asn Ser Thr Tyr Ile Ile Val Asp Gln Asn Gly Asp Asn Glu Thr 1745 1750 1755 Leu Phe Ala Glu Ile Leu Ile Asn Arg Asn Pro Asn Asn Ser Ile 1760 1765 1770 Asn Met Glu Ile Val Asn Val Thr Asp Asn Asn Val Gly Asp Glu 1775 1780 1785 Asn Asn Asn Arg Asn Tyr Arg His Leu Asn Ala Asp Gly Leu Ser 1790 1795 1800 Glu Leu Ile Arg Ser Leu Ile Ser Glu Asn Asn Ile Ile Asn Asn 1805 1810 1815 Val Ile Asp Glu Ser Phe Leu Gln Asn Asn Asn Val Gly Gly Asp 1820 1825 1830 Asn Ser Ile Asn Asn Asp Asp Ile Arg Asn Arg Gly Gly Ser Ile 1835 1840 1845 Phe Asp Asp Asn Ile Ser Phe Asp Asn Asn Ser Ser Ser Asp Glu 1850 1855 1860 Leu Asn Leu Met Asp Ser Thr Asn Ser Asn Asn Val Arg Ile Asn 1865 1870 1875 Leu Tyr Gly Arg Thr Asn Gly Leu Tyr Asp Leu Asn Phe Ile Leu 1880 1885 1890 Asp Ser Asn Glu Ile Met Gly Asn Leu Asn Thr His Asn Val Asn 1895 1900 1905 Asn Thr Ile Asp Ile Leu Ile Lys Ile Ile Lys Ile Leu Ser Val 1910 1915 1920 Ala Met Gly Ser Ala His Pro Phe Ile Glu Leu Asn Ser Phe Tyr 1925 1930 1935 Tyr Phe Asn Ala Val Asn Phe Tyr Gly Thr Asp Asn Asp Asp Asp 1940 1945 1950 Ser Glu Lys Asn Glu Glu Ile Gly Asn Glu Glu Asn Asp Lys Lys 1955 1960 1965 Tyr Ile Asp Lys Lys Tyr Ile Asp Lys Lys Tyr Ile Asp Asn Asn 1970 1975 1980 Glu Glu Ile Glu Lys Lys His His Cys Asp Gln Asn Lys Ile Tyr 1985 1990 1995 Asp Asp Val Asp Arg Leu Ser Ser Val Ser Ser Asn Ser Tyr Asp 2000 2005 2010 Ile Leu Met Asn His Leu Glu Glu Ser Cys Met Tyr Thr Ser Tyr 2015 2020 2025 Ser Asp Glu Thr Tyr Glu Lys Asn Gly Asn Val Tyr Asn Met Arg 2030 2035 2040 Glu Gly Phe Asn Val Glu Asn Leu Ser Tyr Asp Lys Asp Ile Leu 2045 2050 2055 Asn Glu Lys Lys Asp Ile Val Glu Ile Glu Asn Glu Lys Glu Lys 2060 2065 2070 Tyr Ile Lys Ile Asn Asp His Thr Val Ser Arg Asn Tyr Gly Cys 2075 2080 2085 Val Ser Leu Leu Lys Asp Glu Pro Lys Phe Ser Ser Thr Asp Gly 2090 2095 2100 Asn Asn Asn Asn Ile Ile Asn Arg Cys Asn Asn Asn Asn Tyr Tyr 2105 2110 2115 Tyr Asp Asn Met Lys His Val Asp Glu Lys Gly Gly Glu Gly Glu 2120 2125 2130 Gly Glu Asp Ser Glu Glu Cys Gln Ile Lys Glu Ser Tyr Lys Lys 2135 2140 2145 Met Ser Glu Cys Asn Asn Lys Glu Asn Ile Ile Phe Asp Ser Ile 2150 2155 2160 Asn Val Leu Arg Lys

Asn Asn Ile Lys Arg Leu Lys Asn Tyr Met 2165 2170 2175 Cys Lys Asn Lys Asn Cys Tyr Ile Tyr Tyr Asp Asp Asn Asn Asn 2180 2185 2190 Lys Lys Lys Lys Asn Lys Lys Asn Val Glu Asn Gln Glu Lys Glu 2195 2200 2205 Phe Tyr Val Leu Asn Lys Ile Phe Val His Asn Phe Ile Asn Cys 2210 2215 2220 Ile Asn Asn Ile Asn Val Asn Glu Asp Lys Cys Phe Gln Lys Val 2225 2230 2235 Arg Ser Thr Ile Leu Asn Arg Leu Lys Glu Met Tyr Ser Gly Asn 2240 2245 2250 Tyr Asp Cys Lys Asn Asn Asn Ser Asn Asn Glu Phe Ile Glu Leu 2255 2260 2265 Ala Lys Lys Lys Gln Glu Asp Leu Leu Lys Ser Met Lys Glu Gln 2270 2275 2280 Gln Ser Lys Phe Ser His Phe Leu Glu Glu Glu Tyr Ser Ser Glu 2285 2290 2295 Glu Asn Asp Ser Leu Pro Asn Gly Gly Thr Glu Asp Phe Glu Asp 2300 2305 2310 Val Asp Phe Val Asp Asp Ala Ser Ser Tyr Leu Asp Ser Asn Ser 2315 2320 2325 Asn Asn Asn Ser Asp Gly His Glu Asp Asp Asn Lys Asn Arg Cys 2330 2335 2340 Thr Asp Val Lys Thr Asn Lys Gln Glu Glu Glu Phe Ser Ile Ser 2345 2350 2355 Asn Val Asn Glu Lys Lys Lys Lys Lys Tyr Met Lys Gly Ile Thr 2360 2365 2370 Ile Cys Glu Tyr Glu Lys Ile Lys Glu Lys Asn Cys Ile Cys Val 2375 2380 2385 Leu Cys Lys Glu Gly Met Ser Lys Asn Asn Leu Leu Ala Tyr Ile 2390 2395 2400 Cys Phe Ser Ser His Thr Asn Leu Leu Lys Lys Ile Ile Lys Lys 2405 2410 2415 Asn Lys Phe Ser Phe Pro Cys Lys His Pro Ser Ile Tyr Thr Cys 2420 2425 2430 Gly His Phe Ile His Thr Lys Cys Leu His Asn Thr Arg Ile Leu 2435 2440 2445 Lys Tyr Arg Lys Leu Phe Ser Val Lys Asn Glu Pro Thr Leu Tyr 2450 2455 2460 Glu Phe Thr Cys Pro Leu Cys Arg Ser Ile Ala Asn Ser Phe Val 2465 2470 2475 Val Tyr Ile Pro Lys Arg Tyr Ile Ser Glu Asn Asp Lys Tyr Arg 2480 2485 2490 Ile Tyr Asp Cys Asn Glu Glu Glu Phe Phe Leu Asp Asn Ser Asn 2495 2500 2505 Lys Asn Leu Ser Glu Leu Leu Lys Ile Ser Lys Tyr Ser Glu Glu 2510 2515 2520 Arg Asn Tyr Gly Ile Lys Arg Lys Lys Glu Arg Glu Glu Lys Lys 2525 2530 2535 Lys Ser Lys Met Ile Met Met Arg Gly Met Glu Ser Asp Asn Ile 2540 2545 2550 Asn Glu Met Val Ser Asp Asn Ile Asn Glu Met Ala Ser Asp Asn 2555 2560 2565 Ile Asn Glu Met Val Ser Asp Asn Ile Asn Glu Met Thr Ser Asp 2570 2575 2580 Asn Ile Asn Lys Met Ala Asn Gln Met Asn Tyr Glu Gln Asn Thr 2585 2590 2595 Asp Gly Ile Ile Ile Lys Ser Ser Asp Asn Ile Asn Glu Met Thr 2600 2605 2610 Ser Asp Asn Ile Asn Lys Met Ala Asn Gln Met Asn Tyr Glu Gln 2615 2620 2625 Asn Thr Asp Gly Ile Ile Ile Lys Ser Ser Asp Ile Pro Asn Asn 2630 2635 2640 Val Gln Val Asp Ile Phe Ile Asn Asn Asn Ile Ser Gln Ile Tyr 2645 2650 2655 Glu Cys Ala Glu Gln Asn Ile Glu Leu Leu Lys Asp Ile Lys Asn 2660 2665 2670 Gly Glu Ser Thr Asn Met Cys Asn Lys Ala Asp Asn Leu Phe Ala 2675 2680 2685 Ile Gln Asn Asp Asn Asn Tyr Pro Tyr Arg Asn Lys Glu Ser Glu 2690 2695 2700 Glu Ile Cys Glu Gly Glu Asp Phe Leu Leu Ile Asn Lys Lys Asn 2705 2710 2715 Ile Ser Cys Asn Tyr Leu Asn Asn Tyr Val Thr Asn Asp Thr Leu 2720 2725 2730 Arg Asn Asp Asn Val Met Ile His Lys Asp Ile Leu Leu Asp Asp 2735 2740 2745 Tyr Phe Leu Gln Asp Asp Asn Lys Phe Ser Thr Cys Ser Ile Thr 2750 2755 2760 Leu Asn Asp Tyr Leu Ile Ala Arg Tyr Phe Asn Ile Thr Asn Cys 2765 2770 2775 Asp Tyr Ile Lys Asn Asp Lys Cys Lys Ser Arg Lys Asp Ile Cys 2780 2785 2790 Phe Asn Asp Asn Asn Asn Asn Tyr Asn Asn Asp Asp Asp Asn Asp 2795 2800 2805 Asp Asp Asn Asp Asp Asn Asn Asp Asp Asp Asn Asn Asn Asp Gly 2810 2815 2820 Asp Asn Asn Tyr Asp Thr Gly Glu Glu Ser Leu Ser Thr Leu Ser 2825 2830 2835 Ser Cys Ser Ser Glu Asn Val Ser Ser Asp Asn His Met Asp Asp 2840 2845 2850 Ser Asp Ile Ala Glu Glu Phe Tyr Phe Lys Tyr Thr His Asp Lys 2855 2860 2865 Asn Lys Asn Ile Lys Met Ala Asn Lys Lys Asn Lys Lys Arg Asn 2870 2875 2880 Leu Lys Glu Asn His Lys Glu Asp Phe His Met Asn Asn Ser Asn 2885 2890 2895 Thr His Asn Val Ser Ser Tyr Asn Phe Phe Asp Glu Asn Lys Lys 2900 2905 2910 Asn Thr Ile Phe Asn Leu Asn Phe Cys Asn Leu Ile Lys Lys Asp 2915 2920 2925 Ser Leu Leu Asn Asp Met Leu Ala Tyr Glu Cys Tyr Glu Lys Asn 2930 2935 2940 Lys Lys Ile Ile Leu Arg Asn Ile Tyr Leu Asn Glu Asn Asn Met 2945 2950 2955 Tyr Asp Glu Leu Phe Glu Lys Lys Lys Asn Asp Asn Asn Val Gly 2960 2965 2970 Thr His Asn Ile Ile Tyr Ser Gln Glu Asn Asn Asp Asp Leu Leu 2975 2980 2985 Asn Asn Lys Asp Asp Ile Asn Ile Ile Tyr Lys Asn Ser Asn Met 2990 2995 3000 Glu Lys Gln Lys Arg Ile Arg Arg Arg Lys Lys Gly Arg Gly Thr 3005 3010 3015 Glu Ile Tyr Phe Tyr Lys Asn Thr Ser Asn Tyr Val Asn Lys Asp 3020 3025 3030 Tyr Thr Phe Thr Tyr Arg Asn Ser Asn Phe Tyr Tyr Arg Thr Lys 3035 3040 3045 Asn Gly Phe Leu Ser Tyr Tyr Val Ser Leu Pro Leu Ser Phe Ile 3050 3055 3060 Lys Glu Ile Gln Tyr Phe Met Ile Lys Asn Lys Leu Tyr Asn Tyr 3065 3070 3075 Pro Gly Asn Asn Arg Tyr Asn Lys Phe Tyr Leu Thr Thr Glu Val 3080 3085 3090 Asp Lys Glu Glu Ile Lys Lys Glu Glu Met Glu Met Asn Ile Asp 3095 3100 3105 Met Lys Ile Asp Pro Ser Met Phe Cys Asn Phe Glu Asp Lys Ile 3110 3115 3120 Lys Thr Lys Glu Glu Tyr Asn Thr Val Leu Tyr Tyr Glu Met Asn 3125 3130 3135 Asn Asp Ile Ile Thr Tyr Val Lys Ser Asn Phe Asn Tyr Glu Ser 3140 3145 3150 Ile Asn Ala Asp Glu Ala Val Lys Lys Tyr Glu Val Cys Asp Phe 3155 3160 3165 Tyr Thr Asp Gly Thr Phe Asp Ser Ala Gly Glu Lys Leu Cys Ser 3170 3175 3180 Glu Glu Lys Gln Asp His Leu Ile Thr Arg His Lys Ile Lys Glu 3185 3190 3195 Cys Ser His Glu Asn Glu Ser Glu Gln Lys Glu Lys Arg Leu Lys 3200 3205 3210 Val Gly His Glu His Glu Met Val Asp Gly Ile Ser Asn Met Gly 3215 3220 3225 Val Ala Asn Asn Asn Asp Asn Ile Tyr Gly Asp Asn Ile Tyr Gly 3230 3235 3240 Asp Asn Ile Tyr Gly Asp Asn Ile Tyr Gly Asp Asn Asp Asn Ile 3245 3250 3255 Tyr Gly Asp Asn Ile Tyr Asp Asn Asn Asn Asn Ser Cys Val Gly 3260 3265 3270 Gly Glu Asp Phe Ile Cys Asp Asn Lys Ser Asp Asp Ile Asn Phe 3275 3280 3285 Glu Phe Ile Lys Asp Leu Asn Lys Ile Asn Tyr Glu Glu Asn Lys 3290 3295 3300 Lys Glu Lys Lys Glu Met Leu Glu Ile Glu Lys Leu Asn Lys Glu 3305 3310 3315 Asn Ile Asn Gly Asp Lys Lys Asp Ile Tyr Lys Phe Lys Tyr Pro 3320 3325 3330 Cys Asn Ile Glu Glu Ile Lys Asp His Asn Ile Ser Met Lys Thr 3335 3340 3345 Ser Leu Ser Met Asp Val Tyr Glu Gly Ile Val Ser Thr Asn Lys 3350 3355 3360 Asn Ile Tyr Glu Ile Tyr Asn Glu Leu Lys Arg Lys Gln Tyr Phe 3365 3370 3375 Tyr Ser Val Cys Asp Ile Lys Ser Leu Arg Gln Ser Ile Ile Leu 3380 3385 3390 Leu Asp Gly Ile Ser Glu Ile Ser Trp Arg Ile Phe His Gln Glu 3395 3400 3405 His Thr Thr Phe Ser Ser Ile Leu His His Ser Glu Asn Tyr Lys 3410 3415 3420 Arg Leu Ile Asp Phe Asp Tyr Asn Thr Lys Ile Asp Asp Asn Asp 3425 3430 3435 Glu Asp Ile Asp Ile Lys Gly Ile Gly Val Asn Asp Leu Leu Lys 3440 3445 3450 Ile Tyr Arg Arg Leu Glu Ile Tyr Pro Asp Phe Ile Phe Asn Asn 3455 3460 3465 Asn Asn Val Gly Phe Ile Asn Arg His Asp Leu Lys Phe Ile Thr 3470 3475 3480 Lys Lys Asp Leu Leu Leu Met Asn Arg Lys Asp Tyr Glu Arg Asn 3485 3490 3495 Lys Lys Glu Arg Lys Lys Lys Asn Thr Asn Leu Tyr Pro Thr Asn 3500 3505 3510 Ser Gln Thr Asn Asn Asn Glu Asn Asp Asp Asn Asn Asn Asn Asn 3515 3520 3525 Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn 3530 3535 3540 Asn Asn Asn Asn Asn Asp Ser Arg Gly Asp Val Asn Lys Pro Lys 3545 3550 3555 Asn Asn Asn Ser Phe Asn Asn Asn Ser Glu Thr Lys Glu Gly Asn 3560 3565 3570 Lys Lys Lys Lys Lys Asn His Ser Asp Val Leu Asn Ile Lys Tyr 3575 3580 3585 Trp Arg Asn Ile Asn Thr Ala Val Asp Phe Phe Lys Ile Tyr Trp 3590 3595 3600 Ile Leu Tyr Asn Glu Ile Leu Ile Asn Ile Phe Tyr Lys Asn Ile 3605 3610 3615 Asn Tyr Ile Ser Cys Asn Asn Met Ile Glu Ile Leu Val Arg Asn 3620 3625 3630 Leu Phe Leu Tyr Lys Tyr Glu Leu Asn Ile Leu Lys Glu Glu Asn 3635 3640 3645 Met Cys Asn Leu Phe Asn Ile Gln Thr Trp Lys Asn Met Tyr Lys 3650 3655 3660 Ile Cys Phe Phe Tyr Val Tyr Pro Tyr Arg Phe Thr Phe Asn Lys 3665 3670 3675 Tyr Lys Lys Phe Phe Asn Lys Ile Lys Lys Ile His Phe Leu Asn 3680 3685 3690 Asn Leu Ser Phe Ile Pro Leu Ser Val Asp Val Leu Lys Leu Phe 3695 3700 3705 Met Asn Phe Phe Leu Asn Gln Tyr Leu Tyr Thr Pro Ser Leu Ile 3710 3715 3720 Lys His Phe Ile Ser Ile Ser Leu Ile Ile Ile Ser Phe Gln Ile 3725 3730 3735 Met Asn Glu Ile Phe Ile Lys Glu Ile Ser Lys Thr Tyr Val Arg 3740 3745 3750 Phe Ile Phe Lys Thr Ile Gln Asn Tyr Asn Lys Ile Lys Lys Tyr 3755 3760 3765 Ile Thr Arg Ile Ser Lys Tyr Met Tyr Phe Gln Lys Ile Tyr Thr 3770 3775 3780 Asn Tyr Ile Ile Ser His Tyr Glu Tyr Val Asp Tyr Ile Glu Arg 3785 3790 3795 Ala Asn Thr Phe Ile Gln Thr Glu Glu Thr Lys Lys Ile Arg His 3800 3805 3810 Asn Glu Ser Ser Ile Tyr His Asn Ile Glu Met Val Gly His Lys 3815 3820 3825 Gln Asn Glu Gln Lys Arg His Ser Leu Asn Ser Tyr Asp Arg Phe 3830 3835 3840 His Ile Phe Leu Phe Glu Lys Tyr Val Lys Asn Ser Ile Pro Gly 3845 3850 3855 Gly Glu Asn Ile Thr Leu Lys Tyr Val Asn Gly Arg Tyr Lys Leu 3860 3865 3870 Tyr Asp Asn Glu Gln Glu Val Val Arg Met Ser Asp Leu Glu His 3875 3880 3885 Phe Glu Lys Ile Lys Asn Val Tyr Ser Lys Lys Ala Thr Asp Ile 3890 3895 3900 Leu Lys Lys Lys Asn Glu Lys Met Lys Asn Asp Lys Glu Glu Thr 3905 3910 3915 Gly Asn Glu Gln Ala Asn Asn Cys Asn Lys Thr Leu Asp Gly Asn 3920 3925 3930 Lys Thr Leu Asp Gly Asn Lys Ile Leu Asp Gly Asn Lys Thr Leu 3935 3940 3945 Asp Gly Asn Lys Ile Leu Asp Gly Asn Lys Ile Leu Asp Asp Asn 3950 3955 3960 Lys Leu Leu Asp Asp Asn Lys Leu Leu Asp Gly Asn Lys Thr Leu 3965 3970 3975 Asp Gly Asn Lys Ile Leu Asp Asp Asn Lys Ile Leu Asp Ser Asn 3980 3985 3990 Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Gly Glu Asn Lys Ser 3995 4000 4005 Trp Asn Phe Gln Asn Val Ser Ser Tyr Asn Asp Leu Ile Lys Ser 4010 4015 4020 Val Leu Asp Lys Lys Asn Phe Phe Phe Ile Ile Asp Ile Val Asn 4025 4030 4035 Leu Phe Lys Asn Ile Gln Thr Leu Ser Tyr Leu Phe Asp Glu Glu 4040 4045 4050 Thr Ile Arg Tyr Val His Met Ile Ile Met Asn Ser Lys Ile Leu 4055 4060 4065 Ser Tyr Asp Leu Lys Glu Ile Glu Arg Lys Lys Asn Leu Gln Lys 4070 4075 4080 Lys Ile Tyr Pro Asn Phe Thr Ser Tyr Ile Thr Lys Gln Lys Lys 4085 4090 4095 Ala Asn Lys Leu Lys Cys Tyr Asn Tyr Asp Leu Leu His Ile Glu 4100 4105 4110 Lys Lys Trp Ile Leu Tyr Leu Ile Tyr Lys Thr Ile Leu Lys Tyr 4115 4120 4125 Tyr Asp Met Leu Ile Phe Lys Lys Lys Val Gln Lys Leu Met Leu 4130 4135 4140 Leu Met Cys Glu Ile Asn Asp Glu Tyr Ile Lys Tyr Ile Tyr Phe 4145 4150 4155 Asn Gly Tyr Ile Pro Ile Asn Ile Tyr Leu Ser Thr Asn Leu Ile 4160 4165 4170 Lys Tyr Asn Glu Phe Phe Thr Asn Tyr Tyr Ser Tyr Ile Tyr Asn 4175 4180 4185 Lys Asn Val Asn Asp Lys Trp Gly Asp Phe His Val Leu Tyr Phe 4190 4195 4200 Gln Asn Glu Glu Asn Asn Ile Lys Lys Glu Asn Lys Lys Asn Tyr 4205 4210 4215 Tyr Tyr Tyr Tyr Tyr Tyr Tyr Tyr Tyr Thr Leu Phe Lys Asn Lys 4220 4225 4230 His Val Leu Lys Arg Asp Tyr Asn His Ile Val Ser Asn Asn Ile 4235 4240 4245 Gln Pro Ile Asn Lys Met Asn Glu Pro Ile Thr Ser Asn Glu Cys 4250 4255 4260 Thr Ser Pro Asn Met Ser Tyr His Ile Glu Ser Asn Glu Ile Phe 4265 4270 4275 Lys Asn Glu Asp Asp His Asn Leu Asn Ile Tyr Asn Glu His Ile 4280 4285 4290 Met Tyr Asn Ser His Lys Asp Thr Asn Leu Leu Ser Ser Ser Asn 4295 4300 4305 His Met Gln His Val Gln Lys Asn Thr Leu Thr Met Asn Leu Asp 4310 4315 4320 His Ser Asn Asn Leu Ile Ile Asn Asp Asn His Lys Arg Asp Lys 4325 4330 4335 Glu His Asn Ile Asn Thr Lys His Trp Asn Lys Asn Ile Ile Asn 4340 4345 4350 Ser Asn Tyr Phe Asn Val Tyr Asn Asn Asp Ser Ser Pro Lys Glu 4355

4360 4365 Gln Ile His Leu Glu Glu Gly Gly Gly Gly Leu Ile Asp Val Asp 4370 4375 4380 Asp Asp Lys Lys Asp Asn Val Ile Asn Ser Asp Asn His Phe Lys 4385 4390 4395 Glu Thr Thr Tyr Asn His Ile Ala Tyr His Lys Asp Gln Glu Leu 4400 4405 4410 Ser Thr Glu Leu Asn Leu Phe Lys Tyr Lys Leu Tyr Asn Ile Ser 4415 4420 4425 Ser Glu Glu Ile Ser Asn Leu Tyr Leu Met Glu Asp Cys Asn Asn 4430 4435 4440 Glu Lys Gly Phe Phe Ser Cys Val His Lys Asn Glu Asp Phe Glu 4445 4450 4455 Tyr Lys Asn Ile Asn Ile Leu Asn Ile Leu Lys Tyr Gln Phe Glu 4460 4465 4470 Leu Val Pro Tyr Ile Asn His Leu Tyr Tyr Leu Tyr Asn Ile Tyr 4475 4480 4485 Pro Leu Leu Phe Lys Leu Lys Glu Ile Lys Asn Ile His Asp Met 4490 4495 4500 Lys Glu Lys Gln Ile Asn Leu Cys Thr Asp Gln Phe Leu Leu Ser 4505 4510 4515 Leu Val Asp Lys Asp Ile Tyr Asp Cys Tyr Asp Asp Val Asn Phe 4520 4525 4530 Ser Asp Ala Ser Phe Tyr Ser Asp Gln Thr Ile His Val Ser Glu 4535 4540 4545 Lys Glu Cys Glu His Lys Asn His Asn Ser Asn Lys Tyr Asp Cys 4550 4555 4560 Asn Asn Tyr His Pro Phe Asn Val Ser His Leu Ser Tyr Pro Tyr 4565 4570 4575 Lys Asp Gln Asn Gly Asp Glu Glu Asp Thr Leu Gln Ile Asn Ser 4580 4585 4590 Ser Leu Asn Ile Leu Glu Asn Val Val Asp Leu Asp Glu Leu Ile 4595 4600 4605 Lys Asp Leu Lys Lys Thr Lys Lys Ser Lys Lys Lys Thr Ser Lys 4610 4615 4620 Asn Lys Tyr Ala Asp Lys Phe Phe Val Ser Phe Phe Asn Thr Ala 4625 4630 4635 Leu Lys Arg Asp Glu Asp Lys Gly Lys Thr Asn Tyr Val His Tyr 4640 4645 4650 Arg Lys Leu Arg Asn Ala Lys Asn Asn Ile Lys Leu Lys Asn Leu 4655 4660 4665 Phe Leu Ser His Gln Gln Asn Asp Thr Ile Tyr Asp Asn Ile Asn 4670 4675 4680 Gly Asn Ile Cys Asp Asn Leu Tyr Gly Asn Val Asn Thr Tyr Gln 4685 4690 4695 Asn Ser Ala Ser Leu Ile Phe Val Asp Pro His Asn Leu Thr Asn 4700 4705 4710 Ile Asn Ile Ser Asn Glu Leu Asn Asn Asn Ile Asn Ile Asn Cys 4715 4720 4725 Ser Lys Asn Lys Lys Lys Lys Lys Pro Ser Ser Asn Asp Gln Asn 4730 4735 4740 Asn Thr Glu Asn Glu Asp Met Gln Asn Ser Ser Ser Asp Asn Pro 4745 4750 4755 Tyr Asp Asp Ser Asn Phe Ile Ser Tyr His Glu Ser Ile Asp Ser 4760 4765 4770 Thr Ser Ser Thr Asp Lys Tyr His Lys Arg Lys Met Thr Pro Met 4775 4780 4785 Glu Ser Met Phe Leu Lys Tyr Thr Leu Asn Thr Ser Glu Asp Ala 4790 4795 4800 Leu Ser Asn Lys Tyr Asn Val Thr Ser Lys Asn Lys Asn Lys Lys 4805 4810 4815 Glu Tyr Leu Ser Phe Leu Lys Tyr Lys Asp Ala Asn Lys Ile Gln 4820 4825 4830 Arg Tyr His Met Leu Leu Arg Lys Asn Gly Leu Gly Arg Leu Phe 4835 4840 4845 Phe Tyr Asn Leu Arg Lys Met Tyr Asn Tyr Val Tyr Asn Leu Tyr 4850 4855 4860 Asp Phe Ile Pro Leu Tyr Leu Ile Gln Asn Ile Leu Ile His Tyr 4865 4870 4875 Tyr Asp Ile Tyr Gln His Val Asn Gln Tyr Tyr Tyr His Asp Asp 4880 4885 4890 Lys Glu Tyr Pro Thr Phe Phe Thr Gln Gln Asn Glu Asp Asp Glu 4895 4900 4905 Tyr Glu Tyr Thr Lys Phe Ser Lys Asn Phe Phe Asn Thr Asn Lys 4910 4915 4920 Trp Lys Lys Met Tyr Ser Lys Tyr Glu Asp Lys Leu Asn Asn Glu 4925 4930 4935 Thr Tyr Pro Glu Pro Pro Lys Lys Tyr Ile Ser Asn Lys Gln Arg 4940 4945 4950 His Lys Glu Asn Ile Leu Ile Tyr Gln Asn Leu Gln Asn Asp His 4955 4960 4965 Met Asn Asn His Tyr Asn Ile Ala Asn Asn Asp Ile Glu Asn Lys 4970 4975 4980 Asn Gln Thr Asn Ser Ser Tyr Leu Phe Asn Val His His Gln Asn 4985 4990 4995 Cys Pro Ser Leu Ser Ser Asn Asn Leu Val Asn Ile Asn Ser Thr 5000 5005 5010 Leu Ile Asn Asp His His Phe Val Gln Thr Asn Asn Asp His Met 5015 5020 5025 Asn Ile Ile Gln His Met Glu Asn Ile Pro Glu Met Ser Glu Cys 5030 5035 5040 Asn Leu Lys Thr Asp Gln Asn Asn Asn Ile Phe Asn Phe Pro Gln 5045 5050 5055 Asp Lys Ile Asn Pro Asp Thr Met Lys Lys Asn Glu Lys Met Glu 5060 5065 5070 Phe Ser Val Asn Lys Lys Ile Lys Thr Asn His Asn Thr Thr Gly 5075 5080 5085 Tyr Asn Lys Arg Asn Asn Asp Arg Tyr Asn Tyr Ser Ser Thr Pro 5090 5095 5100 Asn Phe Phe Gly Lys Asp Glu Tyr Asp Asn Thr Ser Tyr Glu Asp 5105 5110 5115 Tyr Ile Lys Lys Lys Lys Lys Asn Phe Ser Ser Thr Ser Arg Arg 5120 5125 5130 Lys Arg Asn Asn Asn Ile Asn Met Lys Glu Phe Phe Ala Asn Gly 5135 5140 5145 Arg Tyr Asp Lys Leu Leu Ser Ile Lys Lys Ile Phe Met Ile Phe 5150 5155 5160 Tyr Asn Tyr Leu Arg Lys Cys His Phe Ile Asp Lys Lys Asn Ser 5165 5170 5175 Cys Lys Asn Leu Tyr Lys Ser Phe Leu Lys Lys Tyr Leu Lys Ser 5180 5185 5190 Ala Asn Val Tyr Leu Gln Phe Ile Ala Tyr Ile Ile Phe Ser Ile 5195 5200 5205 Tyr Asp Tyr Asn Ser Leu Ala Lys Lys Tyr Leu Asp Tyr Ile His 5210 5215 5220 Leu Tyr Ser Asp Ile Ser Lys Tyr Asn Ile Phe Leu His Ile Leu 5225 5230 5235 Asn Ile Asp Glu Asp Tyr Asp Lys Ile Tyr Ile Tyr Gln Thr Leu 5240 5245 5250 Gln Leu Ile Tyr His Pro Tyr Phe Tyr Leu Met Tyr Lys Leu Tyr 5255 5260 5265 Lys Tyr Leu Lys Ile Asp Ala Ser Pro Val Leu Leu His Asp Phe 5270 5275 5280 Tyr Glu Asn Trp Glu Glu Phe Thr Lys Ser Pro Ser Leu Phe Gln 5285 5290 5295 Pro Ser Lys Asn Tyr Phe Thr Leu Ile Lys Lys His Phe Lys Arg 5300 5305 5310 Lys Cys Val Ser Cys Asn Lys Ser Pro Lys Lys Ile Leu Ile Cys 5315 5320 5325 Leu Tyr Cys Gly Ser Thr Val Cys Leu His Glu Gly Asp His Val 5330 5335 5340 Ser Gly Pro Leu Ser Gln Thr Ile Ser Lys Cys Val Tyr His Thr 5345 5350 5355 Thr Ile Cys Gly Gly Glu Gln Cys Leu Tyr Leu Cys Leu Asn Thr 5360 5365 5370 Ser Ser Val Leu Phe Thr Ser Glu Asn Arg Phe Asp Phe Met Ser 5375 5380 5385 Gly Pro Tyr Val Asp Lys Asn Gly Asp Val Asp Tyr Gln Leu Lys 5390 5395 5400 Arg Gly Lys Asn Leu Tyr Leu Ser Ser Tyr Lys Leu Asn Lys Leu 5405 5410 5415 Phe Asp Val Ile Ile Asn Ser Ala Val Asp Val Glu Ile Tyr Lys 5420 5425 5430 His Thr Leu Lys Ser Glu 5435 1120805DNAPlasmodium falciparum 11atgataaagg cattatacaa aagtattgta aattttttat tgtcatcctt ttttgaaaac 60attgaagaaa aacaattaca aacatcatta atacgaggaa aagttcattt gcaaaatgta 120aaaataaaaa aatttttttg tgattttata tatttacctt atactatcaa atatggttat 180attcgttcct tggacttaca aataccactc ttgtatttgt ttcaaaaagc ttctttaaaa 240gtaagcgacg ttgtaatagt tttgaaggaa aaacatttca gcgaatataa catagaagaa 300gaaataaatg gaataaaagg agggaaaaag aataatttaa aacttgaaga ttataattat 360aatagtaatg atgtgaatca gaaaattaat aaaggcttaa attatttatt ttatgaatta 420tataataata tgtctattga tattgaaaat gtagaaatta tattagaaag ttctgaaaag 480actactaatg ttattggatt gtttgtaaaa aaaataagta gtgttaaaac tcataattgt 540acaaataaaa taataaaaga tttttctttt gtggatgcta ttttttatat gaatgatgaa 600aaagcgaaat atgcaattaa taaaattata aaaaaagaag aaaagaaaaa gaaaaataaa 660agaaatatta atgaagaaga tatatataat aaaattaaat atatttgtaa taatttacat 720aaaaaaaaat atataatttc agaaatacca tccattgttt taagtacaag tatgtatgtt 780agtaatagta ctgattatta ttatcataat tcaaaatttc tttttaaaaa aaatagctat 840tttaatatta catgtacaca ttgtgataaa tatctttttc aatgtaaaga agtgaaaaaa 900gataaatcga tatttcaaaa tatattcata aagaaagaaa aaattcaaca aagaaataaa 960cagctctata gtgaaattaa ttgtaaatgt atgaaaaata ataaaaatat tttccagaaa 1020gatcaaaata caaattgtac gtataattca gatcatgtac ctgagaactc tcttaagggt 1080actaatgaaa tgaataaaaa tattccccct gatcttgaca gtataaatgt gggacacccc 1140aagattgatg atgataatga aaaaaataac catgaaaata atagtcataa tgataatcat 1200aataatagtc ataatgataa tcataataat agtcataatg ataatcataa taatagtcat 1260aatgataatc ataatgataa tcataataat aatcataatg ataatcacaa tcttaaacat 1320aaacgtaatt ataatcatcg taatggtggt gtcggacaaa ataatactgg tgataacaac 1380cttttaaata atacatttaa taaacataac gaagaaagaa ccgtacaaga gaaagatgaa 1440aaaaaaagtg aagattgcat tgattgtaat tgtaaaaaaa atattagtga agatcttgat 1500aattgtttat attgtaggat taataataaa aatcaatcgt atattgaaaa atttcatatg 1560ttagaagaaa atataaattt aaaagaaaat gaatatatga cctttgaatt gaatgtagat 1620aaaatagatt taatattatc attcaaacaa ttctattata tcttagatat tgtaaattat 1680tattttatat atagttgtct tgttaatggt ttgtgtatag aagaatggtt aaatatccca 1740gaaattaatg atatattaat ttataaaaga gaattaataa aaaaaaagaa aaaagaaaaa 1800tttgataaag attttataga taaatttgaa tatacacata gctttcaaat tatttcaaat 1860ataaaaaaac atgtcgaaga atattcttta gacaataata aaaggaaaaa aagtataaga 1920ggaacaggcg aagatttatc aacacttctg aatagtaatc ataacaatga atataattat 1980aaaaatgaac ataatcgtca caatgaacat aatcatcaca atgaacatga taataataat 2040aataaggtga caaaatcaca atccgcgtcg tctcatttgt caaacgtatc aacatggaat 2100aaaccttttt ttgtatataa taaaaaaatt tcagtaaatt gttcatttaa cattttaatt 2160aaaaaattaa atttttgttt atgtagtggg gataaaataa ataaaatcat acaaattaat 2220acggatgatt ttaattttaa tgttaaatct tatattaatg ctgattttgc atattcctta 2280ttgttaaaag atattagtgt tttctttata cataaagata aaaaatatcc tttgtttgaa 2340ttatttaatg ttaatgaaca taataaaaaa gaagataaga aaaaagagaa tatgaaagta 2400aataaaagaa aaagtaaaac ttatatattc aatacacact tatgtaatga tgcgatgact 2460tcttttaaaa gcgtttcatc tataaaaaat gttccaggta atatgaataa tattacagat 2520aatatgaaaa atgataatta taataatata tataagaata atgaaaaaaa tgtggaaaat 2580ggaaagaaat gtagtaaata tgttgagtct agaaatttag tggaaataaa taatagacat 2640aatgaatatt caaaagaaaa gaaaaatata acaaatataa agaatgacaa tattaataat 2700aatgaaaata ataattatga taataatatt aataataaag aagaatatgc aatatcatta 2760ttaacattta ctaaatatga aactacacaa gctaaaacat ttataaaatt taataaaaat 2820attcttataa cattaagtgt taaagaaatt tataatattt taaatatgaa ctgtgattat 2880aaaccagtcg atttagaaaa aaaattgaaa tcttataaaa ttttatttta ttattatctt 2940ctaggaaaaa aatattgtaa agaattatat atggcatcag ataaaaattt tgaggatatg 3000aatatacatt ttatgcataa tatttatttt aatataataa ttaataaaaa atataattta 3060ttatttaaaa tgaaaaaggg acttgtaaat tctttcttct ttacaaaaat gtatttacat 3120tgtgatttga agaaactaac tatgataaca gaccataatg atatgcaaca aaatatggat 3180aacaaattag ttgatcaata tactgaacaa atgaaagaaa actcttcata tcagaatagt 3240caaagttgta gtaatacaag tagtgtgggc tttacaaatg aggacccaac gaaaagaaga 3300aataaagaaa aaaataatct aaatataaat ataaatttaa atttaagtac gaatgacgat 3360gtaataatta ataaaaatga agaaaaagaa aatgaaaata aagaaagtaa taggacagag 3420catataaaac gtgataattc tatacattcg aatgataatt gtagtatagg tactcatagt 3480gagcttttta aaaattcgga atataattct atgattaata ataataataa taataactct 3540tttataaata atgataatac aagtattgat aataaggtgt caatgcaatg tagaaagtat 3600tatcaggagt ttttaaaaat gaataattta gaagatatta aaacaattag aaaaatagaa 3660aaaagggcaa accaaaataa cacaagcaat aataatacta taaataatat acacaatata 3720aataatgtac acaatgtaaa taatatacac aatgtaaata atatacacaa tgtaaataat 3780atacacaatg taaataatat acacaatgta aataatatac acaatgtaaa taatgtacac 3840aatataaata ataattatta tgatcatatt tatagtcata acaatatcca tattaataat 3900catgcgatta tccacaataa cagccattat aataataaaa aaacaaacat tattctgaac 3960aacaacaaat atgatgaata taatgataaa cacagttttc atagcgaaaa tacaaattct 4020tatagtaatt caaataagga tgattattta aatgaagatt taaatttata taaagatcat 4080atgtatatac aaaattgtat aacggaaaaa gaagataata taattactac aatttatcag 4140aaaagaacaa attttgaact ttctcttttt attcttaaaa ataaaaataa tgaaaagata 4200caaaatatat tatctcataa aaataaaata aataagaaat atatattaaa accattaaaa 4260atatatattg atgaaattac atataatttt aaaaattcga gtgataagaa aataaccttt 4320tttcatacta ataattataa aaactatcta aattttacac ttgatgataa aattatattc 4380aaacttattt tcttttatta tcattataaa gattatgttg atcatatttt tgaaaagttt 4440gaaagcaaag atgaatccaa ctatttaaat ttaaatacaa tagatgtacg tagttataaa 4500actttacaaa ttttattaga taatgctgtt cataataaga atgatcactg gttcaatgaa 4560cagaaggatg ttcatgataa agatgagcag aataaaggaa caatatatta tgaacaaaat 4620gaacatgata tcaaaattga tgataataat gaggaggaga ataacgacca cacagataat 4680aataataaaa acaataataa taataataat aacaataata ataaaaacaa taataataat 4740aacaataata ataaaaacaa tatggtggat ggtcaaatcg aatgtaatga aaatgttaca 4800gaaaaagaga taccaagtaa tgtacccaat tcaaataact taacaaatga aagttcaaat 4860gataatcata ataaaacaac tattgaacat aataataatt tttgtgaaga acagaacaaa 4920aatgatttaa taaaaacatc ctttaatatt actcattgtg aagaagaaaa tgcaaataaa 4980aataaataca cggaggaaga taaaaaggaa ataaaaggaa atgataaaaa aaatgttgaa 5040atgtgtagaa aagatacatt agattatgta aaagaagata taaataaata taagatgtat 5100attaatgaaa ataataataa aaaagaagaa gaatattcac ataataataa taatcatcat 5160catcattacc atcataataa tatgttgaaa gatgatacat caaattataa ccatgtacaa 5220tatttccatg gagaaaaaaa agaaataata tgttttataa ataaaagtaa aatacatttt 5280aataattata aaaaaagtaa aagaatgata tcgatacatt tatcagatgt cctaatgtat 5340ttatttagtg ataataatat tacaaacttt ttatttaatt taaaaaaaat taatatatta 5400aattgtttat ctcatgaaga tagttataat agtttctttt catatataca acataaaaaa 5460ttttctacac ataaaataag taaaaaagga tcaaaattaa gtgataaaag aaagagttca 5520aatgttaatg gaatttattt ttataatagt gtttttaaat taattcaaaa cggtaccttt 5580cttttaaaat attcatcaaa gaataatatt tcatgcttat cttccataca tctaaaccca 5640aatcataatc attatataga aaataataat tcaaaagaaa aaaattattt tctaaacaac 5700aataataata ataataataa aatggaaata gaagaaacta ctgaatttag atattttaat 5760acaaataatt ttgaacaact aaataataca tcaggaaata aatctattga tggatccaac 5820gtgaaaatta ataatagtct tgattttgtt cataaaaatt attataataa tttgaataat 5880gactatttta attttaatgt acctcaggat tctaataaac gaaaggatgc cacaagttct 5940ccaagtaatg ttagtaataa aagtggggaa atcaaaagta gtcacaagaa ggaacaaagg 6000aaagataatc atatgaatga aaatattaga ggtagtcata gaagttatag tactcatcaa 6060agttatagaa cccatcaaag ttataaacgt catgctaata atagtaatat acgatgtagt 6120agcagcccaa gcgaaagaac acacatctcc aaaaaagatg aaataaaatt caataaagaa 6180aacattatga cattgttaaa tgattgtaca aataatgaag gagaaaatca tcatgttaaa 6240ataacgaaca aacaaatcaa aagaagtaaa agtaaagaac aatttgaaga tataataaat 6300agtaatacaa aaaaaacaaa tgatatagaa catatttggg atgaacaaaa taatcaaaac 6360aataaagaaa atgataatgt tgtaaataga gttgagaaaa aaagtattat tacggaaaat 6420tatatacctt ataaaagttg tatagaacaa attgatgata ttgattataa taaagaatcg 6480ttacaacatg aaaaaaacat agatggaaaa acaaatatta tatatcataa taataataat 6540aataatgatc atataaatat gaatactact acttgtattc ctataaatga aaagaacttt 6600gttatacaaa gaaataaaaa aatttctcat tactataatg caaaagaata taatgattca 6660aataaaagga gaagaaataa ttatatatta actggagagg taaataatat aaaaggttat 6720aataaagatg atatgcattc atcacatgtt aataatatga ataattcatt atgctcatca 6780gcttatcata atatgaaata tgaaaaatat gtagatattg agaaatatag aaatgatgac 6840gaattttatg aagctgaaga tgatgaagat gaagaaaatg atatagataa tcaagaagaa 6900gaatatattg atatgaaaaa aaaagaaaat gaaaatatta gtagtattga atttcatatg 6960attaatagtg aatttattgt agattattta tattttatat atttatttaa atggattaat 7020aaattatata gaaaatatga aaaaattaaa caattgtcag attttgcata ttcaaataat 7080aatagtacag atgatgaaga aagtaatata aatgtatcaa aatgtaatta tattcttttc 7140ttacaaaata ataaaattta tgtaccaata tataattatt caatatcaca atataagtat 7200tataatagtt taaagggaac ctcttatgta tccttattac ctgtattaga gttacatttc 7260tcatgttcct acaaatctat gaacgaaaac gatgaaaatg taaagtcgat aaacgtgcga 7320aatttgggaa gcgctcttgt cattccaagt attaacattt acaaacataa aaaaaacaag 7380aaaagcaata tatgtgtaat aacgtttaat aggaaggata agaataatga aagtatcatt 7440aacgatttaa gatttcaaat aaatatgaga agaaattata attatgaaat tatagaaaat 7500gaagatgata atagtagtag ttgtaataat atggagattc catattatga acctaaatat 7560acatcattaa atacatattg cacggttata tctaaagagc agatgtattc agaaaagaac 7620aagaataaaa ataataatat taacaacaat

aataatgata ataatataat tatgtatgat 7680aataatatgt ataattatca tggttaccat actaaaacag gatcatatcc aaaaaagatt 7740attagtttcc attgtgatcc tataatttta aatttttcaa aagatatatt aaaaaatgta 7800cttatatttt ataatacatt ttgttatttt ataaataatt gtagtaaaaa cgatgataca 7860ggtaatgata gtaatatgat tagagatatg ttatattatt tatacaacca aagtagtgat 7920atattatttg aatattttct tgatgatata cgtttaaatt gttatgatgg taatattaat 7980cgaaatgtac cattgatagt agttagtttg tttaatttaa aattcttttt acaaaaggat 8040ataataagta tatataattt tttgaatttt tatttaaata tagatatata tgatagttat 8100aaaatattat atgatacgtt attagaaaag atggaaatat atttaacttt tgaaaataat 8160agaaataaat tgaatatatt aaatttaaca ttagaaacat cttatattaa tattttatat 8220acaaataaaa gtaaaaatat attaataaat ttccttgagc caatattaaa taaaaatttt 8280tattatttca gttcatcata ttataataat ttggatatta caaaaaataa aagaagaaaa 8340cgaagtattt atcatcatca taatcataat tatcatcatg aatgttatga aaataatatt 8400ttaattaaaa atacaaaagg aaaatcatta gttacacata taacatctaa aatatttaat 8460tatacaggtt tatcaataaa tgtaagattt aaacaatcat tacgaaataa aatacaaaac 8520aatagtttaa ataataagaa taaaaataaa tatttatttt atgaattaca aaataatgag 8580catggggcat tattaaatga tgaaaatgga gaagtgtgtg aagtcgtagt tattataaaa 8640atatttaatt atatatttga aataatagat atagaaagaa ataaaaatac atgtgatatt 8700agatttttac ctataatata tgaattaaaa aaatttaatg aaaaaaaaaa aattactatt 8760aaaaagtatg atataaatta tgatatatat aatgaatatt cattaaaaaa taaagtattt 8820aaaacattta ttaaattata tatacaaaca aatattgatg aaaagggaat agtctcaata 8880tatttctcat caaatctatt tattaataat tcaacaaata gtgattttgt tttattgttt 8940caccctgtac tttattattt tatgttattt aatgaacagt ccacaaatag taacactaac 9000aataatataa acaacaacaa taaaaatgag gataacatgg tcgataataa tgaaaataat 9060gataaaaata atgataaaaa taatgataaa aataataatg aaaataagga ttgtgatagt 9120gtccataata ttaataatgt ggaaagcgat tcaccagtaa atataaactt tttcaaaaat 9180gataattata ataataatta ccataataat taccataata attaccataa taataatgac 9240aaaggtgcaa atataactat tattaattcc aatgaaaggt attttttacc tcttatttct 9300tttataccta ttattatttt atatgattat ttaaagaaaa aaaatgaaat cgaagaaaat 9360aattaccctg acaagtatcc aatgaattct aagaattctt atgtaacacc tgtctcaatg 9420gtatgtgatt taaaaggaag cttcagtgca gatataaaaa aaaataattt cataaaacga 9480tttaattcgt taaattataa tatgaatatt cataatggtg aagaggaaga aatatctaag 9540cagaatgaag ccaatgatat gttaaattat acccacgtgg ataatagtaa caacgaaaca 9600tcttgcagaa ataataatga agataaaata ttaagttata agaaaagaat tacttatgat 9660tattcttcga ttaattcttt aaaaaaattg tcatacaata acttaacacg tgctagctgt 9720tatgttaact ccagaagtag cgaaatgaac ctttgtaata taatatcgaa taaaggagat 9780aaaagaaatt ttatgaacaa tgaaaaaata atagaatctc ttaatttgaa tgctttaaga 9840gaaattaata aaataagaac taatagaata ttaaaaagat tagaaaaata tgtaaatgaa 9900aaaagtaata gtaagaatat gaaaaatatt aattatattt atattattaa aaaagaattt 9960tatgatttct atattcataa aaatgaaaat aataataaat gttattttga aatgtttaat 10020tttttaataa aaaatggagc caaaaaagtt ttaaataatt caagtataaa agaaattatc 10080tgtagacatt cctttgattt tttaaaatat aatttatatg cacaaacatt aaaattaaaa 10140actttaaaaa ttatatgtta tgataagtat agtagatatt taagggagaa gaaaaaggaa 10200atggaagaag ctaaaacgta taataataat aataataaaa ataacatcaa caataataat 10260aacaacaatg ataataacaa caataataat aacaacaaca ataataataa caataataat 10320aataatatga agaaaaagca aattggtgat atatatatga aaaattataa agaagaaaaa 10380aatgatataa aggaagaaca agaaaaacat gataatagta gtagtattgt ggtgttaaat 10440aaatttcatg attattatga taaaagagat aagtattatt ttgaaaacaa ccacaggaat 10500gaccattaca caagtgactt aaataatgat gataataata aatataatat ttatgataat 10560aataaatata gtatttatga taataataaa tatcatattt atgataataa tgaaagtaac 10620aataaaaagg atgattttta ttatagcata cctactaatc tcgatatttc acttaaatgt 10680ataaatcgaa atgaaagaca catcaacagt cttctattta atcatagtaa gaggaaatct 10740acattaagca acatactaat aaaacatgat atgattaaaa atagtgtatc taaacatatc 10800ctgaataatg attatgcata tgaatatgat aaaaaggaag aaatagaata taactataat 10860aataaggata attgtgaaaa aaatatatat gtgcttgatg taaaaaataa aaaacaaaat 10920aataataata atatgaacga tgatgatgat gataataata atatgaacga tgatgatgat 10980gataataata atatgaacga tgatgatgat gataataata atatgaacga tgatgataat 11040aataatgata ataataatga taagaagaag catcatatta tccattataa taataataat 11100attataaaaa agaaaagaag gaaatttata tttactgcaa agaaacaaag tagttatagt 11160aatacaatta tatgtgaaca aaagaatata gatgtgaata aaatatattc gtttggtata 11220tatattgatt atatatttga aatccataac tttatgcatg aagaaatgaa aattggttat 11280tgtcagaaat attatataaa cagtatacct aaaaagatta aaagaattaa acgagaagag 11340atatcaccta aatcaataaa atatttagat acgataccga aatatattaa attattttat 11400gtattaaata atgataatta tatatttaat tttttttcta aaaaaattca attatataaa 11460tatgctaata caccagataa aattcatcaa ataaaaatgt atatagaata tattaatgat 11520gaaaataaaa atataatata taagaaaaga aaatataaat taaattcttt atttaaaaga 11580aaatcaaaaa ataaaaggaa atataaatat ctagaatcaa aagattatga taaattatgt 11640aaagaagtac ctaagttttt ttatcttaat attacatttc gaaagaaaac agatcattca 11700aataatacat attataatga aaataatttt aaacatgtaa tagacagtaa tgttttgcaa 11760ttattaattt atccatcttt cttttttcaa aataatctag ataacgattt aataattaaa 11820tccaatggaa atttacaaaa aattaaaaaa aaatcaataa tgttttttgg tgatgttgat 11880aatagtaaag ttatattaca aatagtatat aataatcaaa tattcctttc taaaaaaata 11940aaaataaatc aagtatgttt aaataaaaaa atcatcttaa caagtaaaaa taaaaaagaa 12000aatattttct tatctatgaa tattacatta aatggtaata tctataatac aaaaacaatt 12060actattaata atagatatac aattattaat aataccaatc gaactttata tgtgcaagaa 12120gttaataaag atttcaaaat tattaaaaaa aaggaaaaaa aatatatgac gtattcattt 12180tctttagaaa atttacaaaa tcaaaataat cattcaaatg atgaaaatat ggaaaaatta 12240tatataccta gtagtaatga tcttactaat ataaaacgta attataatca aaaagaaaaa 12300aatgaaaaaa atgaaaaaaa agaaaaaaat gaaaatttaa acaaaattac aatcaataaa 12360cgatcagatt taacgtatga gcacgaaaat gatgaaacaa aaattatgga tacttgtgaa 12420atttctgata agaaatatac ggaaaaaggt atgacaaaaa tatgtgaatt taataaaaag 12480catataaaat ataataaaca taaaaaatat aacgacaaga aatataatca aaaggaaaac 12540aataataata ataataatac tacgagtagt aataatagta atagtaatag taatagtagt 12600aacaacagac ctaatattat ttcaataaaa ccatatgaat ctttttatta tcatcctatt 12660aacacaaata aatgcttcct aactttttct tatacaaata ttatggaatg taaaaatgat 12720aagaattttt atagaagaag aggtagctat gaatttacac atacacaaaa tgaaatcaca 12780aacttattca ataattattt aaaaaaaaaa attatgaaat ctcaaaatat atctataata 12840aatgataaag aagaaaacat aaataataat gtacataaaa atcaagaatt ctataattta 12900ttttatacta gtcctataga aatcgaaaag gtaggtaatt acaaaatttt tcacaagaca 12960gtacataaaa ttgatgttac gtacaataat gaaaaagacg caaccgacga tcaacctaca 13020tatgaggatg atattattca aaaggaaata aaacaacaag aaaaagaaat tgaagaagaa 13080ataaaaagag aaatagaaga actagaagaa catcgtgaaa aggaaactat tattgtattt 13140tcaaatttga aagattcata tgaaaaagaa atgcaaaaaa aaaaaaaaga tcgacataag 13200aaaaataact ttcttaatat aaactctgat catattaatg ataagaacat tattaattct 13260tataaaaatg aacaaaccac tgaaatgata tatatacaac acgactatac agataatata 13320cctgctgacg atgagaaaaa aagttcgcat aatgaaacta tacttataga tgatttaaca 13380ttatttaata aaaataataa acatagtatt aatgaaaggg aaaataaaga aaacatattt 13440aatgaaacgg ttttgacagt cgataataat gatactgaac atataaaaag cgactatgta 13500aatataaaag gcgattgtat aaatataaaa ggcgattgta taaatataaa aggcgattat 13560gtaaatataa aagataattg ggtagatata aaagataatt gtgtaaatat aaaagataat 13620tgtgtaaata taaaagataa ttgtgtaaat ataaaaggca attgtgtaaa tacaaaggag 13680gattctcata ataattatca acagaatggt atgaatctaa gacatgattc gttttgtgaa 13740aattatgtta tagattttaa tgatgatgat tcttgtattc attttaaaaa aaataaaaat 13800gatataaata cagcagatga tataaataat aatacggtga ttatccctat taaaagtata 13860aagcaactag aaggagaaga aaatgtgcat aatatagaaa ataaaaataa taacagcaat 13920aataataata ataatctttt atgtgaattg aataaaacga gtgataataa agaacgaata 13980aacacaaaac atatgttttt atataaagaa gaaagtatta ttacagaaat taatatagca 14040ttaaatagta atacaaatat cgaaataaat atatctatag aaaataaccc agatactatt 14100atatataata aaacaaacta ttatttaata ttttatcaaa gaaaaacaaa taaaaaaata 14160gtaaatttgt tgaaaccttt tgataaagaa atattcggat ggactgatgt ttgtaaacct 14220aaagttataa aatgcttttt aattattcaa aaaaatgatg tgtatgtatt ttcatgtaat 14280ttaaatatag taaaagaaca taataatatt atattaaata ataatagaag aattattgtg 14340ttaaccaaaa ttgaaaatgg gaaacgagtt ttttgtttag aagaaatgaa tatcgaatta 14400ttagattgta atgaaaaata tgaacacaaa aaatctctag ctccattttt ttccgcaagt 14460aaaggtttta atagatttag agaaaagaaa aaaaagaaaa tcaaaaagaa attacataaa 14520ataagtttat ataatagttt aatcaaatca tcacaaatga gaaaggaaaa gaataaaagt 14580aaaaataaaa aaaaaaataa aaaacaaaaa aaaaatcaaa tgaatcaaat gaatcaaacc 14640aatcaaacca atcatatgtt acaaaaaaac aaacaaacta atgataacta tttagtaaaa 14700tataaaaatg aaaaatttaa taagaaatta ccattactta gtaaaaaacg ttttaatatt 14760gcatatttaa aaaaaaaaag aaaacaaatc aaaaaaaaaa aaaataataa atattttcat 14820ttaagttcct ttttcctatt aaaaagtatg gattcatcaa tccataaaga tgttgaaaag 14880gaaaggaaaa aacaaaaagg aaaaagccaa caggcagaaa aattattcaa ggtagaaaaa 14940attgtacaca atgaatctta caatgaatcg tataatgaat cgtataatga atatcataat 15000gaatatcaca atgaatatca caatgaatat cacaatgaat atcataacga aaataatggt 15060aatataaatt caattaagta taataatatt attaaagaag aagaaaatgt gaaaaataaa 15120aatgcaacaa gtaataataa tagtagtttt agtaaaaaaa atattaaatt aaataatgta 15180tctataaaaa aagatgataa aaaaaaatct acatcaaaat ataaaagctt gaattctaaa 15240aaacatatta ataaattttt taatgacaat tatacatttc gaaaaaaaaa gaaaagaaaa 15300aaaagtaata aaaaaaattt atatgtgaat aaaaataatg aagagacaga aacaagtgat 15360tattatatag aacataatga aaacaagaaa aatatcgaac tttcttctca tgataacaat 15420tcttttgata atgagaaaag gaaaactagt ttaggcatga caaaatccaa aggatataac 15480gatcttttta ctgttaagtt tagaaaaagt gtaaatgaaa atgataaaat tgatgaatat 15540aaagaaaata gttttgaaga ctctagtaca tataatgatt catttttaag cagttcttca 15600aataatgaga attatatttc taataataat aagaaacaca tgtttcttaa tagatattat 15660gataataaag atgataagaa gaaatataga gaatataatt taatatataa taaagaaatg 15720aaagctaaaa ataatttaaa aaaacctaag aagaaaatta aaagaaaact tagtgatttt 15780atatttacaa aaattaaaaa aaatcaaaag aaatttaaag gacttagtag aatatatcca 15840atgtttttaa aaagtaataa aagtgatgaa tttaatgttc ttataagaaa ttatttatcg 15900gacaaagatg atagcactag tataaggaaa gaatataaaa ttataaatga taattatata 15960aataataatg aaaataataa taatgaaaat atgaattatg atttaaaaac aaatacttat 16020aataatattc ttcataataa taattataga ttagataaaa aaagtgtatt tgaagaaaat 16080agaaaaatac ccataaaatt tataacaaat ttaaatgtaa tgttacgtgg aataaaaatt 16140agtttatttg agcacacctg tgattatgtt atatcttttg aaatcaatga attattagtt 16200acaaaggaat atatggaata tatagattat tataatgtag acataaaaat aaataatatt 16260ttatgttatg ctgtatataa atatttaagt gcatgtgctg tattatatac aaataatgat 16320agcaacataa aaaataatca cttacgtatg ttaatgaatg aattaaaaat taaaaggaat 16380ttatttttac aaaaaaaaag aagaaatgaa aataaaaagg aatattctga tgttaatatt 16440gacaaaaata atgacaatat taaaaataat gacaatatta aaaataatga ccatattaat 16500gataatgacc atattaatga taatgaccat attaatgata aggaccatat taataataag 16560gaccatatta ataataagga ccatattaat gataatgaaa aagaaatgta ctcatcaggt 16620tcatccagaa caagttatac aaataaaaag aagggatata aatataaaaa caattataag 16680aaaaaaaaaa aaaaaactga aaaaaatatt tatgataata aaaaagccaa aaaaaaaaaa 16740aaagagaaag tagagaaaaa gaaaaagaac ctttttgtaa acaatgaatt tcttatatgt 16800aattttcaat atgtaaggaa atgtgaaaca ttatttttta aatatttctt attaagttta 16860aaacctattg ttattaatgc agatattaat tctataacta tattgttcta tttttataaa 16920acgttttata attttcatac caaaaaaaat gaacagggtc aaggagtcca aaatggattc 16980aacacggata attatgaaaa ggtgcacatg agggaaataa atatgatgaa agaacaacaa 17040caacaacaaa aaaaaaaaaa aaaagaagaa gaagaagaag aagaagaaga agaagaagaa 17100gaagaagaaa aaaaaaaaaa aaacaaggaa aatgtattac aaaaaaaaaa aaccaaacaa 17160gaaatggaga gacctttaaa agattattat tattattatt attcatctat gaatatatta 17220aatgatccag ataataataa aagatgtgat atacaaaaaa accgtatgat cgaaaaaaat 17280gatagctttt ttataaatgt agatgaaaat agaaaacaaa aaatacaaga tataactggt 17340gatcatatta ttgataataa tattttaagt cgaatgaaaa atatggaatc atttattata 17400catgaagaac aacaaaaaaa tgatttaact ttaacattac ataataatat atataatgta 17460gaaggagaat attataatat aaaaaagagt aatatgaatt attataataa tataattaac 17520aataaaaagt cttctgttca aataaaaaaa aaaacaactc ttatcgaaaa aaatgatatt 17580aataataaat atgtatattt acaatatata agtatagata aaataaatat tttattaaat 17640ttccaatcag aatataaaaa agaattaaca aatcatgtta atgatttatt ttataaagaa 17700catataagtg cttataataa attaggagat atttctaatt gtaatatata tttaaaaagt 17760ttatccaata tacatatctt tacaaattat aatttgttaa taaatttcct acaaaatttt 17820tattataacc aaactatagt taacctatca aacttattaa tttcttttaa tattattgga 17880agccctacat ctttaatagc gcatgttaaa aatgcattta atgaattctt ctatataatt 17940aatgaagaca cactatccaa aaaaaacaga tatgatgatg acaaggacat taacgattac 18000acaacaaaat acaacagaaa aaacaacaaa aaaaacaaca gaaaaaacaa caaaaaaaac 18060aacagaaaaa acaacagaaa aaacaacaaa aataatgata ataacaatat taatagtatg 18120tatggtagtc attataataa taattatatt tattataacg attattatta taataataat 18180tacatgtatc gtcgtagtac tcttaattat cattattatg aacaaggtta tgataaatac 18240aaattgaatc gtatatataa taataacccg aaattaagaa aaaataataa agaaagaaaa 18300aataatatac aacaaattaa ttatacaacc aaaaaagaag acaaacataa tttcctaaat 18360ttaatagatc aatccaggga aagctcattt ttagatgaga aagatgattc cttaaccaat 18420gatgataaat atattatgca tctactaaat gattataatt cttcttatca aaataattta 18480ttaagtgaag aagaatcttt tgttaaaagg aatttcagct ggaacttttt taaaaaacaa 18540catacaaata ataataataa taattcggat gatgacaatt attttaatga ttcatataat 18600aacataaaac atgaaagttc ggggaactta aattatatgt acacaaaaag tattaaaaat 18660aaagatttac ataaactagt aattgatgat aataactctt ctatgaattc attccttttt 18720aataataaaa aaaatgttat catattagat caagtttcta aaaaaaagaa aaagaaatta 18780caatatatca aaaattttga agaaccacaa aagggacata tatatatgga tgatgacatg 18840tatatagatg tagatgtaga tctaaattct tatgcggaag gaaaaaatac tacaaaagat 18900acatccaaat atccaccaca ttttaaaata tcatatgatg aagaaaaaaa aaaaaggaaa 18960aagaaaaggc tttcaaccgg tagtaataat aatagataca aaaatgatgc ggaaatatta 19020gatatgaatc caaacaaaaa aaacaagaaa aaaaataaaa tgctaaaaaa taataataat 19080aataataata ataataaaaa taaaaaaaat aaaagaaata ataaaagaag tggttgtact 19140aaaatgttca gattttttaa aaaaatagga ttaaatatta tttatatttt ttcaaaaact 19200aagagaatat gtattggtat atatgtatta tttatacaat taatattatc cttattattt 19260tctattacac taatattaca atctttaata aatttagtag aaaaggtaaa tataaataat 19320gcagataatg tgtttttaat atttaataaa aaatatgaaa gaaaaaaagt agtggtattt 19380aataatatag aggaatataa taaatttaat attaccaaat tattttatta ttatattagt 19440atatatatga atatagtgaa tttcttatat tataatatgt tatataaaat gaagaagaaa 19500aaaaaagatt tatcgggaga agataaaatt tttatgaaga aattaaaaat caatttaaaa 19560aataaaagaa aattttcttt ctttattaat ttctttttaa atatatttaa tatcatcaat 19620atattattat ttggacatat agttttctta cttttatgtt tattaacaat gaataatatt 19680tttcaaaaaa tacttacagc atgtattaaa ttagtatatt accaaagaaa taaaaattat 19740ctagattttc tcaataaaaa cagttttaat catatgaata ttataagtta tattaaaatt 19800aatcaagtta taagtaaata tgtactatta aaagaaccct tcaaatattt cttatctcat 19860caagatttct tggatattaa agcacctaaa aaaagaaatt attttgtata taccaaagag 19920ctcttctttt atatacaaga aaataaaatt gtattcttat ttagaaaaaa cgatatgaaa 19980aaaattgaca ttattataca tactataaat aatagtttcg caataaatag ttactataag 20040aaaaagggta gtaagacagc caaaaaaagt agatccaaaa ataccccatc gaattatact 20100cttaatatgg aacatgtgac aggagaagaa aataatagct ttacttcatc tttatataac 20160aaaactaacg tatcgaatga tatatcagag gataaaaatg ttatatgttt tttaaatcaa 20220gaattagctt taaccaaaag aaacgaaatt atacatataa ataataaaaa ctatgattat 20280attatttcca taaaaataac aatcaaaaat aatatgaatg ttatggagaa tatttattat 20340aaccccaaaa aattttttaa gtgcttgtca aaaatacaaa aaagcttcaa taatatttat 20400ttacctttat gtaaaaataa taataatgaa accaaaaata ataaggaaaa acatttaaat 20460aataaaggtg cagatatata taaagcagat ataaatgtaa aaaaaagaag aacttttttt 20520tcagcaatta tttgtttctt taaaagcatt ttcatatttt tgtacttttg tctatgccca 20580tggaaattgt taaagttaaa aaagaaaaaa tcaaatgaca ataaaaaaaa acaaatggac 20640gaaaaagata aagaaaatgc agtccataaa aatatacaac taaaacataa aaataaaaag 20700gaaaagacaa cacccaacag tattattttt aaattaaact ttgaagattt tcaatcaact 20760ttaaatatat ttgaatgttt gtgtaggatt atgaataata attaa 20805126934PRTPlasmodium falciparum 12Met Ile Lys Ala Leu Tyr Lys Ser Ile Val Asn Phe Leu Leu Ser Ser 1 5 10 15 Phe Phe Glu Asn Ile Glu Glu Lys Gln Leu Gln Thr Ser Leu Ile Arg 20 25 30 Gly Lys Val His Leu Gln Asn Val Lys Ile Lys Lys Phe Phe Cys Asp 35 40 45 Phe Ile Tyr Leu Pro Tyr Thr Ile Lys Tyr Gly Tyr Ile Arg Ser Leu 50 55 60 Asp Leu Gln Ile Pro Leu Leu Tyr Leu Phe Gln Lys Ala Ser Leu Lys 65 70 75 80 Val Ser Asp Val Val Ile Val Leu Lys Glu Lys His Phe Ser Glu Tyr 85 90 95 Asn Ile Glu Glu Glu Ile Asn Gly Ile Lys Gly Gly Lys Lys Asn Asn 100 105 110 Leu Lys Leu Glu Asp Tyr Asn Tyr Asn Ser Asn Asp Val Asn Gln Lys 115 120 125 Ile Asn Lys Gly Leu Asn Tyr Leu Phe Tyr Glu Leu Tyr Asn Asn Met 130 135 140 Ser Ile Asp Ile Glu Asn Val Glu Ile Ile Leu Glu Ser Ser Glu Lys 145 150 155 160 Thr Thr Asn Val Ile Gly Leu Phe Val Lys Lys Ile Ser Ser Val Lys 165 170 175 Thr His Asn Cys Thr Asn Lys Ile Ile Lys Asp Phe Ser Phe Val Asp 180 185 190 Ala Ile Phe Tyr Met Asn Asp Glu Lys Ala Lys Tyr Ala Ile Asn Lys 195 200 205 Ile Ile Lys Lys Glu Glu Lys Lys Lys Lys Asn Lys Arg Asn Ile Asn 210 215 220 Glu Glu Asp Ile Tyr Asn Lys Ile Lys Tyr Ile Cys Asn Asn Leu His 225 230 235 240 Lys Lys Lys Tyr Ile Ile Ser Glu Ile Pro Ser Ile Val Leu Ser Thr 245 250 255 Ser Met Tyr Val Ser Asn Ser Thr Asp Tyr Tyr Tyr His Asn Ser Lys 260 265 270 Phe Leu Phe Lys Lys Asn Ser Tyr Phe Asn Ile Thr Cys Thr His Cys 275 280

285 Asp Lys Tyr Leu Phe Gln Cys Lys Glu Val Lys Lys Asp Lys Ser Ile 290 295 300 Phe Gln Asn Ile Phe Ile Lys Lys Glu Lys Ile Gln Gln Arg Asn Lys 305 310 315 320 Gln Leu Tyr Ser Glu Ile Asn Cys Lys Cys Met Lys Asn Asn Lys Asn 325 330 335 Ile Phe Gln Lys Asp Gln Asn Thr Asn Cys Thr Tyr Asn Ser Asp His 340 345 350 Val Pro Glu Asn Ser Leu Lys Gly Thr Asn Glu Met Asn Lys Asn Ile 355 360 365 Pro Pro Asp Leu Asp Ser Ile Asn Val Gly His Pro Lys Ile Asp Asp 370 375 380 Asp Asn Glu Lys Asn Asn His Glu Asn Asn Ser His Asn Asp Asn His 385 390 395 400 Asn Asn Ser His Asn Asp Asn His Asn Asn Ser His Asn Asp Asn His 405 410 415 Asn Asn Ser His Asn Asp Asn His Asn Asp Asn His Asn Asn Asn His 420 425 430 Asn Asp Asn His Asn Leu Lys His Lys Arg Asn Tyr Asn His Arg Asn 435 440 445 Gly Gly Val Gly Gln Asn Asn Thr Gly Asp Asn Asn Leu Leu Asn Asn 450 455 460 Thr Phe Asn Lys His Asn Glu Glu Arg Thr Val Gln Glu Lys Asp Glu 465 470 475 480 Lys Lys Ser Glu Asp Cys Ile Asp Cys Asn Cys Lys Lys Asn Ile Ser 485 490 495 Glu Asp Leu Asp Asn Cys Leu Tyr Cys Arg Ile Asn Asn Lys Asn Gln 500 505 510 Ser Tyr Ile Glu Lys Phe His Met Leu Glu Glu Asn Ile Asn Leu Lys 515 520 525 Glu Asn Glu Tyr Met Thr Phe Glu Leu Asn Val Asp Lys Ile Asp Leu 530 535 540 Ile Leu Ser Phe Lys Gln Phe Tyr Tyr Ile Leu Asp Ile Val Asn Tyr 545 550 555 560 Tyr Phe Ile Tyr Ser Cys Leu Val Asn Gly Leu Cys Ile Glu Glu Trp 565 570 575 Leu Asn Ile Pro Glu Ile Asn Asp Ile Leu Ile Tyr Lys Arg Glu Leu 580 585 590 Ile Lys Lys Lys Lys Lys Glu Lys Phe Asp Lys Asp Phe Ile Asp Lys 595 600 605 Phe Glu Tyr Thr His Ser Phe Gln Ile Ile Ser Asn Ile Lys Lys His 610 615 620 Val Glu Glu Tyr Ser Leu Asp Asn Asn Lys Arg Lys Lys Ser Ile Arg 625 630 635 640 Gly Thr Gly Glu Asp Leu Ser Thr Leu Leu Asn Ser Asn His Asn Asn 645 650 655 Glu Tyr Asn Tyr Lys Asn Glu His Asn Arg His Asn Glu His Asn His 660 665 670 His Asn Glu His Asp Asn Asn Asn Asn Lys Val Thr Lys Ser Gln Ser 675 680 685 Ala Ser Ser His Leu Ser Asn Val Ser Thr Trp Asn Lys Pro Phe Phe 690 695 700 Val Tyr Asn Lys Lys Ile Ser Val Asn Cys Ser Phe Asn Ile Leu Ile 705 710 715 720 Lys Lys Leu Asn Phe Cys Leu Cys Ser Gly Asp Lys Ile Asn Lys Ile 725 730 735 Ile Gln Ile Asn Thr Asp Asp Phe Asn Phe Asn Val Lys Ser Tyr Ile 740 745 750 Asn Ala Asp Phe Ala Tyr Ser Leu Leu Leu Lys Asp Ile Ser Val Phe 755 760 765 Phe Ile His Lys Asp Lys Lys Tyr Pro Leu Phe Glu Leu Phe Asn Val 770 775 780 Asn Glu His Asn Lys Lys Glu Asp Lys Lys Lys Glu Asn Met Lys Val 785 790 795 800 Asn Lys Arg Lys Ser Lys Thr Tyr Ile Phe Asn Thr His Leu Cys Asn 805 810 815 Asp Ala Met Thr Ser Phe Lys Ser Val Ser Ser Ile Lys Asn Val Pro 820 825 830 Gly Asn Met Asn Asn Ile Thr Asp Asn Met Lys Asn Asp Asn Tyr Asn 835 840 845 Asn Ile Tyr Lys Asn Asn Glu Lys Asn Val Glu Asn Gly Lys Lys Cys 850 855 860 Ser Lys Tyr Val Glu Ser Arg Asn Leu Val Glu Ile Asn Asn Arg His 865 870 875 880 Asn Glu Tyr Ser Lys Glu Lys Lys Asn Ile Thr Asn Ile Lys Asn Asp 885 890 895 Asn Ile Asn Asn Asn Glu Asn Asn Asn Tyr Asp Asn Asn Ile Asn Asn 900 905 910 Lys Glu Glu Tyr Ala Ile Ser Leu Leu Thr Phe Thr Lys Tyr Glu Thr 915 920 925 Thr Gln Ala Lys Thr Phe Ile Lys Phe Asn Lys Asn Ile Leu Ile Thr 930 935 940 Leu Ser Val Lys Glu Ile Tyr Asn Ile Leu Asn Met Asn Cys Asp Tyr 945 950 955 960 Lys Pro Val Asp Leu Glu Lys Lys Leu Lys Ser Tyr Lys Ile Leu Phe 965 970 975 Tyr Tyr Tyr Leu Leu Gly Lys Lys Tyr Cys Lys Glu Leu Tyr Met Ala 980 985 990 Ser Asp Lys Asn Phe Glu Asp Met Asn Ile His Phe Met His Asn Ile 995 1000 1005 Tyr Phe Asn Ile Ile Ile Asn Lys Lys Tyr Asn Leu Leu Phe Lys 1010 1015 1020 Met Lys Lys Gly Leu Val Asn Ser Phe Phe Phe Thr Lys Met Tyr 1025 1030 1035 Leu His Cys Asp Leu Lys Lys Leu Thr Met Ile Thr Asp His Asn 1040 1045 1050 Asp Met Gln Gln Asn Met Asp Asn Lys Leu Val Asp Gln Tyr Thr 1055 1060 1065 Glu Gln Met Lys Glu Asn Ser Ser Tyr Gln Asn Ser Gln Ser Cys 1070 1075 1080 Ser Asn Thr Ser Ser Val Gly Phe Thr Asn Glu Asp Pro Thr Lys 1085 1090 1095 Arg Arg Asn Lys Glu Lys Asn Asn Leu Asn Ile Asn Ile Asn Leu 1100 1105 1110 Asn Leu Ser Thr Asn Asp Asp Val Ile Ile Asn Lys Asn Glu Glu 1115 1120 1125 Lys Glu Asn Glu Asn Lys Glu Ser Asn Arg Thr Glu His Ile Lys 1130 1135 1140 Arg Asp Asn Ser Ile His Ser Asn Asp Asn Cys Ser Ile Gly Thr 1145 1150 1155 His Ser Glu Leu Phe Lys Asn Ser Glu Tyr Asn Ser Met Ile Asn 1160 1165 1170 Asn Asn Asn Asn Asn Asn Ser Phe Ile Asn Asn Asp Asn Thr Ser 1175 1180 1185 Ile Asp Asn Lys Val Ser Met Gln Cys Arg Lys Tyr Tyr Gln Glu 1190 1195 1200 Phe Leu Lys Met Asn Asn Leu Glu Asp Ile Lys Thr Ile Arg Lys 1205 1210 1215 Ile Glu Lys Arg Ala Asn Gln Asn Asn Thr Ser Asn Asn Asn Thr 1220 1225 1230 Ile Asn Asn Ile His Asn Ile Asn Asn Val His Asn Val Asn Asn 1235 1240 1245 Ile His Asn Val Asn Asn Ile His Asn Val Asn Asn Ile His Asn 1250 1255 1260 Val Asn Asn Ile His Asn Val Asn Asn Ile His Asn Val Asn Asn 1265 1270 1275 Val His Asn Ile Asn Asn Asn Tyr Tyr Asp His Ile Tyr Ser His 1280 1285 1290 Asn Asn Ile His Ile Asn Asn His Ala Ile Ile His Asn Asn Ser 1295 1300 1305 His Tyr Asn Asn Lys Lys Thr Asn Ile Ile Leu Asn Asn Asn Lys 1310 1315 1320 Tyr Asp Glu Tyr Asn Asp Lys His Ser Phe His Ser Glu Asn Thr 1325 1330 1335 Asn Ser Tyr Ser Asn Ser Asn Lys Asp Asp Tyr Leu Asn Glu Asp 1340 1345 1350 Leu Asn Leu Tyr Lys Asp His Met Tyr Ile Gln Asn Cys Ile Thr 1355 1360 1365 Glu Lys Glu Asp Asn Ile Ile Thr Thr Ile Tyr Gln Lys Arg Thr 1370 1375 1380 Asn Phe Glu Leu Ser Leu Phe Ile Leu Lys Asn Lys Asn Asn Glu 1385 1390 1395 Lys Ile Gln Asn Ile Leu Ser His Lys Asn Lys Ile Asn Lys Lys 1400 1405 1410 Tyr Ile Leu Lys Pro Leu Lys Ile Tyr Ile Asp Glu Ile Thr Tyr 1415 1420 1425 Asn Phe Lys Asn Ser Ser Asp Lys Lys Ile Thr Phe Phe His Thr 1430 1435 1440 Asn Asn Tyr Lys Asn Tyr Leu Asn Phe Thr Leu Asp Asp Lys Ile 1445 1450 1455 Ile Phe Lys Leu Ile Phe Phe Tyr Tyr His Tyr Lys Asp Tyr Val 1460 1465 1470 Asp His Ile Phe Glu Lys Phe Glu Ser Lys Asp Glu Ser Asn Tyr 1475 1480 1485 Leu Asn Leu Asn Thr Ile Asp Val Arg Ser Tyr Lys Thr Leu Gln 1490 1495 1500 Ile Leu Leu Asp Asn Ala Val His Asn Lys Asn Asp His Trp Phe 1505 1510 1515 Asn Glu Gln Lys Asp Val His Asp Lys Asp Glu Gln Asn Lys Gly 1520 1525 1530 Thr Ile Tyr Tyr Glu Gln Asn Glu His Asp Ile Lys Ile Asp Asp 1535 1540 1545 Asn Asn Glu Glu Glu Asn Asn Asp His Thr Asp Asn Asn Asn Lys 1550 1555 1560 Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Lys Asn Asn Asn 1565 1570 1575 Asn Asn Asn Asn Asn Asn Lys Asn Asn Met Val Asp Gly Gln Ile 1580 1585 1590 Glu Cys Asn Glu Asn Val Thr Glu Lys Glu Ile Pro Ser Asn Val 1595 1600 1605 Pro Asn Ser Asn Asn Leu Thr Asn Glu Ser Ser Asn Asp Asn His 1610 1615 1620 Asn Lys Thr Thr Ile Glu His Asn Asn Asn Phe Cys Glu Glu Gln 1625 1630 1635 Asn Lys Asn Asp Leu Ile Lys Thr Ser Phe Asn Ile Thr His Cys 1640 1645 1650 Glu Glu Glu Asn Ala Asn Lys Asn Lys Tyr Thr Glu Glu Asp Lys 1655 1660 1665 Lys Glu Ile Lys Gly Asn Asp Lys Lys Asn Val Glu Met Cys Arg 1670 1675 1680 Lys Asp Thr Leu Asp Tyr Val Lys Glu Asp Ile Asn Lys Tyr Lys 1685 1690 1695 Met Tyr Ile Asn Glu Asn Asn Asn Lys Lys Glu Glu Glu Tyr Ser 1700 1705 1710 His Asn Asn Asn Asn His His His His Tyr His His Asn Asn Met 1715 1720 1725 Leu Lys Asp Asp Thr Ser Asn Tyr Asn His Val Gln Tyr Phe His 1730 1735 1740 Gly Glu Lys Lys Glu Ile Ile Cys Phe Ile Asn Lys Ser Lys Ile 1745 1750 1755 His Phe Asn Asn Tyr Lys Lys Ser Lys Arg Met Ile Ser Ile His 1760 1765 1770 Leu Ser Asp Val Leu Met Tyr Leu Phe Ser Asp Asn Asn Ile Thr 1775 1780 1785 Asn Phe Leu Phe Asn Leu Lys Lys Ile Asn Ile Leu Asn Cys Leu 1790 1795 1800 Ser His Glu Asp Ser Tyr Asn Ser Phe Phe Ser Tyr Ile Gln His 1805 1810 1815 Lys Lys Phe Ser Thr His Lys Ile Ser Lys Lys Gly Ser Lys Leu 1820 1825 1830 Ser Asp Lys Arg Lys Ser Ser Asn Val Asn Gly Ile Tyr Phe Tyr 1835 1840 1845 Asn Ser Val Phe Lys Leu Ile Gln Asn Gly Thr Phe Leu Leu Lys 1850 1855 1860 Tyr Ser Ser Lys Asn Asn Ile Ser Cys Leu Ser Ser Ile His Leu 1865 1870 1875 Asn Pro Asn His Asn His Tyr Ile Glu Asn Asn Asn Ser Lys Glu 1880 1885 1890 Lys Asn Tyr Phe Leu Asn Asn Asn Asn Asn Asn Asn Asn Lys Met 1895 1900 1905 Glu Ile Glu Glu Thr Thr Glu Phe Arg Tyr Phe Asn Thr Asn Asn 1910 1915 1920 Phe Glu Gln Leu Asn Asn Thr Ser Gly Asn Lys Ser Ile Asp Gly 1925 1930 1935 Ser Asn Val Lys Ile Asn Asn Ser Leu Asp Phe Val His Lys Asn 1940 1945 1950 Tyr Tyr Asn Asn Leu Asn Asn Asp Tyr Phe Asn Phe Asn Val Pro 1955 1960 1965 Gln Asp Ser Asn Lys Arg Lys Asp Ala Thr Ser Ser Pro Ser Asn 1970 1975 1980 Val Ser Asn Lys Ser Gly Glu Ile Lys Ser Ser His Lys Lys Glu 1985 1990 1995 Gln Arg Lys Asp Asn His Met Asn Glu Asn Ile Arg Gly Ser His 2000 2005 2010 Arg Ser Tyr Ser Thr His Gln Ser Tyr Arg Thr His Gln Ser Tyr 2015 2020 2025 Lys Arg His Ala Asn Asn Ser Asn Ile Arg Cys Ser Ser Ser Pro 2030 2035 2040 Ser Glu Arg Thr His Ile Ser Lys Lys Asp Glu Ile Lys Phe Asn 2045 2050 2055 Lys Glu Asn Ile Met Thr Leu Leu Asn Asp Cys Thr Asn Asn Glu 2060 2065 2070 Gly Glu Asn His His Val Lys Ile Thr Asn Lys Gln Ile Lys Arg 2075 2080 2085 Ser Lys Ser Lys Glu Gln Phe Glu Asp Ile Ile Asn Ser Asn Thr 2090 2095 2100 Lys Lys Thr Asn Asp Ile Glu His Ile Trp Asp Glu Gln Asn Asn 2105 2110 2115 Gln Asn Asn Lys Glu Asn Asp Asn Val Val Asn Arg Val Glu Lys 2120 2125 2130 Lys Ser Ile Ile Thr Glu Asn Tyr Ile Pro Tyr Lys Ser Cys Ile 2135 2140 2145 Glu Gln Ile Asp Asp Ile Asp Tyr Asn Lys Glu Ser Leu Gln His 2150 2155 2160 Glu Lys Asn Ile Asp Gly Lys Thr Asn Ile Ile Tyr His Asn Asn 2165 2170 2175 Asn Asn Asn Asn Asp His Ile Asn Met Asn Thr Thr Thr Cys Ile 2180 2185 2190 Pro Ile Asn Glu Lys Asn Phe Val Ile Gln Arg Asn Lys Lys Ile 2195 2200 2205 Ser His Tyr Tyr Asn Ala Lys Glu Tyr Asn Asp Ser Asn Lys Arg 2210 2215 2220 Arg Arg Asn Asn Tyr Ile Leu Thr Gly Glu Val Asn Asn Ile Lys 2225 2230 2235 Gly Tyr Asn Lys Asp Asp Met His Ser Ser His Val Asn Asn Met 2240 2245 2250 Asn Asn Ser Leu Cys Ser Ser Ala Tyr His Asn Met Lys Tyr Glu 2255 2260 2265 Lys Tyr Val Asp Ile Glu Lys Tyr Arg Asn Asp Asp Glu Phe Tyr 2270 2275 2280 Glu Ala Glu Asp Asp Glu Asp Glu Glu Asn Asp Ile Asp Asn Gln 2285 2290 2295 Glu Glu Glu Tyr Ile Asp Met Lys Lys Lys Glu Asn Glu Asn Ile 2300 2305 2310 Ser Ser Ile Glu Phe His Met Ile Asn Ser Glu Phe Ile Val Asp 2315 2320 2325 Tyr Leu Tyr Phe Ile Tyr Leu Phe Lys Trp Ile Asn Lys Leu Tyr 2330 2335 2340 Arg Lys Tyr Glu Lys Ile Lys Gln Leu Ser Asp Phe Ala Tyr Ser 2345 2350 2355 Asn Asn Asn Ser Thr Asp Asp Glu Glu Ser Asn Ile Asn Val Ser 2360 2365 2370 Lys Cys Asn Tyr Ile Leu Phe Leu Gln Asn Asn Lys Ile Tyr Val 2375 2380 2385 Pro Ile Tyr Asn Tyr Ser Ile Ser Gln Tyr Lys Tyr Tyr Asn Ser 2390 2395 2400 Leu Lys Gly Thr Ser Tyr Val Ser Leu Leu Pro Val Leu Glu Leu 2405 2410 2415 His Phe Ser Cys Ser Tyr Lys Ser Met Asn Glu Asn Asp Glu Asn 2420 2425 2430 Val Lys Ser Ile Asn Val Arg Asn Leu Gly Ser Ala Leu Val Ile 2435 2440 2445 Pro Ser Ile Asn Ile Tyr Lys His Lys Lys Asn Lys Lys Ser Asn 2450 2455 2460 Ile Cys Val Ile Thr Phe Asn Arg Lys Asp Lys Asn Asn Glu Ser 2465 2470 2475 Ile Ile Asn Asp Leu Arg Phe Gln Ile Asn Met Arg Arg Asn Tyr 2480 2485 2490 Asn Tyr Glu Ile Ile Glu Asn Glu Asp Asp Asn Ser Ser Ser Cys 2495 2500 2505 Asn Asn Met Glu Ile Pro Tyr Tyr Glu Pro Lys Tyr Thr Ser Leu

2510 2515 2520 Asn Thr Tyr Cys Thr Val Ile Ser Lys Glu Gln Met Tyr Ser Glu 2525 2530 2535 Lys Asn Lys Asn Lys Asn Asn Asn Ile Asn Asn Asn Asn Asn Asp 2540 2545 2550 Asn Asn Ile Ile Met Tyr Asp Asn Asn Met Tyr Asn Tyr His Gly 2555 2560 2565 Tyr His Thr Lys Thr Gly Ser Tyr Pro Lys Lys Ile Ile Ser Phe 2570 2575 2580 His Cys Asp Pro Ile Ile Leu Asn Phe Ser Lys Asp Ile Leu Lys 2585 2590 2595 Asn Val Leu Ile Phe Tyr Asn Thr Phe Cys Tyr Phe Ile Asn Asn 2600 2605 2610 Cys Ser Lys Asn Asp Asp Thr Gly Asn Asp Ser Asn Met Ile Arg 2615 2620 2625 Asp Met Leu Tyr Tyr Leu Tyr Asn Gln Ser Ser Asp Ile Leu Phe 2630 2635 2640 Glu Tyr Phe Leu Asp Asp Ile Arg Leu Asn Cys Tyr Asp Gly Asn 2645 2650 2655 Ile Asn Arg Asn Val Pro Leu Ile Val Val Ser Leu Phe Asn Leu 2660 2665 2670 Lys Phe Phe Leu Gln Lys Asp Ile Ile Ser Ile Tyr Asn Phe Leu 2675 2680 2685 Asn Phe Tyr Leu Asn Ile Asp Ile Tyr Asp Ser Tyr Lys Ile Leu 2690 2695 2700 Tyr Asp Thr Leu Leu Glu Lys Met Glu Ile Tyr Leu Thr Phe Glu 2705 2710 2715 Asn Asn Arg Asn Lys Leu Asn Ile Leu Asn Leu Thr Leu Glu Thr 2720 2725 2730 Ser Tyr Ile Asn Ile Leu Tyr Thr Asn Lys Ser Lys Asn Ile Leu 2735 2740 2745 Ile Asn Phe Leu Glu Pro Ile Leu Asn Lys Asn Phe Tyr Tyr Phe 2750 2755 2760 Ser Ser Ser Tyr Tyr Asn Asn Leu Asp Ile Thr Lys Asn Lys Arg 2765 2770 2775 Arg Lys Arg Ser Ile Tyr His His His Asn His Asn Tyr His His 2780 2785 2790 Glu Cys Tyr Glu Asn Asn Ile Leu Ile Lys Asn Thr Lys Gly Lys 2795 2800 2805 Ser Leu Val Thr His Ile Thr Ser Lys Ile Phe Asn Tyr Thr Gly 2810 2815 2820 Leu Ser Ile Asn Val Arg Phe Lys Gln Ser Leu Arg Asn Lys Ile 2825 2830 2835 Gln Asn Asn Ser Leu Asn Asn Lys Asn Lys Asn Lys Tyr Leu Phe 2840 2845 2850 Tyr Glu Leu Gln Asn Asn Glu His Gly Ala Leu Leu Asn Asp Glu 2855 2860 2865 Asn Gly Glu Val Cys Glu Val Val Val Ile Ile Lys Ile Phe Asn 2870 2875 2880 Tyr Ile Phe Glu Ile Ile Asp Ile Glu Arg Asn Lys Asn Thr Cys 2885 2890 2895 Asp Ile Arg Phe Leu Pro Ile Ile Tyr Glu Leu Lys Lys Phe Asn 2900 2905 2910 Glu Lys Lys Lys Ile Thr Ile Lys Lys Tyr Asp Ile Asn Tyr Asp 2915 2920 2925 Ile Tyr Asn Glu Tyr Ser Leu Lys Asn Lys Val Phe Lys Thr Phe 2930 2935 2940 Ile Lys Leu Tyr Ile Gln Thr Asn Ile Asp Glu Lys Gly Ile Val 2945 2950 2955 Ser Ile Tyr Phe Ser Ser Asn Leu Phe Ile Asn Asn Ser Thr Asn 2960 2965 2970 Ser Asp Phe Val Leu Leu Phe His Pro Val Leu Tyr Tyr Phe Met 2975 2980 2985 Leu Phe Asn Glu Gln Ser Thr Asn Ser Asn Thr Asn Asn Asn Ile 2990 2995 3000 Asn Asn Asn Asn Lys Asn Glu Asp Asn Met Val Asp Asn Asn Glu 3005 3010 3015 Asn Asn Asp Lys Asn Asn Asp Lys Asn Asn Asp Lys Asn Asn Asn 3020 3025 3030 Glu Asn Lys Asp Cys Asp Ser Val His Asn Ile Asn Asn Val Glu 3035 3040 3045 Ser Asp Ser Pro Val Asn Ile Asn Phe Phe Lys Asn Asp Asn Tyr 3050 3055 3060 Asn Asn Asn Tyr His Asn Asn Tyr His Asn Asn Tyr His Asn Asn 3065 3070 3075 Asn Asp Lys Gly Ala Asn Ile Thr Ile Ile Asn Ser Asn Glu Arg 3080 3085 3090 Tyr Phe Leu Pro Leu Ile Ser Phe Ile Pro Ile Ile Ile Leu Tyr 3095 3100 3105 Asp Tyr Leu Lys Lys Lys Asn Glu Ile Glu Glu Asn Asn Tyr Pro 3110 3115 3120 Asp Lys Tyr Pro Met Asn Ser Lys Asn Ser Tyr Val Thr Pro Val 3125 3130 3135 Ser Met Val Cys Asp Leu Lys Gly Ser Phe Ser Ala Asp Ile Lys 3140 3145 3150 Lys Asn Asn Phe Ile Lys Arg Phe Asn Ser Leu Asn Tyr Asn Met 3155 3160 3165 Asn Ile His Asn Gly Glu Glu Glu Glu Ile Ser Lys Gln Asn Glu 3170 3175 3180 Ala Asn Asp Met Leu Asn Tyr Thr His Val Asp Asn Ser Asn Asn 3185 3190 3195 Glu Thr Ser Cys Arg Asn Asn Asn Glu Asp Lys Ile Leu Ser Tyr 3200 3205 3210 Lys Lys Arg Ile Thr Tyr Asp Tyr Ser Ser Ile Asn Ser Leu Lys 3215 3220 3225 Lys Leu Ser Tyr Asn Asn Leu Thr Arg Ala Ser Cys Tyr Val Asn 3230 3235 3240 Ser Arg Ser Ser Glu Met Asn Leu Cys Asn Ile Ile Ser Asn Lys 3245 3250 3255 Gly Asp Lys Arg Asn Phe Met Asn Asn Glu Lys Ile Ile Glu Ser 3260 3265 3270 Leu Asn Leu Asn Ala Leu Arg Glu Ile Asn Lys Ile Arg Thr Asn 3275 3280 3285 Arg Ile Leu Lys Arg Leu Glu Lys Tyr Val Asn Glu Lys Ser Asn 3290 3295 3300 Ser Lys Asn Met Lys Asn Ile Asn Tyr Ile Tyr Ile Ile Lys Lys 3305 3310 3315 Glu Phe Tyr Asp Phe Tyr Ile His Lys Asn Glu Asn Asn Asn Lys 3320 3325 3330 Cys Tyr Phe Glu Met Phe Asn Phe Leu Ile Lys Asn Gly Ala Lys 3335 3340 3345 Lys Val Leu Asn Asn Ser Ser Ile Lys Glu Ile Ile Cys Arg His 3350 3355 3360 Ser Phe Asp Phe Leu Lys Tyr Asn Leu Tyr Ala Gln Thr Leu Lys 3365 3370 3375 Leu Lys Thr Leu Lys Ile Ile Cys Tyr Asp Lys Tyr Ser Arg Tyr 3380 3385 3390 Leu Arg Glu Lys Lys Lys Glu Met Glu Glu Ala Lys Thr Tyr Asn 3395 3400 3405 Asn Asn Asn Asn Lys Asn Asn Ile Asn Asn Asn Asn Asn Asn Asn 3410 3415 3420 Asp Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn 3425 3430 3435 Asn Asn Asn Asn Met Lys Lys Lys Gln Ile Gly Asp Ile Tyr Met 3440 3445 3450 Lys Asn Tyr Lys Glu Glu Lys Asn Asp Ile Lys Glu Glu Gln Glu 3455 3460 3465 Lys His Asp Asn Ser Ser Ser Ile Val Val Leu Asn Lys Phe His 3470 3475 3480 Asp Tyr Tyr Asp Lys Arg Asp Lys Tyr Tyr Phe Glu Asn Asn His 3485 3490 3495 Arg Asn Asp His Tyr Thr Ser Asp Leu Asn Asn Asp Asp Asn Asn 3500 3505 3510 Lys Tyr Asn Ile Tyr Asp Asn Asn Lys Tyr Ser Ile Tyr Asp Asn 3515 3520 3525 Asn Lys Tyr His Ile Tyr Asp Asn Asn Glu Ser Asn Asn Lys Lys 3530 3535 3540 Asp Asp Phe Tyr Tyr Ser Ile Pro Thr Asn Leu Asp Ile Ser Leu 3545 3550 3555 Lys Cys Ile Asn Arg Asn Glu Arg His Ile Asn Ser Leu Leu Phe 3560 3565 3570 Asn His Ser Lys Arg Lys Ser Thr Leu Ser Asn Ile Leu Ile Lys 3575 3580 3585 His Asp Met Ile Lys Asn Ser Val Ser Lys His Ile Leu Asn Asn 3590 3595 3600 Asp Tyr Ala Tyr Glu Tyr Asp Lys Lys Glu Glu Ile Glu Tyr Asn 3605 3610 3615 Tyr Asn Asn Lys Asp Asn Cys Glu Lys Asn Ile Tyr Val Leu Asp 3620 3625 3630 Val Lys Asn Lys Lys Gln Asn Asn Asn Asn Asn Met Asn Asp Asp 3635 3640 3645 Asp Asp Asp Asn Asn Asn Met Asn Asp Asp Asp Asp Asp Asn Asn 3650 3655 3660 Asn Met Asn Asp Asp Asp Asp Asp Asn Asn Asn Met Asn Asp Asp 3665 3670 3675 Asp Asn Asn Asn Asp Asn Asn Asn Asp Lys Lys Lys His His Ile 3680 3685 3690 Ile His Tyr Asn Asn Asn Asn Ile Ile Lys Lys Lys Arg Arg Lys 3695 3700 3705 Phe Ile Phe Thr Ala Lys Lys Gln Ser Ser Tyr Ser Asn Thr Ile 3710 3715 3720 Ile Cys Glu Gln Lys Asn Ile Asp Val Asn Lys Ile Tyr Ser Phe 3725 3730 3735 Gly Ile Tyr Ile Asp Tyr Ile Phe Glu Ile His Asn Phe Met His 3740 3745 3750 Glu Glu Met Lys Ile Gly Tyr Cys Gln Lys Tyr Tyr Ile Asn Ser 3755 3760 3765 Ile Pro Lys Lys Ile Lys Arg Ile Lys Arg Glu Glu Ile Ser Pro 3770 3775 3780 Lys Ser Ile Lys Tyr Leu Asp Thr Ile Pro Lys Tyr Ile Lys Leu 3785 3790 3795 Phe Tyr Val Leu Asn Asn Asp Asn Tyr Ile Phe Asn Phe Phe Ser 3800 3805 3810 Lys Lys Ile Gln Leu Tyr Lys Tyr Ala Asn Thr Pro Asp Lys Ile 3815 3820 3825 His Gln Ile Lys Met Tyr Ile Glu Tyr Ile Asn Asp Glu Asn Lys 3830 3835 3840 Asn Ile Ile Tyr Lys Lys Arg Lys Tyr Lys Leu Asn Ser Leu Phe 3845 3850 3855 Lys Arg Lys Ser Lys Asn Lys Arg Lys Tyr Lys Tyr Leu Glu Ser 3860 3865 3870 Lys Asp Tyr Asp Lys Leu Cys Lys Glu Val Pro Lys Phe Phe Tyr 3875 3880 3885 Leu Asn Ile Thr Phe Arg Lys Lys Thr Asp His Ser Asn Asn Thr 3890 3895 3900 Tyr Tyr Asn Glu Asn Asn Phe Lys His Val Ile Asp Ser Asn Val 3905 3910 3915 Leu Gln Leu Leu Ile Tyr Pro Ser Phe Phe Phe Gln Asn Asn Leu 3920 3925 3930 Asp Asn Asp Leu Ile Ile Lys Ser Asn Gly Asn Leu Gln Lys Ile 3935 3940 3945 Lys Lys Lys Ser Ile Met Phe Phe Gly Asp Val Asp Asn Ser Lys 3950 3955 3960 Val Ile Leu Gln Ile Val Tyr Asn Asn Gln Ile Phe Leu Ser Lys 3965 3970 3975 Lys Ile Lys Ile Asn Gln Val Cys Leu Asn Lys Lys Ile Ile Leu 3980 3985 3990 Thr Ser Lys Asn Lys Lys Glu Asn Ile Phe Leu Ser Met Asn Ile 3995 4000 4005 Thr Leu Asn Gly Asn Ile Tyr Asn Thr Lys Thr Ile Thr Ile Asn 4010 4015 4020 Asn Arg Tyr Thr Ile Ile Asn Asn Thr Asn Arg Thr Leu Tyr Val 4025 4030 4035 Gln Glu Val Asn Lys Asp Phe Lys Ile Ile Lys Lys Lys Glu Lys 4040 4045 4050 Lys Tyr Met Thr Tyr Ser Phe Ser Leu Glu Asn Leu Gln Asn Gln 4055 4060 4065 Asn Asn His Ser Asn Asp Glu Asn Met Glu Lys Leu Tyr Ile Pro 4070 4075 4080 Ser Ser Asn Asp Leu Thr Asn Ile Lys Arg Asn Tyr Asn Gln Lys 4085 4090 4095 Glu Lys Asn Glu Lys Asn Glu Lys Lys Glu Lys Asn Glu Asn Leu 4100 4105 4110 Asn Lys Ile Thr Ile Asn Lys Arg Ser Asp Leu Thr Tyr Glu His 4115 4120 4125 Glu Asn Asp Glu Thr Lys Ile Met Asp Thr Cys Glu Ile Ser Asp 4130 4135 4140 Lys Lys Tyr Thr Glu Lys Gly Met Thr Lys Ile Cys Glu Phe Asn 4145 4150 4155 Lys Lys His Ile Lys Tyr Asn Lys His Lys Lys Tyr Asn Asp Lys 4160 4165 4170 Lys Tyr Asn Gln Lys Glu Asn Asn Asn Asn Asn Asn Asn Thr Thr 4175 4180 4185 Ser Ser Asn Asn Ser Asn Ser Asn Ser Asn Ser Ser Asn Asn Arg 4190 4195 4200 Pro Asn Ile Ile Ser Ile Lys Pro Tyr Glu Ser Phe Tyr Tyr His 4205 4210 4215 Pro Ile Asn Thr Asn Lys Cys Phe Leu Thr Phe Ser Tyr Thr Asn 4220 4225 4230 Ile Met Glu Cys Lys Asn Asp Lys Asn Phe Tyr Arg Arg Arg Gly 4235 4240 4245 Ser Tyr Glu Phe Thr His Thr Gln Asn Glu Ile Thr Asn Leu Phe 4250 4255 4260 Asn Asn Tyr Leu Lys Lys Lys Ile Met Lys Ser Gln Asn Ile Ser 4265 4270 4275 Ile Ile Asn Asp Lys Glu Glu Asn Ile Asn Asn Asn Val His Lys 4280 4285 4290 Asn Gln Glu Phe Tyr Asn Leu Phe Tyr Thr Ser Pro Ile Glu Ile 4295 4300 4305 Glu Lys Val Gly Asn Tyr Lys Ile Phe His Lys Thr Val His Lys 4310 4315 4320 Ile Asp Val Thr Tyr Asn Asn Glu Lys Asp Ala Thr Asp Asp Gln 4325 4330 4335 Pro Thr Tyr Glu Asp Asp Ile Ile Gln Lys Glu Ile Lys Gln Gln 4340 4345 4350 Glu Lys Glu Ile Glu Glu Glu Ile Lys Arg Glu Ile Glu Glu Leu 4355 4360 4365 Glu Glu His Arg Glu Lys Glu Thr Ile Ile Val Phe Ser Asn Leu 4370 4375 4380 Lys Asp Ser Tyr Glu Lys Glu Met Gln Lys Lys Lys Lys Asp Arg 4385 4390 4395 His Lys Lys Asn Asn Phe Leu Asn Ile Asn Ser Asp His Ile Asn 4400 4405 4410 Asp Lys Asn Ile Ile Asn Ser Tyr Lys Asn Glu Gln Thr Thr Glu 4415 4420 4425 Met Ile Tyr Ile Gln His Asp Tyr Thr Asp Asn Ile Pro Ala Asp 4430 4435 4440 Asp Glu Lys Lys Ser Ser His Asn Glu Thr Ile Leu Ile Asp Asp 4445 4450 4455 Leu Thr Leu Phe Asn Lys Asn Asn Lys His Ser Ile Asn Glu Arg 4460 4465 4470 Glu Asn Lys Glu Asn Ile Phe Asn Glu Thr Val Leu Thr Val Asp 4475 4480 4485 Asn Asn Asp Thr Glu His Ile Lys Ser Asp Tyr Val Asn Ile Lys 4490 4495 4500 Gly Asp Cys Ile Asn Ile Lys Gly Asp Cys Ile Asn Ile Lys Gly 4505 4510 4515 Asp Tyr Val Asn Ile Lys Asp Asn Trp Val Asp Ile Lys Asp Asn 4520 4525 4530 Cys Val Asn Ile Lys Asp Asn Cys Val Asn Ile Lys Asp Asn Cys 4535 4540 4545 Val Asn Ile Lys Gly Asn Cys Val Asn Thr Lys Glu Asp Ser His 4550 4555 4560 Asn Asn Tyr Gln Gln Asn Gly Met Asn Leu Arg His Asp Ser Phe 4565 4570 4575 Cys Glu Asn Tyr Val Ile Asp Phe Asn Asp Asp Asp Ser Cys Ile 4580 4585 4590 His Phe Lys Lys Asn Lys Asn Asp Ile Asn Thr Ala Asp Asp Ile 4595 4600 4605 Asn Asn Asn Thr Val Ile Ile Pro Ile Lys Ser Ile Lys Gln Leu 4610 4615 4620 Glu Gly Glu Glu Asn Val His Asn Ile Glu Asn Lys Asn Asn Asn 4625 4630 4635 Ser Asn Asn Asn Asn Asn Asn Leu Leu Cys Glu Leu Asn Lys Thr 4640 4645 4650 Ser Asp Asn Lys Glu Arg Ile Asn Thr Lys His Met Phe Leu Tyr 4655 4660 4665 Lys Glu Glu Ser Ile Ile Thr Glu Ile Asn Ile Ala Leu Asn Ser 4670 4675 4680 Asn Thr Asn Ile Glu Ile Asn Ile Ser Ile Glu Asn Asn Pro Asp 4685 4690 4695 Thr Ile Ile Tyr Asn Lys Thr Asn Tyr Tyr Leu Ile Phe Tyr Gln 4700 4705 4710

Arg Lys Thr Asn Lys Lys Ile Val Asn Leu Leu Lys Pro Phe Asp 4715 4720 4725 Lys Glu Ile Phe Gly Trp Thr Asp Val Cys Lys Pro Lys Val Ile 4730 4735 4740 Lys Cys Phe Leu Ile Ile Gln Lys Asn Asp Val Tyr Val Phe Ser 4745 4750 4755 Cys Asn Leu Asn Ile Val Lys Glu His Asn Asn Ile Ile Leu Asn 4760 4765 4770 Asn Asn Arg Arg Ile Ile Val Leu Thr Lys Ile Glu Asn Gly Lys 4775 4780 4785 Arg Val Phe Cys Leu Glu Glu Met Asn Ile Glu Leu Leu Asp Cys 4790 4795 4800 Asn Glu Lys Tyr Glu His Lys Lys Ser Leu Ala Pro Phe Phe Ser 4805 4810 4815 Ala Ser Lys Gly Phe Asn Arg Phe Arg Glu Lys Lys Lys Lys Lys 4820 4825 4830 Ile Lys Lys Lys Leu His Lys Ile Ser Leu Tyr Asn Ser Leu Ile 4835 4840 4845 Lys Ser Ser Gln Met Arg Lys Glu Lys Asn Lys Ser Lys Asn Lys 4850 4855 4860 Lys Lys Asn Lys Lys Gln Lys Lys Asn Gln Met Asn Gln Met Asn 4865 4870 4875 Gln Thr Asn Gln Thr Asn His Met Leu Gln Lys Asn Lys Gln Thr 4880 4885 4890 Asn Asp Asn Tyr Leu Val Lys Tyr Lys Asn Glu Lys Phe Asn Lys 4895 4900 4905 Lys Leu Pro Leu Leu Ser Lys Lys Arg Phe Asn Ile Ala Tyr Leu 4910 4915 4920 Lys Lys Lys Arg Lys Gln Ile Lys Lys Lys Lys Asn Asn Lys Tyr 4925 4930 4935 Phe His Leu Ser Ser Phe Phe Leu Leu Lys Ser Met Asp Ser Ser 4940 4945 4950 Ile His Lys Asp Val Glu Lys Glu Arg Lys Lys Gln Lys Gly Lys 4955 4960 4965 Ser Gln Gln Ala Glu Lys Leu Phe Lys Val Glu Lys Ile Val His 4970 4975 4980 Asn Glu Ser Tyr Asn Glu Ser Tyr Asn Glu Ser Tyr Asn Glu Tyr 4985 4990 4995 His Asn Glu Tyr His Asn Glu Tyr His Asn Glu Tyr His Asn Glu 5000 5005 5010 Tyr His Asn Glu Asn Asn Gly Asn Ile Asn Ser Ile Lys Tyr Asn 5015 5020 5025 Asn Ile Ile Lys Glu Glu Glu Asn Val Lys Asn Lys Asn Ala Thr 5030 5035 5040 Ser Asn Asn Asn Ser Ser Phe Ser Lys Lys Asn Ile Lys Leu Asn 5045 5050 5055 Asn Val Ser Ile Lys Lys Asp Asp Lys Lys Lys Ser Thr Ser Lys 5060 5065 5070 Tyr Lys Ser Leu Asn Ser Lys Lys His Ile Asn Lys Phe Phe Asn 5075 5080 5085 Asp Asn Tyr Thr Phe Arg Lys Lys Lys Lys Arg Lys Lys Ser Asn 5090 5095 5100 Lys Lys Asn Leu Tyr Val Asn Lys Asn Asn Glu Glu Thr Glu Thr 5105 5110 5115 Ser Asp Tyr Tyr Ile Glu His Asn Glu Asn Lys Lys Asn Ile Glu 5120 5125 5130 Leu Ser Ser His Asp Asn Asn Ser Phe Asp Asn Glu Lys Arg Lys 5135 5140 5145 Thr Ser Leu Gly Met Thr Lys Ser Lys Gly Tyr Asn Asp Leu Phe 5150 5155 5160 Thr Val Lys Phe Arg Lys Ser Val Asn Glu Asn Asp Lys Ile Asp 5165 5170 5175 Glu Tyr Lys Glu Asn Ser Phe Glu Asp Ser Ser Thr Tyr Asn Asp 5180 5185 5190 Ser Phe Leu Ser Ser Ser Ser Asn Asn Glu Asn Tyr Ile Ser Asn 5195 5200 5205 Asn Asn Lys Lys His Met Phe Leu Asn Arg Tyr Tyr Asp Asn Lys 5210 5215 5220 Asp Asp Lys Lys Lys Tyr Arg Glu Tyr Asn Leu Ile Tyr Asn Lys 5225 5230 5235 Glu Met Lys Ala Lys Asn Asn Leu Lys Lys Pro Lys Lys Lys Ile 5240 5245 5250 Lys Arg Lys Leu Ser Asp Phe Ile Phe Thr Lys Ile Lys Lys Asn 5255 5260 5265 Gln Lys Lys Phe Lys Gly Leu Ser Arg Ile Tyr Pro Met Phe Leu 5270 5275 5280 Lys Ser Asn Lys Ser Asp Glu Phe Asn Val Leu Ile Arg Asn Tyr 5285 5290 5295 Leu Ser Asp Lys Asp Asp Ser Thr Ser Ile Arg Lys Glu Tyr Lys 5300 5305 5310 Ile Ile Asn Asp Asn Tyr Ile Asn Asn Asn Glu Asn Asn Asn Asn 5315 5320 5325 Glu Asn Met Asn Tyr Asp Leu Lys Thr Asn Thr Tyr Asn Asn Ile 5330 5335 5340 Leu His Asn Asn Asn Tyr Arg Leu Asp Lys Lys Ser Val Phe Glu 5345 5350 5355 Glu Asn Arg Lys Ile Pro Ile Lys Phe Ile Thr Asn Leu Asn Val 5360 5365 5370 Met Leu Arg Gly Ile Lys Ile Ser Leu Phe Glu His Thr Cys Asp 5375 5380 5385 Tyr Val Ile Ser Phe Glu Ile Asn Glu Leu Leu Val Thr Lys Glu 5390 5395 5400 Tyr Met Glu Tyr Ile Asp Tyr Tyr Asn Val Asp Ile Lys Ile Asn 5405 5410 5415 Asn Ile Leu Cys Tyr Ala Val Tyr Lys Tyr Leu Ser Ala Cys Ala 5420 5425 5430 Val Leu Tyr Thr Asn Asn Asp Ser Asn Ile Lys Asn Asn His Leu 5435 5440 5445 Arg Met Leu Met Asn Glu Leu Lys Ile Lys Arg Asn Leu Phe Leu 5450 5455 5460 Gln Lys Lys Arg Arg Asn Glu Asn Lys Lys Glu Tyr Ser Asp Val 5465 5470 5475 Asn Ile Asp Lys Asn Asn Asp Asn Ile Lys Asn Asn Asp Asn Ile 5480 5485 5490 Lys Asn Asn Asp His Ile Asn Asp Asn Asp His Ile Asn Asp Asn 5495 5500 5505 Asp His Ile Asn Asp Lys Asp His Ile Asn Asn Lys Asp His Ile 5510 5515 5520 Asn Asn Lys Asp His Ile Asn Asp Asn Glu Lys Glu Met Tyr Ser 5525 5530 5535 Ser Gly Ser Ser Arg Thr Ser Tyr Thr Asn Lys Lys Lys Gly Tyr 5540 5545 5550 Lys Tyr Lys Asn Asn Tyr Lys Lys Lys Lys Lys Lys Thr Glu Lys 5555 5560 5565 Asn Ile Tyr Asp Asn Lys Lys Ala Lys Lys Lys Lys Lys Glu Lys 5570 5575 5580 Val Glu Lys Lys Lys Lys Asn Leu Phe Val Asn Asn Glu Phe Leu 5585 5590 5595 Ile Cys Asn Phe Gln Tyr Val Arg Lys Cys Glu Thr Leu Phe Phe 5600 5605 5610 Lys Tyr Phe Leu Leu Ser Leu Lys Pro Ile Val Ile Asn Ala Asp 5615 5620 5625 Ile Asn Ser Ile Thr Ile Leu Phe Tyr Phe Tyr Lys Thr Phe Tyr 5630 5635 5640 Asn Phe His Thr Lys Lys Asn Glu Gln Gly Gln Gly Val Gln Asn 5645 5650 5655 Gly Phe Asn Thr Asp Asn Tyr Glu Lys Val His Met Arg Glu Ile 5660 5665 5670 Asn Met Met Lys Glu Gln Gln Gln Gln Gln Lys Lys Lys Lys Lys 5675 5680 5685 Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu 5690 5695 5700 Lys Lys Lys Lys Asn Lys Glu Asn Val Leu Gln Lys Lys Lys Thr 5705 5710 5715 Lys Gln Glu Met Glu Arg Pro Leu Lys Asp Tyr Tyr Tyr Tyr Tyr 5720 5725 5730 Tyr Ser Ser Met Asn Ile Leu Asn Asp Pro Asp Asn Asn Lys Arg 5735 5740 5745 Cys Asp Ile Gln Lys Asn Arg Met Ile Glu Lys Asn Asp Ser Phe 5750 5755 5760 Phe Ile Asn Val Asp Glu Asn Arg Lys Gln Lys Ile Gln Asp Ile 5765 5770 5775 Thr Gly Asp His Ile Ile Asp Asn Asn Ile Leu Ser Arg Met Lys 5780 5785 5790 Asn Met Glu Ser Phe Ile Ile His Glu Glu Gln Gln Lys Asn Asp 5795 5800 5805 Leu Thr Leu Thr Leu His Asn Asn Ile Tyr Asn Val Glu Gly Glu 5810 5815 5820 Tyr Tyr Asn Ile Lys Lys Ser Asn Met Asn Tyr Tyr Asn Asn Ile 5825 5830 5835 Ile Asn Asn Lys Lys Ser Ser Val Gln Ile Lys Lys Lys Thr Thr 5840 5845 5850 Leu Ile Glu Lys Asn Asp Ile Asn Asn Lys Tyr Val Tyr Leu Gln 5855 5860 5865 Tyr Ile Ser Ile Asp Lys Ile Asn Ile Leu Leu Asn Phe Gln Ser 5870 5875 5880 Glu Tyr Lys Lys Glu Leu Thr Asn His Val Asn Asp Leu Phe Tyr 5885 5890 5895 Lys Glu His Ile Ser Ala Tyr Asn Lys Leu Gly Asp Ile Ser Asn 5900 5905 5910 Cys Asn Ile Tyr Leu Lys Ser Leu Ser Asn Ile His Ile Phe Thr 5915 5920 5925 Asn Tyr Asn Leu Leu Ile Asn Phe Leu Gln Asn Phe Tyr Tyr Asn 5930 5935 5940 Gln Thr Ile Val Asn Leu Ser Asn Leu Leu Ile Ser Phe Asn Ile 5945 5950 5955 Ile Gly Ser Pro Thr Ser Leu Ile Ala His Val Lys Asn Ala Phe 5960 5965 5970 Asn Glu Phe Phe Tyr Ile Ile Asn Glu Asp Thr Leu Ser Lys Lys 5975 5980 5985 Asn Arg Tyr Asp Asp Asp Lys Asp Ile Asn Asp Tyr Thr Thr Lys 5990 5995 6000 Tyr Asn Arg Lys Asn Asn Lys Lys Asn Asn Arg Lys Asn Asn Lys 6005 6010 6015 Lys Asn Asn Arg Lys Asn Asn Arg Lys Asn Asn Lys Asn Asn Asp 6020 6025 6030 Asn Asn Asn Ile Asn Ser Met Tyr Gly Ser His Tyr Asn Asn Asn 6035 6040 6045 Tyr Ile Tyr Tyr Asn Asp Tyr Tyr Tyr Asn Asn Asn Tyr Met Tyr 6050 6055 6060 Arg Arg Ser Thr Leu Asn Tyr His Tyr Tyr Glu Gln Gly Tyr Asp 6065 6070 6075 Lys Tyr Lys Leu Asn Arg Ile Tyr Asn Asn Asn Pro Lys Leu Arg 6080 6085 6090 Lys Asn Asn Lys Glu Arg Lys Asn Asn Ile Gln Gln Ile Asn Tyr 6095 6100 6105 Thr Thr Lys Lys Glu Asp Lys His Asn Phe Leu Asn Leu Ile Asp 6110 6115 6120 Gln Ser Arg Glu Ser Ser Phe Leu Asp Glu Lys Asp Asp Ser Leu 6125 6130 6135 Thr Asn Asp Asp Lys Tyr Ile Met His Leu Leu Asn Asp Tyr Asn 6140 6145 6150 Ser Ser Tyr Gln Asn Asn Leu Leu Ser Glu Glu Glu Ser Phe Val 6155 6160 6165 Lys Arg Asn Phe Ser Trp Asn Phe Phe Lys Lys Gln His Thr Asn 6170 6175 6180 Asn Asn Asn Asn Asn Ser Asp Asp Asp Asn Tyr Phe Asn Asp Ser 6185 6190 6195 Tyr Asn Asn Ile Lys His Glu Ser Ser Gly Asn Leu Asn Tyr Met 6200 6205 6210 Tyr Thr Lys Ser Ile Lys Asn Lys Asp Leu His Lys Leu Val Ile 6215 6220 6225 Asp Asp Asn Asn Ser Ser Met Asn Ser Phe Leu Phe Asn Asn Lys 6230 6235 6240 Lys Asn Val Ile Ile Leu Asp Gln Val Ser Lys Lys Lys Lys Lys 6245 6250 6255 Lys Leu Gln Tyr Ile Lys Asn Phe Glu Glu Pro Gln Lys Gly His 6260 6265 6270 Ile Tyr Met Asp Asp Asp Met Tyr Ile Asp Val Asp Val Asp Leu 6275 6280 6285 Asn Ser Tyr Ala Glu Gly Lys Asn Thr Thr Lys Asp Thr Ser Lys 6290 6295 6300 Tyr Pro Pro His Phe Lys Ile Ser Tyr Asp Glu Glu Lys Lys Lys 6305 6310 6315 Arg Lys Lys Lys Arg Leu Ser Thr Gly Ser Asn Asn Asn Arg Tyr 6320 6325 6330 Lys Asn Asp Ala Glu Ile Leu Asp Met Asn Pro Asn Lys Lys Asn 6335 6340 6345 Lys Lys Lys Asn Lys Met Leu Lys Asn Asn Asn Asn Asn Asn Asn 6350 6355 6360 Asn Asn Lys Asn Lys Lys Asn Lys Arg Asn Asn Lys Arg Ser Gly 6365 6370 6375 Cys Thr Lys Met Phe Arg Phe Phe Lys Lys Ile Gly Leu Asn Ile 6380 6385 6390 Ile Tyr Ile Phe Ser Lys Thr Lys Arg Ile Cys Ile Gly Ile Tyr 6395 6400 6405 Val Leu Phe Ile Gln Leu Ile Leu Ser Leu Leu Phe Ser Ile Thr 6410 6415 6420 Leu Ile Leu Gln Ser Leu Ile Asn Leu Val Glu Lys Val Asn Ile 6425 6430 6435 Asn Asn Ala Asp Asn Val Phe Leu Ile Phe Asn Lys Lys Tyr Glu 6440 6445 6450 Arg Lys Lys Val Val Val Phe Asn Asn Ile Glu Glu Tyr Asn Lys 6455 6460 6465 Phe Asn Ile Thr Lys Leu Phe Tyr Tyr Tyr Ile Ser Ile Tyr Met 6470 6475 6480 Asn Ile Val Asn Phe Leu Tyr Tyr Asn Met Leu Tyr Lys Met Lys 6485 6490 6495 Lys Lys Lys Lys Asp Leu Ser Gly Glu Asp Lys Ile Phe Met Lys 6500 6505 6510 Lys Leu Lys Ile Asn Leu Lys Asn Lys Arg Lys Phe Ser Phe Phe 6515 6520 6525 Ile Asn Phe Phe Leu Asn Ile Phe Asn Ile Ile Asn Ile Leu Leu 6530 6535 6540 Phe Gly His Ile Val Phe Leu Leu Leu Cys Leu Leu Thr Met Asn 6545 6550 6555 Asn Ile Phe Gln Lys Ile Leu Thr Ala Cys Ile Lys Leu Val Tyr 6560 6565 6570 Tyr Gln Arg Asn Lys Asn Tyr Leu Asp Phe Leu Asn Lys Asn Ser 6575 6580 6585 Phe Asn His Met Asn Ile Ile Ser Tyr Ile Lys Ile Asn Gln Val 6590 6595 6600 Ile Ser Lys Tyr Val Leu Leu Lys Glu Pro Phe Lys Tyr Phe Leu 6605 6610 6615 Ser His Gln Asp Phe Leu Asp Ile Lys Ala Pro Lys Lys Arg Asn 6620 6625 6630 Tyr Phe Val Tyr Thr Lys Glu Leu Phe Phe Tyr Ile Gln Glu Asn 6635 6640 6645 Lys Ile Val Phe Leu Phe Arg Lys Asn Asp Met Lys Lys Ile Asp 6650 6655 6660 Ile Ile Ile His Thr Ile Asn Asn Ser Phe Ala Ile Asn Ser Tyr 6665 6670 6675 Tyr Lys Lys Lys Gly Ser Lys Thr Ala Lys Lys Ser Arg Ser Lys 6680 6685 6690 Asn Thr Pro Ser Asn Tyr Thr Leu Asn Met Glu His Val Thr Gly 6695 6700 6705 Glu Glu Asn Asn Ser Phe Thr Ser Ser Leu Tyr Asn Lys Thr Asn 6710 6715 6720 Val Ser Asn Asp Ile Ser Glu Asp Lys Asn Val Ile Cys Phe Leu 6725 6730 6735 Asn Gln Glu Leu Ala Leu Thr Lys Arg Asn Glu Ile Ile His Ile 6740 6745 6750 Asn Asn Lys Asn Tyr Asp Tyr Ile Ile Ser Ile Lys Ile Thr Ile 6755 6760 6765 Lys Asn Asn Met Asn Val Met Glu Asn Ile Tyr Tyr Asn Pro Lys 6770 6775 6780 Lys Phe Phe Lys Cys Leu Ser Lys Ile Gln Lys Ser Phe Asn Asn 6785 6790 6795 Ile Tyr Leu Pro Leu Cys Lys Asn Asn Asn Asn Glu Thr Lys Asn 6800 6805 6810 Asn Lys Glu Lys His Leu Asn Asn Lys Gly Ala Asp Ile Tyr Lys 6815 6820 6825 Ala Asp Ile Asn Val Lys Lys Arg Arg Thr Phe Phe Ser Ala Ile 6830 6835 6840 Ile Cys Phe Phe Lys Ser Ile Phe Ile Phe Leu Tyr Phe Cys Leu 6845 6850 6855 Cys Pro Trp Lys Leu Leu Lys Leu Lys Lys Lys Lys Ser Asn Asp 6860 6865 6870 Asn Lys Lys Lys Gln Met Asp Glu Lys Asp Lys Glu Asn Ala Val 6875 6880 6885 His Lys Asn Ile Gln Leu Lys His Lys Asn Lys Lys Glu Lys Thr 6890 6895 6900 Thr Pro Asn Ser Ile Ile Phe Lys Leu Asn

Phe Glu Asp Phe Gln 6905 6910 6915 Ser Thr Leu Asn Ile Phe Glu Cys Leu Cys Arg Ile Met Asn Asn 6920 6925 6930 Asn 137458DNAPlasmodium falciparum 13atgttttcag tcgaattaga aaatcgatca ggttataaaa aaaggaaaaa aaagaaatgg 60aataataaaa gtactggtca ggataaattt acgaacaaag atattatatc agaggaaaaa 120gaagaaggac ttgatataga atgtggtcat aatattctgg gggatgtaca atatgatggt 180acatataata taaatgaaca agttaagaaa aattcattgt tctattttaa atgtaaagag 240gaaattaatt taaaagatgg aaatataata ttggatgata aaaatagaaa agtggatgat 300ataaatataa caggggatga taaaaatata aaagtggatg ataaaaatat aaaagtggat 360gataaaaata taacagggga ggataaaaat ataacagggg aggataaaaa tataacaggg 420gatgataaaa atataatttt tgatgttgat gaaatattaa tccatcaaca taatacatca 480aatagtaata tatatataaa ttgtaatgat aataataatg atattaggaa cagttcaaat 540gtacagcatt attataatga taaaataaaa gaaaatataa ataaacaaaa taaaaagtat 600gttttaataa atgattatat aaataataaa tatattttat caaaaaataa gacatgtaaa 660ataaataaag gaaaaaaatt aattaaaaaa aaaaaagtaa ataatatttc taggaggagg 720aatcatatat tatataaatg tcgtaataag ttatataatg gaaatgtttt ttctgacgat 780attattaaaa gtgaagtgaa tgtatgtaat tcgttaactg tacttcataa gaattataat 840attaatatgg ataattattt agatgataat atacatacaa ataatagcaa tatttatgat 900attaattata caaatgaaaa tgttattaat tcaacatgtc gttattatcc cataggtaat 960aataacacat taagtaaaga tgaagttaca aaatcaagtt caaaaattaa ttccctttct 1020tattttgatg atattattaa tgtaaataaa aatgatatac ctattttaca tgataaagaa 1080aatataaata taataagtaa caaggaaagc tgtcataagg atgaaaagga ggaagagaaa 1140tatatcatgt ataattcaaa tttagtagaa gaaaaaaaac aaaaaaaaat gatttggaat 1200tctcttaatg tattaccaat agatatacta cttaaaaatg gacatgatga aattaataaa 1260gagatatgca aaaaaaaaaa aaaaagtttt tttagtcaga atgatataaa gtctaaaatg 1320ttatacaata ataaaagtta ttcaaaaagt gaaaaagtat tatatacaaa taataaaaac 1380agtaatacgt tcattcctat atttttctta aataaggtag gagacaagtt taaaaactct 1440gagaatatat atgatatgta taataataaa aaaaatgtct atatacatga taagaagata 1500tatactaata tgtattctaa taaattaaaa cagaaacatt attatagcac ttctaatata 1560aatttattat ataataatat aggaaaggta ttagataatg gtctacattt atcgaataat 1620atgtattgtc gtttaaattc aaatccacca tataagagta tctcgttaat taataacaat 1680gttttttttt ataaaaagag gaaaagtaat agtaataata ataataataa taataatatt 1740agtagtagta gtagtagtag tagtaaaaaa aatcacgtga tcattaataa aaaaatatca 1800tcttataata ttcattataa agaaagaaag gattcgttta aagagaattt tttatttttc 1860aaagagaaaa ttttaccatc aaaaaaagat acttgtgttt ttaatgaaag acaaaaagat 1920ctttttgaaa aaagtaatga acatattaaa tgcgtttctt cttttaataa tacatcagat 1980gatatttctt cacattcaag cgtaaataag aaagaacctt tttttgcttt aaaaaataat 2040tccataaggc acataccaaa agaaaataat ataatatata catctgggaa atcttttaat 2100catgtgcagg ataaggaaaa gactgttcta cttaaaaaaa agaaagaaat aaatgataag 2160aatacattta gctcttgttt aataaaccat aatataacaa catatacatt acaaaatgga 2220gtaaataaaa atttaaatat gttaggaata agagattcta tttataaaat agatgaaaag 2280aacaatatgt tgaaagaatg ttataatgga aataatgata gtaataataa gaaaaaaaag 2340aagaaaaaaa aattatcttt ttcatgtgat attataaatg ataatattac accttatgaa 2400tcagataagg agaaaaacaa ttctaataat attaagagta tggatatatt taattatgta 2460aaaagaaaaa gcaaccttta taataattta tcttcgaaca gggattctac tgtagatatg 2520cataataaat ataatagtga agaatatata aatatacaga gaacaaataa aatatatgaa 2580ttgagtaata aaagaattag aaattataaa ttgtatagta tggatgaaat atttaaggtg 2640tctcttaagg aaaaaaaata tatagataat atttctaata atatggaaag agtaacatat 2700aaaaatgaaa tgataaatga aaagataagt aagatggacg atatattata tccttgtgac 2760aaaaataaat ctttaaacat gtcttgtcct gttataatag aaaataatat atcaagagaa 2820gaaaatgaaa aaaattcaag tgttatatta aataaaaaaa agaatgagaa tatgtttaat 2880tgtgttggaa ggttacattg tcacatgggc aaaatgaata atcaagataa tatatatgac 2940caagggaata taaaaaaaaa tgaagaagaa ataaccaaac atgatgaata tatatcaagg 3000gaagaaaaaa ataaatataa tagtaaatgt attagaaatt ttgatgacta taaatatgaa 3060caagtgttga gttaccatac gttggatgaa gacaaaaaaa aaaatgatat gaacaattta 3120atagatatga ataatgaggc gattattgaa acggtgaatg gtgttattaa taatattata 3180ttggatagaa aagataataa cagtaggaag gatatggaga aagagatgga gaaggagatg 3240gagaagaaga tggagaagga gatggagaag gtgatggaga aggagatgga gaaggtgatg 3300gagaaggagg tggagaaaga attgaaaaat gagatgaaca ataggatgaa caataggatg 3360aacaatgaga tgaaaaatga aataaacatt tataaaaaca atgagatata tgtagataat 3420gataaagaac tagaaattgt aaatgaggag aagaaactta tttacccatt taattacgaa 3480tctgatgtac ataaaaatat gaatatgtca attaatataa ataattgtaa agatgattat 3540aataatatat taaaagaata tgtagataat tcatgtcttg ctcaaaagga agagaatata 3600tttcgacctt tattcaattt aaataagaaa gataaagtat ggaaacgttt taatataaag 3660aataatatta agacaataat acataatgaa gagatgaaaa gaatatatca aactattaat 3720aaaaatgttt ttcctattta taattttaat cgatatgaaa attttttaat aaatcattta 3780acatataatt ttccaaaaaa tgatttattt aaattatcat acaaagtaag tatgaataat 3840ataaggaatt tgtatattgc taataaacat ataaataata attatgatta tatgaataaa 3900ttatataatc aaaatatata tacattaaaa tatcaggtag ctaatataga taatgatcat 3960catatatgta agaaaggggg agggttggat tatataaata tgaatatatc aaaagaatgt 4020aaaaatagga aagacaaaac atatttaaat aaaatatttc attataagaa gaaaaaggat 4080gcacgctttt ttataaatga cgaaattggt tctaatgatt atatgtatga tataaaaaaa 4140aaatatagta acgatgaaaa taattataaa ttaaatgaaa agatgaacat atctatgtca 4200aatgatgaag atatgattcc tacgttaaat agtgaacatg gaaataattt tccaagttgt 4260caaccgaatt tattagaaaa aaaaagtact tatatagatt tgaacttata tgatagtaat 4320tctatggacg attttacaga agaaaaatat aattttgtta ataatgaaaa tgatttattc 4380aatactaaaa gatggaagtt taatttttcc aagggtaaaa atctgtttaa taataaattt 4440tttaatgtat ctaatgagga tggtgtgttt tcttttttta aaaatatgaa tctttttagg 4500gaacttaata aatcaaataa tagcttaaaa ctagagagtg ttaaaaatag taataataat 4560tgtagtaata ataagggtga tgataatatt ggaaatatgg agaatatgaa tacaacaaat 4620gttacaattg cgagtgatga acatatatct acaaagggag atatacacga cgaatcattt 4680tctagagacg ataatgattg tatcctttta aaaattgaag gtagaagtaa aaaatatagt 4740gatataacct tatataatga ggacaaaagt aatttggaga atgacaatga gactattaat 4800gaatatgaaa atgtatgtag taacatagat gttaatgaat gggaagataa ggtaaatggt 4860acatgtaata gtgttggtga taaagagact gaaaagaata atgaaaagaa taatgaaaag 4920aataatgaaa agaataatga aaagaataat gaaaagaata atgaaaagaa taatgaaaag 4980aataatgaaa agaataatga agaaaataat gaaggaaata atgaagaaaa taatgaagaa 5040aataatgaag aaaataatga agaaaataat gatatagaaa agaatgatat aaaggataat 5100aattcgggac aagtgaaaga aaatataatt gttatgaata atacaaacaa tatggatgtt 5160gataatgatg ataataacaa taattacaat aatgttagta ctgatgaagg tatagatata 5220attaaaaaca tcaaaagtga gatgaatgat tatatttata atgataatat tatgattaag 5280ataaataata aaagtataga tcttatgaat ataaaaaatc aaaagaatga acctttttta 5340aattatacaa atgaaaagga tatacatatg aagagcaatt catcatataa tgtaaatgat 5400aaaatgaatt tatttaataa taatgagaaa acagaaaaaa ataatactag tttaaacgat 5460ttattatata aaagaaaaga agaattagat gatgaaaaaa tatctgaata taaggataca 5520aatttaacaa acaatacctt tgaacatata gctaaaagga ttaatttaat tttgaatgat 5580acaattgaat tttttcaaaa acatacatat cttcataatg gttatggtaa tgttcaggtg 5640tgcaaaaaga acaagaggaa attagaaaaa aagaagttga aaaaatggtc ctgtatttat 5700aaaattaata aaattgtacg taaaggggcc cacggggtgg tgttttctgc gtggagaagt 5760gagaatgttg atttttttaa tcattcgttt tttgaaaact taaatttgga gaataaaaag 5820aagggatata tcgatgaaac aaatgttaat gaaaattatg agtctgataa tgaatatgat 5880agtgatgaag atgatactga aagtgataat gatgatgagc aaaataaaga gaatgaaaga 5940ggtgatgaaa aggatggata tgaagaaatg aatgggggag ataagaatga agaaatgaat 6000gggggagata agaatgaaga aatgaatgtg ggagataaaa atggaggaat aaatgaggaa 6060cataaaaatg aaggaataaa tgaggaacat aaggatgaac taataaataa ggaacataaa 6120aacgagcgaa taaatgagga acataaaaac gaacgaataa atgaggaaca taaaaatgaa 6180ggaataaatg aggaacataa aaatgaagga ataaatgagg aacataaaaa cgaacgaata 6240aatgaggaac ataaaaatga aggaataaat aaactgacct atcataatat gaataaaaat 6300aatatttcaa atgaaaataa ttataatgat gacgattctt atgatgaaga taatttggta 6360tccctgaaga taataaactt aaaatattta agtaaaaaga atagtttaaa aaacattttg 6420agagaagtaa attttttaaa aatgtgtgaa catccaaatg tagtaaaata tttcgaatct 6480tttttttggc ctccttgtta tttagttatt gtgtgtgaat atttatcagg aggaacatta 6540tatgatttat ataaaaatta tggtagaata tcagaagatc ttttagtata tatcttagat 6600gatgtattaa atggtttaaa ttatttacat aatgaatgta gttcaccact tatacataga 6660gatataaaac caacaaatat cgttctttcc aaagatggta tagctaagat aattgatttt 6720ggttcttgtg aagaattgaa aaatagtgat cagtctaaag aattagtggg tactatatat 6780tatatatcac ctgaaatatt gatgagaact aattatgatt gttcatctga tatatggtca 6840ttgggtatta caatatatga aattgtttta tgtaccttac catggaaaag aaatcaatca 6900tttgaaaatt atataaaaac cataattaat tcatcaccaa aaattaacat aacagaagga 6960tatagtaaac acttatgtta ttttgttgag aagtgtttac aaaagaaacc tgagaacaga 7020ggaaatgtga aagatttatt aaatcataaa tttttgatta aaaagaggta tattaaaaag 7080aaacctagtt ctatatatga aataagagat atattaaaaa tatataatgg taaaggtaaa 7140acaaatatct tccgaaattt ttttaagaac ctttttttct tcaatgataa gaataaaaaa 7200aaaaaaccaa ataaaatgat cagttccaaa tcctgtgatg cagaaatgtt ctttgaacag 7260ttaaaaaggg aaaattttga tttttttgaa attaaattaa aagatgatga aaatagtaga 7320tccttgaata cgtttaatat aaatatatct aaagaaagag acgacatatc atattcttct 7380ttaaatttgg aaaaaatcaa agaacacagt ctcaatatgg tagcatctgt tgtcgggact 7440gaacaatccc agaaatga 7458142485PRTPlasmodium falciparum 14Met Phe Ser Val Glu Leu Glu Asn Arg Ser Gly Tyr Lys Lys Arg Lys 1 5 10 15 Lys Lys Lys Trp Asn Asn Lys Ser Thr Gly Gln Asp Lys Phe Thr Asn 20 25 30 Lys Asp Ile Ile Ser Glu Glu Lys Glu Glu Gly Leu Asp Ile Glu Cys 35 40 45 Gly His Asn Ile Leu Gly Asp Val Gln Tyr Asp Gly Thr Tyr Asn Ile 50 55 60 Asn Glu Gln Val Lys Lys Asn Ser Leu Phe Tyr Phe Lys Cys Lys Glu 65 70 75 80 Glu Ile Asn Leu Lys Asp Gly Asn Ile Ile Leu Asp Asp Lys Asn Arg 85 90 95 Lys Val Asp Asp Ile Asn Ile Thr Gly Asp Asp Lys Asn Ile Lys Val 100 105 110 Asp Asp Lys Asn Ile Lys Val Asp Asp Lys Asn Ile Thr Gly Glu Asp 115 120 125 Lys Asn Ile Thr Gly Glu Asp Lys Asn Ile Thr Gly Asp Asp Lys Asn 130 135 140 Ile Ile Phe Asp Val Asp Glu Ile Leu Ile His Gln His Asn Thr Ser 145 150 155 160 Asn Ser Asn Ile Tyr Ile Asn Cys Asn Asp Asn Asn Asn Asp Ile Arg 165 170 175 Asn Ser Ser Asn Val Gln His Tyr Tyr Asn Asp Lys Ile Lys Glu Asn 180 185 190 Ile Asn Lys Gln Asn Lys Lys Tyr Val Leu Ile Asn Asp Tyr Ile Asn 195 200 205 Asn Lys Tyr Ile Leu Ser Lys Asn Lys Thr Cys Lys Ile Asn Lys Gly 210 215 220 Lys Lys Leu Ile Lys Lys Lys Lys Val Asn Asn Ile Ser Arg Arg Arg 225 230 235 240 Asn His Ile Leu Tyr Lys Cys Arg Asn Lys Leu Tyr Asn Gly Asn Val 245 250 255 Phe Ser Asp Asp Ile Ile Lys Ser Glu Val Asn Val Cys Asn Ser Leu 260 265 270 Thr Val Leu His Lys Asn Tyr Asn Ile Asn Met Asp Asn Tyr Leu Asp 275 280 285 Asp Asn Ile His Thr Asn Asn Ser Asn Ile Tyr Asp Ile Asn Tyr Thr 290 295 300 Asn Glu Asn Val Ile Asn Ser Thr Cys Arg Tyr Tyr Pro Ile Gly Asn 305 310 315 320 Asn Asn Thr Leu Ser Lys Asp Glu Val Thr Lys Ser Ser Ser Lys Ile 325 330 335 Asn Ser Leu Ser Tyr Phe Asp Asp Ile Ile Asn Val Asn Lys Asn Asp 340 345 350 Ile Pro Ile Leu His Asp Lys Glu Asn Ile Asn Ile Ile Ser Asn Lys 355 360 365 Glu Ser Cys His Lys Asp Glu Lys Glu Glu Glu Lys Tyr Ile Met Tyr 370 375 380 Asn Ser Asn Leu Val Glu Glu Lys Lys Gln Lys Lys Met Ile Trp Asn 385 390 395 400 Ser Leu Asn Val Leu Pro Ile Asp Ile Leu Leu Lys Asn Gly His Asp 405 410 415 Glu Ile Asn Lys Glu Ile Cys Lys Lys Lys Lys Lys Ser Phe Phe Ser 420 425 430 Gln Asn Asp Ile Lys Ser Lys Met Leu Tyr Asn Asn Lys Ser Tyr Ser 435 440 445 Lys Ser Glu Lys Val Leu Tyr Thr Asn Asn Lys Asn Ser Asn Thr Phe 450 455 460 Ile Pro Ile Phe Phe Leu Asn Lys Val Gly Asp Lys Phe Lys Asn Ser 465 470 475 480 Glu Asn Ile Tyr Asp Met Tyr Asn Asn Lys Lys Asn Val Tyr Ile His 485 490 495 Asp Lys Lys Ile Tyr Thr Asn Met Tyr Ser Asn Lys Leu Lys Gln Lys 500 505 510 His Tyr Tyr Ser Thr Ser Asn Ile Asn Leu Leu Tyr Asn Asn Ile Gly 515 520 525 Lys Val Leu Asp Asn Gly Leu His Leu Ser Asn Asn Met Tyr Cys Arg 530 535 540 Leu Asn Ser Asn Pro Pro Tyr Lys Ser Ile Ser Leu Ile Asn Asn Asn 545 550 555 560 Val Phe Phe Tyr Lys Lys Arg Lys Ser Asn Ser Asn Asn Asn Asn Asn 565 570 575 Asn Asn Asn Ile Ser Ser Ser Ser Ser Ser Ser Ser Lys Lys Asn His 580 585 590 Val Ile Ile Asn Lys Lys Ile Ser Ser Tyr Asn Ile His Tyr Lys Glu 595 600 605 Arg Lys Asp Ser Phe Lys Glu Asn Phe Leu Phe Phe Lys Glu Lys Ile 610 615 620 Leu Pro Ser Lys Lys Asp Thr Cys Val Phe Asn Glu Arg Gln Lys Asp 625 630 635 640 Leu Phe Glu Lys Ser Asn Glu His Ile Lys Cys Val Ser Ser Phe Asn 645 650 655 Asn Thr Ser Asp Asp Ile Ser Ser His Ser Ser Val Asn Lys Lys Glu 660 665 670 Pro Phe Phe Ala Leu Lys Asn Asn Ser Ile Arg His Ile Pro Lys Glu 675 680 685 Asn Asn Ile Ile Tyr Thr Ser Gly Lys Ser Phe Asn His Val Gln Asp 690 695 700 Lys Glu Lys Thr Val Leu Leu Lys Lys Lys Lys Glu Ile Asn Asp Lys 705 710 715 720 Asn Thr Phe Ser Ser Cys Leu Ile Asn His Asn Ile Thr Thr Tyr Thr 725 730 735 Leu Gln Asn Gly Val Asn Lys Asn Leu Asn Met Leu Gly Ile Arg Asp 740 745 750 Ser Ile Tyr Lys Ile Asp Glu Lys Asn Asn Met Leu Lys Glu Cys Tyr 755 760 765 Asn Gly Asn Asn Asp Ser Asn Asn Lys Lys Lys Lys Lys Lys Lys Lys 770 775 780 Leu Ser Phe Ser Cys Asp Ile Ile Asn Asp Asn Ile Thr Pro Tyr Glu 785 790 795 800 Ser Asp Lys Glu Lys Asn Asn Ser Asn Asn Ile Lys Ser Met Asp Ile 805 810 815 Phe Asn Tyr Val Lys Arg Lys Ser Asn Leu Tyr Asn Asn Leu Ser Ser 820 825 830 Asn Arg Asp Ser Thr Val Asp Met His Asn Lys Tyr Asn Ser Glu Glu 835 840 845 Tyr Ile Asn Ile Gln Arg Thr Asn Lys Ile Tyr Glu Leu Ser Asn Lys 850 855 860 Arg Ile Arg Asn Tyr Lys Leu Tyr Ser Met Asp Glu Ile Phe Lys Val 865 870 875 880 Ser Leu Lys Glu Lys Lys Tyr Ile Asp Asn Ile Ser Asn Asn Met Glu 885 890 895 Arg Val Thr Tyr Lys Asn Glu Met Ile Asn Glu Lys Ile Ser Lys Met 900 905 910 Asp Asp Ile Leu Tyr Pro Cys Asp Lys Asn Lys Ser Leu Asn Met Ser 915 920 925 Cys Pro Val Ile Ile Glu Asn Asn Ile Ser Arg Glu Glu Asn Glu Lys 930 935 940 Asn Ser Ser Val Ile Leu Asn Lys Lys Lys Asn Glu Asn Met Phe Asn 945 950 955 960 Cys Val Gly Arg Leu His Cys His Met Gly Lys Met Asn Asn Gln Asp 965 970 975 Asn Ile Tyr Asp Gln Gly Asn Ile Lys Lys Asn Glu Glu Glu Ile Thr 980 985 990 Lys His Asp Glu Tyr Ile Ser Arg Glu Glu Lys Asn Lys Tyr Asn Ser 995 1000 1005 Lys Cys Ile Arg Asn Phe Asp Asp Tyr Lys Tyr Glu Gln Val Leu 1010 1015 1020 Ser Tyr His Thr Leu Asp Glu Asp Lys Lys Lys Asn Asp Met Asn 1025 1030 1035 Asn Leu Ile Asp Met Asn Asn Glu Ala Ile Ile Glu Thr Val Asn 1040 1045 1050 Gly Val Ile Asn Asn Ile Ile Leu Asp Arg Lys Asp Asn Asn Ser 1055 1060 1065 Arg Lys Asp Met Glu Lys Glu Met Glu Lys Glu Met Glu Lys Lys 1070 1075 1080 Met Glu Lys Glu Met Glu Lys Val Met Glu Lys Glu Met Glu Lys 1085 1090 1095 Val Met Glu Lys Glu Val Glu Lys Glu Leu Lys Asn Glu Met Asn 1100 1105 1110 Asn Arg Met Asn Asn

Arg Met Asn Asn Glu Met Lys Asn Glu Ile 1115 1120 1125 Asn Ile Tyr Lys Asn Asn Glu Ile Tyr Val Asp Asn Asp Lys Glu 1130 1135 1140 Leu Glu Ile Val Asn Glu Glu Lys Lys Leu Ile Tyr Pro Phe Asn 1145 1150 1155 Tyr Glu Ser Asp Val His Lys Asn Met Asn Met Ser Ile Asn Ile 1160 1165 1170 Asn Asn Cys Lys Asp Asp Tyr Asn Asn Ile Leu Lys Glu Tyr Val 1175 1180 1185 Asp Asn Ser Cys Leu Ala Gln Lys Glu Glu Asn Ile Phe Arg Pro 1190 1195 1200 Leu Phe Asn Leu Asn Lys Lys Asp Lys Val Trp Lys Arg Phe Asn 1205 1210 1215 Ile Lys Asn Asn Ile Lys Thr Ile Ile His Asn Glu Glu Met Lys 1220 1225 1230 Arg Ile Tyr Gln Thr Ile Asn Lys Asn Val Phe Pro Ile Tyr Asn 1235 1240 1245 Phe Asn Arg Tyr Glu Asn Phe Leu Ile Asn His Leu Thr Tyr Asn 1250 1255 1260 Phe Pro Lys Asn Asp Leu Phe Lys Leu Ser Tyr Lys Val Ser Met 1265 1270 1275 Asn Asn Ile Arg Asn Leu Tyr Ile Ala Asn Lys His Ile Asn Asn 1280 1285 1290 Asn Tyr Asp Tyr Met Asn Lys Leu Tyr Asn Gln Asn Ile Tyr Thr 1295 1300 1305 Leu Lys Tyr Gln Val Ala Asn Ile Asp Asn Asp His His Ile Cys 1310 1315 1320 Lys Lys Gly Gly Gly Leu Asp Tyr Ile Asn Met Asn Ile Ser Lys 1325 1330 1335 Glu Cys Lys Asn Arg Lys Asp Lys Thr Tyr Leu Asn Lys Ile Phe 1340 1345 1350 His Tyr Lys Lys Lys Lys Asp Ala Arg Phe Phe Ile Asn Asp Glu 1355 1360 1365 Ile Gly Ser Asn Asp Tyr Met Tyr Asp Ile Lys Lys Lys Tyr Ser 1370 1375 1380 Asn Asp Glu Asn Asn Tyr Lys Leu Asn Glu Lys Met Asn Ile Ser 1385 1390 1395 Met Ser Asn Asp Glu Asp Met Ile Pro Thr Leu Asn Ser Glu His 1400 1405 1410 Gly Asn Asn Phe Pro Ser Cys Gln Pro Asn Leu Leu Glu Lys Lys 1415 1420 1425 Ser Thr Tyr Ile Asp Leu Asn Leu Tyr Asp Ser Asn Ser Met Asp 1430 1435 1440 Asp Phe Thr Glu Glu Lys Tyr Asn Phe Val Asn Asn Glu Asn Asp 1445 1450 1455 Leu Phe Asn Thr Lys Arg Trp Lys Phe Asn Phe Ser Lys Gly Lys 1460 1465 1470 Asn Leu Phe Asn Asn Lys Phe Phe Asn Val Ser Asn Glu Asp Gly 1475 1480 1485 Val Phe Ser Phe Phe Lys Asn Met Asn Leu Phe Arg Glu Leu Asn 1490 1495 1500 Lys Ser Asn Asn Ser Leu Lys Leu Glu Ser Val Lys Asn Ser Asn 1505 1510 1515 Asn Asn Cys Ser Asn Asn Lys Gly Asp Asp Asn Ile Gly Asn Met 1520 1525 1530 Glu Asn Met Asn Thr Thr Asn Val Thr Ile Ala Ser Asp Glu His 1535 1540 1545 Ile Ser Thr Lys Gly Asp Ile His Asp Glu Ser Phe Ser Arg Asp 1550 1555 1560 Asp Asn Asp Cys Ile Leu Leu Lys Ile Glu Gly Arg Ser Lys Lys 1565 1570 1575 Tyr Ser Asp Ile Thr Leu Tyr Asn Glu Asp Lys Ser Asn Leu Glu 1580 1585 1590 Asn Asp Asn Glu Thr Ile Asn Glu Tyr Glu Asn Val Cys Ser Asn 1595 1600 1605 Ile Asp Val Asn Glu Trp Glu Asp Lys Val Asn Gly Thr Cys Asn 1610 1615 1620 Ser Val Gly Asp Lys Glu Thr Glu Lys Asn Asn Glu Lys Asn Asn 1625 1630 1635 Glu Lys Asn Asn Glu Lys Asn Asn Glu Lys Asn Asn Glu Lys Asn 1640 1645 1650 Asn Glu Lys Asn Asn Glu Lys Asn Asn Glu Lys Asn Asn Glu Glu 1655 1660 1665 Asn Asn Glu Gly Asn Asn Glu Glu Asn Asn Glu Glu Asn Asn Glu 1670 1675 1680 Glu Asn Asn Glu Glu Asn Asn Asp Ile Glu Lys Asn Asp Ile Lys 1685 1690 1695 Asp Asn Asn Ser Gly Gln Val Lys Glu Asn Ile Ile Val Met Asn 1700 1705 1710 Asn Thr Asn Asn Met Asp Val Asp Asn Asp Asp Asn Asn Asn Asn 1715 1720 1725 Tyr Asn Asn Val Ser Thr Asp Glu Gly Ile Asp Ile Ile Lys Asn 1730 1735 1740 Ile Lys Ser Glu Met Asn Asp Tyr Ile Tyr Asn Asp Asn Ile Met 1745 1750 1755 Ile Lys Ile Asn Asn Lys Ser Ile Asp Leu Met Asn Ile Lys Asn 1760 1765 1770 Gln Lys Asn Glu Pro Phe Leu Asn Tyr Thr Asn Glu Lys Asp Ile 1775 1780 1785 His Met Lys Ser Asn Ser Ser Tyr Asn Val Asn Asp Lys Met Asn 1790 1795 1800 Leu Phe Asn Asn Asn Glu Lys Thr Glu Lys Asn Asn Thr Ser Leu 1805 1810 1815 Asn Asp Leu Leu Tyr Lys Arg Lys Glu Glu Leu Asp Asp Glu Lys 1820 1825 1830 Ile Ser Glu Tyr Lys Asp Thr Asn Leu Thr Asn Asn Thr Phe Glu 1835 1840 1845 His Ile Ala Lys Arg Ile Asn Leu Ile Leu Asn Asp Thr Ile Glu 1850 1855 1860 Phe Phe Gln Lys His Thr Tyr Leu His Asn Gly Tyr Gly Asn Val 1865 1870 1875 Gln Val Cys Lys Lys Asn Lys Arg Lys Leu Glu Lys Lys Lys Leu 1880 1885 1890 Lys Lys Trp Ser Cys Ile Tyr Lys Ile Asn Lys Ile Val Arg Lys 1895 1900 1905 Gly Ala His Gly Val Val Phe Ser Ala Trp Arg Ser Glu Asn Val 1910 1915 1920 Asp Phe Phe Asn His Ser Phe Phe Glu Asn Leu Asn Leu Glu Asn 1925 1930 1935 Lys Lys Lys Gly Tyr Ile Asp Glu Thr Asn Val Asn Glu Asn Tyr 1940 1945 1950 Glu Ser Asp Asn Glu Tyr Asp Ser Asp Glu Asp Asp Thr Glu Ser 1955 1960 1965 Asp Asn Asp Asp Glu Gln Asn Lys Glu Asn Glu Arg Gly Asp Glu 1970 1975 1980 Lys Asp Gly Tyr Glu Glu Met Asn Gly Gly Asp Lys Asn Glu Glu 1985 1990 1995 Met Asn Gly Gly Asp Lys Asn Glu Glu Met Asn Val Gly Asp Lys 2000 2005 2010 Asn Gly Gly Ile Asn Glu Glu His Lys Asn Glu Gly Ile Asn Glu 2015 2020 2025 Glu His Lys Asp Glu Leu Ile Asn Lys Glu His Lys Asn Glu Arg 2030 2035 2040 Ile Asn Glu Glu His Lys Asn Glu Arg Ile Asn Glu Glu His Lys 2045 2050 2055 Asn Glu Gly Ile Asn Glu Glu His Lys Asn Glu Gly Ile Asn Glu 2060 2065 2070 Glu His Lys Asn Glu Arg Ile Asn Glu Glu His Lys Asn Glu Gly 2075 2080 2085 Ile Asn Lys Leu Thr Tyr His Asn Met Asn Lys Asn Asn Ile Ser 2090 2095 2100 Asn Glu Asn Asn Tyr Asn Asp Asp Asp Ser Tyr Asp Glu Asp Asn 2105 2110 2115 Leu Val Ser Leu Lys Ile Ile Asn Leu Lys Tyr Leu Ser Lys Lys 2120 2125 2130 Asn Ser Leu Lys Asn Ile Leu Arg Glu Val Asn Phe Leu Lys Met 2135 2140 2145 Cys Glu His Pro Asn Val Val Lys Tyr Phe Glu Ser Phe Phe Trp 2150 2155 2160 Pro Pro Cys Tyr Leu Val Ile Val Cys Glu Tyr Leu Ser Gly Gly 2165 2170 2175 Thr Leu Tyr Asp Leu Tyr Lys Asn Tyr Gly Arg Ile Ser Glu Asp 2180 2185 2190 Leu Leu Val Tyr Ile Leu Asp Asp Val Leu Asn Gly Leu Asn Tyr 2195 2200 2205 Leu His Asn Glu Cys Ser Ser Pro Leu Ile His Arg Asp Ile Lys 2210 2215 2220 Pro Thr Asn Ile Val Leu Ser Lys Asp Gly Ile Ala Lys Ile Ile 2225 2230 2235 Asp Phe Gly Ser Cys Glu Glu Leu Lys Asn Ser Asp Gln Ser Lys 2240 2245 2250 Glu Leu Val Gly Thr Ile Tyr Tyr Ile Ser Pro Glu Ile Leu Met 2255 2260 2265 Arg Thr Asn Tyr Asp Cys Ser Ser Asp Ile Trp Ser Leu Gly Ile 2270 2275 2280 Thr Ile Tyr Glu Ile Val Leu Cys Thr Leu Pro Trp Lys Arg Asn 2285 2290 2295 Gln Ser Phe Glu Asn Tyr Ile Lys Thr Ile Ile Asn Ser Ser Pro 2300 2305 2310 Lys Ile Asn Ile Thr Glu Gly Tyr Ser Lys His Leu Cys Tyr Phe 2315 2320 2325 Val Glu Lys Cys Leu Gln Lys Lys Pro Glu Asn Arg Gly Asn Val 2330 2335 2340 Lys Asp Leu Leu Asn His Lys Phe Leu Ile Lys Lys Arg Tyr Ile 2345 2350 2355 Lys Lys Lys Pro Ser Ser Ile Tyr Glu Ile Arg Asp Ile Leu Lys 2360 2365 2370 Ile Tyr Asn Gly Lys Gly Lys Thr Asn Ile Phe Arg Asn Phe Phe 2375 2380 2385 Lys Asn Leu Phe Phe Phe Asn Asp Lys Asn Lys Lys Lys Lys Pro 2390 2395 2400 Asn Lys Met Ile Ser Ser Lys Ser Cys Asp Ala Glu Met Phe Phe 2405 2410 2415 Glu Gln Leu Lys Arg Glu Asn Phe Asp Phe Phe Glu Ile Lys Leu 2420 2425 2430 Lys Asp Asp Glu Asn Ser Arg Ser Leu Asn Thr Phe Asn Ile Asn 2435 2440 2445 Ile Ser Lys Glu Arg Asp Asp Ile Ser Tyr Ser Ser Leu Asn Leu 2450 2455 2460 Glu Lys Ile Lys Glu His Ser Leu Asn Met Val Ala Ser Val Val 2465 2470 2475 Gly Thr Glu Gln Ser Gln Lys 2480 2485 151869DNAPlasmodium falciparum 15atgagaaaat tatactgcgt attattattg agcgcctttg agtttacata tatgataaac 60tttggaagag gacagaatta ttgggaacat ccatatcaaa atagtgatgt gtatcgtcca 120atcaacgaac atagggaaca tccaaaagaa tacgaatatc cattacacca ggaacataca 180taccaacaag aagattcagg agaagacgaa aatacattac aacacgcata tccaatagac 240cacgaaggtg ccgaacccgc accacaagaa caaaatttat tttcaagcat tgaaatagta 300gaaagaagta attatatggg taatccatgg acggaatata tggcaaaata tgatattgaa 360gaagttcatg gttcaggtat aagagtagat ttaggagaag atgctgaagt agctggaact 420caatatagac ttccatcagg gaaatgtcca gtatttggta aaggtataat tattgagaat 480tcaaatacta cttttttaac accggtagct acgggaaatc aatatttaaa agatggaggt 540tttgcttttc ctccaacaga acctcttatg tcaccaatga cattagatga aatgagacat 600ttttataaag ataataaata tgtaaaaaat ttagatgaat tgactttatg ttcaagacat 660gcaggaaata tgattccaga taatgataaa aattcaaatt ataaatatcc agctgtttat 720gatgacaaag ataaaaagtg tcatatatta tatattgcag ctcaagaaaa taatggtcct 780agatattgta ataaagacga aagtaaaaga aacagcatgt tttgttttag accagcaaaa 840gatatatcat ttcaaaacta tacatattta agtaagaatg tagttgataa ctgggaaaaa 900gtttgcccta gaaagaattt acagaatgca aaattcggat tatgggtcga tggaaattgt 960gaagatatac cacatgtaaa tgaatttcca gcaattgatc tttttgaatg taataaatta 1020gtttttgaat tgagtgcttc ggatcaacct aaacaatatg aacaacattt aacagattat 1080gaaaaaatta aagaaggttt caaaaataag aacgctagta tgatcaaaag tgcttttctt 1140cccactggtg cttttaaagc agatagatat aaaagtcatg gtaagggtta taattgggga 1200aattataaca cagaaacaca aaaatgtgaa atttttaatg tcaaaccaac atgtttaatt 1260aacaattcat catacattgc tactactgct ttgtcccatc ccatcgaagt tgaaaacaat 1320tttccatgtt cattatataa agatgaaata atgaaagaaa tcgaaagaga atcaaaacga 1380attaaattaa atgataatga tgatgaaggg aataaaaaaa ttatagctcc aagaattttt 1440atttcagatg ataaagacag tttaaaatgc ccatgtgacc ctgaaatggt aagtaatagt 1500acatgtcgtt tctttgtatg taaatgtgta gaaagaaggg cagaagtaac atcaaataat 1560gaagttgtag ttaaagaaga atataaagat gaatatgcag atattcctga acataaacca 1620acttatgata aaatgaaaat tataattgca tcatcagctg ctgtcgctgt attagcaact 1680attttaatgg tttatcttta taaaagaaaa ggaaatgctg aaaaatatga taaaatggat 1740gaaccacaag attatgggaa atcaaattca agaaatgatg aaatgttaga tcctgaggca 1800tctttttggg gggaagaaaa aagagcatca catacaacac cagttctgat ggaaaaacca 1860tactattaa 186916622PRTPlasmodium falciparum 16Met Arg Lys Leu Tyr Cys Val Leu Leu Leu Ser Ala Phe Glu Phe Thr 1 5 10 15 Tyr Met Ile Asn Phe Gly Arg Gly Gln Asn Tyr Trp Glu His Pro Tyr 20 25 30 Gln Asn Ser Asp Val Tyr Arg Pro Ile Asn Glu His Arg Glu His Pro 35 40 45 Lys Glu Tyr Glu Tyr Pro Leu His Gln Glu His Thr Tyr Gln Gln Glu 50 55 60 Asp Ser Gly Glu Asp Glu Asn Thr Leu Gln His Ala Tyr Pro Ile Asp 65 70 75 80 His Glu Gly Ala Glu Pro Ala Pro Gln Glu Gln Asn Leu Phe Ser Ser 85 90 95 Ile Glu Ile Val Glu Arg Ser Asn Tyr Met Gly Asn Pro Trp Thr Glu 100 105 110 Tyr Met Ala Lys Tyr Asp Ile Glu Glu Val His Gly Ser Gly Ile Arg 115 120 125 Val Asp Leu Gly Glu Asp Ala Glu Val Ala Gly Thr Gln Tyr Arg Leu 130 135 140 Pro Ser Gly Lys Cys Pro Val Phe Gly Lys Gly Ile Ile Ile Glu Asn 145 150 155 160 Ser Asn Thr Thr Phe Leu Thr Pro Val Ala Thr Gly Asn Gln Tyr Leu 165 170 175 Lys Asp Gly Gly Phe Ala Phe Pro Pro Thr Glu Pro Leu Met Ser Pro 180 185 190 Met Thr Leu Asp Glu Met Arg His Phe Tyr Lys Asp Asn Lys Tyr Val 195 200 205 Lys Asn Leu Asp Glu Leu Thr Leu Cys Ser Arg His Ala Gly Asn Met 210 215 220 Ile Pro Asp Asn Asp Lys Asn Ser Asn Tyr Lys Tyr Pro Ala Val Tyr 225 230 235 240 Asp Asp Lys Asp Lys Lys Cys His Ile Leu Tyr Ile Ala Ala Gln Glu 245 250 255 Asn Asn Gly Pro Arg Tyr Cys Asn Lys Asp Glu Ser Lys Arg Asn Ser 260 265 270 Met Phe Cys Phe Arg Pro Ala Lys Asp Ile Ser Phe Gln Asn Tyr Thr 275 280 285 Tyr Leu Ser Lys Asn Val Val Asp Asn Trp Glu Lys Val Cys Pro Arg 290 295 300 Lys Asn Leu Gln Asn Ala Lys Phe Gly Leu Trp Val Asp Gly Asn Cys 305 310 315 320 Glu Asp Ile Pro His Val Asn Glu Phe Pro Ala Ile Asp Leu Phe Glu 325 330 335 Cys Asn Lys Leu Val Phe Glu Leu Ser Ala Ser Asp Gln Pro Lys Gln 340 345 350 Tyr Glu Gln His Leu Thr Asp Tyr Glu Lys Ile Lys Glu Gly Phe Lys 355 360 365 Asn Lys Asn Ala Ser Met Ile Lys Ser Ala Phe Leu Pro Thr Gly Ala 370 375 380 Phe Lys Ala Asp Arg Tyr Lys Ser His Gly Lys Gly Tyr Asn Trp Gly 385 390 395 400 Asn Tyr Asn Thr Glu Thr Gln Lys Cys Glu Ile Phe Asn Val Lys Pro 405 410 415 Thr Cys Leu Ile Asn Asn Ser Ser Tyr Ile Ala Thr Thr Ala Leu Ser 420 425 430 His Pro Ile Glu Val Glu Asn Asn Phe Pro Cys Ser Leu Tyr Lys Asp 435 440 445 Glu Ile Met Lys Glu Ile Glu Arg Glu Ser Lys Arg Ile Lys Leu Asn 450 455 460 Asp Asn Asp Asp Glu Gly Asn Lys Lys Ile Ile Ala Pro Arg Ile Phe 465 470 475 480 Ile Ser Asp Asp Lys Asp Ser Leu Lys Cys Pro Cys Asp Pro Glu Met 485 490 495 Val Ser Asn Ser Thr Cys Arg Phe Phe Val Cys Lys Cys Val Glu Arg 500 505 510 Arg Ala Glu Val Thr Ser Asn Asn Glu Val Val Val Lys Glu Glu Tyr 515 520 525 Lys Asp Glu Tyr Ala Asp Ile Pro Glu His Lys Pro Thr Tyr Asp Lys 530 535 540 Met Lys Ile Ile Ile Ala Ser Ser Ala Ala Val Ala Val Leu Ala Thr 545 550 555 560 Ile Leu Met Val Tyr Leu Tyr Lys Arg Lys

Gly Asn Ala Glu Lys Tyr 565 570 575 Asp Lys Met Asp Glu Pro Gln Asp Tyr Gly Lys Ser Asn Ser Arg Asn 580 585 590 Asp Glu Met Leu Asp Pro Glu Ala Ser Phe Trp Gly Glu Glu Lys Arg 595 600 605 Ala Ser His Thr Thr Pro Val Leu Met Glu Lys Pro Tyr Tyr 610 615 620 171227DNAPlasmodium falciparum 17atgttactct tttttgcaaa acttgtcgta tttacctttt tcttttggct tttaaaatat 60gggaaaacga ggtcatatcc caaatctggc cataagggac atacgaaatt aaatcaacca 120gtagttagaa cattagcaga ttttaatgac atgtttgcaa accaaaaaaa tacatttaat 180tttctaaaac atataaatca ttataaaaat gaacaagata caaataatac acacacgcca 240aatcatgatg aatattctca taatttgcca aaaaatcacg aagagtcaaa tgcaaatatg 300aacaatcata attctttcaa tgacaaatct gttaataaaa aagaagcttt cgatcaattt 360ttacaaacgt tattaaacaa ttatgaaata atgcataaag aagatgaaag taaagaatca 420aatcaacata actataaaga aggtccctca tatgaagata aaaaaaatat gtacaaagaa 480atattgaaag gatattataa tgtatttttt gaaaattatg caaacgacac agaatcaaat 540gtacataata aacctgagga agttcataaa catgaggaaa ttcataaaca taggaaactt 600cataaacatg aagaagttca taaacctgag gaatttcata aacctgagga atttcataaa 660catgagaaag ttcataaaca tgaagaagtt cataaacctg aggaagttca taaacatgag 720gaaaatcata aacatgagga aaatcataaa cctcaaatgg taggtcaagc acctccagaa 780aaagagatac gccaagaatc aagaactcta atacttggtt catttcccca agcaggtgaa 840atattaagag aggatttatg gaacaaagag gataacaaat ttagttacgc acttgaccct 900aatgattatg catctataga agataaactt ttaggatcta tatttggata ctttaaaaaa 960aatcatgaca atttggttaa acatttgtta caacaaatta atacttacaa acataaatat 1020atggaactta aagaacaata tattaatgaa gttatgaaac ttaaaaaaat atataacaaa 1080agcatcatgg tcatatttat agcatcttgt atttcaatat taggacctgt aatgttacac 1140atgcatcaaa ataatccaga agaatttttt gcgaccatat taagtttttc tatatcatta 1200ggtcttcata atttattact aacttaa 122718408PRTPlasmodium falciparum 18Met Leu Leu Phe Phe Ala Lys Leu Val Val Phe Thr Phe Phe Phe Trp 1 5 10 15 Leu Leu Lys Tyr Gly Lys Thr Arg Ser Tyr Pro Lys Ser Gly His Lys 20 25 30 Gly His Thr Lys Leu Asn Gln Pro Val Val Arg Thr Leu Ala Asp Phe 35 40 45 Asn Asp Met Phe Ala Asn Gln Lys Asn Thr Phe Asn Phe Leu Lys His 50 55 60 Ile Asn His Tyr Lys Asn Glu Gln Asp Thr Asn Asn Thr His Thr Pro 65 70 75 80 Asn His Asp Glu Tyr Ser His Asn Leu Pro Lys Asn His Glu Glu Ser 85 90 95 Asn Ala Asn Met Asn Asn His Asn Ser Phe Asn Asp Lys Ser Val Asn 100 105 110 Lys Lys Glu Ala Phe Asp Gln Phe Leu Gln Thr Leu Leu Asn Asn Tyr 115 120 125 Glu Ile Met His Lys Glu Asp Glu Ser Lys Glu Ser Asn Gln His Asn 130 135 140 Tyr Lys Glu Gly Pro Ser Tyr Glu Asp Lys Lys Asn Met Tyr Lys Glu 145 150 155 160 Ile Leu Lys Gly Tyr Tyr Asn Val Phe Phe Glu Asn Tyr Ala Asn Asp 165 170 175 Thr Glu Ser Asn Val His Asn Lys Pro Glu Glu Val His Lys His Glu 180 185 190 Glu Ile His Lys His Arg Lys Leu His Lys His Glu Glu Val His Lys 195 200 205 Pro Glu Glu Phe His Lys Pro Glu Glu Phe His Lys His Glu Lys Val 210 215 220 His Lys His Glu Glu Val His Lys Pro Glu Glu Val His Lys His Glu 225 230 235 240 Glu Asn His Lys His Glu Glu Asn His Lys Pro Gln Met Val Gly Gln 245 250 255 Ala Pro Pro Glu Lys Glu Ile Arg Gln Glu Ser Arg Thr Leu Ile Leu 260 265 270 Gly Ser Phe Pro Gln Ala Gly Glu Ile Leu Arg Glu Asp Leu Trp Asn 275 280 285 Lys Glu Asp Asn Lys Phe Ser Tyr Ala Leu Asp Pro Asn Asp Tyr Ala 290 295 300 Ser Ile Glu Asp Lys Leu Leu Gly Ser Ile Phe Gly Tyr Phe Lys Lys 305 310 315 320 Asn His Asp Asn Leu Val Lys His Leu Leu Gln Gln Ile Asn Thr Tyr 325 330 335 Lys His Lys Tyr Met Glu Leu Lys Glu Gln Tyr Ile Asn Glu Val Met 340 345 350 Lys Leu Lys Lys Ile Tyr Asn Lys Ser Ile Met Val Ile Phe Ile Ala 355 360 365 Ser Cys Ile Ser Ile Leu Gly Pro Val Met Leu His Met His Gln Asn 370 375 380 Asn Pro Glu Glu Phe Phe Ala Thr Ile Leu Ser Phe Ser Ile Ser Leu 385 390 395 400 Gly Leu His Asn Leu Leu Leu Thr 405 194398DNAPlasmodium falciparum 19atggattatt tgattaggaa taatgaatac tgtaatgttt tatatgatgg aaatgaatta 60ttaaggaaaa gaaaaataaa ttttgacaat gagatttgtc cttataaaat gaattattgg 120aataattaca acaataacat aaatattgat aatgataata tgaataatga aatagtaaat 180gaagaatttg agaatactta taataatatt aataataata tgtattatct tcttggaaaa 240gaggacatgg acgaacctag attgtgtaat aaaaaaagaa atgtttacaa tgaaaatttt 300aagaataact tagaatatga tgagaatgta aataattatg ataatgataa cagtagtaat 360atatcataca aatttattaa taatgagtgt gacaagatga aaaaatctgg agaaggtact 420caggagaaca atgataatga aaataataat aatgatgaga gtaacaataa taataataat 480aataataatg agagtaataa taataataat aataatgaga gtaacaataa taataataat 540aataataata atgagagtaa caataataat aataataatg agagtaataa taataatcat 600gggaataata ataatagtag taataataac aacaataata ataataataa caacaacaac 660aacaataata ataataataa taataataat aataacaata ataataacaa caataataat 720gataagaatg aagatgatga aaatactaca gggaaaggaa aaaaaacaga agtcaaaaaa 780cgtgcaggga gaaaaagaaa tgattccaca atgtatgcca atagtaatac aatgttaaaa 840aataattttt tacaaaataa taaaatgatt tataataata tggatcatat aaatgataca 900aatgaacaag ataaattttt atatgaacaa agaaaaatga tgatgaataa taaaatggaa 960aatatgggac ccaatatgaa tatgacaaat aaaaattatt ttcaaacagt aaataacatg 1020aaaatgaatg tagaaaataa taaaaatctt ataggtataa atacaaataa tatgaataat 1080tatcaaaata atataaatag tattaatact aatatgaata taccaaagaa caaaaattta 1140cattcaaata aacaaatgat gtttatgaat aataatatga atatacatgg aaaaaccaat 1200aaattaaatg atatggctag aatgaatagt ataaacttta atgaagaaaa taaaatgtct 1260gttatgaatg acgacacaag aatggataat aaaatgaggg atataatgaa aagtggtgga 1320atggaggtac atttaaaaaa tgaagaatcc ttatatatga ataatatgta taatatgaat 1380agtaataatc caaataatat acctagaatt agtacaatga atattgcaaa acctatgaat 1440aattttaata atatgaatat gcctataaat aatatgaatg catataacaa tcacatgatt 1500aattcgtata ataatatgat gatgacaaat aatatgaaca acataaataa tataaataat 1560ataaatcata taaataataa taataatagt aataataata ataatagtaa taatagtaat 1620aataatatta attttggtca gcatattcag ggggtacaat ttatgaatcc taatatgaat 1680aacgacatga acagtaatat gaataatatt aataataatc aaatgttctt gaataataac 1740cagaataatg cacatgttca tatggttaac aacatgaatt tgaatccctc tggaaataat 1800tcatttttta ataattcaaa taaacatctg aatatgatga ccatgagttc aaaaaataaa 1860aaaaacgcca tattaaaaaa tacagaaaat aatgaatttg aaaatataaa aagagtacat 1920tcaaacagct ccaactataa taataataat aataataata gtgatagtaa taataaaaaa 1980tcgattaaaa tgaatagcaa tgaaaacaat aatacaacaa caactattat aaaaaatata 2040cgagttgaaa acataaagtt taatgaaagt gtcaatttaa aaaatattga tggcttagat 2100ctaagtacta attttgttaa tggaaaatat atatccaaat taaataatga tgagaaaata 2160tataatgtag tacattctat tgtgaaagat tctctcaaag attgtttagt tgatttttat 2220aataatgcaa attatatttt taaagataaa ttagattttg tatgtaatag taatactcct 2280acatgtaata atataaatga tttagaagac aacaaaaagg atgacgggca tgtggataaa 2340caaaattatt cacttgaaaa gaaagataat gaagaacaca caaataataa ttctgatcct 2400ataatggatg gtgttgagtt ggatgtaaaa aagatatctg aaaagaagga ggatcaaatt 2460gaaaaggaca aaccaaaaaa tgataataca aaaaatgata atacaaagga agacaaaaat 2520caaaatgatg ataataaaac taatacatca attccaaaaa ctcccaaaaa tgaagttaaa 2580aatgttatta cctcttcctt attgtcaagt tttaatatat ttagtgcttt cagtaatagt 2640aatacaccta attcgaaaaa aaataaatat gatgaagagg aagaaaaaga agaaaaagaa 2700gaagaagaag aaaataataa atgtaatgat aaagatgatg aggataaaaa taagtctcct 2760caaaatgaag ataataaaaa tattaccaat gataataatg ataataataa taataatgta 2820gacaaagaaa acaatttaaa agaatctata gataacctag gaaatgttga tacagataat 2880aatattaaga gagatgtact aaattataat aatatagaag aaaatgaaca aagcagagaa 2940tatattttaa tagaaattac aaaaagtata tgctctataa tcaatctaca acaattaatg 3000cctgttaata ctaggttagc taaccctaat cttatatatg atccaaatta tgaaaccata 3060tattctaaat ggaaaacatt tttaagaaaa gagcaatcaa gtggaaattt aattagtaat 3120tgtttttcac gagatttttt acatactatt ttgttatgta attatgtaac tataattgaa 3180gatttaagaa aaacggccgt taagaaaaag ttgaaatatt ttttcttaca cttatgtttg 3240gaatcgggca tatcgattaa cgttgcattg atgctgttta taaatgccac gaagcagagc 3300gataagttac agtctctact accctccgaa acaggattag gatatttgca cagagatgcg 3360ggtggagcaa aagaagagaa tatgggtatt ataacatttg aatgtataac gaacgatagg 3420gaaccagatc atttaataaa attaataact ttgaaaaata ttttctcaag acagttacca 3480aaaatgccta gagaatatat agtaaggctt gtatttgata gaaatcatta tacattttgt 3540ttattaaaaa aaaatactgt tataggtgga gtatgtttca gaccatattt tgaacagaaa 3600tttgcggaaa ttgcattttt agctgttacc tcaactgagc aggtaaaagg atatggtaca 3660agattaatga atcatttgaa ggagcatgta aagaagtttg gtatagaata tttcttaacc 3720tatgcagata actttgccat aggatatttt agaaaacaag gattttcaca aaaaatttcc 3780atgccaaaag aaagatggtt tggatatatt aaagattatg atggtggtac attaatggaa 3840tgttacattt tcccaaacat caattatttg agactttcgg aaatgttata tgagcaaaaa 3900aaagcagtaa agaaagctat acattttata aaacctcaag ttatatataa gggtattaat 3960tattttgctg ataataaagg ggctgcttta catccaagta ctattcctgg attattagaa 4020gttggctgga aaaaggaaac gagggaaatt actaaaaagg ttcaacataa agaagttcaa 4080ttgaaagatc aaattttagg tgttctggat tatttagaaa aacaacagtc tgcatggcca 4140tttcttaagc cagttagtct ttcagaagct cctgattatt atgatattat aaaagaacca 4200acagatattt tgaccatgag aagaaaggcc agacatggtg attataaaac gaaggaagat 4260tttggtattg agcttaagag aatgtttgat aattgtcgtt tgtataatgc tccaacaact 4320atttatttca aatatgcaaa tgaactacaa acacttatat ggcctaaata tgaagctata 4380actgatacag caaaataa 4398201465PRTPlasmodium falciparum 20Met Asp Tyr Leu Ile Arg Asn Asn Glu Tyr Cys Asn Val Leu Tyr Asp 1 5 10 15 Gly Asn Glu Leu Leu Arg Lys Arg Lys Ile Asn Phe Asp Asn Glu Ile 20 25 30 Cys Pro Tyr Lys Met Asn Tyr Trp Asn Asn Tyr Asn Asn Asn Ile Asn 35 40 45 Ile Asp Asn Asp Asn Met Asn Asn Glu Ile Val Asn Glu Glu Phe Glu 50 55 60 Asn Thr Tyr Asn Asn Ile Asn Asn Asn Met Tyr Tyr Leu Leu Gly Lys 65 70 75 80 Glu Asp Met Asp Glu Pro Arg Leu Cys Asn Lys Lys Arg Asn Val Tyr 85 90 95 Asn Glu Asn Phe Lys Asn Asn Leu Glu Tyr Asp Glu Asn Val Asn Asn 100 105 110 Tyr Asp Asn Asp Asn Ser Ser Asn Ile Ser Tyr Lys Phe Ile Asn Asn 115 120 125 Glu Cys Asp Lys Met Lys Lys Ser Gly Glu Gly Thr Gln Glu Asn Asn 130 135 140 Asp Asn Glu Asn Asn Asn Asn Asp Glu Ser Asn Asn Asn Asn Asn Asn 145 150 155 160 Asn Asn Asn Glu Ser Asn Asn Asn Asn Asn Asn Asn Glu Ser Asn Asn 165 170 175 Asn Asn Asn Asn Asn Asn Asn Asn Glu Ser Asn Asn Asn Asn Asn Asn 180 185 190 Asn Glu Ser Asn Asn Asn Asn His Gly Asn Asn Asn Asn Ser Ser Asn 195 200 205 Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn 210 215 220 Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn 225 230 235 240 Asp Lys Asn Glu Asp Asp Glu Asn Thr Thr Gly Lys Gly Lys Lys Thr 245 250 255 Glu Val Lys Lys Arg Ala Gly Arg Lys Arg Asn Asp Ser Thr Met Tyr 260 265 270 Ala Asn Ser Asn Thr Met Leu Lys Asn Asn Phe Leu Gln Asn Asn Lys 275 280 285 Met Ile Tyr Asn Asn Met Asp His Ile Asn Asp Thr Asn Glu Gln Asp 290 295 300 Lys Phe Leu Tyr Glu Gln Arg Lys Met Met Met Asn Asn Lys Met Glu 305 310 315 320 Asn Met Gly Pro Asn Met Asn Met Thr Asn Lys Asn Tyr Phe Gln Thr 325 330 335 Val Asn Asn Met Lys Met Asn Val Glu Asn Asn Lys Asn Leu Ile Gly 340 345 350 Ile Asn Thr Asn Asn Met Asn Asn Tyr Gln Asn Asn Ile Asn Ser Ile 355 360 365 Asn Thr Asn Met Asn Ile Pro Lys Asn Lys Asn Leu His Ser Asn Lys 370 375 380 Gln Met Met Phe Met Asn Asn Asn Met Asn Ile His Gly Lys Thr Asn 385 390 395 400 Lys Leu Asn Asp Met Ala Arg Met Asn Ser Ile Asn Phe Asn Glu Glu 405 410 415 Asn Lys Met Ser Val Met Asn Asp Asp Thr Arg Met Asp Asn Lys Met 420 425 430 Arg Asp Ile Met Lys Ser Gly Gly Met Glu Val His Leu Lys Asn Glu 435 440 445 Glu Ser Leu Tyr Met Asn Asn Met Tyr Asn Met Asn Ser Asn Asn Pro 450 455 460 Asn Asn Ile Pro Arg Ile Ser Thr Met Asn Ile Ala Lys Pro Met Asn 465 470 475 480 Asn Phe Asn Asn Met Asn Met Pro Ile Asn Asn Met Asn Ala Tyr Asn 485 490 495 Asn His Met Ile Asn Ser Tyr Asn Asn Met Met Met Thr Asn Asn Met 500 505 510 Asn Asn Ile Asn Asn Ile Asn Asn Ile Asn His Ile Asn Asn Asn Asn 515 520 525 Asn Ser Asn Asn Asn Asn Asn Ser Asn Asn Ser Asn Asn Asn Ile Asn 530 535 540 Phe Gly Gln His Ile Gln Gly Val Gln Phe Met Asn Pro Asn Met Asn 545 550 555 560 Asn Asp Met Asn Ser Asn Met Asn Asn Ile Asn Asn Asn Gln Met Phe 565 570 575 Leu Asn Asn Asn Gln Asn Asn Ala His Val His Met Val Asn Asn Met 580 585 590 Asn Leu Asn Pro Ser Gly Asn Asn Ser Phe Phe Asn Asn Ser Asn Lys 595 600 605 His Leu Asn Met Met Thr Met Ser Ser Lys Asn Lys Lys Asn Ala Ile 610 615 620 Leu Lys Asn Thr Glu Asn Asn Glu Phe Glu Asn Ile Lys Arg Val His 625 630 635 640 Ser Asn Ser Ser Asn Tyr Asn Asn Asn Asn Asn Asn Asn Ser Asp Ser 645 650 655 Asn Asn Lys Lys Ser Ile Lys Met Asn Ser Asn Glu Asn Asn Asn Thr 660 665 670 Thr Thr Thr Ile Ile Lys Asn Ile Arg Val Glu Asn Ile Lys Phe Asn 675 680 685 Glu Ser Val Asn Leu Lys Asn Ile Asp Gly Leu Asp Leu Ser Thr Asn 690 695 700 Phe Val Asn Gly Lys Tyr Ile Ser Lys Leu Asn Asn Asp Glu Lys Ile 705 710 715 720 Tyr Asn Val Val His Ser Ile Val Lys Asp Ser Leu Lys Asp Cys Leu 725 730 735 Val Asp Phe Tyr Asn Asn Ala Asn Tyr Ile Phe Lys Asp Lys Leu Asp 740 745 750 Phe Val Cys Asn Ser Asn Thr Pro Thr Cys Asn Asn Ile Asn Asp Leu 755 760 765 Glu Asp Asn Lys Lys Asp Asp Gly His Val Asp Lys Gln Asn Tyr Ser 770 775 780 Leu Glu Lys Lys Asp Asn Glu Glu His Thr Asn Asn Asn Ser Asp Pro 785 790 795 800 Ile Met Asp Gly Val Glu Leu Asp Val Lys Lys Ile Ser Glu Lys Lys 805 810 815 Glu Asp Gln Ile Glu Lys Asp Lys Pro Lys Asn Asp Asn Thr Lys Asn 820 825 830 Asp Asn Thr Lys Glu Asp Lys Asn Gln Asn Asp Asp Asn Lys Thr Asn 835 840 845 Thr Ser Ile Pro Lys Thr Pro Lys Asn Glu Val Lys Asn Val Ile Thr 850 855 860 Ser Ser Leu Leu Ser Ser Phe Asn Ile Phe Ser Ala Phe Ser Asn Ser 865 870 875 880 Asn Thr Pro Asn Ser Lys Lys Asn Lys Tyr Asp Glu Glu Glu Glu Lys 885 890 895 Glu Glu Lys Glu Glu Glu Glu Glu Asn Asn Lys Cys Asn Asp Lys Asp 900 905 910 Asp Glu Asp Lys Asn Lys Ser Pro Gln Asn Glu Asp Asn Lys Asn Ile 915 920 925 Thr Asn Asp Asn Asn Asp Asn Asn Asn Asn Asn Val Asp Lys Glu Asn 930 935 940 Asn Leu Lys Glu Ser Ile Asp Asn Leu Gly Asn Val

Asp Thr Asp Asn 945 950 955 960 Asn Ile Lys Arg Asp Val Leu Asn Tyr Asn Asn Ile Glu Glu Asn Glu 965 970 975 Gln Ser Arg Glu Tyr Ile Leu Ile Glu Ile Thr Lys Ser Ile Cys Ser 980 985 990 Ile Ile Asn Leu Gln Gln Leu Met Pro Val Asn Thr Arg Leu Ala Asn 995 1000 1005 Pro Asn Leu Ile Tyr Asp Pro Asn Tyr Glu Thr Ile Tyr Ser Lys 1010 1015 1020 Trp Lys Thr Phe Leu Arg Lys Glu Gln Ser Ser Gly Asn Leu Ile 1025 1030 1035 Ser Asn Cys Phe Ser Arg Asp Phe Leu His Thr Ile Leu Leu Cys 1040 1045 1050 Asn Tyr Val Thr Ile Ile Glu Asp Leu Arg Lys Thr Ala Val Lys 1055 1060 1065 Lys Lys Leu Lys Tyr Phe Phe Leu His Leu Cys Leu Glu Ser Gly 1070 1075 1080 Ile Ser Ile Asn Val Ala Leu Met Leu Phe Ile Asn Ala Thr Lys 1085 1090 1095 Gln Ser Asp Lys Leu Gln Ser Leu Leu Pro Ser Glu Thr Gly Leu 1100 1105 1110 Gly Tyr Leu His Arg Asp Ala Gly Gly Ala Lys Glu Glu Asn Met 1115 1120 1125 Gly Ile Ile Thr Phe Glu Cys Ile Thr Asn Asp Arg Glu Pro Asp 1130 1135 1140 His Leu Ile Lys Leu Ile Thr Leu Lys Asn Ile Phe Ser Arg Gln 1145 1150 1155 Leu Pro Lys Met Pro Arg Glu Tyr Ile Val Arg Leu Val Phe Asp 1160 1165 1170 Arg Asn His Tyr Thr Phe Cys Leu Leu Lys Lys Asn Thr Val Ile 1175 1180 1185 Gly Gly Val Cys Phe Arg Pro Tyr Phe Glu Gln Lys Phe Ala Glu 1190 1195 1200 Ile Ala Phe Leu Ala Val Thr Ser Thr Glu Gln Val Lys Gly Tyr 1205 1210 1215 Gly Thr Arg Leu Met Asn His Leu Lys Glu His Val Lys Lys Phe 1220 1225 1230 Gly Ile Glu Tyr Phe Leu Thr Tyr Ala Asp Asn Phe Ala Ile Gly 1235 1240 1245 Tyr Phe Arg Lys Gln Gly Phe Ser Gln Lys Ile Ser Met Pro Lys 1250 1255 1260 Glu Arg Trp Phe Gly Tyr Ile Lys Asp Tyr Asp Gly Gly Thr Leu 1265 1270 1275 Met Glu Cys Tyr Ile Phe Pro Asn Ile Asn Tyr Leu Arg Leu Ser 1280 1285 1290 Glu Met Leu Tyr Glu Gln Lys Lys Ala Val Lys Lys Ala Ile His 1295 1300 1305 Phe Ile Lys Pro Gln Val Ile Tyr Lys Gly Ile Asn Tyr Phe Ala 1310 1315 1320 Asp Asn Lys Gly Ala Ala Leu His Pro Ser Thr Ile Pro Gly Leu 1325 1330 1335 Leu Glu Val Gly Trp Lys Lys Glu Thr Arg Glu Ile Thr Lys Lys 1340 1345 1350 Val Gln His Lys Glu Val Gln Leu Lys Asp Gln Ile Leu Gly Val 1355 1360 1365 Leu Asp Tyr Leu Glu Lys Gln Gln Ser Ala Trp Pro Phe Leu Lys 1370 1375 1380 Pro Val Ser Leu Ser Glu Ala Pro Asp Tyr Tyr Asp Ile Ile Lys 1385 1390 1395 Glu Pro Thr Asp Ile Leu Thr Met Arg Arg Lys Ala Arg His Gly 1400 1405 1410 Asp Tyr Lys Thr Lys Glu Asp Phe Gly Ile Glu Leu Lys Arg Met 1415 1420 1425 Phe Asp Asn Cys Arg Leu Tyr Asn Ala Pro Thr Thr Ile Tyr Phe 1430 1435 1440 Lys Tyr Ala Asn Glu Leu Gln Thr Leu Ile Trp Pro Lys Tyr Glu 1445 1450 1455 Ala Ile Thr Asp Thr Ala Lys 1460 1465 212034DNAPlasmodium falciparum 21atggctagtg caaaaggttc aaaaccaaat ttaccagaat ccaatatcgc tattggaatt 60gatttaggta ctacttattc ttgtgttggt gtatggagaa atgaaaatgt agatattatt 120gctaatgacc aaggtaatag aacaacccca tcttatgttg ctttcaccga taccgaaaga 180ttaattggag atgctgctaa aaaccaagta gctaggaatc cagaaaatac agtatttgat 240gctaagagat taattggtag aaaatttaca gaatcatcag tacaaagtga tatgaaacat 300tggccattca ctgttaaatc aggtgttgat gagaaaccaa tgattgaagt tacctatcaa 360ggagaaaaga aattattcca tccagaagaa atttcttcta tggtattaca aaaaatgaaa 420gaaaatgctg aagcattttt aggaaaatct ataaagaatg ctgtcattac cgttccagct 480tattttaacg attcacaaag acaagctact aaagatgctg gtacaattgc aggattaaat 540gttatgagaa ttattaatga acctactgca gctgctattg catatggttt acacaaaaaa 600ggaaaaggtg aaaagaacat tttaattttc gacttaggag gaggtacatt tgatgtatca 660ttattaacta ttgaagatgg tatttttgaa gtaaaagcta ctgctggtga tactcattta 720ggtggtgaag atttcgataa cagattagta aatttctgtg ttgaagattt caaaagaaaa 780aacagaggta aagatttatc aaaaaatagt agagccttaa gaagattaag aacacaatgt 840gaaagagcaa aacgtacttt atcatcatct acacaagcta caattgaaat agattcctta 900tttgaaggta ttgattacag tgttactgta agtagagcaa gatttgaaga attatgtatc 960gactatttcc gtgatacttt aattccagta gaaaaagttt taaaagatgc tatgatggat 1020aaaaaaagtg tacatgaagt tgttttagtt ggtggttcta caagaattcc aaaaatccaa 1080actttaataa aagaattctt taatggtaaa gaagcatgca gatcaattaa cccagatgaa 1140gctgttgcat atggtgcagc tgtacaagca gccattttat ctggtgacca atcaaatgct 1200gtccaagatt tattattatt agatgtttgc tccttatcat taggtttaga aactgctggt 1260ggtgttatga ccaaattaat tgaaagaaac acaaccatac ctgctaaaaa gagtcaaatc 1320tttactactt atgctgataa ccaaccaggt gtcttaattc aagtatatga aggtgaaaga 1380gccttaacca aagataacaa tttattagga aaatttcact tagatggtat tccacctgca 1440ccaagaaagg taccacaaat cgaagttaca ttcgatatcg atgctaacgg tatcttaaac 1500gttacggctg tagaaaaatc cactggtaaa caaaaccata ttacaattac caacgacaaa 1560ggaagattat ctcaagatga aattgatcgt atggttaatg atgctgaaaa atacaaagca 1620gaagatgaag aaaacagaaa aagaatcgaa gcaagaaaca gccttgaaaa ttactgctat 1680ggagttaaaa gctcattaga agaccaaaaa attaaagaaa aattacaacc agctgaaatt 1740gaaacatgta tgaaaactat tacaaccata cttgaatggt tagaaaaaaa ccaacttgct 1800ggaaaagatg aatatgaagc caaacaaaaa gaagcagaat cggtttgtgc tccaattatg 1860tctaaaatct atcaagatgc tgctggtgca gccggtggta tgccaggagg tatgcccggt 1920ggaatgcccg gtggaatgcc aggtggtatg aatttcccag gaggtatgcc cggagcagga 1980atgccaggaa atgccccagc tggaagtgga ccaacagttg aagaagttga ttaa 203422677PRTPlasmodium falciparum 22Met Ala Ser Ala Lys Gly Ser Lys Pro Asn Leu Pro Glu Ser Asn Ile 1 5 10 15 Ala Ile Gly Ile Asp Leu Gly Thr Thr Tyr Ser Cys Val Gly Val Trp 20 25 30 Arg Asn Glu Asn Val Asp Ile Ile Ala Asn Asp Gln Gly Asn Arg Thr 35 40 45 Thr Pro Ser Tyr Val Ala Phe Thr Asp Thr Glu Arg Leu Ile Gly Asp 50 55 60 Ala Ala Lys Asn Gln Val Ala Arg Asn Pro Glu Asn Thr Val Phe Asp 65 70 75 80 Ala Lys Arg Leu Ile Gly Arg Lys Phe Thr Glu Ser Ser Val Gln Ser 85 90 95 Asp Met Lys His Trp Pro Phe Thr Val Lys Ser Gly Val Asp Glu Lys 100 105 110 Pro Met Ile Glu Val Thr Tyr Gln Gly Glu Lys Lys Leu Phe His Pro 115 120 125 Glu Glu Ile Ser Ser Met Val Leu Gln Lys Met Lys Glu Asn Ala Glu 130 135 140 Ala Phe Leu Gly Lys Ser Ile Lys Asn Ala Val Ile Thr Val Pro Ala 145 150 155 160 Tyr Phe Asn Asp Ser Gln Arg Gln Ala Thr Lys Asp Ala Gly Thr Ile 165 170 175 Ala Gly Leu Asn Val Met Arg Ile Ile Asn Glu Pro Thr Ala Ala Ala 180 185 190 Ile Ala Tyr Gly Leu His Lys Lys Gly Lys Gly Glu Lys Asn Ile Leu 195 200 205 Ile Phe Asp Leu Gly Gly Gly Thr Phe Asp Val Ser Leu Leu Thr Ile 210 215 220 Glu Asp Gly Ile Phe Glu Val Lys Ala Thr Ala Gly Asp Thr His Leu 225 230 235 240 Gly Gly Glu Asp Phe Asp Asn Arg Leu Val Asn Phe Cys Val Glu Asp 245 250 255 Phe Lys Arg Lys Asn Arg Gly Lys Asp Leu Ser Lys Asn Ser Arg Ala 260 265 270 Leu Arg Arg Leu Arg Thr Gln Cys Glu Arg Ala Lys Arg Thr Leu Ser 275 280 285 Ser Ser Thr Gln Ala Thr Ile Glu Ile Asp Ser Leu Phe Glu Gly Ile 290 295 300 Asp Tyr Ser Val Thr Val Ser Arg Ala Arg Phe Glu Glu Leu Cys Ile 305 310 315 320 Asp Tyr Phe Arg Asp Thr Leu Ile Pro Val Glu Lys Val Leu Lys Asp 325 330 335 Ala Met Met Asp Lys Lys Ser Val His Glu Val Val Leu Val Gly Gly 340 345 350 Ser Thr Arg Ile Pro Lys Ile Gln Thr Leu Ile Lys Glu Phe Phe Asn 355 360 365 Gly Lys Glu Ala Cys Arg Ser Ile Asn Pro Asp Glu Ala Val Ala Tyr 370 375 380 Gly Ala Ala Val Gln Ala Ala Ile Leu Ser Gly Asp Gln Ser Asn Ala 385 390 395 400 Val Gln Asp Leu Leu Leu Leu Asp Val Cys Ser Leu Ser Leu Gly Leu 405 410 415 Glu Thr Ala Gly Gly Val Met Thr Lys Leu Ile Glu Arg Asn Thr Thr 420 425 430 Ile Pro Ala Lys Lys Ser Gln Ile Phe Thr Thr Tyr Ala Asp Asn Gln 435 440 445 Pro Gly Val Leu Ile Gln Val Tyr Glu Gly Glu Arg Ala Leu Thr Lys 450 455 460 Asp Asn Asn Leu Leu Gly Lys Phe His Leu Asp Gly Ile Pro Pro Ala 465 470 475 480 Pro Arg Lys Val Pro Gln Ile Glu Val Thr Phe Asp Ile Asp Ala Asn 485 490 495 Gly Ile Leu Asn Val Thr Ala Val Glu Lys Ser Thr Gly Lys Gln Asn 500 505 510 His Ile Thr Ile Thr Asn Asp Lys Gly Arg Leu Ser Gln Asp Glu Ile 515 520 525 Asp Arg Met Val Asn Asp Ala Glu Lys Tyr Lys Ala Glu Asp Glu Glu 530 535 540 Asn Arg Lys Arg Ile Glu Ala Arg Asn Ser Leu Glu Asn Tyr Cys Tyr 545 550 555 560 Gly Val Lys Ser Ser Leu Glu Asp Gln Lys Ile Lys Glu Lys Leu Gln 565 570 575 Pro Ala Glu Ile Glu Thr Cys Met Lys Thr Ile Thr Thr Ile Leu Glu 580 585 590 Trp Leu Glu Lys Asn Gln Leu Ala Gly Lys Asp Glu Tyr Glu Ala Lys 595 600 605 Gln Lys Glu Ala Glu Ser Val Cys Ala Pro Ile Met Ser Lys Ile Tyr 610 615 620 Gln Asp Ala Ala Gly Ala Ala Gly Gly Met Pro Gly Gly Met Pro Gly 625 630 635 640 Gly Met Pro Gly Gly Met Pro Gly Gly Met Asn Phe Pro Gly Gly Met 645 650 655 Pro Gly Ala Gly Met Pro Gly Asn Ala Pro Ala Gly Ser Gly Pro Thr 660 665 670 Val Glu Glu Val Asp 675 23999DNAPlasmodium falciparum 23atgaatgcag ccgctgtaga acttattaat agattaaaga aagaagatga aagcaataac 60aaatgttttg attgtggaat atccaacccc gattgggtgt ctgtaaatca tggtatattt 120ttatgtatta actgttcagg tgttcacaga agcctgggtg tacatataag tatagtaaga 180agtataaaaa tggatatatt tacagatgaa caattaaaat atatagataa gggaggtaat 240aaaaaatgtc aaacatattt agagaattat ggaataagtg attttattcc agaaagaaaa 300tatagaacta aggcagctga tcattatagg aaaatattaa gatcgattgt tcataatgtg 360gatcctcctg ctcctttacc tttagatgaa ggaaagagtt taattaatta tggaagaaat 420gaaaatgtta atgaaaataa taaaaatcaa tattcaaatg aagaaccgga ttttatttca 480tcattaaata catcagaaat tatagaaaat gtaagtgcca cattttccaa tttaataaat 540aaagcatcaa gtatgacaac taatacaata aataatttaa atactaatga tttaatagaa 600tcaactaaag gtacaataat caatagtgga agctggttta ctgagaaaac taaaaaaatt 660gcagaaaatg ttagtgataa tccatggtgg gctaaaggac aaagtaaaat aaaagatgtt 720acacaaaatg ctagtggatg gatatctacc atttcttcaa cagtttcaag aacaaatagt 780aatttctttt tttcaaataa tgatactaat ataaataatg gaaacaatga agatatcaat 840aataataata ctaacgttaa tagtaatggt aatactacta ataataataa tgaacaagca 900caaaataata ataatgttaa ttttaattat aatagttcaa atgcaaataa tgaatattta 960aattacggaa tgaatgaaaa taattcaact tttaattga 99924332PRTPlasmodium falciparum 24Met Asn Ala Ala Ala Val Glu Leu Ile Asn Arg Leu Lys Lys Glu Asp 1 5 10 15 Glu Ser Asn Asn Lys Cys Phe Asp Cys Gly Ile Ser Asn Pro Asp Trp 20 25 30 Val Ser Val Asn His Gly Ile Phe Leu Cys Ile Asn Cys Ser Gly Val 35 40 45 His Arg Ser Leu Gly Val His Ile Ser Ile Val Arg Ser Ile Lys Met 50 55 60 Asp Ile Phe Thr Asp Glu Gln Leu Lys Tyr Ile Asp Lys Gly Gly Asn 65 70 75 80 Lys Lys Cys Gln Thr Tyr Leu Glu Asn Tyr Gly Ile Ser Asp Phe Ile 85 90 95 Pro Glu Arg Lys Tyr Arg Thr Lys Ala Ala Asp His Tyr Arg Lys Ile 100 105 110 Leu Arg Ser Ile Val His Asn Val Asp Pro Pro Ala Pro Leu Pro Leu 115 120 125 Asp Glu Gly Lys Ser Leu Ile Asn Tyr Gly Arg Asn Glu Asn Val Asn 130 135 140 Glu Asn Asn Lys Asn Gln Tyr Ser Asn Glu Glu Pro Asp Phe Ile Ser 145 150 155 160 Ser Leu Asn Thr Ser Glu Ile Ile Glu Asn Val Ser Ala Thr Phe Ser 165 170 175 Asn Leu Ile Asn Lys Ala Ser Ser Met Thr Thr Asn Thr Ile Asn Asn 180 185 190 Leu Asn Thr Asn Asp Leu Ile Glu Ser Thr Lys Gly Thr Ile Ile Asn 195 200 205 Ser Gly Ser Trp Phe Thr Glu Lys Thr Lys Lys Ile Ala Glu Asn Val 210 215 220 Ser Asp Asn Pro Trp Trp Ala Lys Gly Gln Ser Lys Ile Lys Asp Val 225 230 235 240 Thr Gln Asn Ala Ser Gly Trp Ile Ser Thr Ile Ser Ser Thr Val Ser 245 250 255 Arg Thr Asn Ser Asn Phe Phe Phe Ser Asn Asn Asp Thr Asn Ile Asn 260 265 270 Asn Gly Asn Asn Glu Asp Ile Asn Asn Asn Asn Thr Asn Val Asn Ser 275 280 285 Asn Gly Asn Thr Thr Asn Asn Asn Asn Glu Gln Ala Gln Asn Asn Asn 290 295 300 Asn Val Asn Phe Asn Tyr Asn Ser Ser Asn Ala Asn Asn Glu Tyr Leu 305 310 315 320 Asn Tyr Gly Met Asn Glu Asn Asn Ser Thr Phe Asn 325 330 251194DNAPlasmodium falciparum 25atgatgagaa aattagctat tttatctgtt tcttcctttt tatttgttga ggccttattc 60caggaatacc agtgctatgg aagttcgtca aacacaaggg ttctaaatga attaaattat 120gataatgcag gcactaattt atataatgaa ttagaaatga attattatgg gaaacaggaa 180aattggtata gtcttaaaaa aaatagtaga tcacttggag aaaatgatga tggaaataac 240gaagacaacg agaaattaag gaaaccaaaa cataaaaaat taaagcaacc agcggatggt 300aatcctgatc caaatgcaaa cccaaatgta gatcccaatg ccaacccaaa tgtagatcca 360aatgcaaacc caaatgtaga tccaaatgca aacccaaatg caaacccaaa tgcaaaccca 420aatgcaaacc caaatgcaaa cccaaatgca aacccaaatg caaacccaaa tgcaaaccca 480aatgcaaacc caaatgcaaa cccaaatgca aacccaaatg caaacccaaa tgcaaaccca 540aatgcaaacc ccaatgcaaa tcctaatgca aacccaaatg caaacccaaa cgtagatcct 600aatgcaaatc caaatgcaaa cccaaacgca aaccccaatg caaatcctaa tgcaaacccc 660aatgcaaatc ctaatgcaaa tcctaatgcc aatccaaatg caaatccaaa tgcaaaccca 720aacgcaaacc ccaatgcaaa tcctaatgcc aatccaaatg caaatccaaa tgcaaaccca 780aatgcaaacc caaatgcaaa ccccaatgca aatcctaata aaaacaatca aggtaatgga 840caaggtcaca atatgccaaa tgacccaaac cgaaatgtag atgaaaatgc taatgccaac 900agtgctgtaa aaaataataa taacgaagaa ccaagtgata agcacataaa agaatattta 960aacaaaatac aaaattctct ttcaactgaa tggtccccat gtagtgtaac ttgtggaaat 1020ggtattcaag ttagaataaa gcctggctct gctaataaac ctaaagacga attagattat 1080gcaaatgata ttgaaaaaaa aatttgtaaa atggaaaaat gttccagtgt gtttaatgtc 1140gtaaatagtt caataggatt aataatggta ttatccttct tgttccttaa ttag 119426397PRTPlasmodium falciparum 26Met Met Arg Lys Leu Ala Ile Leu Ser Val Ser Ser Phe Leu Phe Val 1 5 10 15 Glu Ala Leu Phe Gln Glu Tyr Gln Cys Tyr Gly Ser Ser Ser Asn Thr 20 25 30 Arg Val Leu Asn Glu Leu Asn Tyr Asp Asn Ala Gly Thr Asn Leu Tyr 35 40 45 Asn Glu Leu Glu Met Asn Tyr Tyr Gly Lys Gln Glu Asn Trp Tyr Ser 50 55 60 Leu Lys Lys Asn Ser Arg Ser Leu Gly Glu Asn Asp Asp Gly Asn Asn 65 70

75 80 Glu Asp Asn Glu Lys Leu Arg Lys Pro Lys His Lys Lys Leu Lys Gln 85 90 95 Pro Ala Asp Gly Asn Pro Asp Pro Asn Ala Asn Pro Asn Val Asp Pro 100 105 110 Asn Ala Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Val Asp Pro 115 120 125 Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro 130 135 140 Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro 145 150 155 160 Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro 165 170 175 Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro 180 185 190 Asn Ala Asn Pro Asn Val Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro 195 200 205 Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro 210 215 220 Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro 225 230 235 240 Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro 245 250 255 Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro 260 265 270 Asn Lys Asn Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro Asn Asp 275 280 285 Pro Asn Arg Asn Val Asp Glu Asn Ala Asn Ala Asn Ser Ala Val Lys 290 295 300 Asn Asn Asn Asn Glu Glu Pro Ser Asp Lys His Ile Lys Glu Tyr Leu 305 310 315 320 Asn Lys Ile Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val 325 330 335 Thr Cys Gly Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asn 340 345 350 Lys Pro Lys Asp Glu Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys Ile 355 360 365 Cys Lys Met Glu Lys Cys Ser Ser Val Phe Asn Val Val Asn Ser Ser 370 375 380 Ile Gly Leu Ile Met Val Leu Ser Phe Leu Phe Leu Asn 385 390 395 272526DNAPlasmodium falciparum 27atgtcggctt ttgaagagtt ggggttacat agcgatttgt gcgcagtttt agaaaaaaat 60ggcattgatt taccaacggc tattcaacaa gagtccattc ctctgacatt agggggagga 120gatttatgtg cttgtgctga aactggtaca ggaaagacgt taagtttcat attttcttct 180ttacaaatag ttcatgaatt ggttcgaaat ataggtaact atgaagatgt gagtattaga 240aaaaataaca ataacatgga aaataataat gatagtaata tttctaacat tatagatgat 300aatgataaga ataagaagtc aagttacata aaaactatta ataagaactc aaaagtaata 360attaaagaca atgaatgtat atgtaataat aataataata ataatagtaa taatttcctt 420ttcgaagaag taaaagttga ctgtgaaata acgaatggat tatatgcata cgaaattgaa 480atattatcta aaagttatgt gaatgttggt ttttgtccat ccattaaaga aacattaaaa 540tataattata cctactgtag taatggtaat aaatataata ataatagaca agaacattat 600ggtgaatatt tttcaaataa tgatattata acatgtttaa ttaataaaaa taataatact 660atatctttta aaaaaaatgg gaaattttta ggtaacgctt ttaaaatttt ttataaatat 720aatgatgttg cattttttcc atatatatgg ggaaaatatt tccatataaa acttcatttc 780gaaaatttaa aatatggtga tcgttcatta cattttaatg aactatgtat tttattaact 840tcaaattgta atgataatga tactttaagt gataggaaaa tatttttttc gtctgaaaat 900ataagggacg aaaaaaattt taaattagag aaagcaaata tgcaaacaca taattatcaa 960aataataata gtataataaa cgaatataca cataattata atcaacaaaa tttcaataat 1020aaaaaaaaac tatactgtat tgttttatgt cctactagag atttagcaca acaaacttat 1080aataattatt taacgtactc tgaatgtttt aataaccctt ctattaatat tggaatatta 1140gtaggaggag aacaaagaaa taaaggtcat catatgaatg acgacggatc ttataatatt 1200gttgtgggta catgctttaa attaattgaa tgtataagaa aaaattctat aaaagttaat 1260gatataagat tattaatatt agatgaagca gatgaattaa taaataatga tgaaaaaaca 1320gttttagaaa ttaaagattc ttgtatgaaa tatggacatc gtgtgcaaac tttatttttt 1380tctgctactt tgcaagacaa aaatgttaaa gattgtatta ataaaataac caataaacca 1440atatttgttg atttgaaata tggaaaaaat agtataccat cacacatata tgtgtgtcta 1500tattatgtca atgatgaaaa ttcgaattta tcgtatatta taaaagaaaa tgataataat 1560aatcataaca aagaaatata taatatcctt tataatgaga aattatatca tatgaataca 1620agggaattat atgaatatac agataaagta catatcttaa ataattcaaa tagtaattta 1680aaaaaaaatg ataaggaaca aatttcttta aatataaaaa tgaataaact aaaaaaatta 1740gttcaaataa ttaatgtttt taatatgcaa aatggtatta tattttgtcg aaccaattta 1800gattgtgaca atgtatataa ttttttaaat gctgtaggtg atggtaaagc ttataaaggt 1860accgttgaat cacttaaaga aaataaatat tcctgtgtta tattaaaagg aaaaatgtca 1920aatgacgaaa gaaaaaacaa tcttcaagct ttcaaaaaag gtgaagtacg ttttttaata 1980tgtacagatg tcgctgcaag aggtattgat atacagaatt taagatatct tattattatg 2040accttgtctg ataatattaa tacatttttt cataaaattg gtagagtagg aagagatgga 2100aaaaacagtt tatgtattgt cttaagtgca gataatgagc aagaagaaaa ggtatggttt 2160catacatgtc ctagtagagg aattaattgt tacaatagaa atttgaaaga aaataaagga 2220tgtactgtat atattaaaga aagtgattat attaaaacta ttaatgatat gttagaagta 2280cctatacatg tgttagattc caaatattat tatgctgaaa atgttgttga tcctttaaat 2340tattttaaaa aacatccagt ttctaataaa agcagaagaa ataaaaatca aaatgctaac 2400tccatttttc aagaatcatc ccatatagat gtgttatctt cctttgcttc aaacattaat 2460agtataaaaa aattacagtc tgctattagt tataagcatt atgaattgtt gaactttcaa 2520atatga 252628841PRTPlasmodium falciparum 28Met Ser Ala Phe Glu Glu Leu Gly Leu His Ser Asp Leu Cys Ala Val 1 5 10 15 Leu Glu Lys Asn Gly Ile Asp Leu Pro Thr Ala Ile Gln Gln Glu Ser 20 25 30 Ile Pro Leu Thr Leu Gly Gly Gly Asp Leu Cys Ala Cys Ala Glu Thr 35 40 45 Gly Thr Gly Lys Thr Leu Ser Phe Ile Phe Ser Ser Leu Gln Ile Val 50 55 60 His Glu Leu Val Arg Asn Ile Gly Asn Tyr Glu Asp Val Ser Ile Arg 65 70 75 80 Lys Asn Asn Asn Asn Met Glu Asn Asn Asn Asp Ser Asn Ile Ser Asn 85 90 95 Ile Ile Asp Asp Asn Asp Lys Asn Lys Lys Ser Ser Tyr Ile Lys Thr 100 105 110 Ile Asn Lys Asn Ser Lys Val Ile Ile Lys Asp Asn Glu Cys Ile Cys 115 120 125 Asn Asn Asn Asn Asn Asn Asn Ser Asn Asn Phe Leu Phe Glu Glu Val 130 135 140 Lys Val Asp Cys Glu Ile Thr Asn Gly Leu Tyr Ala Tyr Glu Ile Glu 145 150 155 160 Ile Leu Ser Lys Ser Tyr Val Asn Val Gly Phe Cys Pro Ser Ile Lys 165 170 175 Glu Thr Leu Lys Tyr Asn Tyr Thr Tyr Cys Ser Asn Gly Asn Lys Tyr 180 185 190 Asn Asn Asn Arg Gln Glu His Tyr Gly Glu Tyr Phe Ser Asn Asn Asp 195 200 205 Ile Ile Thr Cys Leu Ile Asn Lys Asn Asn Asn Thr Ile Ser Phe Lys 210 215 220 Lys Asn Gly Lys Phe Leu Gly Asn Ala Phe Lys Ile Phe Tyr Lys Tyr 225 230 235 240 Asn Asp Val Ala Phe Phe Pro Tyr Ile Trp Gly Lys Tyr Phe His Ile 245 250 255 Lys Leu His Phe Glu Asn Leu Lys Tyr Gly Asp Arg Ser Leu His Phe 260 265 270 Asn Glu Leu Cys Ile Leu Leu Thr Ser Asn Cys Asn Asp Asn Asp Thr 275 280 285 Leu Ser Asp Arg Lys Ile Phe Phe Ser Ser Glu Asn Ile Arg Asp Glu 290 295 300 Lys Asn Phe Lys Leu Glu Lys Ala Asn Met Gln Thr His Asn Tyr Gln 305 310 315 320 Asn Asn Asn Ser Ile Ile Asn Glu Tyr Thr His Asn Tyr Asn Gln Gln 325 330 335 Asn Phe Asn Asn Lys Lys Lys Leu Tyr Cys Ile Val Leu Cys Pro Thr 340 345 350 Arg Asp Leu Ala Gln Gln Thr Tyr Asn Asn Tyr Leu Thr Tyr Ser Glu 355 360 365 Cys Phe Asn Asn Pro Ser Ile Asn Ile Gly Ile Leu Val Gly Gly Glu 370 375 380 Gln Arg Asn Lys Gly His His Met Asn Asp Asp Gly Ser Tyr Asn Ile 385 390 395 400 Val Val Gly Thr Cys Phe Lys Leu Ile Glu Cys Ile Arg Lys Asn Ser 405 410 415 Ile Lys Val Asn Asp Ile Arg Leu Leu Ile Leu Asp Glu Ala Asp Glu 420 425 430 Leu Ile Asn Asn Asp Glu Lys Thr Val Leu Glu Ile Lys Asp Ser Cys 435 440 445 Met Lys Tyr Gly His Arg Val Gln Thr Leu Phe Phe Ser Ala Thr Leu 450 455 460 Gln Asp Lys Asn Val Lys Asp Cys Ile Asn Lys Ile Thr Asn Lys Pro 465 470 475 480 Ile Phe Val Asp Leu Lys Tyr Gly Lys Asn Ser Ile Pro Ser His Ile 485 490 495 Tyr Val Cys Leu Tyr Tyr Val Asn Asp Glu Asn Ser Asn Leu Ser Tyr 500 505 510 Ile Ile Lys Glu Asn Asp Asn Asn Asn His Asn Lys Glu Ile Tyr Asn 515 520 525 Ile Leu Tyr Asn Glu Lys Leu Tyr His Met Asn Thr Arg Glu Leu Tyr 530 535 540 Glu Tyr Thr Asp Lys Val His Ile Leu Asn Asn Ser Asn Ser Asn Leu 545 550 555 560 Lys Lys Asn Asp Lys Glu Gln Ile Ser Leu Asn Ile Lys Met Asn Lys 565 570 575 Leu Lys Lys Leu Val Gln Ile Ile Asn Val Phe Asn Met Gln Asn Gly 580 585 590 Ile Ile Phe Cys Arg Thr Asn Leu Asp Cys Asp Asn Val Tyr Asn Phe 595 600 605 Leu Asn Ala Val Gly Asp Gly Lys Ala Tyr Lys Gly Thr Val Glu Ser 610 615 620 Leu Lys Glu Asn Lys Tyr Ser Cys Val Ile Leu Lys Gly Lys Met Ser 625 630 635 640 Asn Asp Glu Arg Lys Asn Asn Leu Gln Ala Phe Lys Lys Gly Glu Val 645 650 655 Arg Phe Leu Ile Cys Thr Asp Val Ala Ala Arg Gly Ile Asp Ile Gln 660 665 670 Asn Leu Arg Tyr Leu Ile Ile Met Thr Leu Ser Asp Asn Ile Asn Thr 675 680 685 Phe Phe His Lys Ile Gly Arg Val Gly Arg Asp Gly Lys Asn Ser Leu 690 695 700 Cys Ile Val Leu Ser Ala Asp Asn Glu Gln Glu Glu Lys Val Trp Phe 705 710 715 720 His Thr Cys Pro Ser Arg Gly Ile Asn Cys Tyr Asn Arg Asn Leu Lys 725 730 735 Glu Asn Lys Gly Cys Thr Val Tyr Ile Lys Glu Ser Asp Tyr Ile Lys 740 745 750 Thr Ile Asn Asp Met Leu Glu Val Pro Ile His Val Leu Asp Ser Lys 755 760 765 Tyr Tyr Tyr Ala Glu Asn Val Val Asp Pro Leu Asn Tyr Phe Lys Lys 770 775 780 His Pro Val Ser Asn Lys Ser Arg Arg Asn Lys Asn Gln Asn Ala Asn 785 790 795 800 Ser Ile Phe Gln Glu Ser Ser His Ile Asp Val Leu Ser Ser Phe Ala 805 810 815 Ser Asn Ile Asn Ser Ile Lys Lys Leu Gln Ser Ala Ile Ser Tyr Lys 820 825 830 His Tyr Glu Leu Leu Asn Phe Gln Ile 835 840 297962DNAPlasmodium falciparum 29atgatgagtg atgcaaacaa aagacagcta agaacgagaa gaaaaaaaac tatgaattca 60ttatattata atagtgatta tagtaatgat ataaatatag aagattctaa aagtagatta 120agtggtggca agaaaagtga gggtagttct agtaaatcaa aagatgttat atcatcttat 180actaagaaat atagccttaa taataaaaat cataaacata gtaaaaatat tatgaatcat 240aaaaatatga tttatagtga tgggtatatg aataatatga atgaagacaa tgtagacaat 300gaagaaattt gtgagaatca tttgtatgag gaaaataatg ataataatat gaatgatgat 360atgaatgaaa ataatataat agatgaagat gaaaatgaaa atacaaatct tgtaaatata 420gacgatgaaa atattaatga tgataataat aatgaaaata atgataatat tgaagacatg 480actaatgaca tgaataataa tgtaagtcat aataatatta ttagcaatga taattcatat 540aataatcaaa tagatagtac atttcttaat atgaataata aaagaaaaga tgaggaacat 600ataaataatg atgatgaata tatacataaa agtaaagata agaaaaaaag tggatcatta 660gtacatataa aaaatgaaaa tacaaataaa ttagataata gtaataataa taataataat 720aataaaaata aaaaagatag tgtaagtgca aatggatcta ataagaataa ttgtaataca 780tctagtagta ataatcgtag tagacatgta cctaataata acgaaataaa ttatgcaaaa 840atggctgaaa aattacctca tgttgttggg gttagatttg ataagtcaca aaatagatgg 900ctatctggta tatgtattaa tggacgatgt attaacagat atttcccagt atataaattt 960ggatttgaag aagctcgtcg gttagccatt caacatagga aaaattttga gacagctaat 1020gtaggtcatt taaaaaaaca acagggtgag gctaaaacgt ctcacttgaa tttgttgaac 1080cttaactcgc aaatacccaa tgactttaaa gatatccaag gaaaaaataa acttattttc 1140aaataccttt cttatgactc cattcaaaaa gagtgggttg taacatacga ttatgacaac 1200aaagttctcg ttaataaatt tcctgtagat atatatggat acaataatgc ctacgaaatg 1260gctgtccatt gtatcaataa gcttaatata tgtaatcagg aaaagttgtg taaaaatatt 1320aataaacaag agcattcaag aggattacaa ggtgatttgt taaataattt taatgataat 1380aatattaata ataataataa tatgaatgat aacaataata ataatatact tgataataat 1440aataatttga ttgataataa taataataat aatatttcga ttgataatca caatttgaat 1500atatataata attccagtga acataataat atgtgtgaag atttgagttc ttttaaaata 1560ccccaaaaca aaataactaa gttaattaat tcagggcaat caaatttttt aatgaataat 1620caaaaatcag aaatggcttt gaacgatatt cttaataatg agaatatgat gaataattta 1680attcatgaaa agcaaaaact gaatataaac cataataata ttaataaaaa attagaagaa 1740ttagaaaata tgagaaaaga agatacaatg tttttaaatg acagttcttc ttatttaagt 1800accaacgata taaaagtatt gaatggtatt aaaagattaa aaacagataa ttctcctttt 1860aataaacagg atgaggaatt tttattaaat tctaaaaatt tgaataaata ttctactatg 1920caaaacaata aatcttatga tattttaaat gattcaaatt ttaatatatt agaaaatgaa 1980ggaatgtttt ctttaaatga taaaaaaggt ataaataata acgatatgtc aaaaaataga 2040aatattacaa attctaatta ttttaaacca ttccatgatg aagcagaaaa taatatatat 2100cagttattaa atggaaaata tttaaccaat gaggataaaa gtgtacatat caatcttttg 2160aatagtgtaa tgaagcaaag tactatgaac aaattatcaa atgaagaaga tctttttgaa 2220aaaaaagaaa aaaaaagaaa attaaagaat aatatgcttg aacaaaaagg agatgatcaa 2280tttataaata tgaacaaaag gatcgatacg aacttagtga gtaataataa taatataaat 2340gcatttaaca acaacaataa taataatatt gtcaataata atattgtcaa taataatatt 2400gtcagtaata atattgtcag taataatatt gtcagtaata atattagcaa taataatatt 2460atcaataata ataataatgt aatccctttt ggtgatgaac attttgatgc cttgtcattc 2520gatgagtatc gaaaacgtat taatagtaaa aagagtaatt attcaaatat aggagaaaat 2580gaaaaataca agaaattagc taacgggttg ttagataatg gtgaaacact atctaaaaat 2640ttaaatgaat taaaaatgta tcatttggca ggaaaaaaag aaaaagataa taatatgaac 2700aatatgaata ataacatttt atataataat aataatatga aatattatac acataacgaa 2760catcataaga atgaagaatt aaaaaggagt caacttaatg aaaatgtgaa tcatgagaat 2820atgctaaatt tgaatcaaat gataagaaat atacttaata ataatagtgt agataataat 2880gaaaaaataa taaaaggtat aaatatctta aaaaatatga ctatgaatga taaatttaat 2940ttgattaatt ttaataatca atgcatgaat tattcgtata acaatatgat gaaaaaggaa 3000aattatgtta aaaatatata taaaattatt aataagggtt cagaggattc tattaattat 3060gatgaatatt taactgtcaa gttagaagtt gaagatggtg aagaaaaaga tcaaaatggt 3120gaaaaaaaaa aaatcaacac atcagaggca tatcatgaag gaaaaatgaa taaaaaacat 3180aaaaagaaaa aaaaaatgaa gcaaaataaa ttagatgaag atattactgt aaataatgga 3240atgataaatg aaggagacaa tgacaaaatt gaagatacta ataaaaatga cgatatatca 3300aataattcag aagttacaaa taaagatatt ataaatggtg aaagaaattc aaatgattct 3360gaagaaaagg agaaggaaga aaaagaagat tataatgaaa atacatataa taatatgaat 3420acagataata atacatcaaa cagtaatgat gataatgaac aaataattaa taacgaaaat 3480gattatatat taaacaaaac aaataatgaa attaataaag aaataaatac atctataaat 3540gaatttaatg atattgcttt ccaattgtgt ccttggaaaa aaggtattca atggaatttg 3600ttaaaaaaaa tgtggacctg taaattatgg gataataaag gtaatgaaat tacaaaaaat 3660attcatataa aaaaaaaaga aggtattgat attggatata aatattgtat aaaaattcga 3720acaaattctt ttatatttta tctatcaaaa gaattaaata aatttccaca tattcctgaa 3780atatcttacg acttacaaaa tcttcatttt gttgtatctt ataaagataa tgaaaagaaa 3840atatattcct ttgaagatgg aatttataaa tcatttatag actgtatgga atatctaaac 3900aaacaaaaaa gagaatataa tgaaaatatt tatgatatat ccaattttat aaaagaaaaa 3960aataatttgg aattagatga tatatataaa gaattaaatt atttcgaata taatagaaat 4020gtattaagta atatttgtga agaaacaaaa attgcatata gtataaaacc atggttaaga 4080ggtattatat gggaagataa attgaaaaaa tggatagtct tttttaaaga taaaaatgaa 4140aacttgagaa ttagcttttt tagtacatca gattataacg atgatgttat tatgtcttat 4200aataaagctt gtgaatattt ttcacaagtt aaagaatcaa ataattttga ttccaattca 4260gaggaatttg ctcagtccat taaaaatgtt attaaaaatg ttcaatttga taaaataata 4320gaacaacaaa atgacgcaaa taataatgat cacaagaaag gtaatttgca agaggaccat 4380gatatacaaa caaattatta taataaggtg acacaaggaa atgaagggga aacaaataat 4440aataataata ataatcaagg agaaaatatg accgactctc aaattgtctt aaatacaaat 4500gaaatccatg tccataatgt acatacatta aaacgtcctg aaggattata taatattgca 4560gaaacacaat caacccattt tttaaaagat aataatattt cgaatgagca caaaagtatg 4620gaaacatttt ataatgaaaa ctcttattat aatgatgata ttgcttttct aaattatgct 4680gatagtaatg atagtcttat ttcaaaagga atgtctcaat atttatcatt accatcaaaa 4740aaaaagaaga aaaaaagaac tactgaagat atatataaaa gcgaaattga tgttttatct

4800tatcaaaata atgaattata tagcaatgta aaaaatgaaa actatgatga aaatatttca 4860gagaataata aaaaatatgg aaaaaggaaa atggaattga attttttaag tcacagtgaa 4920aataatataa ataatataaa taataatact tataataata atgattatac aaatgatgat 4980aatcatagta gtaaggtgta ttcttctgat tgtgaaaaga atactaatca taacaaaaag 5040acggaagata aatatgatca agagaacaat aaatattttt ataataaaga taaattatta 5100tttgaccacg aaaatggtac aattccaaga aatataaaga aggaatcaaa caatttaaac 5160agcgaatata atttggatta tttaaattct gaagatatta aaaataataa ccatcaccat 5220cacaataata ataataatat tattattaat ggaccatcaa gtgatggaga aacaataatt 5280ttatcacagt atcacaaaaa tgatgatgat gaagatgatg gaaataatac agataattca 5340aaaatattaa gaagttttga taagtgctca aatgaaaata gtgtatgtat aaataaaagt 5400aatgagaaac ttgaaaacat tttagacgga ggagtaaata tgagattacc aaaaagtgaa 5460attttaaatc aagcttctag attacccaaa ttacaaggta tgttttttga taagagaaga 5520aattattgga ctgttagtgt ttgtggtttc agaaaatcat ttggtgttag gacaagaggt 5580gtatatcaag catacaaatt agcagctgaa tttcgtaatc gtatattaga aaacaataaa 5640ggtaaatatt atcctcaaag aagtacaagt atgtctagta attataaata taatacaaag 5700ggtggatcca taattattaa agatgaaaaa ttgaattata gtaatatatc tgaaatgaca 5760tctgtagatg aagaatatga tccaaaaaaa aaaaaaagtt cgttttcttc taataatatt 5820aataatattg ataaggaatt atattcatta aatgacaaag aaagtaatag tagtggatta 5880tattattctt ttaatgtaaa agggaaaacc agttcaaata tatatgatta ttttttagat 5940aaaaaagaac atgactattt cgcaacaaat aatagtagta ataatataaa taataataat 6000aataataata ataatgatat aaataataat agtactaatg atataaataa taatggtact 6060aatgatacaa ataataagaa agatccaaaa ggtataccta ataattcatg ttatgatgat 6120caatataata atattgaaaa tgaagaggaa gaagaagaaa gggaagggga agaagctgaa 6180aggtggaata tatcagataa agtgaataat aaaaataatg ataataaaat tcaaattggt 6240aataatactt ttggacaatt aaaatatgat agtgctttag aatcaaacct tatcaatttt 6300ataaacgagg taggttcatc tgattttttg aaagatgcca aaattaataa taataataat 6360aacaataata ataataataa tattggtgga aataaaaaga atcaacatat aaataataat 6420attagcaata gtaatacttt aaataatagc ttaaatgttc aaaatatttc ttctagtaat 6480agtgctataa gtaataatgg agttataaat aattattcac ctacacatat atatgaagta 6540gacaatctca ataatagtca agattactat ttaaaaaatg acgaaatgaa aaatgaatta 6600aataaagtgg aagatcaaaa aaataaaaag gatatttcaa gcacaaatag tctactaaaa 6660cattttatga atgatgatat attaataaat aataaagtaa aacaacaacc cttttttgat 6720agtcacaaca attataataa gttaggacac aaacaagtca aaacatctgc ttatacggaa 6780agtgcaattg gattaggctt tagaaaaaat ggatatatta atgattgtga cttagttaat 6840aataataata ataataataa taataacatg catataaaga acaatggtgt tatattaaat 6900aaattgatat cttctaacca cgatagtaaa gataacagta ataacaatat taaggataaa 6960gatagggaag ataataataa aaatatttat aataataata atgatagatc tattggtagt 7020tatgtaaaca atcttggtgt aatagaaaac caatgtgaga atagtaaagg agcagatgaa 7080aacacgaaaa attttaataa taataaaaca ttaaataatt atgaaccaga aaataagtct 7140tataattaca gtccagtgtt agatgaaact ctagaagaaa gagatgtgag aataaataga 7200gaggtaagca aatgtaaatt tgtagatgga ttaatatacg atgaagctaa taaatgtttt 7260cgaataaaaa ttaatggtta tagaaaagca tattctgtta ttagaagagg agtaaaagaa 7320gcttataaat taagtattga atctatagaa caaattaaaa gacaaacaca aacaaataat 7380tttaataata atgaacagtc acaagttaat acaaataata tgacacagat atataattca 7440agttctgaaa attcaagaca tggatcatgt tataattatc aacataatca taaaagtaaa 7500ctagagaatt cgaatgataa tcctgaagaa aaatatgacc acatcaataa tatgaccaat 7560gtaggacatg aaaataaaca gagcgctgaa gaaagtttgt ttccaatgtt aaatgtagat 7620gataaatatt atgaacttct taaaacatcc attattatat gtctgaatga tatacttatg 7680aattgtatac ctcaagtatt tcacctatac aaaaatatca atacatcaaa tgacataaaa 7740ttagaagata tattatatac tgaaaggaaa agaaaagaac agtccttaaa atatcatatt 7800gaatatacac aaaattctgt aggtgtgtca tcccttatac cttacttgaa attatttagt 7860accgaaattt taaataacgt tttaccatca gcacaatcct tggaaataca aagattaata 7920attcattcgt tggatataca aacatataat acattatatt aa 7962302653PRTPlasmodium falciparum 30Met Met Ser Asp Ala Asn Lys Arg Gln Leu Arg Thr Arg Arg Lys Lys 1 5 10 15 Thr Met Asn Ser Leu Tyr Tyr Asn Ser Asp Tyr Ser Asn Asp Ile Asn 20 25 30 Ile Glu Asp Ser Lys Ser Arg Leu Ser Gly Gly Lys Lys Ser Glu Gly 35 40 45 Ser Ser Ser Lys Ser Lys Asp Val Ile Ser Ser Tyr Thr Lys Lys Tyr 50 55 60 Ser Leu Asn Asn Lys Asn His Lys His Ser Lys Asn Ile Met Asn His 65 70 75 80 Lys Asn Met Ile Tyr Ser Asp Gly Tyr Met Asn Asn Met Asn Glu Asp 85 90 95 Asn Val Asp Asn Glu Glu Ile Cys Glu Asn His Leu Tyr Glu Glu Asn 100 105 110 Asn Asp Asn Asn Met Asn Asp Asp Met Asn Glu Asn Asn Ile Ile Asp 115 120 125 Glu Asp Glu Asn Glu Asn Thr Asn Leu Val Asn Ile Asp Asp Glu Asn 130 135 140 Ile Asn Asp Asp Asn Asn Asn Glu Asn Asn Asp Asn Ile Glu Asp Met 145 150 155 160 Thr Asn Asp Met Asn Asn Asn Val Ser His Asn Asn Ile Ile Ser Asn 165 170 175 Asp Asn Ser Tyr Asn Asn Gln Ile Asp Ser Thr Phe Leu Asn Met Asn 180 185 190 Asn Lys Arg Lys Asp Glu Glu His Ile Asn Asn Asp Asp Glu Tyr Ile 195 200 205 His Lys Ser Lys Asp Lys Lys Lys Ser Gly Ser Leu Val His Ile Lys 210 215 220 Asn Glu Asn Thr Asn Lys Leu Asp Asn Ser Asn Asn Asn Asn Asn Asn 225 230 235 240 Asn Lys Asn Lys Lys Asp Ser Val Ser Ala Asn Gly Ser Asn Lys Asn 245 250 255 Asn Cys Asn Thr Ser Ser Ser Asn Asn Arg Ser Arg His Val Pro Asn 260 265 270 Asn Asn Glu Ile Asn Tyr Ala Lys Met Ala Glu Lys Leu Pro His Val 275 280 285 Val Gly Val Arg Phe Asp Lys Ser Gln Asn Arg Trp Leu Ser Gly Ile 290 295 300 Cys Ile Asn Gly Arg Cys Ile Asn Arg Tyr Phe Pro Val Tyr Lys Phe 305 310 315 320 Gly Phe Glu Glu Ala Arg Arg Leu Ala Ile Gln His Arg Lys Asn Phe 325 330 335 Glu Thr Ala Asn Val Gly His Leu Lys Lys Gln Gln Gly Glu Ala Lys 340 345 350 Thr Ser His Leu Asn Leu Leu Asn Leu Asn Ser Gln Ile Pro Asn Asp 355 360 365 Phe Lys Asp Ile Gln Gly Lys Asn Lys Leu Ile Phe Lys Tyr Leu Ser 370 375 380 Tyr Asp Ser Ile Gln Lys Glu Trp Val Val Thr Tyr Asp Tyr Asp Asn 385 390 395 400 Lys Val Leu Val Asn Lys Phe Pro Val Asp Ile Tyr Gly Tyr Asn Asn 405 410 415 Ala Tyr Glu Met Ala Val His Cys Ile Asn Lys Leu Asn Ile Cys Asn 420 425 430 Gln Glu Lys Leu Cys Lys Asn Ile Asn Lys Gln Glu His Ser Arg Gly 435 440 445 Leu Gln Gly Asp Leu Leu Asn Asn Phe Asn Asp Asn Asn Ile Asn Asn 450 455 460 Asn Asn Asn Met Asn Asp Asn Asn Asn Asn Asn Ile Leu Asp Asn Asn 465 470 475 480 Asn Asn Leu Ile Asp Asn Asn Asn Asn Asn Asn Ile Ser Ile Asp Asn 485 490 495 His Asn Leu Asn Ile Tyr Asn Asn Ser Ser Glu His Asn Asn Met Cys 500 505 510 Glu Asp Leu Ser Ser Phe Lys Ile Pro Gln Asn Lys Ile Thr Lys Leu 515 520 525 Ile Asn Ser Gly Gln Ser Asn Phe Leu Met Asn Asn Gln Lys Ser Glu 530 535 540 Met Ala Leu Asn Asp Ile Leu Asn Asn Glu Asn Met Met Asn Asn Leu 545 550 555 560 Ile His Glu Lys Gln Lys Leu Asn Ile Asn His Asn Asn Ile Asn Lys 565 570 575 Lys Leu Glu Glu Leu Glu Asn Met Arg Lys Glu Asp Thr Met Phe Leu 580 585 590 Asn Asp Ser Ser Ser Tyr Leu Ser Thr Asn Asp Ile Lys Val Leu Asn 595 600 605 Gly Ile Lys Arg Leu Lys Thr Asp Asn Ser Pro Phe Asn Lys Gln Asp 610 615 620 Glu Glu Phe Leu Leu Asn Ser Lys Asn Leu Asn Lys Tyr Ser Thr Met 625 630 635 640 Gln Asn Asn Lys Ser Tyr Asp Ile Leu Asn Asp Ser Asn Phe Asn Ile 645 650 655 Leu Glu Asn Glu Gly Met Phe Ser Leu Asn Asp Lys Lys Gly Ile Asn 660 665 670 Asn Asn Asp Met Ser Lys Asn Arg Asn Ile Thr Asn Ser Asn Tyr Phe 675 680 685 Lys Pro Phe His Asp Glu Ala Glu Asn Asn Ile Tyr Gln Leu Leu Asn 690 695 700 Gly Lys Tyr Leu Thr Asn Glu Asp Lys Ser Val His Ile Asn Leu Leu 705 710 715 720 Asn Ser Val Met Lys Gln Ser Thr Met Asn Lys Leu Ser Asn Glu Glu 725 730 735 Asp Leu Phe Glu Lys Lys Glu Lys Lys Arg Lys Leu Lys Asn Asn Met 740 745 750 Leu Glu Gln Lys Gly Asp Asp Gln Phe Ile Asn Met Asn Lys Arg Ile 755 760 765 Asp Thr Asn Leu Val Ser Asn Asn Asn Asn Ile Asn Ala Phe Asn Asn 770 775 780 Asn Asn Asn Asn Asn Ile Val Asn Asn Asn Ile Val Asn Asn Asn Ile 785 790 795 800 Val Ser Asn Asn Ile Val Ser Asn Asn Ile Val Ser Asn Asn Ile Ser 805 810 815 Asn Asn Asn Ile Ile Asn Asn Asn Asn Asn Val Ile Pro Phe Gly Asp 820 825 830 Glu His Phe Asp Ala Leu Ser Phe Asp Glu Tyr Arg Lys Arg Ile Asn 835 840 845 Ser Lys Lys Ser Asn Tyr Ser Asn Ile Gly Glu Asn Glu Lys Tyr Lys 850 855 860 Lys Leu Ala Asn Gly Leu Leu Asp Asn Gly Glu Thr Leu Ser Lys Asn 865 870 875 880 Leu Asn Glu Leu Lys Met Tyr His Leu Ala Gly Lys Lys Glu Lys Asp 885 890 895 Asn Asn Met Asn Asn Met Asn Asn Asn Ile Leu Tyr Asn Asn Asn Asn 900 905 910 Met Lys Tyr Tyr Thr His Asn Glu His His Lys Asn Glu Glu Leu Lys 915 920 925 Arg Ser Gln Leu Asn Glu Asn Val Asn His Glu Asn Met Leu Asn Leu 930 935 940 Asn Gln Met Ile Arg Asn Ile Leu Asn Asn Asn Ser Val Asp Asn Asn 945 950 955 960 Glu Lys Ile Ile Lys Gly Ile Asn Ile Leu Lys Asn Met Thr Met Asn 965 970 975 Asp Lys Phe Asn Leu Ile Asn Phe Asn Asn Gln Cys Met Asn Tyr Ser 980 985 990 Tyr Asn Asn Met Met Lys Lys Glu Asn Tyr Val Lys Asn Ile Tyr Lys 995 1000 1005 Ile Ile Asn Lys Gly Ser Glu Asp Ser Ile Asn Tyr Asp Glu Tyr 1010 1015 1020 Leu Thr Val Lys Leu Glu Val Glu Asp Gly Glu Glu Lys Asp Gln 1025 1030 1035 Asn Gly Glu Lys Lys Lys Ile Asn Thr Ser Glu Ala Tyr His Glu 1040 1045 1050 Gly Lys Met Asn Lys Lys His Lys Lys Lys Lys Lys Met Lys Gln 1055 1060 1065 Asn Lys Leu Asp Glu Asp Ile Thr Val Asn Asn Gly Met Ile Asn 1070 1075 1080 Glu Gly Asp Asn Asp Lys Ile Glu Asp Thr Asn Lys Asn Asp Asp 1085 1090 1095 Ile Ser Asn Asn Ser Glu Val Thr Asn Lys Asp Ile Ile Asn Gly 1100 1105 1110 Glu Arg Asn Ser Asn Asp Ser Glu Glu Lys Glu Lys Glu Glu Lys 1115 1120 1125 Glu Asp Tyr Asn Glu Asn Thr Tyr Asn Asn Met Asn Thr Asp Asn 1130 1135 1140 Asn Thr Ser Asn Ser Asn Asp Asp Asn Glu Gln Ile Ile Asn Asn 1145 1150 1155 Glu Asn Asp Tyr Ile Leu Asn Lys Thr Asn Asn Glu Ile Asn Lys 1160 1165 1170 Glu Ile Asn Thr Ser Ile Asn Glu Phe Asn Asp Ile Ala Phe Gln 1175 1180 1185 Leu Cys Pro Trp Lys Lys Gly Ile Gln Trp Asn Leu Leu Lys Lys 1190 1195 1200 Met Trp Thr Cys Lys Leu Trp Asp Asn Lys Gly Asn Glu Ile Thr 1205 1210 1215 Lys Asn Ile His Ile Lys Lys Lys Glu Gly Ile Asp Ile Gly Tyr 1220 1225 1230 Lys Tyr Cys Ile Lys Ile Arg Thr Asn Ser Phe Ile Phe Tyr Leu 1235 1240 1245 Ser Lys Glu Leu Asn Lys Phe Pro His Ile Pro Glu Ile Ser Tyr 1250 1255 1260 Asp Leu Gln Asn Leu His Phe Val Val Ser Tyr Lys Asp Asn Glu 1265 1270 1275 Lys Lys Ile Tyr Ser Phe Glu Asp Gly Ile Tyr Lys Ser Phe Ile 1280 1285 1290 Asp Cys Met Glu Tyr Leu Asn Lys Gln Lys Arg Glu Tyr Asn Glu 1295 1300 1305 Asn Ile Tyr Asp Ile Ser Asn Phe Ile Lys Glu Lys Asn Asn Leu 1310 1315 1320 Glu Leu Asp Asp Ile Tyr Lys Glu Leu Asn Tyr Phe Glu Tyr Asn 1325 1330 1335 Arg Asn Val Leu Ser Asn Ile Cys Glu Glu Thr Lys Ile Ala Tyr 1340 1345 1350 Ser Ile Lys Pro Trp Leu Arg Gly Ile Ile Trp Glu Asp Lys Leu 1355 1360 1365 Lys Lys Trp Ile Val Phe Phe Lys Asp Lys Asn Glu Asn Leu Arg 1370 1375 1380 Ile Ser Phe Phe Ser Thr Ser Asp Tyr Asn Asp Asp Val Ile Met 1385 1390 1395 Ser Tyr Asn Lys Ala Cys Glu Tyr Phe Ser Gln Val Lys Glu Ser 1400 1405 1410 Asn Asn Phe Asp Ser Asn Ser Glu Glu Phe Ala Gln Ser Ile Lys 1415 1420 1425 Asn Val Ile Lys Asn Val Gln Phe Asp Lys Ile Ile Glu Gln Gln 1430 1435 1440 Asn Asp Ala Asn Asn Asn Asp His Lys Lys Gly Asn Leu Gln Glu 1445 1450 1455 Asp His Asp Ile Gln Thr Asn Tyr Tyr Asn Lys Val Thr Gln Gly 1460 1465 1470 Asn Glu Gly Glu Thr Asn Asn Asn Asn Asn Asn Asn Gln Gly Glu 1475 1480 1485 Asn Met Thr Asp Ser Gln Ile Val Leu Asn Thr Asn Glu Ile His 1490 1495 1500 Val His Asn Val His Thr Leu Lys Arg Pro Glu Gly Leu Tyr Asn 1505 1510 1515 Ile Ala Glu Thr Gln Ser Thr His Phe Leu Lys Asp Asn Asn Ile 1520 1525 1530 Ser Asn Glu His Lys Ser Met Glu Thr Phe Tyr Asn Glu Asn Ser 1535 1540 1545 Tyr Tyr Asn Asp Asp Ile Ala Phe Leu Asn Tyr Ala Asp Ser Asn 1550 1555 1560 Asp Ser Leu Ile Ser Lys Gly Met Ser Gln Tyr Leu Ser Leu Pro 1565 1570 1575 Ser Lys Lys Lys Lys Lys Lys Arg Thr Thr Glu Asp Ile Tyr Lys 1580 1585 1590 Ser Glu Ile Asp Val Leu Ser Tyr Gln Asn Asn Glu Leu Tyr Ser 1595 1600 1605 Asn Val Lys Asn Glu Asn Tyr Asp Glu Asn Ile Ser Glu Asn Asn 1610 1615 1620 Lys Lys Tyr Gly Lys Arg Lys Met Glu Leu Asn Phe Leu Ser His 1625 1630 1635 Ser Glu Asn Asn Ile Asn Asn Ile Asn Asn Asn Thr Tyr Asn Asn 1640 1645 1650 Asn Asp Tyr Thr Asn Asp Asp Asn His Ser Ser Lys Val Tyr Ser 1655 1660 1665 Ser Asp Cys Glu Lys Asn Thr Asn His Asn Lys Lys Thr Glu Asp 1670 1675 1680 Lys Tyr Asp Gln Glu Asn Asn Lys Tyr Phe Tyr Asn Lys Asp Lys 1685 1690 1695 Leu Leu Phe Asp His Glu Asn Gly Thr Ile Pro Arg Asn Ile Lys 1700 1705 1710 Lys Glu Ser Asn Asn Leu Asn Ser Glu Tyr Asn Leu Asp Tyr Leu 1715 1720 1725 Asn Ser Glu Asp Ile Lys Asn Asn Asn His His His His Asn Asn 1730 1735 1740 Asn Asn Asn Ile Ile Ile Asn Gly Pro Ser Ser Asp Gly Glu Thr 1745 1750 1755 Ile Ile Leu Ser Gln Tyr His Lys Asn Asp Asp Asp Glu Asp Asp 1760 1765 1770 Gly Asn

Asn Thr Asp Asn Ser Lys Ile Leu Arg Ser Phe Asp Lys 1775 1780 1785 Cys Ser Asn Glu Asn Ser Val Cys Ile Asn Lys Ser Asn Glu Lys 1790 1795 1800 Leu Glu Asn Ile Leu Asp Gly Gly Val Asn Met Arg Leu Pro Lys 1805 1810 1815 Ser Glu Ile Leu Asn Gln Ala Ser Arg Leu Pro Lys Leu Gln Gly 1820 1825 1830 Met Phe Phe Asp Lys Arg Arg Asn Tyr Trp Thr Val Ser Val Cys 1835 1840 1845 Gly Phe Arg Lys Ser Phe Gly Val Arg Thr Arg Gly Val Tyr Gln 1850 1855 1860 Ala Tyr Lys Leu Ala Ala Glu Phe Arg Asn Arg Ile Leu Glu Asn 1865 1870 1875 Asn Lys Gly Lys Tyr Tyr Pro Gln Arg Ser Thr Ser Met Ser Ser 1880 1885 1890 Asn Tyr Lys Tyr Asn Thr Lys Gly Gly Ser Ile Ile Ile Lys Asp 1895 1900 1905 Glu Lys Leu Asn Tyr Ser Asn Ile Ser Glu Met Thr Ser Val Asp 1910 1915 1920 Glu Glu Tyr Asp Pro Lys Lys Lys Lys Ser Ser Phe Ser Ser Asn 1925 1930 1935 Asn Ile Asn Asn Ile Asp Lys Glu Leu Tyr Ser Leu Asn Asp Lys 1940 1945 1950 Glu Ser Asn Ser Ser Gly Leu Tyr Tyr Ser Phe Asn Val Lys Gly 1955 1960 1965 Lys Thr Ser Ser Asn Ile Tyr Asp Tyr Phe Leu Asp Lys Lys Glu 1970 1975 1980 His Asp Tyr Phe Ala Thr Asn Asn Ser Ser Asn Asn Ile Asn Asn 1985 1990 1995 Asn Asn Asn Asn Asn Asn Asn Asp Ile Asn Asn Asn Ser Thr Asn 2000 2005 2010 Asp Ile Asn Asn Asn Gly Thr Asn Asp Thr Asn Asn Lys Lys Asp 2015 2020 2025 Pro Lys Gly Ile Pro Asn Asn Ser Cys Tyr Asp Asp Gln Tyr Asn 2030 2035 2040 Asn Ile Glu Asn Glu Glu Glu Glu Glu Glu Arg Glu Gly Glu Glu 2045 2050 2055 Ala Glu Arg Trp Asn Ile Ser Asp Lys Val Asn Asn Lys Asn Asn 2060 2065 2070 Asp Asn Lys Ile Gln Ile Gly Asn Asn Thr Phe Gly Gln Leu Lys 2075 2080 2085 Tyr Asp Ser Ala Leu Glu Ser Asn Leu Ile Asn Phe Ile Asn Glu 2090 2095 2100 Val Gly Ser Ser Asp Phe Leu Lys Asp Ala Lys Ile Asn Asn Asn 2105 2110 2115 Asn Asn Asn Asn Asn Asn Asn Asn Asn Ile Gly Gly Asn Lys Lys 2120 2125 2130 Asn Gln His Ile Asn Asn Asn Ile Ser Asn Ser Asn Thr Leu Asn 2135 2140 2145 Asn Ser Leu Asn Val Gln Asn Ile Ser Ser Ser Asn Ser Ala Ile 2150 2155 2160 Ser Asn Asn Gly Val Ile Asn Asn Tyr Ser Pro Thr His Ile Tyr 2165 2170 2175 Glu Val Asp Asn Leu Asn Asn Ser Gln Asp Tyr Tyr Leu Lys Asn 2180 2185 2190 Asp Glu Met Lys Asn Glu Leu Asn Lys Val Glu Asp Gln Lys Asn 2195 2200 2205 Lys Lys Asp Ile Ser Ser Thr Asn Ser Leu Leu Lys His Phe Met 2210 2215 2220 Asn Asp Asp Ile Leu Ile Asn Asn Lys Val Lys Gln Gln Pro Phe 2225 2230 2235 Phe Asp Ser His Asn Asn Tyr Asn Lys Leu Gly His Lys Gln Val 2240 2245 2250 Lys Thr Ser Ala Tyr Thr Glu Ser Ala Ile Gly Leu Gly Phe Arg 2255 2260 2265 Lys Asn Gly Tyr Ile Asn Asp Cys Asp Leu Val Asn Asn Asn Asn 2270 2275 2280 Asn Asn Asn Asn Asn Asn Met His Ile Lys Asn Asn Gly Val Ile 2285 2290 2295 Leu Asn Lys Leu Ile Ser Ser Asn His Asp Ser Lys Asp Asn Ser 2300 2305 2310 Asn Asn Asn Ile Lys Asp Lys Asp Arg Glu Asp Asn Asn Lys Asn 2315 2320 2325 Ile Tyr Asn Asn Asn Asn Asp Arg Ser Ile Gly Ser Tyr Val Asn 2330 2335 2340 Asn Leu Gly Val Ile Glu Asn Gln Cys Glu Asn Ser Lys Gly Ala 2345 2350 2355 Asp Glu Asn Thr Lys Asn Phe Asn Asn Asn Lys Thr Leu Asn Asn 2360 2365 2370 Tyr Glu Pro Glu Asn Lys Ser Tyr Asn Tyr Ser Pro Val Leu Asp 2375 2380 2385 Glu Thr Leu Glu Glu Arg Asp Val Arg Ile Asn Arg Glu Val Ser 2390 2395 2400 Lys Cys Lys Phe Val Asp Gly Leu Ile Tyr Asp Glu Ala Asn Lys 2405 2410 2415 Cys Phe Arg Ile Lys Ile Asn Gly Tyr Arg Lys Ala Tyr Ser Val 2420 2425 2430 Ile Arg Arg Gly Val Lys Glu Ala Tyr Lys Leu Ser Ile Glu Ser 2435 2440 2445 Ile Glu Gln Ile Lys Arg Gln Thr Gln Thr Asn Asn Phe Asn Asn 2450 2455 2460 Asn Glu Gln Ser Gln Val Asn Thr Asn Asn Met Thr Gln Ile Tyr 2465 2470 2475 Asn Ser Ser Ser Glu Asn Ser Arg His Gly Ser Cys Tyr Asn Tyr 2480 2485 2490 Gln His Asn His Lys Ser Lys Leu Glu Asn Ser Asn Asp Asn Pro 2495 2500 2505 Glu Glu Lys Tyr Asp His Ile Asn Asn Met Thr Asn Val Gly His 2510 2515 2520 Glu Asn Lys Gln Ser Ala Glu Glu Ser Leu Phe Pro Met Leu Asn 2525 2530 2535 Val Asp Asp Lys Tyr Tyr Glu Leu Leu Lys Thr Ser Ile Ile Ile 2540 2545 2550 Cys Leu Asn Asp Ile Leu Met Asn Cys Ile Pro Gln Val Phe His 2555 2560 2565 Leu Tyr Lys Asn Ile Asn Thr Ser Asn Asp Ile Lys Leu Glu Asp 2570 2575 2580 Ile Leu Tyr Thr Glu Arg Lys Arg Lys Glu Gln Ser Leu Lys Tyr 2585 2590 2595 His Ile Glu Tyr Thr Gln Asn Ser Val Gly Val Ser Ser Leu Ile 2600 2605 2610 Pro Tyr Leu Lys Leu Phe Ser Thr Glu Ile Leu Asn Asn Val Leu 2615 2620 2625 Pro Ser Ala Gln Ser Leu Glu Ile Gln Arg Leu Ile Ile His Ser 2630 2635 2640 Leu Asp Ile Gln Thr Tyr Asn Thr Leu Tyr 2645 2650 311725DNAPlasmodium falciparum 31atgaatcatc ttgggaatgt taaatattta gtcattgtgt ttttgatttt ctttgatttg 60tttctagtta atggtagaga tgtgcaaaac aatatagtgg atgaaataaa atatcgtgaa 120gaagtatgta atgatgaggt agatctttac cttctaatgg attgttctgg aagtatacgt 180cgtcataatt gggtgaacca tgcagtacct ctagctatga aattgataca acaattaaat 240cttaatgata atgcaattca cttatatgct agtgtttttt caaacaatgc aagagaaatt 300attagattac atagtgatgc atctaaaaac aaagagaagg ctttaattat tataaagtca 360ctcttaagta caaatcttcc atatggtaaa acaaacttaa ctgatgcact gttacaagta 420agaaaacatt taaatgaccg aatcaataga gagaatgcta atcaattagt tgttatatta 480acagatggaa ttccagatag tattcaagat tcattaaaag aatcaagaaa attaagtgat 540cgtggtgtta aaatagctgt ttttggtatt ggacaaggta ttaatgtagc tttcaacaga 600tttcttgtag gttgtcatcc atcagatggt aaatgtaact tgtatgctga ttctgcatgg 660gaaaatgtaa aaaatgttat cggacccttt atgaaggctg tttgtgttga agtagaaaaa 720acagcaagtt gtggtgtttg ggacgaatgg tctccatgta gtgtaacttg tggtaaaggt 780accaggtcaa gaaaaagaga aatcttacac gaaggatgta caagtgaatt acaagaacaa 840tgtgaagaag aaagatgtct tccaaaacgg gaaccattag atgttccaga tgaacccgaa 900gatgatcaac ctagaccaag aggagataat tttgctgtcg aaaaaccaaa cgaaaatata 960atagataata atccacaaga accttcacca aatccagaag aaggaaaggg tgaaaatcca 1020aacggatttg atttagatga aaatccagaa aatccaccaa atccaccaaa tccaccaaat 1080ccaccaaatc caccaaatcc accaaatcca gatattcctg aacaagaacc aaatatacct 1140gaagattcag aaaaagaagt accttctgat gttccaaaaa atccagaaga cgatcgagaa 1200gaaaactttg atattccaaa gaaacccgaa aataagcacg ataatcaaaa taatttacca 1260aatgataaaa gtgatagata tattccatat tcaccattat ctccaaaagt tttggataat 1320gaaaggaaac aaagtgaccc ccaaagtcaa gataataatg gaaataggca cgtacctaat 1380agtgaagata gagaaacacg tccacatggt agaaataatg aaaatagatc atacaataga 1440aaacataaca atactccaaa acatcctgaa agggaagaac atgaaaagcc agataataat 1500aaaaaaaaag caggatcaga taataaatat aaaattgcag gtggaatagc tggaggatta 1560gctttactcg catgtgctgg acttgcttat aaattcgtag taccaggagc agcaacaccc 1620tatgccggag aacctgcacc ttttgatgaa acattaggtg aagaagataa agatttggac 1680gaacctgaac aattcagatt acctgaagaa aacgagtgga attaa 172532574PRTPlasmodium falciparum 32Met Asn His Leu Gly Asn Val Lys Tyr Leu Val Ile Val Phe Leu Ile 1 5 10 15 Phe Phe Asp Leu Phe Leu Val Asn Gly Arg Asp Val Gln Asn Asn Ile 20 25 30 Val Asp Glu Ile Lys Tyr Arg Glu Glu Val Cys Asn Asp Glu Val Asp 35 40 45 Leu Tyr Leu Leu Met Asp Cys Ser Gly Ser Ile Arg Arg His Asn Trp 50 55 60 Val Asn His Ala Val Pro Leu Ala Met Lys Leu Ile Gln Gln Leu Asn 65 70 75 80 Leu Asn Asp Asn Ala Ile His Leu Tyr Ala Ser Val Phe Ser Asn Asn 85 90 95 Ala Arg Glu Ile Ile Arg Leu His Ser Asp Ala Ser Lys Asn Lys Glu 100 105 110 Lys Ala Leu Ile Ile Ile Lys Ser Leu Leu Ser Thr Asn Leu Pro Tyr 115 120 125 Gly Lys Thr Asn Leu Thr Asp Ala Leu Leu Gln Val Arg Lys His Leu 130 135 140 Asn Asp Arg Ile Asn Arg Glu Asn Ala Asn Gln Leu Val Val Ile Leu 145 150 155 160 Thr Asp Gly Ile Pro Asp Ser Ile Gln Asp Ser Leu Lys Glu Ser Arg 165 170 175 Lys Leu Ser Asp Arg Gly Val Lys Ile Ala Val Phe Gly Ile Gly Gln 180 185 190 Gly Ile Asn Val Ala Phe Asn Arg Phe Leu Val Gly Cys His Pro Ser 195 200 205 Asp Gly Lys Cys Asn Leu Tyr Ala Asp Ser Ala Trp Glu Asn Val Lys 210 215 220 Asn Val Ile Gly Pro Phe Met Lys Ala Val Cys Val Glu Val Glu Lys 225 230 235 240 Thr Ala Ser Cys Gly Val Trp Asp Glu Trp Ser Pro Cys Ser Val Thr 245 250 255 Cys Gly Lys Gly Thr Arg Ser Arg Lys Arg Glu Ile Leu His Glu Gly 260 265 270 Cys Thr Ser Glu Leu Gln Glu Gln Cys Glu Glu Glu Arg Cys Leu Pro 275 280 285 Lys Arg Glu Pro Leu Asp Val Pro Asp Glu Pro Glu Asp Asp Gln Pro 290 295 300 Arg Pro Arg Gly Asp Asn Phe Ala Val Glu Lys Pro Asn Glu Asn Ile 305 310 315 320 Ile Asp Asn Asn Pro Gln Glu Pro Ser Pro Asn Pro Glu Glu Gly Lys 325 330 335 Gly Glu Asn Pro Asn Gly Phe Asp Leu Asp Glu Asn Pro Glu Asn Pro 340 345 350 Pro Asn Pro Pro Asn Pro Pro Asn Pro Pro Asn Pro Pro Asn Pro Pro 355 360 365 Asn Pro Asp Ile Pro Glu Gln Glu Pro Asn Ile Pro Glu Asp Ser Glu 370 375 380 Lys Glu Val Pro Ser Asp Val Pro Lys Asn Pro Glu Asp Asp Arg Glu 385 390 395 400 Glu Asn Phe Asp Ile Pro Lys Lys Pro Glu Asn Lys His Asp Asn Gln 405 410 415 Asn Asn Leu Pro Asn Asp Lys Ser Asp Arg Tyr Ile Pro Tyr Ser Pro 420 425 430 Leu Ser Pro Lys Val Leu Asp Asn Glu Arg Lys Gln Ser Asp Pro Gln 435 440 445 Ser Gln Asp Asn Asn Gly Asn Arg His Val Pro Asn Ser Glu Asp Arg 450 455 460 Glu Thr Arg Pro His Gly Arg Asn Asn Glu Asn Arg Ser Tyr Asn Arg 465 470 475 480 Lys His Asn Asn Thr Pro Lys His Pro Glu Arg Glu Glu His Glu Lys 485 490 495 Pro Asp Asn Asn Lys Lys Lys Ala Gly Ser Asp Asn Lys Tyr Lys Ile 500 505 510 Ala Gly Gly Ile Ala Gly Gly Leu Ala Leu Leu Ala Cys Ala Gly Leu 515 520 525 Ala Tyr Lys Phe Val Val Pro Gly Ala Ala Thr Pro Tyr Ala Gly Glu 530 535 540 Pro Ala Pro Phe Asp Glu Thr Leu Gly Glu Glu Asp Lys Asp Leu Asp 545 550 555 560 Glu Pro Glu Gln Phe Arg Leu Pro Glu Glu Asn Glu Trp Asn 565 570 336648DNAPlasmodium falciparum 33atgaataaat attggttgta tattgcttat gtttatctat tattaaacat actatcaaaa 60tgtaataaaa tagaaaataa taataataag gtcaagaatt tgaccgttta caataatgat 120ataggacaat attttaaaaa aaaggacata caatgcaaac accatataga aataaatcaa 180gactctaatc ataatgaata ttccttttta tcattaaagg caagttcaat atttaaccag 240tactatgttg ccaaattaat taataccctt ttatatagag gatttcattt gaatgtctat 300ttccataaaa atgtggctat gtatgaatcc ttttccagaa cgaacttttt cttttattta 360accatattaa acaaaaaaaa tgtcaaaaaa attgttaaga taatatccaa agcagaacgt 420tcaagtaata aaagaaagtt taatgtattc aagaagtatt taatttctga gtttgattat 480cccaattttg aaatcgacga caccgtaaaa aatgaaatga accaagcaat tttagtatat 540aaaaaagcaa aatctgattc atactggagt gttatggatg cattaaagaa ggatggttta 600ttattagcta gaaccttttt atctgtttct tttgttcaaa gtcttagagg tattatcggt 660cttattaacc acaaattaat agatttatgt tttactaatg cctatgtatt taaccacgta 720gcatcttttg ataaacttat tatgaataat atttttggtg ttataatgtc atatgtattt 780aaatcacttt tactcttttt ctacccatta attatacctt tccgtggtgc atttgccttt 840gctatatctg ctttttgtat cacacagttg ggaaaaatag tatttgcaat atacaaaaat 900ctcagacaat tatatagaat atcatacaga aaaatataca gtattgtatt aaaagttaaa 960ttaagaaatg aaccagaact taaaaaatat gccatgaaat tattatacgg tgatgcttta 1020attatgatta caaaaatatg gaagttgtca tatgtaaatg tcagtgagca cttgaatgga 1080aaaaatgttt atccaatttt aaataactta tttgaaaaaa atttgggaac aggtttcttt 1140gattttagta actccttatt caaatatgtt atgaattact tagaggaaat aaatttatta 1200aatgccaaaa gtgttgatgt agaaaaagaa ctcattttga acagacataa tttcaaacta 1260ttacttaaaa ttttaaagat tactaaaaag tccttattat atgaagagtc ttatatgaag 1320atatctgttg ctaatttatt aacaaaattt tacaccttga ttttagtaaa tatagaacat 1380ataagtaaat taaatccaaa ggaacatttc tataatgatt tagataatga atttaaacat 1440atatatcaag atcaaatgtt tgaattattt atacaaaaga tatcttcaga tattgttaga 1500aaaccattta tcaaaagaaa tatcggaaga atagataaag gtagtataga attaacatta 1560gcattaataa aagtaaaatt attacattac aaaccaacat tgaataatcc atattccagt 1620ttatattttg atgagaattt aaaaaaacaa ttaaattatg catttaaatt aattattatt 1680ggttctacta gtataattgc tagtcttgca aactacggag aaagatatgg tgttttaaaa 1740caatgtccat tagatattgt taagaattta aatcaacaat gtgaatatgt ttcttttgaa 1800attaagaaat taatattccc aataaatatt tttgtgaact tattaattcc attattttta 1860ccatatgatg atgtattagt agataaaaat ataattgatg aagtcaaaaa attctttaac 1920gttattatcg ataccgatga ttcttactta caaaaatata tgaaaacaac aattaacact 1980ataagaaaat caaaagatct agatataaat aatgtaaatt atgaagaaga aatggaaaaa 2040attgtagttc aagaagcaca taaagttatt gaagaaatta acaaagaaag acgagcatct 2100ttcaaatttc aagattttgt agtcaaagaa gattcagtag tattgtataa taaaagtgga 2160atccaatatt catttaaatt tgatcattta aatagaaaag aagattttat gcgtattata 2220ggtagttatc aatttaaaaa tcccataggt tatcaaacat cacaacttgt ttttgaacct 2280caaaataatc atattggttc tttaatggtt ggatcaaata aaaatacatt cgatggaaaa 2340ttaagtgtat gtcatatgtg ttataaagaa gatgatgatt ctttggttgt tgatgcaata 2400tttagtatcc tctggtcttc tggaattgat agatttagtg cctttatatt cgcttccttc 2460atcggtgctg tgaaacaaac ttatcaccaa ggtacctcct ggaaacgtgc tctcagtaat 2520atggccccaa ccgaatttta cgaaatgcac aaaattttta tggataagag tgtttacgga 2580aaagaaaaaa gtcaaaatta cttcttgaaa aacataagaa aatacagatt tcaatttagt 2640agaggctcat tctctagaat gtttagaacg ttcttagaaa attctttaaa taaaattaat 2700ttctttaact cagaagaagc catcattatt ttagttatgt caaccttata tgctctttac 2760aaaaatattg aaaaattcga tattccatta aaagaaacct ataaattata tcaacagaaa 2820ttaattgaat cttattacca tgtagacaaa tattcacacc attatgaaac atatactcta 2880aatattagaa gaaaaaaata caatgcactt gtcaagagtg aacatgaaaa ggcacaagaa 2940gctagccaag gagaaggtaa agacgaagca gaaaatgctg aattaaaaaa aagagttgaa 3000aagagaatta actttaatga tatacaatta actatggaag atgaagttgt aaggaataca 3060aaatatttac aattagaatt acaagcaaaa ccacaagaaa gagaaaaaat catacaatat 3120ttaacatata aatataagga aataccaaat catgaaatgt tcccacattt accacaccaa 3180tgttatttct tattatatta taattatgaa ccctttttag aaaaaaacct tcatggtcta 3240gaaggtgttg ttagtaaaat tacacgaaaa tcagtcttaa gtagatactt aaaaaatatt 3300aatttaatac aaccagagaa aacgagattt gcatctataa aattaaaaga tttaatcaat 3360atactttgtg gtacacacat gtttttgaaa aaagaacata tcacatttga tgaaatatat 3420aaatatgaaa attcaaatga tgctttgaaa

catcttctta tcattgttgc attattaaga 3480attgaaaaga gaactaaccg atattcttgg acaacatatt taactttaca aaaacaatta 3540gatgaaagaa aaagatatta tgatacacca tttaaatatt tatttttaaa aataagcaaa 3600gtaagaagat tttatggaag cgcaaaaaaa aaaatgttat taggatttgg taaaaaattt 3660aaaggagtta gatttttaaa tatattaaga tataccagat tctttgaaat tatggaatat 3720gctgaatcaa ttaatgaatt ttatccatat tgttatatta aatttgaaga tgtattaaca 3780ttctcaaatg tattttataa atttaaatat tctcttttaa gatatacaac aagtaaacaa 3840tctgttagaa atgttgtgaa caatttcaat ttgtctccac aaacaacagg aaacaatgaa 3900aacttattat taagaatata tagatttatt gaattaatta ttaaaaagaa gtataatgtt 3960gatatacagc atgttagaaa agatgataaa gattttaata gacatgcata taataagaat 4020gaatatatat taaatgatat aaagtttaat cctaatattg aacaatattt aagacaatta 4080actggtttca ttaaactttt aacagatatg aaatttacta atttcttatt cttaagaaac 4140ttatattact ttgttaaatt ttatgcagta acgggtgatt taacttattc aataaataat 4200tcttctatat atttatgtag tagaaattat ttagaattta tattaaattc cattattgaa 4260tttgaaaatt ttaaaaaatt tatggttaag attaaagaaa aatttgatat tgaaaaatat 4320ccaattgatc catataattt ccaaacagaa tgttattcta tagatagtgt caaaacatat 4380agattctttt taagtgattt agataaatta aaatcatatc aagatataga aaacttacag 4440aatgtctcat cattttataa agattactta tatatgggat tattttatga aaacttagat 4500gtcagaaatt taaatgtata ttataatttc aataaaaaaa aaaatcaaga tcataaagca 4560catggatcaa atgtattcct tgatgggggt ctttcattaa gtaaaaatat gtcatttagt 4620gatataaatg atttaagtga atctattcaa aattatttat atttagaaaa atttttagct 4680gatagtaata taccttctat tccatatcaa ggattttcgg ttagacgtgt aaatgatggt 4740atgcatgtaa catataataa tagatcaact acaccaccac tttataaaga taatgtttta 4800gatcaattcg aattagtagg aaaatcatta attagtgaat atttccaaaa agtcaaatgt 4860tcatttattc aatatccatt caatttatat tattggctag tccttaatcc aggaaaacca 4920aatataagct taagtagtaa aaccggtcta attaaagaag atgatttaat acctaaaacc 4980gttgaagaaa aattaaagga agaagaagaa catgatatta aaattgttga aaatatatta 5040tctgaagata tctttgattt taaagataaa tcagatgatg atcaaagtac aaccgctgat 5100gtactttcta cagacacaga tgattcagaa actgacgctg aaaaaacatc aaacaattca 5160aataccctac acaaaaaaga aacaccagca atgaaatata atatgaatat agcacaagat 5220gaaattaata gacaaaatga tgtatctaaa aatacaacat atgcagaaaa taatgaatac 5280acatctgaaa atataactaa accatcagat caaaatacag aaaactatgg aacaaaaatg 5340ccctctccat cattcataca aatagataaa gttaaccaac aagatcaaca aaatgatgct 5400catatagaaa gttctaaaaa tactcaagaa aatcttaact atgatgataa cactaatatg 5460tatgaagaaa ataaaaatga taagaaaaac aaaaaattaa ataataatac aaaagaaata 5520agtttccttg aaagaagaga aactaatcct ataccattta gttcaaatat gattaatgaa 5580aaaaataaag aagctcataa agaacactat ggaaatttca atagaccata taatatatat 5640gtacctaaaa aagatatctt cagaaatttc catctagttg taaaagtagt acatgcatta 5700atatccttaa ataaattctc catggttagt ccagatacaa tattaaaaaa aggtatagaa 5760tccatgaaaa aaaaacactc actcaaaatg attaaaaata taaatccatt tatacataaa 5820atgatacttg acatgaatga aaccatacaa aaattaaaat ccgatagtga aaaagcatat 5880ggtggaccaa aagcagatat tatatcttta tataaaattg tggaattcca attatttaat 5940caatatatta tatatccacc attaaaaaga ttaaccaaaa aagaacttaa atatatactt 6000gatgttgtta accaaggata ttatttctac ctaaaaaatg ttatcacaaa attaaaaaga 6060gaaaatctac aaaaatcgaa agttgtcaaa atatttaata atttccaaga attctctaat 6120ttattagatg atgatggtgt agataaatta tataatatat ttactgaaac atttaaatgt 6180aaaaatatta aatgttttga tcttttattc caaggattct taaccgaaca atataataac 6240attgtctatc gttatacaca tgatcatgat gctatgaact catataatga taacatattt 6300aataataaag aattattaaa caaaatattt agaagagcat atgacaacta ttttattaca 6360cctgttaaaa acaatcaacc aaaaaaactc atatctttag aaaaaccatc attagaacaa 6420ccagtattga cgttagaaga atttgcatat ggattccatg aatttggaaa taaaattgaa 6480aaatggagat caatgcttac cctattcaat ataagacata tatatattaa tatatgccac 6540atgctattaa gtgttaaaca tagtatacaa cgatcacaca gagtttttat gcacaaattt 6600ggtttcttcg gtatcttcag aagaaagagg gaaatttaca taccataa 6648342215PRTPlasmodium falciparum 34Met Asn Lys Tyr Trp Leu Tyr Ile Ala Tyr Val Tyr Leu Leu Leu Asn 1 5 10 15 Ile Leu Ser Lys Cys Asn Lys Ile Glu Asn Asn Asn Asn Lys Val Lys 20 25 30 Asn Leu Thr Val Tyr Asn Asn Asp Ile Gly Gln Tyr Phe Lys Lys Lys 35 40 45 Asp Ile Gln Cys Lys His His Ile Glu Ile Asn Gln Asp Ser Asn His 50 55 60 Asn Glu Tyr Ser Phe Leu Ser Leu Lys Ala Ser Ser Ile Phe Asn Gln 65 70 75 80 Tyr Tyr Val Ala Lys Leu Ile Asn Thr Leu Leu Tyr Arg Gly Phe His 85 90 95 Leu Asn Val Tyr Phe His Lys Asn Val Ala Met Tyr Glu Ser Phe Ser 100 105 110 Arg Thr Asn Phe Phe Phe Tyr Leu Thr Ile Leu Asn Lys Lys Asn Val 115 120 125 Lys Lys Ile Val Lys Ile Ile Ser Lys Ala Glu Arg Ser Ser Asn Lys 130 135 140 Arg Lys Phe Asn Val Phe Lys Lys Tyr Leu Ile Ser Glu Phe Asp Tyr 145 150 155 160 Pro Asn Phe Glu Ile Asp Asp Thr Val Lys Asn Glu Met Asn Gln Ala 165 170 175 Ile Leu Val Tyr Lys Lys Ala Lys Ser Asp Ser Tyr Trp Ser Val Met 180 185 190 Asp Ala Leu Lys Lys Asp Gly Leu Leu Leu Ala Arg Thr Phe Leu Ser 195 200 205 Val Ser Phe Val Gln Ser Leu Arg Gly Ile Ile Gly Leu Ile Asn His 210 215 220 Lys Leu Ile Asp Leu Cys Phe Thr Asn Ala Tyr Val Phe Asn His Val 225 230 235 240 Ala Ser Phe Asp Lys Leu Ile Met Asn Asn Ile Phe Gly Val Ile Met 245 250 255 Ser Tyr Val Phe Lys Ser Leu Leu Leu Phe Phe Tyr Pro Leu Ile Ile 260 265 270 Pro Phe Arg Gly Ala Phe Ala Phe Ala Ile Ser Ala Phe Cys Ile Thr 275 280 285 Gln Leu Gly Lys Ile Val Phe Ala Ile Tyr Lys Asn Leu Arg Gln Leu 290 295 300 Tyr Arg Ile Ser Tyr Arg Lys Ile Tyr Ser Ile Val Leu Lys Val Lys 305 310 315 320 Leu Arg Asn Glu Pro Glu Leu Lys Lys Tyr Ala Met Lys Leu Leu Tyr 325 330 335 Gly Asp Ala Leu Ile Met Ile Thr Lys Ile Trp Lys Leu Ser Tyr Val 340 345 350 Asn Val Ser Glu His Leu Asn Gly Lys Asn Val Tyr Pro Ile Leu Asn 355 360 365 Asn Leu Phe Glu Lys Asn Leu Gly Thr Gly Phe Phe Asp Phe Ser Asn 370 375 380 Ser Leu Phe Lys Tyr Val Met Asn Tyr Leu Glu Glu Ile Asn Leu Leu 385 390 395 400 Asn Ala Lys Ser Val Asp Val Glu Lys Glu Leu Ile Leu Asn Arg His 405 410 415 Asn Phe Lys Leu Leu Leu Lys Ile Leu Lys Ile Thr Lys Lys Ser Leu 420 425 430 Leu Tyr Glu Glu Ser Tyr Met Lys Ile Ser Val Ala Asn Leu Leu Thr 435 440 445 Lys Phe Tyr Thr Leu Ile Leu Val Asn Ile Glu His Ile Ser Lys Leu 450 455 460 Asn Pro Lys Glu His Phe Tyr Asn Asp Leu Asp Asn Glu Phe Lys His 465 470 475 480 Ile Tyr Gln Asp Gln Met Phe Glu Leu Phe Ile Gln Lys Ile Ser Ser 485 490 495 Asp Ile Val Arg Lys Pro Phe Ile Lys Arg Asn Ile Gly Arg Ile Asp 500 505 510 Lys Gly Ser Ile Glu Leu Thr Leu Ala Leu Ile Lys Val Lys Leu Leu 515 520 525 His Tyr Lys Pro Thr Leu Asn Asn Pro Tyr Ser Ser Leu Tyr Phe Asp 530 535 540 Glu Asn Leu Lys Lys Gln Leu Asn Tyr Ala Phe Lys Leu Ile Ile Ile 545 550 555 560 Gly Ser Thr Ser Ile Ile Ala Ser Leu Ala Asn Tyr Gly Glu Arg Tyr 565 570 575 Gly Val Leu Lys Gln Cys Pro Leu Asp Ile Val Lys Asn Leu Asn Gln 580 585 590 Gln Cys Glu Tyr Val Ser Phe Glu Ile Lys Lys Leu Ile Phe Pro Ile 595 600 605 Asn Ile Phe Val Asn Leu Leu Ile Pro Leu Phe Leu Pro Tyr Asp Asp 610 615 620 Val Leu Val Asp Lys Asn Ile Ile Asp Glu Val Lys Lys Phe Phe Asn 625 630 635 640 Val Ile Ile Asp Thr Asp Asp Ser Tyr Leu Gln Lys Tyr Met Lys Thr 645 650 655 Thr Ile Asn Thr Ile Arg Lys Ser Lys Asp Leu Asp Ile Asn Asn Val 660 665 670 Asn Tyr Glu Glu Glu Met Glu Lys Ile Val Val Gln Glu Ala His Lys 675 680 685 Val Ile Glu Glu Ile Asn Lys Glu Arg Arg Ala Ser Phe Lys Phe Gln 690 695 700 Asp Phe Val Val Lys Glu Asp Ser Val Val Leu Tyr Asn Lys Ser Gly 705 710 715 720 Ile Gln Tyr Ser Phe Lys Phe Asp His Leu Asn Arg Lys Glu Asp Phe 725 730 735 Met Arg Ile Ile Gly Ser Tyr Gln Phe Lys Asn Pro Ile Gly Tyr Gln 740 745 750 Thr Ser Gln Leu Val Phe Glu Pro Gln Asn Asn His Ile Gly Ser Leu 755 760 765 Met Val Gly Ser Asn Lys Asn Thr Phe Asp Gly Lys Leu Ser Val Cys 770 775 780 His Met Cys Tyr Lys Glu Asp Asp Asp Ser Leu Val Val Asp Ala Ile 785 790 795 800 Phe Ser Ile Leu Trp Ser Ser Gly Ile Asp Arg Phe Ser Ala Phe Ile 805 810 815 Phe Ala Ser Phe Ile Gly Ala Val Lys Gln Thr Tyr His Gln Gly Thr 820 825 830 Ser Trp Lys Arg Ala Leu Ser Asn Met Ala Pro Thr Glu Phe Tyr Glu 835 840 845 Met His Lys Ile Phe Met Asp Lys Ser Val Tyr Gly Lys Glu Lys Ser 850 855 860 Gln Asn Tyr Phe Leu Lys Asn Ile Arg Lys Tyr Arg Phe Gln Phe Ser 865 870 875 880 Arg Gly Ser Phe Ser Arg Met Phe Arg Thr Phe Leu Glu Asn Ser Leu 885 890 895 Asn Lys Ile Asn Phe Phe Asn Ser Glu Glu Ala Ile Ile Ile Leu Val 900 905 910 Met Ser Thr Leu Tyr Ala Leu Tyr Lys Asn Ile Glu Lys Phe Asp Ile 915 920 925 Pro Leu Lys Glu Thr Tyr Lys Leu Tyr Gln Gln Lys Leu Ile Glu Ser 930 935 940 Tyr Tyr His Val Asp Lys Tyr Ser His His Tyr Glu Thr Tyr Thr Leu 945 950 955 960 Asn Ile Arg Arg Lys Lys Tyr Asn Ala Leu Val Lys Ser Glu His Glu 965 970 975 Lys Ala Gln Glu Ala Ser Gln Gly Glu Gly Lys Asp Glu Ala Glu Asn 980 985 990 Ala Glu Leu Lys Lys Arg Val Glu Lys Arg Ile Asn Phe Asn Asp Ile 995 1000 1005 Gln Leu Thr Met Glu Asp Glu Val Val Arg Asn Thr Lys Tyr Leu 1010 1015 1020 Gln Leu Glu Leu Gln Ala Lys Pro Gln Glu Arg Glu Lys Ile Ile 1025 1030 1035 Gln Tyr Leu Thr Tyr Lys Tyr Lys Glu Ile Pro Asn His Glu Met 1040 1045 1050 Phe Pro His Leu Pro His Gln Cys Tyr Phe Leu Leu Tyr Tyr Asn 1055 1060 1065 Tyr Glu Pro Phe Leu Glu Lys Asn Leu His Gly Leu Glu Gly Val 1070 1075 1080 Val Ser Lys Ile Thr Arg Lys Ser Val Leu Ser Arg Tyr Leu Lys 1085 1090 1095 Asn Ile Asn Leu Ile Gln Pro Glu Lys Thr Arg Phe Ala Ser Ile 1100 1105 1110 Lys Leu Lys Asp Leu Ile Asn Ile Leu Cys Gly Thr His Met Phe 1115 1120 1125 Leu Lys Lys Glu His Ile Thr Phe Asp Glu Ile Tyr Lys Tyr Glu 1130 1135 1140 Asn Ser Asn Asp Ala Leu Lys His Leu Leu Ile Ile Val Ala Leu 1145 1150 1155 Leu Arg Ile Glu Lys Arg Thr Asn Arg Tyr Ser Trp Thr Thr Tyr 1160 1165 1170 Leu Thr Leu Gln Lys Gln Leu Asp Glu Arg Lys Arg Tyr Tyr Asp 1175 1180 1185 Thr Pro Phe Lys Tyr Leu Phe Leu Lys Ile Ser Lys Val Arg Arg 1190 1195 1200 Phe Tyr Gly Ser Ala Lys Lys Lys Met Leu Leu Gly Phe Gly Lys 1205 1210 1215 Lys Phe Lys Gly Val Arg Phe Leu Asn Ile Leu Arg Tyr Thr Arg 1220 1225 1230 Phe Phe Glu Ile Met Glu Tyr Ala Glu Ser Ile Asn Glu Phe Tyr 1235 1240 1245 Pro Tyr Cys Tyr Ile Lys Phe Glu Asp Val Leu Thr Phe Ser Asn 1250 1255 1260 Val Phe Tyr Lys Phe Lys Tyr Ser Leu Leu Arg Tyr Thr Thr Ser 1265 1270 1275 Lys Gln Ser Val Arg Asn Val Val Asn Asn Phe Asn Leu Ser Pro 1280 1285 1290 Gln Thr Thr Gly Asn Asn Glu Asn Leu Leu Leu Arg Ile Tyr Arg 1295 1300 1305 Phe Ile Glu Leu Ile Ile Lys Lys Lys Tyr Asn Val Asp Ile Gln 1310 1315 1320 His Val Arg Lys Asp Asp Lys Asp Phe Asn Arg His Ala Tyr Asn 1325 1330 1335 Lys Asn Glu Tyr Ile Leu Asn Asp Ile Lys Phe Asn Pro Asn Ile 1340 1345 1350 Glu Gln Tyr Leu Arg Gln Leu Thr Gly Phe Ile Lys Leu Leu Thr 1355 1360 1365 Asp Met Lys Phe Thr Asn Phe Leu Phe Leu Arg Asn Leu Tyr Tyr 1370 1375 1380 Phe Val Lys Phe Tyr Ala Val Thr Gly Asp Leu Thr Tyr Ser Ile 1385 1390 1395 Asn Asn Ser Ser Ile Tyr Leu Cys Ser Arg Asn Tyr Leu Glu Phe 1400 1405 1410 Ile Leu Asn Ser Ile Ile Glu Phe Glu Asn Phe Lys Lys Phe Met 1415 1420 1425 Val Lys Ile Lys Glu Lys Phe Asp Ile Glu Lys Tyr Pro Ile Asp 1430 1435 1440 Pro Tyr Asn Phe Gln Thr Glu Cys Tyr Ser Ile Asp Ser Val Lys 1445 1450 1455 Thr Tyr Arg Phe Phe Leu Ser Asp Leu Asp Lys Leu Lys Ser Tyr 1460 1465 1470 Gln Asp Ile Glu Asn Leu Gln Asn Val Ser Ser Phe Tyr Lys Asp 1475 1480 1485 Tyr Leu Tyr Met Gly Leu Phe Tyr Glu Asn Leu Asp Val Arg Asn 1490 1495 1500 Leu Asn Val Tyr Tyr Asn Phe Asn Lys Lys Lys Asn Gln Asp His 1505 1510 1515 Lys Ala His Gly Ser Asn Val Phe Leu Asp Gly Gly Leu Ser Leu 1520 1525 1530 Ser Lys Asn Met Ser Phe Ser Asp Ile Asn Asp Leu Ser Glu Ser 1535 1540 1545 Ile Gln Asn Tyr Leu Tyr Leu Glu Lys Phe Leu Ala Asp Ser Asn 1550 1555 1560 Ile Pro Ser Ile Pro Tyr Gln Gly Phe Ser Val Arg Arg Val Asn 1565 1570 1575 Asp Gly Met His Val Thr Tyr Asn Asn Arg Ser Thr Thr Pro Pro 1580 1585 1590 Leu Tyr Lys Asp Asn Val Leu Asp Gln Phe Glu Leu Val Gly Lys 1595 1600 1605 Ser Leu Ile Ser Glu Tyr Phe Gln Lys Val Lys Cys Ser Phe Ile 1610 1615 1620 Gln Tyr Pro Phe Asn Leu Tyr Tyr Trp Leu Val Leu Asn Pro Gly 1625 1630 1635 Lys Pro Asn Ile Ser Leu Ser Ser Lys Thr Gly Leu Ile Lys Glu 1640 1645 1650 Asp Asp Leu Ile Pro Lys Thr Val Glu Glu Lys Leu Lys Glu Glu 1655 1660 1665 Glu Glu His Asp Ile Lys Ile Val Glu Asn Ile Leu Ser Glu Asp 1670 1675 1680 Ile Phe Asp Phe Lys Asp Lys Ser Asp Asp Asp Gln Ser Thr Thr 1685 1690 1695 Ala Asp Val Leu Ser Thr Asp Thr Asp Asp Ser Glu Thr Asp Ala 1700 1705 1710 Glu Lys Thr Ser Asn Asn Ser Asn Thr Leu His Lys Lys Glu Thr 1715 1720 1725 Pro Ala Met Lys Tyr Asn Met Asn Ile Ala Gln Asp Glu Ile Asn 1730 1735 1740 Arg Gln Asn Asp Val Ser Lys Asn Thr Thr Tyr Ala Glu Asn Asn 1745 1750 1755 Glu Tyr Thr Ser Glu Asn Ile Thr Lys Pro Ser Asp Gln Asn Thr 1760 1765 1770

Glu Asn Tyr Gly Thr Lys Met Pro Ser Pro Ser Phe Ile Gln Ile 1775 1780 1785 Asp Lys Val Asn Gln Gln Asp Gln Gln Asn Asp Ala His Ile Glu 1790 1795 1800 Ser Ser Lys Asn Thr Gln Glu Asn Leu Asn Tyr Asp Asp Asn Thr 1805 1810 1815 Asn Met Tyr Glu Glu Asn Lys Asn Asp Lys Lys Asn Lys Lys Leu 1820 1825 1830 Asn Asn Asn Thr Lys Glu Ile Ser Phe Leu Glu Arg Arg Glu Thr 1835 1840 1845 Asn Pro Ile Pro Phe Ser Ser Asn Met Ile Asn Glu Lys Asn Lys 1850 1855 1860 Glu Ala His Lys Glu His Tyr Gly Asn Phe Asn Arg Pro Tyr Asn 1865 1870 1875 Ile Tyr Val Pro Lys Lys Asp Ile Phe Arg Asn Phe His Leu Val 1880 1885 1890 Val Lys Val Val His Ala Leu Ile Ser Leu Asn Lys Phe Ser Met 1895 1900 1905 Val Ser Pro Asp Thr Ile Leu Lys Lys Gly Ile Glu Ser Met Lys 1910 1915 1920 Lys Lys His Ser Leu Lys Met Ile Lys Asn Ile Asn Pro Phe Ile 1925 1930 1935 His Lys Met Ile Leu Asp Met Asn Glu Thr Ile Gln Lys Leu Lys 1940 1945 1950 Ser Asp Ser Glu Lys Ala Tyr Gly Gly Pro Lys Ala Asp Ile Ile 1955 1960 1965 Ser Leu Tyr Lys Ile Val Glu Phe Gln Leu Phe Asn Gln Tyr Ile 1970 1975 1980 Ile Tyr Pro Pro Leu Lys Arg Leu Thr Lys Lys Glu Leu Lys Tyr 1985 1990 1995 Ile Leu Asp Val Val Asn Gln Gly Tyr Tyr Phe Tyr Leu Lys Asn 2000 2005 2010 Val Ile Thr Lys Leu Lys Arg Glu Asn Leu Gln Lys Ser Lys Val 2015 2020 2025 Val Lys Ile Phe Asn Asn Phe Gln Glu Phe Ser Asn Leu Leu Asp 2030 2035 2040 Asp Asp Gly Val Asp Lys Leu Tyr Asn Ile Phe Thr Glu Thr Phe 2045 2050 2055 Lys Cys Lys Asn Ile Lys Cys Phe Asp Leu Leu Phe Gln Gly Phe 2060 2065 2070 Leu Thr Glu Gln Tyr Asn Asn Ile Val Tyr Arg Tyr Thr His Asp 2075 2080 2085 His Asp Ala Met Asn Ser Tyr Asn Asp Asn Ile Phe Asn Asn Lys 2090 2095 2100 Glu Leu Leu Asn Lys Ile Phe Arg Arg Ala Tyr Asp Asn Tyr Phe 2105 2110 2115 Ile Thr Pro Val Lys Asn Asn Gln Pro Lys Lys Leu Ile Ser Leu 2120 2125 2130 Glu Lys Pro Ser Leu Glu Gln Pro Val Leu Thr Leu Glu Glu Phe 2135 2140 2145 Ala Tyr Gly Phe His Glu Phe Gly Asn Lys Ile Glu Lys Trp Arg 2150 2155 2160 Ser Met Leu Thr Leu Phe Asn Ile Arg His Ile Tyr Ile Asn Ile 2165 2170 2175 Cys His Met Leu Leu Ser Val Lys His Ser Ile Gln Arg Ser His 2180 2185 2190 Arg Val Phe Met His Lys Phe Gly Phe Phe Gly Ile Phe Arg Arg 2195 2200 2205 Lys Arg Glu Ile Tyr Ile Pro 2210 2215 351728DNAPlasmodium falciparum 35atgtttattg ctgttgtggg ttgtactcac ggagaattag atttaatata ctcgactctc 60gaaaagatag aagaagaaaa taaaataaag gttgaccttt taatttgttg tggtgatttt 120caaagtgtac gatataatgt tgataatgag tgtttgaatg ttcctgcgaa atataaaaaa 180gaacaaaatg atttcgtaga ttattttaca ggaaaaaaga aggcgaagat cttaactata 240tttgtaggag gaaaccatga ggccatgaat gttttgaaac agttgtatta tggtgggtgg 300gttgctccta atatatatta tttaggatac tctagtgttc ataatattaa taatttcaga 360atatgtagtt taagcggtat atataaaaaa tatagttttt ttaagaaata ttatgaatcc 420tatccttata cagatattac aaaggttagc gcatatcata taagaaaata tgaaatagaa 480aaattaaaat tattaaaaaa taacgttgat attgttgtaa cacatgattg gccaaataat 540attgaaaaac atggagatgt acatgactta ttaagaagaa aatatcattt tcaatctgac 600gtatataata atacattagg taatcctcat accgaaatat tattaaataa attaaaacct 660tatttctggt ttgcatcaca tttacatgta aaatattcgg cattgtatat ccacaatgat 720cagaaacagt atactagatt tctttctcta gataaggctc aagaatataa acattttata 780caaattttaa atattgtaaa gaaaaaggat tcttctatac atttaaattt tgatcatgtt 840cctaaggtgt tgttaccaga accaggtagc aaaatggaca ttcaaaatga tgcacagcct 900aatcatgatt tggagaattg tcctaataca aaaacaaata catgcaacaa taatgatcat 960cataatgatg attctattaa tttagattat gatcatgaaa aggcattata cgaactagac 1020agaaatatgc aactggatca ggaaaaaaat gacgaaaaaa atgttgacaa aagtgcagat 1080aaaaatgtgt gcaataaaga catatcctta gaagataaaa atcaacataa taataacaat 1140aataataatg atgatgatga tgatggtgta gatatacaag ctgatacatc aacaaatgtt 1200gctgatcaaa ataataactc tgttccaaca aatttaaaag aaaatgagga agaatcttta 1260aatgatcaaa atgaaaataa agatgaagaa actagccaag atgaaaatat aacagatgaa 1320aaaaaaaaaa aaaaatttta tttatgttat gatatagaat ggctagctat agtaaaagca 1380aatcatcatc tcatttctgc ctcatgtgat aaggattata atttagaaac actaacatat 1440ccaacaaaag aagatttcga ttttgtagag aataaactta aagagttgga taataaaata 1500acaataaaag gtaaagatta ttattgtgta aatggttata atacgccaaa ttataaaaac 1560ctccaagaac aaagacagtt atttttaaaa cgtttcgaat tagaggagtt aagtatatac 1620acagagtcag agttgaattt ttttgctgaa gaaatgaaaa cattggaaaa aatgaatact 1680gatattcata atgaggagga taagaatgaa tgtactatag aagcatag 172836575PRTPlasmodium falciparum 36Met Phe Ile Ala Val Val Gly Cys Thr His Gly Glu Leu Asp Leu Ile 1 5 10 15 Tyr Ser Thr Leu Glu Lys Ile Glu Glu Glu Asn Lys Ile Lys Val Asp 20 25 30 Leu Leu Ile Cys Cys Gly Asp Phe Gln Ser Val Arg Tyr Asn Val Asp 35 40 45 Asn Glu Cys Leu Asn Val Pro Ala Lys Tyr Lys Lys Glu Gln Asn Asp 50 55 60 Phe Val Asp Tyr Phe Thr Gly Lys Lys Lys Ala Lys Ile Leu Thr Ile 65 70 75 80 Phe Val Gly Gly Asn His Glu Ala Met Asn Val Leu Lys Gln Leu Tyr 85 90 95 Tyr Gly Gly Trp Val Ala Pro Asn Ile Tyr Tyr Leu Gly Tyr Ser Ser 100 105 110 Val His Asn Ile Asn Asn Phe Arg Ile Cys Ser Leu Ser Gly Ile Tyr 115 120 125 Lys Lys Tyr Ser Phe Phe Lys Lys Tyr Tyr Glu Ser Tyr Pro Tyr Thr 130 135 140 Asp Ile Thr Lys Val Ser Ala Tyr His Ile Arg Lys Tyr Glu Ile Glu 145 150 155 160 Lys Leu Lys Leu Leu Lys Asn Asn Val Asp Ile Val Val Thr His Asp 165 170 175 Trp Pro Asn Asn Ile Glu Lys His Gly Asp Val His Asp Leu Leu Arg 180 185 190 Arg Lys Tyr His Phe Gln Ser Asp Val Tyr Asn Asn Thr Leu Gly Asn 195 200 205 Pro His Thr Glu Ile Leu Leu Asn Lys Leu Lys Pro Tyr Phe Trp Phe 210 215 220 Ala Ser His Leu His Val Lys Tyr Ser Ala Leu Tyr Ile His Asn Asp 225 230 235 240 Gln Lys Gln Tyr Thr Arg Phe Leu Ser Leu Asp Lys Ala Gln Glu Tyr 245 250 255 Lys His Phe Ile Gln Ile Leu Asn Ile Val Lys Lys Lys Asp Ser Ser 260 265 270 Ile His Leu Asn Phe Asp His Val Pro Lys Val Leu Leu Pro Glu Pro 275 280 285 Gly Ser Lys Met Asp Ile Gln Asn Asp Ala Gln Pro Asn His Asp Leu 290 295 300 Glu Asn Cys Pro Asn Thr Lys Thr Asn Thr Cys Asn Asn Asn Asp His 305 310 315 320 His Asn Asp Asp Ser Ile Asn Leu Asp Tyr Asp His Glu Lys Ala Leu 325 330 335 Tyr Glu Leu Asp Arg Asn Met Gln Leu Asp Gln Glu Lys Asn Asp Glu 340 345 350 Lys Asn Val Asp Lys Ser Ala Asp Lys Asn Val Cys Asn Lys Asp Ile 355 360 365 Ser Leu Glu Asp Lys Asn Gln His Asn Asn Asn Asn Asn Asn Asn Asp 370 375 380 Asp Asp Asp Asp Gly Val Asp Ile Gln Ala Asp Thr Ser Thr Asn Val 385 390 395 400 Ala Asp Gln Asn Asn Asn Ser Val Pro Thr Asn Leu Lys Glu Asn Glu 405 410 415 Glu Glu Ser Leu Asn Asp Gln Asn Glu Asn Lys Asp Glu Glu Thr Ser 420 425 430 Gln Asp Glu Asn Ile Thr Asp Glu Lys Lys Lys Lys Lys Phe Tyr Leu 435 440 445 Cys Tyr Asp Ile Glu Trp Leu Ala Ile Val Lys Ala Asn His His Leu 450 455 460 Ile Ser Ala Ser Cys Asp Lys Asp Tyr Asn Leu Glu Thr Leu Thr Tyr 465 470 475 480 Pro Thr Lys Glu Asp Phe Asp Phe Val Glu Asn Lys Leu Lys Glu Leu 485 490 495 Asp Asn Lys Ile Thr Ile Lys Gly Lys Asp Tyr Tyr Cys Val Asn Gly 500 505 510 Tyr Asn Thr Pro Asn Tyr Lys Asn Leu Gln Glu Gln Arg Gln Leu Phe 515 520 525 Leu Lys Arg Phe Glu Leu Glu Glu Leu Ser Ile Tyr Thr Glu Ser Glu 530 535 540 Leu Asn Phe Phe Ala Glu Glu Met Lys Thr Leu Glu Lys Met Asn Thr 545 550 555 560 Asp Ile His Asn Glu Glu Asp Lys Asn Glu Cys Thr Ile Glu Ala 565 570 575 372739DNAPlasmodium falciparum 37atgaatttcc taatattagt tgtattaatt aagatggttg tatgtaaaat tattaattat 60gttagaagtg attataattt tatgctgtca tttttaagga cgagaaaaat atttagtcat 120aaaaagattt atttcaattt taaagtttct aagaatatta caaagaattc atcatctaat 180agttatagtg atatattaaa atgtttctct tttccaagaa aaggaactaa cgataagacg 240aaggaaaatg aaacatttga aaaaggagaa gtaaaagaag aagaagaaga aaaagataaa 300aacgtcaaaa ggaaaattat taatgaaagt agtaaaaata aaagagctaa agttaagaat 360gaaaatgatg ttataaaaaa agggtcgtta tttaattgtg ttgtaagaga agatgaaaaa 420gtgaatgatt tgagctcacc taaatttaat cctgtacatt ttaatataaa tgatttatat 480ttatctgaaa aagataaaga aaaacataaa tttaaagact ccttattatt taccttttta 540acaaatgcat ttaatcaaat agaagaatta aaaggtagtg gtacaggaag taaaaaaaat 600gtatctatta tattatcgaa tatatttagg gttttaatat attatagccc taatgactta 660ataccagctg tatatataac cttaaataaa gtagctcccg attatttaaa tgtcgaagct 720ggtgtaggtg aagccttgat attaaaaacg atgtctgaag catatagtag aacggaaagt 780agtataaaga aggatttaca acaaattgaa gatttaggaa taatagcgga gaattgttct 840tgtaaaatga gaacaatatt tccgttacct cgcttaacta tacaatctgt ttttcatgag 900ctaaaaagta tacctaacct tataggatca aattcacaac agaagaaaag ggaagtcata 960aagaaattgt tagtaagtgc taaaacatct gaagctaaat atattgtaag atttttacaa 1020cagcgtttaa ggataggtgt aaattctgca acggtcgtac aagctttgtc atatgccttt 1080atattaacaa gacctagtat acctgatgat attataaaaa gagggaaaga aattaatgag 1140aatttgatta atggtaaatt ggatggtact aataatttaa ttaataaatc taataacaag 1200acggaagagt ggctaaccca atcaggtgat aggaattgta taaaggaaga aaatttaaat 1260gatgaattat tatcaaagga tgatataaat aaatgtgaaa acatggattc attaattaat 1320gagatacgta ttcgaaatga aaaaataaac aaaccgaata ttttttataa ccttattgaa 1380aaggtgggtg acacacgatt attaccatta ttttttaaag aattaaaaaa tatatattgt 1440gaagaaaata atgatattga tatatttgaa tgtatggaaa aatcagttaa gagtgcttta 1500tgtgaattac ccaatatcga aataataata caaaatttat taaatggtga tgatatgaat 1560accttaagta aaaaatgtac tgttaaaaca ggtattcccg ttcaacctat gttagcaaaa 1620ccaactaagg gtgttcaaga agtgttggat agatttaata atgttacatt tacttgtgaa 1680tataaatatg atggtgaacg agcacaaata cattatatag ataaagataa tataaaaata 1740ttcagtcgaa atttagaaac gatgactgaa aaatatccag atgttataca aataataaag 1800gatcaaatag gagagaatgt aaaggaatgt attatagata gtgaagttgt tgcttatgat 1860atagttaata ataaaatatt accattccaa gtcttaacta ctagaaaaag aaaagatgta 1920gatatagaaa atattaaagt gaaagtatgt ctttttccat ttgatttaat atgttgtaat 1980ggtataccag taattaaaga acctttagct gttagaagga aattattata ttctttatta 2040aagtcaaaag atggggtgtt atcatatgct acccattctg aaatgaataa tattgaagat 2100attgatatgt tcttacaaga tgctatcgaa aataattgtg aaggtttaat ggttaagact 2160ttagtagaaa atgcatccta tgaaccttct aggagatcat taaattggtt aaaggtcaag 2220aaagattatg ttgaaggtct ttcagattcg gttgatttag ttccgatagc tggttattat 2280gggaaaggaa aaagaagtgg agtatatgga gcttttgttt tagcagctta taattctgaa 2340acggaaaatt ttcaaaccgt ttgtaaggca ggtacaggtt ttagtgatga gatattaagt 2400acattatatg aaacattaag tgaaaaaatt ataccaaata aaaaatcgta ttacgaagta 2460tctgacaaat taaatcctga tgtgtggttt gatgctcact atgtatggga ggttaaggca 2520gcagatttat ctttgtcacc tgtacataca gcagctattg gtatatatgc agatgataaa 2580ggtattggtt taaggtttcc tcgattttta cgtttaagag aagataaaaa tgcagaacaa 2640gcaaccacta cacaacaaat tgtagatttt tatgaggcac aatttagtag taataaaaat 2700aaaaatattg attataatga tgatactgaa agtgaataa 273938912PRTPlasmodium falciparum 38Met Asn Phe Leu Ile Leu Val Val Leu Ile Lys Met Val Val Cys Lys 1 5 10 15 Ile Ile Asn Tyr Val Arg Ser Asp Tyr Asn Phe Met Leu Ser Phe Leu 20 25 30 Arg Thr Arg Lys Ile Phe Ser His Lys Lys Ile Tyr Phe Asn Phe Lys 35 40 45 Val Ser Lys Asn Ile Thr Lys Asn Ser Ser Ser Asn Ser Tyr Ser Asp 50 55 60 Ile Leu Lys Cys Phe Ser Phe Pro Arg Lys Gly Thr Asn Asp Lys Thr 65 70 75 80 Lys Glu Asn Glu Thr Phe Glu Lys Gly Glu Val Lys Glu Glu Glu Glu 85 90 95 Glu Lys Asp Lys Asn Val Lys Arg Lys Ile Ile Asn Glu Ser Ser Lys 100 105 110 Asn Lys Arg Ala Lys Val Lys Asn Glu Asn Asp Val Ile Lys Lys Gly 115 120 125 Ser Leu Phe Asn Cys Val Val Arg Glu Asp Glu Lys Val Asn Asp Leu 130 135 140 Ser Ser Pro Lys Phe Asn Pro Val His Phe Asn Ile Asn Asp Leu Tyr 145 150 155 160 Leu Ser Glu Lys Asp Lys Glu Lys His Lys Phe Lys Asp Ser Leu Leu 165 170 175 Phe Thr Phe Leu Thr Asn Ala Phe Asn Gln Ile Glu Glu Leu Lys Gly 180 185 190 Ser Gly Thr Gly Ser Lys Lys Asn Val Ser Ile Ile Leu Ser Asn Ile 195 200 205 Phe Arg Val Leu Ile Tyr Tyr Ser Pro Asn Asp Leu Ile Pro Ala Val 210 215 220 Tyr Ile Thr Leu Asn Lys Val Ala Pro Asp Tyr Leu Asn Val Glu Ala 225 230 235 240 Gly Val Gly Glu Ala Leu Ile Leu Lys Thr Met Ser Glu Ala Tyr Ser 245 250 255 Arg Thr Glu Ser Ser Ile Lys Lys Asp Leu Gln Gln Ile Glu Asp Leu 260 265 270 Gly Ile Ile Ala Glu Asn Cys Ser Cys Lys Met Arg Thr Ile Phe Pro 275 280 285 Leu Pro Arg Leu Thr Ile Gln Ser Val Phe His Glu Leu Lys Ser Ile 290 295 300 Pro Asn Leu Ile Gly Ser Asn Ser Gln Gln Lys Lys Arg Glu Val Ile 305 310 315 320 Lys Lys Leu Leu Val Ser Ala Lys Thr Ser Glu Ala Lys Tyr Ile Val 325 330 335 Arg Phe Leu Gln Gln Arg Leu Arg Ile Gly Val Asn Ser Ala Thr Val 340 345 350 Val Gln Ala Leu Ser Tyr Ala Phe Ile Leu Thr Arg Pro Ser Ile Pro 355 360 365 Asp Asp Ile Ile Lys Arg Gly Lys Glu Ile Asn Glu Asn Leu Ile Asn 370 375 380 Gly Lys Leu Asp Gly Thr Asn Asn Leu Ile Asn Lys Ser Asn Asn Lys 385 390 395 400 Thr Glu Glu Trp Leu Thr Gln Ser Gly Asp Arg Asn Cys Ile Lys Glu 405 410 415 Glu Asn Leu Asn Asp Glu Leu Leu Ser Lys Asp Asp Ile Asn Lys Cys 420 425 430 Glu Asn Met Asp Ser Leu Ile Asn Glu Ile Arg Ile Arg Asn Glu Lys 435 440 445 Ile Asn Lys Pro Asn Ile Phe Tyr Asn Leu Ile Glu Lys Val Gly Asp 450 455 460 Thr Arg Leu Leu Pro Leu Phe Phe Lys Glu Leu Lys Asn Ile Tyr Cys 465 470 475 480 Glu Glu Asn Asn Asp Ile Asp Ile Phe Glu Cys Met Glu Lys Ser Val 485 490 495 Lys Ser Ala Leu Cys Glu Leu Pro Asn Ile Glu Ile Ile Ile Gln Asn 500 505 510 Leu Leu Asn Gly Asp Asp Met Asn Thr Leu Ser Lys Lys Cys Thr Val 515 520 525 Lys Thr Gly Ile Pro Val Gln Pro Met Leu Ala Lys Pro Thr Lys Gly 530 535 540 Val Gln Glu Val Leu Asp Arg Phe Asn Asn Val Thr Phe Thr Cys Glu 545 550 555

560 Tyr Lys Tyr Asp Gly Glu Arg Ala Gln Ile His Tyr Ile Asp Lys Asp 565 570 575 Asn Ile Lys Ile Phe Ser Arg Asn Leu Glu Thr Met Thr Glu Lys Tyr 580 585 590 Pro Asp Val Ile Gln Ile Ile Lys Asp Gln Ile Gly Glu Asn Val Lys 595 600 605 Glu Cys Ile Ile Asp Ser Glu Val Val Ala Tyr Asp Ile Val Asn Asn 610 615 620 Lys Ile Leu Pro Phe Gln Val Leu Thr Thr Arg Lys Arg Lys Asp Val 625 630 635 640 Asp Ile Glu Asn Ile Lys Val Lys Val Cys Leu Phe Pro Phe Asp Leu 645 650 655 Ile Cys Cys Asn Gly Ile Pro Val Ile Lys Glu Pro Leu Ala Val Arg 660 665 670 Arg Lys Leu Leu Tyr Ser Leu Leu Lys Ser Lys Asp Gly Val Leu Ser 675 680 685 Tyr Ala Thr His Ser Glu Met Asn Asn Ile Glu Asp Ile Asp Met Phe 690 695 700 Leu Gln Asp Ala Ile Glu Asn Asn Cys Glu Gly Leu Met Val Lys Thr 705 710 715 720 Leu Val Glu Asn Ala Ser Tyr Glu Pro Ser Arg Arg Ser Leu Asn Trp 725 730 735 Leu Lys Val Lys Lys Asp Tyr Val Glu Gly Leu Ser Asp Ser Val Asp 740 745 750 Leu Val Pro Ile Ala Gly Tyr Tyr Gly Lys Gly Lys Arg Ser Gly Val 755 760 765 Tyr Gly Ala Phe Val Leu Ala Ala Tyr Asn Ser Glu Thr Glu Asn Phe 770 775 780 Gln Thr Val Cys Lys Ala Gly Thr Gly Phe Ser Asp Glu Ile Leu Ser 785 790 795 800 Thr Leu Tyr Glu Thr Leu Ser Glu Lys Ile Ile Pro Asn Lys Lys Ser 805 810 815 Tyr Tyr Glu Val Ser Asp Lys Leu Asn Pro Asp Val Trp Phe Asp Ala 820 825 830 His Tyr Val Trp Glu Val Lys Ala Ala Asp Leu Ser Leu Ser Pro Val 835 840 845 His Thr Ala Ala Ile Gly Ile Tyr Ala Asp Asp Lys Gly Ile Gly Leu 850 855 860 Arg Phe Pro Arg Phe Leu Arg Leu Arg Glu Asp Lys Asn Ala Glu Gln 865 870 875 880 Ala Thr Thr Thr Gln Gln Ile Val Asp Phe Tyr Glu Ala Gln Phe Ser 885 890 895 Ser Asn Lys Asn Lys Asn Ile Asp Tyr Asn Asp Asp Thr Glu Ser Glu 900 905 910

Claims

1. An immunogenic composition comprising a nucleic acid sequence expressed by a suitable expression system, wherein said nucleic acid sequence expresses an isolated protein expressed by Plasmodium falciparum sporozoites encoded by one or more of the locus selected from the group consisting of PF10925w, PF14_0051, PFL2140c, PFE1085w, and PF13_0222, wherein the isolated protein has the amino acid sequence selected from the group consisting of SEQ ID No. 2, 6, 24, 28, and 36, and wherein said isolated protein is expressed from a nucleotide sequence, inserted into a suitable expression vector capable of protein expression in a mammal, wherein the nucleotide sequence comprises the nucleotide sequence selected from the group consisting of SEQ ID No. 1, 5, 23, 27, and 35, wherein said suitable expression vector is a DNA plasmid or replicating or nonreplicating viral vector.

2. The immunogenic composition of claim 1, wherein the composition also comprises a nucleic acid sequence expressed by a suitable expression system, wherein said nucleic acid sequence expresses isolated proteins expressed by Plasmodium falciparum sporozoites encoded by one or more of the loci selected from the group consisting of PFB0285c, PFL1620w, PF10_0211, PFB0150c, PF11_0344, PFE0060w, PF08_0034, PF08_0054, PFC0210c, PF11_0404, PF13_0201, PFL2505c, PF13_0222, and MAL13P1.22, wherein the isolated proteins have the amino acid sequences selected from the group consisting of, with the amino acid sequences of SEQ ID No. 4, SEQ ID No. 10, SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, SEQ ID No. 20, SEQ ID No. 22, SEQ ID 26, SEQ ID No. 30, SEQ ID No. 32, SEQ ID No. 34, and SEQ ID No. 38, wherein the one or more isolated proteins are encoded by one or more the nucleic acid sequences of SEQ ID No. 3, SEQ ID No. 9, SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, SEQ ID No. 19, SEQ ID No. 21, SEQ ID 25, SEQ ID No. 29, SEQ ID No. 31, SEQ ID No. 33, and SEQ ID No. 37 and inserted into and expressed from a suitable expression system, wherein said suitable expression vector is a DNA plasmid or replicating or nonreplicating viral vector.

3. The immunogenic composition of claim 1, wherein said viral vector is selected from the group consisting of DNA plasmid, alphavirus replicon, adenovirus, poxvirus, adeno-associated virus, cytomegalovirus, canine distemper virus, yellow fever virus and retrovirus.

4. The immunogenic composition of claim 3, wherein the alphavirus replicon preparation is selected from the group consisting of RNA replicons, DNA replicons and alphavirus replicon particles.

5. The immunogenic composition of claim 4, wherein the alphavirus is selected from the group consisting of Venzuelean Equine Encephalitis Virus, Semliki Forest Virus and Sindbis Virus.

6. The immunogenic composition of claim 3, wherein the poxvirus is selected from the group consisting of cowpox, canarypox, vaccinia, modified vaccinia Ankara, or fowlpox.

7. A method of inducing an immune response against malaria in a mammal, which method comprises administering to a mammal a composition comprising one or more isolated nucleic acid molecules, inserted into a suitable expression vector, encoding isolated malaria polypeptides, wherein said isolated nucleic acid molecules are as in claims 1 or 2.

8. The method of claim 7, where the suitable expression system is a DNA plasmid or replicating or nonreplicating viral vector.

9. The method of claim 7, wherein said method further comprises one or more priming and one or more boosting immunizations, wherein said priming immunizations comprise one or more malaria polypeptides, as in claim 1 or 2, inserted into a suitable expression vector, and wherein said boosting immunizations comprise a malaria polypeptide, as in claim 1 or 2, inserted into suitable expression vector.

10. The method of claim 7, wherein said suitable expression vector is selected from the group consisting of DNA plasmid alphavirus replicon, adenovirus, poxvirus, adenoassociated virus, cytomegalovirus, canine distemper virus, yellow fever virus and retrovirus.

11. The method of claim 9, wherein said priming immunization vector is an alphavirus vector and said boosting immunization vector is nonalphavirus vector.

12. The method of claim 9, wherein said priming immunization comprises an expression vector that is a DNA plasmid or an adenovirus and the boosting immunization is selected from the group consisting of adenovirus, adenovirus heterologous to the priming adenovirus, poxvirus and polypeptide, wherein said polypeptides have amino acid sequences as in claims 1 or 2.

13. The method of claim 10, wherein the alphavirus replicon is selected from the group consisting of RNA replicons, DNA replicons and alphavirus replicon particles.

14. The method of claim 11, wherein the non-alphavirus viral is selected from the group consisting of poxvirus, adenovirus, adeno-associated virus and retrovirus.

15. The method of claim 13, wherein the alphavirus is selected from the group consisting of Venzuelean Equine Encephalitis Virus, Semliki Forest Virus and Sindbis Virus.

16. The method of claim 14, wherein the poxvirus is selected from the group consisting of cowpox, canarypox, vaccinia, modified vaccinia Ankara, or fowlpox.

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