INDUCIBLE PROMOTER SEQUENCES FOR REGULATED EXPRESSION AND METHODS OF USE

Abstract

The plant promoter of a CBSU-Anther_Subtraction library (CAS1) gene encoding a mannitol dehydrogenase, and fragments thereof, and their use in promoting the expression of one or more heterologous nucleic acid fragments in an inducible manner in plants are described. These promoter fragments are also useful in creating recombinant DNA constructs comprising nucleic acid sequences encoding a desired gene product operably linked to such promoter fragments which can be utilized to transform plants and bring the expression of the gene product under external chemical and/or heat control in monocotyledonous and dicotyledonous plants.

Description

[0001] This application claims the benefit of U.S. Provisional Application No. 61/648,758, filed May 18, 2012, the entire content of which is herein incorporated by reference.

FIELD OF THE INVENTION

[0002] The present invention relates to a plant promoter, and fragments thereof, and their use in altering expression of at least one heterologous nucleic acid sequence in plants in an inducible manner. These promoter fragments are also useful in creating recombinant DNA constructs comprising nucleic acid sequences encoding a desired gene product operably linked to such promoter fragments which can be utilized to transform plants and bring the expression of the gene product under external chemical and/or stress control in monocotyledonous and dicotyledonous plants.

BACKGROUND OF THE INVENTION

[0003] Recent advances in plant genetic engineering have opened new doors to engineer plants to have improved characteristics or traits, such as plant disease resistance, insect resistance, herbicidal resistance, yield improvement, improvement of the nutritional quality of the edible portions of the plant, and enhanced stability or shelf-life of the ultimate consumer product obtained from the plants. Thus, a desired gene (or genes) with the molecular function to impart different or improved characteristics or qualities, can be incorporated properly into the plant's genome. The newly integrated gene (or genes) coding sequence can then be expressed in the plant cell to exhibit the desired new trait or characteristics. It is important that appropriate regulatory signals must be present in proper configurations in order to obtain the expression of the newly inserted gene coding sequence in the plant cell. These regulatory signals typically include a promoter region, a 5' non-translated leader sequence and a 3' transcription termination/polyadenylation sequence.

[0004] A promoter is a non-coding genomic DNA sequence, usually upstream (5') to the relevant coding sequence, to which RNA polymerase binds before initiating transcription. This binding aligns the RNA polymerase so that transcription will initiate at a specific transcription initiation site. The nucleotide sequence of the promoter determines the nature of the enzyme and other related protein factors that attach to it and the rate of RNA synthesis. The RNA is processed to produce messenger RNA (mRNA) which serves as a template for translation of the RNA sequence into the amino acid sequence of the encoded polypeptide. The 5' non-translated leader sequence is a region of the mRNA upstream of the coding region that may play a role in initiation and translation of the mRNA. The 3' transcription termination/polyadenylation signal is a non-translated region downstream of the coding region that functions in the plant cell to cause termination of the RNA synthesis and the addition of polyadenylate nucleotides to the 3' end.

[0005] It has been shown that certain promoters are able to direct RNA synthesis at a higher rate than others. These are called "strong promoters". Certain other promoters have been shown to direct RNA synthesis at higher levels only in particular types of cells or tissues and are often referred to as "tissue specific promoters", or "tissue-preferred promoters" if the promoters direct RNA synthesis preferably in certain tissues but also in other tissues at reduced levels. Certain promoters are able to direct RNA synthesis at relatively similar levels across all tissues of a plant. These are called "constitutive promoters" or "tissue-independent" promoters. Constitutive promoters can be divided into strong, moderate and weak according to their effectiveness to direct RNA synthesis. In some cases promoters are able to direct RNA synthesis when they are induced by external stimuli such as chemicals, stress, or biotic stimuli. These are called "inducible promoters".

[0006] The ability to externally control the expression of selected genes and thereby their gene products in plant cells and/or field grown plants can provide important agronomic and foodstuff benefits. This control is desirable for the regulation of genes that might be placed into transgenic plants and has many applications including, but not limited to, (1) prolonging or extending the accumulation of desirable nutritional food reserve in seeds, roots, (2) producing and accumulating products in plant tissues at a defined time in the developmental cycle such that these products are convenient for harvest and/or isolation, and (3) initiating the expression a pest-specific toxin at the site of pathogen attack. There is an ongoing interest in the isolation of novel inducible promoters which are capable of controlling the expression of a chimeric gene or (genes) at certain levels in a plant cell when exposed to external stimuli.

SUMMARY OF THE INVENTION

[0007] This invention relates to a plant promoter of a CBSU-Anther_Subtraction library (CAS1) gene encoding a mannitol dehydrogenase, and functional fragments thereof, and their use in promoting the expression of one or more heterologous nucleic acid fragments in an inducible manner in plants. These promoter fragments are also useful in creating recombinant DNA constructs comprising nucleic acid sequences encoding a desired gene product operably linked to such promoter fragments which can be utilized to transform plants and bring the expression of the gene product under external chemical and/or heat control in monocotyledonous and dicotyledonous plants. One embodiment of the invention concerns an isolated nucleic acid fragment comprising an inducible ZmCAS1 promoter wherein said promoter consists essentially of the nucleotide sequence set forth in SEQ ID NOs: 9 or 10, or said promoter consists essentially of a fragment that is substantially similar and functionally equivalent to the nucleotide sequence set forth in SEQ ID NOs: 9 or 10. The ZmCAS1 promoter can be induced by a chemical or stress treatment. The chemical can be a safener such as, but not limited to, N-(aminocarbonyl)-2-chlorobenzenesulfonamide (2-CBSU). The stress treatment can be a treatment such as, but not limited to, a heat shock treatment of a temperature greater than 26° C.

[0008] The invention also concerns a recombinant DNA construct comprising at least one heterologous nucleic acid fragment operably linked to the promoter of the invention.

[0009] In another embodiment, this invention concems a cell, plant, or seed comprising a recombinant expression construct of the present disclosure.

[0010] In another embodiment, this invention concerns a plant stably transformed with a recombinant expression construct comprising a plant promoter and a heterologous nucleic acid fragment operably linked to said promoter, wherein said promoter is an inducible promoter and capable of controlling expression of said heterologous nucleic acid fragment in a plant cell, and further wherein said promoter comprises a fragment of SEQ ID NOs: 9 or 10.

[0011] In another embodiment, this invention concerns a method of expressing a coding sequence or a functional RNA in a plant cell comprising: a) introducing the recombinant DNA construct of the current disclosure into a plant cell, wherein at least one heterologous sequence comprises a coding sequence or a functional RNA, b) growing the plant cell of step a); c) induction of the inducible promoter by chemical or stress treatment on the plant cell of b); and, d) selecting a plant cell displaying expression of the coding sequence or the functional RNA of the recombinant DNA construct. In another embodiment, this invention concerns a method of expressing a coding sequence or a functional RNA driven by the promoter of the current invention in anther, callus, leaf or root cells.

BRIEF DESCRIPTION OF THE DRAWINGS AND SEQUENCE LISTINGS

[0012] The invention can be more fully understood from the following detailed description and the accompanying drawings and Sequence Listing that form a part of this application.

[0013] FIG. 1: Alignment the amino acid sequence encoded by the ZmCAS1cDNA (SEQ ID NO:5) with a maize mannitol dehydrogenase (GI:226528549; SEQ ID NO:6) (A) and percent identity (B).

[0014] FIG. 2: Norther blot of maize anther RNA of wild-type fertile (F) and sterile (S) maize control plants (-) and maize CBSU treated plants (+). Maize anther RNA was analyzed with probes specific for ZmCAS1, IN2-2, 5126, MS45, ACTIN and UBI gene expression.

[0015] FIG. 3: Northern blot of maize callus (C), leaf (L) and anther (A) RNAs from wild-type maize tissues and CBSU-treated (+) tissues. Maize RNA was analyzed with probes specific for IN2-2 and ZmCAS1.

[0016] FIG. 4 shows A) maize callus transformed with PHP16975 comprising the 1.7 kb ZmCAS1 promoter for three different events (1, 2, 3) C=control maintenance media; 10=10 mg/l CBSU, 100=100 mg/l CBSU; B) maize callus transformed with PHP16974 comprising the truncated 1.0 kb ZmCAS1 promoter and induced with either CBSU or heat (37° C.); 26C=control callus at 26° C.; 26C+CBSU=CBSU treated callus at 26° C.; 37C=callus induced by heat treatment of 37° C. Results from seven events (1-7) are shown.

[0017] FIG. 5 shows maize leaf punches from three (1, 2, 3) maize plants transformed with PHP16975 and induced with CBSU. Leaf punches from plants regenerated from the 3 bialophos-resistant events were collected pre- (C) and post-watering (S).

[0018] FIG. 6 shows a Northern blot of maize callus RNA from five (1, 2, 3, 4 and 5) events transformed with PHP16972 and treated with (+) or without (-) CBSU.

[0019] FIG. 7 shows a Western analysis of leaves from ms45/ms45 maize plants transformed with PHP16973 using antibodies directed against the maize MS45 protein. C=leaves from uninduced control plants, +=leaves from CBSU induced plants. Whole-cell anther extract from a wild-type MS45 plant is shown in Lane 1 and used to identify the mobility of the immunoreactive MS45 protein as indicated by the arrow.

[0020] FIG. 8 shows a Western analysis of anthers from ms45/ms45 maize plants transformed with PHP16973 using antibodies directed against the maize MS45 protein. C=leaves from uninduced control plants, +=leaves from CBSU induced plants.

[0021] FIG. 9: Rice events transformed with PHP16974 show GUS expression when driven by the 1.0 kb ZmCAS1 promoter and induced by CBSU.

[0022] FIG. 10: Rice seedlings transformed with PHP16974 show GUS expression when driven by the 1.0 kb ZmCAS1 promoter and induced by CBSU.

[0023] The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.

[0024] The sequence descriptions summarize the Sequence Listing attached hereto. The Sequence Listing contains one letter codes for nucleotide sequence characters and the single and three letter codes for amino acids as defined in the IUPAC-IUB standards described in Nucleic Acids Research 13:3021-3030 (1985) and in the Biochemical Journal 219(2):345-373 (1984). The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. §1.822.

[0025] SEQ ID NO:1 DNA insert comprising the ZmCAS1c-1 cDNA.

[0026] SEQ ID NO:2 DNA insert comprising the ZmCAS1c-2 cDNA.

[0027] SEQ ID NO:3 A 1354 bp (base pair) SalI-NotI DNA insert comprising the maize ZmCAS1 full length cDNA.

[0028] SEQ ID NO:4 the 1338 bp maize ZmCAS1 full length cDNA.

[0029] SEQ ID NO:5 the amino acid sequence encoded by SEQ ID NO:4

[0030] SEQ ID NO:6 the amino acid sequence of a maize mannitol dehydrogenase (GI number 226528549, NP_--001147757.1)

[0031] SEQ ID NO:7 a 4069 bp DNA fragment comprising the maize B73 ZmCAS1 promoter

[0032] SEQ ID NO:8 is the DNA sequence of the oligonucleotide used for mutagenesis to introduce RCAI DNA restriction site.

[0033] SEQ ID NO:9 is a 1049 bp truncated form of the maize ZmCAS1 promoter (bp 698-1746 of SEQ ID NO:9) also referred to as the 1.0 kb ZmCAS1 promoter.

[0034] SEQ ID NO:10 is 1746 bp maize ZmCAS1 promoter, also referred to as the 1.7 kb ZmCAS1 promoter.

[0035] SEQ ID NO: 11 is the nucleotide sequence of PHP16974 comprising the 1.0 kb ZmCAS1 promoter.

[0036] SEQ ID NO: 12 is the nucleotide sequence of PHP16975 comprising the 1.7 kb ZmCAS1 promoter.

[0037] SEQ ID NO: 13 is the nucleotide sequence of PHP16972 comprising the 1.0 kb ZmCAS1 promoter.

[0038] SEQ ID NO: 14 is the nucleotide sequence of PHP16973 comprising the 1.7 kb ZmCAS1 promoter.

[0039] SEQ ID NO: 15 is the HindIII-Rca1 fragment (ZMCAS1HINDIIIPRO) comprising the 1.0 kb ZmCAS1 promoter of SEQ ID NO:9.

[0040] SEQ ID NO: 16 is the BamH1-Rca1 fragment (ZMCAS1BAMPRO) comprising the 1.7 kb ZmCAS1 promoter of SEQ ID NO:10.

[0041] SEQ ID NO: 17 is the amino acid sequence of a mannitol dehydrogenase (AAP52597) from rice (Oryza sativa).

[0042] SEQ ID NO: 18 is a nudeotide sequence from a mannitol dehydrogenase gene region (DP000086) from rice (Oryza sativa).

[0043] SEQ ID NO: 19 is a nucleotide sequence of a putative 5'UTR-Promoter region from a mannitol dehydrogenase gene (DP000086) from rice (Oryza sativa).

[0044] SEQ ID NO: 20 is the amino acid sequence of a mannitol dehydrogenase (XP-002436634) from Sorghum.

[0045] SEQ ID NO: 21 is a nucleotide sequence from a mannitol dehydrogenase gene region (NC-012879) from Sorghum.

[0046] SEQ ID NO: 22 is a nucleotide sequence of a putative 5'UTR-Promoter region from a mannitol dehydrogenase gene (NC-012879) from Sorghum.

DETAILED DESCRIPTION OF THE INVENTION

[0047] The disclosure of all patents, patent applications, and publications cited herein are incorporated by reference in their entirety.

[0048] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Unless mentioned otherwise, the techniques employed or contemplated herein are standard methodologies well known to one of ordinary skill in the art. The materials, methods and examples are illustrative only and not limiting.

[0049] As used herein and in the appended claims, the singular forms "a", "an", and "the" include plural reference unless the context clearly dictates otherwise. Thus, for example, reference to "a plant" includes a plurality of such plants, reference to "a cell" includes one or more cells and equivalents thereof known to those skilled in the art, and so forth.

[0050] In the context of this disclosure, a number of terms shall be utilized. As used herein, a "ZmCAS1 promoter" refers to one type of inducible promoter. The native ZmCAS1 promoter is the promoter of a maize gene isolated from a CBSU-Anther_Subtraction library with significant homology to mannitol dehydrogenase genes identified in various plant species including maize that are deposited in National Center for Biotechnology Information (NCBI) database. The "ZmCAS1 promoter", as used herein, also refers to fragments of the full-length native promoter that retain significant promoter activity. For example, a ZmCAS1 promoter can be 1.7 kb in length (SEQ ID NO: 10) or a promoter-functioning fragment thereof, which includes, among others, the polynucleotide of SEQ ID NO: 9. A ZmCAS1 promoter also includes variants that are substantially similar and functionally equivalent to any portion of the nucleotide sequence, in increments of one base pair, between the 1.0 kb (SQE ID NO:9) and 1.7 kb (SEQ ID NO:10) fragments and sequences.

[0051] The term "Promoter" refers to a nucleotide sequence capable of regulating the expression of a coding sequence or functional RNA. Functional RNA includes, but is not limited to, transfer RNA (tRNA) and ribosomal RNA (rRNA). The promoter sequence consists of proximal and more distal upstream elements, the latter elements often referred to as enhancers. The promoter usually comprises a TATA box capable of directing RNA polymerase II to initiate RNA synthesis at the appropriate transcription initiation site for a particular coding sequence. A promoter can additionally comprise other recognition sequences generally positioned upstream or 5' to the TATA box, referred to as upstream promoter elements, which influence the transcription initiation rate. It is recognized that having identified the nucleotide sequences for the promoter region disclosed herein, it is within the state of the art to isolate and identify further regulatory elements in the region upstream of the TATA box from the particular promoter region identified herein. Accordingly, an "enhancer" is a DNA sequence which can stimulate promoter activity and may be an innate element of the promoter or a heterologous element inserted to enhance the level or tissue-specificity of a promoter. Promoters may be derived in their entirety from a native gene, or be composed of different elements derived from different promoters found in nature, or even comprise synthetic DNA segments. It is understood by those skilled in the art that different promoters may direct the expression of a gene in different tissues or cell types, or at different stages of development, or in response to different environmental or abiotic conditions.

[0052] The promoter elements which enable the inducible expression in the desired tissue can be identified, isolated, and used with other core promoters to confirm inducible expression. By core promoter is meant the minimal sequence required to initiate transcription, such as the sequence called the TATA box which is common to promoters in genes encoding proteins. Thus, the ZmCAS1 promoter can optionally be used in conjunction with its own or core promoters from other sources. The promoter may be native or non-native to the cell in which it is found.

[0053] Promoters which cause a gene to be expressed in most cell types at most times are commonly referred to as "constitutive promoters". New promoters of various types useful in plant cells are constantly being discovered; numerous examples may be found in the compilation by Okamuro and Goldberg (Biochemistry of Plants 15:1-82 (1989)). It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, DNA fragments of some variation may have identical promoter activity.

[0054] High level, constitutive expression of the candidate gene under control of the 35S or UBI promoter may have pleiotropic effects, although candidate gene efficacy may be estimated when driven by a constitutive promoter. Use of inducible or stress-specific promoters may eliminate undesirable effects but retain the ability to enhance drought tolerance. This effect has been observed in Arabidopsis (Kasuga et al. (1999) Nature Biotechnol. 17:287-91).

[0055] The term "inducible promoter" refers to promoters that selectively express a coding sequence or functional RNA in response to the presence of an endogenous or exogenous stimulus, for example by chemical compounds (chemical inducers) or in response to environmental, hormonal, chemical, and/or developmental signals. Inducible or regulated promoters include, for example, promoters induced or regulated by light, heat, stress, flooding or drought, salt stress, osmotic stress, phytohormones, wounding, or chemicals such as ethanol, abscisic acid (ABA), jasmonate, salicylic acid, or safeners.

[0056] An example of a stress-inducible is RD29A promoter (Kasuga et al. (1999) Nature Biotechnol. 17:287-91). One of ordinary skill in the art is familiar with protocols for simulating drought conditions and for evaluating drought tolerance of plants that have been subjected to simulated or naturally-occurring drought conditions. For example, one can simulate drought conditions by giving plants less water than normally required or no water over a period of time, and one can evaluate drought tolerance by looking for differences in physiological and/or physical condition, including (but not limited to) vigor, growth, size, or root length, or in particular, leaf color or leaf area size. Other techniques for evaluating drought tolerance include measuring chlorophyll fluorescence, photosynthetic rates and gas exchange rates. Also, one of ordinary skill in the art is familiar with protocols for simulating stress conditions such as osmotic stress, salt stress and temperature stress and for evaluating stress tolerance of plants that have been subjected to simulated or naturally-occurring stress conditions.

[0057] The sequences of the invention may be isolated from any plant, including, but not limited to corn (Zea mays), canola (Brassica napus, Brassica rapa ssp.), alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), sunflower (Helianthus annuus), wheat (Triticum aestivum), soybean (Glycine max), tobacco (Nicotiana tabacum), millet (Panicum spp.), potato (Solanum tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium hirsutum), sweet potato (Ilpomoea batatus), cassava (Manihot esculenta), coffee (Cofea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Persea americana), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), oats (Avena sativa), barley (Hordeum vulgare), vegetables, ornamentals, and conifers. Preferably, plants include corn, soybean, sunflower, safflower, canola, wheat, barley, rye, alfalfa, rice, cotton and sorghum.

[0058] This invention concerns an isolated nucleic acid fragment comprising an inducible ZmCAS1 promoter. This invention also concerns an isolated nucleic acid fragment comprising a promoter wherein said promoter consists essentially of the nucleotide sequence set forth in SEQ ID NO:9, or said promoter consists essentially of a fragment that is substantially similar and functionally equivalent to the nucleotide sequence set forth in SEQ ID NO:10. A nucleic acid fragment that is functionally equivalent to the instant ZmCAS1 promoter is any nucleic acid fragment that is capable of controlling the expression of a coding sequence or functional RNA in a similar manner to the ZmCAS1 promoter. The expression patterns of ZmCAS1 gene and its promoter are set forth in Examples 1-3.

[0059] The promoter activity of the maize genomic DNA fragment SEQ ID NO:9 or SEQ ID NO:10 upstream of the ZmCAS1 protein coding sequence was assessed by linking the fragment to a GUS gene or a MS45 gene, transforming the promoter:GUS (or MS45) expression cassette into maize, and analyzing GUS (or MS45) expression in various cell types of the transgenic plants (Examples 1-3). These results indicated that the nucleic acid fragment contained an inducible promoter.

[0060] In one embodiment, the invention is an isolated polynucleotide comprising, or consisting essentially of or consisting of:

[0061] a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:9 or a full-length complement thereof;

[0062] b) a nucleotide sequence comprising a fragment of SEQ ID NO:10, or a full-length complement thereof

[0063] c) a nucleotide sequence comprising a sequence having at least 90% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a) or (b);

[0064] d) a nucleotide sequence comprising all or a fragment of a 1.7 kb 5' non-coding sequence of a mannitol dehydrogenase; or,

[0065] e) a derivative of one of the nucleotide sequences indicated in (a), (b), or (c) obtained by substitution, addition and/or deletion of one or more nucleotides; and,

[0066] wherein said nucleotide sequence is an inducible promoter.

[0067] In another embodiment of the invention the ZmCAS1 promoter is induced by a safener treatment of N-(aminocarbonyl)-2-chlorobenzenesulfonamide (2-CBSU). In another embodiment of the invention the ZmCAS1 promoter is induced by a heat treatment of a temperature greater than 26° C. and up to and including 37° C.

[0068] The terms "N-(aminocarbonyl)-2-chlorobenzenesulfonamide", 2-CBSU" and "CBSU" are used interchangeably herein.

[0069] The promoter nucleotide sequences and methods disclosed herein are useful in regulating inducible expression of any heterologous nucleotide sequences in a host plant in order to alter the phenotype of a plant.

[0070] Various changes in phenotype are of interest including, but not limited to, modifying the fatty acid composition in a plant, altering the amino acid content of a plant, altering a plant's pathogen defense mechanism, and the like. These results can be achieved by providing expression of heterologous products or increased expression of endogenous products in plants. Alternatively, the results can be achieved by providing for a reduction of expression of one or more endogenous products, particularly enzymes or cofactors in the plant. These changes result in a change in phenotype of the transformed plant.

[0071] Genes of interest are reflective of the commercial markets and interests of those involved in the development of the crop. Crops and markets of interest change, and as developing nations open up world markets, new crops and technologies will emerge also. In addition, as our understanding of agronomic characteristics and traits such as yield and heterosis increase, the choice of genes for transformation will change accordingly. General categories of genes of interest include, but are not limited to, those genes involved in information, such as zinc fingers, those involved in communication, such as kinases, and those involved in housekeeping, such as heat shock proteins. Other gene of interest are genes allowing for site specific gene integration and gene stacking include, but not limited to, double-strand break inducing genes and recombinase genes. More specific categories of transgenes, for example, include, but are not limited to, genes encoding important traits for agronomics, insect resistance, disease resistance, herbicide resistance, sterility, grain or seed characteristics, and commercial products. Genes of interest include, generally, those involved in oil, starch, carbohydrate, or nutrient metabolism as well as those affecting seed size, plant development, plant growth regulation, and yield improvement. Plant development and growth regulation also refer to the development and growth regulation of various parts of a plant, such as the flower, seed, root, leaf and shoot.

[0072] Other commercially desirable traits are genes and proteins conferring cold, heat, salt, and drought resistance.

[0073] One embodiment of the invention relates to a recombinant DNA comprising the isolated polynucleotide of the invention operably linked to at least one heterologous nucleic acid sequence, wherein the heterologous nucleic acid sequence codes for a gene selected from the group consisting of: a double-strand break inducing gene, a recombinase gene, a reporter gene, a selection marker, a disease resistance conferring gene, a herbicide resistance conferring gene, an insect resistance conferring gene; a gene involved in carbohydrate metabolism, a gene involved in fatty acid metabolism, a gene involved in amino acid metabolism, a gene involved in plant development, a gene involved in plant growth regulation, a gene involved in yield improvement, a gene involved in drought resistance, a gene involved in cold resistance, a gene involved in heat and salt resistance in plants.

[0074] Another embodiment of the invention relates to a recombinant DNA comprising the isolated polynucleotide of the invention operably linked to at least one heterologous nucleic acid sequence, wherein the heterologous nucleic acid sequence encodes a protein selected from the group consisting of: a double-strand break inducing protein, a recombinase protein, a reporter protein, a selection marker, a protein conferring disease resistance, protein conferring herbicide resistance, protein conferring insect resistance; protein involved in carbohydrate metabolism, protein involved in fatty acid metabolism, protein involved in amino acid metabolism, protein involved in plant development, protein involved in plant growth regulation, protein involved in yield improvement, protein involved in drought resistance, protein involved in cold resistance, protein involved in heat resistance and salt resistance in plants.

[0075] One embodiment of the invention, comprises a plant (for example, maize or a soybean plant) comprising in its genome a recombinant DNA construct comprising a polynucleotide operably linked to a promoter fragment of the invention, wherein said promoter fragment comprises at least 40%, 45%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO: 9 or 10, and wherein said plant exhibits an alteration of at least one agronomic characteristic when compared to a control plant not comprising said recombinant DNA construct.

[0076] Another embodiment of the invention, comprises a plant (for example, maize or a soybean plant) comprising in its genome a suppression DNA construct comprising a promoter fragment of the invention, wherein said promoter fragment comprises at least 40%, 45%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NOs: 9 or 10, and wherein said plant exhibits an alteration of at least one agronomic characteristic when compared to a control plant not comprising said recombinant DNA construct.

[0077] In any of the foregoing embodiments or any other embodiments of the present invention, the at least one agronomic characteristic may be selected from the group consisting of greenness, yield, growth rate, biomass, fresh weight at maturation, dry weight at maturation, fruit yield, seed yield, total plant nitrogen content, fruit nitrogen content, seed nitrogen content, nitrogen content in a vegetative tissue, total plant free amino acid content, fruit free amino acid content, seed free amino acid content, free amino acid content in a vegetative tissue, total plant protein content, fruit protein content, seed protein content, protein content in a vegetative tissue, drought tolerance, nitrogen uptake, root lodging, harvest index, stalk lodging, plant height, ear height, ear length, early seedling vigor and seedling emergence under low temperature stress. For example, the alteration of at least one agronomic characteristic may be an increase in yield, greenness or biomass.

[0078] Disease and/or insect resistance genes may encode resistance to pests that have great yield drag such as for example, anthracnose, soybean mosaic virus, soybean cyst nematode, root-knot nematode, brown leaf spot, Downy mildew, purple seed stain, seed decay and seedling diseases caused commonly by the fungi--Pythium sp., Phytophthora sp., Rhizoctonia sp., Diaporthe sp. Bacterial blight caused by the bacterium Pseudomonas syringae pv. Glycinea. Genes conferring insect resistance include, for example, Bacillus thuringiensis toxic protein genes (U.S. Pat. Nos. 5,366,892; 5,747,450; 5,737,514; 5,723,756; 5,593,881; and Geiser et al (1986) Gene 48:109); lectins (Van Damme et al. (1994) Plant Mol. Biol. 24:825); and the like.

[0079] Herbicide resistance traits may include genes coding for resistance to herbicides that act to inhibit the action of acetolactate synthase (ALS), in particular the sulfonylurea-type herbicides (e.g., the acetolactate synthase ALS gene containing mutations leading to such resistance, in particular the S4 and/or HRA mutations). The ALS-gene mutants encode resistance to the herbicide chlorsulfuron. Glyphosate acetyl transferase (GAT) is an N-acetyltransferase from Bacillus licheniformis that was optimized by gene shuffling for acetylation of the broad spectrum herbicide, glyphosate, forming the basis of a novel mechanism of glyphosate tolerance in transgenic plants (Castle et al. (2004) Science 304, 1151-1154).

[0080] Antibiotic resistance genes include, for example, neomycin phosphotransferase (npt) and hygromycin phosphotransferase (hpt). Two neomycin phosphotransferase genes are used in selection of transformed organisms: the neomycin phosphotransferase I (nptI) gene and the neomycin phosphotransferase II (nptII) gene. The second one is more widely used. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme, which inactivates by phosphorylation a range of aminoglycoside antibiotics such as kanamycin, neomycin, geneticin and paroromycin. NPTII is widely used as a selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzyme activity. NPTII protein activity can be detected by enzymatic assay. In other detection methods, the modified substrates, the phosphorylated antibiotics, are detected by thin-layer chromatography, dot-blot analysis or polyacrylamide gel electrophoresis. Plants such as maize, cotton, tobacco, Arabidopsis, flax, soybean and many others have been successfully transformed with the nptII gene.

[0081] The hygromycin phosphotransferase (denoted hpt, hph or aphIV) gene was originally derived from Escherichia coli. The gene codes for hygromycin phosphotransferase (HPT), which detoxifies the aminocyclitol antibiotic hygromycin B. A large number of plants have been transformed with the hpt gene and hygromycin B has proved very effective in the selection of a wide range of plants, including monocotyledonous. Most plants exhibit higher sensitivity to hygromycin B than to kanamycin, for instance cereals. Likewise, the hpt gene is used widely in selection of transformed mammalian cells. The sequence of the hpt gene has been modified for its use in plant transformation. Deletions and substitutions of amino acid residues close to the carboxy (C)-terminus of the enzyme have increased the level of resistance in certain plants, such as tobacco. At the same time, the hydrophilic C-terminus of the enzyme has been maintained and may be essential for the strong activity of HPT. HPT activity can be checked using an enzymatic assay. A non-destructive callus induction test can be used to verify hygromycin resistance.

[0082] Genes involved in plant growth and development have been identified in plants. One such gene, which is involved in cytokinin biosynthesis, is isopentenyl transferase (IPT). Cytokinin plays a critical role in plant growth and development by stimulating cell division and cell differentiation (Sun et al. (2003), Plant Physiol. 131: 167-176).

[0083] Calcium-dependent protein kinases (CDPK), a family of serine-threonine kinase found primarily in the plant kingdom, are likely to function as sensor molecules in calcium-mediated signaling pathways. Calcium ions are important second messengers during plant growth and development (Harper et al. Science 252, 951-954 (1993); Roberts et al. Curr Opin Cell Biol 5, 242-246 (1993); Roberts et al. Annu Rev Plant Mol Biol 43, 375-414 (1992)).

[0084] Nematode responsive protein (NRP) is produced by soybean upon the infection of soybean cyst nematode. NRP has homology to a taste-modifying glycoprotein miraculin and the NF34 protein involved in tumor formation and hyper response induction. NRP is believed to function as a defense-inducer in response to nematode infection (Tenhaken et al. BMC Bioinformatics 6:169 (2005)).

[0085] The quality of seeds and grains is reflected in traits such as levels and types of fatty acids or oils, saturated and unsaturated, quality and quantity of essential amino acids, and levels of carbohydrates. Therefore, commercial traits can also be encoded on a gene or genes that could increase for example methionine and cysteine, two sulfur containing amino acids that are present in low amounts in soybeans. Cystathionine gamma synthase (CGS) and serine acetyl transferase (SAT) are proteins involved in the synthesis of methionine and cysteine, respectively.

[0086] Other commercial traits can encode genes to increase for example monounsaturated fatty acids, such as oleic acid, in oil seeds. Soybean oil for example contains high levels of polyunsaturated fatty acids and is more prone to oxidation than oils with higher levels of monounsaturated and saturated fatty acids. High oleic soybean seeds can be prepared by recombinant manipulation of the activity of oleoyl 12-desaturase (Fad2). High oleic soybean oil can be used in applications that require a high degree of oxidative stability, such as cooking for a long period of time at an elevated temperature.

[0087] Raffinose saccharides accumulate in significant quantities in the edible portion of many economically significant crop species, such as soybean (Glycine max L. Merrill), sugar beet (Beta vulgaris), cotton (Gossypium hirsutum L.), canola (Brassica sp.) and all of the major edible leguminous crops including beans (Phaseolus sp.), chick pea (Cicer arietinum), cowpea (Vigna unguiculata), mung bean (Vigna radiata), peas (Pisum sativum), lentil (Lens culinaris) and lupine (Lupinus sp.). Although abundant in many species, raffinose saccharides are an obstacle to the efficient utilization of some economically important crop species.

[0088] Down regulation of the expression of the enzymes involved in raffinose saccharide synthesis, such as galactinol synthase for example, would be a desirable trait.

[0089] In certain embodiments, the present invention contemplates the transformation of a recipient cell with more than one advantageous transgene. Two or more transgenes can be supplied in a single transformation event using either distinct transgene-encoding vectors, or a single vector incorporating two or more gene coding sequences. Any two or more transgenes of any description, such as those conferring herbicide, insect, disease (viral, bacterial, fungal, and nematode) or drought resistance, oil quantity and quality, or those increasing yield or nutritional quality may be employed as desired.

[0090] The term "Anther" or "Anther tissue" refers to male plant tissue encompassing cells, cell-layers and cell types that give rise to pollen grains capable of effecting fertilization. These cells include but are not limited to archesporial cells, pollen mother cells, meiocytes, microspores, tapetum, supporting cell layers, pollen and cells derived from these cell types.

[0091] An "isolated nucleic acid fragment" refers to a polymer of ribonucleotides (RNA) or deoxyribonucleotides (DNA) that is single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases. An isolated nucleic acid fragment in the form of DNA may be comprised of one or more segments of cDNA, genomic DNA or synthetic DNA.

[0092] The terms "polynucleotide", "polynucleotide sequence", "nucleic acid sequence", and "nucleic acid fragment"/"isolated nucleic acid fragment" are used interchangeably herein. These terms encompass nucleotide sequences and the like. A polynucleotide may be a polymer of RNA or DNA that is single- or double-stranded, that optionally contains synthetic, non-natural or altered nucleotide bases. A polynucleotide in the form of a polymer of DNA may be comprised of one or more segments of cDNA, genomic DNA, synthetic DNA, or mixtures thereof. Nucleotides (usually found in their 5'-monophosphate form) are referred to by a single letter designation as follows: "A" for adenylate or deoxyadenylate (for RNA or DNA, respectively), "C" for cytidylate or deoxycytidylate, "G" for guanylate or deoxyguanylate, "U" for uridylate, "T" for deoxythymidylate, "R" for purines (A or G), "Y" for pyrimidines (C or T), "K" for G or T, "H" for A or C or T, "I" for inosine, and "N" for any nucleotide.

[0093] A "heterologous nucleic acid fragment" refers to a sequence that is not naturally occurring with the plant promoter sequence of the invention. While this nucleotide sequence is heterologous to the promoter sequence, it may be homologous, or native, or heterologous, or foreign, to the plant host. However, it is recognized that the instant promoters may be used with their native coding sequences to increase or decrease expression resulting in a change in phenotype in the transformed seed.

[0094] The terms "fragment (or variant) that is functionally equivalent" and "functionally equivalent fragment (or variant)" are used interchangeably herein. These terms refer to a portion or subsequence or variant of the promoter sequence of the present invention in which the ability to initiate transcription or drive gene expression (such as to produce a certain phenotype) is retained. Fragments and variants can be obtained via methods such as site-directed mutagenesis and synthetic construction. As with the provided promoter sequences described herein, the contemplated fragments and variants operate to promote inducible expression of an operably linked heterologous nucleic acid sequence, forming a recombinant DNA construct (also, a chimeric gene). For example, the fragment or variant can be used in the design of recombinant DNA constructs to produce the desired phenotype in a transformed plant. Recombinant DNA constructs can be designed for use in co-suppression or antisense by linking a promoter fragment or variant thereof in the appropriate orientation relative to a heterologous nucleotide sequence.

[0095] A functional fragment of the regulatory sequence can be formed by one or more deletions from a larger sequence. For example, the 5' portion of a promoter up to the TATA box near the transcription start site can be deleted without abolishing promoter activity, as described by Opsahl-Sorteberg, H-G. et al., "Identification of a 49-bp fragment of the HvLTP2 promoter directing aleruone cell specific expression" Gene 341:49-58 (2004). Such variants should retain promoter activity. Activity can be measured by Norther blot analysis, reporter activity measurements when using transcriptional fusions, and the like. See, for example, Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual (2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.), herein incorporated by reference.

[0096] Sequences which hybridize to the regulatory sequences of the present invention are within the scope of the invention. Sequences that correspond to the promoter sequences of the present invention and hybridize to the promoter sequences disclosed herein will be at least 40% homologous, 50% homologous, 70% homologous, and even 85% 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous or more with the disclosed sequence.

[0097] Smaller fragments may yet contain the regulatory properties of the promoter so identified and deletion analysis is one method of identifying essential regions. Deletion analysis can occur from both the 5' and 3' ends of the regulatory region. Fragments can be obtained by site-directed mutagenesis, mutagenesis using the polymerase chain reaction and the like. (See, Directed Mutagenesis: A Practical Approach IRL Press (1991)).

[0098] In some aspects of the present invention, the promoter fragments can comprise at least about 20 contiguous nucleotides, or at least about 50 contiguous nucleotides, or at least about 75 contiguous nucleotides, or at least about 100, 150, 200, 250, 300, 350, 400, 450, 500 contiguous nucleotides of SEQ ID NO:8 or up to the number of nucleotides present in a full-length nucleotide sequence disclosed herein (for example 1746, SEQ ID NO: 10).

[0099] In another aspect, a promoter fragment is the nucleotide sequence set forth in SEQ ID NO: 9 or SEQ ID NO: 10. The nucleotides of such fragments will usually comprise the TATA recognition sequence of the particular promoter sequence. Such fragments may be obtained by use of restriction enzymes to cleave the naturally occurring promoter nucleotide sequences disclosed herein, by synthesizing a nucleotide sequence from the naturally occurring promoter DNA sequence, or may be obtained through the use of PCR technology. See particularly, Mullis et al., Methods Enzymol. 155:335-350 (1987), and Higuchi, R. In PCR Technology: Principles and Applications for DNA Amplifications; Erlich, H. A., Ed.; Stockton Press Inc.: New York, 1989.

[0100] The isolated promoter sequences of the present invention can be modified to provide a range of inducible expression levels of the heterologous nucleotide sequence. Thus, less than the entire promoter regions may be utilized and the ability to drive expression of the coding sequence retained. As described in Examples 1-3, the 1.0 kb ZmCAS1 promoter fragment as well as the longer 1.7 kb ZmCAS1 promoter fragment were able to drive gene expression when induced by a chemical or stress treatment.

[0101] Modifications of the isolated promoter sequences of the present invention can provide for a range of inducible expression of the heterologous nucleotide sequence. Thus, they may be modified to be weak inducible promoters or strong inducible promoters. Generally, by "weak promoter" is intended a promoter that drives expression of a coding sequence at a low level. By "low level" is intended at levels about 1/10,000 transcripts to about 1/100,000 transcripts to about 1/500,000 transcripts. Conversely, a strong promoter drives expression of a coding sequence at high level, or at about 1/10 transcripts to about 1/100 transcripts to about 1/1,000 transcripts.

[0102] The terms "substantially similar" and "corresponding substantially" as used herein refer to nucleic acid fragments wherein changes in one or more nucleotide bases do not affect the ability of the nucleic acid fragment to mediate gene expression or produce a certain phenotype. These terms also refer to modifications of the nucleic acid fragments of the instant invention such as deletion or insertion of one or more nucleotides that do not substantially alter the functional properties of the resulting nucleic acid fragment relative to the initial, unmodified fragment. It is therefore understood, as those skilled in the art will appreciate, that the invention encompasses more than the specific exemplary sequences.

[0103] Moreover, the skilled artisan recognizes that substantially similar nucleic acid sequences encompassed by this invention are also defined by their ability to hybridize, under moderately stringent conditions (for example, 0.5×SSC, 0.1% SDS, 60° C.) with the sequences exemplified herein, or to any portion of the nucleotide sequences reported herein and which are functionally equivalent to the promoter of the invention. Estimates of such homology are provided by either DNA-DNA or DNA-RNA hybridization under conditions of stringency as is well understood by those skilled in the art (Hames and Higgins, Eds.; In Nucleic Acid Hybridisation; IRL Press: Oxford, U.K., 1985). Stringency conditions can be adjusted to screen for moderately similar fragments, such as homologous sequences from distantly related organisms, to highly similar fragments, such as genes that duplicate functional enzymes from closely related organisms. Post-hybridization washes partially determine stringency conditions. One set of conditions uses a series of washes starting with 6×SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2×SSC, 0.5% SDS at 45° C. for 30 min, and then repeated twice with 0.2×SSC, 0.5% SDS at 50° C. for 30 min. Another set of stringent conditions uses higher temperatures in which the washes are identical to those above except for the temperature of the final two 30 min washes in 0.2×SSC, 0.5% SDS was increased to 60° C. Another set of highly stringent conditions uses two final washes in 0.1×SSC, 0.1% SDS at 65° C.

[0104] In general, sequences that correspond to the nucleotide sequences of the present invention and hybridize to the nucleotide sequence disclosed herein will be at least 40% homologous, 50% homologous, 70% homologous, and even 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% homologous or more with the disclosed sequence. That is, the sequence similarity between probe and target may range, sharing at least about 40%, about 50%, about 70%, and even about 85% or more sequence similarity.

[0105] Preferred substantially similar nucleic acid sequences encompassed by this invention are those sequences that are 80% identical to the nucleic acid fragments reported herein or which are 80% identical to any portion of the nucleotide sequences reported herein. More preferred are nucleic acid fragments which are 90% identical to the nucleic acid sequences reported herein, or which are 90% identical to any portion of the nucleotide sequences reported herein. Most preferred are nucleic acid fragments which are 95% identical to the nucleic acid sequences reported herein, or which are 95% identical to any portion of the nucleotide sequences reported herein. It is well understood by one skilled in the art that many levels of sequence identity are useful in identifying related polynucleotide sequences. Useful examples of percent identities are those listed above, or also preferred is any integer percentage from 80% to 100%, such as 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98 and 99%.

[0106] A "substantially homologous sequence" refers to variants of the disclosed sequences such as those that result from site-directed mutagenesis, as well as synthetically derived sequences. A substantially homologous sequence of the present invention also refers to those fragments of a particular promoter nucleotide sequence disclosed herein that operate to promote the inducible expression of an operably linked heterologous nucleic acid fragment. These promoter fragments will comprise at least about 20 contiguous nucleotides, preferably at least about 50 contiguous nucleotides, more preferably at least about 75 contiguous nucleotides, even more preferably at least about 100 contiguous nucleotides of the particular promoter nucleotide sequence disclosed herein. The nucleotides of such fragments will usually comprise the TATA recognition sequence of the particular promoter sequence. Such fragments may be obtained by use of restriction enzymes to cleave the naturally occurring promoter nucleotide sequences disclosed herein; by synthesizing a nucleotide sequence from the naturally occurring promoter DNA sequence; or may be obtained through the use of PCR technology. See particularly, Mullis et al., Methods Enzymol. 155:335-350 (1987), and Higuchi, R. In PCR Technology: Principles and Applications for DNA Amplifications; Erlich, H. A., Ed.; Stockton Press Inc.: New York, 1989. Again, variants of these promoter fragments, such as those resulting from site-directed mutagenesis, are encompassed by the compositions of the present invention.

[0107] "Codon degeneracy" refers to divergence in the genetic code permitting variation of the nucleotide sequence without affecting the amino acid sequence of an encoded polypeptide. Accordingly, the instant invention relates to any nucleic acid fragment comprising a nucleotide sequence that encodes all or a substantial portion of the amino acid sequences set forth herein. The skilled artisan is well aware of the "codon-bias" exhibited by a specific host cell in usage of nucleotide codons to specify a given amino acid. Therefore, when synthesizing a nucleic acid fragment for improved expression in a host cell, it is desirable to design the nucleic acid fragment such that its frequency of codon usage approaches the frequency of preferred codon usage of the host cell.

[0108] Sequence alignments and percent similarity calculations may be determined using the Megalign program of the LASARGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.) or using the AlignX program of the Vector NTI bioinformatics computing suite (Invitrogen). Multiple alignment of the sequences are performed using the Clustal method of alignment (Higgins and Sharp, CABIOS 5:151-153 (1989)) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments and calculation of percent identity of protein sequences using the Clustal method are KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5. For nucleic acids these parameters are GAP PENALTY=10, GAP LENGTH PENALTY=10, KTUPLE=2, GAP PENALTY=5. WINDOW=4 and DIAGONALS SAVED=4. A "substantial portion" of an amino acid or nucleotide sequence comprises enough of the amino acid sequence of a polypeptide or the nucleotide sequence of a gene to afford putative identification of that polypeptide or gene, either by manual evaluation of the sequence by one skilled in the art, or by computer-automated sequence comparison and identification using algorithms such as BLAST (Altschul, S. F. et al., J. Mol. Biol. 215:403-410 (1993)) and Gapped Blast (Altschul, S. F. et al., Nucleic Acids Res. 25:3389-3402 (1997)). BLASTN refers to a BLAST program that compares a nucleotide query sequence against a nucleotide sequence database.

[0109] "Gene" refers to a nucleic acid fragment that expresses a specific protein, including regulatory sequences preceding (5' non-coding sequences) and following (3' non-coding sequences) the coding sequence. "Native gene" refers to a gene as found in nature with its own regulatory sequences. "Chimeric gene" or "recombinant expression construct", which are used interchangeably, refers to any gene that is not a native gene, comprising regulatory and coding sequences that are not found together in nature. Accordingly, a chimeric gene may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. "Endogenous gene" refers to a native gene in its natural location in the genome of an organism. A "foreign" gene refers to a gene not normally found in the host organism, but that is introduced into the host organism by gene transfer. Foreign genes can comprise native genes inserted into a non-native organism, or chimeric genes. A "transgene" is a gene that has been introduced into the genome by a transformation procedure.

[0110] "Coding sequence" refers to a DNA sequence which codes for a specific amino acid sequence. "Regulatory sequences" refer to nucleotide sequences located upstream (5' non-coding sequences), within, or downstream (3' non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence. Regulatory sequences may include, but are not limited to, promoters, translation leader sequences, introns, and polyadenylation recognition sequences.

[0111] The "5' non-coding sequences" refer to DNA sequences located upstream of a coding sequence which influence the transcription, RNA processing or stability, or translation of the associated coding sequence.

[0112] An "intron" is an intervening sequence in a gene that is transcribed into RNA but is then excised in the process of generating the mature mRNA. The term is also used for the excised RNA sequences. An "exon" is a portion of the sequence of a gene that is transcribed and is found in the mature messenger RNA derived from the gene, but is not necessarily a part of the sequence that encodes the final gene product.

[0113] The term "constitutive promoter" refers to promoters active in all or most tissues of a plant at all or most developing stages. As with other promoters classified as "constitutive" (e.g. ubiquitin), some variation in absolute levels of expression can exist among different tissues or stages.

[0114] The term "constitutive promoter" or "tissue-independent" are used interchangeably herein

[0115] The term "tissue specific promoter" refers to promoters that have been shown to direct RNA synthesis at higher levels only in particular types of cells or tissues and are often referred to as "tissue specific promoters", or "tissue-preferred promoters" if the promoters direct RNA synthesis preferably in certain tissues but also in other tissues at reduced levels.

[0116] Among the most commonly used promoters are the nopaline synthase (NOS) promoter (Ebert et al., Proc. Natl. Acad. Sci. U.S.A. 84:5745-5749 (1987)), the octapine synthase (OCS) promoter, caulimovirus promoters such as the cauliflower mosaic virus (CaMV) 19S promoter (Lawton et al., Plant Mol. Biol. 9:315-324 (1987)), the CaMV 35S promoter (Odell et al., Nature 313:810-812 (1985)), and the figwort mosaic virus 35S promoter (Sanger et al., Plant Mol. Biol. 14:433-43 (1990)), the light inducible promoter from the small subunit of rubisco, the Adh promoter (Walker et al., Proc. Natl. Acad. Sci. U.S.A. 84:6624-66280 (1987), the sucrose synthase promoter (Yang et al., Proc. Natl. Acad. Sci. U.S.A. 87:4144-4148 (1990)), the R gene complex promoter (Chandler et al., Plant Cell 1:1175-1183 (1989)), the chlorophyll a/b binding protein gene promoter, etc. Other commonly used promoters are, the promoters for the potato tuber ADPGPP genes, the sucrose synthase promoter, the granule bound starch synthase promoter, the glutelin gene promoter, the maize waxy promoter, Brittle gene promoter, and Shrunken 2 promoter, the acid chitinase gene promoter, and the zein gene promoters (15 kD, 16 kD, 19 kD, 22 kD, and 27 kD; Perdersen et al., Cell 29:1015-1026 (1982)). A plethora of promoters is described in PCT Publication No. WO 00/18963 published on Apr. 6, 2000, the disclosure of which is hereby incorporated by reference.

[0117] The "translation leader sequence" refers to a DNA sequence located between the promoter sequence of a gene and the coding sequence. The translation leader sequence is present in the fully processed mRNA upstream of the translation start sequence. The translation leader sequence may affect processing of the primary transcript to mRNA, mRNA stability or translation efficiency. Examples of translation leader sequences have been described (Turner, R. and Foster, G. D., Molecular Biotechnology 3:225 (1995)).

[0118] The "3' non-coding sequences" refer to DNA sequences located downstream of a coding sequence and include polyadenylation recognition sequences and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3' end of the mRNA precursor. The use of different 3' non-coding sequences is exemplified by Ingelbrecht et al., Plant Cell 1:671-680 (1989).

[0119] "RNA transcript" refers to a product resulting from RNA polymerase-catalyzed transcription of a DNA sequence. When an RNA transcript is a perfect complementary copy of a DNA sequence, it is referred to as a primary transcript or it may be a RNA sequence derived from posttranscriptional processing of a primary transcript and is referred to as a mature RNA. "Messenger RNA" ("mRNA") refers to RNA that is without introns and that can be translated into protein by the cell. "cDNA" refers to a DNA that is complementary to and synthesized from an mRNA template using the enzyme reverse transcriptase. The cDNA can be single-stranded or converted into the double-stranded by using the Klenow fragment of DNA polymerase I. "Sense" RNA refers to RNA transcript that includes mRNA and so can be translated into protein within a cell or in vitro. "Antisense RNA" refers to a RNA transcript that is complementary to all or part of a target primary transcript or mRNA and that blocks expression or transcripts accumulation of a target gene (U.S. Pat. No. 5,107,065). The complementarity of an antisense RNA may be with any part of the specific gene transcript, i.e. at the 5' non-coding sequence, 3' non-coding sequence, introns, or the coding sequence. "Functional RNA" refers to antisense RNA, ribozyme RNA, or other RNA that may not be translated but yet has an effect on cellular processes.

[0120] The term "operably linked" refers to the association of nucleic acid sequences on a single nucleic acid fragment so that the function of one is affected by the other. For example, a promoter is operably linked with a coding sequence when it is capable of affecting the expression of that coding sequence (i.e., that the coding sequence is under the transcriptional control of the promoter). Coding sequences can be operably linked to regulatory sequences in sense or antisense orientation.

[0121] The term "expression", as used herein, refers to the production of a functional end-product e.g., an mRNA or a protein (precursor or mature).

[0122] The term "expression cassette" as used herein, refers to a discrete nucleic acid fragment into which a nucleic acid sequence or fragment can be moved.

[0123] Expression or overexpression of a gene involves transcription of the gene and translation of the mRNA into a precursor or mature protein. "Antisense inhibition" refers to the production of antisense RNA transcripts capable of suppressing the expression of the target protein. "Overexpression" refers to the production of a gene product in transgenic organisms that exceeds levels of production in normal or non-transformed organisms. "Co-suppression" refers to the production of sense RNA transcripts capable of suppressing the expression or transcript accumulation of identical or substantially similar foreign or endogenous genes (U.S. Pat. No. 5,231,020). The mechanism of co-suppression may be at the DNA level (such as DNA methylation), at the transcriptional level, or at post-transcriptional level.

[0124] Co-suppression constructs in plants previously have been designed by focusing on overexpression of a nucleic acid sequence having homology to an endogenous mRNA, in the sense orientation, which results in the reduction of all RNA having homology to the overexpressed sequence (see Vaucheret et al., Plant J. 16:651-659 (1998); and Gura, Nature 404:804-808 (2000)). The overall efficiency of this phenomenon is low, and the extent of the RNA reduction is widely variable. Recent work has described the use of "hairpin" structures that incorporate all, or part, of an mRNA encoding sequence in a complementary orientation that results in a potential "stem-loop" structure for the expressed RNA (PCT Publication No. WO 99/53050 published on Oct. 21, 1999; and PCT Publication No. WO 02/00904 published on Jan. 3, 2002). This increases the frequency of co-suppression in the recovered transgenic plants. Another variation describes the use of plant viral sequences to direct the suppression, or "silencing", of proximal mRNA encoding sequences (PCT Publication No. WO 98/36083 published on Aug. 20, 1998). Genetic and molecular evidences have been obtained suggesting that dsRNA mediated mRNA cleavage may have been the conserved mechanism underlying these gene silencing phenomena (Elmayan et al., Plant Cell 10:1747-1757 (1998); Galun, In Vitro Cell. Dev. Biol. Plant 41(2):113-123 (2005); Pickford et al, Cell. Mol. Life Sci. 60(5):871-882 (2003)).

[0125] As stated herein, "suppression" refers to a reduction of the level of enzyme activity or protein functionality (e.g., a phenotype associated with a protein) detectable in a transgenic plant when compared to the level of enzyme activity or protein functionality detectable in a non-transgenic or wild type plant with the native enzyme or protein. The level of enzyme activity in a plant with the native enzyme is referred to herein as "wild type" activity. The level of protein functionality in a plant with the native protein is referred to herein as "wild type" functionality. The term "suppression" includes lower, reduce, decline, decrease, inhibit, eliminate and prevent. This reduction may be due to a decrease in translation of the native mRNA into an active enzyme or functional protein. It may also be due to the transcription of the native DNA into decreased amounts of mRNA and/or to rapid degradation of the native mRNA. The term "native enzyme" refers to an enzyme that is produced naturally in a non-transgenic or wild type cell. The terms "non-transgenic" and "wild type" are used interchangeably herein.

[0126] "Altering expression" refers to the production of gene product(s) in transgenic organisms in amounts or proportions that differ significantly from the amount of the gene product(s) produced by the corresponding wild-type organisms (i.e., expression is increased or decreased).

[0127] "Transformation" refers to the transfer of a nucleic acid fragment into the genome of a host organism, resulting in genetically stable inheritance. Host organisms containing the transformed nucleic acid fragments are referred to as "transgenic" organisms. The preferred method of soybean cell transformation is the use of particle-accelerated or "gene gun" transformation technology (Klein, T., Nature (London) 327:70-73 (1987); U.S. Pat. No. 4,945,050).

[0128] "Transient expression" refers to the temporary expression of often reporter genes such as β-glucuronidase (GUS), fluorescent protein genes GFP, ZS-YELLOW1 N1, AM-CYAN1, DS-RED in selected certain cell types of the host organism in which the transgenic gene is introduced temporally by a transformation method. The transformed materials of the host organism are subsequently discarded after the transient gene expression assay.

[0129] Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described more fully in Sambrook, J. et al., In Molecular Cloning: A Laboratory Manual; 2^nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, N.Y., 1989 (hereinafter "Sambrook et al., 1989") or Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K., Eds.; In Current Protocols in Molecular Biology; John Wiley and Sons: New York, 1990 (hereinafter "Ausubel et al., 1990").

[0130] "PCR" or "Polymerase Chain Reaction" is a technique for the synthesis of large quantities of specific DNA segments, consisting of a series of repetitive cycles (Perkin Elmer Cetus Instruments, Norwalk, Conn.). Typically, the double stranded DNA is heat denatured, the two primers complementary to the 3' boundaries of the target segment are annealed at low temperature and then extended at an intermediate temperature. One set of these three consecutive steps comprises a cycle.

[0131] The terms "recombinant polynucleotide", "recombinant nucleotide", "recombinant DNA", "recombinant DNA construct" and "recombinant expression construct" are used interchangeably herein. A recombinant DNA construct comprises an artificial or heterologous combination of nucleic acid sequences, e.g., regulatory and coding sequences that are not found together in nature. For example, a recombinant DNA construct can comprise a plasmid vector or a fragment thereof comprising the instant inducible promoter and a heterologous polynucleotide of interest. In other embodiments, a recombinant construct may comprise regulatory sequences and coding sequences that are derived from maize, rice, sorghum or different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. Such a construct may be used by itself or may be used in conjunction with a vector. If a vector is used, then the choice of vector is dependent upon the method that will be used to transform host cells as is well known to those skilled in the art. For example, a plasmid vector can be used. The skilled artisan is well aware of the genetic elements that must be present on the vector in order to successfully transform, select and propagate host cells comprising any of the isolated nucleic acid fragments provided herein. The skilled artisan will also recognize that different independent transformation events will result in different levels and patterns of expression (Jones et al., EMBO J. 4:2411-2418 (1985); De Almeida et al., Mol. Gen. Genetics 218:78-86 (1989)), and thus that multiple events must be screened in order to obtain lines displaying the desired expression level and pattern. Such screening may be accomplished by Southern analysis of DNA, Northern analysis of mRNA expression, immunoblotting analysis of protein expression, or phenotypic analysis, among others.

[0132] It is demonstrated herein that the maize mannitol dehydrogenase gene promoter ZmCAS1 can, in fact, be used as an inducible promoter to drive efficient expression of transgenes, and that such promoter can be isolated and used by one skilled in the art. Induced GUS and MS45 expression has been observed in sink tissues such as anthers, callus, root and shoots of seedlings as well as developing leaves (Examples 1-3)`

[0133] Mannitol metabolism plays an important role in plant responses to both biotic and abiotic stresses. (Stoop et al. 2001, Trends in Plant Science, Volume 1, Issue 5, May 1996, Pages 139-144). Celery plants exposed to high salinity showed an increased mannitol accumulation primarily caused by a decrease in mannitol dehydrogenase activity in sink tissues (Stoop and Pharr. 1993 Plant Physiol. 103:1001-1008). As shown in FIG. 1 B, the ZmCAS1cDNA (SEQ ID NO:5) showed a high % identity with a maize mannitol dehydrogenase (GI:226528549; SEQ ID NO:6), FIG. 1(B). Taken together with our observations that the ZmCAS1 promoter can be induced by a chemical such as a safener, or a stress such as a heat treatment, one can further test the ability of the ZmCAS1 promoter to be responsive to stresses such as, but not limited to, drought, osmotic or salt stress, or a combination thereof.

[0134] It is clear from the disclosure set forth herein that one of ordinary skill in the art could perform the following procedure:

[0135] 1) operably linking the nucleic acid fragment containing the ZMCAS1 promoter sequence to a suitable reporter gene; there are a variety of reporter genes that are well known to those skilled in the art, including the bacterial GUS gene, the firefly luciferase gene, and the cyan, green, red, and yellow fluorescent protein genes; any gene for which an easy and reliable assay is available can serve as the reporter gene.

[0136] 2) transforming a chimeric ZmCAS1 promoter:reporter gene expression cassette into an appropriate plant for expression of the promoter. There are a variety of appropriate plants which can be used as a host for transformation that are well known to those skilled in the art, including the dicots, Arabidopsis, tobacco, soybean, oilseed rape, peanut, sunflower, safflower, cotton, tomato, potato, cocoa and the monocots, corn, wheat, rice, barley and palm.

[0137] 3) testing for expression of the ZmCAS1 promoter in various cell types of transgenic plant tissues, e.g., leaves, roots, flowers, seeds, transformed with the chimeric ZmCAS1 promoter:reporter gene expression cassette by assaying for expression of the reporter gene product.

[0138] In another aspect, this invention concerns a recombinant DNA construct comprising at least one heterologous nucleic acid fragment operably linked to any promoter, or combination of promoter elements, of the present invention. Recombinant DNA constructs can be constructed by operably linking the nucleic acid fragment of the invention promoter or a fragment that is substantially similar and functionally equivalent to any portion of the nucleotide sequence set forth in SEQ ID NOs: 9 or 10 to a heterologous nucleic acid fragment. Any heterologous nucleic acid fragment can be used to practice the invention. The selection will depend upon the desired application or phenotype to be achieved. The various nucleic acid sequences can be manipulated so as to provide for the nucleic acid sequences in the proper orientation. It is believed that various combinations of promoter elements as described herein may be useful in practicing the present invention.

[0139] In another aspect, this invention concerns a recombinant DNA construct comprising at least one acetolactate synthase (ALS) nucleic acid fragment operably linked to ZmCAS1 promoter, or combination of promoter elements, of the present invention. The acetolactate synthase gene is involved in the biosynthesis of branched chain amino acids in plants and is the site of action of several herbicides including sulfonyl urea. Expression of a mutated acetolactate synthase gene encoding a protein that can no longer bind the herbicide will enable the transgenic plants to be resistant to the herbicide (U.S. Pat. No. 5,605,011, U.S. Pat. No. 5,378,824). The mutated acetolactate synthase gene is also widely used in plant transformation to select transgenic plants.

[0140] In another embodiment, this invention concerns host cells comprising either the recombinant DNA constructs of the invention as described herein or isolated polynucleotides of the invention as described herein. Examples of host cells which can be used to practice the invention include, but are not limited to, yeast, bacteria, and plants.

[0141] Plasmid vectors comprising the instant recombinant expression construct can be constructed. The choice of plasmid vector is dependent upon the method that will be used to transform host cells. The skilled artisan is well aware of the genetic elements that must be present on the plasmid vector in order to successfully transform, select and propagate host cells containing the chimeric gene.

[0142] The method of transformation/transfection is not critical to the instant invention; various methods of transformation or transfection are currently available. As newer methods are available to transform crops or other host cells they may be directly applied. Accordingly, a wide variety of methods have been developed to insert a DNA sequence into the genome of a host cell to obtain the transcription or transcript and translation of the sequence to effect phenotypic changes in the organism. Thus, any method which provides for efficient transformation/transfection may be employed.

[0143] Methods for introducing expression vectors into plant tissue available to one skilled in the art are varied and will depend on the plant selected. Procedures for transforming a wide variety of plant species are well known and described throughout the literature. See, for example, Miki et al, "Procedures for Introducing Foreign DNA into Plants" in Methods in Plant Molecular Biotechnology, supra; Klein et al, Bio/Technology 10:268 (1992); and Weising et al., Ann. Rev. Genet. 22: 421-477 (1988). For example, the DNA construct may be introduced into the genomic DNA of the plant cell using techniques such as microprojectile-mediated delivery, Klein et al., Nature 327: 70-73 (1987); electroporation, Fromm et al., Proc. Natl. Acad. Sci. 82: 5824 (1985); polyethylene glycol (PEG) precipitation, Paszkowski et al., EMBO J. 3: 2717-2722 (1984); direct gene transfer WO 85/01856 and EP No. 0 275 069; in vitro protoplast transformation, U.S. Pat. No. 4,684,611; and microinjection of plant cell protoplasts or embryogenic callus, Crossway, Mol. Gen. Genetics 202:179-185 (1985). Co-cultivation of plant tissue with Agrobacterium tumefaciens is another option, where the DNA constructs are placed into a binary vector system. See e.g., U.S. Pat. No. 5,591,616; Ishida et al., "High Efficiency Transformation of Maize (Zea mays L.) mediated by Agrobacterium tumefaciens" Nature Biotechnology 14:745-750 (1996). The virulence functions of the Agrobacterium tumefaciens host will direct the insertion of the construct into the plant cell DNA when the cell is infected by the bacteria. See, for example Horsch et al., Science 233: 496-498 (1984), and Fraley et al., Proc. Natl. Acad. Sci. 80: 4803 (1983).

[0144] Standard methods for transformation of canola are described at Moloney et al. "High Efficiency Transformation of Brassica napus using Agrobacterium Vectors" Plant Cell Reports 8:238-242 (1989). Corn transformation is described by Fromm et al, Bio/Technology 8:833 (1990). Agrobacterium is primarily used in dicots, but certain monocots such as maize can be transformed by Agrobacterium (U.S. Pat. No. 5,550,318). Rice transformation is described by Hiei et al., "Efficient Transformation of Rice (Oryza sativa L.) Mediated by Agrobacterium and Sequence Analysis of the Boundaries of the T-DNA" The Plant Joumal 6(2): 271-282 (1994, Christou et al, Trends in Biotechnology 10:239 (1992) and Lee et al, Proc. Nat'l Acad. Sci. USA 88:6389 (1991). Wheat can be transformed by techniques similar to those used for transforming com or rice. Sorghum transformation is described at Casas et al, supra and sorghum by Wan et al, PlantPhysiol. 104:37 (1994). Soybean transformation is described in a number of publications, including U.S. Pat. No. 5,015,580.

[0145] When referring to "introduction" of the nucleotide sequence into a plant, it is meant that this can occur by direct transformation methods, such as Agrobacterium transformation of plant tissue, microprojectile bombardment, electroporation, or any one of many methods known to one skilled in the art; or, it can occur by crossing a plant having the heterologous nucleotide sequence with another plant so that progeny have the nucleotide sequence incorporated into their genomes. Such breeding techniques are well known to one skilled in the art.

[0146] Methods for transforming dicots, primarily by use of Agrobacterium tumefaciens, and obtaining transgenic plants have been published, among others, for cotton (U.S. Pat. No. 5,004,863, U.S. Pat. No. 5,159,135); soybean (U.S. Pat. No. 5,569,834, U.S. Pat. No. 5,416,011); Brassica (U.S. Pat. No. 5,463,174); peanut (Cheng et al., Plant Cell Rep. 15:653-657 (1996), McKently et al., Plant Cell Rep. 14:699-703 (1995)); papaya (Ling et al., Bio/technology 9:752-758 (1991)); and pea (Grant et al., Plant Cell Rep. 15:254-258 (1995)). For a review of other commonly used methods of plant transformation see Newell, C. A., Mol. Biotechnol. 16:53-65 (2000). One of these methods of transformation uses Agrobacterium rhizogenes (Tepfler, M. and Casse-Delbart, F., Microbiol. Sci. 4:24-28 (1987)). Transformation of soybeans using direct delivery of DNA has been published using PEG fusion (PCT Publication No. WO 92/17598), electroporation (Chowrira et al., Mol. Biotechnol. 3:17-23 (1995); Christou et al., Proc. Natl. Acad. Sci. U.S.A. 84:3962-3966 (1987)), microinjection, or particle bombardment (McCabe et al., BiolTechnology 6:923 (1988); Christou et al., Plant Physiol. 87:671-674 (1988)).

[0147] There are a variety of methods for the regeneration of plants from plant tissues. The particular method of regeneration will depend on the starting plant tissue and the particular plant species to be regenerated. The regeneration, development and cultivation of plants from single plant protoplast transformants or from various transformed explants is well known in the art (Weissbach and Weissbach, Eds.; In Methods for Plant Molecular Biology; Academic Press, Inc.: San Diego, Calif., 1988). This regeneration and growth process typically includes the steps of selection of transformed cells, culturing those individualized cells through the usual stages of embryonic development or through the rooted plantlet stage. Transgenic embryos and seeds are similarly regenerated. The resulting transgenic rooted shoots are thereafter planted in an appropriate plant growth medium such as soil. Preferably, the regenerated plants are self-pollinated to provide homozygous transgenic plants. Otherwise, pollen obtained from the regenerated plants is crossed to seed-grown plants of agronomically important lines. Conversely, pollen from plants of these important lines is used to pollinate regenerated plants. A transgenic plant of the present invention containing a desired polypeptide is cultivated using methods well known to one skilled in the art.

[0148] In addition to the above discussed procedures, practitioners are familiar with the standard resource materials which describe specific conditions and procedures for the construction, manipulation and isolation of macromolecules (e.g., DNA molecules, plasmids, etc.), generation of recombinant DNA fragments and recombinant expression constructs and the screening and isolating of clones, (see for example, Sambrook, J. et al., In Molecular Cloning: A Laboratory Manual; 2^nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, N.Y., 1989; Maliga et al., In Methods in Plant Molecular Biology; Cold Spring Harbor Press, 1995; Birren et al., In Genome Analysis: Detecting Genes, 1; Cold Spring Harbor N.Y., 1998; Birren et al., In Genome Analysis: Analyzing DNA, 2; Cold Spring Harbor: New York, 1998; Clark, Ed., In Plant Molecular Biology: A Laboratory Manual; Springer: New York, 1997).

[0149] The skilled artisan will also recognize that different independent transformation events will result in different levels and patterns of expression of the chimeric genes (Jones et al., EMBO J. 4:2411-2418 (1985); De Almeida et al., Mol. Gen. Genetics 218:78-86 (1989)). Thus, multiple events must be screened in order to obtain lines displaying the desired expression level and pattern. Such screening may be accomplished by Northern analysis of mRNA expression, Western analysis of protein expression, or phenotypic analysis. Also of interest are seeds obtained from transformed plants displaying the desired gene expression profile.

[0150] Inducible expression of chimeric genes in most plant cells makes the ZmCAS1 promoter of the instant invention especially useful when inducible expression of a target heterologous nucleic acid fragment is required.

[0151] Another general application of the ZmCAS1 promoter of the invention is to construct chimeric genes that can be used to reduce expression of at least one heterologous nucleic acid fragment in a plant cell. To accomplish this, a chimeric gene designed for gene silencing of a heterologous nucleic acid fragment can be constructed by linking the fragment to the ZmCAS1 promoter of the present invention. (See U.S. Pat. No. 5,231,020, and PCT Publication No. WO 99/53050 published on Oct. 21, 1999, PCT Publication No. WO 02/00904 published on Jan. 3, 2002, and PCT Publication No. WO 98/36083 published on Aug. 20, 1998, for methodology to block plant gene expression via cosuppression.) Alternatively, a chimeric gene designed to express antisense RNA for a heterologous nucleic acid fragment can be constructed by linking the fragment in reverse orientation to the ZmCAS1 promoter of the present invention. (See U.S. Pat. No. 5,107,065 for methodology to block plant gene expression via antisense RNA.) Either the cosuppression or antisense chimeric gene can be introduced into plants via transformation. Transformants wherein expression of the heterologous nucleic acid fragment is decreased or eliminated are then selected.

[0152] This invention also concerns a method of altering (increasing or decreasing) the expression of at least one heterologous nucleic acid fragment in a plant cell which comprises:

[0153] (a) transforming a plant cell with the recombinant expression construct of described herein;

[0154] (b) induction of the inducible promoter by chemical or stress treatment on the cell of (a)

[0155] (c) growing fertile mature plants from the transformed plant cell of step (a); and,

[0156] (d) selecting plants containing the transformed plant cell wherein the expression of the heterologous nucleic acid fragment is increased or decreased. Transformation and selection can be accomplished using methods well-known to those skilled in the art including, but not limited to, the methods described herein.

[0157] Non-limiting examples of compositions and methods disclosed herein are as follows:

[0158] 1. An isolated polynucleotide comprising:

[0159] a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:9 or SEQ ID NO:10, or a full-length complement thereof;

[0160] b) a nucleotide sequence comprising a functional fragment of SEQ ID NO:10, or a full-length complement thereof;

[0161] c) a nucleotide sequence comprising a sequence having at least 85% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a) or (b);

[0162] d) a nucleotide sequence which hybridizes to SEQ ID NO:9 under highly stringent conditions of a wash of 0.1 SSC, 0.1% (w/v) SDS at 65° C.;

[0163] e) a nucleotide sequence comprising all or a fragment of a 1.7 kb 5' non-coding sequence of a mannitol dehydrogenase; or,

[0164] f) a derivative of one of the nucleotide sequences indicated in (a), (b), (c), (d) or (e) obtained by substitution, addition and/or deletion of one or more nucleotides; and,

[0165] wherein said nucleotide sequence is an inducible promoter.

[0166] 2. The isolated polynucleotide of embodiment 1, wherein the nucleotide sequence of c) has at least 90% identity, based on the BLASTN method of alignment, when compared to the sequence set forth in SEQ ID NO:1.

[0167] 3. The isolated polynucleotide of embodiment 1, wherein the nucleotide sequence of c) has at least 95% identity, based on the BLASTN method of alignment, when compared to the sequence set forth in SEQ ID NO:1.

[0168] 4. The isolated polynucleotide of embodiment 1, wherein the nucleotide sequence of c) has at least 98% identity, based on the BLASTN method of alignment, when compared to the sequence set forth in SEQ ID NO:1.

[0169] 5. The isolated polynucleotide of embodiment 1 wherein said inducible promoter is induced by a chemical or stress treatment.

[0170] 6. The isolated polynucleotide of embodiment 1 wherein said inducible promoter is induced by a safener or heat treatment.

[0171] 7. The isolated polynucleotide of embodiment 6, wherein the safener is N-(aminocarbonyl)-2-chlorobenzenesulfonamide.

[0172] 8. The isolated polynucleotide of embodiment 6, wherein said heat treatment comprises a temperature greater than 26° C.

[0173] 9. A recombinant DNA construct comprising the isolated polynucleotide of embodiment 1 operably linked to at least one heterologous nucleic acid sequence.

[0174] 10. The recombinant DNA construct of embodiment 9, wherein the heterologous nucleic acid sequence codes for a gene selected from the group consisting of: a double-strand break inducing gene, a recombinase gene, a reporter gene, a selection marker, a disease resistance conferring gene, a herbicide resistance conferring gene, an insect resistance conferring gene; a gene involved in carbohydrate metabolism, a gene involved in fatty acid metabolism, a gene involved in amino acid metabolism, a gene involved in plant development, a gene involved in plant growth regulation, a gene involved in yield improvement, a gene involved in drought resistance, a gene involved in cold resistance, a gene involved in heat and salt resistance in plants.

[0175] 11. The recombinant DNA construct of embodiment 9, wherein the heterologous nucleic acid sequence encodes a protein selected from the group consisting of: a double-strand break inducing protein, a recombinase protein, a reporter protein, a selection marker, a protein conferring disease resistance, protein conferring herbicide resistance, protein conferring insect resistance; protein involved in carbohydrate metabolism, protein involved in fatty acid metabolism, protein involved in amino acid metabolism, protein involved in plant development, protein involved in plant growth regulation, protein involved in yield improvement, protein involved in drought resistance, protein involved in cold resistance, protein involved in heat resistance and salt resistance in plants.

[0176] 12. A vector comprising the recombinant DNA construct of embodiment 9.

[0177] 13. A cell comprising the recombinant DNA construct of embodiment 9.

[0178] 14. The cell of embodiment 13, wherein the cell is a plant cell.

[0179] 15. The plant cell of embodiment 14 having stably incorporated into its genome the recombinant DNA construct of embodiment 9.

[0180] 16. A transgenic plant having stably incorporated into its genome the recombinant DNA construct of embodiment 9.

[0181] 17. The transgenic plant of embodiment 16 wherein said plant is a monocot plant.

[0182] 18. The transgenic plant of embodiment 17, wherein said monocot is selected from the group comprising: maize, wheat, rice, barley, sorghum, millet, sugarcane and rye.

[0183] 19. The transgenic plant of embodiment 16, wherein said plant is a dicot plant.

[0184] 20. The transgenic plant of embodiment 19, wherein said dicot is selected from the group comprising: soy, Brassica sp., cotton, safflower, tobacco, alfalfa and sunflower.

[0185] 21. Transgenic seed produced by the transgenic plant of embodiment 16.

[0186] 22. A plant stably transformed with a recombinant expression construct comprising a plant promoter and a heterologous nucleic acid fragment operably linked to said promoter, wherein said promoter is an inducible promoter and capable of controlling expression of said heterologous nucleic acid fragment in a plant cell, and further wherein said promoter comprises a fragment of SEQ ID NO:10.

[0187] 23. A method of expressing a coding sequence or a functional RNA in a plant cell comprising:

[0188] a) introducing the recombinant DNA construct of embodiment 9 into a plant cell, wherein the at least one heterologous sequence comprises a coding sequence or a functional RNA;

[0189] b) growing the plant cell of step a);

[0190] c) induction of the inducible promoter by chemical or stress treatment on the plant cell of b); and,

[0191] d) selecting a plant cell displaying expression of the coding sequence or the functional RNA of the recombinant DNA construct.

[0192] 24. The method of embodiment 23, wherein the chemical is a safener.

[0193] 25. The method of embodiment 23 wherein the stress treatment is a heat treatment.

[0194] 26. The method of embodiment 23 further comprising growing the plant cell of d) into a plant.

[0195] 27. A method of expressing a coding sequence or a functional RNA in anther cells, said method comprising:

[0196] a) introducing the recombinant DNA construct of embodiment 9 into a plant cell, wherein the at least one heterologous sequence comprises a coding sequence or a functional RNA;

[0197] b) growing the plant cell of step a);

[0198] c) induction of the inducible promoter by chemical or stress treatment on the plant cell of b); and,

[0199] d) identification of anther cells displaying expression of the coding sequence or the functional RNA of the recombinant DNA construct.

[0200] 28. The method of embodiment 23 or embodiment 27 wherein the at least one heterologous sequence is transiently expressed.

[0201] 29. The method of embodiment 23 or embodiment 27 wherein the at least one heterologous sequence is stably incorporated in the plant cell.

[0202] 30. A method for altering expression of at least one heterologous nucleic acid fragment in a plant comprising:

[0203] (a) transforming a plant cell with the recombinant expression construct of embodiment 9;

[0204] (b) induction of the inducible promoter by chemical or stress treatment on the cell of (a)

[0205] (c) growing fertile mature plants from the transformed plant cell of step (a); and,

[0206] (d) selecting plants containing the transformed plant cell wherein the expression of the heterologous nucleic acid fragment is increased or decreased.

[0207] 31. A method of transgenically altering a marketable plant trait, comprising:

[0208] a) introducing a recombinant DNA construct of embodiment 9 into a plant;

[0209] b) induction of the inducible promoter by chemical or stress treatment on the plant of (a);

[0210] c) growing a fertile, mature plant resulting from step b); and

[0211] d) selecting a plant expressing the at least one heterologous nucleotide sequence in at least one plant tissue based on the altered marketable trait.

[0212] 32. The method of embodiment 31 wherein the marketable trait is selected from the group consisting of: disease resistance, herbicide resistance, insect resistance carbohydrate metabolism, fatty acid metabolism, amino acid metabolism, plant development, plant growth regulation, yield improvement, drought resistance, cold resistance, heat resistance, and salt resistance.

[0213] 33. An isolated polynucleotide comprising:

[0214] a) a nucleotide sequence comprising all or a functional fragment of SEQ ID NO:19 or SEQ ID NO:22;

[0215] b) a nucleotide sequence comprising a full-length complement of the nucleotide sequence (a); or,

[0216] c) a nucleotide sequence comprising a sequence having at least 90% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a) or (b); and, wherein said nucleotide sequence is a promoter.

[0217] 34. The isolate polynucleotide of embodiment 33 wherein said promoter is an inducible promoter.

EXAMPLES

[0218] The present invention is further defined in the following Examples, in which parts and percentages are by weight and degrees are Celsius, unless otherwise stated. Sequences of promoters, cDNA, adaptors, and primers listed in this invention all are in the 5' to 3' orientation unless described otherwise. Techniques in molecular biology were typically performed as described in Ausubel, F. M. et al., In Current Protocols in Molecular Biology; John Wiley and Sons: New York, 1990 or Sambrook, J. et al., In Molecular Cloning: A Laboratory Manual; 2^nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, N.Y., 1989 (hereinafter "Sambrook et al., 1989"). It should be understood that these Examples, while indicating preferred embodiments of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Thus, various modifications of the invention in addition to those shown and described herein will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims.

[0219] The disclosure of each reference set forth herein is incorporated herein by reference in its entirety.

Example 1

Identification of Safener-Inducible cDNAs Expressed in Microspores and/or Tapetum

[0220] Strategy Design for the Identification of Safener-Inducible cDNAs.

[0221] The isolation of conditionally regulated promoters with tissue specificity in plants which are different than the safener induced promoter ZmlN2-2 (Hershey et al. U.S. Pat. No. 5,364,780 Nov. 15, 1994) would enable conditional regulation of genes in microspores and/or the tapetum. Previously, it has been demonstrated that while ZmlN2-2 transcript expression increases in callus, leaf and anther tissues in maize after safener treatment, genes regulated by this promoter do not express in maize tapetal cells (Cigan et al. 2001. Sex. Plant Reprod. 14, 135-142). Immunolocalization studies demonstrated that genes regulated by ZmlN2-2 are present in all anther cell types except the tapetum or microspores. To date, no promoters that respond to CBSU (Chlorobenzenesulfonamide) safener and are specifically expressed in tapetal cells or microspores at the tetrad stage of microsporogenesis have been identified. To enable the isolation of safener-inducible candidate promoters that are expressed in microspores or tapetum, a strategy was designed which takes advantage of two fundamental observations made of plants transformed with the E. coli DAMethylase gene expressed from the maize anther-specific promoter 5126 (5126:DAM; Unger et al., 2001, Transgenic Res. 10, 409-422). First, cytological examination of tetrad staged anthers from male-sterile plants expressing 5126:DAM revealed abnormal microspores and nearly ablated tapetal cells in otherwise structurally normal appearing anthers. Second, Northern analysis of mRNA isolated from 5126:DAM sterile anthers indicates a loss of two tapetal-specific transcripts, 5126 and MS45, while a transcript not expected to be the tapetal-specific (maize actin), is easily detected (Cigan et al. 2001. Sex. Plant Reprod. 14, 135-142). Therefore, anthers isolated from 5126:DAM sterile plants should be reduced or perhaps completely devoid of tapetal- and/or microspore-specific mRNAs.

[0222] In addition, comparison of the ZmlN2-2 transcript expression from RNAs isolated from wild-type male-fertile CBSU-treated plants to RNAs isolated from male-sterile CBSU-treated 5126:DAM plants showed, that in contrast the MS45 and 5126 tapetal-specific mRNAs, the ZmlN2-2 was not reduced in anther RNAs isolated from 5126:DAM CBSU-treated plants (Cigan et al. 2001. Sex. Plant Reprod. 14, 135-142).

[0223] A strategy was designed using sterile plants which were reduced or devoid of tapetal- and/or microspore-specific mRNAs. The strategy involved treating maize plants with CBSU and comparing anther mRNA transcript profiles from these treated control plants with treated 5126:DAM plants. Such a strategy did lead to the identification and isolation of mRNAs and, ultimately, promoters which are responsive to the safener and are microspore- or tapetum-expressed as described below.

[0224] Toward this end, differential RNA hybridization was used to enrich for maize anther or callus mRNAs that are increased by safener or heat treatment. Subsequently, these mRNAs were used as probes to isolate cDNAs from anther cDNA libraries prepared from CBSU-treated maize plants. These cDNAs were then used to screen mRNAs isolated from male-fertile and male-sterile 5126:DAM control and safener-treated plants as a means to identify transcripts which are induced by CBSU or heat treatment or and expressed in the tapetum or microspores as described below.

Maize Anther cDNA Library Construction from CBSU-Treated Wild-Type Plants and Isolation of Safener Inducible cDNA's.

[0225] Wild type maize plants were grown to the meiosis stage of microspore development. Plants were watered with 30 mg 2-CBSU and allowed to develop to the quartet and early vacuolate stage of microspore development. PolyA+ anther RNA was isolated from wild type control and CBSU treated plants and stored. A cDNA library was constructed from mRNAs isolated from CBSU-treated plants, arrayed onto nylon filters and stored. A cDNA subtraction library was generated using the Clontech PCR-Select cDNA Subtraction Kit (#K1804-1) following the manufacturer's instructions to enrich for CBSU-specific transcripts. Using this approach anther PolyA+mRNA from wild-type plants was used to enrich for transcripts found in the anther PolyA+mRNA from CBSU treated plants. This cDNA subtraction library was subcloned into pSPORT (BRL) vector, colonies plated, picked and sequenced. Among the vector inserts sequenced, two DNA sequences were present at a high proportion, at more than 10% of the inserts sequenced, and referred to as ZmCAS1c-1 (477 bp; SEQ ID NO: 1) and ZmCAS1c-2 (438 bp; SEQ ID NO: 2) (QBSU-Anther-Subtract 1). Both ZmCAS1c-1 and ZmCAS1c-2 had sequence identity to mannitol dehydrogenases from plants. ZmCASlc-1 and ZmCAS1c-2 DNA fragments were used as hybridization probes to screen the filter arrayed cDNA CBSU-anther library described above to isolate full-length cDNA containing hybridizing clones. Both ZmCAS1c-1 and ZmCAS1c-2 identified identical cDNA clones. One cDNA clone contained a 1354 bp SalI-NotI insert (SEQ ID NO: 3) that was sequenced and identified as a 1338 bp full length cDNA clone referred to ZmCAS1cDNA (SEQ ID NO: 4). ZmCAS1cDNA is capable of encoding a 354 amino acid sequence (SEQ ID NO: 5) with 99.7% identity to a maize mannitol dehydogenase (GI number 226528549, NP_--001147757.1, SEQ ID NO:6; FIG. 1).

[0226] To determine whether the ZmCAS1 cDNA was 1) induced in maize anthers by CBSU-treatment and 2) reduced or absent in tapetum and microspore-ablated maize anthers from CBSU-treated 5126:DAM plants, a 477 bp ZmCAS1c-1 DNA fragment (SEQ ID NO:1), as well as DNA fragments from ZmMS45, Zm5126, ZmActin, and ZmUbiquitin (Cigan et al. 2001. Sex. Plant Reprod. 14, 135-142) were used as a hybridization probes against maize anther mRNAs isolated from male-fertile (F) and male-sterile (S), control (-) or CBSU-treated (+) plants. As shown in FIG. 2, the constitutively expressed maize actin (ACTIN) and ubiquitin (UBI) transcripts were easily detected and did not change their steady-state level across all anther RNA samples and treatments. MS45 and 5126 transcripts were easily detected in anther RNAs from male-fertile plants but absent in anther RNAs from male-sterile plants (FIG. 2) further supporting the observation that these RNAs are localized to the maize tapetum (Cigan et al. 2001. Sex. Plant Reprod. 14, 135-142).

[0227] Anther RNAs from control male-fertile (-, F) and control sterile plants (-, S) did not reveal detectable levels of the IN2-2 transcript, while strong hybridization signals were detected in mRNAs from anthers derived from CBSU-treated control male-fertile and male-sterile plants (FIG. 2). In contrast strong hybridization signals for ZmCAS1 are only revealed in mRNAs from anthers derived from male-fertile CBSU-treated plants (+, F). The reduced ZmCAS1 signal observed in mRNAs from anthers of male-sterile CBSU-treated plants (+, S) indicates that this ZmCAS1 transcript was present in cell layers of anthers. This observation indicates that ZmCAS1 tissue specific expression is different from the IN2-2 expression and thus makes the ZmCAS1 promoter a candidate which differs from the IN2-2 5 promoter in spatial expression in anthers.

[0228] When the ZmCAS1 probe was used to hybridize to maize callus or maize leaf treated with CBSU, strong hybridization was observed in mRNAs from callus, leaf and anther (FIG. 3), similar to the IN2-2 probe, suggesting that in addition to expression in anthers, ZmCAS1 transcript is also expressed in callus and leaf in response to safener, CBSU, treatment.

Isolation of the 1.7 Kb and Truncated 1.0 Kb ZmCAS1 Promoter Fraaments

[0229] In order to isolate DNA sequences which correspond to the ZmCAS1 promoter, subgenomic SaullIA genomic phage libraries from the maize line B73 were screened using the 477 bp ZmCAS1c-1 DNA fragment (SEQ ID NO:1) as a hybridization probe. A phage which contained a 4069 bp maize B73 DNA fragment (SEQ ID NO: 7). and hybridized to the ZmCAS1c-1 probe was isolated, plasmid excised and sequenced. DNA sequence analysis of this 4069 bp genomic DNA identified several regions of sequence identity to the ZmCAS1C cDNA. For promoter studies, oligonucleotide directed mutagenesis was used to introduce an RcaI DNA restriction site at nucleotide positions 3447-3452 in SEQ ID NO:7 using the MORPH Site-Specific Plasmid DNA Mutagenesis Kit 5 Prime-3 Prime (Boulder, Colo.) according to the vendors instructions using the oligonucleotide 5'-GCAGTTCATTCCTCATGACTGCTGCAGCAGAGC-3'(SEQ ID NO:8). A HindIII-RcaI fragment (ZmCAS1HindIII Pro, SEQ ID NO: 15) comprising the truncated 1.0 kb maize ZmCAS1 promoter of SEQ ID NO:9 and a BamHI-RcaI (ZmCAS1BamPro, SEQ ID NO: 16) fragment comprising the 1.7 kb maize ZmCAS1 promoter of SEQ ID NO:10 were isolated and used for promoter studies in plants.

[0230] As shown in FIG. 4, Example 2, and other examples described herein, both the 1.7 kb ZmCAS1 promoter (SEQ ID NO:10) and the truncated 1.0 kb ZmCAS1 promoter (SEQ ID NO:9) were active in plant cells and induced by both a safener (CBSU, FIG. 4A, 4B) and/or a heat treatment (FIG. 4B).

Example 2

Increased Expression of GUS and ZmMS45 is Observed in Maize Cells and Plants when these Aenes are Placed Under the Transcriptional Control of the ZmCAS1 Promoter in Response to the Safener. CBSU or a Heat Treatment

[0231] Agrobacterium-mediated transformation of immature embryos was used to generate integrated copies of PHP16974 (SEQ ID NO:11; ZmCAS1HindIII Pro:GUS/35SPAT) comprising the 1.0 kb ZmCAS1 promoter (SEQ ID NO:9), PHP16975 (SEQ ID NO:12, ZmCAS1BamPro:GUS/35SPAT) comprising the 1.7 kb ZmCAS1 promoter (SEQ ID NO:10) and PHP16972 (SEQ ID NO:13, ZmCAS1 HindIII Pro:MS45/35SPAT) comprising the 1.0 kb ZmCAS1 promoter or PHP16973 (SEQ ID NO:14, ZmCASIBamPro:MS45/35SPAT:) comprising the 1.7 kb ZmCAS1 promoter.

[0232] As described in Example 1 and FIG. 3, in addition to expression in anthers, ZmCAS1 transcript was also expressed in callus and leaf tissue in response to safener, CBSU, treatment. Bialophos-resistant callus events were selected for analysis and plant regeneration.

[0233] To determine whether the 1.7 kb or the 1.0 kb ZmCAS1 promoter from maize could direct induced expression of the GUS reporter, three bialophos-resistant callus events were placed onto maintenance media and maintenance media containing increasing amounts of the safener CBSU-2 for at room temperature for 18 hours, removed and stained with X-Gluc to detect GUS activity. As shown in FIG. 4A, slight GUS expression is detected in callus grown on maintenance media (FIG. 4A: C). In contrast, low levels of GUS expression are detected in callus grown on 10 mg/I CBSU (FIG. 4A: 10) while strong GUS expression is observed in PHP16975 callus events grown on 100 mg/I CBSU (FIG. 4A: 100). This data indicated that the 1.7 kb ZmCAS1 promoter is active in maize callus and can be induced by safener treatment.

[0234] Seven random bialophos-resistant callus events containing PHP16974 which contains a truncated 1.0 kb fragment of the ZmCAS1 promoter driving the GUS reporter were capable of inducible GUS expression when incubated in the presence of 100 mg/liter CBSU at room temperature (FIG. 4B; 26C+CBSU). In a separate experiment, when these 7 callus events were grown on maintenance media without CBSU but incubated for 2 days at 37° C. returned to room temperature and then stained with X-gluc, increased GUS expression was also observed (FIG. 4B; 37C). This data indicated that the truncated 1.0 kb ZmCAS1 promoter is active in maize callus and can be induced by safener and/or heat treatment.

[0235] Plants were also regenerated from callus events containing PHP16975 and grown in the greenhouse to approximately the 5 leaf stage. At this stage of development, leaf punches from plants regenerated from the 3 bialophos-resistant events shown in FIG. 5 were collected pre- (C) and post-watering (S) with 30 mg of 2-CBSU to examine GUS expression in leaf in response to application of the safener. As shown in FIG. 5 strong GUS expression was detected in leaf punches 2 days after watering (S) with CBSU across the 3 PHP16975 transformed plants analyzed.). This data further indicates that the 1.7 kb ZmCAS1 promoter is active in maize leaves and can be induced by safener treatment.

[0236] T-DNA vectors PHP16972 (SEQ ID NO:13, ZmCAS1HindIII Pro: MS45/35S:PAT) and PHP16973 (SEQ ID NO: 14, ZmCAS1 BamPro:MS45/35S:PAT) were used to transform maize callus which was generated to contain a segregating population of MS45/ms45 heterozygous and ms45/ms45 homozygous mutant plants. In order to detect MS45 RNA or protein expression under the control of the 1.0 or 1.7 kb ZmCAS promoter in maize anthers, plants containing a naturally occurring mutation in the maize MS45 gene which results in loss of MS45 RNA and protein were used for these studies. Pollen from MS45/ms45 plants were used to fertilize male-sterile ms45/ms45 plants for the purpose of generating embryos which would be ms45/ms45 as described in Cigan et al 2001. By placing the maize MS45 gene under the control of the ZmCAS1 promoter in these transformation vectors, genes other than GUS could be tested for transcriptional-induction in response to safener in callus, leaf and maize anthers as has been previously demonstrated for Zmln2-2:MS45 regulated expression (Cigan et al 2001. Sex. Plant Reprod. 14, 135-142). Five random bialophos-resistant callus events containing integrated copies of PHP16972 were placed onto maintenance media (FIG. 5, (-)) and maintenance media containing 100 mg/liter safener CBSU-2 (FIG. 5 (+)) at room temperature for 18 hours, removed and PolyA+RNA prepared and used for RNA analysis as described (Cigan et al. 2001. Sex. Plant Reprod. 14, 135-142) using the ZmMS45 and ZmActin probes for hybridization analysis. As shown in FIG. 6, strong induction of a hybridization signal corresponding to the MS45 mRNA is detected within RNA transcripts from ms45/ms45 callus grown on CBSU (+). A very low signal was observed when callus was grown in the absence of CBSU. Actin was used as a control probe to show nearly equivalent RNA levels were present in all samples. Multiple plants were regenerated from ms45/ms45 callus events transformed with PHP16973 and grown in the greenhouse. Plants were watered with 30 mg of CBSU at the meiosis stage of microspore development. Leaf and anthers (quartet, early uninucleate microspore stage) were collected 2 days later from control and CBSU-treated plants and whole-cell protein extracts were prepared from 4 leaf punches or 6 anthers as described (Cigan et al 2001). Leaf and anther proteins were electrophoresed on 10% SDS-denaturing polyacrylamide gels, transferred to supported nitrocellulose, and used for Western analysis using antibodies directed against the maize MS45 protein. Examination of leaf extracts from PHP16973 control (C) and treated (+) plants (FIG. 7) demonstrates increased steady-state levels of the MS45 protein in leaf extracts derived from CBSU-treated plants (lanes 3, 5, 7, 9). Increased MS45 protein is also detected in anther extracts (FIG. 8) derived from PHP16973 CBSU treated plants (Lane 2, 4, 7). This data further supports that the ZmCAS1 promoter is active in maize cells such as anthem, callus and leaves when induced by a safener.

[0237] Taken together the GUS and MS45 results described herein support that genes can be transcriptionally-induced when placed under the control of either the 1.7 kb or the 1.0 Kb ZmCAS1 promoter in maize cells and plants and transcription can be increased in callus, leaf and anthem in response to application of the safener CBSU and or heat treatment.

Example 3

Heat Treatment of Rice Plants Transformed with PHP16974 Comprising the Truncated 1.0 Kb ZmCAS1 Promoter Driving GUS Expression Results in GUS Expression in Germinating Seedlings

[0238] To determine whether the ZmCAS1 promoter could conditionally regulate expression in response to safener treatment or heat treatment in plant species other than maize, Agrobacterium-mediated transformation was used to generate integrated copies of PHP16974 (ZmCAS1HindIII Pro:GUS) comprising the truncated 1.0 kb ZmCAS1 promoter for studies in rice. Scutellum from 10-14 day old germinating seeds (Oryza sativa cv.Kitaake) was used for rice transformation experiment (Toki. 1997, PI Mol. Biol Reporter 15:16-21). Bialophos-resistant callus events containing PHP16974 were selected and screened for their ability to respond to safener application. Four independent bialophos-resistant events were grown on maintenance media or maintenance media containing 100 mg/liter CBSU for 24 hours, removed and stained with X-Gluc. As shown FIG. 9, strong GUS expression is observed in PHP16974 callus events when grown on media containing the CBSU safener (FIG. 9). PHP16974 bialophos-resistant events were regenerated into plants. Leaf tissue was collected from these plants and used for DNA hybridization analyses to identify single-copy PHP16974 insertions. These plants were allowed to set selfed seed and were used for subsequent studies to monitor GUS expression under the transcriptional control of the ZmCAS1 promoter. Sixteen seed were selected from 2 single-copy PHP16974 events were sterilized, grown on hormone-free media at 28° C. or 37° C. for 48 hours and allowed to germinate. Germinating seed was then incubated at 28° C. for 2 additional days and histochemically stained with X-Gluc to detect GUS activity (Reference). As shown in FIGS. 10A and 10C, seedlings germinated at 28° C. exhibit very low levels of detectable Gus staining. In contrast, rice seedlings germinated at 37° C. show pronounced blue staining and root and at the base of shoots (FIGS. 10B and 10D). These results are consistent with observations in maize. That is when the GUS gene is regulated by the 1.0 kb ZmCAS1 promoter, incubation at 37 C resulted in increased Gus activity even in the absence of safener treatment.

Sequence CWU 1

1

221477DNAartificial sequenceZmCAS1C-1 insert 1gtacctccga caatactacc agacaaggtc cgcccaccac ggttgaggct cgcagggtgc 60atcttgatct cacttggaaa gcacactatt gtcatcacac cgccaacctt aaggagcgag 120agatacggat cgaatggatg gtcaccagaa gcagtgtcaa ctatgaagtg cagggagttt 180ttcagggcct ccatctgctg cgtgtctgac gatataacaa agttatctgc tccaaggagg 240ttaatggctt cttccctctt cgattcgctc gtgctgaaaa ccgtgacctt gagaccaaag 300gccttaccaa atttgaccgc catgtggcct angccaccga gcccaatgac tcccagcgac 360ttcccaggct gcttcatgtt gtgccgcgcc attggggtat agacggtgat tccagcacac 420anaagaagcg ctgcctgcgc cagagggtaa ccatcgggta tcttgaaaca atacctc 4772438DNAartificial sequenceZmCAS1C-2insert 2gggggnaggg gcttttnnng ganganaatg tnagtggngt atccccccnt tgtgaagggn 60ccancaacaa tnnttgtgtt gtaaatnaaa aacgnttccg ggnaatnatt ncccnaaaan 120ccgntncnat tnccccnatn gccggaacaa tttnagttag gtncnaaccc ctgnttggtc 180accaaccttt gaaaggcctt gacgtctgcg ccaaacctca atcacgaacc caagctntct 240catgcgcagg aancanagga taaactgagt cgttgtgctt gttctgtatc cacataacgt 300caccgtaaca caccccgcag tatatgatct tcagcgaaac gtcgctgctt tgcactgccc 360tgcggttaaa cttgtatggt gagagaaccc agaagggtct ctggccgccc aagcatcgca 420nttgccacct cgggcgct 43831354DNAartificial seqeunceZmCAScDNA insert 3gtcgacccac gcgtccgcgg acgctgggca gacacagact ccaccacccc gcttcgatct 60tcttgttgca gctgaaatct gtcagattct gcagttcatt ccaaatggct gctgcagcag 120agcacggcaa ctgcgatgct tgggcggcca gagacccttc tggggttctc tcaccataca 180agtttaaccg cagggcagtg caaagcagcg acgtttcgct gaagatcata tactgcgggg 240tgtgttacgg tgacgttatg tggatacaga acaagcacaa cgactcagtt tatcctctgg 300ttcctgggca tgagatagct ggggttgtga ctgaggttgg cgcagacgtc aaggccttca 360aggtgggtga ccacgcaggc gttggaacct acgtgaactc gtgccggcac tgcgagaact 420gcaacagctc tctggagaac tactgcccag aaacagtttt cacttacaac acaactgatg 480ctgatgggac catcacaaag gggggctact ccactcacat tgtcgtccat gaaaggtact 540gcttcaagat acccgatggc taccctctgg cgcaggcagc gcctcttctg tgtgctggaa 600tcaccgtcta taccccaatg gcgcggcaca acatgaagca gcctgggaag tcgctgggag 660tcattgggct cggtggccta ggccacatgg cggtcaaatt tggtaaggcc tttggtctca 720aggtcacggt tttcagcacg agcgaatcga agagggaaga agccattaac ctccttggag 780cagataactt tgttatatcg tcagacacgc agcagatgga ggccctgaaa aactccctgc 840acttcatagt tgacactgct tctggtgacc atccattcga tccgtatctc tcgctcctta 900aggttggcgg tgtgatgaca atagtgtgct ttccaagtga gatcaagatg caccctgcga 960gcctcaaccg tggtgggcgg accttgtctg gtagtattgt cggaggtaca aaagacatcc 1020aggagatggt taacttttgc gcggagaaca aaatctatcc agagatcgag atcatcaaga 1080tggattatat caacgaggct ctcgccaggc ttgttaaccg agacgtgaaa taccgctttg 1140tcatcgacat caagaactct ttcgagtagc atgctcattc acatgatccc tgtcttcttt 1200gtcaatgtat gagagataat gagtcgtttc gaataaagcg tagacatgat aaataacaag 1260tatgcttgtg attgtaaaat cgttctataa ataaatgcgc tgtgttgaac gttaaaaaaa 1320aaaaaaaaaa aaaaaaaaaa aaaagggcgg ccgc 135441338DNAZea mays 4ccacgcgtcc gcggacgctg ggcagacaca gactccacca ccccgcttcg atcttcttgt 60tgcagctgaa atctgtcaga ttctgcagtt cattccaaat ggctgctgca gcagagcacg 120gcaactgcga tgcttgggcg gccagagacc cttctggggt tctctcacca tacaagttta 180accgcagggc agtgcaaagc agcgacgttt cgctgaagat catatactgc ggggtgtgtt 240acggtgacgt tatgtggata cagaacaagc acaacgactc agtttatcct ctggttcctg 300ggcatgagat agctggggtt gtgactgagg ttggcgcaga cgtcaaggcc ttcaaggtgg 360gtgaccacgc aggcgttgga acctacgtga actcgtgccg gcactgcgag aactgcaaca 420gctctctgga gaactactgc ccagaaacag ttttcactta caacacaact gatgctgatg 480ggaccatcac aaaggggggc tactccactc acattgtcgt ccatgaaagg tactgcttca 540agatacccga tggctaccct ctggcgcagg cagcgcctct tctgtgtgct ggaatcaccg 600tctatacccc aatggcgcgg cacaacatga agcagcctgg gaagtcgctg ggagtcattg 660ggctcggtgg cctaggccac atggcggtca aatttggtaa ggcctttggt ctcaaggtca 720cggttttcag cacgagcgaa tcgaagaggg aagaagccat taacctcctt ggagcagata 780actttgttat atcgtcagac acgcagcaga tggaggccct gaaaaactcc ctgcacttca 840tagttgacac tgcttctggt gaccatccat tcgatccgta tctctcgctc cttaaggttg 900gcggtgtgat gacaatagtg tgctttccaa gtgagatcaa gatgcaccct gcgagcctca 960accgtggtgg gcggaccttg tctggtagta ttgtcggagg tacaaaagac atccaggaga 1020tggttaactt ttgcgcggag aacaaaatct atccagagat cgagatcatc aagatggatt 1080atatcaacga ggctctcgcc aggcttgtta accgagacgt gaaataccgc tttgtcatcg 1140acatcaagaa ctctttcgag tagcatgctc attcacatga tccctgtctt ctttgtcaat 1200gtatgagaga taatgagtcg tttcgaataa agcgtagaca tgataaataa caagtatgct 1260tgtgattgta aaatcgttct ataaataaat gcgctgtgtt gaacgttaaa aaaaaaaaaa 1320aaaaaaaaaa aaaaaaaa 13385354PRTZea mays 5Met Ala Ala Ala Ala Glu His Gly Asn Cys Asp Ala Trp Ala Ala Arg 1 5 10 15 Asp Pro Ser Gly Val Leu Ser Pro Tyr Lys Phe Asn Arg Arg Ala Val 20 25 30 Gln Ser Ser Asp Val Ser Leu Lys Ile Ile Tyr Cys Gly Val Cys Tyr 35 40 45 Gly Asp Val Met Trp Ile Gln Asn Lys His Asn Asp Ser Val Tyr Pro 50 55 60 Leu Val Pro Gly His Glu Ile Ala Gly Val Val Thr Glu Val Gly Ala 65 70 75 80 Asp Val Lys Ala Phe Lys Val Gly Asp His Ala Gly Val Gly Thr Tyr 85 90 95 Val Asn Ser Cys Arg His Cys Glu Asn Cys Asn Ser Ser Leu Glu Asn 100 105 110 Tyr Cys Pro Glu Thr Val Phe Thr Tyr Asn Thr Thr Asp Ala Asp Gly 115 120 125 Thr Ile Thr Lys Gly Gly Tyr Ser Thr His Ile Val Val His Glu Arg 130 135 140 Tyr Cys Phe Lys Ile Pro Asp Gly Tyr Pro Leu Ala Gln Ala Ala Pro 145 150 155 160 Leu Leu Cys Ala Gly Ile Thr Val Tyr Thr Pro Met Ala Arg His Asn 165 170 175 Met Lys Gln Pro Gly Lys Ser Leu Gly Val Ile Gly Leu Gly Gly Leu 180 185 190 Gly His Met Ala Val Lys Phe Gly Lys Ala Phe Gly Leu Lys Val Thr 195 200 205 Val Phe Ser Thr Ser Glu Ser Lys Arg Glu Glu Ala Ile Asn Leu Leu 210 215 220 Gly Ala Asp Asn Phe Val Ile Ser Ser Asp Thr Gln Gln Met Glu Ala 225 230 235 240 Leu Lys Asn Ser Leu His Phe Ile Val Asp Thr Ala Ser Gly Asp His 245 250 255 Pro Phe Asp Pro Tyr Leu Ser Leu Leu Lys Val Gly Gly Val Met Thr 260 265 270 Ile Val Cys Phe Pro Ser Glu Ile Lys Met His Pro Ala Ser Leu Asn 275 280 285 Arg Gly Gly Arg Thr Leu Ser Gly Ser Ile Val Gly Gly Thr Lys Asp 290 295 300 Ile Gln Glu Met Val Asn Phe Cys Ala Glu Asn Lys Ile Tyr Pro Glu 305 310 315 320 Ile Glu Ile Ile Lys Met Asp Tyr Ile Asn Glu Ala Leu Ala Arg Leu 325 330 335 Val Asn Arg Asp Val Lys Tyr Arg Phe Val Ile Asp Ile Lys Asn Ser 340 345 350 Phe Glu 6354PRTZea mays 6Met Ala Ala Ala Ala Glu His Gly Asn Cys Asp Ala Trp Ala Ala Arg 1 5 10 15 Asp Pro Ser Gly Val Leu Ser Pro Tyr Lys Phe Asn Arg Arg Ala Val 20 25 30 Gln Ser Ser Asp Val Ser Leu Lys Ile Ile Tyr Cys Gly Val Cys Tyr 35 40 45 Gly Asp Val Met Trp Ile Gln Asn Lys His Asn Asp Ser Val Tyr Pro 50 55 60 Leu Val Pro Gly His Glu Ile Ala Gly Val Val Thr Glu Val Gly Ala 65 70 75 80 Asp Val Lys Ala Phe Lys Val Gly Asp His Ala Gly Val Gly Thr Tyr 85 90 95 Val Asn Ser Cys Arg His Cys Glu Asn Cys Asn Ser Ser Leu Glu Asn 100 105 110 Tyr Cys Pro Glu Thr Val Phe Thr Tyr Asn Thr Thr Asp Ala Asp Gly 115 120 125 Thr Ile Thr Lys Gly Gly Tyr Ser Thr His Ile Val Val His Glu Arg 130 135 140 Tyr Cys Phe Lys Ile Pro Asp Gly Tyr Pro Leu Ala Lys Ala Ala Pro 145 150 155 160 Leu Leu Cys Ala Gly Ile Thr Val Tyr Thr Pro Met Ala Arg His Asn 165 170 175 Met Lys Gln Pro Gly Lys Ser Leu Gly Val Ile Gly Leu Gly Gly Leu 180 185 190 Gly His Met Ala Val Lys Phe Gly Lys Ala Phe Gly Leu Lys Val Thr 195 200 205 Val Phe Ser Thr Ser Glu Ser Lys Arg Glu Glu Ala Ile Asn Leu Leu 210 215 220 Gly Ala Asp Asn Phe Val Ile Ser Ser Asp Thr Gln Gln Met Glu Ala 225 230 235 240 Leu Lys Asn Ser Leu His Phe Ile Val Asp Thr Ala Ser Gly Asp His 245 250 255 Pro Phe Asp Pro Tyr Leu Ser Leu Leu Lys Val Gly Gly Val Met Thr 260 265 270 Ile Val Cys Phe Pro Ser Glu Ile Lys Met His Pro Ala Ser Leu Asn 275 280 285 Arg Gly Gly Arg Thr Leu Ser Gly Ser Ile Val Gly Gly Thr Lys Asp 290 295 300 Ile Gln Glu Met Val Asn Phe Cys Ala Glu Asn Lys Ile Tyr Pro Glu 305 310 315 320 Ile Glu Ile Ile Lys Met Asp Tyr Ile Asn Glu Ala Leu Ala Arg Leu 325 330 335 Val Asn Arg Asp Val Lys Tyr Arg Phe Val Ile Asp Ile Lys Asn Ser 340 345 350 Phe Glu 74069DNAZea mays 7cctcttaagg tcctggtaca tctcctcgat caacggcttc cacagacgaa ccctcgcgtt 60tatgaaccaa ttcgaaacct ggtacaaacg caaaaccact cgaatccatc attaccccat 120ctcgccaaaa aagaatcgaa tttgaaacga gtctggtggg atttcgatct gtgcctggtt 180cctggtgagg ctgctccttg ccgccagcac gtccttctca tggtccttgg gatacctgag 240cagagcagag cagagcgcga ttctgatcat ggccaacaat gtcgatagac gatttgcgca 300acaacgtcgc gggacagact tacgggtgca ggaagttctc gaacatccac gccttgagca 360cggcaacaga cttctctggc agcccgcact ggggccgcca gcactgctgc tcggtgcgcc 420acagctgctg cgccgaccag tgcttctgta tgaaggcgac tccagctctg ctccacgtcg 480ccgcccgcgg ccaccgtcac cgacgacgac gactcgcccc agcagctggc cctgctcgcc 540gtagccatga tctcgccggc gagccaccgc ctcagcccac ggtacatcgc cgacacggcg 600cggtgcgcga acggcgcgca gatgccaccg ccgcccgggg gcgagtgcat cagggtgttg 660aacttggctg tcgtgctctg gatcttgtcc aagcactggt tactcgtcca tctgcaaacc 720atcatccaaa tccaacatgc ttcacaaatc tgctccagaa ttcatataac taatagtgtg 780aacgaacgag tggtcaccag gaagaactcc aacatgcttc acaaaccatc gtccaactcc 840aacctggcat gtggccgacg acgtcgttga gcaaatcctg gaccacggct gcgtaccgcg 900agcgcaccac caccgcggtg aagtgcagcg ccatccgcga ccgcgaccgc gcccgcggca 960gctcggtcag ggcggaccgg ctcgctcccg aggagcacta ctccgtcgcg ttcagcgtgc 1020tgtccgagga agcgatggag ttggagcaca gcgtcggctc gctcgccacc accgccgcgg 1080acgcgttggc gccactgcag gcggcgagcg ggtagtggag gcctcctgcg tcggagagcc 1140tggccccggc gaggtggtat gtctcatggg ccggcacggg tactggtccg ccggcgctgg 1200tccagatacc gtacggcttc ttggacgacg accagaccga catggccccg gattggctgg 1260ggcaccgcaa gctcgccgcg acgcggctgt cgcagccggc gacgccgtac agctcgtcgt 1320ccatctcgtc cggcggcgtc ctcccgccgg cacgatcatc tcgcggcggc gcgggggcgt 1380tagcgaggag ctgggcagct ggctggcgaa ggaggccagc acgatgttgt tcgccgcgta 1440gtgcgcgccc agatccgccg acgaccgccg tgttgccgag gccgaacccg aagccgccct 1500ggtcaagaag cacgcagggg tggaagtgcg gcacctcagc gctcataatg ccgccacctc 1560tgtagcccag gccgaggcgg aggatggatg ggactcgaat ccgggggcgg aggtggagga 1620cccttccatg gagaccgagc ggggtttagg gatgagcgag catcacatac gccttccatg 1680gagagcatgg atgcggacgc ggatccgggg cggaagatgg cagggacgcg gattcagggc 1740ggacgcgctt gccgagggcg cgggggacca cagcgtgcgt tacggggaca gggcgggcat 1800cgcgaggacg ggtgcgggag cggagccaca tctggtggtg gacgcctact ttgctctctt 1860atagagtagt aaagattcgt ggaccaaaca acaccctagc ttgtacaaat attcttaggc 1920agttgctact gatgagagaa aaataacatc actccactgc atttgcgtga tttattgaac 1980agatcacaat tacatctatt caaatttatt tacctgtacg tgtccgattt ttaggggagg 2040atttttttac ggtatttttt ttttaaaaaa ataaatttag gcaacaattt tatagaatcg 2100agtgctttat ctattatctt ttacaaggca cacgcgtaca ataaggtttg gtcgttcgtg 2160acttggatag tggttttggt tgcaattccg taattcttgg cataggatac agcccaaccc 2220agaaaaaaat aatgttgcgg tcagttctgg ctttgagatt cggagtacca cgtggcgtaa 2280aggcaggccg tgtcttacag atgaataaag gacctgggtc tcacgtgatt ggtttccagt 2340ttcgtgcatc aagatgtgga attttcaaac tgccgtcgtg tttgtttcgt cacataaaag 2400ctttttggaa ggctaaggag aggaagccgg cgagaaggag ggggcgtttt acgtgtcact 2460gtcctgtcgt gttggctgtt gacacgaatc atttcttccg cgcgtgggaa gaagaagatg 2520cacattagcg gcctgaagta gagatgtcaa tggggaattc cccagcgggg attaactccc 2580cagacccgta cccatgaaca tagaccggcc cccatccccg aacccgaacc cgacctcggg 2640tacgaaaatc ctcccatacc cattcccgac cgggtactaa atacccatgg gtatccatac 2700ccgacccgat tattcaaaaa ttaatgggct ttttatttgt taaccggcgg acgcaatgct 2760tgggactcta ggttttttta ctttgttgac cggctggcgg ctgggctttt tcctacaggc 2820ccaaagttgg tcggcagcca ctaggccaca cgtcacaggc agcccacaag taaatgtcgt 2880tggattgctg gatggtggaa taaaaatcct agatgctaga ttgttctggt tccgggtatt 2940tttctccatg gctaatcggg tttgggttta gccctcccaa acccgaaccc gccatacccg 3000atgggtaagg gatttattcc aaatctatac ccatggggat ttgttttaac ccatacctta 3060accctaatag aggaattccc cacgggtaat cgggtttcgg ggcccattga catctctaga 3120ctgaaggcgt ccaactcaaa tcattaaaaa gtgttgacgc acgcgctgat gcgccggccg 3180cacagcacag gctgcacagc ccgtttaatc agcgatggag ccccggccgt cagccagcca 3240ggtccggcgt ccgggtctgc gccctgcggc gtcactgctg tcgccaccgt ctccgatggt 3300cccacatcca tccagcgggc cgcgcgtggt acaaaaggct cttcctcgcc gtcaggtgca 3360gctgcccaaa caccagacac agactccacc accccgcttc gatcttctgt tgcagctgaa 3420atctgtcaga ttctgcagtt cattccaaat ggctgctgca gcagagcacg gcaactgcga 3480tgcttgggcg gccagagacc cttctggggt tctctcacca tacaagttta accgcaggtg 3540aggtggctcc ccctcctcat ttggtgcaca actcatcatc atcatgtttt tttatcgcct 3600tttcagaaca ctgctgtctg tcttctcgct atgctagaat tctttggaat agctgccagt 3660gttgttttcc ttgcctttta catgtacatc atgttttttt tcctgcctgt ggagtgggtt 3720gatttaaatc actagcaagt caaaaattat ttctaatttc tttcgatctc attaaattca 3780cacagaacgg gaataaccga acacagccta aaatcgtgaa actgatgcat agcagacggt 3840agaccatccg taaccgtaca tattcataac gtctactatg ccttctggta tttttttttt 3900ccagggcagt gcaaagcagc gacgtttcgc tgaagatcat atactgcggg gtgtgttacg 3960gtgacgttat gtggatacag aacaagcaca acgactcagt ttatcctctg gttcctgggt 4020aagaacatgt acactgaacc cctagcttag tagagctagg tagggagct 4069833DNAartificial sequenceCAS1_RCA_MUTAGENESIS_PRIMER 8gcagttcatt cctcatgact gctgcagcag agc 3391049DNAZea mays 9aagctttttg gaaggctaag gagaggaagc cggcgagaag gagggggcgt tttacgtgtc 60actgtcctgt cgtgttggct gttgacacga atcatttctt ccgcgcgtgg gaagaagaag 120atgcacatta gcggcctgaa gtagagatgt caatggggaa ttccccagcg gggattaact 180ccccagaccc gtacccatga acatagaccg gcccccatcc ccgaacccga acccgacctc 240gggtacgaaa atcctcccat acccattccc gaccgggtac taaataccca tgggtatcca 300tacccgaccc gattattcaa aaattaatgg gctttttatt tgttaaccgg cggacgcaat 360gcttgggact ctaggttttt ttactttgtt gaccggctgg cggctgggct ttttcctaca 420ggcccaaagt tggtcggcag ccactaggcc acacgtcaca ggcagcccac aagtaaatgt 480cgttggattg ctggatggtg gaataaaaat cctagatgct agattgttct ggttccgggt 540atttttctcc atggctaatc gggtttgggt ttagccctcc caaacccgaa cccgccatac 600ccgatgggta agggatttat tccaaatcta tacccatggg gatttgtttt aacccatacc 660ttaaccctaa tagaggaatt ccccacgggt aatcgggttt cggggcccat tgacatctct 720agactgaagg cgtccaactc aaatcattaa aaagtgttga cgcacgcgct gatgcgccgg 780ccgcacagca caggctgcac agcccgttta atcagcgatg gagccccggc cgtcagccag 840ccaggtccgg cgtccgggtc tgcgccctgc ggcgtcactg ctgtcgccac cgtctccgat 900ggtcccacat ccatccagcg ggccgcgcgt ggtacaaaag gctcttcctc gccgtcaggt 960gcagctgccc aaacaccaga cacagactcc accaccccgc ttcgatcttc tgttgcagct 1020gaaatctgtc agattctgca gttcattcc 1049101746DNAZea mays 10ggatccgggg cggaagatgg cagggacgcg gattcagggc ggacgcgctt gccgagggcg 60cgggggacca cagcgtgcgt tacggggaca gggcgggcat cgcgaggacg ggtgcgggag 120cggagccaca tctggtggtg gacgcctact ttgctctctt atagagtagt aaagattcgt 180ggaccaaaca acaccctagc ttgtacaaat attcttaggc agttgctact gatgagagaa 240aaataacatc actccactgc atttgcgtga tttattgaac agatcacaat tacatctatt 300caaatttatt tacctgtacg tgtccgattt ttaggggagg atttttttac ggtatttttt 360ttttaaaaaa ataaatttag gcaacaattt tatagaatcg agtgctttat ctattatctt 420ttacaaggca cacgcgtaca ataaggtttg gtcgttcgtg acttggatag tggttttggt 480tgcaattccg taattcttgg cataggatac agcccaaccc agaaaaaaat aatgttgcgg 540tcagttctgg ctttgagatt cggagtacca cgtggcgtaa aggcaggccg tgtcttacag 600atgaataaag gacctgggtc tcacgtgatt ggtttccagt ttcgtgcatc aagatgtgga 660attttcaaac tgccgtcgtg tttgtttcgt cacataaaag ctttttggaa ggctaaggag 720aggaagccgg cgagaaggag ggggcgtttt acgtgtcact gtcctgtcgt gttggctgtt 780gacacgaatc atttcttccg cgcgtgggaa gaagaagatg cacattagcg gcctgaagta 840gagatgtcaa tggggaattc cccagcgggg attaactccc cagacccgta cccatgaaca 900tagaccggcc cccatccccg aacccgaacc cgacctcggg tacgaaaatc ctcccatacc 960cattcccgac cgggtactaa atacccatgg gtatccatac ccgacccgat tattcaaaaa 1020ttaatgggct ttttatttgt taaccggcgg acgcaatgct tgggactcta ggttttttta 1080ctttgttgac cggctggcgg ctgggctttt tcctacaggc ccaaagttgg tcggcagcca 1140ctaggccaca cgtcacaggc agcccacaag taaatgtcgt tggattgctg gatggtggaa 1200taaaaatcct agatgctaga ttgttctggt tccgggtatt

tttctccatg gctaatcggg 1260tttgggttta gccctcccaa acccgaaccc gccatacccg atgggtaagg gatttattcc 1320aaatctatac ccatggggat ttgttttaac ccatacctta accctaatag aggaattccc 1380cacgggtaat cgggtttcgg ggcccattga catctctaga ctgaaggcgt ccaactcaaa 1440tcattaaaaa gtgttgacgc acgcgctgat gcgccggccg cacagcacag gctgcacagc 1500ccgtttaatc agcgatggag ccccggccgt cagccagcca ggtccggcgt ccgggtctgc 1560gccctgcggc gtcactgctg tcgccaccgt ctccgatggt cccacatcca tccagcgggc 1620cgcgcgtggt acaaaaggct cttcctcgcc gtcaggtgca gctgcccaaa caccagacac 1680agactccacc accccgcttc gatcttctgt tgcagctgaa atctgtcaga ttctgcagtt 1740cattcc 17461147747DNAartificial sequencePHP16974 ZmCAS1HindIII ProGUS/35SPAT 11tctagagctc gttcctcgag gaacggtacc tgcggggaag cttacaataa tgtgtgttgt 60taagtcttgt tgcctgtcat cgtctgactg actttcgtca taaatcccgg cctccgtaac 120ccagctttgg gcaagctcac ggatttgatc cggcggaacg ggaatatcga gatgccgggc 180tgaacgctgc agttccagct ttccctttcg ggacaggtac tccagctgat tgattatctg 240ctgaagggtc ttggttccac ctcctggcac aatgcgaatg attacttgag cgcgatcggg 300catccaattt tctcccgtca ggtgcgtggt caagtgctac aaggcacctt tcagtaacga 360gcgaccgtcg atccgtcgcc gggatacgga caaaatggag cgcagtagtc catcgagggc 420ggcgaaagcc tcgccaaaag caatacgttc atctcgcaca gcctccagat ccgatcgagg 480gtcttcggcg taggcagata gaagcatgga tacattgctt gagagtattc cgatggactg 540aagtatggct tccatctttt ctcgtgtgtc tgcatctatt tcgagaaagc ccccgatgcg 600gcgcaccgca acgcgaattg ccatactatc cgaaagtccc agcaggcgcg cttgatagga 660aaaggtttca tactcggccg atcgcagacg ggcactcacg accttgaacc cttcaacttt 720cagggatcga tgctggttga tggtagtctc actcgacgtg gctctggtgt gttttgacat 780agcttcctcc aaagaaagcg gaaggtctgg atactccagc acgaaatgtg cccgggtaga 840cggatggaag tctagccctg ctcaatatga aatcaacagt acatttacag tcaatactga 900atatacttgc tacatttgca attgtcttat aacgaatgtg aaataaaaat agtgtaacaa 960cgcttttact catcgataat cacaaaaaca tttatacgaa caaaaataca aatgcactcc 1020ggtttcacag gataggcggg atcagaatat gcaacttttg acgttttgtt ctttcaaagg 1080gggtgctggc aaaaccaccg cactcatggg cctttgcgct gctttggcaa atgacggtaa 1140acgagtggcc ctctttgatg ccgacgaaaa ccggcctctg acgcgatgga gagaaaacgc 1200cttacaaagc agtactggga tcctcgctgt gaagtctatt ccgccgacga aatgcccctt 1260cttgaagcag cctatgaaaa tgccgagctc gaaggatttg attatgcgtt ggccgatacg 1320cgtggcggct cgagcgagct caacaacaca atcatcgcta gctcaaacct gcttctgatc 1380cccaccatgc taacgccgct cgacatcgat gaggcactat ctacctaccg ctacgtcatc 1440gagctgctgt tgagtgaaaa tttggcaatt cctacagctg ttttgcgcca acgcgtcccg 1500gtcggccgat tgacaacatc gcaacgcagg atgtcagaga cgctagagag ccttccagtt 1560gtaccgtctc ccatgcatga aagagatgca tttgccgcga tgaaagaacg cggcatgttg 1620catcttacat tactaaacac gggaactgat ccgacgatgc gcctcataga gaggaatctt 1680cggattgcga tggaggaagt cgtggtcatt tcgaaactga tcagcaaaat cttggaggct 1740tgaagatggc aattcgcaag cccgcattgt cggtcggcga agcacggcgg cttgctggtg 1800ctcgacccga gatccaccat cccaacccga cacttgttcc ccagaagctg gacctccagc 1860acttgcctga aaaagccgac gagaaagacc agcaacgtga gcctctcgtc gccgatcaca 1920tttacagtcc cgatcgacaa cttaagctaa ctgtggatgc ccttagtcca cctccgtccc 1980cgaaaaagct ccaggttttt ctttcagcgc gaccgcccgc gcctcaagtg tcgaaaacat 2040atgacaacct cgttcggcaa tacagtccct cgaagtcgct acaaatgatt ttaaggcgcg 2100cgttggacga tttcgaaagc atgctggcag atggatcatt tcgcgtggcc ccgaaaagtt 2160atccgatccc ttcaactaca gaaaaatccg ttctcgttca gacctcacgc atgttcccgg 2220ttgcgttgct cgaggtcgct cgaagtcatt ttgatccgtt ggggttggag accgctcgag 2280ctttcggcca caagctggct accgccgcgc tcgcgtcatt ctttgctgga gagaagccat 2340cgagcaattg gtgaagaggg acctatcgga acccctcacc aaatattgag tgtaggtttg 2400aggccgctgg ccgcgtcctc agtcaccttt tgagccagat aattaagagc caaatgcaat 2460tggctcaggc tgccatcgtc cccccgtgcg aaacctgcac gtccgcgtca aagaaataac 2520cggcacctct tgctgttttt atcagttgag ggcttgacgg atccgcctca agtttgcggc 2580gcagccgcaa aatgagaaca tctatactcc tgtcgtaaac ctcctcgtcg cgtactcgac 2640tggcaatgag aagttgctcg cgcgatagaa cgtcgcgggg tttctctaaa aacgcgagga 2700gaagattgaa ctcacctgcc gtaagtttca cctcaccgcc agcttcggac atcaagcgac 2760gttgcctgag attaagtgtc cagtcagtaa aacaaaaaga ccgtcggtct ttggagcgga 2820caacgttggg gcgcacgcgc aaggcaaccc gaatgcgtgc aagaaactct ctcgtactaa 2880acggcttagc gataaaatca cttgctccta gctcgagtgc aacaacttta tccgtctcct 2940caaggcggtc gccactgata attatgattg gaatatcaga ctttgccgcc agatttcgaa 3000cgatctcaag cccatcttca cgacctaaat ttagatcaac aaccacgaca tcgaccgtcg 3060cggaagagag tactctagtg aactgggtgc tgtcggctac cgcggtcact ttgaaggcgt 3120ggatcgtaag gtattcgata ataagatgcc gcatagcgac atcgtcatcg ataagaagaa 3180cgtgtttcaa cggctcacct ttcaatctaa aatctgaacc cttgttcaca gcgcttgaga 3240aattttcacg tgaaggatgt acaatcatct ccagctaaat gggcagttcg tcagaattgc 3300ggctgaccgc ggatgacgaa aatgcgaacc aagtatttca attttatgac aaaagttctc 3360aatcgttgtt acaagtgaaa cgcttcgagg ttacagctac tattgattaa ggagatcgcc 3420tatggtctcg ccccggcgtc gtgcgtccgc cgcgagccag atctcgccta cttcataaac 3480gtcctcatag gcacggaatg gaatgatgac atcgatcgcc gtagagagca tgtcaatcag 3540tgtgcgatct tccaagctag caccttgggc gctacttttg acaagggaaa acagtttctt 3600gaatccttgg attggattcg cgccgtgtat tgttgaaatc gatcccggat gtcccgagac 3660gacttcactc agataagccc atgctgcatc gtcgcgcatc tcgccaagca atatccggtc 3720cggccgcata cgcagacttg cttggagcaa gtgctcggcg ctcacagcac ccagcccagc 3780accgttcttg gagtagagta gtctaacatg attatcgtgt ggaatgacga gttcgagcgt 3840atcttctatg gtgattagcc tttcctgggg ggggatggcg ctgatcaagg tcttgctcat 3900tgttgtcttg ccgcttccgg tagggccaca tagcaacatc gtcagtcggc tgacgacgca 3960tgcgtgcaga aacgcttcca aatccccgtt gtcaaaatgc tgaaggatag cttcatcatc 4020ctgattttgg cgtttccttc gtgtctgcca ctggttccac ctcgaagcat cataacggga 4080ggagacttct ttaagaccag aaacacgcga gcttggccgt cgaatggtca agctgacggt 4140gcccgaggga acggtcggcg gcagacagat ttgtagtcgt tcaccaccag gaagttcagt 4200ggcgcagagg gggttacgtg gtccgacatc ctgctttctc agcgcgcccg ctaaaatagc 4260gatatcttca agatcatcat aagagacggg caaaggcatc ttggtaaaaa tgccggcttg 4320gcgcacaaat gcctctccag gtcgattgat cgcaatttct tcagtcttcg ggtcatcgag 4380ccattccaaa atcggcttca gaagaaagcg tagttgcgga tccacttcca tttacaatgt 4440atcctatctc taagcggaaa tttgaattca ttaagagcgg cggttcctcc cccgcgtggc 4500gccgccagtc aggcggagct ggtaaacacc aaagaaatcg aggtcccgtg ctacgaaaat 4560ggaaacggtg tcaccctgat tcttcttcag ggttggcggt atgttgatgg ttgccttaag 4620ggctgtctca gttgtctgct caccgttatt ttgaaagctg ttgaagctca tcccgccacc 4680cgagctgccg gcgtaggtgc tagctgcctg gaaggcgcct tgaacaacac tcaagagcat 4740agctccgcta aaacgctgcc agaagtggct gtcgaccgag cccggcaatc ctgagcgacc 4800gagttcgtcc gcgcttggcg atgttaacga gatcatcgca tggtcaggtg tctcggcgcg 4860atcccacaac acaaaaacgc gcccatctcc ctgttgcaag ccacgctgta tttcgccaac 4920aacggtggtg ccacgatcaa gaagcacgat attgttcgtt gttccacgaa tatcctgagg 4980caagacacac tttacatagc ctgccaaatt tgtgtcgatt gcggtttgca agatgcacgg 5040aattattgtc ccttgcgtta ccataaaatc ggggtgcggc aagagcgtgg cgctgctggg 5100ctgcagctcg gtgggtttca tacgtatcga caaatcgttc tcgccggaca cttcgccatt 5160cggcaaggag ttgtcgtcac gcttgccttc ttgtcttcgg cccgtgtcgc cctgaatggc 5220gcgtttgctg accccttgat cgccgctgct atatgcaaaa atcggtgttt cttccggccg 5280tggctcatgc cgctccggtt cgcccctcgg cggtagagga gcagcaggct gaacagcctc 5340ttgaaccgct ggaggatccg gcggcacctc aatcggagct ggatgaaatg gcttggtgtt 5400tgttgcgatc aaagttgacg gcgatgcgtt ctcattcacc ttcttttggc gcccacctag 5460ccaaatgagg cttaatgata acgcgagaac gacacctccg acgatcaatt tctgagaccc 5520cgaaagacgc cggcgatgtt tgtcggagac cagggatcca gatgcatcaa cctcatgtgc 5580cgcttgctga ctatcgttat tcatcccttc gcccccttca ggacgcgttt cacatcgggc 5640ctcaccgtgc ccgtttgcgg cctttggcca acgggatcgt aagcggtgtt ccagatacat 5700agtactgtgt ggccatccct cagacgccaa cctcgggaaa ccgaagaaat ctcgacatcg 5760ctccctttaa ctgaatagtt ggcaacagct tccttgccat caggattgat ggtgtagatg 5820gagggtatgc gtacattgcc cggaaagtgg aataccgtcg taaatccatt gtcgaagact 5880tcgagtggca acagcgaacg atcgccttgg gcgacgtagt gccaattact gtccgccgca 5940ccaagggctg tgacaggctg atccaataaa ttctcagctt tccgttgata ttgtgcttcc 6000gcgtgtagtc tgtccacaac agccttctgt tgtgcctccc ttcgccgagc cgccgcatcg 6060tcggcggggt aggcgaattg gacgctgtaa tagagatcgg gctgctcttt atcgaggtgg 6120gacagagtct tggaacttat actgaaaaca taacggcgca tcccggagtc gcttgcggtt 6180agcacgatta ctggctgagg cgtgaggacc tggcttgcct tgaaaaatag ataatttccc 6240cgcggtaggg ctgctagatc tttgctattt gaaacggcaa ccgctgtcac cgtttcgttc 6300gtggcgaatg ttacgaccaa agtagctcca accgccgtcg agaggcgcac cacttgatcg 6360ggattgtaag ccaaataacg catgcgcgga tctagcttgc ccgccattgg agtgtcttca 6420gcctccgcac cagtcgcagc ggcaaataaa catgctaaaa tgaaaagtgc ttttctgatc 6480atggttcgct gtggcctacg tttgaaacgg tatcttccga tgtctgatag gaggtgacaa 6540ccagacctgc cgggttggtt agtctcaatc tgccgggcaa gctggtcacc ttttcgtagc 6600gaactgtcgc ggtccacgta ctcaccacag gcattttgcc gtcaacgacg agggtccttt 6660tatagcgaat ttgctgcgtg cttggagtta catcatttga agcgatgtgc tcgacctcca 6720ccctgccgcg tttgccaaga atgacttgag gcgaactggg attgggatag ttgaagaatt 6780gctggtaatc ctggcgcact gttggggcac tgaagttcga taccaggtcg taggcgtact 6840gagcggtgtc ggcatcataa ctctcgcgca ggcgaacgta ctcccacaat gaggcgttaa 6900cgacggcctc ctcttgagtt gcaggcaatc gcgagacaga cacctcgctg tcaacggtgc 6960cgtccggccg tatccataga tatacgggca caagcctgct caacggcacc attgtggcta 7020tagcgaacgc ttgagcaaca tttcccaaaa tcgcgatagc tgcgacagct gcaatgagtt 7080tggagagacg tcgcgccgat ttcgctcgcg cggtttgaaa ggcttctact tccttatagt 7140gctcggcaag gctttcgcgc gccactagca tggcatattc aggccccgtc atagcgtcca 7200cccgaattgc cgagctgaag atctgacgga gtaggctgcc atcgccccac attcagcggg 7260aagatcgggc ctttgcagct cgctaatgtg tcgtttgtct ggcagccgct caaagcgaca 7320actaggcaca gcaggcaata cttcatagaa ttctccattg aggcgaattt ttgcgcgacc 7380tagcctcgct caacctgagc gaagcgacgg tacaagctgc tggcagattg ggttgcgccg 7440ctccagtaac tgcctccaat gttgccggcg atcgccggca aagcgacaat gagcgcatcc 7500cctgtcagaa aaaacatatc gagttcgtaa agaccaatga tcttggccgc ggtcgtaccg 7560gcgaaggtga ttacaccaag cataagggtg agcgcagtcg cttcggttag gatgacgatc 7620gttgccacga ggtttaagag gagaagcaag agaccgtagg tgataagttg cccgatccac 7680ttagctgcga tgtcccgcgt gcgatcaaaa atatatccga cgaggatcag aggcccgatc 7740gcgagaagca ctttcgtgag aattccaacg gcgtcgtaaa ctccgaaggc agaccagagc 7800gtgccgtaaa ggacccactg tgccccttgg aaagcaagga tgtcctggtc gttcatcgga 7860ccgatttcgg atgcgatttt ctgaaaaacg gcctgggtca cggcgaacat tgtatccaac 7920tgtgccggaa cagtctgcag aggcaagccg gttacactaa actgctgaac aaagtttggg 7980accgtctttt cgaagatgga aaccacatag tcttggtagt tagcctgccc aacaattaga 8040gcaacaacga tggtgaccgt gatcacccga gtgataccgc tacgggtatc gacttcgccg 8100cgtatgacta aaataccctg aacaataatc caaagagtga cacaggcgat caatggcgca 8160ctcaccgcct cctggatagt ctcaagcatc gagtccaagc ctgtcgtgaa ggctacatcg 8220aagatcgtat gaatggccgt aaacggcgcc ggaatcgtga aattcatcga ttggacctga 8280acttgactgg tttgtcgcat aatgttggat aaaatgagct cgcattcggc gaggatgcgg 8340gcggatgaac aaatcgccca gccttagggg agggcaccaa agatgacagc ggtcttttga 8400tgctccttgc gttgagcggc cgcctcttcc gcctcgtgaa ggccggcctg cgcggtagtc 8460atcgttaata ggcttgtcgc ctgtacattt tgaatcattg cgtcatggat ctgcttgaga 8520agcaaaccat tggtcacggt tgcctgcatg atattgcgag atcgggaaag ctgagcagac 8580gtatcagcat tcgccgtcaa gcgtttgtcc atcgtttcca gattgtcagc cgcaatgcca 8640gcgctgtttg cggaaccggt gatctgcgat cgcaacaggt ccgcttcagc atcactaccc 8700acgactgcac gatctgtatc gctggtgatc gcacgtgccg tggtcgacat tggcattcgc 8760ggcgaaaaca tttcattgtc taggtccttc gtcgaaggat actgattttt ctggttgagc 8820gaagtcagta gtccagtaac gccgtaggcc gacgtcaaca tcgtaaccat cgctatagtc 8880tgagtgagat tctccgcagt cgcgagcgca gtcgcgagcg tctcagcctc cgttgccggg 8940tcgctaacaa caaactgcgc ccgcgcgggc tgaatatata gaaagctgca ggtcaaaact 9000gttgcaataa gttgcgtcgt cttcatcgtt tcctacctta tcaatcttct gcctcgtggt 9060gacgggccat gaattcgctg agccagccag atgagttgcc ttcttgtgcc tcgcgtagtc 9120gagttgcaaa gcgcaccgtg ttggcacgcc ccgaaagcac ggcgacatat tcacgcatat 9180cccgcagatc aaattcgcag atgacgcttc cactttctcg tttaagaaga aacttacggc 9240tgccgaccgt catgtcttca cggatcgcct gaaattcctt ttcggtacat ttcagtccat 9300cgacataagc cgatcgatct gcggttggtg atggatagaa aatcttcgtc atacattgcg 9360caaccaagct ggctcctagc ggcgattcca gaacatgctc tggttgctgc gttgccagta 9420ttagcatccc gttgtttttt cgaacggtca ggaggaattt gtcgacgaca gtcgaaaatt 9480tagggtttaa caaataggcg cgaaactcat cgcagctcat cacaaaacgg cggccgtcga 9540tcatggctcc aatccgatgc aggagatatg ctgcagcggg agcgcatact tcctcgtatt 9600cgagaagatg cgtcatgtcg aagccggtaa tcgacggatc taactttact tcgtcaactt 9660cgccgtcaaa tgcccagcca agcgcatggc cccggcacca gcgttggagc cgcgctcctg 9720cgccttcggc gggcccatgc aacaaaaatt cacgtaaccc cgcgattgaa cgcatttgtg 9780gatcaaacga gagctgacga tggataccac ggaccagacg gcggttctct tccggagaaa 9840tcccaccccg accatcactc tcgatgagag ccacgatcca ttcgcgcaga aaatcgtgtg 9900aggctgctgt gttttctagg ccacgcaacg gcgccaaccc gctgggtgtg cctctgtgaa 9960gtgccaaata tgttcctcct gtggcgcgaa ccagcaattc gccaccccgg tccttgtcaa 10020agaacacgac cgtacctgca cggtcgacca tgctctgttc gagcatggct agaacaaaca 10080tcatgagcgt cgtcttaccc ctcccgatag gcccgaatat tgccgtcatg ccaacatcgt 10140gctcatgcgg gatatagtcg aaaggcgttc cgccattggt acgaaatcgg gcaatcgcgt 10200tgccccagtg gcctgagctg gcgccctctg gaaagttttc gaaagagaca aaccctgcga 10260aattgcgtga agtgattgcg ccagggcgtg tgcgccactt aaaattcccc ggcaattggg 10320accaataggc cgcttccata ccaatacctt cttggacaac cacggcacct gcatccgcca 10380ttcgtgtccg agcccgcgcg cccctgtccc caagactatt gagatcgtct gcatagacgc 10440aaaggctcaa atgatgtgag cccataacga attcgttgct cgcaagtgcg tcctcagcct 10500cggataattt gccgatttga gtcacggctt tatcgccgga actcagcatc tggctcgatt 10560tgaggctaag tttcgcgtgc gcttgcgggc gagtcaggaa cgaaaaactc tgcgtgagaa 10620caagtggaaa atcgagggat agcagcgcgt tgagcatgcc cggccgtgtt tttgcagggt 10680attcgcgaaa cgaatagatg gatccaacgt aactgtcttt tggcgttctg atctcgagtc 10740ctcgcttgcc gcaaatgact ctgtcggtat aaatcgaagc gccgagtgag ccgctgacga 10800ccggaaccgg tgtgaaccga ccagtcatga tcaaccgtag cgcttcgcca atttcggtga 10860agagcacacc ctgcttctcg cggatgccaa gacgatgcag gccatacgct ttaagagagc 10920cagcgacaac atgccaaaga tcttccatgt tcctgatctg gcccgtgaga tcgttttccc 10980tttttccgct tagcttggtg aacctcctct ttaccttccc taaagccgcc tgtgggtaga 11040caatcaacgt aaggaagtgt tcattgcgga ggagttggcc ggagagcacg cgctgttcaa 11100aagcttcgtt caggctagcg gcgaaaacac tacggaagtg tcgcggcgcc gatgatggca 11160cgtcggcatg acgtacgagg tgagcatata ttgacacatg atcatcagcg atattgcgca 11220acagcgtgtt gaacgcacga caacgcgcat tgcgcatttc agtttcctca agctcgaatg 11280caacgccatc aattctcgca atggtcatga tcgatccgtc ttcaagaagg acgatatggt 11340cgctgaggtg gccaatataa gggagataga tctcaccgga tctttcggtc gttccactcg 11400cgccgagcat cacaccattc ctctccctcg tgggggaacc ctaattggat ttgggctaac 11460agtagcgccc ccccaaactg cactatcaat gcttcttccc gcggtccgca aaaatagcag 11520gacgacgctc gccgcattgt agtctcgctc cacgatgagc cgggctgcaa accataacgg 11580cacgagaacg acttcgtaga gcgggttctg aacgataacg atgacaaagc cggcgaacat 11640catgaataac cctgccaatg tcagtggcac cccaagaaac aatgcgggcc gtgtggctgc 11700gaggtaaagg gtcgattctt ccaaacgatc agccatcaac taccgccagt gagcgtttgg 11760ccgaggaagc tcgccccaaa catgataaca atgccgccga cgacgccggc aaccagccca 11820agcgaagccc gcccgaacat ccaggagatc ccgatagcga caatgccgag aacagcgagt 11880gactggccga acggaccaag gataaacgtg catatattgt taaccattgt ggcggggtca 11940gtgccgccac ccgcagattg cgctgcggcg ggtccggatg aggaaatgct ccatgcaatt 12000gcaccgcaca agcttggggc gcagctcgat atcacgcgca tcatcgcatt cgagagcgag 12060aggcgattta gatgtaaacg gtatctctca aagcatcgca tcaatgcgca cctccttagt 12120ataagtcgaa taagacttga ttgtcgtctg cggatttgcc gttgtcctgg tgtggcggtg 12180gcggagcgat taaaccgcca gcgccatcct cctgcgagcg gcgctgatat gacccccaaa 12240catcccacgt ctcttcggat tttagcgcct cgtgatcgtc ttttggaggc tcgattaacg 12300cgggcaccag cgattgagca gctgtttcaa cttttcgcac gtagccgttt gcaaaaccgc 12360cgatgaaatt accggtgttg taagcggaga tcgcccgacg aagcgcaaat tgcttctcgt 12420caatcgtttc gccgcctgca taacgacttt tcagcatgtt tgcagcggca gataatgatg 12480tgcacgcctg gagcgcaccg tcaggtgtca gaccgagcat agaaaaattt cgagagttta 12540tttgcatgag gccaacatcc agcgaatgcc gtgcatcgag acggtgcctg acgacttggg 12600ttgcttggct gtgatcttgc cagtgaagcg tttcgccggt cgtgttgtca tgaatcgcta 12660aaggatcaaa gcgactctcc accttagcta tcgccgcaag cgtagatgtc gcaactgatg 12720gggcacactt gcgagcaaca tggtcaaact cagcagatga gagtggcgtg gcaaggctcg 12780acgaacagaa ggagaccatc aaggcaagag aaagcgaccc cgatctctta agcatacctt 12840atctccttag ctcgcaacta acaccgcctc tcccgttgga agaagtgcgt tgttttatgt 12900tgaagattat cgggagggtc ggttactcga aaattttcaa ttgcttcttt atgatttcaa 12960ttgaagcgag aaacctcgcc cggcgtcttg gaacgcaaca tggaccgaga accgcgcatc 13020catgactaag caaccggatc gacctattca ggccgcagtt ggtcaggtca ggctcagaac 13080gaaaatgctc ggcgaggtta cgctgtctgt aaacccattc gatgaacggg aagcttcctt 13140ccgattgctc ttggcaggaa tattggccca tgcctgcttg cgctttgcaa atgctcttat 13200cgcgttggta tcatatgcct tgtccgccag cagaaacgca ctctaagcga ttatttgtaa 13260aaatgtttcg gtcatgcggc ggtcatgggc ttgacccgct gtcagcgcaa gacggatcgg 13320tcaaccgtcg gcatcgacaa cagcgtgaat cttggtggtc aaaccgccac gggaacgtcc 13380catacagcca tcgtcttgat cccgctgttt cccgtcgccg catgttggtg gacgcggaca 13440caggaactgt caatcatgac gacattctat cgaaagcctt ggaaatcaca ctcagaatat 13500gatcccagac gtctgcctca cgccatcgta caaagcgatt gtagcaggtt gtacaggaac 13560cgtatcgatc aggaacgtct gcccagggcg ggcccgtccg gaagcgccac aagatgacat 13620tgatcacccg cgtcaacgcg cggcacgcga cgcggcttat ttgggaacaa aggactgaac 13680aacagtccat tcgaaatcgg tgacatcaaa gcggggacgg gttatcagtg gcctccaagt 13740caagcctcaa tgaatcaaaa tcagaccgat ttgcaaacct gatttatgag tgtgcggcct 13800aaatgatgaa atcgtccttc tagatcgcct ccgtggtgta gcaacacctc gcagtatcgc 13860cgtgctgacc ttggccaggg aattgactgg caagggtgct ttcacatgac cgctcttttg 13920gccgcgatag atgatttcgt tgctgctttg ggcacgtaga aggagagaag tcatatcgga 13980gaaattcctc ctggcgcgag agcctgctct atcgcgacgg catcccactg tcgggaacag 14040accggatcat tcacgaggcg aaagtcgtca acacatgcgt tataggcatc ttcccttgaa 14100ggatgatctt gttgctgcca atctggaggt gcggcagccg caggcagatg cgatctcagc 14160gcaacttgcg gcaaaacatc tcactcacct gaaaaccact agcgagtctc gcgatcagac 14220gaaggccttt tacttaacga cacaatatcc gatgtctgca tcacaggcgt cgctatccca 14280gtcaatacta aagcggtgca ggaactaaag attactgatg acttaggcgt gccacgaggc 14340ctgagacgac gcgcgtagac agttttttga aatcattatc aaagtgatgg cctccgctga 14400agcctatcac ctctgcgccg gtctgtcgga gagatgggca

agcattatta cggtcttcgc 14460gcccgtacat gcattggacg attgcagggt caatggatct gagatcatcc agaggattgc 14520cgcccttacc ttccgtttcg agttggagcc agcccctaaa tgagacgaca tagtcgactt 14580gatgtgacaa tgccaagaga gagatttgct taacccgatt tttttgctca agcgtaagcc 14640tattgaagct tgccggcatg acgtccgcgc cgaaagaata tcctacaagt aaaacattct 14700gcacaccgaa atgcttggtg tagacatcga ttatgtgacc aagatcctta gcagtttcgc 14760ttggggaccg ctccgaccag aaataccgaa gtgaactgac gccaatgaca ggaatccctt 14820ccgtctgcag ataggtacca tcgatagatc tgctgcctcg cgcgtttcgg tgatgacggt 14880gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 14940gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 15000atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 15060agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 15120aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 15180ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 15240gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 15300aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 15360gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 15420ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 15480cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 15540cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 15600gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 15660cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 15720agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 15780ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 15840ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 15900gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 15960cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 16020attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 16080accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 16140ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 16200gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 16260agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 16320ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 16380ttgttgccat tgctgcaggg gggggggggg ggggggactt ccattgttca ttccacggac 16440aaaaacagag aaaggaaacg acagaggcca aaaagcctcg ctttcagcac ctgtcgtttc 16500ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa 16560cgccttaaac cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac 16620ctgtcggatc accggaaagg acccgtaaag tgataatgat tatcatctac atatcacaac 16680gtgcgtggag gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat 16740tgatctgcat caacttaacg taaaaacaac ttcagacaat acaaatcagc gacactgaat 16800acggggcaac ctcatgtccc cccccccccc ccccctgcag ggcatcgtgg tgtcacgctc 16860gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 16920ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 16980gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 17040gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 17100gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 17160tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 17220gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 17280agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 17340aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 17400ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 17460gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 17520agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 17580tcttcaagaa ttggtcgacg atcttgctgc gttcggatat tttcgtggag ttcccgccac 17640agacccggat tgaaggcgag atccagcaac tcgcgccaga tcatcctgtg acggaacttt 17700ggcgcgtgat gactggccag gacgtcggcc gaaagagcga caagcagatc acgcttttcg 17760acagcgtcgg atttgcgatc gaggattttt cggcgctgcg ctacgtccgc gaccgcgttg 17820agggatcaag ccacagcagc ccactcgacc ttctagccga cccagacgag ccaagggatc 17880tttttggaat gctgctccgt cgtcaggctt tccgacgttt gggtggttga acagaagtca 17940ttatcgtacg gaatgccaag cactcccgag gggaaccctg tggttggcat gcacatacaa 18000atggacgaac ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct 18060cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag 18120gcgggaaacg acaatctgat catgagcgga gaattaaggg agtcacgtta tgacccccgc 18180cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag 18240ccactcagcc caagcttttt ggaaggctaa ggagaggaag ccggcgagaa ggagggggcg 18300ttttacgtgt cactgtcctg tcgtgttggc tgttgacacg aatcatttct tccgcgcgtg 18360ggaagaagaa gatgcacatt agcggcctga agtagagatg tcaatgggga attccccagc 18420ggggattaac tccccagacc cgtacccatg aacatagacc ggcccccatc cccgaacccg 18480aacccgacct cgggtacgaa aatcctccca tacccattcc cgaccgggta ctaaataccc 18540atgggtatcc atacccgacc cgattattca aaaattaatg ggctttttat ttgttaaccg 18600gcggacgcaa tgcttgggac tctaggtttt tttactttgt tgaccggctg gcggctgggc 18660tttttcctac aggcccaaag ttggtcggca gccactaggc cacacgtcac aggcagccca 18720caagtaaatg tcgttggatt gctggatggt ggaataaaaa tcctagatgc tagattgttc 18780tggttccggg tatttttctc catggctaat cgggtttggg tttagccctc ccaaacccga 18840acccgccata cccgatgggt aagggattta ttccaaatct atacccatgg ggatttgttt 18900taacccatac cttaacccta atagaggaat tccccacggg taatcgggtt tcggggccca 18960ttgacatctc tagactgaag gcgtccaact caaatcatta aaaagtgttg acgcacgcgc 19020tgatgcgccg gccgcacagc acaggctgca cagcccgttt aatcagcgat ggagccccgg 19080ccgtcagcca gccaggtccg gcgtccgggt ctgcgccctg cggcgtcact gctgtcgcca 19140ccgtctccga tggtcccaca tccatccagc gggccgcgcg tggtacaaaa ggctcttcct 19200cgccgtcagg tgcagctgcc caaacaccag acacagactc caccaccccg cttcgatctt 19260ctgttgcagc tgaaatctgt cagattctgc agttcattcc tcatggtccg tcctgtagaa 19320accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa 19380aactgtggaa ttgatcagcg ttggtgggaa agcgcgttac aagaaagccg ggcaattgct 19440gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgcgggcaac 19500gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg 19560cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag 19620catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa 19680agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga 19740atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac 19800tatgccggaa tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat 19860atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctgccaggtg 19920gtggccaatg gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact 19980ggacaaggca ctagcgggac tttgcaagtg gtgaatccgc acctctgcca accgggtgaa 20040ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg 20100cttcgcgtcg gcatccggtc agtggcagtg aagggccaac agttcctgat taaccacaaa 20160ccgttctact ttactggctt tggtcgtcat gaagatgcgg acttacgtgg caaaggattc 20220gataacgtgc tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac 20280cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc 20340gtggtgattg atgaaactgc tgctgtcggc tttaacctct ctttaggcat tggtttcgaa 20400gcgggcaaca agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa 20460gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg 20520atgtggagta ttgccaacga accggatacc cgtccgcaag tgcacgggaa tatttcgcca 20580ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgcgt caatgtaatg 20640ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt 20700tattacggat ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa 20760gaacttctgg cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg 20820gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt 20880gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag 20940gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag 21000aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc 21060tggactggca tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg aatcaacaac 21120tctcctggcg caccatcgtc ggctacagcc tcggtgacgt ggggcaacct agacttgtcc 21180atcttctgga ttggccaact taattaatgt atgaaataaa aggatgcaca catagtgaca 21240tgctaatcac tataatgtgg gcatcaaagt tgtgtgttat gtgtaattac tagttatctg 21300aataaaagag aaagagatca tccatatttc ttatcctaaa tgaatgtcac gtgtctttat 21360aattctttga tgaaccagat gcatttcatt aaccaaatcc atatacatat aaatattaat 21420catatataat taatatcaat tgggttagca aaacaaatct agtctaggtg tgttttgcga 21480attgcggccc catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga 21540ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca 21600acatggtgga gcacgacacg cttgtctact ccaaaaatat caaagataca gtctcagaag 21660accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc 21720attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca 21780aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc 21840ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt 21900cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc 21960actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag 22020ggtacccggg gatccaccat gtctccggag aggagaccag ttgagattag gccagctaca 22080gcagctgata tggccgcggt ttgtgatatc gttaaccatt acattgagac gtctacagtg 22140aactttagga cagagccaca aacaccacaa gagtggattg atgatctaga gaggttgcaa 22200gatagatacc cttggttggt tgctgaggtt gagggtgttg tggctggtat tgcttacgct 22260gggccctgga aggctaggaa cgcttacgat tggacagttg agagtactgt ttacgtgtca 22320cataggcatc aaaggttggg cctaggatcc acattgtaca cacatttgct taagtctatg 22380gaggcgcaag gttttaagtc tgtggttgct gttataggcc ttccaaacga tccatctgtt 22440aggttgcatg aggctttggg atacacagcc cggggtacat tgcgcgcagc tggatacaag 22500catggtggat ggcatgatgt tggtttttgg caaagggatt ttgagttgcc agctcctcca 22560aggccagtta ggccagttac ccagatctga gtcgacctgc aggcatgccg ctgaaatcac 22620cagtctctct ctacaaatct atctctctct ataataatgt gtgagtagtt cccagataag 22680ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat 22740gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc 22800agtgggtacc gagctcgaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt 22860ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc 22920gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc 22980gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga 23040agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt 23100gattgtaacg atgacagagc gttgctgcct gtgatcaaat atcatctccc tcgcagagat 23160ccgaattatc agccttctta ttcatttctc gcttaaccgt gacaggctgt cgatcttgag 23220aactatgccg acataatagg aaatcgctgg ataaagccgc tgaggaagct gagtggcgct 23280atttctttag aagtgaacgt tgacgatcgt cgaccgtacc ccgatgaatt aattcggacg 23340tacgttctga acacagctgg atacttactt gggcgattgt catacatgac atcaacaatg 23400tacccgtttg tgtaaccgtc tcttggaggt tcgtatgaca ctagtggttc ccctcagctt 23460gcgactagat gttgaggcct aacattttat tagagagcag gctagttgct tagatacatg 23520atcttcaggc cgttatctgt cagggcaagc gaaaattggc catttatgac gaccaatgcc 23580ccgcagaagc tcccatcttt gccgccatag acgccgcgcc ccccttttgg ggtgtagaac 23640atccttttgc cagatgtgga aaagaagttc gttgtcccat tgttggcaat gacgtagtag 23700ccggcgaaag tgcgagaccc atttgcgcta tatataagcc tacgatttcc gttgcgacta 23760ttgtcgtaat tggatgaact attatcgtag ttgctctcag agttgtcgta atttgatgga 23820ctattgtcgt aattgcttat ggagttgtcg tagttgcttg gagaaatgtc gtagttggat 23880ggggagtagt catagggaag acgagcttca tccactaaaa caattggcag gtcagcaagt 23940gcctgccccg atgccatcgc aagtacgagg cttagaacca ccttcaacag atcgcgcata 24000gtcttcccca gctctctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa 24060cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac gtagtgaaca 24120aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc 24180ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca 24240gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta 24300agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt 24360tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct 24420ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg 24480tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat 24540tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg 24600acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg 24660ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca 24720atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc 24780aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact 24840tcggcgatca ccgcttccct catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg 24900gtgtcggctt gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac 24960atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaacaggtc aatgccgccg 25020agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct 25080aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg 25140tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga 25200tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca 25260tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc 25320acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga 25380acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatc 25440ttcatatgac gcctaacgcc tggcacagcg gatcgcaaac ctggcgcggc ttttggcaca 25500aaaggcgtga caggtttgcg aatccgttgc tgccacttgt taaccctttt gccagatttg 25560gtaactataa tttatgttag aggcgaagtc ttgggtaaaa actggcctaa aattgctggg 25620gatttcagga aagtaaacat caccttccgg ctcgatgtct attgtagata tatgtagtgt 25680atctacttga tcgggggatc tgctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 25740gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 25800aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 25860cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 25920gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 25980caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 26040agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 26100aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 26160gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 26220tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 26280cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 26340ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 26400cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 26460atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 26520agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 26580gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 26640gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 26700tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 26760agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 26820gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 26880aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 26940aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 27000ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 27060gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 27120aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 27180ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 27240tgctgcaggg gggggggggg ggggggactt ccattgttca ttccacggac aaaaacagag 27300aaaggaaacg acagaggcca aaaagcctcg ctttcagcac ctgtcgtttc ctttcttttc 27360agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa cgccttaaac 27420cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac ctgtcggatc 27480accggaaagg acccgtaaag tgataatgat tatcatctac atatcacaac gtgcgtggag 27540gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat tgatctgcat 27600caacttaacg taaaaacaac ttcagacaat acaaatcagc gacactgaat acggggcaac 27660ctcatgtccc cccccccccc ccccctgcag gcatcgtggt gtcacgctcg tcgtttggta 27720tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 27780gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 27840tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 27900gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 27960gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt 28020taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 28080tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 28140ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 28200taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 28260tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 28320aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 28380ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat 28440tcggagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat 28500aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc 28560gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca 28620ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag 28680tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg 28740cagagcatta cgctgacttg acgggacggc ggctttgttg aataaatcga acttttgctg 28800agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa 28860agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt ggctccctca 28920ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca ccttcttcac 28980gaggcagacc tcagcgccag aaggccgcca gagaggccga gcgcggccgt gaggcttgga 29040cgctagggca gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga 29100aagggggagg ggatgttgtc tacatggctc tgctgtagtg agtgggttgc gctccggcag 29160cggtcctgat caatcgtcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct 29220tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc 29280ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt 29340gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt 29400gaagtagctg attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca 29460gaggaagcga agctgcgcgt cggccgtttc catctgcggt

gcgcccggtc gcgtgccggc 29520atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc 29580gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc 29640cagcatggct tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag 29700cgccggctgc tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc 29760gacctcgttc aacaggtcca gggcggcacg gatcactgta ttcggctgca actttgtcat 29820gcttgacact ttatcactga taaacataat atgtccacca acttatcagt gataaagaat 29880ccgcgcgttc aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacata 29940cccctgatcg taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg 30000ccggtgctgc cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat 30060ggcattctgc tggcgctgta tgcgttggtg caatttgcct gcgcacctgt gctgggcgcg 30120ctgtcggatc gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg cgccactgtc 30180gactacgcca tcatggcgac agcgcctttc ctttgggttc tctatatcgg gcggatcgtg 30240gccggcatca ccggggcgac tggggcggta gccggcgctt atattgccga tatcactgat 30300ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt cgggatggtc 30360gcgggacctg tgctcggtgg gctgatgggc ggtttctccc cccacgctcc gttcttcgcc 30420gcggcagcct tgaacggcct caatttcctg acgggctgtt tccttttgcc ggagtcgcac 30480aaaggcgaac gccggccgtt acgccgggag gctctcaacc cgctcgcttc gttccggtgg 30540gcccggggca tgaccgtcgt cgccgccctg atggcggtct tcttcatcat gcaacttgtc 30600ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg 30660accacgatcg gcatttcgct tgccgcattt ggcattctgc attcactcgc ccaggcaatg 30720atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg cactcatgct cggaatgatt 30780gccgacggca caggctacat cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg 30840atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc 30900aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct cactggcggc gctcaccagc 30960ctgacctcga tcgtcggacc cctcctcttc acggcgatct atgcggcttc tataacaacg 31020tggaacgggt gggcatggat tgcaggcgct gccctctact tgctctgcct gccggcgctg 31080cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc gctgatcgtg gaaacgatag 31140gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat acgcgcccta ggagtgcggt 31200tggaacgttg gcccagccag atactcccga tcacgagcag gacgccgatg atttgaagcg 31260cactcagcgt ctgatccaag aacaaccatc ctagcaacac ggcggtcccc gggctgagaa 31320agcccagtaa ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt 31380aggttaaacc cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag 31440gcatcgggat tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg 31500ccagttgcca ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca 31560cggttccgaa cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta 31620gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca 31680ggaccgccat caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca 31740gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa 31800acaacaagct ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc 31860agattcccgt tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc 31920gcagcagcat accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca 31980gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga 32040tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct 32100ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca 32160gggccgacaa tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct 32220gagccttaat cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc 32280cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg 32340aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta 32400gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg 32460caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga 32520tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa 32580atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt 32640cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg 32700ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag 32760gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc 32820ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta 32880gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg 32940ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc 33000cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg 33060cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca 33120cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag 33180caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg 33240cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc 33300ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg 33360agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac 33420ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc 33480ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg 33540attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc 33600ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc 33660ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac 33720gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca 33780cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccactcttc 33840attaaccgct atatcgaaaa ttgcttgcgg cttgttagaa ttgccatgac gtacctcggt 33900gtcacgggta agattaccga taaactggaa ctgattatgg ctcatatcga aagtctcctt 33960gagaaaggag actctagttt agctaaacat tggttccgct gtcaagaact ttagcggcta 34020aaattttgcg ggccgcgacc aaaggtgcga ggggcggctt ccgctgtgta caaccagata 34080tttttcacca acatccttcg tctgctcgat gagcggggca tgacgaaaca tgagctgtcg 34140gagagggcag gggtttcaat ttcgttttta tcagacttaa ccaacggtaa ggccaacccc 34200tcgttgaagg tgatggaggc cattgccgac gccctggaaa ctcccctacc tcttctcctg 34260gagtccaccg accttgaccg cgaggcactc gcggagattg cgggtcatcc tttcaagagc 34320agcgtgccgc ccggatacga acgcatcagt gtggttttgc cgtcacataa ggcgtttatc 34380gtaaagaaat ggggcgacga cacccgaaaa aagctgcgtg gaaggctctg acgccaaggg 34440ttagggcttg cacttccttc tttagccgct aaaacggccc cttctctgcg ggccgtcggc 34500tcgcgcatca tatcgacatc ctcaacggaa gccgtgccgc gaatggcatc gggcgggtgc 34560gctttgacag ttgttttcta tcagaacccc tacgtcgtgc ggttcgatta gctgtttgtc 34620ttgcaggcta aacactttcg gtatatcgtt tgcctgtgcg ataatgttgc taatgatttg 34680ttgcgtaggg gttactgaaa agtgagcggg aaagaagagt ttcagaccat caaggagcgg 34740gccaagcgca agctggaacg cgacatgggt gcggacctgt tggccgcgct caacgacccg 34800aaaaccgttg aagtcatgct caacgcggac ggcaaggtgt ggcacgaacg ccttggcgag 34860ccgatgcggt acatctgcga catgcggccc agccagtcgc aggcgattat agaaacggtg 34920gccggattcc acggcaaaga ggtcacgcgg cattcgccca tcctggaagg cgagttcccc 34980ttggatggca gccgctttgc cggccaattg ccgccggtcg tggccgcgcc aacctttgcg 35040atccgcaagc gcgcggtcgc catcttcacg ctggaacagt acgtcgaggc gggcatcatg 35100acccgcgagc aatacgaggt cattaaaagc gccgtcgcgg cgcatcgaaa catcctcgtc 35160attggcggta ctggctcggg caagaccacg ctcgtcaacg cgatcatcaa tgaaatggtc 35220gccttcaacc cgtctgagcg cgtcgtcatc atcgaggaca ccggcgaaat ccagtgcgcc 35280gcagagaacg ccgtccaata ccacaccagc atcgacgtct cgatgacgct gctgctcaag 35340acaacgctgc gtatgcgccc cgaccgcatc ctggtcggtg aggtacgtgg ccccgaagcc 35400cttgatctgt tgatggcctg gaacaccggg catgaaggag gtgccgccac cctgcacgca 35460aacaacccca aagcgggcct gagccggctc gccatgctta tcagcatgca cccggattca 35520ccgaaaccca ttgagccgct gattggcgag gcggttcatg tggtcgtcca tatcgccagg 35580acccctagcg gccgtcgagt gcaagaaatt ctcgaagttc ttggttacga gaacggccag 35640tacatcacca aaaccctgta aggagtattt ccaatgacaa cggctgttcc gttccgtctg 35700accatgaatc gcggcatttt gttctacctt gccgtgttct tcgttctcgc tctcgcgtta 35760tccgcgcatc cggcgatggc ctcggaaggc accggcggca gcttgccata tgagagctgg 35820ctgacgaacc tgcgcaactc cgtaaccggc ccggtggcct tcgcgctgtc catcatcggc 35880atcgtcgtcg ccggcggcgt gctgatcttc ggcggcgaac tcaacgcctt cttccgaacc 35940ctgatcttcc tggttctggt gatggcgctg ctggtcggcg cgcagaacgt gatgagcacc 36000ttcttcggtc gtggtgccga aatcgcggcc ctcggcaacg gggcgctgca ccaggtgcaa 36060gtcgcggcgg cggatgccgt gcgtgcggta gcggctggac ggctcgccta atcatggctc 36120tgcgcacgat ccccatccgt cgcgcaggca accgagaaaa cctgttcatg ggtggtgatc 36180gtgaactggt gatgttctcg ggcctgatgg cgtttgcgct gattttcagc gcccaagagc 36240tgcgggccac cgtggtcggt ctgatcctgt ggttcggggc gctctatgcg ttccgaatca 36300tggcgaaggc cgatccgaag atgcggttcg tgtacctgcg tcaccgccgg tacaagccgt 36360attacccggc ccgctcgacc ccgttccgcg agaacaccaa tagccaaggg aagcaatacc 36420gatgatccaa gcaattgcga ttgcaatcgc gggcctcggc gcgcttctgt tgttcatcct 36480ctttgcccgc atccgcgcgg tcgatgccga actgaaactg aaaaagcatc gttccaagga 36540cgccggcctg gccgatctgc tcaactacgc cgctgtcgtc gatgacggcg taatcgtggg 36600caagaacggc agctttatgg ctgcctggct gtacaagggc gatgacaacg caagcagcac 36660cgaccagcag cgcgaagtag tgtccgcccg catcaaccag gccctcgcgg gcctgggaag 36720tgggtggatg atccatgtgg acgccgtgcg gcgtcctgct ccgaactacg cggagcgggg 36780cctgtcggcg ttccctgacc gtctgacggc agcgattgaa gaagagcgct cggtcttgcc 36840ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat 36900ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg 36960gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt 37020cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt 37080cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca 37140gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca 37200gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt 37260cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat 37320cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag 37380gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg 37440cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt 37500ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa 37560aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgctgc ttccctgctg 37620ttttgtggaa tatctaccga ctggaaacag gcaaatgcag gaaattactg aactgagggg 37680acaggcgaga gacgatgcca aagagctaca ccgacgagct ggccgagtgg gttgaatccc 37740gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc gttcctggcg gtgagggcgg 37800atgtcgaggc ggcgttagcg tccggctatg cgctcgtcac catttgggag cacatgcggg 37860aaacggggaa ggtcaagttc tcctacgaga cgttccgctc gcacgccagg cggcacatca 37920aggccaagcc cgccgatgtg cccgcaccgc aggccaaggc tgcggaaccc gcgccggcac 37980ccaagacgcc ggagccacgg cggccgaagc aggggggcaa ggctgaaaag ccggcccccg 38040ctgcggcccc gaccggcttc accttcaacc caacaccgga caaaaaggat ctactgtaat 38100ggcgaaaatt cacatggttt tgcagggcaa gggcggggtc ggcaagtcgg ccatcgccgc 38160gatcattgcg cagtacaaga tggacaaggg gcagacaccc ttgtgcatcg acaccgaccc 38220ggtgaacgcg acgttcgagg gctacaaggc cctgaacgtc cgccggctga acatcatggc 38280cggcgacgaa attaactcgc gcaacttcga caccctggtc gagctgattg cgccgaccaa 38340ggatgacgtg gtgatcgaca acggtgccag ctcgttcgtg cctctgtcgc attacctcat 38400cagcaaccag gtgccggctc tgctgcaaga aatggggcat gagctggtca tccataccgt 38460cgtcaccggc ggccaggctc tcctggacac ggtgagcggc ttcgcccagc tcgccagcca 38520gttcccggcc gaagcgcttt tcgtggtctg gctgaacccg tattgggggc ctatcgagca 38580tgagggcaag agctttgagc agatgaaggc gtacacggcc aacaaggccc gcgtgtcgtc 38640catcatccag attccggccc tcaaggaaga aacctacggc cgcgatttca gcgacatgct 38700gcaagagcgg ctgacgttcg accaggcgct ggccgatgaa tcgctcacga tcatgacgcg 38760gcaacgcctc aagatcgtgc ggcgcggcct gtttgaacag ctcgacgcgg cggccgtgct 38820atgagcgacc agattgaaga gctgatccgg gagattgcgg ccaagcacgg catcgccgtc 38880ggccgcgacg acccggtgct gatcctgcat accatcaacg cccggctcat ggccgacagt 38940gcggccaagc aagaggaaat ccttgccgcg ttcaaggaag agctggaagg gatcgcccat 39000cgttggggcg aggacgccaa ggccaaagcg gagcggatgc tgaacgcggc cctggcggcc 39060agcaaggacg caatggcgaa ggtaatgaag gacagcgccg cgcaggcggc cgaagcgatc 39120cgcagggaaa tcgacgacgg ccttggccgc cagctcgcgg ccaaggtcgc ggacgcgcgg 39180cgcgtggcga tgatgaacat gatcgccggc ggcatggtgt tgttcgcggc cgccctggtg 39240gtgtgggcct cgttatgaat cgcagaggcg cagatgaaaa agcccggcgt tgccgggctt 39300tgtttttgcg ttagctgggc ttgtttgaca ggcccaagct ctgactgcgc ccgcgctcgc 39360gctcctgggc ctgtttcttc tcctgctcct gcttgcgcat cagggcctgg tgccgtcggg 39420ctgcttcacg catcgaatcc cagtcgccgg ccagctcggg atgctccgcg cgcatcttgc 39480gcgtcgccag ttcctcgatc ttgggcgcgt gaatgcccat gccttccttg atttcgcgca 39540ccatgtccag ccgcgtgtgc agggtctgca agcgggcttg ctgttgggcc tgctgctgct 39600gccaggcggc ctttgtacgc ggcagggaca gcaagccggg ggcattggac tgtagctgct 39660gcaaacgcgc ctgctgacgg tctacgagct gttctaggcg gtcctcgatg cgctccacct 39720ggtcatgctt tgcctgcacg tagagcgcaa gggtctgctg gtaggtctgc tcgatgggcg 39780cggattctaa gagggcctgc tgttccgtct cggcctcctg ggccgcctgt agcaaatcct 39840cgccgctgtt gccgctggac tgctttactg ccggggactg ctgttgccct gctcgcgccg 39900tcgtcgcagt tcggcttgcc cccactcgat tgactgcttc atttcgagcc gcagcgatgc 39960gatctcggat tgcgtcaacg gacggggcag cgcggaggtg tccggcttct ccttgggtga 40020gtcggtcgat gccatagcca aaggtttcct tccaaaatgc gtccattgct ggaccgtgtt 40080tctcattgat gcccgcaagc atcttcggct tgaccgccag gtcaagcgcg ccttcatggg 40140cggtcatgac ggacgccgcc atgaccttgc cgccgttgtt ctcgatgtag ccgcgtaatg 40200aggcaatggt gccgcccatc gtcagcgtgt catcgacaac gatgtacttc tggccgggga 40260tcacctcccc ctcgaaagtc gggttgaacg ccaggcgatg atctgaaccg gctccggttc 40320gggcgacctt ctcccgctgc acaatgtccg tttcgacctc aaggccaagg cggtcggcca 40380gaacgaccgc catcatggcc ggaatcttgt tgttccccgc cgcctcgacg gcgaggactg 40440gaacgatgcg gggcttgtcg tcgccgatca gcgtcttgag ctgggcaaca gtgtcgtccg 40500aaatcaggcg ctcgaccaaa ttaagcgccg cttccgcgtc gccctgcttc gcagcctggt 40560attcaggctc gttggtcaaa gaaccaaggt cgccgttgcg aaccaccttc gggaagtctc 40620cccacggtgc gcgctcggct ctgctgtagc tgctcaagac gcctcccttt ttagccgcta 40680aaactctaac gagtgcgccc gcgactcaac ttgacgcttt cggcacttac ctgtgccttg 40740ccacttgcgt cataggtgat gcttttcgca ctcccgattt caggtacttt atcgaaatct 40800gaccgggcgt gcattacaaa gttcttcccc acctgttggt aaatgctgcc gctatctgcg 40860tggacgatgc tgccgtcgtg gcgctgcgac ttatcggcct tttgggccat atagatgttg 40920taaatgccag gtttcagggc cccggcttta tctaccttct ggttcgtcca tgcgccttgg 40980ttctcggtct ggacaattct ttgcccattc atgaccagga ggcggtgttt cattgggtga 41040ctcctgacgg ttgcctctgg tgttaaacgt gtcctggtcg cttgccggct aaaaaaaagc 41100cgacctcggc agttcgaggc cggctttccc tagagccggg cgcgtcaagg ttgttccatc 41160tattttagtg aactgcgttc gatttatcag ttactttcct cccgctttgt gtttcctccc 41220actcgtttcc gcgtctagcc gacccctcaa catagcggcc tcttcttggg ctgcctttgc 41280ctcttgccgc gcttcgtcac gctcggcttg caccgtcgta aagcgctcgg cctgcctggc 41340cgcctcttgc gccgccaact tcctttgctc ctggtgggcc tcggcgtcgg cctgcgcctt 41400cgctttcacc gctgccaact ccgtgcgcaa actctccgct tcgcgcctgg tggcgtcgcg 41460ctcgccgcga agcgcctgca tttcctggtt ggccgcgtcc agggtcttgc ggctctcttc 41520tttgaatgcg cgggcgtcct ggtgagcgta gtccagctcg gcgcgcagct cctgcgctcg 41580acgctccacc tcgtcggccc gctgcgtcgc cagcgcggcc cgctgctcgg ctcctgccag 41640ggcggtgcgt gcttcggcca gggcttgccg ctggcgtgcg gccagctcgg ccgcctcggc 41700ggcctgctgc tctagcaatg taacgcgcgc ctgggcttct tccagctcgc gggcctgcgc 41760ctcgaaggcg tcggccagct ccccgcgcac ggcttccaac tcgttgcgct cacgatccca 41820gccggcttgc gctgcctgca acgattcatt ggcaagggcc tgggcggctt gccagagggc 41880ggccacggcc tggttgccgg cctgctgcac cgcgtccggc acctggactg ccagcggggc 41940ggcctgcgcc gtgcgctggc gtcgccattc gcgcatgccg gcgctggcgt cgttcatgtt 42000gacgcgggcg gccttacgca ctgcatccac ggtcgggaag ttctcccggt cgccttgctc 42060gaacagctcg tccgcagccg caaaaatgcg gtcgcgcgtc tctttgttca gttccatgtt 42120ggctccggta attggtaaga ataataatac tcttacctac cttatcagcg caagagttta 42180gctgaacagt tctcgactta acggcaggtt ttttagcggc tgaagggcag gcaaaaaaag 42240ccccgcacgg tcggcggggg caaagggtca gcgggaaggg gattagcggg cgtcgggctt 42300cttcatgcgt cggggccgcg cttcttggga tggagcacga cgaagcgcgc acgcgcatcg 42360tcctcggccc tatcggcccg cgtcgcggtc aggaacttgt cgcgcgctag gtcctccctg 42420gtgggcacca ggggcatgaa ctcggcctgc tcgatgtagg tccactccat gaccgcatcg 42480cagtcgaggc cgcgttcctt caccgtctct tgcaggtcgc ggtacgcccg ctcgttgagc 42540ggctggtaac gggccaattg gtcgtaaatg gctgtcggcc atgagcggcc tttcctgttg 42600agccagcagc cgacgacgaa gccggcaatg caggcccctg gcacaaccag gccgacgccg 42660ggggcagggg atggcagcag ctcgccaacc aggaaccccg ccgcgatgat gccgatgccg 42720gtcaaccagc ccttgaaact atccggcccc gaaacacccc tgcgcattgc ctggatgctg 42780cgccggatag cttgcaacat caggagccgt ttcttttgtt cgtcagtcat ggtccgccct 42840caccagttgt tcgtatcggt gtcggacgaa ctgaaatcgc aagagctgcc ggtatcggtc 42900cagccgctgt ccgtgtcgct gctgccgaag cacggcgagg ggtccgcgaa cgccgcagac 42960ggcgtatccg gccgcagcgc atcgcccagc atggccccgg tcagcgagcc gccggccagg 43020tagcccagca tggtgctgtt ggtcgccccg gccaccaggg ccgacgtgac gaaatcgccg 43080tcattccctc tggattgttc gctgctcggc ggggcagtgc gccgcgccgg cggcgtcgtg 43140gatggctcgg gttggctggc ctgcgacggc cggcgaaagg tgcgcagcag ctcgttatcg 43200accggctgcg gcgtcggggc cgccgccttg cgctgcggtc ggtgttcctt cttcggctcg 43260cgcagcttga acagcatgat cgcggaaacc agcagcaacg ccgcgcctac gcctcccgcg 43320atgtagaaca gcatcggatt cattcttcgg tcctccttgt agcggaaccg ttgtctgtgc 43380ggcgcgggtg gcccgcgccg ctgtctttgg ggatcagccc tcgatgagcg cgaccagttt 43440cacgtcggca aggttcgcct cgaactcctg gccgtcgtcc tcgtacttca accaggcata 43500gccttccgcc ggcggccgac ggttgaggat aaggcgggca gggcgctcgt cgtgctcgac 43560ctggacgatg gcctttttca gcttgtccgg gtccggctcc ttcgcgccct tttccttggc 43620gtccttaccg tcctggtcgc cgtcctcgcc gtcctggccg tcgccggcct ccgcgtcacg 43680ctcggcatca gtctggccgt tgaaggcatc gacggtgttg ggatcgcggc ccttctcgtc 43740caggaactcg cgcagcagct tgaccgtgcc gcgcgtgatt tcctgggtgt cgtcgtcaag 43800ccacgcctcg acttcctccg ggcgcttctt gaaggccgtc accagctcgt tcaccacggt 43860cacgtcgcgc acgcggccgg tgttgaacgc atcggcgatc ttctccggca ggtccagcag 43920cgtgacgtgc tgggtgatga acgccggcga cttgccgatt tccttggcga tatcgccttt 43980cttcttgccc ttcgccagct cgcggccaat gaagtcggca atttcgcgcg gggtcagctc 44040gttgcgttgc aggttctcga taacctggtc ggcttcgttg tagtcgttgt cgatgaacgc 44100cgggatggac ttcttgccgg cccacttcga gccacggtag cggcgggcgc cgtgattgat 44160gatatagcgg cccggctgct cctggttctc gcgcaccgaa atgggtgact tcaccccgcg 44220ctctttgatc gtggcaccga tttccgcgat gctctccggg gaaaagccgg ggttgtcggc 44280cgtccgcggc tgatgcggat cttcgtcgat caggtccagg tccagctcga tagggccgga 44340accgccctga gacgccgcag gagcgtccag gaggctcgac aggtcgccga tgctatccaa 44400ccccaggccg gacggctgcg ccgcgcctgc ggcttcctga gcggccgcag cggtgttttt 44460cttggtggtc ttggcttgag ccgcagtcat tgggaaatct ccatcttcgt gaacacgtaa 44520tcagccaggg cgcgaacctc tttcgatgcc ttgcgcgcgg

ccgttttctt gatcttccag 44580accggcacac cggatgcgag ggcatcggcg atgctgctgc gcaggccaac ggtggccgga 44640atcatcatct tggggtacgc ggccagcagc tcggcttggt ggcgcgcgtg gcgcggattc 44700cgcgcatcga ccttgctggg caccatgcca aggaattgca gcttggcgtt cttctggcgc 44760acgttcgcaa tggtcgtgac catcttcttg atgccctgga tgctgtacgc ctcaagctcg 44820atgggggaca gcacatagtc ggccgcgaag agggcggccg ccaggccgac gccaagggtc 44880ggggccgtgt cgatcaggca cacgtcgaag ccttggttcg ccagggcctt gatgttcgcc 44940ccgaacagct cgcgggcgtc gtccagcgac agccgttcgg cgttcgccag taccgggttg 45000gactcgatga gggcgaggcg cgcggcctgg ccgtcgccgg ctgcgggtgc ggtttcggtc 45060cagccgccgg cagggacagc gccgaacagc ttgcttgcat gcaggccggt agcaaagtcc 45120ttgagcgtgt aggacgcatt gccctggggg tccaggtcga tcacggcaac ccgcaagccg 45180cgctcgaaaa agtcgaaggc aagatgcaca agggtcgaag tcttgccgac gccgcctttc 45240tggttggccg tgaccaaagt tttcatcgtt tggtttcctg ttttttcttg gcgtccgctt 45300cccacttccg gacgatgtac gcctgatgtt ccggcagaac cgccgttacc cgcgcgtacc 45360cctcgggcaa gttcttgtcc tcgaacgcgg cccacacgcg atgcaccgct tgcgacactg 45420cgcccctggt cagtcccagc gacgttgcga acgtcgcctg tggcttccca tcgactaaga 45480cgccccgcgc tatctcgatg gtctgctgcc ccacttccag cccctggatc gcctcctgga 45540actggctttc ggtaagccgt ttcttcatgg ataacaccca taatttgctc cgcgccttgg 45600ttgaacatag cggtgacagc cgccagcaca tgagagaagt ttagctaaac atttctcgca 45660cgtcaacacc tttagccgct aaaactcgtc cttggcgtaa caaaacaaaa gcccggaaac 45720cgggctttcg tctcttgccg cttatggctc tgcacccggc tccatcacca acaggtcgcg 45780cacgcgcttc actcggttgc ggatcgacac tgccagccca acaaagccgg ttgccgccgc 45840cgccaggatc gcgccgatga tgccggccac accggccatc gcccaccagg tcgccgcctt 45900ccggttccat tcctgctggt actgcttcgc aatgctggac ctcggctcac cataggctga 45960ccgctcgatg gcgtatgccg cttctcccct tggcgtaaaa cccagcgccg caggcggcat 46020tgccatgctg cccgccgctt tcccgaccac gacgcgcgca ccaggcttgc ggtccagacc 46080ttcggccacg gcgagctgcg caaggacata atcagccgcc gacttggctc cacgcgcctc 46140gatcagctct tgcactcgcg cgaaatcctt ggcctccacg gccgccatga atcgcgcacg 46200cggcgaaggc tccgcagggc cggcgtcgtg atcgccgccg agaatgccct tcaccaagtt 46260cgacgacacg aaaatcatgc tgacggctat caccatcatg cagacggatc gcacgaaccc 46320gctgaattga acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa 46380attgccggcc ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg 46440ccacccaggc cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc 46500tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc 46560ccgatcccgg caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg 46620gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg 46680agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt 46740aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc 46800gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa 46860tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc 46920ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag 46980gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga 47040ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc 47100gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa 47160gttttccgcg aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg 47220cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag 47280cccggtgagc gtcggaaagg cgctggaagc cccgtagcga cgcggagagg ggcgagacaa 47340gccaagggcg caggctcgat gcgcagcacg acatagccgg ttctcgcaag gacgagaatt 47400tccctgcggt gcccctcaag tgtcaatgaa agtttccaac gcgagccatt cgcgagagcc 47460ttgagtccac gctagatgag agctttgttg taggtggacc agttggtgat tttgaacttt 47520tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca 47580gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt 47640gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat 47700acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgac 477471248474DNAartificial sequencePHP16975 ZmCAS1BamProGUS/35SPAT 12tctagagctc gttcctcgag gaacggtacc tgcggggaag cttacaataa tgtgtgttgt 60taagtcttgt tgcctgtcat cgtctgactg actttcgtca taaatcccgg cctccgtaac 120ccagctttgg gcaagctcac ggatttgatc cggcggaacg ggaatatcga gatgccgggc 180tgaacgctgc agttccagct ttccctttcg ggacaggtac tccagctgat tgattatctg 240ctgaagggtc ttggttccac ctcctggcac aatgcgaatg attacttgag cgcgatcggg 300catccaattt tctcccgtca ggtgcgtggt caagtgctac aaggcacctt tcagtaacga 360gcgaccgtcg atccgtcgcc gggatacgga caaaatggag cgcagtagtc catcgagggc 420ggcgaaagcc tcgccaaaag caatacgttc atctcgcaca gcctccagat ccgatcgagg 480gtcttcggcg taggcagata gaagcatgga tacattgctt gagagtattc cgatggactg 540aagtatggct tccatctttt ctcgtgtgtc tgcatctatt tcgagaaagc ccccgatgcg 600gcgcaccgca acgcgaattg ccatactatc cgaaagtccc agcaggcgcg cttgatagga 660aaaggtttca tactcggccg atcgcagacg ggcactcacg accttgaacc cttcaacttt 720cagggatcga tgctggttga tggtagtctc actcgacgtg gctctggtgt gttttgacat 780agcttcctcc aaagaaagcg gaaggtctgg atactccagc acgaaatgtg cccgggtaga 840cggatggaag tctagccctg ctcaatatga aatcaacagt acatttacag tcaatactga 900atatacttgc tacatttgca attgtcttat aacgaatgtg aaataaaaat agtgtaacaa 960cgcttttact catcgataat cacaaaaaca tttatacgaa caaaaataca aatgcactcc 1020ggtttcacag gataggcggg atcagaatat gcaacttttg acgttttgtt ctttcaaagg 1080gggtgctggc aaaaccaccg cactcatggg cctttgcgct gctttggcaa atgacggtaa 1140acgagtggcc ctctttgatg ccgacgaaaa ccggcctctg acgcgatgga gagaaaacgc 1200cttacaaagc agtactggga tcctcgctgt gaagtctatt ccgccgacga aatgcccctt 1260cttgaagcag cctatgaaaa tgccgagctc gaaggatttg attatgcgtt ggccgatacg 1320cgtggcggct cgagcgagct caacaacaca atcatcgcta gctcaaacct gcttctgatc 1380cccaccatgc taacgccgct cgacatcgat gaggcactat ctacctaccg ctacgtcatc 1440gagctgctgt tgagtgaaaa tttggcaatt cctacagctg ttttgcgcca acgcgtcccg 1500gtcggccgat tgacaacatc gcaacgcagg atgtcagaga cgctagagag ccttccagtt 1560gtaccgtctc ccatgcatga aagagatgca tttgccgcga tgaaagaacg cggcatgttg 1620catcttacat tactaaacac gggaactgat ccgacgatgc gcctcataga gaggaatctt 1680cggattgcga tggaggaagt cgtggtcatt tcgaaactga tcagcaaaat cttggaggct 1740tgaagatggc aattcgcaag cccgcattgt cggtcggcga agcacggcgg cttgctggtg 1800ctcgacccga gatccaccat cccaacccga cacttgttcc ccagaagctg gacctccagc 1860acttgcctga aaaagccgac gagaaagacc agcaacgtga gcctctcgtc gccgatcaca 1920tttacagtcc cgatcgacaa cttaagctaa ctgtggatgc ccttagtcca cctccgtccc 1980cgaaaaagct ccaggttttt ctttcagcgc gaccgcccgc gcctcaagtg tcgaaaacat 2040atgacaacct cgttcggcaa tacagtccct cgaagtcgct acaaatgatt ttaaggcgcg 2100cgttggacga tttcgaaagc atgctggcag atggatcatt tcgcgtggcc ccgaaaagtt 2160atccgatccc ttcaactaca gaaaaatccg ttctcgttca gacctcacgc atgttcccgg 2220ttgcgttgct cgaggtcgct cgaagtcatt ttgatccgtt ggggttggag accgctcgag 2280ctttcggcca caagctggct accgccgcgc tcgcgtcatt ctttgctgga gagaagccat 2340cgagcaattg gtgaagaggg acctatcgga acccctcacc aaatattgag tgtaggtttg 2400aggccgctgg ccgcgtcctc agtcaccttt tgagccagat aattaagagc caaatgcaat 2460tggctcaggc tgccatcgtc cccccgtgcg aaacctgcac gtccgcgtca aagaaataac 2520cggcacctct tgctgttttt atcagttgag ggcttgacgg atccgcctca agtttgcggc 2580gcagccgcaa aatgagaaca tctatactcc tgtcgtaaac ctcctcgtcg cgtactcgac 2640tggcaatgag aagttgctcg cgcgatagaa cgtcgcgggg tttctctaaa aacgcgagga 2700gaagattgaa ctcacctgcc gtaagtttca cctcaccgcc agcttcggac atcaagcgac 2760gttgcctgag attaagtgtc cagtcagtaa aacaaaaaga ccgtcggtct ttggagcgga 2820caacgttggg gcgcacgcgc aaggcaaccc gaatgcgtgc aagaaactct ctcgtactaa 2880acggcttagc gataaaatca cttgctccta gctcgagtgc aacaacttta tccgtctcct 2940caaggcggtc gccactgata attatgattg gaatatcaga ctttgccgcc agatttcgaa 3000cgatctcaag cccatcttca cgacctaaat ttagatcaac aaccacgaca tcgaccgtcg 3060cggaagagag tactctagtg aactgggtgc tgtcggctac cgcggtcact ttgaaggcgt 3120ggatcgtaag gtattcgata ataagatgcc gcatagcgac atcgtcatcg ataagaagaa 3180cgtgtttcaa cggctcacct ttcaatctaa aatctgaacc cttgttcaca gcgcttgaga 3240aattttcacg tgaaggatgt acaatcatct ccagctaaat gggcagttcg tcagaattgc 3300ggctgaccgc ggatgacgaa aatgcgaacc aagtatttca attttatgac aaaagttctc 3360aatcgttgtt acaagtgaaa cgcttcgagg ttacagctac tattgattaa ggagatcgcc 3420tatggtctcg ccccggcgtc gtgcgtccgc cgcgagccag atctcgccta cttcataaac 3480gtcctcatag gcacggaatg gaatgatgac atcgatcgcc gtagagagca tgtcaatcag 3540tgtgcgatct tccaagctag caccttgggc gctacttttg acaagggaaa acagtttctt 3600gaatccttgg attggattcg cgccgtgtat tgttgaaatc gatcccggat gtcccgagac 3660gacttcactc agataagccc atgctgcatc gtcgcgcatc tcgccaagca atatccggtc 3720cggccgcata cgcagacttg cttggagcaa gtgctcggcg ctcacagcac ccagcccagc 3780accgttcttg gagtagagta gtctaacatg attatcgtgt ggaatgacga gttcgagcgt 3840atcttctatg gtgattagcc tttcctgggg ggggatggcg ctgatcaagg tcttgctcat 3900tgttgtcttg ccgcttccgg tagggccaca tagcaacatc gtcagtcggc tgacgacgca 3960tgcgtgcaga aacgcttcca aatccccgtt gtcaaaatgc tgaaggatag cttcatcatc 4020ctgattttgg cgtttccttc gtgtctgcca ctggttccac ctcgaagcat cataacggga 4080ggagacttct ttaagaccag aaacacgcga gcttggccgt cgaatggtca agctgacggt 4140gcccgaggga acggtcggcg gcagacagat ttgtagtcgt tcaccaccag gaagttcagt 4200ggcgcagagg gggttacgtg gtccgacatc ctgctttctc agcgcgcccg ctaaaatagc 4260gatatcttca agatcatcat aagagacggg caaaggcatc ttggtaaaaa tgccggcttg 4320gcgcacaaat gcctctccag gtcgattgat cgcaatttct tcagtcttcg ggtcatcgag 4380ccattccaaa atcggcttca gaagaaagcg tagttgcgga tccacttcca tttacaatgt 4440atcctatctc taagcggaaa tttgaattca ttaagagcgg cggttcctcc cccgcgtggc 4500gccgccagtc aggcggagct ggtaaacacc aaagaaatcg aggtcccgtg ctacgaaaat 4560ggaaacggtg tcaccctgat tcttcttcag ggttggcggt atgttgatgg ttgccttaag 4620ggctgtctca gttgtctgct caccgttatt ttgaaagctg ttgaagctca tcccgccacc 4680cgagctgccg gcgtaggtgc tagctgcctg gaaggcgcct tgaacaacac tcaagagcat 4740agctccgcta aaacgctgcc agaagtggct gtcgaccgag cccggcaatc ctgagcgacc 4800gagttcgtcc gcgcttggcg atgttaacga gatcatcgca tggtcaggtg tctcggcgcg 4860atcccacaac acaaaaacgc gcccatctcc ctgttgcaag ccacgctgta tttcgccaac 4920aacggtggtg ccacgatcaa gaagcacgat attgttcgtt gttccacgaa tatcctgagg 4980caagacacac tttacatagc ctgccaaatt tgtgtcgatt gcggtttgca agatgcacgg 5040aattattgtc ccttgcgtta ccataaaatc ggggtgcggc aagagcgtgg cgctgctggg 5100ctgcagctcg gtgggtttca tacgtatcga caaatcgttc tcgccggaca cttcgccatt 5160cggcaaggag ttgtcgtcac gcttgccttc ttgtcttcgg cccgtgtcgc cctgaatggc 5220gcgtttgctg accccttgat cgccgctgct atatgcaaaa atcggtgttt cttccggccg 5280tggctcatgc cgctccggtt cgcccctcgg cggtagagga gcagcaggct gaacagcctc 5340ttgaaccgct ggaggatccg gcggcacctc aatcggagct ggatgaaatg gcttggtgtt 5400tgttgcgatc aaagttgacg gcgatgcgtt ctcattcacc ttcttttggc gcccacctag 5460ccaaatgagg cttaatgata acgcgagaac gacacctccg acgatcaatt tctgagaccc 5520cgaaagacgc cggcgatgtt tgtcggagac cagggatcca gatgcatcaa cctcatgtgc 5580cgcttgctga ctatcgttat tcatcccttc gcccccttca ggacgcgttt cacatcgggc 5640ctcaccgtgc ccgtttgcgg cctttggcca acgggatcgt aagcggtgtt ccagatacat 5700agtactgtgt ggccatccct cagacgccaa cctcgggaaa ccgaagaaat ctcgacatcg 5760ctccctttaa ctgaatagtt ggcaacagct tccttgccat caggattgat ggtgtagatg 5820gagggtatgc gtacattgcc cggaaagtgg aataccgtcg taaatccatt gtcgaagact 5880tcgagtggca acagcgaacg atcgccttgg gcgacgtagt gccaattact gtccgccgca 5940ccaagggctg tgacaggctg atccaataaa ttctcagctt tccgttgata ttgtgcttcc 6000gcgtgtagtc tgtccacaac agccttctgt tgtgcctccc ttcgccgagc cgccgcatcg 6060tcggcggggt aggcgaattg gacgctgtaa tagagatcgg gctgctcttt atcgaggtgg 6120gacagagtct tggaacttat actgaaaaca taacggcgca tcccggagtc gcttgcggtt 6180agcacgatta ctggctgagg cgtgaggacc tggcttgcct tgaaaaatag ataatttccc 6240cgcggtaggg ctgctagatc tttgctattt gaaacggcaa ccgctgtcac cgtttcgttc 6300gtggcgaatg ttacgaccaa agtagctcca accgccgtcg agaggcgcac cacttgatcg 6360ggattgtaag ccaaataacg catgcgcgga tctagcttgc ccgccattgg agtgtcttca 6420gcctccgcac cagtcgcagc ggcaaataaa catgctaaaa tgaaaagtgc ttttctgatc 6480atggttcgct gtggcctacg tttgaaacgg tatcttccga tgtctgatag gaggtgacaa 6540ccagacctgc cgggttggtt agtctcaatc tgccgggcaa gctggtcacc ttttcgtagc 6600gaactgtcgc ggtccacgta ctcaccacag gcattttgcc gtcaacgacg agggtccttt 6660tatagcgaat ttgctgcgtg cttggagtta catcatttga agcgatgtgc tcgacctcca 6720ccctgccgcg tttgccaaga atgacttgag gcgaactggg attgggatag ttgaagaatt 6780gctggtaatc ctggcgcact gttggggcac tgaagttcga taccaggtcg taggcgtact 6840gagcggtgtc ggcatcataa ctctcgcgca ggcgaacgta ctcccacaat gaggcgttaa 6900cgacggcctc ctcttgagtt gcaggcaatc gcgagacaga cacctcgctg tcaacggtgc 6960cgtccggccg tatccataga tatacgggca caagcctgct caacggcacc attgtggcta 7020tagcgaacgc ttgagcaaca tttcccaaaa tcgcgatagc tgcgacagct gcaatgagtt 7080tggagagacg tcgcgccgat ttcgctcgcg cggtttgaaa ggcttctact tccttatagt 7140gctcggcaag gctttcgcgc gccactagca tggcatattc aggccccgtc atagcgtcca 7200cccgaattgc cgagctgaag atctgacgga gtaggctgcc atcgccccac attcagcggg 7260aagatcgggc ctttgcagct cgctaatgtg tcgtttgtct ggcagccgct caaagcgaca 7320actaggcaca gcaggcaata cttcatagaa ttctccattg aggcgaattt ttgcgcgacc 7380tagcctcgct caacctgagc gaagcgacgg tacaagctgc tggcagattg ggttgcgccg 7440ctccagtaac tgcctccaat gttgccggcg atcgccggca aagcgacaat gagcgcatcc 7500cctgtcagaa aaaacatatc gagttcgtaa agaccaatga tcttggccgc ggtcgtaccg 7560gcgaaggtga ttacaccaag cataagggtg agcgcagtcg cttcggttag gatgacgatc 7620gttgccacga ggtttaagag gagaagcaag agaccgtagg tgataagttg cccgatccac 7680ttagctgcga tgtcccgcgt gcgatcaaaa atatatccga cgaggatcag aggcccgatc 7740gcgagaagca ctttcgtgag aattccaacg gcgtcgtaaa ctccgaaggc agaccagagc 7800gtgccgtaaa ggacccactg tgccccttgg aaagcaagga tgtcctggtc gttcatcgga 7860ccgatttcgg atgcgatttt ctgaaaaacg gcctgggtca cggcgaacat tgtatccaac 7920tgtgccggaa cagtctgcag aggcaagccg gttacactaa actgctgaac aaagtttggg 7980accgtctttt cgaagatgga aaccacatag tcttggtagt tagcctgccc aacaattaga 8040gcaacaacga tggtgaccgt gatcacccga gtgataccgc tacgggtatc gacttcgccg 8100cgtatgacta aaataccctg aacaataatc caaagagtga cacaggcgat caatggcgca 8160ctcaccgcct cctggatagt ctcaagcatc gagtccaagc ctgtcgtgaa ggctacatcg 8220aagatcgtat gaatggccgt aaacggcgcc ggaatcgtga aattcatcga ttggacctga 8280acttgactgg tttgtcgcat aatgttggat aaaatgagct cgcattcggc gaggatgcgg 8340gcggatgaac aaatcgccca gccttagggg agggcaccaa agatgacagc ggtcttttga 8400tgctccttgc gttgagcggc cgcctcttcc gcctcgtgaa ggccggcctg cgcggtagtc 8460atcgttaata ggcttgtcgc ctgtacattt tgaatcattg cgtcatggat ctgcttgaga 8520agcaaaccat tggtcacggt tgcctgcatg atattgcgag atcgggaaag ctgagcagac 8580gtatcagcat tcgccgtcaa gcgtttgtcc atcgtttcca gattgtcagc cgcaatgcca 8640gcgctgtttg cggaaccggt gatctgcgat cgcaacaggt ccgcttcagc atcactaccc 8700acgactgcac gatctgtatc gctggtgatc gcacgtgccg tggtcgacat tggcattcgc 8760ggcgaaaaca tttcattgtc taggtccttc gtcgaaggat actgattttt ctggttgagc 8820gaagtcagta gtccagtaac gccgtaggcc gacgtcaaca tcgtaaccat cgctatagtc 8880tgagtgagat tctccgcagt cgcgagcgca gtcgcgagcg tctcagcctc cgttgccggg 8940tcgctaacaa caaactgcgc ccgcgcgggc tgaatatata gaaagctgca ggtcaaaact 9000gttgcaataa gttgcgtcgt cttcatcgtt tcctacctta tcaatcttct gcctcgtggt 9060gacgggccat gaattcgctg agccagccag atgagttgcc ttcttgtgcc tcgcgtagtc 9120gagttgcaaa gcgcaccgtg ttggcacgcc ccgaaagcac ggcgacatat tcacgcatat 9180cccgcagatc aaattcgcag atgacgcttc cactttctcg tttaagaaga aacttacggc 9240tgccgaccgt catgtcttca cggatcgcct gaaattcctt ttcggtacat ttcagtccat 9300cgacataagc cgatcgatct gcggttggtg atggatagaa aatcttcgtc atacattgcg 9360caaccaagct ggctcctagc ggcgattcca gaacatgctc tggttgctgc gttgccagta 9420ttagcatccc gttgtttttt cgaacggtca ggaggaattt gtcgacgaca gtcgaaaatt 9480tagggtttaa caaataggcg cgaaactcat cgcagctcat cacaaaacgg cggccgtcga 9540tcatggctcc aatccgatgc aggagatatg ctgcagcggg agcgcatact tcctcgtatt 9600cgagaagatg cgtcatgtcg aagccggtaa tcgacggatc taactttact tcgtcaactt 9660cgccgtcaaa tgcccagcca agcgcatggc cccggcacca gcgttggagc cgcgctcctg 9720cgccttcggc gggcccatgc aacaaaaatt cacgtaaccc cgcgattgaa cgcatttgtg 9780gatcaaacga gagctgacga tggataccac ggaccagacg gcggttctct tccggagaaa 9840tcccaccccg accatcactc tcgatgagag ccacgatcca ttcgcgcaga aaatcgtgtg 9900aggctgctgt gttttctagg ccacgcaacg gcgccaaccc gctgggtgtg cctctgtgaa 9960gtgccaaata tgttcctcct gtggcgcgaa ccagcaattc gccaccccgg tccttgtcaa 10020agaacacgac cgtacctgca cggtcgacca tgctctgttc gagcatggct agaacaaaca 10080tcatgagcgt cgtcttaccc ctcccgatag gcccgaatat tgccgtcatg ccaacatcgt 10140gctcatgcgg gatatagtcg aaaggcgttc cgccattggt acgaaatcgg gcaatcgcgt 10200tgccccagtg gcctgagctg gcgccctctg gaaagttttc gaaagagaca aaccctgcga 10260aattgcgtga agtgattgcg ccagggcgtg tgcgccactt aaaattcccc ggcaattggg 10320accaataggc cgcttccata ccaatacctt cttggacaac cacggcacct gcatccgcca 10380ttcgtgtccg agcccgcgcg cccctgtccc caagactatt gagatcgtct gcatagacgc 10440aaaggctcaa atgatgtgag cccataacga attcgttgct cgcaagtgcg tcctcagcct 10500cggataattt gccgatttga gtcacggctt tatcgccgga actcagcatc tggctcgatt 10560tgaggctaag tttcgcgtgc gcttgcgggc gagtcaggaa cgaaaaactc tgcgtgagaa 10620caagtggaaa atcgagggat agcagcgcgt tgagcatgcc cggccgtgtt tttgcagggt 10680attcgcgaaa cgaatagatg gatccaacgt aactgtcttt tggcgttctg atctcgagtc 10740ctcgcttgcc gcaaatgact ctgtcggtat aaatcgaagc gccgagtgag ccgctgacga 10800ccggaaccgg tgtgaaccga ccagtcatga tcaaccgtag cgcttcgcca atttcggtga 10860agagcacacc ctgcttctcg cggatgccaa gacgatgcag gccatacgct ttaagagagc 10920cagcgacaac atgccaaaga tcttccatgt tcctgatctg gcccgtgaga tcgttttccc 10980tttttccgct tagcttggtg aacctcctct ttaccttccc taaagccgcc tgtgggtaga 11040caatcaacgt aaggaagtgt tcattgcgga ggagttggcc ggagagcacg cgctgttcaa 11100aagcttcgtt caggctagcg gcgaaaacac tacggaagtg tcgcggcgcc gatgatggca 11160cgtcggcatg acgtacgagg tgagcatata ttgacacatg atcatcagcg atattgcgca 11220acagcgtgtt gaacgcacga caacgcgcat tgcgcatttc agtttcctca agctcgaatg 11280caacgccatc aattctcgca atggtcatga tcgatccgtc ttcaagaagg acgatatggt 11340cgctgaggtg gccaatataa gggagataga tctcaccgga tctttcggtc gttccactcg 11400cgccgagcat cacaccattc ctctccctcg tgggggaacc ctaattggat ttgggctaac 11460agtagcgccc ccccaaactg cactatcaat gcttcttccc gcggtccgca aaaatagcag 11520gacgacgctc gccgcattgt agtctcgctc cacgatgagc cgggctgcaa accataacgg 11580cacgagaacg acttcgtaga gcgggttctg aacgataacg atgacaaagc cggcgaacat 11640catgaataac cctgccaatg tcagtggcac cccaagaaac aatgcgggcc gtgtggctgc 11700gaggtaaagg gtcgattctt ccaaacgatc agccatcaac taccgccagt gagcgtttgg 11760ccgaggaagc tcgccccaaa catgataaca atgccgccga

cgacgccggc aaccagccca 11820agcgaagccc gcccgaacat ccaggagatc ccgatagcga caatgccgag aacagcgagt 11880gactggccga acggaccaag gataaacgtg catatattgt taaccattgt ggcggggtca 11940gtgccgccac ccgcagattg cgctgcggcg ggtccggatg aggaaatgct ccatgcaatt 12000gcaccgcaca agcttggggc gcagctcgat atcacgcgca tcatcgcatt cgagagcgag 12060aggcgattta gatgtaaacg gtatctctca aagcatcgca tcaatgcgca cctccttagt 12120ataagtcgaa taagacttga ttgtcgtctg cggatttgcc gttgtcctgg tgtggcggtg 12180gcggagcgat taaaccgcca gcgccatcct cctgcgagcg gcgctgatat gacccccaaa 12240catcccacgt ctcttcggat tttagcgcct cgtgatcgtc ttttggaggc tcgattaacg 12300cgggcaccag cgattgagca gctgtttcaa cttttcgcac gtagccgttt gcaaaaccgc 12360cgatgaaatt accggtgttg taagcggaga tcgcccgacg aagcgcaaat tgcttctcgt 12420caatcgtttc gccgcctgca taacgacttt tcagcatgtt tgcagcggca gataatgatg 12480tgcacgcctg gagcgcaccg tcaggtgtca gaccgagcat agaaaaattt cgagagttta 12540tttgcatgag gccaacatcc agcgaatgcc gtgcatcgag acggtgcctg acgacttggg 12600ttgcttggct gtgatcttgc cagtgaagcg tttcgccggt cgtgttgtca tgaatcgcta 12660aaggatcaaa gcgactctcc accttagcta tcgccgcaag cgtagatgtc gcaactgatg 12720gggcacactt gcgagcaaca tggtcaaact cagcagatga gagtggcgtg gcaaggctcg 12780acgaacagaa ggagaccatc aaggcaagag aaagcgaccc cgatctctta agcatacctt 12840atctccttag ctcgcaacta acaccgcctc tcccgttgga agaagtgcgt tgttttatgt 12900tgaagattat cgggagggtc ggttactcga aaattttcaa ttgcttcttt atgatttcaa 12960ttgaagcgag aaacctcgcc cggcgtcttg gaacgcaaca tggaccgaga accgcgcatc 13020catgactaag caaccggatc gacctattca ggccgcagtt ggtcaggtca ggctcagaac 13080gaaaatgctc ggcgaggtta cgctgtctgt aaacccattc gatgaacggg aagcttcctt 13140ccgattgctc ttggcaggaa tattggccca tgcctgcttg cgctttgcaa atgctcttat 13200cgcgttggta tcatatgcct tgtccgccag cagaaacgca ctctaagcga ttatttgtaa 13260aaatgtttcg gtcatgcggc ggtcatgggc ttgacccgct gtcagcgcaa gacggatcgg 13320tcaaccgtcg gcatcgacaa cagcgtgaat cttggtggtc aaaccgccac gggaacgtcc 13380catacagcca tcgtcttgat cccgctgttt cccgtcgccg catgttggtg gacgcggaca 13440caggaactgt caatcatgac gacattctat cgaaagcctt ggaaatcaca ctcagaatat 13500gatcccagac gtctgcctca cgccatcgta caaagcgatt gtagcaggtt gtacaggaac 13560cgtatcgatc aggaacgtct gcccagggcg ggcccgtccg gaagcgccac aagatgacat 13620tgatcacccg cgtcaacgcg cggcacgcga cgcggcttat ttgggaacaa aggactgaac 13680aacagtccat tcgaaatcgg tgacatcaaa gcggggacgg gttatcagtg gcctccaagt 13740caagcctcaa tgaatcaaaa tcagaccgat ttgcaaacct gatttatgag tgtgcggcct 13800aaatgatgaa atcgtccttc tagatcgcct ccgtggtgta gcaacacctc gcagtatcgc 13860cgtgctgacc ttggccaggg aattgactgg caagggtgct ttcacatgac cgctcttttg 13920gccgcgatag atgatttcgt tgctgctttg ggcacgtaga aggagagaag tcatatcgga 13980gaaattcctc ctggcgcgag agcctgctct atcgcgacgg catcccactg tcgggaacag 14040accggatcat tcacgaggcg aaagtcgtca acacatgcgt tataggcatc ttcccttgaa 14100ggatgatctt gttgctgcca atctggaggt gcggcagccg caggcagatg cgatctcagc 14160gcaacttgcg gcaaaacatc tcactcacct gaaaaccact agcgagtctc gcgatcagac 14220gaaggccttt tacttaacga cacaatatcc gatgtctgca tcacaggcgt cgctatccca 14280gtcaatacta aagcggtgca ggaactaaag attactgatg acttaggcgt gccacgaggc 14340ctgagacgac gcgcgtagac agttttttga aatcattatc aaagtgatgg cctccgctga 14400agcctatcac ctctgcgccg gtctgtcgga gagatgggca agcattatta cggtcttcgc 14460gcccgtacat gcattggacg attgcagggt caatggatct gagatcatcc agaggattgc 14520cgcccttacc ttccgtttcg agttggagcc agcccctaaa tgagacgaca tagtcgactt 14580gatgtgacaa tgccaagaga gagatttgct taacccgatt tttttgctca agcgtaagcc 14640tattgaagct tgccggcatg acgtccgcgc cgaaagaata tcctacaagt aaaacattct 14700gcacaccgaa atgcttggtg tagacatcga ttatgtgacc aagatcctta gcagtttcgc 14760ttggggaccg ctccgaccag aaataccgaa gtgaactgac gccaatgaca ggaatccctt 14820ccgtctgcag ataggtacca tcgatagatc tgctgcctcg cgcgtttcgg tgatgacggt 14880gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 14940gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 15000atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 15060agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 15120aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 15180ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 15240gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 15300aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 15360gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 15420ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 15480cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 15540cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 15600gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 15660cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 15720agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 15780ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 15840ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 15900gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 15960cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 16020attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 16080accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 16140ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 16200gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 16260agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 16320ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 16380ttgttgccat tgctgcaggg gggggggggg ggggggactt ccattgttca ttccacggac 16440aaaaacagag aaaggaaacg acagaggcca aaaagcctcg ctttcagcac ctgtcgtttc 16500ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa 16560cgccttaaac cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac 16620ctgtcggatc accggaaagg acccgtaaag tgataatgat tatcatctac atatcacaac 16680gtgcgtggag gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat 16740tgatctgcat caacttaacg taaaaacaac ttcagacaat acaaatcagc gacactgaat 16800acggggcaac ctcatgtccc cccccccccc ccccctgcag ggcatcgtgg tgtcacgctc 16860gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 16920ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 16980gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 17040gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 17100gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 17160tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 17220gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 17280agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 17340aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 17400ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 17460gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 17520agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 17580tcttcaagaa ttggtcgacg atcttgctgc gttcggatat tttcgtggag ttcccgccac 17640agacccggat tgaaggcgag atccagcaac tcgcgccaga tcatcctgtg acggaacttt 17700ggcgcgtgat gactggccag gacgtcggcc gaaagagcga caagcagatc acgcttttcg 17760acagcgtcgg atttgcgatc gaggattttt cggcgctgcg ctacgtccgc gaccgcgttg 17820agggatcaag ccacagcagc ccactcgacc ttctagccga cccagacgag ccaagggatc 17880tttttggaat gctgctccgt cgtcaggctt tccgacgttt gggtggttga acagaagtca 17940ttatcgtacg gaatgccaag cactcccgag gggaaccctg tggttggcat gcacatacaa 18000atggacgaac ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct 18060cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag 18120gcgggaaacg acaatctgat catgagcgga gaattaaggg agtcacgtta tgacccccgc 18180cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag 18240ccactcagcc caagcttgat atcgaattcc tgcagcccgg gggatccggg gcggaagatg 18300gcagggacgc ggattcaggg cggacgcgct tgccgagggc gcgggggacc acagcgtgcg 18360ttacggggac agggcgggca tcgcgaggac gggtgcggga gcggagccac atctggtggt 18420ggacgcctac tttgctctct tatagagtag taaagattcg tggaccaaac aacaccctag 18480cttgtacaaa tattcttagg cagttgctac tgatgagaga aaaataacat cactccactg 18540catttgcgtg atttattgaa cagatcacaa ttacatctat tcaaatttat ttacctgtac 18600gtgtccgatt tttaggggag gattttttta cggtattttt tttttaaaaa aataaattta 18660ggcaacaatt ttatagaatc gagtgcttta tctattatct tttacaaggc acacgcgtac 18720aataaggttt ggtcgttcgt gacttggata gtggttttgg ttgcaattcc gtaattcttg 18780gcataggata cagcccaacc cagaaaaaaa taatgttgcg gtcagttctg gctttgagat 18840tcggagtacc acgtggcgta aaggcaggcc gtgtcttaca gatgaataaa ggacctgggt 18900ctcacgtgat tggtttccag tttcgtgcat caagatgtgg aattttcaaa ctgccgtcgt 18960gtttgtttcg tcacataaaa gctttttgga aggctaagga gaggaagccg gcgagaagga 19020gggggcgttt tacgtgtcac tgtcctgtcg tgttggctgt tgacacgaat catttcttcc 19080gcgcgtggga agaagaagat gcacattagc ggcctgaagt agagatgtca atggggaatt 19140ccccagcggg gattaactcc ccagacccgt acccatgaac atagaccggc ccccatcccc 19200gaacccgaac ccgacctcgg gtacgaaaat cctcccatac ccattcccga ccgggtacta 19260aatacccatg ggtatccata cccgacccga ttattcaaaa attaatgggc tttttatttg 19320ttaaccggcg gacgcaatgc ttgggactct aggttttttt actttgttga ccggctggcg 19380gctgggcttt ttcctacagg cccaaagttg gtcggcagcc actaggccac acgtcacagg 19440cagcccacaa gtaaatgtcg ttggattgct ggatggtgga ataaaaatcc tagatgctag 19500attgttctgg ttccgggtat ttttctccat ggctaatcgg gtttgggttt agccctccca 19560aacccgaacc cgccataccc gatgggtaag ggatttattc caaatctata cccatgggga 19620tttgttttaa cccatacctt aaccctaata gaggaattcc ccacgggtaa tcgggtttcg 19680gggcccattg acatctctag actgaaggcg tccaactcaa atcattaaaa agtgttgacg 19740cacgcgctga tgcgccggcc gcacagcaca ggctgcacag cccgtttaat cagcgatgga 19800gccccggccg tcagccagcc aggtccggcg tccgggtctg cgccctgcgg cgtcactgct 19860gtcgccaccg tctccgatgg tcccacatcc atccagcggg ccgcgcgtgg tacaaaaggc 19920tcttcctcgc cgtcaggtgc agctgcccaa acaccagaca cagactccac caccccgctt 19980cgatcttctg ttgcagctga aatctgtcag attctgcagt tcattcctca tggtccgtcc 20040tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga 20100tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc gcgttacaag aaagccgggc 20160aattgctgtg ccaggcagtt ttaacgatca gttcgccgat gcagatattc gtaattatgc 20220gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa ggttgggcag gccagcgtat 20280cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt 20340gatggagcat cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc 20400cgggaaaagt gtacgtatca ccgtttgtgt gaacaacgaa ctgaactggc agactatccc 20460gccgggaatg gtgattaccg acgaaaacgg caagaaaaag cagtcttact tccatgattt 20520ctttaactat gccggaatcc atcgcagcgt aatgctctac accacgccga acacctgggt 20580ggacgatatc accgtggtga cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg 20640ccaggtggtg gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt 20700tgcaactgga caaggcacta gcgggacttt gcaagtggtg aatccgcacc tctgccaacc 20760gggtgaaggt tatctctatg aactgtgcgt cacagccaaa agccagacag agtgtgatat 20820ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag ggccaacagt tcctgattaa 20880ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa gatgcggact tacgtggcaa 20940aggattcgat aacgtgctga tggtgcacga ccacgcatta atggactgga ttggggccaa 21000ctcctaccgt acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca 21060tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg 21120tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac 21180tcagcaagcg cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag 21240cgtggtgatg tggagtattg ccaacgaacc ggatacccgt ccgcaagtgc acgggaatat 21300ttcgccactg gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa 21360tgtaatgttc tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct 21420gaaccgttat tacggatggt atgtccaaag cggcgatttg gaaacggcag agaaggtact 21480ggaaaaagaa cttctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata 21540cggcgtggat acgttagccg ggctgcactc aatgtacacc gacatgtgga gtgaagagta 21600tcagtgtgca tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg 21660tgaacaggta tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg 21720taacaagaaa gggatcttca ctcgcgaccg caaaccgaag tcggcggctt ttctgctgca 21780aaaacgctgg actggcatga acttcggtga aaaaccgcag cagggaggca aacaatgaat 21840caacaactct cctggcgcac catcgtcggc tacagcctcg gtgacgtggg gcaacctaga 21900cttgtccatc ttctggattg gccaacttaa ttaatgtatg aaataaaagg atgcacacat 21960agtgacatgc taatcactat aatgtgggca tcaaagttgt gtgttatgtg taattactag 22020ttatctgaat aaaagagaaa gagatcatcc atatttctta tcctaaatga atgtcacgtg 22080tctttataat tctttgatga accagatgca tttcattaac caaatccata tacatataaa 22140tattaatcat atataattaa tatcaattgg gttagcaaaa caaatctagt ctaggtgtgt 22200tttgcgaatt gcggccccat ggagtcaaag attcaaatag aggacctaac agaactcgcc 22260gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc 22320ttcgtcaaca tggtggagca cgacacgctt gtctactcca aaaatatcaa agatacagtc 22380tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc 22440ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc 22500tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac 22560agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca 22620accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca 22680caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag 22740aggacagggt acccggggat ccaccatgtc tccggagagg agaccagttg agattaggcc 22800agctacagca gctgatatgg ccgcggtttg tgatatcgtt aaccattaca ttgagacgtc 22860tacagtgaac tttaggacag agccacaaac accacaagag tggattgatg atctagagag 22920gttgcaagat agataccctt ggttggttgc tgaggttgag ggtgttgtgg ctggtattgc 22980ttacgctggg ccctggaagg ctaggaacgc ttacgattgg acagttgaga gtactgttta 23040cgtgtcacat aggcatcaaa ggttgggcct aggatccaca ttgtacacac atttgcttaa 23100gtctatggag gcgcaaggtt ttaagtctgt ggttgctgtt ataggccttc caaacgatcc 23160atctgttagg ttgcatgagg ctttgggata cacagcccgg ggtacattgc gcgcagctgg 23220atacaagcat ggtggatggc atgatgttgg tttttggcaa agggattttg agttgccagc 23280tcctccaagg ccagttaggc cagttaccca gatctgagtc gacctgcagg catgccgctg 23340aaatcaccag tctctctcta caaatctatc tctctctata ataatgtgtg agtagttccc 23400agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc 23460ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc 23520aaaatccagt gggtaccgag ctcgaattca gtacattaaa aacgtccgca atgtgttatt 23580aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca 23640gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg 23700ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct 23760attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta 23820gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg 23880cagagatccg aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga 23940tcttgagaac tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag 24000tggcgctatt tctttagaag tgaacgttga cgatcgtcga ccgtaccccg atgaattaat 24060tcggacgtac gttctgaaca cagctggata cttacttggg cgattgtcat acatgacatc 24120aacaatgtac ccgtttgtgt aaccgtctct tggaggttcg tatgacacta gtggttcccc 24180tcagcttgcg actagatgtt gaggcctaac attttattag agagcaggct agttgcttag 24240atacatgatc ttcaggccgt tatctgtcag ggcaagcgaa aattggccat ttatgacgac 24300caatgccccg cagaagctcc catctttgcc gccatagacg ccgcgccccc cttttggggt 24360gtagaacatc cttttgccag atgtggaaaa gaagttcgtt gtcccattgt tggcaatgac 24420gtagtagccg gcgaaagtgc gagacccatt tgcgctatat ataagcctac gatttccgtt 24480gcgactattg tcgtaattgg atgaactatt atcgtagttg ctctcagagt tgtcgtaatt 24540tgatggacta ttgtcgtaat tgcttatgga gttgtcgtag ttgcttggag aaatgtcgta 24600gttggatggg gagtagtcat agggaagacg agcttcatcc actaaaacaa ttggcaggtc 24660agcaagtgcc tgccccgatg ccatcgcaag tacgaggctt agaaccacct tcaacagatc 24720gcgcatagtc ttccccagct ctctaacgct tgagttaagc cgcgccgcga agcggcgtcg 24780gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta 24840gtgaacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt gtccaagata 24900agcctgccta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca 24960gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga 25020caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt 25080taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc 25140cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag 25200atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc 25260tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac 25320aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa 25380aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag 25440caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac 25500ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt 25560cgatacttcg gcgatcaccg cttccctcat gatgtttaac tcctgaatta agccgcgccg 25620cgaagcggtg tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta 25680ccagtacatc gctgtttcgt tcgagacttg aggtctagtt ttatacgtga acaggtcaat 25740gccgccgaga gtaaagccac attttgcgta caaattgcag gcaggtacat tgttcgtttg 25800tgtctctaat cgtatgccaa ggagctgtct gcttagtgcc cactttttcg caaattcgat 25860gagactgtgc gcgactcctt tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga 25920ggctagatcg ttccatgttg agttgagttc aatcttcccg acaagctctt ggtcgatgaa 25980tgcgccatag caagcagagt cttcatcaga gtcatcatcc gagatgtaat ccttccggta 26040ggggctcaca cttctggtag atagttcaaa gccttggtcg gataggtgca catcgaacac 26100ttcacgaaca atgaaatggt tctcagcatc caatgtttcc gccacctgct cagggatcac 26160cgaaatcttc atatgacgcc taacgcctgg cacagcggat cgcaaacctg gcgcggcttt 26220tggcacaaaa ggcgtgacag gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc 26280agatttggta actataattt atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat 26340tgctggggat ttcaggaaag taaacatcac cttccggctc gatgtctatt gtagatatat 26400gtagtgtatc tacttgatcg ggggatctgc tgcctcgcgc gtttcggtga tgacggtgaa 26460aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 26520agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 26580acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 26640ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 26700accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 26760tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 26820ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa

ggccaggaac cgtaaaaagg 26880ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 26940gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 27000gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 27060ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 27120tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 27180gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 27240tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 27300tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 27360tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 27420ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 27480ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 27540gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 27600aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 27660aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 27720cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 27780ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 27840cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 27900ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 27960ttgccattgc tgcagggggg gggggggggg gggacttcca ttgttcattc cacggacaaa 28020aacagagaaa ggaaacgaca gaggccaaaa agcctcgctt tcagcacctg tcgtttcctt 28080tcttttcaga gggtatttta aataaaaaca ttaagttatg acgaagaaga acggaaacgc 28140cttaaaccgg aaaattttca taaatagcga aaacccgcga ggtcgccgcc ccgtaacctg 28200tcggatcacc ggaaaggacc cgtaaagtga taatgattat catctacata tcacaacgtg 28260cgtggaggcc atcaaaccac gtcaaataat caattatgac gcaggtatcg tattaattga 28320tctgcatcaa cttaacgtaa aaacaacttc agacaataca aatcagcgac actgaatacg 28380gggcaacctc atgtcccccc cccccccccc cctgcaggca tcgtggtgtc acgctcgtcg 28440tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 28500atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 28560gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 28620tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 28680atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 28740agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 28800ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 28860tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 28920aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 28980tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 29040aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 29100accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt 29160caagaattcg gagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc 29220acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt 29280cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc 29340tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa 29400attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta 29460acactggcag agcattacgc tgacttgacg ggacggcggc tttgttgaat aaatcgaact 29520tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca 29580aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc 29640tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct 29700tcttcacgag gcagacctca gcgccagaag gccgccagag aggccgagcg cggccgtgag 29760gcttggacgc tagggcaggg catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg 29820cggtggaaag ggggagggga tgttgtctac atggctctgc tgtagtgagt gggttgcgct 29880ccggcagcgg tcctgatcaa tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga 29940gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg 30000gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt 30060caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc 30120caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg 30180cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg 30240tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg 30300cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat 30360tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc 30420agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt 30480ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact 30540ttgtcatgct tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat 30600aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc 30660caacataccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct 30720gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc 30780ccactatggc attctgctgg cgctgtatgc gttggtgcaa tttgcctgcg cacctgtgct 30840gggcgcgctg tcggatcgtt tcgggcggcg gccaatcttg ctcgtctcgc tggccggcgc 30900cactgtcgac tacgccatca tggcgacagc gcctttcctt tgggttctct atatcgggcg 30960gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat 31020cactgatggc gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg 31080gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt 31140cttcgccgcg gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga 31200gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt 31260ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca 31320acttgtcgga caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc gctttcactg 31380ggacgcgacc acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca 31440ggcaatgatc accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg 31500aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc 31560gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat 31620gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct 31680caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat 31740aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc 31800ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa 31860acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga 31920gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt 31980tgaagcgcac tcagcgtctg atccaagaac aaccatccta gcaacacggc ggtccccggg 32040ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa 32100aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt 32160cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct 32220ccggccgcca gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg 32280gtcagcacgg ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca 32340ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata 32400gcccccagga ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg 32460accatcagcg gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc 32520gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc 32580atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc 32640aacgcccgca gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg 32700ccggacagat gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc 32760ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg 32820aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct 32880ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt 32940cgaatctgag ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag 33000agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt 33060gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa 33120ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc 33180tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg 33240aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg 33300agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata 33360tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct 33420ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg 33480attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca 33540ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa 33600gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca 33660cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct 33720tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct 33780tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt 33840ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt 33900gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg 33960tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac 34020ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg 34080cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg 34140agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt 34200cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa 34260acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc 34320cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa 34380tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc 34440cctgcacgaa taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc 34500caaaacactt gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc 34560actcttcatt aaccgctata tcgaaaattg cttgcggctt gttagaattg ccatgacgta 34620cctcggtgtc acgggtaaga ttaccgataa actggaactg attatggctc atatcgaaag 34680tctccttgag aaaggagact ctagtttagc taaacattgg ttccgctgtc aagaacttta 34740gcggctaaaa ttttgcgggc cgcgaccaaa ggtgcgaggg gcggcttccg ctgtgtacaa 34800ccagatattt ttcaccaaca tccttcgtct gctcgatgag cggggcatga cgaaacatga 34860gctgtcggag agggcagggg tttcaatttc gtttttatca gacttaacca acggtaaggc 34920caacccctcg ttgaaggtga tggaggccat tgccgacgcc ctggaaactc ccctacctct 34980tctcctggag tccaccgacc ttgaccgcga ggcactcgcg gagattgcgg gtcatccttt 35040caagagcagc gtgccgcccg gatacgaacg catcagtgtg gttttgccgt cacataaggc 35100gtttatcgta aagaaatggg gcgacgacac ccgaaaaaag ctgcgtggaa ggctctgacg 35160ccaagggtta gggcttgcac ttccttcttt agccgctaaa acggcccctt ctctgcgggc 35220cgtcggctcg cgcatcatat cgacatcctc aacggaagcc gtgccgcgaa tggcatcggg 35280cgggtgcgct ttgacagttg ttttctatca gaacccctac gtcgtgcggt tcgattagct 35340gtttgtcttg caggctaaac actttcggta tatcgtttgc ctgtgcgata atgttgctaa 35400tgatttgttg cgtaggggtt actgaaaagt gagcgggaaa gaagagtttc agaccatcaa 35460ggagcgggcc aagcgcaagc tggaacgcga catgggtgcg gacctgttgg ccgcgctcaa 35520cgacccgaaa accgttgaag tcatgctcaa cgcggacggc aaggtgtggc acgaacgcct 35580tggcgagccg atgcggtaca tctgcgacat gcggcccagc cagtcgcagg cgattataga 35640aacggtggcc ggattccacg gcaaagaggt cacgcggcat tcgcccatcc tggaaggcga 35700gttccccttg gatggcagcc gctttgccgg ccaattgccg ccggtcgtgg ccgcgccaac 35760ctttgcgatc cgcaagcgcg cggtcgccat cttcacgctg gaacagtacg tcgaggcggg 35820catcatgacc cgcgagcaat acgaggtcat taaaagcgcc gtcgcggcgc atcgaaacat 35880cctcgtcatt ggcggtactg gctcgggcaa gaccacgctc gtcaacgcga tcatcaatga 35940aatggtcgcc ttcaacccgt ctgagcgcgt cgtcatcatc gaggacaccg gcgaaatcca 36000gtgcgccgca gagaacgccg tccaatacca caccagcatc gacgtctcga tgacgctgct 36060gctcaagaca acgctgcgta tgcgccccga ccgcatcctg gtcggtgagg tacgtggccc 36120cgaagccctt gatctgttga tggcctggaa caccgggcat gaaggaggtg ccgccaccct 36180gcacgcaaac aaccccaaag cgggcctgag ccggctcgcc atgcttatca gcatgcaccc 36240ggattcaccg aaacccattg agccgctgat tggcgaggcg gttcatgtgg tcgtccatat 36300cgccaggacc cctagcggcc gtcgagtgca agaaattctc gaagttcttg gttacgagaa 36360cggccagtac atcaccaaaa ccctgtaagg agtatttcca atgacaacgg ctgttccgtt 36420ccgtctgacc atgaatcgcg gcattttgtt ctaccttgcc gtgttcttcg ttctcgctct 36480cgcgttatcc gcgcatccgg cgatggcctc ggaaggcacc ggcggcagct tgccatatga 36540gagctggctg acgaacctgc gcaactccgt aaccggcccg gtggccttcg cgctgtccat 36600catcggcatc gtcgtcgccg gcggcgtgct gatcttcggc ggcgaactca acgccttctt 36660ccgaaccctg atcttcctgg ttctggtgat ggcgctgctg gtcggcgcgc agaacgtgat 36720gagcaccttc ttcggtcgtg gtgccgaaat cgcggccctc ggcaacgggg cgctgcacca 36780ggtgcaagtc gcggcggcgg atgccgtgcg tgcggtagcg gctggacggc tcgcctaatc 36840atggctctgc gcacgatccc catccgtcgc gcaggcaacc gagaaaacct gttcatgggt 36900ggtgatcgtg aactggtgat gttctcgggc ctgatggcgt ttgcgctgat tttcagcgcc 36960caagagctgc gggccaccgt ggtcggtctg atcctgtggt tcggggcgct ctatgcgttc 37020cgaatcatgg cgaaggccga tccgaagatg cggttcgtgt acctgcgtca ccgccggtac 37080aagccgtatt acccggcccg ctcgaccccg ttccgcgaga acaccaatag ccaagggaag 37140caataccgat gatccaagca attgcgattg caatcgcggg cctcggcgcg cttctgttgt 37200tcatcctctt tgcccgcatc cgcgcggtcg atgccgaact gaaactgaaa aagcatcgtt 37260ccaaggacgc cggcctggcc gatctgctca actacgccgc tgtcgtcgat gacggcgtaa 37320tcgtgggcaa gaacggcagc tttatggctg cctggctgta caagggcgat gacaacgcaa 37380gcagcaccga ccagcagcgc gaagtagtgt ccgcccgcat caaccaggcc ctcgcgggcc 37440tgggaagtgg gtggatgatc catgtggacg ccgtgcggcg tcctgctccg aactacgcgg 37500agcggggcct gtcggcgttc cctgaccgtc tgacggcagc gattgaagaa gagcgctcgg 37560tcttgccttg ctcgtcggtg atgtacttca ccagctccgc gaagtcgctc ttcttgatgg 37620agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa 37680agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc 37740ttctcttcga tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc 37800gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg 37860cgggccagct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg 37920acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct 37980cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg 38040gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc 38100agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac 38160ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa 38220ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat 38280accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc 38340cctgctgttt tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac 38400tgaggggaca ggcgagagac gatgccaaag agctacaccg acgagctggc cgagtgggtt 38460gaatcccgcg cggccaagaa gcgccggcgt gatgaggctg cggttgcgtt cctggcggtg 38520agggcggatg tcgaggcggc gttagcgtcc ggctatgcgc tcgtcaccat ttgggagcac 38580atgcgggaaa cggggaaggt caagttctcc tacgagacgt tccgctcgca cgccaggcgg 38640cacatcaagg ccaagcccgc cgatgtgccc gcaccgcagg ccaaggctgc ggaacccgcg 38700ccggcaccca agacgccgga gccacggcgg ccgaagcagg ggggcaaggc tgaaaagccg 38760gcccccgctg cggccccgac cggcttcacc ttcaacccaa caccggacaa aaaggatcta 38820ctgtaatggc gaaaattcac atggttttgc agggcaaggg cggggtcggc aagtcggcca 38880tcgccgcgat cattgcgcag tacaagatgg acaaggggca gacacccttg tgcatcgaca 38940ccgacccggt gaacgcgacg ttcgagggct acaaggccct gaacgtccgc cggctgaaca 39000tcatggccgg cgacgaaatt aactcgcgca acttcgacac cctggtcgag ctgattgcgc 39060cgaccaagga tgacgtggtg atcgacaacg gtgccagctc gttcgtgcct ctgtcgcatt 39120acctcatcag caaccaggtg ccggctctgc tgcaagaaat ggggcatgag ctggtcatcc 39180ataccgtcgt caccggcggc caggctctcc tggacacggt gagcggcttc gcccagctcg 39240ccagccagtt cccggccgaa gcgcttttcg tggtctggct gaacccgtat tgggggccta 39300tcgagcatga gggcaagagc tttgagcaga tgaaggcgta cacggccaac aaggcccgcg 39360tgtcgtccat catccagatt ccggccctca aggaagaaac ctacggccgc gatttcagcg 39420acatgctgca agagcggctg acgttcgacc aggcgctggc cgatgaatcg ctcacgatca 39480tgacgcggca acgcctcaag atcgtgcggc gcggcctgtt tgaacagctc gacgcggcgg 39540ccgtgctatg agcgaccaga ttgaagagct gatccgggag attgcggcca agcacggcat 39600cgccgtcggc cgcgacgacc cggtgctgat cctgcatacc atcaacgccc ggctcatggc 39660cgacagtgcg gccaagcaag aggaaatcct tgccgcgttc aaggaagagc tggaagggat 39720cgcccatcgt tggggcgagg acgccaaggc caaagcggag cggatgctga acgcggccct 39780ggcggccagc aaggacgcaa tggcgaaggt aatgaaggac agcgccgcgc aggcggccga 39840agcgatccgc agggaaatcg acgacggcct tggccgccag ctcgcggcca aggtcgcgga 39900cgcgcggcgc gtggcgatga tgaacatgat cgccggcggc atggtgttgt tcgcggccgc 39960cctggtggtg tgggcctcgt tatgaatcgc agaggcgcag atgaaaaagc ccggcgttgc 40020cgggctttgt ttttgcgtta gctgggcttg tttgacaggc ccaagctctg actgcgcccg 40080cgctcgcgct cctgggcctg tttcttctcc tgctcctgct tgcgcatcag ggcctggtgc 40140cgtcgggctg cttcacgcat cgaatcccag tcgccggcca gctcgggatg ctccgcgcgc 40200atcttgcgcg tcgccagttc ctcgatcttg ggcgcgtgaa tgcccatgcc ttccttgatt 40260tcgcgcacca tgtccagccg cgtgtgcagg gtctgcaagc gggcttgctg ttgggcctgc 40320tgctgctgcc aggcggcctt tgtacgcggc agggacagca agccgggggc attggactgt 40380agctgctgca aacgcgcctg ctgacggtct acgagctgtt ctaggcggtc ctcgatgcgc 40440tccacctggt catgctttgc ctgcacgtag agcgcaaggg tctgctggta ggtctgctcg 40500atgggcgcgg attctaagag ggcctgctgt tccgtctcgg cctcctgggc cgcctgtagc 40560aaatcctcgc cgctgttgcc gctggactgc tttactgccg gggactgctg ttgccctgct 40620cgcgccgtcg tcgcagttcg gcttgccccc actcgattga ctgcttcatt tcgagccgca 40680gcgatgcgat ctcggattgc gtcaacggac ggggcagcgc ggaggtgtcc ggcttctcct 40740tgggtgagtc ggtcgatgcc atagccaaag gtttccttcc aaaatgcgtc cattgctgga 40800ccgtgtttct cattgatgcc cgcaagcatc ttcggcttga ccgccaggtc aagcgcgcct 40860tcatgggcgg tcatgacgga cgccgccatg accttgccgc cgttgttctc gatgtagccg 40920cgtaatgagg caatggtgcc gcccatcgtc agcgtgtcat cgacaacgat gtacttctgg 40980ccggggatca cctccccctc gaaagtcggg ttgaacgcca ggcgatgatc tgaaccggct 41040ccggttcggg cgaccttctc ccgctgcaca atgtccgttt cgacctcaag gccaaggcgg 41100tcggccagaa cgaccgccat catggccgga atcttgttgt tccccgccgc ctcgacggcg 41160aggactggaa cgatgcgggg cttgtcgtcg ccgatcagcg tcttgagctg ggcaacagtg 41220tcgtccgaaa tcaggcgctc gaccaaatta agcgccgctt ccgcgtcgcc ctgcttcgca 41280gcctggtatt caggctcgtt ggtcaaagaa ccaaggtcgc cgttgcgaac caccttcggg 41340aagtctcccc acggtgcgcg ctcggctctg ctgtagctgc tcaagacgcc tcccttttta 41400gccgctaaaa ctctaacgag tgcgcccgcg actcaacttg acgctttcgg cacttacctg 41460tgccttgcca cttgcgtcat aggtgatgct tttcgcactc ccgatttcag gtactttatc 41520gaaatctgac cgggcgtgca ttacaaagtt cttccccacc tgttggtaaa tgctgccgct 41580atctgcgtgg acgatgctgc cgtcgtggcg ctgcgactta tcggcctttt gggccatata 41640gatgttgtaa atgccaggtt tcagggcccc ggctttatct accttctggt tcgtccatgc 41700gccttggttc tcggtctgga caattctttg cccattcatg accaggaggc ggtgtttcat 41760tgggtgactc ctgacggttg cctctggtgt taaacgtgtc ctggtcgctt gccggctaaa 41820aaaaagccga cctcggcagt tcgaggccgg ctttccctag agccgggcgc gtcaaggttg 41880ttccatctat tttagtgaac tgcgttcgat ttatcagtta

ctttcctccc gctttgtgtt 41940tcctcccact cgtttccgcg tctagccgac ccctcaacat agcggcctct tcttgggctg 42000cctttgcctc ttgccgcgct tcgtcacgct cggcttgcac cgtcgtaaag cgctcggcct 42060gcctggccgc ctcttgcgcc gccaacttcc tttgctcctg gtgggcctcg gcgtcggcct 42120gcgccttcgc tttcaccgct gccaactccg tgcgcaaact ctccgcttcg cgcctggtgg 42180cgtcgcgctc gccgcgaagc gcctgcattt cctggttggc cgcgtccagg gtcttgcggc 42240tctcttcttt gaatgcgcgg gcgtcctggt gagcgtagtc cagctcggcg cgcagctcct 42300gcgctcgacg ctccacctcg tcggcccgct gcgtcgccag cgcggcccgc tgctcggctc 42360ctgccagggc ggtgcgtgct tcggccaggg cttgccgctg gcgtgcggcc agctcggccg 42420cctcggcggc ctgctgctct agcaatgtaa cgcgcgcctg ggcttcttcc agctcgcggg 42480cctgcgcctc gaaggcgtcg gccagctccc cgcgcacggc ttccaactcg ttgcgctcac 42540gatcccagcc ggcttgcgct gcctgcaacg attcattggc aagggcctgg gcggcttgcc 42600agagggcggc cacggcctgg ttgccggcct gctgcaccgc gtccggcacc tggactgcca 42660gcggggcggc ctgcgccgtg cgctggcgtc gccattcgcg catgccggcg ctggcgtcgt 42720tcatgttgac gcgggcggcc ttacgcactg catccacggt cgggaagttc tcccggtcgc 42780cttgctcgaa cagctcgtcc gcagccgcaa aaatgcggtc gcgcgtctct ttgttcagtt 42840ccatgttggc tccggtaatt ggtaagaata ataatactct tacctacctt atcagcgcaa 42900gagtttagct gaacagttct cgacttaacg gcaggttttt tagcggctga agggcaggca 42960aaaaaagccc cgcacggtcg gcgggggcaa agggtcagcg ggaaggggat tagcgggcgt 43020cgggcttctt catgcgtcgg ggccgcgctt cttgggatgg agcacgacga agcgcgcacg 43080cgcatcgtcc tcggccctat cggcccgcgt cgcggtcagg aacttgtcgc gcgctaggtc 43140ctccctggtg ggcaccaggg gcatgaactc ggcctgctcg atgtaggtcc actccatgac 43200cgcatcgcag tcgaggccgc gttccttcac cgtctcttgc aggtcgcggt acgcccgctc 43260gttgagcggc tggtaacggg ccaattggtc gtaaatggct gtcggccatg agcggccttt 43320cctgttgagc cagcagccga cgacgaagcc ggcaatgcag gcccctggca caaccaggcc 43380gacgccgggg gcaggggatg gcagcagctc gccaaccagg aaccccgccg cgatgatgcc 43440gatgccggtc aaccagccct tgaaactatc cggccccgaa acacccctgc gcattgcctg 43500gatgctgcgc cggatagctt gcaacatcag gagccgtttc ttttgttcgt cagtcatggt 43560ccgccctcac cagttgttcg tatcggtgtc ggacgaactg aaatcgcaag agctgccggt 43620atcggtccag ccgctgtccg tgtcgctgct gccgaagcac ggcgaggggt ccgcgaacgc 43680cgcagacggc gtatccggcc gcagcgcatc gcccagcatg gccccggtca gcgagccgcc 43740ggccaggtag cccagcatgg tgctgttggt cgccccggcc accagggccg acgtgacgaa 43800atcgccgtca ttccctctgg attgttcgct gctcggcggg gcagtgcgcc gcgccggcgg 43860cgtcgtggat ggctcgggtt ggctggcctg cgacggccgg cgaaaggtgc gcagcagctc 43920gttatcgacc ggctgcggcg tcggggccgc cgccttgcgc tgcggtcggt gttccttctt 43980cggctcgcgc agcttgaaca gcatgatcgc ggaaaccagc agcaacgccg cgcctacgcc 44040tcccgcgatg tagaacagca tcggattcat tcttcggtcc tccttgtagc ggaaccgttg 44100tctgtgcggc gcgggtggcc cgcgccgctg tctttgggga tcagccctcg atgagcgcga 44160ccagtttcac gtcggcaagg ttcgcctcga actcctggcc gtcgtcctcg tacttcaacc 44220aggcatagcc ttccgccggc ggccgacggt tgaggataag gcgggcaggg cgctcgtcgt 44280gctcgacctg gacgatggcc tttttcagct tgtccgggtc cggctccttc gcgccctttt 44340ccttggcgtc cttaccgtcc tggtcgccgt cctcgccgtc ctggccgtcg ccggcctccg 44400cgtcacgctc ggcatcagtc tggccgttga aggcatcgac ggtgttggga tcgcggccct 44460tctcgtccag gaactcgcgc agcagcttga ccgtgccgcg cgtgatttcc tgggtgtcgt 44520cgtcaagcca cgcctcgact tcctccgggc gcttcttgaa ggccgtcacc agctcgttca 44580ccacggtcac gtcgcgcacg cggccggtgt tgaacgcatc ggcgatcttc tccggcaggt 44640ccagcagcgt gacgtgctgg gtgatgaacg ccggcgactt gccgatttcc ttggcgatat 44700cgcctttctt cttgcccttc gccagctcgc ggccaatgaa gtcggcaatt tcgcgcgggg 44760tcagctcgtt gcgttgcagg ttctcgataa cctggtcggc ttcgttgtag tcgttgtcga 44820tgaacgccgg gatggacttc ttgccggccc acttcgagcc acggtagcgg cgggcgccgt 44880gattgatgat atagcggccc ggctgctcct ggttctcgcg caccgaaatg ggtgacttca 44940ccccgcgctc tttgatcgtg gcaccgattt ccgcgatgct ctccggggaa aagccggggt 45000tgtcggccgt ccgcggctga tgcggatctt cgtcgatcag gtccaggtcc agctcgatag 45060ggccggaacc gccctgagac gccgcaggag cgtccaggag gctcgacagg tcgccgatgc 45120tatccaaccc caggccggac ggctgcgccg cgcctgcggc ttcctgagcg gccgcagcgg 45180tgtttttctt ggtggtcttg gcttgagccg cagtcattgg gaaatctcca tcttcgtgaa 45240cacgtaatca gccagggcgc gaacctcttt cgatgccttg cgcgcggccg ttttcttgat 45300cttccagacc ggcacaccgg atgcgagggc atcggcgatg ctgctgcgca ggccaacggt 45360ggccggaatc atcatcttgg ggtacgcggc cagcagctcg gcttggtggc gcgcgtggcg 45420cggattccgc gcatcgacct tgctgggcac catgccaagg aattgcagct tggcgttctt 45480ctggcgcacg ttcgcaatgg tcgtgaccat cttcttgatg ccctggatgc tgtacgcctc 45540aagctcgatg ggggacagca catagtcggc cgcgaagagg gcggccgcca ggccgacgcc 45600aagggtcggg gccgtgtcga tcaggcacac gtcgaagcct tggttcgcca gggccttgat 45660gttcgccccg aacagctcgc gggcgtcgtc cagcgacagc cgttcggcgt tcgccagtac 45720cgggttggac tcgatgaggg cgaggcgcgc ggcctggccg tcgccggctg cgggtgcggt 45780ttcggtccag ccgccggcag ggacagcgcc gaacagcttg cttgcatgca ggccggtagc 45840aaagtccttg agcgtgtagg acgcattgcc ctgggggtcc aggtcgatca cggcaacccg 45900caagccgcgc tcgaaaaagt cgaaggcaag atgcacaagg gtcgaagtct tgccgacgcc 45960gcctttctgg ttggccgtga ccaaagtttt catcgtttgg tttcctgttt tttcttggcg 46020tccgcttccc acttccggac gatgtacgcc tgatgttccg gcagaaccgc cgttacccgc 46080gcgtacccct cgggcaagtt cttgtcctcg aacgcggccc acacgcgatg caccgcttgc 46140gacactgcgc ccctggtcag tcccagcgac gttgcgaacg tcgcctgtgg cttcccatcg 46200actaagacgc cccgcgctat ctcgatggtc tgctgcccca cttccagccc ctggatcgcc 46260tcctggaact ggctttcggt aagccgtttc ttcatggata acacccataa tttgctccgc 46320gccttggttg aacatagcgg tgacagccgc cagcacatga gagaagttta gctaaacatt 46380tctcgcacgt caacaccttt agccgctaaa actcgtcctt ggcgtaacaa aacaaaagcc 46440cggaaaccgg gctttcgtct cttgccgctt atggctctgc acccggctcc atcaccaaca 46500ggtcgcgcac gcgcttcact cggttgcgga tcgacactgc cagcccaaca aagccggttg 46560ccgccgccgc caggatcgcg ccgatgatgc cggccacacc ggccatcgcc caccaggtcg 46620ccgccttccg gttccattcc tgctggtact gcttcgcaat gctggacctc ggctcaccat 46680aggctgaccg ctcgatggcg tatgccgctt ctccccttgg cgtaaaaccc agcgccgcag 46740gcggcattgc catgctgccc gccgctttcc cgaccacgac gcgcgcacca ggcttgcggt 46800ccagaccttc ggccacggcg agctgcgcaa ggacataatc agccgccgac ttggctccac 46860gcgcctcgat cagctcttgc actcgcgcga aatccttggc ctccacggcc gccatgaatc 46920gcgcacgcgg cgaaggctcc gcagggccgg cgtcgtgatc gccgccgaga atgcccttca 46980ccaagttcga cgacacgaaa atcatgctga cggctatcac catcatgcag acggatcgca 47040cgaacccgct gaattgaaca cgagcacggc acccgcgacc actatgccaa gaatgcccaa 47100ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg 47160caggccgcca cccaggccgc cgccctcact gcccggcacc tggtcgctga atgtcgatgc 47220cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt 47280tactgccccg atcccggcaa tggcaaggac tgccagcgct gccatttttg gggtgaggcc 47340gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga 47400gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc 47460cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc 47520ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc 47580cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg 47640atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta 47700tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga 47760tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca 47820acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga 47880gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc gcacacggct 47940tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg 48000caaccagccc ggtgagcgtc ggaaaggcgc tggaagcccc gtagcgacgc ggagaggggc 48060gagacaagcc aagggcgcag gctcgatgcg cagcacgaca tagccggttc tcgcaaggac 48120gagaatttcc ctgcggtgcc cctcaagtgt caatgaaagt ttccaacgcg agccattcgc 48180gagagccttg agtccacgct agatgagagc tttgttgtag gtggaccagt tggtgatttt 48240gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt 48300caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg 48360ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa 48420cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgac 484741347505DNAartificial sequencePHP16972 (ZmCAS1HindIII ProMS45/35SPAT) 13tctagagctc gttcctcgag gaacggtacc tgcggggaag cttacaataa tgtgtgttgt 60taagtcttgt tgcctgtcat cgtctgactg actttcgtca taaatcccgg cctccgtaac 120ccagctttgg gcaagctcac ggatttgatc cggcggaacg ggaatatcga gatgccgggc 180tgaacgctgc agttccagct ttccctttcg ggacaggtac tccagctgat tgattatctg 240ctgaagggtc ttggttccac ctcctggcac aatgcgaatg attacttgag cgcgatcggg 300catccaattt tctcccgtca ggtgcgtggt caagtgctac aaggcacctt tcagtaacga 360gcgaccgtcg atccgtcgcc gggatacgga caaaatggag cgcagtagtc catcgagggc 420ggcgaaagcc tcgccaaaag caatacgttc atctcgcaca gcctccagat ccgatcgagg 480gtcttcggcg taggcagata gaagcatgga tacattgctt gagagtattc cgatggactg 540aagtatggct tccatctttt ctcgtgtgtc tgcatctatt tcgagaaagc ccccgatgcg 600gcgcaccgca acgcgaattg ccatactatc cgaaagtccc agcaggcgcg cttgatagga 660aaaggtttca tactcggccg atcgcagacg ggcactcacg accttgaacc cttcaacttt 720cagggatcga tgctggttga tggtagtctc actcgacgtg gctctggtgt gttttgacat 780agcttcctcc aaagaaagcg gaaggtctgg atactccagc acgaaatgtg cccgggtaga 840cggatggaag tctagccctg ctcaatatga aatcaacagt acatttacag tcaatactga 900atatacttgc tacatttgca attgtcttat aacgaatgtg aaataaaaat agtgtaacaa 960cgcttttact catcgataat cacaaaaaca tttatacgaa caaaaataca aatgcactcc 1020ggtttcacag gataggcggg atcagaatat gcaacttttg acgttttgtt ctttcaaagg 1080gggtgctggc aaaaccaccg cactcatggg cctttgcgct gctttggcaa atgacggtaa 1140acgagtggcc ctctttgatg ccgacgaaaa ccggcctctg acgcgatgga gagaaaacgc 1200cttacaaagc agtactggga tcctcgctgt gaagtctatt ccgccgacga aatgcccctt 1260cttgaagcag cctatgaaaa tgccgagctc gaaggatttg attatgcgtt ggccgatacg 1320cgtggcggct cgagcgagct caacaacaca atcatcgcta gctcaaacct gcttctgatc 1380cccaccatgc taacgccgct cgacatcgat gaggcactat ctacctaccg ctacgtcatc 1440gagctgctgt tgagtgaaaa tttggcaatt cctacagctg ttttgcgcca acgcgtcccg 1500gtcggccgat tgacaacatc gcaacgcagg atgtcagaga cgctagagag ccttccagtt 1560gtaccgtctc ccatgcatga aagagatgca tttgccgcga tgaaagaacg cggcatgttg 1620catcttacat tactaaacac gggaactgat ccgacgatgc gcctcataga gaggaatctt 1680cggattgcga tggaggaagt cgtggtcatt tcgaaactga tcagcaaaat cttggaggct 1740tgaagatggc aattcgcaag cccgcattgt cggtcggcga agcacggcgg cttgctggtg 1800ctcgacccga gatccaccat cccaacccga cacttgttcc ccagaagctg gacctccagc 1860acttgcctga aaaagccgac gagaaagacc agcaacgtga gcctctcgtc gccgatcaca 1920tttacagtcc cgatcgacaa cttaagctaa ctgtggatgc ccttagtcca cctccgtccc 1980cgaaaaagct ccaggttttt ctttcagcgc gaccgcccgc gcctcaagtg tcgaaaacat 2040atgacaacct cgttcggcaa tacagtccct cgaagtcgct acaaatgatt ttaaggcgcg 2100cgttggacga tttcgaaagc atgctggcag atggatcatt tcgcgtggcc ccgaaaagtt 2160atccgatccc ttcaactaca gaaaaatccg ttctcgttca gacctcacgc atgttcccgg 2220ttgcgttgct cgaggtcgct cgaagtcatt ttgatccgtt ggggttggag accgctcgag 2280ctttcggcca caagctggct accgccgcgc tcgcgtcatt ctttgctgga gagaagccat 2340cgagcaattg gtgaagaggg acctatcgga acccctcacc aaatattgag tgtaggtttg 2400aggccgctgg ccgcgtcctc agtcaccttt tgagccagat aattaagagc caaatgcaat 2460tggctcaggc tgccatcgtc cccccgtgcg aaacctgcac gtccgcgtca aagaaataac 2520cggcacctct tgctgttttt atcagttgag ggcttgacgg atccgcctca agtttgcggc 2580gcagccgcaa aatgagaaca tctatactcc tgtcgtaaac ctcctcgtcg cgtactcgac 2640tggcaatgag aagttgctcg cgcgatagaa cgtcgcgggg tttctctaaa aacgcgagga 2700gaagattgaa ctcacctgcc gtaagtttca cctcaccgcc agcttcggac atcaagcgac 2760gttgcctgag attaagtgtc cagtcagtaa aacaaaaaga ccgtcggtct ttggagcgga 2820caacgttggg gcgcacgcgc aaggcaaccc gaatgcgtgc aagaaactct ctcgtactaa 2880acggcttagc gataaaatca cttgctccta gctcgagtgc aacaacttta tccgtctcct 2940caaggcggtc gccactgata attatgattg gaatatcaga ctttgccgcc agatttcgaa 3000cgatctcaag cccatcttca cgacctaaat ttagatcaac aaccacgaca tcgaccgtcg 3060cggaagagag tactctagtg aactgggtgc tgtcggctac cgcggtcact ttgaaggcgt 3120ggatcgtaag gtattcgata ataagatgcc gcatagcgac atcgtcatcg ataagaagaa 3180cgtgtttcaa cggctcacct ttcaatctaa aatctgaacc cttgttcaca gcgcttgaga 3240aattttcacg tgaaggatgt acaatcatct ccagctaaat gggcagttcg tcagaattgc 3300ggctgaccgc ggatgacgaa aatgcgaacc aagtatttca attttatgac aaaagttctc 3360aatcgttgtt acaagtgaaa cgcttcgagg ttacagctac tattgattaa ggagatcgcc 3420tatggtctcg ccccggcgtc gtgcgtccgc cgcgagccag atctcgccta cttcataaac 3480gtcctcatag gcacggaatg gaatgatgac atcgatcgcc gtagagagca tgtcaatcag 3540tgtgcgatct tccaagctag caccttgggc gctacttttg acaagggaaa acagtttctt 3600gaatccttgg attggattcg cgccgtgtat tgttgaaatc gatcccggat gtcccgagac 3660gacttcactc agataagccc atgctgcatc gtcgcgcatc tcgccaagca atatccggtc 3720cggccgcata cgcagacttg cttggagcaa gtgctcggcg ctcacagcac ccagcccagc 3780accgttcttg gagtagagta gtctaacatg attatcgtgt ggaatgacga gttcgagcgt 3840atcttctatg gtgattagcc tttcctgggg ggggatggcg ctgatcaagg tcttgctcat 3900tgttgtcttg ccgcttccgg tagggccaca tagcaacatc gtcagtcggc tgacgacgca 3960tgcgtgcaga aacgcttcca aatccccgtt gtcaaaatgc tgaaggatag cttcatcatc 4020ctgattttgg cgtttccttc gtgtctgcca ctggttccac ctcgaagcat cataacggga 4080ggagacttct ttaagaccag aaacacgcga gcttggccgt cgaatggtca agctgacggt 4140gcccgaggga acggtcggcg gcagacagat ttgtagtcgt tcaccaccag gaagttcagt 4200ggcgcagagg gggttacgtg gtccgacatc ctgctttctc agcgcgcccg ctaaaatagc 4260gatatcttca agatcatcat aagagacggg caaaggcatc ttggtaaaaa tgccggcttg 4320gcgcacaaat gcctctccag gtcgattgat cgcaatttct tcagtcttcg ggtcatcgag 4380ccattccaaa atcggcttca gaagaaagcg tagttgcgga tccacttcca tttacaatgt 4440atcctatctc taagcggaaa tttgaattca ttaagagcgg cggttcctcc cccgcgtggc 4500gccgccagtc aggcggagct ggtaaacacc aaagaaatcg aggtcccgtg ctacgaaaat 4560ggaaacggtg tcaccctgat tcttcttcag ggttggcggt atgttgatgg ttgccttaag 4620ggctgtctca gttgtctgct caccgttatt ttgaaagctg ttgaagctca tcccgccacc 4680cgagctgccg gcgtaggtgc tagctgcctg gaaggcgcct tgaacaacac tcaagagcat 4740agctccgcta aaacgctgcc agaagtggct gtcgaccgag cccggcaatc ctgagcgacc 4800gagttcgtcc gcgcttggcg atgttaacga gatcatcgca tggtcaggtg tctcggcgcg 4860atcccacaac acaaaaacgc gcccatctcc ctgttgcaag ccacgctgta tttcgccaac 4920aacggtggtg ccacgatcaa gaagcacgat attgttcgtt gttccacgaa tatcctgagg 4980caagacacac tttacatagc ctgccaaatt tgtgtcgatt gcggtttgca agatgcacgg 5040aattattgtc ccttgcgtta ccataaaatc ggggtgcggc aagagcgtgg cgctgctggg 5100ctgcagctcg gtgggtttca tacgtatcga caaatcgttc tcgccggaca cttcgccatt 5160cggcaaggag ttgtcgtcac gcttgccttc ttgtcttcgg cccgtgtcgc cctgaatggc 5220gcgtttgctg accccttgat cgccgctgct atatgcaaaa atcggtgttt cttccggccg 5280tggctcatgc cgctccggtt cgcccctcgg cggtagagga gcagcaggct gaacagcctc 5340ttgaaccgct ggaggatccg gcggcacctc aatcggagct ggatgaaatg gcttggtgtt 5400tgttgcgatc aaagttgacg gcgatgcgtt ctcattcacc ttcttttggc gcccacctag 5460ccaaatgagg cttaatgata acgcgagaac gacacctccg acgatcaatt tctgagaccc 5520cgaaagacgc cggcgatgtt tgtcggagac cagggatcca gatgcatcaa cctcatgtgc 5580cgcttgctga ctatcgttat tcatcccttc gcccccttca ggacgcgttt cacatcgggc 5640ctcaccgtgc ccgtttgcgg cctttggcca acgggatcgt aagcggtgtt ccagatacat 5700agtactgtgt ggccatccct cagacgccaa cctcgggaaa ccgaagaaat ctcgacatcg 5760ctccctttaa ctgaatagtt ggcaacagct tccttgccat caggattgat ggtgtagatg 5820gagggtatgc gtacattgcc cggaaagtgg aataccgtcg taaatccatt gtcgaagact 5880tcgagtggca acagcgaacg atcgccttgg gcgacgtagt gccaattact gtccgccgca 5940ccaagggctg tgacaggctg atccaataaa ttctcagctt tccgttgata ttgtgcttcc 6000gcgtgtagtc tgtccacaac agccttctgt tgtgcctccc ttcgccgagc cgccgcatcg 6060tcggcggggt aggcgaattg gacgctgtaa tagagatcgg gctgctcttt atcgaggtgg 6120gacagagtct tggaacttat actgaaaaca taacggcgca tcccggagtc gcttgcggtt 6180agcacgatta ctggctgagg cgtgaggacc tggcttgcct tgaaaaatag ataatttccc 6240cgcggtaggg ctgctagatc tttgctattt gaaacggcaa ccgctgtcac cgtttcgttc 6300gtggcgaatg ttacgaccaa agtagctcca accgccgtcg agaggcgcac cacttgatcg 6360ggattgtaag ccaaataacg catgcgcgga tctagcttgc ccgccattgg agtgtcttca 6420gcctccgcac cagtcgcagc ggcaaataaa catgctaaaa tgaaaagtgc ttttctgatc 6480atggttcgct gtggcctacg tttgaaacgg tatcttccga tgtctgatag gaggtgacaa 6540ccagacctgc cgggttggtt agtctcaatc tgccgggcaa gctggtcacc ttttcgtagc 6600gaactgtcgc ggtccacgta ctcaccacag gcattttgcc gtcaacgacg agggtccttt 6660tatagcgaat ttgctgcgtg cttggagtta catcatttga agcgatgtgc tcgacctcca 6720ccctgccgcg tttgccaaga atgacttgag gcgaactggg attgggatag ttgaagaatt 6780gctggtaatc ctggcgcact gttggggcac tgaagttcga taccaggtcg taggcgtact 6840gagcggtgtc ggcatcataa ctctcgcgca ggcgaacgta ctcccacaat gaggcgttaa 6900cgacggcctc ctcttgagtt gcaggcaatc gcgagacaga cacctcgctg tcaacggtgc 6960cgtccggccg tatccataga tatacgggca caagcctgct caacggcacc attgtggcta 7020tagcgaacgc ttgagcaaca tttcccaaaa tcgcgatagc tgcgacagct gcaatgagtt 7080tggagagacg tcgcgccgat ttcgctcgcg cggtttgaaa ggcttctact tccttatagt 7140gctcggcaag gctttcgcgc gccactagca tggcatattc aggccccgtc atagcgtcca 7200cccgaattgc cgagctgaag atctgacgga gtaggctgcc atcgccccac attcagcggg 7260aagatcgggc ctttgcagct cgctaatgtg tcgtttgtct ggcagccgct caaagcgaca 7320actaggcaca gcaggcaata cttcatagaa ttctccattg aggcgaattt ttgcgcgacc 7380tagcctcgct caacctgagc gaagcgacgg tacaagctgc tggcagattg ggttgcgccg 7440ctccagtaac tgcctccaat gttgccggcg atcgccggca aagcgacaat gagcgcatcc 7500cctgtcagaa aaaacatatc gagttcgtaa agaccaatga tcttggccgc ggtcgtaccg 7560gcgaaggtga ttacaccaag cataagggtg agcgcagtcg cttcggttag gatgacgatc 7620gttgccacga ggtttaagag gagaagcaag agaccgtagg tgataagttg cccgatccac 7680ttagctgcga tgtcccgcgt gcgatcaaaa atatatccga cgaggatcag aggcccgatc 7740gcgagaagca ctttcgtgag aattccaacg gcgtcgtaaa ctccgaaggc agaccagagc 7800gtgccgtaaa ggacccactg tgccccttgg aaagcaagga tgtcctggtc gttcatcgga 7860ccgatttcgg atgcgatttt ctgaaaaacg gcctgggtca cggcgaacat tgtatccaac 7920tgtgccggaa cagtctgcag aggcaagccg gttacactaa actgctgaac aaagtttggg 7980accgtctttt cgaagatgga aaccacatag tcttggtagt tagcctgccc aacaattaga 8040gcaacaacga tggtgaccgt gatcacccga gtgataccgc tacgggtatc gacttcgccg 8100cgtatgacta aaataccctg aacaataatc caaagagtga cacaggcgat caatggcgca 8160ctcaccgcct cctggatagt ctcaagcatc gagtccaagc ctgtcgtgaa ggctacatcg 8220aagatcgtat gaatggccgt aaacggcgcc ggaatcgtga aattcatcga ttggacctga 8280acttgactgg tttgtcgcat aatgttggat aaaatgagct cgcattcggc gaggatgcgg 8340gcggatgaac aaatcgccca gccttagggg agggcaccaa agatgacagc ggtcttttga 8400tgctccttgc gttgagcggc cgcctcttcc

gcctcgtgaa ggccggcctg cgcggtagtc 8460atcgttaata ggcttgtcgc ctgtacattt tgaatcattg cgtcatggat ctgcttgaga 8520agcaaaccat tggtcacggt tgcctgcatg atattgcgag atcgggaaag ctgagcagac 8580gtatcagcat tcgccgtcaa gcgtttgtcc atcgtttcca gattgtcagc cgcaatgcca 8640gcgctgtttg cggaaccggt gatctgcgat cgcaacaggt ccgcttcagc atcactaccc 8700acgactgcac gatctgtatc gctggtgatc gcacgtgccg tggtcgacat tggcattcgc 8760ggcgaaaaca tttcattgtc taggtccttc gtcgaaggat actgattttt ctggttgagc 8820gaagtcagta gtccagtaac gccgtaggcc gacgtcaaca tcgtaaccat cgctatagtc 8880tgagtgagat tctccgcagt cgcgagcgca gtcgcgagcg tctcagcctc cgttgccggg 8940tcgctaacaa caaactgcgc ccgcgcgggc tgaatatata gaaagctgca ggtcaaaact 9000gttgcaataa gttgcgtcgt cttcatcgtt tcctacctta tcaatcttct gcctcgtggt 9060gacgggccat gaattcgctg agccagccag atgagttgcc ttcttgtgcc tcgcgtagtc 9120gagttgcaaa gcgcaccgtg ttggcacgcc ccgaaagcac ggcgacatat tcacgcatat 9180cccgcagatc aaattcgcag atgacgcttc cactttctcg tttaagaaga aacttacggc 9240tgccgaccgt catgtcttca cggatcgcct gaaattcctt ttcggtacat ttcagtccat 9300cgacataagc cgatcgatct gcggttggtg atggatagaa aatcttcgtc atacattgcg 9360caaccaagct ggctcctagc ggcgattcca gaacatgctc tggttgctgc gttgccagta 9420ttagcatccc gttgtttttt cgaacggtca ggaggaattt gtcgacgaca gtcgaaaatt 9480tagggtttaa caaataggcg cgaaactcat cgcagctcat cacaaaacgg cggccgtcga 9540tcatggctcc aatccgatgc aggagatatg ctgcagcggg agcgcatact tcctcgtatt 9600cgagaagatg cgtcatgtcg aagccggtaa tcgacggatc taactttact tcgtcaactt 9660cgccgtcaaa tgcccagcca agcgcatggc cccggcacca gcgttggagc cgcgctcctg 9720cgccttcggc gggcccatgc aacaaaaatt cacgtaaccc cgcgattgaa cgcatttgtg 9780gatcaaacga gagctgacga tggataccac ggaccagacg gcggttctct tccggagaaa 9840tcccaccccg accatcactc tcgatgagag ccacgatcca ttcgcgcaga aaatcgtgtg 9900aggctgctgt gttttctagg ccacgcaacg gcgccaaccc gctgggtgtg cctctgtgaa 9960gtgccaaata tgttcctcct gtggcgcgaa ccagcaattc gccaccccgg tccttgtcaa 10020agaacacgac cgtacctgca cggtcgacca tgctctgttc gagcatggct agaacaaaca 10080tcatgagcgt cgtcttaccc ctcccgatag gcccgaatat tgccgtcatg ccaacatcgt 10140gctcatgcgg gatatagtcg aaaggcgttc cgccattggt acgaaatcgg gcaatcgcgt 10200tgccccagtg gcctgagctg gcgccctctg gaaagttttc gaaagagaca aaccctgcga 10260aattgcgtga agtgattgcg ccagggcgtg tgcgccactt aaaattcccc ggcaattggg 10320accaataggc cgcttccata ccaatacctt cttggacaac cacggcacct gcatccgcca 10380ttcgtgtccg agcccgcgcg cccctgtccc caagactatt gagatcgtct gcatagacgc 10440aaaggctcaa atgatgtgag cccataacga attcgttgct cgcaagtgcg tcctcagcct 10500cggataattt gccgatttga gtcacggctt tatcgccgga actcagcatc tggctcgatt 10560tgaggctaag tttcgcgtgc gcttgcgggc gagtcaggaa cgaaaaactc tgcgtgagaa 10620caagtggaaa atcgagggat agcagcgcgt tgagcatgcc cggccgtgtt tttgcagggt 10680attcgcgaaa cgaatagatg gatccaacgt aactgtcttt tggcgttctg atctcgagtc 10740ctcgcttgcc gcaaatgact ctgtcggtat aaatcgaagc gccgagtgag ccgctgacga 10800ccggaaccgg tgtgaaccga ccagtcatga tcaaccgtag cgcttcgcca atttcggtga 10860agagcacacc ctgcttctcg cggatgccaa gacgatgcag gccatacgct ttaagagagc 10920cagcgacaac atgccaaaga tcttccatgt tcctgatctg gcccgtgaga tcgttttccc 10980tttttccgct tagcttggtg aacctcctct ttaccttccc taaagccgcc tgtgggtaga 11040caatcaacgt aaggaagtgt tcattgcgga ggagttggcc ggagagcacg cgctgttcaa 11100aagcttcgtt caggctagcg gcgaaaacac tacggaagtg tcgcggcgcc gatgatggca 11160cgtcggcatg acgtacgagg tgagcatata ttgacacatg atcatcagcg atattgcgca 11220acagcgtgtt gaacgcacga caacgcgcat tgcgcatttc agtttcctca agctcgaatg 11280caacgccatc aattctcgca atggtcatga tcgatccgtc ttcaagaagg acgatatggt 11340cgctgaggtg gccaatataa gggagataga tctcaccgga tctttcggtc gttccactcg 11400cgccgagcat cacaccattc ctctccctcg tgggggaacc ctaattggat ttgggctaac 11460agtagcgccc ccccaaactg cactatcaat gcttcttccc gcggtccgca aaaatagcag 11520gacgacgctc gccgcattgt agtctcgctc cacgatgagc cgggctgcaa accataacgg 11580cacgagaacg acttcgtaga gcgggttctg aacgataacg atgacaaagc cggcgaacat 11640catgaataac cctgccaatg tcagtggcac cccaagaaac aatgcgggcc gtgtggctgc 11700gaggtaaagg gtcgattctt ccaaacgatc agccatcaac taccgccagt gagcgtttgg 11760ccgaggaagc tcgccccaaa catgataaca atgccgccga cgacgccggc aaccagccca 11820agcgaagccc gcccgaacat ccaggagatc ccgatagcga caatgccgag aacagcgagt 11880gactggccga acggaccaag gataaacgtg catatattgt taaccattgt ggcggggtca 11940gtgccgccac ccgcagattg cgctgcggcg ggtccggatg aggaaatgct ccatgcaatt 12000gcaccgcaca agcttggggc gcagctcgat atcacgcgca tcatcgcatt cgagagcgag 12060aggcgattta gatgtaaacg gtatctctca aagcatcgca tcaatgcgca cctccttagt 12120ataagtcgaa taagacttga ttgtcgtctg cggatttgcc gttgtcctgg tgtggcggtg 12180gcggagcgat taaaccgcca gcgccatcct cctgcgagcg gcgctgatat gacccccaaa 12240catcccacgt ctcttcggat tttagcgcct cgtgatcgtc ttttggaggc tcgattaacg 12300cgggcaccag cgattgagca gctgtttcaa cttttcgcac gtagccgttt gcaaaaccgc 12360cgatgaaatt accggtgttg taagcggaga tcgcccgacg aagcgcaaat tgcttctcgt 12420caatcgtttc gccgcctgca taacgacttt tcagcatgtt tgcagcggca gataatgatg 12480tgcacgcctg gagcgcaccg tcaggtgtca gaccgagcat agaaaaattt cgagagttta 12540tttgcatgag gccaacatcc agcgaatgcc gtgcatcgag acggtgcctg acgacttggg 12600ttgcttggct gtgatcttgc cagtgaagcg tttcgccggt cgtgttgtca tgaatcgcta 12660aaggatcaaa gcgactctcc accttagcta tcgccgcaag cgtagatgtc gcaactgatg 12720gggcacactt gcgagcaaca tggtcaaact cagcagatga gagtggcgtg gcaaggctcg 12780acgaacagaa ggagaccatc aaggcaagag aaagcgaccc cgatctctta agcatacctt 12840atctccttag ctcgcaacta acaccgcctc tcccgttgga agaagtgcgt tgttttatgt 12900tgaagattat cgggagggtc ggttactcga aaattttcaa ttgcttcttt atgatttcaa 12960ttgaagcgag aaacctcgcc cggcgtcttg gaacgcaaca tggaccgaga accgcgcatc 13020catgactaag caaccggatc gacctattca ggccgcagtt ggtcaggtca ggctcagaac 13080gaaaatgctc ggcgaggtta cgctgtctgt aaacccattc gatgaacggg aagcttcctt 13140ccgattgctc ttggcaggaa tattggccca tgcctgcttg cgctttgcaa atgctcttat 13200cgcgttggta tcatatgcct tgtccgccag cagaaacgca ctctaagcga ttatttgtaa 13260aaatgtttcg gtcatgcggc ggtcatgggc ttgacccgct gtcagcgcaa gacggatcgg 13320tcaaccgtcg gcatcgacaa cagcgtgaat cttggtggtc aaaccgccac gggaacgtcc 13380catacagcca tcgtcttgat cccgctgttt cccgtcgccg catgttggtg gacgcggaca 13440caggaactgt caatcatgac gacattctat cgaaagcctt ggaaatcaca ctcagaatat 13500gatcccagac gtctgcctca cgccatcgta caaagcgatt gtagcaggtt gtacaggaac 13560cgtatcgatc aggaacgtct gcccagggcg ggcccgtccg gaagcgccac aagatgacat 13620tgatcacccg cgtcaacgcg cggcacgcga cgcggcttat ttgggaacaa aggactgaac 13680aacagtccat tcgaaatcgg tgacatcaaa gcggggacgg gttatcagtg gcctccaagt 13740caagcctcaa tgaatcaaaa tcagaccgat ttgcaaacct gatttatgag tgtgcggcct 13800aaatgatgaa atcgtccttc tagatcgcct ccgtggtgta gcaacacctc gcagtatcgc 13860cgtgctgacc ttggccaggg aattgactgg caagggtgct ttcacatgac cgctcttttg 13920gccgcgatag atgatttcgt tgctgctttg ggcacgtaga aggagagaag tcatatcgga 13980gaaattcctc ctggcgcgag agcctgctct atcgcgacgg catcccactg tcgggaacag 14040accggatcat tcacgaggcg aaagtcgtca acacatgcgt tataggcatc ttcccttgaa 14100ggatgatctt gttgctgcca atctggaggt gcggcagccg caggcagatg cgatctcagc 14160gcaacttgcg gcaaaacatc tcactcacct gaaaaccact agcgagtctc gcgatcagac 14220gaaggccttt tacttaacga cacaatatcc gatgtctgca tcacaggcgt cgctatccca 14280gtcaatacta aagcggtgca ggaactaaag attactgatg acttaggcgt gccacgaggc 14340ctgagacgac gcgcgtagac agttttttga aatcattatc aaagtgatgg cctccgctga 14400agcctatcac ctctgcgccg gtctgtcgga gagatgggca agcattatta cggtcttcgc 14460gcccgtacat gcattggacg attgcagggt caatggatct gagatcatcc agaggattgc 14520cgcccttacc ttccgtttcg agttggagcc agcccctaaa tgagacgaca tagtcgactt 14580gatgtgacaa tgccaagaga gagatttgct taacccgatt tttttgctca agcgtaagcc 14640tattgaagct tgccggcatg acgtccgcgc cgaaagaata tcctacaagt aaaacattct 14700gcacaccgaa atgcttggtg tagacatcga ttatgtgacc aagatcctta gcagtttcgc 14760ttggggaccg ctccgaccag aaataccgaa gtgaactgac gccaatgaca ggaatccctt 14820ccgtctgcag ataggtacca tcgatagatc tgctgcctcg cgcgtttcgg tgatgacggt 14880gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 14940gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 15000atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 15060agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 15120aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 15180ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 15240gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 15300aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 15360gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 15420ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 15480cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 15540cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 15600gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 15660cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 15720agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 15780ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 15840ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 15900gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 15960cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 16020attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 16080accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 16140ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 16200gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 16260agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 16320ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 16380ttgttgccat tgctgcaggg gggggggggg ggggggactt ccattgttca ttccacggac 16440aaaaacagag aaaggaaacg acagaggcca aaaagcctcg ctttcagcac ctgtcgtttc 16500ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa 16560cgccttaaac cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac 16620ctgtcggatc accggaaagg acccgtaaag tgataatgat tatcatctac atatcacaac 16680gtgcgtggag gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat 16740tgatctgcat caacttaacg taaaaacaac ttcagacaat acaaatcagc gacactgaat 16800acggggcaac ctcatgtccc cccccccccc ccccctgcag ggcatcgtgg tgtcacgctc 16860gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 16920ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 16980gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 17040gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 17100gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 17160tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 17220gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 17280agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 17340aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 17400ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 17460gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 17520agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 17580tcttcaagaa ttggtcgacg atcttgctgc gttcggatat tttcgtggag ttcccgccac 17640agacccggat tgaaggcgag atccagcaac tcgcgccaga tcatcctgtg acggaacttt 17700ggcgcgtgat gactggccag gacgtcggcc gaaagagcga caagcagatc acgcttttcg 17760acagcgtcgg atttgcgatc gaggattttt cggcgctgcg ctacgtccgc gaccgcgttg 17820agggatcaag ccacagcagc ccactcgacc ttctagccga cccagacgag ccaagggatc 17880tttttggaat gctgctccgt cgtcaggctt tccgacgttt gggtggttga acagaagtca 17940ttatcgtacg gaatgccaag cactcccgag gggaaccctg tggttggcat gcacatacaa 18000atggacgaac ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct 18060cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag 18120gcgggaaacg acaatctgat catgagcgga gaattaaggg agtcacgtta tgacccccgc 18180cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag 18240ccactcagcc caagcttttt ggaaggctaa ggagaggaag ccggcgagaa ggagggggcg 18300ttttacgtgt cactgtcctg tcgtgttggc tgttgacacg aatcatttct tccgcgcgtg 18360ggaagaagaa gatgcacatt agcggcctga agtagagatg tcaatgggga attccccagc 18420ggggattaac tccccagacc cgtacccatg aacatagacc ggcccccatc cccgaacccg 18480aacccgacct cgggtacgaa aatcctccca tacccattcc cgaccgggta ctaaataccc 18540atgggtatcc atacccgacc cgattattca aaaattaatg ggctttttat ttgttaaccg 18600gcggacgcaa tgcttgggac tctaggtttt tttactttgt tgaccggctg gcggctgggc 18660tttttcctac aggcccaaag ttggtcggca gccactaggc cacacgtcac aggcagccca 18720caagtaaatg tcgttggatt gctggatggt ggaataaaaa tcctagatgc tagattgttc 18780tggttccggg tatttttctc catggctaat cgggtttggg tttagccctc ccaaacccga 18840acccgccata cccgatgggt aagggattta ttccaaatct atacccatgg ggatttgttt 18900taacccatac cttaacccta atagaggaat tccccacggg taatcgggtt tcggggccca 18960ttgacatctc tagactgaag gcgtccaact caaatcatta aaaagtgttg acgcacgcgc 19020tgatgcgccg gccgcacagc acaggctgca cagcccgttt aatcagcgat ggagccccgg 19080ccgtcagcca gccaggtccg gcgtccgggt ctgcgccctg cggcgtcact gctgtcgcca 19140ccgtctccga tggtcccaca tccatccagc gggccgcgcg tggtacaaaa ggctcttcct 19200cgccgtcagg tgcagctgcc caaacaccag acacagactc caccaccccg cttcgatctt 19260ctgttgcagc tgaaatctgt cagattctgc agttcattcc tcatggagaa gaggaacctg 19320cagtggcggc gagggcgtga tggcatcgtg cagtaccctc acctcttctt cgcggccctg 19380gcgctggccc tcctagtcgc ggaccgttcg gcctcagtcc gctggccgag gtcgactacc 19440ggccggtgaa gcacgagctc gcgccgtacg gggaggtcat gggcagctgg cccagagaca 19500atgccagccg gctcaggcgc gggaggctgg agttcgtcgg cgaggtgttc gggccggagt 19560ctatcgagtt cgatctccag ggccgcgggc cgtacgccgg cctcgccgac ggccgcgtcg 19620tgcggtggat gggcgaggag gccgggtggg agacgttcgc cggtcatgaa tcctgactgg 19680taagtgctcg atatgcctcc ggcgtccact cgttacagtg ctataatata gtagtactaa 19740gatattttga tctgattttt tgcattcttg ggagaaacgt catgcaaaat ttgttgtttc 19800ttggcaaagg tcagaagaag tctgtgccaa tggagtgaac tcaacgacga ggaagcagca 19860cgagaaggag gagttctgcg gcggccgctc ggcctgaggt tccacgggga gaccggcgag 19920ctctacgtcg ccgacgcgta ctacggtctc atggtcgttg gccagagcgg cggcgtggcg 19980tcctccgtcg cgagggaagc cgacggggac cccatccggt tcgcgaacga cctcgatgtg 20040cacaggaatg gatccgtatt cttcactgac acgagcatga gatacagcag aaagtgagca 20100aagcagcgta acaatccggc ttctcatttt caaacgcctc tgtattctct gctgaaagag 20160tagctcacca gacaagagct gaatttgcag ggaccatctg aacatcctgt tagaaggaga 20220aggcaccggg aggctgctca ggtatgatcc agaaacaagc ggtgtccatg tcgtgctcaa 20280ggggctggtg ttcccaaacg gcgtgcagat ctcagaggac catcagtttc ttctcttctc 20340cgagacaaca aactgcaggt aacaaaaata ctatctgacg atgctcatga ttctaccgta 20400tccatagtca tgaacacaaa ccacacgaat ctggccttga ccaggataat gaggtactgg 20460ctggaaggcc caagagcggg cgaggtagag gtgttcgcga acctgccggg cttccccgac 20520aacgtgcgct ccaacggcag gggccagttc tgggtggcga tcgactgctg ccggaccgag 20580gaggtgttcc caagagcgtg gctccggacc ctgtacttca agttcccgct gtcgctcaag 20640gtgctcactt ggaaggccgc caggaggatg cacacggtgc tcgcgctcct cgacggcgaa 20700gggcgcgtcg tggaggtgct cgaggaccgg ggccacgagg tgatgaagct ggtgagcgag 20760gtgcgggagg tgggccgcaa gctgtggatc ggaaccgtgg cgcacaacca catcgccacc 20820atcccctacc ctttagagga ctaaccatga tctatgctgt ttcaatgcct cctaatctgt 20880gtacgtctat aaatgtctaa tgcagctcat ggttgtaatc ttgtttgtgt ttggcaaatt 20940ggcataataa tggacagatt caatgggcat tggtgctgta gtcgcatcac actaattgaa 21000tgggatcatg ttgagctctc actttgctac aatttgctcc agcttgtacg gttgtaccct 21060cttgctcgtc tatagtaagg gccatctaaa aaaaactcaa attagatctg caatacaagt 21120atgattgggc cgaatttgga ttgtcacggg tccgcgaccg cgaattgggc tccggtttga 21180tttagccgac atagtagtga ccgacccgag ccggccggcg agaccaaacc gagcggacgc 21240ccgccatgca tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact 21300ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac 21360atggtggagc acgacacgct tgtctactcc aaaaatatca aagatacagt ctcagaagac 21420caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat 21480tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa 21540tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc 21600aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 21660tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 21720tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacaggg 21780tacccgggga tccaccatgt ctccggagag gagaccagtt gagattaggc cagctacagc 21840agctgatatg gccgcggttt gtgatatcgt taaccattac attgagacgt ctacagtgaa 21900ctttaggaca gagccacaaa caccacaaga gtggattgat gatctagaga ggttgcaaga 21960tagataccct tggttggttg ctgaggttga gggtgttgtg gctggtattg cttacgctgg 22020gccctggaag gctaggaacg cttacgattg gacagttgag agtactgttt acgtgtcaca 22080taggcatcaa aggttgggcc taggatccac attgtacaca catttgctta agtctatgga 22140ggcgcaaggt tttaagtctg tggttgctgt tataggcctt ccaaacgatc catctgttag 22200gttgcatgag gctttgggat acacagcccg gggtacattg cgcgcagctg gatacaagca 22260tggtggatgg catgatgttg gtttttggca aagggatttt gagttgccag ctcctccaag 22320gccagttagg ccagttaccc agatctgagt cgacctgcag gcatgccgct gaaatcacca 22380gtctctctct acaaatctat ctctctctat aataatgtgt gagtagttcc cagataaggg 22440aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt 22500atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag 22560tgggtaccga gctcgaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct 22620aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga 22680ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt 22740cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag 22800aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga 22860ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat catctccctc gcagagatcc 22920gaattatcag ccttcttatt catttctcgc ttaaccgtga caggctgtcg atcttgagaa 22980ctatgccgac ataataggaa atcgctggat aaagccgctg aggaagctga gtggcgctat 23040ttctttagaa gtgaacgttg acgatcgtcg accgtacccc gatgaattaa ttcggacgta 23100cgttctgaac acagctggat acttacttgg gcgattgtca tacatgacat caacaatgta 23160cccgtttgtg taaccgtctc ttggaggttc gtatgacact agtggttccc ctcagcttgc 23220gactagatgt tgaggcctaa cattttatta gagagcaggc tagttgctta gatacatgat 23280cttcaggccg ttatctgtca gggcaagcga aaattggcca tttatgacga ccaatgcccc 23340gcagaagctc ccatctttgc cgccatagac gccgcgcccc ccttttgggg tgtagaacat 23400ccttttgcca gatgtggaaa agaagttcgt tgtcccattg ttggcaatga cgtagtagcc 23460ggcgaaagtg cgagacccat ttgcgctata

tataagccta cgatttccgt tgcgactatt 23520gtcgtaattg gatgaactat tatcgtagtt gctctcagag ttgtcgtaat ttgatggact 23580attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga gaaatgtcgt agttggatgg 23640ggagtagtca tagggaagac gagcttcatc cactaaaaca attggcaggt cagcaagtgc 23700ctgccccgat gccatcgcaa gtacgaggct tagaaccacc ttcaacagat cgcgcatagt 23760cttccccagc tctctaacgc ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg 23820aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtgaacaaa 23880ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat aagcctgcct 23940agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc 24000gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag 24060cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc 24120atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg 24180acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc 24240gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg 24300cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac 24360ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt 24420gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat 24480atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa 24540cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc 24600ggcgatcacc gcttccctca tgatgtttaa ctcctgaatt aagccgcgcc gcgaagcggt 24660gtcggcttga atgaattgtt aggcgtcatc ctgtgctccc gagaaccagt accagtacat 24720cgctgtttcg ttcgagactt gaggtctagt tttatacgtg aacaggtcaa tgccgccgag 24780agtaaagcca cattttgcgt acaaattgca ggcaggtaca ttgttcgttt gtgtctctaa 24840tcgtatgcca aggagctgtc tgcttagtgc ccactttttc gcaaattcga tgagactgtg 24900cgcgactcct ttgcctcggt gcgtgtgcga cacaacaatg tgttcgatag aggctagatc 24960gttccatgtt gagttgagtt caatcttccc gacaagctct tggtcgatga atgcgccata 25020gcaagcagag tcttcatcag agtcatcatc cgagatgtaa tccttccggt aggggctcac 25080acttctggta gatagttcaa agccttggtc ggataggtgc acatcgaaca cttcacgaac 25140aatgaaatgg ttctcagcat ccaatgtttc cgccacctgc tcagggatca ccgaaatctt 25200catatgacgc ctaacgcctg gcacagcgga tcgcaaacct ggcgcggctt ttggcacaaa 25260aggcgtgaca ggtttgcgaa tccgttgctg ccacttgtta acccttttgc cagatttggt 25320aactataatt tatgttagag gcgaagtctt gggtaaaaac tggcctaaaa ttgctgggga 25380tttcaggaaa gtaaacatca ccttccggct cgatgtctat tgtagatata tgtagtgtat 25440ctacttgatc gggggatctg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 25500cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 25560gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 25620cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga 25680gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 25740ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 25800cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 25860gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 25920tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 25980agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 26040tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 26100cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 26160ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 26220ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 26280ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 26340ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 26400cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 26460gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 26520atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 26580ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 26640gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 26700tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 26760ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 26820taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 26880gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 26940gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 27000ctgcaggggg gggggggggg ggggacttcc attgttcatt ccacggacaa aaacagagaa 27060aggaaacgac agaggccaaa aagcctcgct ttcagcacct gtcgtttcct ttcttttcag 27120agggtatttt aaataaaaac attaagttat gacgaagaag aacggaaacg ccttaaaccg 27180gaaaattttc ataaatagcg aaaacccgcg aggtcgccgc cccgtaacct gtcggatcac 27240cggaaaggac ccgtaaagtg ataatgatta tcatctacat atcacaacgt gcgtggaggc 27300catcaaacca cgtcaaataa tcaattatga cgcaggtatc gtattaattg atctgcatca 27360acttaacgta aaaacaactt cagacaatac aaatcagcga cactgaatac ggggcaacct 27420catgtccccc cccccccccc ccctgcaggc atcgtggtgt cacgctcgtc gtttggtatg 27480gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 27540aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 27600ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 27660tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 27720ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta 27780aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 27840ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 27900ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 27960agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 28020tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 28080ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 28140atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattc 28200ggagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa 28260ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc 28320agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt 28380acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt 28440tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca 28500gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac ttttgctgag 28560ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag 28620ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact 28680ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga 28740ggcagacctc agcgccagaa ggccgccaga gaggccgagc gcggccgtga ggcttggacg 28800ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa 28860gggggagggg atgttgtcta catggctctg ctgtagtgag tgggttgcgc tccggcagcg 28920gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt cctgacaacg agcctccttt 28980tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt 29040cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc 29100cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga 29160agtagctgat tgtcatcagc gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga 29220ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat 29280ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct gaagctgcgg gcattcccga 29340tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga atgcgtatga ttctccgcca 29400gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg 29460ccggctgctg aacccccaac cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga 29520cctcgttcaa caggtccagg gcggcacgga tcactgtatt cggctgcaac tttgtcatgc 29580ttgacacttt atcactgata aacataatat gtccaccaac ttatcagtga taaagaatcc 29640gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca gacgtgaaac ccaacatacc 29700cctgatcgta attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc 29760ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg cccactatgg 29820cattctgctg gcgctgtatg cgttggtgca atttgcctgc gcacctgtgc tgggcgcgct 29880gtcggatcgt ttcgggcggc ggccaatctt gctcgtctcg ctggccggcg ccactgtcga 29940ctacgccatc atggcgacag cgcctttcct ttgggttctc tatatcgggc ggatcgtggc 30000cggcatcacc ggggcgactg gggcggtagc cggcgcttat attgccgata tcactgatgg 30060cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt ttcgggttcg ggatggtcgc 30120gggacctgtg ctcggtgggc tgatgggcgg tttctccccc cacgctccgt tcttcgccgc 30180ggcagccttg aacggcctca atttcctgac gggctgtttc cttttgccgg agtcgcacaa 30240aggcgaacgc cggccgttac gccgggaggc tctcaacccg ctcgcttcgt tccggtgggc 30300ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc ttcatcatgc aacttgtcgg 30360acaggtgccg gccgcgcttt gggtcatttt cggcgaggat cgctttcact gggacgcgac 30420cacgatcggc atttcgcttg ccgcatttgg cattctgcat tcactcgccc aggcaatgat 30480caccggccct gtagccgccc ggctcggcga aaggcgggca ctcatgctcg gaatgattgc 30540cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg ggatggatgg cgttcccgat 30600catggtcctg cttgcttcgg gtggcatcgg aatgccggcg ctgcaagcaa tgttgtccag 30660gcaggtggat gaggaacgtc aggggcagct gcaaggctca ctggcggcgc tcaccagcct 30720gacctcgatc gtcggacccc tcctcttcac ggcgatctat gcggcttcta taacaacgtg 30780gaacgggtgg gcatggattg caggcgctgc cctctacttg ctctgcctgc cggcgctgcg 30840tcgcgggctt tggagcggcg cagggcaacg agccgatcgc tgatcgtgga aacgataggc 30900ctatgccatg cgggtcaagg cgacttccgg caagctatac gcgccctagg agtgcggttg 30960gaacgttggc ccagccagat actcccgatc acgagcagga cgccgatgat ttgaagcgca 31020ctcagcgtct gatccaagaa caaccatcct agcaacacgg cggtccccgg gctgagaaag 31080cccagtaagg aaacaactgt aggttcgagt cgcgagatcc cccggaacca aaggaagtag 31140gttaaacccg ctccgatcag gccgagccac gccaggccga gaacattggt tcctgtaggc 31200atcgggattg gcggatcaaa cactaaagct actggaacga gcagaagtcc tccggccgcc 31260agttgccagg cggtaaaggt gagcagaggc acgggaggtt gccacttgcg ggtcagcacg 31320gttccgaacg ccatggaaac cgcccccgcc aggcccgctg cgacgccgac aggatctagc 31380gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag ttccgcaaat agcccccagg 31440accgccatca atcgtatcgg gctacctagc agagcggcag agatgaacac gaccatcagc 31500ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca ggcggtagac cgaaataaac 31560aacaagctcc agaatagcga aatattaagt gcgccgagga tgaagatgcg catccaccag 31620attcccgttg gaatctgtcg gacgatcatc acgagcaata aacccgccgg caacgcccgc 31680agcagcatac cggcgacccc tcggcctcgc tgttcgggct ccacgaaaac gccggacaga 31740tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga agaccgacag cccaatgatc 31800tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca accgttcagc gaacgcctcc 31860atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca tctctggagc tttcttcagg 31920gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg ctccaagccg tcgaatctga 31980gccttaatca caattgtcaa ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg 32040tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa 32100atgccagtaa agcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc 32160gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca 32220actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc 32280cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat 32340agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg 32400tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg 32460acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt 32520gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct 32580cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga 32640acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc 32700acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct 32760tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg 32820tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg 32880gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca 32940acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct 33000tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct 33060cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag 33120ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg 33180actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct 33240cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat 33300tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct 33360gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg 33420tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga 33480ataccagcga ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact 33540tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cactcttcat 33600taaccgctat atcgaaaatt gcttgcggct tgttagaatt gccatgacgt acctcggtgt 33660cacgggtaag attaccgata aactggaact gattatggct catatcgaaa gtctccttga 33720gaaaggagac tctagtttag ctaaacattg gttccgctgt caagaacttt agcggctaaa 33780attttgcggg ccgcgaccaa aggtgcgagg ggcggcttcc gctgtgtaca accagatatt 33840tttcaccaac atccttcgtc tgctcgatga gcggggcatg acgaaacatg agctgtcgga 33900gagggcaggg gtttcaattt cgtttttatc agacttaacc aacggtaagg ccaacccctc 33960gttgaaggtg atggaggcca ttgccgacgc cctggaaact cccctacctc ttctcctgga 34020gtccaccgac cttgaccgcg aggcactcgc ggagattgcg ggtcatcctt tcaagagcag 34080cgtgccgccc ggatacgaac gcatcagtgt ggttttgccg tcacataagg cgtttatcgt 34140aaagaaatgg ggcgacgaca cccgaaaaaa gctgcgtgga aggctctgac gccaagggtt 34200agggcttgca cttccttctt tagccgctaa aacggcccct tctctgcggg ccgtcggctc 34260gcgcatcata tcgacatcct caacggaagc cgtgccgcga atggcatcgg gcgggtgcgc 34320tttgacagtt gttttctatc agaaccccta cgtcgtgcgg ttcgattagc tgtttgtctt 34380gcaggctaaa cactttcggt atatcgtttg cctgtgcgat aatgttgcta atgatttgtt 34440gcgtaggggt tactgaaaag tgagcgggaa agaagagttt cagaccatca aggagcgggc 34500caagcgcaag ctggaacgcg acatgggtgc ggacctgttg gccgcgctca acgacccgaa 34560aaccgttgaa gtcatgctca acgcggacgg caaggtgtgg cacgaacgcc ttggcgagcc 34620gatgcggtac atctgcgaca tgcggcccag ccagtcgcag gcgattatag aaacggtggc 34680cggattccac ggcaaagagg tcacgcggca ttcgcccatc ctggaaggcg agttcccctt 34740ggatggcagc cgctttgccg gccaattgcc gccggtcgtg gccgcgccaa cctttgcgat 34800ccgcaagcgc gcggtcgcca tcttcacgct ggaacagtac gtcgaggcgg gcatcatgac 34860ccgcgagcaa tacgaggtca ttaaaagcgc cgtcgcggcg catcgaaaca tcctcgtcat 34920tggcggtact ggctcgggca agaccacgct cgtcaacgcg atcatcaatg aaatggtcgc 34980cttcaacccg tctgagcgcg tcgtcatcat cgaggacacc ggcgaaatcc agtgcgccgc 35040agagaacgcc gtccaatacc acaccagcat cgacgtctcg atgacgctgc tgctcaagac 35100aacgctgcgt atgcgccccg accgcatcct ggtcggtgag gtacgtggcc ccgaagccct 35160tgatctgttg atggcctgga acaccgggca tgaaggaggt gccgccaccc tgcacgcaaa 35220caaccccaaa gcgggcctga gccggctcgc catgcttatc agcatgcacc cggattcacc 35280gaaacccatt gagccgctga ttggcgaggc ggttcatgtg gtcgtccata tcgccaggac 35340ccctagcggc cgtcgagtgc aagaaattct cgaagttctt ggttacgaga acggccagta 35400catcaccaaa accctgtaag gagtatttcc aatgacaacg gctgttccgt tccgtctgac 35460catgaatcgc ggcattttgt tctaccttgc cgtgttcttc gttctcgctc tcgcgttatc 35520cgcgcatccg gcgatggcct cggaaggcac cggcggcagc ttgccatatg agagctggct 35580gacgaacctg cgcaactccg taaccggccc ggtggccttc gcgctgtcca tcatcggcat 35640cgtcgtcgcc ggcggcgtgc tgatcttcgg cggcgaactc aacgccttct tccgaaccct 35700gatcttcctg gttctggtga tggcgctgct ggtcggcgcg cagaacgtga tgagcacctt 35760cttcggtcgt ggtgccgaaa tcgcggccct cggcaacggg gcgctgcacc aggtgcaagt 35820cgcggcggcg gatgccgtgc gtgcggtagc ggctggacgg ctcgcctaat catggctctg 35880cgcacgatcc ccatccgtcg cgcaggcaac cgagaaaacc tgttcatggg tggtgatcgt 35940gaactggtga tgttctcggg cctgatggcg tttgcgctga ttttcagcgc ccaagagctg 36000cgggccaccg tggtcggtct gatcctgtgg ttcggggcgc tctatgcgtt ccgaatcatg 36060gcgaaggccg atccgaagat gcggttcgtg tacctgcgtc accgccggta caagccgtat 36120tacccggccc gctcgacccc gttccgcgag aacaccaata gccaagggaa gcaataccga 36180tgatccaagc aattgcgatt gcaatcgcgg gcctcggcgc gcttctgttg ttcatcctct 36240ttgcccgcat ccgcgcggtc gatgccgaac tgaaactgaa aaagcatcgt tccaaggacg 36300ccggcctggc cgatctgctc aactacgccg ctgtcgtcga tgacggcgta atcgtgggca 36360agaacggcag ctttatggct gcctggctgt acaagggcga tgacaacgca agcagcaccg 36420accagcagcg cgaagtagtg tccgcccgca tcaaccaggc cctcgcgggc ctgggaagtg 36480ggtggatgat ccatgtggac gccgtgcggc gtcctgctcc gaactacgcg gagcggggcc 36540tgtcggcgtt ccctgaccgt ctgacggcag cgattgaaga agagcgctcg gtcttgcctt 36600gctcgtcggt gatgtacttc accagctccg cgaagtcgct cttcttgatg gagcgcatgg 36660ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa aagtcatggc 36720tctgccctcg ggcggaccac gcccatcatg accttgccaa gctcgtcctg cttctcttcg 36780atcttcgcca gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg 36840gtgagccaga gtttcagcag gccgcccagg cggcccaggt cgccattgat gcgggccagc 36900tcgcggacgt gctcatagtc cacgacgccc gtgattttgt agccctggcc gacggccagc 36960aggtaggccg acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg 37020tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca 37080gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga 37140ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg 37200ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct 37260acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa 37320atcgctataa tgaccccgaa gcagggttat gcagcggaaa agcgctgctt ccctgctgtt 37380ttgtggaata tctaccgact ggaaacaggc aaatgcagga aattactgaa ctgaggggac 37440aggcgagaga cgatgccaaa gagctacacc gacgagctgg ccgagtgggt tgaatcccgc 37500gcggccaaga agcgccggcg tgatgaggct gcggttgcgt tcctggcggt gagggcggat 37560gtcgaggcgg cgttagcgtc cggctatgcg ctcgtcacca tttgggagca catgcgggaa 37620acggggaagg tcaagttctc ctacgagacg ttccgctcgc acgccaggcg gcacatcaag 37680gccaagcccg ccgatgtgcc cgcaccgcag gccaaggctg cggaacccgc gccggcaccc 37740aagacgccgg agccacggcg gccgaagcag gggggcaagg ctgaaaagcc ggcccccgct 37800gcggccccga ccggcttcac cttcaaccca acaccggaca aaaaggatct actgtaatgg 37860cgaaaattca catggttttg cagggcaagg gcggggtcgg caagtcggcc atcgccgcga 37920tcattgcgca gtacaagatg gacaaggggc agacaccctt gtgcatcgac accgacccgg 37980tgaacgcgac gttcgagggc tacaaggccc tgaacgtccg ccggctgaac atcatggccg 38040gcgacgaaat taactcgcgc aacttcgaca ccctggtcga gctgattgcg ccgaccaagg 38100atgacgtggt gatcgacaac ggtgccagct cgttcgtgcc tctgtcgcat tacctcatca 38160gcaaccaggt gccggctctg ctgcaagaaa tggggcatga gctggtcatc cataccgtcg 38220tcaccggcgg ccaggctctc ctggacacgg tgagcggctt cgcccagctc gccagccagt 38280tcccggccga agcgcttttc gtggtctggc tgaacccgta ttgggggcct atcgagcatg 38340agggcaagag ctttgagcag atgaaggcgt acacggccaa caaggcccgc gtgtcgtcca 38400tcatccagat tccggccctc aaggaagaaa cctacggccg cgatttcagc gacatgctgc 38460aagagcggct gacgttcgac caggcgctgg ccgatgaatc gctcacgatc atgacgcggc 38520aacgcctcaa gatcgtgcgg cgcggcctgt

ttgaacagct cgacgcggcg gccgtgctat 38580gagcgaccag attgaagagc tgatccggga gattgcggcc aagcacggca tcgccgtcgg 38640ccgcgacgac ccggtgctga tcctgcatac catcaacgcc cggctcatgg ccgacagtgc 38700ggccaagcaa gaggaaatcc ttgccgcgtt caaggaagag ctggaaggga tcgcccatcg 38760ttggggcgag gacgccaagg ccaaagcgga gcggatgctg aacgcggccc tggcggccag 38820caaggacgca atggcgaagg taatgaagga cagcgccgcg caggcggccg aagcgatccg 38880cagggaaatc gacgacggcc ttggccgcca gctcgcggcc aaggtcgcgg acgcgcggcg 38940cgtggcgatg atgaacatga tcgccggcgg catggtgttg ttcgcggccg ccctggtggt 39000gtgggcctcg ttatgaatcg cagaggcgca gatgaaaaag cccggcgttg ccgggctttg 39060tttttgcgtt agctgggctt gtttgacagg cccaagctct gactgcgccc gcgctcgcgc 39120tcctgggcct gtttcttctc ctgctcctgc ttgcgcatca gggcctggtg ccgtcgggct 39180gcttcacgca tcgaatccca gtcgccggcc agctcgggat gctccgcgcg catcttgcgc 39240gtcgccagtt cctcgatctt gggcgcgtga atgcccatgc cttccttgat ttcgcgcacc 39300atgtccagcc gcgtgtgcag ggtctgcaag cgggcttgct gttgggcctg ctgctgctgc 39360caggcggcct ttgtacgcgg cagggacagc aagccggggg cattggactg tagctgctgc 39420aaacgcgcct gctgacggtc tacgagctgt tctaggcggt cctcgatgcg ctccacctgg 39480tcatgctttg cctgcacgta gagcgcaagg gtctgctggt aggtctgctc gatgggcgcg 39540gattctaaga gggcctgctg ttccgtctcg gcctcctggg ccgcctgtag caaatcctcg 39600ccgctgttgc cgctggactg ctttactgcc ggggactgct gttgccctgc tcgcgccgtc 39660gtcgcagttc ggcttgcccc cactcgattg actgcttcat ttcgagccgc agcgatgcga 39720tctcggattg cgtcaacgga cggggcagcg cggaggtgtc cggcttctcc ttgggtgagt 39780cggtcgatgc catagccaaa ggtttccttc caaaatgcgt ccattgctgg accgtgtttc 39840tcattgatgc ccgcaagcat cttcggcttg accgccaggt caagcgcgcc ttcatgggcg 39900gtcatgacgg acgccgccat gaccttgccg ccgttgttct cgatgtagcc gcgtaatgag 39960gcaatggtgc cgcccatcgt cagcgtgtca tcgacaacga tgtacttctg gccggggatc 40020acctccccct cgaaagtcgg gttgaacgcc aggcgatgat ctgaaccggc tccggttcgg 40080gcgaccttct cccgctgcac aatgtccgtt tcgacctcaa ggccaaggcg gtcggccaga 40140acgaccgcca tcatggccgg aatcttgttg ttccccgccg cctcgacggc gaggactgga 40200acgatgcggg gcttgtcgtc gccgatcagc gtcttgagct gggcaacagt gtcgtccgaa 40260atcaggcgct cgaccaaatt aagcgccgct tccgcgtcgc cctgcttcgc agcctggtat 40320tcaggctcgt tggtcaaaga accaaggtcg ccgttgcgaa ccaccttcgg gaagtctccc 40380cacggtgcgc gctcggctct gctgtagctg ctcaagacgc ctcccttttt agccgctaaa 40440actctaacga gtgcgcccgc gactcaactt gacgctttcg gcacttacct gtgccttgcc 40500acttgcgtca taggtgatgc ttttcgcact cccgatttca ggtactttat cgaaatctga 40560ccgggcgtgc attacaaagt tcttccccac ctgttggtaa atgctgccgc tatctgcgtg 40620gacgatgctg ccgtcgtggc gctgcgactt atcggccttt tgggccatat agatgttgta 40680aatgccaggt ttcagggccc cggctttatc taccttctgg ttcgtccatg cgccttggtt 40740ctcggtctgg acaattcttt gcccattcat gaccaggagg cggtgtttca ttgggtgact 40800cctgacggtt gcctctggtg ttaaacgtgt cctggtcgct tgccggctaa aaaaaagccg 40860acctcggcag ttcgaggccg gctttcccta gagccgggcg cgtcaaggtt gttccatcta 40920ttttagtgaa ctgcgttcga tttatcagtt actttcctcc cgctttgtgt ttcctcccac 40980tcgtttccgc gtctagccga cccctcaaca tagcggcctc ttcttgggct gcctttgcct 41040cttgccgcgc ttcgtcacgc tcggcttgca ccgtcgtaaa gcgctcggcc tgcctggccg 41100cctcttgcgc cgccaacttc ctttgctcct ggtgggcctc ggcgtcggcc tgcgccttcg 41160ctttcaccgc tgccaactcc gtgcgcaaac tctccgcttc gcgcctggtg gcgtcgcgct 41220cgccgcgaag cgcctgcatt tcctggttgg ccgcgtccag ggtcttgcgg ctctcttctt 41280tgaatgcgcg ggcgtcctgg tgagcgtagt ccagctcggc gcgcagctcc tgcgctcgac 41340gctccacctc gtcggcccgc tgcgtcgcca gcgcggcccg ctgctcggct cctgccaggg 41400cggtgcgtgc ttcggccagg gcttgccgct ggcgtgcggc cagctcggcc gcctcggcgg 41460cctgctgctc tagcaatgta acgcgcgcct gggcttcttc cagctcgcgg gcctgcgcct 41520cgaaggcgtc ggccagctcc ccgcgcacgg cttccaactc gttgcgctca cgatcccagc 41580cggcttgcgc tgcctgcaac gattcattgg caagggcctg ggcggcttgc cagagggcgg 41640ccacggcctg gttgccggcc tgctgcaccg cgtccggcac ctggactgcc agcggggcgg 41700cctgcgccgt gcgctggcgt cgccattcgc gcatgccggc gctggcgtcg ttcatgttga 41760cgcgggcggc cttacgcact gcatccacgg tcgggaagtt ctcccggtcg ccttgctcga 41820acagctcgtc cgcagccgca aaaatgcggt cgcgcgtctc tttgttcagt tccatgttgg 41880ctccggtaat tggtaagaat aataatactc ttacctacct tatcagcgca agagtttagc 41940tgaacagttc tcgacttaac ggcaggtttt ttagcggctg aagggcaggc aaaaaaagcc 42000ccgcacggtc ggcgggggca aagggtcagc gggaagggga ttagcgggcg tcgggcttct 42060tcatgcgtcg gggccgcgct tcttgggatg gagcacgacg aagcgcgcac gcgcatcgtc 42120ctcggcccta tcggcccgcg tcgcggtcag gaacttgtcg cgcgctaggt cctccctggt 42180gggcaccagg ggcatgaact cggcctgctc gatgtaggtc cactccatga ccgcatcgca 42240gtcgaggccg cgttccttca ccgtctcttg caggtcgcgg tacgcccgct cgttgagcgg 42300ctggtaacgg gccaattggt cgtaaatggc tgtcggccat gagcggcctt tcctgttgag 42360ccagcagccg acgacgaagc cggcaatgca ggcccctggc acaaccaggc cgacgccggg 42420ggcaggggat ggcagcagct cgccaaccag gaaccccgcc gcgatgatgc cgatgccggt 42480caaccagccc ttgaaactat ccggccccga aacacccctg cgcattgcct ggatgctgcg 42540ccggatagct tgcaacatca ggagccgttt cttttgttcg tcagtcatgg tccgccctca 42600ccagttgttc gtatcggtgt cggacgaact gaaatcgcaa gagctgccgg tatcggtcca 42660gccgctgtcc gtgtcgctgc tgccgaagca cggcgagggg tccgcgaacg ccgcagacgg 42720cgtatccggc cgcagcgcat cgcccagcat ggccccggtc agcgagccgc cggccaggta 42780gcccagcatg gtgctgttgg tcgccccggc caccagggcc gacgtgacga aatcgccgtc 42840attccctctg gattgttcgc tgctcggcgg ggcagtgcgc cgcgccggcg gcgtcgtgga 42900tggctcgggt tggctggcct gcgacggccg gcgaaaggtg cgcagcagct cgttatcgac 42960cggctgcggc gtcggggccg ccgccttgcg ctgcggtcgg tgttccttct tcggctcgcg 43020cagcttgaac agcatgatcg cggaaaccag cagcaacgcc gcgcctacgc ctcccgcgat 43080gtagaacagc atcggattca ttcttcggtc ctccttgtag cggaaccgtt gtctgtgcgg 43140cgcgggtggc ccgcgccgct gtctttgggg atcagccctc gatgagcgcg accagtttca 43200cgtcggcaag gttcgcctcg aactcctggc cgtcgtcctc gtacttcaac caggcatagc 43260cttccgccgg cggccgacgg ttgaggataa ggcgggcagg gcgctcgtcg tgctcgacct 43320ggacgatggc ctttttcagc ttgtccgggt ccggctcctt cgcgcccttt tccttggcgt 43380ccttaccgtc ctggtcgccg tcctcgccgt cctggccgtc gccggcctcc gcgtcacgct 43440cggcatcagt ctggccgttg aaggcatcga cggtgttggg atcgcggccc ttctcgtcca 43500ggaactcgcg cagcagcttg accgtgccgc gcgtgatttc ctgggtgtcg tcgtcaagcc 43560acgcctcgac ttcctccggg cgcttcttga aggccgtcac cagctcgttc accacggtca 43620cgtcgcgcac gcggccggtg ttgaacgcat cggcgatctt ctccggcagg tccagcagcg 43680tgacgtgctg ggtgatgaac gccggcgact tgccgatttc cttggcgata tcgcctttct 43740tcttgccctt cgccagctcg cggccaatga agtcggcaat ttcgcgcggg gtcagctcgt 43800tgcgttgcag gttctcgata acctggtcgg cttcgttgta gtcgttgtcg atgaacgccg 43860ggatggactt cttgccggcc cacttcgagc cacggtagcg gcgggcgccg tgattgatga 43920tatagcggcc cggctgctcc tggttctcgc gcaccgaaat gggtgacttc accccgcgct 43980ctttgatcgt ggcaccgatt tccgcgatgc tctccgggga aaagccgggg ttgtcggccg 44040tccgcggctg atgcggatct tcgtcgatca ggtccaggtc cagctcgata gggccggaac 44100cgccctgaga cgccgcagga gcgtccagga ggctcgacag gtcgccgatg ctatccaacc 44160ccaggccgga cggctgcgcc gcgcctgcgg cttcctgagc ggccgcagcg gtgtttttct 44220tggtggtctt ggcttgagcc gcagtcattg ggaaatctcc atcttcgtga acacgtaatc 44280agccagggcg cgaacctctt tcgatgcctt gcgcgcggcc gttttcttga tcttccagac 44340cggcacaccg gatgcgaggg catcggcgat gctgctgcgc aggccaacgg tggccggaat 44400catcatcttg gggtacgcgg ccagcagctc ggcttggtgg cgcgcgtggc gcggattccg 44460cgcatcgacc ttgctgggca ccatgccaag gaattgcagc ttggcgttct tctggcgcac 44520gttcgcaatg gtcgtgacca tcttcttgat gccctggatg ctgtacgcct caagctcgat 44580gggggacagc acatagtcgg ccgcgaagag ggcggccgcc aggccgacgc caagggtcgg 44640ggccgtgtcg atcaggcaca cgtcgaagcc ttggttcgcc agggccttga tgttcgcccc 44700gaacagctcg cgggcgtcgt ccagcgacag ccgttcggcg ttcgccagta ccgggttgga 44760ctcgatgagg gcgaggcgcg cggcctggcc gtcgccggct gcgggtgcgg tttcggtcca 44820gccgccggca gggacagcgc cgaacagctt gcttgcatgc aggccggtag caaagtcctt 44880gagcgtgtag gacgcattgc cctgggggtc caggtcgatc acggcaaccc gcaagccgcg 44940ctcgaaaaag tcgaaggcaa gatgcacaag ggtcgaagtc ttgccgacgc cgcctttctg 45000gttggccgtg accaaagttt tcatcgtttg gtttcctgtt ttttcttggc gtccgcttcc 45060cacttccgga cgatgtacgc ctgatgttcc ggcagaaccg ccgttacccg cgcgtacccc 45120tcgggcaagt tcttgtcctc gaacgcggcc cacacgcgat gcaccgcttg cgacactgcg 45180cccctggtca gtcccagcga cgttgcgaac gtcgcctgtg gcttcccatc gactaagacg 45240ccccgcgcta tctcgatggt ctgctgcccc acttccagcc cctggatcgc ctcctggaac 45300tggctttcgg taagccgttt cttcatggat aacacccata atttgctccg cgccttggtt 45360gaacatagcg gtgacagccg ccagcacatg agagaagttt agctaaacat ttctcgcacg 45420tcaacacctt tagccgctaa aactcgtcct tggcgtaaca aaacaaaagc ccggaaaccg 45480ggctttcgtc tcttgccgct tatggctctg cacccggctc catcaccaac aggtcgcgca 45540cgcgcttcac tcggttgcgg atcgacactg ccagcccaac aaagccggtt gccgccgccg 45600ccaggatcgc gccgatgatg ccggccacac cggccatcgc ccaccaggtc gccgccttcc 45660ggttccattc ctgctggtac tgcttcgcaa tgctggacct cggctcacca taggctgacc 45720gctcgatggc gtatgccgct tctccccttg gcgtaaaacc cagcgccgca ggcggcattg 45780ccatgctgcc cgccgctttc ccgaccacga cgcgcgcacc aggcttgcgg tccagacctt 45840cggccacggc gagctgcgca aggacataat cagccgccga cttggctcca cgcgcctcga 45900tcagctcttg cactcgcgcg aaatccttgg cctccacggc cgccatgaat cgcgcacgcg 45960gcgaaggctc cgcagggccg gcgtcgtgat cgccgccgag aatgcccttc accaagttcg 46020acgacacgaa aatcatgctg acggctatca ccatcatgca gacggatcgc acgaacccgc 46080tgaattgaac acgagcacgg cacccgcgac cactatgcca agaatgccca aggtaaaaat 46140tgccggcccc gccatgaagt ccgtgaatgc cccgacggcc gaagtgaagg gcaggccgcc 46200acccaggccg ccgccctcac tgcccggcac ctggtcgctg aatgtcgatg ccagcacctg 46260cggcacgtca atgcttccgg gcgtcgcgct cgggctgatc gcccatcccg ttactgcccc 46320gatcccggca atggcaagga ctgccagcgc tgccattttt ggggtgaggc cgttcgcggc 46380cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag 46440aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa 46500aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga 46560aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg 46620tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc 46680ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc 46740ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg 46800ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc 46860cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt 46920tttccgcgag gtatccacaa cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg 46980tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc 47040cggtgagcgt cggaaaggcg ctggaagccc cgtagcgacg cggagagggg cgagacaagc 47100caagggcgca ggctcgatgc gcagcacgac atagccggtt ctcgcaagga cgagaatttc 47160cctgcggtgc ccctcaagtg tcaatgaaag tttccaacgc gagccattcg cgagagcctt 47220gagtccacgc tagatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg 47280ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc 47340aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc 47400acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac 47460aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgac 475051448232DNAartificial sequencePHP16973 (ZmCAS1BamProMS45/35SPAT) 14tctagagctc gttcctcgag gaacggtacc tgcggggaag cttacaataa tgtgtgttgt 60taagtcttgt tgcctgtcat cgtctgactg actttcgtca taaatcccgg cctccgtaac 120ccagctttgg gcaagctcac ggatttgatc cggcggaacg ggaatatcga gatgccgggc 180tgaacgctgc agttccagct ttccctttcg ggacaggtac tccagctgat tgattatctg 240ctgaagggtc ttggttccac ctcctggcac aatgcgaatg attacttgag cgcgatcggg 300catccaattt tctcccgtca ggtgcgtggt caagtgctac aaggcacctt tcagtaacga 360gcgaccgtcg atccgtcgcc gggatacgga caaaatggag cgcagtagtc catcgagggc 420ggcgaaagcc tcgccaaaag caatacgttc atctcgcaca gcctccagat ccgatcgagg 480gtcttcggcg taggcagata gaagcatgga tacattgctt gagagtattc cgatggactg 540aagtatggct tccatctttt ctcgtgtgtc tgcatctatt tcgagaaagc ccccgatgcg 600gcgcaccgca acgcgaattg ccatactatc cgaaagtccc agcaggcgcg cttgatagga 660aaaggtttca tactcggccg atcgcagacg ggcactcacg accttgaacc cttcaacttt 720cagggatcga tgctggttga tggtagtctc actcgacgtg gctctggtgt gttttgacat 780agcttcctcc aaagaaagcg gaaggtctgg atactccagc acgaaatgtg cccgggtaga 840cggatggaag tctagccctg ctcaatatga aatcaacagt acatttacag tcaatactga 900atatacttgc tacatttgca attgtcttat aacgaatgtg aaataaaaat agtgtaacaa 960cgcttttact catcgataat cacaaaaaca tttatacgaa caaaaataca aatgcactcc 1020ggtttcacag gataggcggg atcagaatat gcaacttttg acgttttgtt ctttcaaagg 1080gggtgctggc aaaaccaccg cactcatggg cctttgcgct gctttggcaa atgacggtaa 1140acgagtggcc ctctttgatg ccgacgaaaa ccggcctctg acgcgatgga gagaaaacgc 1200cttacaaagc agtactggga tcctcgctgt gaagtctatt ccgccgacga aatgcccctt 1260cttgaagcag cctatgaaaa tgccgagctc gaaggatttg attatgcgtt ggccgatacg 1320cgtggcggct cgagcgagct caacaacaca atcatcgcta gctcaaacct gcttctgatc 1380cccaccatgc taacgccgct cgacatcgat gaggcactat ctacctaccg ctacgtcatc 1440gagctgctgt tgagtgaaaa tttggcaatt cctacagctg ttttgcgcca acgcgtcccg 1500gtcggccgat tgacaacatc gcaacgcagg atgtcagaga cgctagagag ccttccagtt 1560gtaccgtctc ccatgcatga aagagatgca tttgccgcga tgaaagaacg cggcatgttg 1620catcttacat tactaaacac gggaactgat ccgacgatgc gcctcataga gaggaatctt 1680cggattgcga tggaggaagt cgtggtcatt tcgaaactga tcagcaaaat cttggaggct 1740tgaagatggc aattcgcaag cccgcattgt cggtcggcga agcacggcgg cttgctggtg 1800ctcgacccga gatccaccat cccaacccga cacttgttcc ccagaagctg gacctccagc 1860acttgcctga aaaagccgac gagaaagacc agcaacgtga gcctctcgtc gccgatcaca 1920tttacagtcc cgatcgacaa cttaagctaa ctgtggatgc ccttagtcca cctccgtccc 1980cgaaaaagct ccaggttttt ctttcagcgc gaccgcccgc gcctcaagtg tcgaaaacat 2040atgacaacct cgttcggcaa tacagtccct cgaagtcgct acaaatgatt ttaaggcgcg 2100cgttggacga tttcgaaagc atgctggcag atggatcatt tcgcgtggcc ccgaaaagtt 2160atccgatccc ttcaactaca gaaaaatccg ttctcgttca gacctcacgc atgttcccgg 2220ttgcgttgct cgaggtcgct cgaagtcatt ttgatccgtt ggggttggag accgctcgag 2280ctttcggcca caagctggct accgccgcgc tcgcgtcatt ctttgctgga gagaagccat 2340cgagcaattg gtgaagaggg acctatcgga acccctcacc aaatattgag tgtaggtttg 2400aggccgctgg ccgcgtcctc agtcaccttt tgagccagat aattaagagc caaatgcaat 2460tggctcaggc tgccatcgtc cccccgtgcg aaacctgcac gtccgcgtca aagaaataac 2520cggcacctct tgctgttttt atcagttgag ggcttgacgg atccgcctca agtttgcggc 2580gcagccgcaa aatgagaaca tctatactcc tgtcgtaaac ctcctcgtcg cgtactcgac 2640tggcaatgag aagttgctcg cgcgatagaa cgtcgcgggg tttctctaaa aacgcgagga 2700gaagattgaa ctcacctgcc gtaagtttca cctcaccgcc agcttcggac atcaagcgac 2760gttgcctgag attaagtgtc cagtcagtaa aacaaaaaga ccgtcggtct ttggagcgga 2820caacgttggg gcgcacgcgc aaggcaaccc gaatgcgtgc aagaaactct ctcgtactaa 2880acggcttagc gataaaatca cttgctccta gctcgagtgc aacaacttta tccgtctcct 2940caaggcggtc gccactgata attatgattg gaatatcaga ctttgccgcc agatttcgaa 3000cgatctcaag cccatcttca cgacctaaat ttagatcaac aaccacgaca tcgaccgtcg 3060cggaagagag tactctagtg aactgggtgc tgtcggctac cgcggtcact ttgaaggcgt 3120ggatcgtaag gtattcgata ataagatgcc gcatagcgac atcgtcatcg ataagaagaa 3180cgtgtttcaa cggctcacct ttcaatctaa aatctgaacc cttgttcaca gcgcttgaga 3240aattttcacg tgaaggatgt acaatcatct ccagctaaat gggcagttcg tcagaattgc 3300ggctgaccgc ggatgacgaa aatgcgaacc aagtatttca attttatgac aaaagttctc 3360aatcgttgtt acaagtgaaa cgcttcgagg ttacagctac tattgattaa ggagatcgcc 3420tatggtctcg ccccggcgtc gtgcgtccgc cgcgagccag atctcgccta cttcataaac 3480gtcctcatag gcacggaatg gaatgatgac atcgatcgcc gtagagagca tgtcaatcag 3540tgtgcgatct tccaagctag caccttgggc gctacttttg acaagggaaa acagtttctt 3600gaatccttgg attggattcg cgccgtgtat tgttgaaatc gatcccggat gtcccgagac 3660gacttcactc agataagccc atgctgcatc gtcgcgcatc tcgccaagca atatccggtc 3720cggccgcata cgcagacttg cttggagcaa gtgctcggcg ctcacagcac ccagcccagc 3780accgttcttg gagtagagta gtctaacatg attatcgtgt ggaatgacga gttcgagcgt 3840atcttctatg gtgattagcc tttcctgggg ggggatggcg ctgatcaagg tcttgctcat 3900tgttgtcttg ccgcttccgg tagggccaca tagcaacatc gtcagtcggc tgacgacgca 3960tgcgtgcaga aacgcttcca aatccccgtt gtcaaaatgc tgaaggatag cttcatcatc 4020ctgattttgg cgtttccttc gtgtctgcca ctggttccac ctcgaagcat cataacggga 4080ggagacttct ttaagaccag aaacacgcga gcttggccgt cgaatggtca agctgacggt 4140gcccgaggga acggtcggcg gcagacagat ttgtagtcgt tcaccaccag gaagttcagt 4200ggcgcagagg gggttacgtg gtccgacatc ctgctttctc agcgcgcccg ctaaaatagc 4260gatatcttca agatcatcat aagagacggg caaaggcatc ttggtaaaaa tgccggcttg 4320gcgcacaaat gcctctccag gtcgattgat cgcaatttct tcagtcttcg ggtcatcgag 4380ccattccaaa atcggcttca gaagaaagcg tagttgcgga tccacttcca tttacaatgt 4440atcctatctc taagcggaaa tttgaattca ttaagagcgg cggttcctcc cccgcgtggc 4500gccgccagtc aggcggagct ggtaaacacc aaagaaatcg aggtcccgtg ctacgaaaat 4560ggaaacggtg tcaccctgat tcttcttcag ggttggcggt atgttgatgg ttgccttaag 4620ggctgtctca gttgtctgct caccgttatt ttgaaagctg ttgaagctca tcccgccacc 4680cgagctgccg gcgtaggtgc tagctgcctg gaaggcgcct tgaacaacac tcaagagcat 4740agctccgcta aaacgctgcc agaagtggct gtcgaccgag cccggcaatc ctgagcgacc 4800gagttcgtcc gcgcttggcg atgttaacga gatcatcgca tggtcaggtg tctcggcgcg 4860atcccacaac acaaaaacgc gcccatctcc ctgttgcaag ccacgctgta tttcgccaac 4920aacggtggtg ccacgatcaa gaagcacgat attgttcgtt gttccacgaa tatcctgagg 4980caagacacac tttacatagc ctgccaaatt tgtgtcgatt gcggtttgca agatgcacgg 5040aattattgtc ccttgcgtta ccataaaatc ggggtgcggc aagagcgtgg cgctgctggg 5100ctgcagctcg gtgggtttca tacgtatcga caaatcgttc tcgccggaca cttcgccatt 5160cggcaaggag ttgtcgtcac gcttgccttc ttgtcttcgg cccgtgtcgc cctgaatggc 5220gcgtttgctg accccttgat cgccgctgct atatgcaaaa atcggtgttt cttccggccg 5280tggctcatgc cgctccggtt cgcccctcgg cggtagagga gcagcaggct gaacagcctc 5340ttgaaccgct ggaggatccg gcggcacctc aatcggagct ggatgaaatg gcttggtgtt 5400tgttgcgatc aaagttgacg gcgatgcgtt ctcattcacc ttcttttggc gcccacctag 5460ccaaatgagg cttaatgata acgcgagaac gacacctccg acgatcaatt tctgagaccc 5520cgaaagacgc cggcgatgtt tgtcggagac cagggatcca gatgcatcaa cctcatgtgc 5580cgcttgctga ctatcgttat tcatcccttc gcccccttca ggacgcgttt cacatcgggc 5640ctcaccgtgc ccgtttgcgg cctttggcca acgggatcgt aagcggtgtt ccagatacat 5700agtactgtgt ggccatccct cagacgccaa cctcgggaaa ccgaagaaat ctcgacatcg 5760ctccctttaa ctgaatagtt ggcaacagct tccttgccat caggattgat ggtgtagatg 5820gagggtatgc gtacattgcc cggaaagtgg aataccgtcg taaatccatt gtcgaagact 5880tcgagtggca acagcgaacg atcgccttgg gcgacgtagt gccaattact gtccgccgca 5940ccaagggctg tgacaggctg atccaataaa ttctcagctt tccgttgata ttgtgcttcc 6000gcgtgtagtc tgtccacaac

agccttctgt tgtgcctccc ttcgccgagc cgccgcatcg 6060tcggcggggt aggcgaattg gacgctgtaa tagagatcgg gctgctcttt atcgaggtgg 6120gacagagtct tggaacttat actgaaaaca taacggcgca tcccggagtc gcttgcggtt 6180agcacgatta ctggctgagg cgtgaggacc tggcttgcct tgaaaaatag ataatttccc 6240cgcggtaggg ctgctagatc tttgctattt gaaacggcaa ccgctgtcac cgtttcgttc 6300gtggcgaatg ttacgaccaa agtagctcca accgccgtcg agaggcgcac cacttgatcg 6360ggattgtaag ccaaataacg catgcgcgga tctagcttgc ccgccattgg agtgtcttca 6420gcctccgcac cagtcgcagc ggcaaataaa catgctaaaa tgaaaagtgc ttttctgatc 6480atggttcgct gtggcctacg tttgaaacgg tatcttccga tgtctgatag gaggtgacaa 6540ccagacctgc cgggttggtt agtctcaatc tgccgggcaa gctggtcacc ttttcgtagc 6600gaactgtcgc ggtccacgta ctcaccacag gcattttgcc gtcaacgacg agggtccttt 6660tatagcgaat ttgctgcgtg cttggagtta catcatttga agcgatgtgc tcgacctcca 6720ccctgccgcg tttgccaaga atgacttgag gcgaactggg attgggatag ttgaagaatt 6780gctggtaatc ctggcgcact gttggggcac tgaagttcga taccaggtcg taggcgtact 6840gagcggtgtc ggcatcataa ctctcgcgca ggcgaacgta ctcccacaat gaggcgttaa 6900cgacggcctc ctcttgagtt gcaggcaatc gcgagacaga cacctcgctg tcaacggtgc 6960cgtccggccg tatccataga tatacgggca caagcctgct caacggcacc attgtggcta 7020tagcgaacgc ttgagcaaca tttcccaaaa tcgcgatagc tgcgacagct gcaatgagtt 7080tggagagacg tcgcgccgat ttcgctcgcg cggtttgaaa ggcttctact tccttatagt 7140gctcggcaag gctttcgcgc gccactagca tggcatattc aggccccgtc atagcgtcca 7200cccgaattgc cgagctgaag atctgacgga gtaggctgcc atcgccccac attcagcggg 7260aagatcgggc ctttgcagct cgctaatgtg tcgtttgtct ggcagccgct caaagcgaca 7320actaggcaca gcaggcaata cttcatagaa ttctccattg aggcgaattt ttgcgcgacc 7380tagcctcgct caacctgagc gaagcgacgg tacaagctgc tggcagattg ggttgcgccg 7440ctccagtaac tgcctccaat gttgccggcg atcgccggca aagcgacaat gagcgcatcc 7500cctgtcagaa aaaacatatc gagttcgtaa agaccaatga tcttggccgc ggtcgtaccg 7560gcgaaggtga ttacaccaag cataagggtg agcgcagtcg cttcggttag gatgacgatc 7620gttgccacga ggtttaagag gagaagcaag agaccgtagg tgataagttg cccgatccac 7680ttagctgcga tgtcccgcgt gcgatcaaaa atatatccga cgaggatcag aggcccgatc 7740gcgagaagca ctttcgtgag aattccaacg gcgtcgtaaa ctccgaaggc agaccagagc 7800gtgccgtaaa ggacccactg tgccccttgg aaagcaagga tgtcctggtc gttcatcgga 7860ccgatttcgg atgcgatttt ctgaaaaacg gcctgggtca cggcgaacat tgtatccaac 7920tgtgccggaa cagtctgcag aggcaagccg gttacactaa actgctgaac aaagtttggg 7980accgtctttt cgaagatgga aaccacatag tcttggtagt tagcctgccc aacaattaga 8040gcaacaacga tggtgaccgt gatcacccga gtgataccgc tacgggtatc gacttcgccg 8100cgtatgacta aaataccctg aacaataatc caaagagtga cacaggcgat caatggcgca 8160ctcaccgcct cctggatagt ctcaagcatc gagtccaagc ctgtcgtgaa ggctacatcg 8220aagatcgtat gaatggccgt aaacggcgcc ggaatcgtga aattcatcga ttggacctga 8280acttgactgg tttgtcgcat aatgttggat aaaatgagct cgcattcggc gaggatgcgg 8340gcggatgaac aaatcgccca gccttagggg agggcaccaa agatgacagc ggtcttttga 8400tgctccttgc gttgagcggc cgcctcttcc gcctcgtgaa ggccggcctg cgcggtagtc 8460atcgttaata ggcttgtcgc ctgtacattt tgaatcattg cgtcatggat ctgcttgaga 8520agcaaaccat tggtcacggt tgcctgcatg atattgcgag atcgggaaag ctgagcagac 8580gtatcagcat tcgccgtcaa gcgtttgtcc atcgtttcca gattgtcagc cgcaatgcca 8640gcgctgtttg cggaaccggt gatctgcgat cgcaacaggt ccgcttcagc atcactaccc 8700acgactgcac gatctgtatc gctggtgatc gcacgtgccg tggtcgacat tggcattcgc 8760ggcgaaaaca tttcattgtc taggtccttc gtcgaaggat actgattttt ctggttgagc 8820gaagtcagta gtccagtaac gccgtaggcc gacgtcaaca tcgtaaccat cgctatagtc 8880tgagtgagat tctccgcagt cgcgagcgca gtcgcgagcg tctcagcctc cgttgccggg 8940tcgctaacaa caaactgcgc ccgcgcgggc tgaatatata gaaagctgca ggtcaaaact 9000gttgcaataa gttgcgtcgt cttcatcgtt tcctacctta tcaatcttct gcctcgtggt 9060gacgggccat gaattcgctg agccagccag atgagttgcc ttcttgtgcc tcgcgtagtc 9120gagttgcaaa gcgcaccgtg ttggcacgcc ccgaaagcac ggcgacatat tcacgcatat 9180cccgcagatc aaattcgcag atgacgcttc cactttctcg tttaagaaga aacttacggc 9240tgccgaccgt catgtcttca cggatcgcct gaaattcctt ttcggtacat ttcagtccat 9300cgacataagc cgatcgatct gcggttggtg atggatagaa aatcttcgtc atacattgcg 9360caaccaagct ggctcctagc ggcgattcca gaacatgctc tggttgctgc gttgccagta 9420ttagcatccc gttgtttttt cgaacggtca ggaggaattt gtcgacgaca gtcgaaaatt 9480tagggtttaa caaataggcg cgaaactcat cgcagctcat cacaaaacgg cggccgtcga 9540tcatggctcc aatccgatgc aggagatatg ctgcagcggg agcgcatact tcctcgtatt 9600cgagaagatg cgtcatgtcg aagccggtaa tcgacggatc taactttact tcgtcaactt 9660cgccgtcaaa tgcccagcca agcgcatggc cccggcacca gcgttggagc cgcgctcctg 9720cgccttcggc gggcccatgc aacaaaaatt cacgtaaccc cgcgattgaa cgcatttgtg 9780gatcaaacga gagctgacga tggataccac ggaccagacg gcggttctct tccggagaaa 9840tcccaccccg accatcactc tcgatgagag ccacgatcca ttcgcgcaga aaatcgtgtg 9900aggctgctgt gttttctagg ccacgcaacg gcgccaaccc gctgggtgtg cctctgtgaa 9960gtgccaaata tgttcctcct gtggcgcgaa ccagcaattc gccaccccgg tccttgtcaa 10020agaacacgac cgtacctgca cggtcgacca tgctctgttc gagcatggct agaacaaaca 10080tcatgagcgt cgtcttaccc ctcccgatag gcccgaatat tgccgtcatg ccaacatcgt 10140gctcatgcgg gatatagtcg aaaggcgttc cgccattggt acgaaatcgg gcaatcgcgt 10200tgccccagtg gcctgagctg gcgccctctg gaaagttttc gaaagagaca aaccctgcga 10260aattgcgtga agtgattgcg ccagggcgtg tgcgccactt aaaattcccc ggcaattggg 10320accaataggc cgcttccata ccaatacctt cttggacaac cacggcacct gcatccgcca 10380ttcgtgtccg agcccgcgcg cccctgtccc caagactatt gagatcgtct gcatagacgc 10440aaaggctcaa atgatgtgag cccataacga attcgttgct cgcaagtgcg tcctcagcct 10500cggataattt gccgatttga gtcacggctt tatcgccgga actcagcatc tggctcgatt 10560tgaggctaag tttcgcgtgc gcttgcgggc gagtcaggaa cgaaaaactc tgcgtgagaa 10620caagtggaaa atcgagggat agcagcgcgt tgagcatgcc cggccgtgtt tttgcagggt 10680attcgcgaaa cgaatagatg gatccaacgt aactgtcttt tggcgttctg atctcgagtc 10740ctcgcttgcc gcaaatgact ctgtcggtat aaatcgaagc gccgagtgag ccgctgacga 10800ccggaaccgg tgtgaaccga ccagtcatga tcaaccgtag cgcttcgcca atttcggtga 10860agagcacacc ctgcttctcg cggatgccaa gacgatgcag gccatacgct ttaagagagc 10920cagcgacaac atgccaaaga tcttccatgt tcctgatctg gcccgtgaga tcgttttccc 10980tttttccgct tagcttggtg aacctcctct ttaccttccc taaagccgcc tgtgggtaga 11040caatcaacgt aaggaagtgt tcattgcgga ggagttggcc ggagagcacg cgctgttcaa 11100aagcttcgtt caggctagcg gcgaaaacac tacggaagtg tcgcggcgcc gatgatggca 11160cgtcggcatg acgtacgagg tgagcatata ttgacacatg atcatcagcg atattgcgca 11220acagcgtgtt gaacgcacga caacgcgcat tgcgcatttc agtttcctca agctcgaatg 11280caacgccatc aattctcgca atggtcatga tcgatccgtc ttcaagaagg acgatatggt 11340cgctgaggtg gccaatataa gggagataga tctcaccgga tctttcggtc gttccactcg 11400cgccgagcat cacaccattc ctctccctcg tgggggaacc ctaattggat ttgggctaac 11460agtagcgccc ccccaaactg cactatcaat gcttcttccc gcggtccgca aaaatagcag 11520gacgacgctc gccgcattgt agtctcgctc cacgatgagc cgggctgcaa accataacgg 11580cacgagaacg acttcgtaga gcgggttctg aacgataacg atgacaaagc cggcgaacat 11640catgaataac cctgccaatg tcagtggcac cccaagaaac aatgcgggcc gtgtggctgc 11700gaggtaaagg gtcgattctt ccaaacgatc agccatcaac taccgccagt gagcgtttgg 11760ccgaggaagc tcgccccaaa catgataaca atgccgccga cgacgccggc aaccagccca 11820agcgaagccc gcccgaacat ccaggagatc ccgatagcga caatgccgag aacagcgagt 11880gactggccga acggaccaag gataaacgtg catatattgt taaccattgt ggcggggtca 11940gtgccgccac ccgcagattg cgctgcggcg ggtccggatg aggaaatgct ccatgcaatt 12000gcaccgcaca agcttggggc gcagctcgat atcacgcgca tcatcgcatt cgagagcgag 12060aggcgattta gatgtaaacg gtatctctca aagcatcgca tcaatgcgca cctccttagt 12120ataagtcgaa taagacttga ttgtcgtctg cggatttgcc gttgtcctgg tgtggcggtg 12180gcggagcgat taaaccgcca gcgccatcct cctgcgagcg gcgctgatat gacccccaaa 12240catcccacgt ctcttcggat tttagcgcct cgtgatcgtc ttttggaggc tcgattaacg 12300cgggcaccag cgattgagca gctgtttcaa cttttcgcac gtagccgttt gcaaaaccgc 12360cgatgaaatt accggtgttg taagcggaga tcgcccgacg aagcgcaaat tgcttctcgt 12420caatcgtttc gccgcctgca taacgacttt tcagcatgtt tgcagcggca gataatgatg 12480tgcacgcctg gagcgcaccg tcaggtgtca gaccgagcat agaaaaattt cgagagttta 12540tttgcatgag gccaacatcc agcgaatgcc gtgcatcgag acggtgcctg acgacttggg 12600ttgcttggct gtgatcttgc cagtgaagcg tttcgccggt cgtgttgtca tgaatcgcta 12660aaggatcaaa gcgactctcc accttagcta tcgccgcaag cgtagatgtc gcaactgatg 12720gggcacactt gcgagcaaca tggtcaaact cagcagatga gagtggcgtg gcaaggctcg 12780acgaacagaa ggagaccatc aaggcaagag aaagcgaccc cgatctctta agcatacctt 12840atctccttag ctcgcaacta acaccgcctc tcccgttgga agaagtgcgt tgttttatgt 12900tgaagattat cgggagggtc ggttactcga aaattttcaa ttgcttcttt atgatttcaa 12960ttgaagcgag aaacctcgcc cggcgtcttg gaacgcaaca tggaccgaga accgcgcatc 13020catgactaag caaccggatc gacctattca ggccgcagtt ggtcaggtca ggctcagaac 13080gaaaatgctc ggcgaggtta cgctgtctgt aaacccattc gatgaacggg aagcttcctt 13140ccgattgctc ttggcaggaa tattggccca tgcctgcttg cgctttgcaa atgctcttat 13200cgcgttggta tcatatgcct tgtccgccag cagaaacgca ctctaagcga ttatttgtaa 13260aaatgtttcg gtcatgcggc ggtcatgggc ttgacccgct gtcagcgcaa gacggatcgg 13320tcaaccgtcg gcatcgacaa cagcgtgaat cttggtggtc aaaccgccac gggaacgtcc 13380catacagcca tcgtcttgat cccgctgttt cccgtcgccg catgttggtg gacgcggaca 13440caggaactgt caatcatgac gacattctat cgaaagcctt ggaaatcaca ctcagaatat 13500gatcccagac gtctgcctca cgccatcgta caaagcgatt gtagcaggtt gtacaggaac 13560cgtatcgatc aggaacgtct gcccagggcg ggcccgtccg gaagcgccac aagatgacat 13620tgatcacccg cgtcaacgcg cggcacgcga cgcggcttat ttgggaacaa aggactgaac 13680aacagtccat tcgaaatcgg tgacatcaaa gcggggacgg gttatcagtg gcctccaagt 13740caagcctcaa tgaatcaaaa tcagaccgat ttgcaaacct gatttatgag tgtgcggcct 13800aaatgatgaa atcgtccttc tagatcgcct ccgtggtgta gcaacacctc gcagtatcgc 13860cgtgctgacc ttggccaggg aattgactgg caagggtgct ttcacatgac cgctcttttg 13920gccgcgatag atgatttcgt tgctgctttg ggcacgtaga aggagagaag tcatatcgga 13980gaaattcctc ctggcgcgag agcctgctct atcgcgacgg catcccactg tcgggaacag 14040accggatcat tcacgaggcg aaagtcgtca acacatgcgt tataggcatc ttcccttgaa 14100ggatgatctt gttgctgcca atctggaggt gcggcagccg caggcagatg cgatctcagc 14160gcaacttgcg gcaaaacatc tcactcacct gaaaaccact agcgagtctc gcgatcagac 14220gaaggccttt tacttaacga cacaatatcc gatgtctgca tcacaggcgt cgctatccca 14280gtcaatacta aagcggtgca ggaactaaag attactgatg acttaggcgt gccacgaggc 14340ctgagacgac gcgcgtagac agttttttga aatcattatc aaagtgatgg cctccgctga 14400agcctatcac ctctgcgccg gtctgtcgga gagatgggca agcattatta cggtcttcgc 14460gcccgtacat gcattggacg attgcagggt caatggatct gagatcatcc agaggattgc 14520cgcccttacc ttccgtttcg agttggagcc agcccctaaa tgagacgaca tagtcgactt 14580gatgtgacaa tgccaagaga gagatttgct taacccgatt tttttgctca agcgtaagcc 14640tattgaagct tgccggcatg acgtccgcgc cgaaagaata tcctacaagt aaaacattct 14700gcacaccgaa atgcttggtg tagacatcga ttatgtgacc aagatcctta gcagtttcgc 14760ttggggaccg ctccgaccag aaataccgaa gtgaactgac gccaatgaca ggaatccctt 14820ccgtctgcag ataggtacca tcgatagatc tgctgcctcg cgcgtttcgg tgatgacggt 14880gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 14940gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 15000atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 15060agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 15120aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 15180ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 15240gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 15300aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 15360gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 15420ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 15480cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 15540cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 15600gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 15660cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 15720agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 15780ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 15840ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 15900gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 15960cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 16020attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 16080accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 16140ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 16200gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 16260agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 16320ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 16380ttgttgccat tgctgcaggg gggggggggg ggggggactt ccattgttca ttccacggac 16440aaaaacagag aaaggaaacg acagaggcca aaaagcctcg ctttcagcac ctgtcgtttc 16500ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa 16560cgccttaaac cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac 16620ctgtcggatc accggaaagg acccgtaaag tgataatgat tatcatctac atatcacaac 16680gtgcgtggag gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat 16740tgatctgcat caacttaacg taaaaacaac ttcagacaat acaaatcagc gacactgaat 16800acggggcaac ctcatgtccc cccccccccc ccccctgcag ggcatcgtgg tgtcacgctc 16860gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 16920ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 16980gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 17040gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 17100gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 17160tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 17220gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 17280agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 17340aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 17400ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 17460gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 17520agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 17580tcttcaagaa ttggtcgacg atcttgctgc gttcggatat tttcgtggag ttcccgccac 17640agacccggat tgaaggcgag atccagcaac tcgcgccaga tcatcctgtg acggaacttt 17700ggcgcgtgat gactggccag gacgtcggcc gaaagagcga caagcagatc acgcttttcg 17760acagcgtcgg atttgcgatc gaggattttt cggcgctgcg ctacgtccgc gaccgcgttg 17820agggatcaag ccacagcagc ccactcgacc ttctagccga cccagacgag ccaagggatc 17880tttttggaat gctgctccgt cgtcaggctt tccgacgttt gggtggttga acagaagtca 17940ttatcgtacg gaatgccaag cactcccgag gggaaccctg tggttggcat gcacatacaa 18000atggacgaac ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct 18060cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag 18120gcgggaaacg acaatctgat catgagcgga gaattaaggg agtcacgtta tgacccccgc 18180cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag 18240ccactcagcc caagcttgat atcgaattcc tgcagcccgg gggatccggg gcggaagatg 18300gcagggacgc ggattcaggg cggacgcgct tgccgagggc gcgggggacc acagcgtgcg 18360ttacggggac agggcgggca tcgcgaggac gggtgcggga gcggagccac atctggtggt 18420ggacgcctac tttgctctct tatagagtag taaagattcg tggaccaaac aacaccctag 18480cttgtacaaa tattcttagg cagttgctac tgatgagaga aaaataacat cactccactg 18540catttgcgtg atttattgaa cagatcacaa ttacatctat tcaaatttat ttacctgtac 18600gtgtccgatt tttaggggag gattttttta cggtattttt tttttaaaaa aataaattta 18660ggcaacaatt ttatagaatc gagtgcttta tctattatct tttacaaggc acacgcgtac 18720aataaggttt ggtcgttcgt gacttggata gtggttttgg ttgcaattcc gtaattcttg 18780gcataggata cagcccaacc cagaaaaaaa taatgttgcg gtcagttctg gctttgagat 18840tcggagtacc acgtggcgta aaggcaggcc gtgtcttaca gatgaataaa ggacctgggt 18900ctcacgtgat tggtttccag tttcgtgcat caagatgtgg aattttcaaa ctgccgtcgt 18960gtttgtttcg tcacataaaa gctttttgga aggctaagga gaggaagccg gcgagaagga 19020gggggcgttt tacgtgtcac tgtcctgtcg tgttggctgt tgacacgaat catttcttcc 19080gcgcgtggga agaagaagat gcacattagc ggcctgaagt agagatgtca atggggaatt 19140ccccagcggg gattaactcc ccagacccgt acccatgaac atagaccggc ccccatcccc 19200gaacccgaac ccgacctcgg gtacgaaaat cctcccatac ccattcccga ccgggtacta 19260aatacccatg ggtatccata cccgacccga ttattcaaaa attaatgggc tttttatttg 19320ttaaccggcg gacgcaatgc ttgggactct aggttttttt actttgttga ccggctggcg 19380gctgggcttt ttcctacagg cccaaagttg gtcggcagcc actaggccac acgtcacagg 19440cagcccacaa gtaaatgtcg ttggattgct ggatggtgga ataaaaatcc tagatgctag 19500attgttctgg ttccgggtat ttttctccat ggctaatcgg gtttgggttt agccctccca 19560aacccgaacc cgccataccc gatgggtaag ggatttattc caaatctata cccatgggga 19620tttgttttaa cccatacctt aaccctaata gaggaattcc ccacgggtaa tcgggtttcg 19680gggcccattg acatctctag actgaaggcg tccaactcaa atcattaaaa agtgttgacg 19740cacgcgctga tgcgccggcc gcacagcaca ggctgcacag cccgtttaat cagcgatgga 19800gccccggccg tcagccagcc aggtccggcg tccgggtctg cgccctgcgg cgtcactgct 19860gtcgccaccg tctccgatgg tcccacatcc atccagcggg ccgcgcgtgg tacaaaaggc 19920tcttcctcgc cgtcaggtgc agctgcccaa acaccagaca cagactccac caccccgctt 19980cgatcttctg ttgcagctga aatctgtcag attctgcagt tcattcctca tggagaagag 20040gaacctgcag tggcggcgag ggcgtgatgg catcgtgcag taccctcacc tcttcttcgc 20100ggccctggcg ctggccctcc tagtcgcgga ccgttcggcc tcagtccgct ggccgaggtc 20160gactaccggc cggtgaagca cgagctcgcg ccgtacgggg aggtcatggg cagctggccc 20220agagacaatg ccagccggct caggcgcggg aggctggagt tcgtcggcga ggtgttcggg 20280ccggagtcta tcgagttcga tctccagggc cgcgggccgt acgccggcct cgccgacggc 20340cgcgtcgtgc ggtggatggg cgaggaggcc gggtgggaga cgttcgccgg tcatgaatcc 20400tgactggtaa gtgctcgata tgcctccggc gtccactcgt tacagtgcta taatatagta 20460gtactaagat attttgatct gattttttgc attcttggga gaaacgtcat gcaaaatttg 20520ttgtttcttg gcaaaggtca gaagaagtct gtgccaatgg agtgaactca acgacgagga 20580agcagcacga gaaggaggag ttctgcggcg gccgctcggc ctgaggttcc acggggagac 20640cggcgagctc tacgtcgccg acgcgtacta cggtctcatg gtcgttggcc agagcggcgg 20700cgtggcgtcc tccgtcgcga gggaagccga cggggacccc atccggttcg cgaacgacct 20760cgatgtgcac aggaatggat ccgtattctt cactgacacg agcatgagat acagcagaaa 20820gtgagcaaag cagcgtaaca atccggcttc tcattttcaa acgcctctgt attctctgct 20880gaaagagtag ctcaccagac aagagctgaa tttgcaggga ccatctgaac atcctgttag 20940aaggagaagg caccgggagg ctgctcaggt atgatccaga aacaagcggt gtccatgtcg 21000tgctcaaggg gctggtgttc ccaaacggcg tgcagatctc agaggaccat cagtttcttc 21060tcttctccga gacaacaaac

tgcaggtaac aaaaatacta tctgacgatg ctcatgattc 21120taccgtatcc atagtcatga acacaaacca cacgaatctg gccttgacca ggataatgag 21180gtactggctg gaaggcccaa gagcgggcga ggtagaggtg ttcgcgaacc tgccgggctt 21240ccccgacaac gtgcgctcca acggcagggg ccagttctgg gtggcgatcg actgctgccg 21300gaccgaggag gtgttcccaa gagcgtggct ccggaccctg tacttcaagt tcccgctgtc 21360gctcaaggtg ctcacttgga aggccgccag gaggatgcac acggtgctcg cgctcctcga 21420cggcgaaggg cgcgtcgtgg aggtgctcga ggaccggggc cacgaggtga tgaagctggt 21480gagcgaggtg cgggaggtgg gccgcaagct gtggatcgga accgtggcgc acaaccacat 21540cgccaccatc ccctaccctt tagaggacta accatgatct atgctgtttc aatgcctcct 21600aatctgtgta cgtctataaa tgtctaatgc agctcatggt tgtaatcttg tttgtgtttg 21660gcaaattggc ataataatgg acagattcaa tgggcattgg tgctgtagtc gcatcacact 21720aattgaatgg gatcatgttg agctctcact ttgctacaat ttgctccagc ttgtacggtt 21780gtaccctctt gctcgtctat agtaagggcc atctaaaaaa aactcaaatt agatctgcaa 21840tacaagtatg attgggccga atttggattg tcacgggtcc gcgaccgcga attgggctcc 21900ggtttgattt agccgacata gtagtgaccg acccgagccg gccggcgaga ccaaaccgag 21960cggacgcccg ccatgcatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt 22020aaagactggc gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt 22080cgtcaacatg gtggagcacg acacgcttgt ctactccaaa aatatcaaag atacagtctc 22140agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg 22200attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc 22260ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag 22320tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac 22380cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca 22440atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag 22500gacagggtac ccggggatcc accatgtctc cggagaggag accagttgag attaggccag 22560ctacagcagc tgatatggcc gcggtttgtg atatcgttaa ccattacatt gagacgtcta 22620cagtgaactt taggacagag ccacaaacac cacaagagtg gattgatgat ctagagaggt 22680tgcaagatag atacccttgg ttggttgctg aggttgaggg tgttgtggct ggtattgctt 22740acgctgggcc ctggaaggct aggaacgctt acgattggac agttgagagt actgtttacg 22800tgtcacatag gcatcaaagg ttgggcctag gatccacatt gtacacacat ttgcttaagt 22860ctatggaggc gcaaggtttt aagtctgtgg ttgctgttat aggccttcca aacgatccat 22920ctgttaggtt gcatgaggct ttgggataca cagcccgggg tacattgcgc gcagctggat 22980acaagcatgg tggatggcat gatgttggtt tttggcaaag ggattttgag ttgccagctc 23040ctccaaggcc agttaggcca gttacccaga tctgagtcga cctgcaggca tgccgctgaa 23100atcaccagtc tctctctaca aatctatctc tctctataat aatgtgtgag tagttcccag 23160ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt 23220agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa 23280aatccagtgg gtaccgagct cgaattcagt acattaaaaa cgtccgcaat gtgttattaa 23340gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc 23400tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg 23460acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat 23520tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc 23580atgttgattg taacgatgac agagcgttgc tgcctgtgat caaatatcat ctccctcgca 23640gagatccgaa ttatcagcct tcttattcat ttctcgctta accgtgacag gctgtcgatc 23700ttgagaacta tgccgacata ataggaaatc gctggataaa gccgctgagg aagctgagtg 23760gcgctatttc tttagaagtg aacgttgacg atcgtcgacc gtaccccgat gaattaattc 23820ggacgtacgt tctgaacaca gctggatact tacttgggcg attgtcatac atgacatcaa 23880caatgtaccc gtttgtgtaa ccgtctcttg gaggttcgta tgacactagt ggttcccctc 23940agcttgcgac tagatgttga ggcctaacat tttattagag agcaggctag ttgcttagat 24000acatgatctt caggccgtta tctgtcaggg caagcgaaaa ttggccattt atgacgacca 24060atgccccgca gaagctccca tctttgccgc catagacgcc gcgcccccct tttggggtgt 24120agaacatcct tttgccagat gtggaaaaga agttcgttgt cccattgttg gcaatgacgt 24180agtagccggc gaaagtgcga gacccatttg cgctatatat aagcctacga tttccgttgc 24240gactattgtc gtaattggat gaactattat cgtagttgct ctcagagttg tcgtaatttg 24300atggactatt gtcgtaattg cttatggagt tgtcgtagtt gcttggagaa atgtcgtagt 24360tggatgggga gtagtcatag ggaagacgag cttcatccac taaaacaatt ggcaggtcag 24420caagtgcctg ccccgatgcc atcgcaagta cgaggcttag aaccaccttc aacagatcgc 24480gcatagtctt ccccagctct ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc 24540ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt 24600gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttgt ccaagataag 24660cctgcctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt 24720cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca 24780acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta 24840aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg 24900ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat 24960caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc 25020caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa 25080tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa 25140ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca 25200aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg 25260ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg 25320atacttcggc gatcaccgct tccctcatga tgtttaactc ctgaattaag ccgcgccgcg 25380aagcggtgtc ggcttgaatg aattgttagg cgtcatcctg tgctcccgag aaccagtacc 25440agtacatcgc tgtttcgttc gagacttgag gtctagtttt atacgtgaac aggtcaatgc 25500cgccgagagt aaagccacat tttgcgtaca aattgcaggc aggtacattg ttcgtttgtg 25560tctctaatcg tatgccaagg agctgtctgc ttagtgccca ctttttcgca aattcgatga 25620gactgtgcgc gactcctttg cctcggtgcg tgtgcgacac aacaatgtgt tcgatagagg 25680ctagatcgtt ccatgttgag ttgagttcaa tcttcccgac aagctcttgg tcgatgaatg 25740cgccatagca agcagagtct tcatcagagt catcatccga gatgtaatcc ttccggtagg 25800ggctcacact tctggtagat agttcaaagc cttggtcgga taggtgcaca tcgaacactt 25860cacgaacaat gaaatggttc tcagcatcca atgtttccgc cacctgctca gggatcaccg 25920aaatcttcat atgacgccta acgcctggca cagcggatcg caaacctggc gcggcttttg 25980gcacaaaagg cgtgacaggt ttgcgaatcc gttgctgcca cttgttaacc cttttgccag 26040atttggtaac tataatttat gttagaggcg aagtcttggg taaaaactgg cctaaaattg 26100ctggggattt caggaaagta aacatcacct tccggctcga tgtctattgt agatatatgt 26160agtgtatcta cttgatcggg ggatctgctg cctcgcgcgt ttcggtgatg acggtgaaaa 26220cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 26280cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac 26340ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt 26400gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 26460cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 26520cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 26580aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 26640gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 26700tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 26760agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 26820ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 26880taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 26940gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 27000gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 27060ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 27120ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 27180gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 27240caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 27300taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 27360aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 27420tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 27480tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 27540gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 27600gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 27660aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 27720gccattgctg cagggggggg gggggggggg gacttccatt gttcattcca cggacaaaaa 27780cagagaaagg aaacgacaga ggccaaaaag cctcgctttc agcacctgtc gtttcctttc 27840ttttcagagg gtattttaaa taaaaacatt aagttatgac gaagaagaac ggaaacgcct 27900taaaccggaa aattttcata aatagcgaaa acccgcgagg tcgccgcccc gtaacctgtc 27960ggatcaccgg aaaggacccg taaagtgata atgattatca tctacatatc acaacgtgcg 28020tggaggccat caaaccacgt caaataatca attatgacgc aggtatcgta ttaattgatc 28080tgcatcaact taacgtaaaa acaacttcag acaatacaaa tcagcgacac tgaatacggg 28140gcaacctcat gtcccccccc cccccccccc tgcaggcatc gtggtgtcac gctcgtcgtt 28200tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 28260gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 28320cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 28380cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 28440gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag 28500aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 28560accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 28620ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 28680gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 28740aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 28800taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 28860cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca 28920agaattcgga gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac 28980ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg 29040gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc 29100cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat 29160tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac 29220actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt 29280tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa 29340gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc 29400cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc 29460ttcacgaggc agacctcagc gccagaaggc cgccagagag gccgagcgcg gccgtgaggc 29520ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc tgctacgggc gtctgacgcg 29580gtggaaaggg ggaggggatg ttgtctacat ggctctgctg tagtgagtgg gttgcgctcc 29640ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt caacgttcct gacaacgagc 29700ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc tgcgtccgga 29760ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg agcctgttca 29820acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag cacggcccca 29880acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga aaaacccgcc 29940tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc cggtcgcgtg 30000ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa gctgcgggca 30060ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg cgtatgattc 30120tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct gaagtgccag 30180taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt cagaccgtct 30240acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg ctgcaacttt 30300gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta tcagtgataa 30360agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac gtgaaaccca 30420acatacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc atcggcctga 30480ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac gtcaccgccc 30540actatggcat tctgctggcg ctgtatgcgt tggtgcaatt tgcctgcgca cctgtgctgg 30600gcgcgctgtc ggatcgtttc gggcggcggc caatcttgct cgtctcgctg gccggcgcca 30660ctgtcgacta cgccatcatg gcgacagcgc ctttcctttg ggttctctat atcgggcgga 30720tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt gccgatatca 30780ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc gggttcggga 30840tggtcgcggg acctgtgctc ggtgggctga tgggcggttt ctccccccac gctccgttct 30900tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt ttgccggagt 30960cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc gcttcgttcc 31020ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc atcatgcaac 31080ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc tttcactggg 31140acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca ctcgcccagg 31200caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc atgctcggaa 31260tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga tggatggcgt 31320tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg caagcaatgt 31380tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg gcggcgctca 31440ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg gcttctataa 31500caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc tgcctgccgg 31560cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga tcgtggaaac 31620gataggccta tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg ccctaggagt 31680gcggttggaa cgttggccca gccagatact cccgatcacg agcaggacgc cgatgatttg 31740aagcgcactc agcgtctgat ccaagaacaa ccatcctagc aacacggcgg tccccgggct 31800gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc ggaaccaaag 31860gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa cattggttcc 31920tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca gaagtcctcc 31980ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc acttgcgggt 32040cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga cgccgacagg 32100atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc 32160ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga tgaacacgac 32220catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga 32280aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga agatgcgcat 32340ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac ccgccggcaa 32400cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc 32460ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc 32520aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa 32580cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct ctggagcttt 32640cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc caagccgtcg 32700aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca gttcgtagag 32760cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt 32820tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg 32880ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg 32940cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa 33000tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag 33060ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg 33120aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg 33180caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat 33240tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg 33300cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc 33360tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc 33420tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc 33480acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg 33540ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc 33600ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt 33660ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt 33720tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct 33780gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca 33840gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag 33900ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg 33960gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac 34020gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct 34080tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg 34140aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc 34200tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca 34260aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac 34320tcttcattaa ccgctatatc gaaaattgct tgcggcttgt tagaattgcc atgacgtacc 34380tcggtgtcac gggtaagatt accgataaac tggaactgat tatggctcat atcgaaagtc 34440tccttgagaa aggagactct agtttagcta aacattggtt ccgctgtcaa gaactttagc 34500ggctaaaatt ttgcgggccg cgaccaaagg tgcgaggggc ggcttccgct gtgtacaacc 34560agatattttt caccaacatc cttcgtctgc tcgatgagcg gggcatgacg aaacatgagc 34620tgtcggagag ggcaggggtt tcaatttcgt ttttatcaga cttaaccaac ggtaaggcca 34680acccctcgtt gaaggtgatg gaggccattg ccgacgccct ggaaactccc ctacctcttc 34740tcctggagtc caccgacctt gaccgcgagg cactcgcgga gattgcgggt catcctttca 34800agagcagcgt gccgcccgga tacgaacgca tcagtgtggt tttgccgtca cataaggcgt 34860ttatcgtaaa gaaatggggc gacgacaccc gaaaaaagct gcgtggaagg ctctgacgcc 34920aagggttagg gcttgcactt ccttctttag ccgctaaaac ggccccttct ctgcgggccg 34980tcggctcgcg catcatatcg acatcctcaa cggaagccgt gccgcgaatg gcatcgggcg 35040ggtgcgcttt gacagttgtt ttctatcaga acccctacgt cgtgcggttc gattagctgt 35100ttgtcttgca ggctaaacac tttcggtata tcgtttgcct gtgcgataat gttgctaatg 35160atttgttgcg taggggttac tgaaaagtga gcgggaaaga agagtttcag accatcaagg 35220agcgggccaa gcgcaagctg gaacgcgaca tgggtgcgga cctgttggcc gcgctcaacg 35280acccgaaaac cgttgaagtc atgctcaacg cggacggcaa ggtgtggcac gaacgccttg 35340gcgagccgat gcggtacatc tgcgacatgc ggcccagcca gtcgcaggcg attatagaaa 35400cggtggccgg attccacggc aaagaggtca cgcggcattc gcccatcctg gaaggcgagt 35460tccccttgga tggcagccgc tttgccggcc aattgccgcc ggtcgtggcc gcgccaacct 35520ttgcgatccg caagcgcgcg gtcgccatct tcacgctgga acagtacgtc gaggcgggca 35580tcatgacccg cgagcaatac gaggtcatta aaagcgccgt cgcggcgcat cgaaacatcc 35640tcgtcattgg cggtactggc tcgggcaaga ccacgctcgt caacgcgatc atcaatgaaa 35700tggtcgcctt caacccgtct gagcgcgtcg tcatcatcga ggacaccggc gaaatccagt 35760gcgccgcaga gaacgccgtc caataccaca ccagcatcga cgtctcgatg acgctgctgc 35820tcaagacaac gctgcgtatg cgccccgacc gcatcctggt cggtgaggta cgtggccccg 35880aagcccttga tctgttgatg gcctggaaca ccgggcatga aggaggtgcc gccaccctgc 35940acgcaaacaa ccccaaagcg ggcctgagcc ggctcgccat gcttatcagc atgcacccgg 36000attcaccgaa acccattgag ccgctgattg gcgaggcggt tcatgtggtc gtccatatcg 36060ccaggacccc tagcggccgt cgagtgcaag aaattctcga agttcttggt tacgagaacg 36120gccagtacat caccaaaacc

ctgtaaggag tatttccaat gacaacggct gttccgttcc 36180gtctgaccat gaatcgcggc attttgttct accttgccgt gttcttcgtt ctcgctctcg 36240cgttatccgc gcatccggcg atggcctcgg aaggcaccgg cggcagcttg ccatatgaga 36300gctggctgac gaacctgcgc aactccgtaa ccggcccggt ggccttcgcg ctgtccatca 36360tcggcatcgt cgtcgccggc ggcgtgctga tcttcggcgg cgaactcaac gccttcttcc 36420gaaccctgat cttcctggtt ctggtgatgg cgctgctggt cggcgcgcag aacgtgatga 36480gcaccttctt cggtcgtggt gccgaaatcg cggccctcgg caacggggcg ctgcaccagg 36540tgcaagtcgc ggcggcggat gccgtgcgtg cggtagcggc tggacggctc gcctaatcat 36600ggctctgcgc acgatcccca tccgtcgcgc aggcaaccga gaaaacctgt tcatgggtgg 36660tgatcgtgaa ctggtgatgt tctcgggcct gatggcgttt gcgctgattt tcagcgccca 36720agagctgcgg gccaccgtgg tcggtctgat cctgtggttc ggggcgctct atgcgttccg 36780aatcatggcg aaggccgatc cgaagatgcg gttcgtgtac ctgcgtcacc gccggtacaa 36840gccgtattac ccggcccgct cgaccccgtt ccgcgagaac accaatagcc aagggaagca 36900ataccgatga tccaagcaat tgcgattgca atcgcgggcc tcggcgcgct tctgttgttc 36960atcctctttg cccgcatccg cgcggtcgat gccgaactga aactgaaaaa gcatcgttcc 37020aaggacgccg gcctggccga tctgctcaac tacgccgctg tcgtcgatga cggcgtaatc 37080gtgggcaaga acggcagctt tatggctgcc tggctgtaca agggcgatga caacgcaagc 37140agcaccgacc agcagcgcga agtagtgtcc gcccgcatca accaggccct cgcgggcctg 37200ggaagtgggt ggatgatcca tgtggacgcc gtgcggcgtc ctgctccgaa ctacgcggag 37260cggggcctgt cggcgttccc tgaccgtctg acggcagcga ttgaagaaga gcgctcggtc 37320ttgccttgct cgtcggtgat gtacttcacc agctccgcga agtcgctctt cttgatggag 37380cgcatgggga cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag 37440tcatggctct gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt 37500ctcttcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg 37560gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg 37620ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac 37680ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct 37740tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt 37800ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag 37860agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc 37920gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg 37980aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac 38040cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagc gctgcttccc 38100tgctgttttg tggaatatct accgactgga aacaggcaaa tgcaggaaat tactgaactg 38160aggggacagg cgagagacga tgccaaagag ctacaccgac gagctggccg agtgggttga 38220atcccgcgcg gccaagaagc gccggcgtga tgaggctgcg gttgcgttcc tggcggtgag 38280ggcggatgtc gaggcggcgt tagcgtccgg ctatgcgctc gtcaccattt gggagcacat 38340gcgggaaacg gggaaggtca agttctccta cgagacgttc cgctcgcacg ccaggcggca 38400catcaaggcc aagcccgccg atgtgcccgc accgcaggcc aaggctgcgg aacccgcgcc 38460ggcacccaag acgccggagc cacggcggcc gaagcagggg ggcaaggctg aaaagccggc 38520ccccgctgcg gccccgaccg gcttcacctt caacccaaca ccggacaaaa aggatctact 38580gtaatggcga aaattcacat ggttttgcag ggcaagggcg gggtcggcaa gtcggccatc 38640gccgcgatca ttgcgcagta caagatggac aaggggcaga cacccttgtg catcgacacc 38700gacccggtga acgcgacgtt cgagggctac aaggccctga acgtccgccg gctgaacatc 38760atggccggcg acgaaattaa ctcgcgcaac ttcgacaccc tggtcgagct gattgcgccg 38820accaaggatg acgtggtgat cgacaacggt gccagctcgt tcgtgcctct gtcgcattac 38880ctcatcagca accaggtgcc ggctctgctg caagaaatgg ggcatgagct ggtcatccat 38940accgtcgtca ccggcggcca ggctctcctg gacacggtga gcggcttcgc ccagctcgcc 39000agccagttcc cggccgaagc gcttttcgtg gtctggctga acccgtattg ggggcctatc 39060gagcatgagg gcaagagctt tgagcagatg aaggcgtaca cggccaacaa ggcccgcgtg 39120tcgtccatca tccagattcc ggccctcaag gaagaaacct acggccgcga tttcagcgac 39180atgctgcaag agcggctgac gttcgaccag gcgctggccg atgaatcgct cacgatcatg 39240acgcggcaac gcctcaagat cgtgcggcgc ggcctgtttg aacagctcga cgcggcggcc 39300gtgctatgag cgaccagatt gaagagctga tccgggagat tgcggccaag cacggcatcg 39360ccgtcggccg cgacgacccg gtgctgatcc tgcataccat caacgcccgg ctcatggccg 39420acagtgcggc caagcaagag gaaatccttg ccgcgttcaa ggaagagctg gaagggatcg 39480cccatcgttg gggcgaggac gccaaggcca aagcggagcg gatgctgaac gcggccctgg 39540cggccagcaa ggacgcaatg gcgaaggtaa tgaaggacag cgccgcgcag gcggccgaag 39600cgatccgcag ggaaatcgac gacggccttg gccgccagct cgcggccaag gtcgcggacg 39660cgcggcgcgt ggcgatgatg aacatgatcg ccggcggcat ggtgttgttc gcggccgccc 39720tggtggtgtg ggcctcgtta tgaatcgcag aggcgcagat gaaaaagccc ggcgttgccg 39780ggctttgttt ttgcgttagc tgggcttgtt tgacaggccc aagctctgac tgcgcccgcg 39840ctcgcgctcc tgggcctgtt tcttctcctg ctcctgcttg cgcatcaggg cctggtgccg 39900tcgggctgct tcacgcatcg aatcccagtc gccggccagc tcgggatgct ccgcgcgcat 39960cttgcgcgtc gccagttcct cgatcttggg cgcgtgaatg cccatgcctt ccttgatttc 40020gcgcaccatg tccagccgcg tgtgcagggt ctgcaagcgg gcttgctgtt gggcctgctg 40080ctgctgccag gcggcctttg tacgcggcag ggacagcaag ccgggggcat tggactgtag 40140ctgctgcaaa cgcgcctgct gacggtctac gagctgttct aggcggtcct cgatgcgctc 40200cacctggtca tgctttgcct gcacgtagag cgcaagggtc tgctggtagg tctgctcgat 40260gggcgcggat tctaagaggg cctgctgttc cgtctcggcc tcctgggccg cctgtagcaa 40320atcctcgccg ctgttgccgc tggactgctt tactgccggg gactgctgtt gccctgctcg 40380cgccgtcgtc gcagttcggc ttgcccccac tcgattgact gcttcatttc gagccgcagc 40440gatgcgatct cggattgcgt caacggacgg ggcagcgcgg aggtgtccgg cttctccttg 40500ggtgagtcgg tcgatgccat agccaaaggt ttccttccaa aatgcgtcca ttgctggacc 40560gtgtttctca ttgatgcccg caagcatctt cggcttgacc gccaggtcaa gcgcgccttc 40620atgggcggtc atgacggacg ccgccatgac cttgccgccg ttgttctcga tgtagccgcg 40680taatgaggca atggtgccgc ccatcgtcag cgtgtcatcg acaacgatgt acttctggcc 40740ggggatcacc tccccctcga aagtcgggtt gaacgccagg cgatgatctg aaccggctcc 40800ggttcgggcg accttctccc gctgcacaat gtccgtttcg acctcaaggc caaggcggtc 40860ggccagaacg accgccatca tggccggaat cttgttgttc cccgccgcct cgacggcgag 40920gactggaacg atgcggggct tgtcgtcgcc gatcagcgtc ttgagctggg caacagtgtc 40980gtccgaaatc aggcgctcga ccaaattaag cgccgcttcc gcgtcgccct gcttcgcagc 41040ctggtattca ggctcgttgg tcaaagaacc aaggtcgccg ttgcgaacca ccttcgggaa 41100gtctccccac ggtgcgcgct cggctctgct gtagctgctc aagacgcctc cctttttagc 41160cgctaaaact ctaacgagtg cgcccgcgac tcaacttgac gctttcggca cttacctgtg 41220ccttgccact tgcgtcatag gtgatgcttt tcgcactccc gatttcaggt actttatcga 41280aatctgaccg ggcgtgcatt acaaagttct tccccacctg ttggtaaatg ctgccgctat 41340ctgcgtggac gatgctgccg tcgtggcgct gcgacttatc ggccttttgg gccatataga 41400tgttgtaaat gccaggtttc agggccccgg ctttatctac cttctggttc gtccatgcgc 41460cttggttctc ggtctggaca attctttgcc cattcatgac caggaggcgg tgtttcattg 41520ggtgactcct gacggttgcc tctggtgtta aacgtgtcct ggtcgcttgc cggctaaaaa 41580aaagccgacc tcggcagttc gaggccggct ttccctagag ccgggcgcgt caaggttgtt 41640ccatctattt tagtgaactg cgttcgattt atcagttact ttcctcccgc tttgtgtttc 41700ctcccactcg tttccgcgtc tagccgaccc ctcaacatag cggcctcttc ttgggctgcc 41760tttgcctctt gccgcgcttc gtcacgctcg gcttgcaccg tcgtaaagcg ctcggcctgc 41820ctggccgcct cttgcgccgc caacttcctt tgctcctggt gggcctcggc gtcggcctgc 41880gccttcgctt tcaccgctgc caactccgtg cgcaaactct ccgcttcgcg cctggtggcg 41940tcgcgctcgc cgcgaagcgc ctgcatttcc tggttggccg cgtccagggt cttgcggctc 42000tcttctttga atgcgcgggc gtcctggtga gcgtagtcca gctcggcgcg cagctcctgc 42060gctcgacgct ccacctcgtc ggcccgctgc gtcgccagcg cggcccgctg ctcggctcct 42120gccagggcgg tgcgtgcttc ggccagggct tgccgctggc gtgcggccag ctcggccgcc 42180tcggcggcct gctgctctag caatgtaacg cgcgcctggg cttcttccag ctcgcgggcc 42240tgcgcctcga aggcgtcggc cagctccccg cgcacggctt ccaactcgtt gcgctcacga 42300tcccagccgg cttgcgctgc ctgcaacgat tcattggcaa gggcctgggc ggcttgccag 42360agggcggcca cggcctggtt gccggcctgc tgcaccgcgt ccggcacctg gactgccagc 42420ggggcggcct gcgccgtgcg ctggcgtcgc cattcgcgca tgccggcgct ggcgtcgttc 42480atgttgacgc gggcggcctt acgcactgca tccacggtcg ggaagttctc ccggtcgcct 42540tgctcgaaca gctcgtccgc agccgcaaaa atgcggtcgc gcgtctcttt gttcagttcc 42600atgttggctc cggtaattgg taagaataat aatactctta cctaccttat cagcgcaaga 42660gtttagctga acagttctcg acttaacggc aggtttttta gcggctgaag ggcaggcaaa 42720aaaagccccg cacggtcggc gggggcaaag ggtcagcggg aaggggatta gcgggcgtcg 42780ggcttcttca tgcgtcgggg ccgcgcttct tgggatggag cacgacgaag cgcgcacgcg 42840catcgtcctc ggccctatcg gcccgcgtcg cggtcaggaa cttgtcgcgc gctaggtcct 42900ccctggtggg caccaggggc atgaactcgg cctgctcgat gtaggtccac tccatgaccg 42960catcgcagtc gaggccgcgt tccttcaccg tctcttgcag gtcgcggtac gcccgctcgt 43020tgagcggctg gtaacgggcc aattggtcgt aaatggctgt cggccatgag cggcctttcc 43080tgttgagcca gcagccgacg acgaagccgg caatgcaggc ccctggcaca accaggccga 43140cgccgggggc aggggatggc agcagctcgc caaccaggaa ccccgccgcg atgatgccga 43200tgccggtcaa ccagcccttg aaactatccg gccccgaaac acccctgcgc attgcctgga 43260tgctgcgccg gatagcttgc aacatcagga gccgtttctt ttgttcgtca gtcatggtcc 43320gccctcacca gttgttcgta tcggtgtcgg acgaactgaa atcgcaagag ctgccggtat 43380cggtccagcc gctgtccgtg tcgctgctgc cgaagcacgg cgaggggtcc gcgaacgccg 43440cagacggcgt atccggccgc agcgcatcgc ccagcatggc cccggtcagc gagccgccgg 43500ccaggtagcc cagcatggtg ctgttggtcg ccccggccac cagggccgac gtgacgaaat 43560cgccgtcatt ccctctggat tgttcgctgc tcggcggggc agtgcgccgc gccggcggcg 43620tcgtggatgg ctcgggttgg ctggcctgcg acggccggcg aaaggtgcgc agcagctcgt 43680tatcgaccgg ctgcggcgtc ggggccgccg ccttgcgctg cggtcggtgt tccttcttcg 43740gctcgcgcag cttgaacagc atgatcgcgg aaaccagcag caacgccgcg cctacgcctc 43800ccgcgatgta gaacagcatc ggattcattc ttcggtcctc cttgtagcgg aaccgttgtc 43860tgtgcggcgc gggtggcccg cgccgctgtc tttggggatc agccctcgat gagcgcgacc 43920agtttcacgt cggcaaggtt cgcctcgaac tcctggccgt cgtcctcgta cttcaaccag 43980gcatagcctt ccgccggcgg ccgacggttg aggataaggc gggcagggcg ctcgtcgtgc 44040tcgacctgga cgatggcctt tttcagcttg tccgggtccg gctccttcgc gcccttttcc 44100ttggcgtcct taccgtcctg gtcgccgtcc tcgccgtcct ggccgtcgcc ggcctccgcg 44160tcacgctcgg catcagtctg gccgttgaag gcatcgacgg tgttgggatc gcggcccttc 44220tcgtccagga actcgcgcag cagcttgacc gtgccgcgcg tgatttcctg ggtgtcgtcg 44280tcaagccacg cctcgacttc ctccgggcgc ttcttgaagg ccgtcaccag ctcgttcacc 44340acggtcacgt cgcgcacgcg gccggtgttg aacgcatcgg cgatcttctc cggcaggtcc 44400agcagcgtga cgtgctgggt gatgaacgcc ggcgacttgc cgatttcctt ggcgatatcg 44460cctttcttct tgcccttcgc cagctcgcgg ccaatgaagt cggcaatttc gcgcggggtc 44520agctcgttgc gttgcaggtt ctcgataacc tggtcggctt cgttgtagtc gttgtcgatg 44580aacgccggga tggacttctt gccggcccac ttcgagccac ggtagcggcg ggcgccgtga 44640ttgatgatat agcggcccgg ctgctcctgg ttctcgcgca ccgaaatggg tgacttcacc 44700ccgcgctctt tgatcgtggc accgatttcc gcgatgctct ccggggaaaa gccggggttg 44760tcggccgtcc gcggctgatg cggatcttcg tcgatcaggt ccaggtccag ctcgataggg 44820ccggaaccgc cctgagacgc cgcaggagcg tccaggaggc tcgacaggtc gccgatgcta 44880tccaacccca ggccggacgg ctgcgccgcg cctgcggctt cctgagcggc cgcagcggtg 44940tttttcttgg tggtcttggc ttgagccgca gtcattggga aatctccatc ttcgtgaaca 45000cgtaatcagc cagggcgcga acctctttcg atgccttgcg cgcggccgtt ttcttgatct 45060tccagaccgg cacaccggat gcgagggcat cggcgatgct gctgcgcagg ccaacggtgg 45120ccggaatcat catcttgggg tacgcggcca gcagctcggc ttggtggcgc gcgtggcgcg 45180gattccgcgc atcgaccttg ctgggcacca tgccaaggaa ttgcagcttg gcgttcttct 45240ggcgcacgtt cgcaatggtc gtgaccatct tcttgatgcc ctggatgctg tacgcctcaa 45300gctcgatggg ggacagcaca tagtcggccg cgaagagggc ggccgccagg ccgacgccaa 45360gggtcggggc cgtgtcgatc aggcacacgt cgaagccttg gttcgccagg gccttgatgt 45420tcgccccgaa cagctcgcgg gcgtcgtcca gcgacagccg ttcggcgttc gccagtaccg 45480ggttggactc gatgagggcg aggcgcgcgg cctggccgtc gccggctgcg ggtgcggttt 45540cggtccagcc gccggcaggg acagcgccga acagcttgct tgcatgcagg ccggtagcaa 45600agtccttgag cgtgtaggac gcattgccct gggggtccag gtcgatcacg gcaacccgca 45660agccgcgctc gaaaaagtcg aaggcaagat gcacaagggt cgaagtcttg ccgacgccgc 45720ctttctggtt ggccgtgacc aaagttttca tcgtttggtt tcctgttttt tcttggcgtc 45780cgcttcccac ttccggacga tgtacgcctg atgttccggc agaaccgccg ttacccgcgc 45840gtacccctcg ggcaagttct tgtcctcgaa cgcggcccac acgcgatgca ccgcttgcga 45900cactgcgccc ctggtcagtc ccagcgacgt tgcgaacgtc gcctgtggct tcccatcgac 45960taagacgccc cgcgctatct cgatggtctg ctgccccact tccagcccct ggatcgcctc 46020ctggaactgg ctttcggtaa gccgtttctt catggataac acccataatt tgctccgcgc 46080cttggttgaa catagcggtg acagccgcca gcacatgaga gaagtttagc taaacatttc 46140tcgcacgtca acacctttag ccgctaaaac tcgtccttgg cgtaacaaaa caaaagcccg 46200gaaaccgggc tttcgtctct tgccgcttat ggctctgcac ccggctccat caccaacagg 46260tcgcgcacgc gcttcactcg gttgcggatc gacactgcca gcccaacaaa gccggttgcc 46320gccgccgcca ggatcgcgcc gatgatgccg gccacaccgg ccatcgccca ccaggtcgcc 46380gccttccggt tccattcctg ctggtactgc ttcgcaatgc tggacctcgg ctcaccatag 46440gctgaccgct cgatggcgta tgccgcttct ccccttggcg taaaacccag cgccgcaggc 46500ggcattgcca tgctgcccgc cgctttcccg accacgacgc gcgcaccagg cttgcggtcc 46560agaccttcgg ccacggcgag ctgcgcaagg acataatcag ccgccgactt ggctccacgc 46620gcctcgatca gctcttgcac tcgcgcgaaa tccttggcct ccacggccgc catgaatcgc 46680gcacgcggcg aaggctccgc agggccggcg tcgtgatcgc cgccgagaat gcccttcacc 46740aagttcgacg acacgaaaat catgctgacg gctatcacca tcatgcagac ggatcgcacg 46800aacccgctga attgaacacg agcacggcac ccgcgaccac tatgccaaga atgcccaagg 46860taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa gtgaagggca 46920ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat gtcgatgcca 46980gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc catcccgtta 47040ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt 47100tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg 47160gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc 47220tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg 47280tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc 47340tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat 47400cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc 47460cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt 47520tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac 47580gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg 47640gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc 47700gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca 47760accagcccgg tgagcgtcgg aaaggcgctg gaagccccgt agcgacgcgg agaggggcga 47820gacaagccaa gggcgcaggc tcgatgcgca gcacgacata gccggttctc gcaaggacga 47880gaatttccct gcggtgcccc tcaagtgtca atgaaagttt ccaacgcgag ccattcgcga 47940gagccttgag tccacgctag atgagagctt tgttgtaggt ggaccagttg gtgattttga 48000acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca 48060actcagcaaa agttcgattt attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt 48120acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca 48180gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg ac 48232151055DNAartificial sequenceZMCAS1HINDIIIPRO fragment comprising 1.0 KB ZmCAS1 promoter 15aagctttttg gaaggctaag gagaggaagc cggcgagaag gagggggcgt tttacgtgtc 60actgtcctgt cgtgttggct gttgacacga atcatttctt ccgcgcgtgg gaagaagaag 120atgcacatta gcggcctgaa gtagagatgt caatggggaa ttccccagcg gggattaact 180ccccagaccc gtacccatga acatagaccg gcccccatcc ccgaacccga acccgacctc 240gggtacgaaa atcctcccat acccattccc gaccgggtac taaataccca tgggtatcca 300tacccgaccc gattattcaa aaattaatgg gctttttatt tgttaaccgg cggacgcaat 360gcttgggact ctaggttttt ttactttgtt gaccggctgg cggctgggct ttttcctaca 420ggcccaaagt tggtcggcag ccactaggcc acacgtcaca ggcagcccac aagtaaatgt 480cgttggattg ctggatggtg gaataaaaat cctagatgct agattgttct ggttccgggt 540atttttctcc atggctaatc gggtttgggt ttagccctcc caaacccgaa cccgccatac 600ccgatgggta agggatttat tccaaatcta tacccatggg gatttgtttt aacccatacc 660ttaaccctaa tagaggaatt ccccacgggt aatcgggttt cggggcccat tgacatctct 720agactgaagg cgtccaactc aaatcattaa aaagtgttga cgcacgcgct gatgcgccgg 780ccgcacagca caggctgcac agcccgttta atcagcgatg gagccccggc cgtcagccag 840ccaggtccgg cgtccgggtc tgcgccctgc ggcgtcactg ctgtcgccac cgtctccgat 900ggtcccacat ccatccagcg ggccgcgcgt ggtacaaaag gctcttcctc gccgtcaggt 960gcagctgccc aaacaccaga cacagactcc accaccccgc ttcgatcttc tgttgcagct 1020gaaatctgtc agattctgca gttcattcct catga 1055161752DNAartificial sequenceZMCAS1BAMPRO fragment comprising 1.7 KB ZmCAS1 promoter 16ggatccgggg cggaagatgg cagggacgcg gattcagggc ggacgcgctt gccgagggcg 60cgggggacca cagcgtgcgt tacggggaca gggcgggcat cgcgaggacg ggtgcgggag 120cggagccaca tctggtggtg gacgcctact ttgctctctt atagagtagt aaagattcgt 180ggaccaaaca acaccctagc ttgtacaaat attcttaggc agttgctact gatgagagaa 240aaataacatc actccactgc atttgcgtga tttattgaac agatcacaat tacatctatt 300caaatttatt tacctgtacg tgtccgattt ttaggggagg atttttttac ggtatttttt 360ttttaaaaaa ataaatttag gcaacaattt tatagaatcg agtgctttat ctattatctt 420ttacaaggca cacgcgtaca ataaggtttg gtcgttcgtg acttggatag tggttttggt 480tgcaattccg taattcttgg cataggatac agcccaaccc agaaaaaaat aatgttgcgg 540tcagttctgg ctttgagatt cggagtacca cgtggcgtaa aggcaggccg tgtcttacag 600atgaataaag gacctgggtc tcacgtgatt ggtttccagt ttcgtgcatc aagatgtgga 660attttcaaac tgccgtcgtg tttgtttcgt cacataaaag ctttttggaa ggctaaggag 720aggaagccgg cgagaaggag ggggcgtttt acgtgtcact gtcctgtcgt gttggctgtt 780gacacgaatc atttcttccg cgcgtgggaa gaagaagatg cacattagcg gcctgaagta 840gagatgtcaa tggggaattc cccagcgggg attaactccc cagacccgta cccatgaaca 900tagaccggcc cccatccccg aacccgaacc cgacctcggg tacgaaaatc ctcccatacc 960cattcccgac cgggtactaa atacccatgg gtatccatac ccgacccgat tattcaaaaa 1020ttaatgggct ttttatttgt taaccggcgg acgcaatgct tgggactcta ggttttttta 1080ctttgttgac cggctggcgg ctgggctttt tcctacaggc ccaaagttgg tcggcagcca 1140ctaggccaca cgtcacaggc agcccacaag taaatgtcgt tggattgctg gatggtggaa 1200taaaaatcct agatgctaga ttgttctggt tccgggtatt tttctccatg gctaatcggg 1260tttgggttta gccctcccaa acccgaaccc gccatacccg atgggtaagg gatttattcc 1320aaatctatac ccatggggat ttgttttaac ccatacctta accctaatag aggaattccc 1380cacgggtaat cgggtttcgg ggcccattga catctctaga ctgaaggcgt ccaactcaaa 1440tcattaaaaa gtgttgacgc acgcgctgat gcgccggccg cacagcacag gctgcacagc 1500ccgtttaatc agcgatggag ccccggccgt cagccagcca ggtccggcgt ccgggtctgc 1560gccctgcggc gtcactgctg tcgccaccgt ctccgatggt cccacatcca tccagcgggc 1620cgcgcgtggt acaaaaggct cttcctcgcc gtcaggtgca gctgcccaaa caccagacac 1680agactccacc accccgcttc gatcttctgt tgcagctgaa

atctgtcaga ttctgcagtt 1740cattcctcat ga 175217354PRTOryza sativa 17Met Ala Ala Glu Cys Gly Ser Gly Asn Cys Asp Ala Trp Ala Ala Arg 1 5 10 15 Asp Pro Ser Gly Ile Leu Ser Pro Tyr Lys Phe Asn Arg Arg Ala Val 20 25 30 Gln Ser Asp Asp Val Ser Leu Arg Ile Thr His Cys Gly Val Cys Tyr 35 40 45 Ala Asp Val Ala Trp Thr Arg Asn Ile Leu Asn Asn Ser Met Tyr Pro 50 55 60 Leu Val Pro Gly His Glu Ile Ala Gly Val Val Thr Glu Val Gly Ala 65 70 75 80 Asp Val Lys Ser Phe Lys Val Gly Asp His Val Gly Val Gly Thr Tyr 85 90 95 Val Asn Ser Cys Arg Asp Cys Glu Asn Cys Asn Ser Ser Leu Glu Asn 100 105 110 Tyr Cys Ser Gln His Val Phe Thr Phe Asn Gly Val Asp Thr Asp Gly 115 120 125 Thr Val Thr Lys Gly Gly Tyr Ser Thr His Ile Val Val His Glu Arg 130 135 140 Tyr Cys Phe Lys Ile Pro Asp Gly Tyr Pro Leu Glu Lys Ala Ala Pro 145 150 155 160 Leu Leu Cys Ala Gly Ile Thr Val Tyr Ser Pro Met Met Arg His Asn 165 170 175 Met Asn Gln Pro Gly Lys Ser Leu Gly Val Ile Gly Leu Gly Gly Leu 180 185 190 Gly His Met Ala Val Lys Phe Gly Lys Ala Phe Gly Leu Lys Val Thr 195 200 205 Val Ile Ser Thr Ser Glu Ser Lys Arg Lys Glu Ala Ile Asp Leu Leu 210 215 220 Gly Ala Asp Asn Phe Val Val Ser Ser Asp Glu Asn Gln Met Glu Thr 225 230 235 240 Leu Lys Ser Ser Leu Asn Phe Ile Ile Asp Thr Ala Ser Gly Asp His 245 250 255 Pro Phe Asp Pro Tyr Leu Thr Leu Leu Lys Val Gly Gly Val Met Ala 260 265 270 Leu Leu Ser Phe Pro Ser Glu Ile Lys Val His Pro Ala Asn Leu Asn 275 280 285 Leu Gly Gly Arg Ser Leu Ser Gly Ser Val Thr Gly Gly Thr Lys Asp 290 295 300 Ile Gln Glu Met Ile Asn Phe Cys Ala Ala Asn Lys Ile Tyr Pro Asp 305 310 315 320 Ile Glu Met Ile Lys Ile Asp Tyr Ile Asn Glu Ala Leu Gln Arg Leu 325 330 335 Val Asp Arg Asp Val Arg Phe Arg Phe Val Ile Asp Ile Glu Asn Ser 340 345 350 Phe Lys 186254DNAOryza sativa 18cagtcacaaa ttttttggta attccgagaa aatttccgga aactccgaga acatttccag 60aaagtttccg accatttccg agttccgacg gaaactgccc ttatcatttc cgattccgtt 120tccgagaaaa tattttcgaa ttcgtttccg tttccgaaaa atccgaaaaa attctgaccg 180acagattccg tttccgaaaa taggtccgga atccggaaag tttccgtacc gttttcaccc 240ctaactggga caaaatgatt attttagtct aacatacatt gttaagtata ttaaagtcta 300cggtctatct cacagcttac aaatggtgag atatgatatc ttttaacatt atttcataaa 360acagtaaata agatgcccgg catcaagggg tgaaaacgat attgaaattt cccgaccata 420ccgataccgt tttcggaaat ctaatataat aataatattt ttaccaatgg ctaccatttt 480caaaatttag tttttttcta atttctatca gcgtggcatt gaagttaaca ctaaatatat 540gaattcgtaa acattaaaat tttccgaccg tttcggttcg ttttcgaaaa aataataaaa 600taatgatatc gtttccgacc atttctgata gttttcatcc ctaacggcat ccacgtgggc 660tttcttccta gttgagtaaa aagtggagta ttgagagttt acacgagaga attattagtg 720agattaagat ggaaatttga tttttagtcc taatatcttg tttagtaaat atggcggaaa 780ttgacaggga gtttcgttgg agattatgtc attaataaaa acggatggct gagatttgtt 840tatgcaaatt aaaggaggaa ttccccctat ccacgtattg aaagaggtgt tccttactca 900attctccatt cccatctcat taaaattatc gtgttttcca actaaacaaa acaaacaaaa 960cagataactt atccatctaa agttctaaac acctaatcac tcctcaaaaa ctacctccaa 1020ccaaaacagg gccgtaaagg aaaatcttcc gtgtcgtcgt ctcctccgct ccttgcgcca 1080agacgagtcg cggctcaaca gcggagaggc ggcgatctcc catctggcga gcagagcagg 1140ggaggggaag ggatcttggt gagcatccac atcctcttcc tgactcatct ctctcccacc 1200gggagtactt ctgtctggaa tttgcttgcg ttaaccctag cttctgttct aggtttggaa 1260gaagctcttc tcttaatttc agagccttaa tcttaataca agtgacagtt tgtttgttcc 1320ccaaaaagct gaatccgccc ctgtccagtg gtacgaattc agtttctgta gctgccagaa 1380gaagtaaatt aaatttcatg ttataccatg ttctgaaact ctcaagaatg ttgatggaag 1440ttgatggggt tctagtgtat aaaactggat cagtatttct ggttattgat tgggtttcac 1500aaactgtgga agtataaatt ggatattata gagtacaagg agcagagatg gggcctaaca 1560catgacacgt gcgttacaaa ggagaactgg agaagaggtg gattccttct caaattcccc 1620gcccacagat tcccatctcc aacctccatc tccgtctcct cagagatcca catagcgact 1680cccgatccag actaagcttg caaaactccc ttgccccagc agcgtcgaca gtgtggtgct 1740gaccggcggc ttctgatcaa aatagtcagt tggattggtc ctgtcctgtc ctgtcctggt 1800cctgaacctc taatctcttc atttcaaaac gaatagatga ctggattcat tttcctgcat 1860tttaaactac tggtttttct ttttgttgtt tttgacattg ttcattgctg atataagtat 1920caggggtata agattatgat ggatttatgg cattatgggt tcatctgagt tgattttttt 1980ctatctgcag ttagagtggc atggctgctg aatgtggaag tggcaactgt gatgcttggg 2040cagcgagaga tccttcaggg atcctctccc cgtacaagtt caaccgcagg ttaaatcaca 2100ctacactccc ttgtgtattg attttccttt ttattttctt gatatgaatg tctttccagc 2160actgttaaga aatcttcctt gtttccaagt gttttctcta actgcagaat gcactattct 2220ggcaactaga atcatcacga ttgcagtttc agttagctgt gtgcttatct ggaacaagaa 2280tgtgaacttg tcaagttacc tgttttgaat tttctaactt ttgcgcaaca tgataaactg 2340gattacaaaa tctctatagt actttgctga atgttgtgat taactactca cagacaagat 2400tttaaaatga tcttttccat ttttagtacc tattcttatg aattgttaat aacctggttt 2460attattacca gggctgtaca aagtgacgat gtttccttga ggatcacaca ctgtggtgtt 2520tgttatgctg atgttgcatg gacaaggaat atactcaaca attcgatgta ccctttagtc 2580cctgggtaaa attattaact ttgttacttg aattttaaca aaagtatgga aaagaataaa 2640cttctgttac agactataaa ttcatccatt tagctactgt atttgttgta aagataaaag 2700ttcatgaaat tgtgtctaaa tttcaatatg agtaggcatg agatagcagg agttgtaact 2760gaggttggtg cagacgtcaa gagcttcaaa gtgggtgacc atgtaggtgt tggcacatac 2820gtgaattcat gccgggactg tgagaactgc aatagctctc tagagaacta ctgctcacaa 2880catgtcttca ctttcaatgg tgttgacact gatgggactg tcacaaaggg aggatattct 2940actcacatag tagtacatga gaggtatgga ttttgaccat gttttcttct gaaagttttc 3000tgacaagaca acgaaaaatt tcatatactt atttctaatg gttgctcaac aatgttgtca 3060caaaatcatc ctacattctg ctatgtaaat tttatatagg aaacaaatcc catgatgttc 3120tgctaatgtc tctcttatga atatagcagc tattagttat tcctgtttac ttaattcaaa 3180aaagaaccta tgagaaaatt gcatcttcct ttggaatggc aggtattgct ttaaaatacc 3240tgatggctac cctttggaaa aggcagcacc tttactttgt gctggcatca ctgtatatag 3300tccgatgatg cggcataata tgaaccagcc agggaagtca ctcggcgtca ttggacttgg 3360tggcttgggt cacatggcag taaaatttgg gaaagccttt ggactgaaag tcacagttat 3420tagtactagt gaatcaaaga gaaaagaagc tattgacctt cttggtgcag ataatttcgt 3480ggtgtcatcg gatgaaaatc agatggaggt aatacacatt ctacattatg ttttacccca 3540ttgttcacag ttatctacta tgaccatacc atgagtctta tcagtcaaat gccatttggt 3600aaagatagct aacctgtact tctcatttat cttacgtact gaaagtggta atgacagatt 3660cttcattgtt gcagaccttg aaaagctctc tgaacttcat tattgacaca gcctccggcg 3720atcacccatt cgatccttat ctcacgcttc tgaaagttgg tggtgtaatg gcactactta 3780gcttcccaag tgaaatcaaa gtgcatcctg caaaccttaa tctcggtaat gcacgtttct 3840cataccaaaa tatatatggc tttttccaga gaggaaatat atctgtctgc taggatgggg 3900agaaaattaa ttaattacaa ctaatctgga ctcatgtagg tgggcggagt ttatctggta 3960gtgtaactgg aggtacgaag gacatccagg agatgataaa cttctgtgcg gcaaacaaaa 4020tctacccaga tatcgagatg atcaagatag attacatcaa cgaggctctt cagaggcttg 4080ttgaccggga tgtcagattt cgctttgtaa tcgacattga gaactcgttc aagtagtatc 4140ttgatatctt cagtacacca ttaatgatag aagtgtatgc aataataata agtttaaatg 4200tcctacaaga tgatgagacc atgagacagt tccagaacaa aatgttccat tttaagaagc 4260aatttggttc tgttttcagt tattttcagg agtaaattgt attggtggta cacaaactta 4320tttggtgggt gtaatttaat acataaactt gtttgttggg tgtaatttag tatataaatt 4380tgtttgttgg gtgtagttta gtacataaac taaacttgtg aagtacttat tttggtacat 4440gaacttatct aattcgtatg aatcacaatc aaaatggctt ggcaaagttg atccttcaaa 4500gttgattttg attaggatat agcatgcaca agtggtgagt acgcataaga tatgctatat 4560ttataaactt tgtttctact tttcatcatc attaaaatga tgaatttcct atgtttctat 4620ctcttgttaa atggttttca tatatttttc tataatcatt atgtctcgat ctatcttttt 4680attgaacata ctagagaaaa tgcctgtgcg ttgcaacggg tgaaaatgtt tgtggatcgc 4740tatatattat acttctttta ggttttgggt cgaaagctca tcccatcggc cccaacccag 4800gctttgagcc gaaagctcat ctcattgagc ccaacccaat ccaatatttc cctttgggcc 4860tcatcgggcc gaaagctcat ctcatcggtc cctacccagg ctttgggccg aaagctcatc 4920tcatcggtcc ctacctaggc tttgggccga aagctcatct catcgggccc aacccagtcc 4980gatatttccc tttgctcgac ttctctcttc ggctgatatt tccctttgtt cgacttcggt 5040tcaaacctgg agctactggc ccgactattt ccatttgttc gacttcggtt caaacctgga 5100gctgcaggcc cgactacgtc catcaatacc catctcttcc ttctttttca acccaacccg 5160cgatattttc gctgactatt gtccatcaat acccatctct tccttctttt ttaagcacaa 5220cccagtccgt ctcattcctt cctttttcaa cccaatctag tccgatattt ccttttgctc 5280gacctttttc agccgaaccc agttcgatat tttcctctac tcaacctttc tcttcctccc 5340ctccaggccc gactacgtcc atcaataccc atctcttcct tctttttcaa cccaacccgc 5400gatattttcc ccgactattg tccatcaata cccatctctt ccttcttttt taagcacaac 5460ccagtccgtc tcatcccttc ctttttcaac ccaatctagt ccgatattaa tacccatctc 5520ttccttcttt ttcaacccaa cccgcgatat tttccccgac tattgtccat caatacccat 5580ctcttccttc ttttttaagc acaacccagt ccgtctcatc ccttcctttt tcaacccaat 5640atagtccgat atttcctttt gctcgacctt tttcagccga acccagttcc atattttcct 5700ctactcaacc tttctcttcc tcccatcgtt aacccagtcc gatactttcc tctgctcgac 5760ctttctcttc ctcccatcat tcgatcgagc ccatctctcc ctttattttc agtccaatcc 5820agtccgatat ttctgcttgc tcaacctttt tcagcccaat ccagtccaat atttccgtct 5880gcttgacctg tttcttgctc ccatcatttg atcaagccca tctattactt tattttcagc 5940ccaacccagt ccgatatttc cctctactcg actattttca acccagttcg atatgtccct 6000ttgctcagac ctttttcagc ccaacccagt tcactatctc tcttcggctc aaaccacaag 6060tggagctaca ggtctagcga ctgtcatctt caacctacga aagacattcc cgtgcacata 6120cgatgtcgaa gtcgttcctc acctcaataa ttccatgtct taccgtgcac atgcgatgtt 6180ggagccgatc ctcccatcaa ttacgataaa atatttcttg atctcccaaa attggtctct 6240cttcggctca aacc 6254192000DNAOryza sativa 19cagtcacaaa ttttttggta attccgagaa aatttccgga aactccgaga acatttccag 60aaagtttccg accatttccg agttccgacg gaaactgccc ttatcatttc cgattccgtt 120tccgagaaaa tattttcgaa ttcgtttccg tttccgaaaa atccgaaaaa attctgaccg 180acagattccg tttccgaaaa taggtccgga atccggaaag tttccgtacc gttttcaccc 240ctaactggga caaaatgatt attttagtct aacatacatt gttaagtata ttaaagtcta 300cggtctatct cacagcttac aaatggtgag atatgatatc ttttaacatt atttcataaa 360acagtaaata agatgcccgg catcaagggg tgaaaacgat attgaaattt cccgaccata 420ccgataccgt tttcggaaat ctaatataat aataatattt ttaccaatgg ctaccatttt 480caaaatttag tttttttcta atttctatca gcgtggcatt gaagttaaca ctaaatatat 540gaattcgtaa acattaaaat tttccgaccg tttcggttcg ttttcgaaaa aataataaaa 600taatgatatc gtttccgacc atttctgata gttttcatcc ctaacggcat ccacgtgggc 660tttcttccta gttgagtaaa aagtggagta ttgagagttt acacgagaga attattagtg 720agattaagat ggaaatttga tttttagtcc taatatcttg tttagtaaat atggcggaaa 780ttgacaggga gtttcgttgg agattatgtc attaataaaa acggatggct gagatttgtt 840tatgcaaatt aaaggaggaa ttccccctat ccacgtattg aaagaggtgt tccttactca 900attctccatt cccatctcat taaaattatc gtgttttcca actaaacaaa acaaacaaaa 960cagataactt atccatctaa agttctaaac acctaatcac tcctcaaaaa ctacctccaa 1020ccaaaacagg gccgtaaagg aaaatcttcc gtgtcgtcgt ctcctccgct ccttgcgcca 1080agacgagtcg cggctcaaca gcggagaggc ggcgatctcc catctggcga gcagagcagg 1140ggaggggaag ggatcttggt gagcatccac atcctcttcc tgactcatct ctctcccacc 1200gggagtactt ctgtctggaa tttgcttgcg ttaaccctag cttctgttct aggtttggaa 1260gaagctcttc tcttaatttc agagccttaa tcttaataca agtgacagtt tgtttgttcc 1320ccaaaaagct gaatccgccc ctgtccagtg gtacgaattc agtttctgta gctgccagaa 1380gaagtaaatt aaatttcatg ttataccatg ttctgaaact ctcaagaatg ttgatggaag 1440ttgatggggt tctagtgtat aaaactggat cagtatttct ggttattgat tgggtttcac 1500aaactgtgga agtataaatt ggatattata gagtacaagg agcagagatg gggcctaaca 1560catgacacgt gcgttacaaa ggagaactgg agaagaggtg gattccttct caaattcccc 1620gcccacagat tcccatctcc aacctccatc tccgtctcct cagagatcca catagcgact 1680cccgatccag actaagcttg caaaactccc ttgccccagc agcgtcgaca gtgtggtgct 1740gaccggcggc ttctgatcaa aatagtcagt tggattggtc ctgtcctgtc ctgtcctggt 1800cctgaacctc taatctcttc atttcaaaac gaatagatga ctggattcat tttcctgcat 1860tttaaactac tggtttttct ttttgttgtt tttgacattg ttcattgctg atataagtat 1920caggggtata agattatgat ggatttatgg cattatgggt tcatctgagt tgattttttt 1980ctatctgcag ttagagtggc 200020354PRTSorghum bicolor 20Met Ala Ala Glu Ser Glu His Gly Asn Cys Asn Ala Trp Ala Ala Arg 1 5 10 15 Asp Pro Ser Gly Val Leu Ser Pro Tyr Ser Phe Asn Arg Arg Pro Val 20 25 30 Gln Ser Ser Asp Val Ala Leu Lys Ile Leu Tyr Cys Gly Val Cys Tyr 35 40 45 Ala Asp Val Val Trp Thr Arg Asn Met His His Asp Ser Lys Tyr Pro 50 55 60 Val Val Pro Gly His Glu Ile Ala Gly Val Val Thr Gln Val Gly Ala 65 70 75 80 Asp Val Lys Gly Phe Lys Val Gly Asp His Val Gly Val Gly Thr Tyr 85 90 95 Val Asn Ser Cys Arg Asp Cys Glu Asn Cys Asn Ser Ser Leu Glu Asn 100 105 110 His Cys Pro Lys Gly Val Tyr Thr Phe Asn Gly Ile Asp Thr Asp Gly 115 120 125 Thr Val Thr Lys Gly Gly Tyr Ser Thr His Ile Val Val His Glu Arg 130 135 140 Tyr Cys Phe Gln Ile Pro Asp Gly Tyr Pro Leu Ala Lys Ala Ala Pro 145 150 155 160 Leu Leu Cys Ala Gly Ile Thr Val Tyr Thr Pro Met Met Arg His Asn 165 170 175 Met Asn Gln Pro Gly Lys Ser Leu Gly Val Ile Gly Leu Gly Gly Leu 180 185 190 Gly His Met Ala Val Lys Phe Gly Lys Ala Phe Gly Leu Lys Val Thr 195 200 205 Val Leu Ser Thr Ser Glu Ser Lys Arg His Glu Ala Ile Ser Leu Leu 210 215 220 Gly Ala Asp Asn Phe Val Ile Ser Ser Asp Thr Gln Gln Met Glu Ser 225 230 235 240 Leu Arg Asn Ser Leu His Phe Ile Val Asp Thr Ala Ser Gly Asp His 245 250 255 Pro Phe Asp Pro Tyr Leu Ser Leu Leu Met Val Gly Gly Val Met Ala 260 265 270 Ile Val Gly Phe Pro Ser Glu Ile Lys Met His Pro Ala Ser Leu Asn 275 280 285 Leu Gly Ala Arg Thr Leu Ser Gly Ser Val Thr Gly Gly Thr Lys Asp 290 295 300 Ile Gln Glu Met Val Asn Phe Cys Ala Ala Asn Lys Ile Ser Pro Glu 305 310 315 320 Ile Glu Ile Ile Lys Ile Asp Tyr Ile Asn Glu Ala Leu Thr Arg Leu 325 330 335 Val Asn Arg Asp Val Lys Tyr Arg Phe Val Ile Asp Ile Glu Asn Ser 340 345 350 Phe Lys 215242DNASorghum bicolor 21aaaagtcaaa cgtcttataa tttgggatgg agtattaaac atgcataaac ctaggtaaat 60taatatttca atcatacatg gtccaatgag tcttaatttt ttagcatagc taaagcatac 120aattagtagc ctaccataaa agttttacca caatcaaagc acaaagagct acaattatga 180attaaacaca aaaacacata tatctacagc tacaacaaaa atgttaaact aaagcatgtc 240atattatata tctattatat agagcttgtc atgtggagtc taacaaaact gaattttcgt 300ttttacaaat tttctatgat tttatagaat ttttttaaag ttttagtcag tttatgaaat 360aaaaaagagc atgtgggcca catcagcaac cacattggca ggagagtcct tcgtgattgg 420atggtggcta ggttctagac aaagtttatg gatctagatg agcgaaaaaa gtttatggac 480atatgtgaga agtggagaaa agtttaagaa ccctcaacac atttgactca agaaagaata 540acataaaacc aaccacacac taaatttaag gttgaaatcc aaatgatatg atatatgatg 600aaatacaaac attcatgaga tttttataaa agaataaaga catagtaaat aaagcaaaga 660atatataaaa aataagtata tagttatctg ttgacaaatt ttatatagta tattttataa 720atacgaacta gtagatagtt ctagccaatc ctaaaacata atcaaacaca tagaaatcta 780acatatttaa ggggtgtttg gttggaggtg ttaaagttta atatgtatta taacattttc 840gttttatttg acaattagtg tctaatcatt gactaactag gcttaaaaga tttgtctggc 900aaattacttt ctagttatgt ttttagtttc ataaataatc tatatttagt actttatgca 960tatgtccaaa cattcgatgt gacgagagtt aaagtttaac tatggaaacc aaggccctta 1020ctaaagaaat ctgttaacag atcaataaca gaagtcatat aaaaatcaaa tatcagaaaa 1080taatgatcta tctatattat gaaaaaaaca agcgttcatg agtttgagtt ttttttaccg 1140tgtttgttat ttactaaaag aaaactacaa gtacttttat ctgttctatt tacaaggcac 1200gggtctaatt ataagtttct tttacctttg aaattcttaa tattccgacg gtagtattag 1260tcgtatgata caatactccc attcggccat tccattagct gaggccttgt ttagttccta 1320aaaagttttg ccaaattttc agatttttcg tcacatcaaa ttttacggca tatgcatgaa 1380acattaaata tagataaaaa gaataactaa ttacatagtt taactgtaat ttgtgagacg 1440aatttttaaa atctaattaa tctataatta aataatattt gtcaaataca aacgtggtat 1500aatgcctatt ttactaattt ttttgaaact acttcctccg tcccataaat aattgcattt 1560ctttaacttc tatagtttat gtttgaccgt tcgtcttatt aaaaaatttt taataaatat 1620tatttatttt ttcatgactt attttattgt tagatatatt ttttatgata atttatttat 1680tttattattt gtacaaaaat ttaaataaaa taaatggtca aacattacta ttagcagtcg 1740aagaaatgca tttatttatg ggacgaagag agtaaacaag gcctgaagct gaagcctgaa 1800ggaagaaagg gaaaggggat tgacgcatgc actgatggtt cggccaggca caatcagcga 1860tgaactcatg gctgacgaga agccccgtaa ctccggaagc caggtccgcg acgtcactgc 1920tgtcggcacc

ctctccaatc caatccatcc acccgtcaac ggagcatgat ccggtctgcg 1980cccttccaac ttcttcccca ttataatttg ctcactcgcc ggcggccgct acctgctgca 2040acccggcgac acccgaccga cagcctgctt gccacgcttg catcgaggag agttcgggga 2100ccgcccggtg cagaaggaat cgacgcaggg gtgagtcttt tcccctcccc tcccatgtac 2160ttcccgcctc gattcgactc caagggtcag cgatttcact gcgcttttcc ttagcatagg 2220ctatctgaaa tttccttctg ttagagccga gtctttcgat ctgctgactt gtcacgaatt 2280cattctgcag ttcagttcga atggctgctg aatcagagca cggcaactgc aatgcttggg 2340cagcgagaga tccttctgga gttctctcac catacagctt taaccgcagg tggcttccca 2400ccccccccca cccccccccc cccccccccc cgcgtctcct gatctggaga aaagttgcgt 2460catgtttagc actctgaata ctgtattctt gctatgctag actgtttctt tgggatagct 2520atggaccgta ttgtttccat gtctttctgt gtacacgcta tatgtttggt tttcagtgtg 2580tttcatacct gttatcaaat gagcaatatc ttggtgtgga gcaagtagtg cagctatttc 2640tgctcgtggt ctgtcaaaca aaatcatgaa attgatgctt actatgggca ttgggtgaac 2700attttaggtc tgatgggatt gcagaatttt gttcaacgtt ggcaggatct gccttttatt 2760attaaaaaaa taagggaagc gacctaagtc aacaactata cagattgatt gcagaaattt 2820gagtacaaat agcaacgtac ttgtcttttt gttcagctag cttgtctgtt tcatttttta 2880taacgtcttc ttatgagagc tactatgtgt aataattttc agaccagtgc aaagcagcga 2940tgttgcgttg aagatcttat actgtggagt ctgttatgct gacgttgtct ggacacggaa 3000tatgcaccat gattcaaaat atcctgtggt tcctgggtaa gcgtctgaca acaatttaga 3060cttgtatcag ggaagggcat gtttcttatc aatcgcgaat tgcatttcaa gatccaactc 3120tgaattgacc aggactagta gttttactag gtgttctaat ctaaggggca gaatatgact 3180aatagttaca gactaacagc ccagatcaat acattgaacc cctctagagc aaacaaaaca 3240tcatagtcag gagtatcgat taagtgtttg cccattgtcc aaaaatgatg tctgtgtact 3300atatatttgt caatgatatt attcattggt gcaggcatga gatagctgga gtcgtaactc 3360aggttggtgc agatgtcaaa ggctttaaag tgggtgacca tgtaggtgtt ggaacttatg 3420tgaactcatg ccgagattgt gagaactgca atagctctct agagaaccac tgtccaaaag 3480gagtttatac tttcaatggc attgatacag atggaactgt cacaaagggg ggttactcca 3540ctcacattgt agtccatgaa aggtatggca gtactgtttt acttggctgt tcaaacaaga 3600ctctttttat attgacaaaa gctattttag ggatgaaaac aggttccact gtgatttcat 3660ttgcctattt aatcacactg tgtcgtgttc aagatgttat atacttctgt atgaaagtaa 3720actctgggtc attgctgtcc ataaattttc atcacaatca atacatgatc aaagaattat 3780atatgggtct atataagtca ttattgacac cttgatgctg attcgataca acctgtcaga 3840taagggatgg catcttctgt tgattaattt gaactttagg tattgcttcc aaatacctga 3900tggctatcct ttggcgaagg cagcacctct tctgtgtgct ggaataactg tgtatactcc 3960aatgatgcga cacaacatga accaacctgg aaagtcactt ggggtcattg gactcggtgg 4020tctaggtcac atggcagtga aatttggtaa agcatttggt ctgaaggtca cagttttgag 4080tacaagtgaa tcaaagagac atgaagccat cagcctcctt ggagcggata attttgttat 4140atcatcagat acacagcaga tggaggtact gctctggaca ttgcgtaatc tgaacgtacc 4200taggcacctc gtacttcatc tgttccgaat atgagaaatt ttatttttgt cctaagtcaa 4260aaaactctat aattgaccta gtttatagaa aaaagcacta gtatctacaa gaccaaattt 4320attccattga atctcctgta gaatgtgttt ggtattgtag atgtcaatat atttttctat 4380aaacttagtc aaagctagac aaaattgact taggataaaa ctaaaacatc ttacatttga 4440aagggagaga atataccacg tttcccttct tgattatatg acaccatatt ttggtggggt 4500tctttttgtt tctttatttt attgccaaag atgcaagcac atcacattgt tttcacatgg 4560aacgaacaga tagcagatca ctttcgttct cattaatttg ctacttgact ttaaagttcc 4620aggcagccag ttcagtatat gacattggat agttttctta tgctgagaat aaagataaca 4680gctaagtact ggctgttctg ctttacctga aaactgaccg tgaatatatg atctcttgct 4740tttgcagtcc ctgagaaact ccctgcactt catagttgac accgcttctg gcgaccatcc 4800atttgatcca tatctctctc tccttatggt tggtggtgtg atggcaattg tgggctttcc 4860aagtgagatc aaaatgcatc ctgcaagcct taatcttggt aattctctgt tctgacactc 4920tgtactaaaa agaaacgtgg tattagaagc caaattattc agatttaacc tgaactgttt 4980tttaggtgca cggaccttgt ctggtagtgt tactggaggt acaaaagaca tccaagaaat 5040ggttaacttc tgtgcggcga acaaaatctc tccagagatt gagatcatta agatagatta 5100tatcaatgag gctctcacga ggcttgttaa ccgagatgtg aaataccgct ttgttatcga 5160catcgagaac tctttcaagt aacatgctgg tctacatgct ctcagtcttt ctattaatat 5220actagagcaa acacaaatgt ga 5242222300DNASorghum bicolor 22aaaagtcaaa cgtcttataa tttgggatgg agtattaaac atgcataaac ctaggtaaat 60taatatttca atcatacatg gtccaatgag tcttaatttt ttagcatagc taaagcatac 120aattagtagc ctaccataaa agttttacca caatcaaagc acaaagagct acaattatga 180attaaacaca aaaacacata tatctacagc tacaacaaaa atgttaaact aaagcatgtc 240atattatata tctattatat agagcttgtc atgtggagtc taacaaaact gaattttcgt 300ttttacaaat tttctatgat tttatagaat ttttttaaag ttttagtcag tttatgaaat 360aaaaaagagc atgtgggcca catcagcaac cacattggca ggagagtcct tcgtgattgg 420atggtggcta ggttctagac aaagtttatg gatctagatg agcgaaaaaa gtttatggac 480atatgtgaga agtggagaaa agtttaagaa ccctcaacac atttgactca agaaagaata 540acataaaacc aaccacacac taaatttaag gttgaaatcc aaatgatatg atatatgatg 600aaatacaaac attcatgaga tttttataaa agaataaaga catagtaaat aaagcaaaga 660atatataaaa aataagtata tagttatctg ttgacaaatt ttatatagta tattttataa 720atacgaacta gtagatagtt ctagccaatc ctaaaacata atcaaacaca tagaaatcta 780acatatttaa ggggtgtttg gttggaggtg ttaaagttta atatgtatta taacattttc 840gttttatttg acaattagtg tctaatcatt gactaactag gcttaaaaga tttgtctggc 900aaattacttt ctagttatgt ttttagtttc ataaataatc tatatttagt actttatgca 960tatgtccaaa cattcgatgt gacgagagtt aaagtttaac tatggaaacc aaggccctta 1020ctaaagaaat ctgttaacag atcaataaca gaagtcatat aaaaatcaaa tatcagaaaa 1080taatgatcta tctatattat gaaaaaaaca agcgttcatg agtttgagtt ttttttaccg 1140tgtttgttat ttactaaaag aaaactacaa gtacttttat ctgttctatt tacaaggcac 1200gggtctaatt ataagtttct tttacctttg aaattcttaa tattccgacg gtagtattag 1260tcgtatgata caatactccc attcggccat tccattagct gaggccttgt ttagttccta 1320aaaagttttg ccaaattttc agatttttcg tcacatcaaa ttttacggca tatgcatgaa 1380acattaaata tagataaaaa gaataactaa ttacatagtt taactgtaat ttgtgagacg 1440aatttttaaa atctaattaa tctataatta aataatattt gtcaaataca aacgtggtat 1500aatgcctatt ttactaattt ttttgaaact acttcctccg tcccataaat aattgcattt 1560ctttaacttc tatagtttat gtttgaccgt tcgtcttatt aaaaaatttt taataaatat 1620tatttatttt ttcatgactt attttattgt tagatatatt ttttatgata atttatttat 1680tttattattt gtacaaaaat ttaaataaaa taaatggtca aacattacta ttagcagtcg 1740aagaaatgca tttatttatg ggacgaagag agtaaacaag gcctgaagct gaagcctgaa 1800ggaagaaagg gaaaggggat tgacgcatgc actgatggtt cggccaggca caatcagcga 1860tgaactcatg gctgacgaga agccccgtaa ctccggaagc caggtccgcg acgtcactgc 1920tgtcggcacc ctctccaatc caatccatcc acccgtcaac ggagcatgat ccggtctgcg 1980cccttccaac ttcttcccca ttataatttg ctcactcgcc ggcggccgct acctgctgca 2040acccggcgac acccgaccga cagcctgctt gccacgcttg catcgaggag agttcgggga 2100ccgcccggtg cagaaggaat cgacgcaggg gtgagtcttt tcccctcccc tcccatgtac 2160ttcccgcctc gattcgactc caagggtcag cgatttcact gcgcttttcc ttagcatagg 2220ctatctgaaa tttccttctg ttagagccga gtctttcgat ctgctgactt gtcacgaatt 2280cattctgcag ttcagttcga 2300

Claims

1. A recombinant DNA construct comprising: a) a nucleotide sequence comprising any one of the sequences set forth in SEQ ID NO:9 or SEQ ID NO:10, or a full-length complement thereof; b) a nucleotide sequence comprising a functional fragment of SEQ ID NO:10, or a full-length complement thereof; c) a nucleotide sequence comprising a sequence having at least 85% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a) or (b); d) a nucleotide sequence which hybridizes to SEQ ID NO:9 under highly stringent conditions of a wash of 0.1 SSC, 0.1% (w/v) SDS at 65.degree. C.; e) a nucleotide sequence comprising all or a fragment of a 1.7 kb 5' non-coding sequence of a mannitol dehydrogenase; or, f) a derivative of one of the nucleotide sequences indicated in (a), (b), (c), (d) or (e) obtained by substitution, addition and/or deletion of one or more nucleotides; and, wherein said nucleotide sequence is an inducible promoter.

2. The recombinant DNA construct of claim 1, wherein the nucleotide sequence of c) has at least 90% identity, based on the BLASTN method of alignment, when compared to the sequence set forth in SEQ ID NO:1.

3-4. (canceled)

5. The recombinant DNA construct of claim 1 wherein said inducible promoter is induced by a chemical or stress treatment.

6. The recombinant DNA construct of claim 1 wherein said inducible promoter is induced by a safener or heat treatment.

7. (canceled)

8. The recombinant DNA construct of claim 6, wherein said heat treatment comprises a temperature greater than 26.degree. C.

9-11. (canceled)

12. A vector comprising the recombinant DNA construct of claim 1.

13. A cell comprising the recombinant DNA construct of claim 1.

14. The cell of claim 13, wherein the cell is a plant cell.

15. (canceled)

16. A transgenic plant having stably incorporated into its genome the recombinant DNA construct of claim 1.

17-20. (canceled)

21. Transgenic seed produced by the transgenic plant of claim 16.

22. A plant stably transformed with a recombinant expression construct comprising a plant promoter and a heterologous nucleic acid fragment operably linked to said promoter, wherein said promoter is an inducible promoter and capable of controlling expression of said heterologous nucleic acid fragment in a plant cell, and further wherein said promoter comprises a fragment of SEQ ID NO:10.

23. A method of expressing a coding sequence or a functional RNA in a plant cell comprising: a) introducing the recombinant DNA construct of claim 1 into a plant cell, wherein the at least one heterologous sequence comprises a coding sequence or a functional RNA; b) growing the plant cell of step a); c) induction of the inducible promoter by chemical or stress treatment on the plant cell of b); and, d) selecting a plant cell displaying expression of the coding sequence or the functional RNA of the recombinant DNA construct.

24-29. (canceled)

30. A method for altering expression of at least one heterologous nucleic acid fragment in a plant comprising: (a) transforming a plant cell with the recombinant expression construct of claim 1; (b) induction of the inducible promoter by chemical or stress treatment on the cell of (a) (c) growing fertile mature plants from the transformed plant cell of step (a); and, (d) selecting plants containing the transformed plant cell wherein the expression of the heterologous nucleic acid fragment is increased or decreased.

31. A method of transgenically altering a marketable plant trait, comprising: a) introducing a recombinant DNA construct of claim 1 into a plant; b) induction of the inducible promoter by chemical or stress treatment on the plant of (a); c) growing a fertile, mature plant resulting from step b); and d) selecting a plant expressing the at least one heterologous nucleotide sequence in at least one plant tissue based on the altered marketable trait.

32. (canceled)

33. A recombinant DNA construct comprising: a) a nucleotide sequence comprising all or a functional fragment of SEQ ID NO: 19 or SEQ ID NO: 22; b) a nucleotide sequence comprising a full-length complement of the nucleotide sequence (a); or, c) a nucleotide sequence comprising a sequence having at least 90% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a) or (b); and, wherein said nucleotide sequence is a promoter.

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