DISULFIDE STABILIZED DVD-LG MOLECULES

Abstract

The present disclosure provides a binding protein comprising: a polypeptide heavy chain comprising: VH.sub.1--(X.sub.1)n-VH.sub.2--CH (X.sub.2)y wherein VH.sub.1 is a first variable domain, VH.sub.2 is a second variable domain, CH is a constant domain, X.sub.1 represents an amino acid or peptide, X.sub.2 represents an Fc region, n is 0 or 1 and y is independently 1 or 2, and a polypeptide light chain comprising: VL.sub.1-(X.sub.1)n-VL.sub.2-C wherein VL1 is a first variable domain, VL.sub.2 is a second variable domain, C is a constant domain, X.sub.1 represents an amino acid or peptide and n is 0 or 1, wherein the heavy chain and light chain are aligned such that VH.sub.1 and VL.sub.1 form a first binding domain, and VH.sub.2 and VL.sub.2 form a second binding domain and wherein: there is a disulfide bond between VH.sub.1 and VL.sub.1, and/or there is a disulfide bond between VH.sub.2 and VL.sub.2, and use thereof in treatment.

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