THERAPEUTIC AGENT FOR SPINAL CORD INJURIES

Abstract

Disclosed is a therapeutic agent effective for the fundamental treatment of a spinal cord injury and a demyelinating disease. Specifically disclosed are a therapeutic agent for a spinal cord injury and a therapeutic agent for a demyelinating disease, each of which comprises an HGF protein as an active ingredient.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of and claims the benefit of priority to PCT International Application Number PCT/JP2008/053557, filed Feb. 28, 2008, which claims the benefit of priority of PCT international Application number PCT/JP2007/053804, filed Feb. 28, 2007; all of which are hereby expressly incorporated by reference in their entireties.

BACKGROUND OF THE INVENTION

[0002] 1. Field of the Invention

[0003] The present invention relates to a therapeutic agent for spinal cord injuries, and more particularly to a therapeutic agent for spinal cord injuries in which hepatocyte growth factor (abbreviated below as "HGF") protein serves as the active ingredient. The invention also relates to a therapeutic agent for demyelinating diseases in which HGF protein serves as the active ingredient

[0004] 2. Description of the Related Art

[0005] The term "spinal cord injury" (SCI) refers to a clinical state that presents peripheral motor, sensory and autonomous nervous system paralysis below the site of injury to the spinal cord parenchyma from trauma such as dislocation-fracture of the spine as a result of, for example, a traffic accident or a fall from a high place.

[0006] The number of spinal cord injury patients is currently about 100,000 in Japan, and some 250,000 in the United States. Each year, the number of such patients increases by at least 5,000 in Japan and at least 10,000 in the U.S.

[0007] With recent advances in medical care, the survival rate following injury has risen, and remarkable advances have been made also in methods of reconstructive surgery for spinal cord injuries that are intended to check the progression of disability. As a consequence, success is starting to be achieved in checking secondary neurological deterioration as well. In addition, owing to improvements in rehabilitation technology and the development of supportive devices (electric-powered wheelchairs, etc.), the activities of daily living (ADL) of the patient have improved. However, because of the absence of effective methods for fundamentally treating basic spinal cord injuries (i.e., nerve protection from neurological injury and nerve regeneration), there exist today large numbers of such patients who are unable to relieve themselves, do manual labor or walk without the assistance of others.

[0008] HGF was initially identified as a powerful mitogen for mature hepatocytes, and in 1989 was genetically cloned (Biochem. Biophys. Res. Commun. 122, 1450-1459 (1984) and Nature 342, 440-443 (1989)). Although discovered as hepatocyte growth factor, from numerous recent studies in expression and functional analysis that include knockout/knockin mouse techniques, HGF has also been found to be a novel neurotrophic factor (Nat. Neurosci. 2, 213-217 (1999) and Clin. Chim Acta., 327, 1-23 (2003)).

[0009] In WO 03/045439, working examples are described in which the effects of the HGF gene on Parkinson's disease model rats were behaviorally and histologically investigated. The experimental results presented therein indicate that the prior administration of HGF gene had the effect of protecting dopamine neurons in the mesencephalic substantia nigra from the neurotoxin 6-hydroxydopamine (6-OHDA). WO 03/045439 also states that, based on these experimental results, the HGF gene can be used in the treatment of not only Parkinson's disease, but other neurological disorders as well, including Alzheimer disease, spinocerebellar degeneration, multiple sclerosis, striatonigral degeneration (SND), spinal muscular atrophy (SMA), Huntington chorea, Shy-Drager syndrome, Charcot-Marie-Tooth disease (CMT), Friedreich ataxia, myasthenia gravis, moyamoya disease, amyloidosis, pick disease, subacute myeloopticoneuropathy, dermatomyositis/polymyositis, Creutzfeldt-Jacov disease, Behcet syndrome, systemic lupus erythematosus (SLE), sarcoidosis, periarteritis nodosa (PN), ossification of posterior longitudinal ligament, diffuse spinal canal stenosis, mixed connective tissue disease (MCTD), diabetic peripheral neuritis and ischemic cerebrovascular disorders (e.g., cerebral infarction, cerebral hemorrhaging), and moreover mentions spinal cord injuries as one such type of neurological disorder.

[0010] However, 6-OHDA is a special synthetic toxin which has a specific effect on neurons that synthesize catecholamines (specifically, noradrenaline-, adrenaline- and dopamine-producing neurons), and does not exhibit any toxicity against the neurons which are reportedly degenerated or killed in most of the diseases listed above. Therefore, it is impossible to predict the effects on the above disorders, including spinal cord injuries, from the neuronal cell death-suppressing effects by 6-OHDA. In addition, WO 03/045439 makes no mention of the therapeutic effects of administering HGF protein.

[0011] The Journal of the Japanese Orthopaedic Association, Vol. 79, No. 8, pS764 (Aug. 25, 2005) mentions that, when a virus vector containing the HGF gene (an HGF-expressing virus vector) was injected into the spinal cord of rats at the tenth thoracic vertebra and a vertebral crushing injury was then created at the same site, the recovery of lower limb motor function was observed in subsequent evaluations of motor function.

[0012] Yet, in spite of the fact that spinal cord injuries generally arise from external trauma suffered in accidents and the like, in The Journal of the Japanese Orthopaedic Association, Vol. 79, No. 8, pS764 (Aug. 25, 2005), the HGF-expressing virus vector was injected 3 days prior to the thoracic vertebral crushing injury. Clearly, it is impossible to predict in this way the occurrence of an accident and the site of injury and to locally administer HGF-expressing virus vector beforehand.

[0013] Moreover, the condition of a spinal cord injury patient is likely to be unstable for 72 hours following the trauma, which may make it very difficult to insert a catheter for intrathecal administration. Determining the proper period of administration is thus important.

[0014] In addition, there are a number of conceivable problems, such as the difficultly of controlling the amount of protein expressed in conventional HGF gene therapy, the danger with some gene expression vectors of triggering an immune response with repeated administration, and the possibility with some gene expression vectors of introducing genes into the genome.

[0015] The nerve fibers of myelinated nerves, including the nerves of the spinal cord, are covered with a sheath composed of a layer of lipoprotein called myelin. This myelin sheath functions as an insulator for the nerve fibers, enabling saltatory conduction by the myelinated nerve. The destruction of this myelin sheath is referred to as demyelination. When demyelination occurs, a variety of neurological symptoms arise due to a dramatic slowing of neurotransmission. Diseases accompanied by such demyelination are generally referred to as demyelinating diseases, and typically include, for example, multiple sclerosis. Spinal cord injuries, too, are generally accompanied by demyelination.

[0016] Multiple sclerosis is a slowly progressing central nervous system disease characterized by the formation of disseminated demyelinating plaques. The incidence of multiple sclerosis is about 50 to 100 cases per 100,000 people in Europe and the United States, and is about 1 to 5 cases per 100,000 people in Japan. The symptoms vary widely from individual to individual, and may include loss of vision, double vision, nystagmus, articulation disorders, weakness, abnormal sensations, bladder problems and mood swings. The disease progresses with the repeated remission and resumption of such symptoms. The cause, while not yet determined, is suspected to be an immunological abnormality. Hence, as with other demyelinating diseases, no fundamental treatment currently exists.

[0017] As indicated above, although methods involving the injection of an HGF gene-containing virus vector (HGF expression virus vector) are known, it is also known that viruses such as a herpes virus (HSV) or an adenovirus produce a concentration-dependent inflammatory reaction in the brain when such viruses are introduced into the brain, inviting demyelination (WO 05/100577).

[0018] Therefore, from this perspective as well, treatment methods involving the use of an HGF gene-containing virus vector clearly do not constitute a fundamental approach toward the treatment of demyelinating disease. A desire thus exists for the establishment of a method of treatment that does not invite demyelination.

SUMMARY OF THE INVENTION

Problems to be Solved by the Invention

[0019] An object of the invention is to provide an agent which is capable of treating spinal cord injuries and demyelinating diseases by a simple and convenient method that does not involve the use of a gene.

Means for Solving the Problems

[0020] The inventors have conducted extensive investigations in order to overcome the above problems. As a result, they have discovered that HGF protein has the most highly sought after functional regenerating effects in spinal cord injury treatment, including a demyelination inhibiting effect and a 5HT nerve regenerating effect, making HGF protein useful as a therapeutic agent for spinal cord injuries. Moreover, the inventors have also found that HGF protein is useful as a therapeutic agent for demyelinating diseases. These discoveries ultimately led to the present invention.

[0021] Accordingly the invention relates to:

[0022] (1) a therapeutic agent for treating spinal cord injuries, comprising HGF protein as an active ingredient;

[0023] (2) the therapeutic agent according to (1) above, wherein the HGF protein is a protein having the amino acid sequence of SEQ ID NO: 1 or 2, a protein having substantially the same amino acid sequence as the amino acid sequence of SEQ ID No: 1 or 2 and having substantially the same activity as HGF, or a peptide which is a partial peptide of one of said proteins and has substantially the same activity as HGF;

[0024] (3) the therapeutic agent according to (1) above, wherein the HGF protein is a protein having the amino acid sequence of SEQ ID NO: 2;

[0025] (4) the therapeutic agent according to any of (1) to (3) above, wherein the agent is adapted for localized use at a site of spinal cord injury;

[0026] (5) the therapeutic agent according to (4) above, wherein the agent is in the form of an injectable preparation for intrathecal administration;

[0027] (6) the therapeutic agent according to (4) above, wherein the agent is in the form of an injectable preparation for intrathecal administration by a sustained-release pump;

[0028] (7) the therapeutic agent according to any of (1) to (6) above, wherein the agent is for inhibiting spinal cord nerve demyelination;

[0029] (8) a therapeutic agent for treating spinal cord injuries, which comprises HGF protein as an active ingredient, and which is administered within 2 weeks following a spinal cord injury;

[0030] (9) a therapeutic agent for treating spinal cord injuries, which comprises HGF protein as an active ingredient, and which is administered within 4 days following a spinal cord injury;

[0031] (10) a method for treating spinal cord injuries, the method being comprised of administering an effective dose of HGF protein to a spinal cord injury patient;

[0032] (11) a use of HGF protein for manufacturing an agent for treating spinal cord injuries;

[0033] (12) an HGF protein for treating spinal cord injuries;

[0034] (13) a therapeutic agent for treating a demyelinating disease, comprising HGF protein as an active ingredient;

[0035] (14) the therapeutic agent according to (13) above, wherein the demyelinating disease is a disease selected from among multiple sclerosis, Devic disease, Balo's concentric sclerosis, acute disseminated encephalomyelitis (ADEM), Schilder disease, subacute sclerosing panencephalitis (SSPE), progressive multifocal leukoencephalopathy (PML), Binswanger disease, hypoxic encephalopathy, central pontine myelinolysis, Guillain-Barre syndrome, Fischer syndrome and chronic inflammatory demyelinating polyradiculoneuropathy (CIDP);

[0036] (15) the therapeutic agent according to (13) or (14) above, wherein the HGF protein is a protein having the amino acid sequence of SEQ ID NO: 1 or 2, a protein having substantially the same amino acid sequence as the amino acid sequence of SEQ ID NO: 1 or 2 and having substantially the same activity as HGF, or a peptide which is a partial peptide of one of said proteins and has substantially the same activity as HGF;

[0037] (16) the therapeutic agent according to (13) or (14) above, wherein the HGF protein is a protein having the amino acid sequence of SEQ ID NO: 2;

[0038] (17) the therapeutic agent according to any of (13) to (16) above, wherein the agent is adapted for localized use at a site of disease;

[0039] (18) the therapeutic agent according to (17) above, wherein the agent is in the form of an injectable preparation for intrathecal administration;

[0040] (19) the therapeutic agent according to (17) above, wherein the agent is in the form of an injectable preparation for intrathecal administration by a sustained-release pump;

[0041] (20) a method for treating a demyelinating disease, the method being comprised of administering an effective dose of HGF protein to a patient with a demyelinating disease;

[0042] (21) the use of HGF protein for manufacturing a therapeutic agent for treating a demyelinating disease; and

[0043] (22) an HGF protein for treating a demyelinating disease.

Advantageous Effect of the Invention

[0044] The therapeutic agent of the invention produces outstanding therapeutic effects against spinal cord injuries and demyelinating diseases. The therapeutic agent of the invention also has the advantage that it is free from the problems associated with gene therapy. In addition, the therapeutic agent of the invention has the advantage that it effectively inhibits and treats the demyelination of myelinated nerves that occurs in spinal cord injuries and demyelinating diseases (e.g., multiple sclerosis). Furthermore, since the therapeutic agent of the invention does not require the use of a virus vector such as HSV or an adenovirus, it does not invite demyelination. A further advantage of the therapeutic agent of the invention is that, unlike gene therapy, the amount supplied or dose of the active ingredient HGF can be easily adjusted, in addition to which the time of administration can be adjusted and administration can be carried out either repeatedly or continuously.

BRIEF DESCRIPTION OF THE DRAWINGS

[0045] FIG. 1 shows Hematoxylin-Eosin (HE) stained images of spinal cord tissue following a spinal cord injury from an HGF protein group given 200 μg/2 weeks of HGF protein starting immediately after spinal cord injury, and of spinal cord tissue from a control group.

[0046] FIG. 2 shows Luxol Fast Blue (LFB) stained images of spinal cord tissue following a spinal cord injury from an HGF protein group given 200 μg/2 weeks of HGF protein starting immediately after spinal cord injury, and of spinal cord tissue from a control group.

[0047] FIG. 3 shows 5-Hydroxytryptamine (5HT) stained images of spinal cord tissue following a spinal cord injury from an HGF protein group given 200 μg/2 weeks of HGF protein starting immediately after spinal cord injury, and of spinal cord tissue from a control group.

[0048] FIG. 4 shows 5HT and Growth associated protein-43 (GAP43) stained images of spinal cord tissue following a spinal cord injury from an HGF protein group given 200 μg/2 weeks of HGF protein starting immediately after spinal cord injury.

[0049] FIG. 5 is a line graph showing the Basso-Beattie-Bresnahan (BBB) scores following a spinal cord injury for an HGF protein group given 200 μg/2 weeks of HGF protein starting immediately after spinal cord injury and for a control group. In the graph, the arrow indicates the HGF protein or PBS dosing period.

[0050] FIG. 6 is a line graph showing the BBB scores following a spinal cord injury for an HGF protein group given 400 μg/4 weeks of HGF protein starting immediately after spinal cord injury and for a control group. In the graph, the arrow indicates the HGF protein or PBS dosing period.

[0051] FIG. 7 is a line graph showing the BBB scores following a spinal cord injury for an HGF protein group given 400 μg/4 weeks of HGF protein starting 4 days after spinal cord injury and for a control group. In the graph, the arrow indicates the HGF protein or PBS dosing period.

[0052] FIG. 8 is a line graph showing the BBB scores following a spinal cord injury for an HGF protein group given 400 μg/4 weeks of HGF protein starting 2 weeks after spinal cord injury and for a control group. In the graph, the arrow indicates the HGF protein or PBS dosing period.

[0053] FIG. 9 is a line graph showing the BBB scores following a spinal cord injury for an HGF protein group given 400 μg/4 weeks of HGF protein starting 8 weeks after spinal cord injury and for a control group. In the graph, the arrow indicates the HGF protein or PBS dosing period.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

[0054] The HGF protein used in the present invention is a known substance. HGF protein prepared by any of various methods may be used, provided it has been purified to a degree that enables its use as a medication. HGF protein can be prepared by, for example, growing primary culture cells or cells from an established cell line that produce HGF protein, followed by separation and purification of the HGF protein from the culture supernatant. Alternatively, use may be made of a genetic engineering technique which entails integrating a gene that encodes the HGF protein into a suitable vector, inserting the vector into a suitable host cell to effect transformation, and obtaining the desired recombinant HGF protein from a culture supernatant of the transformant (e.g., see Japanese Patent Application Laid-open No. H5-111382; and Biochem. Biophys. Res. Commun. Vol. 163, p. 967 (1989)). The host cell is not subject to any particular limitation. For example, suitable use may be made of any of various types of host cells hitherto employed in genetic engineering techniques, such as Escherichia coli, yeasts or animal cells. The HGF protein thus obtained, so long as it has substantially the same activity as HGF protein of natural origin, may have on the amino acid sequence thereof one or more (e.g., from 1 to 8; the same applies below) substituted, deleted or added amino acids, or may similarly have substituted, deleted or added sugar chains. Examples of such HGF proteins include the five-amino-acid-deleted-type HGF protein described below. Here, with regard to the amino acid sequence, the phrase "one or more substituted, deleted or added amino acid" means that a number (from one to a plurality) of amino acids have been substituted, deleted, or added by a known technical method such as a genetic engineering technique or site-specific mutagenesis, or naturally. The HGF protein having substituted, deleted or added sugar chains may be, for example, an HGF protein obtained by treating with enzyme or the like a sugar chain added to a natural HGF protein, an HGF protein in which the amino acid sequence at the sugar chain addition site has been altered so that sugar chain addition does not occur, or an HGF protein in which the amino acid sequence has been altered so that a sugar chain is added at a different site than the natural sugar chain addition site.

[0055] In addition, proteins having at least about 80% homology, preferably at least about 90%, more preferably at least about 95% homology with the amino acid sequence of the HGF protein, and substantially acting as HGF may be included. In connection with the above-mentioned amino acid sequences, "homology" refers to the degree of agreement in the amino acid residues making up the respective amino acid sequences in comparison of the primary structures of proteins.

[0056] Examples of the above HGF proteins include the amino acid sequences of SEQ ID NO: 1 and 2. The HGF protein of SEQ ID NO: 2 is a five-amino-acid-deleted-type HGF protein in which the five amino acid residues from amino acids 161 to 165 on the amino acid sequence shown in SEQ ID NO: 1 are deleted. The protein having the amino acid sequence of SEQ ID NO: 1 or 2 is a natural HGF protein of human origin which has the mitogen and motogen activities of HGF.

[0057] Proteins containing an amino acid sequence that is substantially the same as the amino acid sequence of SEQ ID NO: 1 or 2 are proteins containing an amino acid sequence with at least about 80%, preferably at least about 90%, and more preferably at least about 95% identity with the amino acid sequence of SEQ ID NO: 1 or 2. Preferred examples include proteins which act as HGF and have, relative to the amino acid sequence of SEQ ID NO: 1 or 2, an amino acid sequence wherein from one to a plurality of amino acid residues have been inserted or deleted, an amino acid sequence wherein from one to a plurality of amino acid residues have been substituted with other amino acid residues, or an amino acid sequence wherein from one to a plurality of amino acid residues have been modified. The inserted or substituted amino acids may be nonnatural amino acids other than the 20 types of amino acids encoded by genes. The nonnatural amino acids may be any compound having an amino group and a carboxyl group, such as γ-aminobutyric acid. These proteins may be used alone or as mixtures thereof. Examples of proteins containing an amino acid sequence that is substantially the same as the amino acid sequence of SEQ ID NO: 1 or 2 include, but are not limited to, the human HGF deposited in the NCBI database (NCBI-GenBank Flat File Release 164.0) under Accession Nos. BAA14348 and AAC71655.

[0058] HGF proteins that may be used in the present invention are preferably the above-described proteins of human origin for human application while use may also be made of HGF proteins from mammals other than man (e.g., monkey, cow, horse, pig, sheep, dog, cat, rat, mouse, rabbit, hamster, guinea pig, and chimpanzee). Illustrative examples of such HGF proteins include, but are not limited to, the following deposited in, for instance, the NCBI database: mouse HGF (e.g., Accession Nos. AAB31855, NP_--034557, BAA01065, BAA01064), rat HGF (e.g., Accession No. NP_--58713 (the protein having the amino acid sequence shown in SEQ ID NO: 3)), cow HGF (e.g., Accession Nos. NP_--001026921, BAD02475), cat HGF (e.g., Accession Nos. NP_--001009830, BAC10545, BAB21499), dog HGF (e.g., Accession Nos. NP_--001002964, BAC57560), and chimpanzee HGF (e.g., Accession No. XP_--519174).

[0059] The HGF protein used in the present invention may have a carboxyl group (--COOH), a carboxylate group (--COO.sup.-), an amide group (--CONH₂) or an ester group (--COOR) at the C-terminus. Here, the R in the ester is exemplified by C_1-6 alkyl groups such as methyl, ethyl, n-propyl, isopropyl and n-butyl; C_3-8 cycloalkyl groups such as cyclopentyl and cyclohexyl; C_6-12 aryl groups such as phenyl and α-naphthyl; C_7-14 aralkyl groups, including phenyl-C_1-2 alkyl groups such as benzyl and phenethyl, and α-naphthyl-C_1-2 alkyl groups such as α-naphthylmethyl; and pivaroyloxymethyl groups. The HGF protein of the present invention also includes an HGF protein having a carboxyl group (or carboxylate) at a site other than the C-terminus when the carboxyl group has been amidated or esterified. The ester in this case may be, for example, the above-mentioned C-terminal esters. HGF proteins which may be used in the invention include also any of the above-mentioned proteins wherein the amino group in the N-terminal methionine residue is protected with a protecting group (e.g., C_1-6 acyl groups, including formyl and C_2-6 alkanoyl groups such as acetyl), wherein the glutamyl group formed by in vivo cleavage of the N-terminus is converted to pyroglutamic acid, or wherein functional groups (e.g., --OH, --SH, amino group, imidazole group, indole group, guanidine group) on the side chains of amino acids in the molecule are protected with suitable protecting groups (e.g., C_1-6 acyl groups, including formyl and C_2-6 alkanoyl groups such as acetyl), and also complex proteins such as glycoproteins obtained by the bonding of sugar chains.

[0060] The HGF protein used in the invention may be in the form of a partial peptide thereof (sometimes referred to below simply as a "partial peptide"). Examples of such partial peptides include any protein that is a partial peptide of the above-mentioned HGF proteins and has substantially the same activity as HGF. In the present invention, preferred partial peptides include peptides containing an amino acid sequence of at least about 20, preferably at least about 50, and more preferably at least about 100 amino acids, from the amino acid sequence making up the above-mentioned HGF protein. Specific preferred examples include the peptide having the amino acid sequence from amino acid 32 to amino acid 210 starting on the N-terminal side of the human HGF amino acid sequence of SEQ ID NO: 1 (the sequence from the N-terminal hairpin loop on HGF to the first kringle domain), and the peptide having the amino acid sequence from amino acid 32 to amino acid 288 starting on the N-terminal side of the human HGF amino acid sequence of SEQ ID NO: 1 (the sequence from the N-terminal hairpin loop on HGF to the second kringle domain). In the partial peptide of the invention, the C-terminus may be a carboxyl group (--COOH), a carboxylate group (--COO.sup.-), an amide group (--CONH₂) or an ester group (--COOR). Moreover, the partial peptide, as with the above-mentioned HGF protein, encompasses partial peptides in which an amino group on the methionine residue at the N-terminus is protected with a protecting group, partial peptides in which Gln formed by in vivo cleavage of the N-terminus is converted to pyroglutamic acid, partial peptides in which functional groups on side chains of amino acids in the molecule are protected with suitable protecting groups, and also complex peptides such as glycopeptides obtained by the bonding of sugar chains.

[0061] Salts of the HGF proteins (including those in the form of partial peptides) that may be used in the invention are physiologically allowable salts with an acid or base. Physiologically allowable acid addition salts are especially preferred. Illustrative examples of such salts include salts with inorganic acids (e.g., hydrochloric acid, phosphoric acid, hydrobromic acid, sulfuric acid), and salts with organic acids (e.g., acetic acid, formic acid, propionic acid, fumaric acid, maleic acid, succinic acid, tartaric acid, citric acid, malic acid, oxalic acid, benzoic acid, methanesulfonic acid, benzenesulfonic acid).

[0062] When the HGF protein used in the invention is in the form of a partial peptide, this partial peptide may be prepared in accordance with a known peptide synthesis method or by cleaving an HGF protein with a suitable peptidase. Examples of suitable peptide synthesis methods include solid-phase synthesis and liquid-phase synthesis. In cases where partial peptides or amino acids capable of constituting the HGF protein are condensed with the remaining portions and the resulting product has protecting groups, the target peptide can be prepared by removing the protecting groups. Known condensation methods and methods of removing the protecting groups include those described by, for example, M. Bodanszky and M. A. Ondetti in Peptide Synthesis (Interscience Publishers: New York, 1966), and by Schroeder and Luebke in The Peptide (Academic Press: New York, 1965). Following the reaction, a partial peptide of the HGF protein can be purified and isolated by a conventional method of purification, such as a combination of solvent extraction, distillation, column chromatography, liquid chromatography and recrystallization. When the partial peptide obtained by the above method is a free acid or base, it can be converted into a suitable salt by a known method. Conversely, when it is obtained as a salt, it can be converted into a free acid or base by a known method.

[0063] The HGF protein used in the invention is preferably one of human origin for human application while the use of HGF protein derived from mammals other than man may also be used. An example of a suitable HGF protein of rat origin is shown in SEQ ID NO: 3.

[0064] The therapeutic agents of the invention, which are agents for treating spinal cord injuries and agents for treating demyelinating diseases, may be used in all neurological disorders accompanied by spin cord injury or demyelination. Specific examples include multiple sclerosis (MS), Device disease, Balo's concentric sclerosis, acute disseminated encephalomyelitis (ADEM), Schilder disease, subacute sclerosing panencephalitis (SSPE), progressive multifocal leukoencephalopathy (PML), Binswanger disease, hypoxic encephalopathy, central pontine myelinolysis, Guillain-Barre syndrome, Fischer syndrome, and chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). Spinal cord injuries with associated demyelination are also encompassed herein.

[0065] The therapeutic agents of the invention (agents for treating spinal cord injuries and agents for treating demyelinating diseases) may be employed in not only humans, but also mammals other than humans (e.g., monkeys, cows, horses, pigs, sheep, dogs, and cats).

[0066] When the therapeutic agents of the invention (agents for treating spinal cord injuries and agents for treating demyelinating diseases) are to be administered to human or animal patients, they may be prepared in any of various dosage forms, such as liquid medications and solid medications. Generally, the HGF protein by itself or together with a conventional carrier is prepared in the form of, for example, an injectable drug, a spray, or a sustained-release preparation (e.g., depot preparations). The injectable drug may be an aqueous injectable drug or an oil-soluble injectable drug. If the injectable drug is an aqueous injectable drug, preparation may be carried out in accordance with a known method, such as by dissolving the HGF protein in a solution obtained by optionally adding, to an aqueous solvent (e.g., water for injection, purified water), pharmaceutically acceptable excipients, for example, tonicity agents (e.g., sodium chloride, potassium chloride, glycerin, mannitol, sorbitol, boric acid, borax, glucose, propylene glycol), buffers (e.g., phosphate buffer, acetate buffer, borate buffer, carbonate buffer, citrate buffer, Tris buffer, glutamate buffer, epsilon-aminocaproate buffer), preservatives (e.g., methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate, chlorobutanol, benzyl alcohol, benzalkonium chloride, sodium dehydroacetate, edetate sodium, boric acid, borax), thickeners (e.g., hydroxyethyl cellulose, hydroxypropyl cellulose, polyvinyl alcohol, polyethylene glycol), stabilizers (e.g., sodium bisulfite, sodium thiosulfate, edetate sodium, sodium citrate, ascorbic acid, dibutylhydroxytoluene) and pH adjustors (e.g., hydrochloric acid, sodium hydroxide, phosphoric acid, acetic acid), followed by filtration with a filter or the like and sterilization, then filling into a sterile container. A suitable dissolution aid, such as an alcohol (e.g., ethanol), a polyalcohol (e.g., propylene glycol, polyethylene glycol), or a nonionic surfactant (e.g., polysorbate 80, polyoxyethylene-hardened castor oil 50) may also be used. If the injectable drug is an oil-soluble injectable drug, sesame oil or soybean oil may be used as the oleaginous solvent, and benzyl benzoate or benzyl alcohol may be use as the dissolution aid. The injectable drug that has been prepared is generally filled into an ampule or vial. The HGF protein content in the injectable drug is adjusted to generally from about 0.0002 to about 2.0 w/v %, preferably from about 0.001 to about 1.0 w/v %, and more preferably from about 0.01 to about 0.5 w/v %. Also, it is desirable for liquid preparations such as injectable drugs to be preserved by freezing or to be preserved after the removal of moisture by freeze drying or the like. At the time of use, distilled water for injection or the like is added to the freeze-dried preparation so as to redissolve the drug and prepare it for use.

[0067] Sprays may also be prepared in accordance with conventional practice for medicinal preparations. In the case of production as a spray, any additives commonly used in preparations to be inhaled may be employed. For example, aside from a propellant, use may be made of the above-mentioned solvents, preservatives, stabilizers, tonicity agents and pH adjustors. Propellants that may be used include liquefied gas propellants and compressed gas. Examples of liquefied gas propellants include fluorinated hydrocarbons (e.g., substitutes for CFC's, such as HCFC22, HCFC-123, HCFC-134a and HCFC142), liquefied petroleum and dimethyl ether. Illustrative examples of compressed gases include soluble gases (e.g., carbon dioxide, nitrous oxide) and insoluble gases (e.g., nitrogen gas).

[0068] The HGF protein used in the invention may be prepared as a sustained-release preparation (e.g., depot preparation) together with a biodegradable polymer. By preparing the HGF protein as a depot preparation in particular, a number of desirable effects can be expected, such as a decrease in the number of times it is administered, a good duration in the therapeutic effects, and the alleviation of side effects. Such sustained release preparations can be produced by a known method. The biodegradable polymer used in this sustained release preparation may be suitably selected from among known biodegradable polymers, such as polysaccharides, including starch, dextran, hyaluronan (hyaluronic acid) and salts thereof; proteins such as atelocollagen, collagen and gelatin; polyamino acids such as polyglutamic acid, polylysine, polyleucine, polyalanine and polymethionine; polylactic acid, polyglycolic acid, lactic acid-glycolic acid copolymers, polycaprolactone, poly-β-hydroxybutyricacid and polymalic acid; polyesters and polyorthoesters such as fumaric acid-polyethylene glycol-vinylpyrrolidone copolymers; polyalkylcyanoacrylic acids such as polymethyl-α-cyanoacrylic acid; and polycarbonates such as polyethylene carbonate and polypropylene carbonate. Polyesters are preferred, and lactic acid-glycolic acid copolymers are especially preferred. When lactic acid-glycolic acid copolymer is used, the compositional ratio (lactic acid/glycolic acid) thereof, in mol %, varies with the intended period of sustained release. For example, for a sustained release period of from about 2 weeks to about 3 months, and preferably from about 2 weeks to about 1 month, the ratio is in a range of from about 100/0 to about 50/50. The weight-average molecular weight of the lactic acid-glycolic acid copolymer is generally from about 5,000 to about 20,000. The lactic acid-glycolic acid copolymer may be produced by a known production method, such as that described in Japanese Patent Application Laid-open No. 61-28521. The compounding ratio of the biodegradable polymer and the HGF protein is not subject to any particular limitation. For example, the ratio of HGF protein to the biodegradable polymer is typically from about 0.01 to about 30 w/w %.

[0069] Administration is preferably carried out by, in the case of an injectable preparation or spray, direct injection (e.g., intrathecal administration, continuous intrathecal administration with a sustained-release pump) or spraying to the tissue affected by a spinal cord injury or demyelinating disease, or by, in the case of a sustained-release preparation (depot preparation), implantation of the preparation at a site near the tissue affected by a spinal cord injury or demyelinating disease. The dose is suitably selected according to such factors as the dosage form, severity of the disorder and the patient's age, and is generally from 1 μg to 500 mg, and preferably from 10 μg to 50 mg, per administration. The method of administration may also be suitably selected according to the dosage form, severity of the disorder, patient's age and other factors. For example, the therapeutic agent may be given as a single, one-time administration, as a single, sustained administration for a period ranging from about 30 minutes to several weeks (preferably for a period ranging from about 24 hours to about 2 weeks). Alternatively, the above one-time administration or sustained administration may be given repetitively at spaced intervals. In the case of repeated administration, the dosing interval may be from once a day to once every several months. For instance, in the case of administration as a sustained-release preparation (depot preparation) or in the case of local (e.g., intrathecal) sustained administration with a sustained-release pump (e.g., an osmotic pump), the dosing interval may be from several weeks to several months. Such sustained administration has the advantage that, since HGF protein is gradually released over an extended period of time at the site of spinal cord injury or demyelinating disease, the HGF acts over an extended period, enabling even better therapeutic effects to be achieved. A further advantage is that the less number of administrations eases the burden on the patient. A still further advantage is that, if necessary, additional HGF protein can be supplied to the subcutaneously implanted osmotic pump. As noted above, local administration is preferred as the method of administration, although other methods of administration such as intramuscular administration, subcutaneous administration or drip infusion are also possible. The period of administration is suitably selected according to such factors as the dosage forms, severity of the disorder and age of the patient. For example, in the case of a spinal cord injury, administration should take place preferably within 14 days, more preferably within 7 days, and most preferably within 4 days after the injury was sustained. In particular, in spinal cord injury patients, given how difficult it is to stabilize the condition of the patient within 72 hours following trauma, it is especially preferable for administration to take place from about 72 hours and within 4 days after the injury was sustained. The above period of administration includes the start of administration in the case of sustained administration, or the initial administration in the case of repetitive administration.

EXAMPLES

[0070] Examples are used below to describe the invention, although the invention is not limited by these examples. The HGF protein used in the following examples was a five-amino-acid-deleted-type recombinant HGF protein (SEQ ID NO: 2).

Example 1

[0071] (Preparation of Spinal Cord Injury Animals and Administration of HGF Protein)

[0072] (1) Preparation of Spinal Cord Injury Animals

[0073] First, an osmotic pump was sterilely prepared for use. HGF protein (concentration: 1 mg/mL; dissolved in PBS) or PBS (control) was poured into an Alzet miniosmotic pump (Model 2002, manufactured by ALZA Corporation). Silicone tube (catheter tube, manufactured by Imamura Co., Ltd.) having an inner diameter of 0.3 mm and an outer diameter of 0.7 mm whose lumen was filled with HGF protein or PBS was connected to the pump outlet and the junction was covered with another silicone tube (Imamura Co., Ltd.) having an inner diameter of 1.0 mm and an outer diameter of 2.0 mm, following which the pump and tube assembly was incubated at 37° C. for 12 hours, then furnished for use in the experiment.

[0074] Adult, female Sprague-Dawley rats (ranging in age from about 10 to 12 weeks, and having a body weight of about 250 g) were anesthetized by the intraperitoneal administration of 14 w/v % chloral hydrate, and the tenth and twelfth thoracic vertebrae were laminectomized. An osmotic pump (prefilled with HGF protein solution by the method described above) was then implanted subcutaneously on the right dorsal side, and the catheter tube was passed through the muscle layer from the subcutaneous area. Thereafter, the tip of the catheter tube was advanced to the position of the arch of the twelfth thoracic vertebra. Next, a 200 kDyne crushing injury was created at the tenth thoracic spinal cord segment using an IH impactor (manufactured by Precision Systems). Thereafter, the dura mater and arachnoid membrane of the twelfth thoracic spinal cord were split together in the direction of craniocaudal axis, from which the catheter tube was inserted into the subarachnoid space and the tip of the catheter was advanced to the point of the damaged spinal cord. The catheter was immobilized at inner and outer sides of the muscle layer with the surgical adhesive Aron Alpha A Sankyo (available from Sankyo Co., Ltd.). After the adhesive fully dried, the muscle layer and the skin were respectively sutured, completing the operation.

[0075] (2) Administration of HGF Protein

[0076] Following the operation (following the crushing injury), HGF protein solution was intrathecally administered for 2 weeks by means of the above-described osmotic pump (HGF protein dose, 200 μg/2 weeks). A control group was given only PBS.

Example 2

[0077] (Tissue Analysis and Results)

[0078] After a fixed postoperative period, the rats were deeply anesthetized by the intraperitoneal administration of 14 w/v % chloral hydrate, following which perfusion from the left ventricle of the heart was carried out, first with PBS, and then with 4 w/v % paraformaldehyde/PBS. A piece of the spinal cord was removed and subsequently fixed in 4 w/v % paraformaldehyde/PBS for 24 hours at 4° C. The tissue sample was immersed in a 10 w/v % sucrose/PBS solution and then a 30 w/v % sucrose/PBS solution each at 4° C. and for 24 hours, after which it was embedded in OCT compound (Sakura Fine Technical). The embedded tissue was immediately frozen in liquid nitrogen, and 20 μm frozen sections were prepared. The tissue sections were then stained with hematoxylin and eosin (HE), and histological examination was carried out. As a result, as shown in FIG. 1, cavity formation due to motor neutron degeneration and cell death was clearly suppressed in the HGF protein group as compared with the control group, thus indicating that spinal cord degeneration from crushing was suppressed.

Example 3

[0079] (Myelin Sheath Stain and Results)

[0080] The sections prepared by the method described in Example 2 were treated with 95 v/v % ethanol, following which they were incubated at 60° C. for 2 hours in a Luxor Fast Blue (LFB) solution. After removal from the incubator, the sections were left to stand to be cooled to room temperature and washed with 95 v/v % ethanol and distilled water. Next, fractionation with a lithium carbonate solution and 70 v/v % ethanol and washing with distilled water were repeatedly carried out until a suitable contrast was obtained, following which the sections were dehydrated and sealed, and the myelin sheath was examined. As shown in FIG. 2, the LFB-positive myelin sheath surface area in the HGF protein group was larger than that in the control group, indicating that demyelination due to spinal cord injury was suppressed.

Example 4

[0081] (Immunohistochemical Analysis and Results)

[0082] The sections prepared by the method described in Example 2 were stained with polyclonal 5HT antibody (1:100 dilution) and polyclonal anti-GAP43 antibody (1:1000 dilution). That is, one hour of blocking at room temperature was carried out with PBS containing 5 v/v % goat serum and 0.1 w/v % Triton X-100, following which the sections were incubated overnight in the antibody solution at 4° C. These sections were washed with PBS, then incubated at room temperature for one hour in a secondary antibody that was fluorescently-labeled with Alexa 488 (green) and Alexa 546 (red (1:1000 dilution), and sealed on slides, and 5HT positive nerve fibers and GAP43 positive nerve fibers were examined under a fluorescence microscope. As a result, as shown in FIG. 3, 5HT-positive nerve fibers were found to be significantly more abundant 4 mm caudal to the site of injury in the HGF protein group as compared with the control group. Moreover, as shown in FIG. 4, there was good agreement between the localizations of 5HT positive signals and GAP43 positive signals. The fact that the 5HT positive nerve fibers are responsible for motor function following spinal cord injury and that, in adults, GAP43 is expressed only in regenerated nerve fibers indicated that the regeneration of nerve fibers linked to motor function was facilitated by the administration of HGF protein.

Example 5

[0083] (Motor Function Evaluation and Results)

[0084] Motor function of animals treated with HGF protein (200 μg/2 weeks) starting immediately after spinal cord injury, by the method described in Example 1, was examined using an open field test. Behavior of animals was visually observed by a plurality of observers, and evaluated using the Basso-Beattie-Bresnahan (BBB) score system, in which motor function is evaluated on a 21-step scale ranging from 0 (completely paralyzed) to 21 (normal). Hindlimb motor function was evaluated for 6 weeks postoperatively. The results are shown in FIG. 5.

[0085] From FIG. 5, it is apparent that functional recovery was observed starting at 4 days following spinal cord injury in the HGF protein group, and significant functional recovery effects compared to the control group were observed from 5 weeks on (p<0.05).

Example 6

[0086] Spinal cord injury animals were prepared in the same way as in Example 1, following which an osmotic pump filled with HGF protein (2 mg/mL, dissolved in PBS) or PBS (control) was implanted and catheter insertion were carried out. Postoperatively (following crushing injury), an HGF protein solution was intrathecally administered (HGF protein dose, 400 μg/4 weeks) for a period of 4 weeks with the osmotic pump. The control group was given PBS only. Motor functional evaluation by the method described in Example 5 was carried out up until 9 weeks following the operation. The results are shown in FIG. 6.

[0087] As is apparent from FIG. 6, functional recovery in animals treated with HGF protein was significantly promoted compared with the control animals. The difference was evident from 4 days after spinal cord injury. In addition, a rise in the BBB score continued even after the completion of administration of HGF protein.

Example 7

[0088] Adult, female Sprague-Dawley rats (ranging in age from about 10 to 12 weeks, and having a body weight of about 250 g) were anesthetized by the intraperitoneal administration of 14 w/v % chloral hydrate, and a 200 kDyne crushing injury was created at the tenth thoracic spinal cord segment using an IH impactor (manufactured by Precision Systems), thereby giving a spinal cord injured animal. The spinal cord injured animal was reoperated at 4 days, 2 weeks or 8 weeks following the crushing injury in order to implant an osmotic pump. An osmotic pump filled with HGF protein (2 mg/mL, dissolved in PBS) or PBS (control) was subcutaneously implanted by the same method as in Example 1 and the catheter tube was inserted into the subarachnoid space, following which the tip of the catheter tube was advanced to the point of the damaged spinal cord and finally the catheter was immobilized. Starting 4 days, 2 weeks or 8 weeks after the crushing injury, the HGF protein solution was intrathecally administered from the osmotic pump over a period of 4 weeks (HGF protein dose: 400 μg/4 weeks). In the control group, PBS alone was administered. Motor function evaluation over time was carried out by the same method as in Example 5. The results are shown in FIGS. 7, 8 and 9.

[0089] In the animal group in which HGF treatment was started from 4 days after crushing injury, as is apparent from FIG. 7, faster functional recovery than in the control animals was observed.

[0090] In the animal group in which HGF treatment was started 2 weeks after the crushing injury, as is apparent from FIG. 8, the effect of promotion of functional recovery was observed 2 weeks after the start of HGF treatment (4 weeks after the crushing injury) in comparison with the control group.

[0091] In the animal group in which HGF treatment was started 8 weeks after the crushing injury, as is apparent from FIG. 9, no difference in functional recovery was observed between the animals given HGF protein and the control animals.

Preparation Example 1

[0092] A lactic acid-glycolic acid copolymer (1.9 g; lactic acid/glycolic acid=50/50; weight-average molecular weight=10,000; available from Wako Pure Chemical Industries, Ltd.) was dissolved in 3.0 mL of dichloromethane. Next, 100 mg of a freeze-dried powder of HGF protein was added to this organic solvent solution and finely milled using a mixer mill (Retsch Technology), thereby preparing an HGF protein dispersion. The dispersion was added to 800 mL of a 0.1 w/v % aqueous polyvinyl alcohol solution, then agitated and homogenized using a homomixer. The dichloromethane was evaporated off by 3 hours of stirring at room temperature, following which the microcapsules were collected by centrifugation at about 2,000 rpm. The microcapsules were then washed twice using 400 mL of distilled water, following which 0.2 g of D-mannitol was added and freeze-drying was carried out. To remove residual solvent, vacuum-drying was carried out for 3 days at 40° C., thereby giving HGF protein-containing sustained-release microcapsules (HGF proportion based on biopolymer: 5.3 w/w %).

Preparation Example 2

[0093] A lactic acid-glycolic acid copolymer (1.89 g; lactic acid/glycolic acid=50/50; weight-average molecular weight=10,000; available from Wako Pure Chemical Industries, Ltd.) and 10 mg of zinc oxide were dissolved in 3.0 mL of dichloromethane. Next, 100 mg of a freeze-dried powder of HGF protein was added to this organic solvent solution and finely milled using a mixer mill (Retsch Technology), thereby preparing an HGF protein dispersion. The dispersion was added to 800 mL of a 0.1 w/v % aqueous polyvinyl alcohol solution, then agitated and homogenized using a homomixer. The dichloromethane was evaporated off by 3 hours of stirring at room temperature, following which the microcapsules were collected by centrifugation at about 2,000 rpm. The microcapsules were then washed twice using 400 mL of distilled water, following which 0.2 g of D-mannitol was added and freeze-drying was carried out. To remove residual solvent, vacuum-drying was carried out for 3 days at 40° C., thereby giving HGF protein-containing sustained-release microcapsules (HGF proportion based on biopolymer: 5.3 w/w %).

Preparation Example 3

[0094] A lactic acid-glycolic acid copolymer (1.7 g; lactic acid/glycolic acid=75/25; weight-average molecular weight=15,000; available from Wako Pure Chemical Industries, Ltd.) was dissolved in 2.7 mL of dichloromethane. Next, 300 mg of a freeze-dried powder of HGF protein was added to this organic solvent solution and finely milled using a mixer mill (Retsch Technology), thereby preparing an HGF protein dispersion. The dispersion was added to 800 mL of a 0.1 w/v % aqueous polyvinyl alcohol solution, then agitated and homogenized using a homomixer. The dichloromethane was evaporated off by 3 hours of stirring at room temperature, following which the microcapsules were collected by centrifugation at about 2,000 rpm. The microcapsules were then washed twice using 400 mL of distilled water, following which 0.2 g of D-mannitol was added and freeze-drying was carried out. To remove residual solvent, vacuum-drying was carried out for 3 days at 40° C., thereby giving HGF protein-containing sustained-release microcapsules (HGF proportion based on biopolymer: 17.6 w/w %).

Preparation Example 4

[0095] A lactic acid-glycolic acid copolymer (1.69 g; lactic acid/glycolic acid=75/25; weight-average molecular weight=15,000; available from Wako Pure Chemical Industries, Ltd.) and 10 mg of zinc oxide were dissolved in 2.7 mL of dichloromethane. Next, 300 mg of a freeze-dried powder of HGF protein was added to this organic solvent solution and finely milled using a mixer mill (Retsch Technology), thereby preparing an HGF protein dispersion. The dispersion was added to 800 mL of a 0.1 w/v % aqueous polyvinyl alcohol solution, then agitated and homogenized using a homomixer. The dichloromethane was evaporated off by 3 hours of stirring at room temperature, following which the microcapsules were collected by centrifugation at about 2,000 rpm. The microcapsules were then washed twice using 400 mL of distilled water, following which 0.2 g of D-mannitol was added and freeze-drying was carried out. To remove residual solvent, vacuum-drying was carried out for 3 days at 40° C., thereby giving HGF protein-containing sustained-release microcapsules (HGF proportion based on biopolymer: 17.8 w/w %).

Preparation Example 5

[0096] A DL-lactic acid polymer (5 g; lactic acid/glycolic acid=100/0; weight-average molecular weight=5,000; available from Wako Pure Chemical Industries, Ltd.) was dissolved in 50 mL of methylene chloride, thereby preparing a 10 w/v % solution. Next, 2.5 mg of a freeze-dried powder of HGF protein was added to the solution. The resulting mixture was then added to a 0.5 w/v % aqueous solution of chitosan that had been separately warmed to 40° C., following which agitation and emulsification were carried out at a stirring speed of 1000 rpm using a homomixer. The resulting emulsion was stirred for another 3 hours at room temperature to evaporate off the methylene chloride, following which the microspheres that formed were collected by centrifugation at about 2,000 rpm. The microspheres were washed five times using distilled water that had been pre-warmed to 40° C., then vacuum-dried at room temperature, thereby giving HGF protein-containing microspheres (HGF proportion based on biopolymer: 0.05 w/w %).

Preparation Example 6

[0097] A lactic acid-glycolic acid copolymer (10 g; lactic acid/glycolic acid=75/25; weight-average molecular weight=5,000; available from Wako Pure Chemical Industries, Ltd.) was dissolved in 200 mL of methylene chloride/ethanol (4:1), thereby preparing a 5 w/v % solution. To this solution was added 2.5 mg of a freeze-dried powder of HGF protein. The resulting mixture was added a little at a time, under agitation with a homomixer at a speed of 500 rpm, to a 1 w/v % aqueous gelatin solution that was separately warmed to 40° C., thereby effecting emulsification. The resulting emulsion was additionally stirred at room temperature for 3 hours to evaporate off the methylene chloride and ethanol, following which the microspheres that formed were collected by centrifugation at about 2,000 rpm, washed five times with distilled water that had been pre-warmed to 40° C., and vacuum-dried at room temperature, thereby giving HGF protein-containing microspheres (HGF proportion based on biopolymer: 0.025 w/w %).

Preparation Example 7

[0098] A 2 w/v % aqueous solution of HGF protein (0.2 mL) was mixed with 2 mL of a 2% phosphate buffer solution of atelocollagen, and freeze-dried. The freeze-dried material was fractured at low temperature using liquid nitrogen, then placed in a mold and compression-molded to form a cylindrical HGF-containing sustained-released preparation (HGF proportion based on biopolymer: 10 w/w %).

Preparation Example 8

[0099] A 0.01 w/v % aqueous solution of HGF protein (100 mL) and 50 g of a 2 w/v % aqueous solution of collagen were uniformly mixed together and agitated, and then freeze-dried. The freeze-dried material was low-temperature fractured using liquid nitrogen, following which the fractured material was compression molded into a stick, thereby giving an HGF-containing sustained-released preparation (HGF proportion based on biopolymer: 1 w/w %).

Preparation Example 9

[0100] HGF protein (1 mg) was dissolved in 2 ml of a 2 w/v % atelocollagen solution, following which freeze drying was carried out. The resulting composite was fractured, and then compression-molded into a cylindrical shape, giving an HGF-containing sustained-release preparation (HGF proportion based on biopolymer, 2.5 wt %).

Preparation Example 10

[0101] The sodium salt of hyaluronan (0.58 g; intrinsic viscosity, 4500 cc/g) was mixed with 20 mL of water and made to swell. Next, 2 mL of 2N sodium hydroxide was added to this mixture, and stirring was carried out to give a uniform solution. Divinylsulfone (0.10 g) in 2.4 mL of water was then added and stirred. The resulting mixture was left to stand for 70 minutes, after which the gel that formed was placed in 223 mL of a Biotris buffer solution (a phosphate buffer containing 0.15 M of NaCl and having a pH of about 7.2), and swelling was induced for 3 hours. Next, 1 mL of 2N HCl was added to this mixture. After 1 hour, 0.6 mL of 2 N HCl was added, and the mixture was left to stand for 16 hours. This was followed by the addition of 0.35 mL of 2N HCl, after which the swelled gel was slowly stirred in the buffer solution for 3 days. A soft gel having uniform viscoelastic properties was obtained. The gel was dialyzed with 0.15 M NaCl for 5 days. The gel was then mixed with 1 w/v % HGF protein in buffered saline so as to set the final concentration of HGF protein to 0.25 w/v %, thereby giving an HGF-containing preparation (HGF proportion based on biopolymer: 25 w/v %).

INDUSTRIAL APPLICABILITY

[0102] The present invention provides an agent useful for treating spinal cord injuries and demyelinating diseases.

Sequence CWU 1

31728PRTHomo sapiens 1Met Trp Val Thr Lys Leu Leu Pro Ala Leu Leu Leu Gln His Val Leu1 5 10 15Leu His Leu Leu Leu Leu Pro Ile Ala Ile Pro Tyr Ala Glu Gly Gln 20 25 30Arg Lys Arg Arg Asn Thr Ile His Glu Phe Lys Lys Ser Ala Lys Thr 35 40 45Thr Leu Ile Lys Ile Asp Pro Ala Leu Lys Ile Lys Thr Lys Lys Val 50 55 60Asn Thr Ala Asp Gln Cys Ala Asn Arg Cys Thr Arg Asn Lys Gly Leu65 70 75 80Pro Phe Thr Cys Lys Ala Phe Val Phe Asp Lys Ala Arg Lys Gln Cys 85 90 95Leu Trp Phe Pro Phe Asn Ser Met Ser Ser Gly Val Lys Lys Glu Phe 100 105 110Gly His Glu Phe Asp Leu Tyr Glu Asn Lys Asp Tyr Ile Arg Asn Cys 115 120 125Ile Ile Gly Lys Gly Arg Ser Tyr Lys Gly Thr Val Ser Ile Thr Lys 130 135 140Ser Gly Ile Lys Cys Gln Pro Trp Ser Ser Met Ile Pro His Glu His145 150 155 160Ser Phe Leu Pro Ser Ser Tyr Arg Gly Lys Asp Leu Gln Glu Asn Tyr 165 170 175Cys Arg Asn Pro Arg Gly Glu Glu Gly Gly Pro Trp Cys Phe Thr Ser 180 185 190Asn Pro Glu Val Arg Tyr Glu Val Cys Asp Ile Pro Gln Cys Ser Glu 195 200 205Val Glu Cys Met Thr Cys Asn Gly Glu Ser Tyr Arg Gly Leu Met Asp 210 215 220His Thr Glu Ser Gly Lys Ile Cys Gln Arg Trp Asp His Gln Thr Pro225 230 235 240His Arg His Lys Phe Leu Pro Glu Arg Tyr Pro Asp Lys Gly Phe Asp 245 250 255Asp Asn Tyr Cys Arg Asn Pro Asp Gly Gln Pro Arg Pro Trp Cys Tyr 260 265 270Thr Leu Asp Pro His Thr Arg Trp Glu Tyr Cys Ala Ile Lys Thr Cys 275 280 285Ala Asp Asn Thr Met Asn Asp Thr Asp Val Pro Leu Glu Thr Thr Glu 290 295 300Cys Ile Gln Gly Gln Gly Glu Gly Tyr Arg Gly Thr Val Asn Thr Ile305 310 315 320Trp Asn Gly Ile Pro Cys Gln Arg Trp Asp Ser Gln Tyr Pro His Glu 325 330 335His Asp Met Thr Pro Glu Asn Phe Lys Cys Lys Asp Leu Arg Glu Asn 340 345 350Tyr Cys Arg Asn Pro Asp Gly Ser Glu Ser Pro Trp Cys Phe Thr Thr 355 360 365Asp Pro Asn Ile Arg Val Gly Tyr Cys Ser Gln Ile Pro Asn Cys Asp 370 375 380Met Ser His Gly Gln Asp Cys Tyr Arg Gly Asn Gly Lys Asn Tyr Met385 390 395 400Gly Asn Leu Ser Gln Thr Arg Ser Gly Leu Thr Cys Ser Met Trp Asp 405 410 415Lys Asn Met Glu Asp Leu His Arg His Ile Phe Trp Glu Pro Asp Ala 420 425 430Ser Lys Leu Asn Glu Asn Tyr Cys Arg Asn Pro Asp Asp Asp Ala His 435 440 445Gly Pro Trp Cys Tyr Thr Gly Asn Pro Leu Ile Pro Trp Asp Tyr Cys 450 455 460Pro Ile Ser Arg Cys Glu Gly Asp Thr Thr Pro Thr Ile Val Asn Leu465 470 475 480Asp His Pro Val Ile Ser Cys Ala Lys Thr Lys Gln Leu Arg Val Val 485 490 495Asn Gly Ile Pro Thr Arg Thr Asn Ile Gly Trp Met Val Ser Leu Arg 500 505 510Tyr Arg Asn Lys His Ile Cys Gly Gly Ser Leu Ile Lys Glu Ser Trp 515 520 525Val Leu Thr Ala Arg Gln Cys Phe Pro Ser Arg Asp Leu Lys Asp Tyr 530 535 540Glu Ala Trp Leu Gly Ile His Asp Val His Gly Arg Gly Asp Glu Lys545 550 555 560Cys Lys Gln Val Leu Asn Val Ser Gln Leu Val Tyr Gly Pro Glu Gly 565 570 575Ser Asp Leu Val Leu Met Lys Leu Ala Arg Pro Ala Val Leu Asp Asp 580 585 590Phe Val Ser Thr Ile Asp Leu Pro Asn Tyr Gly Cys Thr Ile Pro Glu 595 600 605Lys Thr Ser Cys Ser Val Tyr Gly Trp Gly Tyr Thr Gly Leu Ile Asn 610 615 620Tyr Asp Gly Leu Leu Arg Val Ala His Leu Tyr Ile Met Gly Asn Glu625 630 635 640Lys Cys Ser Gln His His Arg Gly Lys Val Thr Leu Asn Glu Ser Glu 645 650 655Ile Cys Ala Gly Ala Glu Lys Ile Gly Ser Gly Pro Cys Glu Gly Asp 660 665 670Tyr Gly Gly Pro Leu Val Cys Glu Gln His Lys Met Arg Met Val Leu 675 680 685Gly Val Ile Val Pro Gly Arg Gly Cys Ala Ile Pro Asn Arg Pro Gly 690 695 700Ile Phe Val Arg Val Ala Tyr Tyr Ala Lys Trp Ile His Lys Ile Ile705 710 715 720Leu Thr Tyr Lys Val Pro Gln Ser 7252723PRTHomo sapiens 2Met Trp Val Thr Lys Leu Leu Pro Ala Leu Leu Leu Gln His Val Leu1 5 10 15Leu His Leu Leu Leu Leu Pro Ile Ala Ile Pro Tyr Ala Glu Gly Gln 20 25 30Arg Lys Arg Arg Asn Thr Ile His Glu Phe Lys Lys Ser Ala Lys Thr 35 40 45Thr Leu Ile Lys Ile Asp Pro Ala Leu Lys Ile Lys Thr Lys Lys Val 50 55 60Asn Thr Ala Asp Gln Cys Ala Asn Arg Cys Thr Arg Asn Lys Gly Leu65 70 75 80Pro Phe Thr Cys Lys Ala Phe Val Phe Asp Lys Ala Arg Lys Gln Cys 85 90 95Leu Trp Phe Pro Phe Asn Ser Met Ser Ser Gly Val Lys Lys Glu Phe 100 105 110Gly His Glu Phe Asp Leu Tyr Glu Asn Lys Asp Tyr Ile Arg Asn Cys 115 120 125Ile Ile Gly Lys Gly Arg Ser Tyr Lys Gly Thr Val Ser Ile Thr Lys 130 135 140Ser Gly Ile Lys Cys Gln Pro Trp Ser Ser Met Ile Pro His Glu His145 150 155 160Ser Tyr Arg Gly Lys Asp Leu Gln Glu Asn Tyr Cys Arg Asn Pro Arg 165 170 175Gly Glu Glu Gly Gly Pro Trp Cys Phe Thr Ser Asn Pro Glu Val Arg 180 185 190Tyr Glu Val Cys Asp Ile Pro Gln Cys Ser Glu Val Glu Cys Met Thr 195 200 205Cys Asn Gly Glu Ser Tyr Arg Gly Leu Met Asp His Thr Glu Ser Gly 210 215 220Lys Ile Cys Gln Arg Trp Asp His Gln Thr Pro His Arg His Lys Phe225 230 235 240Leu Pro Glu Arg Tyr Pro Asp Lys Gly Phe Asp Asp Asn Tyr Cys Arg 245 250 255Asn Pro Asp Gly Gln Pro Arg Pro Trp Cys Tyr Thr Leu Asp Pro His 260 265 270Thr Arg Trp Glu Tyr Cys Ala Ile Lys Thr Cys Ala Asp Asn Thr Met 275 280 285Asn Asp Thr Asp Val Pro Leu Glu Thr Thr Glu Cys Ile Gln Gly Gln 290 295 300Gly Glu Gly Tyr Arg Gly Thr Val Asn Thr Ile Trp Asn Gly Ile Pro305 310 315 320Cys Gln Arg Trp Asp Ser Gln Tyr Pro His Glu His Asp Met Thr Pro 325 330 335Glu Asn Phe Lys Cys Lys Asp Leu Arg Glu Asn Tyr Cys Arg Asn Pro 340 345 350Asp Gly Ser Glu Ser Pro Trp Cys Phe Thr Thr Asp Pro Asn Ile Arg 355 360 365Val Gly Tyr Cys Ser Gln Ile Pro Asn Cys Asp Met Ser His Gly Gln 370 375 380Asp Cys Tyr Arg Gly Asn Gly Lys Asn Tyr Met Gly Asn Leu Ser Gln385 390 395 400Thr Arg Ser Gly Leu Thr Cys Ser Met Trp Asp Lys Asn Met Glu Asp 405 410 415Leu His Arg His Ile Phe Trp Glu Pro Asp Ala Ser Lys Leu Asn Glu 420 425 430Asn Tyr Cys Arg Asn Pro Asp Asp Asp Ala His Gly Pro Trp Cys Tyr 435 440 445Thr Gly Asn Pro Leu Ile Pro Trp Asp Tyr Cys Pro Ile Ser Arg Cys 450 455 460Glu Gly Asp Thr Thr Pro Thr Ile Val Asn Leu Asp His Pro Val Ile465 470 475 480Ser Cys Ala Lys Thr Lys Gln Leu Arg Val Val Asn Gly Ile Pro Thr 485 490 495Arg Thr Asn Ile Gly Trp Met Val Ser Leu Arg Tyr Arg Asn Lys His 500 505 510Ile Cys Gly Gly Ser Leu Ile Lys Glu Ser Trp Val Leu Thr Ala Arg 515 520 525Gln Cys Phe Pro Ser Arg Asp Leu Lys Asp Tyr Glu Ala Trp Leu Gly 530 535 540Ile His Asp Val His Gly Arg Gly Asp Glu Lys Cys Lys Gln Val Leu545 550 555 560Asn Val Ser Gln Leu Val Tyr Gly Pro Glu Gly Ser Asp Leu Val Leu 565 570 575Met Lys Leu Ala Arg Pro Ala Val Leu Asp Asp Phe Val Ser Thr Ile 580 585 590Asp Leu Pro Asn Tyr Gly Cys Thr Ile Pro Glu Lys Thr Ser Cys Ser 595 600 605Val Tyr Gly Trp Gly Tyr Thr Gly Leu Ile Asn Tyr Asp Gly Leu Leu 610 615 620Arg Val Ala His Leu Tyr Ile Met Gly Asn Glu Lys Cys Ser Gln His625 630 635 640His Arg Gly Lys Val Thr Leu Asn Glu Ser Glu Ile Cys Ala Gly Ala 645 650 655Glu Lys Ile Gly Ser Gly Pro Cys Glu Gly Asp Tyr Gly Gly Pro Leu 660 665 670Val Cys Glu Gln His Lys Met Arg Met Val Leu Gly Val Ile Val Pro 675 680 685Gly Arg Gly Cys Ala Ile Pro Asn Arg Pro Gly Ile Phe Val Arg Val 690 695 700Ala Tyr Tyr Ala Lys Trp Ile His Lys Ile Ile Leu Thr Tyr Lys Val705 710 715 720Pro Gln Ser3728PRTRattus norvegicus 3Met Met Trp Gly Thr Lys Leu Leu Pro Val Leu Leu Leu Gln His Val1 5 10 15Leu Leu His Leu Leu Leu Leu Pro Val Thr Ile Pro Tyr Ala Glu Gly 20 25 30Gln Lys Lys Arg Arg Asn Thr Leu His Glu Phe Lys Lys Ser Ala Lys 35 40 45Thr Thr Leu Thr Lys Glu Asp Pro Leu Val Lys Ile Lys Thr Lys Lys 50 55 60Val Asn Ser Ala Asp Glu Cys Ala Asn Arg Cys Ile Arg Asn Lys Gly65 70 75 80Phe Pro Phe Thr Cys Lys Ala Phe Val Phe Asp Lys Ser Arg Lys Arg 85 90 95Cys Tyr Trp Tyr Pro Phe Asn Ser Met Ser Ser Gly Val Lys Lys Gly 100 105 110Phe Gly His Glu Phe Asp Leu Tyr Glu Asn Lys Asp Tyr Ile Arg Asn 115 120 125Cys Ile Ile Gly Lys Gly Gly Ser Tyr Lys Gly Thr Val Ser Ile Thr 130 135 140Lys Ser Gly Ile Lys Cys Gln Pro Trp Asn Ser Met Ile Pro His Glu145 150 155 160His Ser Phe Leu Pro Ser Ser Tyr Arg Gly Lys Asp Leu Gln Glu Asn 165 170 175Tyr Cys Arg Asn Pro Arg Gly Glu Glu Gly Gly Pro Trp Cys Phe Thr 180 185 190Ser Asn Pro Glu Val Arg Tyr Glu Val Cys Asp Ile Pro Gln Cys Ser 195 200 205Glu Val Glu Cys Met Thr Cys Asn Gly Glu Ser Tyr Arg Gly Pro Met 210 215 220Asp His Thr Glu Ser Gly Lys Thr Cys Gln Arg Trp Asp Gln Gln Thr225 230 235 240Pro His Arg His Lys Phe Leu Pro Glu Arg Tyr Pro Asp Lys Gly Phe 245 250 255Asp Asp Asn Tyr Cys Arg Asn Pro Asp Gly Lys Pro Arg Pro Trp Cys 260 265 270Tyr Thr Leu Asp Pro Asp Thr Pro Trp Glu Tyr Cys Ala Ile Lys Met 275 280 285Cys Ala His Ser Ala Val Asn Glu Thr Asp Val Pro Met Glu Thr Thr 290 295 300Glu Cys Ile Lys Gly Gln Gly Glu Gly Tyr Arg Gly Thr Thr Asn Thr305 310 315 320Ile Trp Asn Gly Ile Pro Cys Gln Arg Trp Asp Ser Gln Tyr Pro His 325 330 335Lys His Asp Ile Thr Pro Glu Asn Phe Lys Cys Lys Asp Leu Arg Glu 340 345 350Asn Tyr Cys Arg Asn Pro Asp Gly Ala Glu Ser Pro Trp Cys Phe Thr 355 360 365Thr Asp Pro Asn Ile Arg Val Gly Tyr Cys Ser Gln Ile Pro Lys Cys 370 375 380Asp Val Ser Ser Gly Gln Asp Cys Tyr Arg Gly Asn Gly Lys Asn Tyr385 390 395 400Met Gly Asn Leu Ser Lys Thr Arg Ser Gly Leu Thr Cys Ser Met Trp 405 410 415Asp Lys Asn Met Glu Asp Leu His Arg His Ile Phe Trp Glu Pro Asp 420 425 430Ala Ser Lys Leu Thr Lys Asn Tyr Cys Arg Asn Pro Asp Asp Asp Ala 435 440 445His Gly Pro Trp Cys Tyr Thr Gly Asn Pro Leu Val Pro Trp Asp Tyr 450 455 460Cys Pro Ile Ser Arg Cys Glu Gly Asp Thr Thr Pro Thr Ile Val Asn465 470 475 480Leu Asp His Pro Val Ile Ser Cys Ala Lys Thr Lys Gln Leu Arg Val 485 490 495Val Asn Gly Ile Pro Thr Gln Thr Thr Val Gly Trp Met Val Ser Leu 500 505 510Lys Tyr Arg Asn Lys His Ile Cys Gly Gly Ser Leu Ile Lys Glu Ser 515 520 525Trp Val Leu Thr Ala Arg Gln Cys Phe Pro Ala Arg Asn Lys Asp Leu 530 535 540Lys Asp Tyr Glu Ala Trp Leu Gly Ile His Asp Val His Glu Arg Gly545 550 555 560Glu Glu Lys Arg Lys Gln Ile Leu Asn Ile Ser Gln Leu Val Tyr Gly 565 570 575Pro Glu Gly Ser Asp Leu Val Leu Leu Lys Leu Ala Arg Pro Ala Ile 580 585 590Leu Asp Asn Phe Val Ser Thr Ile Asp Leu Pro Ser Tyr Gly Cys Thr 595 600 605Ile Pro Glu Lys Thr Thr Cys Ser Ile Tyr Gly Trp Gly Tyr Thr Gly 610 615 620Leu Ile Asn Ala Asp Gly Leu Leu Arg Val Ala His Leu Tyr Ile Met625 630 635 640Gly Asn Glu Lys Cys Ser Gln His His Gln Gly Lys Val Thr Leu Asn 645 650 655Glu Ser Glu Leu Cys Ala Gly Ala Glu Lys Ile Gly Ser Gly Pro Cys 660 665 670Glu Gly Asp Tyr Gly Gly Pro Leu Ile Cys Glu Gln His Lys Met Arg 675 680 685Met Val Leu Gly Val Ile Val Pro Gly Arg Gly Cys Ala Ile Pro Asn 690 695 700Arg Pro Gly Ile Phe Val Arg Val Ala Tyr Tyr Ala Lys Trp Ile His705 710 715 720Lys Val Ile Leu Thr Tyr Lys Leu 725

Claims

1. A sustained release hepatocyte growth factor (HGF) preparation comprising: an HGF protein and a biopolymer, wherein said biopolymer is a lactic acid-glycolic acid copolymer having a weight average molecular weight from about 5,000 to about 20,000 Daltons and wherein the proportion of HGF to the biopolymer is from about 0.01 to about 30 w/w %.

2. The sustained release hepatocyte growth factor (HGF) preparation according to claim 1, wherein the HGF protein has the amino acid sequence of SEQ ID NO: 1 or 2.

3. The sustained release hepatocyte growth factor (HGF) preparation according to claim 1, wherein the HGF protein has the amino acid sequence of SEQ ID NO: 2.

4. The sustained release hepatocyte growth factor (HGF) preparation according to claim 1, wherein the preparation is formulated for local administration at a site of spinal cord injury.

5. The sustained release hepatocyte growth factor (HGF) preparation according to claim 3, wherein the preparation is an injectable form suitable for intrathecal administration.

6. The sustained release hepatocyte growth factor (HGF) preparation according to claim 5, wherein the preparation is suitable for intrathecal administration by a sustained-release pump.

7. A sustained release hepatocyte growth factor (HGF) preparation comprising: an HGF protein and a biopolymer, wherein said biopolymer is a biodegradable polymer selected from the group consisting of a lactic acid polymer, lactic acid-glycolic acid copolymer, atelocollagen, collagen, and hyluronan.

8. The sustained release hepatocyte growth factor (HGF) preparation according to claim 7, wherein the HGF protein has the amino acid sequence of SEQ ID NO: 2.

9. The sustained release hepatocyte growth factor (HGF) preparation according to claim 7, wherein the preparation is formulated for local administration at a site of spinal cord injury.

10. The sustained release hepatocyte growth factor (HGF) preparation according to claim 7, wherein the preparation is an injectable form suitable for intrathecal administration.

11. The sustained release hepatocyte growth factor (HGF) preparation according to claim 7, wherein the preparation is suitable for intrathecal administration by a sustained-release pump.

12. A method of improving motor function in an animal having a spinal cord injury comprising: selecting an animal having a spinal cord injury; providing said animal a hepatocyte growth factor (HGF) preparation at the site of said spinal cord injury; and evaluating the motor function of said animal.

13. The method of claim 12, wherein said hepatocyte growth factor (HGF) preparation is provided within 2 weeks following said spinal cord injury.

14. The method of claim 12, wherein said hepatocyte growth factor (HGF) preparation is provided within 2 weeks following said spinal cord injury.

15. The method of claim 12, wherein the hepatocyte growth factor (HGF) preparation comprises an HGF protein has the amino acid sequence of SEQ ID NO: 2.

16. The method of claim 12, wherein the hepatocyte growth factor (HGF) preparation is formulated for local administration at a site of spinal cord injury.

17. The method of claim 12, wherein the hepatocyte growth factor (HGF) preparation is an injectable form suitable for intrathecal administration.

18. The method of claim 17, wherein the hepatocyte growth factor (HGF) preparation is suitable for intrathecal administration by a sustained-release pump.

19. The method of claim 12, wherein said hepatocyte growth factor (HGF) preparation of claim 1 is provided.

20. The method of claim 12, wherein said hepatocyte growth factor (HGF) preparation of claim 7 is provided.

21. A method of suppressing demyelination in an animal having a spinal cord injury comprising: selecting an animal having a spinal cord injury; providing said animal a hepatocyte growth factor (HGF) preparation at the site of said spinal cord injury; and evaluating the demyelination of said animal.

22. The method of claim 21, wherein said hepatocyte growth factor (HGF) preparation is provided within 2 weeks following said spinal cord injury.

23. The method of claim 21, wherein said hepatocyte growth factor (HGF) preparation is provided within 2 weeks following said spinal cord injury.

24. The method of claim 21, wherein the hepatocyte growth factor (HGF) preparation comprises an HGF protein has the amino acid sequence of SEQ ID NO: 2.

25. The method of claim 21, wherein the hepatocyte growth factor (HGF) preparation is formulated for local administration at a site of spinal cord injury.

26. The method of claim 21, wherein the hepatocyte growth factor (HGF) preparation is an injectable form suitable for intrathecal administration.

27. The method of claim 26, wherein the hepatocyte growth factor (HGF) preparation is suitable for intrathecal administration by a sustained-release pump.

28. The method of claim 21, wherein said hepatocyte growth factor (HGF) preparation of claim 1 is provided.

29. The method of claim 21, wherein said hepatocyte growth factor (HGF) preparation of claim 7 is provided.

30. The method of claim 21, wherein the animal having a spinal cord injury has a disease selected from the group consisting of multiple sclerosis, Devic disease, Balo's concentric sclerosis, acute disseminated encephalomyelitis (ADEM), Schilder disease, subacute sclerosing panencephalitis (SSPE), progressive multifocal leukoencephalopathy (PML), Binswanger disease, hypoxic encephalopathy, central pontine myelinolysis, Guillain-Barre syndrome, Fischer syndrome, and chronic inflammatory demyelinating polyradiculoneuropathy (CIDP).