Transgenic Plant With Increased Stress Tolerance and Yield

Abstract

Polynucleotides are disclosed which are capable of enhancing growth, yield under water-limited conditions, and/or increased tolerance to an environmental stress of a plant transformed to contain such polynucleotides. Also provided are methods of using such polynucleotides and transgenic plants and agricultural products, including seeds, containing such polynucleotides as transgenes.

Description

FIELD OF THE INVENTION

[0001] This invention relates generally to transgenic plants which overexpress nucleic acid sequences encoding polypeptides capable of conferring increased stress tolerance and consequently, increased plant growth and crop yield, under normal or abiotic stress conditions. Additionally, the invention relates to novel isolated nucleic acid sequences encoding polypeptides that confer upon a plant increased tolerance under abiotic stress conditions, and/or increased plant growth and/or increased yield under normal or abiotic stress conditions.

BACKGROUND OF THE INVENTION

[0002] Abiotic environmental stresses, such as drought, salinity, heat, and cold, are major limiting factors of plant growth and crop yield. Crop yield is defined herein as the number of bushels of relevant agricultural product (such as grain, forage, or seed) harvested per acre. Crop losses and crop yield losses of major crops such as soybean, rice, maize (corn), cotton, and wheat caused by these stresses represent a significant economic and political factor and contribute to food shortages in many underdeveloped countries.

[0003] Water availability is an important aspect of the abiotic stresses and their effects on plant growth. Continuous exposure to drought conditions causes major alterations in the plant metabolism which ultimately lead to cell death and consequently to yield losses. Because high salt content in some soils results in less water being available for cell intake, high salt concentration has an effect on plants similar to the effect of drought on plants. Additionally, under freezing temperatures, plant cells lose water as a result of ice formation within the plant. Accordingly, crop damage from drought, heat, salinity, and cold stress, is predominantly due to dehydration.

[0004] Because plants are typically exposed to conditions of reduced water availability during their life cycle, most plants have evolved protective mechanisms against dessication caused by abiotic stresses. However, if the severity and duration of dessication conditions are too great, the effects on development, growth, plant size, and yield of most crop plants are profound. Developing plants efficient in water use is therefore a strategy that has the potential to significantly improve human life on a worldwide scale.

[0005] Traditional plant breeding strategies are relatively slow and require abiotic stress-tolerant founder lines for crossing with other germplasm to develop new abiotic stress-resistant lines. Limited germplasm resources for such founder lines and incompatibility in crosses between distantly related plant species represent significant problems encountered in conventional breeding. Breeding for tolerance has been largely unsuccessful.

[0006] Many agricultural biotechnology companies have attempted to identify genes that could confer tolerance to abiotic stress responses, in an effort to develop transgenic abiotic stress-tolerant crop plants. Although some genes that are involved in stress responses or water use efficiency in plants have been characterized, the characterization and cloning of plant genes that confer stress tolerance and/or water use efficiency remains largely incomplete and fragmented. To date, success at developing transgenic abiotic stress-tolerant crop plants has been limited, and no such plants have been commercialized.

[0007] In order to develop transgenic abiotic stress-tolerant crop plants, it is necessary to assay a number of parameters in model plant systems, greenhouse studies of crop plants, and in field trials. For example, water use efficiency (WUE), is a parameter often correlated with drought tolerance. Studies of a plant's response to desiccation, osmotic shock, and temperature extremes are also employed to determine the plant's tolerance or resistance to abiotic stresses. When testing for the impact of the presence of a transgene on a plant's stress tolerance, the ability to standardize soil properties, temperature, water and nutrient availability and light intensity is an intrinsic advantage of greenhouse or plant growth chamber environments compared to the field.

[0008] WUE has been defined and measured in multiple ways. One approach is to calculate the ratio of whole plant dry weight, to the weight of water consumed by the plant throughout its life. Another variation is to use a shorter time interval when biomass accumulation and water use are measured. Yet another approach is to use measurements from restricted parts of the plant, for example, measuring only aerial growth and water use. WUE also has been defined as the ratio of CO₂ uptake to water vapor loss from a leaf or portion of a leaf, often measured over a very short time period (e.g. seconds/minutes). The ratio of ¹³C/¹²C fixed in plant tissue, and measured with an isotope ratio mass-spectrometer, also has been used to estimate WUE in plants using C₃ photosynthesis.

[0009] An increase in WUE is informative about the relatively improved efficiency of growth and water consumption, but this information taken alone does not indicate whether one of these two processes has changed or both have changed. In selecting traits for improving crops, an increase in WUE due to a decrease in water use, without a change in growth would have particular merit in an irrigated agricultural system where the water input costs were high. An increase in WUE driven mainly by an increase in growth without a corresponding jump in water use would have applicability to all agricultural systems. In many agricultural systems where water supply is not limiting, an increase in growth, even if it came at the expense of an increase in water use (i.e. no change in WUE), could also increase yield. Therefore, new methods to increase both WUE and biomass accumulation are required to improve agricultural productivity.

[0010] Concomitant with measurements of parameters that correlate with abiotic stress tolerance are measurements of parameters that indicate the potential impact of a transgene on crop yield. For forage crops like alfalfa, silage corn, and hay, the plant biomass correlates with the total yield. For grain crops, however, other parameters have been used to estimate yield, such as plant size, as measured by total plant dry weight, above-ground dry weight, above-ground fresh weight, leaf area, stem volume, plant height, rosette diameter, leaf length, root length, root mass, tiller number, and leaf number. Plant size at an early developmental stage will typically correlate with plant size later in development. A larger plant with a greater leaf area can typically absorb more light and carbon dioxide than a smaller plant and therefore will likely gain a greater weight during the same period. This is in addition to the potential continuation of the micro-environmental or genetic advantage that the plant had to achieve the larger size initially. There is a strong genetic component to plant size and growth rate, and so for a range of diverse genotypes plant size under one environmental condition is likely to correlate with size under another. In this way a standard environment is used to approximate the diverse and dynamic environments encountered at different locations and times by crops in the field.

[0011] Harvest index, the ratio of seed yield to above-ground dry weight, is relatively stable under many environmental conditions and so a robust correlation between plant size and grain yield is possible. Plant size and grain yield are intrinsically linked, because the majority of grain biomass is dependent on current or stored photosynthetic productivity by the leaves and stem of the plant. Therefore, selecting for plant size, even at early stages of development, has been used as to screen for plants that may demonstrate increased yield when exposed to field testing. As with abiotic stress tolerance, measurements of plant size in early development, under standardized conditions in a growth chamber or greenhouse, are standard practices to measure potential yield advantages conferred by the presence of a transgene.

[0012] There is a need, therefore, to identify additional genes expressed in stress tolerant plants and/or plants that are efficient in water use that have the capacity to confer stress tolerance and/or increased water use efficiency to the host plant and to other plant species. Newly generated stress tolerant plants and/or plants with increased water use efficiency will have many advantages, such as an increased range in which the crop plants can be cultivated, by for example, decreasing the water requirements of a plant species. Other desirable advantages include increased resistance to lodging, the bending of shoots or stems in response to wind, rain, pests, or disease.

SUMMARY OF THE INVENTION

[0013] The present inventors have discovered that transforming a plant with certain polynucleotides results in enhancement of the plant's growth and/or response to environmental stress, and accordingly the yield of the agricultural products of the plant is increased, when the polynucleotides are present in the plant as transgenes. The polynucleotides capable of mediating such enhancements have been isolated from Physcomitrella patens, Brassica napes, Zea mays, Linum usitatissimum, Oryza satvia, Glycine max, or Triticum aestivum and are listed in Table 1, and the sequences thereof are set forth in the Sequence Listing as indicated in Table 1.

TABLE-US-00001 TABLE 1 Poly- Amino nucleotide acid Gene Gene SEQ SEQ Name ID Organism ID NO ID NO PpTPT-1 EST 214 P. patens 1 2 PpCDC2-1 EST 280 P. patens 3 4 PpLRP-1 EST 298a P. patens 5 6 EST 298b P. patens 55 56 EST 298c P. patens 57 58 PpRBP-1 EST 300 P. patens 7 8 PpPD-1 EST 362 P. patens 9 10 PpMSC-1 EST 378 P. patens 11 12 PpMBP-1 EST 398 P. patens 13 14 PpAK-1 EST 407 P. patens 15 16 PpZF-6 EST 458 P. patens 17 18 PpCDK-1 EST 479 P. patens 19 20 PpZF-7 EST 520 P. patens 21 22 PpMFP-1 EST 544 P. patens 23 24 PpLRP-2 EST 545 P. patens 25 26 PpPPK-1 EST 549 P. patens 27 28 PpSRP-1 EST 554 P. patens 29 30 PpCBL-1 EST 321 P. patens 31 32 PpCBL-2 EST 416 P. patens 33 34 PpHD-1 EST 468 P. patens 35 36 BnHD-1 BN51361834 B. napus 37 38 BnHD-2 BN50000854 B. napus 39 40 ZmHD-1 ZM59324542 Z. mays 41 42 LuHD-1 LU61552369 L. usitatissimum 43 44 OsHD-1 OS34631911 O. sativa 45 46 GmHD-1 GM59700314 G. max 47 48 GmHD-2 GM49753757 G. max 49 50 GmHD-3 GM50270592 G. max 51 52 TaHD-1 TA60089198 T. aestivum 53 54

[0014] In one embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a tRNA 2'-phosphotransferase.

[0015] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a cell division control protein kinase.

[0016] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a leucine-rich repeat protein.

[0017] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a Ran-binding protein.

[0018] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a plastid division protein.

[0019] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a mitochondrial substrate carrier protein.

[0020] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MADS-box protein.

[0021] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an adenosine kinase-1 protein.

[0022] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a zinc finger-6 protein.

[0023] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a cyclin-dependent kinase regulatory subunit protein.

[0024] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a zinc finger-7 protein.

[0025] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MAR-binding protein.

[0026] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a leucine rich repeat receptor protein.

[0027] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a phytochrome protein kinase protein.

[0028] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a synaptobrevin protein.

[0029] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a calcineurin B protein.

[0030] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a caleosin protein.

[0031] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a histone deacetylase protein.

[0032] In a further embodiment, the invention concerns a seed produced by the transgenic plant of the invention, wherein the seed is true breeding for a transgene comprising the polynucleotide described above. Plants derived from the seed of the invention demonstrate increased tolerance to an environmental stress, and/or increased plant growth, and/or increased yield, under normal or stress conditions as compared to a wild type variety of the plant.

[0033] In a still another aspect, the invention concerns products produced by or from the transgenic plants of the invention, their plant parts, or their seeds, such as a foodstuff, feedstuff, food supplement, feed supplement, cosmetic or pharmaceutical.

[0034] The invention further provides the isolated polynucleotides identified in Table 1 or in Table 2 below, and polypeptides identified in Table 1. The invention is also embodied in recombinant vector comprising an isolated polynucleotide of the invention.

[0035] In yet another embodiment, the invention concerns a method of producing the aforesaid transgenic plant, wherein the method comprises transforming a plant cell with an expression vector comprising an isolated polynucleotide of the invention, and generating from the plant cell a transgenic plant that expresses the polypeptide encoded by the polynucleotide. Expression of the polypeptide in the plant results in increased tolerance to an environmental stress, and/or growth, and/or yield under normal and/or stress conditions as compared to a wild type variety of the plant.

[0036] In still another embodiment, the invention provides a method of increasing a plant's tolerance to an environmental stress, and/or growth, and/or yield. The method comprises the steps of transforming a plant cell with an expression cassette comprising an isolated polynucleotide of the invention, and generating a transgenic plant from the plant cell, wherein the transgenic plant comprises the polynucleotide.

BRIEF DESCRIPTION OF THE DRAWINGS

[0037] FIG. 1 is a diagram illustrating the phylogenetic relationship among the disclosed PpHD-1 (SEQ ID NO:36), BnHD-1 (SEQ ID NO:38), BnHD-2 (SEQ ID NO:40), ZmHD-1 (SEQ ID NO:42), LuHD-1 (SEQ ID NO:44), OsHD-1 (SEQ ID NO:46), GmHD-1 (SEQ ID NO:48), GmHD-2 (SEQ ID NO:50), GmHD-3 (SEQ ID NO:52), and TaHD-1 (SEQ ID NO:54) amino acid sequences. The diagram was generated using Align X of Vector NTI.

[0038] FIG. 2 shows an alignment of the disclosed amino acids sequences: PpHD-1 (SEQ ID NO:36), BnHD-1 (SEQ ID NO:38), BnHD-2 (SEQ ID NO:40), ZmHD-1 (SEQ ID NO:42), LuHD-1 (SEQ ID NO:44), OsHD-1 (SEQ ID NO:46), GmHD-1 (SEQ ID NO:48), GmHD-2 (SEQ ID NO:50), GmHD-3 (SEQ ID NO:52), and TaHD-1 (SEQ ID NO:54). The alignment was generated using Align X of Vector NTI.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0039] Throughout this application, various publications are referenced. The disclosures of all of these publications and those references cited within those publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains. The terminology used herein is for the purpose of describing specific embodiments only and is not intended to be limiting. As used herein, "a" or "an" can mean one or more, depending upon the context in which it is used. Thus, for example, reference to "a cell" can mean that at least one cell can be used.

[0040] in one embodiment, the invention provides a transgenic plant that overexpresses an isolated polynucleotide identified in Table 1, or a homolog thereof. The transgenic plant of the invention demonstrates an increased tolerance to an environmental stress as compared to a wild type variety of the plant. The overexpression of such isolated nucleic acids in the plant may optionally result in an increase in plant growth or in yield of associated agricultural products, under normal or stress conditions, as compared to a wild type variety of the plant. Without wishing to be bound by any theory, the increased tolerance to an environmental stress, increased growth, and/or increased yield of a transgenic plant of the invention is believed to result from an increase in water use efficiency of the plant.

[0041] As defined herein, a "transgenic plant" is a plant that has been altered using recombinant DNA technology to contain an isolated nucleic acid which would otherwise not be present in the plant. As used herein, the term "plant" includes a whole plant, plant cells, and plant parts. Plant parts include, but are not limited to, stems, roots, ovules, stamens, leaves, embryos, meristematic regions, callus tissue, gametophytes, sporophytes, pollen, microspores, and the like. The transgenic plant of the invention may be male sterile or male fertile, and may further include transgenes other than those that comprise the isolated polynucleotides described herein.

[0042] As used herein, the term "variety" refers to a group of plants within a species that share constant characteristics that separate them from the typical form and from other possible varieties within that species. While possessing at least one distinctive trait, a variety is also characterized by some variation between individuals within the variety, based primarily on the Mendelian segregation of traits among the progeny of succeeding generations. A variety is considered "true breeding" for a particular trait if it is genetically homozygous for that trait to the extent that, when the true-breeding variety is self-pollinated, a significant amount of independent segregation of the trait among the progeny is not observed. In the present invention, the trait arises from the transgenic expression of one or more isolated polynucleotides introduced into a plant variety. As also used herein, the term "wild type variety" refers to a group of plants that are analyzed for comparative purposes as a control plant, wherein the wild type variety plant is identical to the transgenic plant (plant transformed with an isolated polynucleotide in accordance with the invention) with the exception that the wild type variety plant has not been trans-formed to contain an isolated polynucleotide of the invention.

[0043] As defined herein, the term "nucleic acid" and "polynucleotide" are interchangeable and refer to RNA or DNA that is linear or branched, single or double stranded, or a hybrid thereof. The term also encompasses RNA/DNA hybrids. An "isolated" nucleic acid molecule is one that is substantially separated from other nucleic acid molecules which are present in the natural source of the nucleic acid (i.e., sequences encoding other polypeptides). For example, a cloned nucleic acid is considered isolated. A nucleic acid is also considered isolated if it has been altered by human intervention, or placed in a locus or location that is not its natural site, or if it is introduced into a cell by transformation. Moreover, an isolated nucleic acid molecule, such as a cDNA molecule, can be free from some of the other cellular material with which it is naturally associated, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. While it may optionally encompass untranslated sequence located at both the 3' and 5' ends of the coding region of a gene, it may be preferable to remove the sequences which naturally flank the coding region in its naturally occurring replicon.

[0044] As used herein, the term "environmental stress" refers to a sub-optimal condition associated with salinity, drought, nitrogen, temperature, metal, chemical, pathogenic, or oxidative stresses, or any combination thereof. The terms "water use efficiency" and "WUE" refer to the amount of organic matter produced by a plant divided by the amount of water used by the plant in producing it, i.e., the dry weight of a plant in relation to the plant's water use. As used herein, the term "dry weight" refers to everything in the plant other than water, and includes, for example, carbohydrates, proteins, oils, and mineral nutrients.

[0045] Any plant species may be transformed to create a transgenic plant in accordance with the invention. The transgenic plant of the invention may be a dicotyledonous plant or a monocotyledonous plant. For example and without limitation, transgenic plants of the invention may be derived from any of the following diclotyledonous plant families: Leguminosae, including plants such as pea, alfalfa and soybean; Umbelliferae, including plants such as carrot and celery; Solanaceae, including the plants such as tomato, potato, aubergine, tobacco, and pepper; Cruciferae, particularly the genus Brassica, which includes plant such as oilseed rape, beet, cabbage, cauliflower and broccoli); and Arabidopsis thaliana; Compositae, which includes plants such as lettuce; Malvaceae, which includes cotton; Fabaceae, which includes plants such as peanut, and the like. Transgenic plants of the invention may be derived from monocotyledonous plants, such as, for example, wheat, barley, sorghum, millet, rye, triticale, maize, rice, oats, switchgrass, miscanthus, and sugarcane. Transgenic plants of the invention are also embodied as trees such as apple, pear, quince, plum, cherry, peach, nectarine, apricot, papaya, mango, and other woody species including coniferous and deciduous trees such as poplar, pine, sequoia, cedar, oak, willow, and the like. Especially preferred are Arabidopsis thaliana, Nicotiana tabacum, oilseed rape, soybean, corn (maize), wheat, linseed, potato and tagetes.

[0046] As shown in Table 1, one embodiment of the invention is a transgenic plant trans-formed with an expression cassette comprising an isolated polynucleotide encoding a tRNA 2'-phosphotransferase polypeptide. In yeast, the RNA 2'-phosphotransferase Tpt1 protein is an essential protein that catalyzes the final step of tRNA splicing. Although this family of proteins is conserved in eukaryotes, bacteria, and archaea, its function has only been well characterized in yeast. tRNA splicing is conserved in all three major kingdoms, but the mechanisms and enzymes involved differ. These differences leave the exact function of RNA 2'-phosphotransferase proteins in plants unclear, although the enzymatic activity has been demonstrated in tobacco nuclear extracts. All of the RNA 2' phosphotransferase family members, contain a conserved core domain, exemplified by amino acids 98 to 287 of SEQ ID NO:2, and members from Escherichia coil, Arabidopsis thaliana, Schizosaccharomyces pombe, and Homo sapiens are capable of complementing the Saccharomyces cerevisiae tpt1 mutant, indicating similarity of function.

[0047] The transgenic plant of this embodiment may comprise any polynucleotide encoding a tRNA 2'-phosphotransferase. Preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a tRNA 2'-phosphotransferase having a sequence comprising amino acids 98 to 287 of SEQ ID NO:2. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a tRNA 2'-phosphotransferase having a sequence comprising amino acids 1 to 323 of SEQ ID NO:2.

[0048] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a cell division control 2 (CDC2) protein kinase. The CDC2 proteins belong to a specific family of cyclin-dependent kinases (CDKs) in plants commonly referred to as the CDKA family. All of the CDKA proteins contain a highly conserved core kinase domain with a PSTAIRE motif that is the principle site for cyclin interaction to form active CDK-cyclin complexes. An exemplary PSTAIRE motif is represented as amino acids 4 to 287 of SEQ ID NO:4. The CDKA proteins are also subject to posttranslational modification. Phosphorylation of the conserved threonine 14 and tyrosine 15 positions inactivates the CDKA, and phosphorylation of the conserved threonine 161 position activates the CDKA. In yeast these CDKs are involved specifically in G1/S and G2/M controls. In plants, CDKA's are proposed to function in both S and M phase progression and to be involved in cell proliferation and maintenance of cell division competence in differentiating tissues. In Arabidopsis thaliana for example, a mutation of the CDKA1 gene leads to male gametophytic lethality and impairs seed development by reducing seed size.

[0049] The transgenic plant of this embodiment may comprise any polynucleotide encoding a CDC2 protein kinase. Preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a CDKA protein having a sequence comprising amino acids 4 to 287 of SEQ ID NO:4. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a CDKA protein having a sequence comprising amino acids 1 to 294 of SEQ ID NO:4.

[0050] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a leucine-rich repeat (LRR) protein. LRRs are typically found in proteins as 20 to 29 amino acids repeats, each containing an 11 amino acid conserved region with the consensus sequence of LXXLXLXXN/CXL with X as any amino acid and L as valine, leucine, or phenylalanine. The LRR protein of the present invention contains an LRR represented by amino acids 422 to 441 of SEQ ID NO:6. The generally accepted major function of LLRs is to provide a structural scaffold for the formation of protein-protein interactions. LLR-containing proteins are known to be involved in hormone-receptor interactions, enzyme inhibition, cell adhesion, celluar trafficking, plant disease resistance, and bacterial virulence.

[0051] The transgenic plant of this embodiment may comprise any polynucleotide encoding an LRR protein having a sequence comprising amino acids 422 to 441 of SEQ ID NO:6. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a LRR protein having a sequence comprising amino acids 1 to 646 of SEQ ID NO:6.

[0052] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a Ran-binding protein. Ran GTPase (RanGTP) proteins belong to a subfamily of small GTP-binding proteins that are involved in nucleocytoplasmic transport, and are involved in controlling nuclear functions throughout the cell cycle. The Ran binding proteins 1 (RanBP1s) are cytoplasmic proteins that form a complex with the GTP form of RanGTP. The binding domain of RanBP1s that interacts with RanGTP has been identified and is represented by amino acids 51 to 172 of SEQ ID NO:8. The formation of this RanGTP-RanBP1 complex is key to promoting the initial dissociation of RanGTP from transport factors that are exported from the nucleus to the cytoplasm.

[0053] The transgenic plant of this embodiment may comprise any polynucleotide encoding RanBP1 protein having a sequence comprising amino acids 51 to 172 of SEQ ID NO:8. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a RanBP1 protein having a sequence comprising amino acids 1 to 213 of SEQ ID NO:8.

[0054] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a plastid division protein. The FtsZ plastid division proteins are characterized by domains represented by amino acids 139 to 332 of SEQ ID NO:10. The transgenic plant of this embodiment may comprise any polynucleotide encoding a plastid division protein having a sequence comprising amino acids 139 to 332 of SEQ ID NO:10. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a plastid division protein having a sequence comprising amino acids 1 to 490 of SEQ ID NO:10.

[0055] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a mitochondrial substrate carrier protein. The mitochondrial substrate carrier proteins are characterized by domains represented by amino acids 1 to 98 of SEQ ID NO:12. The transgenic plant of this embodiment may comprise any polynucleotide encoding a mitochondrial substrate carrier protein having a sequence comprising amino acids 1 to 98 of SEQ ID NO:12. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a mitochondrial substrate carrier protein having a sequence comprising amino acids 1 to 297 of SEQ ID NO:12.

[0056] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MADS-box protein. The DNA binding and dimerization domains of SRF-type transcription factors comprise MADS-box domains represented by amino acids 9 to 59 of SEQ ID NO:14. The transgenic plant of this embodiment may comprise any polynucleotide encoding an SRF-type transcription factor protein comprising a MADS-box domain having a sequence comprising amino acids 9 to 59 of SEQ ID NO:14. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a MADS-box protein having a sequence comprising amino acids 1 to 187 of SEQ ID NO:14.

[0057] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding an adenosine kinase-1 (ADK-1) protein. The pfkB family of carbohydrate kinases designated ADK-1 comprise domains represented by amino acids 23 to 339 of SEQ ID NO:16. The transgenic plant of this embodiment may comprise any polynucleotide encoding an ADK-1 protein comprising a domain having a sequence comprising amino acids 23 to 339 of SEQ ID NO:16. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding an ADK-1 protein having a sequence comprising amino acids 1 to 343 of SEQ ID NO:16.

[0058] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a zinc finger-6 (ZF-6) protein. These proteins comprise an IBR domain represented by amino acids 210 to 272 of SEQ ID NO:18. The transgenic plant of this embodiment may comprise any polynucleotide encoding a ZF-6 protein comprising a domain having a sequence comprising amino acids 210 to 272 of SEQ ID NO:18. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a ZF-6 protein having a sequence comprising amino acids 1 to 594 of SEQ ID NO:18.

[0059] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a cyclin-dependent kinase regulatory subunit (CDK) protein. These proteins comprise a domain represented by amino acids 1 to 72 of SEQ ID NO:20. The transgenic plant of this embodiment may comprise any polynucleotide encoding a CDK protein comprising a domain having a sequence comprising amino acids 1 to 72 of SEQ ID NO:20. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a CDK protein having a sequence comprising amino acids 1 to 91 of SEQ ID NO:20.

[0060] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a zinc finger-7 (ZF-7) protein. These proteins comprise a C3HC4-type domain represented by amino acids 20 to 60 of SEQ ID NO:22. The transgenic plant of this embodiment may comprise any polynucleotide encoding a ZF-7 protein comprising a domain having a sequence comprising amino acids 20 to 60 of SEQ ID NO:22. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a ZF-7 protein having a sequence comprising amino acids 1 to 347 of SEQ ID NO:22.

[0061] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a MAR-binding protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a MAR-binding protein having a sequence comprising amino acids 1 to 814 of SEQ ID NO:24.

[0062] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a leucine rich repeat receptor protein kinase. The LRP-2 protein of the present invention contains several LRRs, represented by amino acids 111 to 133 of SEQ ID NO:26, amino acids 135 to 158 of SEQ ID NO:26, amino acids 160 to 182 of SEQ ID NO:26, and amino acids 184 to 207 of SEQ ID NO:26. The transgenic plant of this embodiment may comprise any polynucleotide encoding an LRP-2 protein having a sequence comprising amino acids 111 to 133 of SEQ ID NO:26, amino acids 135 to 158 of SEQ ID NO:26, amino acids 160 to 182 of SEQ ID NO:26, and amino acids 184 to 207 of SEQ ID NO:26. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a LRR protein having a sequence comprising amino acids 1 to 251 of SEQ ID NO:26.

[0063] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a phytochrome protein kinase protein. The transgenic plant of this embodiment comprises a polynucleotide encoding a phytochrome protein kinase protein having a sequence comprising amino acids 1 to 689 of SEQ ID NO:28.

[0064] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a synaptobrevin-related protein. These proteins comprise a synaptobrevin domain represented by amino acids 127 to 215 of SEQ ID NO:30. The transgenic plant of this embodiment may comprise any polynucleotide encoding a synaptobrevin-related protein comprising a domain having a sequence comprising amino acids 127 to 215 of SEQ ID NO:30. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a synaptobrevin-related protein having a sequence comprising amino acids 1 to 222 of SEQ ID NO:30.

[0065] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a calcineurin B protein. In plants, a family of proteins has been found that are calcium sensor proteins with similarity to both the regulatory B subunit of calcineurin and neuronal calcium sensors of animals. These proteins have been termed calcineurin B-like proteins (CBL). These CBL proteins contain EF hand motifs that are structurally important for calcium binding and interact specifically with a group of Ser/Thr protein kinases, designated as CBL-Interacting protein kinases (CIPK). CIPKs most likely represent targets of calcium sensed and transduced by CBL proteins.

[0066] Each EF hand consists of a loop of 12 amino acids flanked by two alpha helices, which binds a single calcium ion via the loop domain. These proteins have also been found to bind magnesium ions. Proteins with four EF hand motifs usually have two structural domains, each formed by a pair of EF hand motifs separated by a flexible linker. Binding of the metal ion to the EF hand protein leads to a conformational change that exposes a hydrophobic surface, which binds to a target sequence. Many EF hand containing proteins also contain a myristoylation site at the N-terminus, with consensus sequence of MGXXXS/T, with X representing any amino acid. Myristoylation at this site promotes protein-protein or protein membrane interaction. This myristoylation site is not present in the EST321 (SEQ ID NO:32) sequence, potentially indicating that the EST321 protein could belong to a different class of EF hand domain containing proteins.

[0067] The calcineurin B subunit protein of the present invention contains several EF hand motifs, represented by amino acids 37 to 65 of SEQ ID NO:32, amino acids 106 to 134 of SEQ ID NO:32, and amino acids 142 to 170 of SEQ ID NO:32. The transgenic plant of this embodiment may comprise any polynucleotide encoding a calcineurin B subunit protein having a sequence comprising amino acids 37 to 65 of SEQ ID NO:32, amino acids 106 to 134 of SEQ ID NO:32, and amino acids 142 to 170 of SEQ ID NO:32. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a calcineurin B subunit protein having a sequence comprising amino acids 1 to 182 of SEQ ID NO:32.

[0068] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a caleosin-related protein. Caleosins are a family of proteins are presumably modulated by calcium-binding and phosphorylation state and are thought to be involved in fusion of membranes and oil bodies. These proteins contain several domains, an N-terminal region with a single calcium ion binding EF-hand motif, a central hydrophobic region with a potential membrane anchor, and a C-terminal region with conserved protein kinase phosphorylation sites. The presence of only a single EF hand motif is unusual for most EF hand containing proteins. It has been postulated that this single EF hand domain may interact with the membrane surface or another protein in order to form the coordinated double EF hand domain interaction found in most other EF hand proteins.

[0069] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a caleosin-related protein. These proteins comprise a caleosin domain represented by amino acids 26 to 229 of SEQ ID NO:34. The transgenic plant of this embodiment may comprise any polynucleotide encoding a caleosin-related protein comprising a domain having a sequence comprising amino acids 26 to 229 of SEQ ID NO:34. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a caleosin-related protein having a sequence comprising amino acids 1 to 239 of SEQ ID NO:34.

[0070] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a histone deacetylase protein. Nucleosomes consist of histones and DNA, which are essential for packaging DNA into chromosomes. Lysine at the N-terminal ends of core histones are the predominant sites for acetylation and methylation, and histone deacetylases catalyze the removal of the acetyl group from these lysine side chains. Active genes are preferentially associated with highly acetylated histones and inactive genes are associated with hypoacetylated histones. Acetylation results in charge neutralization of histones and weakens histone/DNA contacts. In plants, histone hyperacetylation is correlated with gene activity.

[0071] Histones are found to be associated with large multisubunit complexes. Three distinct families of histone deacytelases are found in plants, the RPD3/HDA family, SIR2 family, and the plant specific HD2 family. The RPD3/HDA1 family is found in all eukaryotic organisms, and members possess a complete histone deacetylase domain. Some histone deacetylase proteins possess unique regions outside the histone deacetylase domain that may be important for function and/or specificity of these proteins.

[0072] The histone deacetylases of the present invention are characterized by the following domains: from amino acids 6 to 318 of SEQ ID NO:36; from amino acids 6 to 318 of SEQ ID NO:38; from amino acids 20 to 332 of SEQ ID NO:40; from amino acids 8 to 322 of SEQ ID NO:42; from amino acids 6 to 318 of SEQ ID NO:44; from amino acids 23 to 333 of SEQ ID NO:46; from amino acids 8 to 321 of SEQ ID NO:48; from amino acids 6 to 318 of SEQ ID NO:50; from amino acids 56 to 382 of SEQ ID NO:52; and from amino acids 23 to 333 of SEQ ID NO:54.

[0073] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an polynucleotide encoding a histone deacetylase protein. The transgenic plant of this embodiment may comprise any polynucleotide encoding a histone deacetylase protein comprising a domain having a sequence selected from the group consisting of amino acids 6 to 318 of SEQ ID NO:36; amino acids 6 to 318 of SEQ ID NO:38; amino acids 20 to 332 of SEQ ID NO:40; amino adds 8 to 322 of SEQ ID NO:42; amino acids 6 to 318 of SEQ ID NO:44; amino acids 23 to 333 of SEQ ID NO:46; amino acids 8 to 321 of SEQ ID NO:48; amino acids 6 to 318 of SEQ ID NO:50; amino acids 56 to 382 of SEQ ID NO:52; and amino acids 23 to 333 of SEQ ID NO:54. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a histone deacetylase protein selected from the group consisting of a protein having a sequence comprising amino acids 1 to 431 of SEQ ID NO:36; a protein having a sequence comprising amino acids 1 to 426 of SEQ ID NO:38; a protein having a sequence comprising amino acids 1 to 470 of SEQ ID NO:40; a protein having a sequence comprising amino acids 1 to 363 of SEQ ID NO:42; a protein having a sequence comprising amino acids 1 to 429 of SEQ ID NO:44; a protein having a sequence comprising amino acids 1 to 518 of SEQ ID NO:46; a protein having a sequence comprising amino acids 1 to 334 of SEQ ID NO:48; a protein having a sequence comprising amino acids 1 to 429 of SEQ ID NO:50; a protein having a sequence comprising amino acids 1 to 417 of SEQ ID NO:52; and a protein having a sequence comprising amino acids 1 to 519 of SEQ ID NO:54.

[0074] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a leucine-rich repeat (LRR) protein. LRRs are typically found in proteins as 20 to 29 amino acids repeats, each containing an 11 amino acid conserved region with the consensus sequence of LXXLXLXXN/CXL with X as any amino acid and L as valine, leucine, or phenylalanine. The LRR protein of the present invention contains an LRR represented by amino acids 422 to 441 of SEQ ID NO:56. The generally accepted major function of LLRs is to provide a structural scaffold for the formation of protein-protein interactions. LLR-containing proteins are known to be involved in hormone-receptor interactions, enzyme inhibition, cell adhesion, celluar trafficking, plant disease resistance, and bacterial virulence.

[0075] The transgenic plant of this embodiment may comprise any polynucleotide encoding an LRR protein having a sequence comprising amino acids 422 to 441 of SEQ ID NO:56. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a LRR protein having a sequence comprising amino acids 1 to 698 of SEQ ID NO:56.

[0076] In another embodiment, the invention provides a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a leucine-rich repeat (LRR) protein. LRRs are typically found in proteins as 20 to 29 amino acids repeats, each containing an 11 amino acid conserved region with the consensus sequence of LXXLXLXXN/CXL with X as any amino acid and L as valine, leucine, or phenylalanine. The LRR protein of the present invention contains an LRR represented by amino acids 422 to 441 of SEQ ID NO:58. The generally accepted major function of LLRs is to provide a structural scaffold for the formation of protein-protein interactions. LLR-containing proteins are known to be involved in hormone-receptor interactions, enzyme inhibition, cell adhesion, celluar trafficking, plant disease resistance, and bacterial virulence.

[0077] The transgenic plant of this embodiment may comprise any polynucleotide encoding an LRR protein having a sequence comprising amino acids 422 to 441 of SEQ ID NO:58. More preferably, the transgenic plant of this embodiment comprises a polynucleotide encoding a LRR protein having a sequence comprising amino acids 1 to 665 of SEQ ID NO:58.

[0078] The invention further provides a seed produced by a transgenic plant expressing polynucleotide listed in Table 1, wherein the seed contains the polynucleotide, and wherein the plant is true breeding for increased growth and/or yield under normal and/or stress conditions and/or increased tolerance to an environmental stress as compared to a wild type variety of the plant. The invention also provides a product produced by or from the transgenic plants expressing the polynucleotide, their plant parts, or their seeds. The product can be obtained using various methods well known in the art. As used herein, the word "product" includes, but not limited to, a foodstuff, feedstuff, a food supplement, feed supplement, cosmetic or pharmaceutical. Foodstuffs are regarded as compositions used for nutrition or for supplementing nutrition. Animal feedstuffs and animal feed supplements, in particular, are regarded as foodstuffs. The invention further provides an agricultural product produced by any of the transgenic plants, plant parts, and plant seeds. Agricultural products include, but are not limited to, plant extracts, proteins, amino acids, carbohydrates, fats, oils, polymers, vitamins, and the like.

[0079] In a preferred embodiment, an isolated polynucleotide of the invention comprises a polynucleotide having a sequence selected from the group consisting of the polynucleotide sequences listed in Table 1. These polynucleotides may comprise sequences of the coding region, as well as 5' untranslated sequences and 3' untranslated sequences. Table 2 describes potential start and end positions of the coding regions of the P. patens polynucleotides of the invention, and alternative open reading frames that may be present in the sense or antisense strands of these polynucleotides. Alternatively, the polynucleotides of the invention can comprise only the coding region of the nucleotide sequences listed in Table 1, as indicated in Table 2, or the polynucleotides can contain whole genomic fragments isolated from genomic DNA. Thus the invention is also embodied as an isolated polynucleotide having a sequence selected from the group consisting of the sequences listed in Table 1 or Table 2.

TABLE-US-00002 TABLE 2 SEQ Start End Gene GENE ID Orf Posi- Posi- Name ID NO ORFs Number Strand tion tion PpTPT-1 EST 214 1 1 1 sense 113 1104 PpCDC2-1 EST 280 3 2 1 sense 37 921 PpCDC2-1 EST 280 3 2 2 anti- 380 42 sense PpLRP-1 EST 298a 5 1 1 sense 144 2084 EST 298b 55 1 1 sense 143 2236 EST 298c 57 1 1 sense 1 1998 PpRBP-1 EST 300 7 1 1 sense 55 696 PpPD-1 EST 362 9 2 1 sense 47 1519 PpPD-1 EST 362 9 2 2 anti- 1197 604 sense PpMSC-1 EST 378 11 2 1 sense 453 1346 PpMSC-1 EST 378 11 2 2 anti- 1314 1027 sense PpMBP-1 EST 398 13 1 1 sense 33 878 PpAK-1 EST 407 15 3 1 sense 25 1056 PpAK-1 EST 407 15 3 2 sense 381 632 PpAK-1 EST 407 15 3 3 anti- 506 270 sense PpZF-6 EST 458 17 1 1 sense 126 1910 PpCDK-1 EST 479 19 2 1 sense 248 523 PpCDK-1 EST 479 19 2 2 anti- 304 104 sense PpZF-7 EST 520 21 2 1 sense 276 1319 PpZF-7 EST 520 21 2 2 sense 583 813 PpMFP-1 EST 544 23 1 1 sense 127 2571 PpLRP-2 EST 545 25 3 1 sense 225 980 PpLRP-2 EST 545 25 3 2 sense 416 694 PpLRP-2 EST 545 25 3 3 anti- 469 167 sense PpPPK-1 EST 549 27 1 1 sense 145 2214 PpSRP-1 EST 554 29 1 1 sense 20 688 PpCBL-1 EST 321 31 2 1 sense 43 591 PpCBL-1 EST 321 31 2 2 sense 803 1171 PpCBL-2 EST 416 33 1 1 sense 16 735 PpHD-1 EST 468 35 2 1 sense 166 1461 PpHD-1 EST 468 35 2 2 anti- 420 175 sense

[0080] A polynucleotide of the invention can be isolated using standard molecular biology techniques and the sequence information provided herein. For example, P. patens cDNAs of the invention were isolated from a P. patens library using a portion of the sequence disclosed herein. Synthetic oligonucleotide primers for polymerase chain reaction amplification can be designed based upon the nucleotide sequence shown in Table 1. A nucleic acid molecule of the invention can be amplified using cDNA or, alternatively, genomic DNA, as a template and appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid molecule so amplified can be cloned into an appropriate vector and characterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding to the nucleotide sequences listed in Table 1 can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.

[0081] "Homologs" are defined herein as two nucleic acids or polypeptides that have similar, or substantially identical, nucleotide or amino acid sequences, respectively. Homologs include allelic variants, analogs, and orthologs, as defined below. As used herein, the term "analogs" refers to two nucleic acids that have the same or similar function, but that have evolved separately in unrelated organisms. As used herein, the term "orthologs" refers to two nucleic acids from different species, but that have evolved from a common ancestral gene by speclation. The term homolog further encompasses nucleic acid molecules that differ from one of the nucleotide sequences shown in Table 1 due to degeneracy of the genetic code and thus encode the same polypeptide. As used herein, a "naturally occurring" nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature (e.g., encodes a natural polypeptide).

[0082] To determine the percent sequence identity of two amino acid sequences (e.g., one of the polypeptide sequences of Table 1 and a homolog thereof), the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of one polypeptide for optimal alignment with the other polypeptide or nucleic acid). The amino acid residues at corresponding amino acid positions are then compared. When a position in one sequence is occupied by the same amino acid residue as the corresponding position in the other sequence then the molecules are identical at that position. The same type of comparison can be made between two nucleic acid sequences.

[0083] Preferably, the isolated amino acid homologs, analogs, and orthologs of the polypeptides of the present invention are at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-75%, 75-80%, 80-85%, 85-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99%, or more identical to an entire amino acid sequence identified in Table 1. In another preferred embodiment, an isolated nucleic acid homolog of the invention comprises a nucleotide sequence which is at least about 40-60%, preferably at least about 60-70%, more preferably at least about 70-75%, 75-80%, 80-85%, 85-90%, or 90-95%, and even more preferably at least about 95%, 96%, 97%, 98%, 99%, or more identical to a nucleotide sequence shown in Table 1 or Table 2.

[0084] For the purposes of the invention, the percent sequence identity between two nucleic acid or polypeptide sequences is determined using the Vector NTI 9.0 (PC) software package (Invitrogen, 1600 Faraday Ave., Carlsbad, Calif. 92008). A gap opening penalty of 15 and a gap extension penalty of 6.66 are used for determining the percent identity of two nucleic acids. A gap opening penalty of 10 and a gap extension penalty of 0.1 are used for determining the percent identity of two polypeptides. All other parameters are set at the default settings. For purposes of a multiple alignment (Clustal W algorithm), the gap opening penalty is 10, and the gap extension penalty is 0.05 with blosum62 matrix. It is to be understood that for the purposes of determining sequence identity when comparing a DNA sequence to an RNA sequence, a thymidine nucleotide is equivalent to a uracil nucleotide.

[0085] Nucleic acid molecules corresponding to homologs, analogs, and orthologs of the polypeptides listed in Table 1 can be isolated based on their identity to said polypeptides, using the polynucleotides encoding the respective polypeptides or primers based thereon, as hybridization probes according to standard hybridization techniques under stringent hybridization conditions. As used herein with regard to hybridization for DNA to a DNA blot, the term "stringent conditions" refers to hybridization overnight at 60° C. in 10× Denhart's solution, 6×SSC, 0.5% SDS, and 100 μg/ml denatured salmon sperm DNA. Blots are washed sequentially at 62° C. for 30 minutes each time in 3×SSC/0.1% SDS, followed by 1×SSC/0.1% SDS, and finally 0.1×SSC/0.1% SDS. As also used herein, in a preferred embodiment, the phrase "stringent conditions" refers to hybridization in a 6×SSC solution at 65° C. in another embodiment, "highly stringent conditions" refers to hybridization overnight at 65° C. in 10× Denhart's solution, 6×SSC, 0.5% SDS and 100 μg/ml denatured salmon sperm DNA. Blots are washed sequentially at 65° C. for 30 minutes each time in 3×SSC/0.1% SDS, followed by 1×SSC/0.1% SDS, and finally 0.1×SSC/0.1% SDS. Methods for nucleic acid hybridizations are described in Meinkoth and Wahl, 1984, Anal. Biochem. 138:267-284; well known in the art (see, for example, Current Protocols in Molecular Biology, Chapter 2, Ausubel et al., eds., Greene Publishing and Wiley-Interscience, New York, 1995; and Tijssen, 1993, Laboratory Techniques in Biochemistry and Molecular Biology: Hybridization with Nucleic Acid Probes, Part I, Chapter 2, Elsevier, New York, 1993). Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent or highly stringent conditions to a nucleotide sequence listed in Table 1 corresponds to a naturally occurring nucleic acid molecule.

[0086] There are a variety of methods that can be used to produce libraries of potential homologs from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene is then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, In one mixture, of all of the sequences encoding the desired set of potential sequences. Methods for synthesizing degenerate oligonucleotides are known in the art (See, e.g., Narang, 1983, Tetrahedron 39:3; Itakura et al., 1984, Annu. Rev. Biochem. 53:323; Itakura et al., 1984, Science 198:1056; Ike et al., 1983, Nucleic Acid Res. 11:477).

[0087] Additionally, optimized nucleic acids can be created. Preferably, an optimized nucleic acid encodes a polypeptide that has a function similar to those of the polypeptides listed in Table 1 and/or modulates a plant's growth and/or yield under normal and/or water-limited conditions and/or tolerance to an environmental stress, and more preferably increases a plant's growth and/or yield under normal and/or water-limited conditions and/or tolerance to an environmental stress upon its overexpression in the plant. As used herein, "optimized" refers to a nucleic acid that is genetically engineered to increase its expression in a given plant or animal. To provide plant optimized nucleic acids, the DNA sequence of the gene can be modified to: 1) comprise codons preferred by highly expressed plant genes; 2) comprise an A+T content in nucleotide base composition to that substantially found in plants; 3) form a plant initiation sequence; 4) to eliminate sequences that cause destabilization, inappropriate polyadenylation, degradation and termination of RNA, or that form secondary structure hairpins or RNA splice sites; or 5) elimination of antisense open reading frames. Increased expression of nucleic acids in plants can be achieved by utilizing the distribution frequency of codon usage in plants in general or in a particular plant. Methods for optimizing nucleic acid expression in plants can be found in EPA 0359472; EPA 0385962; PCT Application No. WO 91/16432; U.S. Pat. No. 5,380,831; U.S. Pat. No. 5,436,391; Perlack et al., 1991, Proc. Natl. Acad. Sci. USA 88:3324-3328; and Murray et al., 1989, Nucleic Acids Res. 17:477-498.

[0088] An isolated polynucleotide of the invention can be optimized such that its distribution frequency of codon usage deviates, preferably, no more than 25% from that of highly expressed plant genes and, more preferably, no more than about 10%. In addition, consideration is given to the percentage G+C content of the degenerate third base (monocotyledons appear to favor G+C in this position, whereas dicotyledons do not). It is also recognized that the XCG (where X is A, T, C, or G) nucleotide is the least preferred codon in dicots, whereas the XTA codon is avoided in both monocots and dicots. Optimized nucleic acids of this invention also preferably have CG and TA doublet avoidance indices closely approximating those of the chosen host plant. More preferably, these indices deviate from that of the host by no more than about 10-15%.

[0089] The invention further provides an isolated recombinant expression vector comprising a polynucleotide as described above, wherein expression of the vector in a host cell results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to environmental stress as compared to a wild type variety of the host cell. The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed. As used herein with respect to a recombinant expression vector, "operatively linked" is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence (e.g., in a bacterial or plant host cell when the vector is introduced into the host cell). The term "regulatory sequence" is intended to include promoters, enhancers, and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are well known in the art. Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cells and those that direct expression of the nucleotide sequence only in certain host cells or under certain conditions. It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of polypeptide desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce polypeptides encoded by nucleic acids as described herein.

[0090] Plant gene expression should be operatively linked to an appropriate promoter conferring gene expression in a timely, cell specific, or tissue specific manner. Promoters useful in the expression cassettes of the invention include any promoter that is capable of initiating transcription in a plant cell. Such promoters include, but are not limited to, those that can be obtained from plants, plant viruses, and bacteria that contain genes that are expressed in plants, such as Agrobacterium and Rhizobium.

[0091] The promoter may be constitutive, inducible, developmental stage-preferred, cell type-preferred, tissue-preferred, or organ-preferred. Constitutive promoters are active under most conditions. Examples of constitutive promoters include the CaMV 19S and 35S promoters (Odell et al., 1985, Nature 313:810-812), the sX CaMV 35S promoter (Kay et al., 1987, Science 236:1299-1302) the Sep1 promoter, the rice actin promoter (McElroy et al., 1990, Plant Cell 2:163-171), the Arabidopsis actin promoter, the ubiquitan promoter (Christensen et al., 1989, Plant Molec. Biol. 18:676-689), pEmu (Last et al., 1991, Theor. Appl. Genet. 81:581-588), the figwort mosaic virus 35S promoter, the Smas promoter (Velten et al., 1984, EMBO J 3:2723-2730), the super promoter (U.S. Pat. No. 5,955,646), the GRP1-8 promoter, the cinnamyl alcohol dehydrogenase promoter (U.S. Pat. No. 5,683,439), promoters from the T-DNA of Agrobacterium, such as mannopine synthase, nopaline synthase, and octopine synthase, the small subunit of ribulose biphosphate carboxylase (ssuRUBISCO) promoter, and the like.

[0092] Inducible promoters are preferentially active under certain environmental conditions, such as the presence or absence of a nutrient or metabolite, heat or cold, light, pathogen attack, anaerobic conditions, and the like. For example, the hsp80 promoter from Brassica is induced by heat shock; the PPDK promoter is induced by light; the PR-1 promoters from tobacco, Arabidopsis, and maize are inducible by infection with a pathogen; and the Adh1 promoter is induced by hypoxia and cold stress. Plant gene expression can also be facilitated via an inducible promoter (For a review, see Gatz, 1997, Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:89-108). Chemically inducible promoters are especially suitable if gene expression is wanted to occur in a time specific manner. Examples of such promoters are a salicylic acid inducible promoter (PCT Application No. WO 95/19443), a tetracycline inducible promoter (Gatz et al., 1992, Plant J. 2: 397-404), and an ethanol inducible promoter (PCT Application No. WO 93/21334).

[0093] In one preferred embodiment of the present invention, the inducible promoter is a stress-inducible promoter. For the purposes of the invention, stress-inducible promoters are preferentially active under one or more of the following stresses: sub-optimal conditions associated with salinity, drought, nitrogen, temperature, metal, chemical, pathogenic, and oxidative stresses. Stress inducible promoters include, but are not limited to, Cor78 (Chak et al., 2000, Planta 210:875-883; Hovath et al., 1993, Plant Physiol. 103:1047-1053), Cor15a (Artus et al., 1996, PNAS 93(23):13404-09), Rci2A (Medina et al., 2001, Plant Physiol. 125:1655-66; Nylander et al., 2001, Plant Mol. Biol. 45:341-52; Navarre and Goffeau, 2000, EMBO J. 19:2515-24; Capel et al., 1997, Plant Physiol. 115:569-76), Rd22 (Xiong et al., 2001, Plant Cell 13:2063-83; Abe et al., 1997, Plant Cell 9:1859-68; Iwasaki et al., 1995, Mol. Gen. Genet. 247:391-8), cDet6 (Lang and Palve, 1992, Plant Mol. Biol. 20:951-62), ADH1 (Hoeren et al., 1998, Genetics 149:479-90), KAT1 (Nakamura et al., 1995, Plant Physiol. 109:371-4), KST1 (Muller-Rober et al., 1995, EMBO 14:2409-16), Rha1 (Terryn et al., 1993, Plant Cell 5:1761-9; Terryn et al., 1992, FEBS Lett. 299(3):287-90), ARSK1 (Atkinson et al., 1997, GenBank Accession # L22302, and PCT Application No. WO 97/20057), PtxA (Plesch et al., GenBank Accession # X67427), SbHRGP3 (Ahn et al., 1996, Plant Cell 8:1477-90), GH3 (Liu et al., 1994, Plant Cell 6:645-57), the pathogen inducible PRP1-gene promoter (Ward et al., 1993, Plant. Mol. Biol. 22:361-366), the heat inducible hsp80-promoter from tomato (U.S. Pat. No. 5,187,267), cold inducible alpha-amylase promoter from potato (PCT Application No. WO 96/12814), or the wound-inducible pinII-promoter (European Patent No. 375091). For other examples of drought, cold, and salt-inducible promoters, such as the RD29A promoter, see Yamaguchi-Shinozalei et al., 1993, Mol. Gen. Genet. 236:331-340.

[0094] Developmental stage-preferred promoters are preferentially expressed at certain stages of development. Tissue and organ preferred promoters include those that are preferentially expressed in certain tissues or organs, such as leaves, roots, seeds, or xylem. Examples of tissue-preferred and organ-preferred promoters include, but are not limited to fruit-preferred, ovule-preferred, male tissue-preferred, seed-preferred, integument-preferred, tuber-preferred, stalk-preferred, pericarp-preferred, leaf-preferred, stigma-preferred, pollen-preferred, anther-preferred, petal-preferred, sepal-preferred, pedicel-preferred, silique-preferred, stem-preferred, root-preferred promoters, and the like. Seed-preferred promoters are preferentially expressed during seed development and/or germination. For example, seed-preferred promoters can be embryo-preferred, endosperm-preferred, and seed coat-preferred (See Thompson et al., 1989, BioEssays 10:108). Examples of seed-preferred promoters include, but are not limited to, cellulose synthase (celA), Cim1, gamma-zein, globulin-1, maize 19 kD zein (cZ19B1), and the like.

[0095] Other suitable tissue-preferred or organ-preferred promoters include the napin-gene promoter from rapeseed (U.S. Pat. No. 5,608,152), the USP-promoter from Vicia faba (Baeumlein et al., 1991, Mol. Gen. Genet. 225(3): 459-67), the oleosin-promoter from Arabidopsis (PCT Application No. WO 98/45461), the phaseolin-promoter from Phaseolus vulgaris (U.S. Pat. No. 5,504,200), the Bce4-promoter from Brassica (PCT Application No. WO 91/13980), or the legumin B4 promoter (LeB4; Baeumlein et al., 1992, Plant Journal, 2(2): 233-9), as well as promoters conferring seed specific expression in monocot plants like maize, barley, wheat, rye, rice, etc. Suitable promoters to note are the Ipt2 or Ipt1-gene promoter from barley (PCT Application No. WO 95/15389 and PCT Application No. WO 95/23230) or those described in PCT Application No. WO 99/16890 (promoters from the barley hordein-gene, rice glutelin gene, rice oryzin gene, rice prolamin gene, wheat gliadin gene, wheat glutelin gene, oat glutelin gene, Sorghum kasirin-gene, and rye secalin gene).

[0096] Other promoters useful in the expression cassettes of the invention include, but are not limited to, the major chlorophyll a/b binding protein promoter, histone promoters, the Ap3 promoter, the β-conglycin promoter, the napin promoter, the soybean lectin promoter, the maize 15 kD zein promoter, the 22 kD zein promoter, the 27 kD zein promoter, the g-zein promoter, the waxy, shrunken 1, shrunken 2, and bronze promoters, the Zm13 promoter (U.S. Pat. No. 5,086,169), the maize polygalacturonase promoters (PG) (U.S. Pat. Nos. 5,412,085 and 5,545,546), and the SUBS promoter (U.S. Pat. No. 5,470,359), as well as synthetic or other natural promoters.

[0097] Additional flexibility in controlling heterologous gene expression in plants may be obtained by using DNA binding domains and response elements from heterologous sources (i.e., DNA binding domains from non-plant sources). An example of such a heterologous DNA binding domain is the LexA DNA binding domain (Brent and Ptashne, 1985, Cell 43:729-736).

[0098] In a preferred embodiment of the present invention, the polynucleotides listed in Table 1 are expressed in plant cells from higher plants (e.g., the spermatophytes, such as crop plants). A polynucleotide may be "introduced" into a plant cell by any means, including transfection, transformation or transduction, electroporation, particle bombardment, agroinfection, and the like. Suitable methods for transforming or transfecting plant cells are disclosed, for example, using particle bombardment as set forth in U.S. Pat. Nos. 4,945,050; 5,036,006; 5,100,792; 5,302,523; 5,464,765; 5,120,657; 6,084,154; and the like. More preferably, the transgenic corn seed of the invention may be made using Agrobacterium transformation, as described in U.S. Pat. Nos. 5,591,616; 5,731,179; 5,981,840; 5,990,387; 6,162,965; 6,420,630, U.S. patent application publication number 2002/0104132, and the like. Transformation of soybean can be performed using for example a technique described in European Patent No. EP 0424047, U.S. Pat. No. 5,322,783, European Patent No. EP 0397 687, U.S. Pat. No. 5,376,543, or U.S. Pat. No. 5,169,770. A specific example of wheat transformation can be found in PCT Application No. WO 93/07256. Cotton may be transformed using methods disclosed in U.S. Pat. Nos. 5,004,863; 5,159,135; 5,846,797, and the like. Rice may be transformed using methods disclosed in U.S. Pat. Nos. 4,666,844; 5,350,688; 6,153,813; 6,333,449; 6,288,312; 6,365,807; 6,329,571, and the like. Other plant transformation methods are disclosed, for example, in U.S. Pat. Nos. 5,932,782; 6,153,811; 6,140,553; 5,969,213; 6,020,539, and the like. Any plant transformation method suitable for inserting a transgene into a particular plant may be used in accordance with the invention.

[0099] According to the present invention, the introduced polynucleotide may be maintained in the plant cell stably if it is incorporated into a non-chromosomal autonomous replicon or integrated into the plant chromosomes. Alternatively, the introduced polynucleotide may be present on an extra-chromosomal non-replicating vector and may be transiently expressed or transiently active.

[0100] Another aspect of the invention pertains to an isolated polypeptide having a sequence selected from the group consisting of the polypeptide sequences listed in Table 1. An "isolated" or "purified" polypeptide is free of some of the cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. The language "substantially free of cellular material" includes preparations of a polypeptide in which the polypeptide is separated from some of the cellular components of the cells in which it is naturally or recombinantly produced. In one embodiment, the language "substantially free of cellular material" includes preparations of a polypeptide of the invention having less than about 30% (by dry weight) of contaminating polypeptides, more preferably less than about 20% of contaminating polypeptides, still more preferably less than about 10% of contaminating polypeptides, and most preferably less than about 5% contaminating polypeptides.

[0101] The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one skilled in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, applications and examples for the determination of many enzyme activities are abundant and well known to one skilled in the art.

[0102] The invention is also embodied in a method of producing a transgenic plant comprising at least one polynucleotide listed in Table 1 or Table 2, wherein expression of the polynucleotide in the plant results in the plant's increased growth and/or yield under normal and/or water-limited conditions and/or increased tolerance to an environmental stress as compared to a wild type variety of the plant comprising the steps of: (a) introducing into a plant cell an expression vector comprising at least one polynucleotide listed in Table 1 or Table 2, and (b) generating from the plant cell a transgenic plant that expresses the polynucleotide, wherein expression of the polynucleotide in the transgenic plant results in the plant's increased growth and/or yield under normal or water-limited conditions and/or increased tolerance to environmental stress as compared to a wild type variety of the plant. The plant cell may be, but is not limited to, a protoplast, gamete producing cell, and a cell that regenerates into a whole plant. As used herein, the term "transgenic" refers to any plant, plant cell, callus, plant tissue, or plant part, that contains at least one recombinant polynucleotide listed in Table 1 or Table 2. In many cases, the recombinant polynucleotide is stably integrated into a chromosome or stable extra-chromosomal element, so that it is passed on to successive generations.

[0103] The present invention also provides a method of increasing a plant's growth and/or yield under normal and/or water-limited conditions and/or increasing a plant's tolerance to an environmental stress comprising the steps of increasing the expression of at least one polynucleotide listed in Table 1 or Table 2 in the plant. Expression of a polynucleotide listed in Table 1 or Table 2 can be increased by any method known to those of skill in the art.

[0104] The effect of the genetic modification on plant growth and/or yield and/or stress tolerance can be assessed by growing the modified plant under normal and/or less than suitable conditions and then analyzing the growth characteristics and/or metabolism of the plant. Such analysis techniques are well known to one skilled in the art, and include dry weight, wet weight, polypeptide synthesis, carbohydrate synthesis, lipid synthesis, evapotranspiration rates, general plant and/or crop yield, flowering, reproduction, seed setting, root growth, respiration rates, photosynthesis rates, metabolite composition, etc., using methods known to those of skill in biotechnology.

[0105] The invention is further illustrated by the following examples, which are not to be construed in any way as imposing limitations upon the scope thereof.

Example 1

Identification of P. patens Open Reading Frames

[0106] cDNA libraries made from plants of the species P. patens (Hedw.) B.S.G. from the collection of the genetic studies section of the University of Hamburg were sequences using standard methods. The plants originated from the strain 16/14 collected by H. L. K. Whitehouse in Gransden Wood, Huntingdonshire (England), which was subcultured from a spore by Engel (1968, Am. J. Bot. 55:438-446).

[0107] Sequences were processed and annotated using the software package EST-MAX commercially provided by Bio-Max (Munich, Germany). The program incorporates practically alt bioinformatics methods important for functional and structural characterization of protein sequences. For reference, see the website at pedant.mips.biochem.mpg.de. The most important algorithms incorporated in EST-MAX are: FASTA (Very sensitive sequence database searches with estimates of statistical significance; Pearson, 1990, Methods Enzymol. 183:63-98); BLAST (Very sensitive sequence database searches with estimates of statistical significance; Altschul et al., 1990, Journal of Molecular Biology 215:403-10); PREDATOR (High-accuracy secondary structure prediction from single and multiple sequences; Frishman and Argos, 1997, Proteins 27:329-335); CLUSTALW (Multiple sequence alignment; Thompson et al., 1994, Nucleic Acids Research 22:4673-4680); TMAP (Transmembrane region prediction from multiply aligned sequences; Persson and Argos, 1994, J. Mol. Biol. 237:182-192); ALOM2 (Transmembrane region prediction from single sequences; Klein et al., 1984, Biochim. Biophys. Acta 787:221-226. Version 2 by Dr. K. Nakai); PROSEARCH (Detection of PROSITE protein sequence patterns; Kolakowski et al., 1992, Biotechniques 13, 919-921); BLIMPS (Similarity searches against a database of ungapped blocks, Wallace and Henikoff, 1992, Comput Appl Biosci. 8(3):249-54); PATMAT (a searching and extraction program for sequence, pattern and block queries and databases, CABIOS 8:249-254. Written by Bill Alford).

[0108] P. patens partial cDNAs (ESTs) were identified in the P. patens EST sequencing program using the program EST-MAX through BLAST analysis. The full-length nucleotide cDNA sequences were determined using known methods. The identity and similarity of the amino acid sequences of the disclosed polypeptide sequences to known protein sequences are shown in Tables 2-19 (Pairwise Comparison was used: gap penalty: 10; gap extension penalty: 0.1; score matrix: blosum 62).

TABLE-US-00003 TABLE 3 Comparison of PpTPT-1 (SEQ ID NO: 2) to known RNA 2' phosphotransferases Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) XP_550615 O. sativa 45.4 56.2 NP_197750 A. thaliana 34.1 43.0 NP_182058 A. thaliana 39.6 48.9 NP_594515 Schizosaccharomyces 21.3 29.1 pombe NP_788477 Drosophila 25.5 34.7 melanogaster

TABLE-US-00004 TABLE 4 Comparison of PpCDC2-1 (SEQ ID NO: 4) to known Cell Division Control Protein 2 Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) S42049 Picea abies 92.9 96.3 Q40790 Pinus contorta 92.5 96.3 CDC2_CHERU Chenopodium 91.5 95.9 rubrum Q9AUH4 Populus tremula × 90.5 95.9 P. tremuloides Q8W2D3 Helianthus annuus 89.5 95.2

TABLE-US-00005 TABLE 5 Comparison of PpLRP-1 (SEQ ID NO: 6) to known Leucine Rich Repeat Family Proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) NP_912570 O. sativa 27.6 42.1 NP_921136 O. sativa 27.2 40.3 AAF71805 A. thaliana 24.4 33.6 NP_177947 A. thaliana 28.1 38.5 G96811 A. thaliana 28.8 40.3

TABLE-US-00006 TABLE 6 Comparison of PpRBP-1 (SEQ ID NO: 8) to known Ran binding protein 1 Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) Q94K24 Lycopersicon 50.7 63.3 esculentum Q9LUZ8 A. thaliana 39.4 51.9 NP_200667 A. thaliana 44.3 58.3 O04149 A. thaliana 44.1 55.1 NP_172194 A thaliana 44.4 55.1

TABLE-US-00007 TABLE 7 Comparison of PpPD-1 (SEQ ID NO: 10) to known Plastid division ftsZ proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) Q70ZZ6 P. patens 100 100 Q75ZR3 Nannochloris 42.6 53.0 bacillaris ZP_00177632 Crocosphaera watsonii 41.3 51.8 NP_440816 Synechocystis sp. 41.0 52.1 T51092 Synechocystis sp. 40.0 51.5

TABLE-US-00008 TABLE 8 Comparison of PpMSC-1 (SEQ ID NO: 12) to known Mitochondiral substrate carrier proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) NP_194188 A. thaliana 56.6 70.0 T05577 A. thaliana 56.7 69.8 Q66PX4 Saccharum 55.4 69.9 officinarum NP_179836 A. thaliana 54.3 71.4 D84613 A. thaliana 54.5 71.2

TABLE-US-00009 TABLE 9 Comparison of PpMBP-1 (SEQ ID NO: 14) to known MADS-box proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) Q8LPA5 P. patens 63.0 63.0 Q6QAF0 P. patens 62.6 62.6 Q9FE71 P. patens 53.4 55.8 Q9FE89 P. patens 49.1 53.7 Q8LLC8 Lycopodium 46.1 59.5 annotinum

TABLE-US-00010 TABLE 10 Comparison of PpAK-1 (SEQ ID NO: 16) to known Adenosine kinase proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) ADK_PHYPA P. patens 100 100 NP_195950 A. thaliana 66.3 77.8 XP_466836 O. sativa 68.2 77.6 Q84P58 O. sativa 62.9 71.5 NP_187593 A. thaliana 64.7 76.6

TABLE-US-00011 TABLE 11 Comparison of PpZF-6 (SEQ ID NO: 18) to known zinc finger proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) NP_180709 A. thaliana 61.1 71.0 XP_472997 O. sativa 63.4 72.1 NP_176722 A. thaliana 61.8 72.4 T02366 A. thaliana 54.7 64.1 NP_172080 A. thaliana 58.9 69.0

TABLE-US-00012 TABLE 12 Comparison of PpCDK-1 (SEQ ID NO: 20) to known Cyclin-dependent kinase regulatory subunits Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) Q6JJ57 Ipomoea trifida 76.9 83.5 Q6T300 G. max 79.1 82.4 NP_180364 A. thaliana 74.7 82.4 XP_470214 O. sativa 80.2 84.6 Q8GZU5 Populus tremula × 75.8 80.2 P. tremuloides

TABLE-US-00013 TABLE 13 Comparison of PpZF-7 (SEQ ID NO: 22) to known RING zinc finger proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) Q6F3A0 O. sativa 34.7 47.3 Q852N7 O. sativa 35.5 48.5 Q7XJB5 O. sativa 33.1 45.9 NP_851050 A. thaliana 35.3 46.2 NP_851051 A. thaliana 35.3 46.2

TABLE-US-00014 TABLE 14 Comparison of PpMFP-1 (SEQ ID NO: 24) to known MAR binding filament-like protein 1 Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) MFP1_TOBAC Nicotiana tabacum 18.0 30.5 NP_914440 O. sativa 16.8 30.1 MFP1_ARATH A. thaliana 19.2 30.3 NP_188221 A. thaliana 20.1 31.1 T07111 Lycopersicon 19.6 31.6 esculentum

TABLE-US-00015 TABLE 15 Comparison of PpLRP-2 (SEQ ID NO: 26) to known Leucine rich repeat receptor-like protein kinases Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) Q9XGG1 Sorghum bicolor 9.3 16.1 NP_189183 A. thaliana 9.2 14.6 Q708X5 Cicer arietinum 18.0 28.6 XP_474976 O. sativa 5.9 10.3 Q9LSU7 A. thaliana 9.2 14.0

TABLE-US-00016 TABLE 16 Comparison of PpPPK-1 (SEQ ID NO: 28) to known light-sensor protein kinases Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) S27396 Ceratodon purpureus 5.7 11.4 P93098 Ceratodon purpureus 5.8 11.5 PHY1_CERPU Ceratodon purpureus 5.7 11.4 NP_564829 A. thaliana 19.0 32.1 H96666 A. thaliana 19.5 31.6

TABLE-US-00017 TABLE 17 Comparison of PpSRP-1 (SEQ ID NO: 30) to Synaptobrevin-related proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) NP_180871 A. thaliana 73.0 84.2 Q7X9C5 Pyrus pyrifolia 64.9 75.2 NP_180826 A. thaliana 55.4 63.8 Q681H0 A. thaliana 71.2 83.8

TABLE-US-00018 TABLE 18 Comparison of PpCBL-1 (SEQ ID NO: 32) to known Calcineurin B proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) XP_3133573 Anopheles gambiae 19.1 36.7 NP_505885 Caenorhabditis 16.6 34.8 elegans Q95P81 Bombyx mori 18.7 36.4 NP_524874 D. melanogaster 17.1 35.3

TABLE-US-00019 TABLE 19 Comparison of PpCBL-2 (SEQ ID NO: 34) to known caleosin-related proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) Q9SQ57 Sesamum indicum 64.9 78.4 T07092 G. max 67.5 74.6 NP_194404 A. thaliana 61.2 74.7 NP_200335 A. thaliana 62.1 72.8 XP_473140 O. sativa 60.2 73.0

TABLE-US-00020 TABLE 20 Comparison of PpHD-1 (SEQ ID NO: 36) to known histone deacetylase proteins Public Database Sequence Sequence Accession # Species Identity (%) Similarity (%) Q8W508 Zea mays 81.9 88.6 NP_190054 A. thaliana 78.7 88.2 T47443 A. thaliana 76.1 85.8 Q6JJ24 Ipomoea trifida 68.2 75.6 Q7XLX3 Oryza sativa 70.1 77.5

Example 2

Cloning of Full-Length cDNAs from Other Plants

[0109] Canola, soybean, rice, maize, linseed, and wheat plants were grown under a variety of conditions and treatments, and different tissues were harvested at various developmental stages. Plant growth and harvesting were done in a strategic manner such that the probability of harvesting all expressable genes in at least one or more of the resulting libraries is maximized. The mRNA was isolated from each of the collected samples, and cDNA libraries were constructed. No amplification steps were used in the library production process in order to minimize redundancy of genes within the sample and to retain expression information. All libraries were 3' generated from mRNA purified on oligo dT columns. Colonies from the transformation of the cDNA library into E. coli were randomly picked and placed into microtiter plates.

[0110] Plasmid DNA was isolated from the E. coli colonies and then spotted on membranes. A battery of 288 ³³P radiolabeled 7-mer oligonucleotides were sequentially hybridized to these membranes. To increase throughput, duplicate membranes were processed. After each hybridization, a blot image was captured during a phosphorimage scan to generate a hybridization profile for each oligonucleotide. This raw data image was automatically transferred to a computer. Absolute identity was maintained by barcoding for the image cassette, filler, and orientation within the cassette. The filters were then treated using relatively mild conditions to strip the bound probes and returned to the hybridization chambers for another round of hybridization. The hybridization and imaging cycle was repeated until the set of 288 oligomers was completed.

[0111] After completion of the hybridizations, a profile was generated for each spot (representing a cDNA insert), as to which of the 288 ³³P radiolabeled 7-mer oligonucleotides bound to that particular spot (cDNA insert), and to what degree. This profile is defined as the signature generated from that clone. Each clone's signature was compared with all other signatures generated from the same organism to identify clusters of related signatures. This process "sorts" all of the clones from an organism into clusters before sequencing.

[0112] The clones were sorted into various clusters based on their having identical or similar hybridization signatures. A cluster should be indicative of the expression of an individual gene or gene family. A by-product of this analysis is an expression profile for the abundance of each gene in a particular library. One-path sequencing from the 5' end was used to predict the function of the particular clones by similarity and motif searches in sequence databases.

[0113] The full-length DNA sequence of the P. patens PpHD-1 (SEQ ID NO:35) was blasted against proprietary databases of canola, soybean, rice, maize, linseed, and wheat cDNAS at an e value of e^-10 (Altschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). All the contig hits were analyzed for the putative full length sequences, and the longest clones representing the putative full length contigs were fully sequenced. Two homologs from canola (BnHD-1, SEQ ID NO:38 and BnHD-2, SEQ ID NO:40), one homolog from maize (ZmHD-1, SEQ ID NO:42), one homolog from linseed (LuHD-1, SEQ ID NO:44), one sequence from rice (OsHD-1, SEQ ID NO:46) three sequences from soybean (GmHD-1, SEQ ID NO:48, GmHD-2, SEQ ID NO:50, and GmHD-3, SEQ ID NO:52) and one sequence from wheat (TaHD-1, SEQ ID NO:54) were identified. The degree of amino acid identity and similarity of these sequences to the closest known public sequence is indicated in Table 21 (Pairwise Comparison was used: gap penalty; 10; gap extension penalty: 0.1; score matrix: blosum62).

TABLE-US-00021 TABLE 21 Degree of Amino Acid Identity and Similarity of Histone Deacetylases Public Sequence Sequence Gene Name Database Identity Similarity (SEQ ID NO) Accession # Species (%) (%) BnHD-1 NP_190054 A. thaliana 96% 98.1% (SEQ ID NO: 38) BnHD-2 NP_201116 A. thaliana 92.6% 95.3% (SEQ ID NO: 40) ZmHD-1 NP_563817 A. thaliana 66.4% 77.2% (SEQ ID NO: 42) LuHD-1 NP_190054 A. thaliana 87.4% 94.4% (SEQ ID NO: 44) OsHD-1 Q7Y0Y8 O. sativa 100% 100% (SEQ ID NO: 46) GmHD-1 NP_563817 A. thaliana 63.1% 74% (SEQ ID NO: 48) GmHD-2 NP_190054 A. thaliana 85.6% 92.1% (SEQ ID NO: 50) GmHD-2 NP_567921 A. thaliana 67.2% 77.4% (SEQ ID NO: 52) TaHD-1 Q7Y0Y8 O. sativa 89.4% 93.8% (SEQ ID NO: 54)

Example 3

Stress-Tolerant Arabidopsis Plants

[0114] A fragment containing the P. patens polynucleotide was ligated into a binary vector containing a selectable marker gene. The resulting recombinant vector contained the corresponding polynucleotide listed in Table 1 in the sense orientation under the constitutive super promoter. The recombinant vectors were transformed into Agrobacterium tumefaciens C58C1 and PMP90 plants according to standard conditions. A. thaliana ecotype C24 plants were grown and transformed according to standard conditions. T1 plants were screened for resistance to the selection agent conferred by the selectable marker gene, and T1 seeds were collected.

[0115] The P. patens polynucleotides were overexpressed in A. thaliana under the control of a constitutive promoter. T2 and/or T3 seeds were screened for resistance to the selection agent conferred by the selectable marker gene on plates, and positive plants were transplanted into soil and grown in a growth chamber for 3 weeks. Soil moisture was maintained throughout this time at approximately 50% of the maximum water-holding capacity of soil.

[0116] The total water lost (transpiration) by the plant during this time was measured. After 3 weeks, the entire above-ground plant material was collected, dried at 65° C. for 2 days and weighed. The ratio of above-ground plant dry weight (DW) to plant water use is water use efficiency (WUE). Tables 22-41 present WUE and DW for independent transformation events (lines) of transgenic plants overexpressing the P. patens polynucleotides. Least square means (LSM), standard errors, and significant value (P) of a line compared to wild-type controls from an Analysis of Variance are presented. The percent improvement of each P. patens polynucleotides line as compared to wild-type control plants for WUE and DW is also presented.

TABLE-US-00022 TABLE 22 A. thaliana lines overexpressing PpTPT-1 (SEQ ID NO: 2) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.136 0.011 -- -- PpTPT-1 8 0.217 0.025 60 0.0033 (SEQ ID 6 0.222 0.028 64 0.0047 NO: 2) 5 0.226 0.032 67 0.0085 10 0.228 0.025 68 0.0009 2 0.230 0.032 70 0.0063 WUE Wild-type 2.270 0.085 -- -- PpTPT-1 8 2.274 0.190 0 0.9822 (SEQ ID 6 2.308 0.212 2 0.8656 NO: 2) 2 2.426 0.245 7 0.5465 5 2.675 0.245 18 0.1207

TABLE-US-00023 TABLE 23 A. thaliana lines overexpressing PpCDC2-1 (SEQ ID NO: 4) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.088 0.009 -- -- PpCDC2-1 4 0.117 0.016 33 0.1147 (SEQ ID NO: 4) 1 0.125 0.016 42 0.0473 WUE Wild-type 1.446 0.097 -- -- PpCDC2-1 4 1.890 0.186 31 0.036 (SEQ ID NO: 4) 1 1.947 0.186 35 0.0183

TABLE-US-00024 TABLE 24 A. thaliana lines overexpressing PpLRP-1 (SEQ ID NO: 6) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.109 0.033 -- -- PpLRP-1 10 0.161 0.034 48 0.2835 (SEQ ID NO: 6) WUE Wild-type 1.782 0.119 -- -- PpLRP-1 10 2.205 0.169 24 0.0529 (SEQ ID NO: 6)

TABLE-US-00025 TABLE 25 A. thaliana lines overexpressing PpRBP-1 (SEQ ID NO: 8) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.088 0.008 -- -- PpRBP-1 2 0.130 0.017 48 0.0276 (SEQ ID NO: 8) WUE Wild-type 1.446 0.102 -- -- PpRBP-1 2 2.301 0.214 59 0.0004 (SEQ ID NO: 8)

TABLE-US-00026 TABLE 26 A. thaliana lines overexpressing PpPD-1 (SEQ ID NO: 10) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.114 0.006 -- -- PpPD-1 3 0.171 0.019 50 0.0045 (SEQ ID 1 0.171 0.017 50 0.0019 NO: 10) 8 0.174 0.019 53 0.0026 2 0.182 0.019 60 0.0007 11 0.191 0.017 67 <.0001 5 0.201 0.019 76 <.0001 9 0.204 0.017 79 <.0001 WUE Wild-type 1.958 0.058 -- -- PpPD-1 11 2.328 0.165 19 0.0353 (SEQ ID 1 2.343 0.165 20 0.0286 NO: 10) 8 2.354 0.180 20 0.0383 2 2.450 0.180 25 0.0102 3 2.517 0.180 29 0.0036 5 2.552 0.180 30 0.002 9 2.572 0.165 31 0.0006

TABLE-US-00027 TABLE 27 A. thaliana lines overexpressing PpMSC-1 (SEQ ID NO: 12) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.136 0.011 -- -- PpMSC-1 3 0.227 0.027 68 0.0026 (SEQ ID 2 0.240 0.025 77 0.0002 NO: 12) 1 0.247 0.025 82 <.0001 4 0.271 0.022 100 <.0001 WUE Wild-type 2.270 0.085 -- -- PpMSC-1 2 2.343 0.191 3 0.7268 (SEQ ID 4 2.631 0.174 16 0.0654 NO: 12) 1 2.820 0.191 24 0.0097

TABLE-US-00028 TABLE 28 A. thaliana lines overexpressing PpMBP-1 (SEQ ID NO: 14) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.110 0.005 -- -- PpMBP-1 1 0.154 0.017 39 0.0146 (SEQ ID NO: 14) WUE Wild-type 1.620 0.066 -- -- PpMBP-1 1 2.144 0.209 32 0.0182 (SEQ ID NO: 14)

TABLE-US-00029 TABLE 29 A. thaliana lines overexpressing PpAK-1 (SEQ ID NO: 16) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.136 0.011 -- -- PpAK-1 1 0.185 0.022 36 0.049 (SEQ ID 5 0.216 0.022 59 0.0014 NO: 16) 3 0.217 0.027 60 0.0063 7 0.217 0.024 60 0.0026 8 0.227 0.024 68 0.0008 4 0.230 0.024 69 0.0006 WUE Wild-type 2.270 0.084 -- -- PpAK-1 8 2.285 0.188 1 0.9394 (SEQ ID 7 2.358 0.188 4 0.6683 NO: 16) 5 2.374 0.172 5 0.5851 3 2.377 0.211 5 0.6369 4 2.403 0.188 6 0.5191

TABLE-US-00030 TABLE 30 A. thaliana lines overexpressing PpZF-6 (SEQ ID NO: 18) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.107 0.015 -- -- PpZF-6 4 0.131 0.018 22 0.3091 (SEQ ID NO: 18) WUE Wild-type 1.897 0.316 -- -- PpZF-6 4 2.026 0.371 7 0.7946 (SEQ ID NO: 18)

TABLE-US-00031 TABLE 31 A. thaliana lines overexpressing PpCDK-1 (SEQ ID NO: 20) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.088 0.009 -- -- PpCDK-1 7 0.148 0.017 69 0.0029 (SEQ ID NO: 20) WUE Wild-type 1.446 0.102 -- -- PpCDK-1 7 1.963 0.195 36 0.0207 (SEQ ID NO: 20)

TABLE-US-00032 TABLE 32 A. thaliana lines overexpressing PpZF-7 (SEQ ID NO: 22) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.114 0.006 -- -- PpZF-7 1 0.151 0.019 32 0.0617 (SEQ ID 10 0.153 0.019 35 0.0456 NO: 22) 2 0.159 0.019 39 0.0226 7 0.160 0.019 40 0.0198 3 0.163 0.019 43 0.0139 6 0.175 0.019 54 0.0021 9 0.176 0.019 55 0.0018 5 0.177 0.019 56 0.0014 8 0.196 0.019 72 <.0001 4 0.217 0.019 90 <.0001 WUE Wild-type 1.958 0.057 -- -- PpZF-7 2 2.185 0.176 12 0.2224 (SEQ ID 10 2.237 0.176 14 0.1331 NO: 22) 7 2.242 0.176 15 0.1262 9 2.327 0.176 19 0.0479 3 2.359 0.176 20 0.0318 6 2.378 0.176 21 0.0245 1 2.435 0.176 24 0.0108 5 2.490 0.176 27 0.0045 8 2.537 0.176 30 0.002 4 2.707 0.176 38 <.0001

TABLE-US-00033 TABLE 33 A. thaliana lines overexpressing PpMFP-1 (SEQ ID NO: 24) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.110 0.005 -- -- PpMFP-1 4 0.128 0.016 16 0.3008 (SEQ ID 2 0.130 0.016 18 0.25 NO: 24) 10 0.145 0.016 31 0.0465 3 0.146 0.016 32 0.0417 6 0.159 0.016 44 0.0048 7 0.164 0.016 48 0.0022 5 0.166 0.016 50 0.0015 1 0.168 0.016 52 0.0011 8 0.172 0.016 56 0.0004 WUE Wild-type 1.620 0.064 -- -- PpMFP-1 3 1.979 0.203 22 0.0929 (SEQ ID 8 2.049 0.203 26 0.0451 NO: 24) 7 2.049 0.203 26 0.0449 4 2.095 0.203 29 0.0267 1 2.113 0.203 30 0.0215 6 2.178 0.203 34 0.0094 5 2.217 0.203 37 0.0055 10 2.324 0.203 43 0.0011 2 2.345 0.203 45 0.0008

TABLE-US-00034 TABLE 34 A. thaliana lines overexpressing PpLRP-2 (SEQ ID NO: 26) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.136 0.011 -- -- PpLRP-2 4 0.199 0.029 47 0.042 (SEQ ID 2 0.206 0.023 52 0.0078 NO: 26) 3 0.224 0.029 65 0.0049 1 0.227 0.023 67 0.0007 5 0.235 0.026 74 0.0006 8 0.266 0.040 96 0.0026 WUE Wild-type 2.270 0.090 -- -- PpLRP-2 4 2.360 0.224 4 0.7073 (SEQ ID 5 2.402 0.200 6 0.5481 NO: 26) 1 2.404 0.183 6 0.5095 8 2.471 0.317 9 0.5426

TABLE-US-00035 TABLE 35 A. thaliana lines overexpressing PpPPK-1 (SEQ ID NO: 28) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.108 0.007 -- -- PpPPK-1 4 0.157 0.020 45 0.023 (SEQ ID 2 0.159 0.018 47 0.0097 NO: 28) 10 0.175 0.020 62 0.0018 9 0.177 0.022 64 0.0037 WUE Wild-type 1.951 0.078 -- -- PpPPK-1 4 2.043 0.219 5 0.6913 (SEQ ID 9 2.158 0.245 11 0.4225 NO: 28) 2 2.177 0.200 12 0.2948 10 2.523 0.219 29 0.0149

TABLE-US-00036 TABLE 36 A. thaliana lines overexpressing PpSRP-1 (SEQ ID NO: 30) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.114 0.006 -- -- PpSRP-1 7 0.152 0.018 33 0.0495 (SEQ ID 6 0.159 0.018 39 0.0196 NO: 30) 1 0.162 0.020 42 0.026 10 0.164 0.017 44 0.0054 9 0.167 0.015 46 0.0015 8 0.174 0.018 53 0.0019 2 0.179 0.018 57 0.0008 WUE Wild-type 1.958 0.057 -- -- PpSRP-1 10 2.109 0.161 8 0.3776 (SEQ ID 8 2.197 0.177 12 0.1991 NO: 30) 9 2.239 0.149 14 0.0802 2 2.302 0.177 18 0.0659 6 2.405 0.177 23 0.017 1 2.450 0.197 25 0.0178

TABLE-US-00037 TABLE 37 A. thaliana lines overexpressing PpCBL-1 (SEQ ID NO: 32) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.114 0.006 -- -- PpCBL-1 5 0.156 0.019 37 0.034 (SEQ ID 8 0.163 0.019 43 0.0153 NO: 32) 4 0.179 0.019 57 0.0012 3 0.180 0.019 58 0.0011 6 0.181 0.017 59 0.0003 1 0.182 0.017 59 0.0003 9 0.214 0.017 87 <.0001 WUE Wild-type 1.958 0.057 -- -- PpCBL-1 3 2.109 0.177 8 0.4181 (SEQ ID 5 2.152 0.177 10 0.2991 NO: 32) 8 2.158 0.177 10 0.2836 1 2.298 0.162 17 0.0489 6 2.306 0.162 18 0.0438 9 2.319 0.162 18 0.0367 4 2.440 0.177 25 0.0105

TABLE-US-00038 TABLE 38 A. thaliana lines overexpressing PpCBL-2 (SEQ ID NO: 34) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.114 0.006 -- -- PpCBL-2 9 0.156 0.017 37 0.0226 (SEQ ID 10 0.170 0.017 49 0.0027 NO: 34) 1 0.179 0.017 57 0.0005 4 0.188 0.017 65 <.0001 7 0.188 0.017 65 <.0001 3 0.192 0.017 68 <.0001 8 0.194 0.017 70 <.0001 2 0.203 0.017 78 <.0001 WUE Wild-type 1.958 0.054 -- -- PpCBL-2 9 1.944 0.168 -1 0.9357 (SEQ ID 2 2.314 0.168 18 0.0451 NO: 34) 10 2.322 0.168 19 0.0405 8 2.448 0.168 25 0.0061 3 2.545 0.168 30 0.0011 4 2.569 0.168 31 0.0007 1 2.617 0.168 34 0.0003 7 2.771 0.168 42 <.0001

TABLE-US-00039 TABLE 39 A. thaliana lines overexpressing PpHD-1 (SEQ ID NO: 36) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 1.620 0.065 -- -- PpHD-1 7 1.976 0.207 22 0.1027 (SEQ ID 3 1.985 0.207 23 0.0944 NO: 36) 6 2.144 0.207 32 0.0169 2 2.374 0.207 47 0.0007 8 2.444 0.207 51 0.0002 WUE Wild-type 0.110 0.005 -- -- PpHD-1 2 0.126 0.016 14 0.3655 (SEQ ID 8 0.143 0.016 30 0.0566 NO: 36) 6 0.149 0.016 35 0.0246 3 0.152 0.016 37 0.0177 7 0.191 0.016 73 <.0001

TABLE-US-00040 TABLE 40 A. thaliana lines overexpressing PpLRP-1 (SEQ ID NO: 56) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.109 0.033 -- -- PpLRP-1 10 0.161 0.034 48 0.2835 (SEQ ID NO: 56) WUE Wild-type 1.782 0.119 -- -- PpLRP-1 10 2.205 0.169 24 0.0529 (SEQ ID NO: 56)

TABLE-US-00041 TABLE 41 A. thaliana lines overexpressing PpLRP-1 (SEQ ID NO: 58) % Measure- Standard Improve- ment Genotype Line LSM Error ment P DW Wild-type 0.109 0.033 -- -- PpLRP-1 10 0.161 0.034 48 0.2835 (SEQ ID NO: 58) WUE Wild-type 1.782 0.119 -- -- PpLRP-1 10 2.205 0.169 24 0.0529 (SEQ ID NO: 58)

Example 4

Stress-Tolerant Rapeseed/Canola Plants

[0117] Canola cotyledonary petioles of 4 day-old young seedlings are used as explants for tissue culture and transformed according to EP1566443. The commercial cultivar Westar (Agriculture Canada) is the standard variety used for transformation, but other varieties can be used. A. tumefaciens GV3101:pMP90RK containing a binary vector is used for canola transformation. The standard binary vector used for transformation is pSUN (WO 02/00900), but many different binary vector systems have been described for plant transformation (e.g. An, G. In Agrobacterium Protocols, Methods in Molecular Biology vol 44, pp 47-62, Gartland K M A and M R Davey eds. Humana Press, Totowa, N.J.). A plant gene expression cassette comprising a selection marker gene and a plant promoter regulating the transcription of the cDNA encoding the polynucleotide is employed. Various selection marker genes can be used including the mutated acetohydroxy acid synthase (AHAS) gene disclosed in U.S. Pat. Nos. 5,767,366 and 6,225,105. A suitable promoter is used to regulate the trait gene to provide constitutive, developmental, tissue or environmental regulation of gene transcription.

[0118] Canola seeds are surface-sterilized in 70% ethanol for 2 min, incubated for 15 min in 55° C. warm tap water and then in 1.5% sodium hypochlorite for 10 minutes, followed by three rinses with sterilized distilled water. Seeds are then placed on MS medium without hormones, containing Gamborg B5 vitamins, 3% sucrose, and 0.8% Oxoldagar. Seeds are germinated at 24° C. for 4 days in low light (<50 μMol/m²s, 16 hours light). The cotyledon petiole explants with the cotyledon attached are excised from the in vitro seedlings, and inoculated with Agrobacterium by dipping the cut end of the petiole explant into the bacterial suspension. The explants are then cultured for 3 days on MS medium including vitamins containing 3.75 mg/l BAP, 3% sucrose, 0.5 g/l MES, pH 5.2, 0.5 mg/l GA3, 0.8% Oxoldagar at 24° C., 16 hours of light. After three days of co-cultivation with Agrobacterium, the petiole explants are transferred to regeneration medium containing 3.75 mg/l BAP, 0.5 mg/l GA3, 0.5 g/l MES, pH 5.2, 300 mg/l timentin and selection agent until shoot regeneration. As soon as explants start to develop shoots, they are transferred to shoot elongation medium (A6, containing full strength MS medium including vitamins, 2% sucrose, 0.5% Oxoldagar, 100 mg/l myo-inositol, 40 mg/l adenine sulfate, 0.5 g/l MES, pH 5.8, 0.0025 mg/l BAP, 0.1 mg/l IBA, 300 mg/l timentin and selection agent).

[0119] Samples from both in vitro and greenhouse material of the primary transgenic plants (T0) are analyzed by qPCR using TaqMan probes to confirm the presence of T-DNA and to determine the number of T-DNA integrations.

[0120] Seed is produced from the primary transgenic plants by self-pollination. The second-generation plants are grown in greenhouse conditions and self-pollinated. The plants are analyzed by qPCR using TaqMan probes to confirm the presence of T-DNA and to determine the number of T-DNA integrations. Homozygous transgenic, heterozygous transgenic and azygous (null transgenic) plants are compared for their stress tolerance, for example, in the assays described in Example 3, and for yield, both in the greenhouse and in field studies.

Example 5

Screening for Stress-Tolerant Rice Plants

[0121] Transgenic rice plants comprising a polynucleotide of the invention are generated using known methods. Approximately 15 to 20 independent transformants (T0) are generated. The primary transformants are transferred from tissue culture chambers to a greenhouse for growing and harvest of T1 seeds. Five events of the T1 progeny segregated 3:1 for presence/absence of the transgene are retained. For each of these events, 10 T1 seedlings containing the transgene (hetero- and homozygotes), and 10 T1 seedlings lacking the transgene (nullizygotes) are selected by visual marker screening. The selected T1 plants are transferred to a greenhouse. Each plant receives a unique barcode label to link unambiguously the phenotyping data to the corresponding plant. The selected T1 plants are grown on soil in 10 cm diameter pots under the following environmental settings: photoperiod=11.5 h, daylight intensity=30,000 lux or more, daytime temperature=28° C. or higher, night time temperature=22° C., relative humidity=60-70%. Transgenic plants and the corresponding nullizygotes are grown side-by-side at random positions. From the stage of sowing until the stage of maturity, the plants are passed several times through a digital imaging cabinet. At each time point digital, images (2048×1536 pixels, 16 million colours) of each plant are taken from at least 6 different angles.

[0122] The data obtained in the first experiment with T1 plants are confirmed in a second experiment with T2 plants. Lines that have the correct expression pattern are selected for further analysis. Seed batches from the positive plants (both hetero- and homozygotes) in T1 are screened by monitoring marker expression. For each chosen event, the heterozygote seed batches are then retained for T2 evaluation. Within each seed batch, an equal number of positive and negative plants are grown in the greenhouse for evaluation.

[0123] Transgenic plants are screened for their improved growth and/or yield and/or stress tolerance, for example, using the assays described in Example 3, and for yield, both in the greenhouse and in field studies.

Example 6

Stress-Tolerant Soybean Plants

[0124] The polynucleotides of Tables 1 and 2 are transformed into soybean using the methods described in commonly owned copending international application number WO 2005/121345, the contents of which are incorporated herein by reference. The transgenic plants are then screened for their improved growth under water-limited conditions and/or drought, salt, and/or cold tolerance, for example, using the assays described in Example 3, and for yield, both in the greenhouse and in field studies.

Example 7

Stress-Tolerant Wheat Plants

[0125] Transformation of wheat is performed with the method described by Ishida et al., 1996, Nature Biotech. 14745-50. Immature embryos are co-cultivated with Agrobacterium tumefaciens that carry "super binary" vectors, and transgenic plants are recovered through organogenesis. This procedure provides a transformation efficiency between 2.5% and 20%. The transgenic plants are then screened for their improved growth and/or yield under water-limited conditions and/or stress tolerance, for example, is the assays described in Example 3, and for yield, both in the greenhouse and in field studies.

Example 8

Stress-Tolerant Corn Plants

[0126] Agrobacterium cells harboring the genes and the maize ahas gene on the same plasmid are grown in YP medium supplemented with appropriate antibiotics for 1-3 days. A loop of Agrobacterium cells is collected and suspended in 1.5 ml M-LS-002 medium (LS-inf) and the tube containing Agrobacterium cells is kept on a shaker for 1-4 hours at 1,000 rpm.

[0127] Corncobs [genotype J553x(HIIIAxA188)] are harvested at 7-12 days after pollination. The cobs are sterilized in 20% Clorox solution for 15 minutes followed by thorough rinse with sterile water. Immature embryos with size 0.8-2.0 mm are dissected into the tube containing Agrobacterium cells in LS-inf solution.

[0128] Agro-infection is carried out by keeping the tube horizontally in the laminar hood at room temperature for 30 minutes. Mixture of the agro infection is poured on to a plate containing the co-cultivation medium (M-LS-011). After the liquid agro-solution is piped out, the embryos transferred to the surface of a filter paper that is placed on the agar co-cultivation medium. The excess bacterial solution is removed with a pipette. The embryos are placed on the co-cultivation medium with scutellum side up and cultured in the dark at 22° C. for 2-4 days.

[0129] Embryos are transferred to M-MS-101 medium without selection. Seven to ten days later, embryos are transferred to M-LS-401 medium containing 0.50 μM imazethapyr and grown for 4 weeks (two 2-week transfers) to select for transformed callus cells. Plant regeneration is initiated by transferring resistant calli to M-LS-504 medium supplemented with 0.75 μM imazethapyr and grown under light at 25-27° C. for two to three weeks. Regenerated shoots are then transferred to rooting box with M-MS-618 medium (0.5 μM imazethapyr). Plantlets with roots are transferred to potting mixture in small pots in the greenhouse and after acclimatization are then transplanted to larger pots and maintained in greenhouse till maturity.

[0130] The copy number of the transgene in each plantlet is assayed using Taqman analysis of genomic DNA, and transgene expression is assayed using qRT-PCR of total RNA isolated from leaf samples.

[0131] Using assays such as the assay described in Example 3, each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. Transgene positive and negative plants are marked and paired with similar sizes for transplanting together to large pots. This provides a uniform and competitive environment for the transgene positive and negative plants. The large pots are watered to a certain percentage of the field water capacity of the soil depending the severity of water-stress desired. The soil water level is maintained by watering every other day. Plant growth and physiology traits such as height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the growth period. After a period of growth, the above ground portion of the plants is harvested, and the fresh weight and dry weight of each plant are taken. A comparison of the drought tolerance phenotype between the transgene positive and negative plants is then made.

[0132] Using assays such as the assay described in Example 3, the pots are covered with caps that permit the seedlings to grow through but minimize water loss. Each pot is weighed periodically and water added to maintain the initial water content. At the end of the experiment, the fresh and dry weight of each plant is measured, the water consumed by each plant is calculated and WUE of each plant is computed. Plant growth and physiology traits such as WUE, height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the experiment. A comparison of WUE phenotype between the transgene positive and negative plants is then made.

[0133] Using assays such as the assay described in Example 3, these pots are kept in an area in the greenhouse that has uniform environmental conditions, and cultivated optimally. Each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. The plants are allowed to grow under theses conditions until they reach a predefined growth stage. Water is then withheld. Plant growth and physiology traits such as height, stem diameter, leaf rolling, plant wilting, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured as stress intensity increases. A comparison of the dessication tolerance phenotype between transgene positive and negative plants is then made.

[0134] Segregating transgenic corn seeds for a transformation event are planted in small pots for testing in a cycling drought assay. These pots are kept in an area in the greenhouse that has uniform environmental conditions, and cultivated optimally. Each of these plants is uniquely labeled, sampled and analyzed for transgene copy number. The plants are allowed to grow under theses conditions until they reach a predefined growth stage. Plants are then repeatedly watered to saturation at a fixed interval of time. This water/drought cycle is repeated for the duration of the experiment. Plant growth and physiology traits such as height, stem diameter, leaf rolling, leaf extension rate, leaf water status, chlorophyll content and photosynthesis rate are measured during the growth period. At the end of the experiment, the plants are harvested for above-ground fresh and dry weight. A comparison of the cycling drought tolerance phenotype between transgene positive and negative plants is then made.

[0135] In order to test segregating transgenic corn for drought tolerance under rain-free conditions, managed-drought stress at a single location or multiple locations is used. Crop water availability is controlled by drip tape or overhead irrigation at a location which has less than 10 cm rainfall and minimum temperatures greater than 5° C. expected during an average 5 month season, or a location with expected in-season precipitation intercepted by an automated "rain-out shelter" which retracts to provide open field conditions when not required. Standard agronomic practices in the area are followed for soil preparation, planting, fertilization and pest control. Each plot is sown with seed segregating for the presence of a single transgenic insertion event. A Taqman transgene copy number assay is used on leaf samples to differentiate the transgenics from null-segregant control plants. Plants that have been genotyped in this manner are also scored for a range of phenotypes related to drought-tolerance, growth and yield. These phenotypes include plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO₂ uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plants are used as the replicate unit per event.

[0136] In order to test non-segregating transgenic corn for drought tolerance under rain-free conditions, managed-drought stress at a single location or multiple locations is used. Crop water availability is controlled by drip tape or overhead irrigation at a location which has less than 10 cm rainfall and minimum temperatures greater than 5° C. expected during an average 5 month season, or a location with expected in-season precipitation intercepted by an automated "rain-out shelter" which retracts to provide open field conditions when not required. Standard agronomic practices in the area are followed for soil preparation, planting, fertilization and pest control. Trial layout is designed to pair a plot containing a non-segregating transgenic event with an adjacent plot of null-segregant controls. A null segregant is progeny (or lines derived from the progeny) of a transgenic plant that does not contain the transgene due to Mendelian segregation. Additional replicated paired plots for a particular event are distributed around the trial. A range of phenotypes related to drought-tolerance, growth and yield are scored in the paired plots and estimated at the plot level. When the measurement technique could only be applied to individual plants, these are selected at random each time from within the plot. These phenotypes include plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO₂ uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plots are used as the replicate unit per event.

[0137] To perform multi-location testing of transgenic corn for drought tolerance and yield, five to twenty locations encompassing major corn growing regions are selected. These are widely distributed to provide a range of expected crop water availabilities based on average temperature, humidity, precipitation and soll type. Crop water availability is not modified beyond standard agronomic practices. Trial layout is designed to pair a plot containing a non-segregating transgenic event with an adjacent plot of null-segregant controls. A range of phenotypes related to drought-tolerance, growth and yield are scored in the paired plots and estimated at the plot level. When the measurement technique could only be applied to individual plants, these are selected at random each time from within the plot. These phenotypes included plant height, grain weight per plant, grain number per plant, ear number per plant, above ground dry-weight, leaf conductance to water vapor, leaf CO₂ uptake, leaf chlorophyll content, photosynthesis-related chlorophyll fluorescence parameters, water use efficiency, leaf water potential, leaf relative water content, stem sap flow rate, stem hydraulic conductivity, leaf temperature, leaf reflectance, leaf light absorptance, leaf area, days to flowering, anthesis-silking interval, duration of grain fill, osmotic potential, osmotic adjustment, root size, leaf extension rate, leaf angle, leaf rolling and survival. All measurements are made with commercially available instrumentation for field physiology, using the standard protocols provided by the manufacturers. Individual plots are used as the replicate unit per event.

Sequence CWU 1

1

5411350DNAPhyscomitrella patensCDS(133)..(1101) 1atcccgggtg tctctcagtt gccatcgtgg agtcagcgag cacgcttttg tgtatctctg 60cgcggagttt tcttttggaa gcggctaaag tatatcagcg tggtcgatag ggctgactcg 120ttttctgtag tc atg gct ggc aga gga gga aag aga ttc tcc ttt gga gga 171 Met Ala Gly Arg Gly Gly Lys Arg Phe Ser Phe Gly Gly 1 5 10 atg ggt agt gca gct gct ttc gga ggt gga gag ggt agc agg gtt gtg 219Met Gly Ser Ala Ala Ala Phe Gly Gly Gly Glu Gly Ser Arg Val Val 15 20 25 gga gat ggt tct act gct cca gag tca tcg tcc aga cag cat gaa tac 267Gly Asp Gly Ser Thr Ala Pro Glu Ser Ser Ser Arg Gln His Glu Tyr 30 35 40 45 cga caa cca gcg gct act aga gag cgt ttt ggg gag gta gaa cac gaa 315Arg Gln Pro Ala Ala Thr Arg Glu Arg Phe Gly Glu Val Glu His Glu 50 55 60 gat gaa gat gta tca ggt tca ggt gct ggt ggt agt caa gtg agg cca 363Asp Glu Asp Val Ser Gly Ser Gly Ala Gly Gly Ser Gln Val Arg Pro 65 70 75 tca ggg ggt gga ggt cga ggt cga ggc cga ggc cga ggt cga ggg aga 411Ser Gly Gly Gly Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg 80 85 90 agg gat gct atg gat tca acg gaa gct ctg ggg aga tgc atg act gcg 459Arg Asp Ala Met Asp Ser Thr Glu Ala Leu Gly Arg Cys Met Thr Ala 95 100 105 att cta gga cat cga gcc tcg gac tat gga cta gaa atg cag aac gat 507Ile Leu Gly His Arg Ala Ser Asp Tyr Gly Leu Glu Met Gln Asn Asp 110 115 120 125 ggt ttc gtc tta gta gct gat ctt tta aaa cta agc aag aat act gca 555Gly Phe Val Leu Val Ala Asp Leu Leu Lys Leu Ser Lys Asn Thr Ala 130 135 140 gct ggt att cca tta agt tct cac agc gtg gaa gat gtg cgc aag gct 603Ala Gly Ile Pro Leu Ser Ser His Ser Val Glu Asp Val Arg Lys Ala 145 150 155 gtt gca agg gat ggg aaa cga cgt ttt gga cta aaa gaa gag gat ggg 651Val Ala Arg Asp Gly Lys Arg Arg Phe Gly Leu Lys Glu Glu Asp Gly 160 165 170 cat ctt tac atc agg gca aat caa ggt cat agt atc agg acc gtg gaa 699His Leu Tyr Ile Arg Ala Asn Gln Gly His Ser Ile Arg Thr Val Glu 175 180 185 tct gga caa ctt ttg tcg ttg gtt aca tct cct tca caa att cca gtc 747Ser Gly Gln Leu Leu Ser Leu Val Thr Ser Pro Ser Gln Ile Pro Val 190 195 200 205 tgt gtt cat ggc acg tac gag aga ttt atg gac agt atc tgg caa gaa 795Cys Val His Gly Thr Tyr Glu Arg Phe Met Asp Ser Ile Trp Gln Glu 210 215 220 ggg tta aaa cgc atg aat cga aat cat gtt cat ttt gct act ggc ttg 843Gly Leu Lys Arg Met Asn Arg Asn His Val His Phe Ala Thr Gly Leu 225 230 235 cct gaa cag gac ggt gtc atc agt ggg atg cgt gga tct gct cag gtt 891Pro Glu Gln Asp Gly Val Ile Ser Gly Met Arg Gly Ser Ala Gln Val 240 245 250 ctc ata tac ctg gat gtg gag aag gct atg gag gat gga atg aag ctc 939Leu Ile Tyr Leu Asp Val Glu Lys Ala Met Glu Asp Gly Met Lys Leu 255 260 265 tac gtt tca gat aac aaa gtc gtt ctc acc gaa ggc ttt gat ggg gtg 987Tyr Val Ser Asp Asn Lys Val Val Leu Thr Glu Gly Phe Asp Gly Val 270 275 280 285 gtt ccg act aaa tat ttt aag aat gtc gtc aag aag ttg cct cgt ggc 1035Val Pro Thr Lys Tyr Phe Lys Asn Val Val Lys Lys Leu Pro Arg Gly 290 295 300 aga gag atg cca ctc cat cca tca agt aat cag cct aag cct cat gag 1083Arg Glu Met Pro Leu His Pro Ser Ser Asn Gln Pro Lys Pro His Glu 305 310 315 aat act gca gca gat gtc tagaaaacag agaacccaga attggtgatc 1131Asn Thr Ala Ala Asp Val 320 aaagaggatg cctatcagaa tgccagcagc tcccttgctt aatgtctgat tatttctagt 1191ggtttttgtg gaatcaattg caccaaatgc cctgaactta ttggaagaat acaccaactt 1251tctggattta gttatgtaaa tgggagcaga tcgttgaaac tcatgttaca ttatcgacct 1311gatttattag gagagacagc tatcaaccat agagctcgc 13502323PRTPhyscomitrella patens 2Met Ala Gly Arg Gly Gly Lys Arg Phe Ser Phe Gly Gly Met Gly Ser 1 5 10 15 Ala Ala Ala Phe Gly Gly Gly Glu Gly Ser Arg Val Val Gly Asp Gly 20 25 30 Ser Thr Ala Pro Glu Ser Ser Ser Arg Gln His Glu Tyr Arg Gln Pro 35 40 45 Ala Ala Thr Arg Glu Arg Phe Gly Glu Val Glu His Glu Asp Glu Asp 50 55 60 Val Ser Gly Ser Gly Ala Gly Gly Ser Gln Val Arg Pro Ser Gly Gly 65 70 75 80 Gly Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg Arg Asp Ala 85 90 95 Met Asp Ser Thr Glu Ala Leu Gly Arg Cys Met Thr Ala Ile Leu Gly 100 105 110 His Arg Ala Ser Asp Tyr Gly Leu Glu Met Gln Asn Asp Gly Phe Val 115 120 125 Leu Val Ala Asp Leu Leu Lys Leu Ser Lys Asn Thr Ala Ala Gly Ile 130 135 140 Pro Leu Ser Ser His Ser Val Glu Asp Val Arg Lys Ala Val Ala Arg 145 150 155 160 Asp Gly Lys Arg Arg Phe Gly Leu Lys Glu Glu Asp Gly His Leu Tyr 165 170 175 Ile Arg Ala Asn Gln Gly His Ser Ile Arg Thr Val Glu Ser Gly Gln 180 185 190 Leu Leu Ser Leu Val Thr Ser Pro Ser Gln Ile Pro Val Cys Val His 195 200 205 Gly Thr Tyr Glu Arg Phe Met Asp Ser Ile Trp Gln Glu Gly Leu Lys 210 215 220 Arg Met Asn Arg Asn His Val His Phe Ala Thr Gly Leu Pro Glu Gln 225 230 235 240 Asp Gly Val Ile Ser Gly Met Arg Gly Ser Ala Gln Val Leu Ile Tyr 245 250 255 Leu Asp Val Glu Lys Ala Met Glu Asp Gly Met Lys Leu Tyr Val Ser 260 265 270 Asp Asn Lys Val Val Leu Thr Glu Gly Phe Asp Gly Val Val Pro Thr 275 280 285 Lys Tyr Phe Lys Asn Val Val Lys Lys Leu Pro Arg Gly Arg Glu Met 290 295 300 Pro Leu His Pro Ser Ser Asn Gln Pro Lys Pro His Glu Asn Thr Ala 305 310 315 320 Ala Asp Val 3980DNAPhyscomitrella patensCDS(37)..(918) 3gcgttaacgg tctgatctcg ttacctcaag tttcaa atg gat cag tat gag aaa 54 Met Asp Gln Tyr Glu Lys 1 5 gtg gag aag att gga gag ggc aca tac ggt gtc gta tac aag gcc cgg 102Val Glu Lys Ile Gly Glu Gly Thr Tyr Gly Val Val Tyr Lys Ala Arg 10 15 20 gat cgc ctc act aat gaa act att gct ctg aaa aaa ata cgg ctg gag 150Asp Arg Leu Thr Asn Glu Thr Ile Ala Leu Lys Lys Ile Arg Leu Glu 25 30 35 caa gaa gat gaa ggt gtt cca agc acc gcc att cga gaa att tca ctc 198Gln Glu Asp Glu Gly Val Pro Ser Thr Ala Ile Arg Glu Ile Ser Leu 40 45 50 ctg aaa gaa atg cac cat ggc aac atc gtt cgg cta caa gat gtg gtg 246Leu Lys Glu Met His His Gly Asn Ile Val Arg Leu Gln Asp Val Val 55 60 65 70 cat agt gag aaa cga ttg tac ttg gtt ttt gaa tac ctg gac ctc gac 294His Ser Glu Lys Arg Leu Tyr Leu Val Phe Glu Tyr Leu Asp Leu Asp 75 80 85 cta aag aag cat atg gac acc tgc ccg gac ctt gcc aaa gac cca cgc 342Leu Lys Lys His Met Asp Thr Cys Pro Asp Leu Ala Lys Asp Pro Arg 90 95 100 ttg atc aag acc ttt cta tac cag atc ttg cgg ggc att gct tat tgc 390Leu Ile Lys Thr Phe Leu Tyr Gln Ile Leu Arg Gly Ile Ala Tyr Cys 105 110 115 cat gcc cac agg gtc ctt cac aga gac ttg aaa cct cag aat ctt ttg 438His Ala His Arg Val Leu His Arg Asp Leu Lys Pro Gln Asn Leu Leu 120 125 130 att gat cga cgt acc aat gct ttg aag cta gct gac ttt ggc ctt gca 486Ile Asp Arg Arg Thr Asn Ala Leu Lys Leu Ala Asp Phe Gly Leu Ala 135 140 145 150 cga gct ttt ggt att cct gtc agg aca ttc act cat gag gtg gta aca 534Arg Ala Phe Gly Ile Pro Val Arg Thr Phe Thr His Glu Val Val Thr 155 160 165 ttg tgg tac aga gca cca gaa att ctc tta ggt tct cgc cac tac tct 582Leu Trp Tyr Arg Ala Pro Glu Ile Leu Leu Gly Ser Arg His Tyr Ser 170 175 180 aca cct gta gat gtg tgg tct gta gga tgc att ttc gct gag atg gtc 630Thr Pro Val Asp Val Trp Ser Val Gly Cys Ile Phe Ala Glu Met Val 185 190 195 aat caa cgg ccc ttg ttt cca gga gat tct gag ata gat gag ctg ttc 678Asn Gln Arg Pro Leu Phe Pro Gly Asp Ser Glu Ile Asp Glu Leu Phe 200 205 210 aaa atc ttc agg aca ctt ggc act cca aat gaa gaa gtt tgg cca ggt 726Lys Ile Phe Arg Thr Leu Gly Thr Pro Asn Glu Glu Val Trp Pro Gly 215 220 225 230 gta act tca ttg cca gat ttc aag act gct ttt cca aag tgg cct ccg 774Val Thr Ser Leu Pro Asp Phe Lys Thr Ala Phe Pro Lys Trp Pro Pro 235 240 245 aag cct ttg tca tca gtc gta cct agc ctt gag cca gca ggc atc gac 822Lys Pro Leu Ser Ser Val Val Pro Ser Leu Glu Pro Ala Gly Ile Asp 250 255 260 ttg cta gag aaa atg ctg aca ctt gag cca agt cga cgg gta aca gca 870Leu Leu Glu Lys Met Leu Thr Leu Glu Pro Ser Arg Arg Val Thr Ala 265 270 275 cga aat gca ttg gaa cac gag tat ttc aag gat atc ggt ctt gta ccc 918Arg Asn Ala Leu Glu His Glu Tyr Phe Lys Asp Ile Gly Leu Val Pro 280 285 290 tgattcttgt atgcaacttg tatgatactg tctagggtat ccctgcaagg cagcgagctc 978gc 9804294PRTPhyscomitrella patens 4Met Asp Gln Tyr Glu Lys Val Glu Lys Ile Gly Glu Gly Thr Tyr Gly 1 5 10 15 Val Val Tyr Lys Ala Arg Asp Arg Leu Thr Asn Glu Thr Ile Ala Leu 20 25 30 Lys Lys Ile Arg Leu Glu Gln Glu Asp Glu Gly Val Pro Ser Thr Ala 35 40 45 Ile Arg Glu Ile Ser Leu Leu Lys Glu Met His His Gly Asn Ile Val 50 55 60 Arg Leu Gln Asp Val Val His Ser Glu Lys Arg Leu Tyr Leu Val Phe 65 70 75 80 Glu Tyr Leu Asp Leu Asp Leu Lys Lys His Met Asp Thr Cys Pro Asp 85 90 95 Leu Ala Lys Asp Pro Arg Leu Ile Lys Thr Phe Leu Tyr Gln Ile Leu 100 105 110 Arg Gly Ile Ala Tyr Cys His Ala His Arg Val Leu His Arg Asp Leu 115 120 125 Lys Pro Gln Asn Leu Leu Ile Asp Arg Arg Thr Asn Ala Leu Lys Leu 130 135 140 Ala Asp Phe Gly Leu Ala Arg Ala Phe Gly Ile Pro Val Arg Thr Phe 145 150 155 160 Thr His Glu Val Val Thr Leu Trp Tyr Arg Ala Pro Glu Ile Leu Leu 165 170 175 Gly Ser Arg His Tyr Ser Thr Pro Val Asp Val Trp Ser Val Gly Cys 180 185 190 Ile Phe Ala Glu Met Val Asn Gln Arg Pro Leu Phe Pro Gly Asp Ser 195 200 205 Glu Ile Asp Glu Leu Phe Lys Ile Phe Arg Thr Leu Gly Thr Pro Asn 210 215 220 Glu Glu Val Trp Pro Gly Val Thr Ser Leu Pro Asp Phe Lys Thr Ala 225 230 235 240 Phe Pro Lys Trp Pro Pro Lys Pro Leu Ser Ser Val Val Pro Ser Leu 245 250 255 Glu Pro Ala Gly Ile Asp Leu Leu Glu Lys Met Leu Thr Leu Glu Pro 260 265 270 Ser Arg Arg Val Thr Ala Arg Asn Ala Leu Glu His Glu Tyr Phe Lys 275 280 285 Asp Ile Gly Leu Val Pro 290 52497DNAPhyscomitrella patensCDS(144)..(2081) 5gcgttaacga gctagtatct gttcctccat aacagaatgg gtaaaacaag gtgacgtgtc 60atgcagggac tctccttgcg tcgaagtctc acaagcaagt ccacgctgaa ttatttgaat 120ttgatacaac aggcgtaaat aag atg aaa aac gat gag gcc cat agc cac gat 173 Met Lys Asn Asp Glu Ala His Ser His Asp 1 5 10 aac cac ata tcc ggc ggt atg aag gct gcc gaa gca ttt gtg gaa ggt 221Asn His Ile Ser Gly Gly Met Lys Ala Ala Glu Ala Phe Val Glu Gly 15 20 25 gca tac gag ggt gac gag gag gaa gag ttt gag aaa gac cga aat tct 269Ala Tyr Glu Gly Asp Glu Glu Glu Glu Phe Glu Lys Asp Arg Asn Ser 30 35 40 cgc agt atc cgg act ggg cgt cat tcc gag gga tca cgt agt ggt cag 317Arg Ser Ile Arg Thr Gly Arg His Ser Glu Gly Ser Arg Ser Gly Gln 45 50 55 ctt ttt cca gat gaa cgg cac tca ggg tct agt gcg ggc gat gca tct 365Leu Phe Pro Asp Glu Arg His Ser Gly Ser Ser Ala Gly Asp Ala Ser 60 65 70 gct aca tat tat gag ctc cac tcc aac atg gct tgc aag tct ggc aca 413Ala Thr Tyr Tyr Glu Leu His Ser Asn Met Ala Cys Lys Ser Gly Thr 75 80 85 90 gca gca ggg cat atc ttt gat gag gaa ggt gtt gga gat tac gcc agt 461Ala Ala Gly His Ile Phe Asp Glu Glu Gly Val Gly Asp Tyr Ala Ser 95 100 105 gat cca ggt gtt tac cat gat gac tcc tgt ctg aac cca ttg gaa aaa 509Asp Pro Gly Val Tyr His Asp Asp Ser Cys Leu Asn Pro Leu Glu Lys 110 115 120 gac ttg gag gat gat caa ctc tgc cat ggt gaa gat gca gat cac ttc 557Asp Leu Glu Asp Asp Gln Leu Cys His Gly Glu Asp Ala Asp His Phe 125 130 135 ctc aag aag gcc cgt agt gaa gga ggt ctg tac gaa ctg ggg ctt ata 605Leu Lys Lys Ala Arg Ser Glu Gly Gly Leu Tyr Glu Leu Gly Leu Ile 140 145 150 tct cag caa ctt aca ggt caa tca act gaa caa gat ttg gca cat cat 653Ser Gln Gln Leu Thr Gly Gln Ser Thr Glu Gln Asp Leu Ala His His 155 160 165 170 tct caa gga agc cca tca tat caa ggt atc agc aga cag gac tcc tcc 701Ser Gln Gly Ser Pro Ser Tyr Gln Gly Ile Ser Arg Gln Asp Ser Ser 175 180 185 att cac ttg cca aaa ggt cta gtt gaa ggt ccc cac tca gaa att gat 749Ile His Leu Pro Lys Gly Leu Val Glu Gly Pro His Ser Glu Ile Asp 190 195 200 caa aga gat gcc aaa gat ctt ttc ttg aat gag agg tct tct gac aaa 797Gln Arg Asp Ala Lys Asp Leu Phe Leu Asn Glu Arg Ser Ser Asp Lys 205 210 215 gat gtc gat tac tgc aat ggt tcc tca agg ttg gaa ttt gac gca tat 845Asp Val Asp Tyr Cys Asn Gly Ser Ser Arg Leu Glu Phe Asp Ala Tyr 220 225 230 tat ccc agg agt gat gtt cat aac ccc gag agc ata cgt agt gga tct 893Tyr Pro Arg Ser Asp Val His Asn Pro Glu Ser Ile Arg Ser Gly Ser 235 240 245 250 ttc tta caa aaa gat gac att gcg gaa ttt gat gct gac aat gtt aag 941Phe Leu Gln Lys Asp Asp Ile Ala Glu Phe Asp Ala Asp Asn Val Lys 255 260

265 tca cat aat tcg gct gga gtt gat gga gtc cct gat ggt tgc ata tct 989Ser His Asn Ser Ala Gly Val Asp Gly Val Pro Asp Gly Cys Ile Ser 270 275 280 ggt cac ttt caa gat ttg aac ttg gat tta gtt gct ggg cac gat gag 1037Gly His Phe Gln Asp Leu Asn Leu Asp Leu Val Ala Gly His Asp Glu 285 290 295 aat gac cac act aag caa gac gcg aga gcg tct gag cta gac cgg cct 1085Asn Asp His Thr Lys Gln Asp Ala Arg Ala Ser Glu Leu Asp Arg Pro 300 305 310 aat ttg tca agg gtt gag gag tgg att agg agt ata gaa cca act ccc 1133Asn Leu Ser Arg Val Glu Glu Trp Ile Arg Ser Ile Glu Pro Thr Pro 315 320 325 330 ttc tta gca gat gaa gaa gtt gag ccc aca gct tac tca gat aca gag 1181Phe Leu Ala Asp Glu Glu Val Glu Pro Thr Ala Tyr Ser Asp Thr Glu 335 340 345 cct tca gca ccg gcc gct tcc ttt ttc cgg gct aga gct cga cct gat 1229Pro Ser Ala Pro Ala Ala Ser Phe Phe Arg Ala Arg Ala Arg Pro Asp 350 355 360 cag atg cat cta gat gga atc gct ctt gtg gac cgt aga aac cat cag 1277Gln Met His Leu Asp Gly Ile Ala Leu Val Asp Arg Arg Asn His Gln 365 370 375 gga gag caa ctg ata gat gct gac agt gaa atg gca agc ttt att gct 1325Gly Glu Gln Leu Ile Asp Ala Asp Ser Glu Met Ala Ser Phe Ile Ala 380 385 390 cgg tct gtg aat cca ctt tgt aca gtg gct cat ttc tcg gga gtg gga 1373Arg Ser Val Asn Pro Leu Cys Thr Val Ala His Phe Ser Gly Val Gly 395 400 405 410 tta aag tta cct cca ccc ctt ggc gcg cac aat aat ttg aaa act ctc 1421Leu Lys Leu Pro Pro Pro Leu Gly Ala His Asn Asn Leu Lys Thr Leu 415 420 425 aac ctc tct gcc aac gct atc gta cgc atg tta ccc ggg tgt ctt cca 1469Asn Leu Ser Ala Asn Ala Ile Val Arg Met Leu Pro Gly Cys Leu Pro 430 435 440 aag agc tta cat aca ttg gat ttg tca cga aat aag ata gtt gtg ata 1517Lys Ser Leu His Thr Leu Asp Leu Ser Arg Asn Lys Ile Val Val Ile 445 450 455 gaa ggg ctc cgt gaa ctc tct cga ctc cgt gtg ctg aac cta tca cat 1565Glu Gly Leu Arg Glu Leu Ser Arg Leu Arg Val Leu Asn Leu Ser His 460 465 470 aat cga att att cga att gga cat ggt ttg gcg aac tgt act tct ttg 1613Asn Arg Ile Ile Arg Ile Gly His Gly Leu Ala Asn Cys Thr Ser Leu 475 480 485 490 agg gaa atc tat ttg gct ggt aac aag att agc gag att gag gga cta 1661Arg Glu Ile Tyr Leu Ala Gly Asn Lys Ile Ser Glu Ile Glu Gly Leu 495 500 505 cat cga cta ctg aaa ctt agc ttc att gat ttg agt ttc aac aaa atc 1709His Arg Leu Leu Lys Leu Ser Phe Ile Asp Leu Ser Phe Asn Lys Ile 510 515 520 gcc tca gct aaa tct att ggg cag cta gcc gcc aac tac aat tcc ctc 1757Ala Ser Ala Lys Ser Ile Gly Gln Leu Ala Ala Asn Tyr Asn Ser Leu 525 530 535 cag gca atc aac ctt ttg gga aat cca tta cac agc aac ctt ggt gag 1805Gln Ala Ile Asn Leu Leu Gly Asn Pro Leu His Ser Asn Leu Gly Glu 540 545 550 gag cca tta cgg aaa ttg atc gtt gga ctt act ccg cat gta gtg tac 1853Glu Pro Leu Arg Lys Leu Ile Val Gly Leu Thr Pro His Val Val Tyr 555 560 565 570 ctt aac aaa caa gct acg aag gcc gta tct gca cga gat gct tca gtg 1901Leu Asn Lys Gln Ala Thr Lys Ala Val Ser Ala Arg Asp Ala Ser Val 575 580 585 gat agc gtg gcc aga gct gct ttg gca aat cct agt cac cac act cac 1949Asp Ser Val Ala Arg Ala Ala Leu Ala Asn Pro Ser His His Thr His 590 595 600 caa cga gga aag acc tct tca caa agc aaa tcc ttc ggc gaa gcg gtg 1997Gln Arg Gly Lys Thr Ser Ser Gln Ser Lys Ser Phe Gly Glu Ala Val 605 610 615 cgc ctg ccc cat cca ctg ctt cca gcc ccc gtc acc ggg aca aac gga 2045Arg Leu Pro His Pro Leu Leu Pro Ala Pro Val Thr Gly Thr Asn Gly 620 625 630 ctg gcg aga agt ctg cag cag gtc gta cca atg cac tgaagagtag 2091Leu Ala Arg Ser Leu Gln Gln Val Val Pro Met His 635 640 645 aacgccctct gagctgcctc ctcgacatcg atattctcac agccggcttg ttcatggctc 2151tggggtcact aaggatcacc ctcgtttagc ccatcttcca atgcctccac cagtccagaa 2211ttttatcaga gccgaggaaa aggtttaatt gacctgacgt ttgcaatatt gttgcagtcg 2271gagacaagac tagagggagg aagtttggca agtttgtccg tttggtttgg tgttcacaat 2331atctgcagag ttgtgttagc aacctggtca tttctgtttc agtgtacggt atgctagtgc 2391tctaaaagtt tgatatgttc atgccataat gaataccctg tgggcatttt cattttaata 2451ttgctgcctc aattaattga tgagattctc tggcatgagt taacgc 24976646PRTPhyscomitrella patens 6Met Lys Asn Asp Glu Ala His Ser His Asp Asn His Ile Ser Gly Gly 1 5 10 15 Met Lys Ala Ala Glu Ala Phe Val Glu Gly Ala Tyr Glu Gly Asp Glu 20 25 30 Glu Glu Glu Phe Glu Lys Asp Arg Asn Ser Arg Ser Ile Arg Thr Gly 35 40 45 Arg His Ser Glu Gly Ser Arg Ser Gly Gln Leu Phe Pro Asp Glu Arg 50 55 60 His Ser Gly Ser Ser Ala Gly Asp Ala Ser Ala Thr Tyr Tyr Glu Leu 65 70 75 80 His Ser Asn Met Ala Cys Lys Ser Gly Thr Ala Ala Gly His Ile Phe 85 90 95 Asp Glu Glu Gly Val Gly Asp Tyr Ala Ser Asp Pro Gly Val Tyr His 100 105 110 Asp Asp Ser Cys Leu Asn Pro Leu Glu Lys Asp Leu Glu Asp Asp Gln 115 120 125 Leu Cys His Gly Glu Asp Ala Asp His Phe Leu Lys Lys Ala Arg Ser 130 135 140 Glu Gly Gly Leu Tyr Glu Leu Gly Leu Ile Ser Gln Gln Leu Thr Gly 145 150 155 160 Gln Ser Thr Glu Gln Asp Leu Ala His His Ser Gln Gly Ser Pro Ser 165 170 175 Tyr Gln Gly Ile Ser Arg Gln Asp Ser Ser Ile His Leu Pro Lys Gly 180 185 190 Leu Val Glu Gly Pro His Ser Glu Ile Asp Gln Arg Asp Ala Lys Asp 195 200 205 Leu Phe Leu Asn Glu Arg Ser Ser Asp Lys Asp Val Asp Tyr Cys Asn 210 215 220 Gly Ser Ser Arg Leu Glu Phe Asp Ala Tyr Tyr Pro Arg Ser Asp Val 225 230 235 240 His Asn Pro Glu Ser Ile Arg Ser Gly Ser Phe Leu Gln Lys Asp Asp 245 250 255 Ile Ala Glu Phe Asp Ala Asp Asn Val Lys Ser His Asn Ser Ala Gly 260 265 270 Val Asp Gly Val Pro Asp Gly Cys Ile Ser Gly His Phe Gln Asp Leu 275 280 285 Asn Leu Asp Leu Val Ala Gly His Asp Glu Asn Asp His Thr Lys Gln 290 295 300 Asp Ala Arg Ala Ser Glu Leu Asp Arg Pro Asn Leu Ser Arg Val Glu 305 310 315 320 Glu Trp Ile Arg Ser Ile Glu Pro Thr Pro Phe Leu Ala Asp Glu Glu 325 330 335 Val Glu Pro Thr Ala Tyr Ser Asp Thr Glu Pro Ser Ala Pro Ala Ala 340 345 350 Ser Phe Phe Arg Ala Arg Ala Arg Pro Asp Gln Met His Leu Asp Gly 355 360 365 Ile Ala Leu Val Asp Arg Arg Asn His Gln Gly Glu Gln Leu Ile Asp 370 375 380 Ala Asp Ser Glu Met Ala Ser Phe Ile Ala Arg Ser Val Asn Pro Leu 385 390 395 400 Cys Thr Val Ala His Phe Ser Gly Val Gly Leu Lys Leu Pro Pro Pro 405 410 415 Leu Gly Ala His Asn Asn Leu Lys Thr Leu Asn Leu Ser Ala Asn Ala 420 425 430 Ile Val Arg Met Leu Pro Gly Cys Leu Pro Lys Ser Leu His Thr Leu 435 440 445 Asp Leu Ser Arg Asn Lys Ile Val Val Ile Glu Gly Leu Arg Glu Leu 450 455 460 Ser Arg Leu Arg Val Leu Asn Leu Ser His Asn Arg Ile Ile Arg Ile 465 470 475 480 Gly His Gly Leu Ala Asn Cys Thr Ser Leu Arg Glu Ile Tyr Leu Ala 485 490 495 Gly Asn Lys Ile Ser Glu Ile Glu Gly Leu His Arg Leu Leu Lys Leu 500 505 510 Ser Phe Ile Asp Leu Ser Phe Asn Lys Ile Ala Ser Ala Lys Ser Ile 515 520 525 Gly Gln Leu Ala Ala Asn Tyr Asn Ser Leu Gln Ala Ile Asn Leu Leu 530 535 540 Gly Asn Pro Leu His Ser Asn Leu Gly Glu Glu Pro Leu Arg Lys Leu 545 550 555 560 Ile Val Gly Leu Thr Pro His Val Val Tyr Leu Asn Lys Gln Ala Thr 565 570 575 Lys Ala Val Ser Ala Arg Asp Ala Ser Val Asp Ser Val Ala Arg Ala 580 585 590 Ala Leu Ala Asn Pro Ser His His Thr His Gln Arg Gly Lys Thr Ser 595 600 605 Ser Gln Ser Lys Ser Phe Gly Glu Ala Val Arg Leu Pro His Pro Leu 610 615 620 Leu Pro Ala Pro Val Thr Gly Thr Asn Gly Leu Ala Arg Ser Leu Gln 625 630 635 640 Gln Val Val Pro Met His 645 7944DNAPhyscomitrella patensCDS(55)..(693) 7gcgatatcct cccttgcact gatccttact caccctcagt cgggtcagtg cgca atg 57 Met 1 gcc ggt gac gat gag aag gat gtg cgc gag gta gag gag gcc act tcc 105Ala Gly Asp Asp Glu Lys Asp Val Arg Glu Val Glu Glu Ala Thr Ser 5 10 15 tcg gga gcc act gcg gag gga tcc gac gaa gtc tcg aag gct ggt gag 153Ser Gly Ala Thr Ala Glu Gly Ser Asp Glu Val Ser Lys Ala Gly Glu 20 25 30 gaa gag gat act ggt gct cag atc gct cct atc gtg acg ctg cag gaa 201Glu Glu Asp Thr Gly Ala Gln Ile Ala Pro Ile Val Thr Leu Gln Glu 35 40 45 gtt gcc gtt atc acc ggc gag gag aat gag gac gtg cta att gat atg 249Val Ala Val Ile Thr Gly Glu Glu Asn Glu Asp Val Leu Ile Asp Met 50 55 60 65 aag gct aag ctg tat cga ttt gat aag gag gga aca cag tgg aaa gag 297Lys Ala Lys Leu Tyr Arg Phe Asp Lys Glu Gly Thr Gln Trp Lys Glu 70 75 80 aga ggt gtt ggt cag gtg aag atc ctg gag cac aag aca act gga aag 345Arg Gly Val Gly Gln Val Lys Ile Leu Glu His Lys Thr Thr Gly Lys 85 90 95 gtt cga ttg cta atg cga cag aac agg acc ctt aag atc tgt gcc aac 393Val Arg Leu Leu Met Arg Gln Asn Arg Thr Leu Lys Ile Cys Ala Asn 100 105 110 cac atg gtc tcg tca tct acg caa ctg caa gag cac gct ggt agc gat 441His Met Val Ser Ser Ser Thr Gln Leu Gln Glu His Ala Gly Ser Asp 115 120 125 aag act tgg gtc tgg cat gct cgg gat tac tca gat ggt gaa tta aaa 489Lys Thr Trp Val Trp His Ala Arg Asp Tyr Ser Asp Gly Glu Leu Lys 130 135 140 145 gag gag ctt ttc tgc atg cga ttt ggc agc gtt gaa agc gct caa aaa 537Glu Glu Leu Phe Cys Met Arg Phe Gly Ser Val Glu Ser Ala Gln Lys 150 155 160 ttc aag gat gtg tac gag gcc gcc caa gaa aag gca tcc agc aag aca 585Phe Lys Asp Val Tyr Glu Ala Ala Gln Glu Lys Ala Ser Ser Lys Thr 165 170 175 gag gag aag gac gaa gag gct gat gag gct gca gat ctt ttg gat aag 633Glu Glu Lys Asp Glu Glu Ala Asp Glu Ala Ala Asp Leu Leu Asp Lys 180 185 190 ttg aag gtg ggc tca aaa gcc gag aag gct gat gca cct gaa gag gcc 681Leu Lys Val Gly Ser Lys Ala Glu Lys Ala Asp Ala Pro Glu Glu Ala 195 200 205 aag act gaa aac taggggtgtg attaacatgt gcttgagttg atgttaggta 733Lys Thr Glu Asn 210 gtcgatcgtg ggttacccgg gctacatgat acagtgtttt gctaaccctt tagcagggtc 793ttagtgtggg atgtttccct ccaagttcaa tagctcaatc gtctcgacct gttgtttaga 853gttaattagt atgacatcag ttgcttttaa atgcctgttc tactacttct agcagcatgt 913attggatcac ttgctaggtg ctcgagctcg c 9448213PRTPhyscomitrella patens 8Met Ala Gly Asp Asp Glu Lys Asp Val Arg Glu Val Glu Glu Ala Thr 1 5 10 15 Ser Ser Gly Ala Thr Ala Glu Gly Ser Asp Glu Val Ser Lys Ala Gly 20 25 30 Glu Glu Glu Asp Thr Gly Ala Gln Ile Ala Pro Ile Val Thr Leu Gln 35 40 45 Glu Val Ala Val Ile Thr Gly Glu Glu Asn Glu Asp Val Leu Ile Asp 50 55 60 Met Lys Ala Lys Leu Tyr Arg Phe Asp Lys Glu Gly Thr Gln Trp Lys 65 70 75 80 Glu Arg Gly Val Gly Gln Val Lys Ile Leu Glu His Lys Thr Thr Gly 85 90 95 Lys Val Arg Leu Leu Met Arg Gln Asn Arg Thr Leu Lys Ile Cys Ala 100 105 110 Asn His Met Val Ser Ser Ser Thr Gln Leu Gln Glu His Ala Gly Ser 115 120 125 Asp Lys Thr Trp Val Trp His Ala Arg Asp Tyr Ser Asp Gly Glu Leu 130 135 140 Lys Glu Glu Leu Phe Cys Met Arg Phe Gly Ser Val Glu Ser Ala Gln 145 150 155 160 Lys Phe Lys Asp Val Tyr Glu Ala Ala Gln Glu Lys Ala Ser Ser Lys 165 170 175 Thr Glu Glu Lys Asp Glu Glu Ala Asp Glu Ala Ala Asp Leu Leu Asp 180 185 190 Lys Leu Lys Val Gly Ser Lys Ala Glu Lys Ala Asp Ala Pro Glu Glu 195 200 205 Ala Lys Thr Glu Asn 210 91772DNAPhyscomitrella patensCDS(47)..(1516) 9atggcgcgcc ttgtggacga ctgtgaatga gtcgaatcgc accatc atg atc acg 55 Met Ile Thr 1 tgt agg gtt tgg gtt ggt ttg ggg ccg gtg agc cct tct ttg att ctt 103Cys Arg Val Trp Val Gly Leu Gly Pro Val Ser Pro Ser Leu Ile Leu 5 10 15 ctg ccc tcg aag agt aac gga gaa tgc gtc cta agt gca aga aaa gct 151Leu Pro Ser Lys Ser Asn Gly Glu Cys Val Leu Ser Ala Arg Lys Ala 20 25 30 35 gat tgg gga tta ctg agc caa gtg caa tgc caa cgc ttt cga tgt cta 199Asp Trp Gly Leu Leu Ser Gln Val Gln Cys Gln Arg Phe Arg Cys Leu 40 45 50 tct tca gaa tat aag ggt cat aat ctt aaa ctt aga aga cgt agc cgt 247Ser Ser Glu Tyr Lys Gly His Asn Leu Lys Leu Arg Arg Arg Ser Arg 55 60 65 gtc tca gct tcc aac aga gaa aac ggt agt tta aat ggg cgt ttc cag 295Val Ser Ala Ser Asn Arg Glu Asn Gly Ser Leu Asn Gly Arg Phe Gln 70 75 80 gaa tca ctg agt caa gag aat ggg tat ccg gca cca act gaa ggg act 343Glu Ser Leu Ser Gln Glu Asn Gly Tyr Pro Ala Pro Thr Glu Gly Thr 85 90 95 gat cct cac act ttc tcc acg gcg atg gac tcc tta gct att aaa gca 391Asp Pro His Thr Phe Ser Thr Ala Met Asp Ser Leu Ala Ile Lys Ala 100 105 110 115 gag gaa gct tac aat gac gta cag gat tct ttt gcc aag agt agt aaa 439Glu Glu Ala Tyr Asn Asp Val Gln Asp Ser Phe Ala Lys Ser Ser Lys 120 125

130 caa cgg agc tta tct ggc tgc gct tct atc aaa gtg ttc ggt gtc ggg 487Gln Arg Ser Leu Ser Gly Cys Ala Ser Ile Lys Val Phe Gly Val Gly 135 140 145 ggt ggt gga tgc aat gcg gta gac gaa atg gtg agg tca gaa cta ttg 535Gly Gly Gly Cys Asn Ala Val Asp Glu Met Val Arg Ser Glu Leu Leu 150 155 160 aat gtt gag ttc tgg gcc gtc aat act gac aaa caa gca ttg aac aag 583Asn Val Glu Phe Trp Ala Val Asn Thr Asp Lys Gln Ala Leu Asn Lys 165 170 175 tcg ctg gct ccc aat aaa att caa att gga cag gac acg aca gcc ggc 631Ser Leu Ala Pro Asn Lys Ile Gln Ile Gly Gln Asp Thr Thr Ala Gly 180 185 190 195 cgc ggt gca ggt gga aga agt gca acc ggt gag gaa gca gct aca gag 679Arg Gly Ala Gly Gly Arg Ser Ala Thr Gly Glu Glu Ala Ala Thr Glu 200 205 210 tca ttg gcg gag ctt tcg atg gca ctt gaa ggt gcc gat tta gtc ttc 727Ser Leu Ala Glu Leu Ser Met Ala Leu Glu Gly Ala Asp Leu Val Phe 215 220 225 atc gcc tcc ggt atg ggt ggc ggt act ggt tca gga gca gct cct gtg 775Ile Ala Ser Gly Met Gly Gly Gly Thr Gly Ser Gly Ala Ala Pro Val 230 235 240 gtg gct cgg ttg gcg aag gct atg gga gcg tta acg att ggc ata gta 823Val Ala Arg Leu Ala Lys Ala Met Gly Ala Leu Thr Ile Gly Ile Val 245 250 255 act gaa cct ttc aca ttt gaa ggg ttc acc cga gct cga caa gct agg 871Thr Glu Pro Phe Thr Phe Glu Gly Phe Thr Arg Ala Arg Gln Ala Arg 260 265 270 275 aaa gcc att gag gac atg cgc cat gcg gct gac act gtg gtt gta gtt 919Lys Ala Ile Glu Asp Met Arg His Ala Ala Asp Thr Val Val Val Val 280 285 290 cca aat gat cgg ttg ctc cag act gta gca cct gac aca tct atg ctg 967Pro Asn Asp Arg Leu Leu Gln Thr Val Ala Pro Asp Thr Ser Met Leu 295 300 305 gag gct ttc cat ctt gca gat gac gtc ttg cgg cag gga gtg caa gga 1015Glu Ala Phe His Leu Ala Asp Asp Val Leu Arg Gln Gly Val Gln Gly 310 315 320 att tca gac atc atc acg ata ccc ggg cta gtc aac gtc gac ttt gcg 1063Ile Ser Asp Ile Ile Thr Ile Pro Gly Leu Val Asn Val Asp Phe Ala 325 330 335 gat gtg aaa gct atc atg tca aat gca ggg agt gca atg ttg gga atc 1111Asp Val Lys Ala Ile Met Ser Asn Ala Gly Ser Ala Met Leu Gly Ile 340 345 350 355 ggc gct ggt ttt ggg aag aac cgt gct gag gag gtg gca cgg tca gcc 1159Gly Ala Gly Phe Gly Lys Asn Arg Ala Glu Glu Val Ala Arg Ser Ala 360 365 370 atc atg tct cct cta ctc cgc tcc gtc tcg aga ccc atg ggt att gtg 1207Ile Met Ser Pro Leu Leu Arg Ser Val Ser Arg Pro Met Gly Ile Val 375 380 385 tac aat gtg aca ggt ggg agc gac cta act ctt cac gag gtc aac atc 1255Tyr Asn Val Thr Gly Gly Ser Asp Leu Thr Leu His Glu Val Asn Ile 390 395 400 gct gcc gaa att gtt cat gac atg gct gat cca aac gca aat gtt atc 1303Ala Ala Glu Ile Val His Asp Met Ala Asp Pro Asn Ala Asn Val Ile 405 410 415 ttt ggg gcg gtc att gat gag agc ttt aag ggg atg ata cgt atg act 1351Phe Gly Ala Val Ile Asp Glu Ser Phe Lys Gly Met Ile Arg Met Thr 420 425 430 435 gtc att gca act gga ttt aga gag cct gga gag gag aag gtc gtt ggt 1399Val Ile Ala Thr Gly Phe Arg Glu Pro Gly Glu Glu Lys Val Val Gly 440 445 450 agt gtt cga act gta gac gat gat ata ttc tac tgg gaa cag aat aag 1447Ser Val Arg Thr Val Asp Asp Asp Ile Phe Tyr Trp Glu Gln Asn Lys 455 460 465 aat agg tcc gac ctt ggc aaa gtg ccg gac gtt ttg cga aga aaa gat 1495Asn Arg Ser Asp Leu Gly Lys Val Pro Asp Val Leu Arg Arg Lys Asp 470 475 480 cga agg cgt ggc agt ggc agg taactgccgg gtttactctt tatgcgtatg 1546Arg Arg Arg Gly Ser Gly Arg 485 490 ggatttaaag agaagccgct gagcctgaga ttctacagga ggtttgtgga gttgttgtga 1606tcaagcacca tctttcaata gaatgaagat catggtttta aaaacagtga gcatttctca 1666atcgatactc tgaaagcctg tactcaaagc tggagctgcg aaaacagtgt acgaagggca 1726ctgatttgat gacacagcat ctttgtgaat agaaccagga tatcgc 177210490PRTPhyscomitrella patens 10Met Ile Thr Cys Arg Val Trp Val Gly Leu Gly Pro Val Ser Pro Ser 1 5 10 15 Leu Ile Leu Leu Pro Ser Lys Ser Asn Gly Glu Cys Val Leu Ser Ala 20 25 30 Arg Lys Ala Asp Trp Gly Leu Leu Ser Gln Val Gln Cys Gln Arg Phe 35 40 45 Arg Cys Leu Ser Ser Glu Tyr Lys Gly His Asn Leu Lys Leu Arg Arg 50 55 60 Arg Ser Arg Val Ser Ala Ser Asn Arg Glu Asn Gly Ser Leu Asn Gly 65 70 75 80 Arg Phe Gln Glu Ser Leu Ser Gln Glu Asn Gly Tyr Pro Ala Pro Thr 85 90 95 Glu Gly Thr Asp Pro His Thr Phe Ser Thr Ala Met Asp Ser Leu Ala 100 105 110 Ile Lys Ala Glu Glu Ala Tyr Asn Asp Val Gln Asp Ser Phe Ala Lys 115 120 125 Ser Ser Lys Gln Arg Ser Leu Ser Gly Cys Ala Ser Ile Lys Val Phe 130 135 140 Gly Val Gly Gly Gly Gly Cys Asn Ala Val Asp Glu Met Val Arg Ser 145 150 155 160 Glu Leu Leu Asn Val Glu Phe Trp Ala Val Asn Thr Asp Lys Gln Ala 165 170 175 Leu Asn Lys Ser Leu Ala Pro Asn Lys Ile Gln Ile Gly Gln Asp Thr 180 185 190 Thr Ala Gly Arg Gly Ala Gly Gly Arg Ser Ala Thr Gly Glu Glu Ala 195 200 205 Ala Thr Glu Ser Leu Ala Glu Leu Ser Met Ala Leu Glu Gly Ala Asp 210 215 220 Leu Val Phe Ile Ala Ser Gly Met Gly Gly Gly Thr Gly Ser Gly Ala 225 230 235 240 Ala Pro Val Val Ala Arg Leu Ala Lys Ala Met Gly Ala Leu Thr Ile 245 250 255 Gly Ile Val Thr Glu Pro Phe Thr Phe Glu Gly Phe Thr Arg Ala Arg 260 265 270 Gln Ala Arg Lys Ala Ile Glu Asp Met Arg His Ala Ala Asp Thr Val 275 280 285 Val Val Val Pro Asn Asp Arg Leu Leu Gln Thr Val Ala Pro Asp Thr 290 295 300 Ser Met Leu Glu Ala Phe His Leu Ala Asp Asp Val Leu Arg Gln Gly 305 310 315 320 Val Gln Gly Ile Ser Asp Ile Ile Thr Ile Pro Gly Leu Val Asn Val 325 330 335 Asp Phe Ala Asp Val Lys Ala Ile Met Ser Asn Ala Gly Ser Ala Met 340 345 350 Leu Gly Ile Gly Ala Gly Phe Gly Lys Asn Arg Ala Glu Glu Val Ala 355 360 365 Arg Ser Ala Ile Met Ser Pro Leu Leu Arg Ser Val Ser Arg Pro Met 370 375 380 Gly Ile Val Tyr Asn Val Thr Gly Gly Ser Asp Leu Thr Leu His Glu 385 390 395 400 Val Asn Ile Ala Ala Glu Ile Val His Asp Met Ala Asp Pro Asn Ala 405 410 415 Asn Val Ile Phe Gly Ala Val Ile Asp Glu Ser Phe Lys Gly Met Ile 420 425 430 Arg Met Thr Val Ile Ala Thr Gly Phe Arg Glu Pro Gly Glu Glu Lys 435 440 445 Val Val Gly Ser Val Arg Thr Val Asp Asp Asp Ile Phe Tyr Trp Glu 450 455 460 Gln Asn Lys Asn Arg Ser Asp Leu Gly Lys Val Pro Asp Val Leu Arg 465 470 475 480 Arg Lys Asp Arg Arg Arg Gly Ser Gly Arg 485 490 111446DNAPhyscomitrella patensCDS(453)..(1343) 11atcccgggca gtgaagtccg ggtttgggtg cttcttaact tttcttctct tttggagttg 60ggaagatttt tttttgcgga ggatgttttg ggagggttgg ggttgaagtt gtcttggtga 120ttgaggctgt ggcggaggag tattgatcga agtttggtgt tcaaggaggt gttgcctttc 180atggtcagaa acgatcttcg tcgccgctct gcgtgcagtc ttgcagcagt tgctggtttt 240cacgatcggg aggttcgtgt ggagcgcggg gtggacttgc tgctcgtttc tggtgtgcgt 300ttgctgcacg actgttgttc cctttcctgg gaattcaaca ttgattgatt gacgagcgac 360ttcgcgatcg gcattggtat ttgccgcgtc gtgagctttg cgacgttttc agatatggag 420tggaaggggt ttgctgaggt ggtttggcgt cg atg atc gcc ggg ttc gct acg 473 Met Ile Ala Gly Phe Ala Thr 1 5 cac cct ctg gac ctt atc aag gtc cgc atg cag tta caa ggg gag gtt 521His Pro Leu Asp Leu Ile Lys Val Arg Met Gln Leu Gln Gly Glu Val 10 15 20 gct acg tcg ggt ttc gcc ctc gcg ctc gaa ggt agt cat gtt gct cct 569Ala Thr Ser Gly Phe Ala Leu Ala Leu Glu Gly Ser His Val Ala Pro 25 30 35 gct gta ctc ggt gtc ccg aaa ccg ggt ccc ttg gga gtc ggt ttg aat 617Ala Val Leu Gly Val Pro Lys Pro Gly Pro Leu Gly Val Gly Leu Asn 40 45 50 55 gtg gct cgt gca gaa gga gtg tat gcc ctc tac tcc ggt gtc tcc gcc 665Val Ala Arg Ala Glu Gly Val Tyr Ala Leu Tyr Ser Gly Val Ser Ala 60 65 70 act ttg tta aga caa gcc atg tat tcg tct aca cgg atg ggt ctt tac 713Thr Leu Leu Arg Gln Ala Met Tyr Ser Ser Thr Arg Met Gly Leu Tyr 75 80 85 gag ttc ttg aag cat cag tgg aga gac gag aaa caa gaa ggc tct ggg 761Glu Phe Leu Lys His Gln Trp Arg Asp Glu Lys Gln Glu Gly Ser Gly 90 95 100 ctt cct ctg tac aaa aaa gtg acc gct gca ttg att gcc ggg gct tcc 809Leu Pro Leu Tyr Lys Lys Val Thr Ala Ala Leu Ile Ala Gly Ala Ser 105 110 115 ggc gcc gtt gtt gga aac cct gca gac ttg gcc atg gtc agg atg caa 857Gly Ala Val Val Gly Asn Pro Ala Asp Leu Ala Met Val Arg Met Gln 120 125 130 135 gcc gac ggt agg ctg cct atg cat gag agg agg aac tac acc ggg gtc 905Ala Asp Gly Arg Leu Pro Met His Glu Arg Arg Asn Tyr Thr Gly Val 140 145 150 ggc aat gct ctg tta cgg atg gtg aaa caa gac ggc gtg atg tca ttg 953Gly Asn Ala Leu Leu Arg Met Val Lys Gln Asp Gly Val Met Ser Leu 155 160 165 tgg acg gga tcg gct ccg act gtg act cga gcc atg ctg gtg acc gcc 1001Trp Thr Gly Ser Ala Pro Thr Val Thr Arg Ala Met Leu Val Thr Ala 170 175 180 gct cag ttg gcc acc tac gac cag atc aag gac tcc att gct gag acc 1049Ala Gln Leu Ala Thr Tyr Asp Gln Ile Lys Asp Ser Ile Ala Glu Thr 185 190 195 cac atg gtg ccg gaa ggg ctg gcc acg cag gtg gtg gca agc tgc gga 1097His Met Val Pro Glu Gly Leu Ala Thr Gln Val Val Ala Ser Cys Gly 200 205 210 215 gcg ggg gtg ctg gca tcc gtc gct tca aac ccc atc gac gtc gtg aag 1145Ala Gly Val Leu Ala Ser Val Ala Ser Asn Pro Ile Asp Val Val Lys 220 225 230 acg aga gtg atg aac atg aaa gtg acg cct gga gaa gga gct cct tat 1193Thr Arg Val Met Asn Met Lys Val Thr Pro Gly Glu Gly Ala Pro Tyr 235 240 245 cga ggt gct ttg gat tgt gct gtg aag acg gtg cga gcg gaa ggt ccc 1241Arg Gly Ala Leu Asp Cys Ala Val Lys Thr Val Arg Ala Glu Gly Pro 250 255 260 atg gct ctg tac aag gga ttt gtc ccg acg gtg act cgt caa ggc ccc 1289Met Ala Leu Tyr Lys Gly Phe Val Pro Thr Val Thr Arg Gln Gly Pro 265 270 275 ttc gcc ata gtt ctg ttc ctg tca ttg gag cag atc aag aag ctg atc 1337Phe Ala Ile Val Leu Phe Leu Ser Leu Glu Gln Ile Lys Lys Leu Ile 280 285 290 295 gag ggc tgaatcagat aatgacgaaa gatgtgtagt taagcaatag ttgaagtgga 1393Glu Gly tataataagg tccatatctg aagaggttgt ctcggagaat tgggcgttaa cgc 144612297PRTPhyscomitrella patens 12Met Ile Ala Gly Phe Ala Thr His Pro Leu Asp Leu Ile Lys Val Arg 1 5 10 15 Met Gln Leu Gln Gly Glu Val Ala Thr Ser Gly Phe Ala Leu Ala Leu 20 25 30 Glu Gly Ser His Val Ala Pro Ala Val Leu Gly Val Pro Lys Pro Gly 35 40 45 Pro Leu Gly Val Gly Leu Asn Val Ala Arg Ala Glu Gly Val Tyr Ala 50 55 60 Leu Tyr Ser Gly Val Ser Ala Thr Leu Leu Arg Gln Ala Met Tyr Ser 65 70 75 80 Ser Thr Arg Met Gly Leu Tyr Glu Phe Leu Lys His Gln Trp Arg Asp 85 90 95 Glu Lys Gln Glu Gly Ser Gly Leu Pro Leu Tyr Lys Lys Val Thr Ala 100 105 110 Ala Leu Ile Ala Gly Ala Ser Gly Ala Val Val Gly Asn Pro Ala Asp 115 120 125 Leu Ala Met Val Arg Met Gln Ala Asp Gly Arg Leu Pro Met His Glu 130 135 140 Arg Arg Asn Tyr Thr Gly Val Gly Asn Ala Leu Leu Arg Met Val Lys 145 150 155 160 Gln Asp Gly Val Met Ser Leu Trp Thr Gly Ser Ala Pro Thr Val Thr 165 170 175 Arg Ala Met Leu Val Thr Ala Ala Gln Leu Ala Thr Tyr Asp Gln Ile 180 185 190 Lys Asp Ser Ile Ala Glu Thr His Met Val Pro Glu Gly Leu Ala Thr 195 200 205 Gln Val Val Ala Ser Cys Gly Ala Gly Val Leu Ala Ser Val Ala Ser 210 215 220 Asn Pro Ile Asp Val Val Lys Thr Arg Val Met Asn Met Lys Val Thr 225 230 235 240 Pro Gly Glu Gly Ala Pro Tyr Arg Gly Ala Leu Asp Cys Ala Val Lys 245 250 255 Thr Val Arg Ala Glu Gly Pro Met Ala Leu Tyr Lys Gly Phe Val Pro 260 265 270 Thr Val Thr Arg Gln Gly Pro Phe Ala Ile Val Leu Phe Leu Ser Leu 275 280 285 Glu Gln Ile Lys Lys Leu Ile Glu Gly 290 295 13937DNAPhyscomitrella patensCDS(33)..(593) 13atcccgggtc gacacggcgg aagggtgggg tt atg ggt cgc ggc aaa att gag 53 Met Gly Arg Gly Lys Ile Glu 1 5 atc aag aag att gag aat aca acc agc agg cag gtg aca ttc tcc aag 101Ile Lys Lys Ile Glu Asn Thr Thr Ser Arg Gln Val Thr Phe Ser Lys 10 15 20 agg cgc ggt ggt ctt ttg aag aag gcg cac gag ctt gcg gtt ctg tgt 149Arg Arg Gly Gly Leu Leu Lys Lys Ala His Glu Leu Ala Val Leu Cys 25 30 35 gat gcc gag gtg gcg ctg gtt att ttc tcc agc act gga aag cac ttt 197Asp Ala Glu Val Ala Leu Val Ile Phe Ser Ser Thr Gly Lys His Phe 40 45 50 55 gag ttt gcc agt tca ggc agc atg cgg gac atc att gag cgg tac agg 245Glu Phe Ala Ser Ser Gly Ser Met Arg Asp Ile Ile Glu Arg Tyr Arg 60 65 70 aag agc tcg gat ggt gca gtg aag cgt ggc acc aat act gat tta ctt 293Lys Ser Ser Asp Gly Ala Val Lys Arg Gly Thr Asn Thr Asp Leu Leu 75 80 85 ggt cgg gag gtg att aag tta aaa cag caa gta gaa cga ttg gaa agc 341Gly Arg Glu Val Ile Lys Leu Lys Gln Gln Val Glu Arg Leu Glu Ser 90 95 100 tct caa agg cat atg ctt ggt gag gat ctt tca gct ttg aag gta tct 389Ser Gln Arg His Met Leu

Gly Glu Asp Leu Ser Ala Leu Lys Val Ser 105 110 115 gac ctt ttg gag ctg gag cag cag ctt gat cag ggt gct tca cga gtg 437Asp Leu Leu Glu Leu Glu Gln Gln Leu Asp Gln Gly Ala Ser Arg Val 120 125 130 135 aga gca agg aag aat caa ctc att tta gaa gag atc gaa gac ttg cgg 485Arg Ala Arg Lys Asn Gln Leu Ile Leu Glu Glu Ile Glu Asp Leu Arg 140 145 150 aga aag gag cat gaa ctg atg att gca aac gag gct ctt cgc aag aag 533Arg Lys Glu His Glu Leu Met Ile Ala Asn Glu Ala Leu Arg Lys Lys 155 160 165 att gca gac gct gaa ggt gct gcg gaa gca gag ctc gag cta att tcc 581Ile Ala Asp Ala Glu Gly Ala Ala Glu Ala Glu Leu Glu Leu Ile Ser 170 175 180 cgg atg ctc ggc tagaaagccc caaaccgttc gccagcgatt tctcacgaga 633Arg Met Leu Gly 185 tatgagtgtg agttcgcagc tggcagcatc agtctaccct catcccaacc ttctacaggc 693gcaaagatca cagacgtcat tacagcttgg atggctatct gagcaacaga tccccagcac 753ggaagaaggt tgtgcaggtg aatctagctt gaaatgggat catccgcact ttcacattca 813gaatagactc catgcaaaca tatcaccaag tgtgagaatt tataagttac atgtcgatcg 873ataagccact tcgaacgaca gaaggctctt tgggagccca ggctatgggt cctagcgtta 933acgc 93714187PRTPhyscomitrella patens 14Met Gly Arg Gly Lys Ile Glu Ile Lys Lys Ile Glu Asn Thr Thr Ser 1 5 10 15 Arg Gln Val Thr Phe Ser Lys Arg Arg Gly Gly Leu Leu Lys Lys Ala 20 25 30 His Glu Leu Ala Val Leu Cys Asp Ala Glu Val Ala Leu Val Ile Phe 35 40 45 Ser Ser Thr Gly Lys His Phe Glu Phe Ala Ser Ser Gly Ser Met Arg 50 55 60 Asp Ile Ile Glu Arg Tyr Arg Lys Ser Ser Asp Gly Ala Val Lys Arg 65 70 75 80 Gly Thr Asn Thr Asp Leu Leu Gly Arg Glu Val Ile Lys Leu Lys Gln 85 90 95 Gln Val Glu Arg Leu Glu Ser Ser Gln Arg His Met Leu Gly Glu Asp 100 105 110 Leu Ser Ala Leu Lys Val Ser Asp Leu Leu Glu Leu Glu Gln Gln Leu 115 120 125 Asp Gln Gly Ala Ser Arg Val Arg Ala Arg Lys Asn Gln Leu Ile Leu 130 135 140 Glu Glu Ile Glu Asp Leu Arg Arg Lys Glu His Glu Leu Met Ile Ala 145 150 155 160 Asn Glu Ala Leu Arg Lys Lys Ile Ala Asp Ala Glu Gly Ala Ala Glu 165 170 175 Ala Glu Leu Glu Leu Ile Ser Arg Met Leu Gly 180 185 151082DNAPhyscomitrella patensCDS(25)..(1053) 15atcccgggca ttctctctct cgca atg gcg tcc gag ggt gtg ctt ttg ggc 51 Met Ala Ser Glu Gly Val Leu Leu Gly 1 5 atg gga aac ccc ctg ctc gac atc tcc tgc gtg gtc gac gac gca ttc 99Met Gly Asn Pro Leu Leu Asp Ile Ser Cys Val Val Asp Asp Ala Phe 10 15 20 25 ctc gag aag tac ggg ctg acg cta aac aac gct att ctt gct gag gac 147Leu Glu Lys Tyr Gly Leu Thr Leu Asn Asn Ala Ile Leu Ala Glu Asp 30 35 40 aag cac ctt ccc atg tac aag gaa ctg gct gcc aat ccc gat gta gag 195Lys His Leu Pro Met Tyr Lys Glu Leu Ala Ala Asn Pro Asp Val Glu 45 50 55 tac att gca gga ggt gct act cag aac acc atc agg att gcc cag tgg 243Tyr Ile Ala Gly Gly Ala Thr Gln Asn Thr Ile Arg Ile Ala Gln Trp 60 65 70 atg cta ggt gaa tcg aac gca act agc tac ttt ggc tgt gtt ggc aag 291Met Leu Gly Glu Ser Asn Ala Thr Ser Tyr Phe Gly Cys Val Gly Lys 75 80 85 gat gag tat ggc gac cgt atg ttc aag ctc gcc tct gag gga ggt gtc 339Asp Glu Tyr Gly Asp Arg Met Phe Lys Leu Ala Ser Glu Gly Gly Val 90 95 100 105 aat atc cga tac gat gtg gac gag gat ctt ccc act gga aca tgc ggc 387Asn Ile Arg Tyr Asp Val Asp Glu Asp Leu Pro Thr Gly Thr Cys Gly 110 115 120 gtg ctc gtg gtg aag gga gag agg tcc ttg gta gcc aat ctt tca gcc 435Val Leu Val Val Lys Gly Glu Arg Ser Leu Val Ala Asn Leu Ser Ala 125 130 135 gcc aac aaa tac aag atc gac cac ttg aag aag cca gaa aac tgg gct 483Ala Asn Lys Tyr Lys Ile Asp His Leu Lys Lys Pro Glu Asn Trp Ala 140 145 150 ttc gtg gag aag gca aag tac atc tac agc gcc ggt ttc ttc ctg act 531Phe Val Glu Lys Ala Lys Tyr Ile Tyr Ser Ala Gly Phe Phe Leu Thr 155 160 165 gtt tca ccg gaa tct atg atg acc gtg gcc aaa cat gct gcc gag acc 579Val Ser Pro Glu Ser Met Met Thr Val Ala Lys His Ala Ala Glu Thr 170 175 180 185 gga aaa tac tac atg atc aac tta gcc gct ccg ttc atc tgc cag ttc 627Gly Lys Tyr Tyr Met Ile Asn Leu Ala Ala Pro Phe Ile Cys Gln Phe 190 195 200 ttt aag gac cct ctt atg gag ctt ttc cct tac gtg gat ttc att ttc 675Phe Lys Asp Pro Leu Met Glu Leu Phe Pro Tyr Val Asp Phe Ile Phe 205 210 215 ggc aac gag agc gag gcc aga gca ttt gcg caa gtt caa ggc tgg gag 723Gly Asn Glu Ser Glu Ala Arg Ala Phe Ala Gln Val Gln Gly Trp Glu 220 225 230 aca gag gac acc aag gtg ata gcc gta aag ttg gct gcg tta ccg aaa 771Thr Glu Asp Thr Lys Val Ile Ala Val Lys Leu Ala Ala Leu Pro Lys 235 240 245 gct ggc ggc acc cac aag cgt gtc gct gtc atc acc cag gga act gac 819Ala Gly Gly Thr His Lys Arg Val Ala Val Ile Thr Gln Gly Thr Asp 250 255 260 265 ccc aca att gtt gct gaa gat gga aag gtg act gaa ttc ccc gtc acc 867Pro Thr Ile Val Ala Glu Asp Gly Lys Val Thr Glu Phe Pro Val Thr 270 275 280 cct att cct aag gag aag ttg gtc gac act aat gca gct ggt gac tct 915Pro Ile Pro Lys Glu Lys Leu Val Asp Thr Asn Ala Ala Gly Asp Ser 285 290 295 ttt gtc gga ggg ttc ttg tct cag ctg gtg ttg ggt aaa gac atc gca 963Phe Val Gly Gly Phe Leu Ser Gln Leu Val Leu Gly Lys Asp Ile Ala 300 305 310 cag tgc gtc aga gca gga aac tac gca gcc agc gtc atc atc cag cgc 1011Gln Cys Val Arg Ala Gly Asn Tyr Ala Ala Ser Val Ile Ile Gln Arg 315 320 325 tct gga tgc act ttc cct tcc aaa cca tcc ttc gaa agt cag 1053Ser Gly Cys Thr Phe Pro Ser Lys Pro Ser Phe Glu Ser Gln 330 335 340 tagaaaattg ttagggtagg ggagctcgc 108216343PRTPhyscomitrella patens 16Met Ala Ser Glu Gly Val Leu Leu Gly Met Gly Asn Pro Leu Leu Asp 1 5 10 15 Ile Ser Cys Val Val Asp Asp Ala Phe Leu Glu Lys Tyr Gly Leu Thr 20 25 30 Leu Asn Asn Ala Ile Leu Ala Glu Asp Lys His Leu Pro Met Tyr Lys 35 40 45 Glu Leu Ala Ala Asn Pro Asp Val Glu Tyr Ile Ala Gly Gly Ala Thr 50 55 60 Gln Asn Thr Ile Arg Ile Ala Gln Trp Met Leu Gly Glu Ser Asn Ala 65 70 75 80 Thr Ser Tyr Phe Gly Cys Val Gly Lys Asp Glu Tyr Gly Asp Arg Met 85 90 95 Phe Lys Leu Ala Ser Glu Gly Gly Val Asn Ile Arg Tyr Asp Val Asp 100 105 110 Glu Asp Leu Pro Thr Gly Thr Cys Gly Val Leu Val Val Lys Gly Glu 115 120 125 Arg Ser Leu Val Ala Asn Leu Ser Ala Ala Asn Lys Tyr Lys Ile Asp 130 135 140 His Leu Lys Lys Pro Glu Asn Trp Ala Phe Val Glu Lys Ala Lys Tyr 145 150 155 160 Ile Tyr Ser Ala Gly Phe Phe Leu Thr Val Ser Pro Glu Ser Met Met 165 170 175 Thr Val Ala Lys His Ala Ala Glu Thr Gly Lys Tyr Tyr Met Ile Asn 180 185 190 Leu Ala Ala Pro Phe Ile Cys Gln Phe Phe Lys Asp Pro Leu Met Glu 195 200 205 Leu Phe Pro Tyr Val Asp Phe Ile Phe Gly Asn Glu Ser Glu Ala Arg 210 215 220 Ala Phe Ala Gln Val Gln Gly Trp Glu Thr Glu Asp Thr Lys Val Ile 225 230 235 240 Ala Val Lys Leu Ala Ala Leu Pro Lys Ala Gly Gly Thr His Lys Arg 245 250 255 Val Ala Val Ile Thr Gln Gly Thr Asp Pro Thr Ile Val Ala Glu Asp 260 265 270 Gly Lys Val Thr Glu Phe Pro Val Thr Pro Ile Pro Lys Glu Lys Leu 275 280 285 Val Asp Thr Asn Ala Ala Gly Asp Ser Phe Val Gly Gly Phe Leu Ser 290 295 300 Gln Leu Val Leu Gly Lys Asp Ile Ala Gln Cys Val Arg Ala Gly Asn 305 310 315 320 Tyr Ala Ala Ser Val Ile Ile Gln Arg Ser Gly Cys Thr Phe Pro Ser 325 330 335 Lys Pro Ser Phe Glu Ser Gln 340 172357DNAPhyscomitrella patensCDS(126)..(1907) 17atcccgggag tggtggtgat ggtattggtc atcgtggtcg cgtaaaagaa gaagacaagg 60tgacaatttt ggtcctcctc ccttccttgc tactcctcga agagtatttc ctactgagtt 120gagac atg gag tct gag gac gaa atg caa gat gca tgg gct ggt aca tca 170 Met Glu Ser Glu Asp Glu Met Gln Asp Ala Trp Ala Gly Thr Ser 1 5 10 15 gat ggt gag ttt gta aac gaa gag gaa gat gag gaa tca gat gct tta 218Asp Gly Glu Phe Val Asn Glu Glu Glu Asp Glu Glu Ser Asp Ala Leu 20 25 30 gct agt gat gac gac aat gat gaa tcg gat tat ggt ttt gat tac tcg 266Ala Ser Asp Asp Asp Asn Asp Glu Ser Asp Tyr Gly Phe Asp Tyr Ser 35 40 45 aac gtg gac gac ctt cac cct agt tct cgg cta ccc cag act aac ttc 314Asn Val Asp Asp Leu His Pro Ser Ser Arg Leu Pro Gln Thr Asn Phe 50 55 60 aca atc ctt agt gag aaa gat ata cgg caa cga caa gac gaa gcc gta 362Thr Ile Leu Ser Glu Lys Asp Ile Arg Gln Arg Gln Asp Glu Ala Val 65 70 75 tca act atc act aat ttc ttg tct ata tct cca gcg gat gcc ggg gtt 410Ser Thr Ile Thr Asn Phe Leu Ser Ile Ser Pro Ala Asp Ala Gly Val 80 85 90 95 ctg ctt cgg cac ttt aag tgg agt gtg agt aaa gtg aat gac gag tgg 458Leu Leu Arg His Phe Lys Trp Ser Val Ser Lys Val Asn Asp Glu Trp 100 105 110 ttt gcg gat gag gaa cga gtg cgc gca agc gtc ggt tta ctg gag aaa 506Phe Ala Asp Glu Glu Arg Val Arg Ala Ser Val Gly Leu Leu Glu Lys 115 120 125 ccc gct acc agc aaa aga caa act caa aca gag atg act tgc gag ata 554Pro Ala Thr Ser Lys Arg Gln Thr Gln Thr Glu Met Thr Cys Glu Ile 130 135 140 tgt ttt gag gtg cat ccg ttt gaa aaa atg agg gca cct aga tgc ggt 602Cys Phe Glu Val His Pro Phe Glu Lys Met Arg Ala Pro Arg Cys Gly 145 150 155 cac tat ttc tgt gag acc tgt tgg aca ggt tac ata cac aca gct att 650His Tyr Phe Cys Glu Thr Cys Trp Thr Gly Tyr Ile His Thr Ala Ile 160 165 170 175 aat gat ggg cct gga tgt ttg act ctt cga tgt gcg gat cca tcc tgt 698Asn Asp Gly Pro Gly Cys Leu Thr Leu Arg Cys Ala Asp Pro Ser Cys 180 185 190 ggc tca gcc att gga gaa gat atg gta cta agt tta gtc tcg acg gat 746Gly Ser Ala Ile Gly Glu Asp Met Val Leu Ser Leu Val Ser Thr Asp 195 200 205 gat caa cag aag tac atg cgt tat cta tta aga tct tac gtg gag gac 794Asp Gln Gln Lys Tyr Met Arg Tyr Leu Leu Arg Ser Tyr Val Glu Asp 210 215 220 aac cga aag gtc aag tgg tgc cca gca cct ggc tgt gaa tat gct gtg 842Asn Arg Lys Val Lys Trp Cys Pro Ala Pro Gly Cys Glu Tyr Ala Val 225 230 235 gaa ttt caa cct ggc gtt ggt tcc tat gac ctt gtc tgc aag tgt gga 890Glu Phe Gln Pro Gly Val Gly Ser Tyr Asp Leu Val Cys Lys Cys Gly 240 245 250 255 ttt aac ttc tgc tgg aat tgt cga gaa gag gca cac cgg ccc gtg gat 938Phe Asn Phe Cys Trp Asn Cys Arg Glu Glu Ala His Arg Pro Val Asp 260 265 270 tgt gag aca gtc aat aaa tgg ata ttg aaa aat tgt gca gag tcg gag 986Cys Glu Thr Val Asn Lys Trp Ile Leu Lys Asn Cys Ala Glu Ser Glu 275 280 285 aac atg aac tgg att ctt gcg aac agc aaa ccc tgt ccc aaa tgt aaa 1034Asn Met Asn Trp Ile Leu Ala Asn Ser Lys Pro Cys Pro Lys Cys Lys 290 295 300 agg cca atc gaa aag aat caa ggc tgc atg cac att act tgc aca cca 1082Arg Pro Ile Glu Lys Asn Gln Gly Cys Met His Ile Thr Cys Thr Pro 305 310 315 cct tgc aaa ttt gag ttt tgc tgg ctt tgc ctt gga gca tgg act gat 1130Pro Cys Lys Phe Glu Phe Cys Trp Leu Cys Leu Gly Ala Trp Thr Asp 320 325 330 335 cat ggg gaa agg acg ggt ggt ttc tat gca tgc aat cgg tat gaa acc 1178His Gly Glu Arg Thr Gly Gly Phe Tyr Ala Cys Asn Arg Tyr Glu Thr 340 345 350 gcg aag caa gaa ggc gtg tat gat gaa gca gag cgg aga agg gaa atg 1226Ala Lys Gln Glu Gly Val Tyr Asp Glu Ala Glu Arg Arg Arg Glu Met 355 360 365 gcg aag aac tcc ctt gaa cgt tac aca cac tat tat gag cgt tgg gct 1274Ala Lys Asn Ser Leu Glu Arg Tyr Thr His Tyr Tyr Glu Arg Trp Ala 370 375 380 aca aac gaa tct tcc agg gca aag gcg ctt gca gat ctt caa gat atg 1322Thr Asn Glu Ser Ser Arg Ala Lys Ala Leu Ala Asp Leu Gln Asp Met 385 390 395 cag aac gtg cag att gaa aag ctg agc gtc act caa tgc caa cca gtg 1370Gln Asn Val Gln Ile Glu Lys Leu Ser Val Thr Gln Cys Gln Pro Val 400 405 410 415 tcg cag ctg aaa ttt gta aca gat gca tgg ctc cag att gta gaa tgc 1418Ser Gln Leu Lys Phe Val Thr Asp Ala Trp Leu Gln Ile Val Glu Cys 420 425 430 cgg cgt gta ttg aag tgg aca tat gct tat gga tat tac tta cct gag 1466Arg Arg Val Leu Lys Trp Thr Tyr Ala Tyr Gly Tyr Tyr Leu Pro Glu 435 440 445 aat gag cac acg aaa aga caa ttc ttt gaa tac tcg caa ggt gag gcc 1514Asn Glu His Thr Lys Arg Gln Phe Phe Glu Tyr Ser Gln Gly Glu Ala 450 455 460 gaa gct ggt tta gag cgc ctc cac cag tgt gcg gag aag gat ttg cta 1562Glu Ala Gly Leu Glu Arg Leu His Gln Cys Ala Glu Lys Asp Leu Leu 465 470 475 acc ttt ctt gga ggc act cca acc agc tca ttc aat gac ttc cgc acc 1610Thr Phe Leu Gly Gly Thr Pro Thr Ser Ser Phe Asn Asp Phe Arg Thr 480 485 490 495 aag ctt gct ggc ctg acc agt gtg aca aag aca tac ttc gag aac ctg 1658Lys Leu Ala Gly Leu Thr Ser Val Thr Lys Thr Tyr Phe Glu Asn Leu 500 505 510 gtt cgc gcc cta gag aac aat ctt tcg gac gtg gat att cca aaa gca 1706Val Arg Ala Leu Glu Asn Asn Leu Ser Asp Val Asp Ile Pro Lys Ala 515 520

525 gct gct aag tcc agc agc agc tcg aag gct tca gga agt tcc aaa ggg 1754Ala Ala Lys Ser Ser Ser Ser Ser Lys Ala Ser Gly Ser Ser Lys Gly 530 535 540 cga ggg ggc agg cct aaa gtg gga agt tcc aaa agt ggg ggt tct agt 1802Arg Gly Gly Arg Pro Lys Val Gly Ser Ser Lys Ser Gly Gly Ser Ser 545 550 555 cgg agc gga gag gag tca act cac tgg tct tgt gag cat tgc acg tac 1850Arg Ser Gly Glu Glu Ser Thr His Trp Ser Cys Glu His Cys Thr Tyr 560 565 570 575 gcc aat acc aca gcc gcg tct tcc att gtg tgc gtc ata tgc aat cac 1898Ala Asn Thr Thr Ala Ala Ser Ser Ile Val Cys Val Ile Cys Asn His 580 585 590 gct cga tcg tgatgtaaat tctcgcttca gtatctcttt cccgactgag 1947Ala Arg Ser cttgtcagtt ctcctaacac tccagatcct tcaaatttgc tatgtcattg ttgatccagt 2007ccaggatcaa agaccactgt attcagatat ggtggttgaa ggcatttctg ctttgtttaa 2067ggctattcat agaagttaat cacaatgttg acagttaaat ccttgcgtca ggcatctggg 2127tattgttgat atcagttgtc catcccctaa attttttgtt gtatacaatc tagccaacaa 2187agtgggtgaa aatgaagaac ggagggattt tatgtatcga gaccttgtgg tgcacaattt 2247gcaacggcag tctatctagt tgcgatgtac cacatctgtc tctatctact gtaaatgtgg 2307tagttgtaaa agcgtactcc aaaacagaat tccccctcgt gcgagctcgc 235718594PRTPhyscomitrella patens 18Met Glu Ser Glu Asp Glu Met Gln Asp Ala Trp Ala Gly Thr Ser Asp 1 5 10 15 Gly Glu Phe Val Asn Glu Glu Glu Asp Glu Glu Ser Asp Ala Leu Ala 20 25 30 Ser Asp Asp Asp Asn Asp Glu Ser Asp Tyr Gly Phe Asp Tyr Ser Asn 35 40 45 Val Asp Asp Leu His Pro Ser Ser Arg Leu Pro Gln Thr Asn Phe Thr 50 55 60 Ile Leu Ser Glu Lys Asp Ile Arg Gln Arg Gln Asp Glu Ala Val Ser 65 70 75 80 Thr Ile Thr Asn Phe Leu Ser Ile Ser Pro Ala Asp Ala Gly Val Leu 85 90 95 Leu Arg His Phe Lys Trp Ser Val Ser Lys Val Asn Asp Glu Trp Phe 100 105 110 Ala Asp Glu Glu Arg Val Arg Ala Ser Val Gly Leu Leu Glu Lys Pro 115 120 125 Ala Thr Ser Lys Arg Gln Thr Gln Thr Glu Met Thr Cys Glu Ile Cys 130 135 140 Phe Glu Val His Pro Phe Glu Lys Met Arg Ala Pro Arg Cys Gly His 145 150 155 160 Tyr Phe Cys Glu Thr Cys Trp Thr Gly Tyr Ile His Thr Ala Ile Asn 165 170 175 Asp Gly Pro Gly Cys Leu Thr Leu Arg Cys Ala Asp Pro Ser Cys Gly 180 185 190 Ser Ala Ile Gly Glu Asp Met Val Leu Ser Leu Val Ser Thr Asp Asp 195 200 205 Gln Gln Lys Tyr Met Arg Tyr Leu Leu Arg Ser Tyr Val Glu Asp Asn 210 215 220 Arg Lys Val Lys Trp Cys Pro Ala Pro Gly Cys Glu Tyr Ala Val Glu 225 230 235 240 Phe Gln Pro Gly Val Gly Ser Tyr Asp Leu Val Cys Lys Cys Gly Phe 245 250 255 Asn Phe Cys Trp Asn Cys Arg Glu Glu Ala His Arg Pro Val Asp Cys 260 265 270 Glu Thr Val Asn Lys Trp Ile Leu Lys Asn Cys Ala Glu Ser Glu Asn 275 280 285 Met Asn Trp Ile Leu Ala Asn Ser Lys Pro Cys Pro Lys Cys Lys Arg 290 295 300 Pro Ile Glu Lys Asn Gln Gly Cys Met His Ile Thr Cys Thr Pro Pro 305 310 315 320 Cys Lys Phe Glu Phe Cys Trp Leu Cys Leu Gly Ala Trp Thr Asp His 325 330 335 Gly Glu Arg Thr Gly Gly Phe Tyr Ala Cys Asn Arg Tyr Glu Thr Ala 340 345 350 Lys Gln Glu Gly Val Tyr Asp Glu Ala Glu Arg Arg Arg Glu Met Ala 355 360 365 Lys Asn Ser Leu Glu Arg Tyr Thr His Tyr Tyr Glu Arg Trp Ala Thr 370 375 380 Asn Glu Ser Ser Arg Ala Lys Ala Leu Ala Asp Leu Gln Asp Met Gln 385 390 395 400 Asn Val Gln Ile Glu Lys Leu Ser Val Thr Gln Cys Gln Pro Val Ser 405 410 415 Gln Leu Lys Phe Val Thr Asp Ala Trp Leu Gln Ile Val Glu Cys Arg 420 425 430 Arg Val Leu Lys Trp Thr Tyr Ala Tyr Gly Tyr Tyr Leu Pro Glu Asn 435 440 445 Glu His Thr Lys Arg Gln Phe Phe Glu Tyr Ser Gln Gly Glu Ala Glu 450 455 460 Ala Gly Leu Glu Arg Leu His Gln Cys Ala Glu Lys Asp Leu Leu Thr 465 470 475 480 Phe Leu Gly Gly Thr Pro Thr Ser Ser Phe Asn Asp Phe Arg Thr Lys 485 490 495 Leu Ala Gly Leu Thr Ser Val Thr Lys Thr Tyr Phe Glu Asn Leu Val 500 505 510 Arg Ala Leu Glu Asn Asn Leu Ser Asp Val Asp Ile Pro Lys Ala Ala 515 520 525 Ala Lys Ser Ser Ser Ser Ser Lys Ala Ser Gly Ser Ser Lys Gly Arg 530 535 540 Gly Gly Arg Pro Lys Val Gly Ser Ser Lys Ser Gly Gly Ser Ser Arg 545 550 555 560 Ser Gly Glu Glu Ser Thr His Trp Ser Cys Glu His Cys Thr Tyr Ala 565 570 575 Asn Thr Thr Ala Ala Ser Ser Ile Val Cys Val Ile Cys Asn His Ala 580 585 590 Arg Ser 19884DNAPhyscomitrella patensCDS(248)..(520) 19atcccgggcc tgcgctcagc ctcgtcttcc tcgcattccg tcacctccgc ctgccctgca 60tcgaaacatc gatcccactc ctcgcatcct gctcttgcct tcgtcacctt gtgctctcga 120atctcgtacc ccgttttttg gttcgaaatt gcagttgaag atcgcgtgta tattggtatc 180tgcttgtggg agtgggagtg gaagtgtgct cacacccttg agccagaagt gagagaaact 240cggtaca atg ccc cag atc cag tac tcg gag aag tac ttc gac gat acc 289 Met Pro Gln Ile Gln Tyr Ser Glu Lys Tyr Phe Asp Asp Thr 1 5 10 tac gag tat cgg cat gtc gtg ctt ccc ccc gac att gcc aaa ttg ctg 337Tyr Glu Tyr Arg His Val Val Leu Pro Pro Asp Ile Ala Lys Leu Leu 15 20 25 30 ccc aaa aac cga ctc ctg tct gag gcc gaa tgg cgt ggt atc gga gtg 385Pro Lys Asn Arg Leu Leu Ser Glu Ala Glu Trp Arg Gly Ile Gly Val 35 40 45 cag cag tca cgt ggg tgg gtt cac tac gca att cac cgc cct gag cca 433Gln Gln Ser Arg Gly Trp Val His Tyr Ala Ile His Arg Pro Glu Pro 50 55 60 cac att atg ctc ttc cgt aga ccc ctg aat tac ggt caa cct cag caa 481His Ile Met Leu Phe Arg Arg Pro Leu Asn Tyr Gly Gln Pro Gln Gln 65 70 75 gct gcc gct gtg cag caa caa ccc aca ggc atg aaa gct taatcatcct 530Ala Ala Ala Val Gln Gln Gln Pro Thr Gly Met Lys Ala 80 85 90 atcaattgat tccaggaatt gaacatcatg tgctcatgat gttgtatggc cagtgctctc 590agttttttgg tcactatgtg atgttggata gttggtcctt aagtagtgac agctcttcgt 650actgtacaag atttgtcggg aagtagccat gttaattagt gaatgagcat cactgaggag 710tctctcgtga gctgattggt attctctgaa ggtggaagcg agcgggtgat agaaaactct 770tatgacttgc tttcctacaa atattgtgat acaattcttt tgatgcgctc ctcgtcaatc 830ttaagcacgt aggagacttc accatctgct actgcaacgc tcgtgcgagc tcgc 8842091PRTPhyscomitrella patens 20Met Pro Gln Ile Gln Tyr Ser Glu Lys Tyr Phe Asp Asp Thr Tyr Glu 1 5 10 15 Tyr Arg His Val Val Leu Pro Pro Asp Ile Ala Lys Leu Leu Pro Lys 20 25 30 Asn Arg Leu Leu Ser Glu Ala Glu Trp Arg Gly Ile Gly Val Gln Gln 35 40 45 Ser Arg Gly Trp Val His Tyr Ala Ile His Arg Pro Glu Pro His Ile 50 55 60 Met Leu Phe Arg Arg Pro Leu Asn Tyr Gly Gln Pro Gln Gln Ala Ala 65 70 75 80 Ala Val Gln Gln Gln Pro Thr Gly Met Lys Ala 85 90 211389DNAPhyscomitrella patensCDS(276)..(1316) 21atcccgggag tagcttggag tggcgttgca aggtgggatt aggatcgatt agttgttgat 60tgagtgcggc cctcggtgtt ggttatttgt ttaggtagat tgtagtctcg ctgggagtgg 120agaggaggta ttcgtttttt tggtttttat tgtttgacgt aggcttgtgg ttaggttttg 180acttatactt tctgttgagg aattgggaag aaaagaacag ggtgttctag catttagggg 240aattgaccag ggtaggaggt gaagccgaag cttcg atg tca tct gct gtg gac 293 Met Ser Ser Ala Val Asp 1 5 ttt gta gag ggc ggt acc caa gac ccg tgc gaa gat gcg tgc agc atc 341Phe Val Glu Gly Gly Thr Gln Asp Pro Cys Glu Asp Ala Cys Ser Ile 10 15 20 tgc ctt gaa act ttt tgt gaa gat gat cct gcc acc gtc act agc tgc 389Cys Leu Glu Thr Phe Cys Glu Asp Asp Pro Ala Thr Val Thr Ser Cys 25 30 35 aag cac gac tat cat ctg caa tgc att ctt gaa tgg tcg cag cgg agt 437Lys His Asp Tyr His Leu Gln Cys Ile Leu Glu Trp Ser Gln Arg Ser 40 45 50 acg gag tgt cca atg tgc ttg caa cca ctt agt ttg aaa gat cct gac 485Thr Glu Cys Pro Met Cys Leu Gln Pro Leu Ser Leu Lys Asp Pro Asp 55 60 65 70 agc caa gag ctg ctg aaa gca gtt ggg caa gaa cgg aca tta cgc cgg 533Ser Gln Glu Leu Leu Lys Ala Val Gly Gln Glu Arg Thr Leu Arg Arg 75 80 85 aat aag atg cag gcc tct cac att tac cgt cga tcc cca gct gag gag 581Asn Lys Met Gln Ala Ser His Ile Tyr Arg Arg Ser Pro Ala Glu Glu 90 95 100 tat gag ttc gaa cga ttt gca ccc tac ggg gac gaa ggt tgc att atg 629Tyr Glu Phe Glu Arg Phe Ala Pro Tyr Gly Asp Glu Gly Cys Ile Met 105 110 115 cag cat ttg gcg gcg gca gca atg ggc cgg aga gaa cat att cgc ttt 677Gln His Leu Ala Ala Ala Ala Met Gly Arg Arg Glu His Ile Arg Phe 120 125 130 cgg cca tcc act act gcc caa ggt cat ccg cat ttt gtt gtt gtg tct 725Arg Pro Ser Thr Thr Ala Gln Gly His Pro His Phe Val Val Val Ser 135 140 145 150 ggt gca cct gca gga gca tcg tct tcc cct gca tcc agt tcg cca gtt 773Gly Ala Pro Ala Gly Ala Ser Ser Ser Pro Ala Ser Ser Ser Pro Val 155 160 165 gta tcc cct cct caa agt aca aat ggc gag gca tct cta gga gcc gtc 821Val Ser Pro Pro Gln Ser Thr Asn Gly Glu Ala Ser Leu Gly Ala Val 170 175 180 ttc tca ttt cct cat tat tct gct ccc aat agg gac ggt agc tca gct 869Phe Ser Phe Pro His Tyr Ser Ala Pro Asn Arg Asp Gly Ser Ser Ala 185 190 195 aac tct cca acc att aga tca cgg agc ctt gag tcg gag gag cat gca 917Asn Ser Pro Thr Ile Arg Ser Arg Ser Leu Glu Ser Glu Glu His Ala 200 205 210 act tct tca tca gag tct cta gat acc ttt gca tct cgt ttg gtc gca 965Thr Ser Ser Ser Glu Ser Leu Asp Thr Phe Ala Ser Arg Leu Val Ala 215 220 225 230 gca tct tca agg tac aag gag tcc ctg agt aag agt acg aag gga ttc 1013Ala Ser Ser Arg Tyr Lys Glu Ser Leu Ser Lys Ser Thr Lys Gly Phe 235 240 245 cgt gaa agg ttg cga act cgt ggt ggt atc atg caa gac ctt ggt gca 1061Arg Glu Arg Leu Arg Thr Arg Gly Gly Ile Met Gln Asp Leu Gly Ala 250 255 260 cgg gca cga gag atg agt gca ggg atg gca cgg gcg tta gaa agg atg 1109Arg Ala Arg Glu Met Ser Ala Gly Met Ala Arg Ala Leu Glu Arg Met 265 270 275 tct gta gag aca gga act gat cgg tca gat gcg gcc agc tct ctt cct 1157Ser Val Glu Thr Gly Thr Asp Arg Ser Asp Ala Ala Ser Ser Leu Pro 280 285 290 ggg cag cct tct ggc act acc cat cac cca ccc gat cat cct cct gat 1205Gly Gln Pro Ser Gly Thr Thr His His Pro Pro Asp His Pro Pro Asp 295 300 305 310 cgc cct gat tct ggc cat tct tca gga ggg tcg ccg agt agc cac tct 1253Arg Pro Asp Ser Gly His Ser Ser Gly Gly Ser Pro Ser Ser His Ser 315 320 325 gcc gtc act cgc aca act cca agc ccc act tca gca cct gaa cta agt 1301Ala Val Thr Arg Thr Thr Pro Ser Pro Thr Ser Ala Pro Glu Leu Ser 330 335 340 aca ggg aca gaa cac taggtttaaa actacttagt tcaaacatat cactttcgaa 1356Thr Gly Thr Glu His 345 taagagcgga tgctggcatc cgtccgagct cgc 1389 22347PRTPhyscomitrella patens 22Met Ser Ser Ala Val Asp Phe Val Glu Gly Gly Thr Gln Asp Pro Cys 1 5 10 15 Glu Asp Ala Cys Ser Ile Cys Leu Glu Thr Phe Cys Glu Asp Asp Pro 20 25 30 Ala Thr Val Thr Ser Cys Lys His Asp Tyr His Leu Gln Cys Ile Leu 35 40 45 Glu Trp Ser Gln Arg Ser Thr Glu Cys Pro Met Cys Leu Gln Pro Leu 50 55 60 Ser Leu Lys Asp Pro Asp Ser Gln Glu Leu Leu Lys Ala Val Gly Gln 65 70 75 80 Glu Arg Thr Leu Arg Arg Asn Lys Met Gln Ala Ser His Ile Tyr Arg 85 90 95 Arg Ser Pro Ala Glu Glu Tyr Glu Phe Glu Arg Phe Ala Pro Tyr Gly 100 105 110 Asp Glu Gly Cys Ile Met Gln His Leu Ala Ala Ala Ala Met Gly Arg 115 120 125 Arg Glu His Ile Arg Phe Arg Pro Ser Thr Thr Ala Gln Gly His Pro 130 135 140 His Phe Val Val Val Ser Gly Ala Pro Ala Gly Ala Ser Ser Ser Pro 145 150 155 160 Ala Ser Ser Ser Pro Val Val Ser Pro Pro Gln Ser Thr Asn Gly Glu 165 170 175 Ala Ser Leu Gly Ala Val Phe Ser Phe Pro His Tyr Ser Ala Pro Asn 180 185 190 Arg Asp Gly Ser Ser Ala Asn Ser Pro Thr Ile Arg Ser Arg Ser Leu 195 200 205 Glu Ser Glu Glu His Ala Thr Ser Ser Ser Glu Ser Leu Asp Thr Phe 210 215 220 Ala Ser Arg Leu Val Ala Ala Ser Ser Arg Tyr Lys Glu Ser Leu Ser 225 230 235 240 Lys Ser Thr Lys Gly Phe Arg Glu Arg Leu Arg Thr Arg Gly Gly Ile 245 250 255 Met Gln Asp Leu Gly Ala Arg Ala Arg Glu Met Ser Ala Gly Met Ala 260 265 270 Arg Ala Leu Glu Arg Met Ser Val Glu Thr Gly Thr Asp Arg Ser Asp 275 280 285 Ala Ala Ser Ser Leu Pro Gly Gln Pro Ser Gly Thr Thr His His Pro 290 295 300 Pro Asp His Pro Pro Asp Arg Pro Asp Ser Gly His Ser Ser Gly Gly 305 310 315 320 Ser Pro Ser Ser His Ser Ala Val Thr Arg Thr Thr Pro Ser Pro Thr 325 330 335 Ser Ala Pro Glu Leu Ser Thr Gly Thr Glu His 340 345 232776DNAPhyscomitrella patensCDS(127)..(2568) 23cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attgggccct 60ctagatgcat gctcgagcgg ccgccagtgt gatggatatc tgcagaattc gcccttatcc 120cgggcc atg gct gtg tcc cgt ctt ggt ccc gct cca agt cca tcg gcc 168 Met Ala Val Ser Arg Leu Gly Pro Ala Pro Ser Pro Ser Ala 1 5 10 gtt caa tgg agt ctc tcc agc agg cct ctc cct ctc gca acg agt agg 216Val Gln Trp Ser Leu Ser Ser Arg Pro Leu Pro Leu Ala Thr Ser Arg 15 20 25 30 ttc agt gtg gtt cca gtt cgc gct tcg aaa aat gta gaa gat gga gat 264Phe Ser Val Val Pro Val Arg Ala Ser Lys Asn Val Glu Asp Gly Asp 35 40 45 act agt ggt ggg tgc gcg gct cta tca cgt cga gct ttg att gct ttg

312Thr Ser Gly Gly Cys Ala Ala Leu Ser Arg Arg Ala Leu Ile Ala Leu 50 55 60 ata gct ctg tct act caa ttg ggt ggt gtt gcg tcg gcc cga gac att 360Ile Ala Leu Ser Thr Gln Leu Gly Gly Val Ala Ser Ala Arg Asp Ile 65 70 75 agt ggt ctc ata gag tct tca gtg ggc gag gag gtg tcc tca tta tcc 408Ser Gly Leu Ile Glu Ser Ser Val Gly Glu Glu Val Ser Ser Leu Ser 80 85 90 ctg agt gca gtt gaa gta ccg aac acg cct aaa atc tct cca ata agc 456Leu Ser Ala Val Glu Val Pro Asn Thr Pro Lys Ile Ser Pro Ile Ser 95 100 105 110 act gac cta ggg att ata aat gaa gtg cca aga gct ctt gcg gac agt 504Thr Asp Leu Gly Ile Ile Asn Glu Val Pro Arg Ala Leu Ala Asp Ser 115 120 125 gga gtt ggt gca gtt gag gaa aag ata aac cag tca gca tct gag gtt 552Gly Val Gly Ala Val Glu Glu Lys Ile Asn Gln Ser Ala Ser Glu Val 130 135 140 tca tct ggt ggg agt aat ggg ttt gga cct tta agt ctt ggg ggt atc 600Ser Ser Gly Gly Ser Asn Gly Phe Gly Pro Leu Ser Leu Gly Gly Ile 145 150 155 tta gga acc ggt gtg gcg ggg gcg ttg ttt tat agc gag cga caa tct 648Leu Gly Thr Gly Val Ala Gly Ala Leu Phe Tyr Ser Glu Arg Gln Ser 160 165 170 aaa gct cag gct gaa tct gca ctt gat gct gcg aaa aag cag ctc cag 696Lys Ala Gln Ala Glu Ser Ala Leu Asp Ala Ala Lys Lys Gln Leu Gln 175 180 185 190 gag ctt aga gag acc tca gaa ggg caa tta ctt gca gaa aag cag cta 744Glu Leu Arg Glu Thr Ser Glu Gly Gln Leu Leu Ala Glu Lys Gln Leu 195 200 205 gca caa aag gag gcg agc aag gcc cag gag caa cgc aca acg ctt act 792Ala Gln Lys Glu Ala Ser Lys Ala Gln Glu Gln Arg Thr Thr Leu Thr 210 215 220 aat gag ctc atg gct tca aga tcc tcg gtt act gat ctg gaa ggt aag 840Asn Glu Leu Met Ala Ser Arg Ser Ser Val Thr Asp Leu Glu Gly Lys 225 230 235 ctt caa atg gca aaa gct tca gtg gtc gat ctt cag gaa agg gtt tca 888Leu Gln Met Ala Lys Ala Ser Val Val Asp Leu Gln Glu Arg Val Ser 240 245 250 agt ctg caa gtc act ctc gcg gac caa gag aag aat tac agt tca ctg 936Ser Leu Gln Val Thr Leu Ala Asp Gln Glu Lys Asn Tyr Ser Ser Leu 255 260 265 270 aat gga aga ttt gta gag gaa aaa gaa gta agt gaa aag cta cgg aat 984Asn Gly Arg Phe Val Glu Glu Lys Glu Val Ser Glu Lys Leu Arg Asn 275 280 285 gaa att aca acg tta aaa tat acg ctt tcg gac aaa gaa aag gat tac 1032Glu Ile Thr Thr Leu Lys Tyr Thr Leu Ser Asp Lys Glu Lys Asp Tyr 290 295 300 aca tca ctc aat gag aga ttt gtg gag gag aaa gca act gct gaa gag 1080Thr Ser Leu Asn Glu Arg Phe Val Glu Glu Lys Ala Thr Ala Glu Glu 305 310 315 ctg cag gag aag att aaa gta ttg aag atg gac atc gag gct aag gaa 1128Leu Gln Glu Lys Ile Lys Val Leu Lys Met Asp Ile Glu Ala Lys Glu 320 325 330 aat gaa att aat gtt caa act gca agg atc aaa gaa gaa cag gac tct 1176Asn Glu Ile Asn Val Gln Thr Ala Arg Ile Lys Glu Glu Gln Asp Ser 335 340 345 350 gtt gca tcg ttg caa aat gag ctt caa att gca gga aca caa ctt tct 1224Val Ala Ser Leu Gln Asn Glu Leu Gln Ile Ala Gly Thr Gln Leu Ser 355 360 365 gaa gag cgc agt aga ctg act gaa gtg agc agt aaa ctt gca act ctt 1272Glu Glu Arg Ser Arg Leu Thr Glu Val Ser Ser Lys Leu Ala Thr Leu 370 375 380 gag ggt agt tat gct gct tca cag gat tta aat aca cag ctg gat ctt 1320Glu Gly Ser Tyr Ala Ala Ser Gln Asp Leu Asn Thr Gln Leu Asp Leu 385 390 395 tcg atc agt gat ttg aaa caa aaa cta cag act aca aac agt aga aag 1368Ser Ile Ser Asp Leu Lys Gln Lys Leu Gln Thr Thr Asn Ser Arg Lys 400 405 410 gag gcg ctc gag aaa gag gtg aca agc ttg aat gaa gtg ata aac tcg 1416Glu Ala Leu Glu Lys Glu Val Thr Ser Leu Asn Glu Val Ile Asn Ser 415 420 425 430 ctg aaa gga acc ttg gca gaa gag aat gac aag aag gac acc ctg tat 1464Leu Lys Gly Thr Leu Ala Glu Glu Asn Asp Lys Lys Asp Thr Leu Tyr 435 440 445 ggc cag ctc aag gta act tca ggg gct tta gag aag gca aca tcg gag 1512Gly Gln Leu Lys Val Thr Ser Gly Ala Leu Glu Lys Ala Thr Ser Glu 450 455 460 gtg cag ttg ctg gag caa cgg gtt acg aat atg tct gca gct gta aaa 1560Val Gln Leu Leu Glu Gln Arg Val Thr Asn Met Ser Ala Ala Val Lys 465 470 475 gca ctt gaa aaa gag aaa aat ggt gag atc aat caa ctt acc aag gaa 1608Ala Leu Glu Lys Glu Lys Asn Gly Glu Ile Asn Gln Leu Thr Lys Glu 480 485 490 ctc caa gag aga att aag tca ctt gat gtg gcc cag caa aaa tgt caa 1656Leu Gln Glu Arg Ile Lys Ser Leu Asp Val Ala Gln Gln Lys Cys Gln 495 500 505 510 gca ttt tct aac gaa ata tcc act ctc aag agg cag cag gca gct cta 1704Ala Phe Ser Asn Glu Ile Ser Thr Leu Lys Arg Gln Gln Ala Ala Leu 515 520 525 aat gaa gag ttg gac aac aca aac aaa gaa ttg gag gca tca acc gat 1752Asn Glu Glu Leu Asp Asn Thr Asn Lys Glu Leu Glu Ala Ser Thr Asp 530 535 540 gaa ttt aaa acc ata agt gag cag cta aca gtc gct gtt aac tta agt 1800Glu Phe Lys Thr Ile Ser Glu Gln Leu Thr Val Ala Val Asn Leu Ser 545 550 555 ttg aag ctg gaa acc caa tta aac gag acg aga gca gca tat caa agc 1848Leu Lys Leu Glu Thr Gln Leu Asn Glu Thr Arg Ala Ala Tyr Gln Ser 560 565 570 gca aat gtt gca ctt gca gag gag cga aag gta aca gct gct gct aag 1896Ala Asn Val Ala Leu Ala Glu Glu Arg Lys Val Thr Ala Ala Ala Lys 575 580 585 590 aat cag tta gct aca aca caa agg tct ctc ata gcg gaa aag aac agt 1944Asn Gln Leu Ala Thr Thr Gln Arg Ser Leu Ile Ala Glu Lys Asn Ser 595 600 605 gta aaa gct ctg cga gga agt gtg gac caa gct ctc cag gca ctt cag 1992Val Lys Ala Leu Arg Gly Ser Val Asp Gln Ala Leu Gln Ala Leu Gln 610 615 620 gag ctc aac caa gat tca gtc gcc tta gca gat gag ctc gac aaa gca 2040Glu Leu Asn Gln Asp Ser Val Ala Leu Ala Asp Glu Leu Asp Lys Ala 625 630 635 aaa aag aaa atc gcc aac ttg gag gca gag agt gca tca gta cgt caa 2088Lys Lys Lys Ile Ala Asn Leu Glu Ala Glu Ser Ala Ser Val Arg Gln 640 645 650 aag ctt ggt aag gag aaa gaa atg tct gct aat tta aga tca ggg gct 2136Lys Leu Gly Lys Glu Lys Glu Met Ser Ala Asn Leu Arg Ser Gly Ala 655 660 665 670 gca gaa gct gaa ggt act ata gct agg ctc ctt aag gag aat gac gcc 2184Ala Glu Ala Glu Gly Thr Ile Ala Arg Leu Leu Lys Glu Asn Asp Ala 675 680 685 ggc aat aaa aag gtg aag cag ttg gaa ggt gag gta ctg aag agc aaa 2232Gly Asn Lys Lys Val Lys Gln Leu Glu Gly Glu Val Leu Lys Ser Lys 690 695 700 ggg gag acg gcc aaa cag aag gga aag ctt ttg gaa caa aaa cgt gca 2280Gly Glu Thr Ala Lys Gln Lys Gly Lys Leu Leu Glu Gln Lys Arg Ala 705 710 715 ttg caa caa gct gag aca cgt ctg aaa atg atc cct cag gtt cgt gcg 2328Leu Gln Gln Ala Glu Thr Arg Leu Lys Met Ile Pro Gln Val Arg Ala 720 725 730 gag gct gca tta ttg gtt gag aag tac gag gat cta gct tat caa gaa 2376Glu Ala Ala Leu Leu Val Glu Lys Tyr Glu Asp Leu Ala Tyr Gln Glu 735 740 745 750 aaa gag caa aag gaa gct ata atg cgt gag aat gaa cag ctg aat aag 2424Lys Glu Gln Lys Glu Ala Ile Met Arg Glu Asn Glu Gln Leu Asn Lys 755 760 765 tct ggc aag acg gtc ggt gca gac ctc agt gag gtt aaa cag agc tta 2472Ser Gly Lys Thr Val Gly Ala Asp Leu Ser Glu Val Lys Gln Ser Leu 770 775 780 gag gac ggt caa ggc agc acg atc gat atc gaa ggg cga att cca gca 2520Glu Asp Gly Gln Gly Ser Thr Ile Asp Ile Glu Gly Arg Ile Pro Ala 785 790 795 cac tgg cgg ccg tta cta gtg gat ccg agc tcg gta cca agc ttg gcg 2568His Trp Arg Pro Leu Leu Val Asp Pro Ser Ser Val Pro Ser Leu Ala 800 805 810 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 2628atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 2688ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 2748taatgaatcg gccaacgcgc ggggagaa 277624814PRTPhyscomitrella patens 24Met Ala Val Ser Arg Leu Gly Pro Ala Pro Ser Pro Ser Ala Val Gln 1 5 10 15 Trp Ser Leu Ser Ser Arg Pro Leu Pro Leu Ala Thr Ser Arg Phe Ser 20 25 30 Val Val Pro Val Arg Ala Ser Lys Asn Val Glu Asp Gly Asp Thr Ser 35 40 45 Gly Gly Cys Ala Ala Leu Ser Arg Arg Ala Leu Ile Ala Leu Ile Ala 50 55 60 Leu Ser Thr Gln Leu Gly Gly Val Ala Ser Ala Arg Asp Ile Ser Gly 65 70 75 80 Leu Ile Glu Ser Ser Val Gly Glu Glu Val Ser Ser Leu Ser Leu Ser 85 90 95 Ala Val Glu Val Pro Asn Thr Pro Lys Ile Ser Pro Ile Ser Thr Asp 100 105 110 Leu Gly Ile Ile Asn Glu Val Pro Arg Ala Leu Ala Asp Ser Gly Val 115 120 125 Gly Ala Val Glu Glu Lys Ile Asn Gln Ser Ala Ser Glu Val Ser Ser 130 135 140 Gly Gly Ser Asn Gly Phe Gly Pro Leu Ser Leu Gly Gly Ile Leu Gly 145 150 155 160 Thr Gly Val Ala Gly Ala Leu Phe Tyr Ser Glu Arg Gln Ser Lys Ala 165 170 175 Gln Ala Glu Ser Ala Leu Asp Ala Ala Lys Lys Gln Leu Gln Glu Leu 180 185 190 Arg Glu Thr Ser Glu Gly Gln Leu Leu Ala Glu Lys Gln Leu Ala Gln 195 200 205 Lys Glu Ala Ser Lys Ala Gln Glu Gln Arg Thr Thr Leu Thr Asn Glu 210 215 220 Leu Met Ala Ser Arg Ser Ser Val Thr Asp Leu Glu Gly Lys Leu Gln 225 230 235 240 Met Ala Lys Ala Ser Val Val Asp Leu Gln Glu Arg Val Ser Ser Leu 245 250 255 Gln Val Thr Leu Ala Asp Gln Glu Lys Asn Tyr Ser Ser Leu Asn Gly 260 265 270 Arg Phe Val Glu Glu Lys Glu Val Ser Glu Lys Leu Arg Asn Glu Ile 275 280 285 Thr Thr Leu Lys Tyr Thr Leu Ser Asp Lys Glu Lys Asp Tyr Thr Ser 290 295 300 Leu Asn Glu Arg Phe Val Glu Glu Lys Ala Thr Ala Glu Glu Leu Gln 305 310 315 320 Glu Lys Ile Lys Val Leu Lys Met Asp Ile Glu Ala Lys Glu Asn Glu 325 330 335 Ile Asn Val Gln Thr Ala Arg Ile Lys Glu Glu Gln Asp Ser Val Ala 340 345 350 Ser Leu Gln Asn Glu Leu Gln Ile Ala Gly Thr Gln Leu Ser Glu Glu 355 360 365 Arg Ser Arg Leu Thr Glu Val Ser Ser Lys Leu Ala Thr Leu Glu Gly 370 375 380 Ser Tyr Ala Ala Ser Gln Asp Leu Asn Thr Gln Leu Asp Leu Ser Ile 385 390 395 400 Ser Asp Leu Lys Gln Lys Leu Gln Thr Thr Asn Ser Arg Lys Glu Ala 405 410 415 Leu Glu Lys Glu Val Thr Ser Leu Asn Glu Val Ile Asn Ser Leu Lys 420 425 430 Gly Thr Leu Ala Glu Glu Asn Asp Lys Lys Asp Thr Leu Tyr Gly Gln 435 440 445 Leu Lys Val Thr Ser Gly Ala Leu Glu Lys Ala Thr Ser Glu Val Gln 450 455 460 Leu Leu Glu Gln Arg Val Thr Asn Met Ser Ala Ala Val Lys Ala Leu 465 470 475 480 Glu Lys Glu Lys Asn Gly Glu Ile Asn Gln Leu Thr Lys Glu Leu Gln 485 490 495 Glu Arg Ile Lys Ser Leu Asp Val Ala Gln Gln Lys Cys Gln Ala Phe 500 505 510 Ser Asn Glu Ile Ser Thr Leu Lys Arg Gln Gln Ala Ala Leu Asn Glu 515 520 525 Glu Leu Asp Asn Thr Asn Lys Glu Leu Glu Ala Ser Thr Asp Glu Phe 530 535 540 Lys Thr Ile Ser Glu Gln Leu Thr Val Ala Val Asn Leu Ser Leu Lys 545 550 555 560 Leu Glu Thr Gln Leu Asn Glu Thr Arg Ala Ala Tyr Gln Ser Ala Asn 565 570 575 Val Ala Leu Ala Glu Glu Arg Lys Val Thr Ala Ala Ala Lys Asn Gln 580 585 590 Leu Ala Thr Thr Gln Arg Ser Leu Ile Ala Glu Lys Asn Ser Val Lys 595 600 605 Ala Leu Arg Gly Ser Val Asp Gln Ala Leu Gln Ala Leu Gln Glu Leu 610 615 620 Asn Gln Asp Ser Val Ala Leu Ala Asp Glu Leu Asp Lys Ala Lys Lys 625 630 635 640 Lys Ile Ala Asn Leu Glu Ala Glu Ser Ala Ser Val Arg Gln Lys Leu 645 650 655 Gly Lys Glu Lys Glu Met Ser Ala Asn Leu Arg Ser Gly Ala Ala Glu 660 665 670 Ala Glu Gly Thr Ile Ala Arg Leu Leu Lys Glu Asn Asp Ala Gly Asn 675 680 685 Lys Lys Val Lys Gln Leu Glu Gly Glu Val Leu Lys Ser Lys Gly Glu 690 695 700 Thr Ala Lys Gln Lys Gly Lys Leu Leu Glu Gln Lys Arg Ala Leu Gln 705 710 715 720 Gln Ala Glu Thr Arg Leu Lys Met Ile Pro Gln Val Arg Ala Glu Ala 725 730 735 Ala Leu Leu Val Glu Lys Tyr Glu Asp Leu Ala Tyr Gln Glu Lys Glu 740 745 750 Gln Lys Glu Ala Ile Met Arg Glu Asn Glu Gln Leu Asn Lys Ser Gly 755 760 765 Lys Thr Val Gly Ala Asp Leu Ser Glu Val Lys Gln Ser Leu Glu Asp 770 775 780 Gly Gln Gly Ser Thr Ile Asp Ile Glu Gly Arg Ile Pro Ala His Trp 785 790 795 800 Arg Pro Leu Leu Val Asp Pro Ser Ser Val Pro Ser Leu Ala 805 810 251461DNAPhyscomitrella patensCDS(225)..(977) 25atcccgggag agcctccact acgtctgtcg aatgctcaca gcgcatgacg ttgaatcccc 60aagccgacag tttcttgaga agagaaaaat acagtcggag tgttctgttc tccatcacca 120ttatcgctga tgaagaagtg aggagcttct gagaggcaga gttgcatcag gttgtaacaa 180aacgcgattc cttcagtgtc gaatcaccag caggaggctt aaaa atg gta ggg aat 236 Met Val Gly Asn 1 ggc aga aga atg aac cgc ttg gtg gcg ttc tta gtg gtg gtc tgt tca 284Gly Arg Arg Met Asn Arg Leu Val Ala Phe Leu Val Val Val Cys Ser 5 10 15 20 gcc gtc tca gga tgc aga gca tgg gac tgc tcg gca gcg gat aaa cag 332Ala Val Ser Gly Cys Arg Ala Trp Asp Cys Ser Ala Ala Asp Lys Gln 25 30 35 aca ctg cta gac ttc aag aat ggg ttc gtg gac acg aac gga gtg ttc 380Thr Leu Leu Asp Phe Lys Asn Gly Phe Val Asp Thr Asn Gly Val Phe 40

45 50 aac acc tgg agt gat agc act gtg aac tgc tgc gca tgg aag ggc atc 428Asn Thr Trp Ser Asp Ser Thr Val Asn Cys Cys Ala Trp Lys Gly Ile 55 60 65 aca tgt cgc gag tca gat ggc gca att ttg gag atc aac atc gtg gga 476Thr Cys Arg Glu Ser Asp Gly Ala Ile Leu Glu Ile Asn Ile Val Gly 70 75 80 tcc tct ggc aca aac cag cag cca tac cgc agc ccg agc tac caa ggc 524Ser Ser Gly Thr Asn Gln Gln Pro Tyr Arg Ser Pro Ser Tyr Gln Gly 85 90 95 100 aca gtt ggc gca ggg ctg gtg gcg ctc acc caa ttg cag aaa ctc aag 572Thr Val Gly Ala Gly Leu Val Ala Leu Thr Gln Leu Gln Lys Leu Lys 105 110 115 atc gag tgg gtg ctc ttc aac ggc ccc atc cct cag cag tgg gga gat 620Ile Glu Trp Val Leu Phe Asn Gly Pro Ile Pro Gln Gln Trp Gly Asp 120 125 130 ttc tcc acc act ctc gtg ttg atc acc atc aac aac gcc aac ctc cgc 668Phe Ser Thr Thr Leu Val Leu Ile Thr Ile Asn Asn Ala Asn Leu Arg 135 140 145 aac gac ata ccc tcc acc ctg gtt aac atc cag aac cta cgg cac ctg 716Asn Asp Ile Pro Ser Thr Leu Val Asn Ile Gln Asn Leu Arg His Leu 150 155 160 gac ctc aag aac aac cac ctt acg ggc tcc atc ccc tcc acc ttc tgc 764Asp Leu Lys Asn Asn His Leu Thr Gly Ser Ile Pro Ser Thr Phe Cys 165 170 175 180 acg cac aag aag atc aac tac atc gat gtc tcc tac aac gac atg acc 812Thr His Lys Lys Ile Asn Tyr Ile Asp Val Ser Tyr Asn Asp Met Thr 185 190 195 tac ctt ctt gtc cct ccg tgc tta gta aac caa aat aac ctc acc gtc 860Tyr Leu Leu Val Pro Pro Cys Leu Val Asn Gln Asn Asn Leu Thr Val 200 205 210 atc ttt gat cac cag ggt aac agt act agc ccc ggc tat cct gcg gca 908Ile Phe Asp His Gln Gly Asn Ser Thr Ser Pro Gly Tyr Pro Ala Ala 215 220 225 ggc tcc acc ctc aca gtc tcg tcg ttg ctc ctc gca atc gga gct ctc 956Gly Ser Thr Leu Thr Val Ser Ser Leu Leu Leu Ala Ile Gly Ala Leu 230 235 240 acc act gct ctc ctc ttc ctc tgaggaaatc cacccccttt cctcgtggtc 1007Thr Thr Ala Leu Leu Phe Leu 245 250 aggcaaaccc taactcatta ctgcaatacc cagaacacag ttcttgagaa gggaattttg 1067atgccgtctc catccctaac tctctcgctc tcactcgtgc gcgcgcacgc gggatctctt 1127cgttatgaaa aaattaataa gtgccccgga gagattttgt ctgtgatgtc ggaatttgca 1187gaggaatgaa gcagtcaggt gcacgtaatg tccaaaaggt aacaatcgaa tcacaaggat 1247attgtagagt tgtcaatgga accgtggcta cacgccagga aaaaagtgag tacatattgt 1307ttgatccttg ggatgtatgc tgtattagtg aaaacctttg tcaaggacag cgctactctg 1367ttgaatgttt aagcttaagc tgggctgtaa catccagtta aggaataaag ccttaactat 1427tagttgtctc ggctgtgcga ttatccgata tccg 146126251PRTPhyscomitrella patens 26Met Val Gly Asn Gly Arg Arg Met Asn Arg Leu Val Ala Phe Leu Val 1 5 10 15 Val Val Cys Ser Ala Val Ser Gly Cys Arg Ala Trp Asp Cys Ser Ala 20 25 30 Ala Asp Lys Gln Thr Leu Leu Asp Phe Lys Asn Gly Phe Val Asp Thr 35 40 45 Asn Gly Val Phe Asn Thr Trp Ser Asp Ser Thr Val Asn Cys Cys Ala 50 55 60 Trp Lys Gly Ile Thr Cys Arg Glu Ser Asp Gly Ala Ile Leu Glu Ile 65 70 75 80 Asn Ile Val Gly Ser Ser Gly Thr Asn Gln Gln Pro Tyr Arg Ser Pro 85 90 95 Ser Tyr Gln Gly Thr Val Gly Ala Gly Leu Val Ala Leu Thr Gln Leu 100 105 110 Gln Lys Leu Lys Ile Glu Trp Val Leu Phe Asn Gly Pro Ile Pro Gln 115 120 125 Gln Trp Gly Asp Phe Ser Thr Thr Leu Val Leu Ile Thr Ile Asn Asn 130 135 140 Ala Asn Leu Arg Asn Asp Ile Pro Ser Thr Leu Val Asn Ile Gln Asn 145 150 155 160 Leu Arg His Leu Asp Leu Lys Asn Asn His Leu Thr Gly Ser Ile Pro 165 170 175 Ser Thr Phe Cys Thr His Lys Lys Ile Asn Tyr Ile Asp Val Ser Tyr 180 185 190 Asn Asp Met Thr Tyr Leu Leu Val Pro Pro Cys Leu Val Asn Gln Asn 195 200 205 Asn Leu Thr Val Ile Phe Asp His Gln Gly Asn Ser Thr Ser Pro Gly 210 215 220 Tyr Pro Ala Ala Gly Ser Thr Leu Thr Val Ser Ser Leu Leu Leu Ala 225 230 235 240 Ile Gly Ala Leu Thr Thr Ala Leu Leu Phe Leu 245 250 272489DNAPhyscomitrella patensCDS(145)..(2211) 27atcccgggag gaaccatgtg tgtagagagg ccttgtcaat tctctgtggg catgaggcct 60tgtagacatg gctccattgt gaggtagcaa ttatgtttgt agacagacct tgtctatcct 120ctgtggggtt gtgtccttgt agac atg gca ggc att tcg agg gat atc cgc 171 Met Ala Gly Ile Ser Arg Asp Ile Arg 1 5 gga aca gta gag aat ttg gtc cgt ctg acc tcc gat agc aac agc acc 219Gly Thr Val Glu Asn Leu Val Arg Leu Thr Ser Asp Ser Asn Ser Thr 10 15 20 25 gat cca ctc aaa ctc aac gtt cta caa tgt cag ttg gtt gct aag aaa 267Asp Pro Leu Lys Leu Asn Val Leu Gln Cys Gln Leu Val Ala Lys Lys 30 35 40 gcg gcc gac agt tcg tac acc ttg aac gac ctt gag gct tat cag cgc 315Ala Ala Asp Ser Ser Tyr Thr Leu Asn Asp Leu Glu Ala Tyr Gln Arg 45 50 55 agg cat gag aaa agc tcc gat gga cgc atc gct ggt acc gga ttt cga 363Arg His Glu Lys Ser Ser Asp Gly Arg Ile Ala Gly Thr Gly Phe Arg 60 65 70 gcc gct aag gaa ctt ctc cga gtg ctg aag gac gcc gag att ctt atc 411Ala Ala Lys Glu Leu Leu Arg Val Leu Lys Asp Ala Glu Ile Leu Ile 75 80 85 aga gag tgc tgc tct gaa aag tgg aag aaa gtg gtg ttc aaa cgc gga 459Arg Glu Cys Cys Ser Glu Lys Trp Lys Lys Val Val Phe Lys Arg Gly 90 95 100 105 aaa ttg caa gag act ttt gcg aag ata gcg tat gag att gag tgg cac 507Lys Leu Gln Glu Thr Phe Ala Lys Ile Ala Tyr Glu Ile Glu Trp His 110 115 120 tcg ttg gtg ttg tac agc gtc ttg gtg gca cag agc gac atc tat gac 555Ser Leu Val Leu Tyr Ser Val Leu Val Ala Gln Ser Asp Ile Tyr Asp 125 130 135 aag agg acg tgc gat ggt aag ctg agg tcc atc gac cat gcc agg ttg 603Lys Arg Thr Cys Asp Gly Lys Leu Arg Ser Ile Asp His Ala Arg Leu 140 145 150 ata ctt gcg gca agg caa gac cta gat tct ttg agg gcc ctt ctc caa 651Ile Leu Ala Ala Arg Gln Asp Leu Asp Ser Leu Arg Ala Leu Leu Gln 155 160 165 ggc cca cac gtt tgt gat gaa ata tgt aaa cca gac ttt tgt tcc gaa 699Gly Pro His Val Cys Asp Glu Ile Cys Lys Pro Asp Phe Cys Ser Glu 170 175 180 185 tgc ctc aag gat aaa gta ctt cag cag tgg gat aca gaa gaa aaa gaa 747Cys Leu Lys Asp Lys Val Leu Gln Gln Trp Asp Thr Glu Glu Lys Glu 190 195 200 ttg gag gac agg agc tct ctg tcg caa att atg tca tct atc ttc tca 795Leu Glu Asp Arg Ser Ser Leu Ser Gln Ile Met Ser Ser Ile Phe Ser 205 210 215 tgg gtt caa ccg cag ttt gat tct aaa aga caa cag aat gga aaa gta 843Trp Val Gln Pro Gln Phe Asp Ser Lys Arg Gln Gln Asn Gly Lys Val 220 225 230 ggc gtt gct gaa gtg aaa gag ata aaa tgg ctt ggg caa atg tat gca 891Gly Val Ala Glu Val Lys Glu Ile Lys Trp Leu Gly Gln Met Tyr Ala 235 240 245 atg aag act ttc aac aaa gat gcg act cac gag gca cac ttc agg gag 939Met Lys Thr Phe Asn Lys Asp Ala Thr His Glu Ala His Phe Arg Glu 250 255 260 265 gag gtt tct gat atg gct gcc ctt gac cat ccg aat gta gtc cgt atc 987Glu Val Ser Asp Met Ala Ala Leu Asp His Pro Asn Val Val Arg Ile 270 275 280 atc tgt tgt tgg gaa gac aaa aac tac gta agt atc ttg atg gaa ccg 1035Ile Cys Cys Trp Glu Asp Lys Asn Tyr Val Ser Ile Leu Met Glu Pro 285 290 295 ctg cgc aaa agc ttg cac aat ctg cta ctg aat tac aaa gat gga act 1083Leu Arg Lys Ser Leu His Asn Leu Leu Leu Asn Tyr Lys Asp Gly Thr 300 305 310 cat gct ccg tct gca cct acc cca ttt aca atc tta aat tca gtc gat 1131His Ala Pro Ser Ala Pro Thr Pro Phe Thr Ile Leu Asn Ser Val Asp 315 320 325 att atg ctg caa att gcc gaa ggc gtc aga tat gtc cac agc aaa aac 1179Ile Met Leu Gln Ile Ala Glu Gly Val Arg Tyr Val His Ser Lys Asn 330 335 340 345 ttt act cac ctt gac atc atg tcg ctc aat gtt cta gta caa ttt gcc 1227Phe Thr His Leu Asp Ile Met Ser Leu Asn Val Leu Val Gln Phe Ala 350 355 360 gat cct atc acc tca aca gat gtg aag gat tct gat aca gtg acc att 1275Asp Pro Ile Thr Ser Thr Asp Val Lys Asp Ser Asp Thr Val Thr Ile 365 370 375 tcc agc aga tct aca tct ttc acg gtc aaa ctt gca gac ttc ggt ttg 1323Ser Ser Arg Ser Thr Ser Phe Thr Val Lys Leu Ala Asp Phe Gly Leu 380 385 390 aag agg ata atc aat gaa aaa ggt cgt cgg aca tca aac tct gtc aag 1371Lys Arg Ile Ile Asn Glu Lys Gly Arg Arg Thr Ser Asn Ser Val Lys 395 400 405 aca gca tgg aca gct cca gag gct tac aag ctc aga aaa ggc gaa gat 1419Thr Ala Trp Thr Ala Pro Glu Ala Tyr Lys Leu Arg Lys Gly Glu Asp 410 415 420 425 tca gcc tgg ttc cac ccc agg aaa gca gac gtt tat agc ttt gcg ata 1467Ser Ala Trp Phe His Pro Arg Lys Ala Asp Val Tyr Ser Phe Ala Ile 430 435 440 acg tgc tcc gag ata ctt aca gga gat cac cct ttc gca cat ttc aat 1515Thr Cys Ser Glu Ile Leu Thr Gly Asp His Pro Phe Ala His Phe Asn 445 450 455 gcg gac tac aat ttc gat gct gta aag gat ggg gac cgg ccg agg ttg 1563Ala Asp Tyr Asn Phe Asp Ala Val Lys Asp Gly Asp Arg Pro Arg Leu 460 465 470 cca ggg gaa act ccc agg cga ttg gct gct ttg att cat cga tgc tgg 1611Pro Gly Glu Thr Pro Arg Arg Leu Ala Ala Leu Ile His Arg Cys Trp 475 480 485 cac cga aac cct caa cta cgc cct gat ttt acc gca att tgc acg gag 1659His Arg Asn Pro Gln Leu Arg Pro Asp Phe Thr Ala Ile Cys Thr Glu 490 495 500 505 ctt cga ttc atc aag ggg ctt gcc ttg cga ggt gat atc aaa tca ctg 1707Leu Arg Phe Ile Lys Gly Leu Ala Leu Arg Gly Asp Ile Lys Ser Leu 510 515 520 cac caa aca gat gtt ggc aat gaa gcc aat ttt cat atg gag aca gga 1755His Gln Thr Asp Val Gly Asn Glu Ala Asn Phe His Met Glu Thr Gly 525 530 535 gtt aag gta caa ggg cca tgg gga ggc aat ggg gga ggt caa ttc ttt 1803Val Lys Val Gln Gly Pro Trp Gly Gly Asn Gly Gly Gly Gln Phe Phe 540 545 550 gat gga ata gtc aca tcc ata aag cag ata acc atg aag tat agc aca 1851Asp Gly Ile Val Thr Ser Ile Lys Gln Ile Thr Met Lys Tyr Ser Thr 555 560 565 gac cca tcc cct tgc ata ttc tac atg gag atg gag tac aac atg aat 1899Asp Pro Ser Pro Cys Ile Phe Tyr Met Glu Met Glu Tyr Asn Met Asn 570 575 580 585 gga aca tca ttt ttt att ggt cat gga gat gcc aat cat ggt tcg aac 1947Gly Thr Ser Phe Phe Ile Gly His Gly Asp Ala Asn His Gly Ser Asn 590 595 600 tct tca act atc aag ata gac gag cct agt gaa tac atc aca aaa gtt 1995Ser Ser Thr Ile Lys Ile Asp Glu Pro Ser Glu Tyr Ile Thr Lys Val 605 610 615 gaa ggg tca tat ggc agc acc cca atg tgg tgt gga ggc aag caa gtg 2043Glu Gly Ser Tyr Gly Ser Thr Pro Met Trp Cys Gly Gly Lys Gln Val 620 625 630 gag agt tta aca tcc tta acc ata cac acc aat gtg aaa gca cat gga 2091Glu Ser Leu Thr Ser Leu Thr Ile His Thr Asn Val Lys Ala His Gly 635 640 645 ccc ttt gga ggg aag tgc aca tcc aag ttc aaa agt gaa tat ggc aga 2139Pro Phe Gly Gly Lys Cys Thr Ser Lys Phe Lys Ser Glu Tyr Gly Arg 650 655 660 665 gtt gtg ggt ttc cat gga aga agt ggt ttg ggg ctt gat tct att ggt 2187Val Val Gly Phe His Gly Arg Ser Gly Leu Gly Leu Asp Ser Ile Gly 670 675 680 tgt ttt aca gta ccc att gaa gtt tgatgtatcc aaaatgaata ccttgggaga 2241Cys Phe Thr Val Pro Ile Glu Val 685 tgattgtcat gtgcatcaat ctttacagta actgtgtaat aatattgtca taggcttgta 2301gaaagttact taccaaaagt aggtatacag tagaaatgga tgtgattcta ttggctaagt 2361agcaatgtag taaattggtg aggtgtagca cttcaatatt tcatcatgaa tactcgtggc 2421agaagaaaac attaaaaaaa actaaaagca ttatcttaca ggaatgttat gagtgcaagg 2481cgttaacc 248928689PRTPhyscomitrella patens 28Met Ala Gly Ile Ser Arg Asp Ile Arg Gly Thr Val Glu Asn Leu Val 1 5 10 15 Arg Leu Thr Ser Asp Ser Asn Ser Thr Asp Pro Leu Lys Leu Asn Val 20 25 30 Leu Gln Cys Gln Leu Val Ala Lys Lys Ala Ala Asp Ser Ser Tyr Thr 35 40 45 Leu Asn Asp Leu Glu Ala Tyr Gln Arg Arg His Glu Lys Ser Ser Asp 50 55 60 Gly Arg Ile Ala Gly Thr Gly Phe Arg Ala Ala Lys Glu Leu Leu Arg 65 70 75 80 Val Leu Lys Asp Ala Glu Ile Leu Ile Arg Glu Cys Cys Ser Glu Lys 85 90 95 Trp Lys Lys Val Val Phe Lys Arg Gly Lys Leu Gln Glu Thr Phe Ala 100 105 110 Lys Ile Ala Tyr Glu Ile Glu Trp His Ser Leu Val Leu Tyr Ser Val 115 120 125 Leu Val Ala Gln Ser Asp Ile Tyr Asp Lys Arg Thr Cys Asp Gly Lys 130 135 140 Leu Arg Ser Ile Asp His Ala Arg Leu Ile Leu Ala Ala Arg Gln Asp 145 150 155 160 Leu Asp Ser Leu Arg Ala Leu Leu Gln Gly Pro His Val Cys Asp Glu 165 170 175 Ile Cys Lys Pro Asp Phe Cys Ser Glu Cys Leu Lys Asp Lys Val Leu 180 185 190 Gln Gln Trp Asp Thr Glu Glu Lys Glu Leu Glu Asp Arg Ser Ser Leu 195 200 205 Ser Gln Ile Met Ser Ser Ile Phe Ser Trp Val Gln Pro Gln Phe Asp 210 215 220 Ser Lys Arg Gln Gln Asn Gly Lys Val Gly Val Ala Glu Val Lys Glu 225 230 235 240 Ile Lys Trp Leu Gly Gln Met Tyr Ala Met Lys Thr Phe Asn Lys Asp 245 250 255 Ala Thr His Glu Ala His Phe Arg Glu Glu Val Ser Asp Met Ala Ala 260 265 270 Leu Asp His Pro Asn Val Val Arg Ile Ile Cys Cys Trp Glu Asp Lys 275 280 285 Asn Tyr Val Ser Ile Leu Met Glu Pro Leu Arg Lys Ser Leu His Asn 290 295 300 Leu Leu Leu Asn Tyr Lys Asp Gly Thr His Ala Pro Ser Ala Pro Thr 305 310 315 320 Pro Phe Thr Ile

Leu Asn Ser Val Asp Ile Met Leu Gln Ile Ala Glu 325 330 335 Gly Val Arg Tyr Val His Ser Lys Asn Phe Thr His Leu Asp Ile Met 340 345 350 Ser Leu Asn Val Leu Val Gln Phe Ala Asp Pro Ile Thr Ser Thr Asp 355 360 365 Val Lys Asp Ser Asp Thr Val Thr Ile Ser Ser Arg Ser Thr Ser Phe 370 375 380 Thr Val Lys Leu Ala Asp Phe Gly Leu Lys Arg Ile Ile Asn Glu Lys 385 390 395 400 Gly Arg Arg Thr Ser Asn Ser Val Lys Thr Ala Trp Thr Ala Pro Glu 405 410 415 Ala Tyr Lys Leu Arg Lys Gly Glu Asp Ser Ala Trp Phe His Pro Arg 420 425 430 Lys Ala Asp Val Tyr Ser Phe Ala Ile Thr Cys Ser Glu Ile Leu Thr 435 440 445 Gly Asp His Pro Phe Ala His Phe Asn Ala Asp Tyr Asn Phe Asp Ala 450 455 460 Val Lys Asp Gly Asp Arg Pro Arg Leu Pro Gly Glu Thr Pro Arg Arg 465 470 475 480 Leu Ala Ala Leu Ile His Arg Cys Trp His Arg Asn Pro Gln Leu Arg 485 490 495 Pro Asp Phe Thr Ala Ile Cys Thr Glu Leu Arg Phe Ile Lys Gly Leu 500 505 510 Ala Leu Arg Gly Asp Ile Lys Ser Leu His Gln Thr Asp Val Gly Asn 515 520 525 Glu Ala Asn Phe His Met Glu Thr Gly Val Lys Val Gln Gly Pro Trp 530 535 540 Gly Gly Asn Gly Gly Gly Gln Phe Phe Asp Gly Ile Val Thr Ser Ile 545 550 555 560 Lys Gln Ile Thr Met Lys Tyr Ser Thr Asp Pro Ser Pro Cys Ile Phe 565 570 575 Tyr Met Glu Met Glu Tyr Asn Met Asn Gly Thr Ser Phe Phe Ile Gly 580 585 590 His Gly Asp Ala Asn His Gly Ser Asn Ser Ser Thr Ile Lys Ile Asp 595 600 605 Glu Pro Ser Glu Tyr Ile Thr Lys Val Glu Gly Ser Tyr Gly Ser Thr 610 615 620 Pro Met Trp Cys Gly Gly Lys Gln Val Glu Ser Leu Thr Ser Leu Thr 625 630 635 640 Ile His Thr Asn Val Lys Ala His Gly Pro Phe Gly Gly Lys Cys Thr 645 650 655 Ser Lys Phe Lys Ser Glu Tyr Gly Arg Val Val Gly Phe His Gly Arg 660 665 670 Ser Gly Leu Gly Leu Asp Ser Ile Gly Cys Phe Thr Val Pro Ile Glu 675 680 685 Val 29831DNAPhyscomitrella patensCDS(20)..(685) 29atcccgggcc gttgtaaag atg ggt act cag tcg ctg att tac agc ttt gtt 52 Met Gly Thr Gln Ser Leu Ile Tyr Ser Phe Val 1 5 10 gcc aga ggc tca acg gtg ctg gcc gag tac act gcc ttt tct ggc aac 100Ala Arg Gly Ser Thr Val Leu Ala Glu Tyr Thr Ala Phe Ser Gly Asn 15 20 25 ttc agc acc att gca gtg caa tgt ctt cag aag ctt cca cca aac aac 148Phe Ser Thr Ile Ala Val Gln Cys Leu Gln Lys Leu Pro Pro Asn Asn 30 35 40 aat aaa ttc act tac acc tgt gat cga cac acc ttc aac tac ctt gtt 196Asn Lys Phe Thr Tyr Thr Cys Asp Arg His Thr Phe Asn Tyr Leu Val 45 50 55 gag gaa ggc tac aca tat ttg gtt gtg gct gat gag gaa ttt ggg agg 244Glu Glu Gly Tyr Thr Tyr Leu Val Val Ala Asp Glu Glu Phe Gly Arg 60 65 70 75 caa att ccg ttt gct ttc ctt gag cga gtg aag gag gac ttt aag cgg 292Gln Ile Pro Phe Ala Phe Leu Glu Arg Val Lys Glu Asp Phe Lys Arg 80 85 90 cgt tat gca gga gga aag gcc gac tcg gcc atc gcc aac agt tta gat 340Arg Tyr Ala Gly Gly Lys Ala Asp Ser Ala Ile Ala Asn Ser Leu Asp 95 100 105 aaa gaa ttc ggt ccg aaa ctg aag gac cac atg cag tac tgc gtc gat 388Lys Glu Phe Gly Pro Lys Leu Lys Asp His Met Gln Tyr Cys Val Asp 110 115 120 cac cct gat gaa atg aac aaa att tcg aag att aag tcc caa gtt gcg 436His Pro Asp Glu Met Asn Lys Ile Ser Lys Ile Lys Ser Gln Val Ala 125 130 135 gaa gtc aag gga atc atg atg gac aat atc gag aag gtg ctt gat cgt 484Glu Val Lys Gly Ile Met Met Asp Asn Ile Glu Lys Val Leu Asp Arg 140 145 150 155 gga gag aag att gag ctt ctt gtt gat aag aca gag aac ttg cgt ttc 532Gly Glu Lys Ile Glu Leu Leu Val Asp Lys Thr Glu Asn Leu Arg Phe 160 165 170 cag gct gac aac ttt cag cga caa ggc aag caa ctg cgt cgc aag atg 580Gln Ala Asp Asn Phe Gln Arg Gln Gly Lys Gln Leu Arg Arg Lys Met 175 180 185 tgg ttc cag aac atg aaa gtg aag ctt ata gtt ctt gcc atc att atc 628Trp Phe Gln Asn Met Lys Val Lys Leu Ile Val Leu Ala Ile Ile Ile 190 195 200 gtc atc atc atc atc att tgg ctt tcc att tgc cgt gga ttc act tgc 676Val Ile Ile Ile Ile Ile Trp Leu Ser Ile Cys Arg Gly Phe Thr Cys 205 210 215 agc aat cgc taagtgtata tactgactgg aggtggaaag cagaagcctg 725Ser Asn Arg 220 cactaatttt tcattgtttt tgtttttcgc tttctgccca aatcttcagg tagtgaatca 785tgaaatttga gtctgtggcc tctgtcaggg agttgtgtga gctcgc 83130222PRTPhyscomitrella patens 30Met Gly Thr Gln Ser Leu Ile Tyr Ser Phe Val Ala Arg Gly Ser Thr 1 5 10 15 Val Leu Ala Glu Tyr Thr Ala Phe Ser Gly Asn Phe Ser Thr Ile Ala 20 25 30 Val Gln Cys Leu Gln Lys Leu Pro Pro Asn Asn Asn Lys Phe Thr Tyr 35 40 45 Thr Cys Asp Arg His Thr Phe Asn Tyr Leu Val Glu Glu Gly Tyr Thr 50 55 60 Tyr Leu Val Val Ala Asp Glu Glu Phe Gly Arg Gln Ile Pro Phe Ala 65 70 75 80 Phe Leu Glu Arg Val Lys Glu Asp Phe Lys Arg Arg Tyr Ala Gly Gly 85 90 95 Lys Ala Asp Ser Ala Ile Ala Asn Ser Leu Asp Lys Glu Phe Gly Pro 100 105 110 Lys Leu Lys Asp His Met Gln Tyr Cys Val Asp His Pro Asp Glu Met 115 120 125 Asn Lys Ile Ser Lys Ile Lys Ser Gln Val Ala Glu Val Lys Gly Ile 130 135 140 Met Met Asp Asn Ile Glu Lys Val Leu Asp Arg Gly Glu Lys Ile Glu 145 150 155 160 Leu Leu Val Asp Lys Thr Glu Asn Leu Arg Phe Gln Ala Asp Asn Phe 165 170 175 Gln Arg Gln Gly Lys Gln Leu Arg Arg Lys Met Trp Phe Gln Asn Met 180 185 190 Lys Val Lys Leu Ile Val Leu Ala Ile Ile Ile Val Ile Ile Ile Ile 195 200 205 Ile Trp Leu Ser Ile Cys Arg Gly Phe Thr Cys Ser Asn Arg 210 215 220 311342DNAPhyscomitrella patensCDS(43)..(588) 31atcccgggtg tgccagtgac cgagcatacg cacggtgatc tt atg tta tct ttg 54 Met Leu Ser Leu 1 cat gcc cgg cgg ctt tgc ttg aat tca ccg tcg aat tac atg gtt gaa 102His Ala Arg Arg Leu Cys Leu Asn Ser Pro Ser Asn Tyr Met Val Glu 5 10 15 20 ttg ggg aga gtg gag aag gta ctt ggc ctg agg gca ggt gcc gtg aag 150Leu Gly Arg Val Glu Lys Val Leu Gly Leu Arg Ala Gly Ala Val Lys 25 30 35 atc ttt cta gag aag ttt gcc gct atg aat cca act tct tgc ggt acc 198Ile Phe Leu Glu Lys Phe Ala Ala Met Asn Pro Thr Ser Cys Gly Thr 40 45 50 gtc agc ttg aac caa ttt gtc aaa tgg cat cat atg ccg aaa tgc tgg 246Val Ser Leu Asn Gln Phe Val Lys Trp His His Met Pro Lys Cys Trp 55 60 65 atg tcg aag aag ata ttt gat ctt ttc gac aaa tcg gga cag ggc ttc 294Met Ser Lys Lys Ile Phe Asp Leu Phe Asp Lys Ser Gly Gln Gly Phe 70 75 80 acg act ttt aga gag ttc gtg gcg gta atg gga tca atc act aaa agc 342Thr Thr Phe Arg Glu Phe Val Ala Val Met Gly Ser Ile Thr Lys Ser 85 90 95 100 aag gag ttt aag agt caa atg aaa gca gct tac gat gca tgt aac ctt 390Lys Glu Phe Lys Ser Gln Met Lys Ala Ala Tyr Asp Ala Cys Asn Leu 105 110 115 caa aac agt gac tgc atc tca caa ctg gag ctg gaa aaa tgc ctg aag 438Gln Asn Ser Asp Cys Ile Ser Gln Leu Glu Leu Glu Lys Cys Leu Lys 120 125 130 tta agt atg cca aca att agc tct gca tac gtg agg gcg tgg ttc agt 486Leu Ser Met Pro Thr Ile Ser Ser Ala Tyr Val Arg Ala Trp Phe Ser 135 140 145 aag att tct cag cac gat gat ggg gcc ata agc tgg gag gat ttc caa 534Lys Ile Ser Gln His Asp Asp Gly Ala Ile Ser Trp Glu Asp Phe Gln 150 155 160 gtc ttc tta gag acg aac cca gag cta ttg ccc att ttc atg gtg gga 582Val Phe Leu Glu Thr Asn Pro Glu Leu Leu Pro Ile Phe Met Val Gly 165 170 175 180 act ttt taacttcgag ccagcctgtg agatgggtta tctcaaagtg gactttctct 638Thr Phe ctgtcttggc ttgaactcta ctttgacata gcccacttca aaggtaactt gaatacactc 698aaaacgacgt caagttcgct gtgtaccggc cctatcaaca cccaggcagc ccagattgta 758ctggtaatca gggcctaatt cttgatgtca tcgctgcctg tagaatgcaa agacgtgaac 818agggttcacc agtgagggtg tttgattcct accgaattat cgggaccttg attgtgaggt 878tcgtgacaaa tccggaactt gacgttgatg gtatatttcg agattcgctt gagagtcgac 938tgtcaagttt gcgtagtgta ctgaagaaca ttgtttatca ttttggaatt ctactgagca 998gaccgcagtg tctggtgagt tgcaatgctt gggaaacgga aagctccgtt gaattcaacg 1058tcctacactc gaagaatcaa gctgcagctt tgcgagttga acacatcgtg acagtctatt 1118acggtgcttc attatgtgca gtatcggtgt tcaacaaatt agcaccaatc tagaacagca 1178ttccgttgca aaagcagaga acaaagccta tgtggtcatt cttcacagag gactttggtg 1238tgcacctctt gtaaacccat tacatttctc cttgatgtag attgaaccat gtttttatga 1298atttaaaatg ccacggtgac tggtgttgtg tagcccgagc tcgc 134232182PRTPhyscomitrella patens 32Met Leu Ser Leu His Ala Arg Arg Leu Cys Leu Asn Ser Pro Ser Asn 1 5 10 15 Tyr Met Val Glu Leu Gly Arg Val Glu Lys Val Leu Gly Leu Arg Ala 20 25 30 Gly Ala Val Lys Ile Phe Leu Glu Lys Phe Ala Ala Met Asn Pro Thr 35 40 45 Ser Cys Gly Thr Val Ser Leu Asn Gln Phe Val Lys Trp His His Met 50 55 60 Pro Lys Cys Trp Met Ser Lys Lys Ile Phe Asp Leu Phe Asp Lys Ser 65 70 75 80 Gly Gln Gly Phe Thr Thr Phe Arg Glu Phe Val Ala Val Met Gly Ser 85 90 95 Ile Thr Lys Ser Lys Glu Phe Lys Ser Gln Met Lys Ala Ala Tyr Asp 100 105 110 Ala Cys Asn Leu Gln Asn Ser Asp Cys Ile Ser Gln Leu Glu Leu Glu 115 120 125 Lys Cys Leu Lys Leu Ser Met Pro Thr Ile Ser Ser Ala Tyr Val Arg 130 135 140 Ala Trp Phe Ser Lys Ile Ser Gln His Asp Asp Gly Ala Ile Ser Trp 145 150 155 160 Glu Asp Phe Gln Val Phe Leu Glu Thr Asn Pro Glu Leu Leu Pro Ile 165 170 175 Phe Met Val Gly Thr Phe 180 33804DNAPhyscomitrella patensCDS(16)..(732) 33atcccgggcg gaaag atg acc gaa acg acg ggc agc ggt gca ttg tca cag 51 Met Thr Glu Thr Thr Gly Ser Gly Ala Leu Ser Gln 1 5 10 gcg gcg gat tgt tta ccg ttg act tat caa agg cca gta cgg gat gac 99Ala Ala Asp Cys Leu Pro Leu Thr Tyr Gln Arg Pro Val Arg Asp Asp 15 20 25 ttg gaa act cat ctt cca aaa cct tac cta gcg aga gca ttg gta gct 147Leu Glu Thr His Leu Pro Lys Pro Tyr Leu Ala Arg Ala Leu Val Ala 30 35 40 cca gat aca gaa cat cca aac ggg acg tta ggg cac agg cat aat ggc 195Pro Asp Thr Glu His Pro Asn Gly Thr Leu Gly His Arg His Asn Gly 45 50 55 60 atg act gtt ctt cag cag cat att gct ttc ttt gat caa aat ggt gac 243Met Thr Val Leu Gln Gln His Ile Ala Phe Phe Asp Gln Asn Gly Asp 65 70 75 gga atc att tac cca tgg gag acc tat gct gga ctg cgt gaa ata gga 291Gly Ile Ile Tyr Pro Trp Glu Thr Tyr Ala Gly Leu Arg Glu Ile Gly 80 85 90 ttc aat gtc ata tgg tcc gca atg gtt gcc ttt ata atc aat gtg gtg 339Phe Asn Val Ile Trp Ser Ala Met Val Ala Phe Ile Ile Asn Val Val 95 100 105 atg agc tat gca tcc ctc cct ggg tgg ttg cct tcg ccc ttt ttg ccc 387Met Ser Tyr Ala Ser Leu Pro Gly Trp Leu Pro Ser Pro Phe Leu Pro 110 115 120 ata tat atc tac aat ata cac aag gca aaa cat gga agc gac tcg ggg 435Ile Tyr Ile Tyr Asn Ile His Lys Ala Lys His Gly Ser Asp Ser Gly 125 130 135 140 gct tat gat acc gag gga aga tat gtg ccg gtg tac ttt gag aac gtg 483Ala Tyr Asp Thr Glu Gly Arg Tyr Val Pro Val Tyr Phe Glu Asn Val 145 150 155 ttt agc aag tat gct aga aca gtg cct gat aag ctc aca ctc gga gag 531Phe Ser Lys Tyr Ala Arg Thr Val Pro Asp Lys Leu Thr Leu Gly Glu 160 165 170 att tgg agc atg acc gaa ggg aat cga gta gct tat gat ttc ttt gga 579Ile Trp Ser Met Thr Glu Gly Asn Arg Val Ala Tyr Asp Phe Phe Gly 175 180 185 tgg gct gcg gct aag gga gaa tgg ata ctt ttg tat atg ctt gct aag 627Trp Ala Ala Ala Lys Gly Glu Trp Ile Leu Leu Tyr Met Leu Ala Lys 190 195 200 gac gag gaa ggc atg ctg tca aag gag gcg tgt agg cgt tgt ttt gac 675Asp Glu Glu Gly Met Leu Ser Lys Glu Ala Cys Arg Arg Cys Phe Asp 205 210 215 220 ggt agc ttg ttt gag tat tgc gcc aag atg aac agg atg caa cac gag 723Gly Ser Leu Phe Glu Tyr Cys Ala Lys Met Asn Arg Met Gln His Glu 225 230 235 aag gcg tat tgagcattat tagattttta gaaaccgttt gtgttgataa 772Lys Ala Tyr tgtagagtat gttgtttaga tcgggagctc gc 80434239PRTPhyscomitrella patens 34Met Thr Glu Thr Thr Gly Ser Gly Ala Leu Ser Gln Ala Ala Asp Cys 1 5 10 15 Leu Pro Leu Thr Tyr Gln Arg Pro Val Arg Asp Asp Leu Glu Thr His 20 25 30 Leu Pro Lys Pro Tyr Leu Ala Arg Ala Leu Val Ala Pro Asp Thr Glu 35 40 45 His Pro Asn Gly Thr Leu Gly His Arg His Asn Gly Met Thr Val Leu 50 55 60 Gln Gln His Ile Ala Phe Phe Asp Gln Asn Gly Asp Gly Ile Ile Tyr 65 70 75 80 Pro Trp Glu Thr Tyr Ala Gly Leu Arg Glu Ile Gly Phe Asn Val Ile 85 90 95 Trp Ser Ala Met Val Ala Phe Ile Ile Asn Val Val Met Ser Tyr Ala 100 105 110 Ser Leu Pro Gly Trp Leu Pro Ser Pro Phe Leu Pro Ile Tyr Ile Tyr 115 120 125 Asn Ile His Lys Ala Lys His Gly Ser Asp Ser Gly Ala Tyr Asp Thr 130 135 140 Glu Gly Arg Tyr Val Pro Val Tyr Phe Glu Asn Val Phe Ser Lys Tyr 145 150 155 160 Ala Arg Thr Val Pro Asp Lys Leu Thr Leu Gly Glu Ile Trp Ser Met 165 170 175 Thr Glu Gly Asn Arg Val Ala Tyr Asp Phe Phe Gly Trp Ala Ala Ala

180 185 190 Lys Gly Glu Trp Ile Leu Leu Tyr Met Leu Ala Lys Asp Glu Glu Gly 195 200 205 Met Leu Ser Lys Glu Ala Cys Arg Arg Cys Phe Asp Gly Ser Leu Phe 210 215 220 Glu Tyr Cys Ala Lys Met Asn Arg Met Gln His Glu Lys Ala Tyr 225 230 235 351562DNAPhyscomitrella patensCDS(166)..(1458) 35gcgttaacgg gtgtgagcga cgagcttcat gagtagcact tcctgagatg aattgatcct 60cccgctgggt cgcacccgga tgttcgcagt gctggagaat gtatacgtaa cgaggatcat 120tgcccacttt cgtccggagc gctttgctga gcctcgtcgt ccact atg cct gtt aag 177 Met Pro Val Lys 1 gat cgc att tcg tac ttt tac gat ggg gac gtg ggt agt gtg tac tat 225Asp Arg Ile Ser Tyr Phe Tyr Asp Gly Asp Val Gly Ser Val Tyr Tyr 5 10 15 20 ggg cca aac cat cca atg aag ccc cat cgg ttg tgt atg aca aac agt 273Gly Pro Asn His Pro Met Lys Pro His Arg Leu Cys Met Thr Asn Ser 25 30 35 ctc gtc ctt gct tat gga ctt cac aac aag atg gag att tat cga ccc 321Leu Val Leu Ala Tyr Gly Leu His Asn Lys Met Glu Ile Tyr Arg Pro 40 45 50 cac aaa gcc tac ccg gtg gaa ctc gcg cag ttt cac tct gtt gac tat 369His Lys Ala Tyr Pro Val Glu Leu Ala Gln Phe His Ser Val Asp Tyr 55 60 65 gtt gag ttt ctc ggc cga att act cct gaa tct cag gaa aag tat gca 417Val Glu Phe Leu Gly Arg Ile Thr Pro Glu Ser Gln Glu Lys Tyr Ala 70 75 80 gcg gag ttg ata aga tat aac atg ggg gag gat tgc cct gtt ttt gac 465Ala Glu Leu Ile Arg Tyr Asn Met Gly Glu Asp Cys Pro Val Phe Asp 85 90 95 100 aac ctt ttt gaa ttt tgt caa att tat gct ggg ggt act att gat gcc 513Asn Leu Phe Glu Phe Cys Gln Ile Tyr Ala Gly Gly Thr Ile Asp Ala 105 110 115 gcg cat cgt ctg aac cat ggc tta tgt gac ata gcc atc aac tgg gct 561Ala His Arg Leu Asn His Gly Leu Cys Asp Ile Ala Ile Asn Trp Ala 120 125 130 gga ggt tta cat cat gca aag aag tgt gaa gcc tct gga ttt tgt tac 609Gly Gly Leu His His Ala Lys Lys Cys Glu Ala Ser Gly Phe Cys Tyr 135 140 145 gtg aat gac cta gtt ttg ggc att tta gaa ctt ttg aag tat cac gct 657Val Asn Asp Leu Val Leu Gly Ile Leu Glu Leu Leu Lys Tyr His Ala 150 155 160 cgc gtg cta tat att gac ata gat att cac cat gga gac gga gta gaa 705Arg Val Leu Tyr Ile Asp Ile Asp Ile His His Gly Asp Gly Val Glu 165 170 175 180 gaa gcg ttt tat ctt act gac aga gta atg acc gtt agt ttt cat aaa 753Glu Ala Phe Tyr Leu Thr Asp Arg Val Met Thr Val Ser Phe His Lys 185 190 195 ttt gga gac tac ttc ttc cca ggc act ggg gat gta aag gac gtt gga 801Phe Gly Asp Tyr Phe Phe Pro Gly Thr Gly Asp Val Lys Asp Val Gly 200 205 210 gag aga gaa gga aaa tat tat gca atc aac gtg ccg cta aaa gat ggc 849Glu Arg Glu Gly Lys Tyr Tyr Ala Ile Asn Val Pro Leu Lys Asp Gly 215 220 225 att gat gac gca aat ttc ata cgg atg ttt cgc gtg gta atc caa aag 897Ile Asp Asp Ala Asn Phe Ile Arg Met Phe Arg Val Val Ile Gln Lys 230 235 240 gtt gtg gaa gtt tat caa cct ggt gcc att gtt ctg caa tgt gga gct 945Val Val Glu Val Tyr Gln Pro Gly Ala Ile Val Leu Gln Cys Gly Ala 245 250 255 260 gac tca ctt gca ggg gat cgt tta ggc tgc ttc aat ctt tcc att gat 993Asp Ser Leu Ala Gly Asp Arg Leu Gly Cys Phe Asn Leu Ser Ile Asp 265 270 275 gga cac tcg gaa tgt gtg aag ttt gtg aag aag ttc aac ata ccc ctt 1041Gly His Ser Glu Cys Val Lys Phe Val Lys Lys Phe Asn Ile Pro Leu 280 285 290 ctg gtg aca ggt ggg gga gga tac acc aag gag aat gtc gca cgc tgt 1089Leu Val Thr Gly Gly Gly Gly Tyr Thr Lys Glu Asn Val Ala Arg Cys 295 300 305 tgg aca gtg gag act ggt gtt ctt gtg gat act gag ctg ccg aat gaa 1137Trp Thr Val Glu Thr Gly Val Leu Val Asp Thr Glu Leu Pro Asn Glu 310 315 320 att cct gac aat gac tac ctg aag tat ttc aaa cca gat tgc act ttg 1185Ile Pro Asp Asn Asp Tyr Leu Lys Tyr Phe Lys Pro Asp Cys Thr Leu 325 330 335 340 aag acc aca tca gga aat cac atg gaa aac ttg aac ggt aag acc tac 1233Lys Thr Thr Ser Gly Asn His Met Glu Asn Leu Asn Gly Lys Thr Tyr 345 350 355 ctg agc act atc aag cag cag gtt atg gag aac tta cgg aga att gct 1281Leu Ser Thr Ile Lys Gln Gln Val Met Glu Asn Leu Arg Arg Ile Ala 360 365 370 cat gca cct agt gtt caa atg cac gag gta cct ccg gac act tat ata 1329His Ala Pro Ser Val Gln Met His Glu Val Pro Pro Asp Thr Tyr Ile 375 380 385 cca gag ttt gat gag gat gaa ttg aat cct gac gag cgc atg gac caa 1377Pro Glu Phe Asp Glu Asp Glu Leu Asn Pro Asp Glu Arg Met Asp Gln 390 395 400 cac aca cag gac aag cac atc caa agg gag gag gag tat tat gaa gat 1425His Thr Gln Asp Lys His Ile Gln Arg Glu Glu Glu Tyr Tyr Glu Asp 405 410 415 420 gac aac gac aac gac cat gac atg gat gac tca tgactgttta ttagatgttt 1478Asp Asn Asp Asn Asp His Asp Met Asp Asp Ser 425 430 ttagaagata actgaaaaca tgtcctcatt tgtacactag attttacccc tactaacaca 1538ttgaatgaaa gagttggagc tcgc 156236431PRTPhyscomitrella patens 36Met Pro Val Lys Asp Arg Ile Ser Tyr Phe Tyr Asp Gly Asp Val Gly 1 5 10 15 Ser Val Tyr Tyr Gly Pro Asn His Pro Met Lys Pro His Arg Leu Cys 20 25 30 Met Thr Asn Ser Leu Val Leu Ala Tyr Gly Leu His Asn Lys Met Glu 35 40 45 Ile Tyr Arg Pro His Lys Ala Tyr Pro Val Glu Leu Ala Gln Phe His 50 55 60 Ser Val Asp Tyr Val Glu Phe Leu Gly Arg Ile Thr Pro Glu Ser Gln 65 70 75 80 Glu Lys Tyr Ala Ala Glu Leu Ile Arg Tyr Asn Met Gly Glu Asp Cys 85 90 95 Pro Val Phe Asp Asn Leu Phe Glu Phe Cys Gln Ile Tyr Ala Gly Gly 100 105 110 Thr Ile Asp Ala Ala His Arg Leu Asn His Gly Leu Cys Asp Ile Ala 115 120 125 Ile Asn Trp Ala Gly Gly Leu His His Ala Lys Lys Cys Glu Ala Ser 130 135 140 Gly Phe Cys Tyr Val Asn Asp Leu Val Leu Gly Ile Leu Glu Leu Leu 145 150 155 160 Lys Tyr His Ala Arg Val Leu Tyr Ile Asp Ile Asp Ile His His Gly 165 170 175 Asp Gly Val Glu Glu Ala Phe Tyr Leu Thr Asp Arg Val Met Thr Val 180 185 190 Ser Phe His Lys Phe Gly Asp Tyr Phe Phe Pro Gly Thr Gly Asp Val 195 200 205 Lys Asp Val Gly Glu Arg Glu Gly Lys Tyr Tyr Ala Ile Asn Val Pro 210 215 220 Leu Lys Asp Gly Ile Asp Asp Ala Asn Phe Ile Arg Met Phe Arg Val 225 230 235 240 Val Ile Gln Lys Val Val Glu Val Tyr Gln Pro Gly Ala Ile Val Leu 245 250 255 Gln Cys Gly Ala Asp Ser Leu Ala Gly Asp Arg Leu Gly Cys Phe Asn 260 265 270 Leu Ser Ile Asp Gly His Ser Glu Cys Val Lys Phe Val Lys Lys Phe 275 280 285 Asn Ile Pro Leu Leu Val Thr Gly Gly Gly Gly Tyr Thr Lys Glu Asn 290 295 300 Val Ala Arg Cys Trp Thr Val Glu Thr Gly Val Leu Val Asp Thr Glu 305 310 315 320 Leu Pro Asn Glu Ile Pro Asp Asn Asp Tyr Leu Lys Tyr Phe Lys Pro 325 330 335 Asp Cys Thr Leu Lys Thr Thr Ser Gly Asn His Met Glu Asn Leu Asn 340 345 350 Gly Lys Thr Tyr Leu Ser Thr Ile Lys Gln Gln Val Met Glu Asn Leu 355 360 365 Arg Arg Ile Ala His Ala Pro Ser Val Gln Met His Glu Val Pro Pro 370 375 380 Asp Thr Tyr Ile Pro Glu Phe Asp Glu Asp Glu Leu Asn Pro Asp Glu 385 390 395 400 Arg Met Asp Gln His Thr Gln Asp Lys His Ile Gln Arg Glu Glu Glu 405 410 415 Tyr Tyr Glu Asp Asp Asn Asp Asn Asp His Asp Met Asp Asp Ser 420 425 430 371544DNABrassica napusCDS(84)..(1361) 37aggaatcgtc tcacatctat tgaagccata attgttgtta acaggtcgat ctcggttgta 60gttttttttt tttcttagaa gag atg cgg tcc aag gac aaa atc tcc tac ttt 113 Met Arg Ser Lys Asp Lys Ile Ser Tyr Phe 1 5 10 tac gat gga gat gta ggg agc gtt tat ttt ggt ccg aat cac cca atg 161Tyr Asp Gly Asp Val Gly Ser Val Tyr Phe Gly Pro Asn His Pro Met 15 20 25 aaa cct cac agg ctt tgt atg acc cat cat ctt atc ctt gca tat ggc 209Lys Pro His Arg Leu Cys Met Thr His His Leu Ile Leu Ala Tyr Gly 30 35 40 ctc cat agc aag atg gaa gtt tat cgt cca cac aag gca tac cct atc 257Leu His Ser Lys Met Glu Val Tyr Arg Pro His Lys Ala Tyr Pro Ile 45 50 55 gag atg gcc cag ttc cat tct cca gac tat gtc gag ttc ctg caa cga 305Glu Met Ala Gln Phe His Ser Pro Asp Tyr Val Glu Phe Leu Gln Arg 60 65 70 atc aac cca gaa aat aag gat ttg ttt ccc aac gaa atg gct aga tat 353Ile Asn Pro Glu Asn Lys Asp Leu Phe Pro Asn Glu Met Ala Arg Tyr 75 80 85 90 aat tta gga gag gat tgt cct gtc ttt gag gat atg ttc gag ttt tgt 401Asn Leu Gly Glu Asp Cys Pro Val Phe Glu Asp Met Phe Glu Phe Cys 95 100 105 caa att tat gcg ggt gca acc ata gat gct gca cgc aga tta aac aac 449Gln Ile Tyr Ala Gly Ala Thr Ile Asp Ala Ala Arg Arg Leu Asn Asn 110 115 120 aaa ctc tgt gac att gcg ata aac tgg gcg ggc ggg ttg cac cat gct 497Lys Leu Cys Asp Ile Ala Ile Asn Trp Ala Gly Gly Leu His His Ala 125 130 135 aaa aaa tgc gat gca tct ggt ttt tgt tac atc aac gat ctc gta cta 545Lys Lys Cys Asp Ala Ser Gly Phe Cys Tyr Ile Asn Asp Leu Val Leu 140 145 150 gga atc ctc gag ctg ttg aaa cac cat cct cgt gtg ctc tac att gat 593Gly Ile Leu Glu Leu Leu Lys His His Pro Arg Val Leu Tyr Ile Asp 155 160 165 170 ata gac gtt cac cac ggt gat gga gtt gaa gag gct ttt tac ttt act 641Ile Asp Val His His Gly Asp Gly Val Glu Glu Ala Phe Tyr Phe Thr 175 180 185 gac aga gtg atg act gtt agt ttt cac aag ttt ggg gat aag ttc ttt 689Asp Arg Val Met Thr Val Ser Phe His Lys Phe Gly Asp Lys Phe Phe 190 195 200 cca ggg acc ggc gat gtt aag gaa ata gga gaa agg gaa ggg aag ttt 737Pro Gly Thr Gly Asp Val Lys Glu Ile Gly Glu Arg Glu Gly Lys Phe 205 210 215 tac gcc ata aat gtt ccg ctc agg gat ggg att gat gac agt agt ttc 785Tyr Ala Ile Asn Val Pro Leu Arg Asp Gly Ile Asp Asp Ser Ser Phe 220 225 230 aac cgt ctg ttc agg gca ata att tca aag gtg gtt gag ata tat cag 833Asn Arg Leu Phe Arg Ala Ile Ile Ser Lys Val Val Glu Ile Tyr Gln 235 240 245 250 cca ggt gca ata gta ctt cag tgt gga gca gat tca cta gca agg gat 881Pro Gly Ala Ile Val Leu Gln Cys Gly Ala Asp Ser Leu Ala Arg Asp 255 260 265 cga cta gga tgc ttt aat ctc tct att gat gga cat gct gaa tgt gtt 929Arg Leu Gly Cys Phe Asn Leu Ser Ile Asp Gly His Ala Glu Cys Val 270 275 280 aaa ttc gtc aag aaa ttc aat att cct ttg ctg gtg act gga ggt gga 977Lys Phe Val Lys Lys Phe Asn Ile Pro Leu Leu Val Thr Gly Gly Gly 285 290 295 ggg tac aca aag gag aac gta gct cgg tgt tgg acc gtt gag act ggc 1025Gly Tyr Thr Lys Glu Asn Val Ala Arg Cys Trp Thr Val Glu Thr Gly 300 305 310 att ctt ttg gac aca gaa ctt cct aat gag att cct gat aat gat tat 1073Ile Leu Leu Asp Thr Glu Leu Pro Asn Glu Ile Pro Asp Asn Asp Tyr 315 320 325 330 ata aag tat ttt ggg ccg gat tat tca ttg aag att cct ggt ggt cac 1121Ile Lys Tyr Phe Gly Pro Asp Tyr Ser Leu Lys Ile Pro Gly Gly His 335 340 345 att gag aat cta aat acg aaa tcg tat atc agt acg ata aaa gca cag 1169Ile Glu Asn Leu Asn Thr Lys Ser Tyr Ile Ser Thr Ile Lys Ala Gln 350 355 360 att ttg gat aat ttg aga tac atc cag cac gct cca agc gtg cag atg 1217Ile Leu Asp Asn Leu Arg Tyr Ile Gln His Ala Pro Ser Val Gln Met 365 370 375 cag gag gtt cca ccg gat ttc tac ata ccg gat ttt gat gaa gac gaa 1265Gln Glu Val Pro Pro Asp Phe Tyr Ile Pro Asp Phe Asp Glu Asp Glu 380 385 390 cga aat cca gat gtg cgt gtg gac cag cgt tcg cgg gat aag cag att 1313Arg Asn Pro Asp Val Arg Val Asp Gln Arg Ser Arg Asp Lys Gln Ile 395 400 405 410 cag agg gac gat gaa tat ttc gat ggt gac aag gat aac gat gcg tcg 1361Gln Arg Asp Asp Glu Tyr Phe Asp Gly Asp Lys Asp Asn Asp Ala Ser 415 420 425 tagcatagat tattattagc gcagaagact taagacaaaa ccaaagttgt gtttgggaga 1421tttgttataa acttataatg ataacatttt aacggcttgt agaaaattct atttatctgg 1481gcaccaaaac ccactcatga ttcttaaatc gttcgtcttt tctccaaaaa aaaaaaaaaa 1541aaa 154438426PRTBrassica napus 38Met Arg Ser Lys Asp Lys Ile Ser Tyr Phe Tyr Asp Gly Asp Val Gly 1 5 10 15 Ser Val Tyr Phe Gly Pro Asn His Pro Met Lys Pro His Arg Leu Cys 20 25 30 Met Thr His His Leu Ile Leu Ala Tyr Gly Leu His Ser Lys Met Glu 35 40 45 Val Tyr Arg Pro His Lys Ala Tyr Pro Ile Glu Met Ala Gln Phe His 50 55 60 Ser Pro Asp Tyr Val Glu Phe Leu Gln Arg Ile Asn Pro Glu Asn Lys 65 70 75 80 Asp Leu Phe Pro Asn Glu Met Ala Arg Tyr Asn Leu Gly Glu Asp Cys 85 90 95 Pro Val Phe Glu Asp Met Phe Glu Phe Cys Gln Ile Tyr Ala Gly Ala 100 105 110 Thr Ile Asp Ala Ala Arg Arg Leu Asn Asn Lys Leu Cys Asp Ile Ala 115 120 125 Ile Asn Trp Ala Gly Gly Leu His His Ala Lys Lys Cys Asp Ala Ser 130 135 140 Gly Phe Cys Tyr Ile Asn Asp Leu Val Leu Gly Ile Leu Glu Leu Leu 145 150 155 160 Lys His His Pro Arg Val Leu Tyr Ile Asp Ile Asp Val His His Gly 165 170 175 Asp Gly Val Glu Glu Ala Phe Tyr Phe Thr Asp Arg Val Met Thr Val 180 185 190 Ser Phe His Lys Phe Gly Asp Lys Phe Phe Pro Gly Thr Gly Asp Val 195

200 205 Lys Glu Ile Gly Glu Arg Glu Gly Lys Phe Tyr Ala Ile Asn Val Pro 210 215 220 Leu Arg Asp Gly Ile Asp Asp Ser Ser Phe Asn Arg Leu Phe Arg Ala 225 230 235 240 Ile Ile Ser Lys Val Val Glu Ile Tyr Gln Pro Gly Ala Ile Val Leu 245 250 255 Gln Cys Gly Ala Asp Ser Leu Ala Arg Asp Arg Leu Gly Cys Phe Asn 260 265 270 Leu Ser Ile Asp Gly His Ala Glu Cys Val Lys Phe Val Lys Lys Phe 275 280 285 Asn Ile Pro Leu Leu Val Thr Gly Gly Gly Gly Tyr Thr Lys Glu Asn 290 295 300 Val Ala Arg Cys Trp Thr Val Glu Thr Gly Ile Leu Leu Asp Thr Glu 305 310 315 320 Leu Pro Asn Glu Ile Pro Asp Asn Asp Tyr Ile Lys Tyr Phe Gly Pro 325 330 335 Asp Tyr Ser Leu Lys Ile Pro Gly Gly His Ile Glu Asn Leu Asn Thr 340 345 350 Lys Ser Tyr Ile Ser Thr Ile Lys Ala Gln Ile Leu Asp Asn Leu Arg 355 360 365 Tyr Ile Gln His Ala Pro Ser Val Gln Met Gln Glu Val Pro Pro Asp 370 375 380 Phe Tyr Ile Pro Asp Phe Asp Glu Asp Glu Arg Asn Pro Asp Val Arg 385 390 395 400 Val Asp Gln Arg Ser Arg Asp Lys Gln Ile Gln Arg Asp Asp Glu Tyr 405 410 415 Phe Asp Gly Asp Lys Asp Asn Asp Ala Ser 420 425 391594DNABrassica napusCDS(18)..(1427) 39gagaaaggaa gggcgac atg gag aca gac gag agc ggc gtc tct tta gcg 50 Met Glu Thr Asp Glu Ser Gly Val Ser Leu Ala 1 5 10 tca ggg ccc gac ggt cgt aag cga cga gtc agc tac ttc tac gag cca 98Ser Gly Pro Asp Gly Arg Lys Arg Arg Val Ser Tyr Phe Tyr Glu Pro 15 20 25 acg atc ggt aac tac tac tac ggt caa ggc cac ccc atg aag cct cac 146Thr Ile Gly Asn Tyr Tyr Tyr Gly Gln Gly His Pro Met Lys Pro His 30 35 40 cgg atc cgt atg gct cat agt cta atc gtc cac tac aac ctc cac cgc 194Arg Ile Arg Met Ala His Ser Leu Ile Val His Tyr Asn Leu His Arg 45 50 55 cgc ctc gag atc agc cgc cct tac ctc gcc gac gct gcc gac atc ggt 242Arg Leu Glu Ile Ser Arg Pro Tyr Leu Ala Asp Ala Ala Asp Ile Gly 60 65 70 75 cgc ttc cac tct ccc gag tac gtc gat ttc ctc cgc tcc gtt tcg ccg 290Arg Phe His Ser Pro Glu Tyr Val Asp Phe Leu Arg Ser Val Ser Pro 80 85 90 gag tcc gtc ggc gat tcg tcc gcg cgt aac cta agg cga ttc aat gtc 338Glu Ser Val Gly Asp Ser Ser Ala Arg Asn Leu Arg Arg Phe Asn Val 95 100 105 ggc gag gat tgt ccc gtc ttc gac ggt ctt ttc gag ttt tgc cgc gct 386Gly Glu Asp Cys Pro Val Phe Asp Gly Leu Phe Glu Phe Cys Arg Ala 110 115 120 tcc gcc gga ggt tcg atc ggc gcc gcc gtt aaa ttg aac cgg cag gac 434Ser Ala Gly Gly Ser Ile Gly Ala Ala Val Lys Leu Asn Arg Gln Asp 125 130 135 gcg gat atc gcc atc aat tgg ggc ggt ggg ctt cac cac gct aag aag 482Ala Asp Ile Ala Ile Asn Trp Gly Gly Gly Leu His His Ala Lys Lys 140 145 150 155 agc gag gcg tct ggg ttt tgc tac gta aac gac atc gtt ttg ggg att 530Ser Glu Ala Ser Gly Phe Cys Tyr Val Asn Asp Ile Val Leu Gly Ile 160 165 170 ctc gag ttg ctt aag atg ttt agg cgg gtt ctc tac att gat atc gat 578Leu Glu Leu Leu Lys Met Phe Arg Arg Val Leu Tyr Ile Asp Ile Asp 175 180 185 gtt cac cat gga gat gga gta gag gaa gcg ttt tac acc act gat aga 626Val His His Gly Asp Gly Val Glu Glu Ala Phe Tyr Thr Thr Asp Arg 190 195 200 gtt atg acc gtt tct ttt cac aag ttt ggg gac ttc ttc cct gga act 674Val Met Thr Val Ser Phe His Lys Phe Gly Asp Phe Phe Pro Gly Thr 205 210 215 ggt cac atc aga gac gtt ggc gct gag aaa ggg aag tac tat gct ctc 722Gly His Ile Arg Asp Val Gly Ala Glu Lys Gly Lys Tyr Tyr Ala Leu 220 225 230 235 aat gtc ccg ttg aac gat ggt atg gac gat gag agt ttc cgc agc ttg 770Asn Val Pro Leu Asn Asp Gly Met Asp Asp Glu Ser Phe Arg Ser Leu 240 245 250 ttt aga cct ctt atc cag aag gtt atg gag gtt tat cgg cca gaa gca 818Phe Arg Pro Leu Ile Gln Lys Val Met Glu Val Tyr Arg Pro Glu Ala 255 260 265 gtt gtt ctt cag tgc ggg gct gac tcc ttg agc ggt gat cgg ctg ggt 866Val Val Leu Gln Cys Gly Ala Asp Ser Leu Ser Gly Asp Arg Leu Gly 270 275 280 tgc ttc aac ttg tca gtc aag ggc cat gct gat tgc ctc cgg ttc ttg 914Cys Phe Asn Leu Ser Val Lys Gly His Ala Asp Cys Leu Arg Phe Leu 285 290 295 aga tct tat aat gtt cct ctc atg gtc ttg ggt ggt gga ggg tat act 962Arg Ser Tyr Asn Val Pro Leu Met Val Leu Gly Gly Gly Gly Tyr Thr 300 305 310 315 att cgg aat gtt gct cgt tgc tgg tgt tat gag act gca gtt gcg gtt 1010Ile Arg Asn Val Ala Arg Cys Trp Cys Tyr Glu Thr Ala Val Ala Val 320 325 330 gga gta gag ccg gac aac aag cta ccg tac aat gag tac ttt gag tat 1058Gly Val Glu Pro Asp Asn Lys Leu Pro Tyr Asn Glu Tyr Phe Glu Tyr 335 340 345 ttc ggt cca gac tat acg ctt cat gtc gag cca ggc cca atg gag aat 1106Phe Gly Pro Asp Tyr Thr Leu His Val Glu Pro Gly Pro Met Glu Asn 350 355 360 ttg aac aca cca aaa gat atg gag agg ata agg aac aca ttg cta gaa 1154Leu Asn Thr Pro Lys Asp Met Glu Arg Ile Arg Asn Thr Leu Leu Glu 365 370 375 caa ctt tct gga cta ata cac gca cct agt gtg ccg ttt cag cac aca 1202Gln Leu Ser Gly Leu Ile His Ala Pro Ser Val Pro Phe Gln His Thr 380 385 390 395 cct cca gtt aat cga gtc tta gat gag ccg gaa gaa gac ttg gag aag 1250Pro Pro Val Asn Arg Val Leu Asp Glu Pro Glu Glu Asp Leu Glu Lys 400 405 410 aga cca aag cct cga att tgg agt gga act gcg aat tat gaa tca gac 1298Arg Pro Lys Pro Arg Ile Trp Ser Gly Thr Ala Asn Tyr Glu Ser Asp 415 420 425 agt gac gat gat gag aaa cct ctt ggt ggt ttc tca ggt att aat ggc 1346Ser Asp Asp Asp Glu Lys Pro Leu Gly Gly Phe Ser Gly Ile Asn Gly 430 435 440 cca act atg gac agg gac tct aca ggg gaa gat gaa atg gaa gat gat 1394Pro Thr Met Asp Arg Asp Ser Thr Gly Glu Asp Glu Met Glu Asp Asp 445 450 455 agc gca gag ccg gag gtg gat cca cca tcg tct tgaaaccagc ttgatgtagt 1447Ser Ala Glu Pro Glu Val Asp Pro Pro Ser Ser 460 465 470 gtcaaaagtt aaggaattga ttcttggtga tgcttttctt cagtatgtga tttttttttt 1507gtttgcaaag aaaccttttt gttttggcct cagacgtatt taataggaat gtatttccat 1567taccattcga aaaaaaaaaa aaaaaaa 159440470PRTBrassica napus 40Met Glu Thr Asp Glu Ser Gly Val Ser Leu Ala Ser Gly Pro Asp Gly 1 5 10 15 Arg Lys Arg Arg Val Ser Tyr Phe Tyr Glu Pro Thr Ile Gly Asn Tyr 20 25 30 Tyr Tyr Gly Gln Gly His Pro Met Lys Pro His Arg Ile Arg Met Ala 35 40 45 His Ser Leu Ile Val His Tyr Asn Leu His Arg Arg Leu Glu Ile Ser 50 55 60 Arg Pro Tyr Leu Ala Asp Ala Ala Asp Ile Gly Arg Phe His Ser Pro 65 70 75 80 Glu Tyr Val Asp Phe Leu Arg Ser Val Ser Pro Glu Ser Val Gly Asp 85 90 95 Ser Ser Ala Arg Asn Leu Arg Arg Phe Asn Val Gly Glu Asp Cys Pro 100 105 110 Val Phe Asp Gly Leu Phe Glu Phe Cys Arg Ala Ser Ala Gly Gly Ser 115 120 125 Ile Gly Ala Ala Val Lys Leu Asn Arg Gln Asp Ala Asp Ile Ala Ile 130 135 140 Asn Trp Gly Gly Gly Leu His His Ala Lys Lys Ser Glu Ala Ser Gly 145 150 155 160 Phe Cys Tyr Val Asn Asp Ile Val Leu Gly Ile Leu Glu Leu Leu Lys 165 170 175 Met Phe Arg Arg Val Leu Tyr Ile Asp Ile Asp Val His His Gly Asp 180 185 190 Gly Val Glu Glu Ala Phe Tyr Thr Thr Asp Arg Val Met Thr Val Ser 195 200 205 Phe His Lys Phe Gly Asp Phe Phe Pro Gly Thr Gly His Ile Arg Asp 210 215 220 Val Gly Ala Glu Lys Gly Lys Tyr Tyr Ala Leu Asn Val Pro Leu Asn 225 230 235 240 Asp Gly Met Asp Asp Glu Ser Phe Arg Ser Leu Phe Arg Pro Leu Ile 245 250 255 Gln Lys Val Met Glu Val Tyr Arg Pro Glu Ala Val Val Leu Gln Cys 260 265 270 Gly Ala Asp Ser Leu Ser Gly Asp Arg Leu Gly Cys Phe Asn Leu Ser 275 280 285 Val Lys Gly His Ala Asp Cys Leu Arg Phe Leu Arg Ser Tyr Asn Val 290 295 300 Pro Leu Met Val Leu Gly Gly Gly Gly Tyr Thr Ile Arg Asn Val Ala 305 310 315 320 Arg Cys Trp Cys Tyr Glu Thr Ala Val Ala Val Gly Val Glu Pro Asp 325 330 335 Asn Lys Leu Pro Tyr Asn Glu Tyr Phe Glu Tyr Phe Gly Pro Asp Tyr 340 345 350 Thr Leu His Val Glu Pro Gly Pro Met Glu Asn Leu Asn Thr Pro Lys 355 360 365 Asp Met Glu Arg Ile Arg Asn Thr Leu Leu Glu Gln Leu Ser Gly Leu 370 375 380 Ile His Ala Pro Ser Val Pro Phe Gln His Thr Pro Pro Val Asn Arg 385 390 395 400 Val Leu Asp Glu Pro Glu Glu Asp Leu Glu Lys Arg Pro Lys Pro Arg 405 410 415 Ile Trp Ser Gly Thr Ala Asn Tyr Glu Ser Asp Ser Asp Asp Asp Glu 420 425 430 Lys Pro Leu Gly Gly Phe Ser Gly Ile Asn Gly Pro Thr Met Asp Arg 435 440 445 Asp Ser Thr Gly Glu Asp Glu Met Glu Asp Asp Ser Ala Glu Pro Glu 450 455 460 Val Asp Pro Pro Ser Ser 465 470 411367DNAZea maysCDS(12)..(1100) 41gatttcgtgg c atg ctg gcc cac gac gcc ggc cgc ggc gtg ttc gac tcg 50 Met Leu Ala His Asp Ala Gly Arg Gly Val Phe Asp Ser 1 5 10 ggc cgc gac ccc gga ttc ctg gat gtg ctc gac caa cac ccg gag aac 98Gly Arg Asp Pro Gly Phe Leu Asp Val Leu Asp Gln His Pro Glu Asn 15 20 25 gcc gac cgc gtc cgc aac atg gtc tcc atc ctc cgc cgc ggg ccc atc 146Ala Asp Arg Val Arg Asn Met Val Ser Ile Leu Arg Arg Gly Pro Ile 30 35 40 45 gcg cac ttc ctc tcc tgg cac tcg ggc cgc cct gcc cac gcc tcc gag 194Ala His Phe Leu Ser Trp His Ser Gly Arg Pro Ala His Ala Ser Glu 50 55 60 ctc ctc tcc ttc cac tcc tca gaa tac ata gag gag ctc gtc cag acg 242Leu Leu Ser Phe His Ser Ser Glu Tyr Ile Glu Glu Leu Val Gln Thr 65 70 75 aac gcc acc gga gcc aag aag aag ctc tgt gag ggc acg ttc ttg aac 290Asn Ala Thr Gly Ala Lys Lys Lys Leu Cys Glu Gly Thr Phe Leu Asn 80 85 90 ccg ggc tcc tgg ggt gcg gcg ctt cta gcg gcc ggg acc acg ctc tcc 338Pro Gly Ser Trp Gly Ala Ala Leu Leu Ala Ala Gly Thr Thr Leu Ser 95 100 105 tcc gcg aag cac ata cta gac ggg cag ggg aac ctg gcc tac gcg ttg 386Ser Ala Lys His Ile Leu Asp Gly Gln Gly Asn Leu Ala Tyr Ala Leu 110 115 120 125 gtt cgc ccc cct ggc cac cac gcg cag ccc gac cac gcc gat ggc tac 434Val Arg Pro Pro Gly His His Ala Gln Pro Asp His Ala Asp Gly Tyr 130 135 140 tgc ttt ctg aac aat gcc gga ctt gct gtg caa ctg gct ctg gat tcc 482Cys Phe Leu Asn Asn Ala Gly Leu Ala Val Gln Leu Ala Leu Asp Ser 145 150 155 ggg cgc gca aag gtc gcc gtt gtg gat att gat gtg cac tac ggg aat 530Gly Arg Ala Lys Val Ala Val Val Asp Ile Asp Val His Tyr Gly Asn 160 165 170 ggc acc gcg gag ggc ttc tat cgg aca gac acc gtg ttg acg atg tct 578Gly Thr Ala Glu Gly Phe Tyr Arg Thr Asp Thr Val Leu Thr Met Ser 175 180 185 ctt cac atg atg cat ggt tct tgg ggg cca tcg cat ccg cag agt ggc 626Leu His Met Met His Gly Ser Trp Gly Pro Ser His Pro Gln Ser Gly 190 195 200 205 tcc gtc gat gag att ggt gag ggc aag ggg ctt ggg tac aat ctc aat 674Ser Val Asp Glu Ile Gly Glu Gly Lys Gly Leu Gly Tyr Asn Leu Asn 210 215 220 ata cct ttg cct aat gga agt gga gat gct ggg tat gaa tat gcg atg 722Ile Pro Leu Pro Asn Gly Ser Gly Asp Ala Gly Tyr Glu Tyr Ala Met 225 230 235 aac gag ttg gtt gtt cca tcg att gat aag ttt cag cct caa ctg ttg 770Asn Glu Leu Val Val Pro Ser Ile Asp Lys Phe Gln Pro Gln Leu Leu 240 245 250 ttt ctt gtg gtc ggc caa gat tcc agt gcg ttt gat ccc aat gga aga 818Phe Leu Val Val Gly Gln Asp Ser Ser Ala Phe Asp Pro Asn Gly Arg 255 260 265 cag tgc ttg acc atg gaa ggc tac agg aaa att gga caa ata atg agg 866Gln Cys Leu Thr Met Glu Gly Tyr Arg Lys Ile Gly Gln Ile Met Arg 270 275 280 285 cgc ctg gct gat cgc cat tgc aat ggg caa ata ctg gtt gtc cag gaa 914Arg Leu Ala Asp Arg His Cys Asn Gly Gln Ile Leu Val Val Gln Glu 290 295 300 ggg ggt tac cac atc act tat tcg gca tat tgt ctg cat gct aca ctg 962Gly Gly Tyr His Ile Thr Tyr Ser Ala Tyr Cys Leu His Ala Thr Leu 305 310 315 gaa gga gtt ttg gat ctg gaa gcc ccg ctg ctt gat gac cca atc gct 1010Glu Gly Val Leu Asp Leu Glu Ala Pro Leu Leu Asp Asp Pro Ile Ala 320 325 330 tat tat ccg gag gat gat aaa tac act atg aaa gtc gtt gac atg ata 1058Tyr Tyr Pro Glu Asp Asp Lys Tyr Thr Met Lys Val Val Asp Met Ile 335 340 345 aag agc tac tgg aag gaa tcg gtt cct ttc cta aag gaa att 1100Lys Ser Tyr Trp Lys Glu Ser Val Pro Phe Leu Lys Glu Ile 350 355 360 tagaggaaat aatgttcgcg ccctcaatgt tcaaattgca ggaaactcca cattcctgga 1160catcttcaat tgaattggga agaacattgc acgtctacgt tttcaggccg tttaagatta 1220tctgattgac agtttcacgc ctaacattgt acaggataga ttaagaacat tgtttgctgc 1280tgctcttgaa tttctgcaat caggatctcg tcagcctatt tttgataaac agtcgatctt 1340ttttttttca aaaaaaaaaa aaaaaaa 136742363PRTZea mays 42Met Leu Ala His Asp Ala Gly Arg Gly Val Phe Asp Ser Gly Arg Asp 1 5 10 15 Pro Gly Phe Leu Asp Val Leu Asp Gln His Pro Glu Asn Ala Asp Arg 20 25 30 Val Arg Asn Met Val Ser Ile Leu Arg Arg Gly Pro Ile Ala His Phe 35 40 45 Leu Ser Trp His Ser Gly Arg Pro Ala His Ala Ser Glu Leu Leu Ser 50 55

60 Phe His Ser Ser Glu Tyr Ile Glu Glu Leu Val Gln Thr Asn Ala Thr 65 70 75 80 Gly Ala Lys Lys Lys Leu Cys Glu Gly Thr Phe Leu Asn Pro Gly Ser 85 90 95 Trp Gly Ala Ala Leu Leu Ala Ala Gly Thr Thr Leu Ser Ser Ala Lys 100 105 110 His Ile Leu Asp Gly Gln Gly Asn Leu Ala Tyr Ala Leu Val Arg Pro 115 120 125 Pro Gly His His Ala Gln Pro Asp His Ala Asp Gly Tyr Cys Phe Leu 130 135 140 Asn Asn Ala Gly Leu Ala Val Gln Leu Ala Leu Asp Ser Gly Arg Ala 145 150 155 160 Lys Val Ala Val Val Asp Ile Asp Val His Tyr Gly Asn Gly Thr Ala 165 170 175 Glu Gly Phe Tyr Arg Thr Asp Thr Val Leu Thr Met Ser Leu His Met 180 185 190 Met His Gly Ser Trp Gly Pro Ser His Pro Gln Ser Gly Ser Val Asp 195 200 205 Glu Ile Gly Glu Gly Lys Gly Leu Gly Tyr Asn Leu Asn Ile Pro Leu 210 215 220 Pro Asn Gly Ser Gly Asp Ala Gly Tyr Glu Tyr Ala Met Asn Glu Leu 225 230 235 240 Val Val Pro Ser Ile Asp Lys Phe Gln Pro Gln Leu Leu Phe Leu Val 245 250 255 Val Gly Gln Asp Ser Ser Ala Phe Asp Pro Asn Gly Arg Gln Cys Leu 260 265 270 Thr Met Glu Gly Tyr Arg Lys Ile Gly Gln Ile Met Arg Arg Leu Ala 275 280 285 Asp Arg His Cys Asn Gly Gln Ile Leu Val Val Gln Glu Gly Gly Tyr 290 295 300 His Ile Thr Tyr Ser Ala Tyr Cys Leu His Ala Thr Leu Glu Gly Val 305 310 315 320 Leu Asp Leu Glu Ala Pro Leu Leu Asp Asp Pro Ile Ala Tyr Tyr Pro 325 330 335 Glu Asp Asp Lys Tyr Thr Met Lys Val Val Asp Met Ile Lys Ser Tyr 340 345 350 Trp Lys Glu Ser Val Pro Phe Leu Lys Glu Ile 355 360 431598DNALinum usitatissimumCDS(101)..(1387) 43gctctgacaa tggcggagtc gtctttgtaa gctcagctca actttcttcg ccctctctgc 60tcaaccctcg tccttctcgt cgctgaattc ggtctgagaa atg aag tcg aag gac 115 Met Lys Ser Lys Asp 1 5 aaa atc tcc tac ttt tac gat ggg gat gtt ggc agt gtt tac ttc ggt 163Lys Ile Ser Tyr Phe Tyr Asp Gly Asp Val Gly Ser Val Tyr Phe Gly 10 15 20 ccg aat cac ccg atg aaa cca cac agg ctc tgt atg acc cac cat ctt 211Pro Asn His Pro Met Lys Pro His Arg Leu Cys Met Thr His His Leu 25 30 35 gtt ctt tct tat gac ctt cac aag aag atg gag att tat cgg cca cac 259Val Leu Ser Tyr Asp Leu His Lys Lys Met Glu Ile Tyr Arg Pro His 40 45 50 aag gca tac cct gtt gag cta gct cag ttc cat tct gct gat tac gtt 307Lys Ala Tyr Pro Val Glu Leu Ala Gln Phe His Ser Ala Asp Tyr Val 55 60 65 gag ttc ttg cac cgg att aca cct gat act cag cac ttg tac aga act 355Glu Phe Leu His Arg Ile Thr Pro Asp Thr Gln His Leu Tyr Arg Thr 70 75 80 85 gat tta gca aga tat aat ctt gga gaa gat tgc ccc gtg ttc gag aat 403Asp Leu Ala Arg Tyr Asn Leu Gly Glu Asp Cys Pro Val Phe Glu Asn 90 95 100 ctg ttt gaa ttt tgt caa atc tat gct ggg ggg aca ata gac gcc gct 451Leu Phe Glu Phe Cys Gln Ile Tyr Ala Gly Gly Thr Ile Asp Ala Ala 105 110 115 cga aga ttg aac aat caa ctg tgt gat att gct ata aat tgg gct ggt 499Arg Arg Leu Asn Asn Gln Leu Cys Asp Ile Ala Ile Asn Trp Ala Gly 120 125 130 gga tta cat cat gcc aaa aag tgc gag gct tct gga ttt tgt tac atc 547Gly Leu His His Ala Lys Lys Cys Glu Ala Ser Gly Phe Cys Tyr Ile 135 140 145 aac gac ttg gtt ctc ggg atc ttg gag ctt cta aaa tac cat gct cgt 595Asn Asp Leu Val Leu Gly Ile Leu Glu Leu Leu Lys Tyr His Ala Arg 150 155 160 165 gtt ctg tac ata gac ata gat gtc cat cat ggt gat ggc gta gag gag 643Val Leu Tyr Ile Asp Ile Asp Val His His Gly Asp Gly Val Glu Glu 170 175 180 gcc ttc tat ttt act gac agg gtg atg act gta agt ttt cac aag ttt 691Ala Phe Tyr Phe Thr Asp Arg Val Met Thr Val Ser Phe His Lys Phe 185 190 195 gga gat ttg ttc ttc ccg gga acc ggt gat gtt aag gaa ata gga gaa 739Gly Asp Leu Phe Phe Pro Gly Thr Gly Asp Val Lys Glu Ile Gly Glu 200 205 210 aga gaa ggg aag ttc tac gcc ata aat gtt cct ctt agg gac ggg ata 787Arg Glu Gly Lys Phe Tyr Ala Ile Asn Val Pro Leu Arg Asp Gly Ile 215 220 225 gat gac tcg agc ttc aac cgt ctc ttt aaa acc atc ata tct aag gtt 835Asp Asp Ser Ser Phe Asn Arg Leu Phe Lys Thr Ile Ile Ser Lys Val 230 235 240 245 gta gaa atc tac caa cct ggc gct ata gtt ctc caa tgc gga gca gat 883Val Glu Ile Tyr Gln Pro Gly Ala Ile Val Leu Gln Cys Gly Ala Asp 250 255 260 tcg ctg gct ggg gac cgt ttg ggc tgt ttc aat ctc tcg att gat gga 931Ser Leu Ala Gly Asp Arg Leu Gly Cys Phe Asn Leu Ser Ile Asp Gly 265 270 275 cat gcg gaa tgt gtt aag ttt gtg aag aag ttc aac att cct tta ctg 979His Ala Glu Cys Val Lys Phe Val Lys Lys Phe Asn Ile Pro Leu Leu 280 285 290 gtt act gga ggt gga gga tat acg aag gag aat gta gct cga tgc tgg 1027Val Thr Gly Gly Gly Gly Tyr Thr Lys Glu Asn Val Ala Arg Cys Trp 295 300 305 aca gtt gaa aca gga gta ctg ctg gat act gaa ctg ccc aat gag atc 1075Thr Val Glu Thr Gly Val Leu Leu Asp Thr Glu Leu Pro Asn Glu Ile 310 315 320 325 ccc gaa aac gag tac ata aag tat ttc gga cct gac tat act ttg aag 1123Pro Glu Asn Glu Tyr Ile Lys Tyr Phe Gly Pro Asp Tyr Thr Leu Lys 330 335 340 att ccg agc aga tac att gag aat ttg aac agt aaa tct tat ctc agc 1171Ile Pro Ser Arg Tyr Ile Glu Asn Leu Asn Ser Lys Ser Tyr Leu Ser 345 350 355 tcc ctc aaa gtt caa gta atg gag aat ttg cgg tac att cag cac gct 1219Ser Leu Lys Val Gln Val Met Glu Asn Leu Arg Tyr Ile Gln His Ala 360 365 370 cca agt gtg cag atg caa gag gtt cca ccg gat ttt tac atc cca gac 1267Pro Ser Val Gln Met Gln Glu Val Pro Pro Asp Phe Tyr Ile Pro Asp 375 380 385 ttc gac gaa gat gag cag aac cca gat gaa cga atg gat cag cat act 1315Phe Asp Glu Asp Glu Gln Asn Pro Asp Glu Arg Met Asp Gln His Thr 390 395 400 405 cga gac aag caa gtc cag cgg gac gat gaa tac tac gat ggg gac aat 1363Arg Asp Lys Gln Val Gln Arg Asp Asp Glu Tyr Tyr Asp Gly Asp Asn 410 415 420 gac aac gac ccc aca gat cga tca tgatggtgcg gtagtggagc tttagtgttc 1417Asp Asn Asp Pro Thr Asp Arg Ser 425 atgctagttc agatgttttg tacatgctat actgatataa gcttgactct gtgatatcag 1477gatttcaatt ggtcaaaatc ccttgtgtaa accattccat tccataggga tatatcgctt 1537ttgtaactct taaaagcaca atgctttagt tggaaatggg aactcgggag accgacacgc 1597a 159844429PRTLinum usitatissimum 44Met Lys Ser Lys Asp Lys Ile Ser Tyr Phe Tyr Asp Gly Asp Val Gly 1 5 10 15 Ser Val Tyr Phe Gly Pro Asn His Pro Met Lys Pro His Arg Leu Cys 20 25 30 Met Thr His His Leu Val Leu Ser Tyr Asp Leu His Lys Lys Met Glu 35 40 45 Ile Tyr Arg Pro His Lys Ala Tyr Pro Val Glu Leu Ala Gln Phe His 50 55 60 Ser Ala Asp Tyr Val Glu Phe Leu His Arg Ile Thr Pro Asp Thr Gln 65 70 75 80 His Leu Tyr Arg Thr Asp Leu Ala Arg Tyr Asn Leu Gly Glu Asp Cys 85 90 95 Pro Val Phe Glu Asn Leu Phe Glu Phe Cys Gln Ile Tyr Ala Gly Gly 100 105 110 Thr Ile Asp Ala Ala Arg Arg Leu Asn Asn Gln Leu Cys Asp Ile Ala 115 120 125 Ile Asn Trp Ala Gly Gly Leu His His Ala Lys Lys Cys Glu Ala Ser 130 135 140 Gly Phe Cys Tyr Ile Asn Asp Leu Val Leu Gly Ile Leu Glu Leu Leu 145 150 155 160 Lys Tyr His Ala Arg Val Leu Tyr Ile Asp Ile Asp Val His His Gly 165 170 175 Asp Gly Val Glu Glu Ala Phe Tyr Phe Thr Asp Arg Val Met Thr Val 180 185 190 Ser Phe His Lys Phe Gly Asp Leu Phe Phe Pro Gly Thr Gly Asp Val 195 200 205 Lys Glu Ile Gly Glu Arg Glu Gly Lys Phe Tyr Ala Ile Asn Val Pro 210 215 220 Leu Arg Asp Gly Ile Asp Asp Ser Ser Phe Asn Arg Leu Phe Lys Thr 225 230 235 240 Ile Ile Ser Lys Val Val Glu Ile Tyr Gln Pro Gly Ala Ile Val Leu 245 250 255 Gln Cys Gly Ala Asp Ser Leu Ala Gly Asp Arg Leu Gly Cys Phe Asn 260 265 270 Leu Ser Ile Asp Gly His Ala Glu Cys Val Lys Phe Val Lys Lys Phe 275 280 285 Asn Ile Pro Leu Leu Val Thr Gly Gly Gly Gly Tyr Thr Lys Glu Asn 290 295 300 Val Ala Arg Cys Trp Thr Val Glu Thr Gly Val Leu Leu Asp Thr Glu 305 310 315 320 Leu Pro Asn Glu Ile Pro Glu Asn Glu Tyr Ile Lys Tyr Phe Gly Pro 325 330 335 Asp Tyr Thr Leu Lys Ile Pro Ser Arg Tyr Ile Glu Asn Leu Asn Ser 340 345 350 Lys Ser Tyr Leu Ser Ser Leu Lys Val Gln Val Met Glu Asn Leu Arg 355 360 365 Tyr Ile Gln His Ala Pro Ser Val Gln Met Gln Glu Val Pro Pro Asp 370 375 380 Phe Tyr Ile Pro Asp Phe Asp Glu Asp Glu Gln Asn Pro Asp Glu Arg 385 390 395 400 Met Asp Gln His Thr Arg Asp Lys Gln Val Gln Arg Asp Asp Glu Tyr 405 410 415 Tyr Asp Gly Asp Asn Asp Asn Asp Pro Thr Asp Arg Ser 420 425 451854DNAOryza sativaCDS(64)..(1617) 45aaagaaaaaa aaaagagata aaaaaaaaat ctcctcctcc tcgccgccgc cgccgccgcc 60gcc atg gac gcc tcc gcc gga ggc ggg ggg aac tcg ctg ccg acg gcg 108 Met Asp Ala Ser Ala Gly Gly Gly Gly Asn Ser Leu Pro Thr Ala 1 5 10 15 ggg gcc gac ggg gcc aag cgg cgg gtg tgc tac ttc tac gac gcg gag 156Gly Ala Asp Gly Ala Lys Arg Arg Val Cys Tyr Phe Tyr Asp Ala Glu 20 25 30 gtg ggg aac tac tac tac ggg cag ggg cac ccg atg aag ccg cac cgc 204Val Gly Asn Tyr Tyr Tyr Gly Gln Gly His Pro Met Lys Pro His Arg 35 40 45 atc cgg atg acc cac gcg ctg ctc gcc cac tac ggc ctc ctc gac cag 252Ile Arg Met Thr His Ala Leu Leu Ala His Tyr Gly Leu Leu Asp Gln 50 55 60 atg cag gtg ctc aag ccc cac ccg gcg cgc gac cgc gac ctc tgc cgc 300Met Gln Val Leu Lys Pro His Pro Ala Arg Asp Arg Asp Leu Cys Arg 65 70 75 ttc cac gcc gac gac tac gtc gcc ttc ctc cgc tcc gtc acg ccg gag 348Phe His Ala Asp Asp Tyr Val Ala Phe Leu Arg Ser Val Thr Pro Glu 80 85 90 95 acc cag cag gac cag atc cgg gcg ctc aag cgc ttc aac gtc ggc gag 396Thr Gln Gln Asp Gln Ile Arg Ala Leu Lys Arg Phe Asn Val Gly Glu 100 105 110 gac tgc ccc gtc ttc gac ggc ctc tac agc ttc tgc cag acc tac gcc 444Asp Cys Pro Val Phe Asp Gly Leu Tyr Ser Phe Cys Gln Thr Tyr Ala 115 120 125 ggg gga tcc gtc ggc ggc gcc gtc aag ctc aac cac ggc cac gac atc 492Gly Gly Ser Val Gly Gly Ala Val Lys Leu Asn His Gly His Asp Ile 130 135 140 gcc atc aac tgg gcc ggc ggc ctc cac cac gcc aag aag tgc gag gcc 540Ala Ile Asn Trp Ala Gly Gly Leu His His Ala Lys Lys Cys Glu Ala 145 150 155 tcg gga ttc tgc tac gtc aac gac atc gtc ctc gcc atc ctc gag ctc 588Ser Gly Phe Cys Tyr Val Asn Asp Ile Val Leu Ala Ile Leu Glu Leu 160 165 170 175 ctc aaa tac cac cag cgt gtt ctc tat gtg gat atc gat atc cac cat 636Leu Lys Tyr His Gln Arg Val Leu Tyr Val Asp Ile Asp Ile His His 180 185 190 ggg gat ggt gtg gag gag gcg ttc tac acg acg gac agg gtg atg acg 684Gly Asp Gly Val Glu Glu Ala Phe Tyr Thr Thr Asp Arg Val Met Thr 195 200 205 gtc tcg ttc cac aag ttt ggg gat tat ttc ccg ggg acc ggg gac att 732Val Ser Phe His Lys Phe Gly Asp Tyr Phe Pro Gly Thr Gly Asp Ile 210 215 220 cgc gat att ggg cac tca aag ggg aag tat tac tct ctg aat gtc ccg 780Arg Asp Ile Gly His Ser Lys Gly Lys Tyr Tyr Ser Leu Asn Val Pro 225 230 235 ttg gac gac ggt atc gac gac gag agc tac cag tcg ttg ttc aag ccg 828Leu Asp Asp Gly Ile Asp Asp Glu Ser Tyr Gln Ser Leu Phe Lys Pro 240 245 250 255 atc atg ggg aag gtg atg gag gtt ttt cgc cct ggc gcg gtg gtg ctc 876Ile Met Gly Lys Val Met Glu Val Phe Arg Pro Gly Ala Val Val Leu 260 265 270 cag tgc ggt gcg gac tct ctg tcg ggt gat agg ttg ggt tgc ttc aac 924Gln Cys Gly Ala Asp Ser Leu Ser Gly Asp Arg Leu Gly Cys Phe Asn 275 280 285 ctg tca atc agg ggc cac gcg gaa tgc gtg aga ttc atg agg tcc ttc 972Leu Ser Ile Arg Gly His Ala Glu Cys Val Arg Phe Met Arg Ser Phe 290 295 300 aat gtc ccg ctg ttg ctg ctt ggt ggt ggt ggg tat acc ata aga aat 1020Asn Val Pro Leu Leu Leu Leu Gly Gly Gly Gly Tyr Thr Ile Arg Asn 305 310 315 gtt gcg cgg tgt tgg tgc tat gag aca gga gtt gca ctt ggt cat gag 1068Val Ala Arg Cys Trp Cys Tyr Glu Thr Gly Val Ala Leu Gly His Glu 320 325 330 335 ctc act gac aag atg cct cca aat gag tat ttt gag tac ttt ggt cca 1116Leu Thr Asp Lys Met Pro Pro Asn Glu Tyr Phe Glu Tyr Phe Gly Pro 340 345 350 gat tat aca ctt cat gtt gca cca agt aac atg gag aac aaa aac aca 1164Asp Tyr Thr Leu His Val Ala Pro Ser Asn Met Glu Asn Lys Asn Thr 355 360 365 cgc cag cag ttg gat gat ata aga tca aga ctt ctt gat aat ctt tca 1212Arg Gln Gln Leu Asp Asp Ile Arg Ser Arg Leu Leu Asp Asn Leu Ser 370 375 380 aaa ctt cga cat gct cct agc gtc caa ttt caa gag cga ccc cct gag 1260Lys Leu Arg His Ala Pro Ser Val Gln Phe Gln Glu Arg Pro Pro Glu 385 390 395 gct gag cta cct gag caa gat gaa gac caa gag gat cct gat gaa agg 1308Ala Glu Leu Pro Glu Gln Asp Glu Asp Gln Glu Asp Pro Asp Glu Arg 400 405 410 415 cac cat gct gat tct gat gtg gaa atg gat gat gtc aaa cct ttg gat 1356His His Ala Asp Ser Asp Val Glu Met Asp Asp

Val Lys Pro Leu Asp 420 425 430 gac tca gga agg agg agc agt att cag aat gtg aga gtt aag aga gag 1404Asp Ser Gly Arg Arg Ser Ser Ile Gln Asn Val Arg Val Lys Arg Glu 435 440 445 tct gct gaa aca gat gcc gca gat cag gat ggt aat agg gtc gct gca 1452Ser Ala Glu Thr Asp Ala Ala Asp Gln Asp Gly Asn Arg Val Ala Ala 450 455 460 gag aac acc aag ggc aca gaa cct gcg gct gat gga gtt ggt tcc tcg 1500Glu Asn Thr Lys Gly Thr Glu Pro Ala Ala Asp Gly Val Gly Ser Ser 465 470 475 aaa caa act gtt cct acc gat gca agt gcg atg gcc ata gac gaa cca 1548Lys Gln Thr Val Pro Thr Asp Ala Ser Ala Met Ala Ile Asp Glu Pro 480 485 490 495 ggc tcc ctg aaa gtc gag cca gat aac tca aac aaa ttg caa gat caa 1596Gly Ser Leu Lys Val Glu Pro Asp Asn Ser Asn Lys Leu Gln Asp Gln 500 505 510 cca tcg gtg cac cag aag aca taatagttct ctctacctta aaacttagta 1647Pro Ser Val His Gln Lys Thr 515 actgatgcca tctatcatcc attgattata ttggagaaac tcccaacttt gaagcagaga 1707gttcatgcca taccaaaagt tatataccaa atttcgaatg gtatgtacac ctttcgaact 1767ggtggtgttt tgtgcaatac atttatgcca ggctgactat tatgtggtat ctattattag 1827ctttagtata aaaaaaaaaa aaaaaaa 185446518PRTOryza sativa 46Met Asp Ala Ser Ala Gly Gly Gly Gly Asn Ser Leu Pro Thr Ala Gly 1 5 10 15 Ala Asp Gly Ala Lys Arg Arg Val Cys Tyr Phe Tyr Asp Ala Glu Val 20 25 30 Gly Asn Tyr Tyr Tyr Gly Gln Gly His Pro Met Lys Pro His Arg Ile 35 40 45 Arg Met Thr His Ala Leu Leu Ala His Tyr Gly Leu Leu Asp Gln Met 50 55 60 Gln Val Leu Lys Pro His Pro Ala Arg Asp Arg Asp Leu Cys Arg Phe 65 70 75 80 His Ala Asp Asp Tyr Val Ala Phe Leu Arg Ser Val Thr Pro Glu Thr 85 90 95 Gln Gln Asp Gln Ile Arg Ala Leu Lys Arg Phe Asn Val Gly Glu Asp 100 105 110 Cys Pro Val Phe Asp Gly Leu Tyr Ser Phe Cys Gln Thr Tyr Ala Gly 115 120 125 Gly Ser Val Gly Gly Ala Val Lys Leu Asn His Gly His Asp Ile Ala 130 135 140 Ile Asn Trp Ala Gly Gly Leu His His Ala Lys Lys Cys Glu Ala Ser 145 150 155 160 Gly Phe Cys Tyr Val Asn Asp Ile Val Leu Ala Ile Leu Glu Leu Leu 165 170 175 Lys Tyr His Gln Arg Val Leu Tyr Val Asp Ile Asp Ile His His Gly 180 185 190 Asp Gly Val Glu Glu Ala Phe Tyr Thr Thr Asp Arg Val Met Thr Val 195 200 205 Ser Phe His Lys Phe Gly Asp Tyr Phe Pro Gly Thr Gly Asp Ile Arg 210 215 220 Asp Ile Gly His Ser Lys Gly Lys Tyr Tyr Ser Leu Asn Val Pro Leu 225 230 235 240 Asp Asp Gly Ile Asp Asp Glu Ser Tyr Gln Ser Leu Phe Lys Pro Ile 245 250 255 Met Gly Lys Val Met Glu Val Phe Arg Pro Gly Ala Val Val Leu Gln 260 265 270 Cys Gly Ala Asp Ser Leu Ser Gly Asp Arg Leu Gly Cys Phe Asn Leu 275 280 285 Ser Ile Arg Gly His Ala Glu Cys Val Arg Phe Met Arg Ser Phe Asn 290 295 300 Val Pro Leu Leu Leu Leu Gly Gly Gly Gly Tyr Thr Ile Arg Asn Val 305 310 315 320 Ala Arg Cys Trp Cys Tyr Glu Thr Gly Val Ala Leu Gly His Glu Leu 325 330 335 Thr Asp Lys Met Pro Pro Asn Glu Tyr Phe Glu Tyr Phe Gly Pro Asp 340 345 350 Tyr Thr Leu His Val Ala Pro Ser Asn Met Glu Asn Lys Asn Thr Arg 355 360 365 Gln Gln Leu Asp Asp Ile Arg Ser Arg Leu Leu Asp Asn Leu Ser Lys 370 375 380 Leu Arg His Ala Pro Ser Val Gln Phe Gln Glu Arg Pro Pro Glu Ala 385 390 395 400 Glu Leu Pro Glu Gln Asp Glu Asp Gln Glu Asp Pro Asp Glu Arg His 405 410 415 His Ala Asp Ser Asp Val Glu Met Asp Asp Val Lys Pro Leu Asp Asp 420 425 430 Ser Gly Arg Arg Ser Ser Ile Gln Asn Val Arg Val Lys Arg Glu Ser 435 440 445 Ala Glu Thr Asp Ala Ala Asp Gln Asp Gly Asn Arg Val Ala Ala Glu 450 455 460 Asn Thr Lys Gly Thr Glu Pro Ala Ala Asp Gly Val Gly Ser Ser Lys 465 470 475 480 Gln Thr Val Pro Thr Asp Ala Ser Ala Met Ala Ile Asp Glu Pro Gly 485 490 495 Ser Leu Lys Val Glu Pro Asp Asn Ser Asn Lys Leu Gln Asp Gln Pro 500 505 510 Ser Val His Gln Lys Thr 515 471252DNAGlycine maxCDS(4)..(1005) 47ggg atg ctg aag cat gac acg ggc aat ggc gtg ttt gac acg ggg atg 48 Met Leu Lys His Asp Thr Gly Asn Gly Val Phe Asp Thr Gly Met 1 5 10 15 gat cca ggc ttc tta gag gtg ttg gag aag cac cct gaa aac tca gac 96Asp Pro Gly Phe Leu Glu Val Leu Glu Lys His Pro Glu Asn Ser Asp 20 25 30 aga gtg aaa aac cta gtg tct att ctc aaa agg ggt cct atc tcc cct 144Arg Val Lys Asn Leu Val Ser Ile Leu Lys Arg Gly Pro Ile Ser Pro 35 40 45 tac att tct tgg cac ctt ggt aca cct gca aaa atc cct gag ctt ttt 192Tyr Ile Ser Trp His Leu Gly Thr Pro Ala Lys Ile Pro Glu Leu Phe 50 55 60 tct ttt cac act cct gaa tac ata aat gaa ctg gta gaa gtt gat aaa 240Ser Phe His Thr Pro Glu Tyr Ile Asn Glu Leu Val Glu Val Asp Lys 65 70 75 gaa ggg ggg aag cag ctt tgt ggt ggg aca ttt ttg aac cct gga tca 288Glu Gly Gly Lys Gln Leu Cys Gly Gly Thr Phe Leu Asn Pro Gly Ser 80 85 90 95 tgg gat gct gca ctt ctt gct gct ggg act aca cta tct gcg atg aag 336Trp Asp Ala Ala Leu Leu Ala Ala Gly Thr Thr Leu Ser Ala Met Lys 100 105 110 cat tta ctg aat ggg gat gga aaa gtt tcc tat gca ttg gtt agg ccc 384His Leu Leu Asn Gly Asp Gly Lys Val Ser Tyr Ala Leu Val Arg Pro 115 120 125 cct ggt cac cat gct cag cct tct ctg gcc gat ggc tac tgt ttc ctt 432Pro Gly His His Ala Gln Pro Ser Leu Ala Asp Gly Tyr Cys Phe Leu 130 135 140 aac aat gca ggt cta gct gtg caa ttg gct tta gat tcc ggc tgc aag 480Asn Asn Ala Gly Leu Ala Val Gln Leu Ala Leu Asp Ser Gly Cys Lys 145 150 155 aag gtt gcg gtc ata gat att gat gtg cat tat gga aat gga acg gca 528Lys Val Ala Val Ile Asp Ile Asp Val His Tyr Gly Asn Gly Thr Ala 160 165 170 175 gag ggg ttt tat cga tct aat aag gtt ctt acc atc tct ctt cat atg 576Glu Gly Phe Tyr Arg Ser Asn Lys Val Leu Thr Ile Ser Leu His Met 180 185 190 aac cat gga tca tgg ggt cca tct cat ccg caa agt ggc tct gtt gat 624Asn His Gly Ser Trp Gly Pro Ser His Pro Gln Ser Gly Ser Val Asp 195 200 205 gag cta ggt gaa gga gaa ggt tat ggc ttt aac ttg aac ata cct cta 672Glu Leu Gly Glu Gly Glu Gly Tyr Gly Phe Asn Leu Asn Ile Pro Leu 210 215 220 cca aat gga act ggg gac aag gga tat gta cat gcc ttc aat gag ttg 720Pro Asn Gly Thr Gly Asp Lys Gly Tyr Val His Ala Phe Asn Glu Leu 225 230 235 gtt gtt cca tcc atc caa aag ttt ggg cct gat atg ata gtt ttg gtt 768Val Val Pro Ser Ile Gln Lys Phe Gly Pro Asp Met Ile Val Leu Val 240 245 250 255 ctt gga caa gac tct aat gca ttt gat ccc aat gga agg caa tgc tta 816Leu Gly Gln Asp Ser Asn Ala Phe Asp Pro Asn Gly Arg Gln Cys Leu 260 265 270 aca atg gag ggc tat aga gaa ata ggg cga att gtc cat ctt ctt gcg 864Thr Met Glu Gly Tyr Arg Glu Ile Gly Arg Ile Val His Leu Leu Ala 275 280 285 aaa agg cac agt gca gga cgc ctt cta att gtc cag gaa ggt gga tat 912Lys Arg His Ser Ala Gly Arg Leu Leu Ile Val Gln Glu Gly Gly Tyr 290 295 300 cat gtc aca tat tct gca tat tgt tta cat gca aca ctt gag ggg att 960His Val Thr Tyr Ser Ala Tyr Cys Leu His Ala Thr Leu Glu Gly Ile 305 310 315 ctc aac cta cca atg cct cta cta gcg gat cct att gct ttt acc 1005Leu Asn Leu Pro Met Pro Leu Leu Ala Asp Pro Ile Ala Phe Thr 320 325 330 tagacgacga gacattttct gtccaagtta tagaagccat taagaattat caaaaagata 1065aagtgtgcta gtggagaaac tcactagaca cttccttctg tgcgtgtgaa ataaatcttg 1125atacttttca agaagggtta atatttatcg tgtaactatt gagggatttt caaggttatt 1185ttaaataaat ttaatcttgt ccagttgatc ttttgattaa aaaaaaaaaa aaagagaacc 1245gaacgca 125248334PRTGlycine max 48Met Leu Lys His Asp Thr Gly Asn Gly Val Phe Asp Thr Gly Met Asp 1 5 10 15 Pro Gly Phe Leu Glu Val Leu Glu Lys His Pro Glu Asn Ser Asp Arg 20 25 30 Val Lys Asn Leu Val Ser Ile Leu Lys Arg Gly Pro Ile Ser Pro Tyr 35 40 45 Ile Ser Trp His Leu Gly Thr Pro Ala Lys Ile Pro Glu Leu Phe Ser 50 55 60 Phe His Thr Pro Glu Tyr Ile Asn Glu Leu Val Glu Val Asp Lys Glu 65 70 75 80 Gly Gly Lys Gln Leu Cys Gly Gly Thr Phe Leu Asn Pro Gly Ser Trp 85 90 95 Asp Ala Ala Leu Leu Ala Ala Gly Thr Thr Leu Ser Ala Met Lys His 100 105 110 Leu Leu Asn Gly Asp Gly Lys Val Ser Tyr Ala Leu Val Arg Pro Pro 115 120 125 Gly His His Ala Gln Pro Ser Leu Ala Asp Gly Tyr Cys Phe Leu Asn 130 135 140 Asn Ala Gly Leu Ala Val Gln Leu Ala Leu Asp Ser Gly Cys Lys Lys 145 150 155 160 Val Ala Val Ile Asp Ile Asp Val His Tyr Gly Asn Gly Thr Ala Glu 165 170 175 Gly Phe Tyr Arg Ser Asn Lys Val Leu Thr Ile Ser Leu His Met Asn 180 185 190 His Gly Ser Trp Gly Pro Ser His Pro Gln Ser Gly Ser Val Asp Glu 195 200 205 Leu Gly Glu Gly Glu Gly Tyr Gly Phe Asn Leu Asn Ile Pro Leu Pro 210 215 220 Asn Gly Thr Gly Asp Lys Gly Tyr Val His Ala Phe Asn Glu Leu Val 225 230 235 240 Val Pro Ser Ile Gln Lys Phe Gly Pro Asp Met Ile Val Leu Val Leu 245 250 255 Gly Gln Asp Ser Asn Ala Phe Asp Pro Asn Gly Arg Gln Cys Leu Thr 260 265 270 Met Glu Gly Tyr Arg Glu Ile Gly Arg Ile Val His Leu Leu Ala Lys 275 280 285 Arg His Ser Ala Gly Arg Leu Leu Ile Val Gln Glu Gly Gly Tyr His 290 295 300 Val Thr Tyr Ser Ala Tyr Cys Leu His Ala Thr Leu Glu Gly Ile Leu 305 310 315 320 Asn Leu Pro Met Pro Leu Leu Ala Asp Pro Ile Ala Phe Thr 325 330 491723DNAGlycine maxCDS(143)..(1429) 49acatagagtg attaggctgg tagcaagcgc gacatggcac tgcagtgcag gttaagctca 60caccattgat atcactagcc atattctcct ttcgctaatt cccgcaagct actactcttc 120ttcaacgctc tctctgcaaa ag atg cgc tcc aag gac aga atc gct tac ttc 172 Met Arg Ser Lys Asp Arg Ile Ala Tyr Phe 1 5 10 tac gac ggt gat gtc ggt agt gtt tac ttt ggg gcg aag cat ccg atg 220Tyr Asp Gly Asp Val Gly Ser Val Tyr Phe Gly Ala Lys His Pro Met 15 20 25 aag ccc cac cgg ctt tgc atg act cat cat ctt gtt ctc tca tac gat 268Lys Pro His Arg Leu Cys Met Thr His His Leu Val Leu Ser Tyr Asp 30 35 40 ctt cat aag aag atg gag att tat cgt cca cac aag gct tat cct gtt 316Leu His Lys Lys Met Glu Ile Tyr Arg Pro His Lys Ala Tyr Pro Val 45 50 55 gag ctt gcc cag ttt cat tca gct gat tat gtt gag ttt ttg aac agg 364Glu Leu Ala Gln Phe His Ser Ala Asp Tyr Val Glu Phe Leu Asn Arg 60 65 70 att aca cct gac act cag cac ttg ttc ttg aat gaa ctg aca aaa tat 412Ile Thr Pro Asp Thr Gln His Leu Phe Leu Asn Glu Leu Thr Lys Tyr 75 80 85 90 aat ctt gga gaa gac tgc cct gta ttt gac aac tta ttt gaa ttt tgt 460Asn Leu Gly Glu Asp Cys Pro Val Phe Asp Asn Leu Phe Glu Phe Cys 95 100 105 cag att tat gct ggt gga act ata gat gct gca cgt cga tta aac aat 508Gln Ile Tyr Ala Gly Gly Thr Ile Asp Ala Ala Arg Arg Leu Asn Asn 110 115 120 caa ctg tgt gat att gct atc aac tgg gcc ggt gga cta cat cat gcc 556Gln Leu Cys Asp Ile Ala Ile Asn Trp Ala Gly Gly Leu His His Ala 125 130 135 aag aaa tgc gag gca tct gga ttt tgt tac atc aat gac ttg gtt tta 604Lys Lys Cys Glu Ala Ser Gly Phe Cys Tyr Ile Asn Asp Leu Val Leu 140 145 150 gga atc ttg gag ctt ctt aaa tat cat gct cgt gtt ttg tat att gat 652Gly Ile Leu Glu Leu Leu Lys Tyr His Ala Arg Val Leu Tyr Ile Asp 155 160 165 170 ata gat gtg cac cat ggt gat ggt gta gaa gaa gcc ttc tac ttc act 700Ile Asp Val His His Gly Asp Gly Val Glu Glu Ala Phe Tyr Phe Thr 175 180 185 gac agg gtg atg act gtc agt ttt cac aag tat gga gat tcg ttc ttc 748Asp Arg Val Met Thr Val Ser Phe His Lys Tyr Gly Asp Ser Phe Phe 190 195 200 ccg ggt act ggc gat gct aag gaa ata gga gaa aga gaa gga aag ttt 796Pro Gly Thr Gly Asp Ala Lys Glu Ile Gly Glu Arg Glu Gly Lys Phe 205 210 215 tat gcc ata aat gtc cca ttg aag gat gga ata gat gac agt agc ttc 844Tyr Ala Ile Asn Val Pro Leu Lys Asp Gly Ile Asp Asp Ser Ser Phe 220 225 230 act cga ctt ttc aag act att att tcc aaa gta gtt gaa aca tat caa 892Thr Arg Leu Phe Lys Thr Ile Ile Ser Lys Val Val Glu Thr Tyr Gln 235 240 245 250 cct ggt gca ata gtt ctg cag tgt gga gca gat tcg ctt gct gga gat 940Pro Gly Ala Ile Val Leu Gln Cys Gly Ala Asp Ser Leu Ala Gly Asp 255 260 265 cgc ttg ggt tgc ttc aat ctc tct att gat ggt cat gct gaa tgt gtt 988Arg Leu Gly Cys Phe Asn Leu Ser Ile Asp Gly His Ala Glu Cys Val 270 275 280 agc ttc gta aag aga ttc aat ttg cca ttg ctg gtc act gga ggt ggg 1036Ser Phe Val Lys Arg Phe Asn Leu Pro Leu Leu Val Thr Gly Gly Gly 285 290 295 gga tac aca aaa gaa aat gtt gct cga tgt tgg act gtt gaa aca gga 1084Gly Tyr Thr Lys Glu Asn Val Ala Arg Cys Trp Thr Val Glu Thr Gly 300 305 310 gtt ctt cta gat aca gag ctt cca aat gag att ccg caa aat gat tat 1132Val Leu Leu Asp Thr Glu Leu Pro Asn Glu Ile Pro Gln Asn Asp Tyr 315 320 325

330 att aaa tac ttt gca cca gaa ttt tct ttg aag gtt cca aat ggg ccg 1180Ile Lys Tyr Phe Ala Pro Glu Phe Ser Leu Lys Val Pro Asn Gly Pro 335 340 345 ata gaa aat ttg aat agt aaa tca tat ctt agc acc att aaa atg caa 1228Ile Glu Asn Leu Asn Ser Lys Ser Tyr Leu Ser Thr Ile Lys Met Gln 350 355 360 gtc ttg gaa aat ctt cgt tgc atc cag cat gct cca agc gta cag atg 1276Val Leu Glu Asn Leu Arg Cys Ile Gln His Ala Pro Ser Val Gln Met 365 370 375 cag gag gtc cct cct gac ttc tac att cca gaa ttc gat gaa gat gag 1324Gln Glu Val Pro Pro Asp Phe Tyr Ile Pro Glu Phe Asp Glu Asp Glu 380 385 390 cag aac cct gat gaa cgc att gat cag cac act caa gac aag cac atc 1372Gln Asn Pro Asp Glu Arg Ile Asp Gln His Thr Gln Asp Lys His Ile 395 400 405 410 cag cgc gat gat gaa tat tat gat ggt gac aat gac aat gat caa atg 1420Gln Arg Asp Asp Glu Tyr Tyr Asp Gly Asp Asn Asp Asn Asp Gln Met 415 420 425 aat att tca tgaagtgcag ttgccgtttg ccttttggcg ggggattgac 1469Asn Ile Ser cgttttaaga gagaaggaaa atgttaatta gacaaacacc tagatgtatc aacaaagcgg 1529tagtactagc ccaagaaact tgtatattta taagattttt attgttttca gttgctaata 1589tatttgctca aagttacatt attaatgatg tttcttccct gtcatttttt tgaatgaaaa 1649tggacggtgt tagagcaact ctgacaacaa gagcttgcat aacattcttt ttacagaaaa 1709aaaaaaaaaa aaaa 172350429PRTGlycine max 50Met Arg Ser Lys Asp Arg Ile Ala Tyr Phe Tyr Asp Gly Asp Val Gly 1 5 10 15 Ser Val Tyr Phe Gly Ala Lys His Pro Met Lys Pro His Arg Leu Cys 20 25 30 Met Thr His His Leu Val Leu Ser Tyr Asp Leu His Lys Lys Met Glu 35 40 45 Ile Tyr Arg Pro His Lys Ala Tyr Pro Val Glu Leu Ala Gln Phe His 50 55 60 Ser Ala Asp Tyr Val Glu Phe Leu Asn Arg Ile Thr Pro Asp Thr Gln 65 70 75 80 His Leu Phe Leu Asn Glu Leu Thr Lys Tyr Asn Leu Gly Glu Asp Cys 85 90 95 Pro Val Phe Asp Asn Leu Phe Glu Phe Cys Gln Ile Tyr Ala Gly Gly 100 105 110 Thr Ile Asp Ala Ala Arg Arg Leu Asn Asn Gln Leu Cys Asp Ile Ala 115 120 125 Ile Asn Trp Ala Gly Gly Leu His His Ala Lys Lys Cys Glu Ala Ser 130 135 140 Gly Phe Cys Tyr Ile Asn Asp Leu Val Leu Gly Ile Leu Glu Leu Leu 145 150 155 160 Lys Tyr His Ala Arg Val Leu Tyr Ile Asp Ile Asp Val His His Gly 165 170 175 Asp Gly Val Glu Glu Ala Phe Tyr Phe Thr Asp Arg Val Met Thr Val 180 185 190 Ser Phe His Lys Tyr Gly Asp Ser Phe Phe Pro Gly Thr Gly Asp Ala 195 200 205 Lys Glu Ile Gly Glu Arg Glu Gly Lys Phe Tyr Ala Ile Asn Val Pro 210 215 220 Leu Lys Asp Gly Ile Asp Asp Ser Ser Phe Thr Arg Leu Phe Lys Thr 225 230 235 240 Ile Ile Ser Lys Val Val Glu Thr Tyr Gln Pro Gly Ala Ile Val Leu 245 250 255 Gln Cys Gly Ala Asp Ser Leu Ala Gly Asp Arg Leu Gly Cys Phe Asn 260 265 270 Leu Ser Ile Asp Gly His Ala Glu Cys Val Ser Phe Val Lys Arg Phe 275 280 285 Asn Leu Pro Leu Leu Val Thr Gly Gly Gly Gly Tyr Thr Lys Glu Asn 290 295 300 Val Ala Arg Cys Trp Thr Val Glu Thr Gly Val Leu Leu Asp Thr Glu 305 310 315 320 Leu Pro Asn Glu Ile Pro Gln Asn Asp Tyr Ile Lys Tyr Phe Ala Pro 325 330 335 Glu Phe Ser Leu Lys Val Pro Asn Gly Pro Ile Glu Asn Leu Asn Ser 340 345 350 Lys Ser Tyr Leu Ser Thr Ile Lys Met Gln Val Leu Glu Asn Leu Arg 355 360 365 Cys Ile Gln His Ala Pro Ser Val Gln Met Gln Glu Val Pro Pro Asp 370 375 380 Phe Tyr Ile Pro Glu Phe Asp Glu Asp Glu Gln Asn Pro Asp Glu Arg 385 390 395 400 Ile Asp Gln His Thr Gln Asp Lys His Ile Gln Arg Asp Asp Glu Tyr 405 410 415 Tyr Asp Gly Asp Asn Asp Asn Asp Gln Met Asn Ile Ser 420 425 511498DNAGlycine maxCDS(10)..(1260) 51ctggtctcc atg cac ctt ctc aaa ttt cct cgc tct cca tct tcc ttc ggg 51 Met His Leu Leu Lys Phe Pro Arg Ser Pro Ser Ser Phe Gly 1 5 10 aat gcg ttc ttt ctg gtg ggt cat cat gtt ttg gac ata aga gtg ttc 99Asn Ala Phe Phe Leu Val Gly His His Val Leu Asp Ile Arg Val Phe 15 20 25 30 cgt aag aac cag aga tgt ttc aga gcg tcc ata tcg tgt tcg gct gtt 147Arg Lys Asn Gln Arg Cys Phe Arg Ala Ser Ile Ser Cys Ser Ala Val 35 40 45 agg aac ggt tct att gag caa cta agt gat gca cgg ctt ata tac tcc 195Arg Asn Gly Ser Ile Glu Gln Leu Ser Asp Ala Arg Leu Ile Tyr Ser 50 55 60 gtc gct cca tct atg ggc cac aac cag gag tct cat cca gaa tca cat 243Val Ala Pro Ser Met Gly His Asn Gln Glu Ser His Pro Glu Ser His 65 70 75 ttt aga gtt ccc gcg att gtc aat gct cta gaa gaa atg cag ctc act 291Phe Arg Val Pro Ala Ile Val Asn Ala Leu Glu Glu Met Gln Leu Thr 80 85 90 tcc aag ttc cgt ggc cca gag gta att gaa ctt caa cat ttt gag cct 339Ser Lys Phe Arg Gly Pro Glu Val Ile Glu Leu Gln His Phe Glu Pro 95 100 105 110 gct tca gtt gat gat att gca agt gtg cat gca aga gcc tat gtt tct 387Ala Ser Val Asp Asp Ile Ala Ser Val His Ala Arg Ala Tyr Val Ser 115 120 125 ggg ctt gaa aag gtt atg gat caa gct gtg gag aaa ggc ctt att ttc 435Gly Leu Glu Lys Val Met Asp Gln Ala Val Glu Lys Gly Leu Ile Phe 130 135 140 ctt gat ggt tca gga cca aca tat gcc act gcc act acc ttc cag gag 483Leu Asp Gly Ser Gly Pro Thr Tyr Ala Thr Ala Thr Thr Phe Gln Glu 145 150 155 tca ata gtt gca gcc ggt gct gga tta gcc tta gtt gac tca gtg gtt 531Ser Ile Val Ala Ala Gly Ala Gly Leu Ala Leu Val Asp Ser Val Val 160 165 170 gca tgt tca aag ata aag gga gat gca ccc act ggt ttt gct ctg ata 579Ala Cys Ser Lys Ile Lys Gly Asp Ala Pro Thr Gly Phe Ala Leu Ile 175 180 185 190 aga cca cca gga cat cac gca gtt cca caa gga cct atg gga ttc tgc 627Arg Pro Pro Gly His His Ala Val Pro Gln Gly Pro Met Gly Phe Cys 195 200 205 att ttt gga aat gtg gcc att gca gcc cgt tat tct cag cgt gtt cat 675Ile Phe Gly Asn Val Ala Ile Ala Ala Arg Tyr Ser Gln Arg Val His 210 215 220 gga ttg aag cgt gtg ttt ata att gac ttt gat gtt cat cat ggg aat 723Gly Leu Lys Arg Val Phe Ile Ile Asp Phe Asp Val His His Gly Asn 225 230 235 gga aca aat gat gct ttc tat gat gat cca gat gta ttt ttc ctt tca 771Gly Thr Asn Asp Ala Phe Tyr Asp Asp Pro Asp Val Phe Phe Leu Ser 240 245 250 ttt cac caa gat gga agc tat ccc ggt act ggt aaa ttt gat gaa gtt 819Phe His Gln Asp Gly Ser Tyr Pro Gly Thr Gly Lys Phe Asp Glu Val 255 260 265 270 gga agt gga gat ggt gaa gga acc aca tta aat ctg cct ctt cct gga 867Gly Ser Gly Asp Gly Glu Gly Thr Thr Leu Asn Leu Pro Leu Pro Gly 275 280 285 ggt tca ggt gat act gct att aga act gtg ttc gat gaa gtc att gta 915Gly Ser Gly Asp Thr Ala Ile Arg Thr Val Phe Asp Glu Val Ile Val 290 295 300 cca tgt gct caa aga ttt aaa cca gac atc att ctt gtt tct gct ggg 963Pro Cys Ala Gln Arg Phe Lys Pro Asp Ile Ile Leu Val Ser Ala Gly 305 310 315 tat gac ggc cac gtg ttg gat cca cta gct aat ctt caa tat aca act 1011Tyr Asp Gly His Val Leu Asp Pro Leu Ala Asn Leu Gln Tyr Thr Thr 320 325 330 gga aca tat tac atg cta gcg tcc agt atc aaa caa cta gcg aaa gat 1059Gly Thr Tyr Tyr Met Leu Ala Ser Ser Ile Lys Gln Leu Ala Lys Asp 335 340 345 350 tta tgt ggg ggc cga tgt gtg ttt ttc ttg gag gga gga tat aat ctg 1107Leu Cys Gly Gly Arg Cys Val Phe Phe Leu Glu Gly Gly Tyr Asn Leu 355 360 365 aag tct ctt tca tat tcc gtg gca gac aca ttc cgt gct ctt ctt ggg 1155Lys Ser Leu Ser Tyr Ser Val Ala Asp Thr Phe Arg Ala Leu Leu Gly 370 375 380 gac cga agc ttg gca tct gag ttt gat aac cct aac att ttg tac gaa 1203Asp Arg Ser Leu Ala Ser Glu Phe Asp Asn Pro Asn Ile Leu Tyr Glu 385 390 395 gag cca tct aca aaa gtt aag caa gct att cag aag ata aaa cac att 1251Glu Pro Ser Thr Lys Val Lys Gln Ala Ile Gln Lys Ile Lys His Ile 400 405 410 cat tcc ctg tgaggtcaaa atagaaactg acatgacaag catctaaatg 1300His Ser Leu 415 tctagcatta ataatttcct tttctgtggg ttcaatctat aagtacatgt ctttacactc 1360ttgggtgggc attctcccac ttatcacata gaagcaaaac cattgtacag gattgcttgt 1420ggtctttgaa cgagtctcca gttacagttt ctatatgcca tgcagccatg catattgatc 1480aaaaaaaaaa aaaaaaaa 149852417PRTGlycine max 52Met His Leu Leu Lys Phe Pro Arg Ser Pro Ser Ser Phe Gly Asn Ala 1 5 10 15 Phe Phe Leu Val Gly His His Val Leu Asp Ile Arg Val Phe Arg Lys 20 25 30 Asn Gln Arg Cys Phe Arg Ala Ser Ile Ser Cys Ser Ala Val Arg Asn 35 40 45 Gly Ser Ile Glu Gln Leu Ser Asp Ala Arg Leu Ile Tyr Ser Val Ala 50 55 60 Pro Ser Met Gly His Asn Gln Glu Ser His Pro Glu Ser His Phe Arg 65 70 75 80 Val Pro Ala Ile Val Asn Ala Leu Glu Glu Met Gln Leu Thr Ser Lys 85 90 95 Phe Arg Gly Pro Glu Val Ile Glu Leu Gln His Phe Glu Pro Ala Ser 100 105 110 Val Asp Asp Ile Ala Ser Val His Ala Arg Ala Tyr Val Ser Gly Leu 115 120 125 Glu Lys Val Met Asp Gln Ala Val Glu Lys Gly Leu Ile Phe Leu Asp 130 135 140 Gly Ser Gly Pro Thr Tyr Ala Thr Ala Thr Thr Phe Gln Glu Ser Ile 145 150 155 160 Val Ala Ala Gly Ala Gly Leu Ala Leu Val Asp Ser Val Val Ala Cys 165 170 175 Ser Lys Ile Lys Gly Asp Ala Pro Thr Gly Phe Ala Leu Ile Arg Pro 180 185 190 Pro Gly His His Ala Val Pro Gln Gly Pro Met Gly Phe Cys Ile Phe 195 200 205 Gly Asn Val Ala Ile Ala Ala Arg Tyr Ser Gln Arg Val His Gly Leu 210 215 220 Lys Arg Val Phe Ile Ile Asp Phe Asp Val His His Gly Asn Gly Thr 225 230 235 240 Asn Asp Ala Phe Tyr Asp Asp Pro Asp Val Phe Phe Leu Ser Phe His 245 250 255 Gln Asp Gly Ser Tyr Pro Gly Thr Gly Lys Phe Asp Glu Val Gly Ser 260 265 270 Gly Asp Gly Glu Gly Thr Thr Leu Asn Leu Pro Leu Pro Gly Gly Ser 275 280 285 Gly Asp Thr Ala Ile Arg Thr Val Phe Asp Glu Val Ile Val Pro Cys 290 295 300 Ala Gln Arg Phe Lys Pro Asp Ile Ile Leu Val Ser Ala Gly Tyr Asp 305 310 315 320 Gly His Val Leu Asp Pro Leu Ala Asn Leu Gln Tyr Thr Thr Gly Thr 325 330 335 Tyr Tyr Met Leu Ala Ser Ser Ile Lys Gln Leu Ala Lys Asp Leu Cys 340 345 350 Gly Gly Arg Cys Val Phe Phe Leu Glu Gly Gly Tyr Asn Leu Lys Ser 355 360 365 Leu Ser Tyr Ser Val Ala Asp Thr Phe Arg Ala Leu Leu Gly Asp Arg 370 375 380 Ser Leu Ala Ser Glu Phe Asp Asn Pro Asn Ile Leu Tyr Glu Glu Pro 385 390 395 400 Ser Thr Lys Val Lys Gln Ala Ile Gln Lys Ile Lys His Ile His Ser 405 410 415 Leu 532238DNATriticum aestivumCDS(77)..(1633) 53ccgagatacc gaaacccaaa cacagaggcg cgcaaacacg cggaggagag aaggcgcctc 60ctccccccac gccgcg atg gac atc tcg gcc ggc ggc ggc ggc aac tcg ctg 112 Met Asp Ile Ser Ala Gly Gly Gly Gly Asn Ser Leu 1 5 10 ccc acc acc ggc gcg gac ggc tcg aag cgc cgc gtc tgc tac ttc tac 160Pro Thr Thr Gly Ala Asp Gly Ser Lys Arg Arg Val Cys Tyr Phe Tyr 15 20 25 gac gcg gag gtg ggc aac tac tac tac ggg cag ggc cac ccg atg aag 208Asp Ala Glu Val Gly Asn Tyr Tyr Tyr Gly Gln Gly His Pro Met Lys 30 35 40 ccg cac cgc atc cgc atg acc cac gcc ctg ctc gcc cac tac ggc ctc 256Pro His Arg Ile Arg Met Thr His Ala Leu Leu Ala His Tyr Gly Leu 45 50 55 60 ctc gac gag atg cag gtg ctc aag ccg cac ccc gcc cgc gac cgc gac 304Leu Asp Glu Met Gln Val Leu Lys Pro His Pro Ala Arg Asp Arg Asp 65 70 75 ctc tgc cgc ttc cac gcc gac gac tac gtc tcc ttc ctc cgc tcc gtc 352Leu Cys Arg Phe His Ala Asp Asp Tyr Val Ser Phe Leu Arg Ser Val 80 85 90 acc ccg gag acg cag cag gac cag atc cgc gcc ctc aag cgc ttc aac 400Thr Pro Glu Thr Gln Gln Asp Gln Ile Arg Ala Leu Lys Arg Phe Asn 95 100 105 gtc ggc gag gac tgc ccc gtc ttc gac ggc ctc tac agc ttc tgc caa 448Val Gly Glu Asp Cys Pro Val Phe Asp Gly Leu Tyr Ser Phe Cys Gln 110 115 120 acc tac gcc ggc ggc tcc gtc ggg ggc gcc gtc aag ctc aac cac ggc 496Thr Tyr Ala Gly Gly Ser Val Gly Gly Ala Val Lys Leu Asn His Gly 125 130 135 140 cac gac atc gcc atc aac tgg gcc ggc ggc ctc cac cac gcc aag aag 544His Asp Ile Ala Ile Asn Trp Ala Gly Gly Leu His His Ala Lys Lys 145 150 155 tgc gag gcc tcc ggc ttc tgc tac gtc aac gac atc gtc ctc gcc atc 592Cys Glu Ala Ser Gly Phe Cys Tyr Val Asn Asp Ile Val Leu Ala Ile 160 165 170 ctc gag ctc ctc aaa tac cac cag cgt gtt ctg tat gtc gat atc gat 640Leu Glu Leu Leu Lys Tyr His Gln Arg Val Leu Tyr Val Asp Ile Asp 175 180 185 att cac cat ggg gac ggc gtg gag gag gcg ttt tac acc acg gac agg 688Ile His His Gly Asp Gly Val Glu Glu Ala Phe Tyr Thr Thr Asp Arg 190 195 200 gtg atg act gtc tca ttc cac aag ttt ggg gat tat ttc cca ggg aca 736Val Met Thr Val Ser Phe His Lys Phe Gly Asp Tyr Phe Pro Gly Thr 205 210 215 220 gga gac att cgt gac gtt ggg cac tcc aag ggg aag tat tat tcc ctg 784Gly Asp Ile Arg Asp Val Gly His Ser Lys Gly Lys Tyr Tyr Ser Leu 225 230 235 aat gtc ccg ttg gat gac ggc atc gac gac gag

agc tat caa tcg ttg 832Asn Val Pro Leu Asp Asp Gly Ile Asp Asp Glu Ser Tyr Gln Ser Leu 240 245 250 ttc aag ccg atc atg ggt aaa gtt atg gag att ttc cgc ccc ggc gcg 880Phe Lys Pro Ile Met Gly Lys Val Met Glu Ile Phe Arg Pro Gly Ala 255 260 265 gtg gta ctc cag tgt gga gca gat tcc tta tcc ggt gac agg ttg ggc 928Val Val Leu Gln Cys Gly Ala Asp Ser Leu Ser Gly Asp Arg Leu Gly 270 275 280 tgc ttc aac cta tcc att aag ggg cac gca gag tgc gtg aga ttc atg 976Cys Phe Asn Leu Ser Ile Lys Gly His Ala Glu Cys Val Arg Phe Met 285 290 295 300 agg tcc ttc aat gtt ccg gtg ttg ctg ctt ggt ggt ggt ggt tat acc 1024Arg Ser Phe Asn Val Pro Val Leu Leu Leu Gly Gly Gly Gly Tyr Thr 305 310 315 ata aga aat gtt gca cga tgt tgg tgc tat gag acg gga gtt gca ctt 1072Ile Arg Asn Val Ala Arg Cys Trp Cys Tyr Glu Thr Gly Val Ala Leu 320 325 330 ggt cat gag cta act gac aag atg ccg cta aat gag cat tat gag tat 1120Gly His Glu Leu Thr Asp Lys Met Pro Leu Asn Glu His Tyr Glu Tyr 335 340 345 ttt ggc cca gat tat act ctt cat gtt gca cca agt aat atg gag aac 1168Phe Gly Pro Asp Tyr Thr Leu His Val Ala Pro Ser Asn Met Glu Asn 350 355 360 aaa aac aca cac cgg cat ttg gat gaa ata aga tca aga ctt ctc gaa 1216Lys Asn Thr His Arg His Leu Asp Glu Ile Arg Ser Arg Leu Leu Glu 365 370 375 380 aat ctt aca aaa ctc cgg cat gct cct agt gtg cag ttt caa gag cga 1264Asn Leu Thr Lys Leu Arg His Ala Pro Ser Val Gln Phe Gln Glu Arg 385 390 395 cct cct gag gcc gag caa cca gag caa gat gag gat caa gag aat cct 1312Pro Pro Glu Ala Glu Gln Pro Glu Gln Asp Glu Asp Gln Glu Asn Pro 400 405 410 gat gaa agg cat cat gct gac tct gat gtg gaa atg gat gat gcc aag 1360Asp Glu Arg His His Ala Asp Ser Asp Val Glu Met Asp Asp Ala Lys 415 420 425 cct ctg gag gac tct gaa agg aga acc agt act cag ggt gcg aga gtt 1408Pro Leu Glu Asp Ser Glu Arg Arg Thr Ser Thr Gln Gly Ala Arg Val 430 435 440 aag aga gaa tct gct gaa act gag gtg aca aca gat cag gat ggt aac 1456Lys Arg Glu Ser Ala Glu Thr Glu Val Thr Thr Asp Gln Asp Gly Asn 445 450 455 460 gga gta gct tca gaa caa gta agg ggc cca gaa cct gtg gct gat gga 1504Gly Val Ala Ser Glu Gln Val Arg Gly Pro Glu Pro Val Ala Asp Gly 465 470 475 gtt ggt tcc tca aaa caa aac cct cct att gat gca agt ccg atg gcc 1552Val Gly Ser Ser Lys Gln Asn Pro Pro Ile Asp Ala Ser Pro Met Ala 480 485 490 ata gac ggg cca gct gtt gtc agg gct gaa cca gag agg tca aac aaa 1600Ile Asp Gly Pro Ala Val Val Arg Ala Glu Pro Glu Arg Ser Asn Lys 495 500 505 tta cag gaa caa caa gca ttg cat cag aaa cca tgatcatcca ccttaacgta 1653Leu Gln Glu Gln Gln Ala Leu His Gln Lys Pro 510 515 gcaactgatg catctgtgca gcccattgac tccattggac gagccaggac agtctgtaag 1713ctgtagcata gttcaaacaa tctactgcac caacaagtta gctaccagaa tccaagatgg 1773tccgtgtgcc ttgagagcag ttgaagcttt gtgcactata ttcgtgactt tgacacaaat 1833gatagttgat tcgttaatgc catttttagc actaatttaa gtctggggcc tgtagagcaa 1893ccaagttagt aatgcaatac attccaatca aatgcttccg gggagatgac ctggtgtatg 1953tgacttagca gtgtatagct aggcgaaggc ttccttttgg ttggagctgt tttttctcat 2013cgcataaggg tcttcttatg gtctgttagg aagaatgccg cttactgcgt tttcggtgtc 2073aagtctgttc cattgaagaa aagttggatt cgtaatgtat tcccattgat ggattgtctc 2133tttattgagt catcattgtt cttgtaacat tctagttggg gaaataattg tggcctttgt 2193attgtaatgt tcaaacttga gaagactaaa aaaaaaaaaa aaaaa 223854519PRTTriticum aestivum 54Met Asp Ile Ser Ala Gly Gly Gly Gly Asn Ser Leu Pro Thr Thr Gly 1 5 10 15 Ala Asp Gly Ser Lys Arg Arg Val Cys Tyr Phe Tyr Asp Ala Glu Val 20 25 30 Gly Asn Tyr Tyr Tyr Gly Gln Gly His Pro Met Lys Pro His Arg Ile 35 40 45 Arg Met Thr His Ala Leu Leu Ala His Tyr Gly Leu Leu Asp Glu Met 50 55 60 Gln Val Leu Lys Pro His Pro Ala Arg Asp Arg Asp Leu Cys Arg Phe 65 70 75 80 His Ala Asp Asp Tyr Val Ser Phe Leu Arg Ser Val Thr Pro Glu Thr 85 90 95 Gln Gln Asp Gln Ile Arg Ala Leu Lys Arg Phe Asn Val Gly Glu Asp 100 105 110 Cys Pro Val Phe Asp Gly Leu Tyr Ser Phe Cys Gln Thr Tyr Ala Gly 115 120 125 Gly Ser Val Gly Gly Ala Val Lys Leu Asn His Gly His Asp Ile Ala 130 135 140 Ile Asn Trp Ala Gly Gly Leu His His Ala Lys Lys Cys Glu Ala Ser 145 150 155 160 Gly Phe Cys Tyr Val Asn Asp Ile Val Leu Ala Ile Leu Glu Leu Leu 165 170 175 Lys Tyr His Gln Arg Val Leu Tyr Val Asp Ile Asp Ile His His Gly 180 185 190 Asp Gly Val Glu Glu Ala Phe Tyr Thr Thr Asp Arg Val Met Thr Val 195 200 205 Ser Phe His Lys Phe Gly Asp Tyr Phe Pro Gly Thr Gly Asp Ile Arg 210 215 220 Asp Val Gly His Ser Lys Gly Lys Tyr Tyr Ser Leu Asn Val Pro Leu 225 230 235 240 Asp Asp Gly Ile Asp Asp Glu Ser Tyr Gln Ser Leu Phe Lys Pro Ile 245 250 255 Met Gly Lys Val Met Glu Ile Phe Arg Pro Gly Ala Val Val Leu Gln 260 265 270 Cys Gly Ala Asp Ser Leu Ser Gly Asp Arg Leu Gly Cys Phe Asn Leu 275 280 285 Ser Ile Lys Gly His Ala Glu Cys Val Arg Phe Met Arg Ser Phe Asn 290 295 300 Val Pro Val Leu Leu Leu Gly Gly Gly Gly Tyr Thr Ile Arg Asn Val 305 310 315 320 Ala Arg Cys Trp Cys Tyr Glu Thr Gly Val Ala Leu Gly His Glu Leu 325 330 335 Thr Asp Lys Met Pro Leu Asn Glu His Tyr Glu Tyr Phe Gly Pro Asp 340 345 350 Tyr Thr Leu His Val Ala Pro Ser Asn Met Glu Asn Lys Asn Thr His 355 360 365 Arg His Leu Asp Glu Ile Arg Ser Arg Leu Leu Glu Asn Leu Thr Lys 370 375 380 Leu Arg His Ala Pro Ser Val Gln Phe Gln Glu Arg Pro Pro Glu Ala 385 390 395 400 Glu Gln Pro Glu Gln Asp Glu Asp Gln Glu Asn Pro Asp Glu Arg His 405 410 415 His Ala Asp Ser Asp Val Glu Met Asp Asp Ala Lys Pro Leu Glu Asp 420 425 430 Ser Glu Arg Arg Thr Ser Thr Gln Gly Ala Arg Val Lys Arg Glu Ser 435 440 445 Ala Glu Thr Glu Val Thr Thr Asp Gln Asp Gly Asn Gly Val Ala Ser 450 455 460 Glu Gln Val Arg Gly Pro Glu Pro Val Ala Asp Gly Val Gly Ser Ser 465 470 475 480 Lys Gln Asn Pro Pro Ile Asp Ala Ser Pro Met Ala Ile Asp Gly Pro 485 490 495 Ala Val Val Arg Ala Glu Pro Glu Arg Ser Asn Lys Leu Gln Glu Gln 500 505 510 Gln Ala Leu His Gln Lys Pro 515

Claims

1-22. (canceled)

23. A transgenic plant cell or a transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 239 of SEQ ID NO:34.

24. An isolated polynucleotide having a sequence selected from the group consisting of a) a polynucleotide having a sequence comprising nucleotides 16 to 732 of SEQ ID NO:33; and b) a polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 239 of SEQ ID NO:34.

25. An isolated polypeptide having a sequence comprising amino acids 1 to 239 of SEQ ID NO:34.

26. A method of producing a transgenic plant comprising the steps of: a) introducing into a plant cell an expression vector comprising a polynucleotide having a sequence selected from the group consisting of: (i) a polynucleotide having a sequence comprising nucleotides 16 to 732 of SEQ ID NO:33; and (ii) a polynucleotide which encodes a polypeptide having a sequence comprising amino acids 1 to 239 of SEQ ID NO:34; and b) generating from the plant cell a transgenic plant that expresses the polynucleotide.

27. A method of increasing a plant's growth or yield under normal or water-limited conditions or increasing a plant's tolerance to an environmental stress, the method comprising the steps of: (a) inserting into an expression vector a polynucleotide which encodes a polypeptide having a sequence selected from the group consisting of: (i) a polynucleotide having a sequence comprising nucleotides 16 to 732 of SEQ ID NO: 33 and (ii) a polynucleotide which encodes a polypeptide having a sequence comprising amino acids 1 to 239 of SEQ ID NO: 34; and (b) introducing the expression vector into the plant.