ERBB3 BASED METHODS AND COMPOSITIONS FOR TREATING NEOPLASMS

Abstract

The present invention relates to compositions and methods for treating neoplasms in mammals, particularly humans. More particularly, the present invention provides for methods for preventing, treating or delaying neoplasm in a mammal using an ErbB-3 protein, a nucleic acid encoding an ErbB-3 protein or a functional fragment thereof. The present invention also provides for an isolated nucleic acids encoding an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, substantially purified extracellular domain of the ErbB-3 protein, or a functional fragment thereof and antibodies that bind to an epitope in an extracellular domain of the ErbB-3 protein, or a functional fragment thereof. The present invention further provides for pharmaceutical compositions and/or vaccines comprising the extracellular domain of the ErbB-3 protein, or a functional fragment thereof, or nucleic acids encoding and antibodies binding to such extracellular domain or functional fragments thereof.

Description

[0001] This application is a divisional application of U.S. application Ser. No. 10/516,759, filed on Mar. 3, 2006, which is a national phase of PCT/CN03/00217, having an international filing date of Mar. 26, 2003, which claims priority from the Chinese Patent Application No. 02116259.x, filed Mar. 26, 2002, now Chinese Patent No. 03806762.5. The disclosures of the above applications are incorporated herein by reference in their entirety.

TECHNICAL FIELD

[0002] The present invention relates to compositions and methods for treating neoplasms in mammals, particularly humans. More particularly, the present invention provides for methods for preventing, treating or delaying neoplasm in a mammal using an ErbB-3 protein, a nucleic acid encoding an ErbB-3 protein or a functional fragment thereof. The present invention also provides for isolated nucleic acids encoding an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, substantially purified extracellular domain of the ErbB-3 protein, or a functional fragment thereof and antibodies that bind to an epitope in an extracellular domain of the ErbB-3 protein, or a functional fragment thereof. The present invention further provides for pharmaceutical compositions and/or vaccines comprising the extracellular domain of the ErbB-3 protein, or a functional fragment thereof, or nucleic acids encoding and antibodies binding to such extracellular domain or functional fragments thereof.

BACKGROUND ART

[0003] Cancer is a major lethal disease for humans and is caused by physiologically-uncontrolled cell proliferation which affects normal physiological conditions of human body resulting in serious pathological reactions often leading to death. Although tremendous efforts on cancer studies and treatments have been made, presently, cancer is still the major cause of death to humans. There are multiple approaches to treat cancer patients including surgery, radiation therapy and chemotherapy. As the first two methods are not able to completely eliminate cancer cells in patients, the latter approach is commonly used to control cancer cell growth with or without other treatments. Anti-cancer compounds used in patients are often targeting prevention of cancer cell proliferation or killing dividing cells.

When the compounds are toxic to cancer cells, they may also severely affect normal dividing cells which are necessary for human life. Therefore, one of main directions in cancer studies is to find methods to specifically block or kill cancer cells without affecting normal cell proliferation. There is a demand now for such treatment on cancer patients.

[0004] ErbBs are class one receptor protein tyrosine kinases. ErbB-mediated cell signaling plays a critical role in embryo development and adult organ function. On a cellular level, ErbB receptors have been shown to mediate signals for cell proliferation, differentiation, migration, and cell structure reorganization. There are four structurally similar ErbB members, ErbB-1, ErbB-2, ErbB-3 and ErbB-4. The epidermal growth factor (EGF) is one of several ligands that bind ErbB-1, ErbB-3 or ErbB-4 also bind several ligands, including neuregulin-1 (NRG-1). To date, no ligand for ErbB-2 has been identified. However, ErbB-2 serves as a heterodimer partner for ErbB-3, ErbB-4 or ErbB-1 and is critically involved in NRG-1-activated cell signaling. In vivo studies using gene targeting experiments indicate that developmental defects resulting from inactivation of ErbB-2 are similar to those observed in NRG-1-inactivated animals. Both animals show defects in the neural crania ganglia and heart trabeculae development. Furthermore, ErbB-3 or ErbB-4 gene-inactivated mice have similar or overlapping phenotypes to NRG-1 or ErbB-2 knockout mice.

[0005] In addition to its role in development, the human ErbB-2 gene is frequently amplified and its encoded protein is over-expressed in a variety of human carcinomas. Early research on ErbB-2 discovered that an oncogenic point mutation resulted in the formation of ErbB-2 homodimers that in turn caused significant phosphorylation of the tyrosine residues on the intracellular domain. While no corresponding point mutation has been found in ErbB-2 over expressing human carcinomas, the upregulation of ErbB-2 results in the formation of homodimers that in turn increases the tyrosine phosphorylation of its intracellular domain. This process is hypothesized to be the start of a signal cascade that triggers cell transformation and/or growth, and thus initiate tumorigenesis. There is evidence, however, to contradict the hypothesis that ErbB-2 homodimers are responsible for the initiation of tumorigenesis: i) some ErbB-2 mutants that are engineered to enhanced dimerization and self-phosphorylation have no effect on cell transformation; ii) antibodies that bind to the extracellular domain of ErbB-2 and presumably promote homodimerization result in ErbB2-expressing cancer cell growth promotion, whereas others inhibit cancer cell growth. These data indicate that homodimerization of ErbB-2 is insufficient for cell growth promotion or cell transformation, and other conditions, possibly involving specific dimer orientation or conformation, are required.

[0006] ErbB-2 acts as a heterodimer partner for the ligand-binding ErbB-3 or ErbB-4 receptors. The ligand, NRG-1, has been identified to have two independent receptor binding sites: one that has a high affinity for ErbB-3 or ErbB-4, and the other that has a low but non-specific affinity for all ErbB members. Thus, the exposure of NRG-1 to cells expressing ErbB-3/4 and ErbB-2 would result in heterodimers of ErbB-2 and ErbB-3/4. In the absence of the ligand, however, it is unclear whether ErbB-2 has an affinity with other ErbB receptors, and it is possible that such an interaction could be involved in the initiation of cancer. Amongst all the ErbB receptors, ErbB-3 is unique because: i) ErbB-2 preferentially forms heterodimers with ErbB-3; ii) cotransfection of NIH3T3 cells with ErbB-2 and ErbB-3 results in much higher levels of cell transformation than that of transfection with ErbB-2 alone; iii) in ErbB-2 over-expression-associated breast cancer cells, ErbB-3 is also highly expressed; and iv) ErbB-3 is also over expressed in ErbB-2-over expressing tumour cells from ErbB-2 transgenic mice.

[0007] A number of patents and patent applications disclose ErbB-2 and/or ErbB-3 related neoplasm or cancer treatment. For example, WO 00/78347 discloses methods for arresting or inhibiting cell growth, particularly cancer cell growth, comprising preventing or reducing ErbB-2/ErbB-3 heterodimer formation, or interfering with ErbB-2/ErbB-3 heterodimer conformation in a cell and agents which prevent or reduce ErbB-2/ErbB-3 heterodimer formation or interfere with ErbB-2/ErbB-3 heterodimer conformation in a cell thereby arresting or inhibiting the growth of the cell. U.S. Pat. No. 5,578,482 relates to erbB-2 ligands and functional derivatives thereof which are capable of binding to the erbB-2 oncogene product. U.S. Pat. No. 5,820,859 relates to a method of targeting a therapeutic agent to cells expressing the erb B-3 receptor. U.S. Pat. No. 5,968,511 relates to ErbB3 antibodies.

[0008] There exists a need in the art for more efficient and/or cost effective ErbB-3 related neoplasm treatments. The present invention addresses this and other related needs in the art.

DISCLOSURE OF THE INVENTION

[0009] In one aspect, the present invention is directed to a method for preventing, treating or delaying neoplasm in a mammal, which method comprises administering to a mammal, to which such prevention, treatment or delay is needed or desirable, an effective amount of an ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, whereby an immune response is generated against said neoplasm and said neoplasm is prevented, treated or delayed.

[0010] In another aspect, the present invention is directed to an isolated nucleic acid fragment, which isolated nucleic acid fragment comprises a sequence of nucleotides encoding an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 (FIG. 5) or SEQ ID NO:3 (FIG. 11) or an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14 or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16.

[0011] In still another aspect, the present invention is directed to a substantially purified protein or peptide, which comprises an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 or an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14; or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16.

[0012] In yet another aspect, the present invention is directed to a conjugate, which conjugate comprises: a) a protein or peptide comprising an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 or an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14; or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16.; and b) a facilitating agent linked to the extracellular domain of the ErbB-3 protein, or a functional fragment thereof, directly or via a linker, wherein the agent facilitates: i) affinity isolation or purification of a conjugate; ii) attachment of a conjugate to a surface; or iii) detection of a conjugate.

[0013] In yet another aspect, the present invention is directed to an antibody, which antibody binds to an epitope in an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 or an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14; or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16.

[0014] Pharmaceutical compositions and/or vaccines comprising the extracellular domain of the ErbB-3 protein, or a functional fragment thereof, or nucleic acids encoding and antibodies binding to such extracellular domain or functional fragments thereof are also provided.

BRIEF DESCRIPTION OF THE DRAWINGS

[0015] FIG. 1 depicts B3 cDNA sequence (SEQ ID NO:4).

[0016] FIG. 2 illustrates restriction enzyme digestion of B3 plasmid. Lane 1:1 KB ladder (NEB). Lane 2-9: DNA for diagnostic digestion with BamHI/XbaI. All are correct clones except the colony on Lane 5. Lane 10: pCDNA3 vector alone digested with BamHI/XbaI.

[0017] FIG. 3 illustrates B3 plasmid construction.

[0018] FIG. 4 illustrates isolation and/or purification and SDS-PAGE analysis of B3 protein. Lane 1-4: BSA control, 10 μg, 5 μg, 3 μg, 1 μg/lane respectively. Lane 5: Protein marker, 7708S NEB. Lane 6-7: B3 protein expressed for COST.

[0019] FIG. 5 depicts B3 amino acid sequence (SEQ ID NO:2).

[0020] FIG. 6 depicts DE3-1 cDNA sequence (SEQ ID NO:5).

[0021] FIG. 7 illustrates DE3-1 plasmid construction.

[0022] FIG. 8 illustrates restriction enzyme digestion of DE3-1 plasmid. Lane 1: DE3-1 in pGEX4T-1, cut with BamHI/XhoI. Lane 2: DE3-1 in pET32a, cut with BamHI/XhoI. Lane 3: 1 Kd ladder (NEB).

[0023] FIG. 9 illustrates SDS-PAGE analysis of DE3-1 expression. Lane 1: before induction. Lane 2: after induction. Lane 3: inclusion body. Lane 4: supernatant after sonication.

[0024] FIG. 10 illustrates isolation and/or purification and SDS-PAGE analysis of DE3-1 protein. Lane 1: Flow through. Lane 2-8: Eluates from NTA His tag affinity column.

[0025] FIG. 11 depicts DE3-1 amino acid sequence (SEQ ID NO:3).

[0026] FIG. 12 illustrates the effect of various vaccines on incidence of FVB/N transgenic mice.

[0027] FIG. 13 illustrates the effect of various drugs on tumor growth in mice (5 weeks).

[0028] FIG. 14 illustrates the tumor-inhibitory effect of various drugs against tumor growth (5 weeks).

[0029] FIG. 15 illustrates the effect of DE3-1 on the growth of breast cancer in mice (5 weeks).

[0030] FIG. 16 illustrates the tumor-inhibitory rate of DE3-1 against tumor growth (5 weeks).

[0031] FIG. 17 illustrates experiment on cross immunity between B2 and B3 antigen (B3 protein wrapped).

[0032] FIG. 18 illustrates experiment on cross immunity between B2 and B3 antigen (B2 protein wrapped).

[0033] FIG. 19 illustrates Result of PCR amplification

[0034] Lane 2,3: 192 bp ErbB3-f12 gene obtained by RT-PCR; Lane 1: DNA marker

[0035] FIG. 20 illustrates Screening for expression engineering strain.

[0036] FIG. 21 illustrates Experimental results of anti-tumor effect of rhErbB3-f12.

[0037] FIG. 22 illustrates Experimental results of anti-tumor effect of rhErbB3-f78.

[0038] FIG. 23 depicts ErbB3-f12 amino acid sequence (SEQ ID NO:14) and cDNA sequence (SEQ ID NO:17).

[0039] FIG. 24 depicts ErbB3-f78 cDNA sequence (SEQ ID NO:15).

[0040] FIG. 25 depicts ErbB3-f78 amino acid sequence (SEQ ID NO:16).

MODES OF CARRYING OUT THE INVENTION

[0041] For clarity of disclosure, and not by way of limitation, the detailed description of the invention is divided into the subsections that follow.

A. Definitions

[0042] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which this invention belongs. All patents, applications, published applications and other publications referred to herein are incorporated by reference in their entirety. If a definition set forth in this section is contrary to or otherwise inconsistent with a definition set forth in the patents, applications, published applications and other publications that are herein incorporated by reference, the definition set forth in this section prevails over the definition that is incorporated herein by reference.

[0043] As used herein, "a" or "an" means "at least one" or "one or more."

[0044] As used herein, "neoplasm (neoplasia)" refers to abnormal new growth, and thus means the same as tumor, which may be benign or malignant. Unlike hyperplasia, neoplastic proliferation persists even in the absence of the original stimulus.

[0045] As used herein, "cancer" refers to a general term for diseases caused by any type of malignant tumor.

[0046] As used herein, "malignant," as applies to tumors, refers to primary tumors that have the capacity of metastasis with loss of both growth control and positional control.

[0047] As used herein, "erb" refers to two oncogenes, erb A and erb B, associated with erythroblastosis virus (an acute transforming retrovirus).

[0048] As used herein, "immune response" refers to alteration in the reactivity of an organism's immune system in response to an antigen; in vertebrates, this may involve antibody production, induction of cell-mediated immunity, complement activation or development of immunological tolerance.

[0049] As used herein, "immune response potentiator" refers to a substance that enhances an antigen's effect in eliciting an immune response.

[0050] As used herein, "vaccine" refers to any compositions intended for active immunological prophylaxis. A vaccine may be used therapeutically to treat a disease, or to prevent development of a disease or to decrease the severity of a disease either proactively or after infection. Exemplary vaccines include, but are not limited to, preparations of killed microbes of virulent strains or living microbes of attenuated (variant or mutant) strains, or microbial, fungal, plant, protozoa, or metazoa derivatives or products. "Vaccine" also encompasses protein/peptide and nucleic acid/oligonucleotides based vaccines.

[0051] As used herein, "anti-neoplasm agent (used interchangeably with anti-neoplastic agent, anti-tumor or anti-cancer agent)" refers to any agents used in the anti-neoplasm treatment. These include any agents, that when used alone or in combination with other compounds, can alleviate, reduce, ameliorate, prevent, or place or maintain in a state of remission of clinical symptoms or diagnostic markers associated with neoplasm, tumor or cancer, and can be used in methods, combinations and compositions provided herein. Anti-neoplastic agents include, but are not limited to, anti-angiogenic agents, alkylating agents, antimetabolite, certain natural products, platinum coordination complexes, anthracenediones, substituted ureas, methylhydrazine derivatives, adrenocortical suppressants, certain hormones and antagonists, anti-cancer polysaccharides and certain herb extracts such as Chinese herb extracts.

[0052] As used herein, an "anti-neoplastic treatment" refers to any treatment designed to treat the neoplasm, tumor or cancer by lessening or ameliorating its symptoms. Treatments that prevent the occurrence of neoplasm, tumor or cancer or lessen its severity are also contemplated.

[0053] As used herein, "anti-neoplasm agent (or anti-tumor or anti-cancer agent) or anti-neoplasm treatment" does not encompass an ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, or use thereof for treatment, but encompasses all agents and treatment modalities known to those of skill in the art to ameliorate the symptoms in some manner of a neoplasm, tumor or cancer.

[0054] As used herein, "an effective amount of a compound for treating a particular disease" is an amount that is sufficient to ameliorate, or in some manner reduce the symptoms associated with the disease. Such amount may be administered as a single dosage or may be administered according to a regimen, whereby it is effective. The amount may cure the disease but, typically, is administered in order to ameliorate the symptoms of the disease. Repeated administration may be required to achieve the desired amelioration of symptoms.

[0055] As used herein, "treatment" means any manner in which the symptoms of a conditions, disorder or disease are ameliorated or otherwise beneficially altered. Treatment also encompasses any pharmaceutical use of the compositions herein.

[0056] As used herein, "amelioration" of the symptoms of a particular disorder by administration of a particular pharmaceutical composition refers to any lessening, whether permanent or temporary, lasting or transient that can be attributed to or associated with administration of the composition.

[0057] As used herein, "antibody" is used in the broadest sense and specifically covers intact monoclonal antibodies, polyclonal antibodies, multi-specific antibodies, e.g., bi-specific antibodies, formed from at least two intact antibodies, and antibody fragments so long as they exhibit the desired biological activity. The antibody may be an IgM, IgG, e.g., IgG₁, IgG₂, IgG₃ or IgG₄, IgD, IgA or IgE, for example.

[0058] As used herein, "antibody fragments" comprise a portion of an intact antibody, generally the antigen binding or variable region of the intact antibody. Examples of antibody fragments include Fab, Fab', F(ab')₂, and Br fragments; diabodies; single-chain antibody molecules; and multi-specific antibodies formed from antibody fragments.

[0059] As used herein, "monoclonal antibody" refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts. Monoclonal antibodies are highly specific, being directed against a single antigenic site. Furthermore, in contrast to conventional (polyclonal) antibody preparations which typically include different antibodies directed against different determinants (epitopes), each monoclonal antibody is directed against a single determinant on the antigen. In addition to their specificity, the monoclonal antibodies are advantageous in that they are synthesized by the hybridoma culture, uncontaminated by other immunoglobulins.

[0060] As used herein, "polyclonal antibody" refers to antibodies produced by several clones of B-lymphocytes as would be the case in a whole animal. Usually refers to antibodies raised in immunized animals, whereas a monoclonal antibody is the product of a single clone of B-lymphocytes, usually maintained in vitro.

[0061] As used herein, "hybridoma" refers to a cell hybrid in which a tumour cell forms one of the original source cells. Exemplary hybridoma are hybrids between T- or B-lymphocytes and appropriate myeloma cell lines that produce a monoclonal antibody.

[0062] As used herein, "humanized antibodies" refer to antibodies that are modified to include "human" sequences of amino acids so that administration to a human will not provoke an immune response. Methods for preparation of such antibodies are known. For example, the hybridoma that expresses the monoclonal antibody is altered by recombinant DNA techniques to express an antibody in which the amino acid composition of the non-variable regions is based on human antibodies. Computer programs have been designed to identify such regions.

[0063] As used herein, "production by recombinant means" refers to production methods that use recombinant nucleic acid methods that rely on well known methods of molecular biology for expressing proteins encoded by cloned nucleic acids.

[0064] As used herein, "complementary" when referring to two nucleic acid molecules, means that the two sequences of nucleotides are capable of hybridizing, preferably with less than 25%, more preferably with less than 15%, even more preferably with less than 5%, most preferably with no mismatches between opposed nucleotides. Preferably the two molecules will hybridize under conditions of high stringency.

[0065] As used herein: "stringency of hybridization" in determining percentage mismatch is as follows:

[0066] 1) high stringency: 0.1×SSPE, 0.1% SDS, 65° C.;

[0067] 2) medium stringency: 0.2×SSPE, 0.1% SDS, 50° C. (also referred to as moderate stringency); and

[0068] 3) low stringency: 1.0×SSPE, 0.1% SDS, 50° C.

It is understood that equivalent stringencies may be achieved using alternative buffers, salts and temperatures.

[0069] As used herein, "vector (or plasmid)" refers to discrete elements that are used to introduce heterologous DNA into cells for either expression or replication thereof. Selection and use of such vehicles are well known within the skill of the artisan. An expression vector includes vectors capable of expressing DNA's that are operatively linked with regulatory sequences, such as promoter regions, that are capable of effecting expression of such DNA fragments. Thus, an expression vector refers to a recombinant DNA or RNA construct, such as a plasmid, a phage, recombinant virus or other vector that, upon introduction into an appropriate host cell, results in expression of the cloned DNA. Appropriate expression vectors are well known to those of skill in the art and include those that are replicable in eukaryotic cells and/or prokaryotic cells and those that remain episomal or those which integrate into the host cell genome.

[0070] As used herein, "a promoter region or promoter element" refers to a segment of DNA or RNA that controls transcription of the DNA or RNA to which it is operatively linked. The promoter region includes specific sequences that are sufficient for RNA polymerase recognition, binding and transcription initiation. This portion of the promoter region is referred to as the promoter. In addition, the promoter region includes sequences that modulate this recognition, binding and transcription initiation activity of RNA polymerase. These sequences may be cis acting or may be responsive to trans acting factors. Promoters, depending upon the nature of the regulation, may be constitutive or regulated. Exemplary promoters contemplated for use in prokaryotes include the bacteriophage T7 and T3 promoters, and the like.

[0071] As used herein, "operatively linked or operationally associated" refers to the functional relationship of DNA with regulatory and effector sequences of nucleotides, such as promoters, enhancers, transcriptional and translational stop sites, and other signal sequences. For example, operative linkage of DNA to a promoter refers to the physical and functional relationship between the DNA and the promoter such that the transcription of such DNA is initiated from the promoter by an RNA polymerase that specifically recognizes, binds to and transcribes the DNA. In order to optimize expression and/or in vitro transcription, it may be necessary to remove, add or alter 5' untranslated portions of the clones to eliminate extra, potential inappropriate alternative translation initiation (i.e., start) codons or other sequences that may interfere with or reduce expression, either at the level of transcription or translation. Alternatively, consensus ribosome binding sites (see, e.g., Kozak, J. Biol. Chem., 266:19867-19870 (1991)) can be inserted immediately 5' of the start codon and may enhance expression. The desirability of (or need for) such modification may be empirically determined.

[0072] As used herein, "protein binding sequence" refers to a protein or peptide sequence that is capable of specific binding to other protein or peptide sequences generally, to a set of protein or peptide sequences or to a particular protein or peptide sequence.

[0073] As used herein, "epitope tag" refers to a short stretch of amino acid residues corresponding to an epitope to facilitate subsequent biochemical and immunological analysis of the "epitope tagged" protein or peptide. "Epitope tagging" is achieved by appending the sequence of the "epitope tag" to the protein-encoding sequence in an appropriate expression vector. "Epitope tagged" proteins can be affinity purified using highly specific antibodies raised against the tags.

[0074] As used herein, "Protein A or Protein G" refers to proteins that can bind to Fc region of most IgG isotypes. Protein A or Protein G are typically found in the cell wall of some strains of staphylococci. It is intended to encompass Protein A or Protein G with conservative amino acid substitutions that do not substantially alter its activity.

[0075] As used herein, "nucleotide binding sequence" refers to a protein or peptide sequence that is capable of specific binding to nucleotide sequences generally, to a set of nucleotide sequences or to a particular nucleotide sequence.

[0076] As used herein, "lipid binding sequence" refers to a protein or peptide sequence that is capable of specific binding to lipids generally, to a set of lipids or to a particular lipid.

[0077] As used herein, "polysaccharide binding sequence" refers to a protein or peptide sequence that is capable of specific binding to polysaccharides generally, to a set of polysaccharides or to a particular polysaccharide.

[0078] As used herein, "metal binding sequence" refers to a protein or peptide sequence that is capable of specific binding to metal ions generally, to a set of metal ions or to a particular metal ion.

B. Methods for Preventing, Treating or Delaying Neoplasm Using ErbB-3

[0079] In one aspect, the present invention is directed to a method for preventing, treating or delaying neoplasm in a mammal, which method comprises administering to a mammal, to which such prevention, treatment or delay is needed or desirable, an effective amount of an ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, whereby an immune response is generated against said neoplasm and said neoplasm is prevented, treated or delayed.

[0080] The present method can be used for preventing, treating or delaying neoplasm in any mammals, such as mice, rats, rabbits, cats, dogs, pigs, cows, ox, sheep, goats, horses, monkeys and other non-human primates. Preferably, the present method can be used for preventing, treating or delaying neoplasm in humans.

[0081] Any suitable ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, that can elicit an immune response to the neoplasm to be treated, prevented or delayed, can be used in the present method. The ErbB-3 elicited immune response can be cellular, humoral or both. For example, ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, disclosed in U.S. Pat. No. 5,820,859 can be used in the present method. In other examples, ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, derived from rat ErbB-3 (GenBank Accession No. U29339; and Hellyer et al., Gene, 165(2):279-284 (1995)), Fugu rubripes ErbB-3 (GenBank Accession No. AF056116; and Gellner and Brenner, Genome Res., 9(3):251-258 (1999)) and human ErbB-3 (GenBank Accession No. M29366; and Kraus et al., Proc. Natl. Acad. Sci. U.S.A., 86:9193-9197 (1989)) can be used in the present method. Preferably, ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, derived from human ErbB-3 is used in the present method. Any ErbB-3 protein, or a functional fragment thereof, with conservative amino acid substitutions that do not substantially alter its activity can be used in the present method.

[0082] In a preferred embodiment, an effective amount of an extracellular domain of an ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an extracellular domain of an ErbB-3 protein, or a functional fragment thereof, is administered. In another preferred embodiment, an effective amount of the ErbB-3 protein comprising an amino acid sequence set forth in SEQ ID NO:1 or at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14, or at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16 is administered. In still another preferred embodiment, an effective amount of the extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3, is administered.

[0083] The present method can further comprise administering an immune response potentiator to the mammal. The immune response potentiator can be administered prior to, concurrently with, or subsequent to the administration of the ErbB-3 protein, or a functional fragment thereof, or a nucleic acid an ErbB-3 protein, or a functional fragment thereof. Exemplary immune response potentiators include Bacille Calmette-Guerin (BCG) (Ratliff, Eur. Urol., 2:17-21 (1992)), Corynebacterium Parvum (Lillehoj et al., Avian Dis., 37(3):731-40 (1993)), Brucella abortus extract, glucan, levamisole, tilorone, an enzyme, a non-virulent virus, polysaccharides, and herb extracts such as Chinese herb extracts.

[0084] The formulation, dosage and route of administration of ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, preferably in the form of pharmaceutical compositions, can be determined according to the methods known in the art (see e.g., Remington: The Science and Practice of Pharmacy, Alfonso R. Gennaro (Editor) Mack Publishing Company, April 1997; Therapeutic Peptides and Proteins: Formulation, Processing, and Delivery Systems, Banga, 1999; and. Pharmaceutical Formulation Development of Peptides and Proteins, Hovgaard and Frkjr (Ed.), Taylor & Francis, Inc., 2000; Medical Applications of Liposomes, Lasic and Papahadjopoulos (Ed.), Elsevier Science, 1998; Textbook of Gene Therapy, Jain, Hogrefe & Huber Publishers, 1998; Adenoviruses: Basic Biology to Gene Therapy, Vol. 15, Seth, Landes Bioscience, 1999; Biopharmaceutical Drug Design and Development, Wu-Pong and Rojanasakul (Ed.), Humana Press, 1999; Therapeutic Angiogenesis: From Basic Science to the Clinic, Vol. 28, Dole et al. (Ed.), Springer-Verlag New York, 1999). The ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, can be formulated for oral, rectal, topical, inhalational, buccal (e.g., sublingual), parenteral (e.g., subcutaneous, intramuscular, intradermal, or intravenous), transdermal administration or any other suitable route of administration. The most suitable route in any given case will depend on the nature and severity of the condition being treated and on the nature of the particular ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, which is being used.

[0085] The ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, can be administered to any suitable place in the mammal. Preferably, the ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, is administered to the neoplasm in situ, i.e., administered to the place where the neoplasm is located or the vicinity thereof. Also, preferably, the present method can further comprise administering an immune response potentiator to the neoplasm in situ.

[0086] The ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, can be administered alone. Alternatively and preferably, the ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, is co-administered with a pharmaceutically acceptable carrier or excipient. Any suitable pharmaceutically acceptable carrier or excipient can be used in the present method (See e.g., Remington: The Science and Practice of Pharmacy, Alfonso R. Gennaro (Editor) Mack Publishing Company, April 1997).

[0087] The present method can be used alone. Alternatively, the present method can be used in combination with other anti-neoplasm treatment, e.g., radiationtherapy, chemotherapy or surgery. The present method can also be used in combination with other anti-neoplasm agent. Such other anti-neoplasm treatment or agent can be used before, with or after the administration of ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof. For example, the ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, can be co-administered with an anti-neoplasm agent.

[0088] Any suitable anti-neoplasm agent can be used in the present method. Exemplary anti-neoplasm agents include an anti-angiogenic agent (See e.g., Auerbach and Auerbach, Pharmacol. Ther., 63(3):265-311 (1994)), an alkylating agent, an antimetabolite, a natural product, a platinum coordination complex, an anthracenedione, a substituted urea, a methylhydrazine derivative, an adrenocortical suppressant, a hormone, an antagonist, an oncogene inhibitor, a tumor suppressor gene or protein, an anti-oncogene antibody and an anti-oncogene antisense oligonucleotide.

[0089] The nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, or any tumor suppressor gene can be used in the form of naked DNA, complexed DNA, cDNA, plasmid DNA, RNA or other mixtures thereof as components of the gene delivery system. In another embodiment, the nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, or the tumor suppressor gene is included in a viral vector. Any viral vectors that are suitable for gene therapy can used in the combination. For example, an adenovirus vector (U.S. Pat. No. 5,869,305), a simian virus vector (U.S. Pat. No. 5,962,274), a conditionally replicating human immunodeficiency viral vector (U.S. Pat. No. 5,888,767), retrovirus, SV40, Herpes simplex viral amplicon vectors and Vaccinia virus vectors can be used. In addition, the genes can be delivered in a non-viral vector system such as a liposome wherein the lipid protects the DNA or other biomaterials from oxidation during the coagulation.

[0090] The present method can be used to treat, prevent or delay any suitable neoplasms or cancers. Preferably, the present method is used to treat, prevent or delay any suitable neoplasms or cancers wherein the interaction between ErbB-2 and ErbB-3 is critical for causing or sustaining the neoplasms or cancers. For example, the present method can be used to treat, prevent or delay adrenal gland, anus, auditory nerve, bile ducts, bladder, bone, brain, breast, bruccal, central nervous system, cervix, colon, ear, endometrium, esophagus, eye, eyelids, fallopian tube, gastrointestinal tract, head and neck, heart, kidney, larynx, liver, lung, mandible, mandibular condyle, maxilla, mouth, nasopharynx, nose, oral cavity, ovary, pancreas, parotid gland, penis, pinna, pituitary, prostate gland, rectum, retina, salivary glands, skin, small intestine, spinal cord, stomach, testes, thyroid, tonsil, urethra, uterus, vagina, vestibulocochlear nerve and vulva neoplasm. Preferably, the present method is used to treat, prevent or delay breast, ovary, stomach, prostate, colon and lung cancer. More preferably, the present method is used to treat, prevent or delay breast cancer.

[0091] According to the present invention, the ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, alone or in combination with other agents, carriers or excipients, may be formulated for any suitable administration route, such as intracavernous injection, subcutaneous injection, intravenous injection, intramuscular injection, intradermal injection, oral or topical administration. The method may employ formulations for injectable administration in unit dosage form, in ampoules or in multidose containers, with an added preservative. The formulations may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, sterile pyrogen-free water or other solvents, before use. Topical administration in the present invention may employ the use of a foam, gel, cream, ointment, transdermal patch, or paste.

[0092] Pharmaceutically acceptable compositions and methods for their administration that may be employed for use in this invention include, but are not limited to those described in U.S. Pat. Nos. 5,736,154; 6,197,801 B1; 5,741,511; 5,886,039; 5,941,868; 6,258,374 B1; and 5,686,102.

[0093] The magnitude of a therapeutic dose in the treatment or prevention will vary with the severity of the condition to be treated and the route of administration. The dose, and perhaps dose frequency, will also vary according to age, body weight, condition and response of the individual patient.

[0094] It should be noted that the attending physician would know how to and when to terminate, interrupt or adjust therapy to lower dosage due to toxicity, or adverse effects. Conversely, the physician would also know how to and when to adjust treatment to higher levels if the clinical response is not adequate (precluding toxic side effects).

[0095] Any suitable route of administration may be used. Dosage forms include tablets, troches, cachet, dispersions, suspensions, solutions, capsules, patches, and the like. See, Remington's Pharmaceutical Sciences.

[0096] In practical use, the ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, alone or in combination with other agents, may be combined as the active in intimate admixture with a pharmaceutical carrier or excipient, such as beta-cyclodextrin and 2-hydroxy-propyl-beta-cyclodextrin, according to conventional pharmaceutical compounding techniques. The carrier may take a wide form of preparation desired for administration, topical or parenteral. In preparing compositions for parenteral dosage form, such as intravenous injection or infusion, similar pharmaceutical media may be employed, water, glycols, oils, buffers, sugar, preservatives, liposomes, and the like known to those of skill in the art. Examples of such parenteral compositions include, but are not limited to dextrose 5% w/v, normal saline or other solutions. The total dose of the ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, alone or in combination with other agents to be administered may be administered in a vial of intravenous fluid, ranging from about 1 ml to 2000 ml. The volume of dilution fluid will vary according to the total dose administered.

[0097] The invention also provides for kits for carrying out the therapeutic regimens of the invention. Such kits comprise in one or more containers therapeutically effective amounts of the ErbB-3 protein, or a functional fragment thereof, or a nucleic acid encoding an ErbB-3 protein, or a functional fragment thereof, alone or in combination with other agents, in pharmaceutically acceptable form. Preferred pharmaceutical forms would be in combination with sterile saline, dextrose solution, or buffered solution, or other pharmaceutically acceptable sterile fluid. Alternatively, the composition may be lyophilized or dessicated; in this instance, the kit optionally further comprises in a container a pharmaceutically acceptable solution, preferably sterile, to reconstitute the complex to form a solution for injection purposes. Exemplary pharmaceutically acceptable solutions are saline and dextrose solution.

[0098] In another embodiment, a kit of the invention further comprises a needle or syringe, preferably packaged in sterile form, for injecting the composition, and/or a packaged alcohol pad. Instructions are optionally included for administration of composition by a physician or by the patient.

C. Extracellular Domains of the ErbB-3 Protein and Nucleic Acids Encoding the Extracellular Domains of the ErbB-3 Protein and Uses thereof

[0099] In another aspect, the present invention is directed to an isolated nucleic acid fragment, which isolated nucleic acid fragment hybridizes, under low, middle or high stringency, with a sequence of nucleotides, or a complementary strand thereof, encoding an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14; or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16.

[0100] In a preferred embodiment, the isolated nucleic acid fragment hybridizes, under high stringency, with a sequence of nucleotides, or a complementary strand thereof, encoding an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14; or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16. In another preferred embodiment, the isolated nucleic acid fragment comprises a sequence of nucleotides, or a complementary strand thereof, encoding an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14; or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16. In still another preferred embodiment, the isolated nucleic acid fragment comprises a sequence of nucleotides, or a complementary strand thereof, set forth in SEQ ID NO:4 (FIG. 1) or SEQ ID NO:5 (FIG. 6) or an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14; or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16.

[0101] The isolated nucleic acid fragments can be in any suitable form. For example, the isolated nucleic acid fragment can comprise DNA, RNA, PNA or a derivative thereof. Alternatively, the isolated nucleic acid fragment can comprise both DNA and RNA or derivatives thereof. The isolated nucleic acid fragment can be single-stranded and be ready to be used in a hybridization analysis. Alternatively, the isolated nucleic acid fragment can be double-stranded and be denatured into single-stranded prior to the hybridization analysis.

[0102] The isolated nucleic acid fragment can comprise any kind of oligonucleotide or nucleic acid strand(s) containing genetically-coded and/or naturally occurring structures. The isolated nucleic acid fragments can comprise non-natural elements such as non-natural bases, e.g., inosine and xanthine, non-natural sugars, e.g., 2'-methoxy ribose, or non-natural phosphodiester linkages, e.g., methylphosphonates, phosphorothioates and peptides.

[0103] The isolated nucleic acid fragments can be produced by any suitable methods. For example, the isolated nucleic acid fragments can be chemically synthesized (See generally, Ausubel (Ed.) Current Protocols in Molecular Biology, 2.11. Synthesis and purification of oligonucleotides, John Wiley & Sons, Inc. (2000)), isolated from a natural source, produced by recombinant methods or a combination thereof. Preferably, the isolated nucleic acid fragments are produced by recombinant methods.

[0104] The isolated nucleic acid fragment can be labeled for various purposes, e.g., facilitating detection, purification and/or attachment to a surface. The label can be a chemical, an enzymatic, an immunogenic, a radioactive, a fluorescent, a luminescent or a FRET label.

[0105] A plasmid, which plasmid comprises the above nucleic acid fragment is also provided. A cell, which cell comprises the above plasmid is further provided. Any suitable cells can be used, e.g., bacterial cells, yeast cells, fungal cells, plant cells, insect cells, animal cells and human cells.

[0106] In still another aspect, the present invention is directed to a method for producing an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, which method comprises growing the above cells under conditions whereby the extracellular domain of the ErbB-3 protein, or a functional fragment thereof, is expressed by the cells, and recovering the expressed extracellular domain of the ErbB-3 protein, or a functional fragment thereof.

[0107] In yet another aspect, the present invention is directed to a substantially purified protein or peptide, which comprises an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14; or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16. The extracellular domain of the ErbB-3 protein, or a functional fragment thereof, can be produced by any suitable methods. For example, the extracellular domain of the ErbB-3 protein, or a functional fragment thereof can be chemically synthesized, isolated from a natural source, produced by recombinant methods or a combination thereof. Preferably, the extracellular domains of the ErbB-3 protein, or functional fragments thereof, are produced by recombinant methods.

[0108] In yet another aspect, the present invention is directed to a conjugate, which conjugate comprises: a) a protein or peptide comprising an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3; an amino acid sequence comprising at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14; or an amino acid sequence comprising at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16; and b) a facilitating agent linked to the extracellular domain of the ErbB-3 protein, or a functional fragment thereof, directly or via a linker, wherein the agent facilitates: i) affinity isolation or purification of a conjugate; ii) attachment of a conjugate to a surface; or iii) detection of a conjugate. The conjugate can be a fusion protein. Alternatively, the ErbB-3 protein, or a functional fragment thereof, and the facilitating agent can be linked by other means. When the conjugate is a fusion protein, a nucleic acid encoding the conjugate is also provided.

[0109] The conjugates can be produced by chemical conjugation, such as via thiol linkages, but are preferably produced by recombinant means as fusion proteins. In the fusion protein, the peptide or fragment thereof is linked to either the N-terminus or C-terminus of the ErbB-3 protein, or a functional fragment thereof. In chemical conjugates the peptide or fragment thereof may be linked anywhere that conjugation can be effected, and there may be a plurality of such peptides or fragments linked to a single the ErbB-3 protein, or a functional fragment thereof, or to a plurality thereof.

[0110] Conjugation can be effected by any method known to those of skill in the art. As described below, conjugation can be effected by chemical means, through covalent, ionic or any other suitable linkage. For example, the reagents and methods for conjugation as disclosed in WO 01/02600 can be used.

[0111] In some embodiments, the conjugate is a fusion protein, which can be isolated or purified through affinity binding between the protein or peptide fragment of the fusion protein and an affinity binding moiety. Any kind of affinity interaction can be used for isolating or purifying the fusion protein. The affinity interactions, such as those described herein, but not limited to, are protein/protein, protein/nucleotide, protein/lipid, protein/polysaccharide, or protein/metal interactions.

[0112] In other embodiments, the conjugate can be attached to a surface. More preferably, the conjugate can be attached to the surface through affinity binding between the facilitating agent of conjugate and an affinity binding moiety on the surface. Any kind of affinity interaction can be used for attaching the conjugate, including the protein/protein, protein/nucleotide, protein/lipid, protein/polysaccharide, or protein/metal interactions.

[0113] In yet another aspect, the present invention is directed to a pharmaceutical composition, which pharmaceutical composition comprises an isolated nucleic acid fragment which isolated nucleic acid fragment hybridizes, under low, middle or high stringency, with a sequence of nucleotides, or a complementary strand thereof, encoding an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 or at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14 or at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16 and a pharmaceutically acceptable carrier or excipient. Preferably, the isolated nucleic acid comprises a sequence of nucleotides, or a complementary strand thereof, encoding an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 or at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14 or at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16. The pharmaceutical composition can further comprise an immune response potentiator and/or an anti-neoplasm agent. Vaccines, comprising the above isolated nucleic acid fragments, alone or in combination with an immune response potentiator, are also provided.

[0114] In yet another aspect, the present invention is directed to a pharmaceutical composition, which pharmaceutical composition comprises a substantially purified protein or peptide, which comprises an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 or at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14 or at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16 and a pharmaceutically acceptable carrier or excipient. The pharmaceutical composition can further comprise an immune response potentiator and/or an anti-neoplasm agent. Vaccines, comprising the above substantially purified proteins or peptides, alone or in combination with an immune response potentiator, are also provided.

[0115] In yet another aspect, the present invention is directed to an antibody, which antibody binds to an epitope in an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 or at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14 or at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16. Preferably, the antibody binds specifically to an epitope in an extracellular domain of the ErbB-3 protein, or a functional fragment thereof, comprising an amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:3 or at least amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO:14 or at least amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16.

[0116] The antibody can be in any suitable form. For example, the antibody can a polyclonal, monoclonal, chimeric, single chain, human or humanized antibody (See e.g., U.S. Pat. No. 5,968,511). The antibody, in various forms, can be made according to any methods known in the art (See, e.g., Coligan et al. (Ed.), Current Protocols in Immunology, John Wiley & Sons, Inc. (2000)). Pharmaceutical compositions, comprising the above antibodies, alone or in combination with anti-neoplasm agent, and a pharmaceutically acceptable carrier or excipient are also provided.

D. Examples

[0117] The following are exemplary embodiments provided for illustrative purposes only. The inventor discovered the effect and method of B3, DE3-1, rhErbB3412 and rhErbB3-f78 as an anti-tumor vaccine in the treatment of human cancer such as breast cancer.

[0118] The inventor discovered that B3, DE3-1, rhErbB3412 and rhErbB3-f78 as an anti-tumor vaccine can significantly lower the incidence of the development of human cancer such as breast cancer in high-risk population.

[0119] The inventor provided a method of B3, DE3-1, rhErbB3-f12 and rhErbB3-f78 as an anti-tumor vaccine significantly lowering the incidence of the development of human cancer such as breast cancer in high-risk population.

[0120] The inventor discovered that there was significant effect of B3, DE3-1, DE3-1, rhErbB3-f12 and rhErbB3-f78 as an anti-tumor vaccine on postponing the tumor development such as human breast cancer.

[0121] The inventor discovered that there was significant inhibitory effect of B3, DE3-1, rhErbB3-f12 and rhErbB3-f78 as an anti-tumor vaccine on the tumor development such as human breast cancer.

[0122] The inventor discovered a method of inhibiting cancerous growth such as breast cancer and that was achieved through inducing immune responses.

[0123] The aforementioned cells may be a tumor cells, much probably they are human breast cancer cells and other cancerous cells with Erb2/ErbB3 over-expression.

[0124] It is ErbB3 protein antigen expressed through genetic engineering that made the aforementioned method come true; De3-1, rhErbB3-f12 and rhErbB3-178 is a protein expressed by E. Coli; B3 was an antigen of protein expressed by Eucaryotic cells or ErbB3 antigen produced by other methods, ErbB3 antigen may be ErbB3 molecule or part of a segment of the molecule.

[0125] Under a typical condition of cancer treatment such as breast cancer, ErbB3 vaccine, which is produced by different methods can inhibit tumor growth under certain dosage level.

[0126] The aforementioned cancers included breast cancer, ovary carcinoma, gastric carcinoma, and prostate carcinoma and lung cancer.

[0127] The following description will make the aforementioned invention more clear.

[0128] 1. Experimental Material and Methods

[0129] Preparation of B3, De3-1, rhErbB3-f12 and rhErbB3-f78

[0130] The vaccine involved in the present experiment included protein molecule in the extra-cell membranes region of ErbB3 and part of the protein segment of the extra-cell membranes, they are named as B2 and SD32. The protein molecule in the extra-cell membranes region of ErbB3 and part of the protein of the extra-cell membranes serves as an experiment sample, they are named as B3, De3-1, rhErbB3-f12 and rhErbB3-f78 here; the aforementioned vaccines are manufactured by Zensun (Shanghai) Science and Technology Development Co Ltd. The preparation of B3 and DE3-1, rhErbB3-f12 and rhErbB3-f78 is as follow:

[0131] Preparing B3

[0132] B3 gene is the encoded cDNA sequence of protein of ErbB3 extra-cell membrane region (FIG. 1); amplified with PCR, sequence of the primer was:

TABLE-US-00001 Primer1, (SEQ ID NO: 6) 5'TCTGCGGAGTCATGAGGGC Primer2, (SEQ ID NO: 7) 3'TCACTTGTCGTCATCGTCCTTGTAGTCTTTGCCGATCAGCACCAGTGT

[0133] The italics are flag sequence.

[0134] After PCR amplification, the targeting gene was cloned into pMD-18T vector; the transformer will be cut by BamHI/Sa1I after enzyme digestion and identified of the sequence to be correct, then connected to pCDNA3BamHI/xhol.

[0135] Establishment and screening of high performance engineering bacteria: After identification through PCR and enzyme digestion, the engineering bacteria went through 15% SDS-PAGE electrophoresis, thin layer scanning analysis, affinity chromatography, Western-blotting identification and repeated screening a stable high expressive targeting protein engineering bacteria. FIG. 4 illustrated the B3 protein purification, affinity chromatography purification. FIG. 5 showed the targeting protein and amino acid sequence of B3 purified protein after amino acid sequencing.

[0136] DE3-1 Preparation

[0137] FIG. 6 showed cDNA sequence of encoded extra-cell membrane ErbB3 protein segment of PCR amplified targeting gene. Structure of the expressed plasmid: targeting gene segment was cut out with BamHI/XhoI from pGEX4T-1 vector (Phamacia company), connecting into pET32a vector (Novagen company) BabHI/XhoI, the protein was expressed by T7 promoter, N end fused with Trx Tag, His Tag and S-Tag, FIG. 7 illustrates the diagram. FIG. 8 illustrates the identification of the plasmid composition.

[0138] DE3-1 protein expression: Transferring the plasmid into BL21 strain, inoculated the strain into 5 ml of LB+AP, over night; 1:100 inoculated into pre-warmed LB+AP, 37° C. for 2.5-3 hours (OD=0.6); induction with IPTG at 37° C. for 3 hours or 30° C. for 8 hours; centrifuge at 4° C., 6K for 10 minutes, take out the supernatant, put the sediment on ice; PBS suspension was made with cold, 1/20 bacteria solution, then crashed by ultrasound; centrifuge at 4° C., 12K for 10 minutes, large amount of 34 KD targeting gene is harvested (FIG. 9). Purified the DE3-1 protein; DE3-1 emerges in the inclusion bodies, dissolved with 6M guanidine hydrochloride, dialyzed in NTA-O buffer solution (Histag purified solution), good duplicated condition, purified with Histag affinity chromatography (bought from Bo-Cai Company) FIG. 10, after amino acid sequencing, the purified DE3-1 protein was in consistent with the targeting protein sequence, FIG. 11 showed the amino acid sequence.

[0139] rhErbB3-f12 and rhErbB3-f78 (SEQ ID NO:16) Preparation

[0140] rhErbB3-f12(SEQ ID NO:14) gene is the encoded cDNA sequence of protein of ErbB3 extra-cell membrane region; amplified with PCR, sequence of the primer was:

TABLE-US-00002 P1: 5'-TGG CCA TGG ACA TCA AGC ATA ATC GGC C-3' (1645-1664) (SEQ ID NO: 12) Ncol P2: 3'-GTG CTC GAG AGG CTC CCC ATT CAG AAA G-5' (1800-1818) (SEQ ID NO: 13) Xhol

[0141] Experiment on the Anti-Tumor Effect of B3, DE3-1

[0142] The Preventive Effect of B3, DE3-1 on Tumor Development.

[0143] 8-10 weeks old FVB/N transgenic mice (bought from Jackson Lab USA) was selected as experiment animals, the mice were divided into 5 groups with 40 mice each, they were control group, B2, B3 and DE3-1 group; BSA, B2, SD32, B3, DE3-1 was mixed with Freud's adjuvant (CFA, complete Freud's adjuvant, bought from Sigma company) and injected abdominally every 20 days for 7 injections respectively to various groups. The dosage of BSA, B2, SD32, B3 and DE3-1 vaccine was 10, 5, 10, 1 and 10 μg/mouse/injection. Weekly monitor tumour development. The tumour development was verified and analysed statistically.

[0144] Therapeutic Effect of B3, DE3-1, against Tumour

[0145] Transplanted tumour model, after immunohistological screening test, about 1000 mm³ tumour mass was cut down from spontaneous tumour of neu protein over-expressed FVB/N transgenic mice. The tumour mass was abraded into single cells with nylon net, the amount injected under the breast of each FVB/N transgenic mice was 5×10⁶ cells. About 10-14 days after inoculation, tumor was palpable (>5 mm) in the control group, demonstrating that the animal model was established successfully.

[0146] Nothing was administrated in the control group; 24 hours after the inoculation, SD32 and B3 vaccine injection started in SD32 and B3 experiment groups, the aforementioned vaccines were absorbed on 0.1 mg/ml of Al(OH)₃ respectively, and injected multi-pointedly every 2 weeks for a total of three injections; the experiment was completed in 14 days after the third injection. Morbidity was monitored weekly, tumor size was measured weekly with vernier. Volume (length diameter×short diameter 2/2) of the tumor was used to represent their size, and curve of tumor growth was protracted,

[0147] Tumor weight was measured after completion of the experiment and tumor-inhibitory rate was calculated, inhibitory rate=[(tumor weight of control group-tumor weight of experiment groups)/tumor weight of control group]×100.

[0148] Experiment on the therapeutic effect of various dosage of DE3-1 rhErbB3412 and rhErbB3-f78 on immune therapy against tumor.

[0149] Preparing animal and transplanted animal tumor model: The same as (Experiment on therapeutic effect of B3 and DE3-1 rhErbB3-f12 and rhErbB3-f78 vaccine on immune therapy against tumor). No treatment was given to the control group, histag protein was injected to the negative control group, and Adriamycin (Santou MingZhi Pharmaceuticals) was administered for the positive control group, 5 μg, 20 μg and 80 μg was given to DE3-1 group respectively.

[0150] One day after the inoculation, Adriamycin 2.2 mg/kg was injected abdominally for consecutive 7 days in mice of the positive control group; histag protein+Al(OH)³ was injected abdominally for mice of the negative control group; In DE3-1 group, the vaccine was absorbed on 0.1 mg of Al(OH)³ and multi-points subcutaneous injection every 2 weeks for a total of 3 injections were carried out in mice. The experiment completed in 14 days after the third injection. Tumor development was monitored weekly, tumor size was measured with vernier and the size was expressed as (length diameter×short diameter 2/2), curve of tumor growth was protracted and analyzed statistically.

[0151] Tumor weight was measured after completion of the experiment and tumor-inhibitory rate was calculated, tumor inhibitory rate=[(tumor weight of control group-tumor weight of experiment groups)/tumor weight of control group]×100.

[0152] Experiment on Cross Immunity of B2 and B3 Antigen

[0153] FVB transgenic mice were immuned with B2 protein and B3 protein respectively, 10 days thereafter, blood was withdrawn and antibody titer was tested with ELISA. 0.3 μg/hole of B2 and B3 was wrapped, 1:1000 B2 and B3 on each plate were titrated with standard serum respectively, cultured at 37° C. for 30 minutes, sealed with 1% BSA, added double antibody, color development for 15 minutes with DAD, tested with Bio-Rad 450 nm enzyme labeled device.

[0154] 2. Experiment Results and Discussion

[0155] Table 1 and FIG. 12 illustrate the experimental results of tumor inhibitory effect of B3 and DE3-1.

TABLE-US-00003 TABLE 1 Experimental results of tumor inhibitory effect of B3 and DE3-1 vaccine Dosage Time of Incidence (μg/ tumor of tumor Case animal/ occurrence growth Grouping number Treatment dose) (weeks) (%) Negative 40 BSA + CFA 10 19 37.5 control group B2 experiment 40 B2 + CFA 5 21 12.5 group SD32 40 SD32 + CFA 10 22 10 experiment group B3 experiment 40 B3 + CFA 5 20 12.5 group DE3-1 40 DE3-1 + 10 23 35 experiment CFA group

[0156] Objective of the present experiment is to explore whether there is preventive effect of B3 or DE3-1 vaccine on tumor development. The reason to choose this type of transgenic mice as experiment animal model, is because rat wild type neu cDNA controlled by mice breast virus promotor was transferred into the body of mice and produce over-expression of neu protein and spontaneous breast cancer occurred within 5-8 months in half of the mice. Natural course of tumor in the transgenic mice and its pathologic pattern is similar to that of human breast cancer. Therefore, it may have better therapeutic effect when used clinically. The sample contains 40 animals in each group, the aim of selecting such large sample is to ensure the number of cases which have the disorders will be greater than 10 animals, thus will be of greater implication statistically. The selection of dosage is based on the results of pre-experiments.

[0157] Transgenic mice were immunized with BSA, B2, B3, SD32 and DE3-1 respectively, as we can see from the tables and figures, the tumor incidence of 37.5% began from the 19th week on in the negative control group; whereas the time of tumor development in SD32, B3 and B2 group was 21, 22, and 20 weeks with their incidence of 10%, 12.5% and 12.5% respectively, demonstrating that there were significant tumor-inhibitory effect of SD32, B3 and B2 vaccine against the development of tumor (P<0.025; ×2 testing); at the same time, they can postpone the time of tumor development. The occurrence of tumor in DE3-1 group is later than that in the control group, however the tumor incidence of 35% was not significantly different from that of the control group (P<0.05; ×2 testing).

[0158] Experimental Results of Anti-Tumor Effect of B3 and DE3-1 Vaccine

[0159] Table 2 and FIG. 13-14 show experimental results of anti-tumor effect of B3 vaccine

TABLE-US-00004 TABLE 2 Experimental results of anti-tumor effect of B3 and DE3-1 Inhib- Tumor Tumor itory size weight rate Grouping Treatment (mm3) (g) (%) Negative histag 7849.8 ± 849.8 5.76 ± 0.55 control protein + group A1(OH)3 SD32 SD32 + A1(OH)3 4246.5 ± 540.6 3.28 ± 0.36 46 experiment group B3 B3 + A1(OH)3 5271.8 ± 658.9 3.13 ± 0.33 33 experiment group

[0160] In order to identify the anti-tumor therapeutic effect of B3, the inventor carried out experiment on immune therapy with B3 in transplanted tumor model.

[0161] Table 2 and FIG. 13-14 illustrate the effect of various vaccines on tumor growth in mice, demonstrating that the tumor-inhibitory rate of SD32 and B3 were 46% and 33% respectively, and that both of them had significant tumor-inhibitory effect (P<0.01; t testing).

[0162] Experimental Results of Anti-Tumor Effect of DE3-1, rhErbB3412 and rhErbB3478

[0163] Dosage of 5 μg, 20 μg and 80 μg/animal were used to immunized mice in the experiment group, table 3 and FIG. 15-16 showed the experimental results.

TABLE-US-00005 TABLE 3 Experimental results of anti-tumor effect of DE3-1 Case Tumor size Tumor weight Inhibitory rate Grouping number Treatment (mm3) (g) (%) Control group 8 6742.9 ± 657.8 4.769 ± 0.56 Negative 8 Histag 6476.9 ± 567.9 4.461 ± 0.52 control group protein + A1(OH)3 Positive control 8 ADR 2.2 mg/kg 4603.1 ± 478.3 3.564 ± 0.42 25.3 group DE3-1 experiment 8 80 μg DE3-1 + A1(OH)3 4810.8 ± 460.5 3.658 ± 0.37 26.3 group DE3-1 experiment 8 20 μg DE3-1 + A1(OH)3 4715.0 ± 434.8 3.455 ± 0.41 28.9 group DE3-1 experiment 8 5 μg DE3-1 + A1(OH)3 5563.7 ± 600.6 3.687 ± 0.45 22.4 group

[0164] Tumor-inhibitory rate and measured tumor size was consistent among groups with various dosage of DE3-1, the best tumor-inhibitory effect was seen in 20 μg level of DE3-1, reaching about 28.9%. After completion of the experiment, the mice were killed, took out the tumor and measured their weight; there were significant difference (P<0.001, t test) between the positive control group, groups with various dosage level, negative control group and placebo control group. The tumor-inhibitory rate of 5 μg, 20 μg and 80 μg dosage level group were 26.3%, 22.4% and 28.9% respectively.

TABLE-US-00006 TABLE 4 Experimental results of anti-tumor effect of rhErbB3-f12 Inhib- Tumor itory Case Dosage weight rate Grouping number (mg/kg) Treatment (g) (%) Negative 14 sc X 3 q14d 5.55 ± 1.25 control Positive 7 10 ip X 7 qd 3.09 ± 1.08* 44.32 control (Taxol) rhErbB3-f12 7 1 sc X 3 q14d 2.40 ± 0.49* 56.76 rhErbB3-f12 7 0.5 sc X 3 q14d 2.62 ± 0.67* 52.61 rhErbB3-f12 7 0.25 sc X 3 q14d 2.31 ± 0.40* 58.39

TABLE-US-00007 TABLE 5 Experimental results of anti-tumor effect of rhErbB3-f78 Inhib- Tumor itory Case Dosage weight rate Grouping number (mg/kg) Treatment (g) (%) Negative 14 2.5 sc X 3 q14d 1.098 ± 0.17 control Positive 7 2 ip X 7 qd 0.648 ± 0.27* 40.98 control (ADM) rhErbB3-f78 7 2.5 sc X 3 q14d 0.435 ± 0.12* 60.38

[0165] Experiment on Cross Immunity between B2 and B3 Antigen

[0166] The objective of experiment on cross immunity between B2 and B3 antigen is to explore whether there exists cross immunity between B2 and B3 antigen. FIG. 17-18 showed the experimental results demonstrating that there isn't any cross immunity between B2 and B3 antigen.

[0167] 3. Summary

[0168] In this research, we discovered new promising vaccines of B3 and DE3-1, which are designed on the basis of a new anti-tumor targeting ErbB3, and have preventive effect on tumor development and immune therapeutical effect against tumor.

[0169] The over-expression of ErbB2 receptor existed in part of adenocarcinoma discovered in the previous studies was considered to be associated with cancer development after formation of homogenous dimer. Over-expression of ErbB2 was considered to be the major cause of adenocarcinoma development, it is due to: 1) the amplification of ErbB2 gene existed in tumor cells such as breast cancer and ovary carcinoma was the cause of over-expression of ErbB2; 2) Over-expression of ErbB2 leads to phosphorylation in its cellular functional area and affects the interaction between intracellular signal molecule Shc and ErbB2; 3) the transfection of wild type ErbB2 into fibroblast can lead to cell transformation; 4) the enhancement of the formation of ErbB2 variants from ErbB2 homogenous dimer can also enhance its activity of cell transformation.

[0170] Prior to the present discovery, the inventors have discovered ErbB3 as another new anti-tumor target in addition to ErbB2. The inventors clarify that over-expression of ErbB2 receptor leads to the formation of heterogenous dimer from ErbB2 and ErbB3, and that was the cause of cancer development. Discovery of this new target provides us with new concept of anti-cancer therapeutical method: use extra-cell membrane protein of ErbB3 cells for cancer prevention and treatment, to lower the incidence of breast cancer and produce effect against tumor growth.

[0171] The tremendous success of humanized monoclonal antibody-herceptin targeted on ErbB2 is based on the relativity between over-expression of ErbB2 and occurrence of various tumors. However, the co-expression of ErbB2 and ErbB4 receptors in myocardial cells leads to the formation of heterogenous dimer from ErbB2 receptor and ErbB4 receptor; the dimer was very important in the maintain of normal structure of myocardial cells, thus, anti-cancer medicine targeting on ErbB2 receptor has damages on myocardial cells and leads to heart failure; however, anti-cancer drug targeting at ErbB3 receptor doesn't have this adverse reaction. Therefore, the use of ErbB3 as a specific anti-tumor vaccine against breast cancer, ovary carcinoma, gastrocarcinoma, prostate cancer, rectal cancer and lung cancer will play a very important role in the prevention and treatment of these cancers.

[0172] The above examples are included for illustrative purposes only and are not intended to limit the scope of the invention. Many variations to those described above are possible. Since modifications and variations to the examples described above will be apparent to those of skill in this art, it is intended that this invention be limited only by the scope of the appended claims.

Sequence CWU 1

1711342PRTHomo sapiens 1Met Arg Ala Asn Asp Ala Leu Gln Val Leu Gly Leu Leu Phe Ser Leu1 5 10 15Ala Arg Gly Ser Glu Val Gly Asn Ser Gln Ala Val Cys Pro Gly Thr 20 25 30Leu Asn Gly Leu Ser Val Thr Gly Asp Ala Glu Asn Gln Tyr Gln Thr 35 40 45Leu Tyr Lys Leu Tyr Glu Arg Cys Glu Val Val Met Gly Asn Leu Glu 50 55 60Ile Val Leu Thr Gly His Asn Ala Asp Leu Ser Phe Leu Gln Trp Ile65 70 75 80Arg Glu Val Thr Gly Tyr Val Leu Val Ala Met Asn Glu Phe Ser Thr 85 90 95Leu Pro Leu Pro Asn Leu Arg Val Val Arg Gly Thr Gln Val Tyr Asp 100 105 110Gly Lys Phe Ala Ile Phe Val Met Leu Asn Tyr Asn Thr Asn Ser Ser 115 120 125His Ala Leu Arg Gln Leu Arg Leu Thr Gln Leu Thr Glu Ile Leu Ser 130 135 140Gly Gly Val Tyr Ile Glu Lys Asn Asp Lys Leu Cys His Met Asp Thr145 150 155 160Ile Asp Trp Arg Asp Ile Val Arg Asp Arg Asp Ala Glu Ile Val Val 165 170 175Lys Asp Asn Gly Arg Ser Cys Pro Pro Cys His Glu Val Cys Lys Gly 180 185 190Arg Cys Trp Gly Pro Gly Ser Glu Asp Cys Gln Thr Leu Thr Lys Thr 195 200 205Ile Cys Ala Pro Gln Cys Asn Gly His Cys Phe Gly Pro Asn Pro Asn 210 215 220Gln Cys Cys His Asp Glu Cys Ala Gly Gly Cys Ser Gly Pro Gln Asp225 230 235 240Thr Asp Cys Phe Ala Cys Arg His Phe Asn Asp Ser Gly Ala Cys Val 245 250 255Pro Arg Cys Pro Gln Pro Leu Val Tyr Asn Lys Leu Thr Phe Gln Leu 260 265 270Glu Pro Asn Pro His Thr Lys Tyr Gln Tyr Gly Gly Val Cys Val Ala 275 280 285Ser Cys Pro His Asn Phe Val Val Asp Gln Thr Ser Cys Val Arg Ala 290 295 300Cys Pro Pro Asp Lys Met Glu Val Asp Lys Asn Gly Leu Lys Met Cys305 310 315 320Glu Pro Cys Gly Gly Leu Cys Pro Lys Ala Cys Glu Gly Thr Gly Ser 325 330 335Gly Ser Arg Phe Gln Thr Val Asp Ser Ser Asn Ile Asp Gly Phe Val 340 345 350Asn Cys Thr Lys Ile Leu Gly Asn Leu Asp Phe Leu Ile Thr Gly Leu 355 360 365Asn Gly Asp Pro Trp His Lys Ile Pro Ala Leu Asp Pro Glu Lys Leu 370 375 380Asn Val Phe Arg Thr Val Arg Glu Ile Thr Gly Tyr Leu Asn Ile Gln385 390 395 400Ser Trp Pro Pro His Met His Asn Phe Ser Val Phe Ser Asn Leu Thr 405 410 415Thr Ile Gly Gly Arg Ser Leu Tyr Asn Arg Gly Phe Ser Leu Leu Ile 420 425 430Met Lys Asn Leu Asn Val Thr Ser Leu Gly Phe Arg Ser Leu Lys Glu 435 440 445Ile Ser Ala Gly Arg Ile Tyr Ile Ser Ala Asn Arg Gln Leu Cys Tyr 450 455 460His His Ser Leu Asn Trp Thr Lys Val Leu Arg Gly Pro Thr Glu Glu465 470 475 480Arg Leu Asp Ile Lys His Asn Arg Pro Arg Arg Asp Cys Val Ala Glu 485 490 495Gly Lys Val Cys Asp Pro Leu Cys Ser Ser Gly Gly Cys Trp Gly Pro 500 505 510Gly Pro Gly Gln Cys Leu Ser Cys Arg Asn Tyr Ser Arg Gly Gly Val 515 520 525Cys Val Thr His Cys Asn Phe Leu Asn Gly Glu Pro Arg Glu Phe Ala 530 535 540His Glu Ala Glu Cys Phe Ser Cys His Pro Glu Cys Gln Pro Met Glu545 550 555 560Gly Thr Ala Thr Cys Asn Gly Ser Gly Ser Asp Thr Cys Ala Gln Cys 565 570 575Ala His Phe Arg Asp Gly Pro His Cys Val Ser Ser Cys Pro His Gly 580 585 590Val Leu Gly Ala Lys Gly Pro Ile Tyr Lys Tyr Pro Asp Val Gln Asn 595 600 605Glu Cys Arg Pro Cys His Glu Asn Cys Thr Gln Gly Cys Lys Gly Pro 610 615 620Glu Leu Gln Asp Cys Leu Gly Gln Thr Leu Val Leu Ile Gly Lys Thr625 630 635 640His Leu Thr Met Ala Leu Thr Val Ile Ala Gly Leu Val Val Ile Phe 645 650 655Met Met Leu Gly Gly Thr Phe Leu Tyr Trp Arg Gly Arg Arg Ile Gln 660 665 670Asn Lys Arg Ala Met Arg Arg Tyr Leu Glu Arg Gly Glu Ser Ile Glu 675 680 685Pro Leu Asp Pro Ser Glu Lys Ala Asn Lys Val Leu Ala Arg Ile Phe 690 695 700Lys Glu Thr Glu Leu Arg Lys Leu Lys Val Leu Gly Ser Gly Val Phe705 710 715 720Gly Thr Val His Lys Gly Val Trp Ile Pro Glu Gly Glu Ser Ile Lys 725 730 735Ile Pro Val Cys Ile Lys Val Ile Glu Asp Lys Ser Gly Arg Gln Ser 740 745 750Phe Gln Ala Val Thr Asp His Met Leu Ala Ile Gly Ser Leu Asp His 755 760 765Ala His Ile Val Arg Leu Leu Gly Leu Cys Pro Gly Ser Ser Leu Gln 770 775 780Leu Val Thr Gln Tyr Leu Pro Leu Gly Ser Leu Leu Asp His Val Arg785 790 795 800Gln His Arg Gly Ala Leu Gly Pro Gln Leu Leu Leu Asn Trp Gly Val 805 810 815Gln Ile Ala Lys Gly Met Tyr Tyr Leu Glu Glu His Gly Met Val His 820 825 830Arg Asn Leu Ala Ala Arg Asn Val Leu Leu Lys Ser Pro Ser Gln Val 835 840 845Gln Val Ala Asp Phe Gly Val Ala Asp Leu Leu Pro Pro Asp Asp Lys 850 855 860Gln Leu Leu Tyr Ser Glu Ala Lys Thr Pro Ile Lys Trp Met Ala Leu865 870 875 880Glu Ser Ile His Phe Gly Lys Tyr Thr His Gln Ser Asp Val Trp Ser 885 890 895Tyr Gly Val Thr Val Trp Glu Leu Met Thr Phe Gly Ala Glu Pro Tyr 900 905 910Ala Gly Leu Arg Leu Ala Glu Val Pro Asp Leu Leu Glu Lys Gly Glu 915 920 925Arg Leu Ala Gln Pro Gln Ile Cys Thr Ile Asp Val Tyr Met Val Met 930 935 940Val Lys Cys Trp Met Ile Asp Glu Asn Ile Arg Pro Thr Phe Lys Glu945 950 955 960Leu Ala Asn Glu Phe Thr Arg Met Ala Arg Asp Pro Pro Arg Tyr Leu 965 970 975Val Ile Lys Arg Glu Ser Gly Pro Gly Ile Ala Pro Gly Pro Glu Pro 980 985 990His Gly Leu Thr Asn Lys Lys Leu Glu Glu Val Glu Leu Glu Pro Glu 995 1000 1005Leu Asp Leu Asp Leu Asp Leu Glu Ala Glu Glu Asp Asn Leu Ala Thr 1010 1015 1020Thr Thr Leu Gly Ser Ala Leu Ser Leu Pro Val Gly Thr Leu Asn Arg1025 1030 1035 1040Pro Arg Gly Ser Gln Ser Leu Leu Ser Pro Ser Ser Gly Tyr Met Pro 1045 1050 1055Met Asn Gln Gly Asn Leu Gly Glu Ser Cys Gln Glu Ser Ala Val Ser 1060 1065 1070Gly Ser Ser Glu Arg Cys Pro Arg Pro Val Ser Leu His Pro Met Pro 1075 1080 1085Arg Gly Cys Leu Ala Ser Glu Ser Ser Glu Gly His Val Thr Gly Ser 1090 1095 1100Glu Ala Glu Leu Gln Glu Lys Val Ser Met Cys Arg Ser Arg Ser Arg1105 1110 1115 1120Ser Arg Ser Pro Arg Pro Arg Gly Asp Ser Ala Tyr His Ser Gln Arg 1125 1130 1135His Ser Leu Leu Thr Pro Val Thr Pro Leu Ser Pro Pro Gly Leu Glu 1140 1145 1150Glu Glu Asp Val Asn Gly Tyr Val Met Pro Asp Thr His Leu Lys Gly 1155 1160 1165Thr Pro Ser Ser Arg Glu Gly Thr Leu Ser Ser Val Gly Leu Ser Ser 1170 1175 1180Val Leu Gly Thr Glu Glu Glu Asp Glu Asp Glu Glu Tyr Glu Tyr Met1185 1190 1195 1200Asn Arg Arg Arg Arg His Ser Pro Pro His Pro Pro Arg Pro Ser Ser 1205 1210 1215Leu Glu Glu Leu Gly Tyr Glu Tyr Met Asp Val Gly Ser Asp Leu Ser 1220 1225 1230Ala Ser Leu Gly Ser Thr Gln Ser Cys Pro Leu His Pro Val Pro Ile 1235 1240 1245Met Pro Thr Ala Gly Thr Thr Pro Asp Glu Asp Tyr Glu Tyr Met Asn 1250 1255 1260Arg Gln Arg Asp Gly Gly Gly Pro Gly Gly Asp Tyr Ala Ala Met Gly1265 1270 1275 1280Ala Cys Pro Ala Ser Glu Gln Gly Tyr Glu Glu Met Arg Ala Phe Gln 1285 1290 1295Gly Pro Gly His Gln Ala Pro His Val His Tyr Ala Arg Leu Lys Thr 1300 1305 1310Leu Arg Ser Leu Glu Ala Thr Asp Ser Ala Phe Asp Asn Pro Asp Tyr 1315 1320 1325Trp His Ser Arg Leu Phe Pro Lys Ala Asn Ala Gly Arg Thr 1330 1335 13402640PRTHomo sapiens 2Met Arg Ala Asn Asp Ala Leu Gln Val Leu Gly Leu Leu Phe Ser Leu1 5 10 15Ala Arg Gly Ser Glu Val Gly Asn Ser Gln Ala Val Cys Pro Gly Thr 20 25 30Leu Asn Gly Leu Ser Val Thr Gly Asp Ala Glu Asn Gln Tyr Gln Thr 35 40 45Leu Tyr Lys Leu Tyr Glu Arg Cys Glu Val Val Met Gly Asn Leu Glu 50 55 60Ile Val Leu Thr Gly His Asn Ala Asp Leu Ser Phe Leu Gln Trp Ile65 70 75 80Arg Glu Val Thr Gly Tyr Val Leu Val Ala Met Asn Glu Phe Ser Thr 85 90 95Leu Pro Leu Pro Asn Leu Arg Val Val Arg Gly Thr Gln Val Tyr Asp 100 105 110Gly Lys Phe Ala Ile Phe Val Met Leu Asn Tyr Asn Thr Asn Ser Ser 115 120 125His Ala Leu Arg Gln Leu Arg Leu Thr Gln Leu Thr Glu Ile Leu Ser 130 135 140Gly Gly Val Tyr Ile Glu Lys Asn Asp Lys Leu Cys His Met Asp Thr145 150 155 160Ile Asp Trp Arg Asp Ile Val Arg Asp Arg Asp Ala Glu Ile Val Val 165 170 175Lys Asp Asn Gly Arg Ser Cys Pro Pro Cys His Glu Val Cys Lys Gly 180 185 190Arg Cys Trp Gly Pro Gly Ser Glu Asp Cys Gln Thr Leu Thr Lys Thr 195 200 205Ile Cys Ala Pro Gln Cys Asn Gly His Cys Phe Gly Pro Asn Pro Asn 210 215 220Gln Cys Cys His Asp Glu Cys Ala Gly Gly Cys Ser Gly Pro Gln Asp225 230 235 240Thr Asp Cys Phe Ala Cys Arg His Phe Asn Asp Ser Gly Ala Cys Val 245 250 255Pro Arg Cys Pro Gln Pro Leu Val Tyr Asn Lys Leu Thr Phe Gln Leu 260 265 270Glu Pro Asn Pro His Thr Lys Tyr Gln Tyr Gly Gly Val Cys Val Ala 275 280 285Ser Cys Pro His Asn Phe Val Val Asp Gln Thr Ser Cys Val Arg Ala 290 295 300Cys Pro Pro Asp Lys Met Glu Val Asp Lys Asn Gly Leu Lys Met Cys305 310 315 320Glu Pro Cys Gly Gly Leu Cys Pro Lys Ala Cys Glu Gly Thr Gly Ser 325 330 335Gly Ser Arg Phe Gln Thr Val Asp Ser Ser Asn Ile Asp Gly Phe Val 340 345 350Asn Cys Thr Lys Ile Leu Gly Asn Leu Asp Phe Leu Ile Thr Gly Leu 355 360 365Asn Gly Asp Pro Trp His Lys Ile Pro Ala Leu Asp Pro Glu Lys Leu 370 375 380Asn Val Phe Arg Thr Val Arg Glu Ile Thr Gly Tyr Leu Asn Ile Gln385 390 395 400Ser Trp Pro Pro His Met His Asn Phe Ser Val Phe Ser Asn Leu Thr 405 410 415Thr Ile Gly Gly Arg Ser Leu Tyr Asn Arg Gly Phe Ser Leu Leu Ile 420 425 430Met Lys Asn Leu Asn Val Thr Ser Leu Gly Phe Arg Ser Leu Lys Glu 435 440 445Ile Ser Ala Gly Arg Ile Tyr Ile Ser Ala Asn Arg Gln Leu Cys Tyr 450 455 460His His Ser Leu Asn Trp Thr Lys Val Leu Arg Gly Pro Thr Glu Glu465 470 475 480Arg Leu Asp Ile Lys His Asn Arg Pro Arg Arg Asp Cys Val Ala Glu 485 490 495Gly Lys Val Cys Asp Pro Leu Cys Ser Ser Gly Gly Cys Trp Gly Pro 500 505 510Gly Pro Gly Gln Cys Leu Ser Cys Arg Asn Tyr Ser Arg Gly Gly Val 515 520 525Cys Val Thr His Cys Asn Phe Leu Asn Gly Glu Pro Arg Glu Phe Ala 530 535 540His Glu Ala Glu Cys Phe Ser Cys His Pro Glu Cys Gln Pro Met Glu545 550 555 560Gly Thr Ala Thr Cys Asn Gly Ser Gly Ser Asp Thr Cys Ala Gln Cys 565 570 575Ala His Phe Arg Asp Gly Pro His Cys Val Ser Ser Cys Pro His Gly 580 585 590Val Leu Gly Ala Lys Gly Pro Ile Tyr Lys Tyr Pro Asp Val Gln Asn 595 600 605Glu Cys Arg Pro Cys His Glu Asn Cys Thr Gln Gly Cys Lys Gly Pro 610 615 620Glu Leu Gln Asp Cys Leu Gly Gln Thr Leu Val Leu Ile Gly Lys Thr625 630 635 6403190PRTHomo sapiens 3Met Arg Ala Asn Asp Ala Leu Gln Val Leu Gly Leu Leu Phe Ser Leu1 5 10 15Ala Arg Gly Ser Glu Val Gly Asn Ser Gln Ala Val Cys Pro Gly Thr 20 25 30Leu Asn Gly Leu Ser Val Thr Gly Asp Ala Glu Asn Gln Tyr Gln Thr 35 40 45Leu Tyr Lys Leu Tyr Glu Arg Cys Glu Val Val Met Gly Asn Leu Glu 50 55 60Ile Val Leu Thr Gly His Asn Ala Asp Leu Ser Phe Leu Gln Trp Ile65 70 75 80Arg Glu Val Thr Gly Tyr Val Leu Val Ala Met Asn Glu Phe Ser Thr 85 90 95Leu Pro Leu Pro Asn Leu Arg Val Val Arg Gly Thr Gln Val Tyr Asp 100 105 110Gly Lys Phe Ala Ile Phe Val Met Leu Asn Tyr Asn Thr Asn Ser Ser 115 120 125His Ala Leu Arg Gln Leu Arg Leu Thr Gln Leu Thr Glu Ile Leu Ser 130 135 140Gly Gly Val Tyr Ile Glu Lys Asn Asp Lys Leu Cys His Met Asp Thr145 150 155 160Ile Asp Trp Arg Asp Ile Val Arg Asp Arg Asp Ala Glu Ile Val Val 165 170 175Lys Asp Asn Gly Arg Ser Cys Pro Pro Cys His Glu Val Cys 180 185 19041914DNAHomo sapiens 4agggcgaacg acgctctgca ggtgctgggc ttgcttttca gcctggcccg gggctccgag 60gtgggcaact ctcaggcagt gtgtcctggg actctgaatg gcctgagtgt gaccggcgat 120gctgagaacc aataccagac actgtacaag ctctacgaga ggtgtgaggt ggtgatgggg 180aaccttgaga ttgtgctcac gggacacaat gccgacctct ccttcctgca gtggattcga 240gaagtgacag gctatgtcct cgtggccatg aatgaattct ctactctacc attgcccaac 300ctccgcgtgg tgcgagggac ccaggtctac gatgggaagt ttgccatctt cgtcatgttg 360aactataaca ccaactccag ccacgctctg cgccagctcc gcttgactca gctcaccgag 420attctgtcag ggggtgttta tattgagaag aacgataagc tttgtcacat ggacacaatt 480gactggaggg acatcgtgag ggaccgagat gctgagatag tggtgaagga caatggcaga 540agctgtcccc cctgtcatga ggtttgcaag gggcgatgct ggggtcctgg atcagaagac 600tgccagacat tgaccaagac catctgtgct cctcagtgta atggtcactg ctttgggccc 660aaccccaacc agtgctgcca tgatgagtgt gccgggggct gctcaggccc tcaggacaca 720gactgctttg cctgccggca cttcaatgac agtggagcct gtgtacctcg ctgtccacag 780cctcttgtct acaacaagct aactttccag ctggaaccca atccccacac caagtatcag 840tatggaggag tttgtgtagc cagctgtccc cataactttg tggtggatca aacatcctgt 900gtcagggcct gtcctcctga caagatggaa gtagataaaa atgggctcaa gatgtgtgag 960ccttgtgggg gactatgtcc caaagcctgt gagggaacag gctctgggag ccgcttccag 1020actgtggact cgagcaacat tgatggattt gtgaactgca ccaagatcct gggcaacctg 1080gactttctga tcaccggcct caatggagac ccctggcaca agatccctgc cctggaccca 1140gagaagctca atgtcttccg gacagtacgg gagatcacag gttacctgaa catccagtcc 1200tggccgcccc acatgcacaa cttcagtgtt ttttccaatt tgacaaccat tggaggcaga 1260agcctctaca accggggctt ctcattgttg atcatgaaga acttgaatgt cacatctctg 1320ggcttccgat ccctgaagga aattagtgct gggcgtatct atataagtgc caataggcag 1380ctctgctacc accactcttt gaactggacc aaggtgcttc gggggcctac ggaagagcga 1440ctagacatca agcataatcg gccgcgcaga gactgcgtgg cagagggcaa agtgtgtgac 1500ccactgtgct cctctggggg atgctggggc ccaggccctg gtcagtgctt gtcctgtcga 1560aattatagcc gaggaggtgt ctgtgtgacc cactgcaact ttctgaatgg ggagcctcga 1620gaatttgccc atgaggccga atgcttctcc tgccacccgg aatgccaacc catggagggc 1680actgccacat gcaatggctc gggctctgat acttgtgctc aatgtgccca ttttcgagat

1740gggccccact gtgtgagcag ctgcccccat ggagtcctag gtgccaaggg cccaatctac 1800aagtacccag atgttcagaa tgaatgtcgg ccctgccatg agaactgcac ccaggggtgt 1860aaaggaccag agcttcaaga ctgtttagga caaacactgg tgctgatcgg caaa 19145475DNAHomo sapiens 5gatcctgtcc tgggactctg aatggcctga gtgtgaccgg cgatgctgag aaccaatacc 60agacactgta caagctctac gagaggtgtg aggtggtgat ggggaacctt gagattgtgc 120tcacgggaca caatgccgac ctctccttcc tgcagtggat tcgagaagtg acaggctatg 180tcctcgtggc catgaatgaa ttctctactc taccattgcc caacctccgc gtggtgcgag 240ggacccaggt ctacgatggg aagtttgcca tcttcgtcat gttgaactat aacaccaact 300ccagccacgc tctgcgccag ctccgcttga ctcagctcac cgagattctg tcagggggtg 360tttatattga gaagaacgat aagctttgtc acatggacac aattgactgg agggacatcg 420tgagggaccg agatgctgag atagtggtga aggacaatgg cagaagctga ctcga 475619DNAArtificial SequencePrimer 6tctgcggagt catgagggc 19748DNAArtificial SequencePrimer 7tgtgaccacg actagccgtt tctgatgttc ctgctactgc tgttcact 48816DNAArtificial SequencePrimer 8tttctgcgga gtcatg 16915DNAArtificial SequencePrimer 9gacgacgacg acaag 151016DNAArtificial SequencePrimer 10gccatggctg atatcg 161123DNAArtificial SequencePrimer 11gcaccaccac caccaccact gag 231228DNAArtificial SequencePrimer 12tggccatgga catcaagcat aatcggcc 281328DNAArtificial sequencePrimer 13gaaagactta cccctcggag agctcgtg 281489PRTHomo sapiens 14Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala1 5 10 15Ala Gln Pro Ala Met Ala Met Asp Ile Lys His Asn Arg Pro Arg Arg 20 25 30Asp Cys Val Ala Glu Gly Lys Val Cys Asp Pro Leu Cys Ser Ser Gly 35 40 45Gly Cys Trp Gly Pro Gly Pro Gly Gln Cys Leu Ser Cys Arg Asn Tyr 50 55 60Ser Arg Gly Gly Val Cys Val Thr His Cys Asn Phe Leu Asn Gly Glu65 70 75 80Pro Leu Glu His His His His His His 8515456DNAHomo sapiens 15atggtttgtg tagccagctg tccccataac tttgtggtgg atcaaacatc ctgtgtcagg 60gcctgtcctc ctgacaagat ggaagtagat aaaaatgggc tcaagatgtg tgagccttgt 120gggggactat gtcccaaagc ctgtgaggga acaggctctg ggagccgctt ccagactgtg 180gactcgagca acattgatgg atttgtgaac tgcaccaaga tcctgggcaa cctggacttt 240ctgatcaccg gcctcaatgg agacccctgg cacaagatcc ctgccctgga cccagagaag 300ctcaatgtct tccggacagt acgggagatc acaggttacc tgaacatcca gtcctggccg 360ccccacatgc acaacttcag tgttttttcc aatttgacaa ccattggagg cagaaagctt 420gcggccgcac tcgagcacca ccaccaccac cactga 45616152PRTHomo sapiens 16Met Val Cys Val Ala Ser Cys Pro His Asn Phe Val Val Asp Gln Thr1 5 10 15Ser Cys Val Arg Ala Cys Pro Pro Asp Lys Met Glu Val Asp Lys Asn 20 25 30Gly Leu Lys Met Cys Glu Pro Cys Gly Gly Leu Cys Pro Lys Ala Cys 35 40 45Glu Gly Thr Gly Ser Gly Ser Arg Phe Gln Thr Val Asp Ser Ser Asn 50 55 60Ile Asp Gly Phe Val Asn Cys Thr Lys Ile Leu Gly Asn Leu Asp Phe65 70 75 80Leu Ile Thr Gly Leu Asn Gly Asp Pro Trp His Lys Ile Pro Ala Leu 85 90 95Asp Pro Glu Lys Leu Asn Val Phe Arg Thr Val Arg Glu Ile Thr Gly 100 105 110Tyr Leu Asn Ile Gln Ser Trp Pro Pro His Met His Asn Phe Ser Val 115 120 125Phe Ser Asn Leu Thr Thr Ile Gly Gly Arg Ser Lys Leu Ala Ala Ala 130 135 140Leu Glu His His His His His His145 15017270DNAHomo sapiens 17atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg 60atggccatgg acatcaagca taatcggccg cgcagagact gcgtggcaga gggcaaagtg 120tgtgacccac tgtgctcctc tgggggatgc tggggcccag gccctggtca gtgcttgtcc 180tgtcgaaatt atagccgagg aggtgtctgt gtgacccact gcaactttct gaatggggag 240cccctcgagc accaccacca ccaccactga 270

Claims

1-28. (canceled)

29. An antibody which binds to an ErbB-3 protein, wherein the ErbB-3 protein is a fragment of the extracellular domain of ErbB-3 and comprises: a) the amino acid sequence set forth in SEQ ID NO:3; or; b) amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO: 14; or c) amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16 wherein the ErbB-3 protein is not the entire extracellular domain of ErbB-3.

30. The antibody of claim 29, which is a polyclonal or monoclonal antibody.

31. The antibody of claim 29, which is a human or humanized antibody.

32-43. (canceled)

44. The antibody of claim 29, further comprising administering an immune response potentiator to the mammal.

45. The antibody of claim 29, wherein the antibody is co-administered with a pharmaceutically acceptable carrier or excipient.

46. The antibody of claim 29, wherein the antibody is co-administered with an anti-neoplasm agent.

47. The antibody of claim 46, wherein the anti-neoplasm agent is selected from the group consisting of an anti-angiogenic agent, an alkylating agent, an antimetabolite, a natural product, a platinum coordination complex, an anthracenedione, a substituted urea, a methylhydrazine derivative, an adrenocortical suppressant, a hormone, an antagonist, an oncogene inhibitor, a tumor suppressor gene or protein, an anti-oncogene antibody and an anti-oncogene antisense oligonucleotide.

48. The antibody of claim 46, wherein the neoplasm to be treated is selected from the group consisting of adrenal gland, anus, auditory nerve, bile ducts, bladder, bone, brain, breast, bruccal, central nervous system, cervix, colon, ear, endometrium, esophagus, eye, eyelids, fallopian tube, gastrointestinal tract, head and neck, heart, kidney, larynx, liver, lung, mandible, mandibular condyle, maxilla, mouth, nasopharynx, nose, oral cavity, ovary, pancreas, parotid gland, penis, pinna, pituitary, prostate gland, rectum, retina, salivary glands, skin, small intestine, spinal cord, stomach, testes, thyroid, tonsil, urethra, uterus, vagina, vestibulocochlear nerve and vulva neoplasm.

49. The antibody of claim 46, wherein the neoplasm to be treated is selected from the group consisting of breast, ovary, stomach, prostate, colon and lung cancer.

50. The antibody of claim 46, wherein the neoplasm to be treated is breast cancer.

51. The antibody of claim 29, wherein the antibody is administered by intracavernous injection, subcutaneous injection, intravenous injection, intramuscular injection, intradermal injection, oral administration or topical administration.

52. The antibody of claim 29, wherein the antibody is administered by subcutaneous injection.

53. The antibody of claim 29, wherein the antibody is administered to the neoplasm in situ.

53. The antibody of claim 44, wherein the immune response potentiator is administered to the neoplasm in situ.

54. A method for treating neoplasm which express ErbB-3 in a mammal using the antibody of claim 29.

55. A method for preparing the antibody of claim 29, wherein the method comprises: a. providing an antigen comprising the amino acid sequence set forth in SEQ ID NO:3, amino acid residues 24-81 of the amino acid sequence set forth in SEQ ID NO: 14, or amino acid residues 2-139 of the amino acid sequence set forth in SEQ ID NO:16; b. exposing an antibody repertoire or potential antibody repertoire to one of the above amino acid sequences; and c. selecting from the repertoire an antibody which specifically binds to one of the above amino acid sequences.

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