Patent application title: NOVEL METALLOPROTEASES
Inventors:
Lilia M. Babe (Emerald Hills, CA, US)
Roopa Ghirnikar (Sunnyvale, CA, US)
Frits Goedegebuur (Vlaardingen, NL)
Xiaogang Gu (Shanghai, CN)
Marc Kolkman (Oegsteest, NL)
Marc Kolkman (Oegsteest, NL)
Jian Yao (Sunnyvale, CA, US)
Jian Yao (Sunnyvale, CA, US)
IPC8 Class: AC12N952FI
USPC Class:
435264
Class name: Chemistry: molecular biology and microbiology process of utilizing an enzyme or micro-organism to destroy hazardous or toxic waste, liberate, separate, or purify a preexisting compound or composition therefore; cleaning objects or textiles cleaning using a micro-organism or enzyme
Publication date: 2016-05-05
Patent application number: 20160122738
Abstract:
Aspects of the present compositions and methods relate to novel
metalloproteases, polynucleotides encoding the novel metalloproteases,
and compositions and methods for use thereof.Claims:
1. A polypeptide comprising an amino acid sequence having at least 60%,
80%, or 95% sequence identity to an amino acid sequence selected from the
group consisting of SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 and 38.
2-4. (canceled)
5. The polypeptide of claim 1, wherein said polypeptide is derived from a member of the order Bacillales or is derived from a Planococcus species.
6. The polypeptide of claim 5, wherein said Bacillales member is a Paenibacillaceae family member or a Paenibacillus spp.
7-8. (canceled)
9. The polypeptide of claim 1, wherein said polypeptide has protease activity.
10. The polypeptide of claim 9, wherein said protease activity comprises casein hydrolysis, collagen hydrolysis, elastin hydrolysis, keratin hydrolysis, soy protein hydrolysis or corn meal protein hydrolysis.
11. The polypeptide of claim 1, wherein said polypeptide retains at least 50% of its maximal activity between pH 4.5 and 10 and/or between 30.degree. C. and 70.degree. C.
12. (canceled)
13. The polypeptide of claim 1, wherein said polypeptide has cleaning activity in a detergent composition.
14. The polypeptide of claim 13, wherein said detergent composition is selected from an ADW detergent composition, a laundry detergent composition, a liquid laundry detergent composition, and a powder laundry detergent composition.
15-18. (canceled)
19. The polypeptide of claim 1, wherein said polypeptide is a recombinant polypeptide.
20. A composition comprising the polypeptide of claim 1.
21. The composition of claim 20, wherein said composition is a cleaning composition or a detergent composition.
22. (canceled)
23. The composition of claim 21, wherein said detergent composition is selected from the group consisting of a laundry detergent, a fabric softening detergent, a dishwashing detergent, and a hard-surface cleaning detergent.
24. The composition of any of claim 20, wherein said composition further comprises a surfactant; at least one calcium ion and/or zinc ion; at least one stabilizer; from about 0.001 to about 0.1 weight % of said polypeptide; at least one bleaching agent; at least one adjunct ingredient; and/or one or more additional enzymes or enzyme derivatives selected from the group consisting of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1,4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, additional metallopotease enzymes and combinations thereof.
25-31. (canceled)
32. The composition of claim 20, wherein said cleaning composition contains phosphate or is phosphate-free.
33-34. (canceled)
35. The composition of claim 20, wherein said composition is a granular, powder, solid, bar, liquid, tablet, gel, or paste composition.
36-38. (canceled)
39. A method of cleaning, comprising contacting a surface or an item with a cleaning composition comprising the polypeptide of claim 1.
40-41. (canceled)
42. The method of claim 39, wherein said item is dishware or fabric.
43-48. (canceled)
49. A method for producing the polypeptide of claim 1 comprising: a. stably transforming a host cell with an expression vector comprising a polynucleotide encoding the polypeptide of claim 1; b. cultivating said transformed host cell under conditions suitable for said host cell to produce said protease; and c. recovering said protease.
50-56. (canceled)
57. A nucleic acid sequence comprising a nucleic acid sequence: (i) having at least 70% identity to a sequence selected from the group consisting of SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34 and 39, or (ii) being capable of hybridizing to a probe derived from the polynucleotide sequence selected from the group consisting of SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34 and 39, under conditions of intermediate to high stringency, or (iii) being complementary to the polynucleotide sequence selected from the group consisting of SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34 and 39.
58. A vector comprising the nucleic acid sequence of claim 57.
59. A host cell transformed with the vector of claim 58.
60-61. (canceled)
62. A textile processing, animal feed, leather processing, feather processing, or corn soy protein processing composition comprising the polypeptide of claim 1.
63-67. (canceled)
Description:
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims benefit of priority from International patent applications Serial No. PCT/CN2013/076419; Serial No. PCT/CN2013/076387; Serial No. PCT/CN2013/076401; Serial No. PCT/CN2013/076406; Serial No. PCT/CN2013/076414; Serial No. PCT/CN2013/076384; Serial No. PCT/CN2013/076398; and Serial No. PCT/CN2013/076415; all filed on 29 May 2013, the contents of which are incorporated herein by reference in their entirety.
FIELD OF THE INVENTION
[0002] The present disclosure relates to proteases and variants thereof. Compositions containing the proteases are suitable for use in cleaning, food and feed as well as in a variety of other industrial applications.
BACKGROUND
[0003] Metalloproteases (MPs) are among the hydrolases that mediate nucleophilic attack on peptide bonds using a water molecule coordinated in the active site. In their case, a divalent ion, such as zinc, activates the water molecule. This metal ion is held in place by amino acid ligands, usually 3 in number. The clan MA consists of zinc-dependent MPs in which two of the zinc ligands are the histidines in the motif: HisGluXXHis (SEQ ID NO: 41). This Glu is the catalytic residue. These are two domain proteases with the active site between the domains. In subclan MA(E), also known as Glu-zincins, the 3rd ligand is a Glu located C-terminal to the HDXXH (SEQ ID NO: 42) motif. Members of the families: M1, 3, 4, 13, 27 and 34 are all secreted proteases, almost exclusively from bacteria (Rawlings and Salvessen (2013) Handbook of Proteolytic Enzymes, Elsevier Press). They are generally active at elevated temperatures and this stability is attributed to calcium binding. Thermolysin-like proteases are found in the M4 family as defined by MEROPS (Rawlings et al., (2012) Nucleic Acids Res 40:D343-D350). Although proteases have long been known in the art of industrial enzymes, there remains a need for novel proteases that are suitable for particular conditions and uses.
SUMMARY
[0004] The present disclosure provides novel metalloprotease enzymes, nucleic acids encoding the same, and compositions and methods related to the production and use thereof.
[0005] In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 3. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, Alicyclobacillaceae, Lactobacillaceae, or a Bacillus, Alicyclobacillus, Geobacillus, Exiguobacterium, Lactobacillus, or Paenibacillus spp., such as Paenibacillus polymyxa. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the Pseudococcidae, or a Planococcus spp., such as Planococcus donghaensis. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 9.5. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 30° C. and 70° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition.
[0006] In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 8. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or Brevibacillaceae, or a Bacillus, Brevibacillus, or Paenibacillus spp., such as Paenibacillus sp. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from Brevibacillus sp. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 10. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 70° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition.
[0007] In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 13. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or Brevibacillaceae, or a Bacillus, Geobacillus, Brevibacillus, or Paenibacillus spp., such as Paenibacillus humicus. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from Bacillus polymyxa. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 9.5. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 70° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition.
[0008] In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 18. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or Brevibacillaceae, or a Bacillus, Geobacillus, Brevibacillus, or Paenibacillus spp., such as Paenibacillus ehimensis. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from Brevibacillus sp. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 10.5. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 45° C. and 75° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition.
[0009] In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 23. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, Alicyclobacillaceae, Lactobacillaceae, or a Bacillus, Geobacillus, Alicyclobacillus, Brevibacillus, Paenibacillus, or Lactobacillus spp., such as Paenibacillus barcinonensis. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the family Pseudococcidae, or a Planococcus spp., such as Planococcus donghaensis. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 10. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 65° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition.
[0010] In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 28. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or a Bacillus, Brevibacillus, Paenibacillus, or Lactobacillus spp., such as Paenibacillus polymyxa. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the family Pseudococcidae, or a Planococcus spp., such as Planococcus donghaensis. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5 and 9.5. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 30° C. and 65° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition.
[0011] In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 33. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, or a Bacillus, Geobacillus, Brevibacillus, or Paenibacillus spp., such as Paenibacillus hunanensis. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from Bacillus polymyxa. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 4.5 and 9.0. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 70° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition.
[0012] In some embodiments, the invention is a polypeptide comprising an amino acid sequence having at least 60%, at least 80%, or at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 38. In some embodiments, the invention is any of the above, wherein said polypeptide is derived from a member of the order Bacillales; family Bacillaceae, Paenibacillaceae, Lactobacillaceae, or a Bacillus, Brevibacillus, Lactobacillus, Paenibacillus, or Geobacillus spp., such as Paenibacillus amylolyticus. In various embodiments of the invention, any of the above polypeptides has protease activity, such as azo-casein hydrolysis. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between pH 5.5 and 10. In various embodiments of the invention, any of the above polypeptides retains at least 50% of its maximal activity between 35° C. and 65° C. In various embodiments of the invention, any of the above polypeptides has cleaning activity in a detergent composition, such as an ADW, laundry, liquid laundry, or powder laundry detergent composition.
[0013] In some embodiments, the invention is a composition comprising any of the above, such as a cleaning or detergent composition. In some embodiments, the composition further comprises a surfactant, at least one calcium ion and/or zinc ion, at least one stabilizer, at least one bleaching agent, and can contain phosphate, or be phosphate-free. In some embodiments, the composition further comprises one or more additional enzymes or enzyme derivatives selected from the group consisting of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1,4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, and combinations thereof. In some embodiments, the composition is formulated at a pH of from about 5.5 to about 8.5. In some embodiments, the invention is a method of cleaning using any of the above polypeptides or compositions. In some embodiments, the invention is a textile processing composition, animal feed composition, leather processing composition, or feather processing composition.
BRIEF DESCRIPTION OF THE DRAWINGS
[0014] FIG. 1.1 provides a plasmid map of pGX085 (aprE-PspPro3), described in Example 1.2.
[0015] FIG. 1.2 provides a dose response curve of PspPro3 in the azo-casein assay.
[0016] FIG. 1.3 provides the pH profile of PspPro3.
[0017] FIG. 1.4 provides the temperature profile of PspPro3.
[0018] FIG. 1.5A shows dose response for cleaning of PA-S-38 microswatches by PspPro3 protein in ADW detergent at pH 6 and 8.
[0019] FIG. 1.5B shows dose response for cleaning of PA-S-38 microswatches shows by PspPro3 protein in ADW detergent at pH 6 and 8 in the presence of bleach.
[0020] FIG. 1.6 shows cleaning performance of PspPro3 protein in liquid laundry detergent.
[0021] FIG. 1.7 (SEQ ID NOS: 3, 44, and 45, respectively) shows alignment of PspPro3 with other protein homologs.
[0022] FIG. 1.8 provides the phylogenetic tree for PspPro3 and its homologs.
[0023] FIG. 2.1 provides a plasmid map of pGX084 (aprE-PspPro2), described in Example 2.2.
[0024] FIG. 2.2 provides a dose response curve of PspPro2 in the azo-casein assay.
[0025] FIG. 2.3 provides the pH profile of purified PspPro2.
[0026] FIG. 2.4 provides the temperature profile of purified PspPro2.
[0027] FIG. 2.5A shows dose response for cleaning performance of PspPro2 at pH 6 in AT dish detergent with bleach.
[0028] FIG. 2.5B shows dose response for cleaning performance of purified PspPro2 at pH 8 in AT detergent with bleach.
[0029] FIG. 2.6A shows cleaning performance of PspPro2 protein in liquid laundry detergent.
[0030] FIG. 2.6B shows cleaning performance of PspPro2 protein in powder laundry detergent.
[0031] FIG. 2.7 (SEQ ID NOS: 8, 46, and 45, respectively) shows alignment of PspPro2 with other protein homologs.
[0032] FIG. 2.8 provides the phylogenetic tree for PspPro2 and its homologs.
[0033] FIG. 3.1 provides a plasmid map of pGX150 (aprE-PhuPro2), described in Example 3.2.
[0034] FIG. 3.2 provides a dose response curve of PhuPro2 in the azo-casein assay.
[0035] FIG. 3.3 provides the pH profile of purified PhuPro2.
[0036] FIG. 3.4 provides the temperature profile of purified PhuPro2.
[0037] FIG. 3.5A shows dose response for c leaning performance of PhuPro2 in AT dish detergent at pH 6.
[0038] FIG. 3.5B shows dose response for cleaning performance of PhuPro2 in AT dish detergent at pH 8.
[0039] FIG. 3.6 (SEQ ID NOS: 13, 47 and 45, respectively) shows alignment of PhuPro2 with other protein homologs.
[0040] FIG. 3.7 provides the phylogenetic tree for PhuPro2 and its homologs.
[0041] FIG. 4.1 provides a plasmid map of pGX148 (aprE-PehPro1), described in Example 4.2.
[0042] FIG. 4.2 provides a dose response curve of PehPro1 in the azo-casein assay.
[0043] FIG. 4.3 provides the pH profile of purified PehPro1.
[0044] FIG. 4.4 provides the temperature profile of purified PehPro1.
[0045] FIG. 4.5A shows dose response for cleaning performance of PehPro1 at pH 6 in AT dish detergent with bleach.
[0046] FIG. 4.5B shows dose response for cleaning performance of purified PehPro1 at pH 8 in AT detergent with bleach.
[0047] FIG. 4.6 (SEQ ID NOS: 18, 48, and 45, respectively) shows alignment of PehPro1 with other protein homologs.
[0048] FIG. 4.7 provides the phylogenetic tree for PehPro1 and its homologs.
[0049] FIG. 5.1 provides a plasmid map of pGX147 (aprE-PbaPro1), described in Example 5.2.
[0050] FIG. 5.2 provides a dose response curve of PbaPro1 in the azo-casein assay.
[0051] FIG. 5.3 provides the pH profile of purified PbaPro1.
[0052] FIG. 5.4 provides the temperature profile of purified PbaPro1.
[0053] FIG. 5.5A shows dose response for cleaning of PA-S-38 microswatches by PbaPro1protein in ADW detergent at pH 6.
[0054] FIG. 5.5B shows dose response for cleaning of PA-S-38 microswatches shows by PbaPro1protein in ADW detergent at pH 8.
[0055] FIG. 5.6 (SEQ ID NOS: 23, 49, and 45, respectively) shows the alignment of PbaPro1 with protease homologs.
[0056] FIG. 5.7 provides the phylogenetic tree for PbaPro1 and its homologs.
[0057] FIG. 6.1 provides a plasmid map of pGX138 (aprE-PpoPro1), described in Example 6.2.
[0058] FIG. 6.2 provides a dose response curve of PpoPro1 in the azo-casein assay.
[0059] FIG. 6.3 provides the pH profile of purified PpoPro1.
[0060] FIG. 6.4 provides the temperature profile of purified PpoPro1.
[0061] FIG. 6.5A shows dose response for cleaning of PA-S-38 microswatches by PpoPro1protein in ADW detergent at pH 6 in the presence of bleach.
[0062] FIG. 6.5B shows dose response for cleaning of PA-S-38 microswatches shows by PpoPro1protein in ADW detergent at pH 8 in the presence of bleach.
[0063] FIG. 6.6 (SEQ ID NOS: 28, 50, and 45, respectively) shows the alignment of PpoPro1 with protease homologs.
[0064] FIG. 6.7 provides the phylogenetic tree for PpoPro1 and its homologs.
[0065] FIG. 7.1 provides a plasmid map of pGX149 (aprE-PhuPro1), described in Example 7.2.
[0066] FIG. 7.2 provides a dose response curve of PhuPro1 in the azo-casein assay.
[0067] FIG. 7.3 provides the pH profile of purified PhuPro1.
[0068] FIG. 7.4 provides the temperature profile of purified PhuPro1.
[0069] FIG. 7.5A shows dose response for cleaning of PA-S-38 microswatches by PhuPro1 protein in ADW detergent at pH 6.
[0070] FIG. 7.5B shows dose response for cleaning of PA-S-38 microswatches shows by Phu Pro1protein in ADW detergent at pH 8.
[0071] FIG. 7.6 (SEQ ID NOS: 33, 51, and 45, respectively) shows alignment of PhuPro1 with other protein homologs.
[0072] FIG. 7.7 provides the phylogenetic tree for PhuPro1 and its homologs.
[0073] FIGS. 7.8A and 7.8B show cleaning performances of PhuPro1 and Purafect® Prime HA proteases.
[0074] FIG. 8.1 provides a plasmid map of pGX146 (aprE-PamPro1), described in Example 8.2.
[0075] FIG. 8.2 provides a dose response curve of PamPro1 in the azo-casein assay.
[0076] FIG. 8.3 provides the pH profile of purified PamPro1.
[0077] FIG. 8.4 provides the temperature profile of purified PamPro1.
[0078] FIG. 8.5A shows dose response for cleaning of PA-S-38 microswatches by PamPro1 protein in ADW detergent at pH 6.
[0079] FIG. 8.5B shows dose response for cleaning of PA-S-38 microswatches shows by PamPro1 protein in ADW detergent at pH 8.
[0080] FIG. 8.6 (SEQ ID NOS: 38, 52, and 45, respectively) shows the alignment of PamPro1 with protease homologs.
[0081] FIG. 8.7 provides the phylogenetic tree for PamPro1 and its homologs.
[0082] FIGS. 9.1A thru 9.1D (SEQ ID NOS: 53-62, 38, 23, 13, 63, 8, 28, 64, 3, 18, 33, 65-68, respectively) show the alignment of the various Paenibacillus metalloproteases with other bacterial metalloprotease homologs.
[0083] FIG. 9.2 provides the phylogenetic tree of the various Paenibacillus metalloproteases with other bacterial metalloprotease homologs.
DETAILED DESCRIPTION
[0084] The present invention provides novel metalloprotease enzymes, especially enzymes useful for detergent compositions cloned from various Paenibacillus sp. The compositions and methods are based, in part, on the observation that the novel metalloproteases of the present invention have proteolytic activity in the presence of detergent compositions. This feature makes metalloproteases of the present invention particularly well suited to and useful in a variety of cleaning applications where the enzyme can hydrolyze polypeptides in the presence of surfactants and other components found in detergent compositions. The invention includes compositions comprising at least one of the novel metalloprotease enzymes set forth herein. Some such compositions comprise detergent compositions. The metalloprotease enzymes of the present invention can be combined with other enzymes useful in detergent compositions. The invention also provides methods of cleaning using metalloprotease enzymes of the present invention.
DEFINITIONS AND ABBREVIATIONS
[0085] Unless otherwise indicated, the practice of the present invention involves conventional techniques commonly used in molecular biology, protein engineering, microbiology, and recombinant DNA technology, which are within the skill of the art. Such techniques are known to those of skill in the art and are described in numerous texts and reference works well known to those of skill in the art. All patents, patent applications, articles and publications mentioned herein, both supra and infra, are hereby expressly incorporated herein by reference.
[0086] Unless defined otherwise herein, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Many technical dictionaries are known to those of skill in the art. Although any methods and materials similar or equivalent to those described herein find use in the practice of the present invention, some suitable methods and materials are described herein. Accordingly, the terms defined immediately below are more fully described by reference to the Specification as a whole. Also, as used herein, the singular "a", "an" and "the" includes the plural reference unless the context clearly indicates otherwise. Unless otherwise indicated, nucleic acids are written left to right in 5' to 3' orientation; amino acid sequences are written left to right in amino to carboxy orientation, respectively. It is to be understood that this invention is not limited to the particular methodology, protocols, and reagents described, as these may vary, depending upon the context they are used by those of skill in the art.
[0087] Furthermore, the headings provided herein are not limitations of the various aspects or embodiments of the invention.
[0088] It is intended that every maximum numerical limitation given throughout this specification includes every lower numerical limitation, as if such lower numerical limitations were expressly written herein. Every minimum numerical limitation given throughout this specification will include every higher numerical limitation, as if such higher numerical limitations were expressly written herein. Every numerical range given throughout this specification will include every narrower numerical range that falls within such broader numerical range, as if such narrower numerical ranges were all expressly written herein.
[0089] As used herein, the terms "protease" and "proteinase" refer to an enzyme that has the ability to break down proteins and peptides. A protease has the ability to conduct "proteolysis," by hydrolysis of peptide bonds that link amino acids together in a peptide or polypeptide chain forming the protein. This activity of a protease as a protein-digesting enzyme is referred to as "proteolytic activity." Many well known procedures exist for measuring proteolytic activity (See e.g., Kalisz, "Microbial Proteinases," In: Fiechter (ed.), Advances in Biochemical Engineering/Biotechnology, (1988)). For example, proteolytic activity may be ascertained by comparative assays which analyze the respective protease's ability to hydrolyze a suitable substrate. Exemplary substrates useful in the analysis of protease or proteolytic activity, include, but are not limited to, di-methyl casein (Sigma C-9801), bovine collagen (Sigma C-9879), bovine elastin (Sigma E-1625), and bovine keratin (ICN Biomedical 902111). Colorimetric assays utilizing these substrates are well known in the art (See e.g., WO 99/34011 and U.S. Pat. No. 6,376,450, both of which are incorporated herein by reference). The pNA peptidyl assay (See e.g., Del Mar et al., Anal. Biochem. 99:316-320
[1979]) also finds use in determining the active enzyme concentration. This assay measures the rate at which p-nitroaniline is released as the enzyme hydrolyzes a soluble synthetic substrate, such as succinyl-alanine-alanine-proline-phenylalanine-p-nitroanilide (suc-AAPF-pNA) (SEQ ID NO: 43). The rate of production of yellow color from the hydrolysis reaction is measured at 410 nm on a spectrophotometer and is proportional to the active enzyme concentration. In addition, absorbance measurements at 280 nanometers (nm) can be used to determine the total protein concentration in a sample of purified protein. The activity on substrate/protein concentration gives the enzyme specific activity.
[0090] As used herein, the term "variant polypeptide" refers to a polypeptide comprising an amino acid sequence that differs in at least one amino acid residue from the amino acid sequence of a parent or reference polypeptide (including but not limited to wild-type polypeptides).
[0091] As used herein, "the genus Bacillus" includes all species within the genus "Bacillus," as known to those of skill in the art, including but not limited to B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. clausii, B. halodurans, B. megaterium, B. coagulans, B. circulans, and B. thuringiensis. It is recognized that the genus Bacillus continues to undergo taxonomical reorganization. Thus, it is intended that the genus include species that have been reclassified, including but not limited to such organisms as B. stearothermophilus, which is now named "Geobacillus stearothermophilus." The production of resistant endospores under stressful environmental conditions is considered the defining feature of the genus Bacillus, although this characteristic also applies to the recently named Alicyclobacillus, Amphibacillus, Aneurinibacillus, Paenibacillus, Brevibacillus, Filobacillus, Gracilibacillus, Halobacillus, Paenibacillus, Salibacillus, Thermobacillus, Ureibacillus, and Virgibacillus.
[0092] The terms "polynucleotide" and "nucleic acid," which are used interchangeably herein, refer to a polymer of any length of nucleotide monomers covalently bonded in a chain. DNA (deoxyribonucleic acid), a polynucleotide comprising deoxyribonucleotides, and RNA (ribonucleic acid), a polymer of ribonucleotides, are examples of polynucleotides or nucleic acids having distinct biological function. Polynucleotides or nucleic acids include, but are not limited to, a single-, double- or triple-stranded DNA, genomic DNA, cDNA, RNA, DNA-RNA hybrid, or a polymer comprising purine and pyrimidine bases, or other natural, chemically, biochemically modified, non-natural or derivatized nucleotide bases. The following are non-limiting examples of polynucleotides: genes, gene fragments, chromosomal fragments, expressed sequence tag(s) (EST(s)), exons, introns, messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), ribozymes, complementary DNA (cDNA), recombinant polynucleotides, branched polynucleotides, plasmids, vectors, isolated DNA of any sequence, isolated RNA of any sequence, nucleic acid probes, and primers.
[0093] As used herein, the term "mutation" refers to changes made to a reference amino acid or nucleic acid sequence. It is intended that the term encompass substitutions, insertions and deletions.
[0094] As used herein, the term "vector" refers to a nucleic acid construct used to introduce or transfer nucleic acid(s) into a target cell or tissue. A vector is typically used to introduce foreign DNA into a cell or tissue. Vectors include plasmids, cloning vectors, bacteriophages, viruses (e.g., viral vector), cosmids, expression vectors, shuttle vectors, and the like. A vector typically includes an origin of replication, a multicloning site, and a selectable marker. The process of inserting a vector into a target cell is typically referred to as transformation. The present invention includes, in some embodiments, a vector that comprises a DNA sequence encoding a metalloprotease polypeptide (e.g., precursor or mature metalloprotease polypeptide) that is operably linked to a suitable prosequence (e.g., secretory, signal peptide sequence, etc.) capable of effecting the expression of the DNA sequence in a suitable host, and the folding and translocation of the recombinant polypeptide chain.
[0095] As used herein, the term "expression cassette," "expression plasmid" or "expression vector" refers to a nucleic acid construct or vector generated recombinantly or synthetically for the expression of a nucleic acid of interest in a target cell. An expression vector or expression cassette typically comprises a promoter nucleotide sequence that drives expression of the foreign nucleic acid. The expression vector or cassette also typically includes any other specified nucleic acid elements that permit transcription of a particular nucleic acid in a target cell. A recombinant expression cassette can be incorporated into a plasmid, chromosome, mitochondrial DNA, plastid DNA, virus, or nucleic acid fragment. Many prokaryotic and eukaryotic expression vectors are commercially available.
[0096] In some embodiments, the ends of the sequence are closed such that the DNA construct forms a closed circle. The nucleic acid sequence of interest, which is incorporated into the DNA construct, using techniques well known in the art, may be a wild-type, mutant, or modified nucleic acid. In some embodiments, the DNA construct comprises one or more nucleic acid sequences homologous to the host cell chromosome. In other embodiments, the DNA construct comprises one or more non-homologous nucleotide sequences. Once the DNA construct is assembled in vitro, it may be used, for example, to: 1) insert heterologous sequences into a desired target sequence of a host cell; and/or 2) mutagenize a region of the host cell chromosome (i.e., replace an endogenous sequence with a heterologous sequence); 3) delete target genes; and/or 4) introduce a replicating plasmid into the host. "DNA construct" is used interchangeably herein with "expression cassette."
[0097] As used herein, a "plasmid" refers to an extrachromosomal DNA molecule which is capable of replicating independently from the chromosomal DNA. A plasmid is double stranded (ds) and may be circular and is typically used as a cloning vector.
[0098] As used herein in the context of introducing a nucleic acid sequence into a cell, the term "introduced" refers to any method suitable for transferring the nucleic acid sequence into the cell. Such methods for introduction include but are not limited to protoplast fusion, transfection, transformation, electroporation, conjugation, and transduction (See e.g., Ferrari et al., "Genetics," in Hardwood et al. (eds.), Bacillus, Plenum Publishing Corp., pp. 57-72
[1989]).
[0099] Transformation refers to the genetic alteration of a cell which results from the uptake, optional genomic incorporation, and expression of genetic material (e.g., DNA).
[0100] As used herein, a nucleic acid is "operably linked" with another nucleic acid sequence when it is placed into a functional relationship with another nucleic acid sequence. For example, a promoter or enhancer is operably linked to a nucleotide coding sequence if the promoter affects the transcription of the coding sequence. A ribosome binding site may be operably linked to a coding sequence if it is positioned so as to facilitate translation of the coding sequence. Typically, "operably linked" DNA sequences are contiguous. However, enhancers do not have to be contiguous. Linking is accomplished by ligation at convenient restriction sites. If such sites do not exist, synthetic oligonucleotide adaptors or linkers may be used in accordance with conventional practice.
[0101] As used herein the term "gene" refers to a polynucleotide (e.g., a DNA segment), that encodes a polypeptide and includes regions preceding and following the coding regions as well as intervening sequences (introns) between individual coding segments (exons).
[0102] As used herein, "recombinant" when used with reference to a cell typically indicates that the cell has been modified by the introduction of a foreign nucleic acid sequence or that the cell is derived from a cell so modified. For example, a recombinant cell may comprise a gene not found in identical form within the native (non-recombinant) form of the cell, or a recombinant cell may comprise a native gene (found in the native form of the cell) but which has been modified and re-introduced into the cell. A recombinant cell may comprise a nucleic acid endogenous to the cell that has been modified without removing the nucleic acid from the cell; such modifications include those obtained by gene replacement, site-specific mutation, and related techniques known to those of ordinary skill in the art. Recombinant DNA technology includes techniques for the production of recombinant DNA in vitro and transfer of the recombinant DNA into cells where it may be expressed or propagated, thereby producing a recombinant polypeptide. "Recombination," "recombining," and "recombined" of polynucleotides or nucleic acids refer generally to the assembly or combining of two or more nucleic acid or polynucleotide strands or fragments to generate a new polynucleotide or nucleic acid. The recombinant polynucleotide or nucleic acid is sometimes referred to as a chimera. A nucleic acid or polypeptide is "recombinant" when it is artificial or engineered.
[0103] A nucleic acid or polynucleotide is said to "encode" a polypeptide if, in its native state or when manipulated by methods known to those of skill in the art, it can be transcribed and/or translated to produce the polypeptide or a fragment thereof. The anti-sense strand of such a nucleic acid is also said to encode the sequence.
[0104] "Host strain" or "host cell" refers to a suitable host for an expression vector comprising a DNA sequence of interest.
[0105] A "protein" or "polypeptide" comprises a polymeric sequence of amino acid residues. The terms "protein" and "polypeptide" are used interchangeably herein. The single and 3-letter code for amino acids as defined in conformity with the IUPAC-IUB Joint Commission on Biochemical Nomenclature (JCBN) is used through out this disclosure. The single letter X refers to any of the twenty amino acids. It is also understood that a polypeptide may be coded for by more than one nucleotide sequence due to the degeneracy of the genetic code. Mutations can be named by the one letter code for the parent amino acid, followed by a position number and then the one letter code for the variant amino acid. For example, mutating glycine (G) at position 87 to serine (S) is represented as "G087S" or "G87S". Mutations can also be named by using the three letter code for an amino acid followed by its position in the polypeptide chain as counted from the N-terminus; for example, Ala10 for alanine at position 10. Multiple mutations are indicated by inserting a "-" between the mutations. Mutations at positions 87 and 90 are represented as either "G087S-A090Y" or "G87S-A90Y" or "G87S+A90Y" or "G087S+A090Y". For deletions, the one letter code "Z" is used. For an insertion relative to the parent sequence, the one letter code "Z" is on the left side of the position number. For a deletion, the one letter code "Z" is on the right side of the position number. For insertions, the position number is the position number before the inserted amino acid(s), plus 0.01 for each amino acid. For example, an insertion of three amino acids alanine (A), serine (S) and tyrosine (Y) between position 87 and 88 is shown as "Z087.01A-Z087.02S-Z087.03Y." Thus, combining all the mutations above plus a deletion at position 100 is: "G087S-Z087.01A-Z087.02S-Z087.03Y-A090Y-A100Z." When describing modifications, a position followed by amino acids listed in parentheses indicates a list of substitutions at that position by any of the listed amino acids. For example, 6(L,I) means position 6 can be substituted with a leucine or isoleucine.
[0106] A "prosequence" or "propetide sequence" refers to an amino acid sequence between the signal peptide sequence and mature protease sequence that is necessary for the proper folding and secretion of the protease; they are sometimes referred to as intramolecular chaperones. Cleavage of the prosequence or propeptide sequence results in a mature active protease. Bacterial metalloproteases are often expressed as pro-enzymes.
[0107] The term "signal sequence" or "signal peptide" refers to a sequence of amino acid residues that may participate in the secretion or direct transport of the mature or precursor form of a protein. The signal sequence is typically located N-terminal to the precursor or mature protein sequence. The signal sequence may be endogenous or exogenous. A signal sequence is normally absent from the mature protein. A signal sequence is typically cleaved from the protein by a signal peptidase after the protein is transported.
[0108] The term "mature" form of a protein, polypeptide, or peptide refers to the functional form of the protein, polypeptide, or peptide without the signal peptide sequence and propeptide sequence.
[0109] The term "precursor" form of a protein or peptide refers to a mature form of the protein having a prosequence operably linked to the amino or carbonyl terminus of the protein. The precursor may also have a "signal" sequence operably linked to the amino terminus of the prosequence. The precursor may also have additional polypeptides that are involved in post-translational activity (e.g., polypeptides cleaved therefrom to leave the mature form of a protein or peptide).
[0110] The term "wild-type" in reference to an amino acid sequence or nucleic acid sequence indicates that the amino acid sequence or nucleic acid sequence is native or naturally occurring sequence. As used herein, the term "naturally-occurring" refers to anything (e.g., proteins, amino acids, or nucleic acid sequences) that are found in nature.
[0111] As used herein, the term "non-naturally occurring" refers to anything that is not found in nature (e.g., recombinant nucleic acids and protein sequences produced in the laboratory), as modification of the wild-type sequence.
[0112] As used herein with regard to amino acid residue positions, "corresponding to" or "corresponds to" or "corresponds" refers to an amino acid residue at the enumerated position in a protein or peptide, or an amino acid residue that is analogous, homologous, or equivalent to an enumerated residue in a protein or peptide. As used herein, "corresponding region" generally refers to an analogous position in a related proteins or a reference protein.
[0113] The terms "derived from" and "obtained from" refer to not only a protein produced or producible by a strain of the organism in question, but also a protein encoded by a DNA sequence isolated from such strain and produced in a host organism containing such DNA sequence. Additionally, the term refers to a protein which is encoded by a DNA sequence of synthetic and/or cDNA origin and which has the identifying characteristics of the protein in question. To exemplify, "proteases derived from Bacillus" refers to those enzymes having proteolytic activity which are naturally produced by Bacillus, as well as to serine proteases like those produced by Bacillus sources but which through the use of genetic engineering techniques are produced by non-Bacillus organisms transformed with a nucleic acid encoding the serine proteases.
[0114] The term "identical" in the context of two nucleic acids or polypeptidesequences refers to the residues in the two sequences that are the same when aligned for maximum correspondence, as measured using one of the following sequence comparison or analysis algorithms.
[0115] As used herein, "homologous genes" refers to a pair of genes from different, but usually related species, which correspond to each other and which are identical or very similar to each other. The term encompasses genes that are separated by speciation (i.e., the development of new species) (e.g., orthologous genes), as well as genes that have been separated by genetic duplication (e.g., paralogous genes).
[0116] As used herein, "% identity or percent identity" refers to sequence similarity. Percent identity may be determined using standard techniques known in the art (See e.g., Smith and Waterman, Adv. Appl. Math. 2:482
[1981]; Needleman and Wunsch, J. Mol. Biol. 48:443
[1970]; Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444
[1988]; software programs such as GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package (Genetics Computer Group, Madison, Wis.); and Devereux et al., Nucl. Acid Res. 12:387-395
[1984]). One example of a useful algorithm is PILEUP. PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pair-wise alignments. It can also plot a tree showing the clustering relationships used to create the alignment. PILEUP uses a simplification of the progressive alignment method of Feng and Doolittle (See, Feng and Doolittle, J. Mol. Evol. 35:351-360
[1987]). The method is similar to that described by Higgins and Sharp (See, Higgins and Sharp, CABIOS 5:151-153
[1989]). Useful PILEUP parameters include a default gap weight of 3.00, a default gap length weight of 0.10, and weighted end gaps. Other useful algorithm is the BLAST algorithms described by Altschul et al., (See, Altschul et al., J. Mol. Biol. 215:403-410
[1990]; and Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873-5787
[1993]). The BLAST program uses several search parameters, most of which are set to the default values.
[0117] The NCBI BLAST algorithm finds the most relevant sequences in terms of biological similarity but is not recommended for query sequences of less than 20 residues (Altschul, S F et al. (1997) Nucleic Acids Res. 25:3389-3402 and Schaffer, A A et al. (2001) Nucleic Acids Res. 29:2994-3005). Example default BLAST parameters for a nucleic acid sequence searches are:
[0118] Neighboring words threshold: 11
[0119] E-value cutoff: 10
[0120] Scoring Matrix: NUC.3.1 (match=1, mismatch=-3)
[0121] Gap Opening: 5
[0122] Gap Extension: 2 and the following parameters for amino acid sequence searches:
[0123] Word size: 3
[0124] E-value cutoff: 10
[0125] Scoring Matrix: BLOSUM62
[0126] Gap Opening: 11
[0127] Gap extension: 1
[0128] A percent (%) amino acid sequence identity value is determined by the number of matching identical residues divided by the total number of residues of the "reference" sequence including any gaps created by the program for optimal/maximum alignment. If a sequence is 90% identical to SEQ ID NO: A, SEQ ID NO: A is is the "reference" sequence. BLAST algorithms refer the "reference" sequence as "query" sequence.
[0129] The CLUSTAL W algorithm is another example of a sequence alignment algorithm. See Thompson et al. (1994) Nucleic Acids Res. 22:4673-4680. Default parameters for the CLUSTAL W algorithm are:
[0130] Gap opening penalty: 10.0
[0131] Gap extension penalty: 0.05
[0132] Protein weight matrix: BLOSUM series
[0133] DNA weight matrix: IUB
[0134] Delay divergent sequences %: 40
[0135] Gap separation distance: 8
[0136] DNA transitions weight: 0.50
[0137] List hydrophilic residues: GPSNDQEKR
[0138] Use negative matrix: OFF
[0139] Toggle Residue specific penalties: ON
[0140] Toggle hydrophilic penalties: ON
[0141] Toggle end gap separation penalty OFF.
[0142] In CLUSTAL algorithms, deletions occurring at either terminus are included. For example, a variant with five amino acid deletion at either terminus (or within the polypeptide) of a polypeptide of 500 amino acids would have a percent sequence identity of 99% (495/500 identical residues×100) relative to the "reference" polypeptide. Such a variant would be encompassed by a variant having "at least 99% sequence identity" to the polypeptide.
[0143] A polypeptide of interest may be said to be "substantially identical" to a reference polypeptide if the polypeptide of interest comprises an amino acid sequence having at least about 60%, least about 65%, least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 99.5% sequence identity to the amino acid sequence of the reference polypeptide. The percent identity between two such polypeptides can be determined manually by inspection of the two optimally aligned polypeptide sequences or by using software programs or algorithms (e.g., BLAST, ALIGN, CLUSTAL) using standard parameters. One indication that two polypeptides are substantially identical is that the first polypeptide is immunologically cross-reactive with the second polypeptide. Typically, polypeptides that differ by conservative amino acid substitutions are immunologically cross-reactive. Thus, a polypeptide is substantially identical to a second polypeptide, for example, where the two peptides differ only by a conservative amino acid substitution or one or more conservative amino acid substitutions.
[0144] A nucleic acid of interest may be said to be "substantially identical" to a reference nucleic acid if the nucleic acid of interest comprises a nucleotide sequence having least about 60%, least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 99.5% sequence identity to the nucleotide sequence of the reference nucleic acid. The percent identity between two such nucleic acids can be determined manually by inspection of the two optimally aligned nucleic acid sequences or by using software programs or algorithms (e.g., BLAST, ALIGN, CLUSTAL) using standard parameters. One indication that two nucleic acid sequences are substantially identical is that the two nucleic acid molecules hybridize to each other under stringent conditions (e.g., within a range of medium to high stringency).
[0145] A nucleic acid or polynucleotide is "isolated" when it is at least partially or completely separated from other components, including but not limited to for example, other proteins, nucleic acids, cells, etc. Similarly, a polypeptide, protein or peptide is "isolated" when it is at least partially or completely separated from other components, including but not limited to for example, other proteins, nucleic acids, cells, etc. On a molar basis, an isolated species is more abundant than are other species in a composition. For example, an isolated species may comprise at least about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, or about 100% (on a molar basis) of all macromolecular species present. Preferably, the species of interest is purified to essential homogeneity (i.e., contaminant species cannot be detected in the composition by conventional detection methods). Purity and homogeneity can be determined using a number of techniques well known in the art, such as agarose or polyacrylamide gel electrophoresis of a nucleic acid or a protein sample, respectively, followed by visualization upon staining. If desired, a high-resolution technique, such as high performance liquid chromatography (HPLC) or a similar means can be utilized for purification of the material.
[0146] "Hybridization" refers to the process by which one strand of nucleic acid forms a duplex with, i.e., base pairs with, a complementary strand. A nucleic acid sequence is considered to be "selectively hybridizable" to a reference nucleic acid sequence if the two sequences specifically hybridize to one another under moderate to high stringency hybridization and wash conditions. Hybridization conditions are based on the melting temperature (Tm) of the nucleic acid binding complex or probe. For example, "maximum stringency" typically occurs at about Tm-5° C. (5° below the Tm of the probe); "high stringency" at about 5-10° C. below the Tm; "intermediate stringency" at about 10-20° C. below the Tm of the probe; and "low stringency" at about 20-25° C. below the Tm. Functionally, maximum stringency conditions can be used to identify sequences having strict identity or near-strict identity with the hybridization probe; while intermediate or low stringency hybridization can be used to identify or detect polynucleotide sequence homologs.
[0147] Moderate and high stringency hybridization conditions are well known in the art. Stringent hybridization conditions are exemplified by hybridization under the following conditions: 65° C. and 0.1×SSC (where 1×SSC=0.15 M NaCl, 0.015 M Na3 citrate, pH 7.0). Hybridized, duplex nucleic acids are characterized by a melting temperature (Tm), where one half of the hybridized nucleic acids are unpaired with the complementary strand. Mismatched nucleic acids within the duplex lower the Tm. Very stringent hybridization conditions involve 68° C. and 0.1×SSC. A nucleic acid encoding a variant metalloprotease can have a Tm reduced by 1° C.-3° C. or more compared to a duplex formed between the nucleic acid and its identical complement.
[0148] Another example of high stringency conditions includes hybridization at about 42° C. in 50% formamide, 5×SSC, 5×Denhardt's solution, 0.5% SDS and 100 μg/ml denatured carrier DNA followed by washing two times in 2×SSC and 0.5% SDS at room temperature and two additional times in 0.1×SSC and 0.5% SDS at 42° C. An example of moderate stringent conditions include an overnight incubation at 37° C. in a solution comprising 20% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextran sulfate and 20 mg/ml denatured sheared salmon sperm DNA, followed by washing the filters in 1×SSC at about 37-50° C. Those of skill in the art know how to adjust the temperature, ionic strength, etc. to accommodate factors such as probe length and the like.
[0149] The term "purified" as applied to nucleic acids or polypeptides generally denotes a nucleic acid or polypeptide that is essentially free from other components as determined by analytical techniques well known in the art (e.g., a purified polypeptide or polynucleotide forms a discrete band in an electrophoretic gel, chromatographic eluate, and/or a media subjected to density gradient centrifugation). For example, a nucleic acid or polypeptide that gives rise to essentially one band in an electrophoretic gel is "purified." A purified nucleic acid or polypeptide is at least about 50% pure, usually at least about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, about 99.5%, about 99.6%, about 99.7%, about 99.8% or more pure (e.g., percent by weight on a molar basis). In a related sense, the invention provides methods of enriching compositions for one or more molecules of the invention, such as one or more polypeptides or polynucleotides of the invention. A composition is enriched for a molecule when there is a substantial increase in the concentration of the molecule after application of a purification or enrichment technique. A substantially pure polypeptide or polynucleotide of the invention (e.g., substantially pure metalloprotease polypeptide or polynucleotide encoding a metalloprotease polypeptide of the invention, respectively) will typically comprise at least about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98, about 99%, about 99.5% or more by weight (on a molar basis) of all macromolecular species in a particular composition.
[0150] The term "enriched" refers to a compound, polypeptide, cell, nucleic acid, amino acid, or other specified material or component that is present in a composition at a relative or absolute concentration that is higher than a starting composition.
[0151] In a related sense, the invention provides methods of enriching compositions for one or more molecules of the invention, such as one or more polypeptides of the invention (e.g., one or more metalloprotease polypeptides of the invention) or one or more nucleic acids of the invention (e.g., one or more nucleic acids encoding one or more metalloprotease polypeptides of the invention). A composition is enriched for a molecule when there is a substantial increase in the concentration of the molecule after application of a purification or enrichment technique. A substantially pure polypeptide or polynucleotide will typically comprise at least about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98, about 99%, about 99.5% or more by weight (on a molar basis) of all macromolecular species in a particular composition.
[0152] As used herein, the term "functional assay" refers to an assay that provides an indication of a protein's activity. In some embodiments, the term refers to assay systems in which a protein is analyzed for its ability to function in its usual capacity. For example, in the case of a protease, a functional assay involves determining the effectiveness of the protease to hydrolyze a proteinaceous substrate.
[0153] The terms "modified nucleic acid sequence" and "modified gene" are used interchangeably herein to refer to a nucleic acid sequence that includes a deletion, insertion or interruption of naturally occurring (i.e., wild-type) nucleic acid sequence. In some embodiments, the expression product of the modified nucleic acid sequence is a truncated protein (e.g., if the modification is a deletion or interruption of the sequence). In some embodiments, the truncated protein retains biological activity. In alternative embodiments, the expression product of the modified nucleic acid sequence is an elongated protein (e.g., modifications comprising an insertion into the nucleic acid sequence). In some embodiments, a nucleotide insertion in the nucleic acid sequence leads to a truncated protein (e.g., when the insertion results in the formation of a stop codon). Thus, an insertion may result in either a truncated protein or an elongated protein as an expression product.
[0154] A "mutant" nucleic acid sequence typically refers to a nucleic acid sequence that has an alteration in at least one codon occurring in a host cell's wild-type sequence such that the expression product of the mutant nucleic acid sequence is a protein with an altered amino acid sequence relative to the wild-type protein. The expression product may have an altered functional capacity (e.g., enhanced enzymatic activity).
[0155] As used herein, the phrase "alteration in substrate specificity" refers to changes in the substrate specificity of an enzyme. In some embodiments, a change in substrate specificity is defined as a change in kcat and/or Km for a particular substrate, resulting from mutations of the enzyme or alteration of reaction conditions. The substrate specificity of an enzyme is determined by comparing the catalytic efficiencies it exhibits with different substrates. These determinations find particular use in assessing the efficiency of mutant enzymes, as it is generally desired to produce variant enzymes that exhibit greater ratios of kcat/Km for substrates of interest. However, it is not intended that the present invention be limited to any particular substrate composition or substrate specificity.
[0156] As used herein, "surface property" is used in reference to electrostatic charge, as well as properties such as the hydrophobicity and hydrophilicity exhibited by the surface of a protein. As used herein, the term "net charge" is defined as the sum of all charges present in a molecule. "Net charge changes" are made to a parent protein molecule to provide a variant that has a net charge that differs from that of the parent molecule (i.e., the variant has a net charge that is not the same as that of the parent molecule). For example, substitution of a neutral amino acid with a negatively charged amino acid or a positively charged amino acid with a neutral amino acid results in net charge of -1 with respect to the parent molecule. Substitution of a positively charged amino acid with a negatively charged amino acid results in a net charge of -2 with respect to the parent. Substitution of a neutral amino acid with a positively charged amino acid or a negatively charged amino acid with a neutral amino acid results in net charge of +1 with respect to the parent. Substitution of a negatively charged amino acid with a positively charged amino acid results in a net charge of +2 with respect to the parent. The net charge of a parent protein can also be altered by deletion and/or insertion of charged amino acids. A net change change applies to changes in charge of a variant versus a parent when measured at the same pH conditions.
[0157] The terms "thermally stable" and "thermostable" and "thermostability" refer to proteases that retain a specified amount of enzymatic activity after exposure to identified temperatures over a given period of time under conditions prevailing during the proteolytic, hydrolyzing, cleaning or other process of the invention, while being exposed to altered temperatures. "Altered temperatures" encompass increased or decreased temperatures. In some embodiments, the proteases retain at least about 50%, about 60%, about 70%, about 75%, about 80%, about 85%, about 90%, about 92%, about 95%, about 96%, about 97%, about 98%, or about 99% proteolytic activity after exposure to altered temperatures over a given time period, for example, at least about 60 minutes, about 120 minutes, about 180 minutes, about 240 minutes, about 300 minutes, etc.
[0158] The term "enhanced stability" in the context of an oxidation, chelator, thermal, chemical, autolytic and/or pH stable protease refers to a higher retained proteolytic activity over time as compared to other proteases (e.g., thermolysin proteases) and/or wild-type enzymes.
[0159] The term "diminished stability" in the context of an oxidation, chelator, thermal and/or pH stable protease refers to a lower retained proteolytic activity over time as compared to other proteases (e.g., thermolysin proteases) and/or wild-type enzymes.
[0160] The term "cleaning activity" refers to a cleaning performance achieved by a metalloprotease polypeptide or reference protease under conditions prevailing during the proteolytic, hydrolyzing, cleaning, or other process of the invention. In some embodiments, cleaning performance of a metalloprotease polypeptide or reference protease may be determined by using various assays for cleaning one or more various enzyme sensitive stains on an item or surface (e.g., a stain resulting from food, grass, blood, ink, milk, oil, and/or egg protein). Cleaning performance of a variant or reference protease can be determined by subjecting the stain on the item or surface to standard wash condition(s) and assessing the degree to which the stain is removed by using various chromatographic, spectrophotometric, or other quantitative methodologies. Exemplary cleaning assays and methods are known in the art and include, but are not limited to those described in WO 99/34011 and U.S. Pat. No. 6,605,458, both of which are herein incorporated by reference, as well as those cleaning assays and methods included in the Examples provided below.
[0161] The term "cleaning effective amount" of a metalloprotease polypeptide or reference protease refers to the amount of protease that achieves a desired level of enzymatic activity in a specific cleaning composition. Such effective amounts are readily ascertained by one of ordinary skill in the art and are based on many factors, such as the particular protease used, the cleaning application, the specific composition of the cleaning composition, and whether a liquid or dry (e.g., granular, tablet, bar) composition is required, etc.
[0162] The term "cleaning adjunct material" refers to any liquid, solid, or gaseous material included in cleaning composition other than a metalloprotease polypeptide of the invention. In some embodiments, the cleaning compositions of the present invention include one or more cleaning adjunct materials. Each cleaning adjunct material is typically selected depending on the particular type and form of cleaning composition (e.g., liquid, granule, powder, bar, paste, spray, tablet, gel, foam, or other composition). Preferably, each cleaning adjunct material is compatible with the protease enzyme used in the composition.
[0163] The term "enhanced performance" in the context of cleaning activity refers to an increased or greater cleaning activity by an enzyme with respect to a parent or reference protein as measured on certain enzyme sensitive stains such as egg, milk, grass, ink, oil, and/or blood, as determined by usual evaluation after a standard wash cycle and/or multiple wash cycles.
[0164] The term "diminished performance" in the context of cleaning activity refers to a decreased or lesser cleaning activity by an enzyme on certain enzyme sensitive stains such as egg, milk, grass or blood, as determined by usual evaluation after a standard wash cycle and/or multiple wash cycles.
[0165] Cleaning compositions and cleaning formulations include any composition that is suited for cleaning, bleaching, disinfecting, and/or sterilizing any object, item, and/or surface. Such compositions and formulations include, but are not limited to for example, liquid and/or solid compositions, including cleaning or detergent compositions (e.g., liquid, tablet, gel, bar, granule, and/or solid laundry cleaning or detergent compositions and fine fabric detergent compositions; hard surface cleaning compositions and formulations, such as for glass, wood, ceramic and metal counter tops and windows; carpet cleaners; oven cleaners; fabric fresheners; fabric softeners; and textile, laundry booster cleaning or detergent compositions, laundry additive cleaning compositions, and laundry pre-spotter cleaning compositions; dishwashing compositions, including hand or manual dishwash compositions (e.g., "hand" or "manual" dishwashing detergents) and automatic dishwashing compositions (e.g., "automatic dishwashing detergents").
[0166] Cleaning composition or cleaning formulations, as used herein, include, unless otherwise indicated, granular or powder-form all-purpose or heavy-duty washing agents, especially cleaning detergents; liquid, granular, gel, solid, tablet, or paste-form all-purpose washing agents, especially the so-called heavy-duty liquid (HDL) detergent or heavy-duty powder detergent (HDD) types; liquid fine-fabric detergents; hand or manual dishwashing agents, including those of the high-foaming type; hand or manual dishwashing, automatic dishwashing, or dishware or tableware washing agents, including the various tablet, powder, solid, granular, liquid, gel, and rinse-aid types for household and institutional use; liquid cleaning and disinfecting agents, including antibacterial hand-wash types, cleaning bars, mouthwashes, denture cleaners, car shampoos, carpet shampoos, bathroom cleaners; hair shampoos and/or hair-rinses for humans and other animals; shower gels and foam baths and metal cleaners; as well as cleaning auxiliaries, such as bleach additives and "stain-stick" or pre-treat types. In some embodiments, granular compositions are in "compact" form; in some embodiments, liquid compositions are in a "concentrated" form.
[0167] As used herein, "fabric cleaning compositions" include hand and machine laundry detergent compositions including laundry additive compositions and compositions suitable for use in the soaking and/or pretreatment of stained fabrics (e.g., clothes, linens, and other textile materials).
[0168] As used herein, "non-fabric cleaning compositions" include non-textile (i.e., non-fabric) surface cleaning compositions, including, but not limited to for example, hand or manual or automatic dishwashing detergent compositions, oral cleaning compositions, denture cleaning compositions, and personal cleansing compositions.
[0169] As used herein, the term "fabric and/or hard surface cleaning and/or treatment composition" is a subset of cleaning and treatment compositions that includes, unless otherwise indicated, granular or powder-form all-purpose or "heavy-duty" washing agents, especially cleaning detergents; liquid, gel or paste-form all-purpose washing agents, especially the so-called heavy-duty liquid types; liquid fine-fabric detergents; hand dishwashing agents or light duty dishwashing agents, especially those of the high-foaming type; machine dishwashing agents, including the various tablet, granular, liquid and rinse-aid types for household and institutional use; liquid cleaning and disinfecting agents, car or carpet shampoos, bathroom cleaners including toilet bowl cleaners; fabric conditioning products including softening and/or freshening that may be in liquid, solid and/or dryer sheet form; as well as cleaning auxiliaries such as bleach additives and "stain-stick" or pre-treat types, substrate-laden products such as dryer added sheets. All of such products which are applicable may be in standard, concentrated or even highly concentrated form even to the extent that such products may in certain aspect be non-aqueous.
[0170] As used herein, the term "detergent composition" or "detergent formulation" is used in reference to a composition intended for use in a wash medium for the cleaning of soiled or dirty objects, including particular fabric and/or non-fabric objects or items. Such compositions of the present invention are not limited to any particular detergent composition or formulation. Indeed, in some embodiments, the detergents of the invention comprise at least one metalloprotease polypeptide of the invention and, in addition, one or more surfactants, transferase(s), hydrolytic enzymes, oxido reductases, builders (e.g., a builder salt), bleaching agents, bleach activators, bluing agents, fluorescent dyes, caking inhibitors, masking agents, enzyme activators, antioxidants, and/or solubilizers. In some instances, a builder salt is a mixture of a silicate salt and a phosphate salt, preferably with more silicate (e.g., sodium metasilicate) than phosphate (e.g., sodium tripolyphosphate). Some compositions of the invention, such as, but not limited to, cleaning compositions or detergent compositions, do not contain any phosphate (e.g., phosphate salt or phosphate builder).
[0171] As used herein, the term "bleaching" refers to the treatment of a material (e.g., fabric, laundry, pulp, etc.) or surface for a sufficient length of time and/or under appropriate pH and/or temperature conditions to effect a brightening (i.e., whitening) and/or cleaning of the material. Examples of chemicals suitable for bleaching include, but are not limited to, for example, ClO2, H2O2, peracids, NO2, etc.
[0172] As used herein, "wash performance" of a protease (e.g., a metalloprotease polypeptide of the invention) refers to the contribution of a metalloprotease polypeptide to washing that provides additional cleaning performance to the detergent as compared to the detergent without the addition of the metalloprotease polypeptide to the composition. Wash performance is compared under relevant washing conditions. In some test systems, other relevant factors, such as detergent composition, sud concentration, water hardness, washing mechanics, time, pH, and/or temperature, can be controlled in such a way that condition(s) typical for household application in a certain market segment (e g, hand or manual dishwashing, automatic dishwashing, dishware cleaning, tableware cleaning, fabric cleaning, etc.) are imitated.
[0173] The term "relevant washing conditions" is used herein to indicate the conditions, particularly washing temperature, time, washing mechanics, sud concentration, type of detergent and water hardness, actually used in households in a hand dishwashing, automatic dishwashing, or laundry detergent market segment.
[0174] The term "improved wash performance" is used to indicate that a better end result is obtained in stain removal under relevant washing conditions, or that less metalloprotease polypeptide, on weight basis, is needed to obtain the same end result relative to the corresponding wild-type or starting parent protease.
[0175] As used herein, the term "disinfecting" refers to the removal of contaminants from the surfaces, as well as the inhibition or killing of microbes on the surfaces of items. It is not intended that the present invention be limited to any particular surface, item, or contaminant(s) or microbes to be removed.
[0176] The "compact" form of the cleaning compositions herein is best reflected by density and, in terms of composition, by the amount of inorganic filler salt. Inorganic filler salts are conventional ingredients of detergent compositions in powder form. In conventional detergent compositions, the filler salts are present in substantial amounts, typically about 17 to about 35% by weight of the total composition. In contrast, in compact compositions, the filler salt is present in amounts not exceeding about 15% of the total composition. In some embodiments, the filler salt is present in amounts that do not exceed about 10%, or more preferably, about 5%, by weight of the composition. In some embodiments, the inorganic filler salts are selected from the alkali and alkaline-earth-metal salts of sulfates and chlorides. In some embodiments, the filler salt is sodium sulfate.
[0177] As used herein in connection with a numerical value, the term "about" refers to a range of +/-0.5 of the numerical value, unless the term is otherwise specifically defined in context. For instance, the phrase a "pH value of about 6" refers to pH values of from 5.5 to 6.5, unless the pH value is specifically defined otherwise.
[0178] The position of an amino acid residue in a given amino acid sequence is typically numbered herein using the numbering of the position of the corresponding amino acid residue of the wild type Paenibacillus metalloprotease amino acid sequences shown in SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38. The Paenibacillus sp. metalloprotease amino acid sequences, thus serves as a reference parent sequence. A given amino acid sequence, such as a metalloprotease enzyme amino acid sequence and variants thereof described herein, can be aligned with the wild type metalloprotease sequence (e.g., SEQ ID NO: 3) using an alignment algorithm as described herein, and an amino acid residue in the given amino acid sequence that aligns (preferably optimally aligns) with an amino acid residue in the wild type sequence can be conveniently numbered by reference to the corresponding amino acid residue in the metalloprotease sequence.
[0179] Oligonucleotide synthesis and purification steps are typically performed according to specifications. Techniques and procedures are generally performed according to conventional methods well known in the art and various general references that are provided throughout this document. Procedures therein are believed to be well known to those of ordinary skill in the art and are provided for the convenience of the reader.
Metalloprotease Polypeptides of the Present Invention
[0180] The present invention provides novel metalloprotease enzyme polypeptides, which may be collectively referred to as "enzymes of the invention" or "polypeptides of the invention." Polypeptides of the invention include isolated, recombinant, substantially pure, or non-naturally occurring polypeptides. In some embodiments, polypeptides of the invention are useful in cleaning applications and can be incorporated into cleaning compositions that are useful in methods of cleaning an item or a surface in need of cleaning.
[0181] In some embodiments, the enzyme of the present invention has 50, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38. In various embodiments, the enzyme of the present invention has 50, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to a metalloprotease enzyme from any genus in Tables 1.2, 2.2, 3.2, 4.2, 5.2, 6.2, 7.2 or 8.2.
[0182] In some embodiments, the enzyme of the present invention, including all embodiments supra, can be derived from a member of the order Bacillales or family Bacillaceae, Paenibacillaceae, Alicyclobacillaceae, or Lactobacillaceae. In some embodiments, the enzyme of the present invention, including all embodiments supra, can be derived from a Bacillus, Alicyclobacillus, Geobacillus, Exiguobacterium, Lactobacillus, or Paenibacillus species. In some embodiments, the enzyme of the present invention, including all embodiments supra, can be derived from a member of the Pseudococcidae family. In some embodiments, the enzyme of the present invention, including all embodiments supra, can be derived from a Planococcus species. Various enzyme metalloproteases have been found that have a high identity to each other and to the Paenibacillus enzymes as shown in SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38.
[0183] In a particular embodiment, the invention is an enzyme derived from the genus Paenibacillus. In a particular embodiment, the invention is an enzyme derived from the genus Paenibacillus and from the species Paenibacillus sp., Paenibacillus ehimensis, Paenibacillus hunanensis, Paenibacillus barcinonensis, Paenibacillus amylolyticus, Paenibacillus humicus and Paenibacillus polymyxa.
[0184] Described are compositions and methods relating to enzymes cloned from Paenibacillus. The compositions and methods are based, in part, on the observation that cloned and expressed enzymes of the present invention have proteolytic activity in the presence of a detergent composition. Enzymes of the present invention also demonstrate excellent stability in detergent compositions. These features makes enzymes of the present invention well suited for use in a variety of cleaning applications, where the enzyme can hydrolyze proteins in the presence of surfactants and other components found in detergent compositions.
[0185] In some embodiments, the invention includes an isolated, recombinant, substantially pure, or non-naturally occurring enzyme having protease activity, which polypeptide comprises a polypeptide sequence having at least about 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence identity to a parent enzyme as provided herein.
[0186] In some embodiments, the polypeptide of the present invention, is a polypeptide having a specified degree of amino acid sequence homology to the exemplified polypeptides, e.g., at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or even at least 99% sequence homology to the amino acid sequences of SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38. Homology can be determined by amino acid sequence alignment, e.g., using a program such as BLAST, ALIGN, or CLUSTAL, as described herein.
[0187] Also provided are polypeptide enzymes of the present invention, having protease activity, said enzymes comprising an amino acid sequence which differs from the amino acid sequence of SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38 by no more than 50, no more than 40, no more than 30, no more than 35, no more than 25, no more than 20, no more than 19, no more than 18, no more than 17, no more than 16, no more than 15, no more than 14, no more than 13, no more than 12, no more than 11, no more than 10, no more than 9, no more than 8, no more than 7, no more than 6, no more than 5, no more than 4, no more than 3, no more than 2, or no more than 1 amino acid residue(s), when aligned using any of the previously described alignment methods.
[0188] As noted above, the variant enzyme polypeptides of the invention have enzymatic activities (e.g., protease activities) and thus are useful in cleaning applications, including but not limited to, methods for cleaning dishware items, tableware items, fabrics, and items having hard surfaces (e.g., the hard surface of a table, table top, wall, furniture item, floor, ceiling, etc.). Exemplary cleaning compositions comprising one or more variant metalloprotease enzyme polypeptides of the invention are described infra. The enzymatic activity (e.g., protease enzyme activity) of an enzyme polypeptide of the invention can be determined readily using procedures well known to those of ordinary skill in the art. The Examples presented infra describe methods for evaluating the enzymatic activity and cleaning performance. The performance of polypeptide enzymes of the invention in removing stains (e.g., a protein stain such as blood/milk/ink or egg yolk), cleaning hard surfaces, or cleaning laundry, dishware or tableware item(s) can be readily determined using procedures well known in the art and/or by using procedures set forth in the Examples.
[0189] The metalloprotease polypeptides of the invention have protease activity such that they are useful in casein hydrolysis, collagen hydrolysis, elastin hydrolysis, keratin hydrolysis, soy protein hydrolysis or corn meal protein hydrolysis. Thus, the polypeptides of the invention find use in other applications such as pretreatments for food, feed, or protein degradation.
[0190] The polypeptides of the invention are also useful in pretreatment of animal feed products, such as soy protein, corn meal, and other protein rich components. Pretreatment of these animal feed products with a polypeptide of the invention may help in the breakdown of complex proteins into their hydrolysates which are easily digestible by animals.
[0191] In yet other embodiments, the disclosed metalloprotease polypeptides find use in hydrolysis of corn soy protein. The disclosed metalloprotease polypeptides may be used alone or in combination with other proteases, amylases or lipases to produce peptides and free amino acids from the corn or soy protein. In some embodiments, the recovered proteins, peptides or amino acids can be subsequently used in animal feed or human food products.
[0192] The polypeptides of the invention are also useful in treatment of wounds, particularly in wound debridement. Wound debridement is the removal of dead, damaged or infected tissue to improve the healing potential of the remaining healthy tissue. Debridement is an important part of the healing process for burns and other serious wounds. The wounds or burns may be treated with a composition comprising a polypeptide of the invention which would result in removal of unwanted damaged tissue and improving the healthy tissue.
The metalloprotease polypeptides of the present invention can have protease activity over a broad range of pH conditions. In some embodiments, the metalloprotease polypeptides have protease activity on azo-casein as a substrate, as demonstrated in Examples 3.1 to 3.8. In some embodiments, the metalloprotease polypeptides have protease activity at a pH of from about 3.0 to about 12.0. In some embodiments, the metalloprotease polypeptides have protease activity at a pH of from about 4.0 to about 10.5. In some embodiments, the metalloprotease polypeptides have at least 70% of maximal protease activity at a pH of from about 5.5 to about 9.0. In some embodiments, the metalloprotease polypeptides have at least 80% of maximal protease activity at a pH of from about 6.0 to about 8.5. In some embodiments, the metalloprotease polypeptides have maximal protease activity at a pH of about 7.5.
[0193] In some embodiments, the metalloprotease polypeptides of the present invention have protease activity at a temperature range of from about 10° C. to about 100° C. In some embodiments, the metalloprotease polypeptides of the present invention have protease activity at a temperature range of from about 20° C. to about 90° C. In some embodiments, the metalloprotease polypeptides have at least 70% of maximal protease activity at a temperature of from about 45° C. to about 60° C. In some embodiments, the metalloprotease polypeptides have maximal protease activity at a temperature of 50° C.
[0194] In some embodiments, the metalloprotease polypeptides of the present invention demonstrate cleaning performance in a cleaning composition. Cleaning compositions often include ingredients harmful to the stability and performance of enzymes, making cleaning compositions a harsh environment for enzymes, e.g. metalloproteases, to retain function. Thus, it is not trivial for an enzyme to be put in a cleaning composition and expect enzymatic function (e.g. metalloprotease activity, such as demonstrated by cleaning performance). In some embodiments, the metalloprotease polypeptides of the present invention demonstrate cleaning performance in automatic dishwashing (ADW) detergent compositions. In some embodiments, the cleaning performance in automatic dishwashing (ADW) detergent compositions includes cleaning of egg yolk stains. In some embodiments, the metalloprotease polypeptides of the present invention demonstrate cleaning performance in laundry detergent compositions. In some embodiments, the cleaning performance in laundry detergent compositions includes cleaning of blood/milk/ink stains. In each of the cleaning compositions, the metalloprotease polypeptides of the present invention demonstrate cleaning performance with or without a bleach component.
[0195] The metalloprotease polypeptides of the invention have protease activity such that they are useful in casein hydrolysis, collagen hydrolysis, elastin hydrolysis, keratin hydrolysis, soy protein hydrolysis or corn meal protein hydrolysis. Thus, the polypeptides of the invention find use in other applications such as pretreatments for food, feed, or protein degradation.
[0196] A polypeptide of the invention can be subject to various changes, such as one or more amino acid insertions, deletions, and/or substitutions, either conservative or non-conservative, including where such changes do not substantially alter the enzymatic activity of the polypeptide. Similarly, a nucleic acid of the invention can also be subject to various changes, such as one or more substitutions of one or more nucleotides in one or more codons such that a particular codon encodes the same or a different amino acid, resulting in either a silent variation (e.g., when the encoded amino acid is not altered by the nucleotide mutation) or non-silent variation, one or more deletions of one or more nucleic acids (or codons) in the sequence, one or more additions or insertions of one or more nucleic acids (or codons) in the sequence, and/or cleavage of or one or more truncations of one or more nucleic acids (or codons) in the sequence. Many such changes in the nucleic acid sequence may not substantially alter the enzymatic activity of the resulting encoded polypeptide enzyme compared to the polypeptide enzyme encoded by the original nucleic acid sequence. A nucleic acid sequence of the invention can also be modified to include one or more codons that provide for optimum expression in an expression system (e.g., bacterial expression system), while, if desired, said one or more codons still encode the same amino acid(s).
[0197] In some embodiments, the present invention provides a genus of enzyme polypeptides having the desired enzymatic activity (e.g., protease enzyme activity or cleaning performance activity) which comprise sequences having the amino acid substitutions described herein and also which comprise one or more additional amino acid substitutions, such as conservative and non-conservative substitutions, wherein the polypeptide exhibits, maintains, or approximately maintains the desired enzymatic activity (e.g., proteolytic activity, as reflected in the cleaning activity or performance of the polypeptide enzymes of SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 and 38). Amino acid substitutions in accordance with the invention may include, but are not limited to, one or more non-conservative substitutions and/or one or more conservative amino acid substitutions. A conservative amino acid residue substitution typically involves exchanging a member within one functional class of amino acid residues for a residue that belongs to the same functional class (conservative amino acid residues are considered functionally homologous or conserved in calculating percent functional homology). A conservative amino acid substitution typically involves the substitution of an amino acid in an amino acid sequence with a functionally similar amino acid. For example, alanine, glycine, serine, and threonine are functionally similar and thus may serve as conservative amino acid substitutions for one another. Aspartic acid and glutamic acid may serve as conservative substitutions for one another. Asparagine and glutamine may serve as conservative substitutions for one another. Arginine, lysine, and histidine may serve as conservative substitutions for one another. Isoleucine, leucine, methionine, and valine may serve as conservative substitutions for one another. Phenylalanine, tyrosine, and tryptophan may serve as conservative substitutions for one another.
[0198] Other conservative amino acid substitution groups can be envisioned. For example, amino acids can be grouped by similar function or chemical structure or composition (e.g., acidic, basic, aliphatic, aromatic, sulfur-containing). For instance, an aliphatic grouping may comprise: Glycine (G), Alanine (A), Valine (V), Leucine (L), Isoleucine (I). Other groups containing amino acids that are considered conservative substitutions for one another include: aromatic: Phenylalanine (F), Tyrosine (Y), Tryptophan (W); sulfur-containing: Methionine (M), Cysteine (C); Basic: Arginine (R), Lysine (K), Histidine (H); Acidic: Aspartic acid (D), Glutamic acid (E); non-polar uncharged residues, Cysteine (C), Methionine (M), and Proline (P); hydrophilic uncharged residues: Serine (S), Threonine (T), Asparagine (N), and Glutamine (Q). Additional groupings of amino acids are well-known to those of skill in the art and described in various standard textbooks. Listing of a polypeptide sequence herein, in conjunction with the above substitution groups, provides an express listing of all conservatively substituted polypeptide sequences.
[0199] More conservative substitutions exist within the amino acid residue classes described above, which also or alternatively can be suitable. Conservation groups for substitutions that are more conservative include: valine-leucine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alanine-valine, and asparagine-glutamine.
[0200] Conservatively substituted variations of a polypeptide sequence of the invention (e.g., variant metalloproteases of the invention) include substitutions of a small percentage, sometimes less than 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, or 6% of the amino acids of the polypeptide sequence, or less than 5%, 4%, 3%, 2%, or 1%, or less than 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 amino acid substitution of the amino acids of the polypeptide sequence, with a conservatively selected amino acid of the same conservative substitution group.
[0201] As described elsewhere herein in greater detail and in the Examples provided herein, polypeptides of the invention may have cleaning abilities that may be compared to known proteases, including known metalloproteases.
Nucleic Acids of the Invention
[0202] The invention provides isolated, non-naturally occurring, or recombinant nucleic acids which may be collectively referred to as "nucleic acids of the invention" or "polynucleotides of the invention", which encode polypeptides of the invention. Nucleic acids of the invention, including all described below, are useful in recombinant production (e.g., expression) of polypeptides of the invention, typically through expression of a plasmid expression vector comprising a sequence encoding the polypeptide of interest or fragment thereof. As discussed above, polypeptides include metalloprotease polypeptides having enzymatic activity (e.g., proteolytic activity) which are useful in cleaning applications and cleaning compositions for cleaning an item or a surface (e.g., surface of an item) in need of cleaning.
[0203] In some embodiments, the invention provides an isolated, recombinant, substantially pure, or non-naturally occurring nucleic acid comprising a nucleotide sequence encoding any polypeptide (including any fusion protein, etc.) of the invention described above in the section entitled "Polypeptides of the Invention" and elsewhere herein. The invention also provides an isolated, recombinant, substantially pure, or non-naturally-occurring nucleic acid comprising a nucleotide sequence encoding a combination of two or more of any polypeptides of the invention described above and elsewhere herein. In some embodiments, the nucleic acids of the present invention has 50, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to SEQ ID NO: 4, 9, 14, 19, 24, 29, 34 and 39.
[0204] The present invention provides nucleic acids encoding a metalloprotease polypeptide of the present invention, wherein the metalloprotease polypeptide is a mature form having proteolytic activity, wherein the amino acid positions of the variant are numbered by correspondence with the amino acid sequence of Paenibacillus metalloprotease polypeptides set forth as SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 or 38.
[0205] Nucleic acids of the invention can be generated by using any suitable synthesis, manipulation, and/or isolation techniques, or combinations thereof. For example, a polynucleotide of the invention may be produced using standard nucleic acid synthesis techniques, such as solid-phase synthesis techniques that are well-known to those skilled in the art. In such techniques, fragments of up to 50 or more nucleotide bases are typically synthesized, then joined (e.g., by enzymatic or chemical ligation methods) to form essentially any desired continuous nucleic acid sequence. The synthesis of the nucleic acids of the invention can be also facilitated by any suitable method known in the art, including but not limited to chemical synthesis using the classical phosphoramidite method (See e.g., Beaucage et al. Tetrahedron Letters 22:1859-69
[1981]); or the method described by Matthes et al. (See, Matthes et al., EMBO J. 3:801-805
[1984], as is typically practiced in automated synthetic methods. Nucleic acids of the invention also can be produced by using an automatic DNA synthesizer. Customized nucleic acids can be ordered from a variety of commercial sources (e.g., The Midland Certified Reagent Company, the Great American Gene Company, Operon Technologies Inc., and DNA2.0). Other techniques for synthesizing nucleic acids and related principles are known in the art (See e.g., Itakura et al., Ann Rev. Biochem. 53:323
[1984]; and Itakura et al., Science 198:1056
[1984]).
[0206] As indicated above, recombinant DNA techniques useful in modification of nucleic acids are well known in the art. For example, techniques such as restriction endonuclease digestion, ligation, reverse transcription and cDNA production, and polymerase chain reaction (e.g., PCR) are known and readily employed by those of skill in the art. Nucleotides of the invention may also be obtained by screening cDNA libraries using one or more oligonucleotide probes that can hybridize to or PCR-amplify polynucleotides which encode a metalloprotease polypeptide polypeptide(s) of the invention. Procedures for screening and isolating cDNA clones and PCR amplification procedures are well known to those of skill in the art and described in standard references known to those skilled in the art. Some nucleic acids of the invention can be obtained by altering a naturally occurring polynucleotide backbone (e.g., that encodes an enzyme or parent protease) by, for example, a known mutagenesis procedure (e.g., site-directed mutagenesis, site saturation mutagenesis, and in vitro recombination).
Methods for Making Modified Metalloprotease polypeptides of the Invention
[0207] A variety of methods are known in the art that are suitable for generating modified polynucleotides of the invention that encode metalloprotease polypeptides of the invention, including, but not limited to, for example, site-saturation mutagenesis, scanning mutagenesis, insertional mutagenesis, deletion mutagenesis, random mutagenesis, site-directed mutagenesis, and directed-evolution, as well as various other recombinatorial approaches. Methods for making modified polynucleotides and proteins (e.g., metalloprotease polypeptides) include DNA shuffling methodologies, methods based on non-homologous recombination of genes, such as ITCHY (See, Ostermeier et al., 7:2139-44
[1999]), SCRACHY (See, Lutz et al. 98:11248-53
[2001]), SHIPREC (See, Sieber et al., 19:456-60
[2001]), and NRR (See, Bittker et al., 20:1024-9
[2001]; Bittker et al., 101:7011-6
[2004]), and methods that rely on the use of oligonucleotides to insert random and targeted mutations, deletions and/or insertions (See, Ness et al., 20:1251-5
[2002]; Coco et al., 20:1246-50
[2002]; Zha et al., 4:34-9
[2003]; Glaser et al., 149:3903-13
[1992]).
Vectors, Cells, and Methods for Producing Metalloprotease Polypeptides of the Invention
[0208] The present invention provides vectors comprising at least one metalloprotease polynucleotide of the invention described herein (e.g., a polynucleotide encoding a metalloprotease polypeptide of the invention described herein), expression vectors or expression cassettes comprising at least one nucleic acid or polynucleotide of the invention, isolated, substantially pure, or recombinant DNA constructs comprising at least one nucleic acid or polynucleotide of the invention, isolated or recombinant cells comprising at least one polynucleotide of the invention, and compositions comprising one or more such vectors, nucleic acids, expression vectors, expression cassettes, DNA constructs, cells, cell cultures, or any combination or mixtures thereof.
[0209] In some embodiments, the invention provides recombinant cells comprising at least one vector (e.g., expression vector or DNA construct) of the invention which comprises at least one nucleic acid or polynucleotide of the invention. Some such recombinant cells are transformed or transfected with such at least one vector. Such cells are typically referred to as host cells. Some such cells comprise bacterial cells, including, but are not limited to Bacillus sp. cells, such as B. subtilis cells. The invention also provides recombinant cells (e.g., recombinant host cells) comprising at least one metalloprotease polypeptide of the invention.
[0210] In some embodiments, the invention provides a vector comprising a nucleic acid or polynucleotide of the invention. In some embodiments, the vector is an expression vector or expression cassette in which a polynucleotide sequence of the invention which encodes a metalloprotease polypeptide of the invention is operably linked to one or additional nucleic acid segments required for efficient gene expression (e.g., a promoter operably linked to the polynucleotide of the invention which encodes a metalloprotease polypeptide of the invention). A vector may include a transcription terminator and/or a selection gene, such as an antibiotic resistance gene, that enables continuous cultural maintenance of plasmid-infected host cells by growth in antimicrobial-containing media.
[0211] An expression vector may be derived from plasmid or viral DNA, or in alternative embodiments, contains elements of both. Exemplary vectors include, but are not limited to pC194, pJH101, pE194, pHP13 (See, Harwood and Cutting [eds.], Chapter 3, Molecular Biological Methods for Bacillus, John Wiley & Sons
[1990]; suitable replicating plasmids for B. subtilis include those listed on p. 92) See also, Perego, Integrational Vectors for Genetic Manipulations in Bacillus subtilis, in Sonenshein et al., [eds.] Bacillus subtilis and Other Gram-Positive Bacteria: Biochemistry, Physiology and Molecular Genetics, American Society for Microbiology, Washington, D.C.
[1993], pp. 615-624), and p2JM103BBI.
[0212] For expression and production of a protein of interest (e.g., metalloprotease polypeptide) in a cell, at least one expression vector comprising at least one copy of a polynucleotide encoding the metalloprotease polypeptide, and in some instances comprising multiple copies, is transformed into the cell under conditions suitable for expression of the metalloprotease. In some embodiments of the present invention, a polynucleotide sequence encoding the metalloprotease polypeptide (as well as other sequences included in the vector) is integrated into the genome of the host cell, while in other embodiments, a plasmid vector comprising a polynucleotide sequence encoding the metalloprotease polypeptide remains as autonomous extra-chromosomal element within the cell. The invention provides both extrachromosomal nucleic acid elements as well as incoming nucleotide sequences that are integrated into the host cell genome. The vectors described herein are useful for production of the metalloprotease polypeptides of the invention. In some embodiments, a polynucleotide construct encoding the metalloprotease polypeptide is present on an integrating vector that enables the integration and optionally the amplification of the polynucleotide encoding the metalloprotease polypeptide into the host chromosome. Examples of sites for integration are well known to those skilled in the art. In some embodiments, transcription of a polynucleotide encoding a metalloprotease polypeptide of the invention is effectuated by a promoter that is the wild-type promoter for the selected precursor protease. In some other embodiments, the promoter is heterologous to the precursor protease, but is functional in the host cell. Specifically, examples of suitable promoters for use in bacterial host cells include, but are not limited to, for example, the amyE, amyQ, amyL, pstS, sacB, pSPAC, pAprE, pVeg, pHpaII promoters, the promoter of the B. stearothermophilus maltogenic amylase gene, the B. amyloliquefaciens (BAN) amylase gene, the B. subtilis alkaline protease gene, the B. clausii alkaline protease gene the B. pumilis xylosidase gene, the B. thuringiensis cryIIIA, and the B. licheniformis alpha-amylase gene. Additional promoters include, but are not limited to the A4 promoter, as well as phage Lambda PR or PL promoters, and the E. coli lac, trp or tac promoters.
[0213] Metalloprotease polypeptides of the present invention can be produced in host cells of any suitable microorganism, including bacteria and fungi. In some embodiments, metalloprotease polypeptides of the present invention can be produced in Gram-positive bacteria. In some embodiments, the host cells are Bacillus spp., Streptomyces spp., Escherichia spp., Aspergillus spp., Trichoderma spp., Pseudomonas spp., Corynebacterium spp., Saccharomyces spp., or Pichia spp. In some embodiments, the metalloprotease polypeptides are produced by Bacillus sp. host cells. Examples of Bacillus sp. host cells that find use in the production of the metalloprotease polypeptides of the invention include, but are not limited to B. licheniformis, B. lentus, B. subtilis, B. amyloliquefaciens, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. coagulans, B. circulans, B. pumilis, B. thuringiensis, B. clausii, and B. megaterium, as well as other organisms within the genus Bacillus. In some embodiments, B. subtilis host cells are used for production of metalloprotease polypeptides. U.S. Pat. Nos. 5,264,366 and 4,760,025 (RE 34,606) describe various Bacillus host strains that can be used for producing metalloprotease polypeptide of the invention, although other suitable strains can be used.
[0214] Several bacterial strains that can be used to produce metalloprotease polypeptides of the invention include non-recombinant (i.e., wild-type) Bacillus sp. strains, as well as variants of naturally-occurring strains and/or recombinant strains. In some embodiments, the host strain is a recombinant strain, wherein a polynucleotide encoding a polypeptide of interest has been introduced into the host. In some embodiments, the host strain is a B. subtilis host strain and particularly a recombinant Bacillus subtilis host strain. Numerous B. subtilis strains are known, including, but not limited to for example, 1A6 (ATCC 39085), 168 (1A01), SB19, W23, Ts85, B637, PB1753 through PB1758, PB3360, JH642, 1A243 (ATCC 39,087), ATCC 21332, ATCC 6051, MI113, DE100 (ATCC 39,094), GX4931, PBT 110, and PEP 211strain (See e.g., Hoch et al., Genetics 73:215-228
[1973]; See also, U.S. Pat. Nos. 4,450,235 and 4,302,544, and EP 0134048, each of which is incorporated by reference in its entirety). The use of B. subtilis as an expression host cells is well known in the art (See e.g., Palva et al., Gene 19:81-87
[1982]; Fahnestock and Fischer, J. Bacteriol., 165:796-804
[1986]; and Wang et al., Gene 69:39-47
[1988]).
[0215] In some embodiments, the Bacillus host cell is a Bacillus sp. that includes a mutation or deletion in at least one of the following genes, degU, degS, degR and degQ. In some embodiments, the mutation is in a degU gene, and in some embodiments the mutation is degU(Hy)32 (See e.g., Msadek et al., J. Bacteriol. 172:824-834
[1990]; and Olmos et al., Mol. Gen. Genet. 253:562-567
[1997]). In some embodiments, the Bacillus host comprises a mutation or deletion in scoC4 (See e.g., Caldwell et al., J. Bacteriol. 183:7329-7340
[2001]); spoIIE (See e.g., Arigoni et al., Mol. Microbiol. 31:1407-1415
[1999]); and/or oppA or other genes of the opp operon (See e.g., Perego et al., Mol. Microbiol. 5:173-185
[1991]). Indeed, it is contemplated that any mutation in the opp operon that causes the same phenotype as a mutation in the oppA gene will find use in some embodiments of the altered Bacillus strain of the invention. In some embodiments, these mutations occur alone, while in other embodiments, combinations of mutations are present. In some embodiments, an altered Bacillus host cell strain that can be used to produce a metalloprotease polypeptide of the invention is a Bacillus host strain that already includes a mutation in one or more of the above-mentioned genes. In addition, Bacillus sp. host cells that comprise mutation(s) and/or deletions of endogenous protease genes find use. In some embodiments, the Bacillus host cell comprises a deletion of the aprE and the nprE genes. In other embodiments, the Bacillus sp. host cell comprises a deletion of 5 protease genes, while in other embodiments, the Bacillus sp. host cell comprises a deletion of 9 protease genes (See e.g., U.S. Pat. Appln. Pub. No. 2005/0202535, incorporated herein by reference).
[0216] Host cells are transformed with at least one nucleic acid encoding at least one metalloprotease polypeptide of the invention using any suitable method known in the art. Methods for introducing a nucleic acid (e.g., DNA) into Bacillus cells or E. coli cells utilizing plasmid DNA constructs or vectors and transforming such plasmid DNA constructs or vectors into such cells are well known. In some embodiments, the plasmids are subsequently isolated from E. coli cells and transformed into Bacillus cells. However, it is not essential to use intervening microorganisms such as E. coli, and in some embodiments, a DNA construct or vector is directly introduced into a Bacillus host.
[0217] Those of skill in the art are well aware of suitable methods for introducing nucleic acid sequences of the invention into Bacillus cells (See e.g., Ferrari et al., "Genetics," in Harwood et al. [eds.], Bacillus, Plenum Publishing Corp.
[1989], pp. 57-72; Saunders et al., J. Bacteriol. 157:718-726
[1984]; Hoch et al., J. Bacteriol. 93:1925-1937
[1967]; Mann et al., Current Microbiol. 13:131-135
[1986]; Holubova, Folia Microbiol. 30:97
[1985]; Chang et al., Mol. Gen. Genet. 168:11-115
[1979]; Vorobjeva et al., FEMS Microbiol. Lett. 7:261-263
[1980]; Smith et al., Appl. Env. Microbiol. 51:634
[1986]; Fisher et al., Arch. Microbiol. 139:213-217
[1981]; and McDonald, J. Gen. Microbiol. 130:203
[1984]). Indeed, such methods as transformation, including protoplast transformation and transfection, transduction, and protoplast fusion are well known and suited for use in the present invention. Methods known in the art to transform Bacillus cells include such methods as plasmid marker rescue transformation, which involves the uptake of a donor plasmid by competent cells carrying a partially homologous resident plasmid (See, Contente et al., Plasmid 2:555-571
[1979]; Haima et al., Mol. Gen. Genet. 223:185-191
[1990]; Weinrauch et al., J. Bacteriol. 154:1077-1087
[1983]; and Weinrauch et al., J. Bacteriol. 169:1205-1211
[1987]). In this method, the incoming donor plasmid recombines with the homologous region of the resident "helper" plasmid in a process that mimics chromosomal transformation.
[0218] In addition to commonly used methods, in some embodiments, host cells are directly transformed with a DNA construct or vector comprising a nucleic acid encoding a metalloprotease polypeptide of the invention (i.e., an intermediate cell is not used to amplify, or otherwise process, the DNA construct or vector prior to introduction into the host cell). Introduction of the DNA construct or vector of the invention into the host cell includes those physical and chemical methods known in the art to introduce a nucleic acid sequence (e.g., DNA sequence) into a host cell without insertion into the host genome. Such methods include, but are not limited to calcium chloride precipitation, electroporation, naked DNA, liposomes and the like. In additional embodiments, DNA constructs or vector are co-transformed with a plasmid, without being inserted into the plasmid. In further embodiments, a selective marker is deleted from the altered Bacillus strain by methods known in the art (See, Stahl et al., J. Bacteriol. 158:411-418
[1984]; and Palmeros et al., Gene 247:255-264
[2000]).
[0219] In some embodiments, the transformed cells of the present invention are cultured in conventional nutrient media. The suitable specific culture conditions, such as temperature, pH and the like are known to those skilled in the art and are well described in the scientific literature. In some embodiments, the invention provides a culture (e.g., cell culture) comprising at least one metalloprotease polypeptide or at least one nucleic acid of the invention.
[0220] In some embodiments, host cells transformed with at least one polynucleotide sequence encoding at least one metalloprotease polypeptide of the invention are cultured in a suitable nutrient medium under conditions permitting the expression of the present protease, after which the resulting protease is recovered from the culture. In some embodiments, the protease produced by the cells is recovered from the culture medium by conventional procedures, including, but not limited to for example, separating the host cells from the medium by centrifugation or filtration, precipitating the proteinaceous components of the supernatant or filtrate by means of a salt (e.g., ammonium sulfate), chromatographic purification (e.g., ion exchange, gel filtration, affinity, etc.).
[0221] In some embodiments, a metalloprotease polypeptide produced by a recombinant host cell is secreted into the culture medium. A nucleic acid sequence that encodes a purification facilitating domain may be used to facilitate purification of proteins. A vector or DNA construct comprising a polynucleotide sequence encoding a metalloprotease polypeptide may further comprise a nucleic acid sequence encoding a purification facilitating domain to facilitate purification of the metalloprotease polypeptide (See e.g., Kroll et al., DNA Cell Biol. 12:441-53
[1993]). Such purification facilitating domains include, but are not limited to, for example, metal chelating peptides such as histidine-tryptophan modules that allow purification on immobilized metals (See, Porath, Protein Expr. Purif. 3:263-281
[1992]), protein A domains that allow purification on immobilized immunoglobulin, and the domain utilized in the FLAGS extension/affinity purification system. The inclusion of a cleavable linker sequence such as Factor XA or enterokinase (e.g., sequences available from Invitrogen, San Diego, Calif.) between the purification domain and the heterologous protein also find use to facilitate purification.
[0222] Assays for detecting and measuring the enzymatic activity of an enzyme, such as a metalloprotease polypeptide of the invention, are well known. Various assays for detecting and measuring activity of proteases (e.g., metalloprotease polypeptides of the invention), are also known to those of ordinary skill in the art. In particular, assays are available for measuring protease activity that are based on the release of acid-soluble peptides from casein or hemoglobin, measured as absorbance at 280 nm or colorimetrically using the Folin method. Other exemplary assays involve the solubilization of chromogenic substrates (See e.g., Ward, "Proteinases," in Fogarty (ed.)., Microbial Enzymes and Biotechnology, Applied Science, London,
[1983], pp. 251-317). Other exemplary assays include, but are not limited to succinyl-Ala-Ala-Pro-Phe-para nitroanilide assay (suc-AAPF-pNA)(SEQ ID NO: 43) and the 2,4,6-trinitrobenzene sulfonate sodium salt assay (TNBS assay). Numerous additional references known to those in the art provide suitable methods (See e.g., Wells et al., Nucleic Acids Res. 11:7911-7925
[1983]; Christianson et al., Anal. Biochem. 223:119-129
[1994]; and Hsia et al., Anal Biochem. 242:221-227
[1999]).
[0223] A variety of methods can be used to determine the level of production of a mature protease (e.g., mature metalloprotease polypeptides of the present invention) in a host cell. Such methods include, but are not limited to, for example, methods that utilize either polyclonal or monoclonal antibodies specific for the protease. Exemplary methods include, but are not limited to enzyme-linked immunosorbent assays (ELISA), radioimmunoassays (RIA), fluorescent immunoassays (FIA), and fluorescent activated cell sorting (FACS). These and other assays are well known in the art (See e.g., Maddox et al., J. Exp. Med. 158:1211
[1983]).
[0224] In some other embodiments, the invention provides methods for making or producing a mature metalloprotease polypeptide of the invention. A mature metalloprotease polypeptide does not include a signal peptide or a propeptide sequence. Some methods comprise making or producing a metalloprotease polypeptide of the invention in a recombinant bacterial host cell, such as for example, a Bacillus sp. cell (e.g., a B. subtilis cell). In some embodiments, the invention provides a method of producing a metalloprotease polypeptide of the invention, the method comprising cultivating a recombinant host cell comprising a recombinant expression vector comprising a nucleic acid encoding a metalloprotease polypeptide of the invention under conditions conducive to the production of the metalloprotease polypeptide. Some such methods further comprise recovering the metalloprotease polypeptide from the culture.
[0225] In some embodiments the invention provides methods of producing a metalloprotease polypeptide of the invention, the methods comprising: (a) introducing a recombinant expression vector comprising a nucleic acid encoding a metalloprotease polypeptide of the invention into a population of cells (e.g., bacterial cells, such as B. subtilis cells); and (b) culturing the cells in a culture medium under conditions conducive to produce the metalloprotease polypeptide encoded by the expression vector. Some such methods further comprise: (c) isolating the metalloprotease polypeptide from the cells or from the culture medium.
Fabric and Home Care Products
[0226] In some embodiments, the metalloprotease polypeptides of the present invention can be used in compositions comprising an adjunct material and a metalloprotease polypeptide, wherein the composition is a fabric and home care product.
[0227] In some embodiments, the fabric and home care product compositions comprising at least one metalloprotease polypeptide comprise one or more of the following ingredients (based on total composition weight): from about 0.0005 wt % to about 0.1 wt %, from about 0.001 wt % to about 0.05 wt %, or even from about 0.002 wt % to about 0.03 wt % of said metalloprotease polypeptide; and one or more of the following: from about 0.00003 wt % to about 0.1 wt % fabric hueing agent; from about 0.001 wt % to about 5 wt %, perfume capsules; from about 0.001 wt % to about 1 wt %, cold-water soluble brighteners; from about 0.00003 wt % to about 0.1 wt % bleach catalysts; from about 0.00003 wt % to about 0.1 wt % first wash lipases; from about 0.00003 wt % to about 0.1 wt % bacterial cleaning cellulases; and/or from about 0.05 wt % to about 20 wt % Guerbet nonionic surfactants.
[0228] In some embodiments, the fabric and home care product composition is a liquid laundry detergent or a dishwashing detergent, such as an automatic dishwashing (ADW) detergent or hand dishwashing detergent.
[0229] It is intended that the fabric and home care product is provided in any suitable form, including a fluid or solid, or granular, powder, solid, bar, liquid, tablet, gel, or paste form. The fabric and home care product may be in the form of a unit dose pouch, especially when in the form of a liquid, and typically the fabric and home care product is at least partially, or even completely, enclosed by a water-soluble pouch. In addition, in some embodiments of the fabric and home care products comprising at least one metalloprotease polypeptide, the fabric and home care product may have any combination of parameters and/or characteristics detailed above.
Compositions Having the Metalloprotease Polypeptide of the Present Invention
[0230] Unless otherwise noted, all component or composition levels provided herein are made in reference to the active level of that component or composition, and are exclusive of impurities, for example, residual solvents or by-products, which may be present in commercially available sources. Enzyme components weights are based on total active protein. All percentages and ratios are calculated by weight unless otherwise indicated. All percentages and ratios are calculated based on the total composition unless otherwise indicated. Compositions of the invention include cleaning compositions, such as detergent compositions. In the exemplified detergent compositions, the enzymes levels are expressed by pure enzyme by weight of the total composition and unless otherwise specified, the detergent ingredients are expressed by weight of the total compositions.
[0231] As indicated herein, in some embodiments, the cleaning compositions of the present invention further comprise adjunct materials including, but not limited to, surfactants, builders, bleaches, bleach activators, bleach catalysts, other enzymes, enzyme stabilizing systems, chelants, optical brighteners, soil release polymers, dye transfer agents, dispersants, suds suppressors, dyes, perfumes, colorants, filler salts, hydrotropes, photoactivators, fluorescers, fabric conditioners, hydrolyzable surfactants, preservatives, anti-oxidants, anti-shrinkage agents, anti-wrinkle agents, germicides, fungicides, color speckles, silvercare, anti-tarnish and/or anti-corrosion agents, alkalinity sources, solubilizing agents, carriers, processing aids, pigments, and pH control agents (See e.g., U.S. Pat. Nos. 6,610,642, 6,605,458, 5,705,464, 5,710,115, 5,698,504, 5,695,679, 5,686,014 and 5,646,101, all of which are incorporated herein by reference). Embodiments of specific cleaning composition materials are exemplified in detail below. In embodiments in which the cleaning adjunct materials are not compatible with the metalloprotease polypeptides of the present invention in the cleaning compositions, then suitable methods of keeping the cleaning adjunct materials and the protease(s) separated (i.e., not in contact with each other) until combination of the two components is appropriate are used. Such separation methods include any suitable method known in the art (e.g., gelcaps, encapsulation, tablets, physical separation, etc.).
[0232] The cleaning compositions of the present invention are advantageously employed for example, in laundry applications, hard surface cleaning, dishwashing applications, including automatic dishwashing and hand dishwashing, as well as cosmetic applications such as dentures, teeth, hair and skin. In addition, due to the unique advantages of increased effectiveness in lower temperature solutions, the enzymes of the present invention are ideally suited for laundry applications. Furthermore, the enzymes of the present invention find use in granular and liquid compositions.
[0233] The metalloprotease polypeptides of the present invention also find use in cleaning additive products. In some embodiments, low temperature solution cleaning applications find use. In some embodiments, the present invention provides cleaning additive products including at least one enzyme of the present invention is ideally suited for inclusion in a wash process when additional bleaching effectiveness is desired. Such instances include, but are not limited to low temperature solution cleaning applications. In some embodiments, the additive product is in its simplest form, one or more proteases. In some embodiments, the additive is packaged in dosage form for addition to a cleaning process. In some embodiments, the additive is packaged in dosage form for addition to a cleaning process where a source of peroxygen is employed and increased bleaching effectiveness is desired. Any suitable single dosage unit form finds use with the present invention, including but not limited to pills, tablets, gelcaps, or other single dosage units such as pre-measured powders or liquids. In some embodiments, filler(s) or carrier material(s) are included to increase the volume of such compositions. Suitable filler or carrier materials include, but are not limited to, various salts of sulfate, carbonate and silicate as well as talc, clay and the like. Suitable filler or carrier materials for liquid compositions include, but are not limited to water or low molecular weight primary and secondary alcohols including polyols and diols. Examples of such alcohols include, but are not limited to, methanol, ethanol, propanol and isopropanol. In some embodiments, the compositions contain from about 5% to about 90% of such materials. Acidic fillers find use to reduce pH. Alternatively, in some embodiments, the cleaning additive includes adjunct ingredients, as more fully described below.
[0234] The present cleaning compositions and cleaning additives require an effective amount of at least one of the metalloprotease polypeptides provided herein, alone or in combination with other proteases and/or additional enzymes. The required level of enzyme is achieved by the addition of one or more metalloprotease polypeptides of the present invention. Typically the present cleaning compositions comprise at least about 0.0001 weight percent, from about 0.0001 to about 10, from about 0.001 to about 1, or from about 0.01 to about 0.1 weight percent of at least one of the metalloprotease polypeptides of the present invention.
[0235] The cleaning compositions herein are typically formulated such that, during use in aqueous cleaning operations, the wash water will have a pH of from about 4.0 to about 11.5, or even from about 5.0 to about 11.5, or even from about 5.0 to about 8.0, or even from about 7.5 to about 10.5. Liquid product formulations are typically formulated to have a pH from about 3.0 to about 9.0 or even from about 3 to about 5. Granular laundry products are typically formulated to have a pH from about 9 to about 11. Techniques for controlling pH at recommended usage levels include the use of buffers, alkalis, acids, etc., and are well known to those skilled in the art.
[0236] Suitable "low pH cleaning compositions" typically have a pH of from about 3 to about 5, and are typically free of surfactants that hydrolyze in such a pH environment. Such surfactants include sodium alkyl sulfate surfactants that comprise at least one ethylene oxide moiety or even from about 1 to about 16 moles of ethylene oxide. Such cleaning compositions typically comprise a sufficient amount of a pH modifier, such as sodium hydroxide, monoethanolamine or hydrochloric acid, to provide such cleaning composition with a pH of from about 3 to about 5. Such compositions typically comprise at least one acid stable enzyme. In some embodiments, the compositions are liquids, while in other embodiments, they are solids. The pH of such liquid compositions is typically measured as a neat pH. The pH of such solid compositions is measured as a 10% solids solution of said composition wherein the solvent is distilled water. In these embodiments, all pH measurements are taken at 20° C., unless otherwise indicated.
[0237] In some embodiments, when the metalloprotease polypeptide (s) is/are employed in a granular composition or liquid, it is desirable for the metalloprotease polypeptide to be in the form of an encapsulated particle to protect the metalloprotease polypeptide from other components of the granular composition during storage. In addition, encapsulation is also a means of controlling the availability of the metalloprotease polypeptide during the cleaning process. In some embodiments, encapsulation enhances the performance of the metalloprotease polypeptide (s) and/or additional enzymes. In this regard, the metalloprotease polypeptides of the present invention are encapsulated with any suitable encapsulating material known in the art. In some embodiments, the encapsulating material typically encapsulates at least part of the metalloprotease polypeptide (s) of the present invention. Typically, the encapsulating material is water-soluble and/or water-dispersible. In some embodiments, the encapsulating material has a glass transition temperature (Tg) of 0° C. or higher. Glass transition temperature is described in more detail in WO 97/11151. The encapsulating material is typically selected from consisting of carbohydrates, natural or synthetic gums, chitin, chitosan, cellulose and cellulose derivatives, silicates, phosphates, borates, polyvinyl alcohol, polyethylene glycol, paraffin waxes, and combinations thereof. When the encapsulating material is a carbohydrate, it is typically selected from monosaccharides, oligosaccharides, polysaccharides, and combinations thereof. In some typical embodiments, the encapsulating material is a starch (See e.g., EP 0 922 499; U.S. Pat. No. 4,977,252; U.S. Pat. No. 5,354,559, and U.S. Pat. No. 5,935,826). In some embodiments, the encapsulating material is a microsphere made from plastic such as thermoplastics, acrylonitrile, methacrylonitrile, polyacrylonitrile, polymethacrylonitrile and mixtures thereof; commercially available microspheres that find use include, but are not limited to those supplied by EXPANCEL® (Stockviksverken, Sweden), and PM 6545, PM 6550, PM 7220, PM 7228, EXTENDOSPHERES®, LUXSIL®, Q-CEL®, and SPHERICEL® (PQ Corp., Valley Forge, Pa.).
[0238] As described herein, the metalloprotease polypeptides of the present invention find particular use in the cleaning industry, including, but not limited to laundry and dish detergents. These applications place enzymes under various environmental stresses. The metalloprotease polypeptides of the present invention provide advantages over many currently used enzymes, due to their stability under various conditions.
[0239] Indeed, there are a variety of wash conditions including varying detergent formulations, wash water volumes, wash water temperatures, and lengths of wash time, to which proteases involved in washing are exposed. In addition, detergent formulations used in different geographical areas have different concentrations of their relevant components present in the wash water. For example, European detergents typically have about 4500-5000 ppm of detergent components in the wash water, while Japanese detergents typically have approximately 667 ppm of detergent components in the wash water. In North America, particularly the United States, detergents typically have about 975 ppm of detergent components present in the wash water.
[0240] A low detergent concentration system includes detergents where less than about 800 ppm of the detergent components are present in the wash water. Japanese detergents are typically considered low detergent concentration system as they have approximately 667 ppm of detergent components present in the wash water.
[0241] A medium detergent concentration includes detergents where between about 800 ppm and about 2000 ppm of the detergent components are present in the wash water. North American detergents are generally considered to be medium detergent concentration systems as they have approximately 975 ppm of detergent components present in the wash water. Brazil typically has approximately 1500 ppm of detergent components present in the wash water.
[0242] A high detergent concentration system includes detergents where greater than about 2000 ppm of the detergent components are present in the wash water. European detergents are generally considered to be high detergent concentration systems as they have approximately 4500-5000 ppm of detergent components in the wash water.
[0243] Latin American detergents are generally high suds phosphate builder detergents and the range of detergents used in Latin America can fall in both the medium and high detergent concentrations as they range from 1500 ppm to 6000 ppm of detergent components in the wash water. As mentioned above, Brazil typically has approximately 1500 ppm of detergent components present in the wash water. However, other high suds phosphate builder detergent geographies, not limited to other Latin American countries, may have high detergent concentration systems up to about 6000 ppm of detergent components present in the wash water.
[0244] In light of the foregoing, it is evident that concentrations of detergent compositions in typical wash solutions throughout the world varies from less than about 800 ppm of detergent to about 6000 ppm in high suds phosphate builder geographies.
[0245] The concentrations of the typical wash solutions are determined empirically. For example, in the U.S., a typical washing machine holds a volume of about 64.4 L of wash solution. Accordingly, in order to obtain a concentration of about 975 ppm of detergent within the wash solution about 62.79 g of detergent composition must be added to the 64.4 L of wash solution. This amount is the typical amount measured into the wash water by the consumer using the measuring cup provided with the detergent.
[0246] As a further example, different geographies use different wash temperatures. The temperature of the wash water in Japan is typically less than that used in Europe. For example, the temperature of the wash water in North America and Japan is typically between about 10 and about 40° C. (e.g., about 20° C.), whereas the temperature of wash water in Europe is typically between about 30 and about 60° C. (e.g., about 40° C.). However, in the interest of saving energy, many consumers are switching to using cold water washing. In addition, in some further regions, cold water is typically used for laundry, as well as dish washing applications. In some embodiments, the "cold water washing" of the present invention utilizes "cold water detergent" suitable for washing at temperatures from about 10° C. to about 40° C., or from about 20° C. to about 30° C., or from about 15° C. to about 25° C., as well as all other combinations within the range of about 15° C. to about 35° C., and all ranges within 10° C. to 40° C.
[0247] As a further example, different geographies typically have different water hardness. Water hardness is usually described in terms of the grains per gallon mixed Ca2+/Mg2+. Hardness is a measure of the amount of calcium (Ca2+) and magnesium (Mg2+) in the water. Most water in the United States is hard, but the degree of hardness varies. Moderately hard (60-120 ppm) to hard (121-181 ppm) water has 60 to 181 parts per million (parts per million converted to grains per U.S. gallon is ppm # divided by 17.1 equals grains per gallon) of hardness minerals.
TABLE-US-00001 Water Grains per gallon Parts per million Soft less than 1.0 less than 17 Slightly hard 1.0 to 3.5 17 to 60 Moderately hard 3.5 to 7.0 60 to 120 Hard 7.0 to 10.5 120 to 180 Very hard greater than 10.5 greater than 180
[0248] European water hardness is typically greater than about 10.5 (for example about 10.5 to about 20.0) grains per gallon mixed Ca2+/Mg2+ (e.g., about 15 grains per gallon mixed Ca2+/Mg2+). North American water hardness is typically greater than Japanese water hardness, but less than European water hardness. For example, North American water hardness can be between about 3 to about 10 grains, about 3 to about 8 grains or about 6 grains. Japanese water hardness is typically lower than North American water hardness, usually less than about 4, for example about 3 grains per gallon mixed Ca2+/Mg2+.
[0249] Accordingly, in some embodiments, the present invention provides metalloprotease polypeptides that show surprising wash performance in at least one set of wash conditions (e.g., water temperature, water hardness, and/or detergent concentration). In some embodiments, the metalloprotease polypeptides of the present invention are comparable in wash performance to other metalloprotease polypeptide proteases. In some embodiments of the present invention, the metalloprotease polypeptides provided herein exhibit enhanced oxidative stability, enhanced thermal stability, enhanced cleaning capabilities under various conditions, and/or enhanced chelator stability. In addition, the metalloprotease polypeptides of the present invention find use in cleaning compositions that do not include detergents, again either alone or in combination with builders and stabilizers.
[0250] In some embodiments of the present invention, the cleaning compositions comprise at least one metalloprotease polypeptide of the present invention at a level from about 0.00001% to about 10% by weight of the composition and the balance (e.g., about 99.999% to about 90.0%) comprising cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention comprises at least one metalloprotease polypeptide at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% by weight of the composition and the balance of the cleaning composition (e.g., about 99.9999% to about 90.0%, about 99.999% to about 98%, about 99.995% to about 99.5% by weight) comprising cleaning adjunct materials.
[0251] In some embodiments, the cleaning compositions of the present invention comprise one or more additional detergent enzymes, which provide cleaning performance and/or fabric care and/or dishwashing benefits. Examples of suitable enzymes include, but are not limited to, acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, or any combinations or mixtures thereof. In some embodiments, a combination of enzymes is used (i.e., a "cocktail") comprising conventional applicable enzymes like protease, lipase, cutinase and/or cellulase in conjunction with amylase is used.
[0252] In addition to the metalloprotease polypeptides provided herein, any other suitable protease finds use in the compositions of the present invention. Suitable proteases include those of animal, vegetable or microbial origin. In some embodiments, microbial proteases are used. In some embodiments, chemically or genetically modified mutants are included. In some embodiments, the protease is a serine protease, preferably an alkaline microbial protease or a trypsin-like protease. Examples of alkaline proteases include subtilisins, especially those derived from Bacillus (e.g., subtilisin, lentus, amyloliquefaciens, subtilisin Carlsberg, subtilisin 309, subtilisin 147 and subtilisin 168). Additional examples include those mutant proteases described in U.S. Pat. Nos. RE 34,606, 5,955,340, 5,700,676, 6,312,936, and 6,482,628, all of which are incorporated herein by reference. Additional protease examples include, but are not limited to trypsin (e.g., of porcine or bovine origin), and the Fusarium protease described in WO 89/06270. In some embodiments, commercially available protease enzymes that find use in the present invention include, but are not limited to MAXATASE®, MAXACAL®, MAXAPEM®, OPTICLEAN®, OPTIMASE®, PROPERASE®, PURAFECT®, PURAFECT® OXP, PURAMAX®, EXCELLASE®, and PURAFAST® (Genencor); ALCALASE®, SAVINASE®, PRIMASE®, DURAZYM®, POLARZYME®, OVOZYME®, KANNASE®, LIQUANASE®, NEUTRASE®, RELASE® and ESPERASE® (Novozymes); BLAP® and BLAP® variants (Henkel Kommanditgesellschaft auf Aktien, Duesseldorf, Germany), and KAP (B. alkalophilus subtilisin; Kao Corp., Tokyo, Japan). Various proteases are described in WO95/23221, WO 92/21760, WO 09/149200, WO 09/149144, WO 09/149145, WO 11/072099, WO 10/056640, WO 10/056653, WO 11/140364, WO 12/151534, U.S. Pat. Publ. No. 2008/0090747, and U.S. Pat. Nos. 5,801,039, 5,340,735, 5,500,364, 5,855,625, US RE 34,606, 5,955,340, 5,700,676, 6,312,936, and 6,482,628, and various other patents. In some further embodiments, metalloproteases find use in the present invention, including but not limited to the neutral metalloprotease described in WO 07/044993.
[0253] In addition, any suitable lipase finds use in the present invention. Suitable lipases include, but are not limited to those of bacterial or fungal origin. Chemically or genetically modified mutants are encompassed by the present invention. Examples of useful lipases include Humicola lanuginosa lipase (See e.g., EP 258 068, and EP 305 216), Rhizomucor miehei lipase (See e.g., EP 238 023), Candida lipase, such as C. antarctica lipase (e.g., the C. antarctica lipase A or B; See e.g., EP 214 761), Pseudomonas lipases such as P. alcaligenes lipase and P. pseudoalcaligenes lipase (See e.g., EP 218 272), P. cepacia lipase (See e.g., EP 331 376), P. stutzeri lipase (See e.g., GB 1,372,034), P. fluorescens lipase, Bacillus lipase (e.g., B. subtilis lipase [Dartois et al., Biochem. Biophys. Acta 1131:253-260
[1993]); B. stearothermophilus lipase [See e.g., JP 64/744992]; and B. pumilus lipase [See e.g., WO 91/16422]).
[0254] Furthermore, a number of cloned lipases find use in some embodiments of the present invention, including but not limited to Penicillium camembertii lipase (See, Yamaguchi et al., Gene 103:61-67
[1991]), Geotricum candidum lipase (See, Schimada et al., J. Biochem., 106:383-388
[1989]), and various Rhizopus lipases such as R. delemar lipase (See, Hass et al., Gene 109:117-113
[1991]), a R. niveus lipase (Kugimiya et al., Biosci. Biotech. Biochem. 56:716-719
[1992]) and R. oryzae lipase.
[0255] Other types of lipase polypeptide enzymes such as cutinases also find use in some embodiments of the present invention, including but not limited to the cutinase derived from Pseudomonas mendocina (See, WO 88/09367), and the cutinase derived from Fusarium solani pisi (See, WO 90/09446).
[0256] Additional suitable lipases include commercially available lipases such as M1 LIPASE®, LUMA FAST®, and LIPOMAX® (Genencor); LIPEX®, LIPOLASE® and LIPOLASE® ULTRA (Novozymes); and LIPASE P® "Amano" (Amano Pharmaceutical Co. Ltd., Japan).
[0257] In some embodiments of the present invention, the cleaning compositions of the present invention further comprise lipases at a level from about 0.00001% to about 10% of additional lipase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention also comprise lipases at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% lipase by weight of the composition.
[0258] In some embodiments of the present invention, any suitable amylase finds use in the present invention. In some embodiments, any amylase (e.g., alpha and/or beta) suitable for use in alkaline solutions also find use. Suitable amylases include, but are not limited to those of bacterial or fungal origin. Chemically or genetically modified mutants are included in some embodiments. Amylases that find use in the present invention, include, but are not limited to α-amylases obtained from B. licheniformis (See e.g., GB 1,296,839). Additional suitable amylases include those found in WO9510603, WO9526397, WO9623874, WO9623873, WO9741213, WO9919467, WO0060060, WO0029560, WO9923211, WO9946399, WO0060058, WO0060059, WO9942567, WO0114532, WO02092797, WO0166712, WO0188107, WO0196537, WO0210355, WO9402597, WO0231124, WO9943793, WO9943794, WO2004113551, WO2005001064, WO2005003311, WO0164852, WO2006063594, WO2006066594, WO2006066596, WO2006012899, WO2008092919, WO2008000825, WO2005018336, WO2005066338, WO2009140504, WO2005019443, WO2010091221, WO2010088447, WO0134784, WO2006012902, WO2006031554, WO2006136161, WO2008101894, WO2010059413, WO2011098531, WO2011080352, WO2011080353, WO2011080354, WO2011082425, WO2011082429, WO2011076123, WO2011087836, WO2011076897, WO94183314, WO9535382, WO9909183, WO9826078, WO9902702, WO9743424, WO9929876, WO9100353, WO9605295, WO9630481, WO9710342, WO2008088493, WO2009149419, WO2009061381, WO2009100102, WO2010104675, WO2010117511, and WO2010115021. Commercially available amylases that find use in the present invention include, but are not limited to DURAMYL®, TERMAMYL®, FUNGAMYL®, STAINZYME®, STAINZYME PLUS®, STAINZYME ULTRA®, and BAN® (Novozymes), as well as POWERASE®, RAPIDASE® and MAXAMYL® P (Genencor).
[0259] In some embodiments of the present invention, the cleaning compositions of the present invention further comprise amylases at a level from about 0.00001% to about 10% of additional amylase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention also comprise amylases at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% amylase by weight of the composition.
[0260] In some further embodiments, any suitable cellulase finds used in the cleaning compositions of the present invention. Suitable cellulases include, but are not limited to those of bacterial or fungal origin. Chemically or genetically modified mutants are included in some embodiments. Suitable cellulases include, but are not limited to Humicola insolens cellulases (See e.g., U.S. Pat. No. 4,435,307). Especially suitable cellulases are the cellulases having color care benefits (See e.g., EP 0 495 257). Commercially available cellulases that find use in the present include, but are not limited to CELLUZYME, CELLUCLEAN, CAREZYME (Novozymes), PURADEX AND REVITALENZ (Danisco US Inc.), and KAC-500(B) (Kao Corporation). In some embodiments, cellulases are incorporated as portions or fragments of mature wild-type or variant cellulases, wherein a portion of the N-terminus is deleted (See e.g., U.S. Pat. No. 5,874,276). Additional suitable cellulases include those found in WO2005054475, WO2005056787, U.S. Pat. Nos. 7,449,318, and 7,833,773. In some embodiments, the cleaning compositions of the present invention further comprise cellulases at a level from about 0.00001% to about 10% of additional cellulase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention also comprise cellulases at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% cellulase by weight of the composition.
[0261] Any mannanase suitable for use in detergent compositions also finds use in the present invention. Suitable mannanases include, but are not limited to those of bacterial or fungal origin. Chemically or genetically modified mutants are included in some embodiments. Various mannanases are known which find use in the present invention (See e.g., U.S. Pat. Nos. 6,566,114; 6,602,842; 5,476,775 and 6,440,991, and U.S. Prov. App. Ser. No. 61/739,267; all of which are incorporated herein by reference). Commercially available mannanases that find use in the present invention include, but are not limited to MANNASTAR, PURABRITE, and MANNAWAY. In some embodiments, the cleaning compositions of the present invention further comprise mannanases at a level from about 0.00001% to about 10% of additional mannanase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some embodiments of the present invention, the cleaning compositions of the present invention also comprise mannanases at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% mannanase by weight of the composition.
[0262] In some embodiments, peroxidases are used in combination with hydrogen peroxide or a source thereof (e.g., a percarbonate, perborate or persulfate) in the compositions of the present invention. In some alternative embodiments, oxidases are used in combination with oxygen. Both types of enzymes are used for "solution bleaching" (i.e., to prevent transfer of a textile dye from a dyed fabric to another fabric when the fabrics are washed together in a wash liquor), preferably together with an enhancing agent (See e.g., WO 94/12621 and WO 95/01426). Suitable peroxidases/oxidases include, but are not limited to those of plant, bacterial or fungal origin. Chemically or genetically modified mutants are included in some embodiments. In some embodiments, the cleaning compositions of the present invention further comprise peroxidase and/or oxidase enzymes at a level from about 0.00001% to about 10% of additional peroxidase and/or oxidase by weight of the composition and the balance of cleaning adjunct materials by weight of composition. In some other embodiments of the present invention, the cleaning compositions of the present invention also comprise, peroxidase and/or oxidase enzymes at a level of about 0.0001% to about 10%, about 0.001% to about 5%, about 0.001% to about 2%, about 0.005% to about 0.5% peroxidase and/or oxidase enzymes by weight of the composition.
[0263] In some embodiments, additional enzymes find use, including but not limited to perhydrolases (See e.g., WO 05/056782). In addition, in some embodiments, mixtures of the above mentioned enzymes are encompassed herein, in particular one or more additional protease, amylase, lipase, mannanase, and/or at least one cellulase. Indeed, it is contemplated that various mixtures of these enzymes will find use in the present invention. It is also contemplated that the varying levels of the metalloprotease polypeptide (s) and one or more additional enzymes may both independently range to about 10%, the balance of the cleaning composition being cleaning adjunct materials. The specific selection of cleaning adjunct materials are readily made by considering the surface, item, or fabric to be cleaned, and the desired form of the composition for the cleaning conditions during use (e.g., through the wash detergent use).
[0264] Examples of suitable cleaning adjunct materials include, but are not limited to, surfactants, builders, bleaches, bleach activators, bleach catalysts, other enzymes, enzyme stabilizing systems, chelants, optical brighteners, soil release polymers, dye transfer agents, dye transfer inhibiting agents, catalytic materials, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal agents, structure elasticizing agents, dispersants, suds suppressors, dyes, perfumes, colorants, filler salts, hydrotropes, photoactivators, fluorescers, fabric conditioners, fabric softeners, carriers, hydrotropes, processing aids, solvents, pigments, hydrolyzable surfactants, preservatives, anti-oxidants, anti-shrinkage agents, anti-wrinkle agents, germicides, fungicides, color speckles, silvercare, anti-tarnish and/or anti-corrosion agents, alkalinity sources, solubilizing agents, carriers, processing aids, pigments, and pH control agents (See e.g., U.S. Pat. Nos. 6,610,642; 6,605,458; 5,705,464; 5,710,115; 5,698,504; 5,695,679; 5,686,014 and 5,646,101, all of which are incorporated herein by reference). Embodiments of specific cleaning composition materials are exemplified in detail below. In embodiments in which the cleaning adjunct materials are not compatible with the metalloprotease polypeptides of the present invention in the cleaning compositions, then suitable methods of keeping the cleaning adjunct materials and the protease(s) separated (i.e., not in contact with each other) until combination of the two components is appropriate are used. Such separation methods include any suitable method known in the art (e.g., gelcaps, encapsulation, tablets, physical separation, etc.).
[0265] In some embodiments, an effective amount of one or more metalloprotease polypeptide (s) provided herein is included in compositions useful for cleaning a variety of surfaces in need of proteinaceous stain removal. Such cleaning compositions include cleaning compositions for such applications as cleaning hard surfaces, fabrics, and dishes. Indeed, in some embodiments, the present invention provides fabric cleaning compositions, while in other embodiments, the present invention provides non-fabric cleaning compositions. Notably, the present invention also provides cleaning compositions suitable for personal care, including oral care (including dentrifices, toothpastes, mouthwashes, etc., as well as denture cleaning compositions), skin, and hair cleaning compositions. It is intended that the present invention encompass detergent compositions in any form (i.e., liquid, granular, bar, semi-solid, gels, emulsions, tablets, capsules, etc.).
[0266] By way of example, several cleaning compositions wherein the metalloprotease polypeptides of the present invention find use are described in greater detail below. In some embodiments in which the cleaning compositions of the present invention are formulated as compositions suitable for use in laundry machine washing method(s), the compositions of the present invention preferably contain at least one surfactant and at least one builder compound, as well as one or more cleaning adjunct materials preferably selected from organic polymeric compounds, bleaching agents, additional enzymes, suds suppressors, dispersants, lime-soap dispersants, soil suspension and anti-redeposition agents and corrosion inhibitors. In some embodiments, laundry compositions also contain softening agents (i.e., as additional cleaning adjunct materials). The compositions of the present invention also find use in detergent additive products in solid or liquid form. Such additive products are intended to supplement and/or boost the performance of conventional detergent compositions and can be added at any stage of the cleaning process. In some embodiments, the density of the laundry detergent compositions herein ranges from about 400 to about 1200 g/liter, while in other embodiments, it ranges from about 500 to about 950 g/liter of composition measured at 20° C.
[0267] In embodiments formulated as compositions for use in manual dishwashing methods, the compositions of the invention preferably contain at least one surfactant and preferably at least one additional cleaning adjunct material selected from organic polymeric compounds, suds enhancing agents, group II metal ions, solvents, hydrotropes and additional enzymes.
[0268] In some embodiments, various cleaning compositions such as those provided in U.S. Pat. No. 6,605,458, find use with the metalloprotease polypeptides of the present invention. Thus, in some embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention is a compact granular fabric cleaning composition, while in other embodiments, the composition is a granular fabric cleaning composition useful in the laundering of colored fabrics, in further embodiments, the composition is a granular fabric cleaning composition which provides softening through the wash capacity, in additional embodiments, the composition is a heavy duty liquid fabric cleaning composition. In some embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention are fabric cleaning compositions such as those described in U.S. Pat. Nos. 6,610,642 and 6,376,450. In addition, the metalloprotease polypeptides of the present invention find use in granular laundry detergent compositions of particular utility under European or Japanese washing conditions (See e.g., U.S. Pat. No. 6,610,642).
[0269] In some alternative embodiments, the present invention provides hard surface cleaning compositions comprising at least one metalloprotease polypeptide provided herein. Thus, in some embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention is a hard surface cleaning composition such as those described in U.S. Pat. Nos. 6,610,642; 6,376,450, and 6,376,450.
[0270] In yet further embodiments, the present invention provides dishwashing compositions comprising at least one metalloprotease polypeptide provided herein. Thus, in some embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention is a hard surface cleaning composition such as those in U.S. Pat. Nos. 6,610,642 and 6,376,450. In some still further embodiments, the present invention provides dishwashing compositions comprising at least one metalloprotease polypeptide provided herein. In some further embodiments, the compositions comprising at least one metalloprotease polypeptide of the present invention comprise oral care compositions such as those in U.S. Pat. Nos. 6,376,450, and 6,376,450. The formulations and descriptions of the compounds and cleaning adjunct materials contained in the aforementioned U.S. Pat. Nos. 6,376,450; 6,605,458; 6,605,458, and 6,610,642, find use with the metalloprotease polypeptides provided herein.
[0271] The cleaning compositions of the present invention are formulated into any suitable form and prepared by any process chosen by the formulator, non-limiting examples of which are described in U.S. Pat. Nos. 5,879,584; 5,691,297; 5,574,005; 5,569,645; 5,565,422; 5,516,448; 5,489,392, and 5,486,303, all of which are incorporated herein by reference. When a low pH cleaning composition is desired, the pH of such composition is adjusted via the addition of a material such as monoethanolamine or an acidic material such as HCl.
[0272] In some embodiments, the cleaning compositions of the present invention can be formulated to have an alkaline pH under wash conditions, such as a pH of from about 8.0 to about 12.0, or from about 8.5 to about 11.0, or from about 9.0 to about 11.0. In some embodiments, the cleaning compositions of the present invention can be formulated to have a neutral pH under wash conditions, such as a pH of from about 5.0 to about 8.0, or from about 5.5 to about 8.0, or from about 6.0 to about 8.0, or from about 6.0 to about 7.5. In some embodiments, the neutral pH conditions can be measured when the cleaning composition is dissolved 1:100 (wt:wt) in de-ionised water at 20° C., measured using a conventional pH meter.
[0273] While not essential for the purposes of the present invention, the non-limiting list of adjuncts illustrated hereinafter are suitable for use in the instant cleaning compositions. In some embodiments, these adjuncts are incorporated for example, to assist or enhance cleaning performance, for treatment of the substrate to be cleaned, or to modify the aesthetics of the cleaning composition as is the case with perfumes, colorants, dyes or the like. It is understood that such adjuncts are in addition to the metalloprotease polypeptides of the present invention. The precise nature of these additional components, and levels of incorporation thereof, will depend on the physical form of the composition and the nature of the cleaning operation for which it is to be used. Suitable adjunct materials include, but are not limited to, surfactants, builders, chelating agents, dye transfer inhibiting agents, deposition aids, dispersants, additional enzymes, and enzyme stabilizers, catalytic materials, bleach activators, bleach boosters, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydrotropes, processing aids and/or pigments. In addition to the disclosure below, suitable examples of such other adjuncts and levels of use are found in U.S. Pat. Nos. 5,576,282, 6,306,812, and 6,326,348, incorporated by reference. The aforementioned adjunct ingredients may constitute the balance of the cleaning compositions of the present invention.
[0274] In some embodiments, the cleaning compositions according to the present invention comprise an acidifying particle or an amino carboxylic builder. Examples of an amino carboxylic builder include aminocarboxylic acids, salts and derivatives thereof. In some embodiment, the amino carboxylic builder is an aminopolycarboxylic builder, such as glycine-N,N-diacetic acid or derivative of general formula MOOC--CHR--N(CH2COOM)2 where R is C1-12 alkyl and M is alkali metal. In some embodiments, the amino carboxylic builder can be methylglycine diacetic acid (MGDA), GLDA (glutamic-N,N-diacetic acid), iminodisuccinic acid (IDS), carboxymethyl inulin and salts and derivatives thereof, aspartic acid-N-monoacetic acid (ASMA), aspartic acid-N,N-diacetic acid (ASDA), aspartic acid-N-monopropionic acid (ASMP), iminodisuccinic acid (IDA), N-(2-sulfomethyl)aspartic acid (SMAS), N-(2-sulfoethyl)aspartic acid (SEAS), N-(2-sulfomethyl)glutamic acid (SMGL), N-(2-sulfoethyl)glutamic acid (SEGL), IDS (iminodiacetic acid) and salts and derivatives thereof such as N-methyliminodiacetic acid (MIDA), alpha-alanine-N,N-diacetic acid (alpha-ALDA), serine-N,N-diacetic acid (SEDA), isoserine-N,Ndiacetic acid (ISDA), phenylalanine-N,N-diacetic acid (PHDA), anthranilic acid-N,N-diacetic acid (ANDA), sulfanilic acid-N,N-diacetic acid (SLDA), taurine-N,N-diacetic acid (TUDA) and sulfomethyl-N,N-diacetic acid (SMDA) and alkali metal salts and derivative thereof. In some embodiments, the acidifying particle has a weight geometric mean particle size of from about 400μ to about 1200μ and a bulk density of at least 550 g/L. In some embodiments, the acidifying particle comprises at least about 5% of the builder.
[0275] In some embodiments, the acidifying particle can comprise any acid, including organic acids and mineral acids. Organic acids can have one or two carboxyls and in some instances up to 15 carbons, especially up to 10 carbons, such as formic, acetic, propionic, capric, oxalic, succinic, adipic, maleic, fumaric, sebacic, malic, lactic, glycolic, tartaric and glyoxylic acids. In some embodiments, the acid is citric acid. Mineral acids include hydrochloric and sulphuric acid. In some instances, the acidifying particle of the invention is a highly active particle comprising a high level of amino carboxylic builder. Sulphuric acid has been found to further contribute to the stability of the final particle.
[0276] In some embodiments, the cleaning compositions according to the present invention comprise at least one surfactant and/or a surfactant system wherein the surfactant is selected from nonionic surfactants, anionic surfactants, cationic surfactants, ampholytic surfactants, zwitterionic surfactants, semi-polar nonionic surfactants and mixtures thereof. In some low pH cleaning composition embodiments (e.g., compositions having a neat pH of from about 3 to about 5), the composition typically does not contain alkyl ethoxylated sulfate, as it is believed that such surfactant may be hydrolyzed by such compositions the acidic contents. In some embodiments, the surfactant is present at a level of from about 0.1% to about 60%, while in alternative embodiments the level is from about 1% to about 50%, while in still further embodiments the level is from about 5% to about 40%, by weight of the cleaning composition.
[0277] In some embodiments, the cleaning compositions of the present invention comprise one or more detergent builders or builder systems. In some embodiments incorporating at least one builder, the cleaning compositions comprise at least about 1%, from about 3% to about 60% or even from about 5% to about 40% builder by weight of the cleaning composition. Builders include, but are not limited to, the alkali metal, ammonium and alkanolammonium salts of polyphosphates, alkali metal silicates, alkaline earth and alkali metal carbonates, aluminosilicates, polycarboxylate compounds, ether hydroxypolycarboxylates, copolymers of maleic anhydride with ethylene or vinyl methyl ether, 1,3,5-trihydroxy benzene-2,4,6-trisulphonic acid, and carboxymethyloxysuccinic acid, the various alkali metal, ammonium and substituted ammonium salts of polyacetic acids such as ethylenediamine tetraacetic acid and nitrilotriacetic acid, as well as polycarboxylates such as mellitic acid, succinic acid, citric acid, oxydisuccinic acid, polymaleic acid, benzene 1,3,5-tricarboxylic acid, carboxymethyloxysuccinic acid, and soluble salts thereof. Indeed, it is contemplated that any suitable builder will find use in various embodiments of the present invention.
[0278] In some embodiments, the builders form water-soluble hardness ion complexes (e.g., sequestering builders), such as citrates and polyphosphates (e.g., sodium tripolyphosphate and sodium tripolyphospate hexahydrate, potassium tripolyphosphate, and mixed sodium and potassium tripolyphosphate, etc.). It is contemplated that any suitable builder will find use in the present invention, including those known in the art (See e.g., EP 2 100 949).
[0279] In some embodiments, builders for use herein include phosphate builders and non-phosphate builders. In some embodiments, the builder is a phosphate builder. In some embodiments, the builder is a non-phosphate builder. If present, builders are used in a level of from 0.1% to 80%, or from 5 to 60%, or from 10 to 50% by weight of the composition. In some embodiments the product comprises a mixture of phosphate and non-phosphate builders. Suitable phosphate builders include mono-phosphates, di-phosphates, tri-polyphosphates or oligomeric-poylphosphates, including the alkali metal salts of these compounds, including the sodium salts. In some embodiments, a builder can be sodium tripolyphosphate (STPP). Additionally, the composition can comprise carbonate and/or citrate, preferably citrate that helps to achieve a neutral pH composition of the invention. Other suitable non-phosphate builders include homopolymers and copolymers of polycarboxylic acids and their partially or completely neutralized salts, monomeric polycarboxylic acids and hydroxycarboxylic acids and their salts. In some embodiments, salts of the above mentioned compounds include the ammonium and/or alkali metal salts, i.e. the lithium, sodium, and potassium salts, including sodium salts. Suitable polycarboxylic acids include acyclic, alicyclic, hetero-cyclic and aromatic carboxylic acids, wherein in some embodiments, they can contain at least two carboxyl groups which are in each case separated from one another by, in some instances, no more than two carbon atoms.
[0280] In some embodiments, the cleaning compositions of the present invention contain at least one chelating agent. Suitable chelating agents include, but are not limited to copper, iron and/or manganese chelating agents and mixtures thereof. In embodiments in which at least one chelating agent is used, the cleaning compositions of the present invention comprise from about 0.1% to about 15% or even from about 3.0% to about 10% chelating agent by weight of the subject cleaning composition.
[0281] In some still further embodiments, the cleaning compositions provided herein contain at least one deposition aid. Suitable deposition aids include, but are not limited to, polyethylene glycol, polypropylene glycol, polycarboxylate, soil release polymers such as polytelephthalic acid, clays such as kaolinite, montmorillonite, atapulgite, illite, bentonite, halloysite, and mixtures thereof.
[0282] As indicated herein, in some embodiments, anti-redeposition agents find use in some embodiments of the present invention. In some embodiments, non-ionic surfactants find use. For example, in automatic dishwashing embodiments, non-ionic surfactants find use for surface modification purposes, in particular for sheeting, to avoid filming and spotting and to improve shine. These non-ionic surfactants also find use in preventing the re-deposition of soils. In some embodiments, the anti-redeposition agent is a non-ionic surfactant as known in the art (See e.g., EP 2 100 949). In some embodiments, the non-ionic surfactant can be ethoxylated nonionic surfactants, epoxy-capped poly(oxyalkylated) alcohols and amine oxides surfactants.
[0283] In some embodiments, the cleaning compositions of the present invention include one or more dye transfer inhibiting agents. Suitable polymeric dye transfer inhibiting agents include, but are not limited to, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidones and polyvinylimidazoles or mixtures thereof. In embodiments in which at least one dye transfer inhibiting agent is used, the cleaning compositions of the present invention comprise from about 0.0001% to about 10%, from about 0.01% to about 5%, or even from about 0.1% to about 3% by weight of the cleaning composition.
[0284] In some embodiments, silicates are included within the compositions of the present invention. In some such embodiments, sodium silicates (e.g., sodium disilicate, sodium metasilicate, and crystalline phyllosilicates) find use. In some embodiments, silicates are present at a level of from about 1% to about 20%. In some embodiments, silicates are present at a level of from about 5% to about 15% by weight of the composition.
[0285] In some still additional embodiments, the cleaning compositions of the present invention also contain dispersants. Suitable water-soluble organic materials include, but are not limited to the homo- or co-polymeric acids or their salts, in which the polycarboxylic acid comprises at least two carboxyl radicals separated from each other by not more than two carbon atoms.
[0286] In some further embodiments, the enzymes used in the cleaning compositions are stabilized by any suitable technique. In some embodiments, the enzymes employed herein are stabilized by the presence of water-soluble sources of calcium and/or magnesium ions in the finished compositions that provide such ions to the enzymes. In some embodiments, the enzyme stabilizers include oligosaccharides, polysaccharides, and inorganic divalent metal salts, including alkaline earth metals, such as calcium salts, such as calcium formate. It is contemplated that various techniques for enzyme stabilization will find use in the present invention. For example, in some embodiments, the enzymes employed herein are stabilized by the presence of water-soluble sources of zinc (II), calcium (II) and/or magnesium (II) ions in the finished compositions that provide such ions to the enzymes, as well as other metal ions (e.g., barium (II), scandium (II), iron (II), manganese (II), aluminum (III), Tin (II), cobalt (II), copper (II), nickel (II), and oxovanadium (IV). Chlorides and sulfates also find use in some embodiments of the present invention. Examples of suitable oligosaccharides and polysaccharides (e.g., dextrins) are known in the art (See e.g., WO 07/145964). In some embodiments, reversible protease inhibitors also find use, such as boron-containing compounds (e.g., borate, 4-formyl phenyl boronic acid) and/or a tripeptide aldehyde find use to further improve stability, as desired.
[0287] In some embodiments, bleaches, bleach activators and/or bleach catalysts are present in the compositions of the present invention. In some embodiments, the cleaning compositions of the present invention comprise inorganic and/or organic bleaching compound(s). Inorganic bleaches include, but are not limited to perhydrate salts (e.g., perborate, percarbonate, perphosphate, persulfate, and persilicate salts). In some embodiments, inorganic perhydrate salts are alkali metal salts. In some embodiments, inorganic perhydrate salts are included as the crystalline solid, without additional protection, although in some other embodiments, the salt is coated. Any suitable salt known in the art finds use in the present invention (See e.g., EP 2 100 949).
[0288] In some embodiments, bleach activators are used in the compositions of the present invention. Bleach activators are typically organic peracid precursors that enhance the bleaching action in the course of cleaning at temperatures of 60° C. and below. Bleach activators suitable for use herein include compounds which, under perhydrolysis conditions, give aliphatic peroxoycarboxylic acids having preferably from about 1 to about 10 carbon atoms, in particular from about 2 to about 4 carbon atoms, and/or optionally substituted perbenzoic acid. Additional bleach activators are known in the art and find use in the present invention (See e.g., EP 2 100 949).
[0289] In addition, in some embodiments and as further described herein, the cleaning compositions of the present invention further comprise at least one bleach catalyst. In some embodiments, the manganese triazacyclononane and related complexes find use, as well as cobalt, copper, manganese, and iron complexes. Additional bleach catalysts find use in the present invention (See e.g., U.S. Pat. Nos. 4,246,612, 5,227,084, 4,810410, WO 99/06521, and EP 2 100 949).
[0290] In some embodiments, the cleaning compositions of the present invention contain one or more catalytic metal complexes. In some embodiments, a metal-containing bleach catalyst finds use. In some embodiments, the metal bleach catalyst comprises a catalyst system comprising a transition metal cation of defined bleach catalytic activity, (e.g., copper, iron, titanium, ruthenium, tungsten, molybdenum, or manganese cations), an auxiliary metal cation having little or no bleach catalytic activity (e.g., zinc or aluminum cations), and a sequestrate having defined stability constants for the catalytic and auxiliary metal cations, particularly ethylenediaminetetraacetic acid, ethylenediaminetetra (methylenephosphonic acid) and water-soluble salts thereof are used (See e.g., U.S. Pat. No. 4,430,243). In some embodiments, the cleaning compositions of the present invention are catalyzed by means of a manganese compound. Such compounds and levels of use are well known in the art (See e.g., U.S. Pat. No. 5,576,282). In additional embodiments, cobalt bleach catalysts find use in the cleaning compositions of the present invention. Various cobalt bleach catalysts are known in the art (See e.g., U.S. Pat. Nos. 5,597,936 and 5,595,967) and are readily prepared by known procedures.
[0291] In some additional embodiments, the cleaning compositions of the present invention include a transition metal complex of a macropolycyclic rigid ligand (MRL). As a practical matter, and not by way of limitation, in some embodiments, the compositions and cleaning processes provided by the present invention are adjusted to provide on the order of at least one part per hundred million of the active MRL species in the aqueous washing medium, and in some embodiments, provide from about 0.005 ppm to about 25 ppm, more preferably from about 0.05 ppm to about 10 ppm, and most preferably from about 0.1 ppm to about 5 ppm, of the MRL in the wash liquor.
[0292] In some embodiments, transition-metals in the instant transition-metal bleach catalyst include, but are not limited to manganese, iron and chromium. MRLs also include, but are not limited to special ultra-rigid ligands that are cross-bridged (e.g., 5,12-diethyl-1,5,8,12-tetraazabicyclo[6.6.2]hexadecane). Suitable transition metal MRLs are readily prepared by known procedures (See e.g., WO 2000/32601, and U.S. Pat. No. 6,225,464).
[0293] In some embodiments, the cleaning compositions of the present invention comprise metal care agents. Metal care agents find use in preventing and/or reducing the tarnishing, corrosion, and/or oxidation of metals, including aluminum, stainless steel, and non-ferrous metals (e.g., silver and copper). Suitable metal care agents include those described in EP 2 100 949, WO 9426860 and WO 94/26859). In some embodiments, the metal care agent is a zinc salt. In some further embodiments, the cleaning compositions of the present invention comprise from about 0.1% to about 5% by weight of one or more metal care agent.
[0294] In some embodiments, the cleaning composition is a high density liquid (HDL) composition having a variant metalloprotease polypeptide protease. The HDL liquid laundry detergent can comprise a detersive surfactant (10%-40%) comprising anionic detersive surfactant (selected from a group of linear or branched or random chain, substituted or unsubstituted alkyl sulphates, alkyl sulphonates, alkyl alkoxylated sulphate, alkyl phosphates, alkyl phosphonates, alkyl carboxylates, and/or mixtures thereof); and optionally non-ionic surfactant (selected from a group of linear or branched or random chain, substituted or unsubstituted alkyl alkoxylated alcohol, for example a C8-C18 alkyl ethoxylated alcohol and/or C6-C12 alkyl phenol alkoxylates), optionally wherein the weight ratio of anionic detersive surfactant (with a hydrophilic index (HIc) of from 6.0 to 9) to non-ionic detersive surfactant is greater than 1:1.
[0295] The composition can comprise optionally, a surfactancy boosting polymer consisting of amphiphilic alkoxylated grease cleaning polymers (selected from a group of alkoxylated polymers having branched hydrophilic and hydrophobic properties, such as alkoxylated polyalkylenimines in the range of 0.05 wt %-10 wt %) and/or random graft polymers (typically comprising of hydrophilic backbone comprising monomers selected from the group consisting of: unsaturated C1-C6 carboxylic acids, ethers, alcohols, aldehydes, ketones, esters, sugar units, alkoxy units, maleic anhydride, saturated polyalcohols such as glycerol, and mixtures thereof; and hydrophobic side chain(s) selected from the group consisting of: C4-C25 alkyl group, polypropylene, polybutylene, vinyl ester of a saturated C--C6 mono-carboxylic acid, C1-C6 alkyl ester of acrylic or methacrylic acid, and mixtures thereof.
[0296] The composition can comprise additional polymers such as soil release polymers (include anionically end-capped polyesters, for example SRP1, polymers comprising at least one monomer unit selected from saccharide, dicarboxylic acid, polyol and combinations thereof, in random or block configuration, ethylene terephthalate-based polymers and co-polymers thereof in random or block configuration, for example Repel-o-tex SF, SF-2 and SRP6, Texcare SRA100, SRA300, SRN100, SRN170, SRN240, SRN300 and SRN325, Marloquest SL), anti-redeposition polymers (0.1 wt % to 10 wt %, include carboxylate polymers, such as polymers comprising at least one monomer selected from acrylic acid, maleic acid (or maleic anhydride), fumaric acid, itaconic acid, aconitic acid, mesaconic acid, citraconic acid, methylenemalonic acid, and any mixture thereof, vinylpyrrolidone homopolymer, and/or polyethylene glycol, molecular weight in the range of from 500 to 100,000 Da); cellulosic polymer (including those selected from alkyl cellulose, alkyl alkoxyalkyl cellulose, carboxyalkyl cellulose, alkyl carboxyalkyl cellulose examples of which include carboxymethyl cellulose, methyl cellulose, methyl hydroxyethyl cellulose, methyl carboxymethyl cellulose, and mixtures thereof) and polymeric carboxylate (such as maleate/acrylate random copolymer or polyacrylate homopolymer).
[0297] The composition can further comprise saturated or unsaturated fatty acid, preferably saturated or unsaturated C12-C24 fatty acid (0 wt % to 10 wt %); deposition aids (examples for which include polysaccharides, preferably cellulosic polymers, poly diallyl dimethyl ammonium halides (DADMAC), and co-polymers of DAD MAC with vinyl pyrrolidone, acrylamides, imidazoles, imidazolinium halides, and mixtures thereof, in random or block configuration, cationic guar gum, cationic cellulose such as cationic hydoxyethyl cellulose, cationic starch, cationic polyacylamides, and mixtures thereof.
[0298] The composition can further comprise dye transfer inhibiting agents examples of which include manganese phthalocyanine, peroxidases, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidones and polyvinylimidazoles and/or mixtures thereof; chelating agents examples of which include ethylene-diamine-tetraacetic acid (EDTA); diethylene triamine penta methylene phosphonic acid (DTPMP); hydroxy-ethane diphosphonic acid (HEDP); ethylenediamine N,N'-disuccinic acid (EDDS); methyl glycine diacetic acid (MGDA); diethylene triamine penta acetic acid (DTPA); propylene diamine tetracetic acid (PDT A); 2-hydroxypyridine-N-oxide (HPNO); or methyl glycine diacetic acid (MGDA); glutamic acid N,N-diacetic acid (N,N-dicarboxymethyl glutamic acid tetrasodium salt (GLDA); nitrilotriacetic acid (NTA); 4,5-dihydroxy-m-benzenedisulfonic acid; citric acid and any salts thereof; N-hydroxyethylethylenediaminetri-acetic acid (HEDTA), triethylenetetraaminehexaacetic acid (TTHA), N-hydroxyethyliminodiacetic acid (HEIDA), dihydroxyethylglycine (DHEG), ethylenediaminetetrapropionic acid (EDTP) and derivatives thereof.
[0299] The composition can further comprise enzymes (0.01 wt % active enzyme to 0.03 wt % active enzyme) selected from a group of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, and any mixture thereof. The composition may comprise an enzyme stabilizer (examples of which include polyols such as propylene glycol or glycerol, sugar or sugar alcohol, lactic acid, reversible protease inhibitor, boric acid, a boric acid derivative, e.g., an aromatic borate ester, or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid, peptides or formate).
[0300] The composition can further comprise silicone or fatty-acid based suds suppressors; heuing dyes, calcium and magnesium cations, visual signaling ingredients, anti-foam (0.001 wt % to about 4.0 wt %), and/or structurant/thickener (0.01 wt % to 5 wt %, selected from the group consisting of diglycerides and triglycerides, ethylene glycol distearate, microcrystalline cellulose, cellulose based materials, microfiber cellulose, biopolymers, xanthan gum, gellan gum, and mixtures thereof).
[0301] Suitable detersive surfactants also include cationic detersive surfactants (selected from a group of alkyl pyridinium compounds, alkyl quarternary ammonium compounds, alkyl quarternary phosphonium compounds, alkyl ternary sulphonium compounds, and/or mixtures thereof); zwitterionic and/or amphoteric detersive surfactants (selected from a group of alkanolamine sulpho-betaines); ampholytic surfactants; semi-polar non-ionic surfactants and mixtures thereof.
[0302] The composition can be any liquid form, for example a liquid or gel form, or any combination thereof. The composition may be in any unit dose form, for example a pouch.
[0303] In some embodiments, the cleaning composition is a high density powder (HDD) composition having a variant metalloprotease polypeptide protease. The HDD powder laundry detergent can comprise a detersive surfactant including anionic detersive surfactants (selected from a group of linear or branched or random chain, substituted or unsubstituted alkyl sulphates, alkyl sulphonates, alkyl alkoxylated sulphate, alkyl phosphates, alkyl phosphonates, alkyl carboxylates and/or mixtures thereof), non-ionic detersive surfactant (selected from a group of linear or branched or random chain, substituted or unsubstituted C8-C18 alkyl ethoxylates, and/or C6-C12 alkyl phenol alkoxylates), cationic detersive surfactants (selected from a group of alkyl pyridinium compounds, alkyl quaternary ammonium compounds, alkyl quaternary phosphonium compounds, alkyl ternary sulphonium compounds, and mixtures thereof), zwitterionic and/or amphoteric detersive surfactants (selected from a group of alkanolamine sulpho-betaines); ampholytic surfactants; semi-polar non-ionic surfactants and mixtures thereof; builders (phosphate free builders [for example zeolite builders examples of which include zeolite A, zeolite X, zeolite P and zeolite MAP in the range of 0 wt % to less than 10 wt %]; phosphate builders [examples of which include sodium tri-polyphosphate in the range of 0 wt % to less than 10 wt %]; citric acid, citrate salts and nitrilotriacetic acid or salt thereof in the range of less than 15 wt %); silicate salt (sodium or potassium silicate or sodium meta-silicate in the range of 0 wt % to less than 10 wt %, or layered silicate (SKS-6)); carbonate salt (sodium carbonate and/or sodium bicarbonate in the range of 0 wt % to less than 10 wt %); and bleaching agents (photobleaches, examples of which include sulfonated zinc phthalocyanines, sulfonated aluminum phthalocyanines, xanthenes dyes, and mixtures thereof; hydrophobic or hydrophilic bleach activators (examples of which include dodecanoyl oxybenzene sulfonate, decanoyl oxybenzene sulfonate, decanoyl oxybenzoic acid or salts thereof, 3,5,5-trimethy hexanoyl oxybenzene sulfonate, tetraacetyl ethylene diamine-TAED, and nonanoyloxybenzene sulfonate-NOBS, nitrile quats, and mixtures thereof; hydrogen peroxide; sources of hydrogen peroxide (inorganic perhydrate salts examples of which include mono or tetra hydrate sodium salt of perborate, percarbonate, persulfate, perphosphate, or persilicate); preformed hydrophilic and/or hydrophobic peracids (selected from a group consisting of percarboxylic acids and salts, percarbonic acids and salts, perimidic acids and salts, peroxymonosulfuric acids and salts) & mixtures thereof and/or bleach catalyst (such as imine bleach boosters examples of which include iminium cations and polyions; iminium zwitterions; modified amines; modified amine oxides; N-sulphonyl imines; N-phosphonyl imines; N-acyl imines; thiadiazole dioxides; perfluoroimines; cyclic sugar ketones and mixtures thereof; metal-containing bleach catalyst for example copper, iron, titanium, ruthenium, tungsten, molybdenum, or manganese cations along with an auxiliary metal cations such as zinc or aluminum and a sequestrate such as ethylenediaminetetraacetic acid, ethylenediaminetetra(methylenephos-phonic acid) and water-soluble salts thereof).
[0304] The composition can further comprise enzymes selected from a group of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases and any mixture thereof.
[0305] The composition can further comprise additional detergent ingredients including perfume microcapsules, starch encapsulated perfume accord, hueing agents, additional polymers including fabric integrity and cationic polymers, dye lock ingredients, fabric-softening agents, brighteners (for example C.I. Fluorescent brighteners), flocculating agents, chelating agents, alkoxylated polyamines, fabric deposition aids, and/or cyclodextrin.
[0306] In some embodiments, the cleaning composition is an automatic dishwashing (ADW) detergent composition having a metalloprotease of the present invention. The ADW detergent composition can comprise two or more non-ionic surfactants selected from a group of ethoxylated non-ionic surfactants, alcohol alkoxylated surfactants, epoxy-capped poly(oxyalkylated) alcohols, or amine oxide surfactants present in amounts from 0 to 10% by weight; builders in the range of 5-60% comprising either phosphate (mono-phosphates, di-phosphates, tri-polyphosphates or oligomeric-poylphosphates, preferred sodium tripolyphosphate-STPP or phosphate-free builders [amino acid based compounds, examples of which include MGDA (methyl-glycine-diacetic acid), and salts and derivatives thereof, GLDA (glutamic-N,Ndiacetic acid) and salts and derivatives thereof, IDS (iminodisuccinic acid) and salts and derivatives thereof, carboxy methyl inulin and salts and derivatives thereof and mixtures thereof, nitrilotriacetic acid (NTA), diethylene triamine penta acetic acid (DTPA), B-alaninediacetic acid (B-ADA) and their salts], homopolymers and copolymers of poly-carboxylic acids and their partially or completely neutralized salts, monomeric polycarboxylic acids and hydroxycarboxylic acids and their salts in the range of 0.5% to 50% by weight; sulfonated/carboxylated polymers (provide dimensional stability to the product) in the range of about 0.1% to about 50% by weight; drying aids in the range of about 0.1% to about 10% by weight (selected from polyesters, especially anionic polyesters optionally together with further monomers with 3 to 6 functionalities which are conducive to polycondensation, specifically acid, alcohol or ester functionalities, polycarbonate-, polyurethane- and/or polyurea-polyorganosiloxane compounds or precursor compounds thereof of the reactive cyclic carbonate and urea type); silicates in the range from about 1% to about 20% by weight (sodium or potassium silicates for example sodium disilicate, sodium meta-silicate and crystalline phyllosilicates); bleach-inorganic (for example perhydrate salts such as perborate, percarbonate, perphosphate, persulfate and persilicate salts) and organic (for example organic peroxyacids including diacyl and tetraacylperoxides, especially diperoxydodecanedioc acid, diperoxytetradecanedioc acid, and diperoxyhexadecanedioc acid); bleach activators-organic peracid precursors in the range from about 0.1% to about 10% by weight; bleach catalysts (selected from manganese triazacyclononane and related complexes, Co, Cu, Mn and Fe bispyridylamine and related complexes, and pentamine acetate cobalt(III) and related complexes); metal care agents in the range from about 0.1% to 5% by weight (selected from benzatriazoles, metal salts and complexes, and/or silicates); enzymes in the range from about 0.01 to 5.0 mg of active enzyme per gram of automatic dishwashing detergent composition (acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, and any mixture thereof); and enzyme stabilizer components (selected from oligosaccharides, polysaccharides and inorganic divalent metal salts).
[0307] The metalloproteases are normally incorporated into the detergent composition at a level of from 0.000001% to 5% of enzyme protein by weight of the composition, or from 0.00001% to 2%, or from 0.0001% to 1%, or from 0.001% to 0.75% of enzyme protein by weight of the composition.
Metalloprotease Polypeptides of the Present Invention for Use in Animal Feed
[0308] In a further aspect of the invention, the metalloprotease polypeptides of the present invention can be used as a component of an animal feed composition, animal feed additive and/or pet food comprising a metalloprotease and variants thereof. The present invention further relates to a method for preparing such an animal feed composition, animal feed additive composition and/or pet food comprising mixing the metalloprotease polypeptide with one or more animal feed ingredients and/or animal feed additive ingredients and/or pet food ingredients. Furthermore, the present invention relates to the use of the metalloprotease polypeptide in the preparation of an animal feed composition and/or animal feed additive composition and/or pet food.
[0309] The term "animal" includes all non-ruminant and ruminant animals. In a particular embodiment, the animal is a non-ruminant animal, such as a horse and a mono-gastric animal. Examples of mono-gastric animals include, but are not limited to, pigs and swine, such as piglets, growing pigs, sows; poultry such as turkeys, ducks, chicken, broiler chicks, layers; fish such as salmon, trout, tilapia, catfish and carps; and crustaceans such as shrimps and prawns. In a further embodiment the animal is a ruminant animal including, but not limited to, cattle, young calves, goats, sheep, giraffes, bison, moose, elk, yaks, water buffalo, deer, camels, alpacas, llamas, antelope, pronghorn and nilgai.
[0310] In the present context, it is intended that the term "pet food" is understood to mean a food for a household animal such as, but not limited to, dogs, cats, gerbils, hamsters, chinchillas, fancy rats, guinea pigs; avian pets, such as canaries, parakeets, and parrots; reptile pets, such as turtles, lizards and snakes; and aquatic pets, such as tropical fish and frogs.
[0311] The terms "animal feed composition," "feedstuff" and "fodder" are used interchangeably and can comprise one or more feed materials selected from the group comprising a) cereals, such as small grains (e.g., wheat, barley, rye, oats and combinations thereof) and/or large grains such as maize or sorghum; b) by products from cereals, such as corn gluten meal, Distillers Dried Grain Solubles (DDGS) (particularly corn based Distillers Dried Grain Solubles (cDDGS), wheat bran, wheat middlings, wheat shorts, rice bran, rice hulls, oat hulls, palm kernel, and citrus pulp; c) protein obtained from sources such as soya, sunflower, peanut, lupin, peas, fava beans, cotton, canola, fish meal, dried plasma protein, meat and bone meal, potato protein, whey, copra, sesame; d) oils and fats obtained from vegetable and animal sources; e) minerals and vitamins.
Metalloprotease Polypeptides of the Present Invention for Use in Textile Desizing
[0312] Also contemplated are compositions and methods of treating fabrics (e.g., to desize a textile) using a metalloprotease polypeptide of the present invention. Fabric-treating methods are well known in the art (see, e.g., U.S. Pat. No. 6,077,316). For example, the feel and appearance of a fabric can be improved by a method comprising contacting the fabric with a metalloprotease in a solution. The fabric can be treated with the solution under pressure.
[0313] A metalloprotease of the present invention can be applied during or after the weaving of a textile, or during the desizing stage, or one or more additional fabric processing steps. During the weaving of textiles, the threads are exposed to considerable mechanical strain. Prior to weaving on mechanical looms, warp yarns are often coated with sizing starch or starch derivatives to increase their tensile strength and to prevent breaking. A metalloprotease of the present invention can be applied during or after the weaving to remove these sizing starch or starch derivatives. After weaving, the metalloprotease can be used to remove the size coating before further processing the fabric to ensure a homogeneous and wash-proof result.
[0314] A metalloprotease of the present invention can be used alone or with other desizing chemical reagents and/or desizing enzymes to desize fabrics, including cotton-containing fabrics, as detergent additives, e.g., in aqueous compositions. An amylase also can be used in compositions and methods for producing a stonewashed look on indigo-dyed denim fabric and garments. For the manufacture of clothes, the fabric can be cut and sewn into clothes or garments, which are afterwards finished. In particular, for the manufacture of denim jeans, different enzymatic finishing methods have been developed. The finishing of denim garment normally is initiated with an enzymatic desizing step, during which garments are subjected to the action of proteolytic enzymes to provide softness to the fabric and make the cotton more accessible to the subsequent enzymatic finishing steps. The metalloprotease can be used in methods of finishing denim garments (e.g., a "bio-stoning process"), enzymatic desizing and providing softness to fabrics, and/or finishing process.
Metalloprotease Polypeptides of the Present Invention for Use in Paper Pulp Bleaching
[0315] The metalloprotease polypeptides described herein find further use in the enzyme aided bleaching of paper pulps such as chemical pulps, semi-chemical pulps, kraft pulps, mechanical pulps or pulps prepared by the sulfite method. In general terms, paper pulps are incubated with a metalloprotease polypeptide of the present invention under conditions suitable for bleaching the paper pulp.
[0316] In some embodiments, the pulps are chlorine free pulps bleached with oxygen, ozone, peroxide or peroxyacids. In some embodiments, the metalloprotease polypeptides are used in enzyme aided bleaching of pulps produced by modified or continuous pulping methods that exhibit low lignin contents. In some other embodiments, the metalloprotease polypeptides are applied alone or preferably in combination with xylanase and/or endoglucanase and/or alpha-galactosidase and/or cellobiohydrolase enzymes.
Metalloprotease Polypeptides of the Present Invention for Use in Protein Degradation
[0317] The metalloprotease polypeptides described herein find further use in the enzyme aided removal of proteins from animals and their subsequent degradation or disposal, such as feathers, skin, hair, hide, and the like. In some instances, immersion of the animal carcass in a solution comprising a metalloprotease polypeptide of the present invention can act to protect the skin from damage in comparison to the traditional immersion in scalding water or the defeathering process. In one embodiment, feathers can be sprayed with an isolated metalloprotase polypeptide of the present invention under conditions suitable for digesting or initiating degradation of the plumage. In some embodiments, a metalloprotease of the present invention can be used, as above, in combination with an oxidizing agent.
[0318] In some embodiments, removal of the oil or fat associated with raw feathers is assisted by using a metalloprotease polypeptide of the present invention. In some embodiments, the metalloprotease polypeptides are used in compositions for cleaning the feathers as well as to sanitize and partially dehydrate the fibers. In some other embodiments, the metalloprotease polypeptides are applied in a wash solution in combination with 95% ethanol or other polar organic solvent with or without a surfactant at about 0.5% (v/v).
[0319] In yet other embodiments, the disclosed metalloprotease polypeptides find use in recovering protein from plumage. The disclosed metalloprotease polypeptides may be used alone or in combination in suitable feather processing and proteolytic methods, such as those disclosed in PCT/EP2013/065362, PCT/EP2013/065363, and PCT/EP2013/065364, which are hereby incorporated by reference. In some embodiments, the recovered protein can be subsequently used in animal or fish feed.
EXPERIMENTAL
[0320] The claimed invention is described in further detail in the following examples which are not in any way intended to limit the scope of the invention as claimed.
Example 1.1
Cloning of Paenibacillus sp. Metalloprotease PspPro3
[0321] A strain of Paenibacillus sp. was selected as a potential source for enzymes which may be useful for various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hours. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the Paenibacillus sp. strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina's next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in Paenibacillus sp. encodes a metalloprotease and the sequence of this gene, called PspPro3, is provided in SEQ ID NO: 1. The corresponding protein encoded by the PspPro3 gene is shown in SEQ ID NO: 2. At the N-terminus, the protein has a signal peptide with a length of 26 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PspPro3 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the Paenibacillus polymyxa Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PspPro3 protein is shown on SEQ ID NO: 3.
[0322] The nucleotide sequence of the PspPro3 gene isolated from Paenibacillus sp. is set forth as SEQ ID NO: 1. The sequence encoding the predicted native signal peptide is shown in italics:
TABLE-US-00002 ATGTTAATGAAAAAAGTATGGGTTTCGCTTCTTGGAGGAGCGATGTTATT AGGGTCTGTAGCGTCTGGTGCATCAGCAGCGGAGAGTTCCGTTTCGGGGC CGGCTCAGCTTACGCCAACCTTCCATGCCGAACAATGGAAAGCACCTTCA TCGGTATCGGGTGATGACATCGTATGGAGCTATTTAAATCGGCAAAAGAA AACGTTGCTGGGTACGGACAGCACCAGTGTCCGTGATCAATTCCGTATCG TAGATCGCACAAGCGACAAATCCGGCGTGAGCCATTATCGGCTGAAGCAA TATGTAAACGGAATTCCCGTATATGGAGCTGAACAGACCATTCATGTGGG CAAATCCGGTGAAGTGACCTCTTATCTGGGAGCCGTGATTACTGAGGATC AGCAAGAAGAAGCTACGCAAGGTACAACTCCGAAAATCAGCGCTTCTGAA GCGGTCCATACCGCATATCAGGAGGCAGCTACACGGGTTCAAGCCCTCCC TACCTCCGATGATACGATTTCTAAAGATGCGGAGGAGCCAAGCAGTGTAA GCAAAGACACTTACTCCGAAGCAGCTAACAACGGAAAAACGAGTTCTGTT GAAAAGGACAAGCTCAGCCTTGAGAAAGCGGCTGACCTGAAAGATAGCAA AATTGAAGCGGTGGAGGCAGAGCCAAACTCCATTGCCAAAATCGCCAACC TGCAGCCTGAGGTAGATCCTAAAGCCGAACTATATTTCTATGCGAAGGGC GATGCATTGCAGCTGGTTTATGTGACTGAGGTTAATATTTTGCAGCCTGC GCCGCTGCGTACACGCTACATCATTGACGCCAATGATGGCAAAATCGTAT CCCAGTATGACATCATTAATGAAGCGACAGGCACAGGCAAAGGTGTACTC GGTGATACCAAAACATTCAACACTACTGCTTCCGGCAGCAGCTACCAGTT AAGAGATACGACTCGCGGGAATGGAATCGTGACTTACACGGCCTCCAACC GTCAAAGCATCCCAGGTACGATCCTGACCGATGCCGATAACGTATGGAAT GATCCAGCCGGCGTGGATGCCCACGCTTATGCAGCCAAAACCTATGATTA TTATAAGGAAAAGTTCAATCGCAACAGCATTGACGGACGAGGCCTGCAGC TCCGTTCGACAGTTCATTACGGCAATCGTTACAACAACGCCTTCTGGAAC GGCTCCCAAATGACTTATGGAGACGGAGACGGCACCACATTTATCGCTTT TAGCGGTGATCCGGATGTAGTTGGTCATGAACTCACACACGGTGTTACGG AGTATACTTCCAATTTGGAATATTACGGAGAATCCGGTGCGTTGAACGAG GCCTTCTCGGACATCATCGGCAATGACATCCAGCGTAAAAACTGGCTTGT AGGCGATGATATTTACACGCCACGCATTGCGGGTGATGCACTTCGTTCTA TGTCCAATCCTACGCTGTACGATCAACCGGATCACTATTCGAACTTGTAC AGAGGCAGCTCCGATAACGGCGGCGTTCATACGAACAGCGGTATTATAAA TAAAGCCTATTATCTGTTGGCACAAGGCGGCACCTTCCATGGTGTAACTG TCAATGGGATTGGCCGCGATGCAGCGGTTCAAATTTACTACAGCGCCTTT ACGAACTACCTGACTTCTTCTTCTGACTTCTCCAATGCACGTGATGCCGT TGTACAAGCGGCAAAAGATCTCTACGGCGCGAGCTCGGCACAAGCTACCG CAGCAGCCAAATCTTTTGATGCTGTAGGCGTTAAC
[0323] The amino acid sequence of the PspPro3 precursor protein is set forth as SEQ ID NO: 2. The predicted signal peptide is shown in italics, and the predicted pro-peptide is shown in underlined text:
TABLE-US-00003 MLMKKVWVSLLGGAMLLGSVASGASAAESSVSGPAQLTPTFHAEQWKAPS SVSGDDIVWSYLNRQKKTLLGTDSTSVRDQFRIVDRTSDKSGVSHYRLKQ YVNGIPVYGAEQTIHVGKSGEVTSYLGAVITEDQQEEATQGTTPKISASE AVHTAYQEAATRVQALPTSDDTISKDAEEPSSVSKDTYSEAANNGKTSSV EKDKLSLEKAADLKDSKIEAVEAEPNSIAKIANLQPEVDPKAELYFYAKG DALQLVYVTEVNILQPAPLRTRYIIDANDGKIVSQYDIINEATGTGKGVL GDTKTFNTTASGSSYQLRDTTRGNGIVTYTASNRQSIPGTILTDADNVWN DPAGVDAHAYAAKTYDYYKEKFNRNSIDGRGLQLRSTVHYGNRYNNAFWN GSQMTYGDGDGTTFIAFSGDPDVVGHELTHGVTEYTSNLEYYGESGALNE AFSDIIGNDIQRKNWLVGDDIYTPRIAGDALRSMSNPTLYDQPDHYSNLY RGSSDNGGVHTNSGIINKAYYLLAQGGTFHGVTVNGIGRDAAVQIYYSAF TNYLTSSSDFSNARDAVVQAAKDLYGASSAQATAAAKSFDAVGVN
[0324] The amino acid sequence of the predicted mature form of PspPro3 is set forth as SEQ ID NO: 3:
TABLE-US-00004 ATGTGKGVLGDTKTFNTTASGSSYQLRDTTRGNGIVTYTASNRQSIPGTI LTDADNVWNDPAGVDAHAYAAKTYDYYKEKFNRNSIDGRGLQLRSTVHYG NRYNNAFWNGSQMTYGDGDGTTFIAFSGDPDVVGHELTHGVTEYTSNLEY YGESGALNEAFSDIIGNDIQRKNWLVGDDIYTPRIAGDALRSMSNPTLYD QPDHYSNLYRGSSDNGGVHTNSGIINKAYYLLAQGGTFHGVTVNGIGRDA AVQIYYSAFTNYLTSSSDFSNARDAVVQAAKDLYGASSAQATAAAKSFDA VGVN
Example 1.2
Expression of Paenibacillus sp. Metalloprotease PspPro3
[0325] The DNA sequence of the propeptide-mature form of PspPro3 was synthesized and inserted into the Bacillus subtilis expression vector p2JM103BBI (Vogtentanz, Protein Expr Purif, 55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX085 (AprE-PspPro3) (FIG. 1.1). Ligation of the gene encoding the PspPro3 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3' end of the Bacillus subtilis AprE signal sequence and the 5' end of the predicted PspPro3 native propeptide. The gene has an alternative start codon (GTG). As shown in FIG. 1.1, pGX085(AprE-PspPro3) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in B. subtilis, and the synthetic nucleotide sequence encoding the predicted propeptide and mature region of PspPro3 (SEQ ID NO: 4). The translation product of the synthetic AprE-PspPro3 gene is shown in SEQ ID NO: 5.
[0326] B. subtilis cells (degU.sup.Hy32, ΔscoC) were transformed with the pGX085(AprE-PspPro3) plasmid and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat#232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl2). The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl2 and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to a 150 mL Q Sepharose High Performance column pre-equilibrated with the loading buffer above and PspPro3 was then eluted from the column via the loading buffer supplemented with a linear NaCl gradient from 0 to 0.7 M. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses.
[0327] The nucleotide sequence of the synthesized PspPro3 gene in plasmid pGX085(AprE-PspPro3) is depicted in SEQ ID NO: 4. The sequence encoding the predicted native signal peptide is shown in italics and the region encoding the three residue addition (AGK) is shown in bold:
TABLE-US-00005 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAAT CTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCTGCTGGAAAAGCAG AATCATCAGTGTCAGGACCGGCTCAGCTTACGCCGACGTTTCATGCAGAG CAGTGGAAAGCACCGAGCAGCGTTAGCGGAGATGACATCGTGTGGAGCTA CCTGAACAGACAGAAGAAAACGCTTCTTGGCACGGACAGCACGAGCGTCA GAGACCAGTTCAGAATCGTGGATAGAACAAGCGACAAAAGCGGCGTCAGC CATTATAGACTGAAGCAGTATGTGAACGGAATCCCGGTTTATGGCGCAGA ACAAACAATCCATGTCGGAAAGAGCGGCGAAGTTACGAGCTATCTGGGCG CGGTTATTACAGAGGACCAGCAAGAGGAGGCTACACAAGGCACGACACCG AAAATTTCAGCATCAGAGGCAGTTCATACGGCCTACCAAGAAGCTGCAAC GAGAGTTCAAGCCCTGCCTACGTCAGATGATACAATCAGCAAAGACGCTG AGGAACCTAGCTCAGTTAGCAAGGACACGTATAGCGAAGCCGCGAACAAT GGCAAGACGTCAAGCGTGGAAAAAGACAAGCTTTCACTGGAGAAGGCCGC TGATCTGAAAGACTCAAAGATCGAGGCTGTGGAAGCGGAACCGAATAGCA TTGCAAAGATTGCCAACCTGCAACCGGAGGTGGACCCGAAGGCGGAGCTG TATTTCTACGCTAAAGGCGATGCACTGCAACTGGTTTACGTCACGGAGGT TAACATCCTGCAGCCGGCACCGCTTAGAACGAGATACATCATTGACGCGA ACGACGGCAAGATCGTGAGCCAGTACGACATTATCAACGAGGCCACGGGA ACGGGCAAGGGAGTCCTTGGCGACACGAAGACATTCAATACAACGGCCTC AGGCTCATCATACCAGCTGAGAGACACGACGAGAGGCAACGGAATCGTCA CGTACACGGCTAGCAATAGACAGAGCATTCCGGGCACAATCCTTACGGAC GCAGACAATGTGTGGAATGACCCGGCAGGCGTGGACGCACATGCCTACGC AGCGAAGACGTACGACTACTACAAGGAGAAGTTCAACAGAAACAGCATCG ACGGAAGAGGACTGCAACTTAGAAGCACGGTGCATTACGGCAACAGATAC AACAACGCTTTCTGGAACGGCAGCCAAATGACGTATGGAGACGGCGATGG AACAACGTTTATCGCATTCTCAGGCGACCCTGACGTTGTGGGACATGAAC TGACGCATGGAGTCACAGAATACACGAGCAATCTGGAGTATTACGGAGAA TCAGGCGCACTTAATGAGGCCTTCAGCGACATCATCGGAAACGACATCCA GAGAAAGAACTGGCTGGTTGGCGATGATATCTACACGCCGAGAATTGCGG GCGACGCGCTGAGATCAATGAGCAACCCTACGCTGTACGATCAGCCGGAT CATTACAGCAACCTGTATAGAGGCTCAAGCGATAATGGCGGCGTGCATAC AAACAGCGGCATCATCAACAAAGCCTATTATCTGCTGGCGCAAGGCGGCA CATTCCATGGCGTTACAGTTAATGGCATTGGCAGAGACGCAGCCGTGCAG ATCTACTACAGCGCATTCACGAATTACCTGACATCAAGCAGCGACTTTTC AAATGCAAGAGATGCAGTGGTGCAGGCGGCTAAAGACCTTTATGGAGCTT CAAGCGCTCAGGCCACAGCTGCGGCAAAAAGCTTCGACGCGGTTGGAGTG AAT
[0328] The amino acid sequence of the PspPro3 precursor protein expressed from plasmid pGX085(AprE-PspPro3) is depicted in SEQ ID NO: 5. The predicted signal sequence is shown in italics, the three residue addition (AGK) shown in bold and the predicted pro-peptide is shown in underlined text.:
TABLE-US-00006 MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKAESSVSGPAQLTPTFHAE QWKAPSSVSGDDIVWSYLNRQKKTLLGTDSTSVRDQFRIVDRTSDKSGVS HYRLKQYVNGIPVYGAEQTIHVGKSGEVTSYLGAVITEDQQEEATQGTTP KISASEAVHTAYQEAATRVQALPTSDDTISKDAEEPSSVSKDTYSEAANN GKTSSVEKDKLSLEKAADLKDSKIEAVEAEPNSIAKIANLQPEVDPKAEL YFYAKGDALQLVYVTEVNILQPAPLRTRYIIDANDGKIVSQYDIINEATG TGKGVLGDTKTFNTTASGSSYQLRDTTRGNGIVTYTASNRQSIPGTILTD ADNVWNDPAGVDAHAYAAKTYDYYKEKFNRNSIDGRGLQLRSTVHYGNRY NNAFWNGSQMTYGDGDGTTFIAFSGDPDVVGHELTHGVTEYTSNLEYYGE SGALNEAFSDIIGNDIQRKNWLVGDDIYTPRIAGDALRSMSNPTLYDQPD HYSNLYRGSSDNGGVHTNSGIINKAYYLLAQGGTFHGVTVNGIGRDAAVQ IYYSAFTNYLTSSSDFSNARDAVVQAAKDLYGASSAQATAAAKSFDAV GVN
[0329] The amino acid sequence of the PspPro3 recombinant protein isolated from Bacillus subtilis culture was determined by tandem mass spectrometry, and shown below. It is the same as predicted and depicted in SEQ ID NO: 3.
TABLE-US-00007 ATGTGKGVLGDTKTFNTTASGSSYQLRDTTRGNGIVTYTASNRQSIPGTI LTDADNVWNDPAGVDAHAYAAKTYDYYKEKFNRNSIDGRGLQLRSTVHYG NRYNNAFWNGSQMTYGDGDGTTFIAFSGDPDVVGHELTHGVTEYTSNLEY YGESGALNEAFSDIIGNDIQRKNWLVGDDIYTPRIAGDALRSMSNPTLYD QPDHYSNLYRGSSDNGGVHTNSGIINKAYYLLAQGGTFHGVTVNGIGRDA AVQIYYSAFTNYLTSSSDFSNARDAVVQAAKDLYGASSAQATAAAKSFDA VGVN
Example 1.3
Proteolytic Activity of Metalloprotease PspPro3
[0330] The proteolytic activity of purified PspPro3 was measured in 50 mM Tris (pH 7), using azo-casein (Cat#74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μL of the diluted enzyme (or Milli-Q H2O alone as the blank control) was added to the non-binding 96-well microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μL of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μL of 5% Trichloroacetic Acid (TCA). Following equilibration (5 mM at the room temperature) and subsequent centrifugation (2000 g for 10 mM at 4° C.), 120 μL supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A440) using a SpectraMax 190. Net A440 was calculated by subtracting the A440 of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of duplicate assays, and the value varies no more than 5%. The proteolytic activity is shown as Net A440. The proteolytic assay with azo-casein as the substrate (FIG. 1.2) indicates that PspPro3 is an active protease.
Example 1.4
pH Profile of Metalloprotease PspPro3
[0331] With azo-casein as the substrate, the pH profile of PspPro3 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μL of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μL diluted enzyme (250 ppm in Milli-Q H2O) in a 96-MTP placed on ice, followed by the addition of 48 μL of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 1.3. Enzyme activity at each pH was reported as relative activity where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in FIG. 1.3, the optimal pH of PspPro3 is 7.5, with greater than 70% of maximal activity retained between pH 5.5 and 9.
Example 1.5
Temperature Profile of Metalloprotease PspPro3
[0332] The temperature profiles of PspPro3 were analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assay. The enzyme sample and azo-casein substrate were prepared as in Example 3. Prior to the reaction, 50 μL of 1.5% azo-casein and 45 μl Milli-Q H2O were mixed in a 200 μL PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20˜90° C.) for 5 min. After the incubation, 5 μL of diluted PspPro3 (100 ppm) or H2O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μL of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 1.3. The activity was reported as relative activity where the activity at the optimal temperature was set to be 100%. The tested temperatures were 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of triplicate assays. The data in FIG. 1.4 suggest that PspPro3 showed an optimal temperature at 50° C., and retained greater than 70% of its maximal activity between 45'C and 60° C.
Example 1.6
Cleaning Performance of Metalloprotease PspPro3 in Automatic Dishwashing (ADW) Conditions
[0333] The cleaning performance of PspPro3 in automatic dishwashing (ADW) conditions was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 or 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified PspPro3 were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent (composition shown in Table 1.1) with 100 ppm water hardness (Ca2+:Mg2+=3:1), in the absence or presence of a bleach component (Peracid N,N-phthaloylaminoperoxycaproic acid-PAP). To initiate the reaction, 180 μL of AT detergent buffered at pH 6 or 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μL of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μL of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (A405) (referred here as the "Initial performance") using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μL water. Following the addition of 180 μL of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliter of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as "Wash-off"). The sum of two absorbance measurements ("Initial performance" plus "Wash-off") gives the "Total performance", which measures the protease activity on the model stain. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 for PspPro3 in AT detergent, in the absence or presence of bleach, is shown in FIGS. 5A and 5B, respectively.
TABLE-US-00008 TABLE 1.1 Composition of AT dish detergent formula with bleach Concentration Ingredient (mg/ml) MGDA (methylglycinediacetic acid) 0.143 Sodium citrate 1.86 Citric acid* varies PAP (peracid N,N-phthaloylaminoperoxycaproic acid) 0.057 Plurafac ® LF 18B (a non-ionic surfactant) 0.029 Bismuthcitrate 0.006 Bayhibit ® S (Phosphonobutantricarboxylic acid 0.006 sodium salt) Acusol ® 587 (a calcium polyphosphate inhibitor) 0.029 PEG 6000 0.043 PEG 1500 0.1 *The pH of the AT detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid.
Example 1.7
Cleaning Performance of Metalloprotease PspPro3 in Laundry Conditions
[0334] The cleaning performance of PspPro3 protein in liquid laundry detergent was tested using EMPA-116 (cotton soiled with blood/milk/ink) microswatches (obtained from CFT Vlaardingen, The Netherlands) at pH 8.2 using a commercial detergent. Prior to the reaction, purified PspPro3 protein samples were diluted with a dilution solution (10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol) to the desired concentrations; and the commercial detergent (Tide®, Clean Breeze®, Proctor & Gamble, USA, purchased September 2011) was incubated at 95° C. for 1 hour to inactivate the enzymes present in the detergent. Proteolytic assays were subsequently performed to confirm the inactivation of proteases in the commercial detergent. The heat treated detergent was further diluted with 5 mM HEPES (pH 8.2) to a final concentration of 0.788 g/L. Meanwhile, the water hardness of the buffered liquid detergent was adjusted to 103 ppm (Ca2+:Mg2+=3:1). The specific conductivity of the buffered detergent was adjusted to either 0.62 mS/cm (low conductivity) or 3.5 mS/cm (high conductivity) by adjusting the NaCl concentration in the buffered detergent. To initiate the reaction, 190 μl of either the high or low conductivity buffered detergent was added to a 96-MTP containing the EMPA-116 microswatches, followed by the addition of 10 μl of diluted enzyme (or the dilution solution as blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 20 min at 32° C. and 1150 rpm. After incubation, 150 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 600 nm using a spectrophotometer, which indicates the protease activity on the model stain; and Net A600 was subsequently calculated by subtracting the A600 of the blank control from that of the enzyme. Dose response for the cleaning of EMPA-116 microswatches in liquid laundry detergent at high or low conductivity is shown in FIG. 1.6.
Example 1.8
Comparison of PspPro3 to Other Metalloproteases
[0335] A. Identification of Homologous Proteases
[0336] Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The mature protein amino acid sequence for PspPro3 (SEQ ID NO: 3) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 1.2A and 1.2B provide a list of sequences with the percent identity to PspPro3. The length in Table 1.2 refers to the entire sequence length of the homologous proteases.
TABLE-US-00009 TABLE 1.2A List of sequences with percent identity to PspPro3 protein identified from the NCBI non-redundant protein database PID to Accession # PspPro3 Organism Length ZP_10321515.1 55 Bacillus macauensis ZFHKF-1 552 AAC43402.1 57 Alicyclobacillus acidocaldarius 546 P00800 57 Bacillus thermoproteolyticus 548 AAA22621.1 58 Geobacillus stearothermophilus 548 ZP_01862236.1 59 Bacillus sp. SG-1 560 YP_002884504.1 59 Exiguobacterium sp. AT1b 509 AEI46285.1 60 Paenibacillus mucilaginosus 525 KNP414 ZP_08093424 60 Planococcus donghaensis 553 MPA1U2 ZP_10324092.1 61 Bacillus macauensis ZFHKF-1 533 YP_006792441.1 61 Exiguobacterium antarcticum B7 498 AAK69076.1 63 Bacillus thuringiensis serovar 566 finitimus NP_976992.1 64 Bacillus cereus ATCC 10987 566 ZP_04321694 64 Bacillus cereus 566 BAA06144 64 Lactobacillus sp. 566 ZP_10241029.1 78 Paenibacillus peoriae KCTC 3763 599 YP_005073223 93 Paenibacillus terrae HPL-003 591 YP_003872179 94 Paenibacillus polymyxa E681 592 ZP_09775364 100 Paenibacillus sp. Aloe-11 593
TABLE-US-00010 TABLE 1.2B List of sequences with percent identity to PspPro3 protein identified from the Genome Quest Patent database PID to Patent # PspPro3 Organism Length US20120107907-0184 57.88 Bacillus caldoyticus 319 US20120107907-0177 57.88 Bacillus caldolyticus 544 WO2012110563-0002 58.2 Bacillus caldolyticus 319 EP2390321-0176 58.52 Bacillus stearothermophilis 548 US6518054-0002 59.22 Bacillus sp. 316 WO2004011619-0044 60.6 Empty 507 WO2004011619-0047 62.14 Empty 532 WO2004011619-0046 62.26 Empty 536 WO2012110563-0004 63.02 Bacillus megaterium 320 JP2002272453-0003 63.67 Empty 562 US8114656-0186 64.24 Bacillus brevis 304 WO2012110562-0005 64.52 Bacillus cereus 320 WO2007044993-0178 64.74 Bacillus thuringiensis 566 EP2178896-0184 65.38 Bacillus anthracis 566 WO2012110563-0005 65.48 Bacillus cereus 320 JP1995184649-0001 65.71 Lactobacillus sp. 566 US5962264-0004 65.81 Empty 566 US20120107907-0185 66.13 Bacillus cereus 317 US8114656-0187 93.36 Bacillus polymyxa 302 JP2005229807-0019 93.38 Paenibacillus polymyxa 566
[0337] B. Alignment of Homologous Protease Sequences
[0338] The amino acid sequence for mature PspPro3 (SEQ ID NO: 3) was aligned with thermolysin (P00800, Bacillus thermoproteolyticus) and protease from Paenibacillus sp. Aloe-11 (ZP_09775364) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters. FIG. 1.7 shows the alignment of PspPro3 with these protease sequences.
[0339] C. Phylogenetic Tree
[0340] A phylogenetic tree for full length sequence of PspPro3 (SEQ ID NO: 2) was built using sequences of representative homologs from Tables 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. MolBiol. Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in FIG. 1.8.
Example 1.9
Terg-o-Tometer Performance Evaluation of PspPro3
[0341] The wash performance of PspPro3 was tested in a laundry detergent application using a Terg-o-Tometer (Instrument Marketing Services, Inc, Fairfield, N.J.). The performance evaluation was conducted at 32° C. and 16° C. The soil load consisted of two of each of the following stain swatches: EMPA116 Blood, Milk, Ink on cotton (Test materials AG, St. Gallen, Switzerland), EMPA117 Blood, Milk, Ink on polycotton (Test materials AG, St. Gallen, Switzerland), EMPA112 Cocoa on cotton (Test materials AG, St. Gallen, Switzerland), and CFT C-10 Pigment, Oil, and Milk content on cotton (Center for Testmaterials BV, Vlaardingen, Netherlands), plus extra white interlock knit fabric to bring the total fabric load to 40 g per beaker of the Terg-o-Tometer, which was filled with 1 L of deionized water. The water hardness was adjusted to 6 grains per gallon, and the pH in the beaker was buffered with 5 mM HEPES, pH 8.2. Heat inactivated Tide Regular HDL (Procter & Gamble), a commercial liquid detergent purchased in a local US supermarket, was used at 0.8 g/L. The detergent was inactivated before use by treatment at 92° C. in a water bath for 2-3 hours followed by cooling to room temperature. Heat inactivation of commercial detergents serves to destroy the activity of enzymatic components while retaining the properties of the non-enzymatic components. Enzyme activity in the heat inactivated detergent was measured using the Suc-AAPF-pNA assay for measuring protease activity. The Purafect® Prime HA, (Genencor Int'l) and PspPro3 proteases were each added to final concentrations of 0, 0.2, 0.5, 1, and 1.5 ppm. The wash time was 12 minutes. After the wash treatment, all swatches were rinsed for 3 minutes and machine-dried at low heat.
[0342] Four of each types of swatch were measured before and after treatment by optical reflectance using a Tristimulus Minolta Meter CR-400. The difference in the L, a, b values was converted to total color difference (dE), as defined by the CIE-LAB color space. Cleaning of the stains is expressed as percent stain removal index (% SRI) by taking a ratio between the color difference before and after washing, and comparing it to the difference of unwashed soils (before wash) to unsoiled fabric, and averaging the eight values obtained by reading two different regions of each washed swatch and is reported in Tables 1.9A and 1.9B as Average % SRI (dE)±95CI. Table 1.9A summarizes the cleaning performance of PspPro3 at 32° C. and Table 1.9B at 16° C.
TABLE-US-00011 TABLE 1.9A Cleaning performance of PspPro3 at 32° C. Average 95CI Average 95CI Average 95CI Average 95CI ppm % SRI [% SRI % SRI [% SRI % SRI [% SRI % SRI [% SRI enzyme (dE) (dE)] (dE) (dE)] (dE) (dE)] (dE) (dE)] EMPA-116 EMPA-117 Purafect Prime Purafect Prime HA PspPro3 HA SprPro3 0 0.19 0.01 0.19 0.01 0.17 0.01 0.17 0.01 0.2 0.27 0.02 0.27 0.02 0.25 0.03 0.30 0.02 0.5 0.28 0.03 0.31 0.01 0.30 0.03 0.31 0.02 1 0.30 0.01 0.32 0.02 0.35 0.02 0.34 0.03 1.5 0.31 0.02 0.31 0.01 0.37 0.01 0.37 0.03 EMPA-112 CFT C-10 Purafect Prime Purafect Prime HA PspPro3 HA PspPro3 0 0.11 0.03 0.11 0.03 0.07 0.01 0.07 0.01 0.2 0.11 0.05 0.18 0.04 0.12 0.01 0.11 0.01 0.5 0.13 0.04 0.17 0.03 0.15 0.01 0.16 0.01 1 0.18 0.03 0.19 0.04 0.17 0.01 0.21 0.01 1.5 0.19 0.03 0.18 0.04 0.18 0.01 0.23 0.01
TABLE-US-00012 TABLE 1.9B Cleaning performance of PspPro3 at 16° C. Purafect Prime Purafect Prime HA PspPro3 HA PspPro3 Average 95CI Average 95CI Average 95CI Average 95CI ppm % SRI [% SRI % SRI [% SRI % SRI [% SRI % SRI [% SRI enzyme (dE) (dE)] (dE) (dE)] (dE) (dE)] (dE) (dE)] EMPA-116 EMPA-117 0 0.15 0.02 0.15 0.02 0.13 0.01 0.13 0.01 0.2 0.19 0.02 0.20 0.03 0.15 0.02 0.15 0.02 0.5 0.20 0.02 0.19 0.02 0.21 0.02 0.20 0.02 1 0.24 0.04 0.21 0.02 0.22 0.02 0.20 0.01 1.5 0.19 0.02 0.25 0.04 0.23 0.03 0.20 0.01 EMPA-112 CFT C-10 0 0.08 0.03 0.08 0.03 0.04 0.08 0.04 0.08 0.2 0.12 0.02 0.09 0.01 0.06 0.12 0.06 0.09 0.5 0.08 0.02 0.11 0.02 0.08 0.08 0.08 0.11 1 0.11 0.02 0.10 0.03 0.08 0.11 0.09 0.10 1.5 0.13 0.02 0.11 0.03 0.11 0.13 0.10 0.11
Example 2.1
Cloning of Metalloprotease PspPro2 from Paenibacillus sp.
[0343] A strain of Paenibacillus sp. was selected as a potential source for enzymes which may be useful for various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the Paenibacillus sp. strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina's next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in Paenibacillus sp. encodes a metalloprotease and the sequence of this gene, called PspPro2, is provided in SEQ ID NO: 6. The corresponding protein encoded by the PspPro2 gene is shown in SEQ ID NO: 7. At the N-terminus, the protein has a signal peptide with a length of 24 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PspPro2 is a secreted enzyme. The propeptide region of PspPro2 was predicted based on protein sequence alignment with the Paenibacillus polymyxa Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PspPro2 is shown in SEQ ID NO: 8.
[0344] The nucleotide sequence of the PspPro2 gene isolated from Paenibacillus sp. is set forth as SEQ ID NO: 6. The sequence encoding the predicted native signal peptide is shown in italics:
TABLE-US-00013 ATGAAAAAAGTATGGGTTTCACTTCTTGGAGGAGCGATGTTATTAGGGGC TGTAGCACCAGGTGCATCAGCAGCAGAGCATTCTGTTCCTGATCCTACTC AGCTAACACCGACCTTTCACGCCGAGCAATGGAAGGCTCCTTCCACGGTA ACCGGCGACAATATTGTATGGAGCTATTTGAATCGACAAAAGAAAACCTT ATTGAATACAGACAGCACCAGTGTGCGTGATCAGTTCCGCATCATTGATC GTACAAGCGACAAATCCGGTGCAAGCCATTATCGGCTCAAGCAATATGTA AACGGGATCCCCGTATATGGGGCTGAACAGACCATTCATGTGAACAACGC CGGTAAAGTAACCTCTTATTTGGGTGCTGTCATTTCAGAGGATCAGCAGC AAGACGCGACCGAAGATACCACTCCAAAAATCAGCGCGACTGAAGCCGTT TATACCGCATATGCAGAAGCCGCTGCCCGGATTCAATCCTTCCCTTCCAT CAATGATAGTCTTTCTGAGGCTAGTGAGGAACAAGGGAGTGAGAATCAAG GCAATGAGATTCAAAACATTGGGATTAAAAGCAGTGTAAGTAATGACACT TACGCAGAGGCGCATAACAACGTACTTTTAACCCCCGTTGACCAAGCAGA GCAAAGTTACATTGCCAAAATTGCTAATCTGGAGCCAAGTGTAGAGCCCA AAGCAGAATTATACATCTATCCAGATGGTGAGACTACACGACTGGTTTAT GTAACAGAGGTTAATATTCTTGAACCTGCGCCTCTGCGCACACGCTACTT CATTGATGCGAAAACCGGCAAAATCGTATTCCAGTATGACATCCTCAACC ACGCAACAGGCACCGGCCGCGGCGTGGATGGCAAAACAAAATCATTTACG ACTACAGCTTCAGGCAACCGGTATCAGTTGAAAGACACGACTCGCAGCAA TGGAATCGTGACTTACACCGCTGGCAATCGCCAGACGACGCCAGGTACGA TTTTGACCGATACAGATAATGTATGGGAGGACCCTGCGGCTGTTGATGCC CATGCCTACGCCATTAAAACCTATGACTATTATAAGAATAAATTCGGTCG CGACAGTATTGATGGACGTGGCATGCAAATTCGTTCGACAGTCCATTACG GCAAAAAATATAACAATGCCTTCTGGAACGGCTCGCAAATGACCTACGGA GACGGAGACGGGTCCACATTTACCTTCTTCAGCGGCGATCCCGATGTCGT GGGGCATGAGCTCACCCACGGCGTCACCGAGTTCACCTCCAATTTGGAGT ATTATGGTGAGTCCGGTGCATTGAACGAAGCCTTCTCGGATATTATCGGT AATGATATAGATGGCACCAGTTGGCTTCTTGGCGACGGCATTTATACGCC TAATATTCCAGGCGACGCTCTGCGTTCCCTGTCCGATCCTACACGATTCG GCCAGCCGGATCACTACTCCAATTTCTATCCGGACCCCAACAATGATGAT GAAGGCGGAGTCCATACGAACAGCGGTATTATCAACAAAGCCTATTATTT GCTGGCACAAGGCGGTACGTCCCATGGTGTAACGGTAACTGGTATCGGAC GCGAAGCGGCTGTATTCATTTACTACAATGCCTTTACCAACTATTTGACC TCTACCTCCAACTTCTCTAACGCACGCGCTGCTGTTATACAGGCAGCCAA GGATTTTTATGGTGCTGATTCGCTGGCAGTAACCAGTGCTATTCAATCCT TTGATGCGGTAGGAATCAAA
[0345] The amino acid sequence of the PspPro2 precursor protein is set forth as SEQ ID NO: 7. The predicted signal peptide is shown in italics, and the predicted pro-peptide is shown in underlined text:
TABLE-US-00014 MKKVWVSLLGGAMLLGAVAPGASAAEHSVPDPTQLTPTFHAEQWKAPSTV TGDNIVWSYLNRQKKTLLNTDSTSVRDQFRIIDRTSDKSGASHYRLKQYV NGIPVYGAEQTIHVNNAGKVTSYLGAVISEDQQQDATEDTTPKISATEAV YTAYAEAAARIQSFPSINDSLSEASEEQGSENQGNEIQNIGIKSSVSNDT YAEAHNNVLLTPVDQAEQSYIAKIANLEPSVEPKAELYIYPDGETTRLVY VTEVNILEPAPLRTRYFIDAKTGKIVFQYDILNHATGTGRGVDGKTKSFT TTASGNRYQLKDTTRSNGIVTYTAGNRQTTPGTILTDTDNVWEDPAAVDA HAYAIKTYDYYKNKFGRDSIDGRGMQIRSTVHYGKKYNNAFWNGSQMTYG DGDGSTFTFFSGDPDVVGHELTHGVTEFTSNLEYYGESGALNEAFSDIIG NDIDGTSWLLGDGIYTPNIPGDALRSLSDPTRFGQPDHYSNFYPDPNNDD EGGVHTNSGIINKAYYLLAQGGTSHGVTVTGIGREAAVFIYYNAFTNYLT STSNFSNARAAVIQAAKDFYGADSLAVTSAIQSFDAVGIK
[0346] The amino acid sequence of the predicted mature form of PspPro2 is set forth as SEQ ID NO: 8.
TABLE-US-00015 ATGTGRGVDGKTKSFTTTASGNRYQLKDTTRSNGIVTYTAGNRQTTPGTI LTDTDNVWEDPAAVDAHAYAIKTYDYYKNKFGRDSIDGRGMQIRSTVHYG KKYNNAFWNGSQMTYGDGDGSTFTFFSGDPDVVGHELTHGVTEFTSNLEY YGESGALNEAFSDIIGNDIDGTSWLLGDGIYTPNIPGDALRSLSDPTRFG QPDHYSNFYPDPNNDDEGGVHTNSGIINKAYYLLAQGGTSHGVTVTGIGR EAAVFIYYNAFTNYLTSTSNFSNARAAVIQAAKDFYGADSLAVTSAIQSF DAVGIK
Example 2.2
Expression of Paenibacillus sp. Metalloprotease PspPro2
[0347] The DNA sequence of the propeptide-mature form of PspPro2 was synthesized and inserted into the Bacillus subtilis expression vector p2JM103BBI (Vogtentanz, Protein Expr Purif, 55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX084 (AprE-PspPro2) (FIG. 2.1). Ligation of this gene encoding the PspPro2 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3' end of the Bacillus subtilis AprE signal sequence and the 5' end of the predicted PspPro2 native propeptide. The gene has an alternative start codon (GTG). As shown in FIG. 2.1, pGX084(AprE-PspPro2) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in B. subtilis, and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PspPro2, (SEQ ID NO: 9). The translation product of the synthetic AprE-PspPro2 gene is shown in SEQ ID NO: 10.
[0348] The pGX084(AprE-PspPro2) plasmid was transformed into B. subtilis cells (degU.sup.Hy32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm chloramphenicol and 1.2% skim milk (Cat#232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl2).
[0349] The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5) and 1 mM CaCl2 using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to a 150 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above, PspPro2 was eluted from the column with a linear salt gradient from 0 to 0.5 M NaCl in the loading buffer. The corresponding active fractions were collected, concentrated and buffer-exchanged again into the loading buffer described above. The sample was loaded onto a 20 ml DEAE Fast Flow column pre-equilibrated with the same loading buffer. PspPro2 was eluted from the column with a linear salt gradient from 0 to 0.3 M NaCl in the loading buffer. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses. The nucleotide sequence of the synthesized PspPro2 gene in plasmid pGX084 (AprE-PspPro2) is depicted in SEQ ID NO: 9. The sequence encoding the predicted native signal peptide is shown in italics and the oligo-nucleotide encoding the three residue addition (AGK) is shown in bold:
TABLE-US-00016 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAAT CTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCTGCTGGAAAAGCAG AGCATTCAGTTCCTGACCCGACGCAACTTACACCGACATTTCATGCTGAG CAGTGGAAGGCACCGAGCACGGTCACGGGCGACAACATCGTGTGGAGCTA CCTGAACAGACAGAAAAAGACGCTGCTGAACACGGACTCAACGAGCGTGA GAGACCAGTTCAGAATCATCGACAGAACGAGCGACAAGTCAGGCGCGTCA CATTATAGACTGAAGCAGTACGTGAACGGCATCCCGGTCTACGGAGCCGA GCAAACGATCCATGTGAATAATGCGGGCAAAGTTACATCATACCTGGGCG CCGTCATCTCAGAAGACCAGCAGCAAGATGCAACGGAGGATACAACACCG AAGATCAGCGCCACAGAAGCGGTCTATACGGCTTACGCCGAAGCGGCTGC AAGAATCCAGAGCTTCCCGTCAATTAATGACAGCCTGAGCGAAGCATCAG AGGAACAAGGCAGCGAGAACCAGGGCAATGAAATCCAAAACATCGGCATC AAGAGCAGCGTGTCAAACGACACGTATGCGGAGGCTCATAACAACGTTCT GCTGACACCGGTCGATCAGGCCGAACAGAGCTATATTGCAAAGATCGCGA ATCTGGAGCCGTCAGTCGAGCCGAAGGCCGAGCTGTATATCTATCCGGAC GGCGAGACGACGAGACTGGTGTACGTTACGGAGGTCAACATCCTTGAGCC TGCGCCGCTGAGAACAAGATACTTTATCGACGCCAAGACGGGCAAGATCG TGTTTCAGTACGATATCCTGAACCATGCGACGGGAACAGGCAGAGGCGTG GACGGCAAAACAAAATCATTCACGACAACGGCAAGCGGCAACAGATACCA GCTGAAGGACACAACAAGATCAAATGGCATCGTCACATACACGGCCGGAA ATAGACAGACGACGCCGGGAACGATTCTGACGGATACAGATAACGTGTGG GAAGATCCGGCAGCAGTTGATGCACATGCATACGCGATCAAGACGTACGA CTACTACAAGAACAAATTCGGAAGAGATTCAATCGATGGAAGAGGCATGC AAATCAGATCAACGGTTCATTATGGCAAAAAGTACAACAATGCCTTCTGG AACGGCAGCCAAATGACATACGGCGATGGAGACGGCTCAACGTTTACATT CTTTTCAGGCGACCCGGACGTCGTCGGCCATGAACTGACGCATGGCGTTA CAGAGTTCACGAGCAACCTGGAGTATTACGGCGAATCAGGCGCACTGAAT GAGGCTTTCAGCGACATCATTGGCAACGACATTGATGGCACATCATGGCT GCTTGGCGACGGCATTTACACACCTAACATTCCGGGCGATGCACTGAGAA GCCTGTCAGACCCTACGAGATTCGGCCAACCTGACCATTACAGCAACTTC TACCCGGATCCTAATAACGATGATGAGGGCGGAGTGCATACGAACAGCGG CATTATCAACAAAGCGTACTATCTGCTGGCACAAGGCGGAACGTCACATG GAGTGACGGTGACAGGAATCGGCAGAGAGGCGGCAGTGTTTATCTACTAC AACGCCTTCACAAACTACCTGACGAGCACGTCAAATTTCAGCAACGCTAG AGCGGCGGTCATCCAGGCAGCAAAGGACTTTTATGGAGCAGACTCACTGG CAGTTACGTCAGCAATTCAGTCATTCGACGCAGTTGGAATTAAG
[0350] The amino acid sequence of the PspPro2 precursor protein expressed from plasmid pGX084(AprE-PspPro2) is depicted in SEQ ID NO: 10. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text:
TABLE-US-00017 MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKAEHSVPDPTQLTPTFHAE QWKAPSTVTGDNIVWSYLNRQKKTLLNTDSTSVRDQFRIIDRTSDKSGAS HYRLKQYVNGIPVYGAEQTIHVNNAGKVTSYLGAVISEDQQQDATEDTTP KISATEAVYTAYAEAAARIQSFPSINDSLSEASEEQGSENQGNEIQNIGI KSSVSNDTYAEAHNNVLLTPVDQAEQSYIAKIANLEPSVEPKAELYIYPD GETTRLVYVTEVNILEPAPLRTRYFIDAKTGKIVFQYDILNHATGTGRGV DGKTKSFTTTASGNRYQLKDTTRSNGIVTYTAGNRQTTPGTILTDTDNVW EDPAAVDAHAYAIKTYDYYKNKFGRDSIDGRGMQIRSTVHYGKKYNNAFW NGSQMTYGDGDGSTFTFFSGDPDVVGHELTHGVTEFTSNLEYYGESGALN EAFSDIIGNDIDGTSWLLGDGIYTPNIPGDALRSLSDPTRFGQPDHYSNF YPDPNNDDEGGVHTNSGIINKAYYLLAQGGTSHGVTVTGIGREAAVFIYY NAFTNYLTSTSNFSNARAAVIQAAKDFYGADSLAVTSAIQSFDAVGIK
[0351] The amino acid sequence of the recombinant PspPro2 protein isolated from Bacillus subtilis culture was determined by tandem mass spectrometry, and shown below. It is the same as predicted and depicted in SEQ ID NO: 8.
TABLE-US-00018 ATGTGRGVDGKTKSFTTTASGNRYQLKDTTRSNGIVTYTAGNRQTTPGTI LTDTDNVWEDPAAVDAHAYAIKTYDYYKNKFGRDSIDGRGMQIRSTVHYG KKYNNAFWNGSQMTYGDGDGSTFTFFSGDPDVVGHELTHGVTEFTSNLEY YGESGALNEAFSDIIGNDIDGTSWLLGDGIYTPNIPGDALRSLSDPTRFG QPDHYSNFYPDPNNDDEGGVHTNSGIINKAYYLLAQGGTSHGVTVTGIGR EAAVFIYYNAFTNYLTSTSNFSNARAAVIQAAKDFYGADSLAVTSAIQSF DAVGIK
Example 2.3
Proteolytic Activity of Metalloprotease PspPro2
[0352] The proteolytic activity of purified PspPro2 was measured in 50 mM Tris (pH 7), using azo-casein (Cat#74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H2O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A440) using a SpectraMax 190. Net A440 was calculated by subtracting the A440 of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of duplicate assays, and the value varies no more than 5%. The proteolytic activity is shown as Net A440. The proteolytic assays with azo-casein as the substrate (FIG. 2.2) indicate that PspPro2 is an active protease.
Example 2.4
pH Profile of Metalloprotease PspPro2
[0353] With azo-casein as the substrate, the pH profile of PspPro2 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl diluted enzyme (500 ppm in Milli-Q H2O) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 2.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10 and 11. Each result was the mean of triplicate assays. As shown in FIG. 2.3, the optimal pH of PspPro2 is 7.5 with greater than 70% of its maximal activity retained between pH 5.5 and 9.5.
Example 2.5
Temperature Profile of Metalloprotease PspPro2
[0354] The temperature profile of PspPro2 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assay. The enzyme sample and azo-casein substrate were prepared as in Example 2.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H2O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20˜90° C.) for 5 min. After the incubation, 5 μl of diluted PspPro2 (200 ppm) or H2O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80 and 90° C. Each result was the mean of triplicate assays. The data in FIG. 2.4 suggest that PspPro2 showed an optimal temperature at 50° C., and retained greater than 70% of its maximal activity between 40 and 65° C.
Example 2.6
Cleaning Performance of Metalloprotease PspPro2 in Automatic Dishwashing (ADW) Conditions
[0355] The cleaning performance of PspPro2 protein in automatic dishwashing (ADW) conditions was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified PspPro2 protein samples were diluted with the dilution solution containing 10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca2+:Mg2+=3:1), in the presence of a bleach component (Peracid N,N-phthaloylaminoperoxycaproic acid-PAP) (AT detergent composition shown in Table 1). To initiate the reaction, 180 μl of the AT detergent solution at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the "Initial performance") using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as "Wash-off"). The sum of two absorbance measurements ("Initial performance" plus "Wash-off") gives the "Total performance", which measures the protease activity on the model stain. Dose response for cleaning of PA-S-38 microswatches at pH 6 and pH 8 for PspPro2 in AT detergent in the presence of bleach, is shown in FIGS. 2.5A and 2.5B, respectively.
TABLE-US-00019 TABLE 2.1 Composition of AT dish detergent with bleach Concentration Ingredient (mg/ml) MGDA (methlyglycinediacetic acid) 0.143 Sodium citrate 1.86 Citric acid* varies PAP (peracid N,N-phthaloylaminoperoxycaproic acid) 0.057 Plurafac ® LF 18B (a non-ionic surfactant) 0.029 Bismuthcitrate 0.006 Bayhibit ® S (Phosphonobutantricarboxylic acid 0.006 sodium salt) Acusol ® 587 (a calcium polyphosphate inhibitor) 0.029 PEG 6000 0.043 PEG 1500 0.1 *The pH of the AT formula detergent is adjusted to the desired pH value (pH 6 or 8) by the addition of 0.9M citric acid.
Example 2.7
Cleaning Performance of Metalloprotease PspPro2 in Laundry Conditions
A. Cleaning Performance in Liquid Laundry Detergent
[0356] The cleaning performance of PspPro2 protein in liquid laundry detergent was tested using EMPA-116 (cotton soiled with blood/milk/ink) microswatches (obtained from CFT Vlaardingen, The Netherlands) at pH 8.2 using a commercial detergent. Prior to the reaction, purified PspPro2 protein samples were diluted with a dilution solution (10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol) to the desired concentrations; and the commercial detergent (Tide®, Clean Breeze®, Proctor & Gamble, USA, purchased September 2011) was incubated at 95° C. for 1 hour to inactivate the enzymes present in the detergent. Proteolytic assays were subsequently performed to confirm the inactivation of proteases in the commercial detergent. The heat treated detergent was further diluted with 5 mM HEPES (pH 8.2) to a final concentration of 0.788 g/L. Meanwhile, the water hardness of the buffered liquid detergent was adjusted to 103 ppm (Ca2+:Mg2+=3:1). The specific conductivity of the buffered detergent was adjusted to either 0.62 mS/cm (low conductivity) or 3.5 mS/cm (high conductivity) by adjusting the NaCl concentration in the buffered detergent. To initiate the reaction, 190 μl of either the high or low conductivity buffered detergent was added to a 96-MTP containing the EMPA-116 microswatches, followed by the addition of 10 μl of diluted enzyme (or the dilution solution as blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 20 min at 32° C. and 1150 rpm. After incubation, 150 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 600 nm using a spectrophotometer, which indicates the protease activity on the model stain; and Net A600 was subsequently calculated by subtracting the A600 of the blank control from that of the enzyme. Dose response for the cleaning of EMPA-116 microswatches in liquid laundry detergent at high or low conductivity is shown in FIG. 2.6A.
B. Cleaning Performance in Powder Laundry Detergent
[0357] The cleaning performance of PspPro2 protein in powder laundry detergent was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) using a commercial detergent. Prior to the reaction, purified PspPro2 protein samples were diluted with a dilution solution (10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol) to the desired concentrations. The powder laundry detergent (Tide®, Bleach Free, Proctor & Gamble, China, purchased in December 2011) was dissolved in water with 103 ppm water hardness (Ca2+:Mg2+=3:1) to a final concentration of 2 g/L (with conductivity of 2.3 mS/cm-low conductivity) or 5 g/L (with conductivity of 5.5 mS/cm-high conductivity). The detergents of different conductivities were subsequently heated in a microwave to near boiling in order to inactivate the enzymes present in the detergent. Proteolytic activity was measured following treatment to ensure that proteases in the commercial detergent had been inactivated. To initiate the reaction, 190 μl of either the high or low conductivity heat-treated detergent was added to a 96-MTP containing the PA-S-38 microswatches, followed by the addition of 10 μl of diluted enzyme (or the dilution solution as blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 15 minutes at 32° C. and 1150 rpm. After incubation, 150 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm using a spectrophotometer, which indicates the protease activity on the model stain; and Net A405 was subsequently calculated by subtracting the A405 of the blank control from that of the enzyme. Dose response for the cleaning of PA-S-38 microswatches in powder laundry detergent at high or low conductivity is shown in FIG. 2.6B.
Example 2.8
Comparison of PspPro2 to Other Metalloproteases
Identification of Homologous Proteases
[0358] Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The mature protein amino acid sequence for PspPro2 (SEQ ID NO: 8) is used as query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 2.2A and 2.2B provide a list of sequences with the percent identity to PspPro2. The length in Table 2.2 refers to the entire sequence length of the homologous proteases.
TABLE-US-00020 TABLE 2.2A List of sequences with percent identity to PspPro2 protein identified from the NCBI non-redundant protein database PID to Accession # PspPro2 Organism Length AAB02774.1 55 Geobacillus stearothermophilus 552 AAA22623.1 56 Bacillus caldolyticus 544 P00800 56 Bacillus thermoproteolyticus 548 YP_003670279.1 57 Geobacillus sp. C56-T3 546 BAD60997.1 57 Bacillus megaterium 562 ZP_02326503.1 58 Paenibacillus larvae subsp. larvae BRL-230010 520 ZP_08640523.1 58 Brevibacillus laterosporus LMG 15441 564 YP_003597483.1 58 Bacillus megaterium DSM 319 562 ZP_09069025.1 59 Paenibacillus larvae subsp. larvae B-3650 520 YP_001373863.1 59 Bacillus cytotoxicus NVH 391-98 565 ZP_04149724.1 59 Bacillus pseudomycoides DSM 12442 566 CAA43589.1 60 Brevibacillus brevis 527 ZP_10738945.1 60 Brevibacillus sp. CF112 528 ZP_04216147.1 60 Bacillus cereus Rock3-44 566 ZP_10575942.1 61 Brevibacillus sp. BC25 528 YP_002770810.1 62 Brevibacillus brevis NBRC 100599 528 ZP_08511445.1 63 Paenibacillus sp. HGF7 525 ZP_09077634.1 64 Paenibacillus elgii B69 524 ZP_09071078.1 67 Paenibacillus larvae subsp. larvae B-3650 529 YP_003872180.1 73 Paenibacillus polymyxa E681 587 YP_005073223.1 78 Paenibacillus terrae HPL-003 591 ZP_09775364.1 78 Paenibacillus sp. Aloe-11 593 YP_003948511.1 80 Paenibacillus polymyxa SC2 592 YP_005073224.1 94 Paenibacillus terrae HPL-003 595 ZP_10241029.1 96 Paenibacillus peoriae KCTC 3763 599 ZP_09775365.1 100 Paenibacillus sp. Aloe-11 580
TABLE-US-00021 TABLE 2.2B List of sequences with percent identity to PspPro2 protein identified from the Genome Quest database PID to Patent # PspPro2 Organism Length JP2002272453-0002 57.01 Bacillus megaterium 562 US6518054-0001 57.19 Bacillus sp. 319 EP2390321-0177 57.19 Bacillus caldolyticus 544 US20120107907-0176 57.19 Bacillus stearothermophilis 548 WO9520663-0003 57.51 empty 319 WO2012110562-0003 57.51 Geobacillus 319 stearothermophilus WO2012110563-0002 57.51 Bacillus caldolyticus 319 WO2004011619-0056 57.51 empty 546 WO2004011619-0003 57.51 empty 546 JP2002272453-0003 57.64 empty 562 US6518054-0002 57.88 Bacillus sp. 316 EP2178896-0184 58.15 Bacillus anthracis 566 WO2012110563-0004 58.28 Bacillus megaterium 320 JP1995184649-0001 58.79 Lactobacillus sp. 566 JP1994014788-0003 58.84 empty 317 US8114656-0178 59.42 Bacillus thuringiensis 566 WO2012110562-0005 59.49 Bacillus cereus 320 US5962264-0004 59.81 empty 566 US20120107907-0185 59.81 Bacillus cereus 317 US20120107907-0179 59.81 Bacillus cereus 566 WO2012110563-0005 60.13 Bacillus cereus 320 EP2390321-0186 60.33 Bacillus brevis 304 JP2005229807-0018 78.62 Paenibacillus polymyxa 566 EP2390321-0187 79.21 Bacillus polymyxa 302
B. Alignment of Homologous Protease Sequences
[0359] The amino acid sequence of mature PspPro2 (SEQ ID NO: 8) was aligned with thermolysin (P00800, Bacillus thermoproteolyticus) and protease from Paenibacillus sp. Aloe-11 (ZP_09775365.1) sequences using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters. FIG. 2.7 shows the alignment of PspPro2 with these protease sequences.
C. Phylogenetic Tree
[0360] A phylogenetic tree for precursor PspPro2 protein sequence (SEQ ID NO: 7) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. Mol Biol. Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in FIG. 2.8.
Example 3.1
Cloning of Paenibacillus Humicus Metalloprotease PhuPro2
[0361] A strain (DSM18784) of Paenibacillus humicus was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the Paenibacillus humicus strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina's next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in Paenibacillus humicus encodes a metalloprotease and the sequence of this gene, called PhuPro2, is provided in SEQ ID NO: 11. The corresponding protein encoded by the PhuPro2 gene is shown in SEQ ID NO: 12. At the N-terminus, the protein has a signal peptide with a length of 23 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PhuPro2 is a secreted enzyme. The propeptide region was predicted based on its protein sequence alignment with the Paenibacillus polymyxa Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PhuPro2 protein in shown in SEQ ID NO: 13. The nucleotide sequence of the PhuPro2 gene isolated from Paenibacillus humicus is set forth as SEQ ID NO: 11. The sequence encoding the predicted native signal peptide is shown in italics:
TABLE-US-00022 ATGAAAAAAATGATTCCTACTCTGCTCGGTACCGTATTGCTGCTTTCTTC CGCTTCCGCTGTCGCTGCTGAATCGCCAAGCCTCGGAGCGGCCGGAACTC CCGGGGTCAGCGTCGTGAACAATCAGCTCGTGACTCAATTCATCGAGGCT TCCAAGGATGCCAAGATTGTCCCGGGCTCTTCCGAGGATAAAATCTGGGC TTTCCTTGAAGGCCAGCAAGCAAAGCTGGGTGTATCCGCAGCGGATGTAA AAACCTCGTTCCTGATCCAGAAGAAGGAAGTCGATCCGACTTCGGGCGTC GAGCATTTCCGCCTGCAGCAATATGTGAATGGCATCCCGGTATATGGCGG TGACCAAACCATTCACATCGACAAGGCCGGCCAGGTTACGTCGTTCGTAG GAGCTGTTCTGCCGGCTCAAAATCAAATCACGGCAAAATCCAGCGTACCA GCCATAAGCGCATCCGACGCTCTGGCTATCGCGGCGAAGGAAGCCAGTTC CCGCATCGGCGAGCTGGGAGCACAGGAGAAGACTCCGTCGGCTCAGCTGT ACGTATATCCGGAAGGCAACGGGTCGCGTCTCGTCTACCAGACGGAAGTG AATGTGCTTGAGCCGCAGCCTCTGCGCACCCGCTATCTTATCGATGCGGC CGACGGCCATATCGTGCAGCAGTACGATCTGATCGAGACGGCGACCGGTT CGGGCACGGGCGTGCTGGGCGACAATAAGACGTTCCAGACGACTCTTTCC GGCAGCACGTACCAGCTGAAAGACACCACTCGCGGCAACGGCATCTACAC CTACACAGCCAGCAATCGGACCACGATTCCGGGCACGCTGCTGACGGACG CCGACAACGTATGGACGGATGGAGCCGCCGTCGATGCCCATACTTATGCC GGAAAAGTATATGATTTCTACAAAACGAAGTTCGGACGCAACAGCCTCGA CGGCAACGGCCTGCTGATCCGTTCCTCGGTCCACTACAGCAGCAGGTACA ACAATGCCTTCTGGAACGGCACCCAGATTGTATTCGGCGACGGCGACGGC TCGACGTTCATTCCGCTGTCGGGCGATCTCGACGTGGTCGGCCATGAGCT GTCCCACGGAGTCATCGAGTACACGTCCAACCTTCAATACCTCAATGAAT CCGGCGCGCTGAACGAGTCCTATGCCGACGTCCTCGGCAACTCGATCCAG GCGAAAAACTGGCTTATCGGCGACGATGTCTATACGCCTGGCATCTCCGG AGATGCTCTCCGTTCCATGTCCAACCCGACGCTTTACGGGCAGCCGGACA ACTATGCCAACCGCTATACGGGATCTTCCGACAACGGCGGCGTTCATACG AACAGCGGCATCACGAACAAAGCGTTCTACCTGCTCGCCCAAGGCGGCAC CCAGAACGGCGTTACCGTCGCCGGCATCGGGCGCGACGCAGCCGTGAACA TTTTCTACAACACAGTGGCCTATTACCTTACTTCCACTTCCAACTTCGCC GCGGCGAAGAACGCCTCGATCCAGGCAGCCAAAGACCTGTACGGAACGGG CTCCTCTTATGTCACCTCGGTGACCAATGCATTCAGAGCCGTAGGCCTG
[0362] The amino acid sequence of the PhuPro2 precursor protein is set forth as SEQ ID NO: 12. The predicted signal sequence is shown in italics, and the predicted propeptide is shown in underlined text:
TABLE-US-00023 MKKMIPTLLGTVLLLSSASAVAAESPSLGAAGTPGVSVVNNQLVTQFIEA SKDAKIVPGSSEDKIWAFLEGQQAKLGVSAADVKTSFLIQKKEVDPTSGV EHFRLQQYVNGIPVYGGDQTIHIDKAGQVTSFVGAVLPAQNQITAKSSVP AISASDALAIAAKEASSRIGELGAQEKTPSAQLYVYPEGNGSRLVYQTEV NVLEPQPLRTRYLIDAADGHIVQQYDLIETATGSGTGVLGDNKTFQTTLS GSTYQLKDTTRGNGIYTYTASNRTTIPGTLLTDADNVWTDGAAVDAHTYA GKVYDFYKTKFGRNSLDGNGLLIRSSVHYSSRYNNAFWNGTQIVFGDGDG STFIPLSGDLDVVGHELSHGVIEYTSNLQYLNESGALNESYADVLGNSIQ AKNWLIGDDVYTPGISGDALRSMSNPTLYGQPDNYANRYTGSSDNGGVHT NSGITNKAFYLLAQGGTQNGVTVAGIGRDAAVNIFYNTVAYYLTSTSNFA AAKNASIQAAKDLYGTGSSYVTSVTNAFRAVGL
[0363] The amino acid sequence of the predicted mature form of PhuPro2 is set forth as SEQ ID NO: 13:
TABLE-US-00024 ATGSGTGVLGDNKTFQTTLSGSTYQLKDTTRGNGIYTYTASNRTTIPGTL LTDADNVWTDGAAVDAHTYAGKVYDFYKTKFGRNSLDGNGLLIRSSVHYS SRYNNAFWNGTQIVFGDGDGSTFIPLSGDLDVVGHELSHGVIEYTSNLQY LNESGALNESYADVLGNSIQAKNWLIGDDVYTPGISGDALRSMSNPTLYG QPDNYANRYTGSSDNGGVHTNSGITNKAFYLLAQGGTQNGVTVAGIGRDA AVNIFYNTVAYYLTSTSNFAAAKNASIQAAKDLYGTGSSYVTSVTNAFRA VGL
Example 3.2
Expression of Paenibacillus humicus s Metalloprotease PhuPro2
[0364] The DNA sequence of the propeptide-mature form of PhuPro2 was synthesized and inserted into the Bacillus subtilis expression vector p2JM103BBI (Vogtentanz, Protein Expr Purif, 55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX150(AprE-PhuPro2) (FIG. 1). Ligation of this gene encoding the PhuPro2 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3' end of the B. subtilis AprE signal sequence and the 5' end of the predicted PhuPro2 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in FIG. 1 was labeled pGX150(AprE-PhuPro2). As shown in FIG. 3.1, pGX150(AprE-PhuPro2) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in B. subtilis, and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PhuPro2 (SEQ ID NO: 14). The translation product of the synthetic AprE-PhuPro2 gene is shown in SEQ ID NO: 15.
[0365] The pGX150 (AprE-PhuPro2) plasmid was then transformed into B. subtilis cells (degU.sup.Hy32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat#232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl2).
[0366] The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl2 and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above; and the active flow-through fractions were collected and concentrated. The sample was loaded onto a 320 ml Superdex 75 gel filtration column pre-equilibrated with the loading buffer described above containing 0.15 M NaCl. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses.
[0367] The nucleotide sequence of the synthesized PhuPro2 gene in plasmid pGX150(AprE-PhuPro2) is depicted in SEQ ID NO: 14. The sequence encoding the three residue addition (AGK) is shown in bold:
TABLE-US-00025 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAAT CTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCTGCTGGAAAAGAAT CACCGAGCCTTGGCGCTGCAGGAACACCGGGCGTTAGCGTTGTGAATAAC CAACTGGTCACGCAGTTCATCGAAGCATCAAAAGACGCGAAAATTGTCCC TGGATCAAGCGAAGATAAGATTTGGGCATTTCTGGAAGGCCAGCAAGCAA AGCTTGGCGTCTCAGCTGCCGACGTGAAGACGAGCTTCCTGATCCAGAAG AAGGAGGTTGACCCGACATCAGGCGTTGAGCACTTTAGACTGCAACAGTA CGTCAACGGCATCCCGGTTTATGGAGGCGATCAAACAATCCATATTGATA AGGCAGGCCAGGTCACATCATTCGTCGGAGCTGTCCTGCCGGCTCAGAAC CAAATTACAGCAAAATCATCAGTTCCGGCAATTTCAGCCTCAGACGCTCT GGCAATCGCTGCCAAGGAGGCAAGCTCAAGAATTGGCGAACTGGGCGCAC AAGAAAAGACACCGAGCGCCCAACTTTATGTCTATCCGGAGGGCAACGGA AGCAGACTGGTGTACCAGACAGAGGTCAATGTTCTGGAGCCGCAACCGCT GAGAACGAGATACCTTATCGATGCTGCGGATGGCCACATTGTTCAGCAAT ACGACCTGATTGAGACAGCAACAGGAAGCGGAACGGGCGTGCTGGGCGAC AACAAGACGTTTCAGACAACACTTAGCGGCAGCACGTACCAACTTAAGGA CACGACGAGAGGCAATGGCATTTACACGTACACGGCCTCAAACAGAACGA CAATCCCAGGCACACTGCTGACGGATGCAGACAATGTTTGGACGGACGGC GCAGCAGTTGACGCACACACGTACGCCGGCAAGGTGTACGACTTTTACAA GACGAAGTTCGGCAGAAACAGCCTTGATGGAAATGGACTGCTGATCAGAA GCAGCGTCCACTACAGCAGCAGATACAATAACGCCTTCTGGAACGGCACA CAAATCGTCTTTGGCGATGGAGACGGATCAACATTCATCCCGCTGTCAGG CGACCTGGACGTTGTGGGCCACGAGCTGAGCCACGGCGTCATCGAGTACA CGAGCAACCTGCAGTACCTGAATGAAAGCGGCGCACTGAACGAGTCATAT GCTGATGTGCTTGGCAATAGCATCCAGGCCAAGAACTGGCTTATCGGAGA CGACGTCTACACACCTGGCATCAGCGGCGATGCTCTGAGAAGCATGAGCA ATCCTACACTTTACGGCCAACCGGACAACTACGCGAATAGATATACGGGC AGCAGCGACAATGGCGGCGTTCATACAAACTCAGGCATCACGAACAAGGC GTTCTACCTGCTGGCACAGGGAGGCACGCAAAACGGCGTTACAGTTGCGG GCATTGGCAGAGATGCGGCCGTCAACATCTTCTACAACACAGTCGCCTAC TACCTGACGAGCACGTCAAACTTCGCAGCGGCAAAGAACGCATCAATTCA AGCAGCAAAGGATCTGTACGGAACAGGCAGCTCATATGTCACGTCAGTTA CGAATGCGTTTAGAGCCGTCGGCCTTTAA
[0368] The amino acid sequence of the PhuPro2 precursor protein expressed from plasmid pGX150(AprE-PhuPro2) is depicted in SEQ ID NO: 15. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted propeptide is shown in underlined text.
TABLE-US-00026 (SEQ ID NO: 15) MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKESPSLGAAGTPGVSVVNN QLVTQFIEASKDAKIVPGSSEDKIWAFLEGQQAKLGVSAADVKTSFLIQK KEVDPTSGVEHFRLQQYVNGIPVYGGDQTIHIDKAGQVTSFVGAVLPAQN QITAKSSVPAISASDALAIAAKEASSRIGELGAQEKTPSAQLYVYPEGNG SRLVYQTEVNVLEPQPLRTRYLIDAADGHIVQQYDLIETATGSGTGVLGD NKTFQTTLSGSTYQLKDTTRGNGIYTYTASNRTTIPGTLLTDADNVWTDG AAVDAHTYAGKVYDFYKTKFGRNSLDGNGLLIRSSVHYSSRYNNAFWNGT QIVFGDGDGSTFIPLSGDLDVVGHELSHGVIEYTSNLQYLNESGALNESY ADVLGNSIQAKNWLIGDDVYTPGISGDALRSMSNPTLYGQPDNYANRYTG SSDNGGVHTNSGITNKAFYLLAQGGTQNGVTVAGIGRDAAVNIFYNTVAY YLTSTSNFAAAKNASIQAAKDLYGTGSSYVTSVTNAFRAVGL.
Example 3.3
Proteolytic Activity of Metalloprotease PhuPro2
[0369] The proteolytic activity of purified metalloprotease PhuPro2 was measured in 50 mM Tris (pH 7), using azo-casein (Cat#74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H2O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A440) using a SpectraMax 190. Net A440 was calculated by subtracting the A440 of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays.
[0370] The proteolytic activity is shown as Net A440. The proteolytic assay with azo-casein as the substrate (shown in FIG. 3.2) indicates that PhuPro2 is an active protease.
Example 3.4
pH Profile of Metalloprotease PhuPro2
[0371] With azo-casein as the substrate, the pH profile of metalloprotease PhuPro2 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 ml Milli-Q H2O diluted enzyme (125 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 3.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in FIG. 3.3, the optimal pH of PhuPro2 is 6, with greater than 70% of maximal activity retained between 5.5 and 8.5.
Example 3.5
Temperature Profile of Metalloprotease PhuPro2
[0372] The temperature profile of metalloprotease PhuPro2 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 3.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H2O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20˜90° C.) for 5 min. After the incubation, 5 μl of diluted enzyme (50 ppm) or H2O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 3.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in FIG. 3.4 suggests that PhuPro2 showed an optimal temperature at 50° C., and retained greater than 70% of its maximum activity between 45 and 65° C.
Example 3.6
Cleaning Performance of Metalloprotease PhuPro2
[0373] The cleaning performance of PhuPro2 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca2+:Mg2+=3:1) (detergent composition shown in Table 3.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the "Initial performance") using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the "Wash-off"). The sum of two absorbance measurements ("Initial performance" plus "Wash-off") gives the "Total performance", which measures the protease activity on the model stain; and Net A405 was subsequently calculated by subtracting the A405 of the "Total performance" of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 in AT dish detergent for PhuPro2 is shown in FIGS. 3.5A and 3.5B.
TABLE-US-00027 TABLE 3.1 Composition of AT dish detergent Concentration Ingredient (mg/ml) MGDA (methylglycinediacetic acid) 0.143 Sodium citrate 1.86 Citric acid* varies Plurafac ® LF 18B (a non-ionic surfactant) 0.029 Bismuthcitrate 0.006 Bayhibit ® S (Phosphonobutantricarboxylic 0.006 acid sodium salt) Acusol ® 587 (a calcium polyphosphate inhibitor) 0.029 PEG 6000 0.043 PEG 1500 0.1 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid.
Example 3.7
Comparison of PhuPro2 to Other Proteases
A. Identification of Homologous Proteases
[0374] Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PhuPro2 (SEQ ID NO: 13) is used as the query sequences. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 3.2A and 3.2B provide a list of sequences with the percent identity to PhuPro2. The length in Table 3.2 refers to the entire sequence length of the homologous proteases.
TABLE-US-00028 TABLE 3.2A List of sequences with percent identity to PhuPro2 protein identified from the NCBI non-redundant protein database PID to Accession # PhuPro2 Organism Length P00800 59 Bacillus thermoproteolyticus 548 YP_003872180.1 59 Paenibacillus polymyxa E681 587 ZP_10575942.1 59 Brevibacillus sp. BC25 528 ZP_02326602.1 60 Paenibacillus larvae subsp. 520 larvae BRL-230010 ADM87306.1 61 Bacillus megaterium 562 ZP_09069025.1 61 Paenibacillus larvae subsp. 520 larvae B-3650 ZP_09069194.1 62 Paenibacillus larvae subsp. 502 Larvae B-3650 ZP_10738945.1 63 Brevibacillus sp. CF112 528 ZP_08511445.1 64 Paenibacillus sp. HGF7 525 ZP_09077634.1 65 Paenibacillus elgii B69 524 ZP_09775365.1 65 Paenibacillus sp. Aloe-11 580 ZP_09775364.1 70 Paenibacillus sp. Aloe-11 593 P29148 71 Paenibacillus polymyxa 590 ZP_10241030.1 71 Paenibacillus peoriae KCTC 593 3763 ZP_09071078.1 71 Paenibacillus larvae subsp. 529 larvae B-3650 YP_003872179.1 72 Paenibacillus polymyxa E681 592 YP_005073223.1 72 Paenibacillus terrae HPL-003 591
TABLE-US-00029 TABLE 3.2B List of sequences with percent identity to PhuPro2 protein identified from the Genome Quest Patent database PID to Patent ID # PhuPro2 Organism Length US20090208474- 59.22 Bacillus thermoproteolyticus 316 0030 JP2002272453-0002 59.42 Bacillus megaterium 562 JP2006124323-0003 59.55 Bacillus thermoproteolyticus 316 US8114656-0183 59.87 Bacillus stearothermophilis 316 JP1989027475-0001 59.87 Bacillus subtilis 316 US20120009651- 59.87 Geobacillus 548 0002 caldoproteolyticus JP2002272453-0003 60.45 empty 562 WO2012110563- 60.77 Bacillus megaterium 320 0004 EP2390321-0186 62.25 Bacillus brevis 304 JP2005229807-0018 71.85 Paenibacillus polymyxa 566 US8114656-0187 72.09 Bacillus polymyxa 302
B. Alignment of Homologous Protease Sequences
[0375] The amino acid sequence of predicted mature PhuPro2 (SEQ ID NO: 13) protein was aligned with thermolysin (P00800, Bacillus thermoproteolyticus), and protease from Paenibacillus terrae HPL-003 (YP_005073223.1) sequences using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters. FIG. 3.6 shows the alignment of PhuPro2 with these protease sequences.
C. Phylogenetic Tree
[0376] A phylogenetic tree for full length sequence of PhuPro2 (SEQ ID NO: 12) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. Mol Biol. Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in FIG. 3.7.
Example 4.1
Cloning of Paenibacillus ehimensis Metalloprotease PehPro1
[0377] A strain (DSM11029) of Paenibacillus ehimensis was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the Paenibacillus ehimensis strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina's next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in Paenibacillus ehimensis encodes a metalloprotease and the sequence of this gene, called PehPro1, is provided in SEQ ID NO: 16. The corresponding protein encoded by the PehPro1 gene is shown in SEQ ID NO: 17. At the N-terminus, the protein has a signal peptide with a length of 23 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PehPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the Paenibacillus polymyxa Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PehPro1 protein is shown in SEQ ID NO: 18.
[0378] The nucleotide sequence of the PehPro1 gene isolated from Paenibacillus ehimensis is set forth as SEQ ID NO: 16. The sequence encoding the predicted native signal peptide is shown in italics:
TABLE-US-00030 ATGTTAAAAGTATGGGCATCGATTATTACAGGAGCATTTTTGCTCGGGAG CGTGCAAGGGGTGCAAGCTGCTCCACAAGATCAAGCTGCTCCCTTCGGAG GATTCACCCCTCAATTGATTACCGGGGAAAGCTGGAGTGCGCCGCAAGGA GTATCGGGAGAGGAAAAAATCTGGAAGTATCTCGAATCCAAGCAGGAAAG CTTCCAAATCGGCCAAACCGTTGATCTGAAAAAGCAATTGAAAATTATCG GCCAAACGACCGACGAGAAAACGGGAACCACGCATTACCGTCTACAGCAG TATGTGGGAGGCGTCCCCGTATACGGCGGCGTACAAACGATCCATGTCAA CAAAGAAGGACAAGTTACCTCGCTGATCGGCAGCCTGCTTCCCGACCAGC AGCAGCAAGTTTCGAAAAGCTTGAATTCGCAAATCAGCGAAGCGCAAGCC ATCGCCGTGGCCCAGAAAGATACCGAGGCCGCCGTCGGCAAGCTGGGTGA ACCGCAAAAGACACCGGAAGCGGATCTGTACGTTTATTTACACAACGGAC AACCGGTCCTCGCTTATGTGACCGAGGTTAACGTTCTCGAACCGGAGGCA ATCCGGACGCGCTACTTCATCAGCGCCGAAGACGGCAGCATTTTATTCAA GTACGACATCCTCGCTCACGCTACAGGTACCGGAAAAGGCGTGCTCGGAG ATACGAAATCGTTCACGACCACGCAATCCGGCTCCACTTATCAATTGAAG GATACGACGCGCGGGCAAGGTATCGTCACTTACAGCGCTGGCAACCGGTC CTCTCTGCCGGGAACGCTGCTCACCAGCTCCAGCAATATTTGGAACGACG GCGCGGCGGTCGATGCGCATGCCTATACCGCCAAAGTGTACGATTACTAT AAAAACAAATTTGGCCGCAACAGCATTGACGGCAACGGCTTCCAGCTTAA ATCGACCGTGCACTATTCCTCCAGATACAACAACGCCTTCTGGAACGGTG TGCAAATGGTGTACGGCGACGGCGACGGCGTAACCTTCATTCCGTTCTCC GCCGATCCGGACGTCATCGGCCACGAATTGACCCACGGCGTTACGGAACA TACGGCCGGCCTGGAATACTACGGCGAATCCGGAGCGCTGAACGAATCGA TCTCCGATATTATCGGCAACGCGATCGACGGCAAAAACTGGCTGATCGGC GACTTGATTTATACGCCGAATACTCCCGGGGACGCCCTCCGCTCTATGGA GAACCCCAAGCTGTATAACCAACCCGACCGCTATCAAGACCGCTATACGG GACCTTCCGATAACGGCGGCGTGCATATTAACAGCGGTATCAACAACAAA GCCTTCTACCTGATCGCCCAAGGCGGCACGCACTATGGCGTCACCGTGAA CGGGATCGGACGCGATGCGGCTGTGCAAATTTTCTATGACGCCCTCATCA ATTACCTGACTCCAACTTCGAACTTCTCGGCGATGCGCGCAGCAGCCATT CAAGCGGCAACCGACCTGTACGGAGCGAATTCTTCTCAAGTAAACGCTGT CAAAAAAGCGTATACTGCCGTCGGCGTGAAC
[0379] The amino acid sequence of the PehPro1 precursor protein is set forth as SEQ ID NO: 17. The predicted signal sequence is shown in italics, and the predicted propeptide is shown in underlined text:
TABLE-US-00031 MLKVWASIITGAFLLGSVQGVQAAPQDQAAPFGGFTPQLITGESWSAPQG SGEEKIWKYLESKQESFQIGQTVDLKKQLKIIGQTTDEKTGTTHYRLQQ YVGGVPVYGGVQTIHVNKEGQVTSLIGSLLPDQQQQVSKSLNSQISEAQA IAVAQKDTEAAVGKLGEPQKTPEADLYVYLHNGQPVLAYVTEVNVLEPEA IRTRYFISAEDGSILFKYDILAHATGTGKGVLGDTKSFTTTQSGSTYQLK DTTRGQGIVTYSAGNRSSLPGTLLTSSSNIWNDGAAVDAHAYTAKVYDYY KNKFGRNSIDGNGFQLKSTVHYSSRYNNAFWNGVQMVYGDGDGVTFIPFS ADPDVIGHELTHGVTEHTAGLEYYGESGALNESISDIIGNAIDGKNWLIG DLIYTPNTPGDALRSMENPKLYNQPDRYQDRYTGPSDNGGVHINSGINNK AFYLIAQGGTHYGVTVNGIGRDAAVQIFYDALINYLTPTSNFSAMRAAAI QAATDLYGANSSQVNAVKKAYTAVGVN
[0380] The amino acid sequence of the predicted mature form of PehPro1 is set forth as SEQ ID NO: 18:
TABLE-US-00032 ATGTGKGVLGDTKSFTTTQSGSTYQLKDTTRGQGIVTYSAGNRSSLPGTL LTSSSNIWNDGAAVDAHAYTAKVYDYYKNKFGRNSIDGNGFQLKSTVHYS SRYNNAFWNGVQMVYGDGDGVTFIPFSADPDVIGHELTHGVTEHTAGLEY YGESGALNESISDIIGNAIDGKNWLIGDLIYTPNTPGDALRSMENPKLYN QPDRYQDRYTGPSDNGGVHINSGINNKAFYLIAQGGTHYGVTVNGIGRDA AVQIFYDALINYLTPTSNFSAMRAAAIQAATDLYGANSSQVNAVKKAYTA VGVN
Example 4.2
Expression of Paenibacillus ehimensis Metalloprotease PehPro1
[0381] The DNA sequence of the propeptide-mature form of PehPro1 was synthesized and inserted into the Bacillus subtilis expression vector p2JM103BBI (Vogtentanz, Protein Expr Purif, 55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX148(AprE-PehPro1) (FIG. 4.1). Ligation of this gene encoding the PehPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3' end of the B. subtilis AprE signal sequence and the 5' end of the predicted PehPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in FIG. 1 was labeled pGX148(AprE-PehPro1). As shown in FIG. 1, pGX148(AprE-PehPro1) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in B. subtilis, and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PehPro1 (SEQ ID NO: 19). The translation product of the synthetic AprE-PehPro1 gene is shown in SEQ ID NO: 20.
[0382] The pGX148(AprE-PehPro1) plasmid was then transformed into B. subtilis cells (degU.sup.Hy32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat#232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl2).
[0383] The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl2 and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above, PehPro1 was eluted from the column with a linear salt gradient from 0 to 0.3 M NaCl in the loading buffer. The corresponding active fractions were collected, concentrated and buffer-exchanged again into the loading buffer described above. The sample was loaded onto a 40 ml DEAE Fast Flow column pre-equilibrated with the same loading buffer. PehPro1 was eluted from the column with a linear salt gradient from 0 to 0.15 M NaCl in the loading buffer. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses.
[0384] The nucleotide sequence of the synthesized PehPro1 gene in plasmid pGX148(AprE-PehPro1) is depicted in SEQ ID NO: 19. The sequence encoding the three residue addition (AGK) is shown in bold:
TABLE-US-00033 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAAT CTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCTGCTGGAAAAGCAC CTCAAGATCAGGCAGCACCTTTTGGAGGCTTTACACCGCAACTTATCACA GGCGAATCATGGTCAGCACCGCAGGGCGTTTCAGGCGAGGAAAAGATCTG GAAGTACCTTGAGAGCAAGCAGGAGTCATTTCAAATCGGCCAGACAGTCG ACCTGAAAAAGCAACTGAAGATCATCGGCCAAACAACGGACGAAAAGACG GGCACGACGCATTATAGACTGCAACAATATGTTGGCGGCGTGCCGGTTTA TGGAGGCGTGCAAACAATCCACGTGAACAAGGAAGGACAGGTCACGTCAC TGATCGGCAGCCTGCTGCCGGATCAGCAGCAACAAGTCTCAAAGAGCCTG AACTCACAAATTAGCGAGGCACAAGCGATTGCAGTTGCACAAAAGGACAC GGAAGCAGCTGTCGGCAAGCTGGGCGAACCGCAAAAAACACCTGAGGCTG ACCTTTACGTCTACCTGCATAACGGCCAGCCGGTCCTTGCGTACGTTACG GAAGTTAACGTGCTGGAGCCGGAGGCCATCAGAACGAGATACTTCATTAG CGCGGAGGATGGAAGCATTCTGTTTAAGTACGATATTCTTGCTCACGCGA CAGGCACAGGCAAGGGCGTCCTTGGCGACACAAAAAGCTTCACGACAACG CAGAGCGGATCAACGTACCAGCTGAAAGATACAACAAGAGGACAAGGCAT CGTTACGTATTCAGCGGGCAATAGATCAAGCCTGCCGGGCACACTGCTGA CATCAAGCTCAAACATTTGGAATGACGGCGCAGCAGTTGATGCCCATGCG TACACAGCCAAGGTGTACGACTACTATAAGAACAAGTTTGGCAGAAATAG CATCGACGGAAATGGATTTCAACTTAAATCAACGGTGCACTACTCATCAA GATATAACAATGCGTTTTGGAACGGAGTGCAGATGGTCTACGGAGACGGC GACGGCGTGACATTTATTCCGTTTAGCGCCGACCCGGACGTGATTGGACA TGAACTGACACATGGAGTGACAGAGCATACGGCGGGACTGGAATATTACG GCGAAAGCGGCGCACTGAACGAAAGCATCTCAGACATTATTGGAAACGCA ATCGATGGCAAAAACTGGCTGATTGGCGATCTGATTTATACGCCGAATAC ACCGGGCGATGCACTGAGATCAATGGAGAATCCGAAGCTGTACAACCAAC CGGACAGATACCAAGATAGATACACAGGACCGTCAGACAACGGCGGAGTC CATATCAACAGCGGAATCAATAACAAAGCCTTTTACCTGATCGCCCAAGG CGGAACGCACTATGGCGTTACAGTCAATGGCATCGGAAGAGATGCCGCAG TTCAGATTTTCTATGACGCGCTGATCAACTATCTGACGCCTACAAGCAAT TTCTCAGCAATGAGAGCCGCAGCAATCCAAGCAGCCACGGATCTGTATGG AGCCAATTCATCACAAGTTAATGCTGTTAAGAAGGCTTATACGGCAGTGG GAGTTAACTAA
[0385] The amino acid sequence of the PehPro1 precursor protein expressed from plasmid pGX148(AprE-PehPro1) is depicted in SEQ ID NO: 20. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text.
TABLE-US-00034 MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKAPQDQAAPFGGFTPQLIT GESWSAPQGVSGEEKIWKYLESKQESFQIGQTVDLKKQLKIIGQTTDEKT GTTHYRLQQYVGGVPVYGGVQTIHVNKEGQVTSLIGSLLPDQQQQVSKSL NSQISEAQAIAVAQKDTEAAVGKLGEPQKTPEADLYVYLHNGQPVLAYVT EVNVLEPEAIRTRYFISAEDGSILFKYDILAHATGTGKGVLGDTKSFTTT QSGSTYQLKDTTRGQGIVTYSAGNRSSLPGTLLTSSSNIWNDGAAVDAHA YTAKVYDYYKNKFGRNSIDGNGFQLKSTVHYSSRYNNAFWNGVQMVYGDG DGVTFIPFSADPDVIGHELTHGVTEHTAGLEYYGESGALNESISDIIGNA IDGKNWLIGDLIYTPNTPGDALRSMENPKLYNQPDRYQDRYTGPSDNGGV HINSGINNKAFYLIAQGGTHYGVTVNGIGRDAAVQIFYDALINYLTPTSN FSAMRAAAIQAATDLYGANSSQVNAVKKAYTAVGVN.
Example 4.3
Proteolytic Activity of Metalloprotease PehPro1
[0386] The proteolytic activity of purified metalloprotease PehPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat#74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H2O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A440) using a SpectraMax 190. Net A440 was calculated by subtracting the A440 of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays. The proteolytic activity is shown as Net A440. The proteolytic assay with azo-casein as the substrate (shown in FIG. 4.2) indicates that PehPro1 is an active protease.
Example 4.4
pH Profile of Metalloprotease PehPro1
[0387] With azo-casein as the substrate, the pH profile of metalloprotease PehPro1 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl Milli-Q H2O diluted enzyme (250 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 4.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in FIG. 4.3, the optimal pH of PehPro1 is 7, with greater than 70% of maximal activity retained between 5.5 and 9.5.
Example 4.5
Temperature Profile of Metalloprotease PehPro1
[0388] The temperature profile of metalloprotease PehPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 4.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H2O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20˜90° C.) for 5 min. After the incubation, 5 μl of diluted enzyme (100 ppm) or H2O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 4.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in FIG. 4.4 suggest that PehPro1 showed an optimal temperature at 70° C., and retained greater than 70% of its maximum activity between 60 and 75° C.
Example 4.6
Cleaning Performance of Metalloprotease PehPro1
[0389] The cleaning performance of PehPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca2+:Mg2+=3:1) (detergent composition shown in Table 4.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the "Initial performance") using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the "Wash-off"). The sum of two absorbance measurements ("Initial performance" plus "Wash-off") gives the "Total performance", which measures the protease activity on the model stain; and Net A405 was subsequently calculated by subtracting the A405 of the "Total performance" of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 for PehPro1 in AT detergent is shown in FIGS. 4.5A and 4.5B.
TABLE-US-00035 TABLE 4.1 Composition of AT dish detergent Concentration Ingredient (mg/ml) MGDA (methylglycinediacetic acid) 0.143 Sodium citrate 1.86 Citric acid* varies Plurafac ® LF 18B (a non-ionic surfactant) 0.029 Bismuthcitrate 0.006 Bayhibit ® S (Phosphonobutantricarboxylic 0.006 acid sodium salt) Acusol ® 587 (a calcium polyphosphate 0.029 inhibitor) PEG 6000 0.043 PEG 1500 0.1 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid.
Example 4.7
Comparison of PehPro1 to Other Proteases
A. Identification of Homologous Proteases
[0390] Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The mature protein amino acid sequence for PehPro1 (SEQ ID NO: 18) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 4.2A and 4.2B provide a list of sequences with the percent identity to PehPro1. The length in Table 4.2 refers to the entire sequence length of the homologous proteases.
TABLE-US-00036 TABLE 4.2A List of sequences with percent identity to PehPro1 protein identified from the NCBI non-redundant protein database PID to Accession # PehPro1 Organism Length ZP_09077634.1 88 Paenibacillus elgii B69 524 ZP_09071078.1 74 Paenibacillus larvae subsp. 529 larvae B-3650 YP_003872179.1 74 Paenibacillus polymyxa E681 592 P29148 73 Paenibacillus polymyxa 590 P43263 68 Brevibacillus brevis 527 ZP_09775365.1 68 Paenibacillus sp. Aloe-11 580 ZP_10241029.1 67 Paenibacillus peoriae KCTC 599 3763 ZP_10575942.1 66 Brevibacillus sp. BC25 528 YP_002770810.1 67 Brevibacillus brevis NBRC 528 100599 ZP_08640523.1 64 Brevibacillus laterosporus 564 LMG 15441 YP_004646155.1 63 Paenibacillus mucilaginosus 525 KNP414 ZP_08093424.1 60 Planococcus donghaensis 553 MPA1U2 YP_003670279.1 59 Geobacillus sp. C56-T3 546 P00800 59 Bacillus thermoproteolyticus 548
TABLE-US-00037 TABLE 4.2B List of sequences with percent identity to PehPro1 protein identified from the Genome Quest Patent database PID to Patent ID # PehPro1 Organism Length JP2005229807-0019 74.5 Paenibacillus polymyxa 566 US20120107907-0187 74.09 Bacillus polymyxa 302 US8114656-0186 68.21 Bacillus brevis 304 WO2004011619-0044 63.25 empty 507 EP2390321-0185 62.9 Bacillus cereus 317 WO2012110563-0004 62.7 Bacillus megaterium 320 WO2012110563-0005 62.58 Bacillus cereus 320 JP1995184649-0001 62.5 Lactobacillus sp. 566 JP2005333991-0002 62.38 empty 562 EP2178896-0184 62.18 Bacillus anthracis 566 JP1994014788-0003 61.94 empty 317 EP2390321-0178 61.86 Bacillus thuringiensis 566 US6518054-0002 60.84 Bacillus sp. 316 US8114656-0176 60.13 Bacillus stearothermophilus 548 US6103512-0003 59.81 empty 319 US20120107907-0184 59.49 Bacillus caldoyticus 319
B. Alignment of Homologous Protease Sequences
[0391] The amino acid sequence of predicted mature PehPro1 (SEQ ID NO: 18) was aligned with thermolysin (P00800, Bacillus thermoproteolyticus) and protease from Paenibacillus elgii B69 (ZP_09077634.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters. FIG. 4.6 shows the alignment of PehPro1 with these protease sequences.
C. Phylogenetic Tree
[0392] A phylogenetic tree for precursor protein PehPro1 (SEQ ID NO: 17) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. Mol Biol. Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in FIG. 4.7.
Example 5.1
Cloning of Paenibacillus barcinonensis Metalloprotease PbaPro1
[0393] A strain (DSM15478) of Paenibacillus barcinonensis was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the Paenibacillus barcinonensis strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina's next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in Paenibacillus barcinonensis encodes a metalloprotease and the sequence of this gene, called PbaPro1, is provided in SEQ ID NO: 21. The corresponding protein encoded by the PbaPro1 gene is shown in SEQ ID NO: 22. At the N-terminus, the protein has a signal peptide with a length of 25 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PbaPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the Paenibacillus polymyxa Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PbaPro1 protein is shown in SEQ ID NO: 23.
[0394] The nucleotide sequence of the PbaPro1 gene isolated from Paenibacillus barcinonensis is set forth as SEQ ID NO: 21. The sequence encoding the predicted native signal peptide is shown in italics:
TABLE-US-00038 ATGAAATTGACCAAAATTATGCCAACAATTCTTGCAGGAGCTCTTTTGCT CACATCCCTGTCCTCTGCAGCAGCAATGCCGTTATCTGACTCATCCATTC CATTTGAGGGCCCCTACACCTCCGAGGAGAGTATTCTGTTGAACAACAAC CCGGACGAAATGATTTATAATTTTCTTGCACAACAAGAGCAATTTCTGAA TGCCGACGTCAAAGGACAGCTCAAAATCATTAAACGCAACACAGACACTT CCGGCATCAGACACTTTCGTCTGAAGCAATACATCAAAGGTGTTCCGGTT TACGGCGCAGAACAAACGATCCATCTGGACAAGAACGGAGCTGTAACTTC CGCACTCGGCGATCTTCCGCCAATTGAAGAACAGGCTGTTCCGAATGATG GCGTTCCCGCAATCAGTGCAGACGATGCCATCCGTGCCGCCGAGAATGAA GCCACCTCCCGTCTTGGAGAGCTTGGCGCACCAGAGCTTGAGCCAAAGGC CGAATTAAACATTTATCATCATGAAGATGACGGACAAACCTACCTCGTTT ACATTACGGAAGTTAACGTGCTTGAGCCTTCCCCGCTACGGACCAAATAT TTTATTAACGCCCTTGATGGAAGCATCGTATCTCAATACGATATTATCAA CTTTGCCACAGGCACCGGTACAGGCGTGCATGGTGATACCAAAACACTGA CGACAACTCAATCCGGCAGCACCTATCAGCTGAAAGATACAACTCGTGGA AAAGGCATTCAAACCTATACTGCGAACAATCGCTCCTCGCTTCCAGGCAG CTTGTCTACCAGTTCCAATAACGTATGGACAGACCGTGCAGCTGTAGATG CGCACGCCTATGCTGCCGCCACATATGACTTCTACAAAAACAAATTCAAT CGCAACGGCATTGACGGAAACGGGCTGTTGATTCGCTCTACAGTGCATTA TGGCTCCAACTATAAAAACGCCTTCTGGAACGGAGCACAGATTGTCTATG GAGATGGCGATGGCATCGAGTTCGGTCCCTTCTCCGGTGATCTCGATGTT GTCGGACATGAATTGACACACGGGGTGATTGAATATACAGCCAATCTCGA ATATCGCAATGAGCCGGGTGCTTTAAACGAAGCTTTTGCCGACATTATGG GGAACACCATCGAAAGCAAAAACTGGCTGCTTGGCGACGGAATCTATACT CCAAACATTCCAGGTGATGCCCTGCGCTCGTTATCCGACCCTACGCTGTA TAACCAGCCTGACAAATACAGTGATCGCTACACTGGCTCTCAGGATAATG GCGGTGTGCATATCAACAGCGGGATCATTAACAAAGCATATTATCTTGCA GCCCAAGGCGGTACTCATAACGGGGTAACCGTTAGCGGCATCGGCCGGGA TAAAGCAGTACGTATTTTCTATAGCACGCTGGTGAACTACCTGACGCCAA CCTCCAAATTTGCAGCAGCCAAAACAGCGACAATTCAGGCAGCCAAGGAC CTGTACGGTGCCAATTCCGCTGAAGCTACGGCAATCACCAAAGCTTATCA AGCGGTAGGTTTG
[0395] The amino acid sequence of the PbaPro1 precursor protein is set forth as SEQ ID NO: 22. The predicted signal sequence is shown in italics, and the predicted pro-peptide is shown in underlined text:
TABLE-US-00039 MKLTKIMPTILAGALLLTSLSSAAAMPLSDSSIPFEGPYTSEESILLNNN PDEMIYNFLAQQEQFLNADVKGQLKIIKRNTDTSGIRHFRLKQYIKGVPV YGAEQTIHLDKNGAVTSALGDLPPIEEQAVPNDGVPAISADDAIRAAENE ATSRLGELGAPELEPKAELNIYHHEDDGQTYLVYITEVNVLEPSPLRTKY FINALDGSIVSQYDIINFATGTGTGVHGDTKTLTTTQSGSTYQLKDTTRG KGIQTYTANNRSSLPGSLSTSSNNVWTDRAAVDAHAYAAATYDFYKNKFN RNGIDGNGLLIRSTVHYGSNYKNAFWNGAQIVYGDGDGIEFGPFSGDLDV VGHELTHGVIEYTANLEYRNEPGALNEAFADIMGNTIESKNWLLGDGIYT PNIPGDALRSLSDPTLYNQPDKYSDRYTGSQDNGGVHINSGIINKAYYLA AQGGTHNGVTVSGIGRDKAVRIFYSTLVNYLTPTSKFAAAKTATIQAAKD LYGANSAEATAITKAYQAVGL
[0396] The amino acid sequence of the predicted mature form of PbaPro1 is set forth as SEQ ID NO: 23:
TABLE-US-00040 ATGTGTGVHGDTKTLTTTQSGSTYQLKDTTRGKGIQTYTANNRSSLPGSL STSSNNVWTDRAAVDAHAYAAATYDFYKNKFNRNGIDGNGLLIRSTVHYG SNYKNAFWNGAQIVYGDGDGIEFGPFSGDLDVVGHELTHGVIEYTANLEY RNEPGALNEAFADIMGNTIESKNWLLGDGIYTPNIPGDALRSLSDPTLYN QPDKYSDRYTGSQDNGGVHINSGIINKAYYLAAQGGTHNGVTVSGIGRDK AVRIFYSTLVNYLTPTSKFAAAKTATIQAAKDLYGANSAEATAITKAYQA VGL
Example 5.2
Expression of Paenibacillus barcinonensis Metalloprotease PbaPro1
[0397] The DNA sequence of the propeptide-mature form of PbaPro1 was synthesized and inserted into the Bacillus subtilis expression vector p2JM103BBI (Vogtentanz, Protein Expr Purif, 55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX147(AprE-PbaPro1) (FIG. 5.1). Ligation of this gene encoding the PbaPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3' end of the B. subtilis AprE signal sequence and the 5' end of the predicted PbaPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in FIG. 1 was labeled pGX147(AprE-PbaPro1). As shown in FIG. 5.1, pGX147(AprE-PbaPro1) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in B. subtilis, and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PbaPro1 (SEQ ID NO: 24). The translation product of the synthetic AprE-PbaPro1 gene is shown in SEQ ID NO: 25.
[0398] The pGX147(AprE-PbaPro1) plasmid was then transformed into B. subtilis cells (degU.sup.Hy32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat#232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl2).
[0399] The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl2 and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above; and the active flow-through fractions were collected and concentrated. The sample was loaded onto a 320 ml Superdex 75 gel filtration column pre-equilibrated with the loading buffer described above containing 0.15 M NaCl. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses.
[0400] The nucleotide sequence of the synthesized PbaPro1 gene in plasmid pGX147(AprE-PbaPro1) is depicted in SEQ ID NO: 24. The sequence encoding the three residue addition (AGK) is shown in bold:
TABLE-US-00041 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAAT CTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCTGCTGGAAAAATGC CTCTGTCAGACAGCAGCATTCCGTTTGAGGGCCCGTACACATCAGAAGAA AGCATCCTGCTGAACAACAACCCGGACGAGATGATCTACAATTTCCTGGC ACAGCAGGAGCAGTTCCTGAACGCAGACGTGAAGGGCCAGCTGAAAATCA TCAAAAGAAACACAGACACGAGCGGCATCAGACACTTCAGACTGAAGCAG TACATCAAGGGCGTCCCGGTTTACGGCGCTGAGCAGACAATCCACCTGGA CAAAAATGGCGCAGTGACGAGCGCACTTGGAGATCTGCCGCCGATTGAAG AGCAAGCAGTCCCGAACGATGGCGTTCCGGCGATTAGCGCTGATGACGCT ATCAGAGCCGCGGAAAACGAAGCGACGTCAAGACTGGGAGAACTTGGCGC ACCGGAACTTGAACCGAAGGCGGAACTGAACATCTATCACCACGAAGACG ATGGACAGACGTACCTGGTGTACATCACGGAGGTGAATGTGCTGGAGCCG TCACCGCTGAGAACAAAATACTTCATCAATGCGCTGGATGGCAGCATCGT TAGCCAATACGACATCATTAACTTCGCCACAGGCACGGGCACAGGCGTTC ATGGCGACACAAAAACGCTTACGACAACACAGTCAGGCTCAACGTACCAG CTGAAAGACACAACAAGAGGCAAGGGCATCCAGACGTATACAGCCAATAA CAGAAGCTCACTTCCGGGCTCACTGTCAACAAGCAGCAATAATGTCTGGA CGGACAGAGCTGCAGTGGACGCGCACGCGTATGCTGCGGCCACGTACGAC TTCTACAAGAACAAGTTCAACAGAAACGGCATTGATGGCAACGGCCTGCT TATTAGAAGCACGGTCCACTACGGCTCAAACTACAAGAATGCGTTTTGGA ACGGCGCCCAAATTGTTTATGGCGATGGAGACGGCATCGAGTTCGGACCT TTTAGCGGCGACCTGGATGTGGTCGGACATGAACTGACGCACGGCGTTAT CGAGTATACGGCGAATCTGGAATACAGAAATGAACCGGGCGCTCTGAATG AGGCCTTCGCGGATATCATGGGCAACACAATTGAGAGCAAAAACTGGCTT CTGGGCGACGGAATCTACACGCCGAACATTCCGGGAGATGCACTGAGATC ACTGAGCGACCCTACGCTGTACAACCAGCCGGACAAATACAGCGACAGAT ACACGGGATCACAGGACAATGGCGGCGTCCATATTAACTCAGGCATCATC AACAAAGCGTATTATCTGGCAGCTCAAGGCGGCACGCATAATGGCGTCAC AGTTAGCGGAATCGGCAGAGACAAGGCCGTCAGAATTTTCTACTCAACGC TGGTGAACTACCTGACACCGACAAGCAAGTTTGCAGCCGCCAAAACAGCC ACGATTCAGGCAGCAAAGGACCTGTACGGAGCGAACTCAGCAGAGGCCAC AGCGATTACGAAGGCTTATCAAGCCGTGGGACTGTAA
[0401] The amino acid sequence of the PbaPro1 precursor protein expressed from plasmid pGX147(AprE-PbaPro1) is depicted in SEQ ID NO: 25. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text.
TABLE-US-00042 MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKMPLSDSSIPFEGPYTSEE SILLNNNPDEMIYNFLAQQEQFLNADVKGQLKIIKRNTDTSGIRHFRLKQ YIKGVPVYGAEQTIHLDKNGAVTSALGDLPPIEEQAVPNDGVPAISADDA IRAAENEATSRLGELGAPELEPKAELNIYHHEDDGQTYLVYITEVNVLEP SPLRTKYFINALDGSIVSQYDIINFATGTGTGVHGDTKTLTTTQSGSTYQ LKDTTRGKGIQTYTANNRSSLPGSLSTSSNNVWTDRAAVDAHAYAAATYD FYKNKFNRNGIDGNGLLIRSTVHYGSNYKNAFWNGAQIVYGDGDGIEFGP FSGDLDVVGHELTHGVIEYTANLEYRNEPGALNEAFADIMGNTIESKNWL LGDGIYTPNIPGDALRSLSDPTLYNQPDKYSDRYTGSQDNGGVHINSGII NKAYYLAAQGGTHNGVTVSGIGRDKAVRIFYSTLVNYLTPTSKFAAAKTA TIQAAKDLYGANSAEATAITKAYQAVGL
Example 5.3
Proteolytic Activity of Metalloprotease PbaPro1
[0402] The proteolytic activity of purified metalloprotease PbaPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat#74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H2O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A440) using a SpectraMax 190. Net A440 was calculated by subtracting the A440 of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays.
[0403] The proteolytic activities are shown as Net A440. The proteolytic assay with azo-casein as the substrate (shown in FIG. 5.2) indicates that PbaPro1 is an active protease.
Example 5.4
pH Profile of Metalloprotease PbaPro1
[0404] With azo-casein as the substrate, the pH profile of metalloprotease PbaPro1 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 5 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl Milli-Q H2O diluted enzyme (125 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 5.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in FIG. 5.3, the optimal pH of PbaPro1 is 8, with greater than 70% of maximal activity retained between 7 and 9.
Example 5.5
Temperature Profile of Metalloprotease PbaPro1
[0405] The temperature profiles of metalloprotease PbaPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 5.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H2O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20˜90° C.) for 5 min After the incubation, 5 μl of diluted enzyme (50 ppm) or H2O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 5.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in FIG. 5.4 suggest that PbaPro1 showed an optimal temperature at 50° C., and retained greater than 70% of its maximum activity between 45 and 55° C.
Example 5.6
Cleaning Performance of Metalloprotease PbaPro1
[0406] The cleaning performance of PbaPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca2+:Mg2+=3:1) (detergent composition shown in Table 5.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the "Initial performance") using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the "Wash-off"). The sum of two absorbance measurements ("Initial performance" plus "Wash-off") gives the "Total performance", which measures the protease activity on the model stain; and Net A405 was subsequently calculated by subtracting the A405 of the "Total performance" of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 in AT detergent for PbaPro1 is shown in FIGS. 5.5A and 5.5B.
TABLE-US-00043 TABLE 5.1 Composition of AT dish detergent formula with bleach Concentration Ingredient (mg/ml) MGDA (methylglycinediacetic acid) 0.143 Sodium citrate 1.86 Citric acid* varies PAP (peracid N,N-phthaloylaminoperoxycaproic acid) 0.057 Plurafac ® LF 18B (a non-ionic surfactant) 0.029 Bismuthcitrate 0.006 Bayhibit ® S (Phosphonobutantricarboxylic 0.006 acid sodium salt) Acusol ® 587 (a calcium polyphosphate inhibitor) 0.029 PEG 6000 0.043 PEG 1500 0.1 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid.
Example 5.7
Comparison of PbaPro1 to Other Proteases
[0407] A. Identification of Homologous Proteases
[0408] Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PbaPro1 (SEQ ID NO: 23) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 5.2A and 5.2B provide a list of sequences with the percent identity to PbaPro1. The length in Table 5.2 refers to the entire sequence length of the homologous proteases.
TABLE-US-00044 TABLE 5.2A List of sequences with percent identity to PbaPro1 protein identified from the NCBI non-redundant protein database PID to Accession # PbaPro1 Organism Length AAB02774.1 56 Geobacillus stearothermophilus 552 P00800 56 Bacillus stermoproteolyticus 548 AAA22623.1 57 Bacillus caldolyticus 544 YP_003670279.1 57 Geobacillus sp. C56-T3 546 AAC43402.1 57 Alicyclobacillus acidocaldarius 546 YP_003597483.1 57 Bacillus megaterium DSM 319 562 ZP_08093424.1 57 Planococcus donghaensis 553 MPA1U2 ZP_08640523.1 59 Brevibacillus laterosporus 564 LMG 15441 ZP_04216147.1 59 Bacillus cereus Rock3-44 566 YP_001373863.1 60 Bacillus cytotoxicus NVH 565 391-98 YP_004646155.1 60 Paenibacillus mucilaginosus 525 KNP414 ZP_10738945.1 61 Brevibacillus sp. CF112 528 CAA43589.1 63 Brevibacillus brevis 527 ZP_02326602.1 64 Paenibacillus larvae subsp. 520 larvae BRL-230010 ZP_02326503.1 65 Paenibacillus larvae subsp. 520 larvae B-3650 ZP_09077634.1 66 Paenibacillus elgii B69 524 ZP_08511445.1 68 [Paenibacillus sp. HGF7 525 ZP_09775364.1 70 Paenibacillus sp. Aloe-11 593 YP_005073223.1 70 Paenibacillus terrae HPL-003 591 ZP_10241030.1 70 Paenibacillus peoriae KCTC 593 3763 YP_003948511.1| 71 Paenibacillus polymyxa SC2 592
TABLE-US-00045 TABLE 5.2B List of sequences with percent identity to PbaPro1 protein identified from the Genome Quest Patent database PID to Patent # PbaPro1 Organism Length JP2005333991-0002 56.91 562 WO2012110562-0007 56.96 Bacillus cereus 320 WO2012110562-0006 57.23 Bacillus megaterium 320 EP2390321-0178 57.23 Bacillus thuringiensis 566 EP2390321-0184 57.56 Bacillus caldoyticus 319 WO2007044993-0184 57.56 Bacillus sp. 319 US20120107907-0177 57.56 Bacillus caldolyticus 544 CN102168095-0002 57.88 319 WO2012110562-0004 57.88 Bacillus caldolyticus 319 WO2012110562-0003 57.88 Geobacillus 319 stearothermophilus WO2004011619-0056 57.88 546 JP1995184649-0001 57.88 Lactobacillus sp. 566 JP2010535248-0240 57.88 Bacillus anthracis 566 US6518054-0001 58.2 Bacillus sp. 319 US6103512-0003 58.2 319 WO2011163237-0001 58.2 Geobacillus 548 stearothermophilus JP1994014788-0003 58.25 317 US8114656-0185 58.9 Bacillus cereus 317 US20120107907-0179 58.9 Bacillus cereus 566 WO2012110563-0005 59.22 Bacillus cereus 320 WO2004011619-0044 59.6 507 US20120107907-0186 63.25 Bacillus brevis 304 JP2005229807-0018 70.86 Paenibacillus polymyxa 566 EP2390321-0187 71.1 Bacillus polymyxa 302 JP2009511072-0203 71.1 Paenibacillus polymyxa 302
B. Alignment of Homologous Protease Sequences
[0409] The amino acid sequence of the predicted mature PbaPro1 (SEQ ID NO: 23) was aligned with Thermolysin (P00800, Bacillus thermoproteolyticus), and protease from Paenibacillus polymyxa SC2 (YP_003948511.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters. FIG. 5.6 shows the alignment of PbaPro1 with these protease sequences.
C. Phylogenetic Tree
[0410] A phylogenetic tree for full length sequence of PbaPro1 (SEQ ID NO: 22) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. Mol Biol. Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in FIG. 5.7.
Example 6.1
Cloning of Paenibacillus polymyxa SC2 Metalloprotease PpoPro1
[0411] The nucleic acid sequence for the PpoPro1 gene was identified in the NCBI database (NCBI Reference Sequence: NC_014622.1 from 4536397-4538175) and is provided in SEQ ID NO: 26. The corresponding protein encoded by the PpoPro1 gene is shown in SEQ ID NO: 27. At the N-terminus, the protein has a signal peptide with a length of 24 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PpoPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the Paenibacillus polymyxa Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PpoPro1 protein is shown in SEQ ID NO: 28.
[0412] The nucleotide sequence of the PpoPro1 gene identified from NCBI database is set forth as SEQ ID NO: 26. The sequence encoding the predicted native signal peptide is shown in italics:
TABLE-US-00046 ATGAAAAAAGTATGGGTTTCGCTTCTTGGAGGAGCTATGTTATTAGGGTC TGTCGCGTCTGGTGCATCTGCGGAGAGTTCCGTTTCGGGGCCAGCTCAGC TTACACCGACCTTCCACGCCGAGCAATGGAAAGCACCTACCTCGGTATCG GGGGATGACATTGTATGGAGCTATTTAAATCGACAAAAGAAATCGTTGCT GGGTGTGGATAGCTCCAGTGTACGTGAACAATTCCGAATCGTTGATCGCA CAAGCGACAAATCCGGTGTAAGCCATTATCGACTGAAGCAGTATGTAAAC GGAATTCCCGTGTATGGAGCTGAACAAACTATTCATGTGGGCAAATCTGG TGAGGTCACCTCTTACTTAGGAGCGGTGGTTAATGAGGATCAGCAGGCAG AAGCTACGCAAGGTACAACTCCAAAAATCAGCGCTTCTGAAGCGGTCTAC ACCGCATATAAAGAAGCAGCTGCACGGATTGAAGCCCTCCCTACCTCCGA CGATACTATTTCTAAAGACGCTGAGGAGCCAAGCAGTGTAAGTAAAGATA CTTACGCCGAAGCAGCTAACAACGAAAAAACGCTTTCTGTTGATAAGGAC GAGCTGAGTCTTGATCAGGCATCTGTCCTGAAAGATAGCAAAATTGAAGC AGTGGAACCAGAAAAAAGTTCCATTGCCAAAATCGCTAATCTGCAGCCTG AAGTAGATCCTAAAGCAGAACTCTACTACTACCCTAAGGGGGATGACCTG CTGCTGGTTTATGTAACAGAAGTTAATGTTTTAGAACCTGCCCCACTGCG TACCCGCTACATTATTGATGCCAATGACGGCAGCATCGTATTCCAGTATG ACATCATTAATGAAGCGACAGGCACAGGTAAAGGTGTGCTTGGTGATTCC AAATCGTTCACTACTACCGCTTCCGGCAGTAGCTACCAGTTAAAAGATAC AACACGCGGTAACGGAATCGTGACTTACACGGCCTCCAACCGTCAAAGCA TCCCAGGTACCATTTTGACAGATGCCGATAATGTATGGAATGATCCAGCT GGTGTGGACGCCCATGCGTATGCTGCTAAAACCTATGATTACTATAAAGC CAAATTTGGACGCAACAGCATTGACGGACGCGGTCTGCAACTTCGTTCGA CGGTCCATTACGGTAGTCGCTACAACAATGCCTTCTGGAACGGCTCCCAA ATGACTTATGGAGATGGAGATGGTAGCACATTTATCGCCTTCAGCGGGGA CCCCGATGTAGTAGGACATGAACTTACGCATGGTGTCACAGAGTATACTT CGAATTTGGAATATTACGGAGAGTCCGGCGCATTGAATGAAGCTTTCTCA GACGTTATCGGGAATGACATTCAGCGCAAAAACTGGCTTGTAGGCGATGA TATTTACACGCCAAACATTGCAGGCGATGCCCTTCGCTCAATGTCCAATC CAACCCTGTACGATCAACCAGATCACTATTCCAACCTGTACAGAGGCAGC TCCGATAACGGCGGTGTTCACACCAACAGCGGTATTATCAATAAAGCTTA CTACTTGTTAGCACAAGGTGGTAATTTCCATGGCGTAACTGTAAATGGAA TTGGCCGTGATGCAGCGGTGCAAATTTACTACAGTGCCTTTACGAACTAC CTGACTTCTTCTTCCGACTTCTCCAACGCACGTGCTGCTGTGATCCAAGC CGCAAAAGATCTGTACGGGGCGAACTCAGCAGAAGCAACTGCAGCTGCCA AGTCTTTTGACGCTGTAGGCGTAAACTAA
[0413] The amino acid sequence of the PpoPro1 precursor protein is set forth as SEQ ID NO: 27. The predicted signal sequence is shown in italics, and the predicted propeptide is shown in underlined text:
TABLE-US-00047 MKKVWVSLLGGAMLLGSVASGASAESSVSGPAQLTPTFHAEQWKAPTSVS GDDIVWSYLNRQKKSLLGVDSSSVREQFRIVDRTSDKSGVSHYRLKQYVN GIPVYGAEQTIHVGKSGEVTSYLGAVVNEDQQAEATQGTTPKISASEAVY TAYKEAAARIEALPTSDDTISKDAEEPSSVSKDTYAEAANNEKTLSVDKD ELSLDQASVLKDSKIEAVEPEKSSIAKIANLQPEVDPKAELYYYPKGDDL LLVYVTEVNVLEPAPLRTRYIIDANDGSIVFQYDIINEATGTGKGVLGDS KSFTTTASGSSYQLKDTTRGNGIVTYTASNRQSIPGTILTDADNVWNDPA GVDAHAYAAKTYDYYKAKFGRNSIDGRGLQLRSTVHYGSRYNNAFWNGSQ MTYGDGDGSTFIAFSGDPDVVGHELTHGVTEYTSNLEYYGESGALNEAFS DVIGNDIQRKNWLVGDDIYTPNIAGDALRSMSNPTLYDQPDHYSNLYRGS SDNGGVHTNSGIINKAYYLLAQGGNFHGVTVNGIGRDAAVQIYYSAFTNY LTSSSDFSNARAAVIQAAKDLYGANSAEATAAAKSFDAVGVN
[0414] The amino acid sequence of the predicted mature form of PpoPro1 is set forth as SEQ ID NO: 28:
TABLE-US-00048 ATGTGKGVLGDSKSFTTTASGSSYQLKDTTRGNGIVTYTASNRQSIPGTI LTDADNVWNDPAGVDAHAYAAKTYDYYKAKFGRNSIDGRGLQLRSTVHYG SRYNNAFWNGSQMTYGDGDGSTFIAFSGDPDVVGHELTHGVTEYTSNLEY YGESGALNEAFSDVIGNDIQRKNWLVGDDIYTPNIAGDALRSMSNPTLYD QPDHYSNLYRGSSDNGGVHTNSGIINKAYYLLAQGGNFHGVTVNGIGRDA AVQIYYSAFTNYLTSSSDFSNARAAVIQAAKDLYGANSAEATAAAKSFDA VGVN
Example 6.2
Expression of Paenibacillus polymyxa SC2 Metalloprotease PpoPro1
[0415] The DNA sequence of the propeptide-mature form of PpoPro1 was synthesized and inserted into the Bacillus subtilis expression vector p2JM103BBI (Vogtentanz, Protein Expr Purif, 55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX138(AprE-PpoPro1) (FIG. 1). Ligation of this gene encoding the PpoPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3' end of the B. subtilis AprE signal sequence and the 5' end of the predicted PpoPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in FIG. 6.1, labeled pGX138(AprE-PpoPro1 contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in B. subtilis, and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PpoPro1 (SEQ ID NO: 29). The translation product of the synthetic AprE-PpoPro1 gene is shown in SEQ ID NO: 30.
[0416] The pGX138(AprE-PpoPro1) plasmid was then transformed into B. subtilis cells (degU.sup.Hy32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat#232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl2).
[0417] The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl2 and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above, PpoPro1 was eluted from the column with a linear salt gradient from 0 to 0.25 M NaCl in the loading buffer. The corresponding active fractions were collected and concentrated. The sample was loaded onto a 320 ml Superdex 75 gel filtration column pre-equilibrated with the loading buffer described above containing 0.15 M NaCl. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses.
[0418] The nucleotide sequence of the synthesized PpoPro1 gene in plasmid pGX138(AprE-PpoPro1) is depicted in SEQ ID NO: 29. The sequence encoding the three residue addition (AGK) is shown in bold:
TABLE-US-00049 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAAT CTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCTGCTGGAAAAGAAT CATCAGTGTCAGGACCGGCTCAGCTTACACCGACATTTCACGCAGAACAA TGGAAGGCTCCGACGTCAGTTTCAGGAGACGACATCGTGTGGAGCTACCT GAATAGACAGAAGAAAAGCCTGCTGGGAGTGGATAGCAGCAGCGTCAGAG AGCAGTTCAGAATCGTTGACAGAACGAGCGACAAAAGCGGAGTCAGCCAT TATAGACTGAAGCAGTACGTGAATGGCATCCCGGTTTATGGCGCAGAGCA GACAATTCATGTTGGCAAGAGCGGAGAAGTCACAAGCTATCTGGGCGCTG TGGTCAATGAAGATCAACAAGCCGAGGCTACACAGGGAACAACGCCGAAA ATTAGCGCCTCAGAGGCAGTCTACACGGCGTACAAAGAAGCGGCTGCAAG AATCGAAGCCCTGCCGACATCAGACGATACAATTTCAAAAGATGCGGAGG AGCCGAGCTCAGTTAGCAAGGATACATACGCGGAAGCCGCAAACAATGAG AAAACACTGAGCGTGGACAAGGACGAGCTGTCACTTGATCAGGCTAGCGT CCTTAAAGACAGCAAGATCGAGGCCGTTGAGCCTGAAAAGTCATCAATTG CGAAAATCGCCAATCTGCAACCTGAAGTCGACCCGAAGGCGGAACTGTAC TACTACCCGAAAGGCGATGACCTGCTTCTGGTGTACGTCACGGAAGTGAA CGTCCTGGAACCGGCACCGCTGAGAACAAGATACATCATCGACGCGAACG ACGGAAGCATCGTCTTCCAGTATGACATTATCAACGAAGCAACGGGAACG GGCAAAGGCGTTCTTGGAGACTCAAAGAGCTTCACGACAACGGCTTCAGG AAGCAGCTACCAGCTGAAAGACACGACGAGAGGAAACGGAATCGTCACAT ATACGGCGTCAAACAGACAAAGCATCCCTGGCACAATCCTGACGGATGCT GACAACGTTTGGAATGATCCGGCTGGCGTGGATGCCCATGCTTATGCGGC AAAAACGTATGACTATTACAAGGCGAAGTTCGGCAGAAATTCAATCGATG GCAGAGGACTGCAGCTTAGAAGCACGGTGCACTACGGATCAAGATATAAC AATGCCTTCTGGAACGGCAGCCAGATGACATACGGAGACGGAGATGGAAG CACATTTATTGCATTCAGCGGCGACCCTGATGTGGTTGGCCATGAGCTGA CGCATGGCGTTACAGAATATACGAGCAATCTTGAATACTACGGCGAGTCA GGCGCTCTGAACGAGGCATTTAGCGATGTTATCGGCAATGACATCCAGAG AAAAAACTGGCTGGTGGGCGACGATATTTACACGCCTAATATCGCTGGCG ATGCCCTTAGATCAATGTCAAACCCGACGCTGTATGATCAGCCTGACCAC TACTCAAACCTGTATAGAGGCTCATCAGATAACGGAGGCGTCCATACGAA TAGCGGCATCATTAACAAGGCATATTATCTTCTGGCCCAGGGCGGCAATT TTCATGGAGTGACGGTTAATGGAATTGGAAGAGACGCAGCCGTCCAAATC TACTACAGCGCTTTCACGAACTACCTTACATCAAGCTCAGACTTTAGCAA TGCCAGAGCTGCTGTTATCCAGGCAGCGAAGGATCTTTACGGCGCCAACT CAGCCGAAGCTACGGCCGCAGCTAAATCATTTGATGCAGTGGGCGTTAAT
[0419] The amino acid sequence of the PpoPro1 precursor protein expressed from plasmid pGX138(AprE-PpoPro1) is depicted in SEQ ID NO: 30. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text.
TABLE-US-00050 MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKESSVSGPAQLTPTFHAEQ WKAPTSVSGDDIVWSYLNRQKKSLLGVDSSSVREQFRIVDRTSDKSGVSH YRLKQYVNGIPVYGAEQTIHVGKSGEVTSYLGAVVNEDQQAEATQGTTPK ISASEAVYTAYKEAAARIEALPTSDDTISKDAEEPSSVSKDTYAEAANNE KTLSVDKDELSLDQASVLKDSKIEAVEPEKSSIAKIANLQPEVDPKAELY YYPKGDDLLLVYVTEVNVLEPAPLRTRYIIDANDGSIVFQYDIINEATGT GKGVLGDSKSFTTTASGSSYQLKDTTRGNGIVTYTASNRQSIPGTILTDA DNVWNDPAGVDAHAYAAKTYDYYKAKFGRNSIDGRGLQLRSTVHYGSRYN NAFWNGSQMTYGDGDGSTFIAFSGDPDVVGHELTHGVTEYTSNLEYYGES GALNEAFSDVIGNDIQRKNWLVGDDIYTPNIAGDALRSMSNPTLYDQPDH YSNLYRGSSDNGGVHTNSGIINKAYYLLAQGGNFHGVTVNGIGRDAAVQI YYSAFTNYLTSSSDFSNARAAVIQAAKDLYGANSAEATAAAKSFDAVGVN
Example 6.3
Proteolytic Activity of Metalloprotease PpoPro1
[0420] The proteolytic activity of purified PpoPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat#74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μL of the diluted enzyme (or Milli-Q H2O alone as the blank control) was added to the non-binding 96-well microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μL of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μL of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μL supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A440) using a SpectraMax 190. Net A440 was calculated by subtracting the A440 of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of duplicate assays, and the value varies no more than 5%. The proteolytic activity is shown as Net A440. The proteolytic assay with azo-casein as the substrate (FIG. 6.2) indicates PpoPro1 is an active protease.
Example 4
pH Profile of Metalloprotease PpoPro1
[0421] With azo-casein as the substrate, the pH profile of PpoPro1 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μL of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μL diluted enzyme (250 ppm in Milli-Q H2O) in a 96-MTP placed on ice, followed by the addition of 48 μL of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 6.3. Enzyme activity at each pH was reported as relative activity where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in FIG. 6.3, the optimal pH of PpoPro1 is about 7, with greater than 70% of maximal activity retained between 5.5 and 8.5.
Example 6.5
Temperature Profile of Metalloprotease PpoPro1
[0422] The temperature profile of PpoPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assay. The enzyme sample and azo-casein substrate were prepared as in Example 6.3. Prior to the reaction, 50 μL of 1.5% azo-casein and 45 μl Milli-Q H2O were mixed in a 200 μL PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20˜90° C.) for 5 min. After the incubation, 5 μL of diluted PpoPro1 (100 ppm) or H2O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μL of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 6.3. The activity was reported as relative activity where the activity at the optimal temperature was set to be 100%. The tested temperatures were 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in FIG. 6.4 suggests that PpoPro1 showed an optimal temperature at 50° C., and retained greater than 70% of its maximum activity between 40 and 55° C.
Example 6.6
Cleaning Performance of Metalloprotease PpoPro1
[0423] The cleaning performance of PpoPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 or 8 using a model automatic dishwashing (ADW) detergent (AT detergent). Prior to the reaction, purified PpoPro1 was diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent (composition shown in Table 6.1) with 100 ppm water hardness (Ca2+:Mg2+=3:1), in the presence of a bleach component ((Peracid N,N-phthaloylaminoperoxycaproic acid-PAP). To initiate the reaction, 180 μL of AT detergent buffered at pH 6 or 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μL of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μL of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the "Initial performance") using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μL water. Following the addition of 180 μL of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliter of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as "Wash-off"). The sum of two absorbance measurements ("Initial performance" plus "Wash-off") gives the "Total performance", which measures the protease activity on the model stain; and Net A405 was subsequently calculated by subtracting the A405 of the "Total performance" of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 for PpoPro1 in AT dish detergent, in the presence of bleach, is shown in FIGS. 6.5A and 6.5B.
TABLE-US-00051 TABLE 6.1 Composition of AT dish detergent formula with bleach Concentration Ingredient (mg/ml) MGDA (methylglycinediacetic acid) 0.143 Sodium citrate 1.86 Citric acid* varies PAP (peracid N,N-phthaloylaminoperoxycaproic acid) 0.057 Plurafac ® LF 18B (a non-ionic surfactant) 0.029 Bismuthcitrate 0.006 Bayhibit ® S (Phosphonobutantricarboxylic 0.006 acid sodium salt) Acusol ® 587 (a calcium polyphosphate inhibitor) 0.029 PEG 6000 0.043 PEG 1500 0.1 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid.
Example 6.7
Comparison of PpoPro1 to Other Metalloproteases
[0424] Identification of Homologous Proteases
[0425] Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PpoPro1 (SEQ ID NO: 28) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 6.2A and 6.2B provide a list of sequences with the percent identity to PpoPro1. The length in Table 6.2 refers to the entire sequence length of the homologous proteases.
TABLE-US-00052 TABLE 6.2A List of sequences with percent identity to PpoPro1 protein identified from the NCBI non-redundant protein database PID to Accession # PpoPro1 Organism Length P00800 56 Bacillus thermoproteolyticus 548 ZP_08640523.1 57 Brevibacillus laterosporus LMG 564 15441 AAA22623.1 57 Bacillus caldolyticus 544 ZP_08093424.1 59 Planococcus donghaensis 553 MPA1U2 ZP_10738945.1 60 Brevibacillus sp. CF112 528 CAA43589.1 62 Brevibacillus brevis 527 ZP_02326503.1 62 Paenibacillus larvae subsp. 520 larvae BRL-230010 YP_005495105.1 63 Bacillus megaterium WSH-002 562 YP_001373863.1 64 Bacillus cytotoxicus 565 NVH 391-98 ZP_04310163.1 64 Bacillus cereus BGSC 6E1 581 BAA06144.1 64 Lactobacillus sp.] 566 ZP_08511445.1 65 Paenibacillus sp. HGF7 525 ZP_04216147.1 65 Bacillus cereus Rock3-44 566 ZP_09071078.1 68 Paenibacillus larvae subsp. larvae B-3650 ZP_09077634.1 69 Paenibacillus elgii B69 524 YP_005073224.1 79 Paenibacillus terrae HPL-003 595 ZP_10241029.1 80 Paenibacillus peoriae KCTC 599 3763 YP_005073223.1 93 Paenibacillus terrae HPL-003 591 ZP_10241030.1 95 Paenibacillus peoriae KCTC 593 3763 ZP_09775364.1 95 Paenibacillus sp. Aloe-11 593 YP_003872179.1 97 Paenibacillus polymyxa E681 592 YP_003948511.1 100 Paenibacillus polymyxa SC2 592
TABLE-US-00053 TABLE 6.2B List of sequences with percent identity to PpoPro1 protein identified from the Genome Quest Patent database PID to Patent # PpoPro1 Organism Length US20120107907-0187 97.34 Bacillus polymyxa 302 US5962264-0004 65.48 empty 566 WO2012110563-0005 65.16 Bacillus cereus 320 JP1994070791-0002 64.52 empty 317 WO2012110562-0005 64.19 Bacillus cereus 320 WO2012110563-0004 63.34 Bacillus megaterium 320 JP2002272453-0002 61.98 Bacillus megaterium 562 WO2004011619-0047 61.49 empty 532 EP2390321-0186 62.58 Bacillus brevis 304 US6518054-0002 59.22 Bacillus sp. 316 US6518054-0001 58.52 Bacillus sp. 319 US20120107907-0176 58.52 Bacillus stearothermophilis 548 JP2005229807-0019 93.05 Paenibacillus polymyxa 566 WO2012110562-0003 58.2 Geobacillus 319 stearothermophilus WO2004011619-0044 59.27 empty 507 EP2390321-0185 66.13 Bacillus cereus 317 JP1995184649-0001 65.71 Lactobacillus sp. 566 EP2178896-0184 65.38 Bacillus anthracis 566
[0426] Alignment of Homologous Protease Sequences
[0427] The amino acid sequence of predicted mature PpoPro1 (SEQ ID NO: 28) was aligned with thermolysin (P00800, Bacillus thermoproteolyticus) and protease from Paenibacillus polymyxa SC2 (YP_003948511.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters. FIG. 6.6 shows the alignment of PpoPro1 with these protease sequences.
[0428] Phylogenetic Tree
[0429] A phylogenetic tree for precursor PpoPro1 (SEQ ID NO: 27) was built using sequences of representative homologs from Tables 6.2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. Mol Biol. Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in FIG. 6.7.
Example 7.1
Cloning of Paenibacillus Hunanensis Metalloprotease PhuPro1
[0430] A strain (DSM22170) of Paenibacillus hunanensis was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the Paenibacillus hunanensis strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina's next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in Paenibacillus hunanensis encodes a metalloprotease and the sequence of this gene, called PhuPro1, is provided in SEQ ID NO: 31. This gene has an alternative start codon (TTG). The corresponding protein encoded by the PhuPro1 gene is shown in SEQ ID NO: 32. At the N-terminus, the protein has a signal peptide with a length of 23 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PhuPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the Paenibacillus polymyxa Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PhuPro1 protein is shown in SEQ ID NO: 33.
[0431] The nucleotide sequence of the PhuPro1 gene isolated from Paenibacillus hunanensis is set forth as SEQ ID NO: 31. The sequence encoding the predicted native signal peptide is shown in italics:
TABLE-US-00054 TTGAAAAAAACAGTTGGTCTTTTACTTGCAGGTAGCTTGCTCGTTGGTGC TACAACGTCCGCTTTCGCAGCAGAAGCAAATGATCTGGCACCACTCGGTG ATTACACGCCAAAATTGATTACGCAAGCAACAGGCATCACTGGCGCTAGT GGCGATGCTAAAGTATGGAAGTTCCTGGAGAAGCAAAAACGTACCATCGT AACCGATGATGCAGCTTCTGCTGATGTGAAGGAATTGTTTGAGATCACAA AACGTCAATCCGATTCTCAAACCGGTACAGAGCACTATCGCCTGAACCAA ACCTTTAAAGGCATCCCAGTCTATGGCGCAGAGCAAACACTGCACTTTGA CAAATCCGGCAATGTATCTCTGTACATGGGTCAGGTTGTTGAGGATGTGT CCGCTAAACTGGAAGCTTCCGATTCCAAAAAAGGCGTAACTGAGGATGTA TACGCTTCGGATACGAAAAATGATCTGGTAACACCAGAAATCAGCGCTTC TCAAGCCATCTCGATTGCTGAAAAGGATGCAGCTTCCAAAATCGGCTCCC TCGGCGAAGCACAAAAAACGCCAGAAGCGAAGCTGTATATCTACGCTCCT GAGGATCAAGCAGCACGTCTGGCTTATGTGACAGAAGTAAACGTACTGGA GCCATCTCCGCTGCGTACTCGCTATTTTGTAGATGCAAAAACAGGTTCGA TCCTGTTCCAATATGATCTGATTGAGCATGCAACAGGTACAGGTAAAGGG GTACTGGGTGATACCAAGTCCTTCACTGTAGGTACTTCCGGTTCTTCCTA TGTGATGACTGATAGCACGCGTGGAAAAGGTATCCAAACCTACACGGCGT CTAACCGCACATCACTGCCAGGTAGCACTGTAACGAGCAGCAGCAGCACA TTTAACGATCCAGCATCTGTCGATGCCCATGCGTATGCACAAAAAGTATA TGATTTCTACAAATCCAACTTTAACCGCAACAGCATCGACGGTAATGGTC TGGCTATCCGCTCCACTACGCACTATTCCACACGTTATAACAATGCGTTC TGGAATGGTTCCCAAATGGTATACGGTGATGGCGATGGTTCGCAATTCAT CGCATTCTCCGGCGACCTTGACGTAGTAGGTCACGAGCTGACACACGGTG TAACCGAGTACACAGCGAACCTGGAATACTATGGTCAATCCGGTGCACTG AACGAATCCATTTCGGATATCTTTGGTAACACAATCGAAGGTAAAAACTG GATGGTAGGCGATGCGATCTACACACCAGGCGTATCCGGCGATGCTCTTC GCTACATGGATGATCCAACAAAAGGTGGACAACCAGCGCGTATGGCAGAT TACAACAACACAAGCGCTGATAATGGCGGTGTACACACAAACAGTGGTAT CCCGAATAAAGCATACTACTTGCTGGCACAGGGTGGCACATTTGGCGGTG TAAATGTAACAGGTATCGGTCGCTCGCAAGCGATCCAGATCGTTTACCGT GCACTAACATACTACCTGACATCCACATCTAACTTCTCGAACTACCGTTC TGCAATGGTGCAAGCATCTACAGACCTGTACGGTGCAAACTCTACACAAA CAACAGCGGTGAAAAACTCGCTGAGCGCAGTAGGCATTAAC
[0432] The amino acid sequence of the PhuPro1 precursor protein is set forth as SEQ ID NO: 32. The predicted signal sequence is shown in italics, and the predicted pro-peptide is shown in underlined text:
TABLE-US-00055 MKKTVGLLLAGSLLVGATTSAFAAEANDLAPLGDYTPKLITQATGITGAS GDAKVWKFLEKQKRTIVTDDAASADVKELFEITKRQSDSQTGTEHYRLNQ TFKGIPVYGAEQTLHFDKSGNVSLYMGQVVEDVSAKLEASDSKKGVTEDV YASDTKNDLVTPEISASQAISIAEKDAASKIGSLGEAQKTPEAKLYIYAP EDQAARLAYVTEVNVLEPSPLRTRYFVDAKTGSILFQYDLIEHATGTGKG VLGDTKSFTVGTSGSSYVMTDSTRGKGIQTYTASNRTSLPGSTVTSSSST FNDPASVDAHAYAQKVYDFYKSNFNRNSIDGNGLAIRSTTHYSTRYNNAF WNGSQMVYGDGDGSQFIAFSGDLDVVGHELTHGVTEYTANLEYYGQSGAL NESISDIFGNTIEGKNWMVGDAIYTPGVSGDALRYMDDPTKGGQPARMAD YNNTSADNGGVHTNSGIPNKAYYLLAQGGTFGGVNVTGIGRSQAIQIVYR ALTYYLTSTSNFSNYRSAMVQASTDLYGANSTQTTAVKNSLSAVGIN
[0433] The amino acid sequence of the predicted mature form of PhuPro1 is set forth as SEQ ID NO: 33:
TABLE-US-00056 ATGTGKGVLGDTKSFTVGTSGSSYVMTDSTRGKGIQTYTASNRTSLPGST VTSSSSTFNDPASVDAHAYAQKVYDFYKSNFNRNSIDGNGLAIRSTTHYS TRYNNAFWNGSQMVYGDGDGSQFIAFSGDLDVVGHELTHGVTEYTANLEY YGQSGALNESISDIFGNTIEGKNWMVGDAIYTPGVSGDALRYMDDPTKGG QPARMADYNNTSADNGGVHTNSGIPNKAYYLLAQGGTFGGVNVTGIGRSQ AIQIVYRALTYYLTSTSNFSNYRSAMVQASTDLYGANSTQTTAVKNSLSA VGIN
Example 7.2
Expression of Paenibacillus hunanensis Metalloprotease PhuPro1
[0434] The DNA sequence of the propeptide-mature form of PhuPro1 was synthesized and inserted into the Bacillus subtilis expression vector p2JM103BBI (Vogtentanz, Protein Expr Purif, 55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX149(AprE-PhuPro1) (FIG. 7.1). Ligation of this gene encoding the PhuPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3' end of the B. subtilis AprE signal sequence and the 5' end of the predicted PhuPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in FIG. 1, labeled pGX149(AprE-PhuPro1) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in B. subtilis, and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PhuPro1 (SEQ ID NO: 34). The translation product of the synthetic AprE-PhuPro1 gene is shown in SEQ ID NO: 35.
[0435] The pGX149(AprE-PhuPro1) plasmid was then transformed into B. subtilis cells (degU.sup.Hy32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat#232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl2).
[0436] The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl2 and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to a 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above; and the active flow-through fractions were collected and concentrated via 10K Amicon Ultra for further analyses.
[0437] The nucleotide sequence of the synthesized PhuPro1 gene in plasmid pGX149(AprE-PhuPro1) is depicted in SEQ ID NO: 34. The sequence encoding the three residue addition (AGK) is shown in bold:
TABLE-US-00057 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAAT CTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCTGCTGGAAAAGCAG AAGCTAATGATCTTGCCCCGCTTGGCGATTATACACCGAAGCTTATTACA CAGGCAACGGGAATTACAGGCGCATCAGGCGATGCGAAGGTGTGGAAGTT CCTGGAGAAGCAGAAGAGAACGATTGTCACGGACGACGCCGCAAGCGCGG ATGTCAAGGAGCTGTTCGAGATCACGAAGAGACAGAGCGATAGCCAGACG GGAACGGAGCATTACAGACTGAACCAGACGTTCAAGGGCATTCCGGTCTA CGGAGCTGAACAAACGCTGCATTTTGATAAAAGCGGCAACGTCTCACTGT ACATGGGCCAAGTCGTTGAGGACGTTAGCGCCAAACTTGAGGCTAGCGAC AGCAAGAAAGGCGTCACAGAAGATGTCTACGCGTCAGACACGAAAAACGA CCTGGTTACACCGGAAATCTCAGCTTCACAGGCCATCTCAATTGCAGAGA AAGACGCAGCGTCAAAAATCGGCTCACTGGGCGAGGCTCAGAAAACGCCG GAGGCGAAACTTTACATCTACGCCCCTGAGGACCAGGCTGCGAGACTGGC TTACGTGACAGAAGTTAATGTGCTGGAGCCGTCACCGCTTAGAACGAGAT ATTTCGTGGACGCAAAGACGGGCAGCATTCTGTTTCAGTACGATCTTATC GAACACGCGACAGGCACAGGAAAGGGAGTTCTGGGAGACACAAAAAGCTT CACGGTTGGCACGTCAGGCAGCAGCTACGTGATGACAGACAGCACGAGAG GCAAGGGCATTCAAACGTATACAGCGAGCAACAGAACAAGCCTGCCGGGA AGCACAGTCACGAGCTCATCATCAACGTTTAATGACCCGGCCTCAGTGGA TGCTCACGCATACGCGCAGAAAGTGTACGACTTCTACAAAAGCAACTTCA ATAGAAACAGCATCGACGGAAACGGCCTTGCGATCAGAAGCACGACGCAC TACAGCACAAGATACAACAACGCCTTCTGGAACGGCAGCCAAATGGTTTA CGGCGATGGCGACGGATCACAGTTTATCGCATTTAGCGGAGACCTGGACG TCGTTGGCCATGAGCTGACACATGGCGTTACGGAGTACACAGCAAACCTG GAATACTATGGCCAGTCAGGCGCCCTTAACGAGAGCATCAGCGACATTTT TGGCAATACGATCGAAGGAAAGAACTGGATGGTCGGCGACGCAATCTACA CACCGGGCGTTTCAGGCGATGCACTGAGATATATGGACGACCCGACAAAG GGCGGACAGCCGGCCAGAATGGCGGATTACAATAATACGTCAGCAGATAA CGGCGGCGTGCATACAAATAGCGGCATCCCTAACAAAGCATATTACCTGC TTGCGCAAGGAGGAACATTTGGCGGCGTGAATGTTACGGGCATTGGCAGA TCACAAGCGATTCAGATCGTTTACAGAGCGCTGACGTACTACCTTACGAG CACGAGCAATTTTAGCAACTACAGAAGCGCAATGGTGCAGGCAAGCACGG ATCTGTATGGCGCAAATTCAACACAAACGACGGCGGTCAAGAATAGCCTT TCAGCAGTGGGCATTAACTAA
[0438] The amino acid sequence of the PhuPro1 precursor protein expressed from plasmid pGX149(AprE-PhuPro1) is depicted in SEQ ID NO: 35. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text.
TABLE-US-00058 MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKAEANDLAPLGDYTPKLIT QATGITGASGDAKVWKFLEKQKRTIVTDDAASADVKELFEITKRQSDSQT GTEHYRLNQTFKGIPVYGAEQTLHFDKSGNVSLYMGQVVEDVSAKLEASD SKKGVTEDVYASDTKNDLVTPEISASQAISIAEKDAASKIGSLGEAQKTP EAKLYIYAPEDQAARLAYVTEVNVLEPSPLRTRYFVDAKTGSILFQYDLI EHATGTGKGVLGDTKSFTVGTSGSSYVMTDSTRGKGIQTYTASNRTSLPG STVTSSSSTFNDPASVDAHAYAQKVYDFYKSNFNRNSIDGNGLAIRSTTH YSTRYNNAFWNGSQMVYGDGDGSQFIAFSGDLDVVGHELTHGVTEYTANL EYYGQSGALNESISDIFGNTIEGKNWMVGDAIYTPGVSGDALRYMDDPTK GGQPARMADYNNTSADNGGVHTNSGIPNKAYYLLAQGGTFGGVNVTGIGR SQAIQIVYRALTYYLTSTSNFSNYRSAMVQASTDLYGANSTQTTAVKNSL SAVGIN
Example 7.3
Proteolytic Activity of Metalloprotease PhuPro1
[0439] The proteolytic activity of purified metalloprotease PhuPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat#74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H2O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A440) using a SpectraMax 190. Net A440 was calculated by subtracting the A440 of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays. The proteolytic activity is shown as Net A440. The proteolytic assay with azo-casein as the substrate (shown in FIG. 7.2) indicates that PhuPro1 is an active protease.
Example 7.4
pH Profile of Metalloprotease PhuPro1
[0440] With azo-casein as the substrate, the pH profile of metalloprotease PhuPro1 was studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl Milli-Q H2O diluted enzyme (125 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in FIG. 7.3, the optimal pH of PhuPro1 is about 6, with greater than 70% of maximal activity retained between 5 and 8.
Example 7.5
Temperature Profile of Metalloprotease PhuPro1
[0441] The temperature profile of metalloprotease PhuPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 7.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H2O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20˜90° C.) for 5 min. After the incubation, 5 μl of diluted enzyme (50 ppm) or H2O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 7.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in FIG. 7.4 suggests that PhuPro1 showed an optimal temperature at 60° C., and retained greater than 70% of its maximum activity between 45 and 65° C.
Example 7.6
Cleaning Performance of Metalloprotease PhuPro1
[0442] The cleaning performance of PhuPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca2+:Mg2+=3:1) (detergent composition shown in Table 7.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the "Initial performance") using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the "Wash-off"). The sum of two absorbance measurements ("Initial performance" plus "Wash-off") gives the "Total performance", which measures the protease activity on the model stain; and Net A405 was subsequently calculated by subtracting the A405 of the "Total performance" of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 in AT detergent for PhuPro1 is shown in FIGS. 7.5A and 7.5B.
TABLE-US-00059 TABLE 7.1 Composition of AT detergent Concentration Ingredient (mg/ml) MGDA (methylglycinediacetic acid) 0.143 Sodium citrate 1.86 Citric acid* varies Plurafac ® LF 18B (a non-ionic surfactant) 0.029 Bismuthcitrate 0.006 Bayhibit ® S (Phosphonobutantricarboxylic 0.006 acid sodium salt) Acusol ® 587 (a calcium polyphosphate inhibitor) 0.029 PEG 6000 0.043 PEG 1500 0.1 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid.
Example 7.7
Comparison of PhuPro1 to Other Proteases
[0443] A. Identification of Homologous Proteases
[0444] Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PhuPro1 (SEQ ID NO: 33) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 7.2A and 7.2B provide a list of sequences with the percent identity to PhuPro1. The length in Table 7.2 refers to the entire sequence length of the homologous proteases.
TABLE-US-00060 TABLE 7.2A List of sequences with percent identity to PhuPro1 protein identified from the NCBI non-redundant protein database PID to Accession # PhuPro1 Organism Length P00800 55 Bacillus thermoproteolyticus 548 AAB02774.1 55 Geobacillus stearothermophilus 552 EJS73098.1 56 Bacillus cereus BAG2X1-3 566 BAD60997.1 56 Bacillus megaterium 562 ZP_04216147.1 57 Bacillus cereus Rock3-44 566 YP_893436.1 56 Bacillus thuringiensis str. 566 Al Hakam ZP_08640523.1 58 Brevibacillus laterosporus 564 ZP_09069194.1 59 Paenibacillus larvae subsp. 502 larvae B-3650 YP_002770810.1 60 Brevibacillus brevis 528 ZP_08511445.1 61 Paenibacillus sp. HGF7 525 P43263 61 Brevibacillus brevis 527 ZP_09775365.1 62 Paenibacillus sp. Aloe-11 580 ZP_09077634.1 66 Paenibacillus elgii B69 524 P29148 68 NPRE_PAEPO 590 ZP_09775364.1 69 Paenibacillus sp. Aloe-11 593 ZP_10241030.1 69 Paenibacillus peoriae 593 KCTC 3763 YP_005073223.1 69 Paenibacillus terrae HPL-003 591
TABLE-US-00061 TABLE 7.2B List of sequences with percent identity to PhuPro1 protein identified from the Genome Quest Patent database PID to Patent ID # PhuPro1 Organism Length WO2012110562-0003 56.23 Geobacillus 319 stearothermophilus US6518054-0001 56.55 Bacillus sp. 319 JP2002272453-0002 56.69 Bacillus megaterium 562 US20090123467-0184 56.73 Bacillus anthracis 566 US6103512-0003 56.87 319 EP0867512-0002 56.96 316 WO2012110562-0005 57.1 Bacillus cereus 320 WO2012110563-0005 58.06 Bacillus cereus 320 US20120107907-0187 68.44 Bacillus polymyxa 302
[0445] B. Alignment of Homologous Protease Sequences
[0446] The amino acid sequence of predicted mature PhuPro1 (SEQ ID NO: 33) protein was aligned with Proteinase T (P00800, Bacillus thermoproteolyticus), and protease from Paenibacillus terrae HPL-003 (YP_005073223.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters. FIG. 7.6 shows the alignment of PhuPro1 with these protease sequences.
[0447] C. Phylogenetic Tree
[0448] A phylogenetic tree for full length sequence of PhuPro1 (SEQ ID NO: 2) was built using sequences of representative homologs from Table 2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. Mol Biol. Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in FIG. 7.7.
Example 7.8
Terg-o-Tometer Performance Evaluation of PhuPro1
[0449] The wash performance of PhuPro1 was tested in a laundry detergent application using a Terg-o-Tometer (Instrument Marketing Services, Inc, Fairfield, N.J.). The performance evaluation was conducted at 32° C. and 16° C. The soil load consisted of two of each of the following stain swatches: EMPA116 Blood, Milk, Ink on cotton (Test materials AG, St. Gallen, Switzerland), EMPA117 Blood, Milk, Ink on polycotton (Test materials AG, St. Gallen, Switzerland), EMPA112 Cocoa on cotton (Test materials AG, St. Gallen, Switzerland), and CFT C-10 Pigment, Oil, and Milk content on cotton (Center for Testmaterials BV, Vlaardingen, Netherlands), plus extra white interlock knit fabric to bring the total fabric load to 40 g per beaker of the Terg-o-Tometer, which was filled with 1 L of deionized water. The water hardness was adjusted to 6 grains per gallon, and the pH in the beaker was buffered with 5 mM HEPES, pH 8.2. Heat inactivated Tide Regular HDL (Proctor & Gamble), a commercial liquid detergent purchased in a local US supermarket, was used at 0.8 g/L. The detergent was inactivated before use by treatment at 92° C. in a water bath for 2-3 hours followed by cooling to room temperature. Heat inactivation of commercial detergents serves to destroy the activity of enzymatic components while retaining the properties of the non-enzymatic components. Enzyme activity in the heat inactivated detergent was measured using the Suc-AAPF-pNA assay for measuring protease activity. The Purafect® Prime HA, (Genencor Int'l) and PhuPro1 proteases were each added to final concentrations of 1 ppm. A control sample with no enzyme was included. The wash time was 12 minutes. After the wash treatment, all swatches were rinsed for 3 minutes and machine-dried at low heat.
[0450] Four of each type of swatch were measured before and after treatment by optical reflectance using a Tristimulus Minolta Meter CR-400. The difference in the L, a, b values was converted to total color difference (dE), as defined by the CIE-LAB color space. Cleaning of the stains is expressed as percent stain removal index (% SRI) by taking a ratio between the color difference before and after washing, and comparing it to the difference of unwashed soils (before wash) to unsoiled fabric, and averaging the eight values obtained by reading two different regions of each washed swatch. Cleaning performances of PhuPro1 and Purafect® Prime HA proteases at 32° C. are shown in Tables 7.8A and FIG. 7.8A and at 16° C. are shown in Table 7.8B and FIG. 7.8B.
TABLE-US-00062 TABLE 7.8A Cleaning performance of PhuPro1 at 32° C. Purafect Prime Purafect Prime HA PhuPro1 HA PhuPro1 Average 95CI Average 95CI Average 95CI Average 95CI ppm % SRI [% SRI % SRI [% SRI % SRI [% SRI % SRI [% SRI enzyme (dE) (dE)] (dE) (dE)] (dE) (dE)] (dE) (dE)] EMPA-116 EMPA-117 0 0.25 0.02 0.25 0.02 0.19 0.02 0.19 0.02 0.2 0.31 0.02 0.31 0.01 0.31 0.03 0.32 0.04 0.5 0.34 0.02 0.33 0.03 0.34 0.02 0.37 0.02 1 0.35 0.03 0.36 0.02 0.38 0.03 0.42 0.03 1.5 0.36 0.02 0.37 0.03 0.35 0.03 0.43 0.03 EMPA-112 CFT C-10 0 0.15 0.03 0.15 0.03 0.07 0.01 0.07 0.01 0.2 0.17 0.04 0.14 0.02 0.11 0.01 0.15 0.01 0.5 0.19 0.02 0.19 0.04 0.13 0.01 0.16 0.03 1 0.20 0.03 0.22 0.03 0.17 0.01 0.17 0.01 1.5 0.24 0.03 0.25 0.04 0.17 0.02 0.20 0.02
TABLE-US-00063 TABLE 7.8B Cleaning performance of PhuPro1 at 16° C. Purafect Prime Purafect Prime HA PhuPro1 HA PhuPro1 Average 95CI Average 95CI Average 95CI Average 95CI ppm % SRI [% SRI % SRI [% SRI % SRI [% SRI % SRI [% SRI enzyme (dE) (dE)] (dE) (dE)] (dE) (dE)] (dE) (dE)] EMPA-116 EMPA-117 0 0.14 0.02 0.14 0.02 0.12 0.01 0.12 0.01 0.2 0.19 0.02 0.17 0.03 0.17 0.02 0.14 0.03 0.5 0.22 0.03 0.28 0.04 0.20 0.03 0.22 0.01 1 0.24 0.02 0.26 0.02 0.20 0.01 0.24 0.04 1.5 0.23 0.03 0.26 0.03 0.23 0.02 0.25 0.02 EMPA-112 CFT C-10 0 0.09 0.03 0.09 0.03 0.07 0.01 0.07 0.01 0.2 0.07 0.01 0.09 0.02 0.08 0.02 0.06 0.01 0.5 0.11 0.02 0.12 0.03 0.10 0.01 0.09 0.01 1 0.11 0.02 0.12 0.02 0.13 0.01 0.15 0.01 1.5 0.13 0.03 0.19 0.03 0.13 0.01 0.11 0.01
Example 8.1
Cloning of Paenibacillus amylolyticus Metalloprotease PamPro1
[0451] A strain (DSM11747) of Paenibacillus amylolyticus was selected as a potential source of enzymes which may be useful in various industrial applications. Genomic DNA for sequencing was obtained by first growing the strain on Heart Infusion agar plates (Difco) at 37° C. for 24 hr. Cell material was scraped from the plates and used to prepare genomic DNA with the ZF Fungal/Bacterial DNA miniprep kit from Zymo (Cat No. D6005). The genomic DNA was used for genome sequencing. The entire genome of the Paenibacillus amylolyticus strain was sequenced by BaseClear (Leiden, The Netherlands) using the Illumina's next generation sequencing technology. After assembly of the data, contigs were annotated by BioXpr (Namur, Belgium). One of the genes identified after annotation in Paenibacillus amylolyticus encodes a metalloprotease and the sequence of this gene, called PamPro1, is provided in SEQ ID NO: 36. The corresponding protein encoded by the PamPro1 gene is shown in SEQ ID NO: 37. At the N-terminus, the protein has a signal peptide with a length of 25 amino acids as predicted by SignalP version 4.0 (Nordahl Petersen et al. (2011) Nature Methods, 8:785-786). The presence of a signal sequence suggests that PamPro1 is a secreted enzyme. The propeptide region was predicted based on protein sequence alignment with the Paenibacillus polymyxa Npr protein (Takekawa et al. (1991) Journal of Bacteriology, 173 (21): 6820-6825). The predicted mature region of PamPro1 protein is shown in SEQ ID NO: 3.
[0452] The nucleotide sequence of the PamPro1 gene isolated from Paenibacillus amylolyticus is set forth as SEQ ID NO: 36. The sequence encoding the predicted native signal peptide is shown in italics:
TABLE-US-00064 ATGAAATTCGCCAAAGTTATGCCAACAATTCTTGGAGGAGCTCTTTTGCT CGCTTCCGTATCCTCTGCTACTGCAGCTCCAGTGTCTGATCAATCCATTC CACTTCAGGCCCCTTATGCCTCTGAGGGGGGTATTCCATTGAACAGTGGA ACAGATGACACTATCTTTAATTATCTTGGACAGCAGGAACAATTTCTGAA TTCCGATGTGAAATCCCAGCTCAAAATTGTCAAAAGAAACACAGATACAT CTGGCGTAAGACACTTCCGCCTGAAACAGTATATTAAAGGTATCCCGGTT TATGGTGCAGAACAGACGGTCCACCTGGACAAAACCGGAGCCGTGAGCTC CGCACTTGGCGATCTTCCACCGATTGAAGAGCAGGCCATTCCGAATGATG GTGTAGCCGAGATCAGCGGAGAAGACGCGATCCAGATTGCAACCGAAGAA GCAACCTCCCGGATTGGAGAGCTTGGTGCCGCGGAAATCACGCCTCAAGC TGAATTGAACATCTATCATCATGAAGAAGATGGTCAGACATATCTGGTTT ACATTACGGAAGTAAACGTACTGGAACCTGCCCCTCTACGGACCAAATAT TTCATTAACGCAGTGGATGGCAGTATCGTATCCCAGTTTGACCTCATTAA CTTCGCTACTGGAACAGGTACAGGTGTACTCGGTGATACCAAAACCCTGA CAACCACCCAATCCGGCAGCACCTTCCAACTGAAAGACACCACTCGTGGC AATGGCATCCAAACGTATACGGCAAACAATGGCTCCTCACTGCCTGGTAG CTTGCTTACAGATTCGGATAATGTATGGACCGATCGTGCAGGTGTAGATG CTCATGCTCATGCCGCTGCTACGTATGATTTCTACAAAAACAAATTCAAC CGTAACGGTATTAATGGTAACGGATTGTTGATCAGATCAACCGTGCACTA CGGCTCCAATTACAATAACGCCTTCTGGAACGGGGCACAGATTGTCTTTG GTGACGGAGATGGAACGATGTTCCGATCCCTGTCTGGTGATCTGGATGTT GTGGGTCATGAATTGACGCATGGTGTTATTGAATATACAGCCAATCTGGA ATATCGCAATGAACCAGGTGCACTCAATGAAGCCTTTGCCGATATTTTCG GTAATACGATCCAAAGCAAAAACTGGCTGCTCGGTGATGATATCTACACA CCTAACACTCCAGGAGATGCGCTGCGCTCCCTCTCCAACCCTACATTGTA TGGTCAACCTGACAAATACAGCGATCGCTACACAGGCTCACAGGACAACG GCGGTGTCCATATCAACAGTGGTATCATCAATAAAGCCTATTTCCTTGCT GCTCAAGGCGGAACACATAATGGTGTGACTGTTACCGGAATCGGCCGGGA TAAAGCGATCCAGATTTTCTACAGCACACTGGTGAACTACCTGACACCAA CGTCCAAATTTGCCGCTGCCAAAACAGCTACCATTCAAGCAGCCAAAGAT CTGTACGGAGCAACTTCCGCTGAAGCTACTGCTATTACCAAAGCATATCA AGCTGTAGGCCTG
[0453] The amino acid sequence of the PamPro1 precursor protein is set forth as SEQ ID NO: 37. The predicted signal sequence is shown in italics, and the predicted propeptide is shown in underlined text:
TABLE-US-00065 MKFAKVMPTILGGALLLASVSSATAAPVSDQSIPLQAPYASEGGIPLNSG TDDTIFNYLGQQEQFLNSDVKSQLKIVKRNTDTSGVRHFRLKQYIKGIPV YGAEQTVHLDKTGAVSSALGDLPPIEEQAIPNDGVAEISGEDAIQIATEE ATSRIGELGAAEITPQAELNIYHHEEDGQTYLVYITEVNVLEPAPLRTKY FINAVDGSIVSQFDLINFATGTGTGVLGDTKTLTTTQSGSTFQLKDTTRG NGIQTYTANNGSSLPGSLLTDSDNVWTDRAGVDAHAHAAATYDFYKNKFN RNGINGNGLLIRSTVHYGSNYNNAFWNGAQIVFGDGDGTMFRSLSGDLDV VGHELTHGVIEYTANLEYRNEPGALNEAFADIFGNTIQSKNWLLGDDIYT PNTPGDALRSLSNPTLYGQPDKYSDRYTGSQDNGGVHINSGIINKAYFLA AQGGTHNGVTVTGIGRDKAIQIFYSTLVNYLTPTSKFAAAKTATIQAAKD LYGATSAEATAITKAYQAVGL
[0454] The amino acid sequence of the predicted mature form of PamPro1 is set forth as SEQ ID NO: 38:
TABLE-US-00066 ATGTGTGVLGDTKTLTTTQSGSTFQLKDTTRGNGIQTYTANNGSSLPGSL LTDSDNVWTDRAGVDAHAHAAATYDFYKNKFNRNGINGNGLLIRSTVHYG SNYNNAFWNGAQIVFGDGDGTMFRSLSGDLDVVGHELTHGVIEYTANLEY RNEPGALNEAFADIFGNTIQSKNWLLGDDIYTPNTPGDALRSLSNPTLYG QPDKYSDRYTGSQDNGGVHINSGIINKAYFLAAQGGTHNGVTVTGIGRDK AIQIFYSTLVNYLTPTSKFAAAKTATIQAAKDLYGATSAEATAITKAYQA VGL
Example 8.2
Expression of Paenibacillus amylolyticus Metalloprotease PamPro1
[0455] The DNA sequence of the propeptide-mature form of PamPro1 was synthesized and inserted into the Bacillus subtilis expression vector p2JM103BBI (Vogtentanz, Protein Expr Purif, 55:40-52, 2007) by Generay (Shanghai, China), resulting in plasmid pGX146(AprE-PamPro1) (FIG. 1). Ligation of this gene encoding the PamPro1 protein into the digested vector resulted in the addition of three codons (Ala-Gly-Lys) between the 3' end of the B. subtilis AprE signal sequence and the 5' end of the predicted PamPro1 native propeptide. The gene has an alternative start codon (GTG). The resulting plasmid shown in FIG. 8.1, labeled pGX146(AprE-PamPro1) contains an AprE promoter, an AprE signal sequence used to direct target protein secretion in B. subtilis, and the synthetic nucleotide sequence encoding the predicted propeptide and mature regions of PamPro1 (SEQ ID NO: 39). The translation product of the synthetic AprE-PamPro1 gene is shown in SEQ ID NO: 40.
[0456] The pGX146(AprE-PamPro1) plasmid was then transformed into B. subtilis cells (degU.sup.Hy32, ΔscoC) and the transformed cells were spread on Luria Agar plates supplemented with 5 ppm Chloramphenicol and 1.2% skim milk (Cat#232100, Difco). Colonies with the largest clear halos on the plates were selected and subjected to fermentation in a 250 ml shake flask with MBD medium (a MOPS based defined medium, supplemented with additional 5 mM CaCl2).
[0457] The broth from the shake flasks was concentrated and buffer-exchanged into the loading buffer containing 20 mM Tris-HCl (pH 8.5), 1 mM CaCl2 and 10% propylene glycol using a VivaFlow 200 ultra filtration device (Sartorius Stedim). After filtering, this sample was applied to an 80 ml Q Sepharose High Performance column pre-equilibrated with the loading buffer above; and the active flow-through fractions were collected and concentrated. The sample was loaded onto a 320 ml Superdex 75 gel filtration column pre-equilibrated with the loading buffer described above containing 0.15 M NaCl. The corresponding active purified protein fractions were further pooled and concentrated via 10K Amicon Ultra for further analyses.
[0458] The nucleotide sequence of the synthesized PamPro1 gene in plasmid pGX146(AprE-PamPro1) is depicted in SEQ ID NO: 39. The sequence encoding the three residue addition (AGK) is shown in bold:
TABLE-US-00067 GTGAGAAGCAAAAAATTGTGGATCAGCTTGTTGTTTGCGTTAACGTTAAT CTTTACGATGGCGTTCAGCAACATGAGCGCGCAGGCTGCTGGAAAAGCTC CGGTTAGCGACCAGTCAATCCCTCTTCAAGCACCGTATGCCAGCGAAGGA GGCATTCCGCTTAACAGCGGCACGGACGACACGATTTTCAATTACCTGGG CCAACAGGAGCAGTTCCTGAACAGCGACGTCAAGAGCCAGCTGAAGATCG TCAAAAGAAACACAGACACATCAGGCGTGAGACACTTCAGACTGAAGCAA TACATCAAGGGCATCCCGGTTTATGGCGCTGAACAAACGGTTCACCTGGA CAAAACAGGCGCAGTTTCATCAGCACTGGGAGATCTGCCGCCGATTGAAG AGCAAGCAATCCCGAATGATGGAGTTGCGGAAATTAGCGGCGAGGATGCA ATCCAAATCGCGACGGAGGAGGCTACATCAAGAATTGGAGAACTTGGCGC AGCGGAGATTACACCGCAGGCTGAACTGAACATCTATCACCATGAGGAAG ACGGCCAGACGTACCTGGTTTACATTACGGAAGTGAACGTGCTGGAACCG GCACCTCTGAGAACAAAGTACTTTATCAACGCGGTTGACGGCAGCATCGT CTCACAGTTCGACCTGATTAACTTCGCCACGGGAACAGGAACGGGCGTTC TTGGAGACACAAAGACGCTGACGACGACGCAGTCAGGCAGCACATTCCAG CTGAAGGACACAACAAGAGGCAACGGCATCCAAACGTACACGGCGAACAA TGGATCATCACTGCCGGGCTCACTGCTGACGGATTCAGATAACGTGTGGA CGGATAGAGCTGGCGTTGACGCGCATGCTCACGCTGCTGCGACGTACGAC TTCTACAAGAACAAGTTCAACAGAAACGGCATTAACGGAAATGGCCTGCT GATCAGAAGCACGGTGCATTATGGCTCAAACTACAACAACGCTTTTTGGA ACGGCGCACAGATCGTGTTTGGCGACGGCGATGGCACAATGTTTAGAAGC CTGTCAGGAGACCTGGATGTGGTGGGCCACGAACTGACGCACGGCGTGAT CGAGTATACGGCGAACCTTGAATATAGAAACGAGCCGGGAGCACTGAATG AGGCGTTCGCGGACATTTTCGGCAACACAATCCAGAGCAAAAACTGGCTG CTGGGCGACGATATCTATACACCGAACACACCGGGCGATGCACTGAGATC ACTGTCAAATCCGACGCTGTATGGCCAACCGGATAAGTACTCAGACAGAT ATACGGGCAGCCAAGACAATGGCGGCGTTCACATCAACTCAGGCATCATC AACAAGGCTTACTTCCTTGCGGCCCAAGGAGGAACACATAACGGCGTTAC AGTTACAGGCATTGGCAGAGACAAGGCGATCCAGATCTTTTACAGCACGC TGGTGAACTACCTGACACCTACGTCAAAGTTTGCCGCAGCGAAAACAGCA ACAATTCAGGCGGCTAAAGACCTGTACGGAGCGACATCAGCCGAGGCCAC AGCAATTACAAAAGCATATCAAGCAGTTGGCCTTTAA
[0459] The amino acid sequence of the PamPro1 precursor protein expressed from plasmid pGX146(AprE-PamPro1) is depicted in SEQ ID NO: 40. The predicted signal sequence is shown in italics, the three residue addition (AGK) is shown in bold, and the predicted pro-peptide is shown in underlined text.
TABLE-US-00068 MRSKKLWISLLFALTLIFTMAFSNMSAQAAGKAPVSDQSIPLQAPYASEG GIPLNSGTDDTIFNYLGQQEQFLNSDVKSQLKIVKRNTDTSGVRHFRLKQ YIKGIPVYGAEQTVHLDKTGAVSSALGDLPPIEEQAIPNDGVAEISGEDA IQIATEEATSRIGELGAAEITPQAELNIYHHEEDGQTYLVYITEVNVLEP APLRTKYFINAVDGSIVSQFDLINFATGTGTGVLGDTKTLTTTQSGSTFQ LKDTTRGNGIQTYTANNGSSLPGSLLTDSDNVWTDRAGVDAHAHAAATYD FYKNKFNRNGINGNGLLIRSTVHYGSNYNNAFWNGAQIVFGDGDGTMFRS LSGDLDVVGHELTHGVIEYTANLEYRNEPGALNEAFADIFGNTIQSKNWL LGDDIYTPNTPGDALRSLSNPTLYGQPDKYSDRYTGSQDNGGVHINSGII NKAYFLAAQGGTHNGVTVTGIGRDKAIQIFYSTLVNYLTPTSKFAAAKTA TIQAAKDLYGATSAEATAITKAYQAVGL
Example 8.3
Proteolytic Activity of Metalloprotease PamPro1
[0460] The proteolytic activity of purified metalloprotease PamPro1 was measured in 50 mM Tris (pH 7), using azo-casein (Cat#74H7165, Megazyme) as a substrate. Prior to the reaction, the enzyme was diluted with Milli-Q water (Millipore) to specific concentrations. The azo-casein was dissolved in 100 mM Tris buffer (pH 7) to a final concentration of 1.5% (w/v). To initiate the reaction, 50 μl of the diluted enzyme (or Milli-Q H2O alone as the blank control) was added to the non-binding 96-well Microtiter Plate (96-MTP) (Corning Life Sciences, #3641) placed on ice, followed by the addition of 50 μl of 1.5% azo-casein. After sealing the 96-MTP, the reaction was carried out in a Thermomixer (Eppendorf) at 40° C. and 650 rpm for 10 min. The reaction was terminated by adding 100 μl of 5% Trichloroacetic Acid (TCA). Following equilibration (5 min at the room temperature) and subsequent centrifugation (2000 g for 10 min at 4° C.), 120 μl supernatant was transferred to a new 96-MTP, and absorbance of the supernatant was measured at 440 nm (A440) using a SpectraMax 190. Net A440 was calculated by subtracting the A440 of the blank control from that of enzyme, and then plotted against different protein concentrations (from 1.25 ppm to 40 ppm). Each value was the mean of triplicate assays. The proteolytic activity is shown as Net A440. The proteolytic assay with azo-casein as the substrate (shown in FIG. 8.2) indicates that PamPro1 is an active protease.
Example 8.4
pH Profiles of Metalloprotease PamPro1
[0461] With azo-casein as the substrate, the pH profiles of metalloprotease PamPro1 were studied in 12.5 mM acetate/Bis-Tris/HEPES/CHES buffer with different pH values (ranging from pH 4 to 11). To initiate the assay, 50 μl of 25 mM acetate/Bis-Tris/HEPES/CHES buffer with a specific pH was first mixed with 2 μl Milli-Q H2O diluted enzyme (125 ppm) in a 96-MTP placed on ice, followed by the addition of 48 μl of 1.5% (w/v) azo-casein prepared in H2O. The reaction was performed and analyzed as described in Example 8.3. Enzyme activity at each pH was reported as the relative activity, where the activity at the optimal pH was set to be 100%. The pH values tested were 4, 5, 6, 7, 8, 9, 10 and 11. Each value was the mean of triplicate assays. As shown in FIG. 8.3, the optimal pH of PamPro1 is about 8, with greater than 70% of maximal activity retained between 7 and 9.5.
Example 8.5
Temperature Profile of Metalloprotease PamPro1
[0462] The temperature profile of metalloprotease PamPro1 was analyzed in 50 mM Tris buffer (pH 7) using the azo-casein assays. The enzyme sample and azo-casein substrate were prepared as in Example 8.3. Prior to the reaction, 50 μl of 1.5% azo-casein and 45 μl Milli-Q H2O were mixed in a 200 μl PCR tube, which was then subsequently incubated in a Peltier Thermal Cycler (BioRad) at desired temperatures (i.e. 20˜90° C.) for 5 min. After the incubation, 5 μl of diluted enzyme (50 ppm) or H2O (the blank control) was added to the substrate mixture, and the reaction was carried out in the Peltier Thermal Cycle for 10 min at different temperatures. To terminate the reaction, each assay mixture was transferred to a 96-MTP containing 100 μl of 5% TCA per well. Subsequent centrifugation and absorbance measurement were performed as described in Example 8.3. The activity was reported as the relative activity, where the activity at the optimal temperature was set to be 100%. The tested temperatures are 20, 30, 40, 50, 60, 70, 80, and 90° C. Each value was the mean of duplicate assays (the value varies no more than 5%). The data in FIG. 8.4 suggest that PamPro1 showed an optimal temperature at about 50° C., and retained greater than 70% of its maximum activity between 45 and 55° C.
Example 8.6
Cleaning Performance of Metalloprotease PamPro1
[0463] The cleaning performance of PamPro1 was tested using PA-S-38 (egg yolk, with pigment, aged by heating) microswatches (CFT-Vlaardingen, The Netherlands) at pH 6 and 8 using a model automatic dishwashing (ADW) detergent. Prior to the reaction, purified protease samples were diluted with a dilution solution containing 10 mM NaCl, 0.1 mM CaCl2, 0.005% TWEEN® 80 and 10% propylene glycol to the desired concentrations. The reactions were performed in AT detergent with 100 ppm water hardness (Ca2+:Mg2+=3:1) (detergent composition shown in Table 8.1). To initiate the reaction, 180 μl of the AT detergent buffered at pH 6 or pH 8 was added to a 96-MTP placed with PA-S-38 microswatches, followed by the addition of 20 μl of diluted enzymes (or the dilution solution as the blank control). The 96-MTP was sealed and incubated in an incubator/shaker for 30 min at 50° C. and 1150 rpm. After incubation, 100 μl of wash liquid from each well was transferred to a new 96-MTP, and its absorbance was measured at 405 nm (referred here as the "Initial performance") using a spectrophotometer. The remaining wash liquid in the 96-MTP was discarded and the microswatches were rinsed once with 200 μl water. Following the addition of 180 μl of 0.1 M CAPS buffer (pH 10), the second incubation was carried out in the incubator/shaker at 50° C. and 1150 rpm for 10 min. One hundred microliters of the resulting wash liquid was transferred to a new 96-MTP, and its absorbance measured at 405 nm (referred here as the "Wash-off"). The sum of two absorbance measurements ("Initial performance" plus "Wash-off") gives the "Total performance", which measures the protease activity on the model stain; and Net A405 was subsequently calculated by subtracting the A405 of the "Total performance" of the blank control from that of the enzyme. Dose response in cleaning the PA-S-38 microswatches at pH 6 and pH 8 in AT dish detergent for PamPro1 is shown in FIGS. 5A and 5B.
TABLE-US-00069 TABLE 8.1 Composition of AT dish detergent formula with bleach Concentration Ingredient (mg/ml) MGDA (methylglycinediacetic acid) 0.143 Sodium citrate 1.86 Citric acid* varies PAP (peracid N,N-phthaloylaminoperoxycaproic acid) 0.057 Plurafac ® LF 18B (a non-ionic surfactant) 0.029 Bismuthcitrate 0.006 Bayhibit ® S (Phosphonobutantricarboxylic 0.006 acid sodium salt) Acusol ® 587 (a calcium polyphosphate inhibitor) 0.029 PEG 6000 0.043 PEG 1500 0.1 *The pH of the AT formula detergent is adjusted to the desired value (pH 6 or 8) by the addition of 0.9M citric acid.
Example 8.7
Comparison of PamPro1 to Other Proteases
A. Identification of Homologous Proteases
[0464] Homologs were identified by a BLAST search (Altschul et al., Nucleic Acids Res, 25:3389-402, 1997) against the NCBI non-redundant protein database and the Genome Quest Patent database with search parameters set to default values. The predicted mature protein amino acid sequence for PamPro1 (SEQ ID NO: 38) was used as the query sequence. Percent identity (PID) for both search sets is defined as the number of identical residues divided by the number of aligned residues in the pairwise alignment. Tables 8.2A and 8.2B provide a list of sequences with the percent identity to PamPro1. The length in Table 8.2 refers to the entire sequence length of the homologous proteases.
TABLE-US-00070 TABLE 8.2A List of sequences with percent identity to PamPro1 protein identified from the NCBI non-redundant protein database PID to Accession # PamPro1 Organism Length P23384 56 Bacillus caldolyticus 544 P00800 56 Bacillus thermoproteolyticus 548 ZP_08640523.1 57 Brevibacillus laterosporus 564 LMG 15441 BAA06144.1 57 Lactobacillus sp. 566 YP_003872180.1 58 Paenibacillus polymyxa E681 587 ZP_04149724.1 59 Bacillus pseudomycoides DSM 566 12442 EJR46541.1 60 Bacillus cereus VD107 566 YP_001373863.1 60 Bacillus cytotoxicus NVH 565 391-98 ZP_10738945.1 61 Brevibacillus sp. CF112 528 YP_004646155.1 61 Paenibacillus mucilaginosus 525 KNP414 ZP_02326602.1 62 Paenibacillus larvae subsp. 520 larvae BRL-230010 P43263 63 Brevibacillus brevis 527 ZP_09775365.1 64 Paenibacillus sp. Aloe-11 580 ZP_09077634.1 65 Paenibacillus elgii B69 529 ZP_09071078.1 68 Paenibacillus larvae 529 subsp. larvae B-3650 ZP_08511445.1 69 Paenibacillus sp. HGF7 525 YP_005073223.1 70 Paenibacillus terrae HPL-003 591 YP_003948511.1 71 Paenibacillus polymyxa SC2 592 ZP_10241030.1 71 Paenibacillus peoriae KCTC 593 3763
TABLE-US-00071 TABLE 8.2B List of sequences with percent identity to PamPro1 protein identified from the Genome Quest Patent database PID to Patent # PamPro1 Organism Length US7335504-0030 56.63 Bacillus 316 thermoproteolyticus US20120107907-0184 56.91 Bacillus caldoyticus 319 JP2006124323-0003 56.96 Bacillus 316 thermoproteolyticus JP1993199872-0001 56.96 Bacillus sp. 316 JP1997000255-0001 56.96 empty 548 US6518054-0001 57.23 Bacillus sp. 319 US20120107907-0176 57.23 Bacillus stearothermophilis 548 US8114656-0183 57.28 Bacillus stearothermophilis 316 US20120009651-0002 57.28 Geobacillus 548 caldoproteolyticus JP2011103791-0020 57.28 Geobacillus 552 stearothermophilus WO2012110562-0006 57.88 Bacillus megaterium 320 EP2390321-0178 57.88 Bacillus thuringiensis 566 US6518054-0002 57.93 Bacillus sp. 316 WO2012110562-0007 58.25 Bacillus cereus 320 JP1995184649-0001 58.52 Lactobacillus sp. 566 EP2178896-0184 58.52 Bacillus anthracis 566 EP2390321-0195 59.55 Bacillus cereus 317 WO2012110563-0005 59.87 Bacillus cereus 320 US20080293610-0186 63.25 Bacillus brevis 304 JP2005229807-0018 71.19 Paenibacillus polymyxa 566 US8114656-0187 71.43 Bacillus polymyxa 302
B. Alignment of Homologous Protease Sequences
[0465] The amino acid sequence of the predicted mature PamPro1 (SEQ ID NO: 38) was aligned with thermolysin (P00800, Bacillus thermoproteolyticus), and protease from Paenibacillus peoriae KCTC 3763 (YP_005073223.1) using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters. FIG. 8.6 shows the alignment of PamPro1 with these protease sequences.
C. Phylogenetic Tree
[0466] A phylogenetic tree for full length sequences of PamPro1 (SEQ ID NO: 37) was built using sequences of representative homologs from Table 8.2A and the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. Mol Biol. Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in FIG. 8.7.
Example 9
Comparison of the Various Paenibacillus Metalloproteases with Other Bacterial Metalloprotease Homologs
A. Alignment of Homologous Protease Sequences
[0467] The amino acid sequence of the predicted mature sequences for the Paenibacillus proteases described in Examples 1.1 to 8.7 were aligned with related bacterial metalloproteases using CLUSTALW software (Thompson et al., Nucleic Acids Research, 22:4673-4680, 1994) with the default parameters. FIG. 9.1 shows the alignment of the various Paenibacillus metalloproteases with other bacterial metalloprotease homologs.
B. Phylogenetic Tree
[0468] A phylogenetic tree for full length sequences of the metalloproteases aligned in FIG. 9.1 was created using the Neighbor Joining method (NJ) (Saitou, N.; and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing Guide Trees. Mol Biol. Evol. 4, 406-425). The NJ method works on a matrix of distances between all pairs of sequences to be analyzed. These distances are related to the degree of divergence between the sequences. The phylodendron-phylogenetic tree printer software (http://iubio.bio.indiana.edu/treeapp/treeprint-form.html) was used to display the phylogenetic tree shown in FIG. 9.2, where one can observe the clustering of the sequences from Paenibacillus genus.
[0469] While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein can be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.
Sequence CWU
1
1
6811785DNAPaenibacillus sp.misc_feature(1)..(1785)nucleotide sequence of
the PspPro3 gene isolated from Paenibacillus sp. 1atgttaatga
aaaaagtatg ggtttcgctt cttggaggag cgatgttatt agggtctgta 60gcgtctggtg
catcagcagc ggagagttcc gtttcggggc cggctcagct tacgccaacc 120ttccatgccg
aacaatggaa agcaccttca tcggtatcgg gtgatgacat cgtatggagc 180tatttaaatc
ggcaaaagaa aacgttgctg ggtacggaca gcaccagtgt ccgtgatcaa 240ttccgtatcg
tagatcgcac aagcgacaaa tccggcgtga gccattatcg gctgaagcaa 300tatgtaaacg
gaattcccgt atatggagct gaacagacca ttcatgtggg caaatccggt 360gaagtgacct
cttatctggg agccgtgatt actgaggatc agcaagaaga agctacgcaa 420ggtacaactc
cgaaaatcag cgcttctgaa gcggtccata ccgcatatca ggaggcagct 480acacgggttc
aagccctccc tacctccgat gatacgattt ctaaagatgc ggaggagcca 540agcagtgtaa
gcaaagacac ttactccgaa gcagctaaca acggaaaaac gagttctgtt 600gaaaaggaca
agctcagcct tgagaaagcg gctgacctga aagatagcaa aattgaagcg 660gtggaggcag
agccaaactc cattgccaaa atcgccaacc tgcagcctga ggtagatcct 720aaagccgaac
tatatttcta tgcgaagggc gatgcattgc agctggttta tgtgactgag 780gttaatattt
tgcagcctgc gccgctgcgt acacgctaca tcattgacgc caatgatggc 840aaaatcgtat
cccagtatga catcattaat gaagcgacag gcacaggcaa aggtgtactc 900ggtgatacca
aaacattcaa cactactgct tccggcagca gctaccagtt aagagatacg 960actcgcggga
atggaatcgt gacttacacg gcctccaacc gtcaaagcat cccaggtacg 1020atcctgaccg
atgccgataa cgtatggaat gatccagccg gcgtggatgc ccacgcttat 1080gcagccaaaa
cctatgatta ttataaggaa aagttcaatc gcaacagcat tgacggacga 1140ggcctgcagc
tccgttcgac agttcattac ggcaatcgtt acaacaacgc cttctggaac 1200ggctcccaaa
tgacttatgg agacggagac ggcaccacat ttatcgcttt tagcggtgat 1260ccggatgtag
ttggtcatga actcacacac ggtgttacgg agtatacttc caatttggaa 1320tattacggag
aatccggtgc gttgaacgag gccttctcgg acatcatcgg caatgacatc 1380cagcgtaaaa
actggcttgt aggcgatgat atttacacgc cacgcattgc gggtgatgca 1440cttcgttcta
tgtccaatcc tacgctgtac gatcaaccgg atcactattc gaacttgtac 1500agaggcagct
ccgataacgg cggcgttcat acgaacagcg gtattataaa taaagcctat 1560tatctgttgg
cacaaggcgg caccttccat ggtgtaactg tcaatgggat tggccgcgat 1620gcagcggttc
aaatttacta cagcgccttt acgaactacc tgacttcttc ttctgacttc 1680tccaatgcac
gtgatgccgt tgtacaagcg gcaaaagatc tctacggcgc gagctcggca 1740caagctaccg
cagcagccaa atcttttgat gctgtaggcg ttaac
17852595PRTPaenibacillus sp.misc_feature(1)..(595)amino acid sequence of
the PspPro3 precursor protein 2Met Leu Met Lys Lys Val Trp Val Ser
Leu Leu Gly Gly Ala Met Leu 1 5 10
15 Leu Gly Ser Val Ala Ser Gly Ala Ser Ala Ala Glu Ser Ser
Val Ser 20 25 30
Gly Pro Ala Gln Leu Thr Pro Thr Phe His Ala Glu Gln Trp Lys Ala
35 40 45 Pro Ser Ser Val
Ser Gly Asp Asp Ile Val Trp Ser Tyr Leu Asn Arg 50
55 60 Gln Lys Lys Thr Leu Leu Gly Thr
Asp Ser Thr Ser Val Arg Asp Gln 65 70
75 80 Phe Arg Ile Val Asp Arg Thr Ser Asp Lys Ser Gly
Val Ser His Tyr 85 90
95 Arg Leu Lys Gln Tyr Val Asn Gly Ile Pro Val Tyr Gly Ala Glu Gln
100 105 110 Thr Ile His
Val Gly Lys Ser Gly Glu Val Thr Ser Tyr Leu Gly Ala 115
120 125 Val Ile Thr Glu Asp Gln Gln Glu
Glu Ala Thr Gln Gly Thr Thr Pro 130 135
140 Lys Ile Ser Ala Ser Glu Ala Val His Thr Ala Tyr Gln
Glu Ala Ala 145 150 155
160 Thr Arg Val Gln Ala Leu Pro Thr Ser Asp Asp Thr Ile Ser Lys Asp
165 170 175 Ala Glu Glu Pro
Ser Ser Val Ser Lys Asp Thr Tyr Ser Glu Ala Ala 180
185 190 Asn Asn Gly Lys Thr Ser Ser Val Glu
Lys Asp Lys Leu Ser Leu Glu 195 200
205 Lys Ala Ala Asp Leu Lys Asp Ser Lys Ile Glu Ala Val Glu
Ala Glu 210 215 220
Pro Asn Ser Ile Ala Lys Ile Ala Asn Leu Gln Pro Glu Val Asp Pro 225
230 235 240 Lys Ala Glu Leu Tyr
Phe Tyr Ala Lys Gly Asp Ala Leu Gln Leu Val 245
250 255 Tyr Val Thr Glu Val Asn Ile Leu Gln Pro
Ala Pro Leu Arg Thr Arg 260 265
270 Tyr Ile Ile Asp Ala Asn Asp Gly Lys Ile Val Ser Gln Tyr Asp
Ile 275 280 285 Ile
Asn Glu Ala Thr Gly Thr Gly Lys Gly Val Leu Gly Asp Thr Lys 290
295 300 Thr Phe Asn Thr Thr Ala
Ser Gly Ser Ser Tyr Gln Leu Arg Asp Thr 305 310
315 320 Thr Arg Gly Asn Gly Ile Val Thr Tyr Thr Ala
Ser Asn Arg Gln Ser 325 330
335 Ile Pro Gly Thr Ile Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro
340 345 350 Ala Gly
Val Asp Ala His Ala Tyr Ala Ala Lys Thr Tyr Asp Tyr Tyr 355
360 365 Lys Glu Lys Phe Asn Arg Asn
Ser Ile Asp Gly Arg Gly Leu Gln Leu 370 375
380 Arg Ser Thr Val His Tyr Gly Asn Arg Tyr Asn Asn
Ala Phe Trp Asn 385 390 395
400 Gly Ser Gln Met Thr Tyr Gly Asp Gly Asp Gly Thr Thr Phe Ile Ala
405 410 415 Phe Ser Gly
Asp Pro Asp Val Val Gly His Glu Leu Thr His Gly Val 420
425 430 Thr Glu Tyr Thr Ser Asn Leu Glu
Tyr Tyr Gly Glu Ser Gly Ala Leu 435 440
445 Asn Glu Ala Phe Ser Asp Ile Ile Gly Asn Asp Ile Gln
Arg Lys Asn 450 455 460
Trp Leu Val Gly Asp Asp Ile Tyr Thr Pro Arg Ile Ala Gly Asp Ala 465
470 475 480 Leu Arg Ser Met
Ser Asn Pro Thr Leu Tyr Asp Gln Pro Asp His Tyr 485
490 495 Ser Asn Leu Tyr Arg Gly Ser Ser Asp
Asn Gly Gly Val His Thr Asn 500 505
510 Ser Gly Ile Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly
Gly Thr 515 520 525
Phe His Gly Val Thr Val Asn Gly Ile Gly Arg Asp Ala Ala Val Gln 530
535 540 Ile Tyr Tyr Ser Ala
Phe Thr Asn Tyr Leu Thr Ser Ser Ser Asp Phe 545 550
555 560 Ser Asn Ala Arg Asp Ala Val Val Gln Ala
Ala Lys Asp Leu Tyr Gly 565 570
575 Ala Ser Ser Ala Gln Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala
Val 580 585 590 Gly
Val Asn 595 3304PRTPaenibacillus sp.misc_feature(1)..(304)amino
acid sequence of the predicted mature form of PspPro3 3Ala Thr Gly
Thr Gly Lys Gly Val Leu Gly Asp Thr Lys Thr Phe Asn 1 5
10 15 Thr Thr Ala Ser Gly Ser Ser Tyr
Gln Leu Arg Asp Thr Thr Arg Gly 20 25
30 Asn Gly Ile Val Thr Tyr Thr Ala Ser Asn Arg Gln Ser
Ile Pro Gly 35 40 45
Thr Ile Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro Ala Gly Val 50
55 60 Asp Ala His Ala
Tyr Ala Ala Lys Thr Tyr Asp Tyr Tyr Lys Glu Lys 65 70
75 80 Phe Asn Arg Asn Ser Ile Asp Gly Arg
Gly Leu Gln Leu Arg Ser Thr 85 90
95 Val His Tyr Gly Asn Arg Tyr Asn Asn Ala Phe Trp Asn Gly
Ser Gln 100 105 110
Met Thr Tyr Gly Asp Gly Asp Gly Thr Thr Phe Ile Ala Phe Ser Gly
115 120 125 Asp Pro Asp Val
Val Gly His Glu Leu Thr His Gly Val Thr Glu Tyr 130
135 140 Thr Ser Asn Leu Glu Tyr Tyr Gly
Glu Ser Gly Ala Leu Asn Glu Ala 145 150
155 160 Phe Ser Asp Ile Ile Gly Asn Asp Ile Gln Arg Lys
Asn Trp Leu Val 165 170
175 Gly Asp Asp Ile Tyr Thr Pro Arg Ile Ala Gly Asp Ala Leu Arg Ser
180 185 190 Met Ser Asn
Pro Thr Leu Tyr Asp Gln Pro Asp His Tyr Ser Asn Leu 195
200 205 Tyr Arg Gly Ser Ser Asp Asn Gly
Gly Val His Thr Asn Ser Gly Ile 210 215
220 Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Thr
Phe His Gly 225 230 235
240 Val Thr Val Asn Gly Ile Gly Arg Asp Ala Ala Val Gln Ile Tyr Tyr
245 250 255 Ser Ala Phe Thr
Asn Tyr Leu Thr Ser Ser Ser Asp Phe Ser Asn Ala 260
265 270 Arg Asp Ala Val Val Gln Ala Ala Lys
Asp Leu Tyr Gly Ala Ser Ser 275 280
285 Ala Gln Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala Val Gly
Val Asn 290 295 300
41803DNAArtificial SequenceSynthetic nucleotide sequence of the
synthesized PspPro3 gene in plasmid pGX085(AprE- PspPro3) 4gtgagaagca
aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg 60gcgttcagca
acatgagcgc gcaggctgct ggaaaagcag aatcatcagt gtcaggaccg 120gctcagctta
cgccgacgtt tcatgcagag cagtggaaag caccgagcag cgttagcgga 180gatgacatcg
tgtggagcta cctgaacaga cagaagaaaa cgcttcttgg cacggacagc 240acgagcgtca
gagaccagtt cagaatcgtg gatagaacaa gcgacaaaag cggcgtcagc 300cattatagac
tgaagcagta tgtgaacgga atcccggttt atggcgcaga acaaacaatc 360catgtcggaa
agagcggcga agttacgagc tatctgggcg cggttattac agaggaccag 420caagaggagg
ctacacaagg cacgacaccg aaaatttcag catcagaggc agttcatacg 480gcctaccaag
aagctgcaac gagagttcaa gccctgccta cgtcagatga tacaatcagc 540aaagacgctg
aggaacctag ctcagttagc aaggacacgt atagcgaagc cgcgaacaat 600ggcaagacgt
caagcgtgga aaaagacaag ctttcactgg agaaggccgc tgatctgaaa 660gactcaaaga
tcgaggctgt ggaagcggaa ccgaatagca ttgcaaagat tgccaacctg 720caaccggagg
tggacccgaa ggcggagctg tatttctacg ctaaaggcga tgcactgcaa 780ctggtttacg
tcacggaggt taacatcctg cagccggcac cgcttagaac gagatacatc 840attgacgcga
acgacggcaa gatcgtgagc cagtacgaca ttatcaacga ggccacggga 900acgggcaagg
gagtccttgg cgacacgaag acattcaata caacggcctc aggctcatca 960taccagctga
gagacacgac gagaggcaac ggaatcgtca cgtacacggc tagcaataga 1020cagagcattc
cgggcacaat ccttacggac gcagacaatg tgtggaatga cccggcaggc 1080gtggacgcac
atgcctacgc agcgaagacg tacgactact acaaggagaa gttcaacaga 1140aacagcatcg
acggaagagg actgcaactt agaagcacgg tgcattacgg caacagatac 1200aacaacgctt
tctggaacgg cagccaaatg acgtatggag acggcgatgg aacaacgttt 1260atcgcattct
caggcgaccc tgacgttgtg ggacatgaac tgacgcatgg agtcacagaa 1320tacacgagca
atctggagta ttacggagaa tcaggcgcac ttaatgaggc cttcagcgac 1380atcatcggaa
acgacatcca gagaaagaac tggctggttg gcgatgatat ctacacgccg 1440agaattgcgg
gcgacgcgct gagatcaatg agcaacccta cgctgtacga tcagccggat 1500cattacagca
acctgtatag aggctcaagc gataatggcg gcgtgcatac aaacagcggc 1560atcatcaaca
aagcctatta tctgctggcg caaggcggca cattccatgg cgttacagtt 1620aatggcattg
gcagagacgc agccgtgcag atctactaca gcgcattcac gaattacctg 1680acatcaagca
gcgacttttc aaatgcaaga gatgcagtgg tgcaggcggc taaagacctt 1740tatggagctt
caagcgctca ggccacagct gcggcaaaaa gcttcgacgc ggttggagtg 1800aat
18035601PRTArtificial SequenceSynthetic amino acid sequence of the
PspPro3 precursor protein expressed from plasmid pGX085(AprE-
PspPro3) 5Met Arg Ser Lys Lys Leu Trp Ile Ser Leu Leu Phe Ala Leu Thr Leu
1 5 10 15 Ile Phe
Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala Ala Gly Lys 20
25 30 Ala Glu Ser Ser Val Ser Gly
Pro Ala Gln Leu Thr Pro Thr Phe His 35 40
45 Ala Glu Gln Trp Lys Ala Pro Ser Ser Val Ser Gly
Asp Asp Ile Val 50 55 60
Trp Ser Tyr Leu Asn Arg Gln Lys Lys Thr Leu Leu Gly Thr Asp Ser 65
70 75 80 Thr Ser Val
Arg Asp Gln Phe Arg Ile Val Asp Arg Thr Ser Asp Lys 85
90 95 Ser Gly Val Ser His Tyr Arg Leu
Lys Gln Tyr Val Asn Gly Ile Pro 100 105
110 Val Tyr Gly Ala Glu Gln Thr Ile His Val Gly Lys Ser
Gly Glu Val 115 120 125
Thr Ser Tyr Leu Gly Ala Val Ile Thr Glu Asp Gln Gln Glu Glu Ala 130
135 140 Thr Gln Gly Thr
Thr Pro Lys Ile Ser Ala Ser Glu Ala Val His Thr 145 150
155 160 Ala Tyr Gln Glu Ala Ala Thr Arg Val
Gln Ala Leu Pro Thr Ser Asp 165 170
175 Asp Thr Ile Ser Lys Asp Ala Glu Glu Pro Ser Ser Val Ser
Lys Asp 180 185 190
Thr Tyr Ser Glu Ala Ala Asn Asn Gly Lys Thr Ser Ser Val Glu Lys
195 200 205 Asp Lys Leu Ser
Leu Glu Lys Ala Ala Asp Leu Lys Asp Ser Lys Ile 210
215 220 Glu Ala Val Glu Ala Glu Pro Asn
Ser Ile Ala Lys Ile Ala Asn Leu 225 230
235 240 Gln Pro Glu Val Asp Pro Lys Ala Glu Leu Tyr Phe
Tyr Ala Lys Gly 245 250
255 Asp Ala Leu Gln Leu Val Tyr Val Thr Glu Val Asn Ile Leu Gln Pro
260 265 270 Ala Pro Leu
Arg Thr Arg Tyr Ile Ile Asp Ala Asn Asp Gly Lys Ile 275
280 285 Val Ser Gln Tyr Asp Ile Ile Asn
Glu Ala Thr Gly Thr Gly Lys Gly 290 295
300 Val Leu Gly Asp Thr Lys Thr Phe Asn Thr Thr Ala Ser
Gly Ser Ser 305 310 315
320 Tyr Gln Leu Arg Asp Thr Thr Arg Gly Asn Gly Ile Val Thr Tyr Thr
325 330 335 Ala Ser Asn Arg
Gln Ser Ile Pro Gly Thr Ile Leu Thr Asp Ala Asp 340
345 350 Asn Val Trp Asn Asp Pro Ala Gly Val
Asp Ala His Ala Tyr Ala Ala 355 360
365 Lys Thr Tyr Asp Tyr Tyr Lys Glu Lys Phe Asn Arg Asn Ser
Ile Asp 370 375 380
Gly Arg Gly Leu Gln Leu Arg Ser Thr Val His Tyr Gly Asn Arg Tyr 385
390 395 400 Asn Asn Ala Phe Trp
Asn Gly Ser Gln Met Thr Tyr Gly Asp Gly Asp 405
410 415 Gly Thr Thr Phe Ile Ala Phe Ser Gly Asp
Pro Asp Val Val Gly His 420 425
430 Glu Leu Thr His Gly Val Thr Glu Tyr Thr Ser Asn Leu Glu Tyr
Tyr 435 440 445 Gly
Glu Ser Gly Ala Leu Asn Glu Ala Phe Ser Asp Ile Ile Gly Asn 450
455 460 Asp Ile Gln Arg Lys Asn
Trp Leu Val Gly Asp Asp Ile Tyr Thr Pro 465 470
475 480 Arg Ile Ala Gly Asp Ala Leu Arg Ser Met Ser
Asn Pro Thr Leu Tyr 485 490
495 Asp Gln Pro Asp His Tyr Ser Asn Leu Tyr Arg Gly Ser Ser Asp Asn
500 505 510 Gly Gly
Val His Thr Asn Ser Gly Ile Ile Asn Lys Ala Tyr Tyr Leu 515
520 525 Leu Ala Gln Gly Gly Thr Phe
His Gly Val Thr Val Asn Gly Ile Gly 530 535
540 Arg Asp Ala Ala Val Gln Ile Tyr Tyr Ser Ala Phe
Thr Asn Tyr Leu 545 550 555
560 Thr Ser Ser Ser Asp Phe Ser Asn Ala Arg Asp Ala Val Val Gln Ala
565 570 575 Ala Lys Asp
Leu Tyr Gly Ala Ser Ser Ala Gln Ala Thr Ala Ala Ala 580
585 590 Lys Ser Phe Asp Ala Val Gly Val
Asn 595 600 61770DNAPaenibacillus
sp.misc_feature(1)..(1770)nucleotide sequence of the PspPro2 gene
isolated from Paenibacillus sp. 6atgaaaaaag tatgggtttc acttcttgga
ggagcgatgt tattaggggc tgtagcacca 60ggtgcatcag cagcagagca ttctgttcct
gatcctactc agctaacacc gacctttcac 120gccgagcaat ggaaggctcc ttccacggta
accggcgaca atattgtatg gagctatttg 180aatcgacaaa agaaaacctt attgaataca
gacagcacca gtgtgcgtga tcagttccgc 240atcattgatc gtacaagcga caaatccggt
gcaagccatt atcggctcaa gcaatatgta 300aacgggatcc ccgtatatgg ggctgaacag
accattcatg tgaacaacgc cggtaaagta 360acctcttatt tgggtgctgt catttcagag
gatcagcagc aagacgcgac cgaagatacc 420actccaaaaa tcagcgcgac tgaagccgtt
tataccgcat atgcagaagc cgctgcccgg 480attcaatcct tcccttccat caatgatagt
ctttctgagg ctagtgagga acaagggagt 540gagaatcaag gcaatgagat tcaaaacatt
gggattaaaa gcagtgtaag taatgacact 600tacgcagagg cgcataacaa cgtactttta
acccccgttg accaagcaga gcaaagttac 660attgccaaaa ttgctaatct ggagccaagt
gtagagccca aagcagaatt atacatctat 720ccagatggtg agactacacg actggtttat
gtaacagagg ttaatattct tgaacctgcg 780cctctgcgca cacgctactt cattgatgcg
aaaaccggca aaatcgtatt ccagtatgac 840atcctcaacc acgcaacagg caccggccgc
ggcgtggatg gcaaaacaaa atcatttacg 900actacagctt caggcaaccg gtatcagttg
aaagacacga ctcgcagcaa tggaatcgtg 960acttacaccg ctggcaatcg ccagacgacg
ccaggtacga ttttgaccga tacagataat 1020gtatgggagg accctgcggc tgttgatgcc
catgcctacg ccattaaaac ctatgactat 1080tataagaata aattcggtcg cgacagtatt
gatggacgtg gcatgcaaat tcgttcgaca 1140gtccattacg gcaaaaaata taacaatgcc
ttctggaacg gctcgcaaat gacctacgga 1200gacggagacg ggtccacatt taccttcttc
agcggcgatc ccgatgtcgt ggggcatgag 1260ctcacccacg gcgtcaccga gttcacctcc
aatttggagt attatggtga gtccggtgca 1320ttgaacgaag ccttctcgga tattatcggt
aatgatatag atggcaccag ttggcttctt 1380ggcgacggca tttatacgcc taatattcca
ggcgacgctc tgcgttccct gtccgatcct 1440acacgattcg gccagccgga tcactactcc
aatttctatc cggaccccaa caatgatgat 1500gaaggcggag tccatacgaa cagcggtatt
atcaacaaag cctattattt gctggcacaa 1560ggcggtacgt cccatggtgt aacggtaact
ggtatcggac gcgaagcggc tgtattcatt 1620tactacaatg cctttaccaa ctatttgacc
tctacctcca acttctctaa cgcacgcgct 1680gctgttatac aggcagccaa ggatttttat
ggtgctgatt cgctggcagt aaccagtgct 1740attcaatcct ttgatgcggt aggaatcaaa
17707590PRTPaenibacillus
sp.misc_feature(1)..(590)amino acid sequence of the PspPro2 precursor
protein 7Met Lys Lys Val Trp Val Ser Leu Leu Gly Gly Ala Met Leu Leu Gly
1 5 10 15 Ala Val
Ala Pro Gly Ala Ser Ala Ala Glu His Ser Val Pro Asp Pro 20
25 30 Thr Gln Leu Thr Pro Thr Phe
His Ala Glu Gln Trp Lys Ala Pro Ser 35 40
45 Thr Val Thr Gly Asp Asn Ile Val Trp Ser Tyr Leu
Asn Arg Gln Lys 50 55 60
Lys Thr Leu Leu Asn Thr Asp Ser Thr Ser Val Arg Asp Gln Phe Arg 65
70 75 80 Ile Ile Asp
Arg Thr Ser Asp Lys Ser Gly Ala Ser His Tyr Arg Leu 85
90 95 Lys Gln Tyr Val Asn Gly Ile Pro
Val Tyr Gly Ala Glu Gln Thr Ile 100 105
110 His Val Asn Asn Ala Gly Lys Val Thr Ser Tyr Leu Gly
Ala Val Ile 115 120 125
Ser Glu Asp Gln Gln Gln Asp Ala Thr Glu Asp Thr Thr Pro Lys Ile 130
135 140 Ser Ala Thr Glu
Ala Val Tyr Thr Ala Tyr Ala Glu Ala Ala Ala Arg 145 150
155 160 Ile Gln Ser Phe Pro Ser Ile Asn Asp
Ser Leu Ser Glu Ala Ser Glu 165 170
175 Glu Gln Gly Ser Glu Asn Gln Gly Asn Glu Ile Gln Asn Ile
Gly Ile 180 185 190
Lys Ser Ser Val Ser Asn Asp Thr Tyr Ala Glu Ala His Asn Asn Val
195 200 205 Leu Leu Thr Pro
Val Asp Gln Ala Glu Gln Ser Tyr Ile Ala Lys Ile 210
215 220 Ala Asn Leu Glu Pro Ser Val Glu
Pro Lys Ala Glu Leu Tyr Ile Tyr 225 230
235 240 Pro Asp Gly Glu Thr Thr Arg Leu Val Tyr Val Thr
Glu Val Asn Ile 245 250
255 Leu Glu Pro Ala Pro Leu Arg Thr Arg Tyr Phe Ile Asp Ala Lys Thr
260 265 270 Gly Lys Ile
Val Phe Gln Tyr Asp Ile Leu Asn His Ala Thr Gly Thr 275
280 285 Gly Arg Gly Val Asp Gly Lys Thr
Lys Ser Phe Thr Thr Thr Ala Ser 290 295
300 Gly Asn Arg Tyr Gln Leu Lys Asp Thr Thr Arg Ser Asn
Gly Ile Val 305 310 315
320 Thr Tyr Thr Ala Gly Asn Arg Gln Thr Thr Pro Gly Thr Ile Leu Thr
325 330 335 Asp Thr Asp Asn
Val Trp Glu Asp Pro Ala Ala Val Asp Ala His Ala 340
345 350 Tyr Ala Ile Lys Thr Tyr Asp Tyr Tyr
Lys Asn Lys Phe Gly Arg Asp 355 360
365 Ser Ile Asp Gly Arg Gly Met Gln Ile Arg Ser Thr Val His
Tyr Gly 370 375 380
Lys Lys Tyr Asn Asn Ala Phe Trp Asn Gly Ser Gln Met Thr Tyr Gly 385
390 395 400 Asp Gly Asp Gly Ser
Thr Phe Thr Phe Phe Ser Gly Asp Pro Asp Val 405
410 415 Val Gly His Glu Leu Thr His Gly Val Thr
Glu Phe Thr Ser Asn Leu 420 425
430 Glu Tyr Tyr Gly Glu Ser Gly Ala Leu Asn Glu Ala Phe Ser Asp
Ile 435 440 445 Ile
Gly Asn Asp Ile Asp Gly Thr Ser Trp Leu Leu Gly Asp Gly Ile 450
455 460 Tyr Thr Pro Asn Ile Pro
Gly Asp Ala Leu Arg Ser Leu Ser Asp Pro 465 470
475 480 Thr Arg Phe Gly Gln Pro Asp His Tyr Ser Asn
Phe Tyr Pro Asp Pro 485 490
495 Asn Asn Asp Asp Glu Gly Gly Val His Thr Asn Ser Gly Ile Ile Asn
500 505 510 Lys Ala
Tyr Tyr Leu Leu Ala Gln Gly Gly Thr Ser His Gly Val Thr 515
520 525 Val Thr Gly Ile Gly Arg Glu
Ala Ala Val Phe Ile Tyr Tyr Asn Ala 530 535
540 Phe Thr Asn Tyr Leu Thr Ser Thr Ser Asn Phe Ser
Asn Ala Arg Ala 545 550 555
560 Ala Val Ile Gln Ala Ala Lys Asp Phe Tyr Gly Ala Asp Ser Leu Ala
565 570 575 Val Thr Ser
Ala Ile Gln Ser Phe Asp Ala Val Gly Ile Lys 580
585 590 8306PRTPaenibacillus
sp.misc_feature(1)..(306)amino acid sequence of the predicted mature
form of PspPro2 8Ala Thr Gly Thr Gly Arg Gly Val Asp Gly Lys Thr Lys Ser
Phe Thr 1 5 10 15
Thr Thr Ala Ser Gly Asn Arg Tyr Gln Leu Lys Asp Thr Thr Arg Ser
20 25 30 Asn Gly Ile Val Thr
Tyr Thr Ala Gly Asn Arg Gln Thr Thr Pro Gly 35
40 45 Thr Ile Leu Thr Asp Thr Asp Asn Val
Trp Glu Asp Pro Ala Ala Val 50 55
60 Asp Ala His Ala Tyr Ala Ile Lys Thr Tyr Asp Tyr Tyr
Lys Asn Lys 65 70 75
80 Phe Gly Arg Asp Ser Ile Asp Gly Arg Gly Met Gln Ile Arg Ser Thr
85 90 95 Val His Tyr Gly
Lys Lys Tyr Asn Asn Ala Phe Trp Asn Gly Ser Gln 100
105 110 Met Thr Tyr Gly Asp Gly Asp Gly Ser
Thr Phe Thr Phe Phe Ser Gly 115 120
125 Asp Pro Asp Val Val Gly His Glu Leu Thr His Gly Val Thr
Glu Phe 130 135 140
Thr Ser Asn Leu Glu Tyr Tyr Gly Glu Ser Gly Ala Leu Asn Glu Ala 145
150 155 160 Phe Ser Asp Ile Ile
Gly Asn Asp Ile Asp Gly Thr Ser Trp Leu Leu 165
170 175 Gly Asp Gly Ile Tyr Thr Pro Asn Ile Pro
Gly Asp Ala Leu Arg Ser 180 185
190 Leu Ser Asp Pro Thr Arg Phe Gly Gln Pro Asp His Tyr Ser Asn
Phe 195 200 205 Tyr
Pro Asp Pro Asn Asn Asp Asp Glu Gly Gly Val His Thr Asn Ser 210
215 220 Gly Ile Ile Asn Lys Ala
Tyr Tyr Leu Leu Ala Gln Gly Gly Thr Ser 225 230
235 240 His Gly Val Thr Val Thr Gly Ile Gly Arg Glu
Ala Ala Val Phe Ile 245 250
255 Tyr Tyr Asn Ala Phe Thr Asn Tyr Leu Thr Ser Thr Ser Asn Phe Ser
260 265 270 Asn Ala
Arg Ala Ala Val Ile Gln Ala Ala Lys Asp Phe Tyr Gly Ala 275
280 285 Asp Ser Leu Ala Val Thr Ser
Ala Ile Gln Ser Phe Asp Ala Val Gly 290 295
300 Ile Lys 305 91794DNAArtificial
SequenceSynthetic nucleotide sequence of the synthesized PspPro2
gene in plasmid pGX084 (AprE-PspPro2) 9gtgagaagca aaaaattgtg gatcagcttg
ttgtttgcgt taacgttaat ctttacgatg 60gcgttcagca acatgagcgc gcaggctgct
ggaaaagcag agcattcagt tcctgacccg 120acgcaactta caccgacatt tcatgctgag
cagtggaagg caccgagcac ggtcacgggc 180gacaacatcg tgtggagcta cctgaacaga
cagaaaaaga cgctgctgaa cacggactca 240acgagcgtga gagaccagtt cagaatcatc
gacagaacga gcgacaagtc aggcgcgtca 300cattatagac tgaagcagta cgtgaacggc
atcccggtct acggagccga gcaaacgatc 360catgtgaata atgcgggcaa agttacatca
tacctgggcg ccgtcatctc agaagaccag 420cagcaagatg caacggagga tacaacaccg
aagatcagcg ccacagaagc ggtctatacg 480gcttacgccg aagcggctgc aagaatccag
agcttcccgt caattaatga cagcctgagc 540gaagcatcag aggaacaagg cagcgagaac
cagggcaatg aaatccaaaa catcggcatc 600aagagcagcg tgtcaaacga cacgtatgcg
gaggctcata acaacgttct gctgacaccg 660gtcgatcagg ccgaacagag ctatattgca
aagatcgcga atctggagcc gtcagtcgag 720ccgaaggccg agctgtatat ctatccggac
ggcgagacga cgagactggt gtacgttacg 780gaggtcaaca tccttgagcc tgcgccgctg
agaacaagat actttatcga cgccaagacg 840ggcaagatcg tgtttcagta cgatatcctg
aaccatgcga cgggaacagg cagaggcgtg 900gacggcaaaa caaaatcatt cacgacaacg
gcaagcggca acagatacca gctgaaggac 960acaacaagat caaatggcat cgtcacatac
acggccggaa atagacagac gacgccggga 1020acgattctga cggatacaga taacgtgtgg
gaagatccgg cagcagttga tgcacatgca 1080tacgcgatca agacgtacga ctactacaag
aacaaattcg gaagagattc aatcgatgga 1140agaggcatgc aaatcagatc aacggttcat
tatggcaaaa agtacaacaa tgccttctgg 1200aacggcagcc aaatgacata cggcgatgga
gacggctcaa cgtttacatt cttttcaggc 1260gacccggacg tcgtcggcca tgaactgacg
catggcgtta cagagttcac gagcaacctg 1320gagtattacg gcgaatcagg cgcactgaat
gaggctttca gcgacatcat tggcaacgac 1380attgatggca catcatggct gcttggcgac
ggcatttaca cacctaacat tccgggcgat 1440gcactgagaa gcctgtcaga ccctacgaga
ttcggccaac ctgaccatta cagcaacttc 1500tacccggatc ctaataacga tgatgagggc
ggagtgcata cgaacagcgg cattatcaac 1560aaagcgtact atctgctggc acaaggcgga
acgtcacatg gagtgacggt gacaggaatc 1620ggcagagagg cggcagtgtt tatctactac
aacgccttca caaactacct gacgagcacg 1680tcaaatttca gcaacgctag agcggcggtc
atccaggcag caaaggactt ttatggagca 1740gactcactgg cagttacgtc agcaattcag
tcattcgacg cagttggaat taag 179410598PRTArtificial
SequenceSynthetic amino acid sequence of the PspPro2 precursor
protein expressed from plasmid pGX084(AprE-PspPro2) 10Met Arg Ser Lys Lys
Leu Trp Ile Ser Leu Leu Phe Ala Leu Thr Leu 1 5
10 15 Ile Phe Thr Met Ala Phe Ser Asn Met Ser
Ala Gln Ala Ala Gly Lys 20 25
30 Ala Glu His Ser Val Pro Asp Pro Thr Gln Leu Thr Pro Thr Phe
His 35 40 45 Ala
Glu Gln Trp Lys Ala Pro Ser Thr Val Thr Gly Asp Asn Ile Val 50
55 60 Trp Ser Tyr Leu Asn Arg
Gln Lys Lys Thr Leu Leu Asn Thr Asp Ser 65 70
75 80 Thr Ser Val Arg Asp Gln Phe Arg Ile Ile Asp
Arg Thr Ser Asp Lys 85 90
95 Ser Gly Ala Ser His Tyr Arg Leu Lys Gln Tyr Val Asn Gly Ile Pro
100 105 110 Val Tyr
Gly Ala Glu Gln Thr Ile His Val Asn Asn Ala Gly Lys Val 115
120 125 Thr Ser Tyr Leu Gly Ala Val
Ile Ser Glu Asp Gln Gln Gln Asp Ala 130 135
140 Thr Glu Asp Thr Thr Pro Lys Ile Ser Ala Thr Glu
Ala Val Tyr Thr 145 150 155
160 Ala Tyr Ala Glu Ala Ala Ala Arg Ile Gln Ser Phe Pro Ser Ile Asn
165 170 175 Asp Ser Leu
Ser Glu Ala Ser Glu Glu Gln Gly Ser Glu Asn Gln Gly 180
185 190 Asn Glu Ile Gln Asn Ile Gly Ile
Lys Ser Ser Val Ser Asn Asp Thr 195 200
205 Tyr Ala Glu Ala His Asn Asn Val Leu Leu Thr Pro Val
Asp Gln Ala 210 215 220
Glu Gln Ser Tyr Ile Ala Lys Ile Ala Asn Leu Glu Pro Ser Val Glu 225
230 235 240 Pro Lys Ala Glu
Leu Tyr Ile Tyr Pro Asp Gly Glu Thr Thr Arg Leu 245
250 255 Val Tyr Val Thr Glu Val Asn Ile Leu
Glu Pro Ala Pro Leu Arg Thr 260 265
270 Arg Tyr Phe Ile Asp Ala Lys Thr Gly Lys Ile Val Phe Gln
Tyr Asp 275 280 285
Ile Leu Asn His Ala Thr Gly Thr Gly Arg Gly Val Asp Gly Lys Thr 290
295 300 Lys Ser Phe Thr Thr
Thr Ala Ser Gly Asn Arg Tyr Gln Leu Lys Asp 305 310
315 320 Thr Thr Arg Ser Asn Gly Ile Val Thr Tyr
Thr Ala Gly Asn Arg Gln 325 330
335 Thr Thr Pro Gly Thr Ile Leu Thr Asp Thr Asp Asn Val Trp Glu
Asp 340 345 350 Pro
Ala Ala Val Asp Ala His Ala Tyr Ala Ile Lys Thr Tyr Asp Tyr 355
360 365 Tyr Lys Asn Lys Phe Gly
Arg Asp Ser Ile Asp Gly Arg Gly Met Gln 370 375
380 Ile Arg Ser Thr Val His Tyr Gly Lys Lys Tyr
Asn Asn Ala Phe Trp 385 390 395
400 Asn Gly Ser Gln Met Thr Tyr Gly Asp Gly Asp Gly Ser Thr Phe Thr
405 410 415 Phe Phe
Ser Gly Asp Pro Asp Val Val Gly His Glu Leu Thr His Gly 420
425 430 Val Thr Glu Phe Thr Ser Asn
Leu Glu Tyr Tyr Gly Glu Ser Gly Ala 435 440
445 Leu Asn Glu Ala Phe Ser Asp Ile Ile Gly Asn Asp
Ile Asp Gly Thr 450 455 460
Ser Trp Leu Leu Gly Asp Gly Ile Tyr Thr Pro Asn Ile Pro Gly Asp 465
470 475 480 Ala Leu Arg
Ser Leu Ser Asp Pro Thr Arg Phe Gly Gln Pro Asp His 485
490 495 Tyr Ser Asn Phe Tyr Pro Asp Pro
Asn Asn Asp Asp Glu Gly Gly Val 500 505
510 His Thr Asn Ser Gly Ile Ile Asn Lys Ala Tyr Tyr Leu
Leu Ala Gln 515 520 525
Gly Gly Thr Ser His Gly Val Thr Val Thr Gly Ile Gly Arg Glu Ala 530
535 540 Ala Val Phe Ile
Tyr Tyr Asn Ala Phe Thr Asn Tyr Leu Thr Ser Thr 545 550
555 560 Ser Asn Phe Ser Asn Ala Arg Ala Ala
Val Ile Gln Ala Ala Lys Asp 565 570
575 Phe Tyr Gly Ala Asp Ser Leu Ala Val Thr Ser Ala Ile Gln
Ser Phe 580 585 590
Asp Ala Val Gly Ile Lys 595 111599DNAPaenibacillus
humicusmisc_feature(1)..(1599)nucleotide sequence of the PhuPro2 gene
isolated from Paenibacillus humicus 11atgaaaaaaa tgattcctac tctgctcggt
accgtattgc tgctttcttc cgcttccgct 60gtcgctgctg aatcgccaag cctcggagcg
gccggaactc ccggggtcag cgtcgtgaac 120aatcagctcg tgactcaatt catcgaggct
tccaaggatg ccaagattgt cccgggctct 180tccgaggata aaatctgggc tttccttgaa
ggccagcaag caaagctggg tgtatccgca 240gcggatgtaa aaacctcgtt cctgatccag
aagaaggaag tcgatccgac ttcgggcgtc 300gagcatttcc gcctgcagca atatgtgaat
ggcatcccgg tatatggcgg tgaccaaacc 360attcacatcg acaaggccgg ccaggttacg
tcgttcgtag gagctgttct gccggctcaa 420aatcaaatca cggcaaaatc cagcgtacca
gccataagcg catccgacgc tctggctatc 480gcggcgaagg aagccagttc ccgcatcggc
gagctgggag cacaggagaa gactccgtcg 540gctcagctgt acgtatatcc ggaaggcaac
gggtcgcgtc tcgtctacca gacggaagtg 600aatgtgcttg agccgcagcc tctgcgcacc
cgctatctta tcgatgcggc cgacggccat 660atcgtgcagc agtacgatct gatcgagacg
gcgaccggtt cgggcacggg cgtgctgggc 720gacaataaga cgttccagac gactctttcc
ggcagcacgt accagctgaa agacaccact 780cgcggcaacg gcatctacac ctacacagcc
agcaatcgga ccacgattcc gggcacgctg 840ctgacggacg ccgacaacgt atggacggat
ggagccgccg tcgatgccca tacttatgcc 900ggaaaagtat atgatttcta caaaacgaag
ttcggacgca acagcctcga cggcaacggc 960ctgctgatcc gttcctcggt ccactacagc
agcaggtaca acaatgcctt ctggaacggc 1020acccagattg tattcggcga cggcgacggc
tcgacgttca ttccgctgtc gggcgatctc 1080gacgtggtcg gccatgagct gtcccacgga
gtcatcgagt acacgtccaa ccttcaatac 1140ctcaatgaat ccggcgcgct gaacgagtcc
tatgccgacg tcctcggcaa ctcgatccag 1200gcgaaaaact ggcttatcgg cgacgatgtc
tatacgcctg gcatctccgg agatgctctc 1260cgttccatgt ccaacccgac gctttacggg
cagccggaca actatgccaa ccgctatacg 1320ggatcttccg acaacggcgg cgttcatacg
aacagcggca tcacgaacaa agcgttctac 1380ctgctcgccc aaggcggcac ccagaacggc
gttaccgtcg ccggcatcgg gcgcgacgca 1440gccgtgaaca ttttctacaa cacagtggcc
tattacctta cttccacttc caacttcgcc 1500gcggcgaaga acgcctcgat ccaggcagcc
aaagacctgt acggaacggg ctcctcttat 1560gtcacctcgg tgaccaatgc attcagagcc
gtaggcctg 159912533PRTPaenibacillus
humicusmisc_feature(1)..(533)amino acid sequence of the PhuPro2 precursor
protein 12Met Lys Lys Met Ile Pro Thr Leu Leu Gly Thr Val Leu Leu
Leu Ser 1 5 10 15
Ser Ala Ser Ala Val Ala Ala Glu Ser Pro Ser Leu Gly Ala Ala Gly
20 25 30 Thr Pro Gly Val Ser
Val Val Asn Asn Gln Leu Val Thr Gln Phe Ile 35
40 45 Glu Ala Ser Lys Asp Ala Lys Ile Val
Pro Gly Ser Ser Glu Asp Lys 50 55
60 Ile Trp Ala Phe Leu Glu Gly Gln Gln Ala Lys Leu Gly
Val Ser Ala 65 70 75
80 Ala Asp Val Lys Thr Ser Phe Leu Ile Gln Lys Lys Glu Val Asp Pro
85 90 95 Thr Ser Gly Val
Glu His Phe Arg Leu Gln Gln Tyr Val Asn Gly Ile 100
105 110 Pro Val Tyr Gly Gly Asp Gln Thr Ile
His Ile Asp Lys Ala Gly Gln 115 120
125 Val Thr Ser Phe Val Gly Ala Val Leu Pro Ala Gln Asn Gln
Ile Thr 130 135 140
Ala Lys Ser Ser Val Pro Ala Ile Ser Ala Ser Asp Ala Leu Ala Ile 145
150 155 160 Ala Ala Lys Glu Ala
Ser Ser Arg Ile Gly Glu Leu Gly Ala Gln Glu 165
170 175 Lys Thr Pro Ser Ala Gln Leu Tyr Val Tyr
Pro Glu Gly Asn Gly Ser 180 185
190 Arg Leu Val Tyr Gln Thr Glu Val Asn Val Leu Glu Pro Gln Pro
Leu 195 200 205 Arg
Thr Arg Tyr Leu Ile Asp Ala Ala Asp Gly His Ile Val Gln Gln 210
215 220 Tyr Asp Leu Ile Glu Thr
Ala Thr Gly Ser Gly Thr Gly Val Leu Gly 225 230
235 240 Asp Asn Lys Thr Phe Gln Thr Thr Leu Ser Gly
Ser Thr Tyr Gln Leu 245 250
255 Lys Asp Thr Thr Arg Gly Asn Gly Ile Tyr Thr Tyr Thr Ala Ser Asn
260 265 270 Arg Thr
Thr Ile Pro Gly Thr Leu Leu Thr Asp Ala Asp Asn Val Trp 275
280 285 Thr Asp Gly Ala Ala Val Asp
Ala His Thr Tyr Ala Gly Lys Val Tyr 290 295
300 Asp Phe Tyr Lys Thr Lys Phe Gly Arg Asn Ser Leu
Asp Gly Asn Gly 305 310 315
320 Leu Leu Ile Arg Ser Ser Val His Tyr Ser Ser Arg Tyr Asn Asn Ala
325 330 335 Phe Trp Asn
Gly Thr Gln Ile Val Phe Gly Asp Gly Asp Gly Ser Thr 340
345 350 Phe Ile Pro Leu Ser Gly Asp Leu
Asp Val Val Gly His Glu Leu Ser 355 360
365 His Gly Val Ile Glu Tyr Thr Ser Asn Leu Gln Tyr Leu
Asn Glu Ser 370 375 380
Gly Ala Leu Asn Glu Ser Tyr Ala Asp Val Leu Gly Asn Ser Ile Gln 385
390 395 400 Ala Lys Asn Trp
Leu Ile Gly Asp Asp Val Tyr Thr Pro Gly Ile Ser 405
410 415 Gly Asp Ala Leu Arg Ser Met Ser Asn
Pro Thr Leu Tyr Gly Gln Pro 420 425
430 Asp Asn Tyr Ala Asn Arg Tyr Thr Gly Ser Ser Asp Asn Gly
Gly Val 435 440 445
His Thr Asn Ser Gly Ile Thr Asn Lys Ala Phe Tyr Leu Leu Ala Gln 450
455 460 Gly Gly Thr Gln Asn
Gly Val Thr Val Ala Gly Ile Gly Arg Asp Ala 465 470
475 480 Ala Val Asn Ile Phe Tyr Asn Thr Val Ala
Tyr Tyr Leu Thr Ser Thr 485 490
495 Ser Asn Phe Ala Ala Ala Lys Asn Ala Ser Ile Gln Ala Ala Lys
Asp 500 505 510 Leu
Tyr Gly Thr Gly Ser Ser Tyr Val Thr Ser Val Thr Asn Ala Phe 515
520 525 Arg Ala Val Gly Leu
530 13303PRTPaenibacillus humicusmisc_feature(1)..(303)amino
acid sequence of the predicted mature form of PhuPro2 13Ala Thr Gly
Ser Gly Thr Gly Val Leu Gly Asp Asn Lys Thr Phe Gln 1 5
10 15 Thr Thr Leu Ser Gly Ser Thr Tyr
Gln Leu Lys Asp Thr Thr Arg Gly 20 25
30 Asn Gly Ile Tyr Thr Tyr Thr Ala Ser Asn Arg Thr Thr
Ile Pro Gly 35 40 45
Thr Leu Leu Thr Asp Ala Asp Asn Val Trp Thr Asp Gly Ala Ala Val 50
55 60 Asp Ala His Thr
Tyr Ala Gly Lys Val Tyr Asp Phe Tyr Lys Thr Lys 65 70
75 80 Phe Gly Arg Asn Ser Leu Asp Gly Asn
Gly Leu Leu Ile Arg Ser Ser 85 90
95 Val His Tyr Ser Ser Arg Tyr Asn Asn Ala Phe Trp Asn Gly
Thr Gln 100 105 110
Ile Val Phe Gly Asp Gly Asp Gly Ser Thr Phe Ile Pro Leu Ser Gly
115 120 125 Asp Leu Asp Val
Val Gly His Glu Leu Ser His Gly Val Ile Glu Tyr 130
135 140 Thr Ser Asn Leu Gln Tyr Leu Asn
Glu Ser Gly Ala Leu Asn Glu Ser 145 150
155 160 Tyr Ala Asp Val Leu Gly Asn Ser Ile Gln Ala Lys
Asn Trp Leu Ile 165 170
175 Gly Asp Asp Val Tyr Thr Pro Gly Ile Ser Gly Asp Ala Leu Arg Ser
180 185 190 Met Ser Asn
Pro Thr Leu Tyr Gly Gln Pro Asp Asn Tyr Ala Asn Arg 195
200 205 Tyr Thr Gly Ser Ser Asp Asn Gly
Gly Val His Thr Asn Ser Gly Ile 210 215
220 Thr Asn Lys Ala Phe Tyr Leu Leu Ala Gln Gly Gly Thr
Gln Asn Gly 225 230 235
240 Val Thr Val Ala Gly Ile Gly Arg Asp Ala Ala Val Asn Ile Phe Tyr
245 250 255 Asn Thr Val Ala
Tyr Tyr Leu Thr Ser Thr Ser Asn Phe Ala Ala Ala 260
265 270 Lys Asn Ala Ser Ile Gln Ala Ala Lys
Asp Leu Tyr Gly Thr Gly Ser 275 280
285 Ser Tyr Val Thr Ser Val Thr Asn Ala Phe Arg Ala Val Gly
Leu 290 295 300
141629DNAArtificial SequenceSynthetic nucleotide sequence of the
synthesized PhuPro2 gene in plasmid pGX150(AprE- PhuPro2) 14gtgagaagca
aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg 60gcgttcagca
acatgagcgc gcaggctgct ggaaaagaat caccgagcct tggcgctgca 120ggaacaccgg
gcgttagcgt tgtgaataac caactggtca cgcagttcat cgaagcatca 180aaagacgcga
aaattgtccc tggatcaagc gaagataaga tttgggcatt tctggaaggc 240cagcaagcaa
agcttggcgt ctcagctgcc gacgtgaaga cgagcttcct gatccagaag 300aaggaggttg
acccgacatc aggcgttgag cactttagac tgcaacagta cgtcaacggc 360atcccggttt
atggaggcga tcaaacaatc catattgata aggcaggcca ggtcacatca 420ttcgtcggag
ctgtcctgcc ggctcagaac caaattacag caaaatcatc agttccggca 480atttcagcct
cagacgctct ggcaatcgct gccaaggagg caagctcaag aattggcgaa 540ctgggcgcac
aagaaaagac accgagcgcc caactttatg tctatccgga gggcaacgga 600agcagactgg
tgtaccagac agaggtcaat gttctggagc cgcaaccgct gagaacgaga 660taccttatcg
atgctgcgga tggccacatt gttcagcaat acgacctgat tgagacagca 720acaggaagcg
gaacgggcgt gctgggcgac aacaagacgt ttcagacaac acttagcggc 780agcacgtacc
aacttaagga cacgacgaga ggcaatggca tttacacgta cacggcctca 840aacagaacga
caatcccagg cacactgctg acggatgcag acaatgtttg gacggacggc 900gcagcagttg
acgcacacac gtacgccggc aaggtgtacg acttttacaa gacgaagttc 960ggcagaaaca
gccttgatgg aaatggactg ctgatcagaa gcagcgtcca ctacagcagc 1020agatacaata
acgccttctg gaacggcaca caaatcgtct ttggcgatgg agacggatca 1080acattcatcc
cgctgtcagg cgacctggac gttgtgggcc acgagctgag ccacggcgtc 1140atcgagtaca
cgagcaacct gcagtacctg aatgaaagcg gcgcactgaa cgagtcatat 1200gctgatgtgc
ttggcaatag catccaggcc aagaactggc ttatcggaga cgacgtctac 1260acacctggca
tcagcggcga tgctctgaga agcatgagca atcctacact ttacggccaa 1320ccggacaact
acgcgaatag atatacgggc agcagcgaca atggcggcgt tcatacaaac 1380tcaggcatca
cgaacaaggc gttctacctg ctggcacagg gaggcacgca aaacggcgtt 1440acagttgcgg
gcattggcag agatgcggcc gtcaacatct tctacaacac agtcgcctac 1500tacctgacga
gcacgtcaaa cttcgcagcg gcaaagaacg catcaattca agcagcaaag 1560gatctgtacg
gaacaggcag ctcatatgtc acgtcagtta cgaatgcgtt tagagccgtc 1620ggcctttaa
162915542PRTArtificial SequenceSynthetic amino acid sequence of the
PhuPro2 precursor protein expressed from plasmid pGX150(AprE-
PhuPro2) 15Met Arg Ser Lys Lys Leu Trp Ile Ser Leu Leu Phe Ala Leu Thr
Leu 1 5 10 15 Ile
Phe Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala Ala Gly Lys
20 25 30 Glu Ser Pro Ser Leu
Gly Ala Ala Gly Thr Pro Gly Val Ser Val Val 35
40 45 Asn Asn Gln Leu Val Thr Gln Phe Ile
Glu Ala Ser Lys Asp Ala Lys 50 55
60 Ile Val Pro Gly Ser Ser Glu Asp Lys Ile Trp Ala Phe
Leu Glu Gly 65 70 75
80 Gln Gln Ala Lys Leu Gly Val Ser Ala Ala Asp Val Lys Thr Ser Phe
85 90 95 Leu Ile Gln Lys
Lys Glu Val Asp Pro Thr Ser Gly Val Glu His Phe 100
105 110 Arg Leu Gln Gln Tyr Val Asn Gly Ile
Pro Val Tyr Gly Gly Asp Gln 115 120
125 Thr Ile His Ile Asp Lys Ala Gly Gln Val Thr Ser Phe Val
Gly Ala 130 135 140
Val Leu Pro Ala Gln Asn Gln Ile Thr Ala Lys Ser Ser Val Pro Ala 145
150 155 160 Ile Ser Ala Ser Asp
Ala Leu Ala Ile Ala Ala Lys Glu Ala Ser Ser 165
170 175 Arg Ile Gly Glu Leu Gly Ala Gln Glu Lys
Thr Pro Ser Ala Gln Leu 180 185
190 Tyr Val Tyr Pro Glu Gly Asn Gly Ser Arg Leu Val Tyr Gln Thr
Glu 195 200 205 Val
Asn Val Leu Glu Pro Gln Pro Leu Arg Thr Arg Tyr Leu Ile Asp 210
215 220 Ala Ala Asp Gly His Ile
Val Gln Gln Tyr Asp Leu Ile Glu Thr Ala 225 230
235 240 Thr Gly Ser Gly Thr Gly Val Leu Gly Asp Asn
Lys Thr Phe Gln Thr 245 250
255 Thr Leu Ser Gly Ser Thr Tyr Gln Leu Lys Asp Thr Thr Arg Gly Asn
260 265 270 Gly Ile
Tyr Thr Tyr Thr Ala Ser Asn Arg Thr Thr Ile Pro Gly Thr 275
280 285 Leu Leu Thr Asp Ala Asp Asn
Val Trp Thr Asp Gly Ala Ala Val Asp 290 295
300 Ala His Thr Tyr Ala Gly Lys Val Tyr Asp Phe Tyr
Lys Thr Lys Phe 305 310 315
320 Gly Arg Asn Ser Leu Asp Gly Asn Gly Leu Leu Ile Arg Ser Ser Val
325 330 335 His Tyr Ser
Ser Arg Tyr Asn Asn Ala Phe Trp Asn Gly Thr Gln Ile 340
345 350 Val Phe Gly Asp Gly Asp Gly Ser
Thr Phe Ile Pro Leu Ser Gly Asp 355 360
365 Leu Asp Val Val Gly His Glu Leu Ser His Gly Val Ile
Glu Tyr Thr 370 375 380
Ser Asn Leu Gln Tyr Leu Asn Glu Ser Gly Ala Leu Asn Glu Ser Tyr 385
390 395 400 Ala Asp Val Leu
Gly Asn Ser Ile Gln Ala Lys Asn Trp Leu Ile Gly 405
410 415 Asp Asp Val Tyr Thr Pro Gly Ile Ser
Gly Asp Ala Leu Arg Ser Met 420 425
430 Ser Asn Pro Thr Leu Tyr Gly Gln Pro Asp Asn Tyr Ala Asn
Arg Tyr 435 440 445
Thr Gly Ser Ser Asp Asn Gly Gly Val His Thr Asn Ser Gly Ile Thr 450
455 460 Asn Lys Ala Phe Tyr
Leu Leu Ala Gln Gly Gly Thr Gln Asn Gly Val 465 470
475 480 Thr Val Ala Gly Ile Gly Arg Asp Ala Ala
Val Asn Ile Phe Tyr Asn 485 490
495 Thr Val Ala Tyr Tyr Leu Thr Ser Thr Ser Asn Phe Ala Ala Ala
Lys 500 505 510 Asn
Ala Ser Ile Gln Ala Ala Lys Asp Leu Tyr Gly Thr Gly Ser Ser 515
520 525 Tyr Val Thr Ser Val Thr
Asn Ala Phe Arg Ala Val Gly Leu 530 535
540 161581DNAPaenibacillus
ehimensismisc_feature(1)..(1581)nucleotide sequence of the PehPro1 gene
isolated from Paenibacillus ehimensis 16atgttaaaag tatgggcatc
gattattaca ggagcatttt tgctcgggag cgtgcaaggg 60gtgcaagctg ctccacaaga
tcaagctgct cccttcggag gattcacccc tcaattgatt 120accggggaaa gctggagtgc
gccgcaagga gtatcgggag aggaaaaaat ctggaagtat 180ctcgaatcca agcaggaaag
cttccaaatc ggccaaaccg ttgatctgaa aaagcaattg 240aaaattatcg gccaaacgac
cgacgagaaa acgggaacca cgcattaccg tctacagcag 300tatgtgggag gcgtccccgt
atacggcggc gtacaaacga tccatgtcaa caaagaagga 360caagttacct cgctgatcgg
cagcctgctt cccgaccagc agcagcaagt ttcgaaaagc 420ttgaattcgc aaatcagcga
agcgcaagcc atcgccgtgg cccagaaaga taccgaggcc 480gccgtcggca agctgggtga
accgcaaaag acaccggaag cggatctgta cgtttattta 540cacaacggac aaccggtcct
cgcttatgtg accgaggtta acgttctcga accggaggca 600atccggacgc gctacttcat
cagcgccgaa gacggcagca ttttattcaa gtacgacatc 660ctcgctcacg ctacaggtac
cggaaaaggc gtgctcggag atacgaaatc gttcacgacc 720acgcaatccg gctccactta
tcaattgaag gatacgacgc gcgggcaagg tatcgtcact 780tacagcgctg gcaaccggtc
ctctctgccg ggaacgctgc tcaccagctc cagcaatatt 840tggaacgacg gcgcggcggt
cgatgcgcat gcctataccg ccaaagtgta cgattactat 900aaaaacaaat ttggccgcaa
cagcattgac ggcaacggct tccagcttaa atcgaccgtg 960cactattcct ccagatacaa
caacgccttc tggaacggtg tgcaaatggt gtacggcgac 1020ggcgacggcg taaccttcat
tccgttctcc gccgatccgg acgtcatcgg ccacgaattg 1080acccacggcg ttacggaaca
tacggccggc ctggaatact acggcgaatc cggagcgctg 1140aacgaatcga tctccgatat
tatcggcaac gcgatcgacg gcaaaaactg gctgatcggc 1200gacttgattt atacgccgaa
tactcccggg gacgccctcc gctctatgga gaaccccaag 1260ctgtataacc aacccgaccg
ctatcaagac cgctatacgg gaccttccga taacggcggc 1320gtgcatatta acagcggtat
caacaacaaa gccttctacc tgatcgccca aggcggcacg 1380cactatggcg tcaccgtgaa
cgggatcgga cgcgatgcgg ctgtgcaaat tttctatgac 1440gccctcatca attacctgac
tccaacttcg aacttctcgg cgatgcgcgc agcagccatt 1500caagcggcaa ccgacctgta
cggagcgaat tcttctcaag taaacgctgt caaaaaagcg 1560tatactgccg tcggcgtgaa c
158117527PRTPaenibacillus
ehimensismisc_feature(1)..(527)amino acid sequence of the PehPro1
precursor protein 17Met Leu Lys Val Trp Ala Ser Ile Ile Thr Gly Ala
Phe Leu Leu Gly 1 5 10
15 Ser Val Gln Gly Val Gln Ala Ala Pro Gln Asp Gln Ala Ala Pro Phe
20 25 30 Gly Gly Phe
Thr Pro Gln Leu Ile Thr Gly Glu Ser Trp Ser Ala Pro 35
40 45 Gln Gly Val Ser Gly Glu Glu Lys
Ile Trp Lys Tyr Leu Glu Ser Lys 50 55
60 Gln Glu Ser Phe Gln Ile Gly Gln Thr Val Asp Leu Lys
Lys Gln Leu 65 70 75
80 Lys Ile Ile Gly Gln Thr Thr Asp Glu Lys Thr Gly Thr Thr His Tyr
85 90 95 Arg Leu Gln Gln
Tyr Val Gly Gly Val Pro Val Tyr Gly Gly Val Gln 100
105 110 Thr Ile His Val Asn Lys Glu Gly Gln
Val Thr Ser Leu Ile Gly Ser 115 120
125 Leu Leu Pro Asp Gln Gln Gln Gln Val Ser Lys Ser Leu Asn
Ser Gln 130 135 140
Ile Ser Glu Ala Gln Ala Ile Ala Val Ala Gln Lys Asp Thr Glu Ala 145
150 155 160 Ala Val Gly Lys Leu
Gly Glu Pro Gln Lys Thr Pro Glu Ala Asp Leu 165
170 175 Tyr Val Tyr Leu His Asn Gly Gln Pro Val
Leu Ala Tyr Val Thr Glu 180 185
190 Val Asn Val Leu Glu Pro Glu Ala Ile Arg Thr Arg Tyr Phe Ile
Ser 195 200 205 Ala
Glu Asp Gly Ser Ile Leu Phe Lys Tyr Asp Ile Leu Ala His Ala 210
215 220 Thr Gly Thr Gly Lys Gly
Val Leu Gly Asp Thr Lys Ser Phe Thr Thr 225 230
235 240 Thr Gln Ser Gly Ser Thr Tyr Gln Leu Lys Asp
Thr Thr Arg Gly Gln 245 250
255 Gly Ile Val Thr Tyr Ser Ala Gly Asn Arg Ser Ser Leu Pro Gly Thr
260 265 270 Leu Leu
Thr Ser Ser Ser Asn Ile Trp Asn Asp Gly Ala Ala Val Asp 275
280 285 Ala His Ala Tyr Thr Ala Lys
Val Tyr Asp Tyr Tyr Lys Asn Lys Phe 290 295
300 Gly Arg Asn Ser Ile Asp Gly Asn Gly Phe Gln Leu
Lys Ser Thr Val 305 310 315
320 His Tyr Ser Ser Arg Tyr Asn Asn Ala Phe Trp Asn Gly Val Gln Met
325 330 335 Val Tyr Gly
Asp Gly Asp Gly Val Thr Phe Ile Pro Phe Ser Ala Asp 340
345 350 Pro Asp Val Ile Gly His Glu Leu
Thr His Gly Val Thr Glu His Thr 355 360
365 Ala Gly Leu Glu Tyr Tyr Gly Glu Ser Gly Ala Leu Asn
Glu Ser Ile 370 375 380
Ser Asp Ile Ile Gly Asn Ala Ile Asp Gly Lys Asn Trp Leu Ile Gly 385
390 395 400 Asp Leu Ile Tyr
Thr Pro Asn Thr Pro Gly Asp Ala Leu Arg Ser Met 405
410 415 Glu Asn Pro Lys Leu Tyr Asn Gln Pro
Asp Arg Tyr Gln Asp Arg Tyr 420 425
430 Thr Gly Pro Ser Asp Asn Gly Gly Val His Ile Asn Ser Gly
Ile Asn 435 440 445
Asn Lys Ala Phe Tyr Leu Ile Ala Gln Gly Gly Thr His Tyr Gly Val 450
455 460 Thr Val Asn Gly Ile
Gly Arg Asp Ala Ala Val Gln Ile Phe Tyr Asp 465 470
475 480 Ala Leu Ile Asn Tyr Leu Thr Pro Thr Ser
Asn Phe Ser Ala Met Arg 485 490
495 Ala Ala Ala Ile Gln Ala Ala Thr Asp Leu Tyr Gly Ala Asn Ser
Ser 500 505 510 Gln
Val Asn Ala Val Lys Lys Ala Tyr Thr Ala Val Gly Val Asn 515
520 525 18304PRTPaenibacillus
ehimensismisc_feature(1)..(304)amino acid sequence of the predicted
mature form of PehPro1 18Ala Thr Gly Thr Gly Lys Gly Val Leu Gly Asp
Thr Lys Ser Phe Thr 1 5 10
15 Thr Thr Gln Ser Gly Ser Thr Tyr Gln Leu Lys Asp Thr Thr Arg Gly
20 25 30 Gln Gly
Ile Val Thr Tyr Ser Ala Gly Asn Arg Ser Ser Leu Pro Gly 35
40 45 Thr Leu Leu Thr Ser Ser Ser
Asn Ile Trp Asn Asp Gly Ala Ala Val 50 55
60 Asp Ala His Ala Tyr Thr Ala Lys Val Tyr Asp Tyr
Tyr Lys Asn Lys 65 70 75
80 Phe Gly Arg Asn Ser Ile Asp Gly Asn Gly Phe Gln Leu Lys Ser Thr
85 90 95 Val His Tyr
Ser Ser Arg Tyr Asn Asn Ala Phe Trp Asn Gly Val Gln 100
105 110 Met Val Tyr Gly Asp Gly Asp Gly
Val Thr Phe Ile Pro Phe Ser Ala 115 120
125 Asp Pro Asp Val Ile Gly His Glu Leu Thr His Gly Val
Thr Glu His 130 135 140
Thr Ala Gly Leu Glu Tyr Tyr Gly Glu Ser Gly Ala Leu Asn Glu Ser 145
150 155 160 Ile Ser Asp Ile
Ile Gly Asn Ala Ile Asp Gly Lys Asn Trp Leu Ile 165
170 175 Gly Asp Leu Ile Tyr Thr Pro Asn Thr
Pro Gly Asp Ala Leu Arg Ser 180 185
190 Met Glu Asn Pro Lys Leu Tyr Asn Gln Pro Asp Arg Tyr Gln
Asp Arg 195 200 205
Tyr Thr Gly Pro Ser Asp Asn Gly Gly Val His Ile Asn Ser Gly Ile 210
215 220 Asn Asn Lys Ala Phe
Tyr Leu Ile Ala Gln Gly Gly Thr His Tyr Gly 225 230
235 240 Val Thr Val Asn Gly Ile Gly Arg Asp Ala
Ala Val Gln Ile Phe Tyr 245 250
255 Asp Ala Leu Ile Asn Tyr Leu Thr Pro Thr Ser Asn Phe Ser Ala
Met 260 265 270 Arg
Ala Ala Ala Ile Gln Ala Ala Thr Asp Leu Tyr Gly Ala Asn Ser 275
280 285 Ser Gln Val Asn Ala Val
Lys Lys Ala Tyr Thr Ala Val Gly Val Asn 290 295
300 191611DNAArtificial SequenceSynthetic
nucleotide sequence of the synthesized PehPro1 gene in plasmid
pGX148(AprE- PehPro1) 19gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt
taacgttaat ctttacgatg 60gcgttcagca acatgagcgc gcaggctgct ggaaaagcac
ctcaagatca ggcagcacct 120tttggaggct ttacaccgca acttatcaca ggcgaatcat
ggtcagcacc gcagggcgtt 180tcaggcgagg aaaagatctg gaagtacctt gagagcaagc
aggagtcatt tcaaatcggc 240cagacagtcg acctgaaaaa gcaactgaag atcatcggcc
aaacaacgga cgaaaagacg 300ggcacgacgc attatagact gcaacaatat gttggcggcg
tgccggttta tggaggcgtg 360caaacaatcc acgtgaacaa ggaaggacag gtcacgtcac
tgatcggcag cctgctgccg 420gatcagcagc aacaagtctc aaagagcctg aactcacaaa
ttagcgaggc acaagcgatt 480gcagttgcac aaaaggacac ggaagcagct gtcggcaagc
tgggcgaacc gcaaaaaaca 540cctgaggctg acctttacgt ctacctgcat aacggccagc
cggtccttgc gtacgttacg 600gaagttaacg tgctggagcc ggaggccatc agaacgagat
acttcattag cgcggaggat 660ggaagcattc tgtttaagta cgatattctt gctcacgcga
caggcacagg caagggcgtc 720cttggcgaca caaaaagctt cacgacaacg cagagcggat
caacgtacca gctgaaagat 780acaacaagag gacaaggcat cgttacgtat tcagcgggca
atagatcaag cctgccgggc 840acactgctga catcaagctc aaacatttgg aatgacggcg
cagcagttga tgcccatgcg 900tacacagcca aggtgtacga ctactataag aacaagtttg
gcagaaatag catcgacgga 960aatggatttc aacttaaatc aacggtgcac tactcatcaa
gatataacaa tgcgttttgg 1020aacggagtgc agatggtcta cggagacggc gacggcgtga
catttattcc gtttagcgcc 1080gacccggacg tgattggaca tgaactgaca catggagtga
cagagcatac ggcgggactg 1140gaatattacg gcgaaagcgg cgcactgaac gaaagcatct
cagacattat tggaaacgca 1200atcgatggca aaaactggct gattggcgat ctgatttata
cgccgaatac accgggcgat 1260gcactgagat caatggagaa tccgaagctg tacaaccaac
cggacagata ccaagataga 1320tacacaggac cgtcagacaa cggcggagtc catatcaaca
gcggaatcaa taacaaagcc 1380ttttacctga tcgcccaagg cggaacgcac tatggcgtta
cagtcaatgg catcggaaga 1440gatgccgcag ttcagatttt ctatgacgcg ctgatcaact
atctgacgcc tacaagcaat 1500ttctcagcaa tgagagccgc agcaatccaa gcagccacgg
atctgtatgg agccaattca 1560tcacaagtta atgctgttaa gaaggcttat acggcagtgg
gagttaacta a 161120536PRTArtificial SequenceSynthetic amino
acid sequence of the PehPro1 precursor protein expressed from
plasmid pGX148(AprE- PehPro1) 20Met Arg Ser Lys Lys Leu Trp Ile Ser Leu
Leu Phe Ala Leu Thr Leu 1 5 10
15 Ile Phe Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala Ala Gly
Lys 20 25 30 Ala
Pro Gln Asp Gln Ala Ala Pro Phe Gly Gly Phe Thr Pro Gln Leu 35
40 45 Ile Thr Gly Glu Ser Trp
Ser Ala Pro Gln Gly Val Ser Gly Glu Glu 50 55
60 Lys Ile Trp Lys Tyr Leu Glu Ser Lys Gln Glu
Ser Phe Gln Ile Gly 65 70 75
80 Gln Thr Val Asp Leu Lys Lys Gln Leu Lys Ile Ile Gly Gln Thr Thr
85 90 95 Asp Glu
Lys Thr Gly Thr Thr His Tyr Arg Leu Gln Gln Tyr Val Gly 100
105 110 Gly Val Pro Val Tyr Gly Gly
Val Gln Thr Ile His Val Asn Lys Glu 115 120
125 Gly Gln Val Thr Ser Leu Ile Gly Ser Leu Leu Pro
Asp Gln Gln Gln 130 135 140
Gln Val Ser Lys Ser Leu Asn Ser Gln Ile Ser Glu Ala Gln Ala Ile 145
150 155 160 Ala Val Ala
Gln Lys Asp Thr Glu Ala Ala Val Gly Lys Leu Gly Glu 165
170 175 Pro Gln Lys Thr Pro Glu Ala Asp
Leu Tyr Val Tyr Leu His Asn Gly 180 185
190 Gln Pro Val Leu Ala Tyr Val Thr Glu Val Asn Val Leu
Glu Pro Glu 195 200 205
Ala Ile Arg Thr Arg Tyr Phe Ile Ser Ala Glu Asp Gly Ser Ile Leu 210
215 220 Phe Lys Tyr Asp
Ile Leu Ala His Ala Thr Gly Thr Gly Lys Gly Val 225 230
235 240 Leu Gly Asp Thr Lys Ser Phe Thr Thr
Thr Gln Ser Gly Ser Thr Tyr 245 250
255 Gln Leu Lys Asp Thr Thr Arg Gly Gln Gly Ile Val Thr Tyr
Ser Ala 260 265 270
Gly Asn Arg Ser Ser Leu Pro Gly Thr Leu Leu Thr Ser Ser Ser Asn
275 280 285 Ile Trp Asn Asp
Gly Ala Ala Val Asp Ala His Ala Tyr Thr Ala Lys 290
295 300 Val Tyr Asp Tyr Tyr Lys Asn Lys
Phe Gly Arg Asn Ser Ile Asp Gly 305 310
315 320 Asn Gly Phe Gln Leu Lys Ser Thr Val His Tyr Ser
Ser Arg Tyr Asn 325 330
335 Asn Ala Phe Trp Asn Gly Val Gln Met Val Tyr Gly Asp Gly Asp Gly
340 345 350 Val Thr Phe
Ile Pro Phe Ser Ala Asp Pro Asp Val Ile Gly His Glu 355
360 365 Leu Thr His Gly Val Thr Glu His
Thr Ala Gly Leu Glu Tyr Tyr Gly 370 375
380 Glu Ser Gly Ala Leu Asn Glu Ser Ile Ser Asp Ile Ile
Gly Asn Ala 385 390 395
400 Ile Asp Gly Lys Asn Trp Leu Ile Gly Asp Leu Ile Tyr Thr Pro Asn
405 410 415 Thr Pro Gly Asp
Ala Leu Arg Ser Met Glu Asn Pro Lys Leu Tyr Asn 420
425 430 Gln Pro Asp Arg Tyr Gln Asp Arg Tyr
Thr Gly Pro Ser Asp Asn Gly 435 440
445 Gly Val His Ile Asn Ser Gly Ile Asn Asn Lys Ala Phe Tyr
Leu Ile 450 455 460
Ala Gln Gly Gly Thr His Tyr Gly Val Thr Val Asn Gly Ile Gly Arg 465
470 475 480 Asp Ala Ala Val Gln
Ile Phe Tyr Asp Ala Leu Ile Asn Tyr Leu Thr 485
490 495 Pro Thr Ser Asn Phe Ser Ala Met Arg Ala
Ala Ala Ile Gln Ala Ala 500 505
510 Thr Asp Leu Tyr Gly Ala Asn Ser Ser Gln Val Asn Ala Val Lys
Lys 515 520 525 Ala
Tyr Thr Ala Val Gly Val Asn 530 535
211563DNAPaenibacillus barcinonensismisc_feature(1)..(1563)nucleotide
sequence of the PbaPro1 gene isolated from Paenibacillus
barcinonensis 21atgaaattga ccaaaattat gccaacaatt cttgcaggag ctcttttgct
cacatccctg 60tcctctgcag cagcaatgcc gttatctgac tcatccattc catttgaggg
cccctacacc 120tccgaggaga gtattctgtt gaacaacaac ccggacgaaa tgatttataa
ttttcttgca 180caacaagagc aatttctgaa tgccgacgtc aaaggacagc tcaaaatcat
taaacgcaac 240acagacactt ccggcatcag acactttcgt ctgaagcaat acatcaaagg
tgttccggtt 300tacggcgcag aacaaacgat ccatctggac aagaacggag ctgtaacttc
cgcactcggc 360gatcttccgc caattgaaga acaggctgtt ccgaatgatg gcgttcccgc
aatcagtgca 420gacgatgcca tccgtgccgc cgagaatgaa gccacctccc gtcttggaga
gcttggcgca 480ccagagcttg agccaaaggc cgaattaaac atttatcatc atgaagatga
cggacaaacc 540tacctcgttt acattacgga agttaacgtg cttgagcctt ccccgctacg
gaccaaatat 600tttattaacg cccttgatgg aagcatcgta tctcaatacg atattatcaa
ctttgccaca 660ggcaccggta caggcgtgca tggtgatacc aaaacactga cgacaactca
atccggcagc 720acctatcagc tgaaagatac aactcgtgga aaaggcattc aaacctatac
tgcgaacaat 780cgctcctcgc ttccaggcag cttgtctacc agttccaata acgtatggac
agaccgtgca 840gctgtagatg cgcacgccta tgctgccgcc acatatgact tctacaaaaa
caaattcaat 900cgcaacggca ttgacggaaa cgggctgttg attcgctcta cagtgcatta
tggctccaac 960tataaaaacg ccttctggaa cggagcacag attgtctatg gagatggcga
tggcatcgag 1020ttcggtccct tctccggtga tctcgatgtt gtcggacatg aattgacaca
cggggtgatt 1080gaatatacag ccaatctcga atatcgcaat gagccgggtg ctttaaacga
agcttttgcc 1140gacattatgg ggaacaccat cgaaagcaaa aactggctgc ttggcgacgg
aatctatact 1200ccaaacattc caggtgatgc cctgcgctcg ttatccgacc ctacgctgta
taaccagcct 1260gacaaataca gtgatcgcta cactggctct caggataatg gcggtgtgca
tatcaacagc 1320gggatcatta acaaagcata ttatcttgca gcccaaggcg gtactcataa
cggggtaacc 1380gttagcggca tcggccggga taaagcagta cgtattttct atagcacgct
ggtgaactac 1440ctgacgccaa cctccaaatt tgcagcagcc aaaacagcga caattcaggc
agccaaggac 1500ctgtacggtg ccaattccgc tgaagctacg gcaatcacca aagcttatca
agcggtaggt 1560ttg
156322521PRTPaenibacillus
barcinonensismisc_feature(1)..(521)amino acid sequence of the PbaPro1
precursor protein 22Met Lys Leu Thr Lys Ile Met Pro Thr Ile Leu Ala
Gly Ala Leu Leu 1 5 10
15 Leu Thr Ser Leu Ser Ser Ala Ala Ala Met Pro Leu Ser Asp Ser Ser
20 25 30 Ile Pro Phe
Glu Gly Pro Tyr Thr Ser Glu Glu Ser Ile Leu Leu Asn 35
40 45 Asn Asn Pro Asp Glu Met Ile Tyr
Asn Phe Leu Ala Gln Gln Glu Gln 50 55
60 Phe Leu Asn Ala Asp Val Lys Gly Gln Leu Lys Ile Ile
Lys Arg Asn 65 70 75
80 Thr Asp Thr Ser Gly Ile Arg His Phe Arg Leu Lys Gln Tyr Ile Lys
85 90 95 Gly Val Pro Val
Tyr Gly Ala Glu Gln Thr Ile His Leu Asp Lys Asn 100
105 110 Gly Ala Val Thr Ser Ala Leu Gly Asp
Leu Pro Pro Ile Glu Glu Gln 115 120
125 Ala Val Pro Asn Asp Gly Val Pro Ala Ile Ser Ala Asp Asp
Ala Ile 130 135 140
Arg Ala Ala Glu Asn Glu Ala Thr Ser Arg Leu Gly Glu Leu Gly Ala 145
150 155 160 Pro Glu Leu Glu Pro
Lys Ala Glu Leu Asn Ile Tyr His His Glu Asp 165
170 175 Asp Gly Gln Thr Tyr Leu Val Tyr Ile Thr
Glu Val Asn Val Leu Glu 180 185
190 Pro Ser Pro Leu Arg Thr Lys Tyr Phe Ile Asn Ala Leu Asp Gly
Ser 195 200 205 Ile
Val Ser Gln Tyr Asp Ile Ile Asn Phe Ala Thr Gly Thr Gly Thr 210
215 220 Gly Val His Gly Asp Thr
Lys Thr Leu Thr Thr Thr Gln Ser Gly Ser 225 230
235 240 Thr Tyr Gln Leu Lys Asp Thr Thr Arg Gly Lys
Gly Ile Gln Thr Tyr 245 250
255 Thr Ala Asn Asn Arg Ser Ser Leu Pro Gly Ser Leu Ser Thr Ser Ser
260 265 270 Asn Asn
Val Trp Thr Asp Arg Ala Ala Val Asp Ala His Ala Tyr Ala 275
280 285 Ala Ala Thr Tyr Asp Phe Tyr
Lys Asn Lys Phe Asn Arg Asn Gly Ile 290 295
300 Asp Gly Asn Gly Leu Leu Ile Arg Ser Thr Val His
Tyr Gly Ser Asn 305 310 315
320 Tyr Lys Asn Ala Phe Trp Asn Gly Ala Gln Ile Val Tyr Gly Asp Gly
325 330 335 Asp Gly Ile
Glu Phe Gly Pro Phe Ser Gly Asp Leu Asp Val Val Gly 340
345 350 His Glu Leu Thr His Gly Val Ile
Glu Tyr Thr Ala Asn Leu Glu Tyr 355 360
365 Arg Asn Glu Pro Gly Ala Leu Asn Glu Ala Phe Ala Asp
Ile Met Gly 370 375 380
Asn Thr Ile Glu Ser Lys Asn Trp Leu Leu Gly Asp Gly Ile Tyr Thr 385
390 395 400 Pro Asn Ile Pro
Gly Asp Ala Leu Arg Ser Leu Ser Asp Pro Thr Leu 405
410 415 Tyr Asn Gln Pro Asp Lys Tyr Ser Asp
Arg Tyr Thr Gly Ser Gln Asp 420 425
430 Asn Gly Gly Val His Ile Asn Ser Gly Ile Ile Asn Lys Ala
Tyr Tyr 435 440 445
Leu Ala Ala Gln Gly Gly Thr His Asn Gly Val Thr Val Ser Gly Ile 450
455 460 Gly Arg Asp Lys Ala
Val Arg Ile Phe Tyr Ser Thr Leu Val Asn Tyr 465 470
475 480 Leu Thr Pro Thr Ser Lys Phe Ala Ala Ala
Lys Thr Ala Thr Ile Gln 485 490
495 Ala Ala Lys Asp Leu Tyr Gly Ala Asn Ser Ala Glu Ala Thr Ala
Ile 500 505 510 Thr
Lys Ala Tyr Gln Ala Val Gly Leu 515 520
23303PRTPaenibacillus barcinonensismisc_feature(1)..(303)amino acid
sequence of the predicted mature form of PbaPro1 23Ala Thr Gly Thr
Gly Thr Gly Val His Gly Asp Thr Lys Thr Leu Thr 1 5
10 15 Thr Thr Gln Ser Gly Ser Thr Tyr Gln
Leu Lys Asp Thr Thr Arg Gly 20 25
30 Lys Gly Ile Gln Thr Tyr Thr Ala Asn Asn Arg Ser Ser Leu
Pro Gly 35 40 45
Ser Leu Ser Thr Ser Ser Asn Asn Val Trp Thr Asp Arg Ala Ala Val 50
55 60 Asp Ala His Ala Tyr
Ala Ala Ala Thr Tyr Asp Phe Tyr Lys Asn Lys 65 70
75 80 Phe Asn Arg Asn Gly Ile Asp Gly Asn Gly
Leu Leu Ile Arg Ser Thr 85 90
95 Val His Tyr Gly Ser Asn Tyr Lys Asn Ala Phe Trp Asn Gly Ala
Gln 100 105 110 Ile
Val Tyr Gly Asp Gly Asp Gly Ile Glu Phe Gly Pro Phe Ser Gly 115
120 125 Asp Leu Asp Val Val Gly
His Glu Leu Thr His Gly Val Ile Glu Tyr 130 135
140 Thr Ala Asn Leu Glu Tyr Arg Asn Glu Pro Gly
Ala Leu Asn Glu Ala 145 150 155
160 Phe Ala Asp Ile Met Gly Asn Thr Ile Glu Ser Lys Asn Trp Leu Leu
165 170 175 Gly Asp
Gly Ile Tyr Thr Pro Asn Ile Pro Gly Asp Ala Leu Arg Ser 180
185 190 Leu Ser Asp Pro Thr Leu Tyr
Asn Gln Pro Asp Lys Tyr Ser Asp Arg 195 200
205 Tyr Thr Gly Ser Gln Asp Asn Gly Gly Val His Ile
Asn Ser Gly Ile 210 215 220
Ile Asn Lys Ala Tyr Tyr Leu Ala Ala Gln Gly Gly Thr His Asn Gly 225
230 235 240 Val Thr Val
Ser Gly Ile Gly Arg Asp Lys Ala Val Arg Ile Phe Tyr 245
250 255 Ser Thr Leu Val Asn Tyr Leu Thr
Pro Thr Ser Lys Phe Ala Ala Ala 260 265
270 Lys Thr Ala Thr Ile Gln Ala Ala Lys Asp Leu Tyr Gly
Ala Asn Ser 275 280 285
Ala Glu Ala Thr Ala Ile Thr Lys Ala Tyr Gln Ala Val Gly Leu 290
295 300 241587DNAArtificial
SequenceSynthetic nucleotide sequence of the synthesized PbaPro1
gene in plasmid pGX147(AprE- PbaPro1) 24gtgagaagca aaaaattgtg gatcagcttg
ttgtttgcgt taacgttaat ctttacgatg 60gcgttcagca acatgagcgc gcaggctgct
ggaaaaatgc ctctgtcaga cagcagcatt 120ccgtttgagg gcccgtacac atcagaagaa
agcatcctgc tgaacaacaa cccggacgag 180atgatctaca atttcctggc acagcaggag
cagttcctga acgcagacgt gaagggccag 240ctgaaaatca tcaaaagaaa cacagacacg
agcggcatca gacacttcag actgaagcag 300tacatcaagg gcgtcccggt ttacggcgct
gagcagacaa tccacctgga caaaaatggc 360gcagtgacga gcgcacttgg agatctgccg
ccgattgaag agcaagcagt cccgaacgat 420ggcgttccgg cgattagcgc tgatgacgct
atcagagccg cggaaaacga agcgacgtca 480agactgggag aacttggcgc accggaactt
gaaccgaagg cggaactgaa catctatcac 540cacgaagacg atggacagac gtacctggtg
tacatcacgg aggtgaatgt gctggagccg 600tcaccgctga gaacaaaata cttcatcaat
gcgctggatg gcagcatcgt tagccaatac 660gacatcatta acttcgccac aggcacgggc
acaggcgttc atggcgacac aaaaacgctt 720acgacaacac agtcaggctc aacgtaccag
ctgaaagaca caacaagagg caagggcatc 780cagacgtata cagccaataa cagaagctca
cttccgggct cactgtcaac aagcagcaat 840aatgtctgga cggacagagc tgcagtggac
gcgcacgcgt atgctgcggc cacgtacgac 900ttctacaaga acaagttcaa cagaaacggc
attgatggca acggcctgct tattagaagc 960acggtccact acggctcaaa ctacaagaat
gcgttttgga acggcgccca aattgtttat 1020ggcgatggag acggcatcga gttcggacct
tttagcggcg acctggatgt ggtcggacat 1080gaactgacgc acggcgttat cgagtatacg
gcgaatctgg aatacagaaa tgaaccgggc 1140gctctgaatg aggccttcgc ggatatcatg
ggcaacacaa ttgagagcaa aaactggctt 1200ctgggcgacg gaatctacac gccgaacatt
ccgggagatg cactgagatc actgagcgac 1260cctacgctgt acaaccagcc ggacaaatac
agcgacagat acacgggatc acaggacaat 1320ggcggcgtcc atattaactc aggcatcatc
aacaaagcgt attatctggc agctcaaggc 1380ggcacgcata atggcgtcac agttagcgga
atcggcagag acaaggccgt cagaattttc 1440tactcaacgc tggtgaacta cctgacaccg
acaagcaagt ttgcagccgc caaaacagcc 1500acgattcagg cagcaaagga cctgtacgga
gcgaactcag cagaggccac agcgattacg 1560aaggcttatc aagccgtggg actgtaa
158725528PRTArtificial SequenceSynthetic
amino acid sequence of the PbaPro1 precursor protein expressed from
plasmid pGX147(AprE-PbaPro1) 25Met Arg Ser Lys Lys Leu Trp Ile Ser Leu
Leu Phe Ala Leu Thr Leu 1 5 10
15 Ile Phe Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala Ala Gly
Lys 20 25 30 Met
Pro Leu Ser Asp Ser Ser Ile Pro Phe Glu Gly Pro Tyr Thr Ser 35
40 45 Glu Glu Ser Ile Leu Leu
Asn Asn Asn Pro Asp Glu Met Ile Tyr Asn 50 55
60 Phe Leu Ala Gln Gln Glu Gln Phe Leu Asn Ala
Asp Val Lys Gly Gln 65 70 75
80 Leu Lys Ile Ile Lys Arg Asn Thr Asp Thr Ser Gly Ile Arg His Phe
85 90 95 Arg Leu
Lys Gln Tyr Ile Lys Gly Val Pro Val Tyr Gly Ala Glu Gln 100
105 110 Thr Ile His Leu Asp Lys Asn
Gly Ala Val Thr Ser Ala Leu Gly Asp 115 120
125 Leu Pro Pro Ile Glu Glu Gln Ala Val Pro Asn Asp
Gly Val Pro Ala 130 135 140
Ile Ser Ala Asp Asp Ala Ile Arg Ala Ala Glu Asn Glu Ala Thr Ser 145
150 155 160 Arg Leu Gly
Glu Leu Gly Ala Pro Glu Leu Glu Pro Lys Ala Glu Leu 165
170 175 Asn Ile Tyr His His Glu Asp Asp
Gly Gln Thr Tyr Leu Val Tyr Ile 180 185
190 Thr Glu Val Asn Val Leu Glu Pro Ser Pro Leu Arg Thr
Lys Tyr Phe 195 200 205
Ile Asn Ala Leu Asp Gly Ser Ile Val Ser Gln Tyr Asp Ile Ile Asn 210
215 220 Phe Ala Thr Gly
Thr Gly Thr Gly Val His Gly Asp Thr Lys Thr Leu 225 230
235 240 Thr Thr Thr Gln Ser Gly Ser Thr Tyr
Gln Leu Lys Asp Thr Thr Arg 245 250
255 Gly Lys Gly Ile Gln Thr Tyr Thr Ala Asn Asn Arg Ser Ser
Leu Pro 260 265 270
Gly Ser Leu Ser Thr Ser Ser Asn Asn Val Trp Thr Asp Arg Ala Ala
275 280 285 Val Asp Ala His
Ala Tyr Ala Ala Ala Thr Tyr Asp Phe Tyr Lys Asn 290
295 300 Lys Phe Asn Arg Asn Gly Ile Asp
Gly Asn Gly Leu Leu Ile Arg Ser 305 310
315 320 Thr Val His Tyr Gly Ser Asn Tyr Lys Asn Ala Phe
Trp Asn Gly Ala 325 330
335 Gln Ile Val Tyr Gly Asp Gly Asp Gly Ile Glu Phe Gly Pro Phe Ser
340 345 350 Gly Asp Leu
Asp Val Val Gly His Glu Leu Thr His Gly Val Ile Glu 355
360 365 Tyr Thr Ala Asn Leu Glu Tyr Arg
Asn Glu Pro Gly Ala Leu Asn Glu 370 375
380 Ala Phe Ala Asp Ile Met Gly Asn Thr Ile Glu Ser Lys
Asn Trp Leu 385 390 395
400 Leu Gly Asp Gly Ile Tyr Thr Pro Asn Ile Pro Gly Asp Ala Leu Arg
405 410 415 Ser Leu Ser Asp
Pro Thr Leu Tyr Asn Gln Pro Asp Lys Tyr Ser Asp 420
425 430 Arg Tyr Thr Gly Ser Gln Asp Asn Gly
Gly Val His Ile Asn Ser Gly 435 440
445 Ile Ile Asn Lys Ala Tyr Tyr Leu Ala Ala Gln Gly Gly Thr
His Asn 450 455 460
Gly Val Thr Val Ser Gly Ile Gly Arg Asp Lys Ala Val Arg Ile Phe 465
470 475 480 Tyr Ser Thr Leu Val
Asn Tyr Leu Thr Pro Thr Ser Lys Phe Ala Ala 485
490 495 Ala Lys Thr Ala Thr Ile Gln Ala Ala Lys
Asp Leu Tyr Gly Ala Asn 500 505
510 Ser Ala Glu Ala Thr Ala Ile Thr Lys Ala Tyr Gln Ala Val Gly
Leu 515 520 525
261779DNAPaenibacillus polymyxa SC2misc_feature(1)..(1779)nucleotide
sequence of the PpoPro1 gene identified from NCBI database
26atgaaaaaag tatgggtttc gcttcttgga ggagctatgt tattagggtc tgtcgcgtct
60ggtgcatctg cggagagttc cgtttcgggg ccagctcagc ttacaccgac cttccacgcc
120gagcaatgga aagcacctac ctcggtatcg ggggatgaca ttgtatggag ctatttaaat
180cgacaaaaga aatcgttgct gggtgtggat agctccagtg tacgtgaaca attccgaatc
240gttgatcgca caagcgacaa atccggtgta agccattatc gactgaagca gtatgtaaac
300ggaattcccg tgtatggagc tgaacaaact attcatgtgg gcaaatctgg tgaggtcacc
360tcttacttag gagcggtggt taatgaggat cagcaggcag aagctacgca aggtacaact
420ccaaaaatca gcgcttctga agcggtctac accgcatata aagaagcagc tgcacggatt
480gaagccctcc ctacctccga cgatactatt tctaaagacg ctgaggagcc aagcagtgta
540agtaaagata cttacgccga agcagctaac aacgaaaaaa cgctttctgt tgataaggac
600gagctgagtc ttgatcaggc atctgtcctg aaagatagca aaattgaagc agtggaacca
660gaaaaaagtt ccattgccaa aatcgctaat ctgcagcctg aagtagatcc taaagcagaa
720ctctactact accctaaggg ggatgacctg ctgctggttt atgtaacaga agttaatgtt
780ttagaacctg ccccactgcg tacccgctac attattgatg ccaatgacgg cagcatcgta
840ttccagtatg acatcattaa tgaagcgaca ggcacaggta aaggtgtgct tggtgattcc
900aaatcgttca ctactaccgc ttccggcagt agctaccagt taaaagatac aacacgcggt
960aacggaatcg tgacttacac ggcctccaac cgtcaaagca tcccaggtac cattttgaca
1020gatgccgata atgtatggaa tgatccagct ggtgtggacg cccatgcgta tgctgctaaa
1080acctatgatt actataaagc caaatttgga cgcaacagca ttgacggacg cggtctgcaa
1140cttcgttcga cggtccatta cggtagtcgc tacaacaatg ccttctggaa cggctcccaa
1200atgacttatg gagatggaga tggtagcaca tttatcgcct tcagcgggga ccccgatgta
1260gtaggacatg aacttacgca tggtgtcaca gagtatactt cgaatttgga atattacgga
1320gagtccggcg cattgaatga agctttctca gacgttatcg ggaatgacat tcagcgcaaa
1380aactggcttg taggcgatga tatttacacg ccaaacattg caggcgatgc ccttcgctca
1440atgtccaatc caaccctgta cgatcaacca gatcactatt ccaacctgta cagaggcagc
1500tccgataacg gcggtgttca caccaacagc ggtattatca ataaagctta ctacttgtta
1560gcacaaggtg gtaatttcca tggcgtaact gtaaatggaa ttggccgtga tgcagcggtg
1620caaatttact acagtgcctt tacgaactac ctgacttctt cttccgactt ctccaacgca
1680cgtgctgctg tgatccaagc cgcaaaagat ctgtacgggg cgaactcagc agaagcaact
1740gcagctgcca agtcttttga cgctgtaggc gtaaactaa
177927592PRTPaenibacillus polymyxa SC2misc_feature(1)..(592)amino acid
sequence of the PpoPro1 precursor protein 27Met Lys Lys Val Trp Val
Ser Leu Leu Gly Gly Ala Met Leu Leu Gly 1 5
10 15 Ser Val Ala Ser Gly Ala Ser Ala Glu Ser Ser
Val Ser Gly Pro Ala 20 25
30 Gln Leu Thr Pro Thr Phe His Ala Glu Gln Trp Lys Ala Pro Thr
Ser 35 40 45 Val
Ser Gly Asp Asp Ile Val Trp Ser Tyr Leu Asn Arg Gln Lys Lys 50
55 60 Ser Leu Leu Gly Val Asp
Ser Ser Ser Val Arg Glu Gln Phe Arg Ile 65 70
75 80 Val Asp Arg Thr Ser Asp Lys Ser Gly Val Ser
His Tyr Arg Leu Lys 85 90
95 Gln Tyr Val Asn Gly Ile Pro Val Tyr Gly Ala Glu Gln Thr Ile His
100 105 110 Val Gly
Lys Ser Gly Glu Val Thr Ser Tyr Leu Gly Ala Val Val Asn 115
120 125 Glu Asp Gln Gln Ala Glu Ala
Thr Gln Gly Thr Thr Pro Lys Ile Ser 130 135
140 Ala Ser Glu Ala Val Tyr Thr Ala Tyr Lys Glu Ala
Ala Ala Arg Ile 145 150 155
160 Glu Ala Leu Pro Thr Ser Asp Asp Thr Ile Ser Lys Asp Ala Glu Glu
165 170 175 Pro Ser Ser
Val Ser Lys Asp Thr Tyr Ala Glu Ala Ala Asn Asn Glu 180
185 190 Lys Thr Leu Ser Val Asp Lys Asp
Glu Leu Ser Leu Asp Gln Ala Ser 195 200
205 Val Leu Lys Asp Ser Lys Ile Glu Ala Val Glu Pro Glu
Lys Ser Ser 210 215 220
Ile Ala Lys Ile Ala Asn Leu Gln Pro Glu Val Asp Pro Lys Ala Glu 225
230 235 240 Leu Tyr Tyr Tyr
Pro Lys Gly Asp Asp Leu Leu Leu Val Tyr Val Thr 245
250 255 Glu Val Asn Val Leu Glu Pro Ala Pro
Leu Arg Thr Arg Tyr Ile Ile 260 265
270 Asp Ala Asn Asp Gly Ser Ile Val Phe Gln Tyr Asp Ile Ile
Asn Glu 275 280 285
Ala Thr Gly Thr Gly Lys Gly Val Leu Gly Asp Ser Lys Ser Phe Thr 290
295 300 Thr Thr Ala Ser Gly
Ser Ser Tyr Gln Leu Lys Asp Thr Thr Arg Gly 305 310
315 320 Asn Gly Ile Val Thr Tyr Thr Ala Ser Asn
Arg Gln Ser Ile Pro Gly 325 330
335 Thr Ile Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro Ala Gly
Val 340 345 350 Asp
Ala His Ala Tyr Ala Ala Lys Thr Tyr Asp Tyr Tyr Lys Ala Lys 355
360 365 Phe Gly Arg Asn Ser Ile
Asp Gly Arg Gly Leu Gln Leu Arg Ser Thr 370 375
380 Val His Tyr Gly Ser Arg Tyr Asn Asn Ala Phe
Trp Asn Gly Ser Gln 385 390 395
400 Met Thr Tyr Gly Asp Gly Asp Gly Ser Thr Phe Ile Ala Phe Ser Gly
405 410 415 Asp Pro
Asp Val Val Gly His Glu Leu Thr His Gly Val Thr Glu Tyr 420
425 430 Thr Ser Asn Leu Glu Tyr Tyr
Gly Glu Ser Gly Ala Leu Asn Glu Ala 435 440
445 Phe Ser Asp Val Ile Gly Asn Asp Ile Gln Arg Lys
Asn Trp Leu Val 450 455 460
Gly Asp Asp Ile Tyr Thr Pro Asn Ile Ala Gly Asp Ala Leu Arg Ser 465
470 475 480 Met Ser Asn
Pro Thr Leu Tyr Asp Gln Pro Asp His Tyr Ser Asn Leu 485
490 495 Tyr Arg Gly Ser Ser Asp Asn Gly
Gly Val His Thr Asn Ser Gly Ile 500 505
510 Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Asn
Phe His Gly 515 520 525
Val Thr Val Asn Gly Ile Gly Arg Asp Ala Ala Val Gln Ile Tyr Tyr 530
535 540 Ser Ala Phe Thr
Asn Tyr Leu Thr Ser Ser Ser Asp Phe Ser Asn Ala 545 550
555 560 Arg Ala Ala Val Ile Gln Ala Ala Lys
Asp Leu Tyr Gly Ala Asn Ser 565 570
575 Ala Glu Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala Val Gly
Val Asn 580 585 590
28304PRTPaenibacillus polymyxa SC2misc_feature(1)..(304)amino acid
sequence of the predicted mature form of PpoPro1 28Ala Thr Gly Thr
Gly Lys Gly Val Leu Gly Asp Ser Lys Ser Phe Thr 1 5
10 15 Thr Thr Ala Ser Gly Ser Ser Tyr Gln
Leu Lys Asp Thr Thr Arg Gly 20 25
30 Asn Gly Ile Val Thr Tyr Thr Ala Ser Asn Arg Gln Ser Ile
Pro Gly 35 40 45
Thr Ile Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro Ala Gly Val 50
55 60 Asp Ala His Ala Tyr
Ala Ala Lys Thr Tyr Asp Tyr Tyr Lys Ala Lys 65 70
75 80 Phe Gly Arg Asn Ser Ile Asp Gly Arg Gly
Leu Gln Leu Arg Ser Thr 85 90
95 Val His Tyr Gly Ser Arg Tyr Asn Asn Ala Phe Trp Asn Gly Ser
Gln 100 105 110 Met
Thr Tyr Gly Asp Gly Asp Gly Ser Thr Phe Ile Ala Phe Ser Gly 115
120 125 Asp Pro Asp Val Val Gly
His Glu Leu Thr His Gly Val Thr Glu Tyr 130 135
140 Thr Ser Asn Leu Glu Tyr Tyr Gly Glu Ser Gly
Ala Leu Asn Glu Ala 145 150 155
160 Phe Ser Asp Val Ile Gly Asn Asp Ile Gln Arg Lys Asn Trp Leu Val
165 170 175 Gly Asp
Asp Ile Tyr Thr Pro Asn Ile Ala Gly Asp Ala Leu Arg Ser 180
185 190 Met Ser Asn Pro Thr Leu Tyr
Asp Gln Pro Asp His Tyr Ser Asn Leu 195 200
205 Tyr Arg Gly Ser Ser Asp Asn Gly Gly Val His Thr
Asn Ser Gly Ile 210 215 220
Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Asn Phe His Gly 225
230 235 240 Val Thr Val
Asn Gly Ile Gly Arg Asp Ala Ala Val Gln Ile Tyr Tyr 245
250 255 Ser Ala Phe Thr Asn Tyr Leu Thr
Ser Ser Ser Asp Phe Ser Asn Ala 260 265
270 Arg Ala Ala Val Ile Gln Ala Ala Lys Asp Leu Tyr Gly
Ala Asn Ser 275 280 285
Ala Glu Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala Val Gly Val Asn 290
295 300
291800DNAArtificial SequenceSynthetic nucleotide sequence of the
synthesized PpoPro1 gene in plasmid pGX138(AprE-PpoPro1) 29gtgagaagca
aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat ctttacgatg 60gcgttcagca
acatgagcgc gcaggctgct ggaaaagaat catcagtgtc aggaccggct 120cagcttacac
cgacatttca cgcagaacaa tggaaggctc cgacgtcagt ttcaggagac 180gacatcgtgt
ggagctacct gaatagacag aagaaaagcc tgctgggagt ggatagcagc 240agcgtcagag
agcagttcag aatcgttgac agaacgagcg acaaaagcgg agtcagccat 300tatagactga
agcagtacgt gaatggcatc ccggtttatg gcgcagagca gacaattcat 360gttggcaaga
gcggagaagt cacaagctat ctgggcgctg tggtcaatga agatcaacaa 420gccgaggcta
cacagggaac aacgccgaaa attagcgcct cagaggcagt ctacacggcg 480tacaaagaag
cggctgcaag aatcgaagcc ctgccgacat cagacgatac aatttcaaaa 540gatgcggagg
agccgagctc agttagcaag gatacatacg cggaagccgc aaacaatgag 600aaaacactga
gcgtggacaa ggacgagctg tcacttgatc aggctagcgt ccttaaagac 660agcaagatcg
aggccgttga gcctgaaaag tcatcaattg cgaaaatcgc caatctgcaa 720cctgaagtcg
acccgaaggc ggaactgtac tactacccga aaggcgatga cctgcttctg 780gtgtacgtca
cggaagtgaa cgtcctggaa ccggcaccgc tgagaacaag atacatcatc 840gacgcgaacg
acggaagcat cgtcttccag tatgacatta tcaacgaagc aacgggaacg 900ggcaaaggcg
ttcttggaga ctcaaagagc ttcacgacaa cggcttcagg aagcagctac 960cagctgaaag
acacgacgag aggaaacgga atcgtcacat atacggcgtc aaacagacaa 1020agcatccctg
gcacaatcct gacggatgct gacaacgttt ggaatgatcc ggctggcgtg 1080gatgcccatg
cttatgcggc aaaaacgtat gactattaca aggcgaagtt cggcagaaat 1140tcaatcgatg
gcagaggact gcagcttaga agcacggtgc actacggatc aagatataac 1200aatgccttct
ggaacggcag ccagatgaca tacggagacg gagatggaag cacatttatt 1260gcattcagcg
gcgaccctga tgtggttggc catgagctga cgcatggcgt tacagaatat 1320acgagcaatc
ttgaatacta cggcgagtca ggcgctctga acgaggcatt tagcgatgtt 1380atcggcaatg
acatccagag aaaaaactgg ctggtgggcg acgatattta cacgcctaat 1440atcgctggcg
atgcccttag atcaatgtca aacccgacgc tgtatgatca gcctgaccac 1500tactcaaacc
tgtatagagg ctcatcagat aacggaggcg tccatacgaa tagcggcatc 1560attaacaagg
catattatct tctggcccag ggcggcaatt ttcatggagt gacggttaat 1620ggaattggaa
gagacgcagc cgtccaaatc tactacagcg ctttcacgaa ctaccttaca 1680tcaagctcag
actttagcaa tgccagagct gctgttatcc aggcagcgaa ggatctttac 1740ggcgccaact
cagccgaagc tacggccgca gctaaatcat ttgatgcagt gggcgttaat
180030600PRTArtificial SequenceSynthetic amino acid sequence of the
PpoPro1 precursor protein expressed from plasmid
pGX138(AprE-PpoPro1) 30Met Arg Ser Lys Lys Leu Trp Ile Ser Leu Leu Phe
Ala Leu Thr Leu 1 5 10
15 Ile Phe Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala Ala Gly Lys
20 25 30 Glu Ser Ser
Val Ser Gly Pro Ala Gln Leu Thr Pro Thr Phe His Ala 35
40 45 Glu Gln Trp Lys Ala Pro Thr Ser
Val Ser Gly Asp Asp Ile Val Trp 50 55
60 Ser Tyr Leu Asn Arg Gln Lys Lys Ser Leu Leu Gly Val
Asp Ser Ser 65 70 75
80 Ser Val Arg Glu Gln Phe Arg Ile Val Asp Arg Thr Ser Asp Lys Ser
85 90 95 Gly Val Ser His
Tyr Arg Leu Lys Gln Tyr Val Asn Gly Ile Pro Val 100
105 110 Tyr Gly Ala Glu Gln Thr Ile His Val
Gly Lys Ser Gly Glu Val Thr 115 120
125 Ser Tyr Leu Gly Ala Val Val Asn Glu Asp Gln Gln Ala Glu
Ala Thr 130 135 140
Gln Gly Thr Thr Pro Lys Ile Ser Ala Ser Glu Ala Val Tyr Thr Ala 145
150 155 160 Tyr Lys Glu Ala Ala
Ala Arg Ile Glu Ala Leu Pro Thr Ser Asp Asp 165
170 175 Thr Ile Ser Lys Asp Ala Glu Glu Pro Ser
Ser Val Ser Lys Asp Thr 180 185
190 Tyr Ala Glu Ala Ala Asn Asn Glu Lys Thr Leu Ser Val Asp Lys
Asp 195 200 205 Glu
Leu Ser Leu Asp Gln Ala Ser Val Leu Lys Asp Ser Lys Ile Glu 210
215 220 Ala Val Glu Pro Glu Lys
Ser Ser Ile Ala Lys Ile Ala Asn Leu Gln 225 230
235 240 Pro Glu Val Asp Pro Lys Ala Glu Leu Tyr Tyr
Tyr Pro Lys Gly Asp 245 250
255 Asp Leu Leu Leu Val Tyr Val Thr Glu Val Asn Val Leu Glu Pro Ala
260 265 270 Pro Leu
Arg Thr Arg Tyr Ile Ile Asp Ala Asn Asp Gly Ser Ile Val 275
280 285 Phe Gln Tyr Asp Ile Ile Asn
Glu Ala Thr Gly Thr Gly Lys Gly Val 290 295
300 Leu Gly Asp Ser Lys Ser Phe Thr Thr Thr Ala Ser
Gly Ser Ser Tyr 305 310 315
320 Gln Leu Lys Asp Thr Thr Arg Gly Asn Gly Ile Val Thr Tyr Thr Ala
325 330 335 Ser Asn Arg
Gln Ser Ile Pro Gly Thr Ile Leu Thr Asp Ala Asp Asn 340
345 350 Val Trp Asn Asp Pro Ala Gly Val
Asp Ala His Ala Tyr Ala Ala Lys 355 360
365 Thr Tyr Asp Tyr Tyr Lys Ala Lys Phe Gly Arg Asn Ser
Ile Asp Gly 370 375 380
Arg Gly Leu Gln Leu Arg Ser Thr Val His Tyr Gly Ser Arg Tyr Asn 385
390 395 400 Asn Ala Phe Trp
Asn Gly Ser Gln Met Thr Tyr Gly Asp Gly Asp Gly 405
410 415 Ser Thr Phe Ile Ala Phe Ser Gly Asp
Pro Asp Val Val Gly His Glu 420 425
430 Leu Thr His Gly Val Thr Glu Tyr Thr Ser Asn Leu Glu Tyr
Tyr Gly 435 440 445
Glu Ser Gly Ala Leu Asn Glu Ala Phe Ser Asp Val Ile Gly Asn Asp 450
455 460 Ile Gln Arg Lys Asn
Trp Leu Val Gly Asp Asp Ile Tyr Thr Pro Asn 465 470
475 480 Ile Ala Gly Asp Ala Leu Arg Ser Met Ser
Asn Pro Thr Leu Tyr Asp 485 490
495 Gln Pro Asp His Tyr Ser Asn Leu Tyr Arg Gly Ser Ser Asp Asn
Gly 500 505 510 Gly
Val His Thr Asn Ser Gly Ile Ile Asn Lys Ala Tyr Tyr Leu Leu 515
520 525 Ala Gln Gly Gly Asn Phe
His Gly Val Thr Val Asn Gly Ile Gly Arg 530 535
540 Asp Ala Ala Val Gln Ile Tyr Tyr Ser Ala Phe
Thr Asn Tyr Leu Thr 545 550 555
560 Ser Ser Ser Asp Phe Ser Asn Ala Arg Ala Ala Val Ile Gln Ala Ala
565 570 575 Lys Asp
Leu Tyr Gly Ala Asn Ser Ala Glu Ala Thr Ala Ala Ala Lys 580
585 590 Ser Phe Asp Ala Val Gly Val
Asn 595 600 311641DNAPaenibacillus
hunanensismisc_feature(1)..(1641)nucleotide sequence of the PhuPro1 gene
isolated from Paenibacillus hunanensis 31ttgaaaaaaa cagttggtct
tttacttgca ggtagcttgc tcgttggtgc tacaacgtcc 60gctttcgcag cagaagcaaa
tgatctggca ccactcggtg attacacgcc aaaattgatt 120acgcaagcaa caggcatcac
tggcgctagt ggcgatgcta aagtatggaa gttcctggag 180aagcaaaaac gtaccatcgt
aaccgatgat gcagcttctg ctgatgtgaa ggaattgttt 240gagatcacaa aacgtcaatc
cgattctcaa accggtacag agcactatcg cctgaaccaa 300acctttaaag gcatcccagt
ctatggcgca gagcaaacac tgcactttga caaatccggc 360aatgtatctc tgtacatggg
tcaggttgtt gaggatgtgt ccgctaaact ggaagcttcc 420gattccaaaa aaggcgtaac
tgaggatgta tacgcttcgg atacgaaaaa tgatctggta 480acaccagaaa tcagcgcttc
tcaagccatc tcgattgctg aaaaggatgc agcttccaaa 540atcggctccc tcggcgaagc
acaaaaaacg ccagaagcga agctgtatat ctacgctcct 600gaggatcaag cagcacgtct
ggcttatgtg acagaagtaa acgtactgga gccatctccg 660ctgcgtactc gctattttgt
agatgcaaaa acaggttcga tcctgttcca atatgatctg 720attgagcatg caacaggtac
aggtaaaggg gtactgggtg ataccaagtc cttcactgta 780ggtacttccg gttcttccta
tgtgatgact gatagcacgc gtggaaaagg tatccaaacc 840tacacggcgt ctaaccgcac
atcactgcca ggtagcactg taacgagcag cagcagcaca 900tttaacgatc cagcatctgt
cgatgcccat gcgtatgcac aaaaagtata tgatttctac 960aaatccaact ttaaccgcaa
cagcatcgac ggtaatggtc tggctatccg ctccactacg 1020cactattcca cacgttataa
caatgcgttc tggaatggtt cccaaatggt atacggtgat 1080ggcgatggtt cgcaattcat
cgcattctcc ggcgaccttg acgtagtagg tcacgagctg 1140acacacggtg taaccgagta
cacagcgaac ctggaatact atggtcaatc cggtgcactg 1200aacgaatcca tttcggatat
ctttggtaac acaatcgaag gtaaaaactg gatggtaggc 1260gatgcgatct acacaccagg
cgtatccggc gatgctcttc gctacatgga tgatccaaca 1320aaaggtggac aaccagcgcg
tatggcagat tacaacaaca caagcgctga taatggcggt 1380gtacacacaa acagtggtat
cccgaataaa gcatactact tgctggcaca gggtggcaca 1440tttggcggtg taaatgtaac
aggtatcggt cgctcgcaag cgatccagat cgtttaccgt 1500gcactaacat actacctgac
atccacatct aacttctcga actaccgttc tgcaatggtg 1560caagcatcta cagacctgta
cggtgcaaac tctacacaaa caacagcggt gaaaaactcg 1620ctgagcgcag taggcattaa c
164132547PRTPaenibacillus
hunanensismisc_feature(1)..(547)amino acid sequence of the PhuPro1
precursor protein 32Met Lys Lys Thr Val Gly Leu Leu Leu Ala Gly Ser
Leu Leu Val Gly 1 5 10
15 Ala Thr Thr Ser Ala Phe Ala Ala Glu Ala Asn Asp Leu Ala Pro Leu
20 25 30 Gly Asp Tyr
Thr Pro Lys Leu Ile Thr Gln Ala Thr Gly Ile Thr Gly 35
40 45 Ala Ser Gly Asp Ala Lys Val Trp
Lys Phe Leu Glu Lys Gln Lys Arg 50 55
60 Thr Ile Val Thr Asp Asp Ala Ala Ser Ala Asp Val Lys
Glu Leu Phe 65 70 75
80 Glu Ile Thr Lys Arg Gln Ser Asp Ser Gln Thr Gly Thr Glu His Tyr
85 90 95 Arg Leu Asn Gln
Thr Phe Lys Gly Ile Pro Val Tyr Gly Ala Glu Gln 100
105 110 Thr Leu His Phe Asp Lys Ser Gly Asn
Val Ser Leu Tyr Met Gly Gln 115 120
125 Val Val Glu Asp Val Ser Ala Lys Leu Glu Ala Ser Asp Ser
Lys Lys 130 135 140
Gly Val Thr Glu Asp Val Tyr Ala Ser Asp Thr Lys Asn Asp Leu Val 145
150 155 160 Thr Pro Glu Ile Ser
Ala Ser Gln Ala Ile Ser Ile Ala Glu Lys Asp 165
170 175 Ala Ala Ser Lys Ile Gly Ser Leu Gly Glu
Ala Gln Lys Thr Pro Glu 180 185
190 Ala Lys Leu Tyr Ile Tyr Ala Pro Glu Asp Gln Ala Ala Arg Leu
Ala 195 200 205 Tyr
Val Thr Glu Val Asn Val Leu Glu Pro Ser Pro Leu Arg Thr Arg 210
215 220 Tyr Phe Val Asp Ala Lys
Thr Gly Ser Ile Leu Phe Gln Tyr Asp Leu 225 230
235 240 Ile Glu His Ala Thr Gly Thr Gly Lys Gly Val
Leu Gly Asp Thr Lys 245 250
255 Ser Phe Thr Val Gly Thr Ser Gly Ser Ser Tyr Val Met Thr Asp Ser
260 265 270 Thr Arg
Gly Lys Gly Ile Gln Thr Tyr Thr Ala Ser Asn Arg Thr Ser 275
280 285 Leu Pro Gly Ser Thr Val Thr
Ser Ser Ser Ser Thr Phe Asn Asp Pro 290 295
300 Ala Ser Val Asp Ala His Ala Tyr Ala Gln Lys Val
Tyr Asp Phe Tyr 305 310 315
320 Lys Ser Asn Phe Asn Arg Asn Ser Ile Asp Gly Asn Gly Leu Ala Ile
325 330 335 Arg Ser Thr
Thr His Tyr Ser Thr Arg Tyr Asn Asn Ala Phe Trp Asn 340
345 350 Gly Ser Gln Met Val Tyr Gly Asp
Gly Asp Gly Ser Gln Phe Ile Ala 355 360
365 Phe Ser Gly Asp Leu Asp Val Val Gly His Glu Leu Thr
His Gly Val 370 375 380
Thr Glu Tyr Thr Ala Asn Leu Glu Tyr Tyr Gly Gln Ser Gly Ala Leu 385
390 395 400 Asn Glu Ser Ile
Ser Asp Ile Phe Gly Asn Thr Ile Glu Gly Lys Asn 405
410 415 Trp Met Val Gly Asp Ala Ile Tyr Thr
Pro Gly Val Ser Gly Asp Ala 420 425
430 Leu Arg Tyr Met Asp Asp Pro Thr Lys Gly Gly Gln Pro Ala
Arg Met 435 440 445
Ala Asp Tyr Asn Asn Thr Ser Ala Asp Asn Gly Gly Val His Thr Asn 450
455 460 Ser Gly Ile Pro Asn
Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Thr 465 470
475 480 Phe Gly Gly Val Asn Val Thr Gly Ile Gly
Arg Ser Gln Ala Ile Gln 485 490
495 Ile Val Tyr Arg Ala Leu Thr Tyr Tyr Leu Thr Ser Thr Ser Asn
Phe 500 505 510 Ser
Asn Tyr Arg Ser Ala Met Val Gln Ala Ser Thr Asp Leu Tyr Gly 515
520 525 Ala Asn Ser Thr Gln Thr
Thr Ala Val Lys Asn Ser Leu Ser Ala Val 530 535
540 Gly Ile Asn 545
33304PRTPaenibacillus hunanensismisc_feature(1)..(304)amino acid sequence
of the predicted mature form of PhuPro1 33Ala Thr Gly Thr Gly Lys
Gly Val Leu Gly Asp Thr Lys Ser Phe Thr 1 5
10 15 Val Gly Thr Ser Gly Ser Ser Tyr Val Met Thr
Asp Ser Thr Arg Gly 20 25
30 Lys Gly Ile Gln Thr Tyr Thr Ala Ser Asn Arg Thr Ser Leu Pro
Gly 35 40 45 Ser
Thr Val Thr Ser Ser Ser Ser Thr Phe Asn Asp Pro Ala Ser Val 50
55 60 Asp Ala His Ala Tyr Ala
Gln Lys Val Tyr Asp Phe Tyr Lys Ser Asn 65 70
75 80 Phe Asn Arg Asn Ser Ile Asp Gly Asn Gly Leu
Ala Ile Arg Ser Thr 85 90
95 Thr His Tyr Ser Thr Arg Tyr Asn Asn Ala Phe Trp Asn Gly Ser Gln
100 105 110 Met Val
Tyr Gly Asp Gly Asp Gly Ser Gln Phe Ile Ala Phe Ser Gly 115
120 125 Asp Leu Asp Val Val Gly His
Glu Leu Thr His Gly Val Thr Glu Tyr 130 135
140 Thr Ala Asn Leu Glu Tyr Tyr Gly Gln Ser Gly Ala
Leu Asn Glu Ser 145 150 155
160 Ile Ser Asp Ile Phe Gly Asn Thr Ile Glu Gly Lys Asn Trp Met Val
165 170 175 Gly Asp Ala
Ile Tyr Thr Pro Gly Val Ser Gly Asp Ala Leu Arg Tyr 180
185 190 Met Asp Asp Pro Thr Lys Gly Gly
Gln Pro Ala Arg Met Ala Asp Tyr 195 200
205 Asn Asn Thr Ser Ala Asp Asn Gly Gly Val His Thr Asn
Ser Gly Ile 210 215 220
Pro Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Thr Phe Gly Gly 225
230 235 240 Val Asn Val Thr
Gly Ile Gly Arg Ser Gln Ala Ile Gln Ile Val Tyr 245
250 255 Arg Ala Leu Thr Tyr Tyr Leu Thr Ser
Thr Ser Asn Phe Ser Asn Tyr 260 265
270 Arg Ser Ala Met Val Gln Ala Ser Thr Asp Leu Tyr Gly Ala
Asn Ser 275 280 285
Thr Gln Thr Thr Ala Val Lys Asn Ser Leu Ser Ala Val Gly Ile Asn 290
295 300 341671DNAArtificial
SequenceSynthetic nucleotide sequence of the synthesized PhuPro1
gene in plasmid pGX149(AprE- PhuPro1) 34gtgagaagca aaaaattgtg gatcagcttg
ttgtttgcgt taacgttaat ctttacgatg 60gcgttcagca acatgagcgc gcaggctgct
ggaaaagcag aagctaatga tcttgccccg 120cttggcgatt atacaccgaa gcttattaca
caggcaacgg gaattacagg cgcatcaggc 180gatgcgaagg tgtggaagtt cctggagaag
cagaagagaa cgattgtcac ggacgacgcc 240gcaagcgcgg atgtcaagga gctgttcgag
atcacgaaga gacagagcga tagccagacg 300ggaacggagc attacagact gaaccagacg
ttcaagggca ttccggtcta cggagctgaa 360caaacgctgc attttgataa aagcggcaac
gtctcactgt acatgggcca agtcgttgag 420gacgttagcg ccaaacttga ggctagcgac
agcaagaaag gcgtcacaga agatgtctac 480gcgtcagaca cgaaaaacga cctggttaca
ccggaaatct cagcttcaca ggccatctca 540attgcagaga aagacgcagc gtcaaaaatc
ggctcactgg gcgaggctca gaaaacgccg 600gaggcgaaac tttacatcta cgcccctgag
gaccaggctg cgagactggc ttacgtgaca 660gaagttaatg tgctggagcc gtcaccgctt
agaacgagat atttcgtgga cgcaaagacg 720ggcagcattc tgtttcagta cgatcttatc
gaacacgcga caggcacagg aaagggagtt 780ctgggagaca caaaaagctt cacggttggc
acgtcaggca gcagctacgt gatgacagac 840agcacgagag gcaagggcat tcaaacgtat
acagcgagca acagaacaag cctgccggga 900agcacagtca cgagctcatc atcaacgttt
aatgacccgg cctcagtgga tgctcacgca 960tacgcgcaga aagtgtacga cttctacaaa
agcaacttca atagaaacag catcgacgga 1020aacggccttg cgatcagaag cacgacgcac
tacagcacaa gatacaacaa cgccttctgg 1080aacggcagcc aaatggttta cggcgatggc
gacggatcac agtttatcgc atttagcgga 1140gacctggacg tcgttggcca tgagctgaca
catggcgtta cggagtacac agcaaacctg 1200gaatactatg gccagtcagg cgcccttaac
gagagcatca gcgacatttt tggcaatacg 1260atcgaaggaa agaactggat ggtcggcgac
gcaatctaca caccgggcgt ttcaggcgat 1320gcactgagat atatggacga cccgacaaag
ggcggacagc cggccagaat ggcggattac 1380aataatacgt cagcagataa cggcggcgtg
catacaaata gcggcatccc taacaaagca 1440tattacctgc ttgcgcaagg aggaacattt
ggcggcgtga atgttacggg cattggcaga 1500tcacaagcga ttcagatcgt ttacagagcg
ctgacgtact accttacgag cacgagcaat 1560tttagcaact acagaagcgc aatggtgcag
gcaagcacgg atctgtatgg cgcaaattca 1620acacaaacga cggcggtcaa gaatagcctt
tcagcagtgg gcattaacta a 167135556PRTArtificial
SequenceSynthetic amino acid sequence of the PhuPro1 precursor
protein expressed from plasmid pGX149(AprE- PhuPro1) 35Met Arg Ser Lys
Lys Leu Trp Ile Ser Leu Leu Phe Ala Leu Thr Leu 1 5
10 15 Ile Phe Thr Met Ala Phe Ser Asn Met
Ser Ala Gln Ala Ala Gly Lys 20 25
30 Ala Glu Ala Asn Asp Leu Ala Pro Leu Gly Asp Tyr Thr Pro
Lys Leu 35 40 45
Ile Thr Gln Ala Thr Gly Ile Thr Gly Ala Ser Gly Asp Ala Lys Val 50
55 60 Trp Lys Phe Leu Glu
Lys Gln Lys Arg Thr Ile Val Thr Asp Asp Ala 65 70
75 80 Ala Ser Ala Asp Val Lys Glu Leu Phe Glu
Ile Thr Lys Arg Gln Ser 85 90
95 Asp Ser Gln Thr Gly Thr Glu His Tyr Arg Leu Asn Gln Thr Phe
Lys 100 105 110 Gly
Ile Pro Val Tyr Gly Ala Glu Gln Thr Leu His Phe Asp Lys Ser 115
120 125 Gly Asn Val Ser Leu Tyr
Met Gly Gln Val Val Glu Asp Val Ser Ala 130 135
140 Lys Leu Glu Ala Ser Asp Ser Lys Lys Gly Val
Thr Glu Asp Val Tyr 145 150 155
160 Ala Ser Asp Thr Lys Asn Asp Leu Val Thr Pro Glu Ile Ser Ala Ser
165 170 175 Gln Ala
Ile Ser Ile Ala Glu Lys Asp Ala Ala Ser Lys Ile Gly Ser 180
185 190 Leu Gly Glu Ala Gln Lys Thr
Pro Glu Ala Lys Leu Tyr Ile Tyr Ala 195 200
205 Pro Glu Asp Gln Ala Ala Arg Leu Ala Tyr Val Thr
Glu Val Asn Val 210 215 220
Leu Glu Pro Ser Pro Leu Arg Thr Arg Tyr Phe Val Asp Ala Lys Thr 225
230 235 240 Gly Ser Ile
Leu Phe Gln Tyr Asp Leu Ile Glu His Ala Thr Gly Thr 245
250 255 Gly Lys Gly Val Leu Gly Asp Thr
Lys Ser Phe Thr Val Gly Thr Ser 260 265
270 Gly Ser Ser Tyr Val Met Thr Asp Ser Thr Arg Gly Lys
Gly Ile Gln 275 280 285
Thr Tyr Thr Ala Ser Asn Arg Thr Ser Leu Pro Gly Ser Thr Val Thr 290
295 300 Ser Ser Ser Ser
Thr Phe Asn Asp Pro Ala Ser Val Asp Ala His Ala 305 310
315 320 Tyr Ala Gln Lys Val Tyr Asp Phe Tyr
Lys Ser Asn Phe Asn Arg Asn 325 330
335 Ser Ile Asp Gly Asn Gly Leu Ala Ile Arg Ser Thr Thr His
Tyr Ser 340 345 350
Thr Arg Tyr Asn Asn Ala Phe Trp Asn Gly Ser Gln Met Val Tyr Gly
355 360 365 Asp Gly Asp Gly
Ser Gln Phe Ile Ala Phe Ser Gly Asp Leu Asp Val 370
375 380 Val Gly His Glu Leu Thr His Gly
Val Thr Glu Tyr Thr Ala Asn Leu 385 390
395 400 Glu Tyr Tyr Gly Gln Ser Gly Ala Leu Asn Glu Ser
Ile Ser Asp Ile 405 410
415 Phe Gly Asn Thr Ile Glu Gly Lys Asn Trp Met Val Gly Asp Ala Ile
420 425 430 Tyr Thr Pro
Gly Val Ser Gly Asp Ala Leu Arg Tyr Met Asp Asp Pro 435
440 445 Thr Lys Gly Gly Gln Pro Ala Arg
Met Ala Asp Tyr Asn Asn Thr Ser 450 455
460 Ala Asp Asn Gly Gly Val His Thr Asn Ser Gly Ile Pro
Asn Lys Ala 465 470 475
480 Tyr Tyr Leu Leu Ala Gln Gly Gly Thr Phe Gly Gly Val Asn Val Thr
485 490 495 Gly Ile Gly Arg
Ser Gln Ala Ile Gln Ile Val Tyr Arg Ala Leu Thr 500
505 510 Tyr Tyr Leu Thr Ser Thr Ser Asn Phe
Ser Asn Tyr Arg Ser Ala Met 515 520
525 Val Gln Ala Ser Thr Asp Leu Tyr Gly Ala Asn Ser Thr Gln
Thr Thr 530 535 540
Ala Val Lys Asn Ser Leu Ser Ala Val Gly Ile Asn 545 550
555 361563DNAPaenibacillus
amylolyticusmisc_feature(1)..(1563)nucleotide sequence of the PamPro1
gene isolated from Paenibacillus amylolyticus 36atgaaattcg
ccaaagttat gccaacaatt cttggaggag ctcttttgct cgcttccgta 60tcctctgcta
ctgcagctcc agtgtctgat caatccattc cacttcaggc cccttatgcc 120tctgaggggg
gtattccatt gaacagtgga acagatgaca ctatctttaa ttatcttgga 180cagcaggaac
aatttctgaa ttccgatgtg aaatcccagc tcaaaattgt caaaagaaac 240acagatacat
ctggcgtaag acacttccgc ctgaaacagt atattaaagg tatcccggtt 300tatggtgcag
aacagacggt ccacctggac aaaaccggag ccgtgagctc cgcacttggc 360gatcttccac
cgattgaaga gcaggccatt ccgaatgatg gtgtagccga gatcagcgga 420gaagacgcga
tccagattgc aaccgaagaa gcaacctccc ggattggaga gcttggtgcc 480gcggaaatca
cgcctcaagc tgaattgaac atctatcatc atgaagaaga tggtcagaca 540tatctggttt
acattacgga agtaaacgta ctggaacctg cccctctacg gaccaaatat 600ttcattaacg
cagtggatgg cagtatcgta tcccagtttg acctcattaa cttcgctact 660ggaacaggta
caggtgtact cggtgatacc aaaaccctga caaccaccca atccggcagc 720accttccaac
tgaaagacac cactcgtggc aatggcatcc aaacgtatac ggcaaacaat 780ggctcctcac
tgcctggtag cttgcttaca gattcggata atgtatggac cgatcgtgca 840ggtgtagatg
ctcatgctca tgccgctgct acgtatgatt tctacaaaaa caaattcaac 900cgtaacggta
ttaatggtaa cggattgttg atcagatcaa ccgtgcacta cggctccaat 960tacaataacg
ccttctggaa cggggcacag attgtctttg gtgacggaga tggaacgatg 1020ttccgatccc
tgtctggtga tctggatgtt gtgggtcatg aattgacgca tggtgttatt 1080gaatatacag
ccaatctgga atatcgcaat gaaccaggtg cactcaatga agcctttgcc 1140gatattttcg
gtaatacgat ccaaagcaaa aactggctgc tcggtgatga tatctacaca 1200cctaacactc
caggagatgc gctgcgctcc ctctccaacc ctacattgta tggtcaacct 1260gacaaataca
gcgatcgcta cacaggctca caggacaacg gcggtgtcca tatcaacagt 1320ggtatcatca
ataaagccta tttccttgct gctcaaggcg gaacacataa tggtgtgact 1380gttaccggaa
tcggccggga taaagcgatc cagattttct acagcacact ggtgaactac 1440ctgacaccaa
cgtccaaatt tgccgctgcc aaaacagcta ccattcaagc agccaaagat 1500ctgtacggag
caacttccgc tgaagctact gctattacca aagcatatca agctgtaggc 1560ctg
156337521PRTPaenibacillus amylolyticusmisc_feature(1)..(521)amino acid
sequence of the PamPro1 precursor protein 37Met Lys Phe Ala Lys Val
Met Pro Thr Ile Leu Gly Gly Ala Leu Leu 1 5
10 15 Leu Ala Ser Val Ser Ser Ala Thr Ala Ala Pro
Val Ser Asp Gln Ser 20 25
30 Ile Pro Leu Gln Ala Pro Tyr Ala Ser Glu Gly Gly Ile Pro Leu
Asn 35 40 45 Ser
Gly Thr Asp Asp Thr Ile Phe Asn Tyr Leu Gly Gln Gln Glu Gln 50
55 60 Phe Leu Asn Ser Asp Val
Lys Ser Gln Leu Lys Ile Val Lys Arg Asn 65 70
75 80 Thr Asp Thr Ser Gly Val Arg His Phe Arg Leu
Lys Gln Tyr Ile Lys 85 90
95 Gly Ile Pro Val Tyr Gly Ala Glu Gln Thr Val His Leu Asp Lys Thr
100 105 110 Gly Ala
Val Ser Ser Ala Leu Gly Asp Leu Pro Pro Ile Glu Glu Gln 115
120 125 Ala Ile Pro Asn Asp Gly Val
Ala Glu Ile Ser Gly Glu Asp Ala Ile 130 135
140 Gln Ile Ala Thr Glu Glu Ala Thr Ser Arg Ile Gly
Glu Leu Gly Ala 145 150 155
160 Ala Glu Ile Thr Pro Gln Ala Glu Leu Asn Ile Tyr His His Glu Glu
165 170 175 Asp Gly Gln
Thr Tyr Leu Val Tyr Ile Thr Glu Val Asn Val Leu Glu 180
185 190 Pro Ala Pro Leu Arg Thr Lys Tyr
Phe Ile Asn Ala Val Asp Gly Ser 195 200
205 Ile Val Ser Gln Phe Asp Leu Ile Asn Phe Ala Thr Gly
Thr Gly Thr 210 215 220
Gly Val Leu Gly Asp Thr Lys Thr Leu Thr Thr Thr Gln Ser Gly Ser 225
230 235 240 Thr Phe Gln Leu
Lys Asp Thr Thr Arg Gly Asn Gly Ile Gln Thr Tyr 245
250 255 Thr Ala Asn Asn Gly Ser Ser Leu Pro
Gly Ser Leu Leu Thr Asp Ser 260 265
270 Asp Asn Val Trp Thr Asp Arg Ala Gly Val Asp Ala His Ala
His Ala 275 280 285
Ala Ala Thr Tyr Asp Phe Tyr Lys Asn Lys Phe Asn Arg Asn Gly Ile 290
295 300 Asn Gly Asn Gly Leu
Leu Ile Arg Ser Thr Val His Tyr Gly Ser Asn 305 310
315 320 Tyr Asn Asn Ala Phe Trp Asn Gly Ala Gln
Ile Val Phe Gly Asp Gly 325 330
335 Asp Gly Thr Met Phe Arg Ser Leu Ser Gly Asp Leu Asp Val Val
Gly 340 345 350 His
Glu Leu Thr His Gly Val Ile Glu Tyr Thr Ala Asn Leu Glu Tyr 355
360 365 Arg Asn Glu Pro Gly Ala
Leu Asn Glu Ala Phe Ala Asp Ile Phe Gly 370 375
380 Asn Thr Ile Gln Ser Lys Asn Trp Leu Leu Gly
Asp Asp Ile Tyr Thr 385 390 395
400 Pro Asn Thr Pro Gly Asp Ala Leu Arg Ser Leu Ser Asn Pro Thr Leu
405 410 415 Tyr Gly
Gln Pro Asp Lys Tyr Ser Asp Arg Tyr Thr Gly Ser Gln Asp 420
425 430 Asn Gly Gly Val His Ile Asn
Ser Gly Ile Ile Asn Lys Ala Tyr Phe 435 440
445 Leu Ala Ala Gln Gly Gly Thr His Asn Gly Val Thr
Val Thr Gly Ile 450 455 460
Gly Arg Asp Lys Ala Ile Gln Ile Phe Tyr Ser Thr Leu Val Asn Tyr 465
470 475 480 Leu Thr Pro
Thr Ser Lys Phe Ala Ala Ala Lys Thr Ala Thr Ile Gln 485
490 495 Ala Ala Lys Asp Leu Tyr Gly Ala
Thr Ser Ala Glu Ala Thr Ala Ile 500 505
510 Thr Lys Ala Tyr Gln Ala Val Gly Leu 515
520 38303PRTPaenibacillus
amylolyticusmisc_feature(1)..(303)amino acid sequence of the predicted
mature form of PamPro1 38Ala Thr Gly Thr Gly Thr Gly Val Leu Gly Asp
Thr Lys Thr Leu Thr 1 5 10
15 Thr Thr Gln Ser Gly Ser Thr Phe Gln Leu Lys Asp Thr Thr Arg Gly
20 25 30 Asn Gly
Ile Gln Thr Tyr Thr Ala Asn Asn Gly Ser Ser Leu Pro Gly 35
40 45 Ser Leu Leu Thr Asp Ser Asp
Asn Val Trp Thr Asp Arg Ala Gly Val 50 55
60 Asp Ala His Ala His Ala Ala Ala Thr Tyr Asp Phe
Tyr Lys Asn Lys 65 70 75
80 Phe Asn Arg Asn Gly Ile Asn Gly Asn Gly Leu Leu Ile Arg Ser Thr
85 90 95 Val His Tyr
Gly Ser Asn Tyr Asn Asn Ala Phe Trp Asn Gly Ala Gln 100
105 110 Ile Val Phe Gly Asp Gly Asp Gly
Thr Met Phe Arg Ser Leu Ser Gly 115 120
125 Asp Leu Asp Val Val Gly His Glu Leu Thr His Gly Val
Ile Glu Tyr 130 135 140
Thr Ala Asn Leu Glu Tyr Arg Asn Glu Pro Gly Ala Leu Asn Glu Ala 145
150 155 160 Phe Ala Asp Ile
Phe Gly Asn Thr Ile Gln Ser Lys Asn Trp Leu Leu 165
170 175 Gly Asp Asp Ile Tyr Thr Pro Asn Thr
Pro Gly Asp Ala Leu Arg Ser 180 185
190 Leu Ser Asn Pro Thr Leu Tyr Gly Gln Pro Asp Lys Tyr Ser
Asp Arg 195 200 205
Tyr Thr Gly Ser Gln Asp Asn Gly Gly Val His Ile Asn Ser Gly Ile 210
215 220 Ile Asn Lys Ala Tyr
Phe Leu Ala Ala Gln Gly Gly Thr His Asn Gly 225 230
235 240 Val Thr Val Thr Gly Ile Gly Arg Asp Lys
Ala Ile Gln Ile Phe Tyr 245 250
255 Ser Thr Leu Val Asn Tyr Leu Thr Pro Thr Ser Lys Phe Ala Ala
Ala 260 265 270 Lys
Thr Ala Thr Ile Gln Ala Ala Lys Asp Leu Tyr Gly Ala Thr Ser 275
280 285 Ala Glu Ala Thr Ala Ile
Thr Lys Ala Tyr Gln Ala Val Gly Leu 290 295
300 391587DNAArtificial SequenceSynthetic nucleotide
sequence of the synthesized PamPro1 gene in plasmid pGX146(AprE-
PamPro1) 39gtgagaagca aaaaattgtg gatcagcttg ttgtttgcgt taacgttaat
ctttacgatg 60gcgttcagca acatgagcgc gcaggctgct ggaaaagctc cggttagcga
ccagtcaatc 120cctcttcaag caccgtatgc cagcgaagga ggcattccgc ttaacagcgg
cacggacgac 180acgattttca attacctggg ccaacaggag cagttcctga acagcgacgt
caagagccag 240ctgaagatcg tcaaaagaaa cacagacaca tcaggcgtga gacacttcag
actgaagcaa 300tacatcaagg gcatcccggt ttatggcgct gaacaaacgg ttcacctgga
caaaacaggc 360gcagtttcat cagcactggg agatctgccg ccgattgaag agcaagcaat
cccgaatgat 420ggagttgcgg aaattagcgg cgaggatgca atccaaatcg cgacggagga
ggctacatca 480agaattggag aacttggcgc agcggagatt acaccgcagg ctgaactgaa
catctatcac 540catgaggaag acggccagac gtacctggtt tacattacgg aagtgaacgt
gctggaaccg 600gcacctctga gaacaaagta ctttatcaac gcggttgacg gcagcatcgt
ctcacagttc 660gacctgatta acttcgccac gggaacagga acgggcgttc ttggagacac
aaagacgctg 720acgacgacgc agtcaggcag cacattccag ctgaaggaca caacaagagg
caacggcatc 780caaacgtaca cggcgaacaa tggatcatca ctgccgggct cactgctgac
ggattcagat 840aacgtgtgga cggatagagc tggcgttgac gcgcatgctc acgctgctgc
gacgtacgac 900ttctacaaga acaagttcaa cagaaacggc attaacggaa atggcctgct
gatcagaagc 960acggtgcatt atggctcaaa ctacaacaac gctttttgga acggcgcaca
gatcgtgttt 1020ggcgacggcg atggcacaat gtttagaagc ctgtcaggag acctggatgt
ggtgggccac 1080gaactgacgc acggcgtgat cgagtatacg gcgaaccttg aatatagaaa
cgagccggga 1140gcactgaatg aggcgttcgc ggacattttc ggcaacacaa tccagagcaa
aaactggctg 1200ctgggcgacg atatctatac accgaacaca ccgggcgatg cactgagatc
actgtcaaat 1260ccgacgctgt atggccaacc ggataagtac tcagacagat atacgggcag
ccaagacaat 1320ggcggcgttc acatcaactc aggcatcatc aacaaggctt acttccttgc
ggcccaagga 1380ggaacacata acggcgttac agttacaggc attggcagag acaaggcgat
ccagatcttt 1440tacagcacgc tggtgaacta cctgacacct acgtcaaagt ttgccgcagc
gaaaacagca 1500acaattcagg cggctaaaga cctgtacgga gcgacatcag ccgaggccac
agcaattaca 1560aaagcatatc aagcagttgg cctttaa
158740528PRTArtificial SequenceSynthetic amino acid sequence
of the PamPro1 precursor protein expressed from plasmid pGX146(AprE-
PamPro1) 40Met Arg Ser Lys Lys Leu Trp Ile Ser Leu Leu Phe Ala Leu Thr
Leu 1 5 10 15 Ile
Phe Thr Met Ala Phe Ser Asn Met Ser Ala Gln Ala Ala Gly Lys
20 25 30 Ala Pro Val Ser Asp
Gln Ser Ile Pro Leu Gln Ala Pro Tyr Ala Ser 35
40 45 Glu Gly Gly Ile Pro Leu Asn Ser Gly
Thr Asp Asp Thr Ile Phe Asn 50 55
60 Tyr Leu Gly Gln Gln Glu Gln Phe Leu Asn Ser Asp Val
Lys Ser Gln 65 70 75
80 Leu Lys Ile Val Lys Arg Asn Thr Asp Thr Ser Gly Val Arg His Phe
85 90 95 Arg Leu Lys Gln
Tyr Ile Lys Gly Ile Pro Val Tyr Gly Ala Glu Gln 100
105 110 Thr Val His Leu Asp Lys Thr Gly Ala
Val Ser Ser Ala Leu Gly Asp 115 120
125 Leu Pro Pro Ile Glu Glu Gln Ala Ile Pro Asn Asp Gly Val
Ala Glu 130 135 140
Ile Ser Gly Glu Asp Ala Ile Gln Ile Ala Thr Glu Glu Ala Thr Ser 145
150 155 160 Arg Ile Gly Glu Leu
Gly Ala Ala Glu Ile Thr Pro Gln Ala Glu Leu 165
170 175 Asn Ile Tyr His His Glu Glu Asp Gly Gln
Thr Tyr Leu Val Tyr Ile 180 185
190 Thr Glu Val Asn Val Leu Glu Pro Ala Pro Leu Arg Thr Lys Tyr
Phe 195 200 205 Ile
Asn Ala Val Asp Gly Ser Ile Val Ser Gln Phe Asp Leu Ile Asn 210
215 220 Phe Ala Thr Gly Thr Gly
Thr Gly Val Leu Gly Asp Thr Lys Thr Leu 225 230
235 240 Thr Thr Thr Gln Ser Gly Ser Thr Phe Gln Leu
Lys Asp Thr Thr Arg 245 250
255 Gly Asn Gly Ile Gln Thr Tyr Thr Ala Asn Asn Gly Ser Ser Leu Pro
260 265 270 Gly Ser
Leu Leu Thr Asp Ser Asp Asn Val Trp Thr Asp Arg Ala Gly 275
280 285 Val Asp Ala His Ala His Ala
Ala Ala Thr Tyr Asp Phe Tyr Lys Asn 290 295
300 Lys Phe Asn Arg Asn Gly Ile Asn Gly Asn Gly Leu
Leu Ile Arg Ser 305 310 315
320 Thr Val His Tyr Gly Ser Asn Tyr Asn Asn Ala Phe Trp Asn Gly Ala
325 330 335 Gln Ile Val
Phe Gly Asp Gly Asp Gly Thr Met Phe Arg Ser Leu Ser 340
345 350 Gly Asp Leu Asp Val Val Gly His
Glu Leu Thr His Gly Val Ile Glu 355 360
365 Tyr Thr Ala Asn Leu Glu Tyr Arg Asn Glu Pro Gly Ala
Leu Asn Glu 370 375 380
Ala Phe Ala Asp Ile Phe Gly Asn Thr Ile Gln Ser Lys Asn Trp Leu 385
390 395 400 Leu Gly Asp Asp
Ile Tyr Thr Pro Asn Thr Pro Gly Asp Ala Leu Arg 405
410 415 Ser Leu Ser Asn Pro Thr Leu Tyr Gly
Gln Pro Asp Lys Tyr Ser Asp 420 425
430 Arg Tyr Thr Gly Ser Gln Asp Asn Gly Gly Val His Ile Asn
Ser Gly 435 440 445
Ile Ile Asn Lys Ala Tyr Phe Leu Ala Ala Gln Gly Gly Thr His Asn 450
455 460 Gly Val Thr Val Thr
Gly Ile Gly Arg Asp Lys Ala Ile Gln Ile Phe 465 470
475 480 Tyr Ser Thr Leu Val Asn Tyr Leu Thr Pro
Thr Ser Lys Phe Ala Ala 485 490
495 Ala Lys Thr Ala Thr Ile Gln Ala Ala Lys Asp Leu Tyr Gly Ala
Thr 500 505 510 Ser
Ala Glu Ala Thr Ala Ile Thr Lys Ala Tyr Gln Ala Val Gly Leu 515
520 525 415PRTArtificial
SequenceSynthetic peptide 41His Glu Xaa Xaa His 1 5
425PRTArtificial SequenceSynthetic peptide 42His Asp Xaa Xaa His 1
5 434PRTArtificial SequenceSynthetic peptide 43Ala Ala Pro Phe 1
44306PRTPaenibacillus
sp.misc_feature(1)..(306)Paenibacillus_sp_Aloe-11 44Asn Glu Ala Thr Gly
Thr Gly Lys Gly Val Leu Gly Asp Thr Lys Thr 1 5
10 15 Phe Asn Thr Thr Ala Ser Gly Ser Ser Tyr
Gln Leu Arg Asp Thr Thr 20 25
30 Arg Gly Asn Gly Ile Val Thr Tyr Thr Ala Ser Asn Arg Gln Ser
Ile 35 40 45 Pro
Gly Thr Ile Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro Ala 50
55 60 Gly Val Asp Ala His Ala
Tyr Ala Ala Lys Thr Tyr Asp Tyr Tyr Lys 65 70
75 80 Glu Lys Phe Asn Arg Asn Ser Ile Asp Gly Arg
Gly Leu Gln Leu Arg 85 90
95 Ser Thr Val His Tyr Gly Asn Arg Tyr Asn Asn Ala Phe Trp Asn Gly
100 105 110 Ser Gln
Met Thr Tyr Gly Asp Gly Asp Gly Thr Thr Phe Ile Ala Phe 115
120 125 Ser Gly Asp Pro Asp Val Val
Gly His Glu Leu Thr His Gly Val Thr 130 135
140 Glu Tyr Thr Ser Asn Leu Glu Tyr Tyr Gly Glu Ser
Gly Ala Leu Asn 145 150 155
160 Glu Ala Phe Ser Asp Ile Ile Gly Asn Asp Ile Gln Arg Lys Asn Trp
165 170 175 Leu Val Gly
Asp Asp Ile Tyr Thr Pro Arg Ile Ala Gly Asp Ala Leu 180
185 190 Arg Ser Met Ser Asn Pro Thr Leu
Tyr Asp Gln Pro Asp His Tyr Ser 195 200
205 Asn Leu Tyr Arg Gly Ser Ser Asp Asn Gly Gly Val His
Thr Asn Ser 210 215 220
Gly Ile Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Thr Phe 225
230 235 240 His Gly Val Thr
Val Asn Gly Ile Gly Arg Asp Ala Ala Val Gln Ile 245
250 255 Tyr Tyr Ser Ala Phe Thr Asn Tyr Leu
Thr Ser Ser Ser Asp Phe Ser 260 265
270 Asn Ala Arg Asp Ala Val Val Gln Ala Ala Lys Asp Leu Tyr
Gly Ala 275 280 285
Ser Ser Ala Gln Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala Val Gly 290
295 300 Val Asn 305
45316PRTB.
thermoproteolyticusmisc_feature(1)..(316)B_thermoproteolyticus_P00800
45Ile Thr Gly Thr Ser Thr Val Gly Val Gly Arg Gly Val Leu Gly Asp 1
5 10 15 Gln Lys Asn Ile
Asn Thr Thr Tyr Ser Thr Tyr Tyr Tyr Leu Gln Asp 20
25 30 Asn Thr Arg Gly Asn Gly Ile Phe Thr
Tyr Asp Ala Lys Tyr Arg Thr 35 40
45 Thr Leu Pro Gly Ser Leu Trp Ala Asp Ala Asp Asn Gln Phe
Phe Ala 50 55 60
Ser Tyr Asp Ala Pro Ala Val Asp Ala His Tyr Tyr Ala Gly Val Thr 65
70 75 80 Tyr Asp Tyr Tyr Lys
Asn Val His Asn Arg Leu Ser Tyr Asp Gly Asn 85
90 95 Asn Ala Ala Ile Arg Ser Ser Val His Tyr
Ser Gln Gly Tyr Asn Asn 100 105
110 Ala Phe Trp Asn Gly Ser Gln Met Val Tyr Gly Asp Gly Asp Gly
Gln 115 120 125 Thr
Phe Ile Pro Leu Ser Gly Gly Ile Asp Val Val Ala His Glu Leu 130
135 140 Thr His Ala Val Thr Asp
Tyr Thr Ala Gly Leu Ile Tyr Gln Asn Glu 145 150
155 160 Ser Gly Ala Ile Asn Glu Ala Ile Ser Asp Ile
Phe Gly Thr Leu Val 165 170
175 Glu Phe Tyr Ala Asn Lys Asn Pro Asp Trp Glu Ile Gly Glu Asp Val
180 185 190 Tyr Thr
Pro Gly Ile Ser Gly Asp Ser Leu Arg Ser Met Ser Asp Pro 195
200 205 Ala Lys Tyr Gly Asp Pro Asp
His Tyr Ser Lys Arg Tyr Thr Gly Thr 210 215
220 Gln Asp Asn Gly Gly Val His Ile Asn Ser Gly Ile
Ile Asn Lys Ala 225 230 235
240 Ala Tyr Leu Ile Ser Gln Gly Gly Thr His Tyr Gly Val Ser Val Val
245 250 255 Gly Ile Gly
Arg Asp Lys Leu Gly Lys Ile Phe Tyr Arg Ala Leu Thr 260
265 270 Gln Tyr Leu Thr Pro Thr Ser Asn
Phe Ser Gln Leu Arg Ala Ala Ala 275 280
285 Val Gln Ser Ala Thr Asp Leu Tyr Gly Ser Thr Ser Gln
Glu Val Ala 290 295 300
Ser Val Lys Gln Ala Phe Asp Ala Val Gly Val Lys 305 310
315 46306PRTPaenibacillus sp.
Aloe-11misc_feature(1)..(306)ZP_09775365.1_P_sp_Aloe-11 46Ala Thr Gly Thr
Gly Arg Gly Val Asp Gly Lys Thr Lys Ser Phe Thr 1 5
10 15 Thr Thr Ala Ser Gly Asn Arg Tyr Gln
Leu Lys Asp Thr Thr Arg Ser 20 25
30 Asn Gly Ile Val Thr Tyr Thr Ala Gly Asn Arg Gln Thr Thr
Pro Gly 35 40 45
Thr Ile Leu Thr Asp Thr Asp Asn Val Trp Glu Asp Pro Ala Ala Val 50
55 60 Asp Ala His Ala Tyr
Ala Ile Lys Thr Tyr Asp Tyr Tyr Lys Asn Lys 65 70
75 80 Phe Gly Arg Asp Ser Ile Asp Gly Arg Gly
Met Gln Ile Arg Ser Thr 85 90
95 Val His Tyr Gly Lys Lys Tyr Asn Asn Ala Phe Trp Asn Gly Ser
Gln 100 105 110 Met
Thr Tyr Gly Asp Gly Asp Gly Ser Thr Phe Thr Phe Phe Ser Gly 115
120 125 Asp Pro Asp Val Val Gly
His Glu Leu Thr His Gly Val Thr Glu Phe 130 135
140 Thr Ser Asn Leu Glu Tyr Tyr Gly Glu Ser Gly
Ala Leu Asn Glu Ala 145 150 155
160 Phe Ser Asp Ile Ile Gly Asn Asp Ile Asp Gly Thr Ser Trp Leu Leu
165 170 175 Gly Asp
Gly Ile Tyr Thr Pro Asn Ile Pro Gly Asp Ala Leu Arg Ser 180
185 190 Leu Ser Asp Pro Thr Arg Phe
Gly Gln Pro Asp His Tyr Ser Asn Phe 195 200
205 Tyr Pro Asp Pro Asn Asn Asp Asp Glu Gly Gly Val
His Thr Asn Ser 210 215 220
Gly Ile Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Thr Ser 225
230 235 240 His Gly Val
Thr Val Thr Gly Ile Gly Arg Glu Ala Ala Val Phe Ile 245
250 255 Tyr Tyr Asn Ala Phe Thr Asn Tyr
Leu Thr Ser Thr Ser Asn Phe Ser 260 265
270 Asn Ala Arg Ala Ala Val Ile Gln Ala Ala Lys Asp Phe
Tyr Gly Ala 275 280 285
Asp Ser Leu Ala Val Thr Ser Ala Ile Gln Ser Phe Asp Ala Val Gly 290
295 300 Ile Lys 305
47304PRTP. terraemisc_feature(1)..(304)P_terrae_HPL-003_YP_005073223.
47Ala Thr Gly Thr Gly Lys Gly Val Leu Gly Asp Thr Lys Ser Phe Asn 1
5 10 15 Thr Thr Gln Ser
Gly Ser Ser Tyr Gln Leu Lys Asp Thr Thr Arg Gly 20
25 30 Asn Gly Ile Val Thr Tyr Thr Ala Ser
Asn Arg Gln Thr Ile Pro Gly 35 40
45 Thr Leu Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro Ala
Gly Val 50 55 60
Asp Ala His Ala Tyr Ala Ala Lys Thr Tyr Asp Tyr Tyr Lys Asp Lys 65
70 75 80 Phe Gly Arg Asn Ser
Ile Asp Gly Arg Gly Leu Gln Leu Arg Ser Thr 85
90 95 Val His Tyr Gly Ser Arg Tyr Asn Asn Ala
Phe Trp Asn Gly Ser Gln 100 105
110 Met Thr Tyr Gly Asp Gly Asp Gly Thr Thr Phe Ile Ala Phe Ser
Gly 115 120 125 Asp
Pro Asp Val Val Gly His Glu Leu Thr His Gly Val Thr Glu Tyr 130
135 140 Thr Ser Asn Leu Asp Tyr
Tyr Gly Glu Ser Gly Ala Leu Asn Glu Ser 145 150
155 160 Phe Ser Asp Ile Ile Gly Asn Asp Ile Gln Arg
Lys Asn Trp Leu Val 165 170
175 Gly Asp Asp Ile Tyr Thr Pro Ser Ile Ala Gly Asp Ala Leu Arg Ser
180 185 190 Met Ser
Asn Pro Thr Leu Tyr Asp Gln Pro Asp His Tyr Ser Asn Leu 195
200 205 Tyr Lys Gly Ser Ser Asp Asn
Gly Gly Val His Thr Asn Ser Gly Ile 210 215
220 Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly
Thr Phe His Asn 225 230 235
240 Val Thr Val Ser Gly Ile Gly Arg Asp Ala Ala Val Gln Ile Tyr Tyr
245 250 255 Ser Ala Phe
Thr Asn Tyr Leu Thr Ser Thr Ser Asn Phe Ser Asn Thr 260
265 270 Arg Ala Ala Val Val Gln Ala Ala
Lys Asp Leu Tyr Gly Ala Asn Ser 275 280
285 Ala Gln Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala Val
Gly Val Asn 290 295 300
48301PRTPaenibacillus
elgiimisc_feature(1)..(301)Paenibacillus_elgii_B69_ZP_090 48Ala Thr Gly
Thr Gly Lys Gly Val Leu Gly Asp Thr Lys Ser Phe Thr 1 5
10 15 Thr Thr Gln Ser Gly Ser Ser Tyr
Gln Leu Lys Asp Thr Thr Arg Gly 20 25
30 Gln Gly Ile Val Thr Tyr Ser Ala Gly Asn Arg Thr Ser
Leu Pro Gly 35 40 45
Ser Leu Leu Thr Ser Thr Asn Asn Ile Trp Asn Asp Gly Ser Ala Val 50
55 60 Asp Ala His Ala
Tyr Thr Gly Lys Val Tyr Asp Tyr Tyr Lys Asn Lys 65 70
75 80 Phe Gly Arg Asn Ser Ile Asp Gly Asn
Gly Leu Gln Leu Lys Ser Thr 85 90
95 Val His Tyr Ser Thr Arg Tyr Asn Asn Ala Phe Trp Asn Gly
Val Gln 100 105 110
Met Val Tyr Gly Asp Gly Asp Gly Val Thr Phe Arg Ser Phe Pro Ala
115 120 125 Asp Pro Asp Val
Ile Gly His Glu Leu Thr His Gly Val Thr Glu Ser 130
135 140 Thr Ala Gly Leu Glu Tyr Tyr Gly
Glu Ser Gly Ala Leu Asn Glu Ser 145 150
155 160 Ile Ser Asp Ile Phe Gly Asn Ala Ile Glu Gly Lys
Asn Trp Leu Ile 165 170
175 Gly Asp Leu Ile Thr Leu Asn Ala Gly Ala Leu Arg Ser Met Glu Asn
180 185 190 Pro Lys Leu
Tyr Arg Gln Pro Asp Arg Tyr Gln Asp Arg Tyr Thr Gly 195
200 205 Pro Ser Asp Asn Gly Gly Val His
Thr Asn Ser Gly Ile Asn Asn Lys 210 215
220 Ala Phe His Leu Ile Ala Gln Gly Gly Thr His Tyr Gly
Val Thr Val 225 230 235
240 Asn Gly Ile Gly Arg Ser Ala Ala Glu Gln Ile Phe Tyr Asp Ala Leu
245 250 255 Thr His Tyr Leu
Thr Pro Thr Ser Asn Phe Ser Ala Ile Arg Ala Ala 260
265 270 Ala Ile Gln Ala Ala Thr Asp Ser Phe
Gly Ala Asn Ser Ser Gln Val 275 280
285 Asp Ala Val Lys Lys Ala Tyr Asn Ala Val Gly Val Asn
290 295 300 49306PRTPaenibacillus
polymyxa SC2misc_feature(1)..(306)P_polymyxa_SC2 49Asn Glu Ala Thr Gly
Thr Gly Lys Gly Val Leu Gly Asp Ser Lys Ser 1 5
10 15 Phe Thr Thr Thr Ala Ser Gly Ser Ser Tyr
Gln Leu Lys Asp Thr Thr 20 25
30 Arg Gly Asn Gly Ile Val Thr Tyr Thr Ala Ser Asn Arg Gln Ser
Ile 35 40 45 Pro
Gly Thr Ile Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro Ala 50
55 60 Gly Val Asp Ala His Ala
Tyr Ala Ala Lys Thr Tyr Asp Tyr Tyr Lys 65 70
75 80 Ala Lys Phe Gly Arg Asn Ser Ile Asp Gly Arg
Gly Leu Gln Leu Arg 85 90
95 Ser Thr Val His Tyr Gly Ser Arg Tyr Asn Asn Ala Phe Trp Asn Gly
100 105 110 Ser Gln
Met Thr Tyr Gly Asp Gly Asp Gly Ser Thr Phe Ile Ala Phe 115
120 125 Ser Gly Asp Pro Asp Val Val
Gly His Glu Leu Thr His Gly Val Thr 130 135
140 Glu Tyr Thr Ser Asn Leu Glu Tyr Tyr Gly Glu Ser
Gly Ala Leu Asn 145 150 155
160 Glu Ala Phe Ser Asp Val Ile Gly Asn Asp Ile Gln Arg Lys Asn Trp
165 170 175 Leu Val Gly
Asp Asp Ile Tyr Thr Pro Asn Ile Ala Gly Asp Ala Leu 180
185 190 Arg Ser Met Ser Asn Pro Thr Leu
Tyr Asp Gln Pro Asp His Tyr Ser 195 200
205 Asn Leu Tyr Arg Gly Ser Ser Asp Asn Gly Gly Val His
Thr Asn Ser 210 215 220
Gly Ile Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Asn Phe 225
230 235 240 His Gly Val Thr
Val Asn Gly Ile Gly Arg Asp Ala Ala Val Gln Ile 245
250 255 Tyr Tyr Ser Ala Phe Thr Asn Tyr Leu
Thr Ser Ser Ser Asp Phe Ser 260 265
270 Asn Ala Arg Ala Ala Val Ile Gln Ala Ala Lys Asp Leu Tyr
Gly Ala 275 280 285
Asn Ser Ala Glu Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala Val Gly 290
295 300 Val Asn 305
50306PRTPaenibacillus polymyxa
SC2misc_feature(1)..(306)P_polymyxa_SC2_YP_003948511.1 50Asn Glu Ala Thr
Gly Thr Gly Lys Gly Val Leu Gly Asp Ser Lys Ser 1 5
10 15 Phe Thr Thr Thr Ala Ser Gly Ser Ser
Tyr Gln Leu Lys Asp Thr Thr 20 25
30 Arg Gly Asn Gly Ile Val Thr Tyr Thr Ala Ser Asn Arg Gln
Ser Ile 35 40 45
Pro Gly Thr Ile Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro Ala 50
55 60 Gly Val Asp Ala His
Ala Tyr Ala Ala Lys Thr Tyr Asp Tyr Tyr Lys 65 70
75 80 Ala Lys Phe Gly Arg Asn Ser Ile Asp Gly
Arg Gly Leu Gln Leu Arg 85 90
95 Ser Thr Val His Tyr Gly Ser Arg Tyr Asn Asn Ala Phe Trp Asn
Gly 100 105 110 Ser
Gln Met Thr Tyr Gly Asp Gly Asp Gly Ser Thr Phe Ile Ala Phe 115
120 125 Ser Gly Asp Pro Asp Val
Val Gly His Glu Leu Thr His Gly Val Thr 130 135
140 Glu Tyr Thr Ser Asn Leu Glu Tyr Tyr Gly Glu
Ser Gly Ala Leu Asn 145 150 155
160 Glu Ala Phe Ser Asp Val Ile Gly Asn Asp Ile Gln Arg Lys Asn Trp
165 170 175 Leu Val
Gly Asp Asp Ile Tyr Thr Pro Asn Ile Ala Gly Asp Ala Leu 180
185 190 Arg Ser Met Ser Asn Pro Thr
Leu Tyr Asp Gln Pro Asp His Tyr Ser 195 200
205 Asn Leu Tyr Arg Gly Ser Ser Asp Asn Gly Gly Val
His Thr Asn Ser 210 215 220
Gly Ile Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Asn Phe 225
230 235 240 His Gly Val
Thr Val Asn Gly Ile Gly Arg Asp Ala Ala Val Gln Ile 245
250 255 Tyr Tyr Ser Ala Phe Thr Asn Tyr
Leu Thr Ser Ser Ser Asp Phe Ser 260 265
270 Asn Ala Arg Ala Ala Val Ile Gln Ala Ala Lys Asp Leu
Tyr Gly Ala 275 280 285
Asn Ser Ala Glu Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala Val Gly 290
295 300 Val Asn 305
51304PRTP. terraemisc_feature(1)..(304)P_terrae_HPL-003_YP_005073223
51Ala Thr Gly Thr Gly Lys Gly Val Leu Gly Asp Thr Lys Ser Phe Asn 1
5 10 15 Thr Thr Gln Ser
Gly Ser Ser Tyr Gln Leu Lys Asp Thr Thr Arg Gly 20
25 30 Asn Gly Ile Val Thr Tyr Thr Ala Ser
Asn Arg Gln Thr Ile Pro Gly 35 40
45 Thr Leu Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro Ala
Gly Val 50 55 60
Asp Ala His Ala Tyr Ala Ala Lys Thr Tyr Asp Tyr Tyr Lys Asp Lys 65
70 75 80 Phe Gly Arg Asn Ser
Ile Asp Gly Arg Gly Leu Gln Leu Arg Ser Thr 85
90 95 Val His Tyr Gly Ser Arg Tyr Asn Asn Ala
Phe Trp Asn Gly Ser Gln 100 105
110 Met Thr Tyr Gly Asp Gly Asp Gly Thr Thr Phe Ile Ala Phe Ser
Gly 115 120 125 Asp
Pro Asp Val Val Gly His Glu Leu Thr His Gly Val Thr Glu Tyr 130
135 140 Thr Ser Asn Leu Asp Tyr
Tyr Gly Glu Ser Gly Ala Leu Asn Glu Ser 145 150
155 160 Phe Ser Asp Ile Ile Gly Asn Asp Ile Gln Arg
Lys Asn Trp Leu Val 165 170
175 Gly Asp Asp Ile Tyr Thr Pro Ser Ile Ala Gly Asp Ala Leu Arg Ser
180 185 190 Met Ser
Asn Pro Thr Leu Tyr Asp Gln Pro Asp His Tyr Ser Asn Leu 195
200 205 Tyr Lys Gly Ser Ser Asp Asn
Gly Gly Val His Thr Asn Ser Gly Ile 210 215
220 Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly
Thr Phe His Asn 225 230 235
240 Val Thr Val Ser Gly Ile Gly Arg Asp Ala Ala Val Gln Ile Tyr Tyr
245 250 255 Ser Ala Phe
Thr Asn Tyr Leu Thr Ser Thr Ser Asn Phe Ser Asn Thr 260
265 270 Arg Ala Ala Val Val Gln Ala Ala
Lys Asp Leu Tyr Gly Ala Asn Ser 275 280
285 Ala Gln Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala Val
Gly Val Asn 290 295 300
52309PRTP. peoriaemisc_feature(1)..(309)P_peoriae_KCTC 52Asp Ile Ile
Asn Glu Ala Thr Gly Thr Gly Lys Gly Val Leu Gly Asp 1 5
10 15 Thr Lys Ser Phe Thr Thr Thr Ala
Ser Gly Ser Ser Tyr Gln Leu Arg 20 25
30 Asp Thr Thr Arg Gly Asn Gly Ile Val Thr Tyr Thr Ala
Ser Asn Arg 35 40 45
Gln Ser Ile Pro Gly Thr Ile Leu Thr Asp Ala Asp Asn Val Trp Asn 50
55 60 Asp Pro Ala Gly
Val Asp Ala His Ala Tyr Ala Ala Lys Thr Tyr Asp 65 70
75 80 Tyr Tyr Lys Glu Lys Phe Asn Arg Asn
Ser Ile Asp Gly Arg Gly Leu 85 90
95 Gln Leu Arg Ser Thr Val His Tyr Gly Asn Arg Tyr Asn Asn
Ala Phe 100 105 110
Trp Asn Gly Ser Gln Met Thr Tyr Gly Asp Gly Asp Gly Thr Thr Phe
115 120 125 Ile Ala Phe Ser
Gly Asp Pro Asp Val Val Gly His Glu Leu Thr His 130
135 140 Gly Val Thr Glu Tyr Thr Ser Asn
Leu Glu Tyr Tyr Gly Glu Ser Gly 145 150
155 160 Ala Leu Asn Glu Ser Phe Ser Asp Ile Ile Gly Asn
Asp Ile Gln Arg 165 170
175 Lys Asn Trp Leu Val Gly Asp Asp Ile Tyr Thr Pro Arg Ile Ala Gly
180 185 190 Asp Ala Leu
Arg Ser Met Ser Asn Pro Thr Leu Tyr Asp Gln Pro Asp 195
200 205 His Tyr Ser Asn Leu Tyr Arg Gly
Ser Ser Asp Asn Gly Gly Val His 210 215
220 Thr Asn Ser Gly Ile Ile Asn Lys Ala Tyr Tyr Leu Leu
Ala Gln Gly 225 230 235
240 Gly Thr Phe His Gly Val Thr Val Asn Gly Ile Gly Arg Asp Ala Ala
245 250 255 Val Gln Ile Tyr
Tyr Ser Ala Phe Thr Asn Tyr Leu Thr Ser Ser Ser 260
265 270 Asp Phe Ser Asn Ala Arg Asp Ala Val
Val Gln Ala Ala Lys Asp Leu 275 280
285 Tyr Gly Ala Ser Ser Ala Gln Ala Thr Ala Ala Ala Lys Ala
Phe Asp 290 295 300
Ala Val Gly Val Asn 305 53316PRTBacillus
thermoproteolyticusmisc_feature(1)..(316)1KEI.A 53Ile Thr Gly Thr Ser Thr
Val Gly Val Gly Arg Gly Val Leu Gly Asp 1 5
10 15 Gln Lys Asn Ile Asn Thr Thr Tyr Ser Thr Tyr
Tyr Tyr Leu Gln Asp 20 25
30 Asn Thr Arg Gly Asn Gly Ile Phe Thr Tyr Asp Ala Lys Tyr Arg
Thr 35 40 45 Thr
Leu Pro Gly Ser Leu Trp Ala Asp Ala Asp Asn Gln Phe Phe Ala 50
55 60 Ser Tyr Asp Ala Pro Ala
Val Asp Ala His Tyr Tyr Ala Gly Val Thr 65 70
75 80 Tyr Asp Tyr Tyr Lys Asn Val His Asn Arg Leu
Ser Tyr Asp Gly Asn 85 90
95 Asn Ala Ala Ile Arg Ser Ser Val His Tyr Ser Gln Gly Tyr Asn Asn
100 105 110 Ala Phe
Trp Asn Gly Ser Gln Met Val Tyr Gly Asp Gly Asp Gly Gln 115
120 125 Thr Phe Ile Pro Leu Ser Gly
Gly Ile Asp Val Val Ala His Glu Leu 130 135
140 Thr His Ala Val Thr Asp Tyr Thr Ala Gly Leu Ile
Tyr Gln Asn Glu 145 150 155
160 Ser Gly Ala Ile Asn Glu Ala Ile Ser Asp Ile Phe Gly Thr Leu Val
165 170 175 Glu Phe Tyr
Ala Asn Lys Asn Pro Asp Trp Glu Ile Gly Glu Asp Val 180
185 190 Tyr Thr Pro Gly Ile Ser Gly Asp
Ser Leu Arg Ser Met Ser Asp Pro 195 200
205 Ala Lys Tyr Gly Asp Pro Asp His Tyr Ser Lys Arg Tyr
Thr Gly Thr 210 215 220
Gln Asp Asn Gly Gly Val His Ile Asn Ser Gly Ile Ile Asn Lys Ala 225
230 235 240 Ala Tyr Leu Ile
Ser Gln Gly Gly Thr His Tyr Gly Val Ser Val Val 245
250 255 Gly Ile Gly Arg Asp Lys Leu Gly Lys
Ile Phe Tyr Arg Ala Leu Thr 260 265
270 Gln Tyr Leu Thr Pro Thr Ser Asn Phe Ser Gln Leu Arg Ala
Ala Ala 275 280 285
Val Gln Ser Ala Thr Asp Leu Tyr Gly Ser Thr Ser Gln Glu Val Ala 290
295 300 Ser Val Lys Gln Ala
Phe Asp Ala Val Gly Val Lys 305 310 315
54316PRTB. caldolyticusmisc_feature(1)..(316)B_caldolyticus_AAA22623.1
54Thr Ser Thr Val Gly Val Gly Arg Gly Val Leu Gly Asp Gln Lys Tyr 1
5 10 15 Ile Asn Thr Thr
Tyr Ser Ser Tyr Tyr Gly Tyr Tyr Tyr Leu Gln Asp 20
25 30 Asn Thr Arg Gly Ser Gly Ile Phe Thr
Tyr Asp Gly Arg Asn Arg Thr 35 40
45 Val Leu Pro Gly Ser Leu Trp Ala Asp Gly Asp Asn Gln Phe
Phe Ala 50 55 60
Ser Tyr Asp Ala Ala Ala Val Asp Ala His Tyr Tyr Ala Gly Val Val 65
70 75 80 Tyr Asp Tyr Tyr Lys
Asn Val His Gly Arg Leu Ser Tyr Asp Gly Ser 85
90 95 Asn Ala Ala Ile Arg Ser Thr Val His Tyr
Gly Arg Gly Tyr Asn Asn 100 105
110 Ala Phe Trp Asn Gly Ser Gln Met Val Tyr Gly Asp Gly Asp Gly
Gln 115 120 125 Thr
Phe Leu Pro Phe Ser Gly Gly Ile Asp Val Val Gly His Glu Leu 130
135 140 Thr His Ala Val Thr Asp
Tyr Thr Ala Gly Leu Val Tyr Gln Asn Glu 145 150
155 160 Ser Gly Ala Ile Asn Glu Ala Met Ser Asp Ile
Phe Gly Thr Leu Val 165 170
175 Glu Phe Tyr Ala Asn Arg Asn Pro Asp Trp Glu Ile Gly Glu Asp Ile
180 185 190 Tyr Thr
Pro Gly Val Ala Gly Asp Ala Leu Arg Ser Met Ser Asp Pro 195
200 205 Ala Lys Tyr Gly Asp Pro Asp
His Tyr Ser Lys Arg Tyr Thr Gly Thr 210 215
220 Gln Asp Asn Gly Gly Val His Thr Asn Ser Gly Ile
Ile Asn Lys Ala 225 230 235
240 Ala Tyr Leu Leu Ser Gln Gly Gly Val His Tyr Gly Val Ser Val Thr
245 250 255 Gly Ile Gly
Arg Asp Lys Met Gly Lys Ile Phe Tyr Arg Ala Leu Val 260
265 270 Tyr Tyr Leu Thr Pro Thr Ser Asn
Phe Ser Gln Leu Arg Ala Ala Cys 275 280
285 Val Gln Ala Ala Ala Asp Leu Tyr Gly Ser Thr Ser Gln
Glu Val Asn 290 295 300
Ser Val Lys Gln Ala Phe Asn Ala Val Gly Val Tyr 305 310
315 55292PRTB.
anthracismisc_feature(1)..(292)B_anthracis_NP843132.1 55Val Thr Gly Thr
Asn Ala Val Gly Thr Gly Lys Gly Val Leu Gly Asp 1 5
10 15 Thr Lys Ser Leu Asn Thr Thr Leu Ser
Ala Ser Ser Tyr Tyr Leu Gln 20 25
30 Asp Asn Thr Arg Gly Ala Thr Ile Phe Thr Tyr Asp Ala Lys
Asn Arg 35 40 45
Ser Thr Leu Pro Gly Thr Leu Trp Val Asp Ala Asp Asn Val Phe Asn 50
55 60 Ala Ala Tyr Asp Ala
Ala Ala Val Asp Ala His Tyr Tyr Ala Gly Arg 65 70
75 80 Thr Tyr Asp Tyr Tyr Lys Ala Thr Phe Asn
Arg Asn Ser Ile Asn Asp 85 90
95 Ala Gly Ala Pro Leu Lys Ser Thr Val His Tyr Gly Ser Arg Tyr
Asn 100 105 110 Asn
Ala Phe Trp Asn Gly Ser Gln Met Val Tyr Gly Asp Gly Asp Gly 115
120 125 Val Thr Phe Thr Ser Leu
Ser Gly Gly Ile Asp Val Ile Gly His Glu 130 135
140 Leu Thr His Ala Val Thr Glu Tyr Ser Ser Asp
Leu Ile Tyr Gln Asn 145 150 155
160 Glu Ser Gly Ala Leu Asn Glu Ala Ile Ser Asp Val Phe Gly Thr Leu
165 170 175 Val Glu
Tyr Tyr Asp Asn Arg Asn Pro Asp Trp Glu Ile Gly Glu Asp 180
185 190 Ile Tyr Thr Pro Gly Lys Ala
Gly Asp Ala Leu Arg Ser Met Ser Asp 195 200
205 Pro Thr Lys Tyr Gly Asp Pro Asp His Tyr Ser Lys
Arg Tyr Thr Gly 210 215 220
Thr Gly Asp Asn Gly Gly Val His Thr Asn Ser Gly Ile Ile Asn Lys 225
230 235 240 Ala Ala Tyr
Leu Leu Ala Asn Gly Gly Thr His Tyr Gly Val Thr Val 245
250 255 Asn Gly Ile Gly Lys Asp Lys Val
Gly Ala Ile Tyr Tyr Arg Ala Asn 260 265
270 Thr Gln Tyr Phe Thr Gln Ser Thr Thr Phe Ser Gln Ala
Arg Ala Gly 275 280 285
Leu Val Gln Ala 290 56317PRTB.
thuringiensismisc_feature(1)..(317)B_thuringiensis_YP893436.1 56Val Thr
Gly Thr Asn Ala Val Gly Thr Gly Lys Gly Val Leu Gly Asp 1 5
10 15 Thr Lys Ser Leu Asn Thr Thr
Leu Ser Ala Ser Ser Tyr Tyr Leu Gln 20 25
30 Asp Asn Thr Arg Gly Ala Thr Ile Phe Thr Tyr Asp
Ala Lys Asn Arg 35 40 45
Ser Thr Leu Pro Gly Thr Leu Trp Val Asp Ala Asp Asn Val Phe Asn
50 55 60 Ala Ala Tyr
Asp Ala Ala Ala Val Asp Ala His Tyr Tyr Ala Gly Lys 65
70 75 80 Thr Tyr Asp Tyr Tyr Lys Ala
Thr Phe Asn Arg Asn Ser Ile Asn Asp 85
90 95 Ala Gly Ala Pro Leu Lys Ser Thr Val His Tyr
Gly Ser Arg Tyr Asn 100 105
110 Asn Ala Phe Trp Asn Gly Ser Gln Met Val Tyr Gly Asp Gly Asp
Gly 115 120 125 Val
Thr Phe Thr Ser Leu Ser Gly Gly Ile Asp Val Ile Gly His Glu 130
135 140 Leu Thr His Ala Val Thr
Glu Tyr Ser Ser Asp Leu Ile Tyr Gln Asn 145 150
155 160 Glu Ser Gly Ala Leu Asn Glu Ala Ile Ser Asp
Val Phe Gly Thr Leu 165 170
175 Val Glu Phe Tyr Asp Asn Arg Asn Pro Asp Trp Glu Ile Gly Glu Asp
180 185 190 Ile Tyr
Thr Pro Gly Lys Ala Gly Asp Ala Leu Arg Ser Met Ser Asp 195
200 205 Pro Thr Lys Tyr Gly Asp Pro
Asp His Tyr Ser Lys Arg Tyr Thr Gly 210 215
220 Thr Gly Asp Asn Gly Gly Val His Thr Asn Ser Gly
Ile Ile Asn Lys 225 230 235
240 Ala Ala Tyr Leu Leu Ala Asn Gly Gly Thr His Tyr Gly Val Thr Val
245 250 255 Asn Gly Ile
Gly Lys Asp Lys Val Gly Ala Ile Tyr Tyr Arg Ala Asn 260
265 270 Thr Gln Tyr Phe Thr Gln Ser Thr
Thr Phe Ser Gln Ala Arg Ala Gly 275 280
285 Leu Val Gln Ala Ala Thr Asp Leu Tyr Gly Ala Ser Ser
Ala Glu Val 290 295 300
Ala Ala Val Lys Gln Ser Tyr Ser Ala Val Gly Val Asn 305
310 315 57314PRTB.
cereusmisc_feature(1)..(314)B_cereus_ZP04310163.1 57Thr Asn Ala Val Gly
Thr Gly Lys Gly Val Leu Gly Asp Thr Lys Ser 1 5
10 15 Leu Asn Thr Thr Leu Ser Ala Ser Ser Tyr
Tyr Leu Gln Asp Asn Thr 20 25
30 Arg Gly Ala Thr Ile Phe Thr Tyr Asp Ala Lys Asn Arg Ser Thr
Leu 35 40 45 Pro
Gly Thr Leu Trp Val Asp Ala Asp Asn Val Phe Asn Ala Ala Tyr 50
55 60 Asp Ala Ala Ala Val Asp
Ala His Tyr Tyr Ala Gly Lys Thr Tyr Asp 65 70
75 80 Tyr Tyr Lys Ala Thr Phe Asn Arg Asn Ser Ile
Asn Asp Ala Gly Ala 85 90
95 Pro Leu Lys Ser Thr Val His Tyr Gly Ser Arg Tyr Asn Asn Ala Phe
100 105 110 Trp Asn
Gly Ser Gln Met Val Tyr Gly Asp Gly Asp Gly Val Thr Phe 115
120 125 Thr Ser Leu Ser Gly Gly Ile
Asp Val Ile Gly His Glu Leu Thr His 130 135
140 Ala Val Thr Glu Tyr Ser Ser Asp Leu Ile Tyr Gln
Asn Glu Ser Gly 145 150 155
160 Ala Leu Asn Glu Ala Ile Ser Asp Val Phe Gly Thr Leu Val Glu Phe
165 170 175 Tyr Asp Asn
Arg Asn Pro Asp Trp Glu Ile Gly Glu Asp Ile Tyr Thr 180
185 190 Pro Gly Lys Ala Gly Asp Ala Leu
Arg Ser Met Ser Asp Pro Thr Lys 195 200
205 Tyr Gly Asp Pro Asp His Tyr Ser Lys Arg Tyr Thr Gly
Thr Gly Asp 210 215 220
Asn Gly Gly Val His Thr Asn Ser Gly Ile Ile Asn Lys Ala Ala Tyr 225
230 235 240 Leu Leu Ala Asn
Gly Gly Thr His Tyr Gly Val Thr Val Asn Gly Ile 245
250 255 Gly Lys Asp Lys Val Gly Ala Ile Tyr
Tyr Arg Ala Asn Thr Gln Tyr 260 265
270 Phe Thr Gln Ser Thr Thr Phe Ser Gln Ala Arg Ala Gly Leu
Val Gln 275 280 285
Ala Ala Ala Asp Leu Tyr Gly Ala Ser Ser Ala Glu Val Ala Ala Val 290
295 300 Lys Gln Ser Tyr Ser
Ala Val Gly Val Asn 305 310
58317PRTLactobacillus
sp.misc_feature(1)..(317)Lactobacillus_sp_BAA06144.1 58Val Thr Gly Thr
Asn Ala Val Gly Thr Gly Lys Gly Val Leu Gly Asp 1 5
10 15 Thr Lys Ser Leu Asn Thr Thr Leu Ser
Ala Ser Ser Tyr Tyr Leu Gln 20 25
30 Asp Asn Thr Arg Gly Ala Thr Ile Phe Thr Tyr Asp Ala Lys
Asn Arg 35 40 45
Ser Thr Leu Pro Gly Thr Leu Trp Val Asp Ala Asp Asn Val Phe Asn 50
55 60 Ala Ala Tyr Asp Ala
Ala Ala Val Asp Ala His Tyr Tyr Ala Gly Lys 65 70
75 80 Thr Tyr Asp Tyr Tyr Lys Ala Thr Phe Asn
Arg Asn Ser Ile Asn Asp 85 90
95 Ala Gly Ala Pro Leu Lys Ser Thr Val His Tyr Gly Ser Lys Tyr
Asn 100 105 110 Asn
Ala Phe Trp Asn Gly Ser Gln Met Val Tyr Gly Asp Gly Asp Gly 115
120 125 Val Thr Phe Thr Ser Leu
Ser Gly Gly Ile Asp Val Ile Gly His Glu 130 135
140 Leu Thr His Ala Val Thr Glu Tyr Ser Ser Asp
Leu Ile Tyr Gln Asn 145 150 155
160 Glu Ser Gly Ala Leu Asn Glu Ala Ile Ser Asp Val Phe Gly Thr Leu
165 170 175 Val Glu
Tyr Tyr Asp Asn Arg Asn Pro Asp Trp Glu Ile Gly Glu Asp 180
185 190 Ile Tyr Thr Pro Gly Lys Ala
Gly Asp Ala Leu Arg Ser Met Ser Asp 195 200
205 Pro Thr Lys Tyr Gly Asp Pro Asp His Tyr Ser Lys
Arg Tyr Thr Gly 210 215 220
Thr Ser Asp Asn Gly Gly Val His Thr Asn Ser Gly Ile Ile Asn Lys 225
230 235 240 Ala Ala Tyr
Leu Leu Ala Asn Gly Gly Thr His Tyr Gly Val Thr Val 245
250 255 Asn Gly Ile Gly Lys Asp Lys Val
Gly Ala Ile Tyr Tyr Arg Ala Asn 260 265
270 Thr Gln Tyr Phe Thr Gln Ser Thr Thr Phe Ser Gln Ala
Arg Ala Gly 275 280 285
Leu Val Gln Ala Ala Ala Asp Leu Tyr Gly Ala Ser Ser Ala Glu Val 290
295 300 Ala Ala Val Lys
Gln Ser Tyr Ser Ala Val Gly Val Asn 305 310
315 59317PRTBacillus
thermoproteolyticusmisc_feature(1)..(317)1NPC.A 59Val Thr Gly Thr Asn Lys
Val Gly Thr Gly Lys Gly Val Leu Gly Asp 1 5
10 15 Thr Lys Ser Leu Asn Thr Thr Leu Ser Gly Ser
Ser Tyr Tyr Leu Gln 20 25
30 Asp Asn Thr Arg Gly Ala Thr Ile Phe Thr Tyr Asp Ala Lys Asn
Arg 35 40 45 Ser
Thr Leu Pro Gly Thr Leu Trp Ala Asp Ala Asp Asn Val Phe Asn 50
55 60 Ala Ala Tyr Asp Ala Ala
Ala Val Asp Ala His Tyr Tyr Ala Gly Lys 65 70
75 80 Thr Tyr Asp Tyr Tyr Lys Ala Thr Phe Asn Arg
Asn Ser Ile Asn Asp 85 90
95 Ala Gly Ala Pro Leu Lys Ser Thr Val His Tyr Gly Ser Asn Tyr Asn
100 105 110 Asn Ala
Phe Trp Asn Gly Ser Gln Met Val Tyr Gly Asp Gly Asp Gly 115
120 125 Val Thr Phe Thr Ser Leu Ser
Gly Gly Ile Asp Val Ile Gly His Glu 130 135
140 Leu Thr His Ala Val Thr Glu Asn Ser Ser Asn Leu
Ile Tyr Gln Asn 145 150 155
160 Glu Ser Gly Ala Leu Asn Glu Ala Ile Ser Asp Ile Phe Gly Thr Leu
165 170 175 Val Glu Phe
Tyr Asp Asn Arg Asn Pro Asp Trp Glu Ile Gly Glu Asp 180
185 190 Ile Tyr Thr Pro Gly Lys Ala Gly
Asp Ala Leu Arg Ser Met Ser Asp 195 200
205 Pro Thr Lys Tyr Gly Asp Pro Asp His Tyr Ser Lys Arg
Tyr Thr Gly 210 215 220
Ser Ser Asp Asn Gly Gly Val His Thr Asn Ser Gly Ile Ile Asn Lys 225
230 235 240 Gln Ala Tyr Leu
Leu Ala Asn Gly Gly Thr His Tyr Gly Val Thr Val 245
250 255 Thr Gly Ile Gly Lys Asp Lys Leu Gly
Ala Ile Tyr Tyr Arg Ala Asn 260 265
270 Thr Gln Tyr Phe Thr Gln Ser Thr Thr Phe Ser Gln Ala Arg
Ala Gly 275 280 285
Ala Val Gln Ala Ala Ala Asp Leu Tyr Gly Ala Asn Ser Ala Glu Val 290
295 300 Ala Ala Val Lys Gln
Ser Phe Ser Ala Val Gly Val Asn 305 310
315 60317PRTB.
cytotoxicusmisc_feature(1)..(317)B_cytotoxicus_YP001373863.1 60Val Thr
Gly Thr Asn Ala Val Gly Thr Gly Thr Gly Val Leu Gly Asp 1 5
10 15 Lys Lys Ser Ile Asn Thr Thr
Leu Ser Gly Ser Thr Tyr Tyr Leu Gln 20 25
30 Asp Asn Thr Arg Gly Ala Gln Ile Phe Thr Tyr Asp
Ala Lys Asn Arg 35 40 45
Ser Ser Leu Pro Gly Thr Leu Trp Ala Asp Val Asp Asn Ala Phe His
50 55 60 Ala Lys Tyr
Asp Ala Ala Ala Val Asp Ala His Tyr Tyr Ala Gly Val 65
70 75 80 Thr Tyr Asp Tyr Tyr Lys Asn
Thr Phe Asn Arg Asn Ser Ile Asn Asp 85
90 95 Ala Gly Ala Ala Leu Lys Ser Thr Val His Tyr
Gly Ser Asn Tyr Asn 100 105
110 Asn Ala Phe Trp Asn Gly Ser Gln Met Val Tyr Gly Asp Gly Asp
Gly 115 120 125 Val
Thr Phe Thr Ser Leu Ser Gly Gly Ile Asp Val Ile Gly His Glu 130
135 140 Leu Thr His Ala Val Thr
Glu Tyr Ser Ser Asn Leu Ile Tyr Gln Tyr 145 150
155 160 Glu Ser Gly Ala Leu Asn Glu Ala Ile Ser Asp
Ile Phe Gly Thr Leu 165 170
175 Val Glu Tyr Tyr Asp Asn Arg Asn Pro Asp Trp Glu Ile Gly Glu Asp
180 185 190 Ile Tyr
Thr Pro Gly Lys Ala Gly Asp Ala Leu Arg Ser Met Ser Asp 195
200 205 Pro Thr Lys Tyr Gly Asp Pro
Asp His Tyr Ser Lys Arg Tyr Thr Gly 210 215
220 Ser Gly Asp Asn Gly Gly Val His Thr Asn Ser Gly
Ile Ile Asn Lys 225 230 235
240 Ala Ala Tyr Leu Leu Ala Asn Gly Gly Thr His Tyr Gly Val Thr Val
245 250 255 Asn Gly Ile
Gly Lys Asp Lys Val Gly Ala Ile Tyr Tyr Arg Ala Asn 260
265 270 Thr Gln Tyr Phe Thr Gln Ser Thr
Thr Phe Ser Gln Ala Arg Ala Gly 275 280
285 Leu Val Gln Ala Ala Ala Asp Leu Tyr Gly Ala Asn Ser
Ala Glu Val 290 295 300
Thr Ala Val Lys Gln Ser Tyr Asp Ala Val Gly Val Lys 305
310 315 61314PRTB.
megateriummisc_feature(1)..(314)B_megaterium_YP005495105.1 61Thr Asn Ala
Ile Gly Ser Gly Lys Gly Val Leu Gly Asp Thr Lys Ser 1 5
10 15 Leu Lys Thr Thr Leu Ser Gly Ser
Ala Tyr Tyr Leu Gln Asp Asn Thr 20 25
30 Arg Gly Ala Thr Ile Tyr Thr Tyr Asp Ala Lys Asn Arg
Thr Ser Leu 35 40 45
Pro Gly Thr Leu Trp Ala Asp Thr Asp Asn Thr Tyr Asn Ala Thr Arg 50
55 60 Asp Ala Ala Ala
Val Asp Ala His Tyr Tyr Ala Gly Val Thr Tyr Asp 65 70
75 80 Tyr Tyr Lys Asn Lys Phe Asn Arg Asn
Ser Tyr Asp Asn Ala Gly Ala 85 90
95 Pro Leu Lys Ser Thr Val His Tyr Ser Ser Gly Tyr Asn Asn
Ala Phe 100 105 110
Trp Asn Gly Ser Gln Met Val Tyr Gly Asp Gly Asp Gly Thr Thr Phe
115 120 125 Val Pro Leu Ser
Gly Gly Leu Asp Val Ile Gly His Glu Leu Thr His 130
135 140 Ala Val Thr Glu Arg Ser Ser Asn
Leu Ile Tyr Gln Tyr Glu Ser Gly 145 150
155 160 Ala Leu Asn Glu Ala Ile Ser Asp Ile Phe Gly Thr
Leu Val Glu Tyr 165 170
175 Tyr Asp Asn Arg Asn Pro Asp Trp Glu Ile Gly Glu Asp Ile Tyr Thr
180 185 190 Pro Gly Thr
Ser Gly Asp Ala Leu Arg Ser Met Ser Asn Pro Ala Lys 195
200 205 Tyr Gly Asp Pro Asp His Tyr Ser
Lys Arg Tyr Thr Gly Ser Ser Asp 210 215
220 Asn Gly Gly Val His Thr Asn Ser Gly Ile Ile Asn Lys
Ala Ala Tyr 225 230 235
240 Leu Leu Ala Asn Gly Gly Thr His Tyr Gly Val Thr Val Thr Gly Ile
245 250 255 Gly Gly Asp Lys
Leu Gly Lys Ile Tyr Tyr Arg Ala Asn Thr Leu Tyr 260
265 270 Phe Thr Gln Ser Thr Thr Phe Ser Gln
Ala Arg Ala Gly Leu Val Gln 275 280
285 Ala Ala Ala Asp Leu Tyr Gly Ser Gly Ser Gln Glu Val Ile
Ser Val 290 295 300
Gly Lys Ser Phe Asp Ala Val Gly Val Gln 305 310
62322PRTBacillus sp.
SG-1misc_feature(1)..(322)B_sp_SG-1_ZP01858398.1 62Val Ser Gly Thr Asp
Gln Val Gly Thr Gly Lys Gly Val Leu Gly Asp 1 5
10 15 Thr Lys Ser Leu Asn Thr Thr Leu Ser Asn
Gly Thr Tyr Tyr Leu Gln 20 25
30 Asp Asn Thr Arg Gly Gly Gly Ile Met Thr Tyr Asp Met Lys Asn
Arg 35 40 45 Thr
Phe Phe Pro Gln Phe Tyr Leu Pro Gly Ser Leu Trp Ser Asp Ala 50
55 60 Asp Asn Val Tyr Asn Gln
Ala Tyr Asp Ala Ala Ala Val Asp Ala His 65 70
75 80 Tyr Phe Ala Gly Ala Thr Phe Asp Tyr Tyr Lys
Asp Val Phe Gly Arg 85 90
95 Asn Ser Tyr Asp Asn Lys Gly Thr Thr Ile Gln Ser Ser Val His Tyr
100 105 110 Ser Lys
Asn Tyr Asn Asn Ala Phe Trp Asn Gly Ser Gln Met Val Tyr 115
120 125 Gly Asp Gly Asp Gly Thr Thr
Phe Ile Pro Leu Ser Gly Gly Leu Asp 130 135
140 Val Val Ala His Glu Leu Thr His Ala Val Thr Asp
Thr Ser Ser Asp 145 150 155
160 Leu Val Tyr Gln Asn Glu Ser Gly Ala Leu Asn Glu Ala Ile Ser Asp
165 170 175 Ile Phe Gly
Thr Leu Val Glu Tyr His Glu Asn His Asn Pro Asp Phe 180
185 190 Glu Ile Gly Glu Asp Ile Tyr Thr
Pro Asn Thr Pro Asn Asp Ala Leu 195 200
205 Arg Ser Met Ser Asp Pro Ala Lys Tyr Gly Asp Pro Asp
His Tyr Ser 210 215 220
Val Arg Tyr Thr Gly Thr Gln Asp Asn Gly Gly Val His Ile Asn Ser 225
230 235 240 Gly Ile Ile Asn
Lys Gln Ala Tyr Leu Leu Ser Glu Gly Gly Thr His 245
250 255 Tyr Gly Val Asn Val Thr Gly Ile Gly
Arg Glu Lys Leu Gly Glu Ile 260 265
270 Tyr Tyr Arg Met Asn Thr Val Tyr Leu Thr Ala Ser Ser Thr
Phe Ser 275 280 285
Gln Ala Arg Ser Ala Ala Val Gln Ala Ala Ser Asp Leu Tyr Gly Ser 290
295 300 Asn Ser Pro Glu Val
Gln Ser Val Asn Gln Ser Phe Asp Ala Val Gly 305 310
315 320 Ile Asn 63306PRTPaenibacillus
peoriaemisc_feature(1)..(306)PpePro1 63Ala Thr Gly Thr Gly Arg Gly Val
Asp Gly Val Thr Lys Ser Phe Thr 1 5 10
15 Thr Thr Ala Ser Gly Asn Gly Tyr Gln Leu Lys Asp Thr
Thr Arg Ser 20 25 30
Asn Gly Ile Val Thr Tyr Thr Ala Asn Asn Arg Gln Thr Thr Pro Gly
35 40 45 Thr Ile Met Thr
Asp Ala Asp Asn Val Trp Asn Asp Pro Ala Ala Val 50
55 60 Asp Ala His Ala Tyr Ala Ile Lys
Thr Tyr Asp Tyr Tyr Lys Asn Lys 65 70
75 80 Phe Gly Arg Asp Ser Ile Asp Gly Arg Gly Met Gln
Ile Arg Ser Thr 85 90
95 Val His Tyr Gly Lys Lys Tyr Val Asn Ala Phe Trp Asn Gly Ser Gln
100 105 110 Met Thr Tyr
Gly Asp Gly Asp Gly Ser Thr Phe Thr Phe Phe Ser Gly 115
120 125 Asp Pro Asp Val Val Gly His Glu
Leu Thr His Gly Val Thr Glu Phe 130 135
140 Thr Ser Asn Leu Glu Tyr Tyr Gly Glu Ser Gly Ala Leu
Asn Glu Ala 145 150 155
160 Phe Ser Asp Ile Ile Gly Asn Asp Ile Asp Gly Ala Asn Trp Leu Leu
165 170 175 Gly Asp Gly Ile
Tyr Thr Pro Gly Ile Pro Gly Asp Ala Leu Arg Ser 180
185 190 Leu Ser Asp Pro Thr Arg Phe Gly Gln
Pro Asp His Tyr Ser Asn Phe 195 200
205 Tyr Pro Asp Pro Asn Asn Asp Asp Glu Gly Gly Val His Thr
Asn Ser 210 215 220
Gly Ile Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Thr Ser 225
230 235 240 His Gly Val Lys Val
Thr Gly Ile Gly Arg Glu Ala Ala Val Phe Ile 245
250 255 Tyr Tyr Asn Ala Phe Thr Asn Tyr Leu Thr
Ser Thr Ser Asn Phe Ser 260 265
270 Asn Ala Arg Ala Ala Val Ile Gln Ala Ala Lys Asp Phe Tyr Gly
Ala 275 280 285 Asp
Ser Leu Ala Val Thr Ser Ala Ile Lys Ser Phe Asp Ala Val Gly 290
295 300 Ile Lys 305
64304PRTPaenibacillus polymyxamisc_feature(1)..(304)PpoPro2 64Ala Thr Gly
Thr Gly Lys Gly Val Leu Gly Asp Thr Lys Ser Phe Thr 1 5
10 15 Thr Thr Ala Ser Gly Ser Ser Tyr
Gln Leu Lys Asp Thr Thr Arg Gly 20 25
30 Asn Gly Ile Val Thr Tyr Thr Ala Ser Asn Arg Gln Ser
Ile Pro Gly 35 40 45
Thr Leu Leu Thr Asp Ala Asp Asn Val Trp Asn Asp Pro Ala Gly Val 50
55 60 Asp Ala His Ala
Tyr Ala Ala Lys Thr Tyr Asp Tyr Tyr Lys Ser Lys 65 70
75 80 Phe Gly Arg Asp Ser Val Asp Gly Arg
Gly Leu Gln Leu Arg Ser Thr 85 90
95 Val His Tyr Gly Ser Arg Tyr Asn Asn Ala Phe Trp Asn Gly
Ser Gln 100 105 110
Met Thr Tyr Gly Asp Gly Asp Gly Ser Thr Phe Ile Ala Phe Ser Gly
115 120 125 Asp Pro Asp Val
Val Gly His Glu Leu Thr His Gly Val Thr Glu Tyr 130
135 140 Thr Ser Asn Leu Glu Tyr Tyr Gly
Glu Ser Gly Ala Leu Asn Glu Ala 145 150
155 160 Phe Ser Asp Val Ile Gly Asn Asp Ile Gln Arg Lys
Asn Trp Leu Val 165 170
175 Gly Asp Asp Ile Tyr Thr Pro Asn Ile Ala Gly Asp Ala Leu Arg Ser
180 185 190 Met Ser Asn
Pro Thr Leu Tyr Asp Gln Pro Asp His Tyr Ser Asn Leu 195
200 205 Tyr Lys Gly Ser Ser Asp Asn Gly
Gly Val His Thr Asn Ser Gly Ile 210 215
220 Ile Asn Lys Ala Tyr Tyr Leu Leu Ala Gln Gly Gly Thr
Phe His Gly 225 230 235
240 Val Ala Val Asn Gly Ile Gly Arg Asp Ala Ala Val Gln Ile Tyr Tyr
245 250 255 Ser Ala Phe Thr
Asn Tyr Leu Thr Ser Ser Ser Asp Phe Ser Asn Ala 260
265 270 Arg Ala Ala Val Ile Gln Ala Ala Lys
Asp Leu Tyr Gly Ala Asn Ser 275 280
285 Ala Glu Ala Thr Ala Ala Ala Lys Ser Phe Asp Ala Val Gly
Val Asn 290 295 300
65303PRTPaenibacillus terraemisc_feature(1)..(303)PtePro1 65Ala Thr Gly
Thr Gly Val Gly Val Leu Gly Asp Thr Lys Thr Phe Thr 1 5
10 15 Thr Thr Gln Ser Gly Thr Gln Tyr
Val Met Gln Asp Thr Thr Arg Gly 20 25
30 Gly Gly Ile Val Thr Tyr Ser Ala Gly Asn Thr Gln Ser
Leu Pro Gly 35 40 45
Thr Leu Met Arg Asp Thr Asp Asn Val Trp Thr Asp Pro Ala Ala Val 50
55 60 Asp Ala His Ala
Tyr Ala Ala Val Val Tyr Asp Tyr Phe Lys Asn Asn 65 70
75 80 Phe Asn Arg Asp Ser Leu Asp Gly Arg
Gly Met Ala Ile Lys Ser Thr 85 90
95 Val His Tyr Gly Ser Arg Tyr Asn Asn Ala Phe Trp Asn Gly
Thr Gln 100 105 110
Ile Ala Tyr Gly Asp Gly Asp Gly Thr Thr Phe Arg Ala Phe Ser Gly
115 120 125 Asp Leu Asp Val
Ile Gly His Glu Leu Thr His Gly Ile Thr Glu Lys 130
135 140 Thr Ala Gly Leu Ile Tyr Gln Gly
Glu Ser Gly Ala Leu Asn Glu Ser 145 150
155 160 Ile Ser Asp Val Phe Gly Asn Thr Ile Gln Gly Lys
Asn Trp Leu Ile 165 170
175 Gly Asp Asp Ile Tyr Thr Pro Ser Ile Pro Gly Asp Ala Leu Arg Ser
180 185 190 Met Glu Asn
Pro Thr Leu Phe Asn Gln Pro Asp His Tyr Ser Asn Ile 195
200 205 Tyr Arg Gly Ser Asp Asp Asn Gly
Gly Val His Thr Asn Ser Gly Ile 210 215
220 Pro Asn Lys Ala Phe Tyr Leu Leu Ala Gln Gly Gly Thr
His Arg Gly 225 230 235
240 Val Ser Val Thr Gly Ile Gly Arg Gly Asp Ala Ala Lys Ile Val Tyr
245 250 255 Lys Ala Leu Thr
Tyr Tyr Leu Thr Ser Thr Ser Asn Phe Ala Ala Met 260
265 270 Arg Gln Ala Ala Ile Ser Ser Ala Thr
Asp Leu Phe Gly Ala Asn Ser 275 280
285 Ala Gln Val Asn Ser Val Lys Ala Ala Tyr Ala Ala Val Gly
Ile 290 295 300
66304PRTBrevibacillus brevismisc_feature(1)..(304)BbrPro1 66Val Thr Ala
Thr Gly Lys Gly Val Leu Gly Asp Thr Lys Gln Phe Glu 1 5
10 15 Thr Thr Lys Gln Gly Ser Thr Tyr
Met Leu Lys Asp Thr Thr Arg Gly 20 25
30 Lys Gly Ile Glu Thr Tyr Thr Ala Asn Asn Arg Thr Ser
Leu Pro Gly 35 40 45
Thr Leu Met Thr Asp Ser Asp Asn Tyr Trp Thr Asp Gly Ala Ala Val 50
55 60 Asp Ala His Ala
His Ala Gln Lys Thr Tyr Asp Tyr Phe Arg Asn Val 65 70
75 80 His Asn Arg Asn Ser Tyr Asp Gly Asn
Gly Ala Val Ile Arg Ser Thr 85 90
95 Val His Tyr Ser Thr Arg Tyr Asn Asn Ala Phe Trp Asn Gly
Ser Gln 100 105 110
Met Val Tyr Gly Asp Gly Asp Gly Thr Thr Phe Leu Pro Leu Ser Gly
115 120 125 Gly Leu Asp Val
Val Ala His Glu Leu Thr His Ala Val Thr Glu Arg 130
135 140 Thr Ala Gly Leu Val Tyr Gln Asn
Glu Ser Gly Ala Leu Asn Glu Ser 145 150
155 160 Met Ser Asp Ile Phe Gly Ala Met Val Asp Asn Asp
Asp Trp Leu Met 165 170
175 Gly Glu Asp Ile Tyr Thr Pro Gly Arg Ser Gly Asp Ala Leu Arg Ser
180 185 190 Leu Gln Asp
Pro Ala Ala Tyr Gly Asp Pro Asp His Tyr Ser Lys Arg 195
200 205 Tyr Thr Gly Ser Gln Asp Asn Gly
Gly Val His Thr Asn Ser Gly Ile 210 215
220 Asn Asn Lys Ala Ala Tyr Leu Leu Ala Glu Gly Gly Thr
His Tyr Gly 225 230 235
240 Val Arg Val Asn Gly Ile Gly Arg Thr Asp Thr Ala Lys Ile Tyr Tyr
245 250 255 His Ala Leu Thr
His Tyr Leu Thr Pro Tyr Ser Asn Phe Ser Ala Met 260
265 270 Arg Arg Ala Ala Val Leu Ser Ala Thr
Asp Leu Phe Gly Ala Asn Ser 275 280
285 Arg Gln Val Gln Ala Val Asn Ala Ala Tyr Asp Ala Val Gly
Val Lys 290 295 300
67300PRTBacillus subtilismisc_feature(1)..(300)NprE 67Ala Ala Thr Thr Gly
Thr Gly Thr Thr Leu Lys Gly Lys Thr Val Ser 1 5
10 15 Leu Asn Ile Ser Ser Glu Ser Gly Lys Tyr
Val Leu Arg Asp Leu Ser 20 25
30 Lys Pro Thr Gly Thr Gln Ile Ile Thr Tyr Asp Leu Gln Asn Arg
Glu 35 40 45 Tyr
Asn Leu Pro Gly Thr Leu Val Ser Ser Thr Thr Asn Gln Phe Thr 50
55 60 Thr Ser Ser Gln Arg Ala
Ala Val Asp Ala His Tyr Asn Leu Gly Lys 65 70
75 80 Val Tyr Asp Tyr Phe Tyr Gln Lys Phe Asn Arg
Asn Ser Tyr Asp Asn 85 90
95 Lys Gly Gly Lys Ile Val Ser Ser Val His Tyr Gly Ser Arg Tyr Asn
100 105 110 Asn Ala
Ala Trp Ile Gly Asp Gln Met Ile Tyr Gly Asp Gly Asp Gly 115
120 125 Ser Phe Phe Ser Pro Leu Ser
Gly Ser Met Asp Val Thr Ala His Glu 130 135
140 Met Thr His Gly Val Thr Gln Glu Thr Ala Asn Leu
Asn Tyr Glu Asn 145 150 155
160 Gln Pro Gly Ala Leu Asn Glu Ser Phe Ser Asp Val Phe Gly Tyr Phe
165 170 175 Asn Asp Thr
Glu Asp Trp Asp Ile Gly Glu Asp Ile Thr Val Ser Gln 180
185 190 Pro Ala Leu Arg Ser Leu Ser Asn
Pro Thr Lys Tyr Gly Gln Pro Asp 195 200
205 Asn Phe Lys Asn Tyr Lys Asn Leu Pro Asn Thr Asp Ala
Gly Asp Tyr 210 215 220
Gly Gly Val His Thr Asn Ser Gly Ile Pro Asn Lys Ala Ala Tyr Asn 225
230 235 240 Thr Ile Thr Lys
Ile Gly Val Asn Lys Ala Glu Gln Ile Tyr Tyr Arg 245
250 255 Ala Leu Thr Val Tyr Leu Thr Pro Ser
Ser Thr Phe Lys Asp Ala Lys 260 265
270 Ala Ala Leu Ile Gln Ser Ala Arg Asp Leu Tyr Gly Ser Gln
Asp Ala 275 280 285
Ala Ser Val Glu Ala Ala Trp Asn Ala Val Gly Leu 290
295 300 68300PRTBacillus
subtilismisc_feature(1)..(300)NprE_variant 68Ala Ala Thr Thr Gly Thr Gly
Thr Thr Leu Lys Gly Lys Thr Val Ser 1 5
10 15 Leu Asn Ile Ser Ser Glu Ser Gly Lys Tyr Val
Leu Arg Asp Leu Ser 20 25
30 Lys Pro Thr Gly Thr Gln Ile Ile Thr Tyr Asp Leu Gln Asn Arg
Glu 35 40 45 Tyr
Asn Leu Pro Gly Thr Leu Val Ser Ser Thr Thr Asn Gln Phe Thr 50
55 60 Thr Ser Ser Gln Arg Ala
Ala Val Asp Ala His Tyr Asn Leu Gly Lys 65 70
75 80 Val Tyr Asp Tyr Phe Tyr Gln Lys Phe Asn Arg
Asn Ser Tyr Asp Asn 85 90
95 Lys Gly Gly Lys Ile Val Ser Ser Val His Tyr Gly Ser Arg Tyr Asn
100 105 110 Asn Ala
Ala Trp Ile Gly Asp Gln Met Ile Tyr Gly Asp Gly Asp Gly 115
120 125 Ile Leu Phe Ser Pro Leu Ser
Gly Ser Leu Asp Val Thr Ala His Glu 130 135
140 Met Thr His Gly Val Thr Gln Glu Thr Ala Asn Leu
Asn Tyr Glu Asn 145 150 155
160 Gln Pro Gly Ala Leu Asn Glu Ser Phe Ser Asp Val Phe Gly Tyr Phe
165 170 175 Asn Asp Thr
Glu Asp Trp Asp Ile Gly Glu Asp Ile Thr Ile Ser Gln 180
185 190 Pro Ala Leu Arg Ser Leu Ser Asn
Pro Thr Lys Tyr Gly Gln Pro Asp 195 200
205 Asn Phe Lys Asn Tyr Lys Asn Leu Pro Asn Thr Pro Ala
Gly Asp Tyr 210 215 220
Gly Gly Val His Thr Asn Ser Gly Ile Pro Asn Lys Ala Ala Tyr Asn 225
230 235 240 Thr Ile Thr Lys
Ile Gly Val Asn Lys Ala Glu Gln Ile Tyr Tyr Arg 245
250 255 Ala Leu Thr Val Tyr Leu Thr Pro Ser
Ser Thr Phe Lys Asp Ala Lys 260 265
270 Ala Ala Leu Ile Gln Ser Ala Arg Asp Leu Tyr Gly Ser Gln
Asp Ala 275 280 285
Ala Ser Val Glu Ala Ala Trp Asn Ala Val Gly Leu 290
295 300
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