PROMOTER, PROMOTER CONTROL ELEMENTS, AND COMBINATIONS, AND USES THEREOF

Abstract

directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

Description

[0001]This application is a Divisional of co-pending application Ser. No. 11/602,163 filed on Nov. 20, 2006, and for which priority is claimed under 35 U.S.C. §120; and this application claims priority of U.S. application Ser. No. 11/172, 703, filed on Jun. 30, 2005, under 35 U.S.C. §119; which claims priority to Provisional Application No(s). 60/583,691 and 60/583,609 both filed on Jun. 30, 2004, the entire contents of each of which are hereby incorporated by reference.

FIELD OF THE INVENTION

[0002]The present invention relates to promoters and promoter control elements that are useful for modulating transcription of a desired polynucleotide. In order to modulate in vivo and in vitro transcription of a polynucleotide such promoters and promoter control elements can be included in polynucleotide constructs, expression cassettes, vectors or inserted into the chromosome or exist in the plant cell as an exogenous element. Host cells with polynucleotides comprising the promoters and promoter control elements of the present invention which have desired traits or characteristics resulting therefrom are also a part of the invention. This includes plant cells and plants regenerated therefrom.

BACKGROUND OF THE INVENTION

[0003]This invention relates to the field of biotechnology and in particular to specific promoter sequences and promoter control element sequences which are useful for the transcription of polynucleotides in a host cell or transformed host organism.

[0004]One of the primary goals of biotechnology is to obtain organisms such as plants, mammals, yeast and prokaryotes that have particular desired characteristics or traits. Examples of these characteristics or traits abound and in plants may include, for example, virus resistance, insect resistance, herbicide resistance, enhanced stability, enhanced biomass, enhanced yield or additional nutritional value.

[0005]Recent advances in genetic engineering have enabled researchers in the field to incorporate polynucleotide sequences into host cells to obtain the desired qualities in the organism of choice. This technology permits one or more polynucleotides from a source different than the organism of choice to be transcribed by the organism of choice. If desired, the transcription and/or translation of these new polynucleotides can be modulated in the organism to exhibit a desired characteristic or trait. Alternatively, new patterns of transcription and/or translation of polynucleotides endogenous to the organism can be produced. Both approaches can be used at the same time.

SUMMARY OF THE INVENTION

[0006]The present invention is directed to isolated polynucleotide sequences that comprise promoters and promoter control elements from plants, especially Arabidopsis thaliana, Glycine max, Oryza sativa and Zea mays, and other promoters and promoter control elements that function in plants.

[0007]It is an object of the present invention to provide isolated polynucleotides that are promoter sequences. These promoter sequences comprise, for example, [0008](1) a polynucleotide having a nucleotide sequence as set forth in the Sequence Listing or a fragment thereof, [0009](2) a polynucleotide having a nucleotide sequence having at least 80% sequence identity to a sequence as set in the Sequence Listing or a fragment thereof, and [0010](3) a polynucleotide having a nucleotide sequence which hybridizes to a sequence as set forth in in the Sequence Listing under a condition establishing a Tm-20° C.

[0011]It is another object of the present invention to provide isolated polynucleotides that are promoter control element sequences. These promoter control element sequences comprise, for example, [0012](1) a polynucleotide having a nucleotide sequence as set forth in the Sequence Listing or a fragment thereof, [0013](2) a polynucleotide having a nucleotide sequence having at least 80% sequence identity to a sequence as set forth in the Sequence Listing or a fragment thereof, and [0014](3) a polynucleotide having a nucleotide sequence which hybridizes to a sequence as set forth in the Sequence Listing under a condition establishing a Tm-20° C.

[0015]Promoter or promoter control element sequences of the present invention are capable of modulating preferential transcription.

[0016]In another embodiment, the present promoter control elements are capable of serving as or fulfilling the function of, for example, a core promoter, a TATA box, a polymerase binding site, an initiator site, a transcription binding site, an enhancer, an inverted repeat, a locus control region, or a scaffold/matrix attachment region.

[0017]It is yet another object of the present invention to provide a polynucleotide that includes at least a first and a second promoter control element. The first promoter control element is a promoter control element sequence as discussed above and the second promoter control element is heterologous to the first control element. Moreover, the first and second control elements are operably linked. Such promoters may modulate transcript levels preferentially in a tissue or under particular conditions.

[0018]In another embodiment, the present isolated polynucleotide comprises a promoter or a promoter control element as described above, wherein the promoter or promoter control element is operably linked to a polynucleotide to be transcribed.

[0019]In another embodiment of the present vector, the promoter and promoter control elements of the instant invention are operably linked to a heterologous polynucleotide that is a regulatory sequence.

[0020]It is another object of the present invention to provide a host cell comprising an isolated polynucleotide or vector as described above or a fragment thereof. Host cells include, for instance, bacterial, yeast, insect cells, mammalian cells and plant cells. The host cell can comprise a promoter or promoter control element exogenous to the genome. Such a promoter can modulate transcription in cis- and in trans-orientation to the polynucletide.

[0021]In yet another embodiment, the present host cell is a plant cell capable of regenerating into a plant.

[0022]It is yet another embodiment of the present invention to provide a plant comprising an isolated polynucleotide or vector described above.

[0023]It is another object of the present invention to provide a method of modulating transcription in a sample that contains either a cell-free system of transcription or a host cell. This method comprises providing a polynucleotide or vector according to the present invention as described above and contacting the sample of the polynucleotide or vector with conditions that permit transcription.

[0024]In another embodiment of the present method, the polynucleotide or vector preferentially modulates [0025](a) constitutive transcription, [0026](b) stress induced transcription, [0027](c) light or shade induced transcription, [0028](d) dark induced transcription, [0029](e) leaf transcription, [0030](f) root transcription, [0031](g) stem or shoot transcription, [0032](h) silique or seed transcription, [0033](i) callus transcription, [0034](j) flower transcription, [0035](k) immature bud and inflorescence-specific transcription, or [0036](l) senescence induced transcription [0037](m) germination transcription.Other and further objects of the present invention will be made clear or become apparent from the following description.

BRIEF DESCRIPTION OF THE TABLES AND FIGURES

Table 1

[0038]Table 1 consists of the Expression Reports for each promoter of the invention and provides the nucleotide sequence for each promoter as well as details for GFP expression driven by each of the nucleic acid promoter sequences as observed in transgenic plants. The results are presented as summaries of the spatial expression, which provides information as to gross and/or specific expression in various plant organs and tissues. The observed expression pattern is also presented, which gives details of expression during different generations or different developmental stages within a generation. Additional information is provided regarding the associated gene, the GenBank reference, the source organism of the promoter and the vector and marker genes used for the construct. The following symbols are used consistently throughout the Table: [0039]T1: First generation transformant [0040]T2: Second generation transformant [0041]T3: Third generation transformant [0042](L): low expression level [0043](M): medium expression level [0044](H): high expression level

[0045]Each row of the table begins with heading of the data to be found in the section. The following provides a description of the data to be found in each section:

TABLE-US-00001 Heading in Table 1 Description Promoter Expression Report Identifies the particular promoter report Promoter tested in Identifies the organism used for analysis Spatial expression summary: Identifies the organs and tissues where expression was observed and estimates the strength of expression Observed expression pattern: Presents expression pattern observed for various generations of plants and developmental stages Expected expression pattern: Identifies the pattern expected from other experiments Selection Criteria: Provides details on cloning the polynucleotide Gene: Provides information concerning the gene modulated by the promoter GenBank: This field gives the Locus Number of the gene as well as the accession number. Source Promoter Organism: Identifies the organism from which the promoter was cloned. Vector: Identifies the vector into which the promoter was cloned. Marker Type: Identifies the type of marker linked to the promoter. The marker is used to determine patterns of gene expression in plant tissue. Generation screened: T1 Mature T2 Identifies the plant generation(s) used in the Seedling T2 Mature T3 Seedling screening process. T1 plants are those plants subjected to the transformation event while the T2 generation plants are from the seeds collected from the T1 plants and T3 plants are from the seeds of T2 plants. Plant Expression Identifies the generation and developmental stage of the plants analyzed Events Screened Events Expressing Provides the number of independent transformation events analyzed and the number which expressed the marker gene GFP Expression Detected This section lists the various organs analyzed and, where expression was observed, indicates the strength of the expression X in the . . . This field summarizes the expression pattern from digital images of the cells Promoter Utility: Identifies a specific function or functions that can be modulated using the promoter cDNA. Trait-Subtrait Area: Provides information as to what agronomic traits could be altered Construct: Provides the Ceres identifier number for the construct Promoter Candidate I.D.: Provides the Ceres identifier number for the promoter isolated cDNA ID: Provides the Ceres identifier number associated with the cDNA that corresponds to the endogenous cDNA sequence of the promoter. T1 lines expressing (T2 seed): Provides the identifier numbers for the events analyzed Sequence Provides the nucleotide sequence for the promoter described in the report

Table 2

[0046]Table 2 provides a partial summary of the expression for some of the constructs of the invention.

[0047]FIG. 1 is a schematic representation of the vector pNewBin4-HAP1-GFP. The definitions of the abbreviations used in the vector map are as follows: [0048]Ori--the origin of replication used by an E. coli host [0049]RB--sequence for the right border of the T-DNA from pMOG800 [0050]BstXI--restriction enzyme cleavage site used for cloning [0051]HAP1VP16--coding sequence for a fusion protein of the HAP1 and VP16 activation domains [0052]NOS--terminator region from the nopaline synthase gene [0053]HAP1UAS--the upstream activating sequence for HAP1 [0054]5ERGFP--the green fluorescent protein gene that has been optimized for localization to the endoplasmic reticulum [0055]OCS2--the terminator sequence from the octopine synthase 2 gene [0056]OCS--the terminator sequence from the octopine synthase gene [0057]p28716 (a.k.a 28716 short)--promoter used to drive expression of the PAT (BAR) gene [0058]PAT (BAR)--a marker gene conferring herbicide resistance [0059]LB--sequence for the left border of the T-DNA from pMOG800 [0060]Spec--a marker gene conferring spectinomycin resistance [0061]TrfA--transcription repression factor gene [0062]RK2-OriV--origin of replication for Agrobacterium

DETAILED DESCRIPTION OF THE INVENTION

1. Definitions

[0063]Chimeric: The term "chimeric" is used to describe polynucleotides or genes, as defined supra, or constructs wherein at least two of the elements of the polynucleotide or gene or construct are heterologous to each other, such as the promoter and the polynucleotide to be transcribed and/or other regulatory sequences and/or filler sequences and/or complements thereof.

[0064]Constitutive Promoter: Promoters referred to herein as "constitutive promoters" actively promote transcription under most, but not necessarily all, environmental conditions and is essentially all cells in the vegetative stage and/or flowers and essentially all states of cell differentiation. Examples of constitutive promoters include the cauliflower mosaic virus (CaMV) 35S transcript initiation region and the 1' or 2' promoter derived from T-DNA of Agrobacterium tumefaciens as well as other transcription initiation regions from various plant genes known to those of skill in the art, such as the maize ubiquitin-1 promoter.

[0065]Core Promoter: This is the minimal stretch of contiguous DNA sequence that is sufficient to direct accurate initiation of transcription by the RNA polymerase II machinery (for review see: Struhl, 1987, Cell 49: 295-297; Smale, 1994, In Transcription: Mechanisms and Regulation (eds R. C. Conaway and J. W. Conaway), pp 63-81/Raven Press, Ltd., New York; Smale, 1997, Biochim. Biophys. Acta 1351: 73-88; Smale et al., 1998, Cold Spring Harb. Symp. Quant. Biol. 58: 21-31; Smale, 2001, Genes & Dev. 15: 2503-2508; Weis and Reinberg, 1992, FASEB J. 6: 3300-3309; Burke et al., 1998, Cold Spring Harb. Symp. Quant. Biol 63: 75-82). There are several sequence motifs, including the TATA box, initiator (Inr), TFIIB recognition element (BRE) and downstream core promoter element (DPE), that are commonly found in core promoters. Not all of these elements, however, occur in all promoters. That is, there are no universal core promoter elements (Butler and Kadonaga, 2002, Genes & Dev. 16: 2583-2592).

[0066]Domain: Domains are fingerprints or signatures that can be used to characterize protein families and/or parts of proteins. Such fingerprints or signatures can comprise conserved (1) primary sequence, (2) secondary structure, and/or (3) three-dimensional conformation. A similar analysis can be applied to polynucleotides. Generally, each domain has been associated with either a conserved primary sequence or a sequence motif. Generally these conserved primary sequence motifs have been correlated with specific in vitro and/or in vivo activities. A domain can be any length, including the entirety of the polynucleotide to be transcribed. Examples of amino acid domains include, without limitation, AP2, helicase, homeobox, zinc finger, etc. Examples of nucleotide domains include, without limitation, TATA box, CAAT box, etc.

[0067]Endogenous: The term "endogenous" within the context of the current invention refers to any polynucleotide, polypeptide or protein sequence which is a natural part of a cell or organism regenerated from said cell. In the context of promoter, the term "endogenous coding region" or "endogenous cDNA" refers to the coding region that is naturally operably linked to the promoter.

[0068]Enhancer/Suppressor: An "enhancer" is a DNA regulatory element that can increase the steady state level of a transcript, usually by increasing the rate of transcription initiation. Enhancers usually exert their effect regardless of the distance, upstream or downstream location, or orientation of the enhancer relative to the start site of transcription. In contrast, a "suppressor" is a corresponding DNA regulatory element that decreases the steady state level of a transcript, again usually by affecting the rate of transcription initiation. The essential activity of enhancer and suppressor elements is to bind a protein factor(s). Such binding can be assayed, for example, by methods described below. The binding is typically in a manner that influences the steady state level of a transcript in a cell or in an in vitro transcription extract.

[0069]Exogenous: As referred to within, "exogenous" is any polynucleotide, polypeptide or protein sequence, whether chimeric or not, that is introduced, into the genome of a host cell or organism regenerated from said host cell by any means other than by a sexual cross. Examples of means by which this can be accomplished are described below and include Agrobacterium-mediated transformation (of dicots--e.g. Salomon et al. EMBO J. 3:141 (1984); Herrera-Estrella et al. EMBO J. 2:987 (1983); of monocots, representative papers are those by Escudero et al., Plant J. 10:355 (1996), Ishida et al., Nature Biotechnology 14:745 (1996), May et al., Bio/Technology 13:486 (1995)), biolistic methods (Armaleo et al., Current Genetics 17:97 1990)), electroporation, in planta techniques and the like. Such a plant containing the exogenous nucleic acid is referred to here as a T₀ for the primary transgenic plant and T₁ for the first generation transformant. The term "exogenous" as used herein is also intended to encompass inserting a naturally found element into a non-naturally found location.

[0070]Gene: The term "gene," as used in the context of the current invention, encompasses all regulatory and coding sequence contiguously associated with a single hereditary unit with a genetic function (see SCHEMATIC 1). Genes can include non-coding sequences that modulate the genetic function that include, but are not limited to, those that specify polyadenylation, to transcriptional regulation, DNA conformation, chromatin conformation, extent and position of base methylation and binding sites of proteins that control all of these. Genes encoding proteins are comprised of "exons" (coding sequences), which may be interrupted by "introns" (non-coding sequences). In some instances complexes of a plurality of protein or nucleic acids or other molecules, or of any two of the above, may be required for a gene's function. On the other hand a gene's genetic function may require only RNA expression or protein production, or may only require binding of proteins and/or nucleic acids without associated expression. In certain cases, genes adjacent to one another may share sequence in such a way that one gene will overlap the other. A gene can be found within the genome of an organism, in an artificial chromosome, in a plasmid, in any other sort of vector, or as a separate isolated entity.

[0071]Heterologous Sequences: "Heterologous sequences" are those that are not operatively linked or are not contiguous to each other in nature. For example, a promoter from corn is considered heterologous to an Arabidopsis coding region sequence. Also, a promoter from a gene encoding a growth factor from corn is considered heterologous to a sequence encoding the corn receptor for the growth factor. Regulatory element sequences, such as UTRs or 3' end termination sequences that do not originate in nature from the same gene as the coding sequence originates from, are considered heterologous to said coding sequence. Elements operatively linked in nature and contiguous to each other are not heterologous to each other.

[0072]Homologous: In the current invention, a "homologous" gene or polynucleotide or polypeptide refers to a gene or polynucleotide or polypeptide that shares sequence similarity with the gene or polynucleotide or polypeptide of interest. This similarity may be in only a fragment of the sequence and often represents a functional domain such as, examples including without limitation a DNA binding domain or a domain with tyrosine kinase activity. The functional activities of homologous polynucleotide are not necessarily the same.

[0073]Inducible Promoter: An "inducible promoter" in the context of the current invention refers to a promoter the activity of which is influenced by certain conditions such as light, temperature, chemical concentration, protein concentration, conditions in an organism, cell, or organelle, etc. A typical example of an inducible promoter, which can be utilized with the polynucleotides of the present invention, is PARSK1, the promoter from an Arabidopsis gene encoding a serine-threonine kinase enzyme which is induced by dehydration, abscissic acid and sodium chloride (Wang and Goodman, Plant J. 8:37 (1995)). Examples of environmental conditions that may affect transcription by inducible promoters include anaerobic conditions, elevated temperature, the presence or absence of a nutrient or other chemical compound and/or the presence of light.

[0074]Modulate Transcription Level: As used herein, the phrase "modulate transcription" describes the biological activity of a promoter sequence or promoter control element. Such modulation includes, without limitation, up- and down-regulation of initiation of transcription, rate of transcription and/or transcription levels.

[0075]Mutant: In the current invention "mutant" refers to a heritable change in a mutation sequence at a specific location. Mutant genes of the current invention may or may not have an associated identifiable phenotype.

[0076]Operable Linkage: An "operable linkage" is a linkage in which a promoter sequence or promoter control element is connected to a polynucleotide sequence(s) in such a way as to place transcription of the polynucleotide sequence under the influence or control of the promoter or promoter control element. Two DNA sequences (such as a polynucleotide to be transcribed and a promoter sequence linked to the 5' end of the polynucleotide to be transcribed) are said to be operably linked if induction of promoter function results in the transcription of mRNA encoded by the polynucleotide and if the nature of the linkage between the two DNA sequences does not (1) result in the introduction of a frame-shift mutation, (2) interfere with the ability of the promoter sequence to direct the expression of the protein, antisense RNA or ribozyme or (3) interfere with the ability of the DNA template to be transcribed. Thus, a promoter sequence would be operably linked to a polynucleotide sequence if the promoter was capable of effecting transcription of that polynucleotide sequence.

[0077]Optional Promoter Fragments: The phrase "optional promoter fragments" is used to refer to any sub-sequence of the promoter that is not required for driving transcription of an operationally linked coding region. These fragments comprise the 5' UTR and any exon(s) of the endogenous coding region. The optional promoter fragments may also comprise any exon(s) and the 3' or 5' UTR of the gene residing upstream of the promoter (that is, 5' to the promoter). Optional promoter fragments also include any intervening sequences that are introns or sequence that occurs between exons or an exon and the UTR.

[0078]Orthologous: "Orthologous" is a term used herein to describe a relationship between two or more polynucleotides or proteins. Two polynucleotides or proteins are "orthologous" to one another if they serve a similar function in different organisms. In general, orthologous polynucleotides or proteins will have similar catalytic functions (when they encode enzymes) or will serve similar structural functions (when they encode proteins or RNA that form part of the ultrastructure of a cell). Generally it is believed that orthologous structures share a common evolutionary origin.

[0079]Percentage of Sequence Identity: "Percentage of sequence identity," as used herein, is determined by comparing two optimally aligned sequences over a comparison window, where the fragment of the polynucleotide or amino acid sequence in the comparison window may comprise additions or deletions (e.g., gaps or overhangs) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison and multiplying the result by 100 to yield the percentage of sequence identity. Optimal alignment of sequences for comparison may be conducted by the local homology algorithm of Smith and Waterman Add. APL. Math. 2:482 (1981), by the homology alignment algorithm of Needleman and Wunsch J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson and Lipman Proc. Natl. Acad. Sci. (USA) 85: 2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, BLAST, PASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group (GCG), 575 Science Dr., Madison, Wis.), or by inspection. Given that two sequences have been identified for comparison, GAP and BESTFIT are preferably employed to determine their optimal alignment. Typically, the default values of 5.00 for gap weight and 0.30 for gap weight length are used. The term "substantial sequence identity" between polynucleotide or polypeptide sequences refers to polynucleotide or polypeptide comprising a sequence that has at least 80% sequence identity, preferably at least 85%, more preferably at least 90% and most preferably at least 95%, even more preferably, at least 96%, 97%, 98% or 99% sequence identity compared to a reference sequence using the programs.

[0080]Query nucleic acid sequences were searched against subject nucleic acid sequences residing in public or proprietary databases. Such searches were done using the Washington University Basic Local Alignment Search Tool Version 1.83 (WU-Blast2) program. The WU-Blast2 program is available on the internet from Washington University. A WU-Blast2 service for Arabidopsis can also be found on the internet. Typically the following parameters of WU-Blast2 were used: Filter options were set to "default," Output format was set to "gapped alignments," the Comparison Matrix was set to "BLOSUM62," Cutoff Score (S value) was set to "default," the Expect (E threshold) was set to "default," the Number of best alignments to show was set to "100," and the "Sort output" option was set to sort the output by "pvalue."

[0081]Plant Promoter: A "plant promoter" is a promoter capable of initiating transcription in plant cells and can modulate transcription of a polynucleotide. Such promoters need not be of plant origin. For example, promoters derived from plant viruses, such as the CaMV35S promoter or from Agrobacterium tumefaciens such as the T-DNA promoters, can be plant promoters. A typical example of a plant promoter of plant origin is the maize ubiquitin-1 (ubi-1) promoter known to those of skill in the art.

[0082]Plant Tissue: The term "plant tissue" includes differentiated and undifferentiated tissues or plants, including but not limited to roots, stems, shoots, cotyledons, epicotyl, hypocotyl, leaves, pollen, seeds, tumor tissue and various forms of cells in culture such as single cells, protoplast, embryos, and callus tissue. The plant tissue may be in plants or in organ, tissue or cell culture.

[0083]Preferential Transcription: "Preferential transcription" is defined as transcription that occurs in a particular pattern of cell types or developmental times or in response to specific stimuli or combination thereof. Non-limitive examples of preferential transcription include: high transcript levels of a desired sequence in root tissues; detectable transcript levels of a desired sequence in certain cell types during embryogenesis; and low transcript levels of a desired sequence under drought conditions. Such preferential transcription can be determined by measuring initiation, rate, and/or levels of transcription.

[0084]Promoter: A "promoter" is a DNA sequence that directs the transcription of a polynucleotide. Typically a promoter is located in the 5' region of a polynucleotide to be transcribed, immediately upstream to the transcriptional start site of such polynucleotide. More typically, promoters are defined as the region upstream of the first exon; more typically, as a region upstream of the first of multiple transcription start sites; more typically, as the region downstream of the preceding gene and upstream of the first of multiple transcription start sites; more typically, the region downstream of the polyA signal and upstream of the first of multiple transcription start sites; even more typically, about 3,000 nucleotides upstream of the ATG of the first exon; even more typically, 2,000 nucleotides upstream of the first of multiple transcription start sites. The promoters of the invention comprise at least a core promoter as defined above. Frequently promoters are capable of directing transcription of genes located on each of the complementary DNA strands that are 3' to the promoter. Stated differently, many promoters exhibit bidirectionality and can direct transcription of a downstream gene when present in either orientation (i.e. 5' to 3' or 3' to 5' relative to the coding region of the gene). Additionally, the promoter may also include at least one control element such as an upstream element. Such elements include UARs and optionally, other DNA sequences that affect transcription of a polynucleotide such as a synthetic upstream element.

[0085]Promoter Control Element: The term "promoter control element" as used herein describes elements that influence the activity of the promoter. Promoter control elements include transcriptional regulatory sequence determinants such as, but not limited to, enhancers, scaffold/matrix attachment regions, TATA boxes, transcription start locus control regions, UARs, URRs, other transcription factor binding sites and inverted repeats.

[0086]Public Sequence: The term "public sequence," as used in the context of the instant application, refers to any sequence that has been deposited in a publicly accessible database prior to the filing date of the present application. This term encompasses both amino acid and nucleotide sequences. Such sequences are publicly accessible, for example, on the BLAST databases on the NCBI FTP web site (accessible via the worldwide web). The database at the NCBI FTP site uses "gi" numbers assigned by NCBI as a unique identifier for each sequence in the database, thereby providing a non-redundant database for sequence from various databases, including GenBank, EMBL, DBBJ, (DNA Database of Japan) and PDB (Brookhaven Protein Data Bank).

[0087]Regulatory Sequence: The term "regulatory sequence," as used in the current invention, refers to any nucleotide sequence that influences transcription or translation initiation and rate, or stability and/or mobility of a transcript or polypeptide product. Regulatory sequences include, but are not limited to, promoters, promoter control elements, protein binding sequences, 5' and 3' UTRs, transcriptional start sites, termination sequences, polyadenylation sequences, introns, certain sequences within amino acid coding sequences such as secretory signals, protease cleavage sites, etc.

[0088]Related Sequences: "Related sequences" refer to either a polypeptide or a nucleotide sequence that exhibits some degree of sequence similarity with a reference sequence.

[0089]Specific Promoters: In the context of the current invention, "specific promoters" refers to a subset of promoters that have a high preference for modulating transcript levels in a specific tissue or organ or cell and/or at a specific time during development of an organism. By "high preference" is meant at least 3-fold, preferably 5-fold, more preferably at least 10-fold still more preferably at least 20-fold, 50-fold or 100-fold increase in transcript levels under the specific condition over the transcription under any other reference condition considered. Typical examples of temporal and/or tissue or organ specific promoters of plant origin that can be used with the polynucleotides of the present invention, are: PTA29, a promoter which is capable of driving gene transcription specifically in tapetum and only during anther development (Koltonow et al., Plant Cell 2:1201 (1990); RCc2 and RCc3, promoters that direct root-specific gene transcription in rice (Xu et al., Plant Mol. Biol. 27:237 (1995); and TobRB27, a root-specific promoter from tobacco (Yamamoto et al., Plant Cell 3:371 (1991)). Examples of tissue-specific promoters under developmental control include promoters that initiate transcription only in certain tissues or organs, such as root, ovule, fruit, seeds, or flowers. Other specific promoters include those from genes encoding seed storage proteins or the lipid body membrane protein, oleosin. A few root-specific promoters are noted above. See also "Preferential transcription".

[0090]Stringency: "Stringency" as used herein is a function of probe length, probe composition (G+C content) and salt concentration, organic solvent concentration and temperature of hybridization or wash conditions. Stringency is typically compared by the parameter T_m, which is the temperature at which 50% of the complementary molecules in the hybridization are hybridized, in terms of a temperature differential from T_m. High stringency conditions are those providing a condition of T_m-5° C. to T_m-10° C. Medium or moderate stringency conditions are those providing T_m-20° C. to T_m-29° C. Low stringency conditions are those providing a condition of T_m-40° C. to T_m-48° C. The relationship of hybridization conditions to T_m (in ° C.) is expressed in the mathematical equation

T_m=81.5-16.6(log₁₀ [Na.sup.+])+0.41(% G+C)-(600/N) (1)

where N is the length of the probe. This equation works well for probes 14 to 70 nucleotides in length that are identical to the target sequence. The equation below for T_m of DNA-DNA hybrids is useful for probes in the range of 50 to greater than 500 nucleotides, and for conditions that include an organic solvent (formamide).

T_m=81.5+16.6 log {[Na.sup.+]/(1+0.7[Na.sup.+])}+0.41 (% G+C)-500/L 0.63 (% formamide) (2)

where L is the length of the probe in the hybrid (P. Tijessen, "Hybridization with Nucleic Acid Probes" in Laboratory Techniques in Biochemistry and Molecular Biology, P.C. vand der Vliet, ed., c. 1993 by Elsevier, Amsterdam.) The T_m of equation (2) is affected by the nature of the hybrid; for DNA-RNA hybrids T_m is 10-15° C. higher than calculated, for RNA-RNA hybrids T_m is 20-25° C. higher. Because the T_m decreases about 1° C. for each 1% decrease in homology when a long probe is used (Bonner et al. (1973) J. Mol. Biol: 81:123), stringency conditions can be adjusted to favor detection of identical genes or related family members.

[0091]Equation (2) is derived assuming equilibrium and therefore, hybridizations according to the present invention are most preferably performed under conditions of probe excess and for sufficient time to achieve equilibrium. The time required to reach equilibrium can be shortened by inclusion of a hybridization accelerator such as dextran sulfate or another high volume polymer in the hybridization buffer.

[0092]Stringency can be controlled during the hybridization reaction or after hybridization has occurred by altering the salt and temperature conditions of the wash solutions used. The formulas shown above are equally valid when used to compute the stringency of a wash solution. Preferred wash solution stringencies lie within the ranges stated above; high stringency is 5-8° C. below T_m, medium or moderate stringency is 26-29° C. below T_m and low stringency is 45-48° C. below T_m.

[0093]Substantially Free of: A composition containing A is "substantially free of" B when at least 85% by weight of the total A+B in the composition is A. Preferably, A comprises at least about 90% by weight of the total of A+B in the composition, more preferably at least about 95% or even 99% by weight. For example, a plant gene can be substantially free of other plant genes. Other examples include, but are not limited to, ligands substantially free of receptors (and vice versa), a growth factor substantially free of other growth factors and a transcription binding factor substantially free of nucleic acids.

[0094]Suppressor: See "Enhancer/Suppressor"

[0095]TATA to Start: "TATA to start" shall mean the distance, in number of nucleotides, between the primary TATA motif and the start of transcription.

[0096]Transgenic Plant: A "transgenic plant" is a plant having one or more plant cells that contain at least one exogenous polynucleotide introduced by recombinant nucleic acid methods.

[0097]Translational Start Site: In the context of the present invention, a "translational start site" is usually an ATG or AUG in a transcript, often the first ATG or AUG. A single protein encoding transcript, however, may have multiple translational start sites.

[0098]Transcription Start Site: "Transcription start site" is used in the current invention to describe the point at which transcription is initiated. This point is typically located about 25 nucleotides downstream from a TFIID binding site, such as a TATA box. Transcription can initiate at one or more sites within the gene and a single polynucleotide to be transcribed may have multiple transcriptional start sites, some of which may be specific for transcription in a particular cell-type or tissue or organ. "+1" is stated relative to the transcription start site and indicates the first nucleotide in a transcript.

[0099]Upstream Activating Region (UAR): An "Upstream Activating Region" or "UAR" is a position or orientation dependent nucleic acid element that primarily directs tissue, organ, cell type, or environmental regulation of transcript level, usually by affecting the rate of transcription initiation. Corresponding DNA elements that have a transcription inhibitory effect are called herein "Upstream Repressor Regions" or "URR"s. The essential activity of these elements is to bind a protein factor. Such binding can be assayed by methods described below. The binding is typically in a manner that influences the steady state level of a transcript in a cell or in vitro transcription extract.

[0100]Untranslated Region (UTR): A "UTR" is any contiguous series of nucleotide bases that is transcribed, but is not translated. A 5' UTR lies between the start site of the transcript and the translation initiation codon and includes the +1 nucleotide. A 3' UTR lies between the translation termination codon and the end of the transcript. UTRs can have particular functions such as increasing mRNA message stability or translation attenuation. Examples of 3' UTRs include, but are not limited to polyadenylation signals and transcription termination sequences.

[0101]Variant: The term "variant" is used herein to denote a polypeptide or protein or polynucleotide molecule that differs from others of its kind in some way. For example, polypeptide and protein variants can consist of changes in amino acid sequence and/or charge and/or post-translational modifications (such as glycosylation, etc). Likewise, polynucleotide variants can consist of changes that add or delete a specific UTR or exon sequence. It will be understood that there may be sequence variations within sequence or fragments used or disclosed in this application. Preferably, variants will be such that the sequences have at least 80%, preferably at least 90%, 95, 97, 98, or 99% sequence identity. Variants preferably measure the primary biological function of the native polypeptide or protein or polynucleotide.

2. Introduction

[0102]The polynucleotides of the invention comprise promoters and promoter control elements that are capable of modulating transcription.

[0103]Such promoters and promoter control elements can be used in combination with native or heterologous promoter fragments, control elements or other regulatory sequences to modulate transcription and/or translation.

[0104]Specifically, promoters and control elements of the invention can be used to modulate transcription of a desired polynucleotide, which includes without limitation: [0105](a) antisense; [0106](b) ribozymes; [0107](c) coding sequences; or [0108](d) fragments thereof.The promoter also can modulate transcription in a host genome in cis- or in trans-.

[0109]In an organism such as a plant, the promoters and promoter control elements of the instant invention are useful to produce preferential transcription which results in a desired pattern of transcript levels in particular cells, tissues or organs or under particular conditions.

3. Table of Contents

[0110]The following description of the present invention is outlined in the following table of contents.

[0111]A. Identifying and Isolating Promoter Sequences of the Invention [0112](1) Cloning Methods [0113](2) Chemical Synthesis

[0114]B. Isolating Related Promoter Sequences [0115](1) Relatives Based on Nucleotide Sequence Identity [0116](2) Relatives Based on Coding Sequence Identity [0117](3) Relatives Based on Common Function

[0118]C. Identifying Control Elements [0119](1) Types of Transcription Control Elements [0120](2) Those Described by the Examples [0121](3) Those Identifiable by Bioinformatics [0122](4) Those Identifiable by In Vitro and In Vivo Assays [0123](5) Non-Natural Control Elements

[0124]D. Constructing Promoters and Control Elements [0125](1) Combining Promoters and Promoter Control Elements [0126](2) Number of Promoter Control Elements [0127](3) Spacing Between Control Elements

[0128]E. Vectors [0129](1) Modification of Transcription by Promoters and Promoter Control Elements [0130](2) Polynucleotide to be Transcribed [0131](3) Other Regulatory Elements [0132](4) Other Components of Vectors

[0133]F. Insertion of Polynucleotides and Vectors Into a Host Cell [0134](1) Autonomous of the Host Genome [0135](2) Integrated into the Host Genome

[0136]G. Utility

A. Identifying and Isolating Promoter Sequences of the Invention

[0137]The promoters and promoter control elements of the present invention are presented in the Sequence Listing and were identified from Arabidopsis thaliana or Oryza saliva. Additional promoter sequences encompassed by the invention can be identified as described below.

[0138](1) Cloning Methods

[0139]Isolation from genomic libraries of polynucleotides comprising the sequences of the promoters and promoter control elements of the present invention is possible using known techniques.

[0140]For example, polymerase chain reaction (PCR) can amplify the desired polynucleotides utilizing primers designed from sequences in the row titled "Sequences". Polynucleotide libraries comprising genomic sequences can be constructed according to Sambrook et al., Molecular Cloning: A Laboratory Manual, 2^nd Ed. (1989) Cold Spring Harbor Press, Cold Spring Harbor, N.Y.), for example.

[0141]Other procedures for isolating polynucleotides comprising the promoter sequences of the invention include, without limitation, tail-PCR and 5' rapid amplification of cDNA ends (RACE). For tail-PCR see, for example, Liu et al. (1995) Plant J 8 (3): 457-463; Liu et al. (1995) Genomics 25: 674-681; Liu et al. (1993) Nucl. Acids Res. 21 (14): 3333-3334; and Zoe et al. (1999) BioTechniques 27 (2): 240-248; for RACE see, for example, PCR Protocols: A Guide to Methods and Applications, (1990) Academic Press, Inc.

[0142](2) Chemical Synthesis

[0143]In addition, the promoters and promoter control elements described in the Sequence Listing can be chemically synthesized according to techniques in common use. See, for example, Beaucage et al. (1981) Tet. Lett. 22: 1859 and U.S. Pat. No. 4,668,777.

[0144]Such chemical oligonucleotide synthesis can be carried out using commercially available devices, such as Biosearch 4600 or 8600 DNA synthesizer by Applied Biosystems, a division of Perkin-Elmer Corp. (Foster City, Calif., USA) and Expedite by Perceptive Biosystems (Framingham, Mass., USA).

[0145]Synthetic RNA, including natural and/or analog building blocks, can be synthesized on the Biosearch 8600 machines (see above).

[0146]Oligonucleotides can be synthesized and then ligated together to construct the desired polynucleotide.

B. Isolating Related Promoter Sequences

[0147]Included in the present invention are promoter and promoter control elements that are related to those described in the Sequence Listing. Such related sequences can be isolated using [0148](a) nucleotide sequence identity, [0149](b) coding sequence identity or [0150](c) common function or gene products.Relatives can include both naturally occurring promoters and non-natural promoter sequences. Non-natural related promoters include nucleotide substitutions, insertions or deletions of naturally-occurring promoter sequences that do not substantially affect transcription modulation activity. For example, the binding of relevant DNA binding proteins can still occur with the non-natural promoter sequences and promoter control elements of the present invention.

[0151]According to current knowledge, promoter sequences and promoter control elements exist as functionally important regions, such as protein binding sites and spacer regions. These spacer regions are apparently required for proper positioning of the protein binding sites. Thus, nucleotide substitutions, insertions and deletions can be tolerated in the spacer regions to a certain degree without loss of function.

[0152]In contrast, less variation is permissible in the functionally important regions since changes in the sequence can interfere with protein binding. Nonetheless, some variation in the functionally important regions is permissible so long as function is conserved.

[0153]The effects of substitutions, insertions and deletions to the promoter sequences or promoter control elements may be to increase or decrease the binding of relevant DNA binding proteins to modulate transcript levels of a polynucleotide to be transcribed. Effects may include tissue-specific or condition-specific modulation of transcript levels of the polypeptide to be transcribed. Polynucleotides representing changes to the nucleotide sequence of the DNA-protein contact region by insertion of additional nucleotides, changes to identity of relevant nucleotides, including use of chemically-modified bases, or deletion of one or more nucleotides are considered encompassed by the present invention.

[0154](1) Relatives Based on Nucleotide Sequence Identity

[0155]Included in the present invention are promoters exhibiting nucleotide sequence identity to those described in the Sequence Listing.

[0156]Definition

[0157]Typically, such related promoters exhibit at least 80% sequence identity, preferably at least 85%, more preferably at least 90%, and most preferably at least 95%, even more preferably, at least 96%, 97%, 98% or 99% sequence identity compared to those shown in the Sequence Listing. Such sequence identity can be calculated by the algorithms and computers programs described above.

[0158]Usually, such sequence identity is exhibited in an alignment region that is at least 75% of the length of a sequence shown in the Sequence Listing or corresponding full-length sequence; more usually at least 80%; more usually, at least 85%, more usually at least 90%, and most usually at least 95%, yet even more usually, at least 96%, 97%, 98% or 99% of the length of a sequence shown in the Sequence Listing.

[0159]The percentage of the alignment length is calculated by counting the number of bases of the sequence in the region of strongest alignment, e.g. a continuous region of the sequence that contains the greatest number of bases that are identical to the bases between two sequences that are being aligned. The number of bases in the region of strongest alignment is divided by the total base length of a sequence in the Sequence Listing.

[0160]These related promoters generally exhibit similar preferential transcription as those promoters described in the Sequence Listing and as described in the "observed expression pattern" and "expected expression pattern" fields of the reports of Table 1.

[0161]Construction of Polynucleotides

[0162]Naturally occurring promoters that exhibit nucleotide sequence identity to those shown in the Sequence Listing can be isolated using the techniques as described above. More specifically, such related promoters can be identified, for example, with typical hybridization procedures such as Southern blots or probing of polynucleotide libraries using varying stringencies (see above).

[0163]Non-natural promoter variants of those shown in the Sequence Listing can be constructed using cloning methods that incorporate the desired nucleotide variation. For example see Ho et al. (1989) Gene 77:51-59, which describes a site directed mutagenesis procedure using PCR.

[0164]Any related promoter showing sequence identity to those shown in the Sequence Listing can be chemically synthesized as described above.

[0165]Also, the present invention includes non-natural promoters that exhibit the above-sequence identity to those in the Sequence Listing.

[0166]The promoters and promoter control elements of the present invention may also be synthesized with 5' or 3' extensions to facilitate additional manipulation, for instance.

[0167]Testing of Polynucleotides

[0168]Polynucleotides of the invention were tested for activity by cloning the sequence into an appropriate vector, transforming plants with the construct and assaying for marker gene expression. Recombinant DNA constructs were prepared which comprise the polynucleotide sequences of the invention inserted into a vector suitable for transformation of plant cells. The construct can be made using standard recombinant DNA techniques (Sambrook et al. 1989) and can be introduced to the species of interest by Agrobacterium-mediated transformation or by other means of transformation as referenced below.

[0169]The vector backbone can be any of those typical in the art such as plasmids, viruses, artificial chromosomes, BACs, YACs and PACs and vectors of the sort described by [0170](a) BAC: Shizuya et al. (1992) Proc. Natl. Acad. Sci. USA 89: 8794-8797; Hamilton et al. (1996) Proc. Natl. Acad. Sci. USA 93: 9975-9979; [0171](b) YAC: Burke et al. (1987) Science 236:806-812; [0172](c) PAC: Sternberg N. et al. (1990) Proc Natl Acad Sci USA. 87 (1):103-7; [0173](d) Bacteria-Yeast Shuttle Vectors: Bradshaw et al. (1995) Nucl Acids Res 23: 4850-4856; [0174](e) Lambda Phage Vectors: Replacement Vector, e.g., Frischauf et al. (1983) J. Mol Biol 170: 827-842; or Insertion vector, e.g., Huynh et al., In: Glover NM (ed) DNA Cloning: A practical Approach, Vol. 1 Oxford: IRL Press (1985); T-DNA gene fusion vectors: Walden et al. (1990) Mol Cell Biol 1: 175-194; and [0175](g) Plasmid vectors: Sambrook et al., infra.

[0176]Typically, the construct comprises a vector containing a sequence of the present invention operationally linked to any marker gene. The polynucleotide was identified as a promoter by the expression of the marker gene. Although many marker genes can be used, Green Fluorescent Protein (GFP) is preferred. The vector may also comprise a marker gene that confers a selectable phenotype on plant cells. The marker may encode biocide resistance, particularly antibiotic resistance, such as resistance to kanamycin, G418, bleomycin, hygromycin or herbicide resistance, such as resistance to chlorosulfuron or phosphinotricin. Vectors can also include origins of replication, scaffold attachment regions (SARs), markers, homologous sequences, introns, etc.

[0177]Promoter Control Elements of the Invention

[0178]The promoter control elements of the present invention include those that comprise a sequence shown in the Sequence Listing or fragments thereof. The size of the fragments can range from 5 bases to 10 kilobases (kb). Typically, the fragment size is no smaller than 8 bases; more typically, no smaller than 12; more typically, no smaller than 15 bases; more typically, no smaller than 20 bases; more typically, no smaller than 25 bases; even more typically, no more than 30, 35, 40 or 50 bases.

[0179]Usually, the fragment size is no larger than 5 kb bases; more usually, no larger than 2 kb; more usually, no larger than 1 kb; more usually, no larger than 800 bases; more usually, no larger than 500 bases; even more usually, no more than 250, 200, 150 or 100 bases.

[0180]Relatives Based on Nucleotide Sequence Identity

[0181]Included in the present invention are promoter control elements exhibiting nucleotide sequence identity to those described in the Sequence Listing or fragments thereof.

[0182]Typically, such related promoters exhibit at least 80% sequence identity, preferably at least 85%, more preferably at least 90%, and most preferably at least 95%, even more preferably, at least 96%, 97%, 98% or 99% sequence identity compared to those shown in the Sequence Listing. Such sequence identity can be calculated by the algorithms and computers programs described above.

[0183]Promoter Control Element Configuration

[0184]A common configuration of the promoter control elements in RNA polymerase II promoters is described in "Models for prediction and recognition of eukaryotic promoters", T. Werner (1999) Mammalian Genome 10, 168-175.

[0185]Promoters are generally modular in nature. Promoters can consist of a basal promoter which functions as a site for assembly of a transcription complex comprising an RNA polymerase, for example RNA polymerase II. A typical transcription complex will include additional factors such as TF_IIB, TF_IID and TF_IIE. Of these, TF_IID appears to be the only one to bind DNA directly. The promoter might also contain one or more promoter control elements such as the elements discussed above. These additional control elements may function as binding sites for additional transcription factors that have the function of modulating the level of transcription with respect to tissue specificity, of transcriptional responses to particular environmental or nutritional factors and the like.

[0186]One type of promoter control element is a polynucleotide sequence representing a binding site for proteins. Typically, within a particular functional module, protein binding sites constitute regions of 5 to 60, preferably 10 to 30, more preferably 10 to 20 nucleotides. Within such binding sites, there are typically 2 to 6 nucleotides which specifically contact amino acids of the nucleic acid binding protein.

[0187]The protein binding sites are usually separated from each other by 10 to several hundred nucleotides, typically by 15 to 150 nucleotides, often by 20 to 50 nucleotides.

[0188]Further, protein binding sites in promoter control elements often display dyad symmetry in their sequence. Such elements can bind several different proteins and/or a plurality of sites can bind the same protein. Both types of elements may be combined in a region of 50 to 1,000 base pairs.

[0189]Binding sites for any specific factor have been known to occur almost anywhere in a promoter. For example, functional AP-1 binding sites can be located far upstream, as in the rat bone sialoprotein gene where an AP-1 site located about 900 nucleotides upstream of the transcription start site suppresses expression (Yamauchi et al. (1996) Matrix Biol. 15, 119-130). Alternatively, an AP-1 site located close to the transcription start site plays an important role in the expression of Moloney murine leukemia virus (Sap et al. (1989) Nature 340, 242-244.

[0190](2) Those Identifiable by Bioinformatics

[0191]Promoter control elements from the promoters of the instant invention can be identified utilizing bioinformatic or computer driven techniques.

[0192]One method uses a computer program AlignACE to identify regulatory motifs in genes that exhibit common preferential transcription across a number of time points. The program identifies common sequence motifs in such genes. See, Roth et al. (1998) Nature Biotechnol. 16: 949-945; Tavazoie et al. (1999) Nat Genet Jul; 22 (3):281-5;

[0193]Genomatix also makes available a GEMS Launcher program and other programs to identify promoter control elements and configuration of such elements. Genomatix is located in Munich, Germany.

[0194]Other references also describe detection of promoter modules by models independent of overall nucleotide sequence similarity. See, for instance, Klingenhoff et al. (1999) Bioinformatics 15: 180-186.

[0195]Protein binding sites of promoters can be identified as reported in "Computer-assisted prediction, classification, and delimination of protein binding sites in nucleic acids", Frech et al. (1993) Nucleic Acids Research 21 (7): 1655-1664.

[0196]Other programs used to identify protein binding sites include, for example, Signal Scan (Prestridge et al. (1996) Comput. Appl. Biosci. 12: 157-160); Matrix Search (Chen et al. (1995) Comput. Appl. Biosci. 11: 563-566), available as part of Signal Scan 4.0; MatInspector (Ghosh et al. (1993) Nucl. Acid Res. 21: 3117-3118) available via the internet; ConsInspector (Frech et al. (1993) Nucl. Acids Res. 21: 1655-1664), available via the internet; TFSearch and TESS.

[0197]Frech et al. (1997) "Software for the analysis of DNA sequence elements of transcription", Bioinformatics & Sequence Analysis, Vol. 13, no. 1, 89-97 is a review of different software for analysis of promoter control elements. This paper also reports the usefulness of matrix-based approaches to yield more specific results.

[0198]For other procedures, see Fickett et al. (2000) Curr. Op. Biotechnol. 11: 19-24 and Quandt et al. (1995) Nucleic Acids Res. 23: 4878-4884.

[0199](3) Those Identifiable by In-Vitro and In-Vivo Assays

[0200]Promoter control elements can also be identified with in-vitro assays such as transcription detection methods and with in-vivo assays such as enhancer trapping protocols.

[0201]In-Vitro Assays

[0202]Examples of in-vitro assays include detection of binding of protein factors that bind promoter control elements. Fragments of the instant promoters can be used to identify the location of promoter control elements. Another option for obtaining a promoter control element with desired properties is to modify known promoter sequences. This is based on the fact that the function of a promoter is dependent on the interplay of regulatory proteins that bind to specific, discrete nucleotide sequences ("motifs") in the promoter. Such interplay subsequently affects the general transcription machinery and regulates transcription efficiency. These regulatory proteins are positive regulators or negative regulators (repressors) and one protein can have a dual role depending on the context (Johnson and McKnight, (1989) Annu. Rev. Biochem. 58:799-839).

[0203]One type of in-vitro assay uses a known DNA binding factor to isolate DNA fragments that bind. If a fragment or promoter variant does not bind, then a promoter control element has been removed or disrupted. For specific assays, see, for instance, Luo et al. (1997) J. Mol. Biol. 266:470, Chusacultanachai et al. (1999) J. Biol. Chem. 274:23591, Fabbro et al. (1995) Biochem. Biophys. Res. Comm. 213:781).

[0204]Alternatively, a DNA fragment suspected of conferring a particular pattern of expression can be examined for the ability to bind transcription factors responsible for generating the particular pattern using methods such as DNA footprinting (e.g. Cousins et al. (2000) Immunology 99:101 and V. Kolla et al. (1999) Biochem. Biophys. Res. Comm. 266:5) or "mobility-shift" assays (Fabiani et al. (2000) J. Biochem. 347:147 and Sugiura et al. (2000) J. Biochem 347:155) or fluorescence polarization (e.g. Royer et al. U.S. Pat. No. 5,445,935). Both mobility shift and DNA footprinting assays can also be used to identify portions of large DNA fragments that are bound by proteins in unpurified transcription extracts prepared from tissues or organs of interest.

[0205]Cell-free transcription extracts can be prepared and used to directly assay in a reconstitutable system (Narayan et al. (2000) Biochemistry 39:818).

[0206]In-Vivo Assays

[0207]Promoter control elements can be identified with reporter genes in in-vivo assays with the use of fragments of the instant promoter, polynucleotides or variants thereof. That is, a fragment(s) comprising a basal or "core" promoter operably linked to a reporter sequence can be inserted into a vector. When transcribed, a detectable label is produced. Examples of reporter genes include those encoding luciferase, green fluorescent protein, GUS, neo, cat and bar. Alternatively, the transcribed reporter sequence can be detected with AFLP and microarray techniques.

[0208]In promoter probe vector systems, genomic DNA fragments are inserted upstream of the coding sequence of a reporter gene which is expressed only when the cloned fragment contains DNA having transcription modulation activity (Neveet al. (1979) Nature 277:324-325). No transcription occurs when control elements are present in the fragment or when control elements present are disrupted. Probe vectors have been designed for assaying transcription modulation in E. coli (An et al. (1979) J. Bact. 140:400-407) and other bacterial hosts (Band et al. (1983) Gene 26:313-315 and Achen (1986) Gene 45:45-49), yeast (Goodey et al. (1986) Mol. Gen. Genet. 204:505-511) and mammalian cells (Pater et al. (1984) J. Mol. App. Gen. 2:363-371).

[0209]A different design of a promoter/control element trap includes packaging into retroviruses for more efficient delivery into cells. One type of retroviral enhancer trap was described by von Melchner et al. (Genes Dev. (1992); U.S. Pat. No. 5,364,783). The basic design of this vector includes a reporter protein coding sequence engineered into the U3 portion of the 3' LTR. No splice acceptor consensus sequences are included, limiting its utility to work as an enhancer trap only. A different approach to a gene trap using retroviral vectors was pursued by Friedrich and Soriano (Genes Dev. 1991) who engineered a lacZ-neo fusion protein linked to a splicing acceptor. LacZ-neo fusion protein expression from trapped loci allows not only for drug selection, but also for visualization of 13-galatactosidase expression using the chromogenic substrate, X-gal.

[0210]A general review of tools for identifying transcriptional regulatory regions of genomic DNA is provided by J. W. Fickett et al. (Curr. Opn. Biotechnol (2000) 11:19).

[0211](4) Non-Natural Control Elements

[0212]Non-natural control elements can be constructed by inserting, deleting or substituting nucleotides into the promoter control elements described above. Such control elements are capable of transcription modulation that can be determined using any of the assays described above.

C. Constructing Promoters with Control Elements

[0213](1) Combining Promoters and Promoter Control Elements

[0214]The promoter polynucleotides and promoter control elements of the present invention, both naturally occurring and synthetic, can be combined with each other to produce the desired preferential transcription. In addition, the polynucleotides of the invention can be combined with other known sequences to generate promoters useful for modulating, for example, tissue-specific transcription or condition-specific transcription. Such preferential transcription can be determined using the techniques or assays described above.

[0215]The relatives, fragments and variants as well as full-length sequences shown in the Sequence Listing are useful alone or in combination.

[0216]The location and relation of promoter control elements within a promoter can affect the ability of the promoter to modulate transcription. The order and spacing of control elements is a factor when constructing promoters.

[0217](2) Number of Promoter Control Elements

[0218]Promoters can contain any number of control elements. For example, a promoter can contain multiple transcription binding sites or other control elements. One element may confer tissue or organ specificity, another element may limit transcription to specific time periods, etc. Typically, promoters will contain at least a basal or core promoter as described above. Any additional element can be included as desired. For example, a fragment comprising a basal or "core" promoter can be fused with another fragment with any number of additional control elements.

[0219](3) Spacing Between Control Elements

[0220]Spacing between control elements or the configuration or control elements can be determined or optimized to permit the desired polynucleotide or protein-polynucleotide interactions to occur.

[0221]For example, if two transcription factors bind to a promoter simultaneously or relatively close in time, the binding sites are spaced to allow each factor to bind without steric hinderance. The spacing between two such hybridizing control elements can be as small as a profile of a protein bound to a control element. In some cases, two protein binding sites can be adjacent to each other when the proteins bind at different times during the transcription process.

[0222]Further, when two control elements hybridize the spacing between such elements will be sufficient to allow the promoter polynucleotide to form a hairpin or loop so as to permit the two elements to bind. The spacing between two such hybridizing control elements can be as small as a t-RNA loop, to as large as 10 kb.

[0223]Typically, the spacing is no smaller than 5 bases, more typically no smaller than 8, more typically no smaller than 15 bases, more typically no smaller than 20 bases, more typically no smaller than 25 bases, even more typically no more than 30, 35, 40 or 50 bases.

[0224]Usually, the fragment size in no larger than 5 kb bases, more usually no larger than 2 kb, more usually no larger than 1 kb, more usually no larger than 800 bases, more usually no larger than 500 bases, even more usually no more than 250, 200, 150 or 100 bases.

[0225]Such spacing between promoter control elements can be determined using the techniques and assays described above.

[0226](4) Other Promoters

[0227]The following are promoters that are induced under stress conditions and can be combined with those of the present invention: 1dh1 (oxygen stress, tomato see Germain and Ricard (1997) Plant Mol Biol 35:949-54), GPx and CAT (oxygen stress, mouse, see Franco et al. (1999) Free Radic Biol Med 27:1122-32), ci7 (cold stress, potato, see Kirch et al. (1997) Plant Mol Biol. 33:897-909), Bz2 (heavy metals, maize, see Mans and Walbot (1997) Plant Physiol 113:93-102), HSP32 (hyperthermia, rat, see Raju and Maines (1994) Biochim Biophys Acta 1217:273-80); MAPKAPK-2 (heat shock, Drosophila, see Larochelle and Suter (1995) Gene 163:209-14).

[0228]In addition, the following promoters are examples those induced by the presence or absence of light and can be used in combination with those of the present invention: Topoisomerase II (pea, see Reddy et al. (1999) Plant Mol Biol 41:125-37), chalcone synthase (soybean, see Wingender et al. (1989) Mol Gen Genet 218:315-22), mdm2 gene (human tumor, see Saucedo et al. (1998) Cell Growth Differ 9:119-30), Clock and BMAL1 (rat, see Namihira et al. (1999) Neurosci Lett 271:1-4), PHYA (Arabidopsis, see Canton and Quail 1999 Plant Physiol 121:1207-16), PRB-1b (tobacco, see Sessa et al. (1995) Plant Mol Biol 28:537-47) and Ypr10 (common bean, see Walter et al. (1996) Eur J Biochem 239:281-93).

[0229]The promoters and control elements of the following genes can be used in combination with the present invention to confer tissue specificity: for roots MipB (iceplant, Yamada et al. (1995) Plant Cell 7:1129-42) and SUCS (root nodules, broadbean, Kuster et al. (1993) Mol Plant Microbe Interact 6:507-14), for leaves OsSUT1 (rice, Hirose et al. (1997) Plant Cell Physiol 38:1389-96), for siliques Msg (soybean, Stomvik et al. (1999) Plant Mol Biol 41:217-31) and for inflorescence (Arabidopsis, Shani et al. (1997) Plant Mol Biol 34 (6):837-42) and ACT11 (Arabidopsis, Huang et al. (1997) Plant Mol Biol 33:125-39).

[0230]Still other promoters are affected by hormones or participate in specific physiological processes, which can be used in combination with those of present invention. Some examples are the ACC synthase gene that is induced differently by ethylene and brassinosteroids (mung bean, Yi et al. (1999) Plant Mol Biol 41:443-54), the TAPG1 gene that is active during abscission (tomato, Kalaitzis et al. (1995) Plant Mol Biol 28:647-56) and the 1-aminocyclopropane-1-carboxylate synthase gene (carnation, Jones et al. (1995) Plant Mol Biol 28:505-12) and the CP-2/cathepsin L gene (rat, Kim and Wright (1997) Biol Reprod 57:1467-77), which are both active during senescence.

E. Vectors

[0231]Vectors are a useful component of the present invention. In particular, vectors can deliver the present promoters and/or promoter control elements to a cell. For the purposes of this invention, such delivery ranges from randomly introducing the promoter or promoter control element alone into a cell to integrating the vector containing the promoter or promoter control element into a cell's genome. Thus, a vector need not be limited to a DNA molecule such as a plasmid, cosmid or bacterial phage that has the capability of replicating autonomously in a host cell. All other manner of delivery of the promoters and promoter control elements of the invention are envisioned. The various T-DNA vector types are preferred vectors for use with the present invention. Many useful vectors are commercially available.

[0232]It may also be useful to attach a marker sequence to the present promoter and promoter control element in order to determine activity of such sequences. Marker sequences typically include genes that provide antibiotic resistance, such as tetracycline resistance, hygromycin resistance or ampicillin resistance, or provide herbicide resistance. Specific selectable marker genes may be used to confer resistance to herbicides such as glyphosate, glufosinate or broxynil (Comai et al. (1985) Nature 317: 741-744; Gordon-Kamm et al. (1990) Plant Cell 2: 603-618; and Stalker et al. (1988) Science 242: 419-423). Other marker genes exist which provide hormone responsiveness.

[0233](1) Modification of Transcription by Promoters and Promoter Control Elements

[0234]The promoter or promoter control element of the present invention may be operably linked to a polynucleotide to be transcribed. In this manner, the promoter or promoter control element modifys transcription by modulating transcript levels of that polynucleotide when inserted into a genome.

[0235]The promoter or promoter control element need not be linked, operably or otherwise, to a polynucleotide to be transcribed before being inserted into a genome. For example, the promoter or promoter control element can be inserted into the genome in front of a polynucleotide already present therein. Here, the promoter or promoter control element modulates the transcription of a polynucleotide that was already present in the genome. This polynucleotide may be native to the genome or inserted at an earlier time.

[0236]Alternatively, the promoter or promoter control element can simply be inserted into a genome or maintained extrachromosomally as a way to divert the transcription resources of the system to itself. See, for example, Vaucheret et al. (1998) Plant J 16: 651-659. This approach may be used to downregulate the transcript levels of a group of polynucleotide(s).

[0237](2) Polynucleotide to be Transcribed

[0238]The nature of the polynucleotide to be transcribed is not limited. Specifically, the polynucleotide may include sequences that will have activity as RNA as well as sequences that result in a polypeptide product. These sequences may include, but are not limited to antisense sequences, ribozyme sequences, spliceosomes, amino acid coding sequences and fragments thereof.

[0239]Specific coding sequences may include, but are not limited to endogenous proteins or fragments thereof, or heterologous proteins including marker genes or fragments thereof.

[0240]Promoters and control elements of the present invention are useful for modulating metabolic or catabolic processes. Such processes include, but are not limited to secondary product metabolism, amino acid synthesis, seed protein storage, oil development, pest defense and nitrogen usage. Some examples of genes, transcripts, peptides or polypeptides participating in these processes which can be modulated by the present invention: are tryptophan decarboxylase (tdc), strictosidine synthase (str1), dihydrodipicolinate synthase (DHDPS), aspartate kinase (AK), 2S albumin, alpha-, beta-, and gamma-zeins, ricinoleate, 3-ketoacyl-ACP synthase (KAS), Bacillus thuringiensis (Bt) insecticidal protein, cowpea trypsin inhibitor (CpTI), asparagine synthetase and nitrite reductase. Alternatively, expression constructs can be used to inhibit expression of these peptides and polypeptides by incorporating the promoters in constructs for antisense use, co-suppression use or for the production of dominant negative mutations.

[0241](3) Other Regulatory Elements

[0242]As explained above, several types of regulatory elements exist concerning transcription regulation. Each of these regulatory elements may be combined with the present vector if desired.

[0243](4) Other Components of Vectors

[0244]Translation of eukaryotic mRNA is often initiated at the codon that encodes the first methionine. Thus, when constructing a recombinant polynucleotide for expressing a protein product according to the present invention, it is preferable to ensure that no intervening codons encoding a methionine are contained within the linkage between the polynucleotide to be transcribed, or a functional derivative thereof, and the 3' portion of the promoter, preferably including the TATA box.

[0245]The vector of the present invention may contain additional components. For example, an origin of replication that allows for replication of the vector in a host cell may be added. In addition, homologous sequences flanking a target location in the genome may be added to allow for site-specific recombination of a specific sequence contained in the vector. T-DNA sequences also allow for insertion of a specific sequence randomly into a target genome, but in a random manner.

[0246]The vector may also contain a plurality of restriction sites for insertion of the promoter and/or promoter control elements of the present invention as well as any polynucleotide to be transcribed. The vector can additionally contain selectable marker genes. The vector can also contain a transcriptional and translational initiation region and/or a transcriptional and translational termination region that functions in the host cell. The termination region may be native with the transcriptional initiation region, may be native with the polynucleotide to be transcribed or may be derived from another source. Convenient termination regions are available from the Ti-plasmid of A. tumefaciens, such as the octopine synthase and nopaline synthase termination regions. See also, Guerineau et al. (1991) Mol. Gen. Genet. 262:141-144; Proudfoot (1991) Cell 64:671-674; Sanfacon et al. (1991) Genes Dev. 5:141-149; Mogen et al. (1990) Plant Cell 2:1261-1272; Munroe et al. (1990) Gene 91:151-158; Ballas et al. (1989) Nucleic Acids Res. 17:7891-7903; Joshi et al. (1987) Nucleic Acid Res. 15:9627-9639.

[0247]Where appropriate, the polynucleotide to be transcribed may be optimized for increased expression in a certain host cell. For example, the polynucleotide can be synthesized using preferred codons for improved transcription and translation. See U.S. Pat. Nos. 5,380,831, 5,436, 391 and Murray et al. (1989) Nucleic Acids Res. 17:477-498.

[0248]Additional sequence modifications include elimination of sequences encoding spurious polyadenylation signals, exon intron splice site signals, transposon-like repeats and other such sequences well characterized as deleterious to expression. The G-C content of the polynucleotide may be adjusted to the average levels for a given cellular host, as calculated by reference to known genes expressed in the host cell. The polynucleotide sequence may be modified to avoid hairpin secondary mRNA structures.

[0249]A general description of expression vectors and reporter genes can be found in Gruber et al., "Vectors for Plant Transformation", in Methods in Plant Molecular Biology & Biotechnology (1993) Glich et al. eds., pp. 89-119, CRC Press). Moreover GUS expression vectors and GUS gene cassettes are available from Clonetech Laboratories, Inc. (Palo Alto, Calif.) while luciferase expression vectors and luciferase gene cassettes are available from Promega Corp. (Madison, Wis.). GFP vectors are available from Aurora Biosciences.

F. Polynucleotide Insertion Into a Host Cell

[0250]The polynucleotides according to the present invention can be inserted into a host cell. A host cell includes but is not limited to a plant, mammalian, insect, yeast and prokaryotic cell, preferably a plant cell.

[0251]The method of insertion into the host cell genome is chosen based on convenience. For example, the insertion into the host cell genome may either be accomplished by vectors that integrate into the host cell genome or by vectors which exist independent of the host cell genome.

[0252](1) Polynucleotides Autonomous of the Host Genome

[0253]The polynucleotides of the present invention can exist autonomously or independent of the host cell genome. Vectors of these types are known in the art and include, for example, certain types of non-integrating viral vectors, autonomously replicating plasmids, artificial chromosomes and the like.

[0254]Additionally, in some cases transient expression of a polynucleotide may be desired.

[0255](2) Polynucleotides Integrated into the Host Genome

[0256]The promoter sequences, promoter control elements or vectors of the present invention can be transformed into host cells. These transformations can be into protoplasts or isolated cells or intact tissues. Preferably, expression vectors are introduced into intact tissue. General methods of culturing plant tissues are provided for example by Maki et al. ("Procedures for Introducing Foreign DNA into Plants" in Methods in Plant Molecular Biology & Biotechnology (1993) Glich et al. eds., pp. 67-88 CRC Press) and by Phillips et al. "Cell-Tissue Culture and In-Vitro Manipulation" in Corn & Corn Improvement, 3rd Edition 10Sprague et al. (1998) eds. pp. 345-387) American Society of Agronomy Inc. et al.

[0257]Methods of introducing polynucleotides into plant tissue include the direct infection or co-cultivation of a plant cell with Agrobacterium tumefaciens (Horsch et al. (1985) Science 227:1229). Descriptions of Agrobacterium vector systems and methods for Agrobacterium-mediated gene transfer are provided by Gruber et al. supra.

[0258]Alternatively, polynucleotides are introduced into plant cells or other plant tissues using a direct gene transfer method such as microprojectile-mediated delivery, DNA injection, electroporation and the like. More preferably, polynucleotides are introduced into plant tissues using the microprojectile media delivery with the biolistic device. See, for example, Tomes et al., "Direct DNA transfer into intact plant cells via microprojectile bombardment" In: Plant Cell, Tissue and Organ Culture: Fundamental Methods (1995) Gamborg and Phillips eds. Springer Verlag, Berlin.

[0259]In another embodiment of the current invention, expression constructs can be used for gene expression in callus culture for the purpose of expressing marker genes encoding peptides or polypeptides that allow identification of transformed plants. Here, a promoter that is operatively linked to a polynucleotide to be transcribed is transformed into plant cells and the transformed tissue is then placed on callus-inducing media. If the transformation is conducted with leaf discs, for example, callus will initiate along the cut edges. Once callus growth has initiated, callus cells can be transferred to callus shoot-inducing or callus root-inducing media. Gene expression will occur in the callus cells developing on the appropriate media: callus root-inducing promoters will be activated on callus root-inducing media, etc. Examples of such peptides or polypeptides useful as transformation markers include, but are not limited to barstar, glyphosate, chloramphenicol acetyltransferase (CAT), kanamycin, spectinomycin, streptomycin or other antibiotic resistance enzymes, green fluorescent protein (GFP) and β-glucuronidase (GUS), etc. Some of the promoters in the Sequence Listing will also be capable of sustaining expression in some tissues or organs after the initiation or completion of regeneration. Examples of these tissues or organs are somatic embryos, cotyledon, hypocotyl, epicotyl, leaf, stems, roots, flowers and seed.

[0260]Integration into the host cell genome also can be accomplished by methods known in the art such as by homologous sequences or T-DNA discussed above or by using the cre-lox system (Vergunst et al. (1998) Plant Mol. Biol. 38:393).

G. Utility

[0261]Common Uses

[0262]In yet another embodiment, the promoters of the present invention can be used to further understand developmental mechanisms. For example, promoters that are specifically induced during callus formation, somatic embryo formation, shoot formation or root formation can be used to explore the effects of overexpression, repression or ectopic expression of target genes, or for isolation of trans-acting factors.

[0263]The vectors of the invention can be used not only for expression of coding regions but may also be used in exon-trap cloning, or promoter trap procedures to detect differential gene expression in various tissues. See Lindsey et al (1993) "Tagging Genomic Sequences That Direct Transgene Expression by Activation of a Promoter Trap in Plants," Transgenic Research 2:3347 and Auch et al. "Exon Trap Cloning: Using PCR to Rapidly Detect and Clone Exons from Genomic DNA Fragments," Nucleic Acids Research, 18:674.

[0264]Entrapment vectors, first described for use in bacteria (Casadaban and Cohen (1979) Proc. Nat. Aca. Sci. U.S.A. 76: 4530 and Casadaban et al. (1980) J. Bacteriol. 143: 971) permit selection of insertional events that lie within coding sequences. Entrapment vectors can be introduced into pluripotent ES cells in culture and then passed into the germline via chimeras (Gossler et al. (1989) Science 244: 463 and Skarnes (1990) Biotechnology 8: 827). Promoter or gene trap vectors often contain a reporter gene, e.g. lacZ, lacking its own promoter and/or splice acceptor sequence upstream. That is, promoter gene traps contain a reporter gene with a splice site but no promoter. If the vector lands in a gene and is spliced into the gene product, then the reporter gene is expressed.

[0265]Recently, the isolation of preferentially-induced genes has been made possible with the use of sophisticated promoter traps (e.g. IVET) that are based on conditional auxotrophy complementation or drug resistance. In one IVET approach, various bacterial genome fragments are placed in front of a necessary metabolic gene that is coupled to a reporter gene. The DNA constructs are inserted into a bacterial strain otherwise lacking the metabolic gene and the resulting bacteria are used to infect the host organism. Only bacteria expressing the metabolic gene survive in the host organism. Consequently, inactive constructs can be eliminated by harvesting only bacteria that survive for some minimum period in the host. At the same time, constitutively active constructs can be eliminated by selecting only bacteria that do not express the reporter gene under laboratory conditions. The bacteria selected by such a method contain constructs that are selectively induced only during infection of the host. The IVET approach can be modified in plants to identify genes induced in either the bacteria or the plant cells upon pathogen infection or root colonization. For information on IVET see the following articles: Mahan et al. (1993) Science 259:686-688, Mahan et al. (1995) PNAS USA 92:669-673, Heithoff et al. (1997) PNAS USA 94:934-939, and Wang et al. (1996) PNAS USA. 93:10434.

[0266]Constitutive Transcription

[0267]Promoters and control elements providing constitutive transcription are desired for modulation of transcription in most cells of an organism under most environmental conditions. In a plant, for example, constitutive transcription is useful for modulating genes involved in defense, pest resistance, herbicide resistance, etc.

[0268]Constitutive up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase defense, pest and herbicide resistance may require constitutive up-regulation of transcription. In contrast, constitutive down-regulation of transcriptional may be desired to inhibit those genes, transcripts, and/or polypeptides that lower defense, pest and herbicide resistance.

[0269]Typically, promoter or control elements that provide constitutive transcription produce transcription levels that are statistically similar in many tissues and environmental conditions observed.

[0270]Calculation of P-value from the different observed transcript levels is one means of determining whether a promoter or control element is providing constitutive up-regulation. P-value is the probability that the difference of transcript levels is not statistically significant. The higher the P-value, the more likely the difference of transcript levels is not significant. One formula used to calculate P-value is as follows:

∫ Φ ( x ) x , integrated from a to ∞ , where Φ ( x ) is a normal distribution ; ##EQU00001## where a = Sx - μ σ ( all Samples except Sx ) ; ##EQU00001.2## where Sx = the intensity of the sample of interest ##EQU00001.3## where μ = is the average of the intensities of all samples except Sx , = ( Σ S 1 Sn ) - Sx n - 1 ##EQU00001.4##

[0271]where σ (S1 . . . S11, not including Sx)=the standard deviation of all sample intensities except Sx.

The P-value from the formula ranges from 1.0 to 0.0.

[0272]Usually, each P-value of the transcript levels produced by the promoter or control element and observed in a majority of cells, tissues or organs under various environmental conditions is greater than 10^-8; more usually, greater than 10^-7; even more usually, greater than 10^-6; even more usually, greater than 10^-5 or 10^-4.

[0273]For up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0274]Stress Induced Preferential Transcription

[0275]Promoters and control elements providing modulation of transcription under oxidative, drought, oxygen, wound and methyl jasmonate stress are particularly useful for producing host cells or organisms that are more resistant to biotic and abiotic stresses. For example, in a plant modulation of genes, transcripts and/or polypeptides in response to oxidative stress can protect cells against damage caused by oxidative agents such as hydrogen peroxide and other free radicals.

[0276]Drought induction of genes, transcripts and/or polypeptides are useful to increase the viability of a plant, for example when water is a limiting factor. In contrast, genes, transcripts and/or polypeptides induced during oxygen stress can help the flood tolerance of a plant.

[0277]The promoters and control elements of the present invention can modulate the plant's response to stresses. Examples of some genes involved in stress condition responses are VuPLD1 (drought stress, Cowpea; Pham-Thi et al. (1999) Plant Mol. Biol 1257-65), pyruvate decarboxylase (oxygen stress, rice; Rivosal et al. (1997) Plant Physiol. 114 (3): 1021-29), and the chromoplast specific carotenoid gene (oxidative stress, Capsicum; see Bouvier et al. (1998) J Biol Chem 273: 30651-59).

[0278]Promoters and control elements providing preferential transcription during wounding or that are induced by methyl jasmonate can produce a defense response in host cells or organisms. In a plan, preferential modulation of genes, transcripts and/or polypeptides under such conditions is useful to induce a defense response to mechanical wounding, pest or pathogen attack or treatment with certain chemicals.

[0279]Promoters and control elements of the present invention also can trigger a response similar to those described for cf9 (viral pathogen, tomato; O'Donnell et al. (1998) Plant J 14 (1): 137-42), hepatocyte growth factor activator inhibitor type 1 (HAI-1), which enhances tissue regeneration (tissue injury, human; Koono et al. (1999) J Histochem Cytochem 47: 673-82), copper amine oxidase (CuAO) induced during ontogenesis and wound healing (wounding, chick-pea; Rea et al. (1998) FEBS Letters 437: 177-82), proteinase inhibitor II (wounding, potato; Pena-Cortes et al. (1988) Planta 174: 84-89), protease inhibitor II (methyl jasmonate, tomato; Farmer and Ryan (1990) Proc Natl Acad Sci USA 87: 7713-7716) and two vegetative storage protein genes VspA and VspB (wounding, jasmonic acid and water deficit; soybean; Mason and Mullet (1990) Plant Cell 2: 569-579).

[0280]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase oxidative, flood or drought tolerance may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit those genes, transcripts and/or polypeptides that lower such tolerance.

[0281]Typically, promoter or control elements which provide preferential transcription in wounding or under methyl jasmonate induction produce transcript levels that are statistically significantly altered as compared to cell types, organs or tissues under other conditions.

[0282]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0283]Light Induced Preferential Transcription

[0284]Promoters and control elements providing preferential transcription when induced by light exposure can be utilized to modulate growth, metabolism and development to increase drought tolerance and to decrease damage from light stress for host cells or organisms. In a plant, modulation of genes, transcripts and/or polypeptides in response to light is useful [0285](1) to increase the photosynthetic rate; [0286](2) to increase storage of certain molecules in leaves or green parts only, e.g. silage with high protein or starch content; [0287](3) to modulate production of exogenous compositions in green tissue, e.g. certain feed enzymes; [0288](4) to induce growth or development, such as fruit development and maturity, during extended exposure to light; [0289](5) to modulate guard cells to control the size of stomata in leaves to prevent water loss, or [0290](6) to induce accumulation of beta-carotene to help plants cope with light induced stress.The promoters and control elements of the present invention can also trigger responses similar to those described for: abscisic acid insensitive3 (ABI3) (dark-grown Arabidopsis seedlings, Rohde et al. (2000) Plant Cell 12: 35-52), asparagine synthetase (pea root nodules, Tsai, Coruzzi, (1990) EMBO J 9: 323-32) and mdm2 gene (human tumor; Saucedo et al. (1998) Cell Growth Differ 9: 119-30).

[0291]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase drought or light tolerance may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit those genes, transcripts and/or polypeptides that lower such tolerance.

[0292]Typically, promoter or control elements which provide preferential transcription in cells, tissues or organs exposed to light produce transcript levels that are statistically significantly altered as compared to cells, tissues or organs under decreased light exposure (intensity or length of time).

[0293]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0294]Dark Induced Preferential Transcription

[0295]Promoters and control elements providing preferential transcription when induced by dark or decreased light intensity or decreased light exposure time can be utilized to time growth, metabolism and development and to modulate photosynthesis capabilities for host cells or organisms. In a plant, modulation of genes, transcripts and/or polypeptides in response to dark is useful [0296](1) to induce growth or development, such as fruit development and maturity, despite lack of light; [0297](2) to modulate genes, transcripts and/or polypeptide active at night or on cloudy days; or [0298](3) to preserve the plastid ultra structure present at the onset of darkness.The present promoters and control elements can also trigger response similar to those described in the section above.

[0299]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth and development may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit those genes, transcripts and/or polypeptides that modulate photosynthesis capabilities.

[0300]Typically, promoter or control elements which provide preferential transcription under exposure to dark or decreased light intensity or decreased exposure time produce transcript levels that are statistically significantly altered.

[0301]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0302]Leaf Preferential Transcription

[0303]Promoters and control elements providing preferential transcription in a leaf can modulate growth, metabolism and development or modulate energy and nutrient utilization in host cells or organisms. In a plant, preferential modulation of genes, transcripts and/or polypeptide in a leaf is useful [0304](1) to modulate leaf size, shape, and development; [0305](2) to modulate the number of leaves; or [0306](3) to modulate energy or nutrient usage in relation to other organs and tissues.

[0307]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit energy usage in a leaf and to redirect it to the fruit instead, for instance.

[0308]Typically, promoter or control elements which provide preferential transcription in the cells, tissues, or organs of a leaf produce transcript levels that are statistically significantly altered as compared to other cells, organs or tissues.

[0309]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0310]Root Preferential Transcription

[0311]Promoters and control elements providing preferential transcription in a root can modulate growth, metabolism, development, nutrient uptake, nitrogen fixation or modulate energy and nutrient utilization in host cells or organisms. In a plant, for example, preferential modulation of genes, transcripts, and/or in a leaf, is useful [0312](1) to modulate root size, shape, and development; [0313](2) to modulate the number of roots, or root hairs; [0314](3) to modulate mineral, fertilizer, or water uptake; [0315](4) to modulate transport of nutrients; or [0316](4) to modulate energy or nutrient usage in relation to other organs and tissues.

[0317]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit nutrient usage in a root and to redirect it to the leaf instead, for instance.

[0318]Typically, promoter or control elements which provide preferential transcription in cells, tissues or organs of a root produce transcript levels that are statistically significantly altered as compared to other cells, organs or tissues.

[0319]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0320]Stem/Shoot Preferential Transcription

[0321]Promoters and control elements providing preferential transcription in a stem or shoot can modulate growth, metabolism and development or modulate energy and nutrient utilization in host cells or organisms. In a plant, preferential modulation of genes, transcripts and/or a polypeptide in a stem or shoot is useful [0322](1) to modulate stem/shoot size, shape, and development; or [0323](2) to modulate energy or nutrient usage in relation to other organs and tissues

[0324]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit energy usage in a stem/shoot and to redirect it to the fruit instead, for instance.

[0325]Typically, promoter or control elements which provide preferential transcription in the cells, tissues or organs of a stem or shoot produce transcript levels that are statistically significant as compared to other cells, organs or tissues.

[0326]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0327]Fruit and Seed Preferential Transcription

[0328]Promoters and control elements providing preferential transcription in a silique or fruit can time growth, development, or maturity; or modulate fertility; or modulate energy and nutrient utilization in host cells or organisms. In a plant preferential modulation of genes, transcripts and/or polypeptides in a fruit is useful [0329](1) to modulate fruit size, shape, development, and maturity; [0330](2) to modulate the number of fruit or seeds; [0331](3) to modulate seed shattering; [0332](4) to modulate components of seeds, such as, storage molecules, starch, protein, oil, vitamins, anti-nutritional components, such as phytic acid; [0333](5) to modulate seed and/or seedling vigor or viability; [0334](6) to incorporate exogenous compositions into a seed, such as lysine rich proteins; [0335](7) to permit similar fruit maturity timing for early and late blooming flowers; or [0336](8) to modulate energy or nutrient usage in relation to other organs and tissues.

[0337]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts, and/or polypeptides that increase growth may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit late fruit maturity, for instance.

[0338]Typically, promoter or control elements which provide preferential transcription in the cells, tissues or organs of siliques or fruits produce transcript levels that are statistically significantly altered as compared to other cells, organs or tissues.

[0339]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0340]Callus Preferential Transcription

[0341]Promoters and control elements providing preferential transcription in a callus can be useful to modulating transcription in dedifferentiated host cells. In a plant transformation, for example, preferential modulation of genes or transcript in callus is useful to modulate transcription of a marker gene, which can facilitate selection of cells that are transformed with exogenous polynucleotides.

[0342]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase marker gene detectability may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to increase the ability of the calluses to differentiate, for instance.

[0343]Typically, promoter or control elements which provide preferential transcription in callus produce transcript levels that are statistically significantly altered as compared to other cell types, tissues, or organs. Calculation of P-value from the different observed transcript levels is one means of determining whether a promoter or control element is providing such preferential transcription.

[0344]Usually, each P-value of the transcript levels observed in callus as compared to at least one other cell type, tissue or organ, is less than 10^-4; more usually, less than 10^-5; even more usually, less than 10^-6; even more usually, less than 10^-7 or 10^-8.

[0345]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0346]Flower Specific Transcription

[0347]Promoters and control elements providing preferential transcription in flowers can modulate pigmentation or modulate fertility in host cells or organisms. In a plant, preferential modulation of genes, transcripts and/or polypeptides in a flower is useful, [0348](1) to modulate petal color; or [0349](2) to modulate the fertility of pistil and/or stamen.

[0350]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase pigmentation may require up-regulation of transcription. In contrast, transcriptional down-regulation maybe desired to inhibit fertility, for instance.

[0351]Typically, promoter or control elements which provide preferential transcription in flowers produce transcript levels that are statistically significantly altered as compared to other cells, organs or tissues.

[0352]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0353]Immature Bud and Inflorescence Preferential Transcription

[0354]Promoters and control elements providing preferential transcription in an immature bud or inflorescence can time growth, development or maturity or modulate fertility or viability in host cells or organisms. In a plant, preferential modulation of genes, transcripts, and/or polypeptide in an immature bud or inflorescence is useful, [0355](1) to modulate embryo development, size, and maturity; [0356](2) to modulate endosperm development, size, and composition; [0357](3) to modulate the number of seeds and fruits; or [0358](4) to modulate seed development and viability.

[0359]Up-regulation and down-regulation of transcription is useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to decrease endosperm size, for instance.

[0360]Typically, promoter or control elements which provide preferential transcription in immature buds and inflorescences produce transcript levels that are statistically significantly altered as compared to other cell types, organs or tissues.

[0361]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0362]Senescence Preferential Transcription

[0363]Promoters and control elements providing preferential transcription during senescence can be used to modulate cell degeneration, nutrient mobilization and scavenging of free radicals in host cells or organisms. Other types of responses that can be modulated include, for example, senescence associated genes (SAG) that encode enzymes thought to be involved in cell degeneration and nutrient mobilization (Arabidopsis; Hensel et al. (1993) Plant Cell 5: 553-64), and the CP-2/cathepsin L gene (rat; Kim and Wright (1997) Biol Reprod 57: 1467-77). Both of these genes are induced during senescence.

[0364]In a plant, preferential modulation of genes, transcripts and/or polypeptides during senescencing is useful to modulate fruit ripening.

[0365]Up-regulation and down-regulation of transcription are useful for these applications. For instance, genes, transcripts and/or polypeptides that increase scavenging of free radicals may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit cell degeneration, for instance.

[0366]Typically, promoter or control elements which provide preferential transcription in cells, tissues or organs during senescence produce transcript levels that are statistically significantly altered as compared to other conditions.

[0367]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0368]Germination Preferential Transcription

[0369]Promoters and control elements providing preferential transcription in a germinating seed can time growth, development or maturity or modulate viability in host cells or organisms. In a plant, preferential modulation of genes, transcripts and/or polypeptide in a germinating seed is useful [0370](1) to modulate the emergence of they hypocotyls, cotyledons and radical; or [0371](2) to modulate shoot and primary root growth and development;

[0372]Up-regulation and down-regulation of transcription is useful for these applications. For instance, genes, transcripts and/or polypeptides that increase growth may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to decrease endosperm size, for instance.

[0373]Typically, promoter or control elements which provide preferential transcription in a germinating seed produce transcript levels that are statistically significantly altered as compared to other cell types, organs or tissues.

[0374]For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.

[0375]Results

GFP Experimental Procedures and Results

Procedures

[0376]The polynucleotide sequences of the present invention were tested for promoter activity using Green Fluorescent Protein (GFP) assays in the following manner.

[0377]Approximately 1-2 kb of genomic sequence occurring immediately upstream of the ATG translational start site of the gene of interest was isolated using appropriate primers tailed with BstXI restriction sites. Standard PCR reactions using these primers and genomic DNA were conducted. The resulting product was isolated, cleaved with BstXI and cloned into the BstXI site of an appropriate vector, such as pNewBin4-HAP1-GFP (see FIG. 1).

[0378]Transformation

[0379]The following procedure was used for transformation of plants

1. Stratification of WS-2 Seed.

[0380]Add 0.5 ml WS-2 (CS2360) seed to 50 ml of 0.2% Phytagar in a 50 ml Corning tube and vortex until seeds and Phytagar form a homogenous mixture. [0381]Cover tube with foil and stratify at 4° C. for 3 days.

2. Preparation of Seed Mixture.

[0381] [0382]Obtain stratified seed from cooler. [0383]Add seed mixture to a 1000 ml beaker. [0384]Add an additional 950 ml of 0.2% Phytagar and mix to homogenize.

3. Preparation of Soil Mixture.

[0384] [0385]Mix 24 L SunshineMix #5 soil with 16 L Therm-O-Rock vermiculite in cement mixer to make a 60:40 soil mixture. [0386]Amend soil mixture by adding 2 Tbsp Marathon and 3 Tbsp Osmocote and mix contents thoroughly. [0387]Add 1 Tbsp Peters fertilizer to 3 gallons of water and add to soil mixture and mix thoroughly. [0388]Fill 4-inch pots with soil mixture and round the surface to create a slight dome. [0389]Cover pots with 8-inch squares of nylon netting and fasten using rubber bands. [0390]Place 14 4-inch pots into each no-hole utility flat.

4. Planting.

[0390] [0391]Using a 60 ml syringe, aspirate 35 ml of the seed mixture. [0392]Exude 25 drops of the seed mixture onto each pot. [0393]Repeat until all pots have been seeded. [0394]Place flats on greenhouse bench, cover flat with clear propagation domes, place 55% shade cloth on top of flats and subirrigate by adding 1 inch of water to bottom of each flat.

5. Plant Maintenance.

[0394] [0395]3 to 4 days after planting, remove clear lids and shade cloth. [0396]Subirrigate flats with water as needed. [0397]After 7-10 days, thin pots to 20 plants per pot using forceps. [0398]After 2 weeks, subirrigate all plants with Peters fertilizer at a rate of 1 Tsp per gallon water. [0399]When bolts are about 5-10 cm long, clip them between the first node and the base of stem to induce secondary bolts. [0400]6 to 7 days after clipping, perform dipping infiltration.

6. Preparation of Agrobacterium.

[0400] [0401]Add 150 ml fresh YEB to 250 ml centrifuge bottles and cap each with a foam plug (Identi-Plug). [0402]Autoclave for 40 min at 121° C. [0403]After cooling to room temperature, uncap and add 0.1 ml each of carbenicillin, spectinomycin and rifampicin stock solutions to each culture vessel. [0404]Obtain Agrobacterium starter block (96-well block with Agrobacterium cultures grown to an OD₆₀₀ of approximately 1.0) and inoculate one culture vessel per construct by transferring 1 ml from appropriate well in the starter block. [0405]Cap culture vessels and place on Lab-Line incubator shaker set at 27° C. and 250 RPM. [0406]Remove after Agrobacterium cultures reach an OD₆₀₀ of approximately 1.0 (about 24 hours), cap culture vessels with plastic caps, place in Sorvall SLA 1500 rotor and centrifuge at 8000 RPM for 8 min at 4° C. [0407]Pour out supernatant and put bottles on ice until ready to use. [0408]Add 200 ml Infiltration Media (IM) to each bottle, resuspend Agrobacterium pellets and store on ice.

7. Dipping Infiltration.

[0408] [0409]Pour resuspended Agrobacterium into 16 oz polypropylene containers. [0410]Invert 4-inch pots and submerge the aerial portion of the plants into the Agrobacterium suspension and let stand for 5 min. [0411]Pour out Agrobacterium suspension into waste bucket while keeping polypropylene container in place and return the plants to the upright position. [0412]Place 10 covered pots per flat. [0413]Fill each flat with 1-inch of water and cover with shade cloth. [0414]Keep covered for 24 hr and then remove shade cloth and polypropylene containers. [0415]Resume normal plant maintenance. [0416]When plants have finished flowering cover each pot with a ciber plant sleeve. [0417]After plants are completely dry, collect seed and place into 2.0 ml micro tubes and store in 100-place cryogenic boxes.

Recipes:

[0417] [0418]0.2% Phytagar [0419]2 g Phytagar [0420]1 L nanopure water [0421]Shake until Phytagar suspended [0422]Autoclave 20 min

YEB (for 1 L)

[0422] [0423]5 g extract of meat [0424]5 g Bacto peptone [0425]1 g yeast extract [0426]5 g sucrose [0427]0.24 g magnesium sulfate [0428]While stirring, add ingredients, in order, to 900 ml nanopure water [0429]When dissolved, adjust pH to 7.2 [0430]Fill to 1 L with nanopure water [0431]Autoclave 35 min

Infiltration Medium (IM) (for 1 L)

[0431] [0432]2.2 g MS salts [0433]50 g sucrose [0434]5 ul BAP solution (stock is 2 mg/ml) [0435]While stirring, add ingredients in order listed to 900 ml nanopure water [0436]When dissolved, adjust pH to 5.8. [0437]Volume up to 1 L with nanopure water. [0438]Add 0.02% Silwet L-77 just prior to resuspending Agrobacterium

[0439]High Throughput Screening--T1 Generation

1. Soil Preparation. Wear gloves at all times. [0440]In a large container, mix 60% autoclaved SunshineMix #5 with 40% vermiculite. [0441]Add 2.5 Tbsp of Osmocote, and 2.5 Tbsp of 1% granular Marathon per 25 L of soil. [0442]Mix thoroughly.

2. Fill Com-Packs With Soil.

[0442] [0443]Loosely fill D601 Com-Packs level to the rim with the prepared soil. [0444]Place filled pot into utility flat with holes, within a no-hole utility flat. [0445]Repeat as necessary for planting. One flat set should contain 6 pots.

3. Saturate Soil.

[0445] [0446]Evenly water all pots until the soil is saturated and water is collecting in the bottom of the flats. [0447]After the soil is completely saturated, dump out the excess water.

4. Plant the Seed.

5. Stratify the Seeds.

[0447] [0448]After sowing the seed for all the flats, place them into a dark 4° C. cooler. [0449]Keep the flats in the cooler for 2 nights for WS seed. Other ecotypes may take longer. This cold treatment will help promote uniform germination of the seed.6. Remove Flats From Cooler and Cover With Shade Cloth. (Shade cloth is only needed in the greenhouse) [0450]After the appropriate time, remove the flats from the cooler and place onto growth racks or benches. [0451]Cover the entire set of flats with 55% shade cloth. The cloth is necessary to cut down the light intensity during the delicate germination period. [0452]The cloth and domes should remain on the flats until the cotyledons have fully expanded. This usually takes about 4-5 days under standard greenhouse conditions.

7. Remove 55% Shade Cloth and Propagation Domes.

[0452] [0453]After the cotyledons have fully expanded, remove both the 55% shade cloth and propagation domes.8. Spray Plants With Finale Mixture. Wear gloves and protective clothing at all times. [0454]Prepare working Finale mixture by mixing 3 ml concentrated Finale in 48 oz of water in the Poly-TEK sprayer. [0455]Completely and evenly spray plants with a fine mist of the Finale mixture. [0456]Repeat Finale spraying every 3-4 days until only transformants remain. (Approximately 3 applications are necessary.) [0457]When satisfied that only transformants remain, discontinue Finale spraying.

9. Weed Out Excess Transformants.

[0457] [0458]Weed out excess transformants such that a maximum number of five plants per pot exist evenly spaced throughout the pot.

[0459]GFP Assay

[0460]Tissues are dissected by eye or under magnification using INOX 5 grade forceps and placed on a slide with water and coversliped. An attempt is made to record images of observed expression patterns at earliest and latest stages of development of tissues listed below. Specific tissues will be preceded with High (H), Medium (M), Low (L) designations.

TABLE-US-00002 Flower Pedice, I receptacle, nectary, sepal, petal, filament, anther, pollen, carpel, style, papillae, vascular, epidermis, stomata, trichome Silique Stigma, style, carpel, septum, placentae, transmitting tissue, vascular, epidermis, stomata, abscission zone, ovule Ovule Pre-fertilization: inner integument, outer integument, embryo sac, funiculus, chalaza, micropyle, gametophyte Post-fertilization: zygote, inner integument, outer integument, seed coat, primordial, chalaza, micropyle, early endosperm, mature endosperm, embryo Embryo Suspensor, preglobular, globular, heart, torpedo, late, mature, provascular, hypophysis, radicle, cotyledons, hypocotyl Stem epidermis, cortex, vascular, xylem, phloem, pith, stomata, trichome Leaf Petiole, mesophyll, vascular, epidermis, trichome, primordial, stomata, stipule, margin

[0461]T1 Mature: These are the T1 plants resulting from independent transformation events. These are screened between stage 6.50-6.90 (means the plant is flowering and that 50-90% of the flowers that the plant will make have developed) which is 4-6 weeks of age. At this stage the mature plant possesses flowers, siliques at all stages of development, and fully expanded leaves. We do not generally differentiate between 6.50 and 6.90 in the report but rather just indicate 6.50. The plants are initially imaged under UV with a Leica Confocal microscope. This allows examination of the plants on a global level. If expression is present, they are imaged using scanning laser confocal micsrocopy.

[0462]T2 Seedling: Progeny are collected from the T1 plants giving the same expression pattern and the progeny (T2) are sterilized and plated on agar-solidified medium containing M&S salts. In the event that there was no expression in the T1 plants, T2 seeds are planted from all lines. The seedlings are grown in Percival incubators under continuous light at 22° C. for 10-12 days. Cotyledons, roots, hypocotyls, petioles, leaves, and the shoot meristem region of individual seedlings were screened until two seedlings were observed to have the same pattern. Generally found the same expression pattern was found in the first two seedlings. However, up to 6 seedlings were screened before "no expression pattern" was recorded. All constructs are screened as T2 seedlings even if they did not have an expression pattern in the T1 generation.

[0463]T2 Mature: The T2 mature plants were screened in a similar manner to the T1 plants. The T2 seeds were planted in the greenhouse, exposed to selection and at least one plant screened to confirm the T1 expression pattern. In instances where there were any subtle changes in expression, multiple plants were examined and the changes noted in the tables.

[0464]T3 Seedling: This was done similar to the T2 seedlings except that only the plants for which we are trying to confirm the pattern are planted.

Image Data:

[0465]Images are collected by scanning laser confocal microscopy. Scanned images are taken as 2-D optical sections or 3-D images generated by stacking the 2-D optical sections collected in series. All scanned images are saved as TIFF files by imaging software, edited in Adobe Photoshop, and labeled in Powerpoint specifying organ and specific expressing tissues.

Instrumentation:

Microscope

Inverted Leica DM IRB

[0466]Fluorescence filter blocks: [0467]Blue excitation BP 450-490; long pass emission LP 515. [0468]Green excitation BP 515-560; long pass emission LP 590

Objectives

[0468] [0469]HC PL FLUOTAR 5×/0.5 [0470]HCPL APO 10×/0.4 IMM water/glycerol/oil [0471]HCPL APO 20×/0.7 IMM water/glycerol/oil [0472]HCXL APO 63×/1.2 IMM water/glycerol/oil

Leica TCS SP2 Confocal Scanner

[0472] [0473]Spectral range of detector optics 400-850 nm. [0474]Variable computer controlled pinhole diameter. [0475]Optical zoom 1-32×. [0476]Four simultaneous detectors: [0477]Three channels for collection of fluorescence or reflected light. [0478]One channel for transmitted light detector. [0479]Laser sources: [0480]Blue Ar 458/5 mW, 476 nm/5 mW, 488 nm/20 mW, 514 nm/20 mW. [0481]Green HeNe 543 nm/1.2 mW [0482]Red HeNe 633 nm/10 mW

Results

[0483]The section in Table 1 entitled "The spatial expression of the promoter-marker-vector" presents the results of the GFP assays as reported by their corresponding cDNA ID number, construct number and line number. Unlike the microarray results, which measure the difference in expression of the endogenous cDNA under various conditions, the GFP data gives the location of expression that is visible under the imaging parameters. Table 3 summarizes the results of the spatial expression results for each promoter.

Explanation of Table 1

[0484]Table 1 includes various information about each promoter or promoter control element of the invention including the nucleotide sequence, the spatial expression pattern associated with each promoter and the corresponding results from different expression experiments.

TABLE-US-00003 Lengthy table referenced here US20110041208A1-20110217-T00001 Please refer to the end of the specification for access instructions.

TABLE-US-00004 Lengthy table referenced here US20110041208A1-20110217-T00002 Please refer to the end of the specification for access instructions.

[0485]The invention being thus described, it will be apparent to one of ordinary skill in the art that various modifications of the materials and methods for practicing the invention can be made. Such modifications are to be considered within the scope of the invention as defined by the following claims.

[0486]Each of the references from the patent and periodical literature cited herein is hereby expressly incorporated in its entirety by such citation.

TABLE-US-LTS-00001 LENGTHY TABLES The patent application contains a lengthy table section. A copy of the table is available in electronic form from the USPTO web site (http://seqdata.uspto.gov/?pageRequest=docDetail&DocID=US20110041208A1). An electronic copy of the table will also be available from the USPTO upon request and payment of the fee set forth in 37 CFR 1.19(b)(3).

Sequence CWU 1

11712039DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0001 as found in Promoter Report #1 1ctgcattcac acatattttg ggctctcacg tgtttgtgaa tttaatatat ttgactacac 60gatctttcaa cgtatgaaaa agttttatac tactattttc gtttgagtgg gaaataaaca 120aatgatagct acagttatct atatggtata attttacact tttataacta ataatgatga 180gtgatgacaa tcgagtgtcg gatataacag gccaacaagt ggaatggact tatgtaactt 240tttaatcacg ggattaaatc acgtaaccca atgtcctaat tggtatttaa ttttgattat 300ctcgatgcta catattgtca taggactcat atctttgatc acgtgccgct accaatccag 360acattttagt atacaaaaaa aaagaagata caaacttaag atatggaata tatatcagaa 420ctatcagttt tagactttaa taattcgaat tgaataacta cgatcaatat ataaattggc 480aaatagattg gtcaattgta gtgcaagaaa tttgtgaact ttattacagt acgaagagag 540taagagaagc aagatccggt ttttaggcaa caagtaacat ttttgagttc agagagtttg 600cttcttactt taagttacgt cactacaaaa gccaagttcc tacttcttag gtctaaagtc 660aattttcgaa tattcagaaa aattgtactc tactagatcg aatagttttc accggtgaaa 720cgatatataa atgaagacta caatattttt taattttttt aagcgtatga gttctagacc 780tttggcacgt aaatttctcc ggtacctggg accaatcgtt gataatatca cgtttaagat 840ttaatcatcc atcccaagta gagttgaact agtaaccttg agcacttttt ctcgagacaa 900ctaaaccatc atccacttag tgcaataaag cgtcattctt ttttttcttt tcaaaaattc 960gtatttaatt ttaatttatt aaaaatattt cttttgtttt aaattgggac agaattatca 1020tttaacatat ttaaaattta tatttttaat taaaaatagg gtaaaatata tttttcaaac 1080aaaaattcaa aaatagggca attttcaaaa tcatccattc ttaaatctaa agtcggctac 1140agtcttttcg ttgttttgtt gctaatttca atttatatac atgcaaatta caaaatataa 1200tagtttttgg gggataatta tcttcttgcg cctttttatt aaattaatat gctcatatag 1260cagttcttac aattaatata actagggttt taaatttcaa tatcgagttg acaaaatgaa 1320ttgtttacaa gtttttttct tttcaatatg cattgttcat cacgtattcg tagtgatgca 1380aaaacaaact ataaattata attgcactag tgagattagc aagaagtgtt ataaattaga 1440ataaacggaa ctatcaaact gtgttatgta caccatttat ttttgttaaa gaatatgtgt 1500agtagttaga aaactgatca aattaaactg aaaattcaca ttacggagat caagttacat 1560tgtctattga tgaaaaaaac aaaataaatc caaatggcac taaaagttgt agaaattgaa 1620agaagaaaat agatttttgt ctaggaataa aagtcaaaat gggaaagaca aaaaaaagag 1680aggcaaataa gcagtgatgg agctaaagca acgctttact cttttaatta tgaattattt 1740gatttgacct ccactcgcct ggcttttttt ggttgttctt tatagaaaag taaaataaca 1800caattagcac ataacatgag ttatcgagaa accaattctc tttgtggtgt tttagttaat 1860ttctataact tatgaaacca ttttctcagt ttatcatgat aattgatcct ctatttaaaa 1920ccctaaagtt tatattttgt ttgttcaaac acagtcgcca ttgcactggg atccaacaat 1980gtcctccgac tcgtccaaga tcaagaggaa gcggaaccgc atncctttaa cgaaggcga 203921129DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0007 as found in Promoter Report #2 2agcagaacaa ctatatttat tgtgtcacat aaatctgaga tcatttataa ccaccaaaga 60acctatacac agtaaatgac aaatgtatct ccctctatct ctattgccca tatgtagatg 120ctaaagtaag atttctcttt tttttaatgt actttttttt gtataaagta tattccataa 180gaaaaaggaa aagcttgttt atggatcaat tgaccccaaa aaaagttttt agatcaaagc 240ccaatataaa aaaaaaacac agtagtgaca caaaggaact taaataaacc atgaattgat 300ctataaacag tagagatcga taaggcgaac attttccatg tgaagtgtct tctttcatct 360ataatatttt tgacatccaa taatttcctc tataatatca ttcacataat tgatagaaac 420attatgttag aattgtccac atcatttgag ctgtaatata ttctgtttta acaaattata 480tggtagttgc ttaatcttat gtccatcttc ttctatgcat cgttttcgcg cctagttgtc 540cagtccattt caactaccta cctctaattc ttatcttaaa acaacatttt ttaatttaag 600tattatgctc aaagactaac tagatagaaa accgttatta aacattaaac gaattaaaag 660tcttacatgg aaaatgtagg tttataaacc acgagttatg attgacaata aaaaaaatgc 720aaatcatcaa tcaaaagaga cttgagtgcg actctatatc aaccattgca attaaaatta 780tctatcacaa aaattttaga cagattaagt taatttagtc taaattcact aatttatttt 840ctataattag taattaacta tatttattta tttacacatt ttctgataat ttagaaattt 900gcatgaataa caaatataag attttggaaa ttagtagcaa atttaattaa taattatttt 960tgcctaaatg aaccaaacta taaaacctcc acatacacca gtcatcaaat ttacagagac 1020aacaaactaa agttggtggt gatagagtga gagagaaaca ccattgcact gggatccaac 1080aatgtcctcc gactcgtcca agatcaagag gaagcggaac cgcatcccg 112931035DNAArabidopsis thalianamisc_featureCeres Promoter construct G0013 as found in Promoter Report #3 3atcttgtgat acacaattta ttactatttg gtacattttg aagtatttgt ttttgcatga 60tatatgacgt taatttgaac tgatattagt caatttatgg gtacaaaagt tgaaagttta 120gagcactatg ttggatttat taaaaatgat atcatacaat ggttcaatat atatatattt 180ttttccacgt ttttaataac atttttgtaa acaagtcttc tactattgtc tttattgtta 240atgagtttct agtacctaat taggaatttt gaggatatac gatacattaa tgagttacat 300tatcccgaaa acaaaatctt gaaaacgaac aaagataatt tggacattac tcgttatgta 360tacgtatgga attggataga gccgttgaac catcaagtgg gtcttcaagt caacgaactg 420aatttgattt tacactcatg tacatcggcc acaattttat tcacacacta ctaacacctc 480tggtgtccac ttttttcttt ctctagattg atgtgttaag atttttgttg caattcattt 540attcaggtat ttttatatat atatatatat aaattagaat aaactaattt aaagaaagat 600atagcaatta tgtttcacat tttaacattc tcaatcattt ataaaactaa tgtggtgatg 660aatggtatat atatatatat atatatatat atatacatat atatattttg ttgtgaacta 720atggtaaata tttaaaataa gacatacgta cataaatcca cgggctctta aagtcatgat 780gcggttaata aatgttcaca taacggtaac caagtggctc aaaatcatga aacaacgtca 840cataatttat cttataatgt ggataattag taccgcatta tttgtaagaa aattaaatta 900attatagatt cacagctaag aaaatacgaa aagacagctc aacacttttc cacttctatt 960ccccactgtc tatataactc tgataaataa tctctgatct ctccaccatt gcactggtcc 1020aggagataaa caaga 103541000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0097 as found in Promoter Report #4 4ttcatcttta tatttaagag tttaaaaact gcaacttttg tttttctttc actaagtctt 60atggccacag ttaattaaaa gcagatgaaa ggtggtccaa tggaaaagga gaatgtgatt 120gggctagttg ggagagttct gatgtctagt gttgggtaca cgtgtccgtc agttacacat 180agcattaaat cagacggcat gtcattattc aaatctagtt cacatagtac gactaatagc 240tgataaatta atgattatac agcatatgaa ttatgaattc aaaaaaaaaa aaaaattgaa 300aatgttaagg agatgctata ttttacaaaa ttcatcgcaa tgctttctac taatttgcta 360agtggtcttc tccagttagt cttgtcgatt ccaagcgata ttattaaatc ttgaagcatc 420gctcaaagca ttatagctta agataaccaa attgttatta aaaacaccta gtgaaatttt 480taaattaaaa caattttgat atctttgtaa tatctaatac tactctttct gtgtctaaaa 540ggattaattt tcaaaaattt cacacatatt aaaaaaaaaa aaaaattact agctaaacaa 600ttttcaataa tcataaaaca atagtaactt aataattttt ttttattttc aaaatagtcc 660ttcaagttta caattcattt tagtattata atcaacaaaa tttgtattaa aaagttggaa 720aattaatctt tgtggaacaa aaaaatctag aaatcatttt ttagaattag agagaggttt 780gataaaaaaa aataaaaaaa aatagagaga ggtagtacat actaaacgat gtgatactac 840tattgacaaa atcttaattc tcagtttagt agaataaact agaaggaatg aatgaagtaa 900atgcgaatcc aactactaac aaaccctact tagtcatcat attttcccat atgaaatccc 960tatataaacc catcatcatc tcccactttt ttcatatcca 100051068DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0111 as found in Promoter Report #5 5cgattggatt tagtctatac attatagggc gcaagtttgt ggatttaaga attatataaa 60aacttgaaat atatagtttt tatgcattct cctcttgtgt aatacataaa ccaaatatga 120gataggttaa tctgtatttc agataatatt aaattccaaa caatattttt acttgttata 180agaaggcaat taatatctct ctgttaatgg caagtggtac caagtagtat taaactatta 240atgcaatgga agagtactgt tggaaattat aatcctctat cacacattca aacagatctc 300ctgaaatctt ctcttccaaa cttgtacttc tctgatccaa atgtaggctc caaaatatag 360acatttacca tttactaagt ccacaactcc tttcttgtct ccttcaaaaa tgactcttgt 420gtaaccacca tatgactccg acagttcggc attgccatga tgagagctta aaaattcacc 480ttcctgagca tttcaagtct tcactccctt agcttgacct gaaccaagat aaaatgcctt 540tgtcgtcccg taatatccat cctgctttgg acggcatcat agttacattc gatccatcct 600atttacaatg ttattttagt attaaaaaca tgacaataaa tttgttgtta aacatattca 660aatacaatat gattggattt ataagtaatt gtaatatgaa atgtccttag taatatgtta 720aaaaatacat agatacacac acgtactaaa agaggcaacg cgggagatgt cattagagga 780agaactagga agcagagcgt tcatgcaaaa tgctaccaaa aacgttaatg caatatctca 840actaatcagc acagtccatt tcatactgag aatgtaaaaa ccaatcagca tcgtccattt 900tttcatctaa ttatttgtta actcttaatt ggccacaact tccaaccaca tgacgctctt 960tctattccct ttatatattc ccatctcaaa tgttcttgga gacacaaaat atcataaaca 1020tataaacata aacgccaatc gcagcttttg tacttttggc ggtttaca 106861002DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0104 as found in Promoter Report #6 6tttattttat tttttgaatg aaaatgtctt ctttattcgt aattttaaac tcactggtgg 60tggatatatt gttatgtccc caattcgtct ggcaactctc gtatattagt gagaaaaatt 120tgtccattat ttactgcact attaccctgt gttaattttt tgtattgaaa ttgttttttt 180agtaattcac gtcatatagc gaatgattct ttaattttaa aaattcagtc ttaagtttac 240aaattaaata acgctactgt aaccaactct gtacgaccaa catgttcgag tttttgtata 300tacggccata tatgtacata ttttactata aagcgaaaaa atccataaat tatttaatta 360atatataaag gtgccattct atttccaatg tgcttaggaa aatgcagaac ctcgtgctat 420atctctgtcg ccacgtgcaa atataacaat atgaaataga actagcaaat cttgaaatct 480aactcttaag actaattcaa gcacatacgt agagaaagtt gaccaacggt tatcagcatt 540ttaacatgga ccttatcaac attttaacaa agtccacaaa caaccagtct tacaatcgca 600ttggtacaag ataatcgaat tcatcttcca tataacaaaa cctaaacctt ggtgtgaaaa 660ggagaagata tgtatgttaa aggccgccta tgcctctggt ttggggtata tgattctaag 720attagggttt gaatattttc gttagcctgc catgagatat atttatgtga taatttagag 780cctcttatgc attaatgcat aaccgactag atccatgtgg tattcagcta atcagtacac 840acaagacaaa gtagtaaatg agtttgatga agactgtggt ctgataattc ctatcaacgt 900taaatctgtc ggggccaggc agccagcaac attttgccta ccaacgctct gaattcaatt 960gaacctaggc tatataatag caggctaact taactaagag tt 100271000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0075 as found in Promoter Report #7 7tggattacaa atcattaagc taatatcttc gatgaattaa gaagataagt ggataacaag 60tacctaaccg caatagtcca taaattaaaa cattaatgta tttgtcgttg aaaatttggc 120cgacttttat ttgttattct agtttccaca tcaaaaatgt ttgtacttcg tagcaatcca 180tccacctaaa ccccaaatct taatttatat ttgttgcgtt taaatttggg tgagatttga 240ttctaagtag ttgagataaa ttgatattct attcattagt aaaatgatag agaaattggt 300ttataataat tttaccctag aacatgacat gatattggta accattaatc aaagaaagag 360caaagcattt aatttaccct actctccaac cactccagcc tttattagtt gcagttggga 420atcatttctt tatgattctt atgtcattgt ctcctaaatc aatgaagtgc cttgaccttg 480ttactaattc gaacatagca aagccaacta catagatcct ttacaaagtt ctaaaaacag 540gttgtttagg cgtctagaca aacaaaacca ttttgtacga ttcaacaaat tggtccatag 600aatgttattg atctttcttg tttaggcatt cgataaatcg gctaatacat tatttttttg 660ttttgctttt tccttattaa aaatatgcaa agtattatga tgtttaacct gaactgaatt 720ttacatttaa ctggatatag gaaaatattg ggttgaattt aataattaag caattgtcac 780gtaaatcaaa ttgggcttaa tatatattgt tgatttcagc aaagacaaag ttgggccgtt 840tcaatagtct tcacgcgatg taagcgttca ctaaccaact agagaagaca atcaaatgaa 900tacgttccac gtgacgctta cgaacttgtc agtcactttg gtaatatgac agacagtaac 960cagtaaacta ctaatctctt tcgctaacga acacacaaaa 10008998DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0016 as found in Promoter Report #8 8aaacatgttt tatgtaacta ctttgcttat gtgattgcct gaggatacta ttattctctg 60tctttattct cttcacacca catttaaata gtttaagagc atataaatta attatcttca 120aaaaggtgat tatatgcatg caaaatagca caccatttat gtttatattt tcgaattatt 180taatacattt caatatttca taagtgtgat tttttttttt tttgtcaatt tcataagtgt 240gatttgtcat ttgtattaaa caattgtatc gcgcagtaca aataaacagt gggagaggtg 300aaaatgcagt tataaaactg tccaataatt tactaacaca tttaaatatc taaaaagagt 360gtttcaaaaa aaattctttt gaaataagaa aagtgataga tatttttacg ctttcgtctg 420aaaataaaac aataatagtt tattagaaaa atgttatcac cgaaaattat tctagtgcca 480ctcgctcgga tcgaaattcg aaagttatat tctttctctt tacctaatat aaaaatcaca 540agaaaaatca atccgaatat atctatcaac atagtatatg cccttacata ttgtttctga 600cttttctcta tccgaatttc tcgcttcatg gttttttttt aacatattct catttaattt 660tcattactat tatataacta aaagatggaa ataaaataaa gtgtctttga gaatcgaacg 720tccatatcag taagatagtt tgtgtgaagg taaaatctaa aagatttaag ttccaaaaac 780agaaaataat atattacgct aaaaaagaag aaaataatta aatacaaaac agaaaaaaat 840aatatacgac agacacgtgt cacgaagata ccctacgcta tagacacagc tctgttttct 900cttttctatg cctcaaggct ctcttaactt cactgtctcc tcttcggata atcctatcct 960tctcttccta taaatacctc tccactcttc ctcttcct 99891000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0094 as found in Promoter Report #9 9taaagatcag aagaggaagg tttcgccgcg gcggttgcat cttcaccgtc gatttcatcg 60ttacagcgac gccggtaatt cctaggttgc ttagttccca ttctctctct aaaattaggg 120ctcgaaatga attgttgaac aagatagaga tctttttctg atccccgtcg aacatttatt 180caaggccaaa aaaagcacac gggaatttag agtaccaata catatcaaaa cctaatgggc 240tttgaatggt tgcatgtgtg tgtttatttc tgatatgcaa agcgatcgat agtcttttcc 300atacaagtgt aaactgtaaa caacgtaatt aagcataaca atacaactct ttcttctctt 360tttttttgta aacacaaaat aaaattacat caattcatgc ttttcctagt tcatctgaca 420ttttccaaaa ttcatgttcc attgagtccc taatacttgt tcatattcat attagggtac 480atgaataaaa gttatcattc ttgaaactac taaattttca tagtttattt ttcttctttt 540cgtttcactt tcgaacaaaa cactacgcgt ggcatttgca atgaattcca cattatatgg 600aataacacca tgatgaacat tctacatata taattattat gtttaagcac ttagacagca 660taaattcttt ctaattatat aaatctaacc ttgttacatt gtacatctat aaattacttg 720aagaaataac gagttctatt tctttttaaa aattaaaaat actataccat atctcagtga 780ttaagttgaa ccaaaaggta cggaggagaa acaagcattt gattcttcct tattttattt 840tattcatctc tcactaatga tggtggagaa aaaaagaaaa tacctaacaa acaaatatat 900attgtcatac aaaaatattt ctatattttt agttaattag tttatattcc tcacttttca 960gggcttatat aagaaagtga gcaaacacaa atcaaaatgc 100010691DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0033 as found in Promoter Report #10 10gtcgattgga acttccaatt tctaaacgga tgcaataaga acttacatat tctctttcat 60tagtcattta ttggccagat ttattaaaaa aagttttact caatgaccaa ggattagagt 120taaagataat atagattatt acatatatta ttcgaaaaaa tatacccatg tccgactttt 180taaacctcaa aaatatcaaa accagaaaag atgatacaac acaaaaaaac aataaaataa 240taagtggaag agatatcatc ggacaacagt acaagtacag caccagctct gccaaaagcc 300aaaaccattt gtcaattaca gaaagatact aatgtttgaa attactaaat tacccctcgg 360actttacaaa agcatctcta acttatccac gtgtcagtca tctattgatt gtttcaatac 420caccttgtat taacgcccca cgattcgtgg ttgggtacac ctgatagtcc gaggatattt 480aaatctcacg cgctcgtgtc tataattcga ctgtactcgc ttttcttgtc gtgattttag 540caatttacga agtcaaatgt ttgactcaat cagacttgcg cataggagag cgagtataaa 600tgtttactat actcacgcaa gtggggcttt attgaaacta ctcttttgta ataaaaccag 660cagtggtttt gttctgaatc cgctctcttg c 69111992DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0049 as found in Promoter Report #11 11ttccaaattc ttatggttct ctagtgtcat gattttgaga atcactcaac tccaaaaata 60taatccacga tcccgtgtta attattgaag aatcaatcgt ttttaatttc tcaccaatag 120atgttgctct tattacttaa aacaaattgt tcggacaaat gtagcaagtg tgatactttg 180tgggatctta aagacgattt ctcctataac agaggacaaa acaggtcggt caattacaat 240gtcatccctc tttgccctgt cttttttttt cttcttaaaa cctaaccatt tgattgtttc 300taaaggtatt tcaagaatat atgatcaatc tagatgaata ctataccgac gatgactaca 360cacacaagga aatatatata tcagctttct tttcacctaa aagtggtccc ggtttagaat 420ctaattcctt tatctctcat tttcttctgc ttcacattcc cgctagtcaa atgttaataa 480gtgcacacaa cgttttctcg aagcattaga atgtcctcct cttaattaat ctccttctga 540ttagattctc aatagagttt aaatttgtta atggagagat atattgggac cctcaaggct 600tctaattata ccacgtttgg cataattctc tatcgtttgg ggccacatct ttcacacttc 660attaccttat caccaaaaca taaaatcaat caactttttt ttgccttatt gattgtgttg 720gatccctcca aaattaaaac ttgtgttccc cacaaaagct tacccaattt cacttcaatc 780ttaacaaata ggaccaccac taccacgtac ggtttgcatc atacaaacca caaactcctt 840cttcattaca attattatat catctactaa aacctctttc tccctctctc tttcttgttc 900ttagtgctaa attttctttg ttcaggagaa atatccattg cactgggatc caacaatgtc 960atccgactcg tccaagatca agaggaaagc gg 992121000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0060 as found in Promoter Report #12 12tggagcttta ttgaaatgca agaaagtaaa caaaggaaga tctttagatt gtcaccaaga 60gtggtctgaa actctcataa cactcaatcc tcctcctcct catcaccacc actacaaaat 120attatattct ctatctctca atctatgagg agatgtattc tatcaagcat ttgaaatgat 180aagaaactgg cgatcatcct ctacgtcacc atcactccaa aattatcctc tttctaggtt 240taagttttgt aatgatcgcc tttatttgtt gagatctcta acttctcgca tttccaaaat 300gttaagtcca ataactgcat tggttaagtt ggggcgttac tagtcggctt aaatccaaat 360atggatttga ttccatatgt atgtgacagt ttcttaacgt tcatattaca atgaatgatg 420gatccttgac tagacaaaga gaaaatggat tgtcacttcg taggaaaaat agaaattctc 480cacgaaggct ggtctccttt atttaacgac aaattcactc atagtctcat tcacaatttg 540aacttgtcta acacaatgtg ttatatactc gcgaaaagaa gcataatagg ctcttaaggg 600taatccacga aaccaaaaca catataaaac attaatattt ttctctaaat ttattcatat 660caataataaa gtttacaaaa aatataaaac aataatccat acttagccca tagcttcgtg 720tggaagaaga cttgattttt gactagtcaa cgaaaatgag taaatgacgt attcagctat 780agtaaaaggg atcataagcg gaaattacaa agaagctttg agggtaaact agtcaaaaag 840cataatcaga aataacttag gcccaaagca aaaaggaaag ggctctggat ccagccgcaa 900atcagaatct ggtaagttcg aacgccacgt catcacctaa atatctgaaa tatctaatta 960agacttgtct atatataaag gcttctcctt tcacaatccc 1000131069DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0092 as found in Promoter Report #13 13aaagattgag ttgagagaga tggtggagac gcagaacaga caaagggagt ttaccatata 60gtgctctaaa gggcaatgag attgcagtga tgtggctatc cggggaatca tcgcaggtta 120ttccttccca tgagcaacaa tcaatggatg ggttccaatt cagaggagaa acagaagaag 180aaacgtttcc agagaaccac agtagggatt ctcgatcttg cgagttgcag agagcctctg 240aaactgcaat agaaaggaca ctgatgaaaa gaacacactg aaggagtatg ccaatcatgt 300gaaaactcag agcttgtatt ggtcttgtgg ttgatgaagt tctcacaaaa cctttggctt 360tgaatctccc ctcattagtc atggtgagaa caagaacaag acgagaaaca gacaaagaag 420atgaaaaaac ttgttggcca gtgttgacta agggggaata gccccagaca taacaaaatt 480agacttgtcg tacatcttta atattttttt atctgtttct ttgtcctgac gctttcatta 540ttcctgtgat caattttctc

ataccattgg tccatcgtta atcctttctt aatttcattt 600tctacgtaac atgagaggag accaagtcct atgagaacag ttgacgtaac agtggttgtt 660aagttaagtt aaaaagagga agctagtgag agtgaccgtt aggtagagaa gtgagatctt 720taaccactct tctttctctc tctctctgct tttttcgtcg tctttcacat ctactgttcg 780caaactctct tatgcttcca ataatggtga taccaattga gacttgcagg agaatctcct 840cttctccaca ctctatcaac tggtcagcca tggaatggtc gtttcagttt caatattcct 900ggattctttt taaggattcc tgtttctctt ctgttcctgg tatattctta acgacgaaat 960tagtatcgga tcctggtaat acattttgaa gcttttaagt accattgcac tgggatccaa 1020caatgtcctc cgactcgtcc aagatcaaga ggaagcggaa ccgcatccc 1069141687DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0113 as found in Promoter Report #14 14tatgaagaaa ttataataga ctctcataaa aatagtgtta caacttacat tctcttatat 60agaaattagg ataaacagaa atgtaaataa tatatttcga aataatgtta aatttcctaa 120attctaatat taatatttat aaatggtcat ttaacttttt cgtaccggtt cgatgggaca 180tgtgttatat tcagttaagg ttaccaccat gcgccaactt ggcctctacc aagtcaacat 240ggatatggac cttatggtta catgccgcct ccgcctccac cgctaccggg atatggatac 300agaggtccgc cacctcagca accgacgagg aatgaaacaa ggcaataata tattgatgct 360attgtggatt tagttactga taattagtgc cttagtgaca gttcaaaaat gttgttcatc 420aataatctac aatttaaggt ttgtgttgtg gaatgtttca tgattttatg aagtcttgct 480tatcaaaaag tatgatgatt aagaatttga cttcatggca tattcatttg agttagcaaa 540acttttttgt gttgcacctt caaatttata aatttatgat ttttaaccat cgaaattata 600tatttgaaaa gactatctct acaagccaaa cccactgggc caccaatatg ggtttatctg 660cgaaatctgt gaaccttaga aaatcaaagc ccatatccac tttgctggaa ctttgctgga 720atgtaggtta gacaaaacct taagacgcag ctacaagtct cttatgtggc agatgtcaaa 780attaatgagc acgtataatt tacccaagag gagcaaaata agattagcag cttaaattaa 840ttgtgttgga ttaaatgaaa cttgcactat gaatggcaaa aaagaggtta caatctagca 900accacctcat aaaccctcat taatgagata ctgactcgtg aaccaatcaa atctcaagtt 960tcgtagttta aataagtagt aaacacctcc tgatcaaagc atcaccacca ccaaatatca 1020aacgcaaaaa cctattatca aaagaactag ggaggaaatg actaatcccc atgatcatgg 1080ttatgctgtt gttgtttctt gtgatgtcga ctagagcaga cgaagagctg attaagacag 1140agtgtaatca cacagaatac caaaacgtat gcctcttctg tcttgaagcc gatcccaatc 1200tccttcaata tcggaccgtg ctggacttgt caaccatcat taatacactg tctcgggatc 1260tcaacttgat gttcttatca agtaagtttc accatgtaca ccattaatca ttattgtaca 1320aataataata tttttaatgt gttttcacaa aattaatatt acctcttttt tgtaaactaa 1380tatgctacga aaattaacat actacgaaaa atgtgaatta atattacttt gcctgtaaat 1440ttttacctcc ataataatat tagcatacca cgaaaaaatg taacgtattt cttttggtgt 1500aatgtgaatt ttgctacggt aaacttactt tacaaagaaa gaagagcgtt ttccaagtgg 1560aaaataatac attttgcggt ttatatatta taggaacgac tattgatttg tttttttggt 1620tgtaccattg cactgggatc caacaatgtc ctccgactcg tccaagatca agaggaagcg 1680gaaccgc 168715999DNAOryza sativamisc_featureCeres Promoter construct YP0095 as found in Promoter Report #15 15ttcctcgacc atgccgttgc cggaaccggc tagcgcggcc ggccggcggc ggcggggagg 60ccgcagtggg acgacgggtg aaggatcctc cagctgcgga aggaggtggt cctcgaggcc 120gaaggggaga ggctacggag atggagggaa gccgaagaga agggaggctg ctgctgctgc 180tgcatttggg agacgagaac tcgactcgag ccatggcggc agattggtgt ttcacggcgg 240aatgctaact agatccagca tctccatagc aaaggtagaa tggtagattg aggtgagttt 300tttttcccct cttctgcagt tttgatgtat tattactgcc ctcatctgat ctgggtaaca 360tatttctgag ctcagtagaa ctgttaaaaa aaggcagaaa tgcacaaact cttctcacaa 420aacaacatac aaatgcttat attttggagc ggaggcaata catggtatat tttttaaagt 480gaaaaaaaca atcagacaca tggtattgag tgatagcaaa gctgggtgac cacagaaaat 540acctcctgct ttaaatactt tatacctggg ctgtcaatcc tcggagttcc tcccaatgta 600atgtctgagg aagaagtatt gcagctaaat tttaagggtt tcttgtacga aacagggaca 660atcagagatt aagaaactct atgtggaaaa ggccatgcgc attttgttat gtgattcaac 720aaataagatg aggaggcaaa gtcatggttc tgttctaatt aacaaatcta ctatgggggc 780cgttgctccc tattgtccac gctccttttc ttcatttctc tcctgcagga tatcttgtct 840tttgattctt cattttaggt cttataaata tcacgtggtt caggcctcca atgtcaaatt 900atcattacgt ggaactctct tagatgcttg agaaaagtta gctcttacct gtccatagaa 960gctccaagga agcgagaata gtagatactt tggttggcc 999161000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0102 as found in Promoter Report #16 16atttggttga taacgttttc actcgactaa ttatatactt cagaaggata gtaatagaat 60accaaaataa ttaaatgatt ggttagtgcc ttagtggaga ctttttaacc gattctaata 120gactaatgat gtagctaagc atttatttgg gatcatcact gtttgaaaac gtgaaatgtg 180ataaaagtta tgaaacgatt aaaatataaa ataaccgtac aaaacattat gtaccgtttt 240tttctctgtt cttttggcga tttggtttag ttcgttacac tctaaatgtt attgcagata 300tatatataat gatgcatttg catctgagga acatataatt ccggttaaca cttccaaatc 360ttatatccgt ctaggtaggg attttataaa tcatttgtgt catcatgcgt tatgcttgtc 420ggctttgacc ataacgcaga gatatagaac tagcttttac ttaactttta gatttattat 480ttgatctaga gttaagtgga gatatatagt gtttttgtta gattattggt ggatgtgaga 540gtttgtcttt agtttcaagt tgagaatata aggcaagagg agactctgag gcaatcagag 600gttttgattg gcaaaatatc caaaaggccc aaaccaagtc gaagcccatc tcgtacaaaa 660aaagaaagag atctgtaaga aaaaatattc tttgatattc ttacaaaaat aagtgtaaaa 720cttttattag tcaaaatctt caatctttaa aaactctcat cactcctacg aaagcgcgtg 780agagttatga gacattcctt aatagcatta ctcacaagtc acaagttcaa aacgtctgac 840tgaaacagaa acaagccttt gttgaagtct tgaagaagag acattagtac tcgtcgtata 900gccataaaag gtaatatacg aaatttcttc gctaatctct tcaccttcct ctacgcgttt 960cactttcact ttataaatcc aaatctccct tcgaaaacat 1000171004DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0103 as found in Promoter Report #17 17gttttgaaga acaatctgga tcgaaatcta acataaggtc atcgtattca agttacgcag 60tcaaggactt gacatcatcc tactctggtc tgaggttacc acttccaaag atgggatttt 120tcgactcggt atgcttccta agaaattcgt tttattgaac ctagcaaata tcttgtaatg 180taagattcct gagatgatga agaaaaaaca aacttttgtt acagcaggag aacggagaga 240aagaaaacag agaaccaaat gctcttgaag caaacagaag aagaagacac aaatccaaac 300ttgagacttc ttctacacca gaaaaccgca gcattctggg acaacgcaaa acacgaaagt 360gaaacgggca atgatatata tgtcttgggt gcgttacaag gcatcgtttg caactgttga 420gttggataag tcaactgtct tcttttcctt tggttgtagt agctgccttt tttttccttt 480gttgctttaa gaaatagccc gaaaaaaaga atgttctaca tttcggagca gaaaactaac 540cgaatgagtt tttggtcgga tcatcggatc gatcagatat attttgagtt acgaactgtt 600ataaaaaaag ccataatttt gtgttgagtt tgcaaaatac cttataactt gttatttgag 660attgcacctc catatatatt aattcgtaag agtatttatt aagtaagctt tagtataaat 720ccttttttcc tttaaagtaa gttaatgttc tactaaataa tagtaaagtt gaagaaccgc 780tccgttttta caccatgcac gtgttatcta acaaagaaaa tatggtacac ctaatggcta 840atgcaaagga caacacaatg aaactaactt gactctgtgt tatagaaacc catagacatc 900tgcatacatc ctagtatttg tataaattgg actcaaattc ctgaggacaa tcatagcaaa 960caatcacatc atcgcaatat acataaacaa aagaggaaga aaaa 1004181003DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0107 as found in Promoter Report #18 18taacaatcct tgggaacatt gcatccatag atatccggtt aagatcgatc tttgaactca 60taaaaactag tagattggtt ggttggtttc catgtaccag aaggcttacc ctattagttg 120aaagttgaaa ctttgttccc tactcaattc ctagttgtgt aaatgtatgt atatgtaatg 180tgtataaaac gtagtactta aatgactagg agtggttctt gagaccgatg agagatggga 240gcagaactaa agatgatgac ataattaaga acgaatttga aaggctctta ggtttgaatc 300ctattcgaga atgtttttgt caaagatagt ggcgattttg aaccaaagaa aacatttaaa 360aaatcagtat ccggttacgt tcatgcaaat agaaagtggt ctaggatctg attgtaattt 420tagacttaaa gagtctctta agattcaatc ctggctgtgt acaaaactac aaataatcta 480ttttagacta tttgggcctt aactaaactt ccactccatt atttactgag gttagagaat 540agacttgcga ataaacacat tccccgagaa atactcatga tcccataatt agtcggaggg 600tatgccaatc agatctaaga acacacattc cctcaaattt taatgcacat gtaatcatag 660tttagcacaa ttcaaaaata atgtagtatt aaagacagaa atttgtagac ttttttttgg 720cgttaaaaga agactaagtt tatacgtaca ttttatttta agtggaaaac cgaaattttc 780catcgaaata tatgaattta gtatatatat ttctgcaatg tactattttg ctattttggc 840aactttcagt ggactactac tttattacaa tgtgtatgga tgcatgagtt tgagtataca 900catgtctaaa tgcatgcttt gtaaaacgta acggaccaca aaagaggatc catacaaata 960catctcatag cttcctccat tattttccga cacaaacaga gca 1003191071DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0110 as found in Promoter Report #19 19gggatgcggt tccgcttcct cttgatcttg gacgagtcgg aggacattgt tggatcccag 60tgcaatggta atataaaaca agaaaacaag agattttata ggacaatcac taaatgacat 120ttaattgatt aaacatttat tcattaataa ttgtatgtta ctaacttcaa catttaataa 180ttttgtttaa gatacgttta catcagagac tattaatatt tttacaggtt gtaactttaa 240actttgtctt gaatcgaaca tgactataga ttttgggcaa acttaaagat aacaacattt 300ccgttttttt tcaaattatt acaaatcaaa ctgatatatt agacacaaca cgattacacg 360taatgaaaaa agaaaaagat aaaaagataa aagaagggat cgattctgtt tggtctggtt 420tagtgagatt caaagttaag ctcttccttt caagacatgc cttcttaaac cgggaatgtg 480aacgtttgta atgtagtccg tccagttaat gcttccaaca tcaaatccaa attctctctt 540ctcgtcctct gacatattct ccattaatct ctggggtatt gctgttatca aatctgtaaa 600agaaaccaaa aaaaaaagat gaaaactttg cgggtaccgg ttttgtctgc tctaagaatt 660agaatgttaa tgagttctgt cttaccttcc accatagaaa gtgtatggct cataaatagt 720agcaaggtgt ttggcttgtt caacagattt cttgcatata aactttagct tctgcatcat 780cttactatcc actgaactca taccactcat caacccactc cgttcttgag catctctcca 840caaatgatcc gagaaatcat caacggaatt gaaaagtttc atcaaacgca ccataatagg 900atcaccttta gagtccatgc atggagatgt tttgtagtgg ttataaagaa gctccgctaa 960gtcttcgaaa accagcgggt ttatcgccga agaagcgatc tgatacacgt ttatttcagg 1020ttccggatct gacattgcca ttccatgctt tgctatagca gctaacgttc c 1071201453DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0112 as found in Promoter Report #20 20ttatgtgccc tgatgtccta tgcagatggt gcaactactg cttttggtga gaagcttcgc 60gaacaagttg aggaaaggct agaattttat gacaaaggtg ttgccccacg caagaacgtg 120gatgtaatga aggaggtgat agagaatcta aagcaaggta tttcttgtag ctgttttttt 180ttggttgtaa tcagagtcct ctttatgatg gcaaactcag tgttttttta tctgttcctc 240ctttagaaga ggaagggaag gagccagttg atgcctcggt gaagaaaagc aagaagaaga 300aggcaaaggg tgaagaagaa gaagaggtgg tggcaatgga ggaggacaag tcagagaaaa 360agaagaagaa agagaagagg aagatggaga ctgcagagga gaacgagaaa tcagagaaga 420agaagacaaa gaagagtaaa gctggaggag aagaggagac tgatgatggt cacagcacca 480agaagaagaa gaagaagtct aagagcgctg aatagaaagg gatgcaacat taacaaaccc 540tgtattgtat tttttttttg agctaaatta atgtcgtctg tttttcgtag tgaacatcgg 600agaatttttg ttttggtctg gaaacgattc aaggtttggc aatatcttaa gtttgtttag 660gttttcacta ttttgacgtt tgcaaccgtg aaggaggctc ctccatttta taaaatacaa 720ttaccaattc cagtgctttg caaatgtttc aataatagct aaactaacta ccaaattgga 780aaactagctt aacaagtttg tgaaaatgaa tttggagcca tatgatttat tattttaccc 840aaatggagta atagaagaag agcagctcgc gtttgaatgg tcagttaaca ttaacaaaag 900gtaaaattga atagatgtta aaacttgtgt aagtaaacaa tagagctacc tccttttgag 960aaggatagat aaactcgtga ccaaccacat tcccagtccc atattcttag tacaaataag 1020aaattcacac ccctcaaaag aaatataaca taatcaatca taggaaatat acttcgcata 1080atgacgataa tgatcaagtt tctcctgtta gctctgctcg tgatctctcc gatttgcgcc 1140gagaaggacc tgatgaaaga ggaatgccat aatgcacaag ttccgaccat ttgcatgcaa 1200tgtcttgaat ccgacccaac ctccgttcat gcagaccgtg ttggcatcgc cgagatcatc 1260atacactgtc tcgactctcg tctcgatatc atcaccaagc aagttttccc ttctaataac 1320catacatata tattaactta gatatatgac aatattctct aactaatata tcaatctttt 1380tattgttcta ccattgcact gggatccaac aatgtcctcc gactcgtcca agatcaagag 1440gaagcggaac cgc 1453211085DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0116 as found in Promoter Report #21 21aaacgcctct tcggtccacg ctgtcgtttt attgaaggaa ttatatttta ttttaattgg 60gcctgcaggc taaactataa gtccgtctga tatgggtcgg gttgggctta tgagttatgg 120gtctggtagg ggtcaattag cttaatttcg atatgtgccc tactctcgac ctaacgtttt 180gaacacgtaa gagagagttt ctaatattga gttgtctaat taactcgata ggcttataca 240aagtgtttcc gcattttacc ttcttaataa ctcatcattc actaactaag aaaagtttta 300ctcagaccat atcttccgct tcttgattat tgtcaatttg ttgtcactca atttatctct 360tgcaaaattt agttgaaatc atttggtttc atctttggct cttgaatagt tgcatgtgtg 420tatttagtaa gttcttttca attaagaagg aagaataaaa caaattgtgg ccagaaacaa 480ttatgttgag ttttatctca tacgttggct cactcatccc catctctctg cttttgaatc 540attctactcc tcccattttt tgatcgtcct tttttctgct tctgaacatg gatcattgtg 600catgttcgga tgttcctcga tcgtgctgaa actcaaagtc tgaatcgatt accatagact 660ctcaacccat ctttgatata taaaaaagag ccttaaccca tctcttctac tctccctctc 720tagaaacaaa cacatcacgt gatgatctgt ttccccccat acttacggga tgatcagaat 780gtggcatgag gaaaaagcca agaaataagt tgataaattt aaggtttaat ttaacaaaaa 840tgagagatta atcttttcat tttagggtcg cacgcggtgt tttgtgcaac cgcagaaact 900tcctataaat accgatacaa tgtgcatgct ttctacaact caactcactc aaaccaaaaa 960aagaaacatc aaaccccaaa acacacataa caatcacaaa ccattgcact gggatccaac 1020aatgtcctcc gactcgtcca agatcaagag gaagcggaac cgcancccgt ttggaaatca 1080gnccg 1085221100DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0117 as found in Promoter Report #22 22gtcagtgagt cgattggatc acagtccttt atgataaaac aaactcataa ttattccacc 60gacaacatgc gttttaaatt attttttctt aaattatatt atattatatt gatatcaacc 120tagctaaaat aattcggatg gcgaaatcgg acaattttta atagaaaaaa tgggtatgaa 180gatagtctat gattccgttc ttagcgacta gagggacctg ctcaaatctc ccgggtgata 240cgcgatgtca agctcaatag aaccccacaa ccgacgagac cgagaaatcc ttgatttggg 300ctagaagatt ttgaaataaa tttaatatat tctaagtaac ttgcttaaat tttttttcaa 360actctaaaga cataactaac ataaagtaaa aaaaaaaaag ttaatacatg ggaagaaaaa 420aattaaacta atgattagct ctctaacgtg tttaatctcg tatcaagttt ttttttaaaa 480attatattgc tattaaaaca ttgtactatt gtttctattt tgtttagcta ttattcttgt 540gaaatgaaaa gttgtgttta ttcaattact aaatggcaat atttatcttg gaaaactata 600cctctaattg gattaggccc tagacatcct ctttagctta ttgacgttaa aattattccc 660aaaactatta aagtttagta gtttgaaaga tgcatcaaga cctactcaga taggtaaaag 720tagaaaacta cagttagtgt gattatattt taaaatatat aaaacaatct tattaaacta 780aatattcaag atatatactc aaatggaaga taaaaacatt tagtctgtta ccactaccag 840cctagctagt cactaatagt cactttggaa ctgagtagat atttgcatct tgagttacca 900tggactcaaa agtccaaaaa gagaccccga gtgaaaatgc taccaactta ataacaaaga 960agcatttaca gcggtcaaaa agtatctata aatgtttaca caacagtagt cataagcacc 1020attgcactgg gatccaacaa tgtcctccga ctcgtccaag atcaagagga agcggaaccg 1080catcccgtta aacgaaggcg 1100231001DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0118 as found in Promoter Report #23 23aattgagaaa ggtgcctcaa tttcagtaga acctgacgca aaatttcgcg atcatgcatg 60actcaaattg gtttattcac ttaaataaaa aagttgtttc cctatctagt tgaagttctc 120aattcaaacg caacttctta ctttttcttt ttatttatac tggaatgaat ttttcgtcaa 180tgctagacct caatatttgg tgattaagtc caaaaaatta tagcaatatt cattagttaa 240atcataataa tatttgttat ttctgctaaa tatattagtt ttaaattggt aaatatatca 300gtcatcatac tttatatatg tgcacaagaa aaagaggaaa aaaaactaac ttttaataaa 360ttgaacgcta tcctctatat ctcgtcctgg tccaaatgta aacttcaata tccttttgat 420tttattgctg attgctttaa aaaatttcac aaacactttt atcattcttt tattccacca 480aaatctacag acataatact ttgtaatttt atgtaaaaat cttcaaaatt tgggaaaaga 540aaaatcattt aaaatcaatt tgcattaact ggatttattt ccaaaggtgt ggtgttgtgt 600ttatatatgt ggagttgttg gctagtaata taataaggaa aagagtgaaa catatgtagt 660ataacgtatt tctagttttt tttctctgta ttaatgaatc actaattaag tagtatgcat 720taattgaatt atcagaagct ggtcacaaaa gtctaccaaa aaaaacaaaa aaattggtcg 780gaagaaaatg aaaataatga gaataaaaaa gggaaaaaaa ataagaagct agcaaacaaa 840gcaattaaca tttcaaggca gttaattcat catgcaaggt gcttatgtgt gacaacgtca 900tgcgttactt tttgcgtcta cactcatctc tctaacgcaa tccactaatt ctggtaatgg 960attctgctat ttagaccaac cagtttcttc gtctctcaat c 1001241076DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0126 as found in Promoter Report #24 24cattgtatct gagatgtgac tgtgaagaac aaagattcat gacatggtat tgttaagccg 60cccatgtgga tgatcataac caaactctgt cctcagattt actcaacagt gtgtgtgaaa 120caaaggctgg tttaagtatg aaaccggcac cacatatctc ttcttcttct gatcattctc 180tcctacatag accgccatga atcctcttgg tgtcgacgat gattcccttc gaataatttg 240cttagcaccc aagaaactcc tcaaaaaagc catattttcc cttatgtttt cctgaagctt 300aaatgtttct tagtcttgga gaaagctttg agattttaaa attggatctt ctttagtttg 360tgaatctaaa ggggtttagt tacttggtat ataaacgaac gtatgaaaga aatgattaag 420gatttttgag gtttttcttt ttaattacag agcacatggc tttgggttgt agatactaaa 480ccaagaacaa atcaataaat ggtgtctgag aagttagtgt ctaatgatgt cctacatgat 540aacttcattg gggcttattt gtctcaaaga catcacatgc ccaaatctct ctatagatta 600tgtagggaca tgaagttgtg tacctaatga accacaagtc tctatcactg attaagtcat 660accttcttct caatgatatt caaaagacag gaccacatga tttgattata tactgacaaa 720gtcacaaaag ccttcaaaaa aattctgtgg caagaaagga aaatttgact agttatagtg 780tctatctaac aaacaagtgg tcatattgat ttctatcttc acatcagaaa tcatgaagat 840tgatcactat agggccctta cttatcatgc cgtggtccgg caaagccatg tgcttgcttg 900ttggtgtaaa aatttatgag ctgaaacttt tgaaaccaat aaagggttat ctacaagtaa 960tgttcttatc tatatatact catcactgac tcctttctgc tctgccattg cactgggatc 1020caacaatgtc ctccgactcg tccaagatca agaggaagcg gaaccgcatc ccgctc 1076251017DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0127 as found in Promoter Report #25 25tcagtgagtc gattggaacg tttaaagttg agacataacg cagtgatttc aaatttgtat 60tagggtggtc ttattgtgtg tctagctact agctagagaa tactagaaga agaatacgta 120gcaagatacg cataacattt ggtcctctct tttttttact ttcttttaac acattgtcct 180cttatgattt gcttattgat ttcagtatct ttttgtatca ataattccct ccaaatgatt 240aaaccctaaa aaaatgtgat tcattcacca cccgaagatt agcatcatca agtaacacac 300aataactacc aataacctag ttttcatttt tctatactaa aatcctaaac atcccataaa 360aatacaaaca actctgaacc aataatttcc tctaatccac gtgcacccca tcgtctcctg 420acgtaagatt tgtctataac ttatcaaatc ccaaattcag ctttgttttc attatatagt 480acgtactctt ataaaaaaga

gaagagtaca catctttaat actttaactt aaaagaagaa 540agtaatacta atataagagg agtctgagtc agcgacaagt gttcgcggag aaacggaaac 600gctctctttc tctctcttcc cccaacgcca atacctttgg aatccctccc taactctgtc 660ctgtcctttc gtcctcactt tctctctttt tacattttct acacaccaat aaaattgaaa 720ccagcaactt ataaatcaac tcaagtttga attaatgatc gaaaaactag tttatttgtg 780tcaatatgac ccattcttta ttcacataag tattttaact tttcaaaatg ttatctcaat 840ctcctttgag tttctgtctt ccccataata aatttcaaat aattaataca catggttttt 900taattagaaa taatggaaaa gaaaggacaa aggaataaaa aagaaacaca agttggcaca 960ctctctttat tattcactcc cctctataaa tctcatacta tcttctctca tcttctt 1017261004DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0128 as found in Promoter Report #26 26gataaactga taatggaaaa gaacaaagaa accagttttt aactatttgc atatgtaatt 60tatttgttgc aaattatatt tagttaaaat gtttcctcta tttatatata tatatatcag 120tcaagcacta tgtataagaa atgtcaattt ataaattttt acatgtcctt taacagaaag 180aaaatgaatt tttacatgtc attcatagag agtcactcgt ttatttctta tatagagaat 240aacacactca catgcatatg catgcaatat gatacatttt atgacaaaga taatcaacgg 300aaacggtcaa gacataattt gataaacaac ttgcacgatg cacagatctg atcaaatata 360taactcttta acatatccaa aatattcaaa aagaaaaact cgatccaaac tagcaacatc 420acgctcacgc ggtaggctaa aaatttatta atctccaaaa gtctttctta tgaacactgc 480aaacacaaca acttgaaaag tcatataggt ttagatgatg acgcgtattg gctatcgctt 540accggagtgg ctcataaata caataaacaa tacgtaaaag tcaaagtcaa atatatttag 600tcaactataa ccattaatcg ggcaaaacct ttagctgtca aaacaacgtg aaaacgatat 660ttgtatatat catcaagaat cagtagataa gagaatgatt taatcccctg actattacaa 720ttttggtgta ataaacagtc tctattggtt tttattcttt gttttaattt ctcatgacct 780atagagagaa ttaggtagtt tcgaaaattg gctaatcaac ttttgaaaac tactgtctac 840tttgcttaaa ttctctacac ttagtttcgg ataagataat tgtcggacta atagttaatc 900ccttgacaat ctttgatatt ataaaaggtt tagttaatct cttctctata taaatattca 960tacaccagct ttcaaaaata tataatccaa acaccaaaaa caaa 1004271081DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0020 as found in Promoter Report #27 27cagagcagtg catatttttt tttttttttt tttggtgtta gtgcatatct atatatatag 60tactattata atatatttca atatatatat tttaagaaaa tatctgattc ttaagtttgg 120acttatttgt caacaatagc cagtaaaaaa caaaagcgaa gtttcactaa cttaaaaaat 180aaccacattt gtatatttcg aatacatact ataaattaat aaatttatca aaacaactat 240agaaactgtt atttccaatc aatttcttta tcaagattat atctgaaata tatttattaa 300aattaatagt tatttacaag aactattttt atgaaagtgt aagaactctc tgaaaacttg 360ataagtcaat attttttcta acatcgtaaa cataaactag attcaaattc gaatctagtt 420attcaaaaac ttataaaaac ataaaaatga aatactgtta cttcaacaaa aaaacattat 480tattattttg tttaaatatc taatttattc atcaacagca aaatatttaa aagagtggga 540aacaaataaa aattaaactc tgttttggta tgataaaatt atttactaaa ctaaactcaa 600tatattttta gtatcacggt tataactata acaataatcg aactttgtta ttttcttggt 660actggtttta gtagtataga tagatatttt agtcataact cataagatac atgtacaaat 720atttgctata tatgatcagt gataactgaa tttcgtgctg aaaattgcca tagtttgctt 780attttactct tgaaacaata acgatatggt cgttacttaa aacaacattt taaaaacgaa 840gaaaattaaa cagagtttgt taaaataaat taaataccat aaatttctct ttgactcttc 900ctatatagta aaatctctca tccccttctc tctctctctc atagcatgtt ggtctttagg 960ttcctatata aacaacgcca cacacaccca tttagtccca ccattgcact gggatccaac 1020aatgtcctcc gactcgtcca agatcaagag gaagcggaac cgcatcccgt taaacgaagg 1080c 1081281091DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0022 as found in Promoter Report #28 28tagttccatt acaatttcca aatgatttgt tacaaagcta caagattatt cgaaatagga 60tttcatccat aagagagaat ggtgtggtcg acgctacaat gttgatttat tggttgtggt 120ttgcatcttg gggatgtcaa atcctaagtt tcaagttctt gtaaaaacgt tttcaggttt 180ctttaatata ttttaatatt aatgtaaaaa gaaaagatat agcttttgta caaaaaaatt 240tgtttaatca ctatgtagga ggatgcgatc aaattcatgg aatgatgtat tattagcttt 300tctatcctca ctctaaaaac aatactatag tgagttaaat aatttgatca tttcaatgta 360gattaaaatt ttattaaaag aagaaaaatt taaaagccta taacaaaata aaaaaggagg 420ctcgaggtat gatgggtgta gcagaagagc tggcaacagc tatcgactga gtgattacga 480actcagtact cagtgttctc agctcacaca ctcttttttt gttctctttc ttttggacag 540ctttcatttt ctcttttctt ttttctattt tgtttcaaaa ttccatccat attaaaataa 600gcctgatcat gagaataaag gaaatactaa tgatgagttt ctcaataatg caataagatg 660caattattat gagctattta ctattgaaaa tgagcaaata aatgtcaaaa cacaatctgg 720ttaagttaga gcaactccat tgtataggat tcatgtagtt tctaagaaaa caaaatgtat 780taatatttta cttttacatc caaaaaacca aaaaaccaac ttatatgagt aatagaaacg 840atcctcatat taggaatttt agagattttc tctcatctgt ttcttaactt ttcaatattt 900ttattttttt aaattgtatg agtttctact aagaaactac tgctggagtt ggtcttagct 960tcccaatgct tctccaccta tatatatgca tatctccttc ttaaaactcc attgcactgg 1020gatccaacaa tgtcctccga ctcgtccaag atcaagagga agcggaaccg catcccttat 1080actaaaggcg g 1091291082DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0024 as found in Promoter Report #29 29tgttaaggga aggtttgcac ctaagaattt tgaaggaatt ttgcggcgat atatcagtaa 60gtaactttct tcttagtctc aaaatttaag ttgccataaa agtatatcag tttggagttg 120ttaacctctt gttttattat ttctcagctg actacgtcat ttgccttggt tgcaagagcc 180cagacaccat tctctccaag gagaaccgtc tcttctttct gagatgtgaa aaggtataag 240ttaatctaat tagtcctgat cttgatatgc attcctttgt ttctgtttta cagttttact 300ttctgcgcaa caaagtaata aagtattttg tgtgtttgaa tttgctaatg tgattaacga 360gtgggctaca tggtttttgc agtgtggatc tcaacgatct gtggctccga tcaaaacagg 420gtttgttgct cgtgttagtc gcaggaagac ttgagaaatt agaaggtgaa gtgaccttgg 480tatggagttt ggagctattc tactgcttct gtatgagttt atgagttgaa gaaatacttg 540tcttgttttt tttattttgt tttggaatat gattatgact tgacttttaa aatgggatag 600gatcaaaacc ttttactctg tcaggttcat gtggtcacct tgaaggttga tttagtaaat 660ccatggactt cttttttgtg ttaagattat tcttagttca aaattaatag actaatgata 720ttaacgtcca caggcattgc gttcaacatc tcaaattaaa gcgtggaagg ctcagaaagt 780ccaatataca ctatgtttat ctacagttac aatcatacta caaaaaacaa ataatgtata 840cggtttggtc taatatagcc gcatacgatt tagtatttac caacaaaaaa ttggtctcaa 900accaaaccga acaattggta attaacaatt gttcttttgg tcttgaaccg aaccaaaccg 960aactgaacta tattaaccga ccgacttcgt cctttcctcc ccattgcact gggatccaac 1020aatgtcctcc gactcgtcca agatcaagag gaagcggaac cgcatcccnt taaacgaagg 1080cg 1082301031DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0028 as found in Promoter Report #30 30gtcagtgaag tcgattggta gtacttgaaa cacttggttg gtttcatgta tttggcctat 60atataaacaa acatcgtaat tatatacgga tttttttcgg aattttacgc catatctgta 120agtatatata acatgcatgt cgttttcaaa ttcatatgat gaacgatcca cgtaagtgct 180actactccta caatattgca tgagagagat atgtatttat aaattttatt ttgaagaaga 240aataagaggg aaggttactt gggtggatcg atgtgaaaac aaaagaagaa aaagcgaaac 300ccactaagcc attacatgat atcgaccttc ttatcttttt cctctttatt ttatttttct 360catcttcttt ttgtcaggac ttttttctac ttaatgaaac ctccaaacta tctaactaat 420acactcccat gtagaataaa gaaaattata taagatattg ttgatatttt gtaactagaa 480aatatatttg ctctgtaatt tttcgtaagt taaatcaaca ttttaaagta gaaacaaata 540ttactgcaaa aagtaggatc attatttttg tccaaaatct cagttagcta tagggttgta 600gtaaaaacaa aacacattct tgatttgccc caaaaaataa agagagagaa gaatattgtt 660caaaagtggt ctcttctctc tctaattatg ttttcactaa acccaattag attcaaacag 720tctacaaagt ccaaaagata aacatgggac aacaattcga tgcaaaaaat cctcttttca 780tgctcttttt ttattctcta gtcttttaaa ttactaataa aaactcacaa atccaccaaa 840cccattctct acaactcacc ttcatctaga tttacccact cccaccgaga aacacaagaa 900aaaaaatata catatataaa tatacaagac aacacatgat gctgatgcaa tatacacaac 960aaagtattaa atcttagata ttgtgggtct ccctttcttc tattcatttt cttattcatt 1020aaaaaaaaaa a 1031311075DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0030 as found in Promoter Report #31 31aggtcagtga agtcgattgg tacttgcctc atgtgtttgg atacgagatt actgaacgtt 60gtggtgtatt ttatagtcat gggtttgtta attgttatca tgcttgccta cttaactagc 120gtaattatgt ttttttgtac tacctcggaa gtagctattt tgtcgcttat tgacaacgag 180atactttaag atgttccaca tccacgtcgt aatcggttga tcgaatggtg cctaatagat 240caaagttatc ctcaacaaat atcgatgtgt agtatatacg tgaatatata gtagtctctt 300gcatgcatat catatacaac ttaaatactc tttttgtttc aaaataaata atgttttagg 360aaaaagatta ttgtgtcaaa ttaagtgttg gtctattcat ccaaacaaga aagaaaaaaa 420atacgaattt gttttatata tcattgacga acaatgttta gctaataata aataattatt 480tatttataaa aattaaaagt tagatagttt cttaatttag gtgcatataa gttctttaac 540aaaaaaaaca tttaggtgca taagtcttaa atatcaaata ttttggaaca gtaattttat 600gtataacttt tttcgtacct atcttcacac cgcataaatt gccaaagtca accttttgat 660atttcattcc tcacaaaacc atattaattt atacacctca atattgttta atagtattat 720catgttggct ttcgctgaat ttatcaaagt gcaacatgtt ttatcttaca aaaaaataaa 780aagaaattca cgttgtgtga tcttgagagt tgacttttaa atatatcaca acttatataa 840atacgcagca acattccaat ctctcaagaa aatctacagt tcctccaaat aataataccc 900tccctctaag gtttaaaact atacctcatt aacacattaa gaagctagtc attacttcat 960ttctatattt taaataatgt ttattgataa caattgcagg caactaattt tcagcaatca 1020ccattgcact gggatccaac aatgtcctcc gactcgtcca agatcaagag gaagc 1075321106DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0054 as found in Promoter Report #32 32ctgatctcta gtccagtcga ttggagctta ttttgttcta ttctatcgta tttgattctt 60ctttcgtttt ttttttgttt gacttaagaa accgattgtt tatagtagta aacatttgtt 120tttaatgttg ctcgattcca gtgcacatgt ccaggctaga cacttgtcgt tataaaggtt 180gctttggttc aatattgatc cactagagat gttacaacta ttgttgacat ctgagattgt 240gtgataagaa aatatgaaac tggatttagt gaaagttaca atatataatc atacatcata 300gataggaaat aaggaaatgt cagatatact tgaagaatac atcaaataga caaggtcctt 360tttcttattg tcgactatta tagagccgta cagaaccttt tcacgtcttt agtaattagt 420acattctcca tttcggctct ctcttatttt ttttccatct ctttttactt ctccaaataa 480taacaataaa agcttcgatt ttgtgtgtgt ttgtatttac atcttgacat cgatattctt 540ttcatcaatt ttttaccaaa aatgtaataa aaacaaaaaa aaaccaacgc tgaacacaga 600catggtttct ccatccgttt atattcatcg tttgtatgtt tacttaacaa cttatttcaa 660aatagtacat atcatggttg tgtttttaaa aaaagtatac agaacagaaa agcacatggt 720agacaaaata atgaagccaa aattaataca aagaagaagt tcaacttgta tttattaaca 780cattttcttt ccttgtcaaa gacatgcaaa ttggttttgt tttcttattc ccattttttt 840tttataataa aaagaagaag agtaaaacaa aaaaactatc atttcttctt atcgcaaaac 900tcttatctaa gcaagaaacc gacaaaacct atatctacat atattctcat caacatctct 960tgagacatat tcattttggt taaagcaaaa gattttaaga gagaaagggg gagaagtgag 1020agagaccatt gcactgggat ccaacaatgt cctccgactc gtccaagatc aagaggaagc 1080ggaaccgcat cccnttaacg aaggcg 1106331017DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0025 as found in Promoter Report #33 33gtcagtgaag tcgattgggt ctcaccatga gccaatgaaa agaaatagta atccaggtga 60ttgtttctct ttgatgtctc actttctaat agctagtctc taaaagaacc tttgttttag 120tgaattctaa tatagtaggg gttttgcagc gcaattttct tcagcaccga agaaaagtag 180attgaagatc ggagagtcct ctgctttctt tacatatgtc aaatctactg tccttagaac 240taacggtcag gatcctcctc ttgtcgatgg aaatggctca cttcatcttc atcggggttt 300ggcggagaag tttcaagtgg tggctagtga agggatcaac aacaccaaac aagcacgcag 360agcaacacca aaatctactg tccttagaac taacggtcag gatcctcctc ttgtcaatgg 420aaatggctca catcatcttc atcggggtgc ggcggaaaag tttcaagtgg tggctagtga 480agggatcaac aacaccaaac aagcacacag aagtagaggg accgagcaat accattctca 540aggagagacc ttgcagaatg gcgccagcta tccacattcc cttgagcggt cacgcacgct 600tcccacatca atggaatctc atggtaggaa ctaccaagag ggcaatatga atattcccca 660agttgctatg aacagaagta aagattcgtc tcaagttgat ggatcgggtt tctctgcacc 720aaatgcctat ccttactata tgcatggggt catgaaccaa gttatgatgc aatcagcagc 780catgatgcct caatatggtc atcaaattcc tcattgccaa ccaaatcatc cgaatggaat 840gacgggatat ccttactacc accacccaat gaacacatct ttgcagcata gtcagatgtc 900tttacagaat ggtcagatgt ctatggttca tcattcttgg tcaccggcag gaaatccgcc 960ttctaatgag gtgagggtaa ataaacttga cagaagagag gaagctctgc tgaaatt 1017341026DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0050 as found in Promoter Report #34 34aatctgatct ctagtccagt cgattggtac ttgagggaaa catcatattt ttaaaccttg 60tctcagtaag ctaacacaca ccccttgtga ttacttatcc atgtttatcc acaagaatgc 120agttggattg agatattttc ttctttgttg aaatcaggcc tcaaggtgtt catgtggtct 180gcaaaaaaat tcccaaaaat aaagatagtg acatctgaaa tcgataatgg attagacgaa 240gagtttcgtg ttattccttg gtatgggcgg gtttggggac agatattttg gcacagacga 300ggactaggcc actgtggtcc tgcagcatta ggtgtccctt ccatgtcctg cattacattt 360tattgatgga ttcatcaccc tatctactac aacggctaca caaactatga agagttttgt 420ttactaataa atgcccaagt gaggggtcga tcgaacccgg gacacgtttt tcagtttacc 480atatagaatt atccttggaa cccttgatac tccatagaac atcaccacct ctgttgtcat 540ctcaggaatc caggttcaaa cctagtctct ctctccctag tgggaggtat atggccactg 600ggccaatgat gacaaaatgc aaaaaaaata aaatacattt gggttcatta tctaaaatat 660ctcttgtgtt tgtaagtttt ggttgcacac tcgtgtggtt gaagtgtgtg tgagaggtac 720tatacaatac actctgcttt tgttttgtac ctatctcttt ctcttctcca catatccaag 780actttgggga taaagctgag atcattggtt gccatttggt tgtgtagaag caatcaccca 840tttgctttat ccgaggttga taaatttcct cgggttctcc ttctgacacg tatgacaaat 900tctaatagta tattcctcgt agatattacc tatatattct caatagttgc aggtacttaa 960ggctttgtct tggcatcctc gtcctcttca gcaaaactcg tctctcttgc actccaaaaa 1020gcaacc 1026351087DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0040 as found in Promoter Report #35 35cccatcacat gtaacatcat tgggctatcc aaaagtctaa ccaataatgt caatctataa 60accacattaa gtagttcatt ttttttgtag tcgtgtttag cttgttaaac ctcataaaat 120atgttttcac ttacgttaac aaaacaaata tcttcacgaa aaaaaataaa ataaaatatc 180tttttgatac cgaaaaaata aaataaaata aaataatttt ccctttcgat cataaaatgc 240gtagataaga gaaactgtgt ttgaggctcc atttcatgtt cacctaccag tctaccacgt 300catttctcaa agacgcaaat tttctaatta gggatgtgct ctttttacat atagatcaat 360atcctaaaaa aatttaagat attcatattt tcgtacatat atatcgagtt tcccgaaaaa 420tccataaaaa gggtataatg atagtccttt ttctccttta ataataattt ctgaacaaaa 480ttatatcata ataaacttgt gattttatac aaaatttatt tgtatatata attttactaa 540acaacgtgaa cgataaaaat aatattctca taaaatgttg attaaaaatt acttaaaata 600aataattatt taggattatg tattagtagt actcgaacca tttttttagt tatctgcatg 660aagaccctaa tttttcacat atatcgaaac taaaactttg gatatacact gtaatttgaa 720aacgcttgga acggataatg tagttacctc acaagatttt gtacatccct gacattttat 780attcattaaa gtgtgttttt ttcttcagaa aagaaaacac tttttctttt tgtgctttta 840gtttaaatta acaaaaaaat ggacaccatg agattccact aactcatgtg tatataacat 900tagggaagca gtcaattcat ttcagcatcc acacacactt tgaatgctca atcaaagctt 960cttcatagtt aaacttcctc acaacgtcaa aactcgagaa gaagaccatt gcactgggat 1020ccaacaatgt cctccgactc gtccaagatc aagaggaagc ggaaccgcat ccctttnaac 1080gaaggcg 1087361071DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0041 as found in Promoter Report #36 36gggatgcggt tccgcttcct cttgatcttg gacgagtcgg aggacattgt tggatcccag 60tgcaatggtg caacatcgaa tccaaattct cttaacatca taacacagac taaaatttca 120ggctttcgtg ttaaaagaga gaatatcatt gaactttttc tcttaccttc atgattcctg 180caagaacagc acctccgagg tataccatgt gtttccttct cggtggatct tcgatccgca 240atctgagttt ctgattgata agaaaaacga agataaatgt taaaagctga gctaatgact 300gacagtgatc atggcattat cagaattatc ttttttacct tcaagccatc tttgtttcct 360tttagaactg tgtcgagata ccgatcctgt atttctttct caagactaga aacaaacaga 420gaataaaaga cattgaaaca taaaacaatg gaacttcttg ctgggtttat gataaacaaa 480aaatatacga accggctagg taatcctggg tacatggtgc tacctccgct taaaactatg 540tgttggtaga gctgtgaaaa aagagcaaag ggtttaagac agatacatga ttagttttgt 600tggatctgtc ttttgaaata ccaagcaagc gtaagagtca cacgtaccat catgcggtta 660tcaatatcca tttcttgaat acatcggaaa accatgtctg ccattccatc accttcaaca 720tcaatgagtt cctgaaaaaa tatttggttg tgatcagcct cgagattctt ggagggttga 780aactattcca aaagagaaat tagcagagga cgcgatgaag gtttaccgga gtaaaaagcg 840cttcaggtgc ttggaatctt tcagtgccta ctttgataac cctcccatct ggaagctgat 900cacagttcaa aaacacaacg ggttagtact ctggaaaaaa aataatttgg tgataatatc 960agctttagag gctgcaactg gcacaagtat gaaactttat cgactatcgg atgaaaaatc 1020ccaaacacag cgcccaagaa cagagaaacg atgtcatgaa aagaaaagat g 107137999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0056 as found in Promoter Report #37 37ataggaatct gcttcggtag aagattcgag agaggagagg aagcatcggt ggttttggag 60ttccttattc ttctcttctt tccaaagttt tgtcattcgc caagattcct taaaaacttg 120ttcacacatc ataattatgc accaataggt tataaatcat aatccaacaa gttagtcatt 180ggctttaatt ttaaaaaatc ccataagagt aaaatctttt agaaagttaa tcaacccaca 240catgggctag aaaaccaaaa accccacgaa cattgagatt acaagaaaca tttttaagtc 300ctaaatgagc ccaagagcat tgcttaatga agaagaactg atattaatta actaatatta 360ggacacataa aaaaatacga aaacaccaat cttcatgcca caaaatcaaa caaaaacgaa 420aaaatcaatt ttcatgaaat ggataaagag agagcgtaat tatcaggaat ttgattgagt 480acggttgtta tgatgatcat tcacaattat ctttgatctt gagatttagc aatagttaat 540tttcggatgt ttttttgtta cttgctgctc acttcttgta tgcagattaa tttataagag 600agaccagtta caactctttc ttatttgaat aagattttat aagatgtagt gtggccatgt 660gggtttattg catgcagctc tctgcgttgg tcccaagtcc acgacaatag agagtttctg 720cacttcacgg tatcgtcgtc gtcacaagtt ctttacctta tcattggcac aagttagcca 780ccgtctttgc gcaagttagc atgttgtgct acatacgtgt catgaactga ttggtcaaat 840ttggatatat tttattcccg tcggttatgt ttggataaaa atataaaacg gaaatttctg 900tttcagcctt ccttggtccc aaagaaaaat acgcacacct actcccttca ttctctatcc 960tctccactca taatatatac atctaaatgc aatctctcc 999381080DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0068 as found in Promoter Report #38 38aaattgggga gtggggagat gtttggttat attcccttct catcgatggt ctagatgtgc 60gaggtgactc tcatggaggt aaagaacaat ggtgattttg tgaagaaccc aacgtaatgg

120taattcctaa aaaggttaga agttttttca gcttgttgta ttgctaaaat ggggttgatg 180tactcaacga catccaagtg tacttgagtg agcttttttg gggttgagta cctcgaccca 240ttattcaaac taatgtaaat ggtgaatgca gcagtgactt tgttgccttt tgcaagaact 300aaagaagaca gaaacaggtt gtaaaagaga gccaagtgtg tgtttatggt agaaagagca 360aagtgaacga aaggtgtacc tttttgactt gttgtcactg gttttctccc acttcatccg 420tttcatgctg catcagaaaa caacataaga aatgaatgac gtaacgcgaa gcattaggag 480ttgcttgtaa attaatacat tgccattact aacgtaattc agtagattct aactacaaat 540gaagtcaatg tatctatctg tctactttag ccaatgtatg ataagaccaa atagtcttct 600cttttttcag aaactctcta ggattaaaaa gtttgtgggt gaaagaaata ttatcgtgtg 660gatgataaga ataattgatc ttgtgttagt aaattaggaa tagatataca agtaggtttc 720tctctaaata aaaaataaaa gagtttaaat tgcatgcgta taaaagaaaa aagtaagaag 780aaaatatgtt ccggttaatg gttgggtgca tccgaatcga accggcgcaa accaaaaaat 840ctaaaggaga tttgaggtga taaaaggaaa tcagacattg aaccaaaaaa acaaaagcga 900gacggtggaa agaaaaaact ggaaaagaca gttttagccc ctcctaaaag caaagaaaaa 960aaagataata aatagcttcg tcgtcgtgat cgacctctgc cattgcactg ggatccaaca 1020atgtcctccg actcgtccaa gatcaagagg aagcggaacc gcatcccgtt aaacgaaggc 1080391070DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0082 as found in Promoter Report #39 39aagggatgcg gttccgcttc ctcttgatct tggacgagtc ggaggacatt gttggatccc 60agtgcaatgg tttgtagaga aacaggaagc tttgttgaga aagttttaac agtgagttta 120gtatttatct gagaactgtg tgcgacgaaa ctggagacag gggaggacta tatacaggag 180ttaagtgttg aaggtgggat attgtcagtg agtgaatgtt ctggagaagt ggggagattg 240atatttctct ggatgtttct agagatccgt ggctcccaag ctaagtgaaa caaatataaa 300ttgtcttgtt tattaacggg ctacggccca tcggtattag gcctgtttta gtagatttat 360ggtcaacaag tcaaatgacc ctagaacatg gtacatgaat gaaaaggacc aaaatgtgga 420cagaaatcaa ccaaaggccg aggcggccac caaccaccga acgtgggaag gtctggttcg 480ggcaaacatt cgaaaagacc aagaagactc ggataaacaa ttaaaaaaat cagatattgg 540ggaaggcaaa tatatattgg tgttgaggtc aagacttaga gttttgacgt tattttaaaa 600aaaatatttt tgggtaaaga ccaaagagtt tgacgctatc ttgaggttta tttattcaac 660gtagactata tactatgggg ccgttctgaa ttggaccttt taagttttga ttttattaaa 720acgccatgtt gtttagtcga gagataaaca aaagacgtgt ctctagctaa gctgaccttc 780atatatgctt tgacataata cattaattat aatctctctt tttggtgtgt gtaaaaaata 840cactctatat tttattatgt agacacacta catgcagaag tgttcttacc tctgcctcta 900cgtgctgtca actcctacag atcttcaagt accccaaccg ttatctaaca gattaaatat 960aatatacccc taactatatt atatatcaca attgttcaaa ccagtgtata tagactagac 1020gattccttga accatataat tccagcttta ctataagagt cttaaggaca 107040999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0019 as found in Promoter Report #40 40ttacgcggcg ctacactctt atcaaagttt gaagattttt caagagacac aacagattca 60agattttctg gtggctaaac ttacaatgac agtacatgga ggatctccgc gaatggaytt 120ctgcaatgta ctagcgtaga acaaacactt tttgttaaag tcatcaacca acatagcata 180gagttgttta tctgaacaga acactgaaag tcttggtttt gtttgtgttc cagtaaactg 240tgtcaaaatg aaagaaaata cttattaaca agttcggcaa aaaaaattca aacttttgtg 300cattattata tgaaagcact tctagaaagc taccttcttc ctgctcctcc tgttcctagt 360tttcggactc tccactcgag tgttccctct cgcttcaatc acaaacggct ttactacaga 420catagctgat aaaagggtcg aaaaatcatg aaccaagtaa gcgaaacaga ggataataaa 480catggaagaa gaacagagta agacgaatta taccactcac ttgttattcg aattggaaac 540tggggataag gtttcaaacg agttccgaga atgtcagaga ctctaaactg aacagtagaa 600agagaagtca aagcagccat gccaagtatc attcgtaaag catcgaaagt cagaacatta 660ccctcagcgg aatttaatca aacaccttct gtgcaggaat aatctctggg ggttttatca 720acactcaaaa aaaactggaa ctttgtaaat aaaattataa atgttcgtac ctttatgcaa 780aatttctcac agcgtaatta tctatttcct ttttgtcctt tatgaaagag gataaggttt 840ttaaataata aatactaaat tgtttttaaa agaaactaaa aataaatgga aagccttaag 900cgtcgtcaat ggttctagag tcttctgcaa ctttcttttc atgaaactac tgtaatcttc 960tgctaacata tataatctca aacactatct tctccaatt 99941999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0084 as found in Promoter Report #41 41gatataagta gaatcatttt ttgccgccgt ttctcgctaa cacaccgaaa actgaatcaa 60atctcctagc tcttctacgc aaaatcgagt gcatcgacaa tggcggaacg tggtgtcgaa 120cgtggtggag atcgcggcga tttcggacgt ggattcggtg gtcgcggcgg tggaagaggt 180ggtccgagag gtcgtggtcg ccgtgcaggt cgtgctccag aggaggagaa atgggtgcca 240gtgactaagc ttggtcgtct cgtaaaggaa ggtaagatca caaagattga gcagatctac 300ctccattctc tcccagtcaa ggagtaccag atcatagatt tactcgtcgg tccttcattg 360aaagacgaag tgatgaaaat catgccggtt caaaaacaaa ccagagccgg tcagagaacg 420agattcaagg ccttcatcgt cgtcggagat agtaacggtc acgtcggatt aggagtcaaa 480tgctccaagg aagttgcgac ggcgatcaga ggcgcgatca ttctcgcgaa attgtctgtg 540gttccgatac gaagaggtta ttggggtaac aagattggaa aaccacatac ggttccgtgt 600aaggtaaccg ggaaatgtgg atctgttact gtacgtatgg ttccagctcc gagaggttct 660ggtattgtgg cggctagagt tcctaagaag gttcttcaat tcgctggaat tgatgatgtc 720tttacttctt ctagaggatc caccaaaact cttggaaact tcgtcaaggt atgtactttc 780acaatggctg ttttggtttg atgaactctg aattaggcag tgaaaaagta atcattacca 840gttaagtgaa tttatattga agattaggat ttagctgatt gtattggttt gagcatgtga 900gtttgtgttt aagattgctt gaattgaaat gctttaggtt gtttgattac gctaaattct 960gactaatgta attcaaattg ttgttgtttt tttttggtc 999421015DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0087 as found in Promoter Report #42 42tgaattgagt aaaatgtgtt ttcaaacagt taggtggtag aaggtaaagg taataacatc 60atgatcttac taaaagaatt gttgcatact aactatcaat attctcaaca acataatata 120atgttttttt aggtaatttt ccattttaat tttttgtgat taaacaatta aacaactcga 180atgatgatga taaaaaaaaa aaattaacaa ctcgaataag ttaaagtagc aatacacatg 240tcgttcaatt caaccaataa agtaagactt atatttttaa gaagttgact aatagcttaa 300taagttggaa aacttgtgta gtttcttaat tcccacgtgc agtaagaaat aaaaatgaaa 360aaaattatta tatccttccc actctgcgac ttttctttta ttttatcaaa tattaaaaag 420attcaaaaat agataaactc atatcacagt ttacacattg aaatcataaa cgataattat 480gtattttgta ataaaaagtt agttctgaag ctcatacttt ggatagtcgc tagtcgctaa 540tatgctcctt gtaataatta aagtcactac gacgcacgtc aaagccgata tttagggctt 600aattgatgcg tgtttttctt ttcatataat agtaatataa attagtacta ataaagtatg 660atggatggtt gagacagaaa agaaaaaaga tgactgtatg gtcatcatta caaagaagaa 720tgtattcttc atgttcttaa gaataataaa atgtcacttg taaatcaagt tggtaagcat 780tttgagaact ttgttcgatg caacgtatga tgatttatgt agacaaaaga taaaaccgta 840tcttcaacta ttgccaagaa aagataaaac ctaatctagt cagtctctca acataaatac 900aacccaatag ccaaactgtg tccaattcgg agagaaacta aactaaaaca aaacacaaaa 960gcccaacata agcccaataa aacccatttt ataaacagaa cattactaac actca 1015431091DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0180 as found in Promoter Report #43 43caatctgatc tctagtgcca gtcgattggt tattgttgaa acggatggta tccagattca 60tagagttata cgttgttgac ctcgtacagg atgaattcat tatcttcttc ttcttttgca 120gcatggcagg tgatcgatgg gtatgacttg tgatgatagc catgtccacc aaatcagcca 180agaaaagatc aagacctcgg ctgcttacgt tctgttctat aaacgccttg tagactaaag 240aaactgaagc ggaaaagaca agaaagaggt atttgcattt ttgccgggtt tggcttattt 300aaaaacatca ttggcttgat tctaattcac tacaagatca agatgaaagc agctctgcgt 360tgaggctaat ttacagaaga gagagagaga gttgggaaga agagcaaaag accgagagga 420catgttgcgg ggaatttatt ttattcttac aaaaattggt atctgattat tttattaacc 480atattcaatt agagaataga agaatagaga aaagcccttt tgtgggatat ggttctaaat 540tgttgtttag ttcttgtgtg tcagttttgg ctctcgtcga ccaaagaaga ttaaagaaac 600ctctacctta ttttaactca attcttttgt ttttgcaatg tcctttgctt tccaaaattg 660ttagtcttac ttttcactac tttgatagac attgcctttg cgtttccctg attaataagc 720cagagtactt aaatcaaaat tgactgtttt gtgcatcctg catcacgttt ccaatcagaa 780ccatagtgtt gtcgttgtgt cattatccga atttaagtgg agacattggt aagttattta 840taaactaatt acaatctatt tttctaatta tttcaaataa catatttaag ctctgtagct 900tccactagac ggtgaagatt tgaagtgaga gctctctttg cattgctcac ccaccaatgg 960atctacctac ccttcttctt cttctcctcc ttttaaaccc taaaagtttc cctttccgtt 1020caacaacgcc acaatccatt gcactgggat ccaacaatgt cctccgactc gtccaagatc 1080aagaggaagc g 109144999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0186 as found in Promoter Report #44 44tggacaatta ctcttgtgtg tatccttgga gttgctgttt catatgtaag tggacaatta 60ctcttgtgtg tagccttgga gtttttttat ttacgttatt ttggtcagcc tttaattatt 120ttgcaaaaaa tgtatctgtt tttgccacat gcccacataa tacatttcgc aaatttgata 180cattatgctt tggcccttgt atattcggta aaaaaaaaag ctcaggctac tctcaaaacc 240ggctctgagt attcgtaggc cacaatcgaa gaaaaaaagt gccgatttac atatttttca 300tacaaaaaat taaaactgtt atgtattatt caaaagctat ttacatatgt tttactaaca 360cgttttcaat attttcttaa tccttttcaa aatttaacta agtataatac tttttttgtg 420tgttatttcg ttgttttggt taaagaaaaa cgaaaaaaag agagagttat tcatccttgc 480agataaggct agggttggtt gaataaagat gtgcatatct tataccacta gaccaaagaa 540acagtcacaa gtaaaaggcc gaatcctttt tataaaatat aaacagacga aagctaatgc 600ttcatgggct tggcccaagt gcaggctctc gctagtcgct acgctacaac tatcccatat 660ttaattagtg aagagtattt tattattttg gtcaacgggc tatctttgtt gacaaaacta 720tcccattggt aaagaaatag caaaataggc gtttcattct ctatatttaa acttgatttt 780atgaagagtt gaatagctga accaggaaga tatttaagaa gcccgtactt cacgctttaa 840ctgtcaatcg atagatcata ataaatgact atctatggat aggaactata actgaattca 900gaaagaatct actactacta taaatactaa aagagtatta atacaacgga aaaaacaaaa 960caaaaaaaag ggggaacaag ggagtttcat gttaaaaag 99945999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0121 as found in Promoter Report #45 45ttggattttt tttttgttga gtcagcagac catctaatct ctctttttcc accacagcct 60gctttctatg aagcatttgg gcttacggtt gtggaatcaa tgacttgtgc actcccaacg 120tttgctacct gtcatggtgg acccgcagag attatcgaaa acggagtttc tgggttccac 180attgacccat atcatccaga ccaggttgca gctaccttgg tcagcttctt tgagacctgt 240aacaccaatc caaatcattg ggttaaaatc tctgaaggag ggctcaagcg aatctatgaa 300aggttggccc attctccttg acaggcttaa caatacaact tgtatcgctt caacaagatg 360atggcttaat aaggattttt gcatgtatag gtacacatgg aagaagtact cagagagact 420gcttaccctg gctggagtct atgcattctg gaaacatgtg tctaagctcg aaaggagaga 480aacacgacgt tacctagaga tgttttactc attgaaattt cgtgatttgg ttagtgtaac 540ccactgttat tcttttgatg tctacatcta ctttacttac attattcttt tcttcggttt 600gcaggccaat tcaatcccgc tggcaacaga tgagaactga tcatgacagg gtaggatttt 660atttcctgca ctttctttag atcttttgtt tgtgttatct tgaataaaaa ttgttgggtt 720ttgtttcctt cagtggtttg attttggact tatttgtgtt aatgttgttt tggctgttct 780cttaatatca ataacaaata aatttactgg ttggtatcta agatctaaca atagttacta 840tttttagagg taaagacacc aaccttgtta tattggtcag agagctaaaa ccttgacttg 900ttgggaaaac aaaactctaa tgacagaaaa tctgacatga tgccttataa ttcacagcct 960catgttctac ataaatccta acaatagcac tttgtttct 999461020DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0096 as found in Promoter Report #46 46gaggtcagtg agtcgattgg tgcaaaattg aaaaattgaa gggtgaaaca aatttaaaga 60taatatctat taaatcctct aattttaaaa atttagcaaa aattgtattt tcttatggat 120ctgttagttc acacgtatct taattagtac caaatcatat ctaatgatta gtgataaaac 180tagttagata tctatatgtg tctttaccat ttaacttgaa tccttcttct tttttttacg 240taaacaactt gaatccttcg ttaatacata aatttaaagc attttttctt taattctatt 300gatcggtata tatttactat aagttttagc tcatatgcaa tttcaaatga tatgctttta 360aattttgtct aggtgtgata gttgtatctt taacataaat cttatagcaa aattatactt 420gatattctaa atttatctat ttgctcttgt gaacctcata ttagtctaga gaaactttga 480aatcctttca attagttgta tgtccaatac atttttacta acatttatta gtctttttaa 540ttaagattat tgttagaaaa aaaaagattt tttaaaaata aataatatgt tttagataca 600atgtgagtta ggcttcttat attttaaaaa ataaatttat ttcatactta aaaatagttt 660ggaatttcaa tttatttggc tgaataccat aaaatatgtc aatttgaacc ttatacccat 720tgactatttg gtgttagaaa ccctttaaca aaaaaaaact atttggtgtt agatatcaaa 780ataaaaaaag tttaaccatt ggtttcttat attgaattgg atattgttac atgtattaaa 840gtttttttgg tttaattttg aaacgttgat agaaactatt aagtttaagt ttggtagtat 900atttatttgt ggaaaattta attgccatta aatataacgt caactttttt tggttttttt 960tgagaagtta cgttgtgatt ttgatttcct atataaaagt tagattacgt cattttttaa 102047998DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0098 as found in Promoter Report #47 47tatttttata aattatctta gtaaaagtat gtattttcta atagatctgt tagttcatac 60atatcttaat tagtgttaaa ttagatctaa tgattagtga taaagttttt agatatcgat 120ataggtgtct ttaccattta acttgaatcc tttgttaatg taaaatttta aaatattttg 180ctttgattct acttattggt atataatttt aacatatcaa tccaatgcca ctcttaaatt 240atcatgtact tttcgatata tgttatgact cacttgttat gaaacaatgg attttcacca 300attttggtta tttattaact agaagtttta gctcttgtgc aattttaaat gatatgcttt 360taaaattggt ctagttataa tagttgtatc tataacataa aacttataac aaaactatac 420ttgatattca aaaattattg atttgctctt gtgaacttca tattagccta gagaactttg 480aaaacctttc aataaattgt atgtcgaata aagttttaca aacatttatt agccatttcg 540attaagacta ttgtgagcaa aagttttttt tattataaaa taaataatat gtttaagata 600aattgtgagt taggcttctt atattttaaa aattatataa gtttatactg aaaaagagtt 660aaaattttca aattttaaat ttatttggct taagaacata aatatgtcaa tttgaacctt 720atacccacta aatattccat gttagataac gaaataaaag aaaattaact attggtttct 780tatattgaat tggatattgt tacttgtatt tatgtttttt gtttcatttt taaacgttga 840taaaatcatt aaactaaagt tttgtagtat atttatttgt cgaaaattta ttcccattaa 900atataacgtt aaatttattt gtctttatta aaaaagttac tttgtgattt tgatttccta 960tataaaattt agataacttc aattttcaaa taaaaaat 99848999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0108 as found in Promoter Report #48 48ttagctgaac caggaaattg atctcttata ccagtttccg ggtttagatt ggtttgatgg 60cgatttgatt aaacccccga aattttatgt cgtagttgtg catagtatta ttattctttg 120cggacaatag acgtatcggg accaagttct gtagcaaaat tgtataagct taagtttgat 180gaaatttaaa ggtaatcact aaaacccaaa tgggacaata aaccggtgaa gatttagagt 240ttttaatttt gactcatgaa tctggagaaa gagccctcgt taaaaggagt gaatcaatcc 300ataggggaaa aagttttgtc tttttaaaaa ctaaagaacc aaaccttaat agaagcagct 360caatgtgtga caactttcca ctggcactaa gataaagtga ctagcgatga gtgcaattat 420tgaaatagta gatggtaaat attacataca agagtaaaaa tatctttatg tcaatgctta 480attcagtgtt tctggttaac aagagaatct tctctaactt tcgtaattgg gtcttataaa 540attttatgca attatgattt taccctttta ctacttttca ttagctttca cgaatctatt 600ttgacaagag aaatcattag aggtaaacat gctttttggt caagggcctt aacagttcca 660ccaatcaagc tcaaaagttg tacttaaccg acatcttctg tgaaaacata taattacatg 720tacaaatcaa aactacctta tgaaataaat agaaatattg cagttcattt ctaatttaac 780ctcttcaact tttaaaacta tttacatttc tttatgtcat ttctagtcat tttgatgcaa 840attgtaccat ttatggatta tcttcacaaa tttttaagtt ggtgaaaact ttttggtggg 900tagttaaaac ttgaaataga aatttacttt accaaaataa actaatgaaa agtaatcact 960ccactcccta taataagatt tccaacgttc ccactaagc 999491001DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0134 as found in Promoter Report #49 49cctactttag gcttaaacaa gaagaaaata tgactgctaa gtcatatttt tcaactctca 60tgagcaaccg taaagttgca ccgcaatatc caacaaatga cattcgtgtt atctacaatc 120taatgttgaa aatttggctc atctaataaa ggagacaaaa gttatatctc tttcacacac 180acgttaatgg aagtgtaaag gcggtgagag tgtgggagag acttggggaa caagaagaag 240gacgcggtca aaaagtgacg gtgggctacg gcttttcttg gtagcagttg gaaattccat 300taatgactta aaaagtgtaa atcttatctt ctttttattt tgtgatttga tatgcacatt 360catttcatga aaatatttgt atagtttgat gatcatacga caaacttata gggttcacaa 420agtagatgca atagttgcat acctctgttt aaatgttctt gttaatatta taattgatga 480cggaactcgt gaatgttatt caaaatgtcc atgtaattca agatcatgca ctataataag 540taatctatca ttttcagcac aacaattttg acaaaaagta aaaataaaat agagtaaact 600gatatcatat ttccgaatta tatatataaa cgttttctgt ttctcaatgg tctctttcac 660tcttgtgttt tctaatattt catttaaacc tatttctaaa ctaagcacat ctttgttgat 720tgattgcatt tcaaccaaaa tcgataaccg aatcattgtt tttttatgtt ttatttcagc 780ttaccacaca cgtttagaat tttaaaaata aaacaaaaaa aagttaactc gttacaaatg 840aaaatgatat ttttaattgg actcgatgga aaggaccaat ttattcaaca ctattgttta 900gtccgaacac ttgccgcgta agttttccaa ctccccccat tgacctttcg cactttcaca 960aactccgtat atatataatg gatacactct ctctttgatc t 100150999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0138 as found in Promoter Report #50 50tgtgtgtcct aaatagtttc tttttaaaat ttgtaaatac caagacgcgt atttaagagt 60attttgaaaa gatatttgat tataaaaaga aagaaaaaga gaaggctgag gattaactgc 120aacgtctacc gttggaaaag aaaaacgatc agaaaacaca gaaattaata aaaagagaga 180aaaaaaaata gagtatgaga gatgcacatg ggtgcctgca aaaaaaaggt agaagaaatt 240tgtctgaaag tgtcacaggc acactctctc gaaccacatt taacaacact ccaaacactc 300ttcttctact ttgtaccctt cagtacatta ctctttccaa agtccgtgat ttacgctctt 360cgatgacacc tctcaacaga gagagactac atgtgtacat tttcttctac cattaaattt 420tgaagatttt cgatgattca atttagtata tatatggaag ataaaatttt cattgtcttt 480ctacatgata gtaacggttt tagaagggtg gttatcactt atagtatttg agttaagaaa 540tataaaaata tacgtgactg tttttccttg taaactattt ttaggccctt atttttattc 600aagtagtcac atacgtgttt gaagtgtatt taactaagaa aaagaaagta ggaaatgaaa 660aggatatagt atttatggtg taatcttggt aaggaccagg agatcagaag gggccacaat 720gtcacaaaga ggaccaacaa tgaaattaaa tcctcagctg gcctttaaca ttttggctcc 780caccatctcc ttccacacat atgcacatgt cttcatgtct ctctctctct atacgttacc 840tacacaaata tgtacagaca aatagcccat tacaaaatct ttatttataa atatatactc 900ctcaactccc tcaatatcca cccatctcct tctccataac tctctctctc tctccctaaa 960cacaaccaaa gacttttatc tctcaggaac cccaaaaac 99951999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0177 as found in Promoter Report #51 51tgtgtgtcct aaatagtttc tttttaaaat ttgtaaatac caagacgcgt atttaagagt 60attttgaaaa gatatttgat tataaaaaga aagaaaaaga gaaggctgag gattaactgc 120aacgtctacc gttggaaaag aaaaacgatc agaaaacaca gaaattaata aaaagagaga 180aaaaaaaata gagtatgaga gatgcacatg ggtgcctgca aaaaaaaggt agaagaaatt 240tgtctgaaag tgtcacaggc acactctctc gaaccacatt taacaacact ccaaacactc 300ttcttctact ttgtaccctt cagtacatta ctctttccaa agtccgtgat ttacgctctt 360cgatgacacc tctcaacaga

gagagactac atgtgtacat tttcttctac cattaaattt 420tgaagatttt cgatgattca atttagtata tatatggaag ataaaatttt cattgtcttt 480ctacatgata gtaacggttt tagaagggtg gttatcactt atagtatttg agttaagaaa 540tataaaaata tacgtgactg tttttccttg taaactattt ttaggccctt atttttattc 600aagtagtcac atacgtgttt gaagtgtatt taactaagaa aaagaaagta ggaaatgaaa 660aggatatagt atttatggtg taatcttggt aaggaccagg agatcagaag gggccacaat 720gtcacaaaga ggaccaacaa tgaaattaaa tcctcagctg gcctttaaca ttttggctcc 780caccatctcc ttccacacat atgcacatgt cttcatgtct ctctctctct atacgttacc 840tacacaaata tgtacagaca aatagcccat tacaaaatct ttatttataa atatatactc 900ctcaactccc tcaatatcca cccatctcct tctccataac tctctctctc tctccctaaa 960cacaaccaaa gacttttatc tctcaggaac cccaaaaac 999521006DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0192 as found in Promoter Report #52 52tcctcctact gtctgctacg tcaacaagtg gattgcaatc agacggtgat tgtgtctctt 60ttcattctct ctcttttact aatttctctg ataattaaac tgagaatgta tattaagaaa 120aaaaaacaaa aacaagagag gaattttcat acacactaac ttaagactct ttgtaagttt 180tcccaaatat ggattttcta gtataaatat gagttcatta gtttcaccaa gcctacaagc 240atctctccat ctcaaatcat attcacctaa aaatcaggtc ccctctcttt atatctctaa 300cattcttata tcagatcata ttttttggat ttcttgttaa gtaacaccaa tcttttaaaa 360gtgttttcag gttaatataa aagaataatg atgttttcgg tgacggttgc gatccttgtt 420tgtcttattg gctacattta ccgatcattt aagcctccac caccgcgaat ctgcggccat 480cctaacggtc ctccggttac ttctccgaga atcaagctca gtgatggaag atatcttgct 540tatagagaat ctggggttga tagagacaat gctaactaca agatcattgt cgttcatggc 600ctcaacagct ccaaagacac tgaattttcc atccctaagg ttcactctta ttctcaatat 660taactctcgt acatgtcaca tgcccatttt caccatttta gatatacagt tttgatactt 720tactttgcat ttattttgct atatgtaatt gaggatattg ttttaatttc tttgggtttt 780tttttttcgg ctaaatgaga attcactgtc tttggttctt gaaaaaaaag tatttgttaa 840tggtaaacgc taaacgctat ttgagtttat gttttttcaa gaactgaaaa cgttttattg 900aaaatataca ctttttttgc tatttatagg aaagcatatc acatcacatc tagacgcaaa 960cgcaaaattg agttttaaag caaccacaat cttaaatgca atgaaa 1006531071DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0204 as found in Promoter Report #53 53aactaattag gtcgttaatt gtccaagggt ttttcatagt tgatatagtt ctgttcaaat 60atagccatcc ttaatcgatt catgggatcg taaattacta cttcgagtgt tgtaaaaaaa 120aatgaaactt ctacattaca aactcgaatt taatgcatct ggagtgatac tataaaagta 180gggatgctct caggtcgcat ttgagagaca cagaaatgat tttaatggaa ttaatatatt 240ttcagttttt cacaaaaaaa aattgtgttt ataacaactg cagattcaat gctgatttta 300tgagtctcac ctatagaatt tatatttcta tattcataga ggcagtatag gtgttgaccc 360aacatcgaaa gaacacttcg taaaaaattc tttggaacaa ggctgaaaat ttactcccaa 420atttagctat ccgatgaaga taaatcattt accgtttatt aaagaattat cgagatttta 480gtcaaaacca aaagagatta tgagcctaag attttgaatt tgtattggta aaggaaattg 540aacgaaaatt tcagaaaaaa atattaataa attgaacgat agagttcact tactacatag 600tcaactagtg cctagctata atagtttcaa aagacaaaaa aaacaaaatc ggttaactac 660ttccgtgaca taattctcat cttgattttt gaatccagtc taatttgaaa agtatattca 720aaatctttaa atccattaat gataactttt ataatacgtt gacacacgca attgtatata 780caatattctt gaattttaaa tgtaaattct agaatatatt gcgatcacca cactaatcaa 840aatctttggg acaacttgaa cccacatttg acttttcttg gtcaaatatt ttggcatcat 900gcatgatctt ctctataaaa accaaaaggc ctcaacgaca ttcataaact cagtcattat 960atttattttt gttgtatttc aacgttcaat ctctgaaaac cattgcactg ggatccaaca 1020atgtcctccg actcgtccaa gatcaagagg aagcggaacc gcatcccgct c 1071541191DNAOryza sativamisc_featureCeres Promoter construct pCRS-HT2-pGluB-1 as found in Promoter Report #58 54gatctcgatt tttgaggaat tttagaagtt gaacagagtc aatcgaacag acagttgaag 60agatatggat tttctaagat cgggggaccg agtggaccgg acgaggatgt ggcctaggac 120gagtgcacaa ggctagtgga ctcggtcccc gcgcggtatc ccgagtggtc cactgtctgc 180aaacacgatt cacatagagc gggcagacgc gggagccgtc ctaggtgcac cggaagcaaa 240tccgtcgcct gggtggattt gagtgacacg gcccacgtgt agcctcacag ctctccgtgg 300tcagatgtgt aaaattatca taatatgtgt ttttcaaata gttaaataat atatataggc 360aagttatatg ggtcaataag cagtaaaaag gcttatgaca tggtaaaatt acttacacca 420atatgcctta ctgtctgata tattttacat gacaacaaag ttacaagtac gtcatttaaa 480aatacaagtt acttatcaat tgtagtgtat caagtaaatg acaacaaacc tacaaatttg 540ctattttgaa ggaacactta aaaaaatcaa taggcaagtt atatagtcaa taaactgcaa 600gaaggcttat gacatggaaa aattacatac accaatatgc tttattgtcc ggtatatttt 660acaagacaac aaagttataa gtatgtcatt taaaaataca agttacttat caattgtcaa 720gtaaatgaaa acaaacctac aaatttgtta ttttgaagga acacctaaat tatcaaatat 780agcttgctac gcaaaatgac aacatgctta caagttatta tcatcttaaa gttagactca 840tcttctcaag cataagagct ttatggtgca aaaacaaata taatgacaag gcaaagatac 900atacatatta agagtatgga cagacatttc tttaacaaac tccatttgta ttactccaaa 960agcaccagaa gtttgtcatg gctgagtcat gaaatgtata gttcaatctt gcaaagttgc 1020ctttcctttt gtactgtgtt ttaacactac aagccatata ttgtctgtac gtgcaacaaa 1080ctatatcacc atgtatccca agatgctttt ttattgctat ataaactagc ttggtctgtc 1140tttgaactca catcaattag cttaagtttc cataagcaag tacaaatagc t 1191551000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0008 as found in Promoter Report #60 55ctcgagagat gaagtcttag taatgtttga acaaacaata atcacgtttt ccatcaaatt 60cgagcattta aagtttatat tactacatgc cccaagatga taccgtccat ctcatccgaa 120aatatttctg aaattgcgct aagacaacaa tgtttgctca aattcgatca tttaaagttt 180acaaatctct catcaatctt acaaacttct cacactaaac agaggtacat attttcttat 240aaagacaaaa ggttcgaaca gctggcttct caactcgagt tgtttgtcag ggcctctctt 300cactaactac aagttggtac ttcaaatatt ggtggctagc ttcacgtgat attgtctaca 360aattaaaccc atgaaaaagc tgcattaatt gttccaagtg aaccctgagg agtgtcaata 420gtctttgctt tagtgtgatc attaaaccaa atctctaaat tcctaatttg tactaacatt 480tggaacgtat ttcctactct tctccctgct ccaactccca aaaataagat tagttagatt 540tctataacta atatacatgt atactcccaa aaacagtaaa accatattaa taaagctaat 600tttgcataga tttatttcgg taaaccggcg gttcaagttg gggaaaaaaa agacaaacgg 660tctaaagtca tccaaagaca aaaaaccaaa gacaagttga gagagacgag accaatcaca 720acattgcttc gtagattgcg tgacatcatc cttgacggct actttcattt gtgtcttatt 780tggataaaac gcacgtgttt aattcacgaa ccttcatagc aataagaaat ttccattact 840ttcatatttt caactttttt tattacccat tacatgctta aaatattaat tcacaagtct 900ttgtcaaaat tcaatatttt ccaggttcat gaaccctttt tatctcaatc tactctataa 960tatctcccta taaattacaa caaaacctct ttatttttca 1000561000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0009 as found in Promoter Report #61 56aattcgtttt ttatactccc tctgttccaa gatacttgat attttgggtt tttgcacaag 60aattaagaaa agtaactttt atatttttaa ttattctttt agttagttta ataatattaa 120ttttacttct ctcatttcat tattggttac aaacaaaaat aataatgata gtttttcaaa 180acatcaattt tggtggaaca aataaaaaaa ctcaaaatat caaataactt gaaacagagg 240gagtagttaa ttaaaaaaag atatttcaca ctttgacttg gcgaagcctc ataacaatga 300agttatgtat gaactatata tgaagttaga aacaatggaa aacagcttgt aaatattcat 360tgttgtatat atgttttttt gggtcaattt ggtgcatgaa caaaaataaa aacgtagatg 420aaaaccggat attttggtgt taacatttgc atttgaactt cgtgaaagac ggataaaagc 480tcatttttgt tttttattat atggctgcta ttagtacaca gagttgaact ttagaatact 540aaaaatctcg acatctttta ttttattttt gtcaagcatc gacatctttt ctgttcaaga 600aaacgaccgc aatagtcgaa taatataact cttggactag ttaatatata tttgcgatag 660attttcgatc tcacttatat cttataacca agagacaaaa acaatattgc agtcaagtac 720aaaacgaaaa caatcacaat gtcgactata gatgagtcgg tcattcgatc caacggctct 780gagtccacga aacacgcaac caagtggtgc tctcttttac accaaatcat attatataaa 840acttaaaaga aagagaggat ggttcgttgg ctccttcttg ttccttaatt aattcaaatt 900atattcatca cctccattga ataagtccat ttcacgacaa agtcaccaat gcttctttta 960catgtatata tacttctttc cactccctct tctctactca 1000571000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0010 as found in Promoter Report #62 57aattcgtctt gcattggtaa tccaatattc catgtgatgc ttttctttct aactgaaggt 60attcgtgaaa acgaattcaa gattgtggga gataattagg gtttcgtcaa aagcatgaat 120taggaaaatt tgggattgat tttttgaatt tgggaaaagt cctaatttta attcgatcaa 180agcttaaatg atgtcgtttt gggctgacta acgaagtcgt taacaggtgc caacgagtgt 240taaaaatctg ttatcgcttg ataactcttt ggttttttag tctaatcaac acattctcat 300gtttcaaaca gttaatcaac atattgttca tgttaaaaaa ttaacacatg tgaaattgat 360atataaaaat atcatatatt ttcaaaagtt ggatcattaa ataaaaattt tccctatttt 420tgaaataatt ctaaaacagt attaaagata tttcctgaaa ttgtttgcat gtgatcggtt 480ttggaccgaa actaaaaaac ttagaaacta tattaacttt gagttgtcga gatgaagatg 540tgaaatagaa atataggatc aatggttaga attttggcaa atgtatgaag gtgtgtttga 600ttgaatattc caagtccttt gccttttgga ataggcataa ctactacaac aaagttttga 660taggttttcc gaattcttta aactccttaa atttttaata catctcaatc aaactccact 720tatactaaaa ataatccata ttgcattttt aaaaatccta aaagaagaat cacatgataa 780cttgataagc acttttaaat gatagatgtc cacgtagagg aataagacaa aaagacaaaa 840taaagaaaaa ggacgaaatc taaagagaga aaataagtaa caagtccaag aaaaggtagt 900atgatctttc tcggtccgat cctcgaaact ccctcgaagg ctcgaaccct ctctttgttt 960ttttaccact atataagaaa gtccgattcc tcgtcactct 1000581054DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0039 as found in Promoter Report #64 58ccgttcgagt atttgaaaat ttcgggtaca cccgcctaaa taggcggacc ttatctagta 60tatatataca tttgaactat attgtttact ttttagttga tttaggctat gtcatgacat 120tgacataaat ctacctgtta tttatcacgt gtaattcgtg taaagtgtaa actagaaagt 180tcaaatacgt atttgttttt gttctgttat ataggattgt catagttgta aatctacaat 240ttattacaac atgaataagt acacaagcaa tgtaattgga tttaattgct aaactcttta 300catggtcaat ctaaatttga taagaaatac gtcacatatt actaagactg atagtttttt 360tgttgtcacc aattattttt gttaaattga cgaaaacaat tccaaaaact caaatgtaca 420aaatcataca gtctcacaaa catctcatag agaaagatat aaatctccca tatgggaacg 480ataacacgag gtcgaaatac tattcgtaaa actaaaacgc cttagttata aatcgttagt 540tgtaaccgcg gtcgagaata catacagatc cacgaaacta ctactacaca tgctgctgaa 600ttggaatttg gaaaagacca tcttctttag gaagagctca cccaatgagt gacaaaggtg 660tcggtggctt gttttctacc catatgtata catcaaatgg tagtttcatt aacgtttggt 720tttgagaaaa gtaagacttt ggctagtagc taggttcgta tataataaac tcttttgaga 780aagttcatca ctggtggaaa atgttaaacc ggttttttct cattttttcc gccatgttaa 840ccaccggttt aaaaagaccg taacacattg aaagattaat aagggtatat ttgtaattac 900ggtttgctgg caatttttaa ttattatttt aattagagaa aatagagaag ccctatcaat 960gtacatggta tatatataaa aggcaaaacc ctagaaaacg atactattcg actcagccgt 1020cctttcgcaa gaaaccattt ggagttggag cttt 1054591007DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0070 as found in Promoter Report #66 59ttggcgttaa tcgttaagaa aaaaacgtgc ctacctagcc aaatgacatc ttcctctttt 60gtgtttagca caagtgaatg atccaaatat catctaccta atatgaccca taaaatagta 120tgtgattgtc tttggatgtt atcccataac ataacttaaa tttgggatgc atgcatatat 180tatatacttc atataaaatg agtggacctc ataagttgcg gtttcgattt ttatcaaaaa 240caacagttgt ccactttttg attttgacac acacccacat tcaagcatct cctttcttct 300atattattac tcaaagaata acacccccta aggataacac cattacacaa atcaattctc 360agttaatcat ttgtttcaat atatgttagt acttaagaaa tattcgaata gttttacgta 420ttcaaatttt ataaattcag tgaatgttta attaccaagt cattttggct tggaatctat 480tcaattattc aaaaaaataa ataaataata ctgcatttta acgtatcagc cagtcaatat 540attacacgtg tcataaagca ttacccacac gtttctcctg cctctttccc tttcattttt 600ttttttcatt ttttttgttt cttctcttgt tgttgttatc ttcttctagc tagctctgtg 660aaacacttca aatgaacaaa tatatcaaat aataatagtg taattaagtc ggaggaaaac 720aacaagaacc cagaaagagg aacgaagaaa ctaattcaaa ggtatgatct tttattcttt 780gagagaatta tgttttcttt gtagcaaatc ctctggtctt acgtttagtc actgaatttg 840ttccacttct ggaaaaatct agacaccaaa agcaatctat gaactttaat ttccaatcac 900taccgtcttt tgaggttgta aaatcggtaa atggcagatt tttcactctc attcctttgt 960ggtgatagga tcaagttcac cgcttcattt tagtcattaa acaaaga 100760999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0086 as found in Promoter Report #68 60cttatccttt aacaatgaac aggtttttag aggtagcttg atgattcctg cacatgtgat 60cttggcttca ggcttaattt tccaggtaaa gcattatgag atactcttat atctcttaca 120tacttttgag ataatgcaca agaacttcat aactatatgc tttagtttct gcatttgaca 180ctgccaaatt cattaatctc taatatcttt gttgttgatc tttggtagac atgggtacta 240gaaaaagcaa actacaccaa ggtaaaatac ttttgtacaa acataaactc gttatcacgg 300aacatcaatg gagtgtatat ctaacggagt gtagaaacat ttgattattg caggaagcta 360tctcaggata ttatcggttt atatggaatc tcttctacgc agagtatctg ttattcccct 420tcctctagct ttcaatttca tggtgaggat atgcagtttt ctttgtatat cattcttctt 480cttctttgta gcttggagtc aaaatcggtt ccttcatgta catacatcaa ggatatgtcc 540ttctgaattt ttatatcttg caataaaaat gcttgtacca attgaaacac cagctttttg 600agttctatga tcactgactt ggttctaacc aaaaaaaaaa aaatgtttaa tttacatatc 660taaaagtagg tttagggaaa cctaaacagt aaaatatttg tatattattc gaatttcact 720catcataaaa acttaaattg caccataaaa ttttgtttta ctattaatga tgtaatttgt 780gtaacttaag ataaaaataa tattccgtaa gttaaccggc taaaaccacg tataaaccag 840ggaacctgtt aaaccggttc tttactggat aaagaaatga aagcccatgt agacagctcc 900attagagccc aaaccctaaa tttctcatct atataaaagg agtgacatta gggtttttgt 960tcgtcctctt aaagcttctc gttttctctg ccgtctctc 999611106DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0088 as found in Promoter Report #69 61tcgattggga ttactacttc atctagtaag gttctgaaaa cgtttgttgt tgataaggaa 60gattcgtctc aggttattac tgttgatctt caaggtttgt gattgtgacg cttatacatg 120tgctgaaact gtggtgttta tttattgaaa acaaaaaaaa agtctctctt gtagtttcat 180tgtactaaat agaaaacaag aaacgttttt ttctttaatc ttctacattg ataatattgg 240atcaaaggat tgtttctgca agacacaaca caaacatact tatactagtt tacttctact 300aagtactaac tacataccca tacacacact tgcacctaga ctttacttct agacatcatt 360accctaaggt agaaccaagc ttacaagcaa gttttaccga caactcttac attacaactc 420tagtctgtag tctttaacgt agacttacta actagtcatt agtggtttaa ttttttaaat 480tttcatccat atgtttttgt tgtagatata aactaaagtc ggtcacattt aataattgtc 540attatgtccg cgtaaaagtc aattcagcta ttggacattt atgaaatgta agattttctc 600tctcatttcc ccgtgcgtga agacatgcat tggtttttct gtaataatca acaaatccaa 660accccttttc gatctttatt tggacattgt tagagacaaa atttctctat agtctttttc 720ctaatttgat accatgtttt tgtttctgca caaatttact cactggttta actaactatc 780cacttattta tgattttacc attaggcgtc agctagccct agtcaaattt gtaaacaagc 840caagctatct acataaatcg agatgtcatt aacgttaatc gtcgttaatt cgaatttgaa 900aacatagata gctttagcag tacaatgggc aatggtaaga agaatagcaa aaggcccaat 960atttggtttg cagaaattaa agccttaaaa aaaagcccac agatatttgt caaagaaccc 1020taataacctt ttcggtgctc aagcgccgtt tctagggttt ttctctgagg aagaaagcgt 1080ttcatttctc tgaatttcat cgaaaa 1106621004DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0101 as found in Promoter Report #70 62ttctcgttct ctagaatatt gctggaccgg attaggtcaa tattattggg ccagattaga 60tattgaattg tcgacgttgc ttacgttacg ttatatcttg tttaagaatt aaacctatcg 120acttagtctt aattaagaaa acattgcctt aaattctctg gtctgcgacc gtttttttga 180ccgttaaccc ctaattaaag aaacaaaata attatagaaa gagcactgaa atgtgattat 240tttaacagta ctcttatgag aaaattcgta ctttttagtt ttttttttgt acaaatctct 300aagaaaaaca ctactactaa ttaagaaacg tttcaaacaa ttttattttc gttggctcat 360aatctttctt tctcggtccg ggactaaccg ttggcaaaaa aaaaaaaaaa gttgacaata 420attattaaag cgtaaatcat acctctcaaa taaaaacttg aatttggaaa caaagacaac 480taaaaaactc gaatttaaga gaattcctaa aatcaagtga agtatcatca cttggtaaaa 540tttcataacc gttggcttct atttctatgt gtgccttggt ttgcaggaga taatatttca 600tttccaacca atgatattcg tacacatagt caaacaaatg tttgtctttg ttattatatt 660gagaaagaaa caagaaagag agagagagat agataagacg aaggaagtga agcttccaag 720cgcccaccgt taaaaatctc gtgtgcaagt ttcaaataca agtggccggt ggtctccata 780atttgatcgt catccaatta aaaaggaaga aaaagcgtgt tttatacaag aaaactcatt 840aaaatagcaa gtctagaaat atctcaacac taatctacca cgtctattac acacacacac 900acacacactt gatcttaatt tattttcaag attcaagaaa atacccattc cattaccaca 960acttgaccac acgcctatat ataaaacata aaagcccttt cccc 1004631000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0115 as found in Promoter Report #71 63atgatgaaca ttctacatat ataattatta tgtttaagca cttagacagc ataaattctt 60tctaattata taaatctaac cttgttacat tgtacatcta taaattactt gaagaaataa 120cgagttctat ttctttttaa aaattaaaaa tactatacca tatctcagtg attaagttga 180accaaaaggt acggaggaga aacaagcatt tgattcttcc ttattttatt ttattcatct 240ctcactaatg atggtggaga aaaaaagaaa atacctaaca aacaaatata tattgtcata 300caaaaatatt tctatatttt tagttaatta gtttatattc ctcacttttc agggcttata 360taagaaagtg agcaaacaca aatcaaaatg cagcagcaaa tactatcatc acccatctcc 420ttagttctat tttataattc ctcttctttt tgttcatagc tttgtaatta tagtcttatt 480tctctttaag gctcaataag aggaggtact attactacac ttctctctac ttttacttgt 540attttagcat taaaatccta aaatccgttt taaattcaaa aataaactta gagatgttta 600atctcgattc ggtttttcgg ctttaggaga ataattatat gaaattagta tggatatctt 660tactagtttc cattcaaatg attctgattt caatctaata ctctcactct ttaattaaac 720tatatgtagt gtaatttcac actgttaaat ttctaccatg tcatgtatat tagagttgca 780tagaaaattg taaaacatcc atttgaattc gaatgaaaca aaatgtttta aaataaaatt 840ttggttttta aaagaaaaat ctaaaactga attatatcgt ttaaccaagt tgtaaaagtc 900ataaaacgta gtatcttgta aatcgctctt ccacggtcca aatagacttc tagtaataaa 960caagtaaaac taattttggt ttcttactaa ttttcacaga 1000641009DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0133 as found in Promoter Report #72 64gtcgattggt attagtccaa taactttcac agttttacta taatataata ttgaaagtat 60ggttaattga caatagggta tttccactga taatcagtca ccagtaataa taatttgttg 120ggactagaat caatcacttg agttttaaat tgatatatgc ctagatggtt gttaggacca 180atattcctaa acaataccaa attttaatat agaaatgtaa aataattcgc cgacacatta 240tttcgttatt gacattgaat atttcaatga tatcaactct gtttatttac atctatttag 300tataatttct tgagcaacaa aaaaagtagt tttaaagtta ttttccctag atttttctat 360tgtaaaagcc tatatatcac aacaaaaatt aaccattttc tcttttggca agtttaaaat

420ttttataatg aatataccat tttaaaaaaa aaattatcca aactaaatta accattttct 480cttttggcaa caaaaattaa ccatttaaaa aaaaaaaaaa aaaaaaagtt taaaattttt 540ataacaacag aaagtaatat tatccaaact atgaataatt aaaaaatctg tccacatacg 600atcatatatt tctctccacc gacagccgaa aacactgtca atggcccacg ttcctctaaa 660agctgtcgtc ttcggtaata ttttccggta ataaactaac ttccgatcac aattacacaa 720aagccccttt ctcgtttata atcataggct atgattcata gcaaacttac agagttggtt 780attaagaagt caaaaataca gattccttaa aatatttttt ttctgttcat atatttttta 840tcgagaaaca attacttaaa cataagaagc aagacaagaa ttaatgttct tattaacatg 900atgctaatat aatcggaaaa caaaatcaaa tcatgataga agggtaaaat ggtcaaatca 960tcgttcccgg ctcaaatttt actaaaacag ccactagcca gccagatcc 1009651001DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0137 as found in Promoter Report #73 65gtggcacatg ctgaaacccc gagcatctct ccggaagaca cgcgtcgttc gctccaaaga 60aaacagtcac agctgccgga gaatctccgc cgtcttcttc tgccaccgga aaaactctct 120ccaccacttt cagtgcccac ctcgtgttat atccactgta tcctcgtagc accatatcag 180cctaataaaa ttttatgtat caaattttaa gacatagccg aaactacact atactagaca 240ataataatat gatttgtttc ctgaaaaatt atggtttcat gagaaacatt aatcatctat 300aaaacaaatt agctatggca tcgaagagtt atcaatcaaa actgatgaat ctttacttaa 360tatatacaac atatctttac cttgcggcgg agaagatcgg cgagagaagc accccagcca 420ccgtcactaa aggattcttc agtgatggaa tcaccaaaga gaaaaacctt ccgtctcatc 480atcttccaca caatcttctt gagaaaatct gagagataag aaaggtgtag tggttttgct 540gaagtgatcg tgtttgattt agtaaagaaa tgctttattt attgttgggg gaaacataaa 600taaataaagt aaaagtggat gcactaaatg ctttcaccca ctaatcaccg acctttcatg 660gtttattgtg aaatacactc atagatagac atacaatacc ttatgtacgt aaataacatt 720ttatttgtcg acacttatgt aagtaacgca tagattattt tctatgtgat tgccactctc 780agactctcag tttcaaccaa taataacaat aactacaaca acattaatca taaacatatg 840ctctggttta caattaaagc ttagattaag aaactgtaac aacgttacag aaaaaaaatg 900ttatttacgt tttgtaagat tagtctctag aatcatcacc gttttttata tattaatgat 960tctttcttat atataaaacc tttctcgaaa tacccatgaa a 1001661003DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0144 as found in Promoter Report #74 66aaacgttgca agattattga ttgtgagaaa gagtgctcaa ggtagtactg atttctgtaa 60agctcacggt ggtgggaaac gatgttcttg gggagatggg aaatgtgaga aaatttgcta 120gaggaaagaa gcggtttatg cgctgcgcat aacactatta tgtctcggga gaacaaagat 180ggaagcaaga gcggtttgat tggaccggga ctctttagtg gccttgtttt tggctctact 240tctgatcatt ctcagtctgg agctagcgct gtctctgatt gtactgattc tgttgaacga 300atacagtttg agaataggca gaagaacaag aagatgatga taccgatgca ggttctagta 360ccttcatcaa tgaaatctcc aagtaattca catgaaggag aaacaaacat ctatgacttc 420atggttccgg aggagagagt tcacggcggt gggctagtaa tgtctttact tggtggctcc 480attgatcgaa actgaaagcc atttatggta aaagtgtcac attctcagca aaaacctgtg 540taaagctgta aaatgtgtgg gaatctccga atctgtttgt agccggttac gttatgctgg 600atcaaaaact caagatttgt tggatattgt tatgctggat cggtggtgaa accacttccc 660ggttgctaaa taaataaacg tttttgtttt ataatctttt tcactaaacg gcagtatggg 720cctttagtgg gcttccttta agcgaccaat acaatcgtcg caccggaatc tactaccatt 780tataggttta ttcatgtaaa acctcggaaa atttgagagc cacaacggtc aagagacaaa 840aacaacttga agataaaggg ataaggaagg cttcctacat gatggacaac atttctttcc 900acacaaattc tcataataaa aatcttataa tacaaatact tacgtcataa tcattcaatc 960tagtccccat gttttaaggt cctgtttctt gtctgataca aat 1003671001DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0143 as found in Promoter Report #75 67atacaacaga tggcagatat cgagttaaat acgtgaatca gccgttacga tattttaaaa 60ctagaaaatt atttaaaaat attgcaaaat accatttaat ttcattgttc ataaaaaaaa 120gaaattcaaa aacttaaaaa ctgattcaaa aatttggatt aattctcatt aacagtcttc 180aacactacaa caacatgttt ctaatttatt ttatatttta ataattaaac aatatatacg 240tctgcacatt gttgctccga cataatctag tataaaaata gttgcagcat atgtgaaaag 300caagcagcat ttatcactca atacttttaa ttttatctgt tgtatgtatt aaggttttgt 360agctttaaga aaacgcttat aatataaaat aacttctaaa agatatttca tgcgtataca 420ataaatattt gtgaaaaaac atttcgaaaa cgtgtacaat atataaacta ttgtgttatc 480ttttgacatt caaacaaatg ttgacaatgt aattttatcc atgatatgat tggccaatta 540gctgcgaggt aaaaatccgt atacgagtaa aagtaagata aaatttcgca agaagatttt 600tagcaggaaa tctaagacaa gtgtcatgaa cgtgtcaatc aacaaacgaa aaggagaatt 660atagaatcca gattcgacgt accacattaa taaatatcaa aacattttat gttattttat 720ttttgctctg gcagttacac tctttttcat tgctccaata aaaaaatcac tcgcatgcat 780gcatatatat acaccatagt aaactccgcc tcttcttcat tttaaaagta tcagtttaca 840ctgacacaat ccttaactat tttcctttgt tcttcttcat ctttattaca catttttttc 900aaggtaacaa ataatctttt taagtcactt ttatactctt taaatcttag attgatatat 960gaatgcatgt taatatttca agatttatag gtctaccaaa c 1001681004DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0156 as found in Promoter Report #76 68ttggtttgca ttgtgaagat ttgtattaac tatagaacat tgaattgatg gtgttaagtt 60cttacacaag cgtgcttctc ggtttgaact gtttcttttg tatgttgaat cagagcttag 120tttataggaa ccagagtatc tacttagtca ttctctgatg ctaagtgcta aggttctacc 180tagttgccct ctaggccctt atgttattga taacttatga agctatttga acacttgatt 240cttaggagac ctaagttggt acagccagat agagtgtatg ttcttgttct ctatgtgaca 300ggatcaagct gccacacata gttcaagggt atgctctgtg tgggtttgct cagattgagg 360acaaatctat acaaggaagt agagtctttg acattttgat gttgtatgat aagaagaaga 420aaggagagta ataaagaaag agaaaaggga aacagaaaca cgtgggagaa catcccaaag 480aggaagcaca cgcggatctt catgcaaagc tccccgattc tcccatgtgg tccctttctc 540cctttgtccc cctcctcttt cttcttttct cattttactc ctttttttac cattatacaa 600cgaatctttt ttatcataat tttttggttt tggtttattt tccaataaca ctttcttggt 660tacttcccat tctcactttt tcatataaga aactcacttt gggaaactta tgtttgagaa 720tgacaagtct ttttagagaa agtgatgtaa caaatctaaa gtgattatat aataaccttg 780cacaatgttt ttgatttttt gtaagattcg aatattaggt ttattattcg tagggaataa 840acttactttc aaaagcgttc ataagttaat actttcatat atgatcataa gtacggacac 900tattgttttt tgtttgtttg tgtttattct aaaagaaagt agcttttaat tgaaatgtcc 960tcggaggcac agtttaaagt tcgagtgtaa cagtttctaa ggca 1004691000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0158 as found in Promoter Report #77 69ttattagatt aatagattgc attgcattgc ttgtgctttc aatttacaaa ttgtctccca 60actccatcga cacatctctt tttgtgtata taagattcag acttgttata ttttttttat 120aaatatgtta ttagcatctt aagttaaatt gattttttat atctgcatta aggattacac 180gactatattt gcgattgtgt gttggttaaa atataattta ggattgtctt taactacatt 240taggattata tgactatatt tggttaaata taaaatctag ctgtgattat tagtattcaa 300aaataagtag cctaaccaat taaaacaacg gctattgggg caaattagaa cattttagtg 360tgtccaaaat ataatggtca ttaggtcata ttcctcctag cttcatcgca gcataattga 420atgattgcct tatttagaag agcttttcca ctttcccaaa atctaggtgg gatctttttg 480ttttgacctt catttttctt gtttaccatt tttagctaaa ttatttacga ttacaaaaga 540tatcaaaagt tggatcataa tacaatttat agacttactg tagaaaattc gtatgtacaa 600gtacaacaaa ttcttcataa taaattttga aaattctatt acaaatgttg taagaaatag 660aatttgaaat atatataaac taaggagaaa aaaaaagaga acatgcattg ctctagtcag 720agtggaccaa catcaacgag ataagataac ataaaaacca actcaccata actaaaaaca 780tcccaagaga tccaacgatt catatcaaac acaaaaacat cgaacgatca gatttaaacc 840atctctggta tctccaaaac acaaacactt ttttttttct tttgtctgaa tggaacaaaa 900gcatgcgaca tctctgtgtc tttatcttct ctctcctctt cttgaaaaac tgaaccttta 960attctttctt cacatctcct ttagctttct gaagctgcta 100070997DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0164 as found in Promoter Report #79 70gtcgattggc attactagag ttttagattt cacagaattc ttttctttgt ttggtctgtt 60cgtcctctac tgtgtttttc ttttgttttg gctattttgc cgtctgttaa aaaattgggg 120ttttccgata atgtattgta tatgcctgaa ttatggaaat atcactaaaa acactttttt 180gcacttttgt atatattgca attaagattt aatttttcta ttttgaagac tttttgttaa 240aagagagatt tgctaactca aaattaccgg agttaataag ttcgttctca tcagattagc 300aaaacattca tctttaaaga aatctgttta tctcacgtaa aattctacta attggcagca 360gcacactact ttaatgggcc tattactatt tcaatatgca ctgaaaactt cattggactg 420tccaataaca ttataacatt attaaaacta aactacattt gtatttgggt tacaatttta 480taaagagtaa gactcaagat actaattggg ctgcagacaa ctttaaaagc cttactacaa 540aaatcaaaac cattcttaat taactaacaa ccaggtaaaa atctcagtac aatatttaca 600ctaaaaatag gtgccaccca ctatgcaaaa gtttagggcc acgaacaaaa aaacctgtaa 660attgttatgt cttcacaata tgtgttttaa tacacatgaa ttttagctgc ggttaatgta 720aattttgtag ttaaattaag gctaaacaat ctcaaatata aattggtcag gtccacagac 780aacagcctgg caagtggcaa gcactaaaaa ttcgaccgtt attttttgcc ccttttttta 840atatttcgaa attgtatctt ttagttttat tttaaagctt tttagcccgc tcctcctccg 900ctccaccttt aattttttca ccaattggat ttggatctgt caaaaatatt ggcctctttc 960tctctttctc tcttgctctc tttctttgtt gggttga 997711000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0119 as found in Promoter Report #80 71taccaaaaat aaggagtttc caaaagatgg ttctgatgag aaacagagcc catccctctc 60cttttcccct tcccatgaaa gaaatcggat ggtcctcctt caatgtcctc cacctactct 120tctcttcttt ctttttttct ttcttattat taaccattta attaatttcc ccttcaattt 180cagtttctag ttctgtaaaa agaaaataca catctcactt atagatatcc atatctattt 240atatgcatgt atagagaata aaaaagtgtg agtttctagg tatgttgagt atgtgctgtt 300tggacaattg ttagatgatc tgtccatttt tttctttttt cttctgtgta taaatatatt 360tgagcacaaa gaaaaactaa taaccttctg ttttcagcaa gtagggtctt ataaccttca 420aagaaatatt ccttcaattg aaaacccata aaccaaaata gatattacaa aaggaaagag 480agatattttc aagaacaaca taattagaaa agcagaagca gcagttaagt ggtactgaga 540taaatgatat agtttctctt caagaacagt ttctcattac ccaccttctc ctttttgctg 600atctatcgta atcttgagaa ctcaggtaag gttgtgaata ttatgcacca ttcattaacc 660ctaaaaataa gagatttaaa ataaatgttt cttctttctc tgattcttgt gtaaccaatt 720catgggtttg atatgtttct tggttattgc ttatcaacaa agagatttga tcattataaa 780gtagattaat aactcttaaa cacacaaagt ttctttattt tttagttaca tccctaattc 840tagaccagaa catggatttg atctatttct tggttatgta ttcttgatca ggaaaaggga 900tttgatcatc aagattagcc ttctctctct ctctctagat atctttcttg aatttagaaa 960tctttattta attatttggt gatgtcatat ataggatcaa 1000721000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0171 as found in Promoter Report #82 72aatgaaaaca atgggagaag tacatgcttg tcctcacaca ctaatgccga tgaggaaagc 60ctttaagggg acttttatct ccgcaggagg tttcacgagg gaagatggga atgaggctgt 120gtcaaaggga agaactgatt tggtggctta tggtcgatgg tttctagcca acccggacct 180gccaaagagg ttccaagtgg atgcaccgct gaataagtac gatagaccaa cgttttacac 240ttctgatcca gtcgtcggtt acaccgatta ccctttcctc gaatcaacag cttaaaattg 300ttatcaataa tgtaatgtag tgtgtttccc ttatataaga tgtaataagt ttctggcttt 360tcatttatac tttttaagtt taagtcataa aaccttcaca aaaatttcca cggacacatt 420atcacaaaag cgctttctag agaccaacat aacttaactt gattgttgat ttctgtttga 480tgtgatcatg ccgcaatcca gtgtgttctc atgatgctat cttccctcct ttcacatgct 540gcacagaaca aaacagagca ttttcctccc aactatacct aatttttttt ggtcggtggt 600caaagttata catcggaaga atctgttgaa atcatattga ggcccctcat ttgttatgtt 660atgtaatctc caatggatca aaagtagaat cccaactgta gaagatgata ctatcatgct 720aggtagaaga cagcatggaa tatggggtaa gttcaaagtg gttacactct aatgtcgtct 780caacgaatac gtctttatga agaaataaaa aaatctaagt ggttggatgc gtcatcaatg 840acgaccacgt cgttaaagac agaaagaaac acttgcgttc ctgattctct gatcaatcaa 900tgtgtataaa tatgttcgga tatgtccatt atcttacgca atcttgaaaa gtgtttttga 960gagaaatata ggttttacaa aatccaccgt tgtgaattca 1000731005DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0188 as found in Promoter Report #84 73gattggtatg aaatttcgga gaccaacaaa aaaaacttta ttgagcttgg agtgaagcta 60tatatatggg gcaagatcat aatatgttta tatcggcctt ttcgttaact gaaaataata 120gttttgagaa atatatcaaa tggtaaacag acatcatctt tgaaaaatac catcaatgaa 180gttaatattg ttattggcat atggtttacc catcttaatt ttaatgcaac caaacaaaca 240agaaacaaaa actgtataag atacaaggtg ttttacgatt ttccgtctta aaaccgaaat 300atttttgttc ctacgacttt aaacggactt tgcttaagtt gtgtgcatgt aagctcgtcg 360tccctcgatt gtcatcaaca ttcaccaata tcagcctcta tcacacgagt gaaggtggtg 420attcggctta atgaaaacag agaaatattt caatatgatt cctattaaat tttaaatctt 480ttttctcaat ctctagattt tcattaaaag catcatgatt tttttccact atgttcatat 540atctctatca cagttttagg tacattgtag aaattggata agatacgtca tacgtctaac 600atgaatttgg tctagcaagg aaggtttgag ataataagtg aaaagaaaac acaagataat 660aaattataat ttataaatgc tttatagtat tgaaaaataa gatgattttt ttttttttta 720ataccggatt ggctgatcca cttatgatga ctcaaatgtt attaagtttc aagacaattt 780atgatgacac aaatcacaat gagtcaatag tagccacgaa gccagaaaaa aaaaatgtac 840tacaaaaaga taatgatagt acaaaatgat acgtcgtact gccacatgta cgacacaact 900cgattaccaa aaagcagagc catccaacca taaaactcaa aacacacaga ttccactggc 960gtgtgctctc ctcacttcac tcgtccttga aacttgaggt actga 1005741000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0258 as found in Promoter Report #85 74taaataactt acttaaattt atattaaaac cgtttacatc tgatataatt taaaaggttt 60tagacgaatt agtattagtg aaccattatc aagatgtatt tattacaaaa tctataataa 120taaaaacaaa actgggaaag caatgggaag aagatctgat tggctttgga agacttcgcg 180tgttaataag atttttctca aaaaagaaag atcggacggc aatgcgtggc aagtcgctta 240tgacgtcgga agcttagctt gccacgtgta cggtcatcac catttaggtg accgatataa 300tgtatttccc gtgacttgac aatctaatct cccaatttat cgtgctccag tgtctcaaga 360gagaacttac tctattggtt acccaaaact cacataaaac gtggatttta tatttttttt 420tatcaaataa tttgatgtgc aatataaaca tatatgtatt attatcttgc atgtttaaaa 480aatgctaact taaatttttc ttcaatgatt gagatttttc atacacatcc atcgagttca 540cacttatgat taaacaaagt gtattgttac actaataacg gttctaagtt gtttcaatac 600gtttatgata acggttctat actaatacta gtattcatac gtttatgaaa ctcgttaatt 660atatatttaa ctttgtttag ctgtatcata acaagcgttt taagaaataa tttaataaaa 720aatgagaaaa cgataagcga cgccttatcg cctatgtgta attatcgtgt caaaatatcg 780tgataaggaa gctgtttcag aggagccttc tcgtttgttg cgtcgttgct ctgagccaac 840aacgctaata taaaaggaag ctcaagtctc tctgttttaa tctcggacca atatacaaaa 900ccgtgtgttc ttctctgtat cttattaaat caaaaccaat tttgttcttc ttctttgatt 960cttttttcct tcatttttta acgtatcttg agagatcgac 1000751000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0265 as found in Promoter Report #86 75gtcgattggt ttttttttta cggtttcctg caaattaatc cattttaaac aaatcctcca 60ttgttttgta taatgttgga gattagtagg attagtcgga taatatggca taaacataat 120atgatgctaa attctaaaga aacgatatga ataaataatg cagcagtata aaaactgtac 180cattgcgtgg gtggaggatt ttaggtcatt gttagggcac tttcatgggg accatttccg 240ccttaacgaa gacttattac acacaaccgt caatgtgggc tggaaacttg cagtgcaatc 300cggtctggtc ctataattta tggccggtta gggtttacaa gtttttaatc tatcaattaa 360atgagatgga cacaaaatgt aatcaccagt ggaaaagaaa acattacgag acttatacaa 420tgtctacaag aagactcgag tggggcaaca tgttgtacat aatccgacgt cgttttggga 480gtcagtaaat aaagttcgag aaatgacgtc gttttgaagt catccgagag aaaatgactt 540aaagtgaccg acgttgtttt gaagtcagcg gaagagtaaa gaagtaagaa acgaagtcgt 600tttgaagtca tctcttcaga tatgtttgtt ctaattaaaa tttcccaagt gggaattagt 660ttgtaattga aggtatgcac gatttttagt tacaatttta attcttcttc ttcagatcca 720agaactctca gtctctgcgt tcacactctt tctttgaatc cttcatcatc ctaattcatc 780tccaagaact gaatcagaag ttgtatttcg ctaattcaac ttttccaggt aattttccat 840tcctcgatta ttcgaatttg ggtattaaat cataatgcat cgggaaattt ggattcttta 900ggataatttt tccggcaaat ccgattattg agctagtttc tgaatgttta gattctcagt 960gtctgtcaat taggttttga ttttggaagt agagaagtta 100076986DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0071 as found in Promoter Report #99 76ataggcccta cttctaatta aagcccattt acttctctcc ttgtcttctt attcctcttt 60tctccccatc acgtgacgac gatgctataa acgccgtcgg attatataac tggtgccgtt 120gacaagacgg cgacagaaga aagaaagaag aaaccacagg ctctagggaa cgtaacgtta 180tgtcctgtct atagcattta taacggtcag atcaacgccg tttagataaa gatctgtcaa 240tgttaaagaa gagatgcatc tctacaccgt taaatttaaa acgccgtgaa cctcttatct 300attgattttt gtttgatgaa gccaaaacaa atcgtgtcag aagacttatc agagaagaag 360aaaacgacga cgttcccgtt tctccatgtc taataagtgt agtagtggcg gctactaaaa 420actctaaagt ttgactccag taaaactgcc tttctagtgt aattccagtg attttagagt 480ttgaatagtg tgtgaccaaa tttgaaagta caatctcagc aatattattg atcactcgtt 540ataaaagaat cgaatgtaaa aatagccaat gagagactga gacgtatgtg tttgaccata 600agtcgtatag tttgtatcta tctacctgca agatcagcag atggttctct gatcaattgt 660accttaatta tcttttattt tcgtaaaatt tctctattca caaatgataa atctacttaa 720gacagtaacc ataacaagat ttacaagata atttgaaaaa tgaacacata aaagtatttt 780ggcgcattat ttttaataat aacaatattt atgtaaagtc acataaaagt atatattcgc 840tcacaaagtc ttacggtatt tagaacagta gtaccacatc gattctcttc atcttcttct 900tcataatatg ccattgttca tgtctctgtg tcctatcgca taacactcac gctatcttat 960tattttctct cgctctttct cactga 986771100DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0214 as found in Promoter Report #100 77ccagtcgatt ggcgcctcgc atgcctatca tatttaaccg tcaataatgg atttggcggt 60tttggtaggc cgggtcaacc ggattaaaag aaaacggttt ggagtccttc cttgcaattg 120aattttcaca cattcgggtt ttgtgatttc tctgtcataa tgggcccggc acatatggtt 180cataacccat gtgggcctat ggtataattt ttccaattaa aactattgtt aggtcgataa 240aacaaaaaac aataaaaacg agtggaatac acataccaaa aagaatgtga tgaacattag 300taattttatt ttgatggtta atgaaaaaca aaataaatgc atcttggcat cttccgttgg 360aaagcgcaaa tagggcagat tttcagacag atatcactat gatggggggt gagagaaaga 420aaacgaggcg tacctaatgt aacactactt aattagtcgt tagttatagg actttttttt 480tgtttgggcc tagttatagg atcataaggt aaaaatgaag aatgaatatt agattagtag 540gagctaatga tggagttaag tatgcacgtg taagaactgg gaagtgaaac ctcctgtatg 600gtgaagaaac tatacaacaa agccctttgt tggtgtatac gtattaattt ttattctttt 660atcacaagcg atacgtatct taagacataa taaatatata tcttactcat aataaatatc 720ttaagatata tatacagtat acacctgtat atatataata aataggcata tagtagaaat 780taatatgagt tgttgttgtt gcaaatatat aaatcaatca aaagatttaa aacccaccat 840tcaatcttgg taagtaacga aaaaaaaggg aagcaagaag

aaccacagaa aagggggcta 900acaactagac acgtagatct tcatctgccc gtccatctaa cctaccacac tctcatcttc 960tttttcccgt gtcagtttgt tatataagct ctcactctcc ggtatatttc cccattgcac 1020tggacactct catcttcttt ttcccgtgtc agtttgttat ataagctctc actctccggt 1080atatttcccc attgcactgg 110078990DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0216 as found in Promoter Report #101 78ttttgtttct aatagtttga tgtttatatc aacattatta tttactttca tttgttaccg 60atagaaagag gagaaaattg ttgacaaaaa caaagataaa agtaaaatta atattattaa 120attaataaaa ataacaaact gtaaaagcta tttttttaaa aattttgtgt aaaacatcta 180aaaattattc ttttagaaac agaagaatat cattgaagat aatagtgtga aattatatat 240atatatagaa atatataaag taggattttt ttctgtatac aaatatacgt ttccaatttt 300atcaaaaact gtaaagattt ttttctttgt cagtacctgc taaacttgtt aattttttta 360ttaaaaaaaa atcaaattac aattcttcta taatcatttt aaattccatt tctttatacc 420acaaaagatt atattgcctt tatcgtcttt ggnatgtatg cgtgaatata tttatttatt 480ttcttttctt tcattttctt tttaaagaac tttataaatg aaataaggaa caaacaatat 540acatatgtac taacgtatat aaataatatc atcaatatct atccaaaact tggatttcat 600ggttgacgtg gcccaaccaa aatctcaagt tctctgcgga tgacgaacca tctcaccatc 660tctttttttc tctctctttt tttttttaat atcatcagca cggttacata aaattcgtga 720tccatgaagt tggctttctt gtcgttttac ttcatcaccc cattttttaa aagtctccat 780ctttatactt cttcaactct ccaccaccac cattgtcacc accacattta aacacacact 840ttcacttgta gtgggattag aaagtgcgtt ttattcattt gttttactgt ttttgataac 900ctcaaaattt gcctaaattt tattctctat aaatccttat atgttttact tacattccta 960aagttttcaa ctttcctgag cttcaaaaag 99079984DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0271 as found in Promoter Report #102 79atctctgatt ttttttatca ggaacaagta aataaatagc tttgagtttt tgtttttttt 60ctacattctt cgcccaaaag atgtaagaaa ataaaggatt tgaaaccttg ttctgttgtt 120actcctttaa attcttagaa actataaatc attatatctt tgatctgttt cacaaactaa 180tcatattcgt tgcaaagtga gaattcgtcc cactttactc tttacaccga tactagtatt 240atagatgtac agcatagtat tccatatcta gttatttagt caaaactcta tatattaaga 300ggtaggttaa ttaattaagg agtaattgaa gattatagaa agaataaaaa ataccattta 360atggacagaa ccaaagataa ctaactatca tactataatg ttgaatttct tccacgatcc 420aatgcatgga taacaacatc aatcaaatca tacattcatg ctatataaca tagttttcag 480ttacaaactc tcttttttat ttatttcagt tgttcctttt catgaccata ttaacatcaa 540ataatgcatt tttttcaacg tctcttgact tacacccact aatattgaca aattgaacat 600ctatacgact atacgcacat aagttaaaaa tgcatgcaag tgctaaggga atttataaca 660tctaaggtta ataagactaa gaaagtataa aataagaata cgtattatga atttatgata 720tactttacta atctttttga aaaatacttt aatttaatct actatagggg gtaaaaagta 780aaaaagaaat aaagatacgt ttatccgcat atagtacctg gaaataacag aaaataaaaa 840cacaggtaag tactttgcct gagctagtat atgaacacta aagagataca cacacacaaa 900aagagagcag aaacaaaaca cacacactta aagctttcgt ctttacctct tcccttctct 960ctctctatct aaaaagagtt ccga 98480979DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0279 as found in Promoter Report #103 80cgctttatta taggtttaac aattgatttt tcattatttt gttttcaatc tccaaatcat 60ttctcaataa ctctcaaaca ttgtttaaag ctttttttct taattaacat tataacaaaa 120aaataaatag agaaatttac tttgattcaa acaccagtca ttgtagatta gccaagagtt 180ttcagtaaca aaatttacct tataaacctt ttgaatggct atttctgaaa tggaatagaa 240atctttagtc gtggaagtat ctctatccat aagaaaactc gttttacaaa gtaattttaa 300atcaatacaa aaaagtgaaa aaatccactg gtggacccca ttcattccag aattgccgat 360tacgagctat cttgtccctt cttcaccatt cgctcactct ctctctctct ctctcgtctt 420cttcttccca ccactctctc tgtttctcca caacttctct tctcaaagtt aaaattaccc 480ctaaaccaaa aaaaaaaaaa cgctcttcac tatttattta ctaaactctc ctttgtttgt 540tactaagctc tcactaaaac cctaatcttt ctcctcttat atatctcgtg actcttcttt 600ctcctccaat ctctctctcc ctcttcacaa accaattagc ttctttctgt aaaacctcac 660tcgttggcca attcttttgg ttttcataca cataaatctc agattccaaa tgggttttct 720tagctctttc tttcaaatga tgaacatttg ttagcagaat cttcctcatt ccctaaagtt 780ttgatctttt tttccccctt caattttgta ttttctcacc aaataaaaaa aggtttcttc 840agtgggtttt aagggtttat tattatctta aaattaaaca caattcttta atcaaaaggc 900aaaaatctta atttcatcac tctcttctca ctcacaaaag ttcttacaat cttcaaagtt 960ttggtcttgt ttcttttcc 97981981DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0285 as found in Promoter Report #105 81gggattatat atgatagacg attgtatttg cgggacattg agatgtttcc gaaaatagtc 60atcaaatatc aaaccagaat ttgatgtgaa aacactaatt aaaacatata attgacaact 120agactatatc atttgttaag ttgagcgttg aaagaaaatg aaagagtgta gactgtagta 180cgtatgagtt tcccaaaaga tggtgcttga atattattgg gaagagactt tggttggttc 240ggttgaatga agatttttac ctgccatgtt gatagagaaa ggcaaataaa tgtaggggtc 300gatgtctaac gtaaagactg gatcaaccaa gagtcctcct cctcgtcttc accaaaaaaa 360aagagtcctc ctcgtggaaa cttatttctt ctccagccaa gatctcatct catctcttca 420ctctatgaaa tataaaggaa tcttatggtt tttctaaaaa ctatagtacg tctatatacc 480aaaggaaaca atataaaatc agttaatctg ataaattttg agtaaataat aaagttaact 540ttgtacttac ctatatcaaa ctaattcaca aaataaagta ataataacaa agaattttta 600gtagatccac aatatacaca cacactatga gaaatcataa tagagaattt taatgatttt 660gtctaactca tagcaacaag tcgctttggc cgagtggtta aggcgtgtgc ctgctaagta 720catgggctct gcccgcgaga gttcgaatct ctcaggcgac gtttcttttg ttttcggcca 780taaaggaaaa agcccaatta acacgtctcg cttataagcc cataaagcaa acaatgggct 840gtctctgtct cactcacaca cgcgttttcc tactttttga ctatttttat aaccggcggg 900tctgacttaa ttagggtttt ctttaataat cagacactct ctcactcgtt tcgtcaacat 960tgaacacaga caaaaccgcg t 98182982DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0080 as found in Promoter Report #106 82aagcggcaat ttagtaagaa gtattcaatg tatcatttac caaaagtata tggttttggg 60aagagttgtt agggatgtat tctttctaaa cagatgatat gacgatgttc ttgaaaacta 120atgttaaaga cgggatctct cgcatcttca ctcgggagat atattaaacc gttgattgta 180gttagccatg tacttagctt agtgcacaaa taatctgctg caagaaatct ttttctatta 240taatatctct catttaaaca ttagaacata ttgtttaact tgttcttcta gaaataaaac 300tgctaatttc ttatggtaaa ctattttcct ttagattgca caatcgaact cgaaaatcta 360gtggagacta tgtgactatg tttatatata tgaaacctaa atcaaattat cccaataatt 420gggagacaca aaagaaaaaa agaaaacagg aaatcaaatc aaaagataaa gagaaggtaa 480aacaaaaggc aagaagcact aatgtttaat atttatagtt ttctccatta aagaaaaagc 540gatgatgtgt gtgttctcat cttttgtgaa agtatatata ttgcttttgc tcttctcaaa 600agcaaaagac tcatccaaca acaacaacaa aaaaaaacct aaagctcaat ccaaaagacg 660aagaatgcat tggatactac aacttctttt tcacttttct ttcgaattta caattatgat 720tttcacaata cagtgtattc aaaaataaat aaaaaaacga ggcatgaaaa taatgattat 780cctcttcact tattaagcca ctcactataa gcagagcaac tccagaacat agtgagcccc 840caaaacatta aagcatgatg atgtctaatg atgatgatct tcttcgttcc atttctctaa 900atttttggga tttctgcgaa gacccttctt ctctttctct tctctgaact tcaagattcg 960tgtcggacaa atttttgttt tt 98283987DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0122 as found in Promoter Report #107 83agtttaatta tttgttatct atccaatcaa tttttttttc taaactgttt ggaccaatgt 60acgtacgtac catccttttt gatttttttt gtaaactaaa ttttcggatt agcaggttct 120taataattga acgaagaaaa taaagaatag aggtagacac ctgtagtatt ttcttggtca 180gaccaataat ttataattca acgtcaaaga agaagaaaaa tataaaccat tatttcatta 240tgacttacgt ataccaaaat acacaaatta aatgtataat tgtgaggcat tttatatgcg 300ggaaaaaata aaataaaaag aatattaata tttcttttga aaattgtaaa gcattttgac 360ccacttgtga tatatatata tagatatata tagagagaga gattaaaaca ttgatggcta 420gctatagagt ctatggcagg gtcatgatca cctatcttct gatctctgaa gagataccaa 480tctgattttt tctcttccta ggtttaattt tattttacca ttttataatt ctttattttt 540gcctgtagta caatttacag acccatacta aaagaaaaat taaattttgt caaagtacaa 600aacaaagaga gaggtgaagc cacacaatct cttttcttct ctctctctct gttatatctc 660ttctgtttaa ttcttttatt cttcttcgtc tatcttctcc tataatctct tctctctccc 720tcttcaccta aagaataaga agaaaaataa ttcacatctt tatgcaaact actttcttgt 780agggttttag gagctatctc tattgtcttg gttctgatac aaagttttgt aattttcatg 840gtatgagaar atttgccttt ctattttgtt tattggttct ttttaacttt ttcttggaga 900tgggttcttg tagatcttaa tgaaacttct gtttttgtcc caaaaagagt tttctttttt 960cttctcttct tttttggttt tcaattc 98784999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0226 as found in Promoter Report #108 84ctccttgata atgattttga tcaaaagtgt aatttccaca aaccaattgc gcctgcaaaa 60gttttcaaag gatcatcaaa cataatgatg aatatctcat caccacgatt ttataataat 120gcatcttttc ccaccatttt ttttccctca ctttctttta taatcttgtt cgacaacaat 180catggtctaa ggaaaaagtt gaaaatatat attatcttag ttattagaaa agaaagataa 240tcaaatggtc gatatgcaaa tggcatatga ccataaacga gtttgctagt ataaagaatg 300atggccaacc tgttaaagag agactaaaat taggtctaaa atctaggagc aatgtaacca 360atacatagta tatgaaatat aaaagttaat ttagattttt tgattagccc aaattaaaga 420aaaatggtat ttaaaacaga gactcttcat cctaaaggct aaagcaatac aatttttggt 480taagaaaaga aaaaaaccac aagcggaaaa gaaaacaaaa aaaactatat tatgatgcaa 540cagcaacaca aagcaacacc ttgcacacac acatacaact gtaaacaagt ttcttgggac 600tctctatttt ctcttgctgc ttgaaccaaa cacaacaacg atatcccaac gagagcacaa 660caggtttgat tatgtcggaa gacaagtttt gagagaaaac aaacaatatt ttataacaaa 720ggagaagact tttggttagg aaaaattggt atggccatta caagacatat gggtcccaat 780tctcatcact ctctccacca ccaaaatcct cctctctctc tctctctttt actctgtttt 840catcatctct ttctctcgtc tctctcaaac cctaaataca ctctttctct tcttgttgtc 900tccattctct ctgtgtcatc aagcttcttt tttgtgtggg ttatttgaaa gacactttct 960ctgctggtat cattggagtc tagggttttg ttattgaca 999851002DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0244 as found in Promoter Report #110 85ttgatttgat gtatagttac tatttaaagt cttatttgtg aaattttaca aatgttggaa 60aaaagcattt tatggtgcta tatttgtcag tttcccttga ttatatatcc ttttgaaaag 120taatgttttt tttatgtgtg tgtattcatg aaccttggaa aaactacaaa tcagatcatg 180gtctgtttta ggtgaaaaat ttagaacaca gttacgcaag aaagatatcg gtaaattttt 240gtttctttga atcgaaatta atcaaaaagt attttccatt atataacaac aactaatctc 300tgtttttttt tttttttttt aacaactaat ctcttatcaa aatgacacta cagaatcacg 360attgtaaatc ttcaaaaggg cagtctgaaa aaatattcat gaggatgaga ttttattcat 420tcatggttgt aagtaatcat tatgtaaagt ttaggataag gacgttcaaa atcatataaa 480aaaactctac gaataaagtt tatagtctat catattgatt catatttcat agaaagttac 540tggaaaacat tacacaagta ttctcgattt ttacgagttt gtttagtagt cgcaaaattt 600tattttactt ttgagtatac gaacccataa gctgattttc tttccaagtt ccaataatga 660tatcatagtg tactcttcat gaatgtttca agcatataat tataacgttc ataagtaaca 720ttctactgca tgtttgttat tataaattaa ctaataatcg aacgtatgag ttttggttga 780gattgttgtg ctcacgaaat gaaggactcg gtcaattcta aagcttaaaa taagaagctc 840agatcttaaa actcgctttc gtcttcgtcc tccatttaag tttgcgattc ttttgctctt 900ctttctctct cacatttttg tcccaaaaca ataaaaagaa acaataatag aaagtgttac 960agaaaaagaa agaaaactat cattgaagtt gggaaggaga aa 100286996DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0286 as found in Promoter Report #111 86gaaaacaatc ataggttacg ctattatcat cgaaaggtat gtgatgcata ttcccattga 60accagatttc catatatttt atttgtaaag tgataatgaa tcacaagatg attcaatatt 120aaaaatgggt aactcacttt gacgtgtagt acgtggaaga atagttagct atcacgcata 180catatatcta tgaataagtg tgtatgacat aagaaactaa aatatttacc taaagtccag 240ttactcatac tgatttcatg catatatgta ttatttattt atttttaata aagaagcgat 300tggtgttttc atagaaatca tgatagattg ataggtattt cagttccaca aatctagatc 360tgtgtgctat acatgcatgt attaattttt tccccttaaa tcatttcagt tgataatatt 420gctctttgtt ccaactttag aaaaggtatg aaccaacctg acgattaaca agtaaacatt 480aattaatctt tatatgagat aaaaccgagg atatatatga ttgtgttgct gtctattgat 540gatgtgtcga tattatgctt gttgtaccaa tgctcgagcc gagcgtgatc gatgccttga 600caaactatat atgtttcccg aattaattaa gttttgtatc ttaattagaa taacattttt 660atacaatgta atttctcaag cagacaagat atgtatccta tattaattac tatatatgaa 720ttgccgggca cctaccagga tgtttcaaat acgagagccc attagtttcc acgtaaatca 780caatgacgcg acaaaatcta gaatcgtgtc aaaactctat caatacaata atatatattt 840caagggcaat ttcgacttct cctcaactca atgattcaac gccatgaatc tctatataaa 900ggctacaaca ccacaaagga tcatcagtca tcacaaccac attaactctt caccactatc 960tctcaatctc tcgtttcatt tcttgacgcg tgaaaa 99687998DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0289 as found in Promoter Report #112 87atggactttt cttctattat atggtcaaac aattactgct caatgtattt gcgtatagag 60catgtccaat accatgcctc atgatgtgag attgcgaggc ggagtcagag aacgagttaa 120agtgacgacg tttttttgtt ttttttgggc atagtgtaaa gtgatattaa aatttcatgg 180ttggcaggtg actgaaaata aaaatgtgta taggatgtgt ttatatgcta gacggaaaaa 240tagttactca actaatacag atctttataa agagtatata agtctatggt taatcatgaa 300tggcaatata taagagtaga tgagatttat gtttatattg aaacaaggga aagatatgtg 360taattgaaac aatggcaaaa tatatagtca aatcaaactg gtttctgata atatatgtgt 420tgaatcaatg tatatcttgg tattcaaaac caaaacaact acaaccaatt tctttaaaaa 480accagttgat ctaataacta cattttaata ctagtagcta ttagctgtat ttcataatca 540atttcttgca ttaaaatttg aagtgggttt tgcatttaaa cttactcggt ttgtattaat 600agactttcaa agattaaaag aaaactactg cattcagaga ataaagctat cttactaaac 660actactttta aagtttcttt tttcacttat taatcttctt atacaaatgg atctgtctct 720ctgcatggca aaatacttac actaatttta ttttctttgt ttgataacaa atttatcggc 780taagcatcac ttaaatttaa tacacgttat gaagactaaa accacgtcac actataagaa 840ccttacaggc tgtcaaacac ccttccctac ccactcacat ctctccacgt ggcaatcttt 900gatattgaca ccttagccac tacagctgtc acactcctct ctcggtttca aaacaacatc 960tctggtataa atacctctgt atatctttat aaacccca 99888984DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0015 as found in Promoter Report #116 88ttgagcctta ttgttgttat tgacttttag ccaatagaaa gagatggaaa ttcaataatt 60atccacaaaa ttccaaatca ttggtgtaca aaaagatcta aggctgttat atttccaaaa 120aagaaagaaa agaaatgcaa caaatatgga ttaaactgtg gtttgtaaat tgagctttgc 180atgaaaactt tatcactatg atttcactac tccatattta ttgactaaag tggcactaat 240gaatttctta atcatgaaat cttgtatcaa aaagtactaa aataaacatg acattggcaa 300ttaggaaaat tctaaattag aaattagtaa aaatgaaagg tgaaagggaa agatgatgat 360atgaattggt tggtgaccag gagaaatgta tcccgatttt tgcagacact ttcagtgtcc 420ccattcatat aattatggcc cacctcgtta agatttttca ttcaccacca taacaagatc 480taagcttaga tttcatgtaa ttaaacatat aatatacttg ccaatactat ctaataaagt 540atacttaagc aaaaattatt actctagtgt aaggcgatga aatataagtt tagttgaaaa 600tttatgtcga tataacaaag tataatgaat taagaccttg gttttcgatt aacaaactaa 660ttaaacacta gttttgccta ataaaaccgg gaatcgtatt caaaaccgaa cgacaaaaca 720agggacaagt tgagagacaa aaccaaatca gcatctttct tccagaaatg tcatgaccac 780atgacgtcat cttgaccctt cttcattgtg atatctgtgg ataaagcgca cgtgtttaat 840tcacgaacct tcgtagtaac gaaaaatcca caactttcat attttttaat tacccactaa 900actaaaacaa atttggaaaa acatgaaaaa ctttttcttt ttttccaggt tcgtgaacct 960cgtaccctct atataaacct ctta 98489970DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0230 as found in Promoter Report #118 89aatcattaaa tctttgatga gaaatatcca atctactaat gtatatcgat gatttaaatg 60aaattactta tttgaacaca aaaataaatg aatttactaa taaataaata gcgtagttgc 120gagcaagtgg ctaaaaaaat tacaaatcta gtttccattc tcagcggcgg ggtgcttgga 180acgtcaccgt tttttggaaa acgcaatctt cctcccttcc gggacgtctc accggaattt 240tctcgctttt gtctactctc ctccatctcc gaggttctcc aagctcagct cctcttccca 300tcattcatcc gaccgcctta tccggtcaga tcctttacgt atttctattt tcctgatcgt 360cgatttttga gaaatgtaaa aacagatcgt ataaggcctc gaagttttta atttgaaagt 420ggtatcgaaa ttttttggtc tttgattagg ttagggcacc gtagctctgg gtattgaatt 480tgtagggttt tcctctggtt attggtcttt ggagcttggt aatttctgct gaattgattg 540atcccttttc catcttttga agtaaagtct cgagctttcg tgtctcgatg tagatgaatt 600ctattttgaa tatgagattt gataagacgt caattgctga taatttggag tctttgtgtc 660tgaatttgtt catatgaagt tttctgaggg atgtgaattt tattgtctgc taattttgaa 720acgttccttt tggaatttgg tttgtgagga gtcctagatc tttttctgtt aagtttcttg 780cttgtaagtt ttctggatca cttgattgag tctagaatct agatagatta catgttcggt 840ttgattcctt tggctgattt tccaaagttt tgttcaaatt tcaggagaac tacaaagagg 900aaaccaagat ggttttgttt tgttagactc tacccctttt ccgattcaca tggtaaggac 960attgaggtag 97090999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0120 as found in Promoter Report #119 90tagtttttga tttaatctac gtttttctta atcataaatg ggtaattatt agtttttgca 60aaatcaaaat ccaaaaattg ttctaaacac tgcaaccatt taaggcctat atcactcaga 120aaatttctgg tgggagaact aatcgtttgt cctttctaaa tctcacatat tagaatttag 180aattagtgtg ctacataaga atattagttc agctcggaac aactattttt tggtaaaaca 240gagaacttaa acaaatgcat tattttatca acatgcattt tgaattgaat ataaaatttc 300ataattgtaa agacataaat tacataaaat tttacatgaa aaaatagata tagaaagaaa 360atgaaactaa ctgatgatat gctctctaaa ttttttaatc tcataacaag aattcaaatt 420aattagttca tatttttggt taatataaca tttacctgtc taagttggaa ctttcatttt 480tttctgtttt gtttagtcag tattcttaat gtgaaacgga aagttgaatt tattcaaact 540taaattcaat agcattaatt aaaggcgaaa gctattatct ctacatgtgg ttcaaactag 600acatccaatt taattagctt attgacgttg aaatgttttc caaaactact atagtttggc 660aatttgaaag atgcatcaga actactcaga caggtaaaag tagaacctct agctgtgtga 720attgtatgtt agtccataaa gaacatcttg taaacttcat acttaagata tatattacaa 780tatatacttg aatggtagat aaaaacgatt agtctgattg ctagcatact cacaactatt 840tggaaatgag taagatattg gcattctaga gttactacta tggagacaaa agtcgaataa 900aagagacctc acgtgaaaat gttacgagct agtaaaaaaa gcatttacac taacggtaaa 960aaaagtatct ataaatgttt acacaaggta gtagtcatt 99991989DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0261 as found in Promoter Report #120 91ttgtaaatta gttttatcgt agaagtacca aatcaagtga ttcaatggtt aaattaaggt 60attaagttac atttgatatt taaaagtatc cagaccttca ttatagctca taagggttaa 120aattttgtcg ttcttttgta

tattcatggc aagctctaat tcatgactaa gtcacatttt 180tcaaatatgt ttttagtttt tacttatgtt ggtaattagt ggatttatag ttaagttaaa 240aagttggcga gttctagctt tgaaactcat ttagaaatat atatatatat atatatattc 300aattttagta aattgttaat ctattctaat ggtgtaactg taacaaatga gaatgaaaaa 360aatatactat tgtgaataaa accccacaca acacattact ataataagtt aaacttcttt 420ttttataggc gcctggaaaa aaaagaaaag caacaagagg gstgtgagga cgcatcaccn 480ggtttcgtag cacacatgtg catttgtctc tttgcttttt cggttttttt cttgccaatc 540aatttatttt gttcctcaga aaaaagaaaa tctaaaacca aaatatatat tataacctca 600tttaataaac aacaaaaatg tttgttgaaa aaaaaaaagt ttttatttat cttgacctta 660tttctttgaa gaaaataaag cttggttatt aaagaagtcc aagttagttg ccaccatcag 720tggcataacg gtaaattaaa gccaacttcc tctaactaaa gttttctata aattcaacca 780ctcacctccc actctaaaac ccaacaacat aatttcacat atctctcttt ctttctcttg 840aaggaaagac gaagatctcc aagtcccaag tacgtaacta ctttctccat ctacattcaa 900ttgtttctcc ttaatttctc tagtacatat ttacttgtgc tataagtaat tgattttata 960tcacccatgt gcaggttgtt aacacaaga 98992911DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0263 as found in Promoter Report #121 92atctagctgt ggattccacc aaaattctgg cagggccatg atctaaaaac tgagactgcg 60cgtgttgttt tgcagtgatt tgtatttcat atttgcacca tcctacacag tccacttggt 120atcgtaacca aacataagga gaacctaatt acattattgt tttaatttcg tcaaactggt 180ttttaccttt tagttacata gttgattctt catttgtttt agtagttatg gagcacaata 240atgtgcaaca aagaaagatc atagtggatt aatatgttga gaggtcagaa attcttggtt 300aacaaaaaaa agttacaagg actgagattt tgggtgggag aaagccatag cttttaaaac 360atgattgaac ttaaaagtga tgttatggtt tgaggggaaa aaggttgatg tcaactaaga 420tagttgaagt aatgtcttaa actaaagtaa accaccggtc caaccgtggt ccggaagcat 480ctctggtatg atttatccta aaaatcaaaa tagtagaaac atactttaaa tatatacatt 540gatcggacga aaattgtaaa ctagtatagt ttcaaaaact agttgaacag gttatgtacc 600ttaaacattt atttcaaact taaacactaa agaacatata tgaatagaag tttatataaa 660ttactatata tctaccataa atctcttata attatgatgt cacgatgagg aagtgttgaa 720acgttaaaat gccaaaatat aagcatgcga cggaattttg gcagaagatt gtagagttgt 780aatctgtcgc aatcattact cgtgctagca tttttcattt tcccttcatt tgtggataac 840gcacgatata acattctaca caccaacaag attctataaa aacgcaaagg ttgtctccat 900agaatatcgt c 91193863DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0003 as found in Promoter Report #123 93tggatctgct agatatatga gaacgaaaga accagaagct attagaggcg ggaggagata 60tgtggggatg atttcagtgc aattccacga cgcaccattt ccactttcgt aacacctaaa 120cgaccgcttc ggccgtataa aatcgcaaat gtttggtctc agtgtatttt tccaatttcc 180aaatacatca attcaaatta tataatatct agtggcaatt ataagtatat catatatttt 240caaaattaat taaaaagatt actaaattat gtttgactac aactattata atagttaaaa 300acataaacaa aaacaaagaa actattttaa taaaaaaatc aagtaaacat taaaacataa 360gcaaaaaata atgttaaaga aattattaat tattaattta ctaataatta atacctctat 420aaattaattg ttagaggttt aacgtaattt ataaggaaaa ctaaagaaga ctttaaccca 480taaagaaaaa aacaaagact gaattgaagg cccatattta gaagaagaga aagaagaccc 540aaatatgata taaaatccag cccatttata tatttttatt ttgtttctgg aaggaaaata 600agaaaatggc aaaaacgaaa taatctgaaa aagtaaggtc ttttaccaaa aaggatattt 660tttttataaa cagagcataa agttttcact tttcttctgc tcctttctcg tctctgtctt 720cttcgtcctc attcgtttta aagcatcaaa atttcatcaa cccaaaatag attaaaaaaa 780tctgtagctt tcgcatgtaa atctctcttt gaaggttcct aactcgttaa tcgtaactca 840cagtgactcg ttcgagtcaa agt 863941000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0356 as found in Promoter Report #125 94ttagttcatt gaaacgtcaa ctttttactt gcaaccactt tgtaggacca ttaactgcaa 60aataagaatt ctctaagctt cacaaggggt tcgtttggtg ctataaaaac attgttttaa 120gaactggttt actggttcta taaatctata aatccaaata tgaagtatgg caataataat 180aacatgttag cacaaaaaat actcattaaa ttcctaccca aaaaaaatct ttatatgaaa 240ctaaaactta tatacacaat aatagtgata caaagtaggt cttgatattc aactattcgg 300gattttctgg tttcgagtaa ttcgtataaa aggtttaaga tctattatgt tcactgaaat 360cttaactttg ttttgtttcc agttttaact agtagaaatt gaaattttta aaaattgtta 420cttacaataa aatttgaatc aatatcctta atcaaaggat cttaagacta gcacaattaa 480aacatataac gtagaatatc tgaaataact cgaaaatatc tgaactaagt tagtagtttt 540aaaatataat cccggtttgg accgggcagt atgtacttca atacttgtgg gttttgacga 600ttttggatcg gattgggcgg gccagccaga ttgatctatt acaaatttca cctgtcaacg 660ctaactccga acttaatcaa agattttgag ctaaggaaaa ctaatcagtg atcacccaaa 720gaaaacattc gtgaataatt gtttgctttc catggcagca aaacaaatag gacccaaata 780ggaatgtcaa aaaaaagaaa gacacgaaac gaagtagtat aacgtaacac acaaaaataa 840actagagata ttaaaaacac atgtccacac atggatacaa gagcatttaa ggagcagaag 900gcacgtagtg gttagaaggt atgtgatata attaatcggc ccaaatagat tggtaagtag 960tagccgtcta tatcatccat actcatcata acttcaacct 1000951000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0374 as found in Promoter Report #126 95aagacacccg taaatgttgt catgtagaag aaactagaaa cgttaaacgc atcaaatcaa 60gaaattaaat tgaaggtaat ttttaacgcc gcctttcaaa tattcttcct aggagaggct 120acaagacgcg tatttctttc gaattctcca aaccattacc attttgatat ataataccga 180catgccgttg ataaagtttg tatgcaaatc gttcattggg tatgagcaaa tgccatccat 240tggttcttgt aattaaatgg tccaaaaata gtttgttccc actactagtt actaatttgt 300atcactctgc aaaataatca tgatataaac gtatgtgcta tttctaatta aaactcaaaa 360gtaatcaatg tacaatgcag agatgaccat aaaagaacat taaaacacta cttccactaa 420atctatgggg tgccttggca aggcaattga ataaggagaa tgcatcaaga tgatatagaa 480aatgctattc agtttataac attaatgttt tggcggaaaa ttttctatat attagacctt 540tctgtaaaaa aaaaaaaatg atgtagaaaa tgctattatg tttcaaaaat ttcgcactag 600tataatacgg aacattgtag tttacactgc tcattaccat gaaaaccaag gcagtatata 660ccaacattaa taaactaaat cgcgatttct agcaccccca ttaattaatt ttactattat 720acattctctt tgcttctcga aataataaac ttctctatat cattctacat aataaataag 780aaagaaatcg acaagatcta aatttagatc tattcagctt tttcgcctga gaagccaaaa 840ttgtgaatag aagaaagcag tcgtcatctt cccacgtttg gacgaaataa aacataacaa 900taataaaata ataaatcaaa tatataaatc cctaatttgt ctttattact ccacaatttt 960ctatgtgtat atatataccc acctctctct tgtgtatttg 100096998DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0377 as found in Promoter Report #127 96tataaaccat tcctataaca ccatatttaa acataacaat gaattgcttg gatttcaaac 60tttattaaat ttggatttta aattttaatt tgattgaatt ataccccctt aattggataa 120attcaaatat gtcaactttt tttttgtaag atttttttat ggaaaaaaaa attgattatt 180cactaaaaag atgacaggtt acttataatt taatatatgt aaaccctaaa aagaagaaaa 240tagtttctgt tttcacttta ggtcttatta tctaaacttc tttaagaaaa tcgcaataaa 300ttggtttgag ttctaacttt aaacacatta atatttgtgt gctatttaaa aaataattta 360caaaaaaaaa aacaaattga cagaaaatat caggttttgt aataagatat ttcctgataa 420atatttaggg aatataacat atcaaaagat tcaaattctg aaaatcaaga atggtagaca 480tgtgaaagtt gtcatcaata tggtccactt ttctttgctc tataacccaa aattgaccct 540gacagtcaac ttgtacacgc ggccaaacct ttttataatc atgctattta tttccttcat 600ttttattcta tttgctatct aactgatttt tcattaacat gataccagaa atgaatttag 660atggattaat tcttttccat ccacgacatc tggaaacact tatctcctaa ttaaccttac 720ttttttttta gtttgtgtgc tccttcataa aatctatatt gtttaaaaca aaggtcaata 780aatataaata tggataagta taataaatct ttattggata tttctttttt taaaaaagaa 840ataaatcttt tttggatatt ttcgtggcag catcataatg agagactacg tcgaaaccgc 900tggcaaccac ttttgccgcg tttaatttct ttctgaggct tatataaata gatcaaaggg 960gaaagtgaga tataatacag acaaaacaag agaaaaga 99897999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0380 as found in Promoter Report #128 97acaagtacca ttcacttttt tacttttcaa tgtatacaat catcatgtga taaaaaaaaa 60aatgtaacca atcaacacac tgagatacgg ccaaaaaatg gtaatacata aatgtttgta 120ggttttgtaa tttaaatact ttagttaagt tatgatttta ttatttttgc ttatcactta 180tacgaaatca tcaatctatt ggtatctctt aatcccgctt tttaatttcc accgcacacg 240caaatcagca aatggttcca gccacgtgca tgtgaccaca tattgtggtc acagtactcg 300tccttttttt ttcttttgta atcaataaat ttcaatccta aaacttcaca cattgagcac 360gtcggcaacg ttagctccta aatcataacg agcaaaaaag ttcaaattag ggtatatgat 420caattgatca tcactacatg tctacataat taatatgtat tcaaccggtc ggtttgttga 480tactcatagt taagtatata tgtgctaatt agaattagga tgaatcagtt cttgcaaaca 540actacggttt catataatat gggagtgtta tgtacaaaat gaaagaggat ggatcattct 600gagatgttat gggctcccag tcaatcatgt tttgctcgca tatgctatct tttgagtctc 660ttcctaaact catagaataa gcacgttggt tttttccacc gtcctcctcg tgaacaaaag 720tacaattaca ttttagcaaa ttgaaaataa ccacgtggat ggaccatatt atatgtgatc 780atattgcttg tcgtcttcgt tttcttttaa atgtttacac cactacttcc tgacacgtgt 840ccctattcac atcatccttg ttatatcgtt ttacttataa aggatcacga acaccaaaac 900atcaatgtgt acgtcttttg cataagaaga aacagagagc attatcaatt attaacaatt 960acacaagaca gcgagattgt aaaagagtaa gagagagag 999981000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0381 as found in Promoter Report #129 98cacggtcaaa gtattgctaa catggtcatt acattgaaaa agaaaattaa ttgtctttac 60tcatgtttat tctatacaaa taaaaatatt aaccaaccat cgcactaaca aaatagaaat 120cttattctaa tcacttaatt gttgacaatt aaatcattga aaaatacact taaatgtcaa 180atattcgttt tgcatacttt tcaatttaaa tacatttaaa gttcgacaag ttgcgtttac 240tatcatagaa aactaaatct cctaccaaag cgaaatgaaa ctactaaagc gacaggcagg 300ttacataacc taacaaatct ccacgtgtca attaccaaga gaaaaaaaga gaagataagc 360ggaacacgtg gtagcacaaa aaagataatg tgatttaaat taaaaaacaa aaacaaagac 420acgtgacgac ctgacgctgc aacatcccac cttacaacgt aataaccact gaacataaga 480cacgtgtacg atcttgtctt tgttttctcg atgaaaacca cgtgggtgct caaagtcctt 540gggtcagagt cttccatgat tccacgtgtc gttaatgcac caaacaaggg tactttcggt 600attttggctt ccgcaaatta gacaaaacag ctttttgttt gattgatttt tctcttctct 660ttttccatct aaattctctt tgggctctta atttcttttt gagtgttcgt tcgagatttg 720tcggagattt tttcggtaaa tgttgaaatt ttgtgggatt tttttttatt tctttattaa 780actttttttt attgaattta taaaaaggga aggtcgtcat taatcgaaga aatggaatct 840tccaaaattt gatattttgc tgttttcttg ggatttgaat tgctctttat catcaagaat 900ctgttaaaat ttctaatcta aaatctaagt tgagaaaaag agagatctct aatttaaccg 960gaattaatat tctccgaccg aagttattat gttgcaggct 100099999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0382 as found in Promoter Report #130 99gcaaacaata atttatcgta agagtttttt taaaattcgt tggaacttgg aagggatttt 60aaatattatt ttgttttcct tcatttttat aggttaataa ttgtcaaaga tacaactcga 120tggaccaaaa taaaataata aaattcgtcg aatttggtaa agcaaaacgg tcgaggatag 180ctaatattta tgcgaaaccc gttgtcaaag cagatgttca gcgtcacgca catgccgcaa 240aaagaatata catcaacctc ttttgaactt cacgccgttt tttaggccca caataatgct 300acgtcgtctt ctgggttcac cctcgttttt tttttaaact tctaaccgat aaaataaatg 360gtccactatt tcttttcttc tctgtgtatt gtcgtcagag atggtttaaa agttgaaccg 420aactataacg attctcttaa aatctgaaaa ccaaactgac cgattttctt aactgaaaaa 480aaaaaaaaaa aaactgaatt taggccaact tgttgtaata tcacaaagaa aattctacaa 540tttaattcat ttaaaaataa agaaaaattt aggtaacaat ttaactaagt ggtctatcta 600aatcttgcaa attctttgac tttgaccaaa cacaacttaa gttgacagcc gtctcctctc 660tgttgtttcc gtgttattac cgaaatatca gaggaaagtc cactaaaccc caaatattaa 720aaatagaaac attactttct ttacaaaagg aatctaaatt gatccctttc attcgtttca 780ctcgtttcat atagttgtat gtatatatgc gtatgcatca aaaagtctct ttatatcctc 840agagtcaccc aatcttatct ctctctcctt cgtcctcaag aaaagtaatt ctctgtttgt 900gtagttttct ttaccggtga attttctctt cgttttgtgc ttcaaacgtc acccaaatca 960ccaagatcga tcaaaatcga aacttaacgt ttcagaaga 9991001000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0388 as found in Promoter Report #131 100agaagtattc acgcaccaag gttatatttg tagtgacata ttctacaatt atcacatttt 60tctcttatgt ttcgtagtcg cagatggtca attttttcta taataatttg tccttgaaca 120caccaaactt tagaaacgat gatatatacc gtattgtcac gctcacaatg aaacaaacgc 180gatgaatcgt catcaccagc taaaagccta aaacaccatc ttagttttca ctcagataaa 240aagattattt gtttccaacc tttctattga attgattagc agtgatgacg taattagtga 300tagtttatag taaaacaaat ggaagtggta ataaatttac acaacaaaat atggtaagaa 360tctataaaat aagaggttaa gagatctcat gttatattaa atgattgaaa gaaaaacaaa 420ctattggttg atttccatat gtaatagtaa gttgtgatga aagtgatgac gtaattagtt 480gtatttatag taaaacaaat taaaatggta aggtaaattt ccacaacaaa acttggtaaa 540aatcttaaaa aaaaaaaaag aggtttagag atcgcatgcg tgtcatcaaa ggttcttttt 600cactttaggt ctgagtagtg ttagactttg attggtgcac gtaagtgttt cgtatcgcga 660tttaggagaa gtacgtttta cacgtggaca caatcaacgg tcaagatttc gtcgtccaga 720tagaggagcg atacgtcacg ccattcaaca atctcctctt cttcattcct tcattttgat 780tttgagtttt gatctgcccg ttcaaaagtc tcggtcatct gcccgtaaat ataaagatga 840ttatatttat ttatatcttc tggtgaaaga agctaatata aagcttccat ggctaatctt 900gtttaagctt ctcttcttct tctctctcct gtgtctcgtt cactagtttt ttttcggggg 960agagtgatgg agtgtgtttg ttgaatagtt ttgacgatca 10001011000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0396 as found in Promoter Report #133 101catagtaaaa gtgaatttaa tcatactaag taaaataaga taaaacatgt tatttgaatt 60tgaatatcgt gggatgcgta tttcggtatt tgattaaagg tctggaaacc ggagctccta 120taacccgaat aaaaatgcat aacatgttct tccccaacga ggcgagcggg tcagggcact 180agggtcattg caggcagctc ataaagtcat gatcatctag gagatcaaat tgtatgtcgg 240ccttctcaaa attacctcta agaatctcaa acccaatcat agaacctcta aaaagacaaa 300gtcgtcgctt tagaatgggt tcggtttttg gaaccatatt tcacgtcaat ttaatgttta 360gtataatttc tgaacaacag aattttggat ttatttgcac gtatacaaat atctaattaa 420taaggacgac tcgtgactat ccttacatta agtttcactg tcgaaataac atagtacaat 480acttgtcgtt aatttccacg tctcaagtct ataccgtcat ttacggagaa agaacatctc 540tgtttttcat ccaaactact attctcactt tgtctatata tttaaaatta agtaaaaaag 600actcaatagt ccaataaaat gatgaccaaa tgagaagatg gttttgtgcc agattttagg 660aaaagtgagt caaggtttca catctcaaat ttgactgcat aatcttcgcc attaacaacg 720gcattatata tgtcaagcca attttccatg ttgcgtactt ttctattgag gtgaaaatat 780gggtttgttg attaatcaaa gagtttgcct aactaatata actacgactt tttcagtgac 840cattccatgt aaactctgct tagtgtttca tttgtcaaca atattgtcgt tactcattaa 900atcaaggaaa aatatacaat tgtataattt tcttatattt taaaattaat tttgatgtat 960taccccttta taaataggct atcgctacaa caccaataac 10001021000DNAArabidopsis thalianamisc_featureCeres Promoter construct PT0506 as found in Promoter Report #135 102aggacttcca ctactctttc ccacacgctc cagaccactg tttgctttcc tctgattaac 60caatctcaat taaactacta atttataatt caagataatt agataaccaa tcttaaaatt 120tggaatcttc ttccctcact tgatattaca aaaaaaaaac tgatttatca tacggttaat 180tcaagaaaac agcaaaaaaa ttgcactata atgcaaaaca tcaattaatt acattcgatt 240aaaaaatcat cattgaatct aaaatggcct caaatctatt gagcatttgt catgtgccta 300aaatggttca ggagttttac atctaatcac ataaaaagca aacaataacc aaaaaaattg 360cattttagca aatcaaatac ttatatatat acgtatgatt aagcgtcatg actttaaaac 420ctctgtaaaa ttttgattta tttttcgatg cttttatttt ttaaccaata gtaataaagt 480ccaaatctta aatacgaaaa aatgtttctt tctaagcgac caacaaaatg gtccaaatca 540cagaaaatgt tccataatcc aggcccatta agctaatcac caagtaatac attacacgtc 600accaattaat acattacacg tacggccttc tctcttcacg agtaatatgc aaacaaacgt 660acattagctg taatgtactc actcatgcaa cgtcttaacc tgccacgtat tacgtaatta 720caccactcct tgttcctaac ctacgcattt cactttagcg catgttagtc aaaaaacaca 780aacataaact acaaataaaa aaactcaaaa caaaacccaa tgaacgaacg gaccagcccc 840gtctcgattg atggaacagt gacaacagtc ccgttttctc gggcataacg gaaacggtaa 900ccgtctctct gtttcatttg caacaacacc atttttataa ataaaaacac atttaaataa 960aaaattatta aaacctcaaa aaatctctgt ttcttgttta 10001031000DNAArabidopsis thalianamisc_featureCeres Promoter construct PT0511 as found in Promoter Report #136 103tttaattttg attctaaaga gttgtgacgg gtcatcacag attcttcgtt tttttataga 60tagaaaagga ataacgttaa aagtatacaa attatatgca agagtcattc gaaagaatta 120aataaagaga tgaactcaaa agtgatttta aattttaatg ataagaatat acatctcaca 180gaaatctttt atttgacatg taaaatcttg ttttcaccta tcttttgtta gtaaacaaga 240atatttaatt tgagcctcac ttggaacgtg ataataatat acatcttatc ataattgcat 300attttgcgga tagtttttgc atggggagat taaaggctta ataaagcctt gaatttccga 360ggggaggaat catgttttat acttgcaaac tatacaacca tctgcatcga taattggtgt 420taatacatgc aaggattata cactaaaaca aatcatttat ttccttacaa aaagagagtc 480gactgtgagt cacattctgt gacaaggaaa ggtcaagaac catcgctttt atcatcattc 540tctttgctaa caacttacaa ccacacaaac gcaagagttc cattctcatg gagaagaaca 600tattatgcaa aataatgtat gtcgatcgat agagaaaagg atccacaatt attgctccat 660ctcaaaagct tctttagtac acgatacatg tatcatgtaa atagaaatat gaaagataca 720atacacgacc cattctcata aagatagcaa catttcatgt tatgtaaaga gtcttcctta 780ggacacatgc attaaaacta aggattacca acccacttac tcctcactcc aaccaaatat 840caatcatcta ttttgggtcc ttcactcata agtcaactct catgccttcc tctataaata 900ccgtacccta cgcatccctt agttctacat cacataaaaa caatcatagc aaaaacatat 960atcctcaaat taattagatc tcatctatct ctaccctcga 1000104999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0262 as found in Promoter Report #141 104tacttcgtaa ggttaatata tgatatagtg ggcctgacaa aaaatcatat gggcttgaca 60aaatctatgt ttatatccag ttcggtccaa aattcgaaaa cataaacaat gtggaaagat 120gtgttggcta atctgatacc atgtcaaaaa aacatagaag ttcttgtgag gtttcttgtt 180tattcatgtg gaggcaattg cccttgtata caaggcttgt gaaagaagta aagaagataa 240gtttaggaag aaggtggccc atgagggccc aatattcata tcattagtga aaagcatagg 300aatttcaact tgtaatatta aaatcagtga taattgaata acattaatct ttttaagatt 360tccaaattaa ttcaaatctt cattaagaaa taagatttag gaatactaaa aagctaaata 420attatctaat tacttaaaat cagtaatgaa aattaatatt atactcccat agtatatttt 480cttcactatg aatgaagttg agacttcaat tttgacaaat tcggtgttct ggaataacaa 540aagaagtcaa agaaagaaaa ttagggagat agagtaaatt aaagttgaga caaatcagct 600tcattttgtt tttcccaaat aataatatac agatagatct ttcggccccg gcgcattctc 660tagatttgct catcaccacc tcgaaatttc tgctcgaagt tctccaaaac agttcattca 720tctacattta ccagatcata ttcgtattca gtcgtctcta aattctcact aaatcaatcc 780ttcctttgga taattccggt gtttgcgagg aatctgttga

aatgattggt tagttcagtt 840aaactctact tctaaaacta aatctgcatt caaatgtagt ctctagttct cgtacttgaa 900gtagacttga ttcgatttga tttactgatt cgtggctttt gatttcacag gtcaattgga 960aatctgagaa tccgtgtgac tattattgca gctggatcg 999105999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0275 as found in Promoter Report #142 105aaacattaat atgtagtaac tatgggcgta tgctttactt tttaaaatgg gcctatgcta 60taattgaatg acaaggatta aacaactaat aaaattgtag atgggttaag atgacttatt 120tttttactta ccaatttata aatgggcttc gatgtactga aatatatcgc gcctattaac 180gaggccattc aacgaatgtt ttaagggccc tatttcgaca ttttaaagaa cacctaggtc 240atcattccag aaatggatat tataggattt agataatttc ccacgtttgg tttatttatc 300tattttttga cgttgaccaa cataatcgtg cccaaccgtt tcacgcaacg aatttatata 360cgaaatatat atatttttca aattaagata ccacaatcaa aacagctgtt gattaacaaa 420gagatttttt ttttttggtt ttgagttaca ataacgttag aggataaggt ttcttgcaac 480gattaggaaa tcgtataaaa taaaatatgt tataattaag tgttttattt tataatgagt 540attaatataa ataaaacctg caaaaggata gggatattga ataataaaga gaaacgaaag 600agcaatttta cttctttata attgaaatta tgtgaatgtt atgtttacaa tgaatgattc 660atcgttctat atattgaagt aaagaatgag tttattgtgc ttgcataatg acgttaactt 720cacatataca cttattacat aacatttatc acatgtgcgt cttttttttt ttttactttg 780taaaatttcc tcacttttaa gacttttata acaattacta gtaaaataaa gttgcttggg 840gctacaccct ttctccctcc aacaactcta tttatagata acattatatc aaaatcaaaa 900catagtccct ttcttctata aaggtttttt cacaaccaaa tttccattat aaatcaaaaa 960ataaaaactt aattagtttt tacagaagaa aagaaaaca 9991061000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0337 as found in Promoter Report #143 106taattttttt atttttggaa ctaacactta ttagtttagg tttccatcac ctatttaatt 60cgtaattctt atacatgcat ataatagaga tacatatata caaatttatg atcatttttg 120cacaacatgt gatctcattc attagtatgc attatgcgaa aacctcgacg cgcaaaagac 180acgtaatagc taataatgtt actcatttat aatgattgaa gcaagacgaa aacaacaaca 240tatatatcaa attgtaaact agatatttct taaaagtgaa aaaaaacaaa gaaatataaa 300ggacaatttt gagtcagtct cttaatatta aaacatatat acataaataa gcacaaacgt 360ggttacctgt cttcatgcaa tgtggacttt agtttatcta atcaaaatca aaataaaagg 420tgtaatagtt ctcgtcattt ttcaaatttt aaaaatcaga accaagtgat ttttgtttga 480gtattgatcc attgtttaaa caatttaaca cagtatatac gtctcttgag atgttgacat 540gatgataaaa tacgagatcg tctcttggtt ttcgaatttt gaactttaat agttttcttt 600tttagggaaa ctttaatagt tgtttatcat aagattagtc acctaatggt tacgttgcag 660taccgaacca attttttacc cttttttcta aatgtggtcg tggcataatt tccaaaagag 720atccaaaacc cggtttgctc aactgataag ccggtcggtt ctggtttgaa aaacaagaaa 780taatctgaaa gtgtgaaaca gcaacgtgtc tcggtgtttc atgagccacc tgccacctca 840ttcacgtcgg tcattttgtc gtttcacggt tcacgctcta gacacgtgct ctgtccccac 900catgactttc gctgccgact cgcttcgctt tgcaaactca aacatgtgtg tatatgtaag 960tttcatccta ataagcatct cttaccacat taattaaaaa 1000107999DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0384 as found in Promoter Report #144 107tttaaaaaat tggataaaac accgataaaa attcacattt gcaaatttta ttcagtcgga 60atatatattt gaaacaagtt ttgaaatcca ttggacgatt aaaattcatt gttgagagga 120taaatatgga tttgttcatc tgaaccatgt cgttgattag tgattgacta ccatgaaaaa 180tatgttatga aaagtataac aacttttgat aaatcacatt tattaacaat aaatcaagac 240aaaatatgtc aacaataata gtagtagaag atattaattc aaattcatcc gtaacaacaa 300aaaatcatac cacaattaag tgtacagaaa aaccttttgg atatatttat tgtcgctttt 360caatgatttt cgtgaaaagg atatatttgt gtaaaataag aaggatcttg acgggtgtaa 420aaacatgcac aattcttaat ttagaccaat cagaagacaa cacgaacact tctttattat 480aagctattaa acaaaatctt gcctattttg cttagaataa tatgaagagt gactcatcag 540ggagtggaaa atatctcagg atttgctttt agctctaaca tgtcaaacta tctagatgcc 600aacaacacaa agtgcaaatt cttttaatat gaaaacaaca ataatatttc taatagaaaa 660ttaaaaaggg aaataaaata tttttttaaa atatacaaaa gaagaaggaa tccatcatca 720aagttttata aaattgtaat ataatacaaa cttgtttgct tccttgtctc tccctctgtc 780tctctcatct ctcctatctt ctccatatat acttcatctt cacacccaaa actccacaca 840aaatatctct ccctctatct gcaaattttc caaagttgca tcctttcaat ttccactcct 900ctctaatata attcacattt tcccactatt gctgattcat ttttttttgt gaattatttc 960aaacccacat aaaaaaatct ttgtttaaat ttaaaacca 9991081000DNAArabidopsis thalianamisc_featureCeres Promoter construct PT0535 as found in Promoter Report #145 108tatcattatt gtactgcctg tcaatttttt gattgtatct tttattcagc agcatcacat 60tctccttcag tgtccttctc ttcttttctt tttcttttct ttttctttcc tcattaaacc 120catacaattc tacggtaaat attatggatt tgcatacaaa ctctctaatt gtttgtttgg 180gtactataat atggggtccc tataatatgt gtagcttaac tttaagatag aactaagtca 240acaagatccg gagacaccat gtcaaagttc tatgttctta tgattagatg aacaaagtta 300tgatttattc aagattatca cgattcgaac tagttctaaa cttgtaaacg taaaataata 360aataacgttt gcaactacaa cactgaacat aaattaatga agtttgcttg atgtgttggt 420taaaatttgg aatgggacaa taaaaagaag aagagaactg agagaagtgg tgggggaagt 480gggaacctta acttccccac gtgacactaa cgagcaacgt taattgaata gtagtaggcc 540ctttttgtag tacactcttt tatatatggt caccttttaa ttagtttgca aatcacatac 600ttttaaaatc ataaaagcaa ttaacgttgc tcgttggtgt catatgcgat tgtagttagt 660ataatcgatc ttaattaaaa caaagtccat attaaaaatg accaaagtat gtaaaaataa 720aatgttgaat tagcatatgg tcactgatct tttctatttt aagatttagt aaacctaaga 780tgtatccgca agcctatagg gtttgatgtg gattactact agttactttt gtatgggctt 840gaagaaactt cattgggcca caaagaggga ggagtgagcg aagagcccaa attcaaacaa 900aaggatatga gggttcaaat gttattataa aatatattgt ggccaatttg taaagaaact 960tgataattac tttaaaagcc ccaacacaat tatctttaca 1000109998DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0385 as found in Promoter Report #146 109actcaacaat aggacaagcc aaaaaaattc caattattgt gttactctat tcttctaaat 60ttgaacacta atagactatg acatatgagt atataatgtg aagtcttaag atattttcat 120gtgggagatg aataggccaa gttggagtct gcaaacaaga agctcttgag ccacgacata 180agccaagttg atgaccgtaa ttaatgaaac taaatgtgtg tggttatata ttagggaccc 240atggccatat acacaatttt tgtttctgtc gatagcatgc gtttatatat atttctaaaa 300aaactaacat atttactgga tttgagttcg aatattgaca ctaatataaa ctacgtacca 360aactacatat gtttatctat atttgattga tcgaagaatt ctgaactgtt ttagaaaatt 420tcaatacact taacttcatc ttacaacggt aaaagaaatc accactagac aaacaatgcc 480tcataatgtc tcgaaccctc aaactcaaga gtatacattt tactagatta gagaatttga 540tatcctcaag ttgccaaaga attggaagct tttgttacca aacttagaaa cagaagaagc 600cacaaaaaaa gacaaaggga gttaaagatt gaagtgatgc atttgtctaa gtgtgaaagg 660tctcaagtct caactttgaa ccataataac attactcaca ctcccttttt ttttcttttt 720ttttcccaaa gtaccctttt taattccctc tataacccac tcactccatt ccctctttct 780gtcactgatt caacacgtgg ccacactgat gggatccacc tttcctctta cccacctccc 840ggtttatata aacccttcac aacacttcat cgctctcaaa ccaactctct cttctctctt 900ctctcctctc ttctacaaga agaaaaaaaa cagagccttt acacatctca aaatcgaact 960tactttaacc accaaatact gattgaacac acttgaaa 9981101000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0371 as found in Promoter Report #147 110gatatatttg tttaataatg cctacgattc tgcgaagaca ggagaagcca tacctttcaa 60tctaagccgt caacttgttc ccttacgtgg gatcctatta tacaatccaa cggttctaaa 120tgagccacgc cttccagatc taacacagtc atgccttcta cagtctgcac cccttttttt 180tttagtgttt tatctacatt ttttcctttg tgtttaattt tgtgccaaca tctataactt 240acccctataa aaatattcaa ttatcacaga atacccacaa tcgaaaacaa aatttaccgg 300aataatttaa ttaaagctgg actataatga caattccgaa actatcaagg aataaattaa 360agaaactaaa aaactaaagg gcattagagt aaagaagcgg caacatcaga attaaaaaac 420tgccgaaaaa ccaacctagt agccgtttat atgacaacac gtacgcaaag tctcggtaat 480gactcatcag ttttcatgtg caaacatatt acccccatga aataaaaaag cagagaagcg 540atcaaaaaaa tcttcattaa aagaacccta aatctctcat atccgccgcc gtctttgcct 600cattttcaac accggtgatg acgtgtaaat agatctggtt ttcacggttc tcactactct 660ctgtgatttt tcagactatt gaatcgttag gaccaaaaca agtacaaaga aactgcagaa 720gaaaagattt gagagagata tcttacgaaa caaggtatat atttctcttg ttaaatcttt 780gaaaatactt tcaaagtttc ggttggattc tcgaataagt taggttaaat agtcaatata 840gaattataga taaatcgata ccttttgttt gttatcattc aatttttatt gttgttacga 900ttagtaacaa cgttttagat cttgatctat atattaataa tactaatact ttgttttttt 960ttgttttttt tttaatacat attttgcttt tggaagatca 10001111000DNAArabidopsis thalianamisc_featureCeres Promoter construct PT0610 as found in Promoter Report #148 111ttagtgaaat tatgacatta agtaaggttt tcttagttag ctaatgtatg gctattcaat 60tgttatgtta ggctatttta gttagtatat gaatttaggc agtctatgca aatgatttcg 120ttttcatttt ttcatatgta aacatcaaga tcaagtaacg ccattcgagt tgatattttt 180tttttaaatt agtgtgtgta aattttggac cgcttatttg agtttgctaa tgaagttgca 240tatatattac gttaaaccat aggcaaacta atttgaaaca tccgattcga tttcctgtaa 300tttttcttgg ttaattgacc aaaatcaaga tcttcagaaa taaaataaaa gacgaaagaa 360agctgtcgca aagcagattg tgttaaaaaa aagtggattg ggctcaaacg caacttgtcc 420agcccgtgac aattacccta tacgcaagta agagtaacgt atcactggca aaagttggta 480ttagttacga tatctttgtc atgggggcat gcatgggcat ggcttaagag ttaagcctta 540agaagagtcc cacactcgtg actctcatga tcacttgttg tttcttacgg gcaaatacat 600ttaactttat tcttcattta ttcacctata ttcttttgga taataacttt tctctatata 660aaataacaaa catcgtacgt ttcatttatt tacaacaagc gatgagaatt aaaaggagac 720cttaattgat gatactcttc ttttctctcg gttacaacgg gattattaca gataatgata 780atctatatgg atgctgacgt ggaaaaacaa aatttggtga aacacgtcaa ttaagcacga 840cttttccatg gctagtggct aagatcgttt catcacatgg ctatatcata taatacttgg 900atgaattcaa aataaacgac tgagaaaatg tccacgtcac ggcgcaccgc tttggactta 960agtctcctat aataaataca acaccaaaca ttgcattcca 10001121000DNAArabidopsis thalianamisc_featureCeres Promoter construct PT0590 as found in Promoter Report #152 112attattcaat ttaataaaaa ttgagtcggc caatttaatg cgagacttct gtacaacgac 60cctaaaagtg ggtttgataa atgaaacata ttgcaacaaa aaaatactag taataatgat 120aaaatagtaa catgtcatgg cgcattgaat atcctacgaa ggtttagtgt ttacttttaa 180aaaatcctaa tatgatacta gtacatatag ctagcttgcc ttgcttatgc tattgcatag 240tctgtattaa taaatgatgt tatacatttc gatagagtaa cattttggga acatgagtga 300acgtgcttga atcttcgtgc ccttgacgtc agaagctagt aattttaaat actaattaac 360attcatacaa attaacagat acaatgtact atatcataat tcgtttccgt aacacaacgc 420aacaatttga aagtagatgt actttagtac ttagttagtg tgcaccaaaa aaaaaagatg 480tagttagtta gtaaggggtt aaatgtttta atttattaag aaaacttaaa ttcattaaat 540gttagaaaaa gtctaattag tttatattcg aacactgtgc tcaaaattaa aaagtcaact 600attttagact atagagttta ttaattaata ataaattcga taaatcaccg tattattttc 660ttcaacgaca agtagccgtg aagacacggg agcgaagaga gataaacaga agatgaagaa 720gaagatcaat gtcataatct tcagggagat aaatccgtaa tctttattaa tcaaggttaa 780tccttttttt tttcttcatc ttaattcttt gcgtcttcct tttctattta tcacgagatc 840tgtctttctt tttcctcttc tttctctctc ttctctctga agacagtact tgtttctgtc 900cggcgttaaa agcttcggtg gtggtctctt gacttctctg agaagaagaa aaggaagctg 960agtctcattt tagattcagc tcacgaggaa gtgacgacga 10001131000DNAArabidopsis thalianamisc_featureCeres Promoter construct PT0629 as found in Promoter Report #154 113tttaagcata tcaaatgtga aaattggtct taggttatga tgtatatcct tatatagtaa 60aagtatatta gtctaaaaat ttagatttaa aaagtgaact aaaatttaaa agcaagctga 120attacataaa tcaagtgaca attgtaattt gttgtgacat ttgtaagttc ataatggtac 180gtgtcacgct atgttaatta agtttgaaat atgagatgta agaaaaaagt gatcaaaaat 240taagttcttt tggagcgtta agtccaaagg tcaattagag cgcaatatca gtccagaaaa 300gtggatgaaa aatgtccatc catccatcga gaaatatgct taaagtaaac gcttactttt 360caagcgacct tcacggtatt gccgcagtca aaaatacgcg gcgactgttt ggttggtata 420gtgacgaccc actctcacgc ggattcatta atttcctatc acaaataaga aaaaagaaac 480gtgaaattaa tttgttaagt aacaaaaaca aaagaaaaag gaaactgaca agaaacgcta 540aagtggttcc ttccttgtac tcaaaaaaac gttaatttac agctcagaat aagtttttct 600gtttaccgta atttcgaccc aatattttta cgaataacaa tttagtccaa aacctctttg 660atatattgca atttaatacc ctaattaagt cttcttatct agtaatttgg ttttatttcc 720atgacctaca atataagata atagtcatta gtgctagata taaagactga attagggtat 780ataagatata tggaatgcta agattacaaa gagatagaga atcaaaaaaa ggaaagaatt 840aaggggcaaa tatgaaataa ttaaaaacaa gggagccaaa ggaacaatgt aagaaaagca 900ataaaattaa aaacaaaacc ctcaaaaaga agaaagcgga gactcgttag ctttttcctg 960cctcctataa attgaacgcg acttgcagaa cctttcctca 10001141000DNAArabidopsis thalianamisc_featureCeres Promoter construct PT0642 as found in Promoter Report #160 114ttgaacatcc ttaatttgaa ccataaaaaa tatgacatta actatctgat taattttcac 60ttaagggatg gttagttaag ttacattgga ttaaaaatgg tattagtaga ccaattagaa 120catgtatgcc attttttgtt tacaaaaacc tttttaagtg gtatttataa gacttgctca 180atttcattca aaagaacatg aaatggatgg actagtttta atggcaataa cccacacaat 240tactcatatt tggttcaaca aacttctatt tcggttcact tattaatttt ccatctatat 300atgaatcata taatatgatt aatttacaca agttcacacg gccacgtaaa atgtaactgt 360cttcaagttg tgcacataaa gagggtagtt tcgaagataa cgggtcaacg aaagggtaaa 420agagtaaatt gcatagaacg cggccaaatt aaaagccccc aattgggata aaagtcatcg 480ccgtctctta ggtgtcaaat ctcaactgtc taaaaacatt aaaagcttcg ttggggtagt 540tgcatcttcc ctctctaata caaaacttat tttatccagt tttagtttcg atttttcatt 600tgttaacatg ttttcatttt tcttttaatg ttaatagcct ttttatttgg aaaatgaaaa 660caatttcaca atttaattct aaattaccat ttctaaaaaa tagaaataat ataaataata 720atattgaata atacatggac taaaaaatta tagtactgtc ctaaacaaaa ttgcttgact 780agattgaaca gaaaatgttt ttgatgtctt actagtttga acaatttatt tgctaacatt 840attctctttg tatatttctt aaaaacccat attttttcct taaatatttc ccatttcccc 900taactacatt caatagctaa gtctctctct ccctctctct ttctctctca ctcaaaaatt 960tcccattaaa ttctcaaatt ttctccaact ttttaggcca 10001151000DNAArabidopsis thalianamisc_featureCeres Promoter construct PT0623 as found in Promoter Report #161 115aaagttattg acattttgaa aggaccgtaa atattaccaa aaaactgacg gagttaggat 60cggccacgta gaaagggaca aagagagaac agtcacggac tcggccagac taagtatggg 120cctgtctgaa tccaaactca gctaagttcc aaaagcataa agagagatgt gtaatgaaat 180gaacgtattc tagaaacgaa agcaatgtta tgctttgttt ttgagccaca tgtttttggg 240agatggagag aatctttttt acgtttttaa cctaacccac ttggcacttg gccaaaaaag 300tgagaagaaa ctgtggcgaa tgagtaggcc acgccatgga ctttgttcct tgtccttcaa 360aagttaaatt tatgttatgc gtggggacaa tctaagcaac gtggttcctt taaatatcgc 420agcttcctct tttacacttt tggagcctac gtgttttgtt ttggaccggc caaatacacg 480agtcagtcag tttagaaata atttggatgt ccaaaaatct tggagatcca aataaaataa 540ttagcatgtt ttagttcata agaatatgaa atgtagataa actgtctata ttaatttttc 600catagaattg gctttttatc gaggtgatgt acttaatgac tttgttgatt actactcgta 660taacaataaa gaatatgata ctatgtgaga cttataatga atttggtgtg tgttaattaa 720tccagttgaa acagtttaat aacaaatcag aataaaaatt gtagtaagaa aatttgaacg 780ctgatccttc aacctagata gtgaaccttt caaatactat atgattcacg tgtaatgttt 840ttgaccgttg gttatttttg tgtgaactat attaacttat caatatcgaa aggctaaata 900agtaaataac taaaagaaag ttcaggaaac aactcgacct aatgacctat catttctgat 960cacccgtcct ataaatacat acgtaagatc attcgttact 10001161000DNAArabidopsis thalianamisc_featureCeres Promoter construct PT0613 as found in Promoter Report #163 116ttaatactaa cattgtagaa agccacaaaa aagaaattga aatgtgagta gatgctgagt 60cagaggtttg gtcaatacac aacagctaat tgagataata ttatacacgt cacgatgact 120tgttttttct cctcccaact tgttaatttc tttattctta aaattaaacc atcgcaaaaa 180cagaagaaca cagctgtttt tctcgactcc caatttctat tttgctgcta aggacatttc 240atttcattat ttcccaattc aggactcctt agattttcct aaatttgttt tcctaacttg 300ctctctctca ttctaacatt ttctcatttt tttagattat cttgtacttt ttagtagatt 360attttatcag gttttacaaa catacattga cattctaaaa agggcttcta aaaattcagt 420gtggaatgct gatatactaa aaaaaggtca tgcaaaatta tctacgattt atctaaaatt 480agataatttg ccatatataa ctattaacta ataatcgatc ctttgatttt ttgtttagat 540aaaacgaaac agctatatct tttttttttg ttatcggatt ttaatcgaat aaaagctgaa 600aaataacagt tatatcttct tcttttttaa ctaatgaaac agttatatct taaacaaaca 660acagaaacag taaaatatta atgcaaatcc gcgtcaagag ataaatttta acaaactaat 720aacaattgag ataagattag cgcaaaagaa actctaattt tagagcgtgt aaacacaaac 780acgtcttgaa agtaaacgtg aattacacgc ttctaaaacg agcgtgagtt ttggttataa 840cgaagatacg gtgaagtgtg acacctttct acgttaattt cagtttgagg acacaactca 900agttatgttt gatatctaag gacttgcact gtctccaaat ctgcaggaag gactttttga 960ttggatcaat ataaatacca tctccattct cgtctccttc 10001171000DNAArabidopsis thalianamisc_featureCeres Promoter construct YP0093 as found in Promoter Report #261 117atgatgaaca ttctacatat ataattatta tgtttaagca cttagacagc ataaattctt 60tctaattata taaatctaac cttgttacat tgtacatcta taaattactt gaagaaataa 120cgagttctat ttctttttaa aaattaaaaa tactatacca tatctcagtg attaagttga 180accaaaaggt acggaggaga aacaagcatt tgattcttcc ttattttatt ttattcatct 240ctcactaatg atggtggaga aaaaaagaaa atacctaaca aacaaatata tattgtcata 300caaaaatatt tctatatttt tagttaatta gtttatattc ctcacttttc agggcttata 360taagaaagtg agcaaacaca aatcaaaatg cagcagcaaa tactatcatc acccatctcc 420ttagttctat tttataattc ctcttctttt tgttcatagc tttgtaatta tagtcttatt 480tctctttaag gctcaataag aggaggtact attactacac ttctctctac ttttacttgt 540attttagcat taaaatccta aaatccgttt taaattcaaa aataaactta gagatgttta 600atctcgattc ggtttttcgg ctttaggaga ataattatat gaaattagta tggatatctt 660tactagtttc cattcaaatg attctgattt caatctaata ctctcactct ttaattaaac 720tatatgtagt gtaatttcac actgttaaat ttctaccatg tcatgtatat tagagttgca 780tagaaaattg taaaacatcc atttgaattc gaatgaaaca aaatgtttta aaataaaatt 840ttggttttta aaagaaaaat ctaaaactga attatatcgt ttaaccaagt tgtaaaagtc 900ataaaacgta gtatcttgta aatcgctctt ccacggtcca aatagacttc tagtaataaa 960caagtaaaac taattttggt ttcttactaa ttttcacaga 1000

Claims

1. An isolated nucleic acid molecule capable of modulating transcription wherein the nucleic acid molecule shows at least 80% sequence identity to a sequence set forth in the Sequence Listing or a complement thereof.

2. The isolated nucleic acid molecule of claim 1, wherein said nucleic acid is capable of functioning as a promoter.

3. The isolated nucleic acid molecule of claim 1, wherein said nucleic acid molecule is capable of modulating transcription during particular developmental times or in response to a stimuli or in a particular cell, tissue, or organ as set forth in the Sequence Listing-Miscellaneous Feature.

4. A vector construct comprising:a) a first nucleic acid capable of modulating transcription wherein the nucleic acid molecule shows at least 80% sequence identity to a sequence set forth in the Sequence Listing; andb) a second nucleic acid having to be transcribed,wherein said first and second nucleic acid molecules are heterologous to each other and are operably linked together.

5. A host cell comprising an isolated nucleic acid molecule according to claim 1, wherein said nucleic acid molecule is flanked by exogenous sequence.

6. A host cell comprising a vector construct of claim 4.

7. A method of modulating transcription by combining, in an environment suitable for transcription:a) a first nucleic acid molecule capable of modulating transcription wherein the nucleic acid molecule shows at least 80% sequence identity to a sequence set forth in the Sequence Listing; andb) a second molecule to be transcribed;wherein the first and second nucleic acid molecules are heterologous to each other and operably linked together.

8. The method according to any one of claims 7, wherein said first nucleic acid molecule is capable of modulating transcription during the particular developmental times or in response to a stimuli or in a particular cell tissue, or organ as set forth in the Sequence Listing-Miscellaneous Feature wherein said first nucleic acid molecule is inserted into a plant cell and said plant cell is regenerated into a plant.

9. A plant comprising a vector construct according to claim 4.

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