THIOESTERASES AND CELLS FOR PRODUCTION OF TAILORED OILS

Abstract

The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of U.S. application Ser. No. 13/837,996, filed on Mar. 15, 2013, issued as U.S. Pat. No. 9,290,749 on Mar. 22, 2016, which is hereby incorporated herein by reference in its entirety for all purposes.

SEQUENCE LISTING

[0002] The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jul. 3, 2013, is named SOLAP019US_SL.txt and is 318,250 bytes in size.

BACKGROUND

[0003] Certain organisms including plants and some microalgae use a type II fatty acid biosynthetic pathway, characterized by the use of discrete, monofunctional enzymes for fatty acid synthesis. In contrast, mammals and fungi use a single, large, multifunctional protein.

[0004] Type II fatty acid biosynthesis typically involves extension of a growing acyl-ACP (acyl-carrier protein) chain by two carbon units followed by cleavage by an acyl-ACP thioesterase. In plants, two main classes of acyl-ACP thioesterases have been identified: (i) those encoded by genes of the FatA class, which tend to hydrolyze oleoyl-ACP into oleate (an 18:1 fatty acid) and ACP, and (ii) those encoded by genes of the FatB class, which liberate C8-C16 fatty acids from corresponding acyl-ACP molecules.

[0005] Different FatB genes from various plants have specificities for different acyl chain lengths. As a result, different gene products will produce different fatty acid profiles in plant seeds. See, U.S. Pat. Nos. 5,850,022; 5,723,761; 5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482; 5,298,421; 5,667,997; and U.S. Pat. Nos. 5,344,771; 5,304,481. Recently, FatB genes have been cloned into oleaginous microalgae to produce triglycerides with altered fatty acid profiles. See, WO2010/063032, WO2011,150411, and WO2012/106560.

SUMMARY

[0006] In an embodiment of the invention, there is a nucleic acid having at least 80% sequence identity to any of SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76 or any equivalent sequences by virtue of the degeneracy of the genetic code.

[0007] In another embodiment of the invention, there is a nucleic acid sequence encoding a protein having at least 80% sequence identity to any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, or 77, or a fragment thereof having acyl-ACP thioesterase activity. The protein can have acyl-ACP thioesterase activity operable to alter the fatty acid profile of an oil produced by a recombinant cell comprising that sequence.

[0008] In a further embodiment of the invention there is a method of producing a recombinant host cell that produces an altered fatty acid profile, the method comprising transforming the cell with any of the nucleic acids mentioned above. The host cell can be a plant cell, a microbial cell, or a microalgal cell. Another embodiment of the invention includes a host cell produced by this method.

[0009] In an embodiment, there is a method for producing an oil or oil-derived product, the method comprising cultivating the host cell and extracting the oil, optionally wherein the cultivation is heterotrophic growth on sugar. Optionally, a fatty acid, fuel, chemical, or other oil-derived product can be produced from the oil. Optionally, the oil can have a fatty acid profile comprising at least 20% C8, C10, C12, C14 or C16 fatty acids. Optionally, the oil is produced by a microalgae and can lack C24-alpha sterols.

DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS OF THE INVENTION

Definitions

[0010] As used with respect to nucleic acids, the term "isolated" refers to a nucleic acid that is free of at least one other component that is typically present with the naturally occurring nucleic acid. Thus, a naturally occurring nucleic acid is isolated if it has been purified away from at least one other component that occurs naturally with the nucleic acid.

[0011] A "natural oil" or "natural fat" shall mean a predominantly triglyceride oil obtained from an organism, where the oil has not undergone blending with another natural or synthetic oil, or fractionation so as to substantially alter the fatty acid profile of the triglyceride. In connection with an oil comprising triglycerides of a particular regiospecificity, the natural oil or natural fat has not been subjected to interesterification or other synthetic process to obtain that regiospecific triglyceride profile, rather the regiospecificity is produced naturally, by a cell or population of cells. In connection with a natural oil or natural fat, and as used generally throughout the present disclosure, the terms oil and fat are used interchangeably, except where otherwise noted. Thus, an "oil" or a "fat" can be liquid, solid, or partially solid at room temperature, depending on the makeup of the substance and other conditions. Here, the term "fractionation" means removing material from the oil in a way that changes its fatty acid profile relative to the profile produced by the organism, however accomplished. The terms "natural oil" and "natural fat" encompass such oils obtained from an organism, where the oil has undergone minimal processing, including refining, bleaching and/or degumming, that does not substantially change its triglyceride profile. A natural oil can also be a "noninteresterified natural oil", which means that the natural oil has not undergone a process in which fatty acids have been redistributed in their acyl linkages to glycerol and remain essentially in the same configuration as when recovered from the organism.

[0012] "Exogenous gene" shall mean a nucleic acid that codes for the expression of an RNA and/or protein that has been introduced into a cell (e.g. by transformation/transfection), and is also referred to as a "transgene". A cell comprising an exogenous gene may be referred to as a recombinant cell, into which additional exogenous gene(s) may be introduced. The exogenous gene may be from a different species (and so heterologous), or from the same species (and so homologous), relative to the cell being transformed. Thus, an exogenous gene can include a homologous gene that occupies a different location in the genome of the cell or is under different control, relative to the endogenous copy of the gene. An exogenous gene may be present in more than one copy in the cell. An exogenous gene may be maintained in a cell as an insertion into the genome (nuclear or plastid) or as an episomal molecule.

[0013] "Fatty acids" shall mean free fatty acids, fatty acid salts, or fatty acyl moieties in a glycerolipid. It will be understood that fatty acyl groups of glycerolipids can be described in terms of the carboxylic acid or anion of a carboxylic acid that is produced when the triglyceride is hydrolyzed or saponified.

[0014] "Microalgae" are microbial organisms that contain a chloroplast or other plastid, and optionally that are capable of performing photosynthesis, or a prokaryotic microbial organism capable of performing photosynthesis. Microalgae include obligate photoautotrophs, which cannot metabolize a fixed carbon source as energy, as well as heterotrophs, which can live solely off of a fixed carbon source. Microalgae include unicellular organisms that separate from sister cells shortly after cell division, such as Chlamydomonas, as well as microbes such as, for example, Volvox, which is a simple multicellular photosynthetic microbe of two distinct cell types. Microalgae include cells such as Chlorella, Dunaliella, and Prototheca. Microalgae also include other microbial photosynthetic organisms that exhibit cell-cell adhesion, such as Agmenellum, Anabaena, and Pyrobotrys. Microalgae also include obligate heterotrophic microorganisms that have lost the ability to perform photosynthesis, such as certain dinoflagellate algae species and species of the genus Prototheca.

[0015] An "oleaginous" cell is a cell capable of producing at least 20% lipid by dry cell weight, naturally or through recombinant or classical strain improvement. An "oleaginous microbe" or "oleaginous microorganism" is a microbe, including a microalga that is oleaginous.

[0016] In connection with a natural oil, a "profile" is the distribution of particular species or triglycerides or fatty acyl groups within the oil. A "fatty acid profile" is the distribution of fatty acyl groups in the triglycerides of the oil without reference to attachment to a glycerol backbone. Fatty acid profiles are typically determined by conversion to a fatty acid methyl ester (FAME), followed by gas chromatography (GC) analysis with flame ionization detection (FID). The fatty acid profile can be expressed as one or more percent of a fatty acid in the total fatty acid signal determined from the area under the curve for that fatty acid. FAME-GC-FID measurement approximate weight percentages of the fatty acids.

[0017] "Recombinant" is a cell, nucleic acid, protein or vector that has been modified due to the introduction of an exogenous nucleic acid or the alteration of a native nucleic acid. Thus, e.g., recombinant cells can express genes that are not found within the native (non-recombinant) form of the cell or express native genes differently than those genes are expressed by a non-recombinant cell. Recombinant cells can, without limitation, include recombinant nucleic acids that encode a gene product or suppression elements such as mutations, knockouts, antisense, interfering RNA (RNAi) or dsRNA that reduce the levels of active gene product in a cell. A "recombinant nucleic acid" is a nucleic acid originally formed in vitro, in general, by the manipulation of nucleic acid, e.g., using polymerases, ligases, exonucleases, and endonucleases, using chemical synthesis, or otherwise is in a form not normally found in nature. Recombinant nucleic acids may be produced, for example, to place two or more nucleic acids in operable linkage. Thus, an isolated nucleic acid or an expression vector formed in vitro by ligating DNA molecules that are not normally joined in nature, are both considered recombinant for the purposes of this invention. Once a recombinant nucleic acid is made and introduced into a host cell or organism, it may replicate using the in vivo cellular machinery of the host cell; however, such nucleic acids, once produced recombinantly, although subsequently replicated intracellularly, are still considered recombinant for purposes of this invention. Similarly, a "recombinant protein" is a protein made using recombinant techniques, i.e., through the expression of a recombinant nucleic acid.

Thioesterase Sequences

[0018] Additional FatB genes encoding thioesterases with varying substrate preferences have been identified from plant seeds. These genes or functional subsequences thereof can be used to engineer organisms to produce fatty acids having a chain-length distribution (fatty acid profile) that is altered from the wild type organism. Specifically, recombinant cells express one or more of the exogenous FatB genes. The fatty acids can be further converted to triglycerides, fatty aldehydes, fatty alcohols and other oleochemicals either synthetically or biosynthetically. In specific embodiments, triglycerides are produced by a host cell expressing the novel FatB gene. A triglyceride-containing natural oil can be recovered from the host cell. The natural oil can be refined, degummed, bleached and/or deodorized. The oil, in its natural or processed form, can be used for foods, chemicals, fuels, cosmetics, plastics, and other uses.

[0019] The genes can be used in a variety of genetic constructs including plasmids or other vectors for expression or recombination in a host cell. The genes can be codon optimized for expression in a target host cell. The proteins produced by the genes can be used in vivo or in purified form.

[0020] The gene sequences disclosed can also be used to prepare antisense, or inhibitory RNA (e.g., RNAi or hairpin RNA) to inhibit complementary genes in a plant or other organism.

[0021] FatB genes found to be useful in producing desired fatty acid profiles in a cell are summarized below in Table 1. Nucleic acids or proteins having the sequence of SEQ ID NOS: 1-78 can be used to alter the fatty acid profile of a recombinant cell. Variant nucleic acids can also be used; e.g, variants having at least 80, 85, 90, 95, 96, 97, 98, or 99% sequence identity to SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, or 78. Codon optimization of the genes for a variety of host organisms is contemplated, as is the use of gene fragments. Preferred codons for Prototheca strains and for Chlorella protothecoides are shown below in Tables 2 and 3, respectively. In some embodiments, the first and/or second most preferred Prototheca codons are employed for codon optimization.

[0022] In embodiments of the invention, there is protein or a nucleic acid encoding a protein having any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, or 77. In an embodiment, there is protein or a nucleic acid encoding a protein having at least 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100% sequence identity with any of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, or 77. In certain embodiments, the invention encompasses a fragment any of the above-described proteins or nucleic acids (including fragments of protein or nucleic acid variants), wherein the protein fragment has acyl-ACP thioesterase activity or the nucleic acid fragment encodes such a protein fragment. In other embodiments, the fragment includes a domain of an acyl-ACP thioesterase that mediates a particular function, e.g., a specificity-determining domain. Illustrative fragments can be produced by C-terminal and/or N-terminal truncations and include at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of the full-length sequences disclosed herein.

[0023] The term "percent sequence identity," in the context of two or more amino acid or nucleic acid sequences, refers to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same, when compared and aligned for maximum correspondence, as measured using a sequence comparison algorithm or by visual inspection. For sequence comparison to determine percent nucleotide or amino acid identity, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters. Optimal alignment of sequences for comparison can be conducted using the NCBI BLAST software (ncbi.nlm.nih.gov/BLAST/) set to default parameters. For example, to compare two nucleic acid sequences, one may use blastn with the "BLAST 2 Sequences" tool Version 2.0.12 (Apr. 21, 2000) set at the following default parameters: Matrix: BLOSUM62; Reward for match: 1; Penalty for mismatch: -2; Open Gap: 5 and Extension Gap: 2 penalties; Gap x drop-off: 50; Expect: 10; Word Size: 11; Filter: on. For a pairwise comparison of two amino acid sequences, one may use the "BLAST 2 Sequences" tool Version 2.0.12 (Apr. 21, 2000) with blastp set, for example, at the following default parameters: Matrix: BLOSUM62; Open Gap: 11 and Extension Gap: 1 penalties; Gap x drop-off 50; Expect: 10; Word Size: 3; Filter: on.

[0024] In certain embodiments, percent sequence identity for variants of the nucleic acids or proteins discussed above can be calculated by using the full-length nucleic acid sequence (e.g., one of SEQ ID NOS: 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 47, 48, 50, 51, 53, 54, 56, 57, 59, 60, 62, 63, 65, 66, 68, 69, 71, 72, 74, 76, or 78) or full-length amino acid sequence (e.g., one of SEQ ID NOS: 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 75, or 77) as the reference sequence and comparing the full-length test sequence to this reference sequence. In some embodiments relating to fragments, percent sequence identity for variants of nucleic acid or protein fragments can be calculated over the entire length of the fragment.

[0025] The nucleic acids can be in isolated form, or part of a vector or other construct, chromosome or host cell. It has been found that is many cases the full length gene (and protein) is not needed; for example, deletion of some or all of the N-terminal hydrophobic domain (typically an 18 amino acid domain starting with LPDW (SEQ ID NO: 115)) yields a still-functional gene. In addition, fusions of the specificity determining regions of the genes in Table 1 with catalytic domains of other acyl-ACP thioesterases can yield functional genes. Thus, in certain embodiments, the invention encompasses functional fragments (e.g., specificity determining regions) of the disclosed nucleic acid or amino acids fused to heterologous acyl-ACP thioesterase nucleic acid or amino acid sequences, respectively.

TABLE-US-00001 TABLE 1 FatB genes according to embodiments of the present invention Native CDS Prototheca nucloetide moriformis Sequence Amino Acid sequence (not codon- Variant (relative Sequence of codon- optimized to dominant CDS (no optimized, no nucleotide transcript additional additional sequence Species Gene Name idenitified) tags) cloning sites) of CDS Cinnamomum CcFATB1b M25L, M322R, SEQ ID NO: 1 SEQ ID NO: 2 SEQ ID camphora .DELTA.T367-D368 NO: 3 Cinnamomum CcFATB4 "wild-type" SEQ ID NO: 4 SEQ ID NO: 5 SEQ ID camphora NO: 6 Cinnamomum CcFATB3 "wild-type" SEQ ID NO: 7 SEQ ID NO: 8 SEQ ID camphora NO: 9 Cuphea ChsFATB1 "wild-type" SEQ ID NO: SEQ ID NO: 11 SEQ ID hyssopifolia 10 NO: 12 Cuphea ChsFATB2 "wild-type" SEQ ID NO: SEQ ID NO: 14 SEQ ID hyssopifolia 13 NO: 15 Cuphea ChsFATB2b +a.a.248-259 SEQ ID NO: SEQ ID NO: 17 SEQ ID hyssopifolia 16 NO: 18 Cuphea ChsFATB3 "wild-type" SEQ ID NO: SEQ ID NO: 20 SEQ ID hyssopifolia 19 NO: 21 Cuphea ChsFATB3b V204I, C239F, SEQ ID NO: SEQ ID NO: 23 SEQ ID hyssopifolia E243D, M251V 22 NO: 24 Cuphea CuPSR23FATB3 "wild-type" SEQ ID NO: SEQ ID NO: 26 SEQ ID PSR23 25 NO: 27 Cuphea CwFATB3 "wild-type" SEQ ID NO: SEQ ID NO: 29 SEQ ID wrightii 28 NO: 30 Cuphea CwFATB4a "wild-type" SEQ ID NO: SEQ ID NO: 32 SEQ ID wrightii 31 NO: 33 Cuphea CwFATB4b "wild-type" SEQ ID NO: SEQ ID NO: 35 SEQ ID wrightii 34 NO: 36 Cuphea CwFATB5 "wild-type" SEQ ID NO: SEQ ID NO: 38 SEQ ID wrightii 37 NO: 39 Cuphea ChtFATB1a "wild-type" SEQ ID NO: SEQ ID NO: 41 SEQ ID heterophylla 40 NO: 42 Cuphea ChtFATB1b P16S, T20P, G94S, SEQ ID NO: SEQ ID NO: 44 SEQ ID heterophylla G105W, S293F, 43 NO: 45 L305F Cuphea ChtFATB2b "wild-type" SEQ ID NO: SEQ ID NO: 47 SEQ ID heterophylla 46 NO: 48 Cuphea ChtFATB2a S17P, P21S, T28N, SEQ IDO NO: SEQ ID NO: 50 SEQ ID heterophylla L30P, S33L, 49 NO: 51 G76D, S78P, G137W Cuphea ChtFATB2c G76D, S78P SEQ ID NO: SEQ ID NO: 53 SEQ ID heterophylla 52 NO: 54 Cuphea ChtFATB2d S21P, T28N, SEQ ID NO: SEQ ID NO: 56 SEQ ID heterophylla L30P, S33L, 55 NO: 57 G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A Cuphea ChtFATB2e G76D, R97L, SEQ ID NO: SEQ ID NO: 59 SEQ ID heterophylla H124L, I132S, 58 NO: 60 G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A Cuphea ChtFATB2f R97L, H124L, SEQ ID NO: SEQ ID NO: 62 SEQ ID heterophylla I132S, G152S, 61 NO: 63 H165L, T211N Cuphea ChtFATB2g A6T, A16V, S17P, SEQ ID NO: SEQ ID NO: 65 SEQ ID heterophylla G76D, R97L, 64 NO: 66 H124L, I132S, S143I, G152S, A157T, H165L, T211N, G414A Cuphea ChtFATB3a "wild-type" SEQ ID NO: SEQ ID NO: 68 SEQ ID heterophylla 67 NO: 69 Cuphea ChtFATB3b C67G, H72Q, SEQ ID NO: SEQ ID NO: 71 SEQ ID heterophylla L28F, N179I 70 NO: 72 Cuphea CvisFATB1 published SEQ ID NO: N/A SEQ ID viscosissima 73 NO: 74 Cuphea CvisFATB2 published SEQ ID NO: N/A SEQ ID viscosissima 75 NO: 76 Cuphea CvisFATB3 published SEQ ID NO: N/A SEQ ID viscosissima 77 NO: 78

TABLE-US-00002 TABLE 2 Preferred codon usage in Prototheca strains Ala GCG 345 (0.36) ASN AAT 8 (0.04) GCA 66 (0.07) AAC 201 (0.96) GCT 101 (0.11) GCC 442 (0.46) Cys TGT 12 (0.10) Pro CCG 161 (0.29) TGC 105 (0.90) CCA 49 (0.09) CCT 71 (0.13) CCC 267 (0.49) Asp GAT 43 (0.12) Gln CAG 226 (0.82) GAC 316 (0.88) CAA 48 (0.18) Glu GAG 377 (0.96) Arg AGG 33 (0.06) GAA 14 (0.04) AGA 14 (0.02) CGG 102 (0.18) CGA 49 (0.08) CGT 51 (0.09) CGC 331(0.57) Phe TTT 89 (0.29) Ser AGT 16 (0.03) TTC 216 (0.71) AGC 123 (0.22) TCG 152 (0.28) TCA 31 (0.06) TCT 55 (0.10) TCC 173 (0.31) Gly GGG 92 (0.12) Thr ACG 184 (0.38) GGA 56 (0.07) ACA 24 (0.05) GGT 76 (0.10) ACT 21 (0.05) GGC 559 (0.71) ACC 249 (0.52) His CAT 42 (0.21) Val GTG 308 (0.50) CAC 154 (0.79) GTA 9 (0.01) GTT 35 (0.06) GTC 262 (0.43) Ile ATA 4 (0.01) Trp TGG 107 (1.00) ATT 30 (0.08) ATC 338 (0.91) Lys AAG 284 (0.98) Tyr TAT 10 (0.05) AAA 7 (0.02) TAC 180 (0.95) Leu TTG 26 (0.04) Stop TGA/TAG/TAA TTA 3 (0.00) CTG 447 (0.61) CTA 20 (0.03) CTT 45 (0.06) CTC 190 (0.26) Met ATG 191 (1.00)

TABLE-US-00003 TABLE 3 Preferred codon usage in Chlorella protothecoides TTC (Phe) TAC (Tyr) TGC (Cys) TGA (Stop) TGG (Trp) CCC (Pro) CAC (His) CGC (Arg) CTG (Leu) CAG (Gln) ATC (Ile) ACC (Thr) GAC (Asp) TCC (Ser) ATG (Met) AAG (Lys) GCC (Ala) AAC (Asn) GGC (Gly) GTG (Val) GAG (Glu)

Host Cells

[0026] The host cell can be a single cell or part of a multicellular organism such as a plant. Methods for expressing Fatb genes in a plant are given in U.S. Pat. Nos. 5,850,022; 5,723,761; 5,639,790; 5,807,893; 5,455,167; 5,654,495; 5,512,482; 5,298,421; 5,667,997; and U.S. Pat. Nos. 5,344,771; 5,304,481, or can be accomplished using other techniques generally known in plant biotechnology. Engineering of oleaginous microbes including Chlorophyta is disclosed in WO2010/063032, WO2011,150411, and WO2012/106560 and in the examples below.

[0027] Examples of oleaginous host cells include plant cells and microbial cells having a type II fatty acid biosynthetic pathway, including plastidic oleaginous cells such as those of oleaginous algae. Specific examples of microalgal cells include heterotrophic or obligate heterotrophic microalgae of the phylum Chlorophtya, the class Trebouxiophytae, the order Chlorellales, or the family Chlorellacae. Examples of oleaginous microalgae are provided in Published PCT Patent Applications WO2008/151149, WO2010/06032, WO2011/150410, and WO2011/150411, including species of Chlorella and Prototheca, a genus comprising obligate heterotrophs. The oleaginous cells can be, for example, capable of producing 25, 30, 40, 50, 60, 70, 80, 85, or about 90% oil by cell weight, .+-.5%. Optionally, the oils produced can be low in DHA or EPA fatty acids. For example, the oils can comprise less than 5%, 2%, or 1% DI-IA and/or EPA. The above-mentioned publications also disclose methods for cultivating such cells and extracting oil, especially from microalgal cells; such methods are applicable to the cells disclosed herein and incorporated by reference for these teachings. When microalgal cells are used they can be cultivated autotrophically (unless an obligate heterotroph) or in the dark using a sugar (e.g., glucose, fructose and/or sucrose). In any of the embodiments described herein, the cells can be heterotrophic cells comprising an exogenous invertase gene so as to allow the cells to produce oil from a sucrose feedstock. Alternately, or in addition, the cells can metabolize xylose from cellulosic feedstocks. For example, the cells can be genetically engineered to express one or more xylose metabolism genes such as those encoding an active xylose transporter, a xylulose-5-phosphate transporter, a xylose isomerase, a xylulokinase, a xylitol dehydrogenase and a xylose reductase. See WO2012/154626, "GENETICALLY ENGINEERED MICROORGANISMS THAT METABOLIZE XYLOSE", published Nov. 15, 2012.

Oils and Related Products

[0028] The oleaginous cells express one or more exogenous genes encoding fatty acid biosynthesis enzymes. As a result, some embodiments feature natural oils that were not obtainable from a non-plant or non-seed oil, or not obtainable at all.

[0029] The oleaginous cells produce a storage oil, which is primarily triacylglyceride and may be stored in storage bodies of the cell. A raw oil may be obtained from the cells by disrupting the cells and isolating the oil. WO2008/151149, WO2010/06032, WO2011/150410, and WO2011/1504 disclose heterotrophic cultivation and oil isolation techniques. For example, oil may be obtained by cultivating, drying and pressing the cells. The oils produced may be refined, bleached and deodorized (RBD) as known in the art or as described in WO2010/120939. The raw or RBD oils may be used in a variety of food, chemical, and industrial products or processes. After recovery of the oil, a valuable residual biomass remains. Uses for the residual biomass include the production of paper, plastics, absorbents, adsorbents, as animal feed, for human nutrition, or for fertilizer.

[0030] Where a fatty acid profile of a triglyceride (also referred to as a "triacylglyceride" or "TAG") cell oil is given here, it will be understood that this refers to a nonfractionated sample of the storage oil extracted from the cell analyzed under conditions in which phospholipids have been removed or with an analysis method that is substantially insensitive to the fatty acids of the phospholipids (e.g. using chromatography and mass spectrometry). The oil may be subjected to an RBD process to remove phospholipids, free fatty acids and odors yet have only minor or negligible changes to the fatty acid profile of the triglycerides in the oil. Because the cells are oleaginous, in some cases the storage oil will constitute the bulk of all the TAGs in the cell.

[0031] The stable carbon isotope value .delta.13C is an expression of the ratio of 13C/12C relative to a standard (e.g. PDB, carbonite of fossil skeleton of Belemnite americana from Peedee formation of South Carolina). The stable carbon isotope value .delta.13C (0/00) of the oils can be related to the .delta.13C value of the feedstock used. In some embodiments, the oils are derived from oleaginous organisms heterotrophically grown on sugar derived from a C4 plant such as corn or sugarcane. In some embodiments the .delta.13C (0/00) of the oil is from -10 to -17 0/00 or from -13 to -16 0/00.

[0032] The oils produced according to the above methods in some cases are made using a microalgal host cell. As described above, the microalga can be, without limitation, fall in the classification of Chlorophyta, Trebouxiophyceae, Chlorellales, Chlorellaceae, or Chlorophyceae. It has been found that microalgae of Trebouxiophyceae can be distinguished from vegetable oils based on their sterol profiles. Oil produced by Chlorella protothecoides was found to produce sterols that appeared to be brassicasterol, ergosterol, campesterol, stigmasterol, and .beta.-sitosterol, when detected by GC-MS. However, it is believed that all sterols produced by Chlorella have C24.beta. stereochemistry. Thus, it is believed that the molecules detected as campesterol, stigmasterol, and .beta.-sitosterol, are actually 22,23-dihydrobrassicasterol, proferasterol and clionasterol, respectively. Thus, the oils produced by the microalgae described above can be distinguished from plant oils by the presence of sterols with C24.beta. stereochemistry and the absence of C24.alpha. stereochemistry in the sterols present. For example, the oils produced may contain 22,23-dihydrobrassicasterol while lacking campesterol; contain clionasterol, while lacking in .beta.-sitosterol, and/or contain poriferasterol while lacking stigmasterol. Alternately, or in addition, the oils may contain significant amounts of .DELTA..sup.7-poriferasterol.

[0033] In embodiments of the present invention, oleaginous cells expressing one or more of the genes of Table 1 can produce an oil with at least 20% of C8, C10, C12, C14 or C16 fatty acids. In a specific embodiment, the level of myristate (C14:0) in the oil is greater than 30%.

[0034] Thus, in embodiments of the invention, there is a process for producing an oil, triglyceride, fatty acid, or derivative of any of these, comprising transforming a cell with any of the nucleic acids discussed herein. In another embodiment, the transformed cell is cultivated to produce an oil and, optionally, the oil is extracted. Oil extracted in this way can be used to produce food, oleochemicals or other products.

[0035] The oils discussed above alone or in combination are useful in the production of foods, fuels and chemicals (including plastics, foams, films, etc). The oils, triglycerides, fatty acids from the oils may be subjected to C--H activation, hydroamino methylation, methoxy-carbonation, ozonolysis, enzymatic transformations, epoxidation, methylation, dimerization, thiolation, metathesis, hydro-alkylation, lactonization, or other chemical processes.

[0036] After extracting the oil, a residual biomass may be left, which may have use as a fuel, as an animal feed, or as an ingredient in paper, plastic, or other product. For example, residual biomass from heterotrophic algae can be used in such products.

[0037] The described embodiments of the invention are intended to be merely exemplary and numerous variations and modifications will be apparent to those skilled in the art. All such variations and modifications are intended to be within the scope of the present invention. For example, the various triglyceride oils can be tailored in for a mixture of midchain and long chain fatty acids in order to adjust parameters such as polarity, solvency, and foam-height of the oils or chemicals made from the oils.

Example 1

[0038] Sequences of novel plant acyl-ACP thioesterases involved in seed-specific midchain (C8-C16) fatty acid biosynthesis in higher plants were isolated. Seed-specific lipid production genes were isolated through direct interrogation of RNA pools accumulating in oilseeds. Based on phylogenetic analysis, novel enzymes can be classified as members of FatB family of acyl-ACP thioesterases.

[0039] Seeds of oleaginous plants were obtained from local grocery stores or requested through USDA ARS National Plant Germplasm System (NPGS) from North Central Regional Plant Introduction Station (NCRIS) or USDA ARS North Central Soil Conservation Research Laboratory (Morris, Mich.). Dry seeds were homogenized in liquid nitrogen to powder, resuspended in cold extraction buffer containing 6-8M Urea and 3M LiCl and left on ice for a few hours to overnight at 4.degree. C. The seed homogenate was passed through NucleoSpin Filters (Macherey-Nagel) by centrifugation at 20,000 g for 20 minutes in the refrigerated microcentrifuge (4.degree. C.). The resulting RNA pellets were resuspended in the buffer containing 20 mM Tris HCl, pH7.5, 0.5% SDS, 100 mM NaCl, 25 mM EDTA, 2% PVPP) and RNA was subsequently extracted once with Phenol-Chloroform-Isoamyl Alcohol (25:24:1, v/v) and once with chloroform. RNA was finally precipitated with isopropyl alcohol (0.7 Vol.) in the presence of 150 mM of Na Acetate, pH5.2, washed with 80% ethanol by centrifugation, and dried. RNA samples were treated with Turbo DNAse (Lifetech) and purified further using RNeasy kits (Qiagen) following manufacturers' protocols. The resulting purified RNA samples were converted to pair-end cDNA libraries and subjected to next-generation sequencing (2.times.100 bp) using Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages. Putative thioesterase-containg cDNA contigs were identified by mining transcriptomes for sequences with homology to known thioesterases. These in silico identified putative thioesterase cDNAs have been further verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes.

[0040] To interrogate evolutionary and functional relationship between novel acyl-ACP thioesterases and the members of two existing thioesterase classes (FatA and FatB), we performed a phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) amino acid thioesterase sequences. Novel proteins appear to group with known acyl-ACP FatB thioesterases involved in biosynthesis of C8-C16 fatty acids. Moreover, novel thioesterases appear to cluster into 3 predominant out-groups suggesting distinct functional similarity and evolutionary relatedness among members of each cluster.

[0041] The amino acid sequences of the FatB genes follow are shown in Table 4.

TABLE-US-00004 TABLE 4 Amino acid sequences of FatB genes CuPSR23 FATB3 (SEQ ID NO: 25): MVVAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAH PKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLD RKSKRPDMLVDSVGLKCIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCK SLGLLNDGFGRTPGMCKNDLIWVLTKMQIMVNRYPTWGDTVEINTWFSQSGKIGMASD WLISDCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLH KFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRR ECGMDSVLESVTAVDPSENGGRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTST AKTSNGNSVS CuPSR23 FATB3b (SEQ ID NO: 79): MVVAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAH PKANGSAVNLKSGSLNTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLD RKSKRPDMLVDSVGLKSIVRDGLVSRQSFLIRSYEIGADRTASIETLMNHLQETSINHCKS LGLLNDGFGRTPGMCKNDLIWVLTKMQIIVIVNRYPTWGDTVEINTWFSQSGKIGMASD WLISDCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSPHVIEDNDQKLH KFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRR ECGMDSVLESVTAVDPSENGGRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTST AKTSNGNSAS CwFATB3 (SEQ ID NO: 28): MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSP HPKANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLD RKSKRPDMLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRD WLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRR ECGRESVVESVTSMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST CwFATB3a (SEQ ID NO: 28): MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSP HPKANGSAVSLKSGSLNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLD RKSKRPDMLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRD WLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRR ECGRESVVESVTSMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST CwFATB3b (SEQ ID NO: 80): MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSP HPKANGSAVSLKSGSLNTLEDLPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLD RKSKRPDMLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRD WLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILEKFWRPRSYALSPLNIGGNVE GKVW CwFATB3c (SEQ ID NO: 81): MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSP HPKANGSAVSLKSGSLNTLEDLPSSPPPRTFLNQLPDWSRLRTAITTVFVATEKQFTRLD RKSKRPDMLVDWFGSETIVQDGLVFRERFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYPTWGDTVEINSWFSQSGKIGMGRD WLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILEKFWRPRSYALSPLNIGGNVE GKVW CwFATB4a (SEQ ID NO: 31): MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSAEGYASRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSE ESSPGDFF CwFATB4a.1 (SEQ ID NO: 82): MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSAEGYASRFQHLLRLEDGGEIVKARTEWRPKNAGINWVVPSE ESSPGDFF CwFATB4a.2 (SEQ ID NO: 83): MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSAEGYASRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSE ESSPGDFF CwFATB4a.3 (SEQ ID NO: 84): MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGGFKTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSAEGYVSRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSE ESSPGDFF CwFATB4b (SEQ ID NO: 34): MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGSFKTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKI AGLSSDGFGRTPAMSKRDLIWVVAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGEGDGSKFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPS EESSPGGDFF CwFATB4b.1 (SEQ ID NO: 85): MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPP KINGSSVGLKSGSFKTQEDAPSAPPPRTFrNQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVIVINHLQETALNHVKI AGLSSDGFGRTPAMSKRDLIWVVAKMQVMVNRYPAWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSAPVVEDDDRK LPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGEGDGSKFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPS EESSPGGDFF CwFATB5 (SEQ ID NO: 37): MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPK ANGSAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRI FQDGVFFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRD LIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWA MMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWND LDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEG DRCVYQHLLRLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRVV RLIF CwFATB5a (SEQ ID NO: 86): MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPK ANGSAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRI FQDGFFFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRD LIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWA MMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWND LDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEG DRCVYQHLLRLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRVV RLIF CwFATB5b (SEQ ID NO: 87): MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPK ANGSAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRI FQDGVFFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRD LIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWA MMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWND LDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEG DRCVYQHLLWLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRV VRLIF CwFATB5c (SEQ ID NO: 88): MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPK ANGSAVNLKSGSLETPPRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRI FQDGVFFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRD LIWVVTKIQVEVNRYPIWGDTIEVNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWA MMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGDSIRDGLTPRWND LDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKEG DRCVYQHLLRLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGMGWQPFRVV RLIF CwFATB5.1 (SEQ ID NO: 89): MVAAAASSAFFSVPTPGTSPKPGKFRNWPSSLSVPFKPETNHNGGFHIKANASAH PKANGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGM LVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGE ILIRATSVWAMMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDSIR DGLTPRWNDLDVNQHVNNVKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLES VTAMDPAKEGDRSVYQHLLRLEDGADITIGRTEWRPKNAGANEAISSGKTSNGNSAS CwFATB5.1a (SEQ ID NO: 90): MVAAAASSAFFSVPTPGTSPKPGKFRNWPLSLSVPFKPETNHNGGFHIKANASAH PKANGSALNLKSGSLETQEDTSLSSPPRTFIKQLPDWSMLLSKITTVFGAAEKQLKRPGM LVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTAKETLMNIFQETSLNHCKSIGLLNDGFG RTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCRTGE ILIRATSVWAMMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDRKLYKLNVKTGDS1R DGLTPRWNDLDVNQHVNNVKFIGWILKSVPTKVFETQELCGVTLEYRRECGKDSVLES VTAMDPAKEGDRSVYQHLLRLEDGADITIGRTEWRPKNAGANEAISSGKTSNGNSAS CcFATB2b (SEQ ID NO: 91): MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKD TEGLKGCSTLQGQSMLDDHFGLHGLVFRRTFAIRCYEVGPDRSTSIMAVMNHLQEAAR NHAESLGLLGDGFGETLEMSKRDLIWVVRRTHVAVERYPAWGDTVEVEAWVGASGNT GMRRDFLVRDCKTGHILTRCTSVSVMMNMRTRRLSKIPQEVRAEIDPLFIEKVAVKEGEI KKLQKLNDSTADYIQGGWTPRWNDLDVNQHVNNIIYVGWIFKSVPDSISENHHLSSITLE YRRECIRGNKLQSLTTVCGGSSEAGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISER FPQQEPHK CcFATB3 (SEQ ID NO: 7): MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKAN AHASPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNL DWKPRRPDMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNH VRSAGLLGDGFGATPEMSRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNG MRRDWLVRDSQTGEILTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDN RKLQKLNENTADNVRRGLTPRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTL EYRRECGKDSVLQSMTAVSGGGSAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKS ANNSRSILEMPAESL CcFATB3b (SEQ ID NO: 92): MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKLASSSGLQVKAN AHASPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNL DWKPRRPDMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNH VRSAGLLGDGFGATPEMSRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNG MRRDWLVRDSQTGEILTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDN RKLQKLNENTADNVRRGLTPRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTL EYRRECGKDSVLQSMTAVSGGGSAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKS ANNSRSILEMPAESL CcFATB3c (SEQ ID NO: 93): MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKAN AHASPKINGSKVSTDTLKGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNL DWKPRRPDMLADPFGIGRFMQDGLIFRQHFAIRSYEIGADRTASIETLMNHLQETALNH VRSAGLLGDGFGATPEMSRRDLIWVVTRMQVLVDRYPAWGDIVEVETWVGASGKNG MRRDWLVRDSQTGEILTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDVAIIEEDN RKLQKLNENTADNVRRGLTPRWSDLDVNQHVNNAKYIGWILESAPGSILESHELSCMTL EYRRECGKDSVLQSMTAVSGGGSAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKS ANNSRSILEMPAESL ChtFATB1a (SEQ ID NO: 40): MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD SIRKGLTPRWNDLDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS ChtFATB1a.1 (SEQ ID NO: 94): MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRHSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLIGDC RTGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTG DSIRKGLTPRWNDLDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDS VLESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGALSTGKTSNGN SVS ChtFATB1a.2 (SEQ ID NO: 95): MVAAAASSAFFSVPTPGTSPKPGNFGNWPSNLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD SIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS ChtFATB1a.3 (SEQ ID NO: 96): MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND

GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD SIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSINQHLLRLEDGADITIGRTEWRPKNAGVNGAISTGKTSNENSVS ChtFATB1a.4 (SEQ ID NO: 97): MVAAAASSAFFSVPTPGTSPKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFGAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD SIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS ChtFATB1b (SEQ ID NO: 43): MVAAAASSAFFSVPTSGTSPKPGNFGNWPSSLSVPFKPESSHNGGFQVKANASA HPKANGSAVNLKSGSLETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFWAAERQWKRP GMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLND GFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNGMGRDWLISDCR TGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDDKKLHKLDVKTGD FIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSVL ESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS ChtFATB2b (SEQ ID NO: 46): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLD RKSKKPDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHCK SVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRE CGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGK TSNGNSVS ChtFATB2a (SEQ ID NO: 49): MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANAS AHPKANGSAVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTML DRKSKKPDMHVDWFGLEIIVQDWLVFRESFSIRSYEIGADRTASIETLMNHLQDTSLNHC KSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGR NWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPLIEDNDRKLH KFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRR ECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTG KTSNGNSVS ChtFATB2c (SEQ ID NO: 52): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTML DRKSKKPDMHVDWFGLEIIVQDGLVFRESFSIRSYEIGADRTASIETININHLQDTSLNHC KSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGR NWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRR ECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTG KTSNGNSVS ChtFATB2d (SEQ ID NO: 55): MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANAS AHPKANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTML DRKSKRPDMLVDLFGLESIVQDGLVFRESYSIRSYEIGADRTASIETLMNHLQDTSLNHC KSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYPTWGDTVEINSWFSQSGKIGMGR NWLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLH KFDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRR ECGRESVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTG KTSNGNSVS ChtFATB2e (SEQ ID NO: 58): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLD RKSKRPDMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCK SMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYRRE CGRDSVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGK TSNGNSVS ChtFATB2f (SEQ ID NO: 61): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLD RKSKRPDMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCK SMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRE CGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGK TSNGNSVS ChtFATB2g (SEQ ID NO: 64): MVVAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLD RKSKRPDMLVDWFGLESIVQDGLVFREIYSIRSYEISADRTTSIETVMNLLQETSLNHCKS MGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYRRE CGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGK TSNANSVS ChtFATB2h (SEQ ID NO: 98): MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASA HPKANGSAVSLKSGSLNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLD RKSKRPDMLVDWFGLESIVQDGLVFRESYSIRSYEISADRTASIETVMNLLQETSLNHCK SMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYPNWGDTVEINSWFSQSGKIGMGRN WLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDAPPVIEDNDRKLHK FDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESIPTEVLETQELCSLTLEYRREC GRESVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKT SNGNSVS ChtFATB3a (SEQ ID NO: 67): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASG ETSPGNS ChtFATB3b (SEQ ID NO: 70): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGFGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLIEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRR DWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWKL PKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYR RECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGE TSPGNS ChtFATB3c (SEQ ID NO: 99): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDRK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSEKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGAIAFG ETSPGDS ChtFATB3d (SEQ ID NO: 100): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSCSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIKTVMNHLQETALNHVK SAGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGM RRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDW KLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLE YRRECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIAS GETSPGNS ChtFATB3e (SEQ ID NO: 101): MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSGSLKTHEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASG ETSPGNS ChtFATB3f (SEQ ID NO: 102): MVATAASSAFFPVPSPDTSSRLGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGSSVSLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMPVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAPVIEDDDWK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSEKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASG ETSPGNS ChtFATB3g (SEQ ID NO: 103): MVATAASSAFFPVPSPDTSSRAGKLGNGSSSLRPLKPKFVANAGLQVKANASAPP KINGS SVS LKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDW KPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKS AGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMR RDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHEVDSAPVIEDDDWK LPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEY RRECGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASG ETSPGNS ChsFATB1 (SEQ ID NO: 10): MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAAR NGALPLLASICKCPKKADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAI NHVQSAGYFGDSFGATPEMTKRNLIWVITKMQVLVDRYPAWGDVVQVDTWTCSSGKN SMQRDWFVRDLKTGDIITRASSVWVLMNRLTRKLSKIPEAVLEEAKLFVMNTAPTVDD NRKLPKLDGSSADYVLSGLTPRWSDLDMNQHVNNVKYIAWILESVPQSIPETHKLSAIT VEYRRECGKNSVLQSLTNVSGDGITCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRG APIQAEKVYNNK ChsFATB2 (SEQ ID NO: 13): MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAP PKINGSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLD WKPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHV KSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNG MRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDD RKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTL EYRRECGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIA SGETSPGDSS ChsFatB2b (SEQ ID NO: 16): MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAP PKINGSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLD WKPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHV KSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNG MRRDWLISDCNTGEILTRASSKSQIMLPLHYCSVWVMMNQKTRRLSKIPDEVRHEIEPH FVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPE VLETQELCSLTLEYRRECGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEW RPKTAGINGPIASGETSPGDSS ChsFatB2c (SEQ ID NO: 104): MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAP PKINGSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLD WKPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHV KSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNG MRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDD RKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTL EYRRECGRESVLESLTAVDPSGKGSGSQFQHLMRLEDGGEIVKGRTEWRPKTAGINGPI ASGETSPGDSS ChsFatB2d (SEQ ID NO: 105): MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAP PKINGSSVGLKSGSLKTQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLD WKPKRPDMLVDPFGLGRIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHV KSAGLLNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNG MRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAPVIEDDD RKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTL EYRRECGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIA SGETSPGDSS Chs FATB3 (SEQ ID NO: 19): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFatb3b (SEQ ID NO: 22): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVINAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGR DWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLH KLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRR ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTG KTSNGNSIS ChsFatB3c (SEQ ID NO: 106): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR QECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNS IS

ChsFATB3d (SEQ ID NO: 107): MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRSDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFATB3e (SEQ ID NO: 108): MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRSDMLMDPFGVDRVVQDGVVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFATB3f (SEQ ID NO: 109): MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG, RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFATB3g (SEQ ID NO: 110): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGR DWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLH KLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQ ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS ChsFATB3h (SEQ ID NO: 111): MVAAEASSALFSVRTPGTSPKPGKFGNWPSSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDASSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRSDMLMDPFGVDRVVQDGVVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGR DWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLH KLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQ ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS ChsFATB3i (SEQ ID NO: 112): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRICSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYPTWGDTIEVNTWVSESGKTGMG RDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKL HKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYR RECGGDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAIST GKTSNGNSIS ChsFATB3j (SEQ ID NO: 113): MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASA RPKANGSAVSLKSGSLDTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTML DRKSKRPDMLMDPFGVDRVVQDGAVFRQSFSIRSYEIGADRTASIETLMNIFQETSLNHC KSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYPTWGDTIEVNTWVSESGKTGMGR DWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDSAPVIEDYQKLH KLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRQ ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGTDIAKGRTKWRPKNAGKTSNGNSIS

Example 2

[0042] In the example below, we detail the effect of expressing plant oilseed transcriptome-derived, heterologous thioesterases in the UTEX1435 (web.biosci.utexas.edu/utex/) strain, Strain A.

[0043] As in Example 1, RNA was extracted from dried plant seeds and submitted for paired-end sequencing using the Illumina Hiseq 2000 platform. RNA sequence reads were assembled into corresponding seed transcriptomes using Trinity or Oases packages and putative thioesterase-containing cDNA contigs were identified by mining transcriptomes for sequences with homology to known thioesterases. These in silico identified putative thioesterase cDNAs were verified by direct reverse transcription PCR analysis using seed RNA and primer pairs targeting full-length thioesterase cDNAs. The resulting amplified products were cloned and sequenced de novo to confirm authenticity of identified thioesterase genes and to identify sequence variants arising from expression of different gene alleles or diversity of sequences within a population of seeds. The resulting amino acid sequences were subjected to phylogenetic analysis using published full-length (Mayer and Shanklin, 2007) and truncated (THYME database) FatB sequences. The thioesterases that clustered with acyl-ACP FatB thioesterases, which are involved in biosynthesis of C8-C16 fatty acids, were pursued.

Construction of Transforming Vectors Expressing Acyl-ACP FatB Thioesterases

[0044] 27 putative acyl-ACP FatB thioesterases from the species Cinnamomum camphora, Cuphea hyssopifolia, Cuphea PSR23, Cuphea wrightii, Cuphea heterophylla, and Cuphea viscosissima were synthesized in a codon-optimized form to reflect Prototheca moriformis (UTEX 1435) codon usage. Of the 27 genes synthesized, 24 were identified by our transcriptome sequencing efforts and the 3 genes from Cuphea viscosissima, were from published sequences in GenBank.

[0045] Transgenic strains were generated via transformation of the base strain Strain A (Prototheca moriformis, derived from UTEX 1435 by classical mutation and screening for high oil production) with a construct encoding 1 of the 27 FatB thioesterases. The construct pSZ2760 encoding Cinnamomum camphora (Cc) FATB1b is shown as an example, but identical methods were used to generate each of the remaining 26 constructs encoding the different respective thioesterases. Construct pSZ2760 can be written as 6S::CrTUB2:ScSUC2:CvNR::PmAMT3:CcFATB1b:CvNR::6S. The sequence of the transforming DNA is provided in Table 5 (pSZ2760). The relevant restriction sites in the construct from 5'-3', BspQ1, KpnI, AscI, MfeI, EcoRI, SpeI, XhoI, SacI, BspQ1, respectively, are indicated in lowercase, bold, and underlined. BspQ1 sites delimit the 5' and 3' ends of the transforming DNA. Bold, lowercase sequences at the 5' and 3' end of the construct represent genomic DNA from UTEX 1435 that target integration to the 6S locus via homologous recombination. Proceeding in the 5' to 3' direction, the selection cassette has the C. reinhardtii .beta.-tubulin promoter driving expression of the S. cerevisiae gene SUC2 (conferring the ability to grow on sucrose) and the Chlorella vulgaris Nitrate Reductase (NR) gene 3' UTR. The promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for ScSUC2 are indicated by bold, uppercase italics, while the coding region is indicated with lowercase italics. The 3' UTR is indicated by lowercase underlined text. The spacer region between the two cassettes is indicated by upper case text. The second cassette containing the codon optimized CcFATB1b gene (Table 5; pSZ2760) from Cinnamomum camphora is driven by the Prototheca moriformis endogenous AMT3 promoter, and has the Chlorella vulgaris Nitrate Reductase (NR) gene 3' UTR. In this cassette, the AMT3 promoter is indicated by lowercase, boxed text. The initiator ATG and terminator TGA for the CcFATB1b gene are indicated in bold, uppercase italics, while the coding region is indicated by lowercase italics and the spacer region is indicated by upper case text. The 3' UTR is indicated by lowercase underlined text. The final construct was sequenced to ensure correct reading frame and targeting sequences.

TABLE-US-00005 TABLE 5 pSZ2760 Transforming construct (SEQ ID NO: 114) ##STR00001## ##STR00002## ##STR00003## ##STR00004## ctgcaggccttcctgttcctgctggccggcttcgccgccaagatcagcgcctccatgacgaacgagacgtccga- ccgccccctggtgca cttcacccccaacaagggctggatgaacgaccccaacggcctgtggtacgacgagaaggacgccaagtggcacc- tgtacttccagt acaacccgaacgacaccgtctgggggacgcccttgttctggggccacgccacgtccgacgacctgaccaactgg- gaggaccagccc atcgccatcgccccgaagcgcaacgactccggcgccttctccggctccatggtggtggactacaacaacacctc- cggcttcttcaacga caccatcgacccgcgccagcgctgcgtggccatctggacctacaacaccccggagtccgaggagcagtacatct- cctacagcctgga cggcggctacaccttcaccgagtaccagaagaaccccgtgctggccgccaactccacccagttccgcgacccga- aggtcttctggtac gagccctcccagaagtggatcatgaccgcggccaagtcccaggactacaagatcgagatctactcctccgacga- cctgaagtcctgg aagctggagtccgcgttcgccaacgagggcttcctcggctaccagtacgagtgccccggcctgatcgaggtccc- caccgagcaggac cccagcaagtcctactgggtgatgttcatctccatcaaccccggcgccccggccggcggctccttcaaccagta- cttcgtcggcagcttc aacggcacccacttcgaggccucgacaaccagtcccgcgtggtggacttcggcaaggactactacgccctgcag- accttcttcaaca ccgacccgacctacgggagcgccctgggcatcgcgtgggcctccaactgggagtactccgccttcgtgcccacc- aacccctggcgctc ctccatgtccctcgtgcgcaaguctccctcaacaccgagtaccaggccaacccggagacggagctgatcaacct- gaaggccgagcc gatcctgaacatcagcaacgccggcccctggagccggttcgccaccaacaccacgttgacgaaggccaacagct- acaacgtcgacc tgtccaacagcaccggcaccctggagttcgagctggtgtacgccgtcaacaccacccagacgatctccaagtcc- gtgttcgcggacct ctccctctggttcaagggcctggaggaccccgaggagtacctccgcatgggcttcgaggtgtccgcgtcctcct- tcttcctggaccgcgg gaacagcaaggtgaagttcgtgaaggagaacccctacttcaccaaccgcatgagcgtgaacaaccagcccttca- agagcgagaac gacctgtcctactacaaggtgtacggcttgctggaccagaacatcctggagctgtacttcaacgacggcgacgt- cgtgtccaccaacac ctacttcatgaccaccgggaacgccctgggctccgtgaacatgacgacgggggtggacaacctgttctacatcg- acaagttccaggtg ##STR00005## ##STR00006## ##STR00007## ##STR00008## catgaaggccgtgatgctggcccgcgacggccgcggcctgaagccccgctcctccgacctgcagctgcgcgccg- gcaacgcccaga cctccctgaagatgatcaacggcaccaagttctcctacaccgagtccctgaagaagctgcccgactggtccatg- ctgttcgccgtgatc accaccatcttctccgccgccgagaagcagtggaccaacctggagtggaagcccaagcccaaccccccccagct- gctggacgacca cttcggcccccacggcctggtgttccgccgcaccttcgccatccgctcctacgaggtgggccccgaccgctcca- cctccatcgtggccgt gatgaaccacctgcaggaggccgccctgaaccacgccaagtccgtgggcatcctgggcgacggcttcggcacca- ccctggagatgt ccaagcgcgacctgatctgggtggtgaagcgcacccacgtggccgtggagcgctaccccgcctggggcgacacc- gtggaggtgga gtgctgggtgggcgcctccggcaacaacggccgccgccacgacttcctggtgcgcgactgcaagaccggcgaga- tcctgacccgct gcacctccctgtccgtgatgatgaacacccgcacccgccgcctgtccaagatccccgaggaggtgcgcggcgag- atcggccccgcct tcatcgacaacgtggccgtgaaggacgaggagatcaagaagccccagaagctgaacgactccaccgccgactac- atccagggcg gcctgaccccccgctggaacgacctggacatcaaccagcacgtgaacaacatcaagtacgtggactggatcctg- gagaccgtgccc gactccatcttcgagtcccaccacatctcctccttcaccatcgagtaccgccgcgagtgcacccgcgactccgt- gctgcagtccctgacc accgtgtccggcggctcctccgaggccggcctggtgtgcgagcacctgctgcagctggagggcggctccgaggt- gctgcgcgccaag accgagtggcgccccaagctgtccttccgcggcatctccgtgatccccgccgagtcctccgtgatggactacaa- ggaccacgacggcg ##STR00009## ##STR00010##

[0046] Constructs encoding the identified heterologous FatB genes, such as CcFATB1b from pSZ2760 in Table 6, were transformed into Strain A, and selected for the ability to grow on sucrose. Transformations, cell culture, lipid production and fatty acid analysis were all carried out as previously described. After cultivating on sucrose under low nitrogen conditions to accumulate oil, fatty acid profiles were determined by FAME-GC. The top performer from each transformation, as judged by the ability to produce the highest level of midchain fatty acids, is shown in Table 4.

TABLE-US-00006 TABLE 6 Alteration of Fatty Acid Profiles in S3150 upon Expression of Heterologous FatB Thioesterases FA profile of top performer from each transformation (%; primary lipid in Strain A background) Species Gene Name SZ Plasmid Strain C8:0 C10:0 C12:0 Cinnamomum camphora CcFATB1b pSZ2760 A; T526; D1670-13 0 0 1 Cinnamomum camphora CcFATB4 pSZ2756 A; T525; D1666-31 0 1 33 Cinnamomum camphora CcFATB3 pSZ2755 A; T525; D1665-4 0 0 0 Cuphea hyssopifolia ChsFATB1 pSZ2778 A; T535; D1689-30 0 0 0 Cuphea hyssopifolia ChsFATB2 pSZ2796 A; T537; D1700-46 0 0 0 Cuphea hyssopifolia ChsFATB2b pSZ2792 A; T537; D1696-9 0 0 0 Cuphea hyssopifolia ChsFATB3 pSZ2797 A; T537; D1701-48 0 0 8 Cuphea hyssopifolia ChsFATB3b pSZ2795 A; T537; D1699-1 0 0 7 Cuphea PSR23 CuPSR23FATB3 pSZ2793 A; T537; D1697-13 0 1 0 Cuphea wrightii CwFATB3 pSZ2751 A; T525; D1661-22 0 2 17 Cuphea wrightii CwFATB4a pSZ2752 A; T525; D1662-30 0 0 0 Cuphea wrightii CwFATB4b pSZ2753 A; T525; D1663-29 0 0 0 Cuphea wrightii CwFATB5 pSZ2754 A; T525; D1664-39 0 0 0 Cuphea heterophylla ChtFATB1a pSZ2757 A; T525; D1667-19 0 0 5 Cuphea heterophylla ChtFATB1b pSZ2773 A; T535; D1685-29 0 0 2 Cuphea heterophylla ChtFATB2b pSZ2780 A; T535; D1691-8 0 0 0 Cuphea heterophylla ChtFATB2a pSZ2774 A; T537; D1702-24 0 0 0 Cuphea heterophylla ChtFATB2c pSZ2758 A; T525; D1668-22 0 0 3 Cuphea heterophylla ChtFATB2d pSZ2759 A; T526; D1669-19 0 0 4 Cuphea heterophylla ChtFATB2e pSZ2775 A; T535; D1686-23 0 1 2 Cuphea heterophylla ChtFATB2f pSZ2777 A; T535; D1688- 33 0 0 0 Cuphea heterophylla ChtFATB2g pSZ2794 A; T537; D1698-19 0 0 0 Cuphea heterophylla ChtFATB3a pSZ2776 A; T535; D1687-23 0 0 0 Cuphea heterophylla ChtFATB3b pSZ2779 A; T535; D1690- 31 0 0 0 Cuphea viscosissima CvisFATB1 pSZ2810 A; T540; D1711-30 0 1 0 Cuphea viscosissima CvisFATB2 pSZ2817 A; T547; D1718-1 0 0 0 Cuphea viscosissima CvisFATB3 pSZ2791 A; T537; D1695-1 0 0 0 A (parent strain): 0 0 0 FA profile of top performer from each transformation (%; primary lipid in Strain A background) Species C14:0 C16:0 C18:0 C18:1 C18:2 C18:3.alpha. Cinnamomum camphora 15 26 2 46 9 1 Cinnamomum camphora 4 7 2 41 10 1 Cinnamomum camphora 3 44 3 41 8 0 Cuphea hyssopifolia 2 22 4 63 8 1 Cuphea hyssopifolia 6 53 3 32 6 0 Cuphea hyssopifolia 5 26 2 56 9 1 Cuphea hyssopifolia 34 27 2 24 5 1 Cuphea hyssopifolia 29 27 1 28 6 1 Cuphea PSR23 2 24 3 61 8 1 Cuphea wrightii 9 19 2 41 8 1 Cuphea wrightii 4 48 3 36 7 1 Cuphea wrightii 5 52 3 32 6 1 Cuphea wrightii 3 27 3 57 7 1 Cuphea heterophylla 18 27 2 39 7 1 Cuphea heterophylla 7 27 3 53 8 1 Cuphea heterophylla 2 25 3 61 8 1 Cuphea heterophylla 2 27 3 59 6 0 Cuphea heterophylla 2 23 3 58 7 1 Cuphea heterophylla 4 23 3 54 9 1 Cuphea heterophylla 3 24 3 57 8 1 Cuphea heterophylla 2 28 3 57 8 1 Cuphea heterophylla 2 22 3 62 9 1 Cuphea heterophylla 5 47 4 37 7 1 Cuphea heterophylla 6 49 5 32 7 0 Cuphea viscosissima 2 24 3 60 8 0 Cuphea viscosissima 4 51 2 36 6 0 Cuphea viscosissima 8 28 2 52 8 1 2 28 3 58 7 0

[0047] Many of the acyl-ACP FatB thioesterases were found to exhibit midchain activity when expressed in Prototheca moriformis. For example, expression of CcFATB1b causes an increase in myristate levels from 2% of total fatty acids in the parent, Strain A, to .about.15% in the D1670-13 primary transformant. Other examples include CcFATB4, which exhibits an increase in laurate levels from 0% in Strain A to .about.33%, and ChsFATB3, which exhibits an increase in myristate levels to .about.34%. Although some of the acyl-ACP thioesterases did not exhibit dramatic effects on midchain levels in the current incarnation, efforts will likely develop to optimize some of these constructs.

Sequences of the Heterologous Acyl-ACP Thioesterases Identified and Transformed into P. moriformis (UTEX 1435)

[0048] A complete listing of relevant sequences for the transforming constructs, such as the deduced amino acid sequence of the encoded acyl-ACP thioesterase, the native CDS coding sequence, the Prototheca moriformis codon-optimized coding sequence, and the nature of the sequence variants examined, is provided as SEQ ID NOS: 1-78.

TABLE-US-00007 Sequence Listing SEQ ID NO 1: Cinnamomum camphora (Cc) FATB1b variant M25L, M322R, AT367-D368 amino acid sequence MATTSLASAFCSMKAVMLARDGRGLKPRSSDLQLRAGNAQTSLKMINGTKFSYTESLKKLPDWSMLFAVI TTIFSAAEKQWTNLEWKPKPNPPQLLDDHFGPHGLVFRRTFAIRSYEVGPDRSTSIVAVMNHLQEAALNH AKSVGILGDGFGTTLEMSKRDLIWVVKRTHVAVERYPAWGDTVEVECWVGASGNNGRRHDFLVRDCKTGE ILTRCTSLSVMMNTRTRRLSKIPEEVRGEIGPAFIDNVAVKDEEIKKPQKLNDSTADYIQGGLTPRWNDL DINQHVNNIKYVDWILETVPDSIFESHHISSFTIEYRRECTRDSVLQSLTTVSGGSSEAGLVCEHLLQLE GGSEVLRAKTEWRPKLSFRGISVIPAESSV* SEQ ID NO 2: Cinnamomum camphora (Cc) FATB1b variant M25L, M322R, 6T367-D368 coding DNA sequence TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGAAACCCAGGA GCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGATCAATGGGACCAAGTTCAG TTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGCTCTTTGCAGTGATCACGACCATCTTTTCG GCTGCTGAGAAGCAGTGGACCAATCTAGAGTGGAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACC ATTTTGGGCCGCATGGGTTAGTTTTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCG CTCCACATCTATAGTGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGA ATTCTAGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAAACGCA CGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTGCTGGGTTGGTGCATC GGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAAAACAGGCGAAATTCTTACAAGATGT ACCAGTCTTTCGGTGATGATGAATACAAGGACAAGGAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGG AGATAGGGCCTGCATTCATTGATAATGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAA TGACAGCACTGCAGATTACATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGATATCAATCAGCAC GTTAACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCATCATA TTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCAGTCCCTGACCACTGT CTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTCCAGCTTGAAGGTGGGTCTGAGGTA TTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGTTTCAGAGGGATTAGTGTGATACCCGCAGAATCGA GTGTCTAA SEQ ID NO 3: Cinnamomum camphora (Cc) FATB1b variant M25L, M322R, AT367-D368 coding DNA sequence codon optimized for Prototheca moriformis TTAGCTTCTGCTTTCTGCTCGATGAAAGCTGTAATGTTGGCTCGTGATGGCAGGGGCTTGAAACCCAGGA GCAGTGATTTGCAGCTGAGGGCGGGAAATGCACAAACCTCTTTGAAGATGATCAATGGGACCAAGTTCAG TTACACAGAGAGCTTGAAAAAGTTGCCTGACTGGAGCATGCTCTTTGCAGTGATCACGACCATCTTTTCG GCTGCTGAGAAGCAGTGGACCAATCTAGAGTGGAAGCCGAAGCCGAATCCACCCCAGTTGCTTGATGACC ATTTTGGGCCGCATGGGTTAGTTTTCAGGCGCACCTTTGCCATCAGATCGTATGAGGTGGGACCTGACCG CTCCACATCTATAGTGGCTGTTATGAATCACTTGCAGGAGGCTGCACTTAATCATGCGAAGAGTGTGGGA ATTCTAGGAGATGGATTCGGTACGACGCTAGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAAACGCA CGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGTGATACTGTTGAAGTAGAGTGCTGGGTTGGTGCATC GGGAAATAATGGCAGGCGCCATGATTTCCTTGTCCGGGACTGCAAAACAGGCGAAATTCTTACAAGATGT ACCAGTCTTTCGGTGATGATGAATACAAGGACAAGGAGGTTGTCCAAAATCCCTGAAGAAGTTAGAGGGG AGATAGGGCCTGCATTCATTGATAATGTGGCTGTCAAGGACGAGGAAATTAAGAAACCACAGAAGCTCAA TGACAGCACTGCAGATTACATCCAAGGAGGATTGACTCCTCGATGGAATGATTTGGATATCAATCAGCAC GTTAACAACATCAAATACGTTGACTGGATTCTTGAGACTGTCCCAGACTCAATCTTTGAGAGTCATCATA TTTCCAGCTTCACTATTGAATACAGGAGAGAGTGCACGAGGGATAGCGTGCTGCAGTCCCTGACCACTGT CTCCGGTGGCTCGTCGGAAGCTGGGTTAGTGTGCGAGCACTTGCTCCAGCTTGAAGGTGGGTCTGAGGTA TTGAGGGCAAAAACAGAGTGGAGGCCTAAGCTTAGTTTCAGAGGGATTAGTGTGATACCCGCAGAATCGA GTGTCTAA SEQ ID NO: 4 Cinnamomum camphora (Cc) FATB4 amino acid sequence MVTTSLASAYFSMKAVMLAPDGRGIKPRSSGLQVRAGNERNSCKVINGTKVKDTEGLKGCSTLQGQSMLD DHFGLHGLVFRRTFAIRCYEVGPDRSTSIMAVMNHLQEAARNHAESLGLLGDGFGETLEMSKRDLIWVVR RTHVAVERYPAWGDTVEVEAWVGASGNTGMRRDFLVRDCKTGHILTRCTSVSVMMNMRTRRLSKIPQEVR AEIDPLFIEKVAVKEGEIKKLQKLNDSTADYIQGGWTPRWNDLDVNQHVNNIIYVGWIFKSVPDSISENH HLSSITLEYRRECTRGNKLQSLTTVCGGSSEAGIICEHLLQLEDGSEVLRARTEWRPKHTDSFQGISERF PQQEPHK SEQ ID NO: 5 Cinnamomum camphora (Cc) FATB4 coding DNA sequence ATGGTCACCACCTCTTTAGCTTCCGCTTACTTCTCGATGAAAGCTGTAATGTTGGCTCCTGACGGCAGGG GCATAAAGCCCAGGAGCAGTGGTTTGCAGGTGAGGGCGGGAAATGAACGAAACTCTTGCAAGGTGATCAA TGGGACCAAGGTCAAAGACACGGAGGGCTTGAAAGGGTGCAGCACGTTGCAAGGCCAGAGCATGCTTGAT GACCATTTTGGTCTGCATGGGCTAGTTTTCAGGCGCACCTTTGCAATCAGATGCTATGAGGTTGGACCTG ACCGCTCCACATCCATAATGGCTGTTATGAATCACTTGCAGGAAGCTGCACGTAATCATGCGGAGAGTCT GGGACTTCTAGGAGATGGATTCGGTGAGACACTGGAGATGAGTAAGAGAGATCTGATATGGGTTGTGAGA CGCACGCATGTTGCTGTGGAACGGTACCCTGCTTGGGGCGATACTGTTGAAGTCGAGGCCTGGGTGGGTG CATCAGGTAACACTGGCATGCGCCGCGATTTCCTTGTCCGCGACTGCAAAACTGGCCACATTCTTACAAG ATGTACCAGTGTTTCAGTGATGATGAATATGAGGACAAGGAGATTGTCCAAAATTCCCCAAGAAGTTAGA GCGGAGATTGACCCTCTTTTCATTGAAAAGGTTGCTGTCAAGGAAGGGGAAATTAAAAAATTACAGAAGT TGAATGATAGCACTGCAGATTACATTCAAGGGGGTTGGACTCCTCGATGGAATGATTTGGATGTCAATCA GCACGTGAACAATATCATATACGTTGGCTGGATTTTTAAGAGCGTCCCAGACTCTATCTCTGAGAATCAT CATCTTTCTAGCATCACTCTCGAATACAGGAGAGAGTGCACAAGGGGCAACAAGCTGCAGTCCCTGACCA CTGTTTGTGGTGGCTCGTCGGAAGCTGGGATCATATGTGAGCACCTACTCCAGCTTGAGGATGGGTCTGA GGTTTTGAGGGCAAGAACAGAGTGGAGGCCCAAGCACACCGATAGTTTCCAAGGCATTAGTGAGAGATTC CCGCAGCAAGAACCGCATAAGTAA SEQ ID NO: 6 Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGACCACCTCCCTGGCCTCCGCCTACTTCTCCATGAAGGCCGTGATGCTGGCCCCCGACGGCCGCG GCATCAAGCCCCGCTCCTCCGGCCTGCAGGTGCGCGCCGGCAACGAGCGCAACTCCTGCAAGGTGATCAA CGGCACCAAGGTGAAGGACACCGAGGGCCTGAAGGGCTGCTCCACCCTGCAGGGCCAGTCCATGCTGGAC GACCACTTCGGCCTGCACGGCCTGGTGTTCCGCCGCACCTTCGCCATCCGCTGCTACGAGGTGGGCCCCG ACCGCTCCACCTCCATCATGGCCGTGATGAACCACCTGCAGGAGGCCGCCCGCAACCACGCCGAGTCCCT GGGCCTGCTGGGCGACGGCTTCGGCGAGACCCTGGAGATGTCCAAGCGCGACCTGATCTGGGTGGTGCGC CGCACCCACGTGGCCGTGGAGCGCTACCCCGCCTGGGGCGACACCGTGGAGGTGGAGGCCTGGGTGGGCG CCTCCGGCAACACCGGCATGCGCCGCGACTTCCTGGTGCGCGACTGCAAGACCGGCCACATCCTGACCCG CTGCACCTCCGTGTCCGTGATGATGAACATGCGCACCCGCCGCCTGTCCAAGATCCCCCAGGAGGTGCGC GCCGAGATCGACCCCCTGTTCATCGAGAAGGTGGCCGTGAAGGAGGGCGAGATCAAGAAGCTGCAGAAGC TGAACGACTCCACCGCCGACTACATCCAGGGCGGCTGGACCCCCCGCTGGAACGACCTGGACGTGAACCA GCACGTGAACAACATCATCTACGTGGGCTGGATCTTCAAGTCCGTGCCCGACTCCATCTCCGAGAACCAC CACCTGTCCTCCATCACCCTGGAGTACCGCCGCGAGTGCACCCGCGGCAACAAGCTGCAGTCCCTGACCA CCGTGTGCGGCGGCTCCTCCGAGGCCGGCATCATCTGCGAGCACCTGCTGCAGCTGGAGGACGGCTCCGA GGTGCTGCGCGCCCGCACCGAGTGGCGCCCCAAGCACACCGACTCCTTCCAGGGCATCTCCGAGCGCTTC CCCCAGCAGGAGCCCCACAAGTGA SEQ ID NO: 7 Cinnamomum camphora (Cc) FATB3 amino acid sequence MVATAAASAFFPVGAPATSSATSAKASMMPDNLDARGIKPKPASSSGLQVKANAHASPKINGSKVSTDTL KGEDTLTSSPAPRTFINQLPDWSMFLAAITTIFLAAEKQWTNLDWKPRRPDMLADPFGIGRFMQDGLIFR QHFAIRSYEIGADRTASIETLMNHLQETALNHVRSAGLLGDGFGATPEMSRRDLIWVVTRMQVLVDRYPA WGDIVEVETWVGASGKNGMRRDWLVRDSQTGEILTRATSVWVMMNKRTRRLSKLPEEVRGEIGPYFIEDV AIIEEDNRKLQKLNENTADNVRRGLTPRWSDLDVNQHVNNVKYIGWILESAPGSILESHELSCMTLEYRR ECGKDSVLQSMTAVSGGGSAAGGSPESSVECDHLLQLESGPEVVRGRTEWRPKSANNSRSILEMPAESL SEQ ID NO: 8 Cinnamomum camphora (Cc) FATB3 coding DNA sequence ATGGTTGCCACCGCTGCTGCTTCTGCTTTCTTCCCGGTCGGTGCTCCGGCTACGTCATCTGCAACTTCAG CCAAAGCGTCGATGATGCCTGATAATTTGGATGCCAGAGGCATCAAACCGAAGCCGGCTTCGTCCAGCGG CTTGCAGGTTAAGGCAAATGCCCATGCCTCTCCCAAGATTAATGGTTCCAAGGTGAGCACGGATACCTTG AAGGGGGAAGACACCTTAACTTCCTCGCCCGCCCCACGGACCTTTATCAACCAATTGCCTGACTGGAGCA TGTTCCTTGCTGCCATCACAACTATTTTCTTGGCTGCCGAGAAGCAGTGGACGAATCTCGACTGGAAGCC CAGAAGACCCGACATGCTTGCTGACCCGTTTGGCATCGGGAGGTTTATGCAGGATGGGCTGATTTTCAGG CAGCACTTTGCAATCAGATCTTATGAGATTGGGGCTGATAGAACGGCGTCTATAGAGACTTTAATGAATC ACTTGCAGGAGACTGCACTTAATCATGTGAGGAGTGCTGGACTCCTAGGTGATGGATTTGGTGCGACACC TGAGATGAGTAGAAGAGATCTGATATGGGTTGTAACACGTATGCAGGTTCTTGTGGACCGCTACCCTGCT TGGGGTGATATTGTTGAAGTAGAGACCTGGGTTGGTGCATCTGGAAAAAATGGTATGCGCCGTGATTGGC TTGTTCGGGACAGCCAAACTGGTGAAATTCTCACACGAGCTACCAGTGTTTGGGTGATGATGAATAAACG GACAAGGCGATTGTCCAAACTTCCTGAAGAAGTTAGAGGGGAAATAGGGCCTTATTTTATAGAAGATGTT GCTATCATAGAGGAGGACAACAGGAAACTACAGAAGCTCAATGAAAACACTGCTGATAATGTTCGAAGGG GTTTGACTCCTCGCTGGAGTGATCTGGATGTTAATCAGCATGTGAACAATGTCAAATACATTGGTTGGAT TCTTGAGAGTGCACCAGGATCCATCTTGGAGAGTCATGAGCTTTCCTGCATGACCCTTGAATACAGGAGA GAATGTGGGAAGGACAGTGTGCTGCAGTCAATGACTGCTGTCTCTGGTGGAGGCAGTGCAGCAGGTGGCT CACCAGAATCTAGCGTTGAGTGTGACCACTTGCTCCAGCTAGAGAGTGGGCCTGAAGTTGTGAGGGGAAG AACCGAGTGGAGGCCCAAGAGTGCTAATAACTCGAGGAGCATCCTGGAGATGCCGGCCGAGAGC SEQ ID NO: 9 Cinnamomum camphora (Cc) FATB4 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCGCCTCCGCCTTCTTCCCCGTGGGCGCCCCCGCCACCTCCTCCGCCACCTCCG CCAAGGCCTCCATGATGCCCGACAACCTGGACGCCCGCGGCATCAAGCCCAAGCCCGCCTCCTCCTCCGC CCTGCAGGTGAAGGCCAACGCCCACGCCTCCCCCAAGATCAACGGCTCCAAGGTGTCCACCGACACCCTG AAGGGCGAGGACACCCTGACCTCCTCCCCCGCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCA TGTTCCTGGCCGCCATCACCACCATCTTCCTGGCCGCCGAGAAGCAGTGGACCAACCTGGACTGGAAGCC CCGCCGCCCCGACATGCTGGCCGACCCCTTCGGCATCGGCCGCTTCATGCAGGACGGCCTGATCTTCCGC CAGCACTTCGCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCGCCCTGAACCACGTGCGCTCCGCCGGCCTGCTGGGCGACGGCTTCGGCGCCACCCC CGAGATGTCCCGCCGCGACCTGATCTGGGTGGTGACCCGCATGCAGGTGCTGGTGGACCGCTACCCCGCC TGGGGCGACATCGTGGAGGTGGAGACCTGGGTGGGCGCCTCCGGCAAGAACGGCATGCGCCGCGACTGGC TGGTGCGCGACTCCCAGACCGGCGAGATCCTGACCCGCGCCACCTCCGTGTGGGTGATGATGAACAAGCG CACCCGCCGCCTGTCCAAGCTGCCCGAGGAGGTGCGCGGCGAGATCGGCCCCTACTTCATCGAGGACGTG GCCATCATCGAGGAGGACAACCGCAAGCTGCAGAAGCTGAACGAGAACACCGCCGACAACGTGCGCCGCG GCCTGACCCCCCGCTGGTCCGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCGCCCCCGGCTCCATCCTGGAGTCCCACGAGCTGTCCTGCATGACCCTGGAGTACCGCCGC GAGTGCGGCAAGGACTCCGTGCTGCAGTCCATGACCGCCGTGTCCGGCGGCGGCTCCGCCGCCGGCGGCT CCCCCGAGTCCTCCGTGGAGTGCGACCACCTGCTGCAGCTGGAGTCCGGCCCCGAGGTGGTGCGCGGCCG CACCGAGTGGCGCCCCAAGTCCGCCAACAACTCCCGCTCCATCCTGGAGATGCCCGCCGAGTCCCTGTGA SEQ ID NO: 10 Cuphea hyssopifolia (Chs) FATB1 amino acid sequence MVATNAAAFSAYTFFLTSPTHGYSSKRLADTQNGYPGTSLKSKSTPPPAAAAARNGALPLLASICKCPKK ADGSMQLDSSLVFGFQFYIRSYEVGADQTVSIQTVLNYLQEAAINHVQSAGYFGDSFGATPEMTKRNLIW VITKMQVLVDRYPAWGDVVQVDTWTCSSGKNSMQRDWFVRDLKTGDIITRASSVWVLMNRLTRKLSKIPE AVLEEAKLFVMNTAPTVDDNRKLPKLDGSSADYVLSGLTPRWSDLDMNQHVNNVKYIAWILESVPQSIPE THKLSAITVEYRRECGKNSVLQSLTNVSGDGITCGNSIIECHHLLQLETGPEILLARTEWISKEPGFRGA PIQAEKVYNNK* SEQ ID NO: 11 Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence ATGGTTGCCACTAATGCTGCTGCCTTTTCTGCTTATACTTTCTTCCTTACTTCACCAACTCATGGTTACT CTTCCAAACGTCTCGCCGATACTCAAAATGGTTATCCGGGTACCTCCTTGAAATCGAAATCCACTCCTCC ACCAGCTGCTGCTGCTGCTCGTAACGGTGCATTGCCACTGCTGGCCTCCATCTGCAAATGCCCCAAAAAG GCTGATGGGAGTATGCAACTAGACAGCTCCTTGGTCTTCGGGTTTCAATTTTACATTAGATCATATGAAG TGGGTGCGGATCAAACCGTGTCAATACAGACAGTACTCAATTACTTACAGGAGGCAGCCATCAATCATGT TCAGAGTGCTGGCTATTTTGGTGATAGTTTTGGCGCCACCCCGGAAATGACCAAGAGGAACCTCATCTGG GTTATCACTAAGATGCAGGTTTTGGTGGATCGCTATCCCGCTTGGGGCGATGTTGTTCAAGTTGATACAT GGACCTGTAGTTCTGGTAAAAACAGCATGCAGCGTGATTGGTTCGTACGGGATCTCAAAACTGGAGATAT TATAACAAGAGCCTCGAGCGTGTGGGTGCTGATGAATAGACTCACCAGAAAATTATCAAAAATTCCTGAA GCAGTTCTGGAAGAAGCAAAACTTTTTGTGATGAACACTGCCCCCACCGTAGATGACAACAGGAAGCTAC CAAAGCTGGATGGCAGCAGTGCTGATTATGTCCTCTCTGGCTTAACTCCTAGATGGAGCGACTTAGATAT GAACCAGCATGTCAACAATGTGAAGTACATAGCCTGGATCCTTGAGAGTGTCCCTCAGAGCATACCGGAG ACACACAAGCTGTCAGCGATAACCGTGGAGTACAGGAGAGAATGTGGCAAGAACAGCGTCCTCCAGTCTC TGACCAACGTCTCCGGGGATGGAATCACATGTGGAAACAGTATTATCGAGTGCCACCATTTGCTTCAACT TGAGACTGGCCCAGAGATTCTACTAGCGCGGACGGAGTGGATATCCAAGGAACCTGGGTTCAGGGGAGCT CCAATCCAGGCAGAGAAAGTCTACAACAACAAATAA SEQ ID NO: 12 Cuphea hyssopifolia (Chs) FATB1 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCAACGCCGCCGCCTTCTCCGCCTACACCTTCTTCCTGACCTCCCCCACCCACGGCTACT CCTCCAAGCGCCTGGCCGACACCCAGAACGGCTACCCCGGCACCTCCCTGAAGTCCAAGTCCACCCCCCC CCCCGCCGCCGCCGCCGCCCGCAACGGCGCCCTGCCCCTGCTGGCCTCCATCTGCAAGTGCCCCAAGAAG GCCGACGGCTCCATGCAGCTGGACTCCTCCCTGGTGTTCGGCTTCCAGTTCTACATCCGCTCCTACGAGG TGGGCGCCGACCAGACCGTGTCCATCCAGACCGTGCTGAACTACCTGCAGGAGGCCGCCATCAACCACGT GCAGTCCGCCGGCTACTTCGGCGACTCCTTCGGCGCCACCCCCGAGATGACCAAGCGCAACCTGATCTGG GTGATCACCAAGATGCAGGTGCTGGTGGACCGCTACCCCGCCTGGGGCGACGTGGTGCAGGTGGACACCT GGACCTGCTCCTCCGGCAAGAACTCCATGCAGCGCGACTGGTTCGTGCGCGACCTGAAGACCGGCGACAT CATCACCCGCGCCTCCTCCGTGTGGGTGCTGATGAACCGCCTGACCCGCAAGCTGTCCAAGATCCCCGAG GCCGTGCTGGAGGAGGCCAACCTGTTCGTGATGAACACCGCCCCCACCGTGGACGACAACCGCAAGCTGC CCAAGCTGGACGGCTCCTCCGCCGACTACGTGCTGTCCGGCCTGACCCCCCGCTGGTCCGACCTGGACAT GAACCAGCACGTGAACAACGTGAAGTACATCGCCTGGATCCTGGAGTCCGTGCCCCAGTCCATCCCCGAG ACCCACAAGCTGTCCGCCATCACCGTGGAGTACCGCCGCGAGTGCGGCAAGAACTCCGTGCTGCAGTCCC TGACCAACGTGTCCGGCGACGGCATCACCTGCGGCAACTCCATCATCGAGTGCCACCACCTGCTGCAGCT GGAGACCGGCCCCGAGATCCTGCTGGCCCGCACCGAGTGGATCTCCAAGGAGCCCGGCTTCCGCGGCGCC CCCATCCAGGCCGAGAAGGTGTACAACAACAAGTGA SEQ ID NO: 13 Cuphea hyssopifolia (Chs) FATB2 amino acid sequence MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVGLKSGSLK TQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGRIVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRHEIEPHFVDSAP VIEDDDRKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRE CGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGETSPGDSS* SEQ ID NO: 14 Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTAGACCTGGAA AGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGGCCAATGGCGGGTTGCAGGT TAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTTCGGTCGGTCTAAAGTCCGGCAGTCTCAAG ACTCAGGAAGACACTCCTTCGGCGCCTCCTCCCCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGC TTCTTGCTGCAATCACTACTGTCTTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAA GAGGCCTGACATGCTTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAG AATTTTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAACCACT TGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTTGGTCGTACTCTTGA GATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCATGGTTAACCGCTATCCTACTTGG GGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGATTGGCTCA TAAGTGATTGCAATACAGGAGAAATTCTTACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGAC AAGAAGATTGTCAAAAATTCCAGATGAGGTTCGACATGAGATAGAGCCTCATTTCGTGGACTCTGCTCCC GTCATTGAAGATGATGACCGGAAACTTCCCAAGCTGGATGAGAAGACTGCTGACTCCATCCGCAAGGGTC TAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGATTCT TGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTACCCTGGAATATAGGCGGGAA TGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTGGACCCCTCTGGAAAGGGCTCTGGGTCTCAGT TCCAGCACCTTCTGCGGCTTGAGGATGGAGGTGAGATTGTGAAGGGGAGAACTGAGTGGCGACCCAAGAC TGCAGGAATCAATGGGCCAATAGCATCCGGGGAGACCTCACCTGGAGACTCTTCTTAG SEQ ID NO: 15 Cuphea hyssopifolia (Chs) FATB2 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCCAACGGCGGCCTGCAGGT GAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCTCCCTGAAG ACCCAGGAGGACACCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAA GCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTTCGGCCGCACCCTGGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGAC CCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCC GTGATCGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCC

TGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCT GGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAG TGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCAAGGGCTCCGGCTCCCAGT TCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAC CGCCGGCATCAACGGCCCCATCGCCTCCGGCGAGACCTCCCCCGGCGACTCCTCCTGA SEQ ID NO: 16 Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant amino acid sequence MVATAASSAFFPVPSPDASSRPGKLGNGSSSLSPLKPKLMANGGLQVKANASAPPKINGSSVGLKSGSLK TQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGRIVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNDGFGRTLEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSKSQIMLPLHYCSVWVMMNQKTRRLSKIPDEVR HEIEPHFVDSAPVIEDDDRKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQ ELCSLTLEYRRECGRESVLESLTAVDPSGKGSGSQFQHLLRLEDGGEIVKGRTEWRPKTAGINGPIASGE TSPGDSS* SEQ ID NO: 17 Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant coding DNA sequence ATGGTGGCTACCGCTGCAAGTTCAGCATTCTTCCCTGTGCCGTCCCCCGACGCCTCCTCTAGACCTGGAA AGCTCGGCAATGGGTCATCGAGCTTGAGCCCCCTCAAGCCCAAATTGATGGCCAATGGCGGGTTGCAGGT TAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCTTCGGTCGGTCTAAAGTCCGGCAGTCTCAAG ACTCAGGAAGACACTCCTTCGGCGCCTCCTCCdCGGACTTTTATTAACCAGCTGCCTGATTGGAGTATGC TTCTTGCTGCAATCACTACTGTCTTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCCAA GAGGCCTGACATGCTTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAAGATGGGCTTGTGTTCAGGCAG AATTTTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAACCACT TGCAGGAAACAGCTCTCAATCATGTTAAGAGTGCTGGGCTTCTTAATGACGGCTTTGGTCGTACTCTTGA GATGTATAAAAGGGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCATGGTTAACCGCTATCCTACTTGG GGCGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGATTGGCTCA TAAGTGATTGCAATACAGGAGAAATTCTTACTAGAGCATCAAGTAAAAGCCAAATTATGTTACCCTTACA TTATTGCAGTGTGTGGGTCATGATGAATCAAAAGACAAGAAGATTGTCAAAAATTCCAGATGAGGTTCGA CATGAGATAGAGCCTCATTTCGTGGACTCTGCTCCCGTCATTGAAGATGATGACCGGAAACTTCCCAAGC TGGATGAGAAGACTGCTGACTCCATCCGCAAGGGTCTAACTCCGAAGTGGAATGACTTGGATGTCAATCA GCACGTCAACAACGTGAAGTACATTGGGTGGATTCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAG GAGTTATGTTCCCTTACCCTGGAATATAGGCGGGAATGCGGAAGGGAGAGCGTGCTGGAGTCCCTCACTG CTGTGGACCCCTCTGGAAAGGGCTCTGGGTCTCAGTTCCAGCACCTTCTGCGGCTTGAGGATGGAGGTGA GATTGTGAAGGGGAGAACTGAGTGGCGACCCAAGACTGCAGGAATCAATGGGCCAATAGCATCCGGGGAG ACCTCACCTGGAGACTCTTCTTAG SEQ ID NO: 18 Cuphea hyssopifolia (Chs) FATB2b +a.a.248-259 variant coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACGCCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCTCCTCCTCCCTGTCCCCCCTGAAGCCCAAGCTGATGGCCAACGGCGGCCTGCAGGT GAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCTCCCTGAAG ACCCAGGAGGACACCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAA GCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGACGGCTTCGGCCGCACCCTGGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCAAGTCCCAGATCATGCTGCCCCTGCA CTACTGCTCCGTGTGGGTGATGATGAACCAGAAGACCCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGC CACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGACGACCGCAAGCTGCCCAAGC TGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCCTGACCCCCAAGTGGAACGACCTGGACGTGAACCA GCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAG GAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCG CCGTGGACCCCTCCGGCAAGGGCTCCGGCTCCCAGTTCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGA GATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGACCGCCGGCATCAACGGCCCCATCGCCTCCGGCGAG ACCTCCCCCGGCGACTCCTCCTGA SEQ ID NO: 19 Cuphea hyssopifolia (Chs) FATB3 amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSAVSLKSGSL DTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLMDPFGVDRVVQDGAVF RQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKMHVEVNRYP TWGDTIEVNTWVSESGKTGMGRDWLISDCHTGEILIRATSMCAMMNQKTRRFSKFPYEVRQELAPHFVDS APVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRR ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS* SEQ ID NO: 20 Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTAAACCCGGGA AGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAAACCACAATGGCGGCTTTCA GGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTC GACACTCAGGAGGACACTTCATCGTCGTCCTCTCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGA GTATGCTGCTGTCCGCGATCACGACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAA ATCTAAGAGGCCCGACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTC AGACAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGA ACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTAC TCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAATGCACGTCGAGGTTAATCGCTATCCT ACTTGGGGTGATACTATCGAGGTCAATACTTGGGTCTCCGAGTCGGGGAAAACCGGTATGGGTCGTGATT GGCTGATAAGTGATTGTCATACAGGAGAAATTCTAATAAGAGCAACGAGCATGTGTGCTATGATGAATCA AAAGACGAGAAGATTCTCAAAATTTCCATATGAGGTTCGACAGGAGTTGGCGCCTCATTTTGTGGACTCT GCTCCTGTCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCAATG GCCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGAT TCTCGAGAGTGTTCCAACGGAAGTTTTCGAGACCCAGGAGCTATGTGGCCTCACCCTTGAGTATAGGCGG GAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATCCATCAAAAGAGGGAGACAGATCTC TGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAA GAATGCAGGAACCAATGGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG SEQ ID NO: 21 Cuphea hyssopifolia (Chs) FATB3 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGAGGCCTCCTCCGCCCTGTTCTCCGTGCGCACCCCCGGCACCTCCCCCAAGCCCGGCA AGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAACCACAACGGCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCCCTG GACACCCAGGAGGACACCTCCTCCTCCTCCTCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGT CCATGCTGCTGTCCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTC CGCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGCACGTGGAGGTGAACCGCTACCCC ACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACT GGCTGATCTCCGACTGCCACACCGGCGAGATCCTGATCCGCGCCACCTCCATGTGCGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGTTCCCCTACGAGGTGCGCCAGGAGCTGGCCCCCCACTTCGTGGACTCC GCCCCCGTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGCAACG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCCTGACCCTGGAGTACCGCCGC GAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCC TGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAA GAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA SEQ ID NO: 22 Cuphea hyssopifolia (Chs) FATB3b (V204I, C239F, E243D, M251V variant) amino acid sequence MVAAEASSALFSVRTPGTSPKPGKFGNWPTSLSVPFKSKSNHNGGFQVKANASARPKANGSAVSLKSGSL DTQEDTSSSSSPPRTFINQLPDWSMLLSAITTVFVAAEKQWTMLDRKSKRPDMLMDPFGVDRVVQDGAVF RQSFSIRSYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKMHIEVNRYP TWGDTIEVNTWVSESGKTGMGRDWLISDFHTGDILIRATSVCAMMNQKTRRFSKFPYEVRQELAPHFVDS APVIEDYQKLHKLDVKTGDSICNGLTPRWNDLDVNQHVNNVKYIGWILESVPTEVFETQELCGLTLEYRR ECGRDSVLESVTAMDPSKEGDRSLYQHLLRLEDGADIAKGRTKWRPKNAGTNGAISTGKTSNGNSIS* SEQ ID NO: 23 Cuphea hyssopifolia (Chs) FATB3b (V204I, C239F, E243D, M251V variant) coding DNA sequence ATGGTGGCTGCCGAAGCAAGTTCTGCACTCTTCTCCGTTCGAACCCCGGGAACCTCCCCTAAACCCGGGA AGTTCGGGAATTGGCCAACGAGCTTGAGCGTCCCCTTCAAGTCCAAATCAAACCACAATGGCGGCTTTCA GGTTAAGGCAAACGCCAGTGCCCGTCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGCCTC GACACTCAGGAGGACACTTCATCGTCGTCCTCTCCTCCTCGGACTTTCATTAACCAGTTGCCCGACTGGA GTATGCTGCTGTCCGCGATCACGACCGTCTTCGTGGCGGCTGAGAAGCAGTGGACGATGCTTGATCGGAA ATCTAAGAGGCCCGACATGCTCATGGACCCGTTTGGGGTTGACAGGGTTGTTCAGGATGGGGCTGTGTTC AGACAGAGTTTTTCGATTAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGA ACATCTTCCAGGAAACATCTCTCAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTAC TCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACAAAAATGCACATCGAGGTTAATCGCTATCCT ACTTGGGGTGATACTATCGAGGTCAATACTTGGGTCTCCGAGTCGGGGAAAACCGGTATGGGTCGTGATT GGCTGATAAGTGATTTTCATACAGGAGACATTCTAATAAGAGCAACGAGCGTGTGTGCTATGATGAATCA AAAGACGAGAAGATTCTCAAAATTTCCATATGAGGTTCGACAGGAGTTAGCGCCTCATTTTGTGGACTCT GCTCCAGTCATTGAAGACTATCAAAAATTGCACAAGCTTGATGTGAAGACGGGTGATTCCATTTGCAATG GCCTAACTCCAAGGTGGAATGACTTGGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGAT TCTCGAGAGTGTTCCAACGGAAGTTTTCGAGACCCAGGAGCTATGTGGCCTCACCCTTGAGTATAGGCGG GAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGGATCCCTCAAAAGAGGGAGACAGATCTC TGTACCAGCACCTTCTTCGGCTTGAGGATGGGGCTGATATCGCGAAGGGCAGAACCAAGTGGCGGCCGAA GAATGCAGGAACCAATGGGGCAATATCAACAGGAAAGACTTCAAATGGAAACTCGATCTCTTAG SEQ ID NO: 24 Cuphea hyssopifolia (Chs) FATB3b (V204I, C239F, E243D, M251V variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGAGGCCTCCTCCGCCCTGTTCTCCGTGCGCACCCCCGGCACCTCCCCCAAGCCCGGCA AGTTCGGCAACTGGCCCACCTCCCTGTCCGTGCCCTTCAAGTCCAAGTCCAACCACAACGGCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCGCCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCCCTG GACACCCAGGAGGACACCTCCTCCTCCTCCTCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGT CCATGCTGCTGTCCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGATGGACCCCTTCGGCGTGGACCGCGTGGTGCAGGACGGCGCCGTGTTC CGCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATGCACATCGAGGTGAACCGCTACCCC ACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGACCGGCATGGGCCGCGACT GGCTGATCTCCGACTTCCACACCGGCGACATCCTGATCCGCGCCACCTCCGTGTGCGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGTTCCCCTACGAGGTGCGCCAGGAGCTGGCCCCCCACTTCGTGGACTCC GCCCCCGTGATCGAGGACTACCAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCTGCAACG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCGTGCCCACCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCCTGACCCTGGAGTACCGCCGC GAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCC TGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCGCCAAGGGCCGCACCAAGTGGCGCCCCAA GAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCATCTCCTGA SEQ ID NO: 25 Cuphea PSR23 (Cu) FATB3 amino acid sequence MVVAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNAGFQVKANASAHPKANGSAVNLKSGSL NTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKCIVRDGLVSR QSFLIRSYEIGADRTASIETLMNHLQETSINHCKSLGLLNDGFGRTPGMCKNDLIWVLTKMQIMVNRYPT WGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSP HVIEDNDQKLHKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRR ECGMDSVLESVTAVDPSENGGRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS* SEQ ID NO: 26 Cuphea PSR23 (Cu) FATB3 coding DNA sequence ATGGTGGTGGCTGCAGCAACTTCTGCATTCTTCCCCGTTCCAGCCCCGGGAACCTCCCCTAAACCCGGGA AGTCCGGCAACTGGCCATCGAGCTTGAGCCCTACCTTCAAGCCCAAGTCAATCCCCAATGCCGGATTTCA GGTTAAGGCAAATGCCAGTGCCCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAAAGTCTGGCAGCCTC AACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCCCGGGCTTTCCTTAACCAGTTGCCTGATTGGAGTA TGCTTCTGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTGGACTATGCTTGATAGGAAATC TAAGAGGCCTGACATGCTCGTGGACTCGGTTGGGTTGAAGTGTATTGTTCGGGATGGGCTCGTGTCCAGA CAGAGTTTTTTGATTAGATCTTATGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGAACC ACTTGCAGGAAACATCTATCAATCATTGTAAGAGTTTGGGTCTTCTCAATGACGGCTTTGGTCGTACTCC TGGGATGTGTAAAAACGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTACCCAACT TGGGGCGATACTGTTGAGATCAATACCTGGTTCTCTCAGTCGGGGAAAATCGGTATGGCTAGCGATTGGC TAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCGTGTGGGCTATGATGAATCAAAA GACGAGAAGATTCTCAAGACTTCCATACGAGGTTCGCCAGGAGTTAACGCCTCATTTTGTGGACTCTCCT CATGTCATTGAAGACAATGATCAGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGCAAGG GTCTAACTCCGAGGTGGAACGACTTGGATGTGAATCAGCACGTAAGCAACGTGAAGTACATTGGGTGGAT TCTCGAGAGTATGCCAATAGAAGTTTTGGAGACACAGGAGCTATGCTCTCTCACCGTAGAATATAGGCGG GAATGCGGAATGGACAGTGTGCTGGAGTCCGTGACTGCTGTGGATCCCTCAGAAAATGGAGGCCGGTCTC AGTACAAGCACCTTCTGCGGCTTGAGGATGGGACTGATATCGTGAAGAGCAGAACTGAGTGGCGACCGAA GAATGCAGGAACTAACGGGGCGATATCAACATCAACAGCAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 27 Cuphea PSR23 (Cu) FATB3 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCbCCAAGCCCGGCA AGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCATCCCCAACGCCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTG AACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCA TGCTGCTGACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTC CAAGCGCCCCGACATGCTGGTGGACTCCGTGGGCCTGAAGTGCATCGTGCGCGACGGCCTGGTGTCCCGC CAGTCCTTCCTGATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCTCCATCAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGGCTTCGGCCGCACCCC CGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCCACC TGGGGCGACACCGTGGAGATCAACACCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGCCTCCGACTGGC TGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAA GACCCGCCGCTTCTCCCGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCC CACGTGATCGAGGACAACGACCAGAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGCAAGG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCGTGGAGTACCGCCGC GAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACCGCCGTGGACCCCTCCGAGAACGGCGGCCGCTCCC AGTACAAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCGTGAAGTCCCGCACCGAGTGGCGCCCCAA GAACGCCGGCACCAACGGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 28 Cuphea wrightii (Cw) FATB3 amino acid sequence MVVAAAASSAFFPVPAPRTTPKPGKFGNWPSSLSPPFKPKSNPNGRFQVKANVSPHPKANGSAVSLKSGS LNTLEDPPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQFTRLDRKSKRPDMLVDWFGSETIVQDGLVF RERFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTSEMCTRDLIWVLTKMQIVVNRYP TWGDTVEINSWFSQSGKIGMGRDWLISDCNTGEILVRATSAWAMMNQKTRRFSKLPCEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYR RECGRESVVESVTSMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNRAIST* SEQ ID NO: 29 Cuphea wrightii (Cw) FATB3 coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTAGAACCACGCCTAAACCCG GGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCGCCCTTCAAGCCCAAGTCAAACCCCAATGGTAGATT TCAGGTTAAGGCAAATGTCAGTCCTCATCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCTGGAGGACCCTCCGTCGTCCCCTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCGGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTTCACTAGGCTCGATCGAAA ATCTAAGAGGCCTGACATGCTAGTGGACTGGTTTGGGTCAGAGACTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGATTTTtGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAGACGCTGATGA ACCACTTGCAGGACACATCTCTGAATCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTCGTAC CTCGGAGATGTGTACAAGAGACCTCATTTGGGTGCTTACAAAAATGCAGATCGTGGTGAATCGCTATCCA ACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCGGGGAAAATCGGTATGGGTCGCGATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTGTAAGAGCAACGAGCGCTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCATGCGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCT CCTCCTGTCATTGAAGACAATGATCGGAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCTACAGAAGTTTTGGAGACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGTGGAAGGGAAAGTGTGGTAGAGTCCGTGACCTCTATGAATCCCTCAAAAGTTGGAGACCGGT CTCAGTACCAACACCTTCTGCGGCTTGAGGATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGAGACC AAAGAATGCAGGAACCAACCGGGCGATATCAACATGA

SEQ ID NO: 30 Cuphea wrightii (Cw) FATB3 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCCGCACCACCCCCAAGCCCG GCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCCCCTTCAAGCCCAAGTCCAACCCCAACGGCCGCTT CCAGGTGAAGGCCAACGTGTCCCCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCTGGAGGACCCCCCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCGCACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTTCACCCGCCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACTGGTTCGGCTCCGAGACCATCGTGCAGGACGGCCTGGTGTTC CGCGAGCGCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CTCCGAGATGTGCACCCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCGTGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCGACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGGTGCGCGCCACCTCCGCCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCTGCGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGAGTCCGTGGTGGAGTCCGTGACCTCCATGAACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACCGCGCCATCTCCACCTGA SEQ ID NO: 31 Cuphea wrightii (Cw) FATB4a amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGSGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGLKSGGF KTQEDSPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGSIVQDGLVFR QNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPT WGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSA PVVEDDDRKLPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRR ECGRESVLESLTAVDPSAEGYASRFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGDFF* SEQ ID NO: 32 Cuphea wrightii (Cw) FATB4a coding DNA sequence TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTGCCATCCGCCGACACCTCCTCCTCGAGACCCG GAAAGCTCGGCAGTGGACCATCGAGCTTGAGCCCCCTCAAGCCCAAATCGATCCCCAATGGCGGCTTGCA GGTTAAGGCAAACGCCAGTG6CCCTCCTAAGATCAATGGTTCCTCGGTCGGTCTAAAGTCGGGCGGTTTC AAGACTCAGGAAGACTCTCCTTCGGCCCCTCCTCCGCGGACTTTTATCAACCAGTTGCCTGATTGGAGTA TGCTTCTTGCTGCAATCACTACTGTCTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACC TAAGAGGCCTGACATGCTCGTGGACCCGTTCGGATTGGGAAGTATTGTTCAGGATGGGCTTGTGTTCAGG CAGAATTTTTCAATTAGGTCCTACGAAATAGGCGCCGATCGAACTGCGTCTATAGAGACGGTGATGAACC ATTTGCAGGAAACAGCTCTCAATCATGTCAAGATTGCTGGGCTTTCTAATGACGGCTTTGGTCGTACTCC TGAGATGTATAAAAGAGACCTTATTTGGGTTGTTGCAAAAATGCAGGTCATGGTTAACCGCTATCCTACT TGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGACTGGC TCATAAGTGATTGCAATACTGGAGAGATTCTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAA GACAAGAAGATTGTCAAAAATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCT CCCGTCGTTGAAGATGATGATCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAGG GTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATCGGATGGAT TCTTGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGCTCCCTGACCCTGGAATACAGGCGG GAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTCGACCCGTCTGCAGAGGGCTATGCGTCCC GGTTTCAGCACCTTCTGCGGCTTGAGGATGGAGGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCCAA GAATGCTGGAATCAATGGGGTGGTACCATCCGAGGAGTCCTCACCTGGAGACTTCTTTTAG SEQ ID NO: 33 Cuphea wrightii (Cw) FATB4a coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCTCCCGCCCCG GCAAGCTGGGCTCCGGCCCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCATCCCCAACGGCGGCCTGCA GGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCGGCTTC AAGACCCAGGAGGACTCCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCA TGCTGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCC CAAGCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGC CAGAACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACC ACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCAACGACGGCTTCGGCCGCACCCC CGAGATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACC TGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGC TGATCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAA GACCCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCC CCCGTGGTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCGCAAGG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCACCCOCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGC GAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGCCGAGGGCTACGCCTCCC GCTTCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAA GAACGCCGGCATCAACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTTCTTCTGA SEQ ID NO: 34 Cuphea wrightii (Cw) FATB4b amino acid sequence MVATAASSAFFPVPSADTSSSRPGKLGNGPSSLSPLKPKSIPNGGLQVKANASAPPKINGSSVGLKSGSF KTQEDAPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGSIVQDGLVFR QNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSSDGFGRTPAMSKRDLIWVVAKMQVMVNRYPA WGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSA PVVEDDDRKLPKLDENTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPAEVLETQELCSLTLEYRR ECGRESVLESLTAVDPSGEGDGSKFQHLLRLEDGGEIVKARTEWRPKNAGINGVVPSEESSPGGDFF* SEQ ID NO: 35 Cuphea wrightii (Cw) FATB4b coding DNA sequence TTGGTGGCTACCGCTGCAAGTTCTGCATTTTTCCCCGTACCATCCGCCGACACCTCCTCATCGAGACCCG GAAAGCTCGGCAATGGGCCATCGAGCTTGAGCCCCCTCAAGCCGAAATCGATCCCCAATGGCGGGTTGCA GGTTAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCTCGGTCGGTCTGAAGTCGGGCAGTTTC AAGACTCAGGAAGACGCTCCTTCGGCCCCTCCTCCTCGGACTTTTATCAACCAGTTGCCTGATTGGAGTA TGCTTCTTGCTGCAATCACTACTGTCTTCTTGGCTGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACC TAAGAGGCCTGACATGCTTGTCGACCCGTTCGGATTGGGAAGTATTGTTCAGGATGGGCTTGTTTTCAGG CAGAATTTCTCGATTAGGTCCTACGAAATAGGCGCTGATCGCACTGCGTCTATAGAGACGGTGATGAACC ATTTGCAGGAAACAGCTCTCAATCATGTTAAGATTGCTGGGCTTTCTAGTGATGGCTTTGGTCGTACTCC TGCGATGTCTAAACGGGACCTCATTTGGGTTGTTGCGAAAATGCAGGTCATGGTTAACCGCTACCCTGCT TGGGGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGACTGGC TCATAAGTGATTGCAACACTGGAGAGATTCTTACAAGAGCATCAAGCGTGTGGGTCATGATGAATCAAAA GACAAGAAGATTGTCAAAAATTCCAGATGAGGTTCGAAATGAGATAGAGCCTCATTTTGTGGACTCTGCG CCCGTCGTTGAAGACGATGACCGGAAACTTCCCAAGCTGGATGAGAACACTGCTGACTCCATCCGCAAGG GTCTAACTCCGAGGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGAT TCTTGAGAGTACTCCAGCAGAAGTTCTGGAGACCCAGGAATTATGTTCCCTGACCCTGGAATACAGGCGG GAATGTGGAAGGGAGAGCGTGCTGGAGTCCCTCACTGCTGTAGATCCGTCTGGAGAGGGCGATGGGTCCA AGTTCCAGCACCTTCTGCGGCTTGAGGATGGAGGTGAGATCGTGAAGGCGAGAACTGAGTGGCGACCAAA GAATGCTGGAATCAATGGGGTGGTACCATCCGAGGAGTCCTCACCTGGTGGAGACTTCTTTTAA SEQ ID NO: 36 Cuphea wrightii (Cw) FATB4b coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCTCCCGCCCCG GCAAGCTGGGCAACGGCCCCTCCTCCCTGTCCCCCCTGAAGCCCAAGTCCATCCCCAACGGCGGCCTGCA GGTGAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCTCCTTC AAGACCCAGGAGGACGCCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCA TGCTGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCC CAAGCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCTCCATCGTGCAGGACGGCCTGGTGTTCCGC CAGAACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACC ACCTGCAGGAGACCGCCCTGAACCACGTGAAGATCGCCGGCCTGTCCTCCGACGGCTTCGGCCGCACCCC CGCCATGTCCAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCGCC TGGGGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGC TGATCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAA GACCCGCCGCCTGTCCAAGATCCCCGACGAGGTGCGCAACGAGATCGAGCCCCACTTCGTGGACTCCGCC CCCGTGGTGGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAACACCGCCGACTCCATCCGCAAGG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCACCCCCGCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGC GAGTGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCGAGGGCGACGGCTCCA AGTTCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGCCCGCACCGAGTGGCGCCCCAA GAACGCCGGCATCAACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGGCGACTTCTTCTGA SEQ ID NO: 37 Cuphea wrightii (Cw) FATB5 amino acid sequence MVAAAASSAFFSVPTPGTPPKPGKFGNWPSSLSVPFKPDNGGFHVKANASAHPKANGSAVNLKSGSLETP PRSFINQLPDLSVLLSKITTVFGAAEKQWKRPGMLVEPFGVDRIFQDGVFFRQSFSIRSYEIGVDRTASI ETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIEVNTWVSESGKNG MGRDWLISDCRTGEILIRATSVWAMMNQNTRRLSKFPYEVRQEIAPHFVDSAPVIEDDQKLQKLDVKTGD SIRDGLTPRWNDLDVNQHVNNVKYIGWILKSVPIEVFETQELCGVTLEYRRECGRDSVLESVTAMDPAKE GDRCVYQHLLRLEDGADITIGRTEWRPKNAGANGAMSSGKTSNGNCLIEGRGWQPFRVVRLIF* SEQ ID NO: 38 Cuphea wrightii (Cw) FATB5 coding DNA sequence ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCTGTTCCAACCCCGGGAACGCCCCCTAAACCCGGGA AGTTCGGTAACTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGACAATGGTGGCTTTCATGTCAAGGC AAACGCCAGTGCCCATCCTAAGGCTAATGGTTCTGCGGTAAATCTAAAGTCTGGCAGCCTCGAGACTCCT CCTCGGAGTTTCATTAACCAGCTGCCGGACTTGAGTGTGCTTCTGTCCAAAATCACGACTGTCTTCGGGG CGGCTGAGAAGCAGTGGAAGAGGCCCGGCATGCTCGTGGAACCGTTTGGGGTTGACAGGATTTTTCAGGA TGGTGTTTtTTTCAGACAGAGTTTTTCTATCAGGTCTTACGAAATAGGCGTTGATCGAACAGCCTCGATA GAGACACTGATGAACATCTTCCAGGAAACATCTTTGAATCATTGCAAGAGTATCGGTCTTCTCAACGATG GCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGACCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGT GAATCGCTATCCTACTTGGGGTGACACTATCGAAGTCAATACTTGGGTCTCGGAGTCGGGGAAAAACGGT ATGGGTCGGGATTGGCTGATAAGTGATTGCCGTACTGGAGAGATTCTTATAAGAGCAACGAGCGTGTGGG CGATGATGAATCAAAACACGAGAAGATTGTCAAAATTTCCATATGAGGTTCGACAGGAGATAGCGCCTCA TTTTGTGGACTCTGCTCCTGTCATTGAAGACGATCAAAAGTTGCAGAAGCTTGATGTGAAGACAGGTGAT TCCATTCGCGATGGTCTAACTCCGAGATGGAATGACTTGGATGTCAATCAACACGTTAACAATGTGAAGT ACATTGGATGGATTCTCAAGAGTGTTCCAATAGAAGTTTTCGAGACACAGGAGCTATGCGGCGTCACACT TGAATATAGGCGGGAATGCGGAAGGGACAGTGTGCTGGAGTCAGTGACCGCTATGGATCCAGCAAAAGAG GGAGACCGGTGTGTGTACCAGCACCTTCTTCGGCTTGAGGATGGAGCTGATATCACTATAGGCAGAACCG AGTGGCGGCCGAAGAATGCAGGAGCCAATGGTGCAATGTCATCAGGAAAGACTTCAAATGGAAACTGTCT CATAGAAGGAAGGGGTTGGCAACCTTTCCGAGTTGTGCGTTTAATTTTCTGA SEQ ID NO: 39 Cuphea wrightii (Cw) FATB5 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCCCCCCCAAGCCCGGCA AGTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGACAACGGCGGCTTCCACGTGAAGGC CAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTGGAGACCCCC CCCCGCTCCTTCATCAACCAGCTGCCCGACCTGTCCGTGCTGCTGTCCAAGATCACCACCGTGTTCGGCG CCGCCGAGAAGCAGTGGAAGCGCCCCGGCATGCTGGTGGAGCCCTTCGGCGTGGACCGCATCTTCCAGGA CGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGTGGACCGCACCGCCTCCATC GAGACCCTGATGAACATCTTCCAGGAGACCTCCCTGAACCACTGCAAGTCCATCGGCCTGCTGAACGACG GCTTCGGCCQCACCCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGT GAACCGCTACCCCACCTGGGGCGACACCATCGAGGTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGGC ATGGGCCGCGACTGGCTGATCTCCGACTGCCGCACCGGUGAGATCCTGATCCGCGCCACCTCCGTGTGGG CCATGATGAACCAGAACACCCGCCGCCTGTCCAAGTTCCCCTACGAGGTGCGCCAGGAGATCGCCCCCCA CTTCGTGGACTCCGCCCCCGTGATCGAGGACGACCAGAAGCTGCAGAAGCTGGACGTGAAGACCGGCGAC TCCATCCGCGACGGCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGT ACATCGGCTGGATCCTGAAGTCCGTGCCCATCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCT GGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCGCCAAGGAG GGCGACCGCTGCGTGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCACCATCGGCCGCACCG AGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGCCATGTCCTCCGGCAAGACCTCCAACGGCAACTGCCT GATCGAGGGCCGCGGCTGGCAGCCCTTCCGCGTGGTGCGCCTGATCTTCTGA SEQ ID NO: 40 Cuphea heterophylla (Cht) FATB1a amino acid sequence MVAAAASSAFFSVPTPGTSTKPGNFGNWPSSLSVPFKPESNHNGGFRVKANASAHPKANGSAVNLKSGSL ETQEDTSSSSPPPRTFIKQLPDWGMLLSKITTVFGAAERQWKRPGMLVEPFGVDRIFQDGVFFRQSFSIR SYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIE VNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDD KKLHKLDVKTGDSIRKGLTPRWNDLDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSV LESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS* SEQ ID NO: 41 Cuphea heterophylla (Cht) FATB1a coding DNA sequence ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCCCGGGAACCTCCACTAAACCCGGGA ACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAAACCACAATGGTGGCTTTCG GGTCAAGGCAAACGCCAGTGCTCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAAAGTCTGGCAGCCTC GAGACTCAGGAGGACACTTCATCGTCGTCCCCTCCTCCTCGGACTTTTATTAAGCAGTTGCCCGACTGGG GTATGCTTCTGTCCAAAATCACGACTGTCTTCGGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCT TGTGGAACCGTTTGGGGTTGACAGGATTTTTCAGGATGGGGTTTTTTTCAGACAGAGTTTTTCGATCAGG TCTTACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAAACATCTC TGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGA CCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCTATCCTACTTGGGGTGATACTATTGAG GTCAATACTTGGGTCTCAGAGTCGGGGAAAAACGGTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTA CCGGAGAAATTCTTATAAGAGCAACGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAA ATTTCCATATGAGGTTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGAT AAAAAATTGCACAAGCTTGATGTTAAGACGGGTGATTCCATTCGCAAGGGTCTAACTCCAAGGTGGAATG ACTTGGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAAGAGTGTTCCAGCAGA AGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTACAGGCGGGAATGTGGAAGGGACAGTGTG CTGGAGTCCGTGACCGCTATGGATACCGCAAAAGAGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGC TTGAGGATGGGGCTGATATCACCATAGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGC AATATCAACAGGAAAGACTTCAAATGAAAACTCTGTCTCTTAG SEQ ID NO: 42 Cuphea heterophylla (Cht) FATB1a coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCCCCGGCACCTCCACCAAGCCCGGCA ACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCAACCACAACGGCGGCTTCCG CGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTG GAGACCCAGGAGGACACCTCCTCCTCCTCCCCCCCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGG GCATGCTGCTGTCCAAGATCACCACCGTGTTCGGCGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCT GGTGGAGCCCTTCGGCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGC TCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGACCTCCC TGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGA CCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAG GTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCGCA CCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCGCAAGACCCGCCGCCTGTCCAA GTTCCCCTACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGAC AAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAAGGGCCTGACCCCCCGCTGGAACG ACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAAGTCCGTGCCCGCCGA GGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTG CTGGAGTCCGTGACCGCCATGGACACCGCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCC TGGAGGACGGCGCCGACATCACCATCGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGC CATCTCCACCGGCAAGACCTCCAACGAGAACTCCGTGTCCTGA SEQ ID NO: 43 Cuphea heterophylla (Cht) FATB1b (P16S, T20P, G94S, G105W, S293F, L305F variant) amino acid sequence MVAAAASSAFFSVPTSGTSPKPGNFGNWPSSLSVPFKPESSHNGGFQVKANASAHPKANGSAVNLKSGSL ETQEDTSSSSPPPRTFIKQLPDWSMLLSKITTVFWAAERQWKRPGMLVEPFGVDRIFQDGVFFRQSFSIR SYEIGADRTASIETLMNIFQETSLNHCKSIGLLNDGFGRTPEMCKRDLIWVVTKIQVEVNRYPTWGDTIE VNTWVSESGKNGMGRDWLISDCRTGEILIRATSVWAMMNRKTRRLSKFPYEVRQEIAPHFVDSAPVIEDD KKLHKLDVKTGDFIRKGLTPRWNDFDVNQHVNNVKYIGWILKSVPAEVFETQELCGVTLEYRRECGRDSV LESVTAMDTAKEGDRSLYQHLLRLEDGADITIGRTEWRPKNAGANGAISTGKTSNENSVS* SEQ ID NO: 44 Cuphea heterophylla (Cht) FATB1b (P16S, T2OP, G94S, G105W, S293F, L305F variant) coding DNA sequence ATGGTGGCTGCCGCAGCAAGTTCTGCATTCTTCTCCGTTCCAACCTCGGGAACCTCCCCTAAACCCGGGA ACTTCGGCAATTGGCCATCGAGCTTGAGCGTCCCCTTCAAGCCCGAATCAAGCCACAATGGTGGCTTTCA GGTCAAGGCAAACGCCAGTGCCCATCCTAAGGCTAACGGTTCTGCAGTAAATCTAAAGTCTGGCAGCCTC GAGACTCAGGAGGACACTTCATCGTCGTCCCCTCCTCCTCGGACTTTTATTAAGCAGTTGCCCGACTGGA GTATGCTTCTGTCCAAAATCACGACTGTCTTCTGGGCGGCTGAGAGGCAGTGGAAGAGGCCCGGCATGCT TGTGGAACCGTTTGGGGTTGACAGGATTTTTCAGGATGGGGTTTTTTTCAGACAGAGTTTTTCGATCAGG TCTTACGAAATAGGCGCTGATCGAACAGCCTCAATAGAGACGCTGATGAACATCTTCCAGGAAACATCTC TGAATCATTGTAAGAGTATCGGTCTTCTCAATGACGGCTTTGGTCGTACTCCTGAGATGTGTAAGAGGGA

CCTCATTTGGGTGGTTACGAAAATTCAGGTCGAGGTGAATCGCTATCCTACTTGGGGTGATACTATTGAG GTCAATACTTGGGTCTCAGAGTCGGGGAAAAACGGTATGGGTCGTGATTGGCTGATAAGCGATTGCCGTA CCGGAGAAATTCTTATAAGAGCAACGAGCGTGTGGGCTATGATGAATCGAAAGACGAGAAGATTGTCAAA ATTTCCATATGAGGTTCGACAGGAGATAGCGCCTCATTTTGTGGACTCTGCTCCTGTCATTGAAGACGAT AAAAAATTGCACAAGCTTGATGTTAAGACGGGTGATTTCATTCGCAAGGGTCTAACTCCAAGGTGGAATG ACTTTGATGTCAATCAGCACGTTAACAATGTGAAGTACATTGGGTGGATTCTCAAGAGTGTTCCAGCAGA AGTTTTCGAGACCCAGGAGCTATGCGGAGTCACCCTTGAGTATAGGCGGGAATGTGGAAGGGACAGTGTG CTGGAGTCCGTGACCGCTATGGATACCGCAAAAGAGGGAGACCGGTCTCTGTACCAGCACCTTCTTCGGC TTGAGGATGGGGCTGATATCACCATAGGCAGAACCGAGTGGCGGCCGAAGAATGCAGGAGCCAATGGGGC AATATCAACAGGAAAGACTTCAAATGAAAACTCTGTCTCTTAG SEQ ID NO: 45 Cuphea heterophylla (Cht) FATB1b (P16S, T20P, G94S, G105W, S293F, L305F variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCGTGCCCACCTCCGGCACCTCCCCCAAGCCCGGCA ACTTCGGCAACTGGCCCTCCTCCCTGTCCGTGCCCTTCAAGCCCGAGTCCTCCCACAACGGCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTG GAGACCCAGGAGGACACCTCCTCCTCCTCCCCCCCCCCCCGCACCTTCATCAAGCAGCTGCCCGACTGGT CCATGCTGCTGTCCAAGATCACCACCGTGTTCTGGGCCGCCGAGCGCCAGTGGAAGCGCCCCGGCATGCT GGTGGAGCCCTTCGGCGTGGACCGCATCTTCCAGGACGGCGTGTTCTTCCGCCAGTCCTTCTCCATCCGC TCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGACCTCCC TGAACCACTGCAAGTCCATCGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGAGATGTGCAAGCGCGA CCTGATCTGGGTGGTGACCAAGATCCAGGTGGAGGTGAACCGCTACCCCACCTGGGGCGACACCATCGAG GTGAACACCTGGGTGTCCGAGTCCGGCAAGAACGGCATGGGCCGCGACTGGCTGATCTCCGACTGCCGCA CCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCGCAAGACCCGCCGCCTGTCCAA GTTCCCCTACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACTCCGCCCCCGTGATCGAGGACGAC AAGAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTTCATCCGCAAGGGCCTGACCCCCCGCTGGAACG ACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCTGAAGTCCGTGCCCGCCGA GGTGTTCGAGACCCAGGAGCTGTGCGGCGTGACCCTGGAGTACCGCCGCGAGTGCGGCCGCGACTCCGTG CTGGAGTCCGTGACCGCCATGGACACCGCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGCTGCGCC TGGAGGACGGCGCCGACATCACCATCGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAACGGCGC CATCTCCACCGGCAAGACCTCCAACGAGAACTCCGTGTCCTGA SEQ ID NO: 46 Cuphea heterophylla (Cht) FATB2b amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTQEGTSSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVDWFGLEIIVQDGLVF RESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYP TWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 47 Cuphea heterophylla (Cht) FATB2b coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCCCTAAACCCG GGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCCGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGGCACTTCGTCGTCCCCTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCGGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA GTCTAAGAAGCCTGACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTTGATGA ACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTATCCA ACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCC CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGATAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGAGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCTATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 48 Cuphea heterophylla (Cht) FATB2b coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGGCACCTCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCGCACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGAAGCCCGACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 49 Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) amino acid sequence MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGSAVSLKSGS LNTKEDTPSSPPPRTFLNQLPDWSRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVDWFGLEIIVQDWLVF RESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYP TWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPLIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 50 Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGTCTAAACCCG GGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTCAAACCCCAATGGTGGATT TCAGGTTAAGGCAAATGCCAGCGCTCATCCTAAGGCTAACGGGTCTGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTAAGGAGGACACTCCGTCGTCCCCTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCGGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA GTCTAAGAAGCCTGACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATTGGCTCGTGTTC AGAGAGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTTGATGA ACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTLGTAC CCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTATCCA ACTTGCCGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCTCCTCATTTTGTGGACGCC CCTCCTCTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGAGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 51 Cuphea heterophylla (Cht) FATB2a (S17P, P21S, T28N, L30P, S33L, G76D, S78P, G137W variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCACCTCCAAGCCCG GCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTCCAACCCCAACGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCAAGGAGGACACCCCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCGCACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGAAGCCCGACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACTGGCTGGTGTTC CGCGAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCCTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 52 Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTKEDTPSSPPPRTFLNQLPDWNRLRTAITTVFVAAEKQLTMLDRKSKKPDMHVDWFGLEIIVQDGLVF RESFSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYP TWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 53 Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCCCTAAACCCG GGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCCGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTAAGGAGGACACTCCGTCGTCCCCTCCTCCTCGGACTTTCCTTAACCAGTTGCCTGATTGGA ATAGGCTTCGGACTGCAATCACGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA GTCTAAGAAGCCTGACATGCACGTGGACTGGTTTGGGTTGGAGATTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTTTTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTTGATGA ACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTATCCA ACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCC CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGGGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCTATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 54 Cuphea heterophylla (Cht) FATB2c (G76D, S78P variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCAAGGAGGACACCCCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGA ACCGCCTGCGCACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGAAGCCCGACATGCACGTGGACTGGTTCGGCCTGGAGATCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 55 Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) amino acid sequence MVVAAAASSAFFPVPAPGTTSKPGKFGNWPSSLSPSFKPKSNPNGGFQVKANASAHPKANGSAVSLKSGS LNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVDLFGLESIVQDGLVF RESYSIRSYEIGADRTASIETLMNHLQDTSLNHCKSVGLLNDGFGRTPEMCKRDLIWVLTKMQIMVNRYP TWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYR RECGRESVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 56 Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGTTCTGCATTCTTCCCTGTTCCAGCACCTGGAACCACGTCTAAACCCG GGAAGTTCGGCAATTGGCCATCGAGCTTGAGCCCTTCCTTCAAGCCCAAGTCAAACCCCAATGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCTGCGGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCTGACTGCAATCTCGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA ATCTAAGAGGCCTGACATGCTCGTGGACTTGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTATTCGATCAGGTCTTACGAAATAGGCGCTGATCGAACAGCCTCTATAGAAACGTTGATGA ACCATTTGCAGGACACATCTTTGAACCATTGTAAGAGTGTGGGTCTTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGTAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCATGGTGAATCGCTATCCA ACTTGGGGCGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAATACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCT CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGCA AGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGAAAGTGTGCTGGAGTCCGTGACCGCTATGAATCCCTCAAAAGTTGGAGACCGGT CTCAGTACCAGCACCTTCTACGGCTTGAGGATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 57 Cuphea heterophylla (Cht) FATB2d (S21P, T28N, L30P, S33L, G76D, R97L, H124L, W127L, I132S, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCACCTCCAAGCCCG GCAAGTTCGGCAACTGGCCCTCCTCCCTGTCCCCCTCCTTCAAGCCCAAGTCCAACCCCAACGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCTGACCGCCATCTCCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACCTGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTACTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGA ACCACCTGCAGGACACCTCCCTGAACCACTGCAAGTCCGTGGGCCTGCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCC ACCTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACtGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAACACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGCA AGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGAGTCCGTGCTGGAGTCCGTGACCGCCATGAACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

SEQ ID NO: 58 Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTQEDTSSSPPPQTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVDWFGLESIVQDGLVF RESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYP NWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQNTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSIRKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLTLEYR RECGRDSVLESVTAMNPSKVGDRSQYQHLLRLEDGADIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 59 Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCCCTAAACCCG GGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCTCAGACATTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCTGACAGCAATCTCGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA ATCTAAAAGGCCTGACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGGTGATGA ACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCTTGGTGAATCGCTATCCA AATTGGGGTGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAATACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTTGACGCT CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTCGCA AGGGTCTAACTCCGGGGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAACAGAAGTTTTGGAGACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCCGTGACCGCTATGAATCCCTCAAAAGTTGGAGACCGGT CTCAGTACCAGCACCTTCTACGGCTTGAGGATGGGGCTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 60 Cuphea heterophylla (Cht) FATB2e (G76D, R97L, H124L, I132S, G152S, H165L, T211N, K258N, C303R, E309G, K334T, T386A variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCAGACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCTGACCGCCATCTCCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGTGATGA ACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCCTGGTGAACCGCTACCCC AACTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAACACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCCGCA AGGGCCTGACCCCCGGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCACCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGAACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCGCCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 61 Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) amino acid sequence MVVAAAASSAFFPVPASGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTQEGTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVDWFGLESIVQDGLVF RESYSIRSYEISADRTASIETVMNLLQETSLNHCKSMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYP NWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNGNSVS* SEQ ID NO: 62 Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) coding DNA sequence ATGGTGGTGGCTGCTGCAGCAAGCTCTGCATTCTTCCCTGTTCCGGCATCTGGAACCTCCCCTAAACCCG GGAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAAGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCCGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGGCACTTCGTCGTCCCCTCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCTGACTGCAATCTCGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA ATCTAAGAGGCCTGACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTC AGAGAGAGTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAGCCTCTATAGAGACGGTGATGA ACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCTTGGTGAATCGCTATCCA AATTGGGGTGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAATGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCC CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAACGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGAGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGGAAACTCGGTCTCTTAG SEQ ID NO: 63 Cuphea heterophylla (Cht) FATB2f (R97L, H124L, I132S, G152S, H165L, T211N variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCGCCTCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGGCACCTCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCTGACCGCCATCTCCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTC CGCGAGTCCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCGCCTCCATCGAGACCGTGATGA ACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCCTGGTGAACCGCTACCCC AACTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGAGTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 64 Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S1431, G152S, A157T, H165L, T211N, G414A variant) amino acid sequence MVVAATASSAFFPVPVPGTSPKPGKFGTWLSSSSPSYKPKSNPSGGFQVKANASAHPKANGSAVSLKSGS LNTQEDTSSSPPPRTFLNQLPDWSRLLTAISTVFVAAEKQLTMLDRKSKRPDMLVDWFGLESIVQDGLVF REIYSIRSYEISADRTTSIETVMNLLQETSLNHCKSMGILNDGFGRTPEMCKRDLIWVLTKMQILVNRYP NWGDTVEINSWFSQSGKIGMGRNWLISDCNTGEILIRATSIWAMMNQKTRRFSKLPNEVRQEIAPHFVDA PPVIEDNDRKLHKFDVKTGDSICKGLTPEWNDLDVNQHVSNVKYIGWILESMPKEVLDTQELCSLTLEYR RECGRDSVLESVTAMDPSKVGDRSQYQHLLRLEDGTDIMKGRTEWRPKNAGTNGAISTGKTSNANSVS* SEQ ID NO: 65 Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S1431, G152S, A157T, H165L, T211N, G414A variant) coding DNA sequence ATGGTGGTGGCTGCTACAGCAAGTTCTGCATTCTTCCCTGTTCCTGTACCTGGAACCTCCCCTAAACCCG GAAAGTTCGGGACTTGGCTATCGAGCTCGAGCCCTTCCTACAAGCCCAAGTCAAACCCCAGTGGTGGATT TCAGGTTAAGGCAAATGCCAGTGCTCATCCTAAGGCTAACGGTTCTGCAGTAAGTCTAAAGTCTGGCAGC CTCAACACTCAGGAGGACACTTCGTCGTCCCCTCCTCCTCGGACATTCCTTAACCAGTTGCCTGATTGGA GTAGGCTTCTGACTGCAATCTCGACCGTCTTCGTGGCGGCAGAGAAGCAGTTGACTATGCTCGATCGAAA ATCTAAGAGGCCTGACATGCTCGTGGACTGGTTTGGGTTGGAGAGTATTGTTCAGGATGGGCTCGTGTTC AGAGAGATTTATTCGATCAGGTCTTACGAAATAAGCGCTGATCGAACAACCTCTATAGAGACGGTGATGA ACCTCTTGCAGGAAACATCTCTCAATCATTGTAAGAGTATGGGTATTCTCAATGACGGCTTTGGTCGTAC CCCGGAGATGTGCAAAAGGGACCTCATTTGGGTGCTTACAAAAATGCAGATCTTGGTGAATCGCTATCCA AATTGGGGTGATACTGTCGAGATCAATAGCTGGTTCTCCCAGTCCGGGAAAATCGGTATGGGTCGCAATT GGCTAATAAGTGATTGCAACACAGGAGAAATTCTTATAAGAGCAACGAGCATTTGGGCCATGATGAATCA AAAGACGAGAAGATTCTCAAAACTTCCAAACGAGGTTCGCCAGGAGATAGCGCCTCATTTTGTGGACGCC CCTCCTGTCATTGAAGACAATGATCGAAAATTGCATAAGTTTGATGTGAAGACTGGTGATTCCATTTGCA AGGGTCTAACACCGGAGTGGAATGACTTGGATGTCAATCAGCACGTAAGCAACGTGAAGTACATTGGGTG GATTCTCGAGAGTATGCCAAAAGAAGTTTTGGACACCCAGGAGCTATGCTCTCTCACCCTTGAATATAGG CGGGAATGCGGAAGGGACAGTGTGCTGGAGTCTGTGACCGCTATGGATCCCTCAAAAGTTGGAGACCGAT CTCAGTACCAGCACCTTCTGCGGCTTGAAGATGGGACTGATATCATGAAGGGCAGAACTGAGTGGCGACC AAAGAATGCAGGAACCAACGGGGCGATATCAACAGGAAAGACTTCAAATGCAAACTCGGTCTCTTAG SEQ ID NO: 66 Cuphea heterophylla (Cht) FATB2g (A6T, A16V, S17P, G76D, R97L, H124L, I132S, S1431, G152S, A157T, H165L, T211N, G414A variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGTGGCCGCCACCGCCTCCTCCGCCTTCTTCCCCGTGCCCGTGCCCGGCACCTCCCCCAAGCCCG GCAAGTTCGGCACCTGGCTGTCCTCCTCCTCCCCCTCCTACAAGCCCAAGTCCAACCCCTCCGGCGGCTT CCAGGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGTCCGGCTCC CTGAACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCACCTTCCTGAACCAGCTGCCCGACTGGT CCCGCCTGCTGACCGCCATCTCCACCGTGTTCGTGGCCGCCGAGAAGCAGCTGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACATGCTGGTGGACTGGTTCGGCCTGGAGTCCATCGTGCAGGACGGCCTGGTGTTC CGCGAGATCTACTCCATCCGCTCCTACGAGATCTCCGCCGACCGCACCACCTCCATCGAGACCGTGATGA ACCTGCTGCAGGAGACCTCCCTGAACCACTGCAAGTCCATGGGCATCCTGAACGACGGCTTCGGCCGCAC CCCCGAGATGTGCAAGCGCGACCTGATCTGGGTGCTGACCAAGATGCAGATCCTGGTGAACCGCTACCCC AACTGGGGCGACACCGTGGAGATCAACTCCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGGCCGCAACT GGCTGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCATCTGGGCCATGATGAACCA GAAGACCCGCCGCTTCTCCAAGCTGCCCAACGAGGTGCGCCAGGAGATCGCCCCCCACTTCGTGGACGCC CCCCCCGTGATCGAGGACAACGACCGCAAGCTGCACAAGTTCGACGTGAAGACCGGCGACTCCATCTGCA AGGGCCTGACCCCCGAGTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTG GATCCTGGAGTCCATGCCCAAGGAGGTGCTGGACACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGC CGCGACTGCGGCCGCGACTCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGTGGGCGACCGCT CCCAGTACCAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCATGAAGGGCCGCACCGAGTGGCGCCC CAAGAACGCCGGCACCAACGGCGCCATCTCCACCGGCAAGACCTCCAACGCCAACTCCGTGTCCTGA SEQ ID NO: 67 Cuphea heterophylla (Cht) FATB3aamino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSLKSCSLK THEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGLGRIVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLNEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAP VIEDDDWKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRE CGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS* SEQ ID NO: 68 Cuphea heterophylla (Cht) FATB3a coding DNA sequence ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCGTCCCCGGACACCTCCTCTAGACCGGGAA AGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTGCCAATGCTGGGCTGCAGGT TAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCTCGGTCAGTCTAAAGTCTTGCAGTCTCAAG ACTCATGAAGACACTCCTTCAGCTCCTCCTCCGCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGC TTCTTGCTGCAATCACTACTGTCTTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAA GAGGCCTGACATGCTTGTGGACCCGTTCGGATTGGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAG AATTTTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCCATAGAGACGGTGATGAACCACT TGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTAATGAAGGCTTTGGTCGTACTCCTGA GATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGTCATGGTTAACCGCTATCCTACTTGG GGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGATTGGCTCA TAAGTGATTGCAATACAGGAGAAATTCTTACAAGGGCATCAAGTGTGTGGGTCATGATGAATCAAAAGAC AAGAAAATTGTCAAAGATTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCC GTCATTGAAGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGGGTC TAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGATTCT TGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTACCCTGGAATACAGGCGGGAA TGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGTGGACCCCTCTGGAAAGGGCTTTGGGCCCCAGT TTCAGCACCTTCTGAGGCTTGAGGATGGAGGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGAC TGCAGGTATCAATGCCACGATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG SEQ ID NO: 69 Cuphea heterophylla (Cht) FATB3a coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCCAACGCCGGCCTGCAGGT GAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGTCCCTGAAGTCCTGCTCCCTGAAG ACCCACGAGGACACCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAA GCGCCCCGACATGCTGGTGGACCCCTTCGGCCTGGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGAACGAGGGCTTCGGCCGCACCCCCGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGAC CCGCAAGCTGTCCAAGATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCC GTGATCGAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCC TGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCT GGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAG TGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCCAGT TCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAC CGCCGGCATCAACGGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA SEQ ID NO: 70 Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N1791 variant) amino acid sequence MVATAASSAFFPVPSPDTSSRPGKLGNGSSSLRPLKPKFVANAGLQVKANASAPPKINGSSVSLKSGSLK TQEDTPSAPPPRTFINQLPDWSMLLAAITTVFLAAEKQWMMLDWKPKRPDMLVDPFGFGRIVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKSAGLLIEGFGRTPEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRHEIEPHFVDSAP VIEDDDWKLPKLDEKTADSIRKGLTPKWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRE CGRESVLESLTAVDPSGKGFGPQFQHLLRLEDGGEIVKGRTEWRPKTAGINGTIASGETSPGNS* SEQ ID NO: 71 Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) coding DNA sequence ATGGTGGCCACCGCTGCAAGTTCTGCATTCTTCCCGGTGCCATCCCCGGACACCTCCTCTAGACCGGGAA AGCTCGGAAATGGGTCATCAAGCTTGAGGCCCCTCAAGCCCAAATTTGTTGCCAATGCTGGGCTGCAGGT TAAGGCAAACGCCAGTGCCCCTCCTAAGATCAATGGTTCCTCGGTCAGTCTAAAGTCTGGCAGTCTCAAG ACTCAGGAAGACACTCCTTCGGCTCCTCCTCCGCGGACTTTTATCAACCAGTTGCCTGATTGGAGCATGC TTCTTGCTGCAATCACTACTGTCTTCTTGGCAGCAGAGAAGCAGTGGATGATGCTTGATTGGAAACCAAA GAGGCCTGACATGCTTGTGGACCCGTTCGGATTTGGAAGGATTGTTCAGGATGGGCTTGTGTTCAGGCAG AATTTTTCGATTAGGTCCTATGAAATAGGCGCTGATCGCACTGCATCTATAGAGACGGTGATGAACCACT TGCAGGAAACGGCTCTCAATCATGTTAAGAGTGCGGGGCTTCTTATTGAAGGCTTTGGTCGTACTCCTGA GATGTATAAAAGGGACCTTATTTGGGTTGTCGCGAAAATGCAGGTCATGGTTAACCGCTATCCTACTTGG GGTGACACGGTTGAAGTGAATACTTGGGTTGCCAAGTCAGGGAAAAATGGTATGCGTCGTGATTGGCTCA TAAGTGATTGCAATACAGGAGAAATTCTTACTAGAGCATCAAGTGTGTGGGTCATGATGAATCAAAAGAC AAGAAAATTGTCAAAGATTCCAGATGAGGTTCGGCATGAGATAGAGCCTCATTTTGTGGACTCTGCTCCC GTCATTGAAGACGATGACTGGAAACTTCCCAAGCTGGATGAGAAAACTGCTGACTCCATCCGCAAGGGTC TAACTCCGAAGTGGAATGACTTGGATGTCAATCAGCACGTCAACAACGTGAAGTACATTGGGTGGATTCT TGAGAGTACTCCACCAGAAGTTCTGGAGACCCAGGAGTTATGTTCCCTTACCCTGGAATACAGGCGGGAA TGCGGAAGGGAGAGTGTGCTGGAGTCCCTCACTGCTGTGGACCCCTCTGGAAAGGGCTTTGGGCCCCAGT

TTCAGCACCTTCTGAGGCTTGAGGATGGAGGTGAGATCGTAAAGGGGAGAACTGAGTGGCGACCCAAGAC TGCAGGTATCAATGGGACGATTGCATCTGGGGAGACCTCACCTGGAAACTCTTAG SEQ ID NO: 72 Cuphea heterophylla (Cht) FATB3b (C67G, H72Q, L128F, N179I variant) coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCCCCGACACCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCTCCTCCTCCCTGCGCCCCCTGAAGCCCAAGTTCGTGGCCAACGCCGGCCTGCAGGT GAAGGCCAACGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGTCCCTGAAGTCCGGCTCCCTGAAG ACCCAGGAGGACACCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGTGTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACTGGAAGCCCAA GCGCCCCGACATGCTGGTGGACCCCTTCGGCTTCGGCCGCATCGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTGAAGTCCGCCGGCCTGCTGATCGAGGGCTTCGGCCGCACCCCCGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGAC CCGCAAGCTGTCCAAGATCCCCGACGAGGTGCGCCACGAGATCGAGCCCCACTTCGTGGACTCCGCCCCC GTGATCGAGGACGACGACTGGAAGCTGCCCAAGCTGGACGAGAAGACCGCCGACTCCATCCGCAAGGGCC TGACCCCCAAGTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGGCTGGATCCT GGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAG TGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCAAGGGCTTCGGCCCCCAGT TCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAC CGCCGGCATCAACGGCACCATCGCCTCCGGCGAGACCTCCCCCGGCAACTCCTGA SEQ ID NO: 73 Cuphea viscosissima (Cvis) FATB1 amino acid sequence MVAAAATSAFFPVPAPGTSPKPGKSGNWPSSLSPTFKPKSIPNGGFQVKANASAHPKANGSAVNLKSGSL NTQEDTSSSPPPRAFLNQLPDWSMLLTAITTVFVAAEKQWTMLDRKSKRPDMLVDSVGLKSIVRDGLVSR HSFSIRSYEIGADRTASIETLMNHLQETTINHCKSLGLHNDGFGRTPGMCKNDLIWVLTKMQIMVNRYPT WGDTVEINTWFSQSGKIGMASDWLISDCNTGEILIRATSVWAMMNQKTRRFSRLPYEVRQELTPHFVDSP HVIEDNDQKLRKFDVKTGDSIRKGLTPRWNDLDVNQHVSNVKYIGWILESMPIEVLETQELCSLTVEYRR ECGMDSVLESVTAVDPSENGGRSQYKHLLRLEDGTDIVKSRTEWRPKNAGTNGAISTSTAKTSNGNSVS SEQ ID NO: 74 Cuphea viscosissima (Cvis) FATB1 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGG6CGCCGCCGCCACCTCCGCCTTCTTCCCCGTGCCCGCCCCCGGCACCTCCCCCAAGCCCGGCA AGTCCGGCAACTGGCCCTCCTCCCTGTCCCCCACCTTCAAGCCCAAGTCCATCCCCAACGGCGGCTTCCA GGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGAACCTGAAGTCCGGCTCCCTG AACACCCAGGAGGACACCTCCTCCTCCCCCCCCCCCCGCGCCTTCCTGAACCAGCTGCCCGACTGGTCCA TGCTGCTGACCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAAGTC CAAGCGCCCCGACATGCTGGTGGACTCCGTGGGCCTGAAGTCCATCGTGCGCGACGGCCTGGTGTCCCGC CACTCCTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCCTGATGAACC ACCTGCAGGAGACCACCATCAACCACTGCAAGTCCCTGGGCCTGCACAACGACGGCTTCGGCCGCACCCC CGGCATGTGCAAGAACGACCTGATCTGGGTGCTGACCAAGATGCAGATCATGGTGAACCGCTACCCCACC TGGGGCGACACCGTGGAGATCAACACCTGGTTCTCCCAGTCCGGCAAGATCGGCATGGCCTCCGACTGGC TGATCTCCGACTGCAACACCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAA GACCCGCCGCTTCTCCCGCCTGCCCTACGAGGTGCGCCAGGAGCTGACCCCCCACTTCGTGGACTCCCCC CACGTGATCGAGGACAACGACCAGAAGCTGCGCAAGTTCGACGTGAAGACCGGCGACTCCATCCGCAAGG GCCTGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGTCCAACGTGAAGTACATCGGCTGGAT CCTGGAGTCCATGCCCATCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCGTGGAGTACCGCCGC GAGTGCGGCATGGACTCCGTGCTGGAGTCCGTGACCGCCGTGGACCCCTCCGAGAACGGCGGCCGCTCCC AGTACAAGCACCTGCTGCGCCTGGAGGACGGCACCGACATCGTGAAGTCCCGCACCGAGTGGCGCCCCAA GAACGCCGGCACCAACGGCGCCATCTCCACCTCCACCGCCAAGACCTCCAACGGCAACTCCGTGTCCTGA SEQ ID NO: 75 Cuphea viscosissima (Cvis) FATB2 amino acid sequence MVATAASSAFFPVPSADTSSRPGKLGNGPSSFSPLKPKSIPNGGLQVKASASAPPKINGSSVGLKSGGLK THDDAPSAPPPRTFINQLPDWSMLLAAITTAFLAAEKQWMMLDRKPKRLDMLEDPFGLGRVVQDGLVFRQ NFSIRSYEIGADRTASIETVMNHLQETALNHVKTAGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTW GDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRASSVWVMMNQKTRKLSKIPDEVRREIEPHFVDSAP VIEDDDRKLPKLDEKSADSIRKGLTPRWNDLDVNQHVNNAKYIGWILESTPPEVLETQELCSLTLEYRRE CGRESVLESLTAVDPSGEGYGSQFQHLLRLEDGGEIVKGRTEWRPKNAGINGVVPSEESSPGDYS SEQ ID NO: 76 Cuphea viscosissima (Cvis) FATB2 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCACCGCCGCCTCCTCCGCCTTCTTCCCCGTGCCCTCCGCCGACACCTCCTCCCGCCCCGGCA AGCTGGGCAACGGCCCCTCCTCCTTCTCCCCCCTGAAGCCCAAGTCCATCCCCAACGGCGGCCTGCAGGT GAAGGCCTCCGCCTCCGCCCCCCCCAAGATCAACGGCTCCTCCGTGGGCCTGAAGTCCGGCGGCCTGAAG ACCCACGACGACGCCCCCTCCGCCCCCCCCCCCCGCACCTTCATCAACCAGCTGCCCGACTGGTCCATGC TGCTGGCCGCCATCACCACCGCCTTCCTGGCCGCCGAGAAGCAGTGGATGATGCTGGACCGCAAGCCCAA GCGCCTGGACATGCTGGAGGACCCCTTCGGCCTGGGCCGCGTGGTGCAGGACGGCCTGGTGTTCCGCCAG AACTTCTCCATCCGCTCCTACGAGATCGGCGCCGACCGCACCGCCTCCATCGAGACCGTGATGAACCACC TGCAGGAGACCGCCCTGAACCACGTAAGACCGCCGGCCTGTCCAACGACGGCTTCGGCCGCACCCCCGA GATGTACAAGCGCGACCTGATCTGGGTGGTGGCCAAGATGCAGGTGATGGTGAACCGCTACCCCACCTGG GGCGACACCGTGGAGGTGAACACCTGGGTGGCCAAGTCCGGCAAGAACGGCATGCGCCGCGACTGGCTGA TCTCCGACTGCAACACCGGCGAGATCCTGACCCGCGCCTCCTCCGTGTGGGTGATGATGAACCAGAAGAC CCGCAAGCTGTCCAAGATCCCCGACGAGGTGCGCCGCGAGATCGAGCCCCACTTCGTGGACTCCGCCCCC GTGATCGAGGACGACGACCGCAAGCTGCCCAAGCTGGACGAGAAGTCCGCCGACTCCATCCGCAAGGGCC TGACCCCCCGCTGGAACGACCTGGACGTGAACCAGCACGTGAACAACGCCAAGTACATCGGCTGGATCCT GGAGTCCACCCCCCCCGAGGTGCTGGAGACCCAGGAGCTGTGCTCCCTGACCCTGGAGTACCGCCGCGAG TGCGGCCGCGAGTCCGTGCTGGAGTCCCTGACCGCCGTGGACCCCTCCGGCGAGGGCTACGGCTCCCAGT TCCAGCACCTGCTGCGCCTGGAGGACGGCGGCGAGATCGTGAAGGGCCGCACCGAGTGGCGCCCCAAGAA CGCCGGCATCAACGGCGTGGTGCCCTCCGAGGAGTCCTCCCCCGGCGACTACTCCTGA SEQ ID NO: 77 Cuphea viscosissima (Cvis) FATB3 amino acid sequence MVAAAASSAFFSFPTPGTSPKPGKFGNWPSSLSIPFNPKSNHNGGIQVKANASAHPKANGSAVSLKAGSL ETQEDTSSPSPPPRTFISQLPDWSMLVSAITTVFVAAEKQWTMLDRKSKRPDVLVEPFVQDGVSFRQSFS IRSYEIGVDRTASIETLMNIFQETSLNHCKSLGLLNDGFGRTPEMCKRDLIWVVTKMQIEVNRYPTWGDT IEVTTWVSESGKNGMSRDWLISDCHSGEILIRATSVWAMMNQKTRRLSKIPDEVRQEIVPYFVDSAPVIE DDRKLHKLDVKTGDSIRNGLTPRWNDFDVNQHVNNVKYIAWLLKSVPTEVFETQELCGLTLEYRRECRRD SVLESVTAMDPSKEGDRSLYQHLLRLENGADIALGRTEWRPKNAGATGAVSTGKTSNGNSVS SEQ ID NO: 78 Cuphea viscosissima (Cvis) FATB3 coding DNA sequence codon optimized for Prototheca moriformis ATGGTGGCCGCCGCCGCCTCCTCCGCCTTCTTCTCCTTCCCCACCCCCGGCACCTCCCCCAAGCCCGGCA AGTTCGGCAACTGGCCCTCCTCCCTGTCCATCCCCTTCAACCCCAAGTCCAACCACAACGGCGGCATCCA GGTGAAGGCCAACGCCTCCGCCCACCCCAAGGCCAACGGCTCCGCCGTGTCCCTGAAGGCCGGCTCCCTG GAGACCCAGGAGGACACCTCCTCCCCCTCCCCCCCCCCCCGCACCTTCATCTCCCAGCTGCCCGACTGGT CCATGCTGGTGTCCGCCATCACCACCGTGTTCGTGGCCGCCGAGAAGCAGTGGACCATGCTGGACCGCAA GTCCAAGCGCCCCGACGTGCTGGTGGAGCCCTTCGTGCAGGACGGCGTGTCCTTCCGCCAGTCCTTCTCC ATCCGCTCCTACGAGATCGGCGTGGACCGCACCGCCTCCATCGAGACCCTGATGAACATCTTCCAGGAGA CCTCCCTGAACCACTGCAAGTCCCTGGGCCTGCTGAACGACGGCTTCGGCCGCACCCCCGAGATGTGCAA GCGCGACCTGATCTGGGTGGTGACCAAGATGCAGATCGAGGTGAACCGCTACCCCACCTGGGGCGACACC ATCGAGGTGACCACCTGGGTGTCCGAGTCCGGCAAGAACGGCATGTCCCGCGACTGGCTGATCTCCGACT GCCACTCCGGCGAGATCCTGATCCGCGCCACCTCCGTGTGGGCCATGATGAACCAGAAGACCCGCCGCCT GTCCAAGATCCCCGACGAGGTGCGCCAGGAGATCGTGCCCTACTTCGTGGACTCCGCCCCCGTGATCGAG GACGACCGCAAGCTGCACAAGCTGGACGTGAAGACCGGCGACTCCATCCGCAACGGCCTGACCCCCCGCT GGAACGACTTCGACGTGAACCAGCACGTGAACAACGTGAAGTACATCGCCTGGCTGCTGAAGTCCGTGCC CACCGAGGTGTTCGAGACCCAGGAGCTGTGCGGCCTGACCCTGGAGTACCGCCGCGAGTGCCGCCGCGAC TCCGTGCTGGAGTCCGTGACCGCCATGGACCCCTCCAAGGAGGGCGACCGCTCCCTGTACCAGCACCTGC TGCGCCTGGAGAACGGCGCCGACATCGCCCTGGGCCGCACCGAGTGGCGCCCCAAGAACGCCGGCGCCAC CGGCGCCGTGTCCACCGGCAAGACCTCCAACGGCAACTCCGTGTCCTGA

Sequence CWU 1

1

1151380PRTCinnamomum camphora 1Met Ala Thr Thr Ser Leu Ala Ser Ala Phe Cys Ser Met Lys Ala Val 1 5 10 15 Met Leu Ala Arg Asp Gly Arg Gly Leu Lys Pro Arg Ser Ser Asp Leu 20 25 30 Gln Leu Arg Ala Gly Asn Ala Gln Thr Ser Leu Lys Met Ile Asn Gly 35 40 45 Thr Lys Phe Ser Tyr Thr Glu Ser Leu Lys Lys Leu Pro Asp Trp Ser 50 55 60 Met Leu Phe Ala Val Ile Thr Thr Ile Phe Ser Ala Ala Glu Lys Gln 65 70 75 80 Trp Thr Asn Leu Glu Trp Lys Pro Lys Pro Asn Pro Pro Gln Leu Leu 85 90 95 Asp Asp His Phe Gly Pro His Gly Leu Val Phe Arg Arg Thr Phe Ala 100 105 110 Ile Arg Ser Tyr Glu Val Gly Pro Asp Arg Ser Thr Ser Ile Val Ala 115 120 125 Val Met Asn His Leu Gln Glu Ala Ala Leu Asn His Ala Lys Ser Val 130 135 140 Gly Ile Leu Gly Asp Gly Phe Gly Thr Thr Leu Glu Met Ser Lys Arg 145 150 155 160 Asp Leu Ile Trp Val Val Lys Arg Thr His Val Ala Val Glu Arg Tyr 165 170 175 Pro Ala Trp Gly Asp Thr Val Glu Val Glu Cys Trp Val Gly Ala Ser 180 185 190 Gly Asn Asn Gly Arg Arg His Asp Phe Leu Val Arg Asp Cys Lys Thr 195 200 205 Gly Glu Ile Leu Thr Arg Cys Thr Ser Leu Ser Val Met Met Asn Thr 210 215 220 Arg Thr Arg Arg Leu Ser Lys Ile Pro Glu Glu Val Arg Gly Glu Ile 225 230 235 240 Gly Pro Ala Phe Ile Asp Asn Val Ala Val Lys Asp Glu Glu Ile Lys 245 250 255 Lys Pro Gln Lys Leu Asn Asp Ser Thr Ala Asp Tyr Ile Gln Gly Gly 260 265 270 Leu Thr Pro Arg Trp Asn Asp Leu Asp Ile Asn Gln His Val Asn Asn 275 280 285 Ile Lys Tyr Val Asp Trp Ile Leu Glu Thr Val Pro Asp Ser Ile Phe 290 295 300 Glu Ser His His Ile Ser Ser Phe Thr Ile Glu Tyr Arg Arg Glu Cys 305 310 315 320 Thr Arg Asp Ser Val Leu Gln Ser Leu Thr Thr Val Ser Gly Gly Ser 325 330 335 Ser Glu Ala Gly Leu Val Cys Glu His Leu Leu Gln Leu Glu Gly Gly 340 345 350 Ser Glu Val Leu Arg Ala Lys Thr Glu Trp Arg Pro Lys Leu Ser Phe 355 360 365 Arg Gly Ile Ser Val Ile Pro Ala Glu Ser Ser Val 370 375 380 21128DNACinnamomum camphora 2ttagcttctg ctttctgctc gatgaaagct gtaatgttgg ctcgtgatgg caggggcttg 60aaacccagga gcagtgattt gcagctgagg gcgggaaatg cacaaacctc tttgaagatg 120atcaatggga ccaagttcag ttacacagag agcttgaaaa agttgcctga ctggagcatg 180ctctttgcag tgatcacgac catcttttcg gctgctgaga agcagtggac caatctagag 240tggaagccga agccgaatcc accccagttg cttgatgacc attttgggcc gcatgggtta 300gttttcaggc gcacctttgc catcagatcg tatgaggtgg gacctgaccg ctccacatct 360atagtggctg ttatgaatca cttgcaggag gctgcactta atcatgcgaa gagtgtggga 420attctaggag atggattcgg tacgacgcta gagatgagta agagagatct gatatgggtt 480gtgaaacgca cgcatgttgc tgtggaacgg taccctgctt ggggtgatac tgttgaagta 540gagtgctggg ttggtgcatc gggaaataat ggcaggcgcc atgatttcct tgtccgggac 600tgcaaaacag gcgaaattct tacaagatgt accagtcttt cggtgatgat gaatacaagg 660acaaggaggt tgtccaaaat ccctgaagaa gttagagggg agatagggcc tgcattcatt 720gataatgtgg ctgtcaagga cgaggaaatt aagaaaccac agaagctcaa tgacagcact 780gcagattaca tccaaggagg attgactcct cgatggaatg atttggatat caatcagcac 840gttaacaaca tcaaatacgt tgactggatt cttgagactg tcccagactc aatctttgag 900agtcatcata tttccagctt cactattgaa tacaggagag agtgcacgag ggatagcgtg 960ctgcagtccc tgaccactgt ctccggtggc tcgtcggaag ctgggttagt gtgcgagcac 1020ttgctccagc ttgaaggtgg gtctgaggta ttgagggcaa aaacagagtg gaggcctaag 1080cttagtttca gagggattag tgtgataccc gcagaatcga gtgtctaa 112831128DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 3ttagcttctg ctttctgctc gatgaaagct gtaatgttgg ctcgtgatgg caggggcttg 60aaacccagga gcagtgattt gcagctgagg gcgggaaatg cacaaacctc tttgaagatg 120atcaatggga ccaagttcag ttacacagag agcttgaaaa agttgcctga ctggagcatg 180ctctttgcag tgatcacgac catcttttcg gctgctgaga agcagtggac caatctagag 240tggaagccga agccgaatcc accccagttg cttgatgacc attttgggcc gcatgggtta 300gttttcaggc gcacctttgc catcagatcg tatgaggtgg gacctgaccg ctccacatct 360atagtggctg ttatgaatca cttgcaggag gctgcactta atcatgcgaa gagtgtggga 420attctaggag atggattcgg tacgacgcta gagatgagta agagagatct gatatgggtt 480gtgaaacgca cgcatgttgc tgtggaacgg taccctgctt ggggtgatac tgttgaagta 540gagtgctggg ttggtgcatc gggaaataat ggcaggcgcc atgatttcct tgtccgggac 600tgcaaaacag gcgaaattct tacaagatgt accagtcttt cggtgatgat gaatacaagg 660acaaggaggt tgtccaaaat ccctgaagaa gttagagggg agatagggcc tgcattcatt 720gataatgtgg ctgtcaagga cgaggaaatt aagaaaccac agaagctcaa tgacagcact 780gcagattaca tccaaggagg attgactcct cgatggaatg atttggatat caatcagcac 840gttaacaaca tcaaatacgt tgactggatt cttgagactg tcccagactc aatctttgag 900agtcatcata tttccagctt cactattgaa tacaggagag agtgcacgag ggatagcgtg 960ctgcagtccc tgaccactgt ctccggtggc tcgtcggaag ctgggttagt gtgcgagcac 1020ttgctccagc ttgaaggtgg gtctgaggta ttgagggcaa aaacagagtg gaggcctaag 1080cttagtttca gagggattag tgtgataccc gcagaatcga gtgtctaa 11284357PRTCinnamomum camphora 4Met Val Thr Thr Ser Leu Ala Ser Ala Tyr Phe Ser Met Lys Ala Val 1 5 10 15 Met Leu Ala Pro Asp Gly Arg Gly Ile Lys Pro Arg Ser Ser Gly Leu 20 25 30 Gln Val Arg Ala Gly Asn Glu Arg Asn Ser Cys Lys Val Ile Asn Gly 35 40 45 Thr Lys Val Lys Asp Thr Glu Gly Leu Lys Gly Cys Ser Thr Leu Gln 50 55 60 Gly Gln Ser Met Leu Asp Asp His Phe Gly Leu His Gly Leu Val Phe 65 70 75 80 Arg Arg Thr Phe Ala Ile Arg Cys Tyr Glu Val Gly Pro Asp Arg Ser 85 90 95 Thr Ser Ile Met Ala Val Met Asn His Leu Gln Glu Ala Ala Arg Asn 100 105 110 His Ala Glu Ser Leu Gly Leu Leu Gly Asp Gly Phe Gly Glu Thr Leu 115 120 125 Glu Met Ser Lys Arg Asp Leu Ile Trp Val Val Arg Arg Thr His Val 130 135 140 Ala Val Glu Arg Tyr Pro Ala Trp Gly Asp Thr Val Glu Val Glu Ala 145 150 155 160 Trp Val Gly Ala Ser Gly Asn Thr Gly Met Arg Arg Asp Phe Leu Val 165 170 175 Arg Asp Cys Lys Thr Gly His Ile Leu Thr Arg Cys Thr Ser Val Ser 180 185 190 Val Met Met Asn Met Arg Thr Arg Arg Leu Ser Lys Ile Pro Gln Glu 195 200 205 Val Arg Ala Glu Ile Asp Pro Leu Phe Ile Glu Lys Val Ala Val Lys 210 215 220 Glu Gly Glu Ile Lys Lys Leu Gln Lys Leu Asn Asp Ser Thr Ala Asp 225 230 235 240 Tyr Ile Gln Gly Gly Trp Thr Pro Arg Trp Asn Asp Leu Asp Val Asn 245 250 255 Gln His Val Asn Asn Ile Ile Tyr Val Gly Trp Ile Phe Lys Ser Val 260 265 270 Pro Asp Ser Ile Ser Glu Asn His His Leu Ser Ser Ile Thr Leu Glu 275 280 285 Tyr Arg Arg Glu Cys Thr Arg Gly Asn Lys Leu Gln Ser Leu Thr Thr 290 295 300 Val Cys Gly Gly Ser Ser Glu Ala Gly Ile Ile Cys Glu His Leu Leu 305 310 315 320 Gln Leu Glu Asp Gly Ser Glu Val Leu Arg Ala Arg Thr Glu Trp Arg 325 330 335 Pro Lys His Thr Asp Ser Phe Gln Gly Ile Ser Glu Arg Phe Pro Gln 340 345 350 Gln Glu Pro His Lys 355 51074DNACinnamomum camphora 5atggtcacca cctctttagc ttccgcttac ttctcgatga aagctgtaat gttggctcct 60gacggcaggg gcataaagcc caggagcagt ggtttgcagg tgagggcggg aaatgaacga 120aactcttgca aggtgatcaa tgggaccaag gtcaaagaca cggagggctt gaaagggtgc 180agcacgttgc aaggccagag catgcttgat gaccattttg gtctgcatgg gctagttttc 240aggcgcacct ttgcaatcag atgctatgag gttggacctg accgctccac atccataatg 300gctgttatga atcacttgca ggaagctgca cgtaatcatg cggagagtct gggacttcta 360ggagatggat tcggtgagac actggagatg agtaagagag atctgatatg ggttgtgaga 420cgcacgcatg ttgctgtgga acggtaccct gcttggggcg atactgttga agtcgaggcc 480tgggtgggtg catcaggtaa cactggcatg cgccgcgatt tccttgtccg cgactgcaaa 540actggccaca ttcttacaag atgtaccagt gtttcagtga tgatgaatat gaggacaagg 600agattgtcca aaattcccca agaagttaga gcggagattg accctctttt cattgaaaag 660gttgctgtca aggaagggga aattaaaaaa ttacagaagt tgaatgatag cactgcagat 720tacattcaag ggggttggac tcctcgatgg aatgatttgg atgtcaatca gcacgtgaac 780aatatcatat acgttggctg gatttttaag agcgtcccag actctatctc tgagaatcat 840catctttcta gcatcactct cgaatacagg agagagtgca caaggggcaa caagctgcag 900tccctgacca ctgtttgtgg tggctcgtcg gaagctggga tcatatgtga gcacctactc 960cagcttgagg atgggtctga ggttttgagg gcaagaacag agtggaggcc caagcacacc 1020gatagtttcc aaggcattag tgagagattc ccgcagcaag aaccgcataa gtaa 107461074DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 6atggtgacca cctccctggc ctccgcctac ttctccatga aggccgtgat gctggccccc 60gacggccgcg gcatcaagcc ccgctcctcc ggcctgcagg tgcgcgccgg caacgagcgc 120aactcctgca aggtgatcaa cggcaccaag gtgaaggaca ccgagggcct gaagggctgc 180tccaccctgc agggccagtc catgctggac gaccacttcg gcctgcacgg cctggtgttc 240cgccgcacct tcgccatccg ctgctacgag gtgggccccg accgctccac ctccatcatg 300gccgtgatga accacctgca ggaggccgcc cgcaaccacg ccgagtccct gggcctgctg 360ggcgacggct tcggcgagac cctggagatg tccaagcgcg acctgatctg ggtggtgcgc 420cgcacccacg tggccgtgga gcgctacccc gcctggggcg acaccgtgga ggtggaggcc 480tgggtgggcg cctccggcaa caccggcatg cgccgcgact tcctggtgcg cgactgcaag 540accggccaca tcctgacccg ctgcacctcc gtgtccgtga tgatgaacat gcgcacccgc 600cgcctgtcca agatccccca ggaggtgcgc gccgagatcg accccctgtt catcgagaag 660gtggccgtga aggagggcga gatcaagaag ctgcagaagc tgaacgactc caccgccgac 720tacatccagg gcggctggac cccccgctgg aacgacctgg acgtgaacca gcacgtgaac 780aacatcatct acgtgggctg gatcttcaag tccgtgcccg actccatctc cgagaaccac 840cacctgtcct ccatcaccct ggagtaccgc cgcgagtgca cccgcggcaa caagctgcag 900tccctgacca ccgtgtgcgg cggctcctcc gaggccggca tcatctgcga gcacctgctg 960cagctggagg acggctccga ggtgctgcgc gcccgcaccg agtggcgccc caagcacacc 1020gactccttcc agggcatctc cgagcgcttc ccccagcagg agccccacaa gtga 10747419PRTCinnamomum camphora 7Met Val Ala Thr Ala Ala Ala Ser Ala Phe Phe Pro Val Gly Ala Pro 1 5 10 15 Ala Thr Ser Ser Ala Thr Ser Ala Lys Ala Ser Met Met Pro Asp Asn 20 25 30 Leu Asp Ala Arg Gly Ile Lys Pro Lys Pro Ala Ser Ser Ser Gly Leu 35 40 45 Gln Val Lys Ala Asn Ala His Ala Ser Pro Lys Ile Asn Gly Ser Lys 50 55 60 Val Ser Thr Asp Thr Leu Lys Gly Glu Asp Thr Leu Thr Ser Ser Pro 65 70 75 80 Ala Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Phe Leu 85 90 95 Ala Ala Ile Thr Thr Ile Phe Leu Ala Ala Glu Lys Gln Trp Thr Asn 100 105 110 Leu Asp Trp Lys Pro Arg Arg Pro Asp Met Leu Ala Asp Pro Phe Gly 115 120 125 Ile Gly Arg Phe Met Gln Asp Gly Leu Ile Phe Arg Gln His Phe Ala 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Leu Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Arg Ser Ala 165 170 175 Gly Leu Leu Gly Asp Gly Phe Gly Ala Thr Pro Glu Met Ser Arg Arg 180 185 190 Asp Leu Ile Trp Val Val Thr Arg Met Gln Val Leu Val Asp Arg Tyr 195 200 205 Pro Ala Trp Gly Asp Ile Val Glu Val Glu Thr Trp Val Gly Ala Ser 210 215 220 Gly Lys Asn Gly Met Arg Arg Asp Trp Leu Val Arg Asp Ser Gln Thr 225 230 235 240 Gly Glu Ile Leu Thr Arg Ala Thr Ser Val Trp Val Met Met Asn Lys 245 250 255 Arg Thr Arg Arg Leu Ser Lys Leu Pro Glu Glu Val Arg Gly Glu Ile 260 265 270 Gly Pro Tyr Phe Ile Glu Asp Val Ala Ile Ile Glu Glu Asp Asn Arg 275 280 285 Lys Leu Gln Lys Leu Asn Glu Asn Thr Ala Asp Asn Val Arg Arg Gly 290 295 300 Leu Thr Pro Arg Trp Ser Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Ala Pro Gly Ser Ile Leu 325 330 335 Glu Ser His Glu Leu Ser Cys Met Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Lys Asp Ser Val Leu Gln Ser Met Thr Ala Val Ser Gly Gly Gly 355 360 365 Ser Ala Ala Gly Gly Ser Pro Glu Ser Ser Val Glu Cys Asp His Leu 370 375 380 Leu Gln Leu Glu Ser Gly Pro Glu Val Val Arg Gly Arg Thr Glu Trp 385 390 395 400 Arg Pro Lys Ser Ala Asn Asn Ser Arg Ser Ile Leu Glu Met Pro Ala 405 410 415 Glu Ser Leu 81254DNACinnamomum camphora 8atggttgcca ccgctgctgc ttctgctttc ttcccggtcg gtgctccggc tacgtcatct 60gcaacttcag ccaaagcgtc gatgatgcct gataatttgg atgccagagg catcaaaccg 120aagccggctt cgtccagcgg cttgcaggtt aaggcaaatg cccatgcctc tcccaagatt 180aatggttcca aggtgagcac ggataccttg aagggggaag acaccttaac ttcctcgccc 240gccccacgga cctttatcaa ccaattgcct gactggagca tgttccttgc tgccatcaca 300actattttct tggctgccga gaagcagtgg acgaatctcg actggaagcc cagaagaccc 360gacatgcttg ctgacccgtt tggcatcggg aggtttatgc aggatgggct gattttcagg 420cagcactttg caatcagatc ttatgagatt ggggctgata gaacggcgtc tatagagact 480ttaatgaatc acttgcagga gactgcactt aatcatgtga ggagtgctgg actcctaggt 540gatggatttg gtgcgacacc tgagatgagt agaagagatc tgatatgggt tgtaacacgt 600atgcaggttc ttgtggaccg ctaccctgct tggggtgata ttgttgaagt agagacctgg 660gttggtgcat ctggaaaaaa tggtatgcgc cgtgattggc ttgttcggga cagccaaact 720ggtgaaattc tcacacgagc taccagtgtt tgggtgatga tgaataaacg gacaaggcga 780ttgtccaaac ttcctgaaga agttagaggg gaaatagggc cttattttat agaagatgtt 840gctatcatag aggaggacaa caggaaacta cagaagctca atgaaaacac tgctgataat 900gttcgaaggg gtttgactcc tcgctggagt gatctggatg ttaatcagca tgtgaacaat 960gtcaaataca ttggttggat tcttgagagt gcaccaggat ccatcttgga gagtcatgag 1020ctttcctgca tgacccttga atacaggaga gaatgtggga aggacagtgt gctgcagtca 1080atgactgctg tctctggtgg aggcagtgca gcaggtggct caccagaatc tagcgttgag 1140tgtgaccact tgctccagct agagagtggg cctgaagttg tgaggggaag aaccgagtgg 1200aggcccaaga gtgctaataa ctcgaggagc atcctggaga tgccggccga gagc 125491260DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 9atggtggcca ccgccgccgc ctccgccttc ttccccgtgg gcgcccccgc cacctcctcc 60gccacctccg ccaaggcctc catgatgccc gacaacctgg acgcccgcgg catcaagccc 120aagcccgcct cctcctccgg cctgcaggtg aaggccaacg cccacgcctc ccccaagatc 180aacggctcca aggtgtccac cgacaccctg aagggcgagg acaccctgac ctcctccccc 240gccccccgca ccttcatcaa ccagctgccc gactggtcca tgttcctggc cgccatcacc 300accatcttcc tggccgccga gaagcagtgg accaacctgg actggaagcc ccgccgcccc 360gacatgctgg ccgacccctt cggcatcggc cgcttcatgc aggacggcct gatcttccgc 420cagcacttcg ccatccgctc ctacgagatc ggcgccgacc gcaccgcctc catcgagacc 480ctgatgaacc acctgcagga gaccgccctg aaccacgtgc gctccgccgg cctgctgggc 540gacggcttcg gcgccacccc cgagatgtcc cgccgcgacc tgatctgggt ggtgacccgc 600atgcaggtgc tggtggaccg ctaccccgcc tggggcgaca tcgtggaggt ggagacctgg 660gtgggcgcct ccggcaagaa cggcatgcgc cgcgactggc tggtgcgcga ctcccagacc 720ggcgagatcc tgacccgcgc cacctccgtg tgggtgatga tgaacaagcg cacccgccgc 780ctgtccaagc tgcccgagga ggtgcgcggc gagatcggcc cctacttcat cgaggacgtg 840gccatcatcg aggaggacaa ccgcaagctg cagaagctga acgagaacac cgccgacaac 900gtgcgccgcg gcctgacccc ccgctggtcc gacctggacg tgaaccagca cgtgaacaac 960gtgaagtaca tcggctggat cctggagtcc gcccccggct ccatcctgga gtcccacgag 1020ctgtcctgca tgaccctgga gtaccgccgc gagtgcggca aggactccgt gctgcagtcc 1080atgaccgccg tgtccggcgg cggctccgcc gccggcggct cccccgagtc ctccgtggag 1140tgcgaccacc tgctgcagct ggagtccggc cccgaggtgg tgcgcggccg caccgagtgg 1200cgccccaagt ccgccaacaa ctcccgctcc atcctggaga tgcccgccga gtccctgtga 126010361PRTCuphea hyssopifolia 10Met Val Ala Thr Asn Ala Ala Ala Phe Ser Ala Tyr Thr Phe Phe Leu 1 5 10 15 Thr Ser Pro Thr His Gly

Tyr Ser Ser Lys Arg Leu Ala Asp Thr Gln 20 25 30 Asn Gly Tyr Pro Gly Thr Ser Leu Lys Ser Lys Ser Thr Pro Pro Pro 35 40 45 Ala Ala Ala Ala Ala Arg Asn Gly Ala Leu Pro Leu Leu Ala Ser Ile 50 55 60 Cys Lys Cys Pro Lys Lys Ala Asp Gly Ser Met Gln Leu Asp Ser Ser 65 70 75 80 Leu Val Phe Gly Phe Gln Phe Tyr Ile Arg Ser Tyr Glu Val Gly Ala 85 90 95 Asp Gln Thr Val Ser Ile Gln Thr Val Leu Asn Tyr Leu Gln Glu Ala 100 105 110 Ala Ile Asn His Val Gln Ser Ala Gly Tyr Phe Gly Asp Ser Phe Gly 115 120 125 Ala Thr Pro Glu Met Thr Lys Arg Asn Leu Ile Trp Val Ile Thr Lys 130 135 140 Met Gln Val Leu Val Asp Arg Tyr Pro Ala Trp Gly Asp Val Val Gln 145 150 155 160 Val Asp Thr Trp Thr Cys Ser Ser Gly Lys Asn Ser Met Gln Arg Asp 165 170 175 Trp Phe Val Arg Asp Leu Lys Thr Gly Asp Ile Ile Thr Arg Ala Ser 180 185 190 Ser Val Trp Val Leu Met Asn Arg Leu Thr Arg Lys Leu Ser Lys Ile 195 200 205 Pro Glu Ala Val Leu Glu Glu Ala Lys Leu Phe Val Met Asn Thr Ala 210 215 220 Pro Thr Val Asp Asp Asn Arg Lys Leu Pro Lys Leu Asp Gly Ser Ser 225 230 235 240 Ala Asp Tyr Val Leu Ser Gly Leu Thr Pro Arg Trp Ser Asp Leu Asp 245 250 255 Met Asn Gln His Val Asn Asn Val Lys Tyr Ile Ala Trp Ile Leu Glu 260 265 270 Ser Val Pro Gln Ser Ile Pro Glu Thr His Lys Leu Ser Ala Ile Thr 275 280 285 Val Glu Tyr Arg Arg Glu Cys Gly Lys Asn Ser Val Leu Gln Ser Leu 290 295 300 Thr Asn Val Ser Gly Asp Gly Ile Thr Cys Gly Asn Ser Ile Ile Glu 305 310 315 320 Cys His His Leu Leu Gln Leu Glu Thr Gly Pro Glu Ile Leu Leu Ala 325 330 335 Arg Thr Glu Trp Ile Ser Lys Glu Pro Gly Phe Arg Gly Ala Pro Ile 340 345 350 Gln Ala Glu Lys Val Tyr Asn Asn Lys 355 360 111086DNACuphea hyssopifolia 11atggttgcca ctaatgctgc tgccttttct gcttatactt tcttccttac ttcaccaact 60catggttact cttccaaacg tctcgccgat actcaaaatg gttatccggg tacctccttg 120aaatcgaaat ccactcctcc accagctgct gctgctgctc gtaacggtgc attgccactg 180ctggcctcca tctgcaaatg ccccaaaaag gctgatggga gtatgcaact agacagctcc 240ttggtcttcg ggtttcaatt ttacattaga tcatatgaag tgggtgcgga tcaaaccgtg 300tcaatacaga cagtactcaa ttacttacag gaggcagcca tcaatcatgt tcagagtgct 360ggctattttg gtgatagttt tggcgccacc ccggaaatga ccaagaggaa cctcatctgg 420gttatcacta agatgcaggt tttggtggat cgctatcccg cttggggcga tgttgttcaa 480gttgatacat ggacctgtag ttctggtaaa aacagcatgc agcgtgattg gttcgtacgg 540gatctcaaaa ctggagatat tataacaaga gcctcgagcg tgtgggtgct gatgaataga 600ctcaccagaa aattatcaaa aattcctgaa gcagttctgg aagaagcaaa actttttgtg 660atgaacactg cccccaccgt agatgacaac aggaagctac caaagctgga tggcagcagt 720gctgattatg tcctctctgg cttaactcct agatggagcg acttagatat gaaccagcat 780gtcaacaatg tgaagtacat agcctggatc cttgagagtg tccctcagag cataccggag 840acacacaagc tgtcagcgat aaccgtggag tacaggagag aatgtggcaa gaacagcgtc 900ctccagtctc tgaccaacgt ctccggggat ggaatcacat gtggaaacag tattatcgag 960tgccaccatt tgcttcaact tgagactggc ccagagattc tactagcgcg gacggagtgg 1020atatccaagg aacctgggtt caggggagct ccaatccagg cagagaaagt ctacaacaac 1080aaataa 1086121086DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 12atggtggcca ccaacgccgc cgccttctcc gcctacacct tcttcctgac ctcccccacc 60cacggctact cctccaagcg cctggccgac acccagaacg gctaccccgg cacctccctg 120aagtccaagt ccaccccccc ccccgccgcc gccgccgccc gcaacggcgc cctgcccctg 180ctggcctcca tctgcaagtg ccccaagaag gccgacggct ccatgcagct ggactcctcc 240ctggtgttcg gcttccagtt ctacatccgc tcctacgagg tgggcgccga ccagaccgtg 300tccatccaga ccgtgctgaa ctacctgcag gaggccgcca tcaaccacgt gcagtccgcc 360ggctacttcg gcgactcctt cggcgccacc cccgagatga ccaagcgcaa cctgatctgg 420gtgatcacca agatgcaggt gctggtggac cgctaccccg cctggggcga cgtggtgcag 480gtggacacct ggacctgctc ctccggcaag aactccatgc agcgcgactg gttcgtgcgc 540gacctgaaga ccggcgacat catcacccgc gcctcctccg tgtgggtgct gatgaaccgc 600ctgacccgca agctgtccaa gatccccgag gccgtgctgg aggaggccaa gctgttcgtg 660atgaacaccg cccccaccgt ggacgacaac cgcaagctgc ccaagctgga cggctcctcc 720gccgactacg tgctgtccgg cctgaccccc cgctggtccg acctggacat gaaccagcac 780gtgaacaacg tgaagtacat cgcctggatc ctggagtccg tgccccagtc catccccgag 840acccacaagc tgtccgccat caccgtggag taccgccgcg agtgcggcaa gaactccgtg 900ctgcagtccc tgaccaacgt gtccggcgac ggcatcacct gcggcaactc catcatcgag 960tgccaccacc tgctgcagct ggagaccggc cccgagatcc tgctggcccg caccgagtgg 1020atctccaagg agcccggctt ccgcggcgcc cccatccagg ccgagaaggt gtacaacaac 1080aagtga 108613415PRTCuphea hyssopifolia 13Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Ala Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Ser Pro Leu Lys Pro Lys Leu Met Ala Asn Gly Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly Leu 50 55 60 Lys Ser Gly Ser Leu Lys Thr Gln Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Asp Gly Phe Gly Arg Thr Leu Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Arg Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Arg Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Lys 355 360 365 Gly Ser Gly Ser Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Pro Ile Ala Ser Gly Glu Thr Ser Pro Gly Asp Ser Ser 405 410 415 141248DNACuphea hyssopifolia 14atggtggcta ccgctgcaag ttcagcattc ttccctgtgc cgtcccccga cgcctcctct 60agacctggaa agctcggcaa tgggtcatcg agcttgagcc ccctcaagcc caaattgatg 120gccaatggcg ggttgcaggt taaggcaaac gccagtgccc ctcctaagat caatggttct 180tcggtcggtc taaagtccgg cagtctcaag actcaggaag acactccttc ggcgcctcct 240ccccggactt ttattaacca gctgcctgat tggagtatgc ttcttgctgc aatcactact 300gtcttcttgg cagcagagaa gcagtggatg atgcttgatt ggaaacccaa gaggcctgac 360atgcttgtgg acccgttcgg attgggaagg attgttcaag atgggcttgt gttcaggcag 420aatttttcga ttaggtccta tgaaataggc gctgatcgca ctgcgtctat agagacggtg 480atgaaccact tgcaggaaac agctctcaat catgttaaga gtgctgggct tcttaatgac 540ggctttggtc gtactcttga gatgtataaa agggacctta tttgggttgt tgcaaaaatg 600caggtcatgg ttaaccgcta tcctacttgg ggcgacacgg ttgaagtgaa tacttgggtt 660gccaagtcag ggaaaaatgg tatgcgtcgt gattggctca taagtgattg caatacagga 720gaaattctta ctagagcatc aagtgtgtgg gtcatgatga atcaaaagac aagaagattg 780tcaaaaattc cagatgaggt tcgacatgag atagagcctc atttcgtgga ctctgctccc 840gtcattgaag atgatgaccg gaaacttccc aagctggatg agaagactgc tgactccatc 900cgcaagggtc taactccgaa gtggaatgac ttggatgtca atcagcacgt caacaacgtg 960aagtacattg ggtggattct tgagagtact ccaccagaag ttctggagac ccaggagtta 1020tgttccctta ccctggaata taggcgggaa tgcggaaggg agagcgtgct ggagtccctc 1080actgctgtgg acccctctgg aaagggctct gggtctcagt tccagcacct tctgcggctt 1140gaggatggag gtgagattgt gaaggggaga actgagtggc gacccaagac tgcaggaatc 1200aatgggccaa tagcatccgg ggagacctca cctggagact cttcttag 1248151248DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 15atggtggcca ccgccgcctc ctccgccttc ttccccgtgc cctcccccga cgcctcctcc 60cgccccggca agctgggcaa cggctcctcc tccctgtccc ccctgaagcc caagctgatg 120gccaacggcg gcctgcaggt gaaggccaac gcctccgccc cccccaagat caacggctcc 180tccgtgggcc tgaagtccgg ctccctgaag acccaggagg acaccccctc cgcccccccc 240ccccgcacct tcatcaacca gctgcccgac tggtccatgc tgctggccgc catcaccacc 300gtgttcctgg ccgccgagaa gcagtggatg atgctggact ggaagcccaa gcgccccgac 360atgctggtgg accccttcgg cctgggccgc atcgtgcagg acggcctggt gttccgccag 420aacttctcca tccgctccta cgagatcggc gccgaccgca ccgcctccat cgagaccgtg 480atgaaccacc tgcaggagac cgccctgaac cacgtgaagt ccgccggcct gctgaacgac 540ggcttcggcc gcaccctgga gatgtacaag cgcgacctga tctgggtggt ggccaagatg 600caggtgatgg tgaaccgcta ccccacctgg ggcgacaccg tggaggtgaa cacctgggtg 660gccaagtccg gcaagaacgg catgcgccgc gactggctga tctccgactg caacaccggc 720gagatcctga cccgcgcctc ctccgtgtgg gtgatgatga accagaagac ccgccgcctg 780tccaagatcc ccgacgaggt gcgccacgag atcgagcccc acttcgtgga ctccgccccc 840gtgatcgagg acgacgaccg caagctgccc aagctggacg agaagaccgc cgactccatc 900cgcaagggcc tgacccccaa gtggaacgac ctggacgtga accagcacgt gaacaacgtg 960aagtacatcg gctggatcct ggagtccacc ccccccgagg tgctggagac ccaggagctg 1020tgctccctga ccctggagta ccgccgcgag tgcggccgcg agtccgtgct ggagtccctg 1080accgccgtgg acccctccgg caagggctcc ggctcccagt tccagcacct gctgcgcctg 1140gaggacggcg gcgagatcgt gaagggccgc accgagtggc gccccaagac cgccggcatc 1200aacggcccca tcgcctccgg cgagacctcc cccggcgact cctcctga 124816427PRTCuphea hyssopifolia 16Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Ala Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Ser Pro Leu Lys Pro Lys Leu Met Ala Asn Gly Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly Leu 50 55 60 Lys Ser Gly Ser Leu Lys Thr Gln Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Asp Gly Phe Gly Arg Thr Leu Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Lys Ser Gln Ile Met Leu Pro Leu 245 250 255 His Tyr Cys Ser Val Trp Val Met Met Asn Gln Lys Thr Arg Arg Leu 260 265 270 Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu Pro His Phe Val 275 280 285 Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Arg Lys Leu Pro Lys Leu 290 295 300 Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu Thr Pro Lys Trp 305 310 315 320 Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val Lys Tyr Ile Gly 325 330 335 Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu Thr Gln Glu Leu 340 345 350 Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Glu Ser Val 355 360 365 Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Lys Gly Ser Gly Ser 370 375 380 Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly Glu Ile Val Lys 385 390 395 400 Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile Asn Gly Pro Ile 405 410 415 Ala Ser Gly Glu Thr Ser Pro Gly Asp Ser Ser 420 425 171284DNACuphea hyssopifolia 17atggtggcta ccgctgcaag ttcagcattc ttccctgtgc cgtcccccga cgcctcctct 60agacctggaa agctcggcaa tgggtcatcg agcttgagcc ccctcaagcc caaattgatg 120gccaatggcg ggttgcaggt taaggcaaac gccagtgccc ctcctaagat caatggttct 180tcggtcggtc taaagtccgg cagtctcaag actcaggaag acactccttc ggcgcctcct 240ccccggactt ttattaacca gctgcctgat tggagtatgc ttcttgctgc aatcactact 300gtcttcttgg cagcagagaa gcagtggatg atgcttgatt ggaaacccaa gaggcctgac 360atgcttgtgg acccgttcgg attgggaagg attgttcaag atgggcttgt gttcaggcag 420aatttttcga ttaggtccta tgaaataggc gctgatcgca ctgcgtctat agagacggtg 480atgaaccact tgcaggaaac agctctcaat catgttaaga gtgctgggct tcttaatgac 540ggctttggtc gtactcttga gatgtataaa agggacctta tttgggttgt tgcaaaaatg 600caggtcatgg ttaaccgcta tcctacttgg ggcgacacgg ttgaagtgaa tacttgggtt 660gccaagtcag ggaaaaatgg tatgcgtcgt gattggctca taagtgattg caatacagga 720gaaattctta ctagagcatc aagtaaaagc caaattatgt tacccttaca ttattgcagt 780gtgtgggtca tgatgaatca aaagacaaga agattgtcaa aaattccaga tgaggttcga 840catgagatag agcctcattt cgtggactct gctcccgtca ttgaagatga tgaccggaaa 900cttcccaagc tggatgagaa gactgctgac tccatccgca agggtctaac tccgaagtgg 960aatgacttgg atgtcaatca gcacgtcaac aacgtgaagt acattgggtg gattcttgag 1020agtactccac cagaagttct ggagacccag gagttatgtt cccttaccct ggaatatagg 1080cgggaatgcg gaagggagag cgtgctggag tccctcactg ctgtggaccc ctctggaaag 1140ggctctgggt ctcagttcca gcaccttctg cggcttgagg atggaggtga gattgtgaag 1200gggagaactg agtggcgacc caagactgca ggaatcaatg ggccaatagc atccggggag 1260acctcacctg gagactcttc ttag 1284181284DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 18atggtggcca ccgccgcctc ctccgccttc ttccccgtgc cctcccccga cgcctcctcc 60cgccccggca agctgggcaa cggctcctcc tccctgtccc ccctgaagcc caagctgatg 120gccaacggcg gcctgcaggt gaaggccaac gcctccgccc cccccaagat caacggctcc 180tccgtgggcc tgaagtccgg ctccctgaag acccaggagg acaccccctc cgcccccccc 240ccccgcacct tcatcaacca gctgcccgac tggtccatgc tgctggccgc catcaccacc 300gtgttcctgg ccgccgagaa gcagtggatg atgctggact ggaagcccaa gcgccccgac 360atgctggtgg accccttcgg cctgggccgc atcgtgcagg acggcctggt gttccgccag 420aacttctcca tccgctccta cgagatcggc gccgaccgca ccgcctccat cgagaccgtg 480atgaaccacc tgcaggagac cgccctgaac cacgtgaagt ccgccggcct gctgaacgac 540ggcttcggcc gcaccctgga gatgtacaag cgcgacctga tctgggtggt ggccaagatg 600caggtgatgg tgaaccgcta ccccacctgg ggcgacaccg tggaggtgaa cacctgggtg 660gccaagtccg gcaagaacgg catgcgccgc gactggctga tctccgactg caacaccggc 720gagatcctga cccgcgcctc ctccaagtcc cagatcatgc tgcccctgca ctactgctcc 780gtgtgggtga tgatgaacca gaagacccgc

cgcctgtcca agatccccga cgaggtgcgc 840cacgagatcg agccccactt cgtggactcc gcccccgtga tcgaggacga cgaccgcaag 900ctgcccaagc tggacgagaa gaccgccgac tccatccgca agggcctgac ccccaagtgg 960aacgacctgg acgtgaacca gcacgtgaac aacgtgaagt acatcggctg gatcctggag 1020tccacccccc ccgaggtgct ggagacccag gagctgtgct ccctgaccct ggagtaccgc 1080cgcgagtgcg gccgcgagtc cgtgctggag tccctgaccg ccgtggaccc ctccggcaag 1140ggctccggct cccagttcca gcacctgctg cgcctggagg acggcggcga gatcgtgaag 1200ggccgcaccg agtggcgccc caagaccgcc ggcatcaacg gccccatcgc ctccggcgag 1260acctcccccg gcgactcctc ctga 128419417PRTCuphea hyssopifolia 19Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Thr Ser Leu 20 25 30 Ser Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Ala Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Val Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys His 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Met Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Ala Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser Ile 405 410 415 Ser 201254DNACuphea hyssopifolia 20atggtggctg ccgaagcaag ttctgcactc ttctccgttc gaaccccggg aacctcccct 60aaacccggga agttcgggaa ttggccaacg agcttgagcg tccccttcaa gtccaaatca 120aaccacaatg gcggctttca ggttaaggca aacgccagtg cccgtcctaa ggctaacggt 180tctgcagtaa gtctaaagtc tggcagcctc gacactcagg aggacacttc atcgtcgtcc 240tctcctcctc ggactttcat taaccagttg cccgactgga gtatgctgct gtccgcgatc 300acgaccgtct tcgtggcggc tgagaagcag tggacgatgc ttgatcggaa atctaagagg 360cccgacatgc tcatggaccc gtttggggtt gacagggttg ttcaggatgg ggctgtgttc 420agacagagtt tttcgattag gtcttacgaa ataggcgctg atcgaacagc ctctatagag 480acgctgatga acatcttcca ggaaacatct ctcaatcatt gtaagagtat cggtcttctc 540aatgacggct ttggtcgtac tcctgagatg tgtaagaggg acctcatttg ggtggttaca 600aaaatgcacg tcgaggttaa tcgctatcct acttggggtg atactatcga ggtcaatact 660tgggtctccg agtcggggaa aaccggtatg ggtcgtgatt ggctgataag tgattgtcat 720acaggagaaa ttctaataag agcaacgagc atgtgtgcta tgatgaatca aaagacgaga 780agattctcaa aatttccata tgaggttcga caggagttgg cgcctcattt tgtggactct 840gctcctgtca ttgaagacta tcaaaaattg cacaagcttg atgtgaagac gggtgattcc 900atttgcaatg gcctaactcc aaggtggaat gacttggatg tcaatcagca cgttaacaat 960gtgaagtaca ttgggtggat tctcgagagt gttccaacgg aagttttcga gacccaggag 1020ctatgtggcc tcacccttga gtataggcgg gaatgcggaa gggacagtgt gctggagtcc 1080gtgaccgcta tggatccatc aaaagaggga gacagatctc tgtaccagca ccttcttcgg 1140cttgaggatg gggctgatat cgcgaagggc agaaccaagt ggcggccgaa gaatgcagga 1200accaatgggg caatatcaac aggaaagact tcaaatggaa actcgatctc ttag 1254211254DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 21atggtggccg ccgaggcctc ctccgccctg ttctccgtgc gcacccccgg cacctccccc 60aagcccggca agttcggcaa ctggcccacc tccctgtccg tgcccttcaa gtccaagtcc 120aaccacaacg gcggcttcca ggtgaaggcc aacgcctccg cccgccccaa ggccaacggc 180tccgccgtgt ccctgaagtc cggctccctg gacacccagg aggacacctc ctcctcctcc 240tccccccccc gcaccttcat caaccagctg cccgactggt ccatgctgct gtccgccatc 300accaccgtgt tcgtggccgc cgagaagcag tggaccatgc tggaccgcaa gtccaagcgc 360cccgacatgc tgatggaccc cttcggcgtg gaccgcgtgg tgcaggacgg cgccgtgttc 420cgccagtcct tctccatccg ctcctacgag atcggcgccg accgcaccgc ctccatcgag 480accctgatga acatcttcca ggagacctcc ctgaaccact gcaagtccat cggcctgctg 540aacgacggct tcggccgcac ccccgagatg tgcaagcgcg acctgatctg ggtggtgacc 600aagatgcacg tggaggtgaa ccgctacccc acctggggcg acaccatcga ggtgaacacc 660tgggtgtccg agtccggcaa gaccggcatg ggccgcgact ggctgatctc cgactgccac 720accggcgaga tcctgatccg cgccacctcc atgtgcgcca tgatgaacca gaagacccgc 780cgcttctcca agttccccta cgaggtgcgc caggagctgg ccccccactt cgtggactcc 840gcccccgtga tcgaggacta ccagaagctg cacaagctgg acgtgaagac cggcgactcc 900atctgcaacg gcctgacccc ccgctggaac gacctggacg tgaaccagca cgtgaacaac 960gtgaagtaca tcggctggat cctggagtcc gtgcccaccg aggtgttcga gacccaggag 1020ctgtgcggcc tgaccctgga gtaccgccgc gagtgcggcc gcgactccgt gctggagtcc 1080gtgaccgcca tggacccctc caaggagggc gaccgctccc tgtaccagca cctgctgcgc 1140ctggaggacg gcgccgacat cgccaagggc cgcaccaagt ggcgccccaa gaacgccggc 1200accaacggcg ccatctccac cggcaagacc tccaacggca actccatctc ctga 125422417PRTCuphea hyssopifolia 22Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Thr Ser Leu 20 25 30 Ser Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Ala Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Ile Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Phe His 225 230 235 240 Thr Gly Asp Ile Leu Ile Arg Ala Thr Ser Val Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Ala Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser Ile 405 410 415 Ser 231254DNACuphea hyssopifolia 23atggtggctg ccgaagcaag ttctgcactc ttctccgttc gaaccccggg aacctcccct 60aaacccggga agttcgggaa ttggccaacg agcttgagcg tccccttcaa gtccaaatca 120aaccacaatg gcggctttca ggttaaggca aacgccagtg cccgtcctaa ggctaacggt 180tctgcagtaa gtctaaagtc tggcagcctc gacactcagg aggacacttc atcgtcgtcc 240tctcctcctc ggactttcat taaccagttg cccgactgga gtatgctgct gtccgcgatc 300acgaccgtct tcgtggcggc tgagaagcag tggacgatgc ttgatcggaa atctaagagg 360cccgacatgc tcatggaccc gtttggggtt gacagggttg ttcaggatgg ggctgtgttc 420agacagagtt tttcgattag gtcttacgaa ataggcgctg atcgaacagc ctctatagag 480acgctgatga acatcttcca ggaaacatct ctcaatcatt gtaagagtat cggtcttctc 540aatgacggct ttggtcgtac tcctgagatg tgtaagaggg acctcatttg ggtggttaca 600aaaatgcaca tcgaggttaa tcgctatcct acttggggtg atactatcga ggtcaatact 660tgggtctccg agtcggggaa aaccggtatg ggtcgtgatt ggctgataag tgattttcat 720acaggagaca ttctaataag agcaacgagc gtgtgtgcta tgatgaatca aaagacgaga 780agattctcaa aatttccata tgaggttcga caggagttag cgcctcattt tgtggactct 840gctccagtca ttgaagacta tcaaaaattg cacaagcttg atgtgaagac gggtgattcc 900atttgcaatg gcctaactcc aaggtggaat gacttggatg tcaatcagca cgttaacaat 960gtgaagtaca ttgggtggat tctcgagagt gttccaacgg aagttttcga gacccaggag 1020ctatgtggcc tcacccttga gtataggcgg gaatgcggaa gggacagtgt gctggagtcc 1080gtgaccgcta tggatccctc aaaagaggga gacagatctc tgtaccagca ccttcttcgg 1140cttgaggatg gggctgatat cgcgaagggc agaaccaagt ggcggccgaa gaatgcagga 1200accaatgggg caatatcaac aggaaagact tcaaatggaa actcgatctc ttag 1254241254DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 24atggtggccg ccgaggcctc ctccgccctg ttctccgtgc gcacccccgg cacctccccc 60aagcccggca agttcggcaa ctggcccacc tccctgtccg tgcccttcaa gtccaagtcc 120aaccacaacg gcggcttcca ggtgaaggcc aacgcctccg cccgccccaa ggccaacggc 180tccgccgtgt ccctgaagtc cggctccctg gacacccagg aggacacctc ctcctcctcc 240tccccccccc gcaccttcat caaccagctg cccgactggt ccatgctgct gtccgccatc 300accaccgtgt tcgtggccgc cgagaagcag tggaccatgc tggaccgcaa gtccaagcgc 360cccgacatgc tgatggaccc cttcggcgtg gaccgcgtgg tgcaggacgg cgccgtgttc 420cgccagtcct tctccatccg ctcctacgag atcggcgccg accgcaccgc ctccatcgag 480accctgatga acatcttcca ggagacctcc ctgaaccact gcaagtccat cggcctgctg 540aacgacggct tcggccgcac ccccgagatg tgcaagcgcg acctgatctg ggtggtgacc 600aagatgcaca tcgaggtgaa ccgctacccc acctggggcg acaccatcga ggtgaacacc 660tgggtgtccg agtccggcaa gaccggcatg ggccgcgact ggctgatctc cgacttccac 720accggcgaca tcctgatccg cgccacctcc gtgtgcgcca tgatgaacca gaagacccgc 780cgcttctcca agttccccta cgaggtgcgc caggagctgg ccccccactt cgtggactcc 840gcccccgtga tcgaggacta ccagaagctg cacaagctgg acgtgaagac cggcgactcc 900atctgcaacg gcctgacccc ccgctggaac gacctggacg tgaaccagca cgtgaacaac 960gtgaagtaca tcggctggat cctggagtcc gtgcccaccg aggtgttcga gacccaggag 1020ctgtgcggcc tgaccctgga gtaccgccgc gagtgcggcc gcgactccgt gctggagtcc 1080gtgaccgcca tggacccctc caaggagggc gaccgctccc tgtaccagca cctgctgcgc 1140ctggaggacg gcgccgacat cgccaagggc cgcaccaagt ggcgccccaa gaacgccggc 1200accaacggcg ccatctccac cggcaagacc tccaacggca actccatctc ctga 125425419PRTCuphea sp. 25Met Val Val Ala Ala Ala Thr Ser Ala Phe Phe Pro Val Pro Ala Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Ser Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Pro Thr Phe Lys Pro Lys Ser Ile Pro Asn Ala Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn 50 55 60 Leu Lys Ser Gly Ser Leu Asn Thr Gln Glu Asp Thr Ser Ser Ser Pro 65 70 75 80 Pro Pro Arg Ala Phe Leu Asn Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Thr Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr Met 100 105 110 Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Ser Val Gly 115 120 125 Leu Lys Cys Ile Val Arg Asp Gly Leu Val Ser Arg Gln Ser Phe Leu 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Leu Met Asn His Leu Gln Glu Thr Ser Ile Asn His Cys Lys Ser Leu 165 170 175 Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Gly Met Cys Lys Asn 180 185 190 Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Met Val Asn Arg Tyr 195 200 205 Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Thr Trp Phe Ser Gln Ser 210 215 220 Gly Lys Ile Gly Met Ala Ser Asp Trp Leu Ile Ser Asp Cys Asn Thr 225 230 235 240 Gly Glu Ile Leu Ile Arg Ala Thr Ser Val Trp Ala Met Met Asn Gln 245 250 255 Lys Thr Arg Arg Phe Ser Arg Leu Pro Tyr Glu Val Arg Gln Glu Leu 260 265 270 Thr Pro His Phe Val Asp Ser Pro His Val Ile Glu Asp Asn Asp Gln 275 280 285 Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Arg Lys Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Ser Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Ile Glu Val Leu 325 330 335 Glu Thr Gln Glu Leu Cys Ser Leu Thr Val Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Met Asp Ser Val Leu Glu Ser Val Thr Ala Val Asp Pro Ser Glu 355 360 365 Asn Gly Gly Arg Ser Gln Tyr Lys His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Thr Asp Ile Val Lys Ser Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Thr Asn Gly Ala Ile Ser Thr Ser Thr Ala Lys Thr Ser Asn Gly Asn 405 410 415 Ser Val Ser 261260DNACuphea sp. 26atggtggtgg ctgcagcaac ttctgcattc ttccccgttc cagccccggg aacctcccct 60aaacccggga agtccggcaa ctggccatcg agcttgagcc ctaccttcaa gcccaagtca 120atccccaatg ccggatttca ggttaaggca aatgccagtg cccatcctaa ggctaacggt 180tctgcagtaa atctaaagtc tggcagcctc aacactcagg aggacacttc gtcgtcccct 240cctccccggg ctttccttaa ccagttgcct gattggagta tgcttctgac tgcaatcacg 300accgtcttcg tggcggcaga gaagcagtgg actatgcttg ataggaaatc taagaggcct 360gacatgctcg tggactcggt tgggttgaag tgtattgttc gggatgggct cgtgtccaga 420cagagttttt tgattagatc ttatgaaata ggcgctgatc gaacagcctc tatagagacg 480ctgatgaacc acttgcagga aacatctatc aatcattgta agagtttggg tcttctcaat 540gacggctttg gtcgtactcc tgggatgtgt aaaaacgacc tcatttgggt gcttacaaaa 600atgcagatca tggtgaatcg ctacccaact tggggcgata ctgttgagat caatacctgg 660ttctctcagt cggggaaaat cggtatggct agcgattggc taataagtga ttgcaacaca 720ggagaaattc ttataagagc aacgagcgtg tgggctatga tgaatcaaaa gacgagaaga 780ttctcaagac ttccatacga ggttcgccag gagttaacgc ctcattttgt ggactctcct 840catgtcattg aagacaatga tcagaaattg cataagtttg atgtgaagac tggtgattcc 900attcgcaagg gtctaactcc gaggtggaac gacttggatg tgaatcagca cgtaagcaac 960gtgaagtaca ttgggtggat tctcgagagt atgccaatag aagttttgga gacacaggag

1020ctatgctctc tcaccgtaga atataggcgg gaatgcggaa tggacagtgt gctggagtcc 1080gtgactgctg tggatccctc agaaaatgga ggccggtctc agtacaagca ccttctgcgg 1140cttgaggatg ggactgatat cgtgaagagc agaactgagt ggcgaccgaa gaatgcagga 1200actaacgggg cgatatcaac atcaacagca aagacttcaa atggaaactc ggtctcttag 1260271260DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 27atggtggtgg ccgccgccac ctccgccttc ttccccgtgc ccgcccccgg cacctccccc 60aagcccggca agtccggcaa ctggccctcc tccctgtccc ccaccttcaa gcccaagtcc 120atccccaacg ccggcttcca ggtgaaggcc aacgcctccg cccaccccaa ggccaacggc 180tccgccgtga acctgaagtc cggctccctg aacacccagg aggacacctc ctcctccccc 240cccccccgcg ccttcctgaa ccagctgccc gactggtcca tgctgctgac cgccatcacc 300accgtgttcg tggccgccga gaagcagtgg accatgctgg accgcaagtc caagcgcccc 360gacatgctgg tggactccgt gggcctgaag tgcatcgtgc gcgacggcct ggtgtcccgc 420cagtccttcc tgatccgctc ctacgagatc ggcgccgacc gcaccgcctc catcgagacc 480ctgatgaacc acctgcagga gacctccatc aaccactgca agtccctggg cctgctgaac 540gacggcttcg gccgcacccc cggcatgtgc aagaacgacc tgatctgggt gctgaccaag 600atgcagatca tggtgaaccg ctaccccacc tggggcgaca ccgtggagat caacacctgg 660ttctcccagt ccggcaagat cggcatggcc tccgactggc tgatctccga ctgcaacacc 720ggcgagatcc tgatccgcgc cacctccgtg tgggccatga tgaaccagaa gacccgccgc 780ttctcccgcc tgccctacga ggtgcgccag gagctgaccc cccacttcgt ggactccccc 840cacgtgatcg aggacaacga ccagaagctg cacaagttcg acgtgaagac cggcgactcc 900atccgcaagg gcctgacccc ccgctggaac gacctggacg tgaaccagca cgtgtccaac 960gtgaagtaca tcggctggat cctggagtcc atgcccatcg aggtgctgga gacccaggag 1020ctgtgctccc tgaccgtgga gtaccgccgc gagtgcggca tggactccgt gctggagtcc 1080gtgaccgccg tggacccctc cgagaacggc ggccgctccc agtacaagca cctgctgcgc 1140ctggaggacg gcaccgacat cgtgaagtcc cgcaccgagt ggcgccccaa gaacgccggc 1200accaacggcg ccatctccac ctccaccgcc aagacctcca acggcaactc cgtgtcctga 126028408PRTCuphea wrightii 28Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Pro Arg Thr Thr Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser 20 25 30 Leu Ser Pro Pro Phe Lys Pro Lys Ser Asn Pro Asn Gly Arg Phe Gln 35 40 45 Val Lys Ala Asn Val Ser Pro His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Leu Glu Asp Pro Pro Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Arg Thr Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Phe Thr 100 105 110 Arg Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Trp Phe 115 120 125 Gly Ser Glu Thr Ile Val Gln Asp Gly Leu Val Phe Arg Glu Arg Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn His Leu Gln Asp Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Val Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Ser Glu Met Cys Thr 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Val Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Val Arg Ala Thr Ser Ala Trp Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Leu Pro Cys Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Cys Lys 290 295 300 Gly Leu Thr Pro Gly Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Thr Glu Val 325 330 335 Leu Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu 340 345 350 Cys Gly Arg Glu Ser Val Val Glu Ser Val Thr Ser Met Asn Pro Ser 355 360 365 Lys Val Gly Asp Arg Ser Gln Tyr Gln His Leu Leu Arg Leu Glu Asp 370 375 380 Gly Ala Asp Ile Met Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala 385 390 395 400 Gly Thr Asn Arg Ala Ile Ser Thr 405 291227DNACuphea wrightii 29atggtggtgg ctgctgcagc aagttctgca ttcttccctg ttccagcacc tagaaccacg 60cctaaacccg ggaagttcgg caattggcca tcgagcttga gcccgccctt caagcccaag 120tcaaacccca atggtagatt tcaggttaag gcaaatgtca gtcctcatcc taaggctaac 180ggttctgcag taagtctaaa gtctggcagc ctcaacactc tggaggaccc tccgtcgtcc 240cctcctcctc ggactttcct taaccagttg cctgattgga gtaggcttcg gactgcaatc 300acgaccgtct tcgtggcggc agagaagcag ttcactaggc tcgatcgaaa atctaagagg 360cctgacatgc tagtggactg gtttgggtca gagactattg ttcaggatgg gctcgtgttc 420agagagagat tttcgatcag gtcttacgaa ataggcgctg atcgaacagc ctctatagag 480acgctgatga accacttgca ggacacatct ctgaatcatt gtaagagtgt gggtcttctc 540aatgacggct ttggtcgtac ctcggagatg tgtacaagag acctcatttg ggtgcttaca 600aaaatgcaga tcgtggtgaa tcgctatcca acttggggcg atactgtcga gatcaatagc 660tggttctccc agtcggggaa aatcggtatg ggtcgcgatt ggctaataag tgattgcaac 720acaggagaaa ttcttgtaag agcaacgagc gcttgggcca tgatgaatca aaagacgaga 780agattctcaa aacttccatg cgaggttcgc caggagatag cgcctcattt tgtggacgct 840cctcctgtca ttgaagacaa tgatcggaaa ttgcataagt ttgatgtgaa gactggtgat 900tccatttgca agggtctaac tccggggtgg aatgacttgg atgtcaatca gcacgtaagc 960aacgtgaagt acattgggtg gattctcgag agtatgccta cagaagtttt ggagacccag 1020gagctatgct ctctcaccct tgaatatagg cgggaatgtg gaagggaaag tgtggtagag 1080tccgtgacct ctatgaatcc ctcaaaagtt ggagaccggt ctcagtacca acaccttctg 1140cggcttgagg atggggctga tatcatgaag ggcagaactg agtggagacc aaagaatgca 1200ggaaccaacc gggcgatatc aacatga 1227301227DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 30atggtggtgg ccgccgccgc ctcctccgcc ttcttccccg tgcccgcccc ccgcaccacc 60cccaagcccg gcaagttcgg caactggccc tcctccctgt cccccccctt caagcccaag 120tccaacccca acggccgctt ccaggtgaag gccaacgtgt ccccccaccc caaggccaac 180ggctccgccg tgtccctgaa gtccggctcc ctgaacaccc tggaggaccc cccctcctcc 240cccccccccc gcaccttcct gaaccagctg cccgactggt cccgcctgcg caccgccatc 300accaccgtgt tcgtggccgc cgagaagcag ttcacccgcc tggaccgcaa gtccaagcgc 360cccgacatgc tggtggactg gttcggctcc gagaccatcg tgcaggacgg cctggtgttc 420cgcgagcgct tctccatccg ctcctacgag atcggcgccg accgcaccgc ctccatcgag 480accctgatga accacctgca ggacacctcc ctgaaccact gcaagtccgt gggcctgctg 540aacgacggct tcggccgcac ctccgagatg tgcacccgcg acctgatctg ggtgctgacc 600aagatgcaga tcgtggtgaa ccgctacccc acctggggcg acaccgtgga gatcaactcc 660tggttctccc agtccggcaa gatcggcatg ggccgcgact ggctgatctc cgactgcaac 720accggcgaga tcctggtgcg cgccacctcc gcctgggcca tgatgaacca gaagacccgc 780cgcttctcca agctgccctg cgaggtgcgc caggagatcg ccccccactt cgtggacgcc 840ccccccgtga tcgaggacaa cgaccgcaag ctgcacaagt tcgacgtgaa gaccggcgac 900tccatctgca agggcctgac ccccggctgg aacgacctgg acgtgaacca gcacgtgtcc 960aacgtgaagt acatcggctg gatcctggag tccatgccca ccgaggtgct ggagacccag 1020gagctgtgct ccctgaccct ggagtaccgc cgcgagtgcg gccgcgagtc cgtggtggag 1080tccgtgacct ccatgaaccc ctccaaggtg ggcgaccgct cccagtacca gcacctgctg 1140cgcctggagg acggcgccga catcatgaag ggccgcaccg agtggcgccc caagaacgcc 1200ggcaccaacc gcgccatctc cacctga 122731416PRTCuphea wrightii 31Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Ala 1 5 10 15 Asp Thr Ser Ser Ser Arg Pro Gly Lys Leu Gly Ser Gly Pro Ser Ser 20 25 30 Leu Ser Pro Leu Lys Pro Lys Ser Ile Pro Asn Gly Gly Leu Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly 50 55 60 Leu Lys Ser Gly Gly Phe Lys Thr Gln Glu Asp Ser Pro Ser Ala Pro 65 70 75 80 Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Ala Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met 100 105 110 Leu Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly 115 120 125 Leu Gly Ser Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Val Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ile Ala 165 170 175 Gly Leu Ser Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg 180 185 190 Asp Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr 195 200 205 Pro Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser 210 215 220 Gly Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr 225 230 235 240 Gly Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln 245 250 255 Lys Thr Arg Arg Leu Ser Lys Ile Pro Asp Glu Val Arg Asn Glu Ile 260 265 270 Glu Pro His Phe Val Asp Ser Ala Pro Val Val Glu Asp Asp Asp Arg 275 280 285 Lys Leu Pro Lys Leu Asp Glu Asn Thr Ala Asp Ser Ile Arg Lys Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu 325 330 335 Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Ala 355 360 365 Glu Gly Tyr Ala Ser Arg Phe Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Gly Glu Ile Val Lys Ala Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Ile Asn Gly Val Val Pro Ser Glu Glu Ser Ser Pro Gly Asp Phe Phe 405 410 415 321251DNACuphea wrightii 32ttggtggcta ccgctgcaag ttctgcattt ttccccgtgc catccgccga cacctcctcc 60tcgagacccg gaaagctcgg cagtggacca tcgagcttga gccccctcaa gcccaaatcg 120atccccaatg gcggcttgca ggttaaggca aacgccagtg cccctcctaa gatcaatggt 180tcctcggtcg gtctaaagtc gggcggtttc aagactcagg aagactctcc ttcggcccct 240cctccgcgga cttttatcaa ccagttgcct gattggagta tgcttcttgc tgcaatcact 300actgtcttct tggctgcaga gaagcagtgg atgatgcttg attggaaacc taagaggcct 360gacatgctcg tggacccgtt cggattggga agtattgttc aggatgggct tgtgttcagg 420cagaattttt caattaggtc ctacgaaata ggcgccgatc gaactgcgtc tatagagacg 480gtgatgaacc atttgcagga aacagctctc aatcatgtca agattgctgg gctttctaat 540gacggctttg gtcgtactcc tgagatgtat aaaagagacc ttatttgggt tgttgcaaaa 600atgcaggtca tggttaaccg ctatcctact tggggtgaca cggttgaagt gaatacttgg 660gttgccaagt cagggaaaaa tggtatgcgt cgtgactggc tcataagtga ttgcaatact 720ggagagattc ttacaagagc atcaagcgtg tgggtcatga tgaatcaaaa gacaagaaga 780ttgtcaaaaa ttccagatga ggttcgaaat gagatagagc ctcattttgt ggactctgct 840cccgtcgttg aagatgatga tcggaaactt cccaagctgg atgagaacac tgctgactcc 900atccgcaagg gtctaactcc gaggtggaat gacttggatg tcaatcagca cgtcaacaac 960gtgaagtaca tcggatggat tcttgagagt actccaccag aagttctgga gacccaggag 1020ttatgctccc tgaccctgga atacaggcgg gaatgtggaa gggagagcgt gctggagtcc 1080ctcactgctg tcgacccgtc tgcagagggc tatgcgtccc ggtttcagca ccttctgcgg 1140cttgaggatg gaggtgagat cgtgaaggcg agaactgagt ggcgacccaa gaatgctgga 1200atcaatgggg tggtaccatc cgaggagtcc tcacctggag acttctttta g 1251331251DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 33atggtggcca ccgccgcctc ctccgccttc ttccccgtgc cctccgccga cacctcctcc 60tcccgccccg gcaagctggg ctccggcccc tcctccctgt cccccctgaa gcccaagtcc 120atccccaacg gcggcctgca ggtgaaggcc aacgcctccg ccccccccaa gatcaacggc 180tcctccgtgg gcctgaagtc cggcggcttc aagacccagg aggactcccc ctccgccccc 240cccccccgca ccttcatcaa ccagctgccc gactggtcca tgctgctggc cgccatcacc 300accgtgttcc tggccgccga gaagcagtgg atgatgctgg actggaagcc caagcgcccc 360gacatgctgg tggacccctt cggcctgggc tccatcgtgc aggacggcct ggtgttccgc 420cagaacttct ccatccgctc ctacgagatc ggcgccgacc gcaccgcctc catcgagacc 480gtgatgaacc acctgcagga gaccgccctg aaccacgtga agatcgccgg cctgtccaac 540gacggcttcg gccgcacccc cgagatgtac aagcgcgacc tgatctgggt ggtggccaag 600atgcaggtga tggtgaaccg ctaccccacc tggggcgaca ccgtggaggt gaacacctgg 660gtggccaagt ccggcaagaa cggcatgcgc cgcgactggc tgatctccga ctgcaacacc 720ggcgagatcc tgacccgcgc ctcctccgtg tgggtgatga tgaaccagaa gacccgccgc 780ctgtccaaga tccccgacga ggtgcgcaac gagatcgagc cccacttcgt ggactccgcc 840cccgtggtgg aggacgacga ccgcaagctg cccaagctgg acgagaacac cgccgactcc 900atccgcaagg gcctgacccc ccgctggaac gacctggacg tgaaccagca cgtgaacaac 960gtgaagtaca tcggctggat cctggagtcc accccccccg aggtgctgga gacccaggag 1020ctgtgctccc tgaccctgga gtaccgccgc gagtgcggcc gcgagtccgt gctggagtcc 1080ctgaccgccg tggacccctc cgccgagggc tacgcctccc gcttccagca cctgctgcgc 1140ctggaggacg gcggcgagat cgtgaaggcc cgcaccgagt ggcgccccaa gaacgccggc 1200atcaacggcg tggtgccctc cgaggagtcc tcccccggcg acttcttctg a 125134417PRTCuphea wrightii 34Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Ala 1 5 10 15 Asp Thr Ser Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Pro Ser Ser 20 25 30 Leu Ser Pro Leu Lys Pro Lys Ser Ile Pro Asn Gly Gly Leu Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly 50 55 60 Leu Lys Ser Gly Ser Phe Lys Thr Gln Glu Asp Ala Pro Ser Ala Pro 65 70 75 80 Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Ala Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met 100 105 110 Leu Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly 115 120 125 Leu Gly Ser Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Val Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ile Ala 165 170 175 Gly Leu Ser Ser Asp Gly Phe Gly Arg Thr Pro Ala Met Ser Lys Arg 180 185 190 Asp Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr 195 200 205 Pro Ala Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser 210 215 220 Gly Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr 225 230 235 240 Gly Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln 245 250 255 Lys Thr Arg Arg Leu Ser Lys Ile Pro Asp Glu Val Arg Asn Glu Ile 260 265 270 Glu Pro His Phe Val Asp Ser Ala Pro Val Val Glu Asp Asp Asp Arg 275 280 285 Lys Leu Pro Lys Leu Asp Glu Asn Thr Ala Asp Ser Ile Arg Lys Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Ala Glu Val Leu 325 330 335 Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly 355 360 365 Glu Gly Asp Gly Ser Lys Phe Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Gly Glu Ile Val Lys Ala Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Ile Asn Gly Val Val Pro Ser Glu Glu Ser Ser Pro Gly Gly Asp Phe 405 410 415 Phe 351254DNACuphea wrightii 35ttggtggcta ccgctgcaag

ttctgcattt ttccccgtac catccgccga cacctcctca 60tcgagacccg gaaagctcgg caatgggcca tcgagcttga gccccctcaa gccgaaatcg 120atccccaatg gcgggttgca ggttaaggca aacgccagtg cccctcctaa gatcaatggt 180tcctcggtcg gtctgaagtc gggcagtttc aagactcagg aagacgctcc ttcggcccct 240cctcctcgga cttttatcaa ccagttgcct gattggagta tgcttcttgc tgcaatcact 300actgtcttct tggctgcaga gaagcagtgg atgatgcttg attggaaacc taagaggcct 360gacatgcttg tcgacccgtt cggattggga agtattgttc aggatgggct tgttttcagg 420cagaatttct cgattaggtc ctacgaaata ggcgctgatc gcactgcgtc tatagagacg 480gtgatgaacc atttgcagga aacagctctc aatcatgtta agattgctgg gctttctagt 540gatggctttg gtcgtactcc tgcgatgtct aaacgggacc tcatttgggt tgttgcgaaa 600atgcaggtca tggttaaccg ctaccctgct tggggtgaca cggttgaagt gaatacttgg 660gttgccaagt cagggaaaaa tggtatgcgt cgtgactggc tcataagtga ttgcaacact 720ggagagattc ttacaagagc atcaagcgtg tgggtcatga tgaatcaaaa gacaagaaga 780ttgtcaaaaa ttccagatga ggttcgaaat gagatagagc ctcattttgt ggactctgcg 840cccgtcgttg aagacgatga ccggaaactt cccaagctgg atgagaacac tgctgactcc 900atccgcaagg gtctaactcc gaggtggaat gacttggatg tcaatcagca cgtcaacaac 960gtgaagtaca ttgggtggat tcttgagagt actccagcag aagttctgga gacccaggaa 1020ttatgttccc tgaccctgga atacaggcgg gaatgtggaa gggagagcgt gctggagtcc 1080ctcactgctg tagatccgtc tggagagggc gatgggtcca agttccagca ccttctgcgg 1140cttgaggatg gaggtgagat cgtgaaggcg agaactgagt ggcgaccaaa gaatgctgga 1200atcaatgggg tggtaccatc cgaggagtcc tcacctggtg gagacttctt ttaa 1254361254DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 36atggtggcca ccgccgcctc ctccgccttc ttccccgtgc cctccgccga cacctcctcc 60tcccgccccg gcaagctggg caacggcccc tcctccctgt cccccctgaa gcccaagtcc 120atccccaacg gcggcctgca ggtgaaggcc aacgcctccg ccccccccaa gatcaacggc 180tcctccgtgg gcctgaagtc cggctccttc aagacccagg aggacgcccc ctccgccccc 240cccccccgca ccttcatcaa ccagctgccc gactggtcca tgctgctggc cgccatcacc 300accgtgttcc tggccgccga gaagcagtgg atgatgctgg actggaagcc caagcgcccc 360gacatgctgg tggacccctt cggcctgggc tccatcgtgc aggacggcct ggtgttccgc 420cagaacttct ccatccgctc ctacgagatc ggcgccgacc gcaccgcctc catcgagacc 480gtgatgaacc acctgcagga gaccgccctg aaccacgtga agatcgccgg cctgtcctcc 540gacggcttcg gccgcacccc cgccatgtcc aagcgcgacc tgatctgggt ggtggccaag 600atgcaggtga tggtgaaccg ctaccccgcc tggggcgaca ccgtggaggt gaacacctgg 660gtggccaagt ccggcaagaa cggcatgcgc cgcgactggc tgatctccga ctgcaacacc 720ggcgagatcc tgacccgcgc ctcctccgtg tgggtgatga tgaaccagaa gacccgccgc 780ctgtccaaga tccccgacga ggtgcgcaac gagatcgagc cccacttcgt ggactccgcc 840cccgtggtgg aggacgacga ccgcaagctg cccaagctgg acgagaacac cgccgactcc 900atccgcaagg gcctgacccc ccgctggaac gacctggacg tgaaccagca cgtgaacaac 960gtgaagtaca tcggctggat cctggagtcc acccccgccg aggtgctgga gacccaggag 1020ctgtgctccc tgaccctgga gtaccgccgc gagtgcggcc gcgagtccgt gctggagtcc 1080ctgaccgccg tggacccctc cggcgagggc gacggctcca agttccagca cctgctgcgc 1140ctggaggacg gcggcgagat cgtgaaggcc cgcaccgagt ggcgccccaa gaacgccggc 1200atcaacggcg tggtgccctc cgaggagtcc tcccccggcg gcgacttctt ctga 125437413PRTCuphea wrightii 37Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Pro Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Asp Asn Gly Gly Phe His Val Lys Ala Asn 35 40 45 Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn Leu Lys Ser 50 55 60 Gly Ser Leu Glu Thr Pro Pro Arg Ser Phe Ile Asn Gln Leu Pro Asp 65 70 75 80 Leu Ser Val Leu Leu Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu 85 90 95 Lys Gln Trp Lys Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp 100 105 110 Arg Ile Phe Gln Asp Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg 115 120 125 Ser Tyr Glu Ile Gly Val Asp Arg Thr Ala Ser Ile Glu Thr Leu Met 130 135 140 Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu 145 150 155 160 Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu 165 170 175 Ile Trp Val Val Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr 180 185 190 Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys 195 200 205 Asn Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu 210 215 220 Ile Leu Ile Arg Ala Thr Ser Val Trp Ala Met Met Asn Gln Asn Thr 225 230 235 240 Arg Arg Leu Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro 245 250 255 His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Gln Lys Leu Gln 260 265 270 Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Arg Asp Gly Leu Thr Pro 275 280 285 Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val Lys Tyr 290 295 300 Ile Gly Trp Ile Leu Lys Ser Val Pro Ile Glu Val Phe Glu Thr Gln 305 310 315 320 Glu Leu Cys Gly Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp 325 330 335 Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ala Lys Glu Gly Asp 340 345 350 Arg Cys Val Tyr Gln His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile 355 360 365 Thr Ile Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Gly 370 375 380 Ala Met Ser Ser Gly Lys Thr Ser Asn Gly Asn Cys Leu Ile Glu Gly 385 390 395 400 Arg Gly Trp Gln Pro Phe Arg Val Val Arg Leu Ile Phe 405 410 381242DNACuphea wrightii 38atggtggctg ccgcagcaag ttctgcattc ttctctgttc caaccccggg aacgccccct 60aaacccggga agttcggtaa ctggccatcg agcttgagcg tccccttcaa gcccgacaat 120ggtggctttc atgtcaaggc aaacgccagt gcccatccta aggctaatgg ttctgcggta 180aatctaaagt ctggcagcct cgagactcct cctcggagtt tcattaacca gctgccggac 240ttgagtgtgc ttctgtccaa aatcacgact gtcttcgggg cggctgagaa gcagtggaag 300aggcccggca tgctcgtgga accgtttggg gttgacagga tttttcagga tggtgttttt 360ttcagacaga gtttttctat caggtcttac gaaataggcg ttgatcgaac agcctcgata 420gagacactga tgaacatctt ccaggaaaca tctttgaatc attgcaagag tatcggtctt 480ctcaacgatg gctttggtcg tactcctgag atgtgtaaga gggacctcat ttgggtggtt 540acgaaaattc aggtcgaggt gaatcgctat cctacttggg gtgacactat cgaagtcaat 600acttgggtct cggagtcggg gaaaaacggt atgggtcggg attggctgat aagtgattgc 660cgtactggag agattcttat aagagcaacg agcgtgtggg cgatgatgaa tcaaaacacg 720agaagattgt caaaatttcc atatgaggtt cgacaggaga tagcgcctca ttttgtggac 780tctgctcctg tcattgaaga cgatcaaaag ttgcagaagc ttgatgtgaa gacaggtgat 840tccattcgcg atggtctaac tccgagatgg aatgacttgg atgtcaatca acacgttaac 900aatgtgaagt acattggatg gattctcaag agtgttccaa tagaagtttt cgagacacag 960gagctatgcg gcgtcacact tgaatatagg cgggaatgcg gaagggacag tgtgctggag 1020tcagtgaccg ctatggatcc agcaaaagag ggagaccggt gtgtgtacca gcaccttctt 1080cggcttgagg atggagctga tatcactata ggcagaaccg agtggcggcc gaagaatgca 1140ggagccaatg gtgcaatgtc atcaggaaag acttcaaatg gaaactgtct catagaagga 1200aggggttggc aacctttccg agttgtgcgt ttaattttct ga 1242391242DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 39atggtggccg ccgccgcctc ctccgccttc ttctccgtgc ccacccccgg cacccccccc 60aagcccggca agttcggcaa ctggccctcc tccctgtccg tgcccttcaa gcccgacaac 120ggcggcttcc acgtgaaggc caacgcctcc gcccacccca aggccaacgg ctccgccgtg 180aacctgaagt ccggctccct ggagaccccc ccccgctcct tcatcaacca gctgcccgac 240ctgtccgtgc tgctgtccaa gatcaccacc gtgttcggcg ccgccgagaa gcagtggaag 300cgccccggca tgctggtgga gcccttcggc gtggaccgca tcttccagga cggcgtgttc 360ttccgccagt ccttctccat ccgctcctac gagatcggcg tggaccgcac cgcctccatc 420gagaccctga tgaacatctt ccaggagacc tccctgaacc actgcaagtc catcggcctg 480ctgaacgacg gcttcggccg cacccccgag atgtgcaagc gcgacctgat ctgggtggtg 540accaagatcc aggtggaggt gaaccgctac cccacctggg gcgacaccat cgaggtgaac 600acctgggtgt ccgagtccgg caagaacggc atgggccgcg actggctgat ctccgactgc 660cgcaccggcg agatcctgat ccgcgccacc tccgtgtggg ccatgatgaa ccagaacacc 720cgccgcctgt ccaagttccc ctacgaggtg cgccaggaga tcgcccccca cttcgtggac 780tccgcccccg tgatcgagga cgaccagaag ctgcagaagc tggacgtgaa gaccggcgac 840tccatccgcg acggcctgac cccccgctgg aacgacctgg acgtgaacca gcacgtgaac 900aacgtgaagt acatcggctg gatcctgaag tccgtgccca tcgaggtgtt cgagacccag 960gagctgtgcg gcgtgaccct ggagtaccgc cgcgagtgcg gccgcgactc cgtgctggag 1020tccgtgaccg ccatggaccc cgccaaggag ggcgaccgct gcgtgtacca gcacctgctg 1080cgcctggagg acggcgccga catcaccatc ggccgcaccg agtggcgccc caagaacgcc 1140ggcgccaacg gcgccatgtc ctccggcaag acctccaacg gcaactgcct gatcgagggc 1200cgcggctggc agcccttccg cgtggtgcgc ctgatcttct ga 124240410PRTCuphea heterophylla 40Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Ser Thr Lys Pro Gly Asn Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Glu Ser Asn His Asn Gly Gly Phe Arg Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn 50 55 60 Leu Lys Ser Gly Ser Leu Glu Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Ile Lys Gln Leu Pro Asp Trp Gly Met Leu 85 90 95 Leu Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu Arg Gln Trp Lys 100 105 110 Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp Arg Ile Phe Gln 115 120 125 Asp Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg Ser Tyr Glu Ile 130 135 140 Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Leu Met Asn Ile Phe Gln 145 150 155 160 Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu Leu Asn Asp Gly 165 170 175 Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu Ile Trp Val Val 180 185 190 Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr Trp Gly Asp Thr 195 200 205 Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys Asn Gly Met Gly 210 215 220 Arg Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu Ile Leu Ile Arg 225 230 235 240 Ala Thr Ser Val Trp Ala Met Met Asn Arg Lys Thr Arg Arg Leu Ser 245 250 255 Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro His Phe Val Asp 260 265 270 Ser Ala Pro Val Ile Glu Asp Asp Lys Lys Leu His Lys Leu Asp Val 275 280 285 Lys Thr Gly Asp Ser Ile Arg Lys Gly Leu Thr Pro Arg Trp Asn Asp 290 295 300 Leu Asp Val Asn Gln His Val Asn Asn Val Lys Tyr Ile Gly Trp Ile 305 310 315 320 Leu Lys Ser Val Pro Ala Glu Val Phe Glu Thr Gln Glu Leu Cys Gly 325 330 335 Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp Ser Val Leu Glu 340 345 350 Ser Val Thr Ala Met Asp Thr Ala Lys Glu Gly Asp Arg Ser Leu Tyr 355 360 365 Gln His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile Thr Ile Gly Arg 370 375 380 Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Gly Ala Ile Ser Thr 385 390 395 400 Gly Lys Thr Ser Asn Glu Asn Ser Val Ser 405 410 411233DNACuphea heterophylla 41atggtggctg ccgcagcaag ttctgcattc ttctccgttc caaccccggg aacctccact 60aaacccggga acttcggcaa ttggccatcg agcttgagcg tccccttcaa gcccgaatca 120aaccacaatg gtggctttcg ggtcaaggca aacgccagtg ctcatcctaa ggctaacggt 180tctgcagtaa atctaaagtc tggcagcctc gagactcagg aggacacttc atcgtcgtcc 240cctcctcctc ggacttttat taagcagttg cccgactggg gtatgcttct gtccaaaatc 300acgactgtct tcggggcggc tgagaggcag tggaagaggc ccggcatgct tgtggaaccg 360tttggggttg acaggatttt tcaggatggg gtttttttca gacagagttt ttcgatcagg 420tcttacgaaa taggcgctga tcgaacagcc tcaatagaga cgctgatgaa catcttccag 480gaaacatctc tgaatcattg taagagtatc ggtcttctca atgacggctt tggtcgtact 540cctgagatgt gtaagaggga cctcatttgg gtggttacga aaattcaggt cgaggtgaat 600cgctatccta cttggggtga tactattgag gtcaatactt gggtctcaga gtcggggaaa 660aacggtatgg gtcgtgattg gctgataagc gattgccgta ccggagaaat tcttataaga 720gcaacgagcg tgtgggctat gatgaatcga aagacgagaa gattgtcaaa atttccatat 780gaggttcgac aggagatagc gcctcatttt gtggactctg ctcctgtcat tgaagacgat 840aaaaaattgc acaagcttga tgttaagacg ggtgattcca ttcgcaaggg tctaactcca 900aggtggaatg acttggatgt caatcagcac gttaacaatg tgaagtacat tgggtggatt 960ctcaagagtg ttccagcaga agttttcgag acccaggagc tatgcggagt cacccttgag 1020tacaggcggg aatgtggaag ggacagtgtg ctggagtccg tgaccgctat ggataccgca 1080aaagagggag accggtctct gtaccagcac cttcttcggc ttgaggatgg ggctgatatc 1140accataggca gaaccgagtg gcggccgaag aatgcaggag ccaatggggc aatatcaaca 1200ggaaagactt caaatgaaaa ctctgtctct tag 1233421233DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 42atggtggccg ccgccgcctc ctccgccttc ttctccgtgc ccacccccgg cacctccacc 60aagcccggca acttcggcaa ctggccctcc tccctgtccg tgcccttcaa gcccgagtcc 120aaccacaacg gcggcttccg cgtgaaggcc aacgcctccg cccaccccaa ggccaacggc 180tccgccgtga acctgaagtc cggctccctg gagacccagg aggacacctc ctcctcctcc 240cccccccccc gcaccttcat caagcagctg cccgactggg gcatgctgct gtccaagatc 300accaccgtgt tcggcgccgc cgagcgccag tggaagcgcc ccggcatgct ggtggagccc 360ttcggcgtgg accgcatctt ccaggacggc gtgttcttcc gccagtcctt ctccatccgc 420tcctacgaga tcggcgccga ccgcaccgcc tccatcgaga ccctgatgaa catcttccag 480gagacctccc tgaaccactg caagtccatc ggcctgctga acgacggctt cggccgcacc 540cccgagatgt gcaagcgcga cctgatctgg gtggtgacca agatccaggt ggaggtgaac 600cgctacccca cctggggcga caccatcgag gtgaacacct gggtgtccga gtccggcaag 660aacggcatgg gccgcgactg gctgatctcc gactgccgca ccggcgagat cctgatccgc 720gccacctccg tgtgggccat gatgaaccgc aagacccgcc gcctgtccaa gttcccctac 780gaggtgcgcc aggagatcgc cccccacttc gtggactccg cccccgtgat cgaggacgac 840aagaagctgc acaagctgga cgtgaagacc ggcgactcca tccgcaaggg cctgaccccc 900cgctggaacg acctggacgt gaaccagcac gtgaacaacg tgaagtacat cggctggatc 960ctgaagtccg tgcccgccga ggtgttcgag acccaggagc tgtgcggcgt gaccctggag 1020taccgccgcg agtgcggccg cgactccgtg ctggagtccg tgaccgccat ggacaccgcc 1080aaggagggcg accgctccct gtaccagcac ctgctgcgcc tggaggacgg cgccgacatc 1140accatcggcc gcaccgagtg gcgccccaag aacgccggcg ccaacggcgc catctccacc 1200ggcaagacct ccaacgagaa ctccgtgtcc tga 123343410PRTCuphea heterophylla 43Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Ser 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Asn Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Glu Ser Ser His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn 50 55 60 Leu Lys Ser Gly Ser Leu Glu Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Ile Lys Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Lys Ile Thr Thr Val Phe Trp Ala Ala Glu Arg Gln Trp Lys 100 105 110 Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp Arg Ile Phe Gln 115 120 125 Asp Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg Ser Tyr Glu Ile 130 135 140 Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Leu Met Asn Ile Phe Gln 145 150 155 160 Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu Leu Asn Asp Gly 165 170 175 Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu Ile Trp Val Val 180 185 190 Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr Trp Gly Asp Thr 195 200 205 Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys Asn Gly Met Gly 210 215 220 Arg Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu Ile Leu Ile Arg 225 230 235 240 Ala Thr Ser Val Trp Ala Met Met Asn Arg Lys Thr Arg Arg Leu Ser 245 250 255 Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro His Phe Val Asp 260 265 270

Ser Ala Pro Val Ile Glu Asp Asp Lys Lys Leu His Lys Leu Asp Val 275 280 285 Lys Thr Gly Asp Phe Ile Arg Lys Gly Leu Thr Pro Arg Trp Asn Asp 290 295 300 Phe Asp Val Asn Gln His Val Asn Asn Val Lys Tyr Ile Gly Trp Ile 305 310 315 320 Leu Lys Ser Val Pro Ala Glu Val Phe Glu Thr Gln Glu Leu Cys Gly 325 330 335 Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp Ser Val Leu Glu 340 345 350 Ser Val Thr Ala Met Asp Thr Ala Lys Glu Gly Asp Arg Ser Leu Tyr 355 360 365 Gln His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile Thr Ile Gly Arg 370 375 380 Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Gly Ala Ile Ser Thr 385 390 395 400 Gly Lys Thr Ser Asn Glu Asn Ser Val Ser 405 410 441233DNACuphea heterophylla 44atggtggctg ccgcagcaag ttctgcattc ttctccgttc caacctcggg aacctcccct 60aaacccggga acttcggcaa ttggccatcg agcttgagcg tccccttcaa gcccgaatca 120agccacaatg gtggctttca ggtcaaggca aacgccagtg cccatcctaa ggctaacggt 180tctgcagtaa atctaaagtc tggcagcctc gagactcagg aggacacttc atcgtcgtcc 240cctcctcctc ggacttttat taagcagttg cccgactgga gtatgcttct gtccaaaatc 300acgactgtct tctgggcggc tgagaggcag tggaagaggc ccggcatgct tgtggaaccg 360tttggggttg acaggatttt tcaggatggg gtttttttca gacagagttt ttcgatcagg 420tcttacgaaa taggcgctga tcgaacagcc tcaatagaga cgctgatgaa catcttccag 480gaaacatctc tgaatcattg taagagtatc ggtcttctca atgacggctt tggtcgtact 540cctgagatgt gtaagaggga cctcatttgg gtggttacga aaattcaggt cgaggtgaat 600cgctatccta cttggggtga tactattgag gtcaatactt gggtctcaga gtcggggaaa 660aacggtatgg gtcgtgattg gctgataagc gattgccgta ccggagaaat tcttataaga 720gcaacgagcg tgtgggctat gatgaatcga aagacgagaa gattgtcaaa atttccatat 780gaggttcgac aggagatagc gcctcatttt gtggactctg ctcctgtcat tgaagacgat 840aaaaaattgc acaagcttga tgttaagacg ggtgatttca ttcgcaaggg tctaactcca 900aggtggaatg actttgatgt caatcagcac gttaacaatg tgaagtacat tgggtggatt 960ctcaagagtg ttccagcaga agttttcgag acccaggagc tatgcggagt cacccttgag 1020tataggcggg aatgtggaag ggacagtgtg ctggagtccg tgaccgctat ggataccgca 1080aaagagggag accggtctct gtaccagcac cttcttcggc ttgaggatgg ggctgatatc 1140accataggca gaaccgagtg gcggccgaag aatgcaggag ccaatggggc aatatcaaca 1200ggaaagactt caaatgaaaa ctctgtctct tag 1233451233DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 45atggtggccg ccgccgcctc ctccgccttc ttctccgtgc ccacctccgg cacctccccc 60aagcccggca acttcggcaa ctggccctcc tccctgtccg tgcccttcaa gcccgagtcc 120tcccacaacg gcggcttcca ggtgaaggcc aacgcctccg cccaccccaa ggccaacggc 180tccgccgtga acctgaagtc cggctccctg gagacccagg aggacacctc ctcctcctcc 240cccccccccc gcaccttcat caagcagctg cccgactggt ccatgctgct gtccaagatc 300accaccgtgt tctgggccgc cgagcgccag tggaagcgcc ccggcatgct ggtggagccc 360ttcggcgtgg accgcatctt ccaggacggc gtgttcttcc gccagtcctt ctccatccgc 420tcctacgaga tcggcgccga ccgcaccgcc tccatcgaga ccctgatgaa catcttccag 480gagacctccc tgaaccactg caagtccatc ggcctgctga acgacggctt cggccgcacc 540cccgagatgt gcaagcgcga cctgatctgg gtggtgacca agatccaggt ggaggtgaac 600cgctacccca cctggggcga caccatcgag gtgaacacct gggtgtccga gtccggcaag 660aacggcatgg gccgcgactg gctgatctcc gactgccgca ccggcgagat cctgatccgc 720gccacctccg tgtgggccat gatgaaccgc aagacccgcc gcctgtccaa gttcccctac 780gaggtgcgcc aggagatcgc cccccacttc gtggactccg cccccgtgat cgaggacgac 840aagaagctgc acaagctgga cgtgaagacc ggcgacttca tccgcaaggg cctgaccccc 900cgctggaacg acttcgacgt gaaccagcac gtgaacaacg tgaagtacat cggctggatc 960ctgaagtccg tgcccgccga ggtgttcgag acccaggagc tgtgcggcgt gaccctggag 1020taccgccgcg agtgcggccg cgactccgtg ctggagtccg tgaccgccat ggacaccgcc 1080aaggagggcg accgctccct gtaccagcac ctgctgcgcc tggaggacgg cgccgacatc 1140accatcggcc gcaccgagtg gcgccccaag aacgccggcg ccaacggcgc catctccacc 1200ggcaagacct ccaacgagaa ctccgtgtcc tga 123346418PRTCuphea heterophylla 46Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Ser Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Thr Trp Leu Ser Ser 20 25 30 Ser Ser Pro Ser Tyr Lys Pro Lys Ser Asn Pro Ser Gly Gly Phe Gln 35 40 45 Val Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Gln Glu Gly Thr Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Arg Thr Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Leu Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Lys Pro Asp Met His Val Asp Trp Phe 115 120 125 Gly Leu Glu Ile Ile Val Gln Asp Gly Leu Val Phe Arg Glu Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn His Leu Gln Asp Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Val Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Met Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asn Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Ile Trp Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Leu Pro Asn Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Cys Lys 290 295 300 Gly Leu Thr Pro Glu Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Lys Glu Val 325 330 335 Leu Asp Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu 340 345 350 Cys Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser 355 360 365 Lys Val Gly Asp Arg Ser Gln Tyr Gln His Leu Leu Arg Leu Glu Asp 370 375 380 Gly Thr Asp Ile Met Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala 385 390 395 400 Gly Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser 405 410 415 Val Ser 471257DNACuphea heterophylla 47atggtggtgg ctgctgcagc aagctctgca ttcttccctg ttccggcatc tggaacctcc 60cctaaacccg ggaagttcgg gacttggcta tcgagctcga gcccttccta caagcccaag 120tcaaacccca gtggtggatt tcaggttaag gcaaatgcca gtgctcatcc taaggctaac 180ggttccgcag taagtctaaa gtctggcagc ctcaacactc aggagggcac ttcgtcgtcc 240cctcctcctc ggactttcct taaccagttg cctgattgga gtaggcttcg gactgcaatc 300acgaccgtct tcgtggcggc agagaagcag ttgactatgc tcgatcgaaa gtctaagaag 360cctgacatgc acgtggactg gtttgggttg gagattattg ttcaggatgg gctcgtgttc 420agagagagtt tttcgatcag gtcttacgaa ataggcgctg atcgaacagc ctctatagaa 480acgttgatga accatttgca ggacacatct ttgaaccatt gtaagagtgt gggtcttctc 540aatgacggct ttggtcgtac cccggagatg tgtaaaaggg acctcatttg ggtgcttaca 600aaaatgcaga tcatggtgaa tcgctatcca acttggggcg atactgtcga gatcaatagc 660tggttctccc agtccgggaa aatcggtatg ggtcgcaatt ggctaataag tgattgcaac 720acaggagaaa ttcttataag agcaacgagc atttgggcca tgatgaatca aaagacgaga 780agattctcaa aacttccaaa cgaggttcgc caggagatag cgcctcattt tgtggacgcc 840cctcctgtca ttgaagacaa tgatcgaaaa ttgcataagt ttgatgtgaa gactggtgat 900tccatttgca agggtctaac accggagtgg aatgacttgg atgtcaatca gcacgtaagc 960aacgtgaagt acattgggtg gattctcgag agtatgccaa aagaagtttt ggacacccag 1020gagctatgct ctctcaccct tgaatatagg cgggaatgcg gaagggatag tgtgctggag 1080tctgtgaccg ctatggatcc ctcaaaagtt ggagaccgat ctcagtacca gcaccttctg 1140cggcttgaag atgggactga tatcatgaag ggcagaactg agtggcgacc aaagaatgca 1200ggaaccaacg gggctatatc aacaggaaag acttcaaatg gaaactcggt ctcttag 1257481257DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 48atggtggtgg ccgccgccgc ctcctccgcc ttcttccccg tgcccgcctc cggcacctcc 60cccaagcccg gcaagttcgg cacctggctg tcctcctcct ccccctccta caagcccaag 120tccaacccct ccggcggctt ccaggtgaag gccaacgcct ccgcccaccc caaggccaac 180ggctccgccg tgtccctgaa gtccggctcc ctgaacaccc aggagggcac ctcctcctcc 240cccccccccc gcaccttcct gaaccagctg cccgactggt cccgcctgcg caccgccatc 300accaccgtgt tcgtggccgc cgagaagcag ctgaccatgc tggaccgcaa gtccaagaag 360cccgacatgc acgtggactg gttcggcctg gagatcatcg tgcaggacgg cctggtgttc 420cgcgagtcct tctccatccg ctcctacgag atcggcgccg accgcaccgc ctccatcgag 480accctgatga accacctgca ggacacctcc ctgaaccact gcaagtccgt gggcctgctg 540aacgacggct tcggccgcac ccccgagatg tgcaagcgcg acctgatctg ggtgctgacc 600aagatgcaga tcatggtgaa ccgctacccc acctggggcg acaccgtgga gatcaactcc 660tggttctccc agtccggcaa gatcggcatg ggccgcaact ggctgatctc cgactgcaac 720accggcgaga tcctgatccg cgccacctcc atctgggcca tgatgaacca gaagacccgc 780cgcttctcca agctgcccaa cgaggtgcgc caggagatcg ccccccactt cgtggacgcc 840ccccccgtga tcgaggacaa cgaccgcaag ctgcacaagt tcgacgtgaa gaccggcgac 900tccatctgca agggcctgac ccccgagtgg aacgacctgg acgtgaacca gcacgtgtcc 960aacgtgaagt acatcggctg gatcctggag tccatgccca aggaggtgct ggacacccag 1020gagctgtgct ccctgaccct ggagtaccgc cgcgagtgcg gccgcgactc cgtgctggag 1080tccgtgaccg ccatggaccc ctccaaggtg ggcgaccgct cccagtacca gcacctgctg 1140cgcctggagg acggcaccga catcatgaag ggccgcaccg agtggcgccc caagaacgcc 1200ggcaccaacg gcgccatctc caccggcaag acctccaacg gcaactccgt gtcctga 125749418PRTCuphea heterophylla 49Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Pro Gly Thr Thr Ser Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser 20 25 30 Leu Ser Pro Ser Phe Lys Pro Lys Ser Asn Pro Asn Gly Gly Phe Gln 35 40 45 Val Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Lys Glu Asp Thr Pro Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Arg Thr Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Leu Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Lys Pro Asp Met His Val Asp Trp Phe 115 120 125 Gly Leu Glu Ile Ile Val Gln Asp Trp Leu Val Phe Arg Glu Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn His Leu Gln Asp Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Val Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Met Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asn Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Ile Trp Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Leu Pro Asn Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Leu Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Cys Lys 290 295 300 Gly Leu Thr Pro Glu Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Lys Glu Val 325 330 335 Leu Asp Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu 340 345 350 Cys Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser 355 360 365 Lys Val Gly Asp Arg Ser Gln Tyr Gln His Leu Leu Arg Leu Glu Asp 370 375 380 Gly Thr Asp Ile Met Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala 385 390 395 400 Gly Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser 405 410 415 Val Ser 501257DNACuphea heterophylla 50atggtggtgg ctgctgcagc aagttctgca ttcttccctg ttccagcacc tggaaccacg 60tctaaacccg ggaagttcgg caattggcca tcgagcttga gcccttcctt caagcccaag 120tcaaacccca atggtggatt tcaggttaag gcaaatgcca gcgctcatcc taaggctaac 180gggtctgcag taagtctaaa gtctggcagc ctcaacacta aggaggacac tccgtcgtcc 240cctcctcctc ggactttcct taaccagttg cctgattgga gtaggcttcg gactgcaatc 300acgaccgtct tcgtggcggc agagaagcag ttgactatgc tcgatcgaaa gtctaagaag 360cctgacatgc acgtggactg gtttgggttg gagattattg ttcaggattg gctcgtgttc 420agagagagtt tttcgatcag gtcttacgaa ataggcgctg atcgaacagc ctctatagaa 480acgttgatga accatttgca ggacacatct ttgaaccatt gtaagagtgt gggtcttctc 540aatgacggct ttggtcgtac cccggagatg tgtaaaaggg acctcatttg ggtgcttaca 600aaaatgcaga tcatggtgaa tcgctatcca acttggggcg atactgtcga gatcaatagc 660tggttctccc agtccgggaa aatcggtatg ggtcgcaatt ggctaataag tgattgcaac 720acaggagaaa ttcttataag agcaacgagc atttgggcca tgatgaatca aaagacgaga 780agattctcaa aacttccaaa cgaggttcgc caggagatag ctcctcattt tgtggacgcc 840cctcctctca ttgaagacaa tgatcgaaaa ttgcataagt ttgatgtgaa gactggtgat 900tccatttgca agggtctaac accggagtgg aatgacttgg atgtcaatca gcacgtaagc 960aacgtgaagt acattgggtg gattctcgag agtatgccaa aagaagtttt ggacacccag 1020gagctatgct ctctcaccct tgaatatagg cgggaatgcg gaagggacag tgtgctggag 1080tctgtgaccg ctatggatcc ctcaaaagtt ggagaccgat ctcagtacca gcaccttctg 1140cggcttgaag atgggactga tatcatgaag ggcagaactg agtggcgacc aaagaatgca 1200ggaaccaacg gggcgatatc aacaggaaag acttcaaatg gaaactcggt ctcttag 1257511257DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 51atggtggtgg ccgccgccgc ctcctccgcc ttcttccccg tgcccgcccc cggcaccacc 60tccaagcccg gcaagttcgg caactggccc tcctccctgt ccccctcctt caagcccaag 120tccaacccca acggcggctt ccaggtgaag gccaacgcct ccgcccaccc caaggccaac 180ggctccgccg tgtccctgaa gtccggctcc ctgaacacca aggaggacac cccctcctcc 240cccccccccc gcaccttcct gaaccagctg cccgactggt cccgcctgcg caccgccatc 300accaccgtgt tcgtggccgc cgagaagcag ctgaccatgc tggaccgcaa gtccaagaag 360cccgacatgc acgtggactg gttcggcctg gagatcatcg tgcaggactg gctggtgttc 420cgcgagtcct tctccatccg ctcctacgag atcggcgccg accgcaccgc ctccatcgag 480accctgatga accacctgca ggacacctcc ctgaaccact gcaagtccgt gggcctgctg 540aacgacggct tcggccgcac ccccgagatg tgcaagcgcg acctgatctg ggtgctgacc 600aagatgcaga tcatggtgaa ccgctacccc acctggggcg acaccgtgga gatcaactcc 660tggttctccc agtccggcaa gatcggcatg ggccgcaact ggctgatctc cgactgcaac 720accggcgaga tcctgatccg cgccacctcc atctgggcca tgatgaacca gaagacccgc 780cgcttctcca agctgcccaa cgaggtgcgc caggagatcg ccccccactt cgtggacgcc 840ccccccctga tcgaggacaa cgaccgcaag ctgcacaagt tcgacgtgaa gaccggcgac 900tccatctgca agggcctgac ccccgagtgg aacgacctgg acgtgaacca gcacgtgtcc 960aacgtgaagt acatcggctg gatcctggag tccatgccca aggaggtgct ggacacccag 1020gagctgtgct ccctgaccct ggagtaccgc cgcgagtgcg gccgcgactc cgtgctggag 1080tccgtgaccg ccatggaccc ctccaaggtg ggcgaccgct cccagtacca gcacctgctg 1140cgcctggagg acggcaccga catcatgaag ggccgcaccg agtggcgccc caagaacgcc 1200ggcaccaacg gcgccatctc caccggcaag acctccaacg gcaactccgt gtcctga 125752418PRTCuphea heterophylla 52Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Ser Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Thr Trp Leu Ser Ser 20 25 30 Ser Ser Pro Ser Tyr Lys Pro Lys Ser Asn Pro Ser Gly Gly Phe Gln 35 40 45 Val Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Lys Glu Asp Thr Pro Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Asn Arg Leu 85 90 95 Arg Thr Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Leu Thr 100

105 110 Met Leu Asp Arg Lys Ser Lys Lys Pro Asp Met His Val Asp Trp Phe 115 120 125 Gly Leu Glu Ile Ile Val Gln Asp Gly Leu Val Phe Arg Glu Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn His Leu Gln Asp Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Val Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Met Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asn Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Ile Trp Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Leu Pro Asn Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Cys Lys 290 295 300 Gly Leu Thr Pro Glu Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Lys Glu Val 325 330 335 Leu Asp Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu 340 345 350 Cys Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser 355 360 365 Lys Val Gly Asp Arg Ser Gln Tyr Gln His Leu Leu Arg Leu Glu Asp 370 375 380 Gly Thr Asp Ile Met Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala 385 390 395 400 Gly Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser 405 410 415 Val Ser 531257DNACuphea heterophylla 53atggtggtgg ctgctgcagc aagctctgca ttcttccctg ttccggcatc tggaacctcc 60cctaaacccg ggaagttcgg gacttggcta tcgagctcga gcccttccta caagcccaag 120tcaaacccca gtggtggatt tcaggttaag gcaaatgcca gtgctcatcc taaggctaac 180ggttccgcag taagtctaaa gtctggcagc ctcaacacta aggaggacac tccgtcgtcc 240cctcctcctc ggactttcct taaccagttg cctgattgga ataggcttcg gactgcaatc 300acgaccgtct tcgtggcggc agagaagcag ttgactatgc tcgatcgaaa gtctaagaag 360cctgacatgc acgtggactg gtttgggttg gagattattg ttcaggatgg gctcgtgttc 420agagagagtt tttcgatcag gtcttacgaa ataggcgctg atcgaacagc ctctatagaa 480acgttgatga accatttgca ggacacatct ttgaaccatt gtaagagtgt gggtcttctc 540aatgacggct ttggtcgtac cccggagatg tgtaaaaggg acctcatttg ggtgcttaca 600aaaatgcaga tcatggtgaa tcgctatcca acttggggcg atactgtcga gatcaatagc 660tggttctccc agtccgggaa aatcggtatg ggtcgcaatt ggctaataag tgattgcaac 720acaggagaaa ttcttataag agcaacgagc atttgggcca tgatgaatca aaagacgaga 780agattctcaa aacttccaaa cgaggttcgc caggagatag cgcctcattt tgtggacgcc 840cctcctgtca ttgaagacaa tgatcgaaaa ttgcataagt ttgatgtgaa gactggtgat 900tccatttgca agggtctaac accggagtgg aatgacttgg atgtcaatca gcacgtaagc 960aacgtgaagt acattgggtg gattctcgag agtatgccaa aagaagtttt ggacacccag 1020gagctatgct ctctcaccct tgaatatagg cgggaatgcg gaagggacag tgtgctggag 1080tctgtgaccg ctatggatcc ctcaaaagtt ggggaccgat ctcagtacca gcaccttctg 1140cggcttgaag atgggactga tatcatgaag ggcagaactg agtggcgacc aaagaatgca 1200ggaaccaacg gggctatatc aacaggaaag acttcaaatg gaaactcggt ctcttag 1257541257DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 54atggtggtgg ccgccgccgc ctcctccgcc ttcttccccg tgcccgcctc cggcacctcc 60cccaagcccg gcaagttcgg cacctggctg tcctcctcct ccccctccta caagcccaag 120tccaacccct ccggcggctt ccaggtgaag gccaacgcct ccgcccaccc caaggccaac 180ggctccgccg tgtccctgaa gtccggctcc ctgaacacca aggaggacac cccctcctcc 240cccccccccc gcaccttcct gaaccagctg cccgactgga accgcctgcg caccgccatc 300accaccgtgt tcgtggccgc cgagaagcag ctgaccatgc tggaccgcaa gtccaagaag 360cccgacatgc acgtggactg gttcggcctg gagatcatcg tgcaggacgg cctggtgttc 420cgcgagtcct tctccatccg ctcctacgag atcggcgccg accgcaccgc ctccatcgag 480accctgatga accacctgca ggacacctcc ctgaaccact gcaagtccgt gggcctgctg 540aacgacggct tcggccgcac ccccgagatg tgcaagcgcg acctgatctg ggtgctgacc 600aagatgcaga tcatggtgaa ccgctacccc acctggggcg acaccgtgga gatcaactcc 660tggttctccc agtccggcaa gatcggcatg ggccgcaact ggctgatctc cgactgcaac 720accggcgaga tcctgatccg cgccacctcc atctgggcca tgatgaacca gaagacccgc 780cgcttctcca agctgcccaa cgaggtgcgc caggagatcg ccccccactt cgtggacgcc 840ccccccgtga tcgaggacaa cgaccgcaag ctgcacaagt tcgacgtgaa gaccggcgac 900tccatctgca agggcctgac ccccgagtgg aacgacctgg acgtgaacca gcacgtgtcc 960aacgtgaagt acatcggctg gatcctggag tccatgccca aggaggtgct ggacacccag 1020gagctgtgct ccctgaccct ggagtaccgc cgcgagtgcg gccgcgactc cgtgctggag 1080tccgtgaccg ccatggaccc ctccaaggtg ggcgaccgct cccagtacca gcacctgctg 1140cgcctggagg acggcaccga catcatgaag ggccgcaccg agtggcgccc caagaacgcc 1200ggcaccaacg gcgccatctc caccggcaag acctccaacg gcaactccgt gtcctga 125755418PRTCuphea heterophylla 55Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Pro Gly Thr Thr Ser Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser 20 25 30 Leu Ser Pro Ser Phe Lys Pro Lys Ser Asn Pro Asn Gly Gly Phe Gln 35 40 45 Val Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Gln Glu Asp Thr Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Leu Thr Ala Ile Ser Thr Val Phe Val Ala Ala Glu Lys Gln Leu Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Leu Phe 115 120 125 Gly Leu Glu Ser Ile Val Gln Asp Gly Leu Val Phe Arg Glu Ser Tyr 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn His Leu Gln Asp Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Val Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Met Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asn Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Ile Trp Ala Met Met Asn 245 250 255 Gln Asn Thr Arg Arg Phe Ser Lys Leu Pro Asn Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Arg Lys 290 295 300 Gly Leu Thr Pro Gly Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Thr Glu Val 325 330 335 Leu Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu 340 345 350 Cys Gly Arg Glu Ser Val Leu Glu Ser Val Thr Ala Met Asn Pro Ser 355 360 365 Lys Val Gly Asp Arg Ser Gln Tyr Gln His Leu Leu Arg Leu Glu Asp 370 375 380 Gly Ala Asp Ile Met Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala 385 390 395 400 Gly Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser 405 410 415 Val Ser 561257DNACuphea heterophylla 56atggtggtgg ctgctgcagc aagttctgca ttcttccctg ttccagcacc tggaaccacg 60tctaaacccg ggaagttcgg caattggcca tcgagcttga gcccttcctt caagcccaag 120tcaaacccca atggtggatt tcaggttaag gcaaatgcca gtgctcatcc taaggctaac 180ggttctgcgg taagtctaaa gtctggcagc ctcaacactc aggaggacac ttcgtcgtcc 240cctcctcctc ggacattcct taaccagttg cctgattgga gtaggcttct gactgcaatc 300tcgaccgtct tcgtggcggc agagaagcag ttgactatgc tcgatcgaaa atctaagagg 360cctgacatgc tcgtggactt gtttgggttg gagagtattg ttcaggatgg gctcgtgttc 420agagagagtt attcgatcag gtcttacgaa ataggcgctg atcgaacagc ctctatagaa 480acgttgatga accatttgca ggacacatct ttgaaccatt gtaagagtgt gggtcttctc 540aatgacggct ttggtcgtac cccggagatg tgtaaaaggg acctcatttg ggtgcttaca 600aaaatgcaga tcatggtgaa tcgctatcca acttggggcg atactgtcga gatcaatagc 660tggttctccc agtccgggaa aatcggtatg ggtcgcaatt ggctaataag tgattgcaac 720acaggagaaa ttcttataag agcaacgagc atttgggcca tgatgaatca aaatacgaga 780agattctcaa aacttccaaa cgaggttcgc caggagatag cgcctcattt tgttgacgct 840cctcctgtca ttgaagacaa tgatcgaaaa ttgcataagt ttgatgtgaa gactggtgat 900tccattcgca agggtctaac tccggggtgg aatgacttgg atgtcaatca gcacgtaagc 960aacgtgaagt acattgggtg gattctcgag agtatgccaa cagaagtttt ggagacccag 1020gagctatgct ctctcaccct tgaatatagg cgggaatgcg gaagggaaag tgtgctggag 1080tccgtgaccg ctatgaatcc ctcaaaagtt ggagaccggt ctcagtacca gcaccttcta 1140cggcttgagg atggggctga tatcatgaag ggcagaactg agtggcgacc aaagaatgca 1200ggaaccaacg gggcgatatc aacaggaaag acttcaaatg gaaactcggt ctcttag 1257571257DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 57atggtggtgg ccgccgccgc ctcctccgcc ttcttccccg tgcccgcccc cggcaccacc 60tccaagcccg gcaagttcgg caactggccc tcctccctgt ccccctcctt caagcccaag 120tccaacccca acggcggctt ccaggtgaag gccaacgcct ccgcccaccc caaggccaac 180ggctccgccg tgtccctgaa gtccggctcc ctgaacaccc aggaggacac ctcctcctcc 240cccccccccc gcaccttcct gaaccagctg cccgactggt cccgcctgct gaccgccatc 300tccaccgtgt tcgtggccgc cgagaagcag ctgaccatgc tggaccgcaa gtccaagcgc 360cccgacatgc tggtggacct gttcggcctg gagtccatcg tgcaggacgg cctggtgttc 420cgcgagtcct actccatccg ctcctacgag atcggcgccg accgcaccgc ctccatcgag 480accctgatga accacctgca ggacacctcc ctgaaccact gcaagtccgt gggcctgctg 540aacgacggct tcggccgcac ccccgagatg tgcaagcgcg acctgatctg ggtgctgacc 600aagatgcaga tcatggtgaa ccgctacccc acctggggcg acaccgtgga gatcaactcc 660tggttctccc agtccggcaa gatcggcatg ggccgcaact ggctgatctc cgactgcaac 720accggcgaga tcctgatccg cgccacctcc atctgggcca tgatgaacca gaacacccgc 780cgcttctcca agctgcccaa cgaggtgcgc caggagatcg ccccccactt cgtggacgcc 840ccccccgtga tcgaggacaa cgaccgcaag ctgcacaagt tcgacgtgaa gaccggcgac 900tccatccgca agggcctgac ccccggctgg aacgacctgg acgtgaacca gcacgtgtcc 960aacgtgaagt acatcggctg gatcctggag tccatgccca ccgaggtgct ggagacccag 1020gagctgtgct ccctgaccct ggagtaccgc cgcgagtgcg gccgcgagtc cgtgctggag 1080tccgtgaccg ccatgaaccc ctccaaggtg ggcgaccgct cccagtacca gcacctgctg 1140cgcctggagg acggcgccga catcatgaag ggccgcaccg agtggcgccc caagaacgcc 1200ggcaccaacg gcgccatctc caccggcaag acctccaacg gcaactccgt gtcctga 125758418PRTCuphea heterophylla 58Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Ser Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Thr Trp Leu Ser Ser 20 25 30 Ser Ser Pro Ser Tyr Lys Pro Lys Ser Asn Pro Ser Gly Gly Phe Gln 35 40 45 Val Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Gln Glu Asp Thr Ser Ser Ser 65 70 75 80 Pro Pro Pro Gln Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Leu Thr Ala Ile Ser Thr Val Phe Val Ala Ala Glu Lys Gln Leu Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Trp Phe 115 120 125 Gly Leu Glu Ser Ile Val Gln Asp Gly Leu Val Phe Arg Glu Ser Tyr 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Ser Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Val Met Asn Leu Leu Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Met Gly Ile Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Leu Val Asn Arg 195 200 205 Tyr Pro Asn Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asn Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Ile Trp Ala Met Met Asn 245 250 255 Gln Asn Thr Arg Arg Phe Ser Lys Leu Pro Asn Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Arg Lys 290 295 300 Gly Leu Thr Pro Gly Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Thr Glu Val 325 330 335 Leu Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu 340 345 350 Cys Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asn Pro Ser 355 360 365 Lys Val Gly Asp Arg Ser Gln Tyr Gln His Leu Leu Arg Leu Glu Asp 370 375 380 Gly Ala Asp Ile Met Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala 385 390 395 400 Gly Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser 405 410 415 Val Ser 591257DNACuphea heterophylla 59atggtggtgg ctgctgcagc aagctctgca ttcttccctg ttccggcatc tggaacctcc 60cctaaacccg ggaagttcgg gacttggcta tcgagctcga gcccttccta caagcccaag 120tcaaacccca gtggtggatt tcaggttaag gcaaatgcca gtgctcatcc taaggctaac 180ggttctgcag taagtctaaa gtctggcagc ctcaacactc aggaggacac ttcgtcgtcc 240cctcctcctc agacattcct taaccagttg cctgattgga gtaggcttct gacagcaatc 300tcgaccgtct tcgtggcggc agagaagcag ttgactatgc tcgatcgaaa atctaaaagg 360cctgacatgc tcgtggactg gtttgggttg gagagtattg ttcaggatgg gctcgtgttc 420agagagagtt attcgatcag gtcttacgaa ataagcgctg atcgaacagc ctctatagag 480acggtgatga acctcttgca ggaaacatct ctcaatcatt gtaagagtat gggtattctc 540aatgacggct ttggtcgtac cccggagatg tgcaaaaggg acctcatttg ggtgcttaca 600aaaatgcaga tcttggtgaa tcgctatcca aattggggtg atactgtcga gatcaatagc 660tggttctccc agtccgggaa aatcggtatg ggtcgcaatt ggctaataag tgattgcaac 720acaggagaaa ttcttataag agcaacgagc atttgggcca tgatgaatca aaatacgaga 780agattctcaa aacttccaaa cgaggttcgc caggagatag cgcctcattt tgttgacgct 840cctcctgtca ttgaagacaa tgatcgaaaa ttgcataagt ttgatgtgaa gactggtgat 900tccattcgca agggtctaac tccggggtgg aatgacttgg atgtcaatca gcacgtaagc 960aacgtgaagt acattgggtg gattctcgag agtatgccaa cagaagtttt ggagacccag 1020gagctatgct ctctcaccct tgaatatagg cgggaatgcg gaagggacag tgtgctggag 1080tccgtgaccg ctatgaatcc ctcaaaagtt ggagaccggt ctcagtacca gcaccttcta 1140cggcttgagg atggggctga tatcatgaag ggcagaactg agtggcgacc aaagaatgca 1200ggaaccaacg gggcgatatc aacaggaaag acttcaaatg gaaactcggt ctcttag 1257601257DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 60atggtggtgg ccgccgccgc ctcctccgcc ttcttccccg tgcccgcctc cggcacctcc 60cccaagcccg gcaagttcgg cacctggctg tcctcctcct ccccctccta caagcccaag 120tccaacccct ccggcggctt ccaggtgaag gccaacgcct ccgcccaccc caaggccaac 180ggctccgccg tgtccctgaa gtccggctcc ctgaacaccc aggaggacac ctcctcctcc 240cccccccccc agaccttcct gaaccagctg cccgactggt cccgcctgct gaccgccatc 300tccaccgtgt tcgtggccgc cgagaagcag ctgaccatgc tggaccgcaa gtccaagcgc 360cccgacatgc tggtggactg gttcggcctg gagtccatcg tgcaggacgg cctggtgttc 420cgcgagtcct actccatccg ctcctacgag atctccgccg accgcaccgc ctccatcgag 480accgtgatga acctgctgca ggagacctcc ctgaaccact gcaagtccat gggcatcctg 540aacgacggct tcggccgcac ccccgagatg tgcaagcgcg acctgatctg ggtgctgacc 600aagatgcaga tcctggtgaa ccgctacccc aactggggcg acaccgtgga gatcaactcc 660tggttctccc agtccggcaa gatcggcatg ggccgcaact ggctgatctc cgactgcaac 720accggcgaga tcctgatccg cgccacctcc atctgggcca tgatgaacca gaacacccgc 780cgcttctcca agctgcccaa cgaggtgcgc caggagatcg ccccccactt cgtggacgcc

840ccccccgtga tcgaggacaa cgaccgcaag ctgcacaagt tcgacgtgaa gaccggcgac 900tccatccgca agggcctgac ccccggctgg aacgacctgg acgtgaacca gcacgtgtcc 960aacgtgaagt acatcggctg gatcctggag tccatgccca ccgaggtgct ggagacccag 1020gagctgtgct ccctgaccct ggagtaccgc cgcgagtgcg gccgcgactc cgtgctggag 1080tccgtgaccg ccatgaaccc ctccaaggtg ggcgaccgct cccagtacca gcacctgctg 1140cgcctggagg acggcgccga catcatgaag ggccgcaccg agtggcgccc caagaacgcc 1200ggcaccaacg gcgccatctc caccggcaag acctccaacg gcaactccgt gtcctga 125761418PRTCuphea heterophylla 61Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Ser Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Thr Trp Leu Ser Ser 20 25 30 Ser Ser Pro Ser Tyr Lys Pro Lys Ser Asn Pro Ser Gly Gly Phe Gln 35 40 45 Val Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Gln Glu Gly Thr Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Leu Thr Ala Ile Ser Thr Val Phe Val Ala Ala Glu Lys Gln Leu Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Trp Phe 115 120 125 Gly Leu Glu Ser Ile Val Gln Asp Gly Leu Val Phe Arg Glu Ser Tyr 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Ser Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Val Met Asn Leu Leu Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Met Gly Ile Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Leu Val Asn Arg 195 200 205 Tyr Pro Asn Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asn Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Ile Trp Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Leu Pro Asn Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Cys Lys 290 295 300 Gly Leu Thr Pro Glu Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Lys Glu Val 325 330 335 Leu Asp Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu 340 345 350 Cys Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser 355 360 365 Lys Val Gly Asp Arg Ser Gln Tyr Gln His Leu Leu Arg Leu Glu Asp 370 375 380 Gly Thr Asp Ile Met Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala 385 390 395 400 Gly Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser 405 410 415 Val Ser 621257DNACuphea heterophylla 62atggtggtgg ctgctgcagc aagctctgca ttcttccctg ttccggcatc tggaacctcc 60cctaaacccg ggaagttcgg gacttggcta tcgagctcga gcccttccta caagcccaag 120tcaaacccca gtggtggatt tcaggttaaa gcaaatgcca gtgctcatcc taaggctaac 180ggttccgcag taagtctaaa gtctggcagc ctcaacactc aggagggcac ttcgtcgtcc 240cctcctcctc ggacattcct taaccagttg cctgattgga gtaggcttct gactgcaatc 300tcgaccgtct tcgtggcggc agagaagcag ttgactatgc tcgatcgaaa atctaagagg 360cctgacatgc tcgtggactg gtttgggttg gagagtattg ttcaggatgg gctcgtgttc 420agagagagtt attcgatcag gtcttacgaa ataagcgctg atcgaacagc ctctatagag 480acggtgatga acctcttgca ggaaacatct ctcaatcatt gtaagagtat gggtattctc 540aatgacggct ttggtcgtac cccggagatg tgcaaaaggg acctcatttg ggtgcttaca 600aaaatgcaga tcttggtgaa tcgctatcca aattggggtg atactgtcga gatcaatagc 660tggttctccc agtccgggaa aatcggtatg ggtcgcaatt ggctaataag tgattgcaac 720acaggagaaa ttcttataag agcaacgagc atttgggcca tgatgaatca aaagacgaga 780agattctcaa aacttccaaa tgaggttcgc caggagatag cgcctcattt tgtggacgcc 840cctcctgtca ttgaagacaa tgatcgaaaa ttgcataagt ttgatgtgaa gactggtgat 900tccatttgca agggtctaac accggagtgg aacgacttgg atgtcaatca gcacgtaagc 960aacgtgaagt acattgggtg gattctcgag agtatgccaa aagaagtttt ggacacccag 1020gagctatgct ctctcaccct tgaatatagg cgggaatgcg gaagggacag tgtgctggag 1080tctgtgaccg ctatggatcc ctcaaaagtt ggagaccgat ctcagtacca gcaccttctg 1140cggcttgaag atgggactga tatcatgaag ggcagaactg agtggcgacc aaagaatgca 1200ggaaccaacg gggcgatatc aacaggaaag acttcaaatg gaaactcggt ctcttag 1257631257DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 63atggtggtgg ccgccgccgc ctcctccgcc ttcttccccg tgcccgcctc cggcacctcc 60cccaagcccg gcaagttcgg cacctggctg tcctcctcct ccccctccta caagcccaag 120tccaacccct ccggcggctt ccaggtgaag gccaacgcct ccgcccaccc caaggccaac 180ggctccgccg tgtccctgaa gtccggctcc ctgaacaccc aggagggcac ctcctcctcc 240cccccccccc gcaccttcct gaaccagctg cccgactggt cccgcctgct gaccgccatc 300tccaccgtgt tcgtggccgc cgagaagcag ctgaccatgc tggaccgcaa gtccaagcgc 360cccgacatgc tggtggactg gttcggcctg gagtccatcg tgcaggacgg cctggtgttc 420cgcgagtcct actccatccg ctcctacgag atctccgccg accgcaccgc ctccatcgag 480accgtgatga acctgctgca ggagacctcc ctgaaccact gcaagtccat gggcatcctg 540aacgacggct tcggccgcac ccccgagatg tgcaagcgcg acctgatctg ggtgctgacc 600aagatgcaga tcctggtgaa ccgctacccc aactggggcg acaccgtgga gatcaactcc 660tggttctccc agtccggcaa gatcggcatg ggccgcaact ggctgatctc cgactgcaac 720accggcgaga tcctgatccg cgccacctcc atctgggcca tgatgaacca gaagacccgc 780cgcttctcca agctgcccaa cgaggtgcgc caggagatcg ccccccactt cgtggacgcc 840ccccccgtga tcgaggacaa cgaccgcaag ctgcacaagt tcgacgtgaa gaccggcgac 900tccatctgca agggcctgac ccccgagtgg aacgacctgg acgtgaacca gcacgtgtcc 960aacgtgaagt acatcggctg gatcctggag tccatgccca aggaggtgct ggacacccag 1020gagctgtgct ccctgaccct ggagtaccgc cgcgagtgcg gccgcgactc cgtgctggag 1080tccgtgaccg ccatggaccc ctccaaggtg ggcgaccgct cccagtacca gcacctgctg 1140cgcctggagg acggcaccga catcatgaag ggccgcaccg agtggcgccc caagaacgcc 1200ggcaccaacg gcgccatctc caccggcaag acctccaacg gcaactccgt gtcctga 125764418PRTCuphea heterophylla 64Met Val Val Ala Ala Thr Ala Ser Ser Ala Phe Phe Pro Val Pro Val 1 5 10 15 Pro Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Thr Trp Leu Ser Ser 20 25 30 Ser Ser Pro Ser Tyr Lys Pro Lys Ser Asn Pro Ser Gly Gly Phe Gln 35 40 45 Val Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Gln Glu Asp Thr Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Leu Thr Ala Ile Ser Thr Val Phe Val Ala Ala Glu Lys Gln Leu Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Trp Phe 115 120 125 Gly Leu Glu Ser Ile Val Gln Asp Gly Leu Val Phe Arg Glu Ile Tyr 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Ser Ala Asp Arg Thr Thr Ser Ile Glu 145 150 155 160 Thr Val Met Asn Leu Leu Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Met Gly Ile Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Leu Val Asn Arg 195 200 205 Tyr Pro Asn Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asn Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Ile Trp Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Leu Pro Asn Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Cys Lys 290 295 300 Gly Leu Thr Pro Glu Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Lys Glu Val 325 330 335 Leu Asp Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu 340 345 350 Cys Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser 355 360 365 Lys Val Gly Asp Arg Ser Gln Tyr Gln His Leu Leu Arg Leu Glu Asp 370 375 380 Gly Thr Asp Ile Met Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala 385 390 395 400 Gly Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Ala Asn Ser 405 410 415 Val Ser 651257DNACuphea heterophylla 65atggtggtgg ctgctacagc aagttctgca ttcttccctg ttcctgtacc tggaacctcc 60cctaaacccg gaaagttcgg gacttggcta tcgagctcga gcccttccta caagcccaag 120tcaaacccca gtggtggatt tcaggttaag gcaaatgcca gtgctcatcc taaggctaac 180ggttctgcag taagtctaaa gtctggcagc ctcaacactc aggaggacac ttcgtcgtcc 240cctcctcctc ggacattcct taaccagttg cctgattgga gtaggcttct gactgcaatc 300tcgaccgtct tcgtggcggc agagaagcag ttgactatgc tcgatcgaaa atctaagagg 360cctgacatgc tcgtggactg gtttgggttg gagagtattg ttcaggatgg gctcgtgttc 420agagagattt attcgatcag gtcttacgaa ataagcgctg atcgaacaac ctctatagag 480acggtgatga acctcttgca ggaaacatct ctcaatcatt gtaagagtat gggtattctc 540aatgacggct ttggtcgtac cccggagatg tgcaaaaggg acctcatttg ggtgcttaca 600aaaatgcaga tcttggtgaa tcgctatcca aattggggtg atactgtcga gatcaatagc 660tggttctccc agtccgggaa aatcggtatg ggtcgcaatt ggctaataag tgattgcaac 720acaggagaaa ttcttataag agcaacgagc atttgggcca tgatgaatca aaagacgaga 780agattctcaa aacttccaaa cgaggttcgc caggagatag cgcctcattt tgtggacgcc 840cctcctgtca ttgaagacaa tgatcgaaaa ttgcataagt ttgatgtgaa gactggtgat 900tccatttgca agggtctaac accggagtgg aatgacttgg atgtcaatca gcacgtaagc 960aacgtgaagt acattgggtg gattctcgag agtatgccaa aagaagtttt ggacacccag 1020gagctatgct ctctcaccct tgaatatagg cgggaatgcg gaagggacag tgtgctggag 1080tctgtgaccg ctatggatcc ctcaaaagtt ggagaccgat ctcagtacca gcaccttctg 1140cggcttgaag atgggactga tatcatgaag ggcagaactg agtggcgacc aaagaatgca 1200ggaaccaacg gggcgatatc aacaggaaag acttcaaatg caaactcggt ctcttag 1257661257DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 66atggtggtgg ccgccaccgc ctcctccgcc ttcttccccg tgcccgtgcc cggcacctcc 60cccaagcccg gcaagttcgg cacctggctg tcctcctcct ccccctccta caagcccaag 120tccaacccct ccggcggctt ccaggtgaag gccaacgcct ccgcccaccc caaggccaac 180ggctccgccg tgtccctgaa gtccggctcc ctgaacaccc aggaggacac ctcctcctcc 240cccccccccc gcaccttcct gaaccagctg cccgactggt cccgcctgct gaccgccatc 300tccaccgtgt tcgtggccgc cgagaagcag ctgaccatgc tggaccgcaa gtccaagcgc 360cccgacatgc tggtggactg gttcggcctg gagtccatcg tgcaggacgg cctggtgttc 420cgcgagatct actccatccg ctcctacgag atctccgccg accgcaccac ctccatcgag 480accgtgatga acctgctgca ggagacctcc ctgaaccact gcaagtccat gggcatcctg 540aacgacggct tcggccgcac ccccgagatg tgcaagcgcg acctgatctg ggtgctgacc 600aagatgcaga tcctggtgaa ccgctacccc aactggggcg acaccgtgga gatcaactcc 660tggttctccc agtccggcaa gatcggcatg ggccgcaact ggctgatctc cgactgcaac 720accggcgaga tcctgatccg cgccacctcc atctgggcca tgatgaacca gaagacccgc 780cgcttctcca agctgcccaa cgaggtgcgc caggagatcg ccccccactt cgtggacgcc 840ccccccgtga tcgaggacaa cgaccgcaag ctgcacaagt tcgacgtgaa gaccggcgac 900tccatctgca agggcctgac ccccgagtgg aacgacctgg acgtgaacca gcacgtgtcc 960aacgtgaagt acatcggctg gatcctggag tccatgccca aggaggtgct ggacacccag 1020gagctgtgct ccctgaccct ggagtaccgc cgcgagtgcg gccgcgactc cgtgctggag 1080tccgtgaccg ccatggaccc ctccaaggtg ggcgaccgct cccagtacca gcacctgctg 1140cgcctggagg acggcaccga catcatgaag ggccgcaccg agtggcgccc caagaacgcc 1200ggcaccaacg gcgccatctc caccggcaag acctccaacg ccaactccgt gtcctga 125767414PRTCuphea heterophylla 67Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Thr Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Arg Pro Leu Lys Pro Lys Phe Val Ala Asn Ala Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Ser Leu 50 55 60 Lys Ser Cys Ser Leu Lys Thr His Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Glu Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Lys Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Trp Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Lys 355 360 365 Gly Phe Gly Pro Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Thr Ile Ala Ser Gly Glu Thr Ser Pro Gly Asn Ser 405 410 681245DNACuphea heterophylla 68atggtggcca ccgctgcaag ttctgcattc ttcccggtgc cgtccccgga cacctcctct 60agaccgggaa agctcggaaa tgggtcatca agcttgaggc ccctcaagcc caaatttgtt 120gccaatgctg ggctgcaggt taaggcaaac gccagtgccc ctcctaagat caatggttcc 180tcggtcagtc taaagtcttg cagtctcaag actcatgaag acactccttc agctcctcct 240ccgcggactt ttatcaacca gttgcctgat tggagcatgc ttcttgctgc aatcactact 300gtcttcttgg cagcagagaa gcagtggatg atgcttgatt ggaaaccaaa gaggcctgac 360atgcttgtgg acccgttcgg attgggaagg attgttcagg atgggcttgt gttcaggcag 420aatttttcga ttaggtccta tgaaataggc gctgatcgca ctgcatccat agagacggtg 480atgaaccact tgcaggaaac ggctctcaat catgttaaga gtgcggggct tcttaatgaa 540ggctttggtc gtactcctga gatgtataaa agggacctta tttgggttgt cgcgaaaatg 600caggtcatgg ttaaccgcta tcctacttgg ggtgacacgg ttgaagtgaa tacttgggtt 660gccaagtcag ggaaaaatgg tatgcgtcgt gattggctca taagtgattg caatacagga 720gaaattctta caagggcatc aagtgtgtgg gtcatgatga atcaaaagac aagaaaattg 780tcaaagattc cagatgaggt tcggcatgag atagagcctc attttgtgga ctctgctccc 840gtcattgaag acgatgactg gaaacttccc aagctggatg agaaaactgc tgactccatc 900cgcaagggtc taactccgaa gtggaatgac ttggatgtca atcagcacgt caacaacgtg 960aagtacattg ggtggattct tgagagtact ccaccagaag ttctggagac ccaggagtta 1020tgttccctta ccctggaata caggcgggaa tgcggaaggg agagtgtgct ggagtccctc

1080actgctgtgg acccctctgg aaagggcttt gggccccagt ttcagcacct tctgaggctt 1140gaggatggag gtgagatcgt aaaggggaga actgagtggc gacccaagac tgcaggtatc 1200aatgggacga ttgcatctgg ggagacctca cctggaaact cttag 1245691245DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 69atggtggcca ccgccgcctc ctccgccttc ttccccgtgc cctcccccga cacctcctcc 60cgccccggca agctgggcaa cggctcctcc tccctgcgcc ccctgaagcc caagttcgtg 120gccaacgccg gcctgcaggt gaaggccaac gcctccgccc cccccaagat caacggctcc 180tccgtgtccc tgaagtcctg ctccctgaag acccacgagg acaccccctc cgcccccccc 240ccccgcacct tcatcaacca gctgcccgac tggtccatgc tgctggccgc catcaccacc 300gtgttcctgg ccgccgagaa gcagtggatg atgctggact ggaagcccaa gcgccccgac 360atgctggtgg accccttcgg cctgggccgc atcgtgcagg acggcctggt gttccgccag 420aacttctcca tccgctccta cgagatcggc gccgaccgca ccgcctccat cgagaccgtg 480atgaaccacc tgcaggagac cgccctgaac cacgtgaagt ccgccggcct gctgaacgag 540ggcttcggcc gcacccccga gatgtacaag cgcgacctga tctgggtggt ggccaagatg 600caggtgatgg tgaaccgcta ccccacctgg ggcgacaccg tggaggtgaa cacctgggtg 660gccaagtccg gcaagaacgg catgcgccgc gactggctga tctccgactg caacaccggc 720gagatcctga cccgcgcctc ctccgtgtgg gtgatgatga accagaagac ccgcaagctg 780tccaagatcc ccgacgaggt gcgccacgag atcgagcccc acttcgtgga ctccgccccc 840gtgatcgagg acgacgactg gaagctgccc aagctggacg agaagaccgc cgactccatc 900cgcaagggcc tgacccccaa gtggaacgac ctggacgtga accagcacgt gaacaacgtg 960aagtacatcg gctggatcct ggagtccacc ccccccgagg tgctggagac ccaggagctg 1020tgctccctga ccctggagta ccgccgcgag tgcggccgcg agtccgtgct ggagtccctg 1080accgccgtgg acccctccgg caagggcttc ggcccccagt tccagcacct gctgcgcctg 1140gaggacggcg gcgagatcgt gaagggccgc accgagtggc gccccaagac cgccggcatc 1200aacggcacca tcgcctccgg cgagacctcc cccggcaact cctga 124570414PRTCuphea heterophylla 70Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Thr Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Arg Pro Leu Lys Pro Lys Phe Val Ala Asn Ala Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Ser Leu 50 55 60 Lys Ser Gly Ser Leu Lys Thr Gln Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Phe 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Ile Glu Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Lys Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Trp Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Lys 355 360 365 Gly Phe Gly Pro Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Thr Ile Ala Ser Gly Glu Thr Ser Pro Gly Asn Ser 405 410 711245DNACuphea heterophylla 71atggtggcca ccgctgcaag ttctgcattc ttcccggtgc catccccgga cacctcctct 60agaccgggaa agctcggaaa tgggtcatca agcttgaggc ccctcaagcc caaatttgtt 120gccaatgctg ggctgcaggt taaggcaaac gccagtgccc ctcctaagat caatggttcc 180tcggtcagtc taaagtctgg cagtctcaag actcaggaag acactccttc ggctcctcct 240ccgcggactt ttatcaacca gttgcctgat tggagcatgc ttcttgctgc aatcactact 300gtcttcttgg cagcagagaa gcagtggatg atgcttgatt ggaaaccaaa gaggcctgac 360atgcttgtgg acccgttcgg atttggaagg attgttcagg atgggcttgt gttcaggcag 420aatttttcga ttaggtccta tgaaataggc gctgatcgca ctgcatctat agagacggtg 480atgaaccact tgcaggaaac ggctctcaat catgttaaga gtgcggggct tcttattgaa 540ggctttggtc gtactcctga gatgtataaa agggacctta tttgggttgt cgcgaaaatg 600caggtcatgg ttaaccgcta tcctacttgg ggtgacacgg ttgaagtgaa tacttgggtt 660gccaagtcag ggaaaaatgg tatgcgtcgt gattggctca taagtgattg caatacagga 720gaaattctta ctagagcatc aagtgtgtgg gtcatgatga atcaaaagac aagaaaattg 780tcaaagattc cagatgaggt tcggcatgag atagagcctc attttgtgga ctctgctccc 840gtcattgaag acgatgactg gaaacttccc aagctggatg agaaaactgc tgactccatc 900cgcaagggtc taactccgaa gtggaatgac ttggatgtca atcagcacgt caacaacgtg 960aagtacattg ggtggattct tgagagtact ccaccagaag ttctggagac ccaggagtta 1020tgttccctta ccctggaata caggcgggaa tgcggaaggg agagtgtgct ggagtccctc 1080actgctgtgg acccctctgg aaagggcttt gggccccagt ttcagcacct tctgaggctt 1140gaggatggag gtgagatcgt aaaggggaga actgagtggc gacccaagac tgcaggtatc 1200aatgggacga ttgcatctgg ggagacctca cctggaaact cttag 1245721245DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 72atggtggcca ccgccgcctc ctccgccttc ttccccgtgc cctcccccga cacctcctcc 60cgccccggca agctgggcaa cggctcctcc tccctgcgcc ccctgaagcc caagttcgtg 120gccaacgccg gcctgcaggt gaaggccaac gcctccgccc cccccaagat caacggctcc 180tccgtgtccc tgaagtccgg ctccctgaag acccaggagg acaccccctc cgcccccccc 240ccccgcacct tcatcaacca gctgcccgac tggtccatgc tgctggccgc catcaccacc 300gtgttcctgg ccgccgagaa gcagtggatg atgctggact ggaagcccaa gcgccccgac 360atgctggtgg accccttcgg cttcggccgc atcgtgcagg acggcctggt gttccgccag 420aacttctcca tccgctccta cgagatcggc gccgaccgca ccgcctccat cgagaccgtg 480atgaaccacc tgcaggagac cgccctgaac cacgtgaagt ccgccggcct gctgatcgag 540ggcttcggcc gcacccccga gatgtacaag cgcgacctga tctgggtggt ggccaagatg 600caggtgatgg tgaaccgcta ccccacctgg ggcgacaccg tggaggtgaa cacctgggtg 660gccaagtccg gcaagaacgg catgcgccgc gactggctga tctccgactg caacaccggc 720gagatcctga cccgcgcctc ctccgtgtgg gtgatgatga accagaagac ccgcaagctg 780tccaagatcc ccgacgaggt gcgccacgag atcgagcccc acttcgtgga ctccgccccc 840gtgatcgagg acgacgactg gaagctgccc aagctggacg agaagaccgc cgactccatc 900cgcaagggcc tgacccccaa gtggaacgac ctggacgtga accagcacgt gaacaacgtg 960aagtacatcg gctggatcct ggagtccacc ccccccgagg tgctggagac ccaggagctg 1020tgctccctga ccctggagta ccgccgcgag tgcggccgcg agtccgtgct ggagtccctg 1080accgccgtgg acccctccgg caagggcttc ggcccccagt tccagcacct gctgcgcctg 1140gaggacggcg gcgagatcgt gaagggccgc accgagtggc gccccaagac cgccggcatc 1200aacggcacca tcgcctccgg cgagacctcc cccggcaact cctga 124573419PRTCuphea viscosissima 73Met Val Ala Ala Ala Ala Thr Ser Ala Phe Phe Pro Val Pro Ala Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Ser Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Pro Thr Phe Lys Pro Lys Ser Ile Pro Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn 50 55 60 Leu Lys Ser Gly Ser Leu Asn Thr Gln Glu Asp Thr Ser Ser Ser Pro 65 70 75 80 Pro Pro Arg Ala Phe Leu Asn Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Thr Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr Met 100 105 110 Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Ser Val Gly 115 120 125 Leu Lys Ser Ile Val Arg Asp Gly Leu Val Ser Arg His Ser Phe Ser 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Leu Met Asn His Leu Gln Glu Thr Thr Ile Asn His Cys Lys Ser Leu 165 170 175 Gly Leu His Asn Asp Gly Phe Gly Arg Thr Pro Gly Met Cys Lys Asn 180 185 190 Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Met Val Asn Arg Tyr 195 200 205 Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Thr Trp Phe Ser Gln Ser 210 215 220 Gly Lys Ile Gly Met Ala Ser Asp Trp Leu Ile Ser Asp Cys Asn Thr 225 230 235 240 Gly Glu Ile Leu Ile Arg Ala Thr Ser Val Trp Ala Met Met Asn Gln 245 250 255 Lys Thr Arg Arg Phe Ser Arg Leu Pro Tyr Glu Val Arg Gln Glu Leu 260 265 270 Thr Pro His Phe Val Asp Ser Pro His Val Ile Glu Asp Asn Asp Gln 275 280 285 Lys Leu Arg Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Arg Lys Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Ser Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Ile Glu Val Leu 325 330 335 Glu Thr Gln Glu Leu Cys Ser Leu Thr Val Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Met Asp Ser Val Leu Glu Ser Val Thr Ala Val Asp Pro Ser Glu 355 360 365 Asn Gly Gly Arg Ser Gln Tyr Lys His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Thr Asp Ile Val Lys Ser Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Thr Asn Gly Ala Ile Ser Thr Ser Thr Ala Lys Thr Ser Asn Gly Asn 405 410 415 Ser Val Ser 741260DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 74atggtggccg ccgccgccac ctccgccttc ttccccgtgc ccgcccccgg cacctccccc 60aagcccggca agtccggcaa ctggccctcc tccctgtccc ccaccttcaa gcccaagtcc 120atccccaacg gcggcttcca ggtgaaggcc aacgcctccg cccaccccaa ggccaacggc 180tccgccgtga acctgaagtc cggctccctg aacacccagg aggacacctc ctcctccccc 240cccccccgcg ccttcctgaa ccagctgccc gactggtcca tgctgctgac cgccatcacc 300accgtgttcg tggccgccga gaagcagtgg accatgctgg accgcaagtc caagcgcccc 360gacatgctgg tggactccgt gggcctgaag tccatcgtgc gcgacggcct ggtgtcccgc 420cactccttct ccatccgctc ctacgagatc ggcgccgacc gcaccgcctc catcgagacc 480ctgatgaacc acctgcagga gaccaccatc aaccactgca agtccctggg cctgcacaac 540gacggcttcg gccgcacccc cggcatgtgc aagaacgacc tgatctgggt gctgaccaag 600atgcagatca tggtgaaccg ctaccccacc tggggcgaca ccgtggagat caacacctgg 660ttctcccagt ccggcaagat cggcatggcc tccgactggc tgatctccga ctgcaacacc 720ggcgagatcc tgatccgcgc cacctccgtg tgggccatga tgaaccagaa gacccgccgc 780ttctcccgcc tgccctacga ggtgcgccag gagctgaccc cccacttcgt ggactccccc 840cacgtgatcg aggacaacga ccagaagctg cgcaagttcg acgtgaagac cggcgactcc 900atccgcaagg gcctgacccc ccgctggaac gacctggacg tgaaccagca cgtgtccaac 960gtgaagtaca tcggctggat cctggagtcc atgcccatcg aggtgctgga gacccaggag 1020ctgtgctccc tgaccgtgga gtaccgccgc gagtgcggca tggactccgt gctggagtcc 1080gtgaccgccg tggacccctc cgagaacggc ggccgctccc agtacaagca cctgctgcgc 1140ctggaggacg gcaccgacat cgtgaagtcc cgcaccgagt ggcgccccaa gaacgccggc 1200accaacggcg ccatctccac ctccaccgcc aagacctcca acggcaactc cgtgtcctga 126075415PRTCuphea viscosissima 75Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Ala 1 5 10 15 Asp Thr Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Pro Ser Ser Phe 20 25 30 Ser Pro Leu Lys Pro Lys Ser Ile Pro Asn Gly Gly Leu Gln Val Lys 35 40 45 Ala Ser Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly Leu 50 55 60 Lys Ser Gly Gly Leu Lys Thr His Asp Asp Ala Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Ala Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Arg Lys Pro Lys Arg Leu Asp Met Leu Glu Asp Pro Phe Gly Leu 115 120 125 Gly Arg Val Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Thr Ala Gly 165 170 175 Leu Ser Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Lys Leu Ser Lys Ile Pro Asp Glu Val Arg Arg Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Arg Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Ser Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Ala 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Glu 355 360 365 Gly Tyr Gly Ser Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly Ile 385 390 395 400 Asn Gly Val Val Pro Ser Glu Glu Ser Ser Pro Gly Asp Tyr Ser 405 410 415 761248DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 76atggtggcca ccgccgcctc ctccgccttc ttccccgtgc cctccgccga cacctcctcc 60cgccccggca agctgggcaa cggcccctcc tccttctccc ccctgaagcc caagtccatc 120cccaacggcg gcctgcaggt gaaggcctcc gcctccgccc cccccaagat caacggctcc 180tccgtgggcc tgaagtccgg cggcctgaag acccacgacg acgccccctc cgcccccccc 240ccccgcacct tcatcaacca gctgcccgac tggtccatgc tgctggccgc catcaccacc 300gccttcctgg ccgccgagaa gcagtggatg atgctggacc gcaagcccaa gcgcctggac 360atgctggagg accccttcgg cctgggccgc gtggtgcagg acggcctggt gttccgccag 420aacttctcca tccgctccta cgagatcggc gccgaccgca ccgcctccat cgagaccgtg 480atgaaccacc tgcaggagac cgccctgaac cacgtgaaga ccgccggcct gtccaacgac 540ggcttcggcc gcacccccga gatgtacaag cgcgacctga tctgggtggt ggccaagatg 600caggtgatgg tgaaccgcta ccccacctgg ggcgacaccg tggaggtgaa cacctgggtg 660gccaagtccg gcaagaacgg catgcgccgc gactggctga tctccgactg caacaccggc 720gagatcctga cccgcgcctc ctccgtgtgg gtgatgatga accagaagac ccgcaagctg 780tccaagatcc ccgacgaggt gcgccgcgag atcgagcccc acttcgtgga ctccgccccc 840gtgatcgagg acgacgaccg caagctgccc aagctggacg agaagtccgc cgactccatc 900cgcaagggcc tgaccccccg ctggaacgac ctggacgtga accagcacgt gaacaacgcc 960aagtacatcg gctggatcct ggagtccacc ccccccgagg tgctggagac ccaggagctg 1020tgctccctga ccctggagta ccgccgcgag tgcggccgcg agtccgtgct ggagtccctg 1080accgccgtgg acccctccgg cgagggctac ggctcccagt tccagcacct gctgcgcctg 1140gaggacggcg gcgagatcgt gaagggccgc accgagtggc gccccaagaa cgccggcatc 1200aacggcgtgg tgccctccga ggagtcctcc cccggcgact

actcctga 124877412PRTCuphea viscosissima 77Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Phe Pro Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Ile Pro Phe Asn Pro Lys Ser Asn His Asn Gly Gly Ile Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ala Gly Ser Leu Glu Thr Gln Glu Asp Thr Ser Ser Pro Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Ile Ser Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Val Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Val Leu Val Glu Pro Phe 115 120 125 Val Gln Asp Gly Val Ser Phe Arg Gln Ser Phe Ser Ile Arg Ser Tyr 130 135 140 Glu Ile Gly Val Asp Arg Thr Ala Ser Ile Glu Thr Leu Met Asn Ile 145 150 155 160 Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser Leu Gly Leu Leu Asn 165 170 175 Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu Ile Trp 180 185 190 Val Val Thr Lys Met Gln Ile Glu Val Asn Arg Tyr Pro Thr Trp Gly 195 200 205 Asp Thr Ile Glu Val Thr Thr Trp Val Ser Glu Ser Gly Lys Asn Gly 210 215 220 Met Ser Arg Asp Trp Leu Ile Ser Asp Cys His Ser Gly Glu Ile Leu 225 230 235 240 Ile Arg Ala Thr Ser Val Trp Ala Met Met Asn Gln Lys Thr Arg Arg 245 250 255 Leu Ser Lys Ile Pro Asp Glu Val Arg Gln Glu Ile Val Pro Tyr Phe 260 265 270 Val Asp Ser Ala Pro Val Ile Glu Asp Asp Arg Lys Leu His Lys Leu 275 280 285 Asp Val Lys Thr Gly Asp Ser Ile Arg Asn Gly Leu Thr Pro Arg Trp 290 295 300 Asn Asp Phe Asp Val Asn Gln His Val Asn Asn Val Lys Tyr Ile Ala 305 310 315 320 Trp Leu Leu Lys Ser Val Pro Thr Glu Val Phe Glu Thr Gln Glu Leu 325 330 335 Cys Gly Leu Thr Leu Glu Tyr Arg Arg Glu Cys Arg Arg Asp Ser Val 340 345 350 Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys Glu Gly Asp Arg Ser 355 360 365 Leu Tyr Gln His Leu Leu Arg Leu Glu Asn Gly Ala Asp Ile Ala Leu 370 375 380 Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Thr Gly Ala Val 385 390 395 400 Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser Val Ser 405 410 781239DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 78atggtggccg ccgccgcctc ctccgccttc ttctccttcc ccacccccgg cacctccccc 60aagcccggca agttcggcaa ctggccctcc tccctgtcca tccccttcaa ccccaagtcc 120aaccacaacg gcggcatcca ggtgaaggcc aacgcctccg cccaccccaa ggccaacggc 180tccgccgtgt ccctgaaggc cggctccctg gagacccagg aggacacctc ctccccctcc 240cccccccccc gcaccttcat ctcccagctg cccgactggt ccatgctggt gtccgccatc 300accaccgtgt tcgtggccgc cgagaagcag tggaccatgc tggaccgcaa gtccaagcgc 360cccgacgtgc tggtggagcc cttcgtgcag gacggcgtgt ccttccgcca gtccttctcc 420atccgctcct acgagatcgg cgtggaccgc accgcctcca tcgagaccct gatgaacatc 480ttccaggaga cctccctgaa ccactgcaag tccctgggcc tgctgaacga cggcttcggc 540cgcacccccg agatgtgcaa gcgcgacctg atctgggtgg tgaccaagat gcagatcgag 600gtgaaccgct accccacctg gggcgacacc atcgaggtga ccacctgggt gtccgagtcc 660ggcaagaacg gcatgtcccg cgactggctg atctccgact gccactccgg cgagatcctg 720atccgcgcca cctccgtgtg ggccatgatg aaccagaaga cccgccgcct gtccaagatc 780cccgacgagg tgcgccagga gatcgtgccc tacttcgtgg actccgcccc cgtgatcgag 840gacgaccgca agctgcacaa gctggacgtg aagaccggcg actccatccg caacggcctg 900accccccgct ggaacgactt cgacgtgaac cagcacgtga acaacgtgaa gtacatcgcc 960tggctgctga agtccgtgcc caccgaggtg ttcgagaccc aggagctgtg cggcctgacc 1020ctggagtacc gccgcgagtg ccgccgcgac tccgtgctgg agtccgtgac cgccatggac 1080ccctccaagg agggcgaccg ctccctgtac cagcacctgc tgcgcctgga gaacggcgcc 1140gacatcgccc tgggccgcac cgagtggcgc cccaagaacg ccggcgccac cggcgccgtg 1200tccaccggca agacctccaa cggcaactcc gtgtcctga 123979419PRTCuphea sp. 79Met Val Val Ala Ala Ala Thr Ser Ala Phe Phe Pro Val Pro Ala Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Ser Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Pro Thr Phe Lys Pro Lys Ser Ile Pro Asn Ala Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn 50 55 60 Leu Lys Ser Gly Ser Leu Asn Thr Gln Glu Asp Thr Ser Ser Ser Pro 65 70 75 80 Pro Pro Arg Ala Phe Leu Asn Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Thr Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr Met 100 105 110 Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Ser Val Gly 115 120 125 Leu Lys Ser Ile Val Arg Asp Gly Leu Val Ser Arg Gln Ser Phe Leu 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Leu Met Asn His Leu Gln Glu Thr Ser Ile Asn His Cys Lys Ser Leu 165 170 175 Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Gly Met Cys Lys Asn 180 185 190 Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Met Val Asn Arg Tyr 195 200 205 Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Thr Trp Phe Ser Gln Ser 210 215 220 Gly Lys Ile Gly Met Ala Ser Asp Trp Leu Ile Ser Asp Cys Asn Thr 225 230 235 240 Gly Glu Ile Leu Ile Arg Ala Thr Ser Val Trp Ala Met Met Asn Gln 245 250 255 Lys Thr Arg Arg Phe Ser Arg Leu Pro Tyr Glu Val Arg Gln Glu Leu 260 265 270 Thr Pro His Phe Val Asp Ser Pro His Val Ile Glu Asp Asn Asp Gln 275 280 285 Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Arg Lys Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Ser Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Met Pro Ile Glu Val Leu 325 330 335 Glu Thr Gln Glu Leu Cys Ser Leu Thr Val Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Met Asp Ser Val Leu Glu Ser Val Thr Ala Val Asp Pro Ser Glu 355 360 365 Asn Gly Gly Arg Ser Gln Tyr Lys His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Thr Asp Ile Val Lys Ser Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Thr Asn Gly Ala Ile Ser Thr Ser Thr Ala Lys Thr Ser Asn Gly Asn 405 410 415 Ser Ala Ser 80354PRTCuphea wrightii 80Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Pro Arg Thr Thr Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser 20 25 30 Leu Ser Pro Pro Phe Lys Pro Lys Ser Asn Pro Asn Gly Arg Phe Gln 35 40 45 Val Lys Ala Asn Val Ser Pro His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Leu Glu Asp Leu Pro Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Arg Thr Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Phe Thr 100 105 110 Arg Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Trp Phe 115 120 125 Gly Ser Glu Thr Ile Val Gln Asp Gly Leu Val Phe Arg Glu Arg Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn His Leu Gln Asp Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Val Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Ser Glu Met Cys Thr 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Val Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Val Arg Ala Thr Ser Ala Trp Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Leu Pro Cys Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Cys Lys 290 295 300 Gly Leu Thr Pro Gly Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Lys Phe Trp Arg Pro Arg 325 330 335 Ser Tyr Ala Leu Ser Pro Leu Asn Ile Gly Gly Asn Val Glu Gly Lys 340 345 350 Val Trp 81354PRTCuphea wrightii 81Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Pro Arg Thr Thr Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser 20 25 30 Leu Ser Pro Pro Phe Lys Pro Lys Ser Asn Pro Asn Gly Arg Phe Gln 35 40 45 Val Lys Ala Asn Val Ser Pro His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Leu Glu Asp Leu Pro Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Arg Thr Ala Ile Thr Thr Val Phe Val Ala Thr Glu Lys Gln Phe Thr 100 105 110 Arg Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Trp Phe 115 120 125 Gly Ser Glu Thr Ile Val Gln Asp Gly Leu Val Phe Arg Glu Arg Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn His Leu Gln Asp Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Val Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Ser Glu Met Cys Thr 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Val Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Val Arg Ala Thr Ser Ala Trp Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Leu Pro Cys Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Cys Lys 290 295 300 Gly Leu Thr Pro Gly Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Lys Phe Trp Arg Pro Arg 325 330 335 Ser Tyr Ala Leu Ser Pro Leu Asn Ile Gly Gly Asn Val Glu Gly Lys 340 345 350 Val Trp 82416PRTCuphea wrightii 82Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Ala 1 5 10 15 Asp Thr Ser Ser Ser Arg Pro Gly Lys Leu Gly Ser Gly Pro Ser Ser 20 25 30 Leu Ser Pro Leu Lys Pro Lys Ser Ile Pro Asn Gly Gly Leu Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly 50 55 60 Leu Lys Ser Gly Gly Phe Lys Thr Gln Glu Asp Ser Pro Ser Ala Pro 65 70 75 80 Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Ala Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met 100 105 110 Leu Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly 115 120 125 Leu Gly Ser Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Val Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ile Ala 165 170 175 Gly Leu Ser Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg 180 185 190 Asp Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr 195 200 205 Pro Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser 210 215 220 Gly Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr 225 230 235 240 Gly Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln 245 250 255 Lys Thr Arg Arg Leu Ser Lys Ile Pro Asp Glu Val Arg Asn Glu Ile 260 265 270 Glu Pro His Phe Val Asp Ser Ala Pro Val Val Glu Asp Asp Asp Arg 275 280 285 Lys Leu Pro Lys Leu Asp Glu Asn Thr Ala Asp Ser Ile Arg Lys Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu 325 330 335 Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Ala 355 360 365 Glu Gly Tyr Ala Ser Arg Phe Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Gly Glu Ile Val Lys Ala Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Ile Asn Trp Val Val Pro Ser Glu Glu Ser Ser Pro Gly Asp Phe Phe 405 410 415 83416PRTCuphea wrightii 83Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Ala 1 5 10 15 Asp Thr Ser Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Pro Ser Ser 20 25 30 Leu Ser Pro Leu Lys Pro Lys Ser Ile Pro Asn Gly Gly Leu Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly 50 55 60 Leu Lys Ser Gly Ser Phe Lys Thr Gln Glu Asp Ala Pro Ser Ala Pro 65 70 75 80 Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Ala Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met 100 105 110 Leu Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly 115 120 125 Leu

Gly Ser Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Val Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ile Ala 165 170 175 Gly Leu Ser Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg 180 185 190 Asp Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr 195 200 205 Pro Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser 210 215 220 Gly Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr 225 230 235 240 Gly Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln 245 250 255 Lys Thr Arg Arg Leu Ser Lys Ile Pro Asp Glu Val Arg Asn Glu Ile 260 265 270 Glu Pro His Phe Val Asp Ser Ala Pro Val Val Glu Asp Asp Asp Arg 275 280 285 Lys Leu Pro Lys Leu Asp Glu Asn Thr Ala Asp Ser Ile Arg Lys Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu 325 330 335 Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Ala 355 360 365 Glu Gly Tyr Ala Ser Arg Phe Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Gly Glu Ile Val Lys Ala Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Ile Asn Gly Val Val Pro Ser Glu Glu Ser Ser Pro Gly Asp Phe Phe 405 410 415 84416PRTCuphea wrightii 84Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Ala 1 5 10 15 Asp Thr Ser Ser Ser Arg Pro Gly Lys Leu Gly Ser Gly Pro Ser Ser 20 25 30 Leu Ser Pro Leu Lys Pro Lys Ser Ile Pro Asn Gly Gly Leu Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly 50 55 60 Leu Lys Ser Gly Gly Phe Lys Thr Gln Glu Asp Ser Pro Ser Ala Pro 65 70 75 80 Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Ala Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met 100 105 110 Leu Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly 115 120 125 Leu Gly Ser Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Val Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ile Ala 165 170 175 Gly Leu Ser Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg 180 185 190 Asp Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr 195 200 205 Pro Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser 210 215 220 Gly Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr 225 230 235 240 Gly Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln 245 250 255 Lys Thr Arg Arg Leu Ser Lys Ile Pro Asp Glu Val Arg Asn Glu Ile 260 265 270 Glu Pro His Phe Val Asp Ser Ala Pro Val Val Glu Asp Asp Asp Arg 275 280 285 Lys Leu Pro Lys Leu Asp Glu Asn Thr Ala Asp Ser Ile Arg Lys Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu 325 330 335 Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Ala 355 360 365 Glu Gly Tyr Val Ser Arg Phe Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Gly Glu Ile Val Lys Ala Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Ile Asn Gly Val Val Pro Ser Glu Glu Ser Ser Pro Gly Asp Phe Phe 405 410 415 85417PRTCuphea wrightii 85Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Ala 1 5 10 15 Asp Thr Ser Ser Ser Arg Pro Gly Lys Leu Gly Ser Gly Pro Ser Ser 20 25 30 Leu Ser Pro Leu Lys Pro Lys Ser Ile Pro Asn Gly Gly Leu Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly 50 55 60 Leu Lys Ser Gly Ser Phe Lys Thr Gln Glu Asp Ala Pro Ser Ala Pro 65 70 75 80 Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Ala Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met 100 105 110 Leu Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly 115 120 125 Leu Gly Ser Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Val Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ile Ala 165 170 175 Gly Leu Ser Ser Asp Gly Phe Gly Arg Thr Pro Ala Met Ser Lys Arg 180 185 190 Asp Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr 195 200 205 Pro Ala Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser 210 215 220 Gly Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr 225 230 235 240 Gly Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln 245 250 255 Lys Thr Arg Arg Leu Ser Lys Ile Pro Asp Glu Val Arg Asn Glu Ile 260 265 270 Glu Pro His Phe Val Asp Ser Ala Pro Val Val Glu Asp Asp Asp Arg 275 280 285 Lys Leu Pro Lys Leu Asp Glu Asn Thr Ala Asp Ser Ile Arg Lys Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Ala Glu Val Leu 325 330 335 Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly 355 360 365 Glu Gly Asp Gly Ser Lys Phe Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Gly Glu Ile Val Lys Ala Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Ile Asn Gly Val Val Pro Ser Glu Glu Ser Ser Pro Gly Gly Asp Phe 405 410 415 Phe 86413PRTCuphea wrightii 86Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Pro Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Asp Asn Gly Gly Phe His Val Lys Ala Asn 35 40 45 Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn Leu Lys Ser 50 55 60 Gly Ser Leu Glu Thr Pro Pro Arg Ser Phe Ile Asn Gln Leu Pro Asp 65 70 75 80 Leu Ser Val Leu Leu Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu 85 90 95 Lys Gln Trp Lys Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp 100 105 110 Arg Ile Phe Gln Asp Gly Phe Phe Phe Arg Gln Ser Phe Ser Ile Arg 115 120 125 Ser Tyr Glu Ile Gly Val Asp Arg Thr Ala Ser Ile Glu Thr Leu Met 130 135 140 Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu 145 150 155 160 Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu 165 170 175 Ile Trp Val Val Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr 180 185 190 Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys 195 200 205 Asn Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu 210 215 220 Ile Leu Ile Arg Ala Thr Ser Val Trp Ala Met Met Asn Gln Asn Thr 225 230 235 240 Arg Arg Leu Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro 245 250 255 His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Gln Lys Leu Gln 260 265 270 Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Arg Asp Gly Leu Thr Pro 275 280 285 Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val Lys Tyr 290 295 300 Ile Gly Trp Ile Leu Lys Ser Val Pro Ile Glu Val Phe Glu Thr Gln 305 310 315 320 Glu Leu Cys Gly Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp 325 330 335 Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ala Lys Glu Gly Asp 340 345 350 Arg Cys Val Tyr Gln His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile 355 360 365 Thr Ile Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Gly 370 375 380 Ala Met Ser Ser Gly Lys Thr Ser Asn Gly Asn Cys Leu Ile Glu Gly 385 390 395 400 Arg Gly Trp Gln Pro Phe Arg Val Val Arg Leu Ile Phe 405 410 87413PRTCuphea wrightii 87Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Pro Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Asp Asn Gly Gly Phe His Val Lys Ala Asn 35 40 45 Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn Leu Lys Ser 50 55 60 Gly Ser Leu Glu Thr Pro Pro Arg Ser Phe Ile Asn Gln Leu Pro Asp 65 70 75 80 Leu Ser Val Leu Leu Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu 85 90 95 Lys Gln Trp Lys Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp 100 105 110 Arg Ile Phe Gln Asp Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg 115 120 125 Ser Tyr Glu Ile Gly Val Asp Arg Thr Ala Ser Ile Glu Thr Leu Met 130 135 140 Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu 145 150 155 160 Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu 165 170 175 Ile Trp Val Val Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr 180 185 190 Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys 195 200 205 Asn Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu 210 215 220 Ile Leu Ile Arg Ala Thr Ser Val Trp Ala Met Met Asn Gln Asn Thr 225 230 235 240 Arg Arg Leu Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro 245 250 255 His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Gln Lys Leu Gln 260 265 270 Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Arg Asp Gly Leu Thr Pro 275 280 285 Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val Lys Tyr 290 295 300 Ile Gly Trp Ile Leu Lys Ser Val Pro Ile Glu Val Phe Glu Thr Gln 305 310 315 320 Glu Leu Cys Gly Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp 325 330 335 Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ala Lys Glu Gly Asp 340 345 350 Arg Cys Val Tyr Gln His Leu Leu Trp Leu Glu Asp Gly Ala Asp Ile 355 360 365 Thr Ile Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Gly 370 375 380 Ala Met Ser Ser Gly Lys Thr Ser Asn Gly Asn Cys Leu Ile Glu Gly 385 390 395 400 Arg Gly Trp Gln Pro Phe Arg Val Val Arg Leu Ile Phe 405 410 88413PRTCuphea wrightii 88Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Pro Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Asp Asn Gly Gly Phe His Val Lys Ala Asn 35 40 45 Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn Leu Lys Ser 50 55 60 Gly Ser Leu Glu Thr Pro Pro Arg Ser Phe Ile Asn Gln Leu Pro Asp 65 70 75 80 Leu Ser Val Leu Leu Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu 85 90 95 Lys Gln Trp Lys Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp 100 105 110 Arg Ile Phe Gln Asp Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg 115 120 125 Ser Tyr Glu Ile Gly Val Asp Arg Thr Ala Ser Ile Glu Thr Leu Met 130 135 140 Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu 145 150 155 160 Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu 165 170 175 Ile Trp Val Val Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Ile 180 185 190 Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys 195 200 205 Asn Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu 210 215 220 Ile Leu Ile Arg Ala Thr Ser Val Trp Ala Met Met Asn Gln Asn Thr 225 230 235 240 Arg Arg Leu Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro 245 250 255 His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Gln Lys Leu Gln 260 265 270 Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Arg Asp Gly Leu Thr Pro 275 280 285 Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val Lys Tyr 290 295 300 Ile Gly Trp Ile Leu Lys Ser Val Pro Ile Glu Val Phe Glu Thr Gln 305 310 315 320 Glu Leu Cys Gly Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp 325 330 335 Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ala Lys Glu Gly Asp

340 345 350 Arg Cys Val Tyr Gln His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile 355 360 365 Thr Ile Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Gly 370 375 380 Ala Met Ser Ser Gly Lys Thr Ser Asn Gly Asn Cys Leu Ile Glu Gly 385 390 395 400 Met Gly Trp Gln Pro Phe Arg Val Val Arg Leu Ile Phe 405 410 89409PRTCuphea wrightii 89Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Arg Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Glu Thr Asn His Asn Gly Gly Phe His Ile 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Leu Asn 50 55 60 Leu Lys Ser Gly Ser Leu Glu Thr Gln Glu Asp Thr Ser Leu Ser Ser 65 70 75 80 Pro Pro Arg Thr Phe Ile Lys Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu Lys Gln Leu Lys Arg 100 105 110 Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp Arg Ile Phe Gln Asp 115 120 125 Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg Ser Tyr Glu Ile Gly 130 135 140 Ala Asp Arg Thr Ala Ser Ile Glu Thr Leu Met Asn Ile Phe Gln Glu 145 150 155 160 Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu Leu Asn Asp Gly Phe 165 170 175 Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu Ile Trp Val Val Thr 180 185 190 Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr Trp Gly Asp Thr Ile 195 200 205 Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys Asn Gly Met Gly Arg 210 215 220 Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu Ile Leu Ile Arg Ala 225 230 235 240 Thr Ser Val Trp Ala Met Met Asn Gln Asn Thr Arg Arg Leu Ser Lys 245 250 255 Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro His Phe Val Asp Ser 260 265 270 Ala Pro Val Ile Glu Asp Asp Arg Lys Leu Tyr Lys Leu Asn Val Lys 275 280 285 Thr Gly Asp Ser Ile Arg Asp Gly Leu Thr Pro Arg Trp Asn Asp Leu 290 295 300 Asp Val Asn Gln His Val Asn Asn Val Lys Phe Ile Gly Trp Ile Leu 305 310 315 320 Lys Ser Val Pro Thr Lys Val Phe Glu Thr Gln Glu Leu Cys Gly Val 325 330 335 Thr Leu Glu Tyr Arg Arg Glu Cys Gly Lys Asp Ser Val Leu Glu Ser 340 345 350 Val Thr Ala Met Asp Pro Ala Lys Glu Gly Asp Arg Ser Val Tyr Gln 355 360 365 His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile Thr Ile Gly Arg Thr 370 375 380 Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Glu Ala Ile Ser Ser Gly 385 390 395 400 Lys Thr Ser Asn Gly Asn Ser Ala Ser 405 90409PRTCuphea wrightii 90Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Arg Asn Trp Pro Leu Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Glu Thr Asn His Asn Gly Gly Phe His Ile 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Leu Asn 50 55 60 Leu Lys Ser Gly Ser Leu Glu Thr Gln Glu Asp Thr Ser Leu Ser Ser 65 70 75 80 Pro Pro Arg Thr Phe Ile Lys Gln Leu Pro Asp Trp Ser Met Leu Leu 85 90 95 Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu Lys Gln Leu Lys Arg 100 105 110 Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp Arg Ile Phe Gln Asp 115 120 125 Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg Ser Tyr Glu Ile Gly 130 135 140 Ala Asp Arg Thr Ala Ser Ile Glu Thr Leu Met Asn Ile Phe Gln Glu 145 150 155 160 Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu Leu Asn Asp Gly Phe 165 170 175 Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu Ile Trp Val Val Thr 180 185 190 Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr Trp Gly Asp Thr Ile 195 200 205 Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys Asn Gly Met Gly Arg 210 215 220 Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu Ile Leu Ile Arg Ala 225 230 235 240 Thr Ser Val Trp Ala Met Met Asn Gln Asn Thr Arg Arg Leu Ser Lys 245 250 255 Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro His Phe Val Asp Ser 260 265 270 Ala Pro Val Ile Glu Asp Asp Arg Lys Leu Tyr Lys Leu Asn Val Lys 275 280 285 Thr Gly Asp Ser Ile Arg Asp Gly Leu Thr Pro Arg Trp Asn Asp Leu 290 295 300 Asp Val Asn Gln His Val Asn Asn Val Lys Phe Ile Gly Trp Ile Leu 305 310 315 320 Lys Ser Val Pro Thr Lys Val Phe Glu Thr Gln Glu Leu Cys Gly Val 325 330 335 Thr Leu Glu Tyr Arg Arg Glu Cys Gly Lys Asp Ser Val Leu Glu Ser 340 345 350 Val Thr Ala Met Asp Pro Ala Lys Glu Gly Asp Arg Ser Val Tyr Gln 355 360 365 His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile Thr Ile Gly Arg Thr 370 375 380 Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Glu Ala Ile Ser Ser Gly 385 390 395 400 Lys Thr Ser Asn Gly Asn Ser Ala Ser 405 91357PRTCinnamomum camphora 91Met Val Thr Thr Ser Leu Ala Ser Ala Tyr Phe Ser Met Lys Ala Val 1 5 10 15 Met Leu Ala Pro Asp Gly Arg Gly Ile Lys Pro Arg Ser Ser Gly Leu 20 25 30 Gln Val Arg Ala Gly Asn Glu Arg Asn Ser Cys Lys Val Ile Asn Gly 35 40 45 Thr Lys Val Lys Asp Thr Glu Gly Leu Lys Gly Cys Ser Thr Leu Gln 50 55 60 Gly Gln Ser Met Leu Asp Asp His Phe Gly Leu His Gly Leu Val Phe 65 70 75 80 Arg Arg Thr Phe Ala Ile Arg Cys Tyr Glu Val Gly Pro Asp Arg Ser 85 90 95 Thr Ser Ile Met Ala Val Met Asn His Leu Gln Glu Ala Ala Arg Asn 100 105 110 His Ala Glu Ser Leu Gly Leu Leu Gly Asp Gly Phe Gly Glu Thr Leu 115 120 125 Glu Met Ser Lys Arg Asp Leu Ile Trp Val Val Arg Arg Thr His Val 130 135 140 Ala Val Glu Arg Tyr Pro Ala Trp Gly Asp Thr Val Glu Val Glu Ala 145 150 155 160 Trp Val Gly Ala Ser Gly Asn Thr Gly Met Arg Arg Asp Phe Leu Val 165 170 175 Arg Asp Cys Lys Thr Gly His Ile Leu Thr Arg Cys Thr Ser Val Ser 180 185 190 Val Met Met Asn Met Arg Thr Arg Arg Leu Ser Lys Ile Pro Gln Glu 195 200 205 Val Arg Ala Glu Ile Asp Pro Leu Phe Ile Glu Lys Val Ala Val Lys 210 215 220 Glu Gly Glu Ile Lys Lys Leu Gln Lys Leu Asn Asp Ser Thr Ala Asp 225 230 235 240 Tyr Ile Gln Gly Gly Trp Thr Pro Arg Trp Asn Asp Leu Asp Val Asn 245 250 255 Gln His Val Asn Asn Ile Ile Tyr Val Gly Trp Ile Phe Lys Ser Val 260 265 270 Pro Asp Ser Ile Ser Glu Asn His His Leu Ser Ser Ile Thr Leu Glu 275 280 285 Tyr Arg Arg Glu Cys Ile Arg Gly Asn Lys Leu Gln Ser Leu Thr Thr 290 295 300 Val Cys Gly Gly Ser Ser Glu Ala Gly Ile Ile Cys Glu His Leu Leu 305 310 315 320 Gln Leu Glu Asp Gly Ser Glu Val Leu Arg Ala Arg Thr Glu Trp Arg 325 330 335 Pro Lys His Thr Asp Ser Phe Gln Gly Ile Ser Glu Arg Phe Pro Gln 340 345 350 Gln Glu Pro His Lys 355 92419PRTCinnamomum camphora 92Met Val Ala Thr Ala Ala Ala Ser Ala Phe Phe Pro Val Gly Ala Pro 1 5 10 15 Ala Thr Ser Ser Ala Thr Ser Ala Lys Ala Ser Met Met Pro Asp Asn 20 25 30 Leu Asp Ala Arg Gly Ile Lys Pro Lys Leu Ala Ser Ser Ser Gly Leu 35 40 45 Gln Val Lys Ala Asn Ala His Ala Ser Pro Lys Ile Asn Gly Ser Lys 50 55 60 Val Ser Thr Asp Thr Leu Lys Gly Glu Asp Thr Leu Thr Ser Ser Pro 65 70 75 80 Ala Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Phe Leu 85 90 95 Ala Ala Ile Thr Thr Ile Phe Leu Ala Ala Glu Lys Gln Trp Thr Asn 100 105 110 Leu Asp Trp Lys Pro Arg Arg Pro Asp Met Leu Ala Asp Pro Phe Gly 115 120 125 Ile Gly Arg Phe Met Gln Asp Gly Leu Ile Phe Arg Gln His Phe Ala 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Leu Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Arg Ser Ala 165 170 175 Gly Leu Leu Gly Asp Gly Phe Gly Ala Thr Pro Glu Met Ser Arg Arg 180 185 190 Asp Leu Ile Trp Val Val Thr Arg Met Gln Val Leu Val Asp Arg Tyr 195 200 205 Pro Ala Trp Gly Asp Ile Val Glu Val Glu Thr Trp Val Gly Ala Ser 210 215 220 Gly Lys Asn Gly Met Arg Arg Asp Trp Leu Val Arg Asp Ser Gln Thr 225 230 235 240 Gly Glu Ile Leu Thr Arg Ala Thr Ser Val Trp Val Met Met Asn Lys 245 250 255 Arg Thr Arg Arg Leu Ser Lys Leu Pro Glu Glu Val Arg Gly Glu Ile 260 265 270 Gly Pro Tyr Phe Ile Glu Asp Val Ala Ile Ile Glu Glu Asp Asn Arg 275 280 285 Lys Leu Gln Lys Leu Asn Glu Asn Thr Ala Asp Asn Val Arg Arg Gly 290 295 300 Leu Thr Pro Arg Trp Ser Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Ala Pro Gly Ser Ile Leu 325 330 335 Glu Ser His Glu Leu Ser Cys Met Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Lys Asp Ser Val Leu Gln Ser Met Thr Ala Val Ser Gly Gly Gly 355 360 365 Ser Ala Ala Gly Gly Ser Pro Glu Ser Ser Val Glu Cys Asp His Leu 370 375 380 Leu Gln Leu Glu Ser Gly Pro Glu Val Val Arg Gly Arg Thr Glu Trp 385 390 395 400 Arg Pro Lys Ser Ala Asn Asn Ser Arg Ser Ile Leu Glu Met Pro Ala 405 410 415 Glu Ser Leu 93419PRTCinnamomum camphora 93Met Val Ala Thr Ala Ala Ala Ser Ala Phe Phe Pro Val Gly Ala Pro 1 5 10 15 Ala Thr Ser Ser Ala Thr Ser Ala Lys Ala Ser Met Met Pro Asp Asn 20 25 30 Leu Asp Ala Arg Gly Ile Lys Pro Lys Pro Ala Ser Ser Ser Gly Leu 35 40 45 Gln Val Lys Ala Asn Ala His Ala Ser Pro Lys Ile Asn Gly Ser Lys 50 55 60 Val Ser Thr Asp Thr Leu Lys Gly Glu Asp Thr Leu Thr Ser Ser Pro 65 70 75 80 Ala Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Phe Leu 85 90 95 Ala Ala Ile Thr Thr Ile Phe Leu Ala Ala Glu Lys Gln Trp Thr Asn 100 105 110 Leu Asp Trp Lys Pro Arg Arg Pro Asp Met Leu Ala Asp Pro Phe Gly 115 120 125 Ile Gly Arg Phe Met Gln Asp Gly Leu Ile Phe Arg Gln His Phe Ala 130 135 140 Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr 145 150 155 160 Leu Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Arg Ser Ala 165 170 175 Gly Leu Leu Gly Asp Gly Phe Gly Ala Thr Pro Glu Met Ser Arg Arg 180 185 190 Asp Leu Ile Trp Val Val Thr Arg Met Gln Val Leu Val Asp Arg Tyr 195 200 205 Pro Ala Trp Gly Asp Ile Val Glu Val Glu Thr Trp Val Gly Ala Ser 210 215 220 Gly Lys Asn Gly Met Arg Arg Asp Trp Leu Val Arg Asp Ser Gln Thr 225 230 235 240 Gly Glu Ile Leu Thr Arg Ala Thr Ser Val Trp Val Met Met Asn Lys 245 250 255 Arg Thr Arg Arg Leu Ser Lys Leu Pro Glu Glu Val Arg Gly Glu Ile 260 265 270 Gly Pro Tyr Phe Ile Glu Asp Val Ala Ile Ile Glu Glu Asp Asn Arg 275 280 285 Lys Leu Gln Lys Leu Asn Glu Asn Thr Ala Asp Asn Val Arg Arg Gly 290 295 300 Leu Thr Pro Arg Trp Ser Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Ala Lys Tyr Ile Gly Trp Ile Leu Glu Ser Ala Pro Gly Ser Ile Leu 325 330 335 Glu Ser His Glu Leu Ser Cys Met Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Lys Asp Ser Val Leu Gln Ser Met Thr Ala Val Ser Gly Gly Gly 355 360 365 Ser Ala Ala Gly Gly Ser Pro Glu Ser Ser Val Glu Cys Asp His Leu 370 375 380 Leu Gln Leu Glu Ser Gly Pro Glu Val Val Arg Gly Arg Thr Glu Trp 385 390 395 400 Arg Pro Lys Ser Ala Asn Asn Ser Arg Ser Ile Leu Glu Met Pro Ala 405 410 415 Glu Ser Leu 94410PRTCuphea heterophylla 94Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Asn Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Glu Ser Asn His Asn Gly Gly Phe Arg Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn 50 55 60 Leu Lys Ser Gly Ser Leu Glu Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Ile Lys Gln Leu Pro Asp Trp Gly Met Leu 85 90 95 Leu Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu Arg Gln Trp Lys 100 105 110 Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp Arg Ile Phe Gln 115 120 125 Asp Gly Val Phe Phe Arg His Ser Phe Ser Ile Arg Ser Tyr Glu Ile 130 135 140 Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Leu Met Asn Ile Phe Gln 145 150 155 160 Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu Leu Asn Asp Gly 165 170 175 Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu Ile Trp Val Val 180 185 190 Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr Trp Gly Asp Thr 195 200 205

Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys Asn Gly Met Gly 210 215 220 Arg Asp Trp Leu Ile Gly Asp Cys Arg Thr Gly Glu Ile Leu Ile Arg 225 230 235 240 Ala Thr Ser Val Trp Ala Met Met Asn Arg Lys Thr Arg Arg Leu Ser 245 250 255 Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro His Phe Val Asp 260 265 270 Ser Ala Pro Val Ile Glu Asp Asp Lys Lys Leu His Lys Leu Asp Val 275 280 285 Lys Thr Gly Asp Ser Ile Arg Lys Gly Leu Thr Pro Arg Trp Asn Asp 290 295 300 Leu Asp Val Asn Gln His Val Asn Asn Val Lys Tyr Ile Gly Trp Ile 305 310 315 320 Leu Lys Ser Val Pro Ala Glu Val Phe Glu Thr Gln Glu Leu Cys Gly 325 330 335 Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp Ser Val Leu Glu 340 345 350 Ser Val Thr Ala Met Asp Thr Ala Lys Glu Gly Asp Arg Ser Leu Tyr 355 360 365 Gln His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile Thr Ile Gly Arg 370 375 380 Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Gly Ala Leu Ser Thr 385 390 395 400 Gly Lys Thr Ser Asn Gly Asn Ser Val Ser 405 410 95410PRTCuphea heterophylla 95Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Asn Phe Gly Asn Trp Pro Ser Asn Leu 20 25 30 Ser Val Pro Phe Lys Pro Glu Ser Asn His Asn Gly Gly Phe Arg Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn 50 55 60 Leu Lys Ser Gly Ser Leu Glu Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Ile Lys Gln Leu Pro Asp Trp Gly Met Leu 85 90 95 Leu Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu Arg Gln Trp Lys 100 105 110 Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp Arg Ile Phe Gln 115 120 125 Asp Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg Ser Tyr Glu Ile 130 135 140 Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Leu Met Asn Ile Phe Gln 145 150 155 160 Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu Leu Asn Asp Gly 165 170 175 Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu Ile Trp Val Val 180 185 190 Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr Trp Gly Asp Thr 195 200 205 Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys Asn Gly Met Gly 210 215 220 Arg Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu Ile Leu Ile Arg 225 230 235 240 Ala Thr Ser Val Trp Ala Met Met Asn Arg Lys Thr Arg Arg Leu Ser 245 250 255 Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro His Phe Val Asp 260 265 270 Ser Ala Pro Val Ile Glu Asp Asp Lys Lys Leu His Lys Leu Asp Val 275 280 285 Lys Thr Gly Asp Ser Ile Arg Lys Gly Leu Thr Pro Arg Trp Asn Asp 290 295 300 Phe Asp Val Asn Gln His Val Asn Asn Val Lys Tyr Ile Gly Trp Ile 305 310 315 320 Leu Lys Ser Val Pro Ala Glu Val Phe Glu Thr Gln Glu Leu Cys Gly 325 330 335 Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp Ser Val Leu Glu 340 345 350 Ser Val Thr Ala Met Asp Thr Ala Lys Glu Gly Asp Arg Ser Leu Tyr 355 360 365 Gln His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile Thr Ile Gly Arg 370 375 380 Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Gly Ala Ile Ser Thr 385 390 395 400 Gly Lys Thr Ser Asn Glu Asn Ser Val Ser 405 410 96410PRTCuphea heterophylla 96Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Asn Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Glu Ser Asn His Asn Gly Gly Phe Arg Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn 50 55 60 Leu Lys Ser Gly Ser Leu Glu Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Ile Lys Gln Leu Pro Asp Trp Gly Met Leu 85 90 95 Leu Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu Arg Gln Trp Lys 100 105 110 Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp Arg Ile Phe Gln 115 120 125 Asp Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg Ser Tyr Glu Ile 130 135 140 Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Leu Met Asn Ile Phe Gln 145 150 155 160 Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu Leu Asn Asp Gly 165 170 175 Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu Ile Trp Val Val 180 185 190 Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr Trp Gly Asp Thr 195 200 205 Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys Asn Gly Met Gly 210 215 220 Arg Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu Ile Leu Ile Arg 225 230 235 240 Ala Thr Ser Val Trp Ala Met Met Asn Arg Lys Thr Arg Arg Leu Ser 245 250 255 Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro His Phe Val Asp 260 265 270 Ser Ala Pro Val Ile Glu Asp Asp Lys Lys Leu His Lys Leu Asp Val 275 280 285 Lys Thr Gly Asp Ser Ile Arg Lys Gly Leu Thr Pro Arg Trp Asn Asp 290 295 300 Phe Asp Val Asn Gln His Val Asn Asn Val Lys Tyr Ile Gly Trp Ile 305 310 315 320 Leu Lys Ser Val Pro Ala Glu Val Phe Glu Thr Gln Glu Leu Cys Gly 325 330 335 Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp Ser Val Leu Glu 340 345 350 Ser Val Thr Ala Met Asp Thr Ala Lys Glu Gly Asp Arg Ser Leu Tyr 355 360 365 Gln His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile Thr Ile Gly Arg 370 375 380 Thr Glu Trp Arg Pro Lys Asn Ala Gly Val Asn Gly Ala Ile Ser Thr 385 390 395 400 Gly Lys Thr Ser Asn Glu Asn Ser Val Ser 405 410 97410PRTCuphea heterophylla 97Met Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Ser Val Pro Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Asn Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Pro Glu Ser Asn His Asn Gly Gly Phe Arg Val 35 40 45 Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val Asn 50 55 60 Leu Lys Ser Gly Ser Leu Glu Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Ile Lys Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Lys Ile Thr Thr Val Phe Gly Ala Ala Glu Arg Gln Trp Lys 100 105 110 Arg Pro Gly Met Leu Val Glu Pro Phe Gly Val Asp Arg Ile Phe Gln 115 120 125 Asp Gly Val Phe Phe Arg Gln Ser Phe Ser Ile Arg Ser Tyr Glu Ile 130 135 140 Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Leu Met Asn Ile Phe Gln 145 150 155 160 Glu Thr Ser Leu Asn His Cys Lys Ser Ile Gly Leu Leu Asn Asp Gly 165 170 175 Phe Gly Arg Thr Pro Glu Met Cys Lys Arg Asp Leu Ile Trp Val Val 180 185 190 Thr Lys Ile Gln Val Glu Val Asn Arg Tyr Pro Thr Trp Gly Asp Thr 195 200 205 Ile Glu Val Asn Thr Trp Val Ser Glu Ser Gly Lys Asn Gly Met Gly 210 215 220 Arg Asp Trp Leu Ile Ser Asp Cys Arg Thr Gly Glu Ile Leu Ile Arg 225 230 235 240 Ala Thr Ser Val Trp Ala Met Met Asn Arg Lys Thr Arg Arg Leu Ser 245 250 255 Lys Phe Pro Tyr Glu Val Arg Gln Glu Ile Ala Pro His Phe Val Asp 260 265 270 Ser Ala Pro Val Ile Glu Asp Asp Lys Lys Leu His Lys Leu Asp Val 275 280 285 Lys Thr Gly Asp Ser Ile Arg Lys Gly Leu Thr Pro Arg Trp Asn Asp 290 295 300 Phe Asp Val Asn Gln His Val Asn Asn Val Lys Tyr Ile Gly Trp Ile 305 310 315 320 Leu Lys Ser Val Pro Ala Glu Val Phe Glu Thr Gln Glu Leu Cys Gly 325 330 335 Val Thr Leu Glu Tyr Arg Arg Glu Cys Gly Arg Asp Ser Val Leu Glu 340 345 350 Ser Val Thr Ala Met Asp Thr Ala Lys Glu Gly Asp Arg Ser Leu Tyr 355 360 365 Gln His Leu Leu Arg Leu Glu Asp Gly Ala Asp Ile Thr Ile Gly Arg 370 375 380 Thr Glu Trp Arg Pro Lys Asn Ala Gly Ala Asn Gly Ala Ile Ser Thr 385 390 395 400 Gly Lys Thr Ser Asn Glu Asn Ser Val Ser 405 410 98418PRTCuphea heterophylla 98Met Val Val Ala Ala Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ala 1 5 10 15 Ser Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Thr Trp Leu Ser Ser 20 25 30 Ser Ser Pro Ser Tyr Lys Pro Lys Ser Asn Pro Ser Gly Gly Phe Gln 35 40 45 Val Lys Ala Asn Ala Ser Ala His Pro Lys Ala Asn Gly Ser Ala Val 50 55 60 Ser Leu Lys Ser Gly Ser Leu Asn Thr Gln Glu Gly Thr Ser Ser Ser 65 70 75 80 Pro Pro Pro Arg Thr Phe Leu Asn Gln Leu Pro Asp Trp Ser Arg Leu 85 90 95 Leu Thr Ala Ile Ser Thr Val Phe Val Ala Ala Glu Lys Gln Leu Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Val Asp Trp Phe 115 120 125 Gly Leu Glu Ser Ile Val Gln Asp Gly Leu Val Phe Arg Glu Ser Tyr 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Ser Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Val Met Asn Leu Leu Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Met Gly Ile Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Leu Thr Lys Met Gln Ile Leu Val Asn Arg 195 200 205 Tyr Pro Asn Trp Gly Asp Thr Val Glu Ile Asn Ser Trp Phe Ser Gln 210 215 220 Ser Gly Lys Ile Gly Met Gly Arg Asn Trp Leu Ile Ser Asp Cys Asn 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Ile Trp Ala Met Met Asn 245 250 255 Gln Asn Thr Arg Arg Phe Ser Lys Leu Pro Asn Glu Val Arg Gln Glu 260 265 270 Ile Ala Pro His Phe Val Asp Ala Pro Pro Val Ile Glu Asp Asn Asp 275 280 285 Arg Lys Leu His Lys Phe Asp Val Lys Thr Gly Asp Ser Ile Arg Lys 290 295 300 Gly Leu Thr Pro Gly Trp Asn Asp Leu Asp Val Asn Gln His Val Ser 305 310 315 320 Asn Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Ile Pro Thr Glu Val 325 330 335 Leu Glu Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu 340 345 350 Cys Gly Arg Glu Ser Val Leu Glu Ser Val Thr Ala Met Asn Pro Ser 355 360 365 Lys Val Gly Asp Arg Ser Gln Tyr Gln His Leu Leu Arg Leu Glu Asp 370 375 380 Gly Ala Asp Ile Met Lys Gly Arg Thr Glu Trp Arg Pro Lys Asn Ala 385 390 395 400 Gly Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser 405 410 415 Val Ser 99414PRTCuphea heterophylla 99Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Thr Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Arg Pro Leu Lys Pro Lys Phe Val Ala Asn Ala Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Ser Leu 50 55 60 Lys Ser Cys Ser Leu Lys Thr His Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Glu Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Lys Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Arg Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Glu Lys 355 360 365 Gly Phe Gly Pro Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Ala Ile Ala Phe Gly Glu Thr Ser Pro Gly Asp Ser 405 410 100414PRTCuphea heterophylla 100Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Thr Ser Ser Arg Pro Gly Lys Leu

Gly Asn Gly Ser Ser Ser Leu 20 25 30 Arg Pro Leu Lys Pro Lys Phe Val Ala Asn Ala Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Ser Leu 50 55 60 Lys Ser Cys Ser Leu Lys Thr His Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Lys Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Glu Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Lys Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Trp Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Lys 355 360 365 Gly Phe Gly Pro Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Thr Ile Ala Ser Gly Glu Thr Ser Pro Gly Asn Ser 405 410 101414PRTCuphea heterophylla 101Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Thr Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Arg Pro Leu Lys Pro Lys Phe Val Ala Asn Ala Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Ser Leu 50 55 60 Lys Ser Gly Ser Leu Lys Thr His Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Glu Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Lys Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Trp Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Lys 355 360 365 Gly Phe Gly Pro Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Thr Ile Ala Ser Gly Glu Thr Ser Pro Gly Asn Ser 405 410 102414PRTCuphea heterophylla 102Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Thr Ser Ser Arg Leu Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Arg Pro Leu Lys Pro Lys Phe Val Ala Asn Ala Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Ser Leu 50 55 60 Lys Ser Gly Ser Leu Lys Thr Gln Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Pro Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Glu Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Lys Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Trp Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Glu Lys 355 360 365 Gly Phe Gly Pro Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Thr Ile Ala Ser Gly Glu Thr Ser Pro Gly Asn Ser 405 410 103414PRTCuphea heterophylla 103Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Thr Ser Ser Arg Ala Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Arg Pro Leu Lys Pro Lys Phe Val Ala Asn Ala Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Ser Leu 50 55 60 Lys Ser Gly Ser Leu Lys Thr Gln Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Glu Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Lys Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Trp Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Lys 355 360 365 Gly Phe Gly Pro Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Thr Ile Ala Ser Gly Glu Thr Ser Pro Gly Asn Ser 405 410 104415PRTCuphea hyssopifolia 104Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Ala Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Ser Pro Leu Lys Pro Lys Leu Met Ala Asn Gly Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly Leu 50 55 60 Lys Ser Gly Ser Leu Lys Thr Gln Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Asp Gly Phe Gly Arg Thr Leu Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225 230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Arg Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Arg Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Lys 355 360 365 Gly Ser Gly Ser Gln Phe Gln His Leu Met Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Pro Ile Ala Ser Gly Glu Thr Ser Pro Gly Asp Ser Ser 405 410 415 105415PRTCuphea hyssopifolia 105Met Val Ala Thr Ala Ala Ser Ser Ala Phe Phe Pro Val Pro Ser Pro 1 5 10 15 Asp Ala Ser Ser Arg Pro Gly Lys Leu Gly Asn Gly Ser Ser Ser Leu 20 25 30 Ser Pro Leu Lys Pro Lys Leu Met Ala Asn Gly Gly Leu Gln Val Lys 35 40 45 Ala Asn Ala Ser Ala Pro Pro Lys Ile Asn Gly Ser Ser Val Gly Leu 50 55 60 Lys Ser Gly Ser Leu Lys Thr Gln Glu Asp Thr Pro Ser Ala Pro Pro 65 70 75 80 Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu Leu Ala 85 90 95 Ala Ile Thr Thr Val Phe Leu Ala Ala Glu Lys Gln Trp Met Met Leu 100 105 110 Asp Trp Lys Pro Lys Arg Pro Asp Met Leu Val Asp Pro Phe Gly Leu 115 120 125 Gly Arg Ile Val Gln Asp Gly Leu Val Phe Arg Gln Asn Phe Ser Ile 130 135 140 Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu Thr Val 145 150 155 160 Met Asn His Leu Gln Glu Thr Ala Leu Asn His Val Lys Ser Ala Gly 165 170 175 Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Tyr Lys Arg Asp 180 185 190 Leu Ile Trp Val Val Ala Lys Met Gln Val Met Val Asn Arg Tyr Pro 195 200 205 Thr Trp Gly Asp Thr Val Glu Val Asn Thr Trp Val Ala Lys Ser Gly 210 215 220 Lys Asn Gly Met Arg Arg Asp Trp Leu Ile Ser Asp Cys Asn Thr Gly 225

230 235 240 Glu Ile Leu Thr Arg Ala Ser Ser Val Trp Val Met Met Asn Gln Lys 245 250 255 Thr Arg Arg Leu Ser Lys Ile Pro Asp Glu Val Arg His Glu Ile Glu 260 265 270 Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Asp Asp Arg Lys 275 280 285 Leu Pro Lys Leu Asp Glu Lys Thr Ala Asp Ser Ile Arg Lys Gly Leu 290 295 300 Thr Pro Lys Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn Val 305 310 315 320 Lys Tyr Ile Gly Trp Ile Leu Glu Ser Thr Pro Pro Glu Val Leu Glu 325 330 335 Thr Gln Glu Leu Cys Ser Leu Thr Leu Glu Tyr Arg Arg Glu Cys Gly 340 345 350 Arg Glu Ser Val Leu Glu Ser Leu Thr Ala Val Asp Pro Ser Gly Lys 355 360 365 Gly Ser Gly Ser Gln Phe Gln His Leu Leu Arg Leu Glu Asp Gly Gly 370 375 380 Glu Ile Val Lys Gly Arg Thr Glu Trp Arg Pro Lys Thr Ala Gly Ile 385 390 395 400 Asn Gly Pro Ile Ala Ser Gly Glu Thr Ser Pro Gly Asp Ser Ser 405 410 415 106409PRTCuphea hyssopifolia 106Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Thr Ser Leu 20 25 30 Ser Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Ala Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Val Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys His 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Met Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Gln Glu Cys 340 345 350 Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Thr Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Lys Thr Ser Asn Gly Asn Ser Ile Ser 405 107417PRTCuphea hyssopifolia 107Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Ala Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Ser Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Ala Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Val Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys His 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Met Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Ala Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser Ile 405 410 415 Ser 108417PRTCuphea hyssopifolia 108Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Ala Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Ser Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Val Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Val Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys His 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Met Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Ala Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser Ile 405 410 415 Ser 109417PRTCuphea hyssopifolia 109Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Ala Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Val Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys His 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Met Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Ala Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser Ile 405 410 415 Ser 110409PRTCuphea hyssopifolia 110Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Thr Ser Leu 20 25 30 Ser Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Ala Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Ile Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Phe His 225 230 235 240 Thr Gly Asp Ile Leu Ile Arg Ala Thr Ser Val Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Gln Glu Cys 340 345 350 Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Thr Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Lys Thr Ser Asn Gly Asn Ser Ile Ser 405 111409PRTCuphea hyssopifolia 111Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Ser Ser Leu 20 25 30 Ser

Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Ala Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Ser Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Val Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Ile Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Phe His 225 230 235 240 Thr Gly Asp Ile Leu Ile Arg Ala Thr Ser Val Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Gln Glu Cys 340 345 350 Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Thr Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Lys Thr Ser Asn Gly Asn Ser Ile Ser 405 112417PRTCuphea hyssopifolia 112Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Thr Ser Leu 20 25 30 Ser Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Ala Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Val Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Cys His 225 230 235 240 Thr Gly Glu Ile Leu Ile Arg Ala Thr Ser Met Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Arg Glu Cys 340 345 350 Gly Gly Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Ala Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Thr Asn Gly Ala Ile Ser Thr Gly Lys Thr Ser Asn Gly Asn Ser Ile 405 410 415 Ser 113409PRTCuphea hyssopifolia 113Met Val Ala Ala Glu Ala Ser Ser Ala Leu Phe Ser Val Arg Thr Pro 1 5 10 15 Gly Thr Ser Pro Lys Pro Gly Lys Phe Gly Asn Trp Pro Thr Ser Leu 20 25 30 Ser Val Pro Phe Lys Ser Lys Ser Asn His Asn Gly Gly Phe Gln Val 35 40 45 Lys Ala Asn Ala Ser Ala Arg Pro Lys Ala Asn Gly Ser Ala Val Ser 50 55 60 Leu Lys Ser Gly Ser Leu Asp Thr Gln Glu Asp Thr Ser Ser Ser Ser 65 70 75 80 Ser Pro Pro Arg Thr Phe Ile Asn Gln Leu Pro Asp Trp Ser Met Leu 85 90 95 Leu Ser Ala Ile Thr Thr Val Phe Val Ala Ala Glu Lys Gln Trp Thr 100 105 110 Met Leu Asp Arg Lys Ser Lys Arg Pro Asp Met Leu Met Asp Pro Phe 115 120 125 Gly Val Asp Arg Val Val Gln Asp Gly Ala Val Phe Arg Gln Ser Phe 130 135 140 Ser Ile Arg Ser Tyr Glu Ile Gly Ala Asp Arg Thr Ala Ser Ile Glu 145 150 155 160 Thr Leu Met Asn Ile Phe Gln Glu Thr Ser Leu Asn His Cys Lys Ser 165 170 175 Ile Gly Leu Leu Asn Asp Gly Phe Gly Arg Thr Pro Glu Met Cys Lys 180 185 190 Arg Asp Leu Ile Trp Val Val Thr Lys Met His Ile Glu Val Asn Arg 195 200 205 Tyr Pro Thr Trp Gly Asp Thr Ile Glu Val Asn Thr Trp Val Ser Glu 210 215 220 Ser Gly Lys Thr Gly Met Gly Arg Asp Trp Leu Ile Ser Asp Phe His 225 230 235 240 Thr Gly Asp Ile Leu Ile Arg Ala Thr Ser Val Cys Ala Met Met Asn 245 250 255 Gln Lys Thr Arg Arg Phe Ser Lys Phe Pro Tyr Glu Val Arg Gln Glu 260 265 270 Leu Ala Pro His Phe Val Asp Ser Ala Pro Val Ile Glu Asp Tyr Gln 275 280 285 Lys Leu His Lys Leu Asp Val Lys Thr Gly Asp Ser Ile Cys Asn Gly 290 295 300 Leu Thr Pro Arg Trp Asn Asp Leu Asp Val Asn Gln His Val Asn Asn 305 310 315 320 Val Lys Tyr Ile Gly Trp Ile Leu Glu Ser Val Pro Thr Glu Val Phe 325 330 335 Glu Thr Gln Glu Leu Cys Gly Leu Thr Leu Glu Tyr Arg Gln Glu Cys 340 345 350 Gly Arg Asp Ser Val Leu Glu Ser Val Thr Ala Met Asp Pro Ser Lys 355 360 365 Glu Gly Asp Arg Ser Leu Tyr Gln His Leu Leu Arg Leu Glu Asp Gly 370 375 380 Thr Asp Ile Ala Lys Gly Arg Thr Lys Trp Arg Pro Lys Asn Ala Gly 385 390 395 400 Lys Thr Ser Asn Gly Asn Ser Ile Ser 405 1146541DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 114gctcttcgcc gccgccactc ctgctcgagc gcgcccgcgc gtgcgccgcc agcgccttgg 60ccttttcgcc gcgctcgtgc gcgtcgctga tgtccatcac caggtccatg aggtctgcct 120tgcgccggct gagccactgc ttcgtccggg cggccaagag gagcatgagg gaggactcct 180ggtccagggt cctgacgtgg tcgcggctct gggagcgggc cagcatcatc tggctctgcc 240gcaccgaggc cgcctccaac tggtcctcca gcagccgcag tcgccgccga ccctggcaga 300ggaagacagg tgaggggggt atgaattgta cagaacaacc acgagccttg tctaggcaga 360atccctacca gtcatggctt tacctggatg acggcctgcg aacagctgtc cagcgaccct 420cgctgccgcc gcttctcccg cacgcttctt tccagcaccg tgatggcgcg agccagcgcc 480gcacgctggc gctgcgcttc gccgatctga ggacagtcgg ggaactctga tcagtctaaa 540cccccttgcg cgttagtgtt gccatccttt gcagaccggt gagagccgac ttgttgtgcg 600ccacccccca caccacctcc tcccagacca attctgtcac ctttttggcg aaggcatcgg 660cctcggcctg cagagaggac agcagtgccc agccgctggg ggttggcgga tgcacgctca 720ggtacccttt cttgcgctat gacacttcca gcaaaaggta gggcgggctg cgagacggct 780tcccggcgct gcatgcaaca ccgatgatgc ttcgaccccc cgaagctcct tcggggctgc 840atgggcgctc cgatgccgct ccagggcgag cgctgtttaa atagccaggc ccccgattgc 900aaagacatta tagcgagcta ccaaagccat attcaaacac ctagatcact accacttcta 960cacaggccac tcgagcttgt gatcgcactc cgctaagggg gcgcctcttc ctcttcgttt 1020cagtcacaac ccgcaaacgg cgcgccatgc tgctgcaggc cttcctgttc ctgctggccg 1080gcttcgccgc caagatcagc gcctccatga cgaacgagac gtccgaccgc cccctggtgc 1140acttcacccc caacaagggc tggatgaacg accccaacgg cctgtggtac gacgagaagg 1200acgccaagtg gcacctgtac ttccagtaca acccgaacga caccgtctgg gggacgccct 1260tgttctgggg ccacgccacg tccgacgacc tgaccaactg ggaggaccag cccatcgcca 1320tcgccccgaa gcgcaacgac tccggcgcct tctccggctc catggtggtg gactacaaca 1380acacctccgg cttcttcaac gacaccatcg acccgcgcca gcgctgcgtg gccatctgga 1440cctacaacac cccggagtcc gaggagcagt acatctccta cagcctggac ggcggctaca 1500ccttcaccga gtaccagaag aaccccgtgc tggccgccaa ctccacccag ttccgcgacc 1560cgaaggtctt ctggtacgag ccctcccaga agtggatcat gaccgcggcc aagtcccagg 1620actacaagat cgagatctac tcctccgacg acctgaagtc ctggaagctg gagtccgcgt 1680tcgccaacga gggcttcctc ggctaccagt acgagtgccc cggcctgatc gaggtcccca 1740ccgagcagga ccccagcaag tcctactggg tgatgttcat ctccatcaac cccggcgccc 1800cggccggcgg ctccttcaac cagtacttcg tcggcagctt caacggcacc cacttcgagg 1860ccttcgacaa ccagtcccgc gtggtggact tcggcaagga ctactacgcc ctgcagacct 1920tcttcaacac cgacccgacc tacgggagcg ccctgggcat cgcgtgggcc tccaactggg 1980agtactccgc cttcgtgccc accaacccct ggcgctcctc catgtccctc gtgcgcaagt 2040tctccctcaa caccgagtac caggccaacc cggagacgga gctgatcaac ctgaaggccg 2100agccgatcct gaacatcagc aacgccggcc cctggagccg gttcgccacc aacaccacgt 2160tgacgaaggc caacagctac aacgtcgacc tgtccaacag caccggcacc ctggagttcg 2220agctggtgta cgccgtcaac accacccaga cgatctccaa gtccgtgttc gcggacctct 2280ccctctggtt caagggcctg gaggaccccg aggagtacct ccgcatgggc ttcgaggtgt 2340ccgcgtcctc cttcttcctg gaccgcggga acagcaaggt gaagttcgtg aaggagaacc 2400cctacttcac caaccgcatg agcgtgaaca accagccctt caagagcgag aacgacctgt 2460cctactacaa ggtgtacggc ttgctggacc agaacatcct ggagctgtac ttcaacgacg 2520gcgacgtcgt gtccaccaac acctacttca tgaccaccgg gaacgccctg ggctccgtga 2580acatgacgac gggggtggac aacctgttct acatcgacaa gttccaggtg cgcgaggtca 2640agtgacaatt ggcagcagca gctcggatag tatcgacaca ctctggacgc tggtcgtgtg 2700atggactgtt gccgccacac ttgctgcctt gacctgtgaa tatccctgcc gcttttatca 2760aacagcctca gtgtgtttga tcttgtgtgt acgcgctttt gcgagttgct agctgcttgt 2820gctatttgcg aataccaccc ccagcatccc cttccctcgt ttcatatcgc ttgcatccca 2880accgcaactt atctacgctg tcctgctatc cctcagcgct gctcctgctc ctgctcactg 2940cccctcgcac agccttggtt tgggctccgc ctgtattctc ctggtactgc aacctgtaaa 3000ccagcactgc aatgctgatg cacgggaagt agtgggatgg gaacacaaat ggaaagctgt 3060atagggataa gaattcggcc gacaggacgc gcgtcaaagg tgctggtcgt gtatgccctg 3120gccggcaggt cgttgctgct gctggttagt gattccgcaa ccctgatttt ggcgtcttat 3180tttggcgtgg caaacgctgg cgcccgcgag ccgggccggc ggcgatgcgg tgccccacgg 3240ctgccggaat ccaagggagg caagagcgcc cgggtcagtt gaagggcttt acgcgcaagg 3300tacagccgct cctgcaaggc tgcgtggtgg aattggacgt gcaggtcctg ctgaagttcc 3360tccaccgcct caccagcgga caaagcaccg gtgtatcagg tccgtgtcat ccactctaaa 3420gaactcgact acgacctact gatggcccta gattcttcat caaaaacgcc tgagacactt 3480gcccaggatt gaaactccct gaagggacca ccaggggccc tgagttgttc cttccccccg 3540tggcgagctg ccagccaggc tgtacctgtg atcgaggctg gcgggaaaat aggcttcgtg 3600tgctcaggtc atgggaggtg caggacagct catgaaacgc caacaatcgc acaattcatg 3660tcaagctaat cagctatttc ctcttcacga gctgtaattg tcccaaaatt ctggtctacc 3720gggggtgatc cttcgtgtac gggcccttcc ctcaacccta ggtatgcgcg catgcggtcg 3780ccgcgcaact cgcgcgaggg ccgagggttt gggacgggcc gtcccgaaat gcagttgcac 3840ccggatgcgt ggcacctttt ttgcgataat ttatgcaatg gactgctctg caaaattctg 3900gctctgtcgc caaccctagg atcagcggcg taggatttcg taatcattcg tcctgatggg 3960gagctaccga ctaccctaat atcagcccga ctgcctgacg ccagcgtcca cttttgtgca 4020cacattccat tcgtgcccaa gacatttcat tgtggtgcga agcgtcccca gttacgctca 4080cctgtttccc gacctcctta ctgttctgtc gacagagcgg gcccacaggc cggtcgcagc 4140cactagtatg gccaccacct ccctggcctc cgccttctgc tccatgaagg ccgtgatgct 4200ggcccgcgac ggccgcggcc tgaagccccg ctcctccgac ctgcagctgc gcgccggcaa 4260cgcccagacc tccctgaaga tgatcaacgg caccaagttc tcctacaccg agtccctgaa 4320gaagctgccc gactggtcca tgctgttcgc cgtgatcacc accatcttct ccgccgccga 4380gaagcagtgg accaacctgg agtggaagcc caagcccaac cccccccagc tgctggacga 4440ccacttcggc ccccacggcc tggtgttccg ccgcaccttc gccatccgct cctacgaggt 4500gggccccgac cgctccacct ccatcgtggc cgtgatgaac cacctgcagg aggccgccct 4560gaaccacgcc aagtccgtgg gcatcctggg cgacggcttc ggcaccaccc tggagatgtc 4620caagcgcgac ctgatctggg tggtgaagcg cacccacgtg gccgtggagc gctaccccgc 4680ctggggcgac accgtggagg tggagtgctg ggtgggcgcc tccggcaaca acggccgccg 4740ccacgacttc ctggtgcgcg actgcaagac cggcgagatc ctgacccgct gcacctccct 4800gtccgtgatg atgaacaccc gcacccgccg cctgtccaag atccccgagg aggtgcgcgg 4860cgagatcggc cccgccttca tcgacaacgt ggccgtgaag gacgaggaga tcaagaagcc 4920ccagaagctg aacgactcca ccgccgacta catccagggc ggcctgaccc cccgctggaa 4980cgacctggac atcaaccagc acgtgaacaa catcaagtac gtggactgga tcctggagac 5040cgtgcccgac tccatcttcg agtcccacca catctcctcc ttcaccatcg agtaccgccg 5100cgagtgcacc cgcgactccg tgctgcagtc cctgaccacc gtgtccggcg gctcctccga 5160ggccggcctg gtgtgcgagc acctgctgca gctggagggc ggctccgagg tgctgcgcgc 5220caagaccgag tggcgcccca agctgtcctt ccgcggcatc tccgtgatcc ccgccgagtc 5280ctccgtgatg gactacaagg accacgacgg cgactacaag gaccacgaca tcgactacaa 5340ggacgacgac gacaagtgac tcgaggcagc agcagctcgg atagtatcga cacactctgg 5400acgctggtcg tgtgatggac tgttgccgcc acacttgctg ccttgacctg tgaatatccc 5460tgccgctttt atcaaacagc ctcagtgtgt ttgatcttgt gtgtacgcgc ttttgcgagt 5520tgctagctgc ttgtgctatt tgcgaatacc acccccagca tccccttccc tcgtttcata 5580tcgcttgcat cccaaccgca acttatctac gctgtcctgc tatccctcag cgctgctcct 5640gctcctgctc actgcccctc gcacagcctt ggtttgggct ccgcctgtat tctcctggta 5700ctgcaacctg taaaccagca ctgcaatgct gatgcacggg aagtagtggg atgggaacac 5760aaatggaaag ctgtataggg ataacagggt aatgagctct tgttttccag aaggagttgc 5820tccttgagcc tttcattctc agcctcgata acctccaaag ccgctctaat tgtggagggg 5880gttcgaattt aaaagcttgg aatgttggtt cgtgcgtctg gaacaagccc agacttgttg 5940ctcactggga aaaggaccat cagctccaaa aaacttgccg ctcaaaccgc gtacctctgc 6000tttcgcgcaa tctgccctgt tgaaatcgcc accacattca tattgtgacg cttgagcagt 6060ctgtaattgc ctcagaatgt ggaatcatct gccccctgtg cgagcccatg ccaggcatgt 6120cgcgggcgag gacacccgcc actcgtacag cagaccatta tgctacctca caatagttca 6180taacagtgac catatttctc gaagctcccc aacgagcacc tccatgctct gagtggccac 6240cccccggccc tggtgcttgc ggagggcagg tcaaccggca tggggctacc gaaatccccg 6300accggatccc accacccccg cgatgggaag aatctctccc cgggatgtgg gcccaccacc 6360agcacaacct gctggcccag gcgagcgtca aaccatacca cacaaatatc cttggcatcg 6420gccctgaatt ccttctgccg ctctgctacc cggtgcttct gtccgaagca ggggttgcta 6480gggatcgctc cgagtccgca aacccttgtc gcgtggcggg gcttgttcga gcttgaagag 6540c 65411154PRTUnknownDescription of Unknown N-terminal hydrophobic domain peptide 115Leu Pro Asp Trp 1

Claims

1. An isolated nucleic acid having at least 80% sequence identity to any of SEQ ID NOS: 21, 27, 30, 69, 72, or 78 or any equivalent sequences by virtue of the degeneracy of the genetic code.

2. An isolated nucleic acid sequence encoding a protein having at least 80% sequence identity to any of SEQ ID NO: 22, 25, 28, 67, 70, or 77, or a fragment thereof having acyl-ACP thioesterase activity.

3. The isolated nucleic acid of claim 2, wherein, the protein has acyl-ACP thioesterase activity operable to alter the fatty acid profile of an oil produced by a recombinant cell comprising that sequence.

4. A method of producing a recombinant cell that produces an altered fatty acid profile, the method comprising transforming the cell with a nucleic acid according to claim 1.

5. A host cell produced by the method of claim 4.

6. The host cell of claim 5, wherein the host cell is selected from a plant cell, a microbial cell, and a microalgal cell.

7. A method for producing an oil or oil-derived product, the method comprising cultivating a host cell of claim 5, and extracting oil produced thereby, optionally wherein the cultivation is heterotrophic growth on sugar.

8. The method of claim 7, further comprising producing a fatty acid, fuel, chemical, or other oil-derived product from the oil.

9. An oil produced by the method of claim 7, optionally having a fatty acid profile comprising at least 20% C14 or C16 fatty acids.

10. An oil-derived product produced by the method of claim 8.

11. The oil of claim 10, wherein the oil is produced by a microalgae and optionally, lacks C24-alpha sterols.

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