COMPOSITIONS AND METHODS FOR PRODUCING PLANTS WITH IMPROVED STRESS TOLERANCE

Abstract

mpositions and methods useful for producing plant cells or plants with altered tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

Description

REFERENCE TO RELATED APPLICATIONS

[0001]The present application is a divisional of U.S. patent application Ser. No. 11/584,820, filed on Oct. 20, 2006, which claims priority under 35 U.S.C. §119(e) to U.S. Provisional Application No. 60/729,316, filed Oct. 21, 2005, each of which is hereby incorporated by reference in its entirety.

TECHNICAL FIELD

[0002]The present invention relates to compositions and methods for producing plants with improved stress tolerance.

BACKGROUND ART

[0003]Environmental abiotic stresses, including drought stress, cold stress, freezing stress, heat stress and salinity stress are major factors limiting plant growth and productivity. Crop losses and reduction in yield of major crops including maize, wheat and rice caused by such stresses represent significant economic issues and also lead to food shortages in several underdeveloped countries.

[0004]The development of stress tolerant plants has the potential to reduce or solve at least some of these problems. The use of traditional plant breeding strategies to produce new lines of plants that exhibit tolerance to these types of stresses has been slow. Lack of sufficient germplasm resources and incompatibility between distantly related plant species, present significant problems in conventional breeding. Further, the cellular processes leading to tolerance to such stresses are complex and involve multiple mechanisms of cellular adaptation and numerous metabolic pathways. This limits the success of both traditional breeding and that of genetic engineering approaches to development of stress tolerant plants. It would be beneficial to identify genes and proteins involved in controlling the complex processes leading to stress tolerance.

[0005]Regulators of gene expression, such as transcription factors, involved in controlling stress tolerance may be particularly useful in genetic engineering of plants, as a single gene may control a whole cascade of genes leading to the tolerance phenotype. Furthermore, there is sometimes commonality in many aspects of the different types of stress tolerant responses referred above. For example, genes that increase tolerance to cold or salt may also improve drought stress tolerance. This has been demonstrated in the case of the transcription factor At CBF/DREB 1 (Kasuga et al., 1999 Nature Biotech 17: 287-91) and the vacuolar pyrophosphatase AVP1 (Gaxiola et al., 2001 PNAS 98:11444-19).

[0006]Whilst some potentially useful genes have been identified, the identification and cloning of plant genes that confer tolerance to stress remains fragmented and incomplete. Although it is assumed that stress induced proteins may have a role in stress tolerance, evidence is still lacking and the function of many such stress responsive genes is unknown.

[0007]The hot and dry weather conditions in New Zealand and other countries in the summer period can have significant effect upon the yield of ryegrass. This is invariably during the dairy milking season and therefore has real effects on cost of dairy production through either reduced milk yield or the use of supplementary feeds and/or irrigation systems.

[0008]It would be beneficial to identify genes which have the capacity to confer stress tolerance in stress susceptible plant species. The development of stress tolerant crops will provide many advantages such as increasing yield and producing plants that may be cultivated in previously unsuitable environmental conditions. Thus, there exists a need for compositions and methods for producing plants with improved stress tolerance relative to their wild-type counterparts.

[0009]It is an object of the invention to provide improved compositions and methods for developing plant varieties with improved tolerance of at least one of the following stresses; drought, cold, freezing, heat and salinity, or at least to provide the public with a useful choice.

SUMMARY OF THE INVENTION

[0010]In the first aspect, the invention provides an isolated polynucleotide comprising:

[0011]a) a sequence encoding a polypeptide with at least 70% identity to the amino acid sequence of SEQ ID NO:4 or SEQ ID NO:163, wherein the polypeptide is capable of modulating in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity; or

[0012]b) the complement of the sequence of a).

[0013]Preferably the polypeptide has at least 70% identity to the amino acid sequence of SEQ ID NO:4. More preferably the polypeptide has the amino acid sequence of SEQ ID NO:4.

[0014]Preferably the sequence encoding the polypeptide in a) has at least 70% identity to the sequence of SEQ ID NO:82. More preferably the sequence encoding the polypeptide in a) has at least 70% identity to the coding sequence of SEQ ID NO:82. More preferably the sequence encoding the polypeptide in a) has the sequence of SEQ ID NO:82. More preferably the sequence encoding the polypeptide in a) has the coding sequence of SEQ ID NO:82.

[0015]In one embodiment the polypeptide is derived from a plant species and comprises the sequence of SEQ ID NO:1.

[0016]In a further embodiment the polypeptide is derived from a dicotyledonous species and comprises the sequence of SEQ ID NO:2.

[0017]In a further embodiment the polypeptide is derived from a monocotyledonous species and comprises the sequence of SEQ ID NO:3.

[0018]Alternatively the polypeptide has at least 70% identity to the amino acid sequence of SEQ ID NO:163. Preferably the polypeptide has the amino acid sequence of SEQ ID NO:163.

[0019]Preferably the sequence encoding the polypeptide in a) has at least 70% identity to the sequence of SEQ ID NO:201. More preferably the sequence encoding the polypeptide in a) has at least 70% identity to the coding sequence of SEQ ID NO:201. More preferably the sequence encoding the polypeptide in a) has the sequence of SEQ ID NO:201. More preferably the sequence encoding the polypeptide in a) has the coding sequence of SEQ ID NO:201.

[0020]In one embodiment the polypeptide is derived from a plant species and comprises the sequence of SEQ ID NO:4.

[0021]In a further embodiment the polypeptide is derived from a dicotyledonous species and comprises the sequence of SEQ ID NO:5.

[0022]In a further embodiment the polypeptide is derived from a monocotyledonous species and comprises the sequence of SEQ ID NO:6.

[0023]In a further embodiment the polypeptide comprises at least two repeats of the amino acid sequence motif QALGGHK (SEQ ID NO:242), the repeated moiety being separated by between 26 and 63 residues.

[0024]In a further aspect the invention provides an isolated polynucleotide comprising:

[0025]a) a sequence with at least 70% identity to the nucleotide sequence of SEQ ID NO:82 or 201, wherein the sequence encodes a polypeptide capable of modulating in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity; or

[0026]b) the complement of the sequence of a).

[0027]Preferably the sequence in a) has at least 70% identity to the sequence of SEQ ID NO:82. More preferably the sequence in a) has at least 70% identity to the coding sequence of SEQ ID NO:82. More preferably the sequence in a) has the sequence of SEQ ID NO:82. More preferably the sequence in a) has the coding sequence of SEQ ID NO:82.

[0028]Alternatively the sequence in a) has at least 70% identity to the sequence of SEQ ID NO:201. More preferably the sequence in a) has at least 70% identity to the coding sequence of SEQ ID NO:201. More preferably the sequence in a) has the sequence of SEQ ID NO:201. More preferably the sequence in a) has the coding sequence of SEQ ID NO:201.

[0029]In a further aspect the invention provides an isolated polypeptide comprising: [0030]a) a sequence with at least 70% identity to the amino acid sequence of SEQ ID NO:4 or 163, wherein the polypeptide is capable of modulating in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0031]Preferably the sequence in a) has at least 70% sequence identity to the amino acid sequence of SEQ ID NO: 4. More preferably the sequence in a) has the sequence of SEQ ID NO: 4.

[0032]Alternatively the sequence in a) has at least 70% sequence identity to the amino acid sequence of SEQ ID NO: 163. Preferably the sequence in a) has the sequence of SEQ ID NO: 163.

[0033]In a further aspect the invention provides an isolated polypeptide comprising a fragment of at least 5 contiguous amino acids of the polypeptide of the invention, wherein the fragment has essentially the same activity as the polypeptide.

[0034]In a further aspect the invention provides a polynucleotide encoding a polypeptide of the invention.

[0035]In a further aspect the invention provides an antibody raised against a polypeptide of the invention.

[0036]In a further aspect the invention provides a probe or primer capable of hybridizing to a polynucleotide of the invention, or a complement thereof, under stringent hybridization conditions.

[0037]Preferably the probe or primer comprises at least 15 contiguous nucleotides of polynucleotide of the invention.

[0038]In a further aspect the invention provides a genetic construct comprising a polynucleotide of the invention.

[0039]Preferably the genetic construct comprises a promoter operably linked to the polynucleotide. Preferably the promoter is the double CaMV 35S promoter. More preferably the promoter is the ryegrass promoter of SEQ ID NO:239. Alternatively the promoter is a portion of the ryegrass promoter of SEQ ID NO:239 that provides essentially the same expression pattern as the ryegrass promoter of SEQ ID NO:239.

[0040]In a further aspect the invention provides a vector comprising the genetic construct of the invention.

[0041]In a further aspect the invention provides a host cell comprising a genetic construct of the invention.

[0042]In a further aspect the invention provides a host cell genetically modified to express the polynucleotide of the invention.

[0043]In a further aspect the invention provides a plant cell comprising the genetic construct of the invention.

[0044]In a further aspect the invention provides a plant cell genetically modified to express a polynucleotide of the invention.

[0045]In a further aspect the invention provides a plant which comprises the plant cell of the invention.

[0046]In a further aspect the invention provides a method for producing a polypeptide of the invention, the method comprising culturing a host cell comprising a genetic construct of the invention designed to express the polypeptide.

[0047]In a further aspect the invention provides a method for producing a plant cell or plant with altered tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity; the method comprising the step of transformation of a plant cell or plant with a genetic construct including:

[0048]a) at least one polynucleotide of the invention; or

[0049]b) at least one polynucleotide comprising a fragment, of at least 15 nucleotides in length, of the polynucleotide of a);

[0050]In a further aspect the invention provides a plant produced by the method of the invention.

[0051]In a further aspect the invention provides a method for selecting a plant with altered tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity, the method comprising testing of a plant for altered expression of a polynucleotide of the invention.

[0052]In a further aspect the invention provides a method for selecting a plant with altered tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity, the method comprising testing of a plant for altered expression of a polypeptide of the invention.

[0053]In a further aspect the invention provides a plant selected by a selection method of the invention.

[0054]In a further aspect the invention provides a plant part, propagule, progeny or seed of the plant of the invention.

[0055]In preferred embodiments of the invention the environmental stress is drought stress.

[0056]In a further aspect the invention provides an isolated polynucleotide comprising the sequence of SEQ ID NO:82 or a variant thereof, wherein the variant encodes a polypeptide which modulates in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0057]Preferably the variant of SEQ ID NO:82 encodes a polypeptide comprising the amino acid sequence:

TABLE-US-00001 (SEQ ID NO: 1) LX₁X₂X₃X₄X₅X₆LX₇X₈X₉KX₁₀- X₁₁X₁₂RX₁₃EKX₁₄X₁₅X₁₆K wherein X₁ = Q, E, H, L, R, T or S, X₂ = E, D or Q, X₃ = K, R, A, E or T, X₄ = I or S, X₅ = K, R, D or N, X₆ = E, D or S, X₇ = K, T or I, X₈ = A, V, S, T, D, V or Q, X₉ = E or D, X₁₀ = N, D, T or S, X₁₁ = E or D, X₁₂ = L or S, X₁₃ = D, H or E, X₁₄ = Q, H, R, L, V, T or A and X₁₅ = K, R, V, T or S, X₁₆ = L or M,

the polypeptide modulating, in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0058]Alternatively the variant of SEQ ID NO:82 encodes a polypeptide comprising the amino acid sequence:

TABLE-US-00002 (SEQ ID NO: 2) LX₁X₂X₃X₄X₅X₆L.sub.X7X₈X₉KX₁₀- X₁₁LRX₁₂EKX₁₃X₁₄X₁₅K wherein X₁ = Q, H, L, R, T or S, X₂ = E, D or Q, X₃ = K, R, A or E, X₄ = I or S, X₅ = K, R, D or N, X₆ = E, D or S, X₇ = K, T or I, X₈ = A, V, S, T, D, or Q, X₉ = E or D, X₁₀ = N, T or S, X₁₁ = E or D, X₁₂ = D or E, X₁₃ = Q, H, R, L, T or A, X₁₄ = K, R, V, T or S and X₁₅ = L or M,

the polypeptide modulating, in a dicotyledonous plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0059]Alternatively the variant of SEQ ID NO:82 encodes a polypeptide comprising the amino acid sequence:

TABLE-US-00003 (SEQ ID NO: 3) LX₁X₂X₃IX₄X₅LKX₆X₇KX₈EX₉RX.su- b.10EKX₁₁X₁₂X₁₃K where X₁ = Q, E or R, X₂ = E or D, X₃ = K or T, X₄ = K or R, X₅ = E or D, X₆ = A or V, X₇ = E or D, X₈ = N or D, X₉ = L or S, X₁₀ = D or H, X₁₁ = Q or V, X₁₂ = K, R or T and X₁₃ = L or M,

the polypeptide modulating, in a monocotyledonous plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0060]Exemplary polynucleotide variants of SEQ ID NO: 82 are disclosed herein and identified as SEQ ID NOs: 83-159 of the sequence listing.

[0061]In a further aspect the invention provides an isolated polynucleotide comprising the sequence of SEQ ID NO:82.

[0062]In a further aspect the invention provides an isolated polynucleotide consisting of the sequence of SEQ ID NO:82.

[0063]In a further aspect the invention provides polynucleotides comprising fragments of SEQ ID NO: 82. Polynucleotides comprising fragments of the polynucleotide variants also form part of the invention.

[0064]In a further aspect the invention provides an isolated polynucleotide comprising the sequence of SEQ ID NO: 201 or a variant thereof, wherein the variant encodes a polypeptide which modulates in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0065]Preferably the variant of SEQ ID NO:201 encodes a polypeptide comprising the amino acid sequence:

TABLE-US-00004 (SEQ ID NO: 160) X₁X₂CX₃VCX₄X₅X₆X₇X₈X₉YQALGGHK- X₁₀SHRX₁₁ where X₁ = H, F or Y, X₂ = G, A, K, E or R, X₃ = S, T, N or G, X₄ = G, E, D, N or Y, X₅ = K or R, X₆ = A, S, V, G, or T, X₇ = F or Y, X₈ = A, P, S or G, X₉ = S or T, X₁₀ = A or T and X₁₁ = K, S, I, P, T or V

the polypeptide modulating, in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0066]Alternatively the variant of SEQ ID NO:201 encodes a polypeptide comprising the amino acid sequence:

TABLE-US-00005 (SEQ ID NO: 161) X₁X₂CX₃VCX₄KX₅FX₆SYQALGGHKX₇SHRX₈ where X₁ = H, F or Y, X₂ = G, A, K, E or R, X₃ = S, T, N or G, X₄ = G, E, D, N or Y, X₅ = A, S, G, or T, X₆ = A, P. S or G, X₇ = A or T and X₈ = K, S, I, P, T or V

the polypeptide modulating, in a dicotyledonous plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0067]Alternatively the variant of SEQ ID NO:201 encodes a polypeptide comprising the amino acid sequence:

TABLE-US-00006 (SEQ ID NO: 162) X₁X₂CSVCGX₃X₄X₅X₆SYQALGGHKX₇SHRX₈ where X₁ = H, F or Y, X₂ = G, A, K, E or R, X₃ = K or R, X₄ = A, S, V, or G, X₅ = F or Y, X₆ = A, P, S or G, X₇ = A or T, X₈ = K, P, T or V.

the polypeptide modulating, in a monocotyledonous plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0068]Alternatively the variant of SEQ ID NO:201 encodes a polypeptide comprising at least two repeats of the amino acid sequence motif QALGGHK (SEQ ID NO:244), the repeated sequence motif being separated by about 36 to about 60 residues, wherein the polypeptide modulates, in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity. Preferably the motif is separated by about 40 to about 60 residues, more preferably by about 50 to about 60 residues, most preferably by about 56 residues. Preferably the polypeptide encoded by the variant modulates tolerance to the environmental stress in a dicotyledonous plant. More preferably the polypeptide encoded by the variant modulates tolerance to the environmental stress in a moncotyledonous plant.

[0069]Exemplary polynucleotide variants of SEQ ID NO: 201 are disclosed herein and identified as SEQ ID NOs: 202-238 of the sequence listing.

[0070]In a further aspect the invention provides an isolated polynucleotide comprising the sequence of SEQ ID NO: 201.

[0071]In a further aspect the invention provides an isolated polynucleotide consisting of the sequence of SEQ ID NO: 201.

[0072]In a further aspect the invention provides polynucleotides comprising fragments of SEQ ID NO: 201. Polynucleotides comprising fragments of the polynucleotide variants also form part of the invention.

[0073]The isolated polynucleotides of the invention are also useful in methods for selecting plants tolerant to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0074]In a further aspect the invention provides an isolated polypeptide comprising the sequence of SEQ ID NO: 4 or a variant thereof, wherein the variant modulates in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0075]Preferably the variant of SEQ ID NO: 4 comprises the amino acid sequence:

TABLE-US-00007 (SEQ ID NO: 1) LX₁X₂X₃X₄X₅X₆LX₇X₈X₉KX₁₀- X₁₁X₁₂RX₁₃EKX₁₄X₁₅X₁₆K wherein X₁ = Q, E, H, L, R, T or S, X₂ = E, D or Q, X₃ = K, R, A, E or T, X₄ = I or S, X₅ = K, R, D or N, X₆ = E, D or S, X₇ = K, T or I, X₈ = A, V, S, T, D, V or Q, X₉ = E or D, X₁₀ = N, D, T or S, X₁₁ = E or D, X₁₂ = L or S, X₁₃ = D, H or E, X₁₄ = Q, H, R, L, V, T or A and X₁₅ = K, R, V, T or S, X₁₆ = L or M,

and modulates in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0076]Alternatively the variant of SEQ ID NO: 4 comprises the amino acid sequence:

TABLE-US-00008 (SEQ ID NO: 2) LX₁X₂X₃X₄X₅X₆L.sub.X7X₈X₉KX₁₀- X₁₁LRX₁₂EKX₁₃X₁₄X₁₅K wherein X₁ = Q, H, L, R, T or S, X₂ = E, D or Q, X₃ = K, R, A or E, X₄ = I or S, X₅ = K, R, D or N, X₆ = E, D or S, X₇ = K, T or I, X₈ = A, V, S, T, D, or Q, X₉ = E or D, X₁₀ = N, T or S, X₁₁ = E or D, X₁₂ = D or E, X₁₃ = Q, H, R, L, T or A, X₁₄ = K, R, V, T or S and X₁₅ = L or M,

and modulates in a dicotyledonous plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0077]Alternatively the variant of SEQ ID NO: 4 comprises the amino acid sequence:

TABLE-US-00009 (SEQ ID NO: 3) LX₁X₂X₃IX₄X₅LKX₆X₇KX₈EX₉RX.su- b.10EKX₁₁X₁₂X₁₃K where X₁ = Q, E or R, X₂ = E or D, X₃ = K or T, X₄ = K or R, X₅ = E or D, X₆ = A or V, X₇ = E or D, X₈ = N or D, X₉ = L or S, X₁₀ = D or H, X₁₁ = Q or V, X₁₂ = K, R or T and X₁₃ = L or M,

and modulates in a in a monocotyledonous plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0078]Exemplary polypeptide variants of SEQ ID NO: 4 are disclosed herein and identified as SEQ ID NOs: 5-81 of the sequence listing.

[0079]In a further aspect the invention provides an isolated polypeptide comprising the sequence of SEQ ID NO: 4.

[0080]In a further aspect the invention provides an isolated polypeptide consisting of the sequence of SEQ ID NO: 4.

[0081]In a further aspect the invention provides polypeptides comprising fragments of SEQ ID NO: 4. Polypeptides comprising fragments of variants, also form part of the invention.

[0082]In a further aspect the invention provides an isolated polypeptide comprising the sequence of SEQ ID NO: 163 or a variant thereof, wherein the variant modulates in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0083]Preferably the variant of SEQ ID NO: 163 comprises the amino acid sequence:

TABLE-US-00010 (SEQ ID NO: 160) X₁X₂CX₃VCX₄X₅X₆X₇X₈X₉YQALGGHK- X₁₀SHRX₁₁ where X₁ = H, F or Y, X₂ = G, A, K, E or R, X₃ = S, T, N or G, X₄ = G, E, D, N or Y, X₅ = K or R, X₆ = A, S, V, G, or T, X₇ = F or Y, X₈ = A, P, S or G, X₉ = S or T, X₁₀ = A or T and X₁₁ = K, S, I, P, T or V

and modulates in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0084]Alternatively the variant of SEQ ID NO: 163 comprises the amino acid sequence:

TABLE-US-00011 (SEQ ID NO: 161) X₁X₂CX₃VCX₄KX₅FX₆SYQALGGHKX₇SHRX₈ where X₁ = H, F or Y, X₂ = G, A, K, E or R, X₃ = S, T, N or G, X₄ = G, E, D, N or Y, X₅ = A, S, G, or T, X₆ = A, P. S or G, X₇ = A or T and X₈ = K, S, I, P, T or V

and modulates in a dicotyledonous plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0085]Alternatively the variant of SEQ ID NO: 163 comprises the amino acid sequence:

TABLE-US-00012 (SEQ ID NO: 162) X₁X₂CSVCGX₃X₄X₅X₆SYQALGGHKX₇SHRX₈ where X₁ = H, F or Y, X₂ = G, A, K, E or R, X₃ = K or R, X₄ = A, S, V, or G, X₅ = F or Y, X₆ = A, P, S or G, X₇ = A or T, X₈ = K, P, T or V.

and modulates in a monocotyledonous plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0086]Alternatively the variant of SEQ ID NO: 163 comprises at least two repeats of the amino acid sequence motif QALGGHK (SEQ ID NO:244), the repeated sequence motif being separated by about 36 to about 63 residues, wherein the polypeptide modulates, in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity. Preferably the motif is separated by about 40 to about 60 residues, more preferably by about 50 to about 60 residues, most preferably by about 56 residues. Preferably the polypeptide modulates tolerance to the environmental stress in a dicotyledonous plant. More preferably the polypeptide modulates tolerance to the environmental stress in a monocotyledonous plant.

[0087]Exemplary polypeptide variants of SEQ ID NO: 163 are disclosed herein and identified as SEQ ID NOs: 164-200 of the sequence listing.

[0088]In a further aspect the invention provides an isolated polypeptide comprising the sequence of SEQ ID NO: 163.

[0089]In a further aspect the invention provides an isolated polypeptide consisting of the sequence of SEQ ID NO: 163.

[0090]In a further aspect the invention provides polypeptides comprising fragments of SEQ ID NO: 163. Polypeptides comprising fragments of variants, also form part of the invention.

[0091]In a further aspect the invention provides a polynucleotide encoding a polypeptide of the invention.

[0092]In a further aspect the invention provides a genetic construct which comprises a polynucleotide of the invention.

[0093]In a further aspect the invention provides a genetic construct which comprises a polynucleotide encoding a polypeptide of the invention.

[0094]In a further aspect the invention provides a genetic construct which comprises a polynucleotide of any one of SEQ ID NOs: 82-159, or a variant or fragment thereof

[0095]In a further aspect the invention provides a genetic construct which comprises a polynucleotide of any one of SEQ ID NOs: 201-238, or a variant or fragment thereof.

[0096]In a further aspect the invention provides a genetic construct which comprises the polynucleotide sequence of SEQ ID NO: 82, or a variant or fragment thereof

[0097]In a further aspect the invention provides a genetic construct which comprises the polynucleotide sequence of SEQ ID NO: 82.

[0098]In a further aspect the invention provides a genetic construct which comprises the polynucleotide sequence of SEQ ID NO: 201, or a variant or fragment thereof

[0099]In a further aspect the invention provides a genetic construct which comprises the polynucleotide sequence of SEQ ID NO: 201.

[0100]Preferably the constructs of the invention are expression constructs as herein defined. Preferably expression constructs of the invention include an environmental stress responsive promoter operably linked polynucleotide sequence. Preferably the environmental stress responsive promoter is responsive to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0101]Preferably the expression construct includes a promoter comprising the sequence of SEQ ID NO: 239 or a fragment, region, cis-element or variant of the sequence capable of regulating transcription of an operably linked polynucleotide sequence.

[0102]In a further aspect the invention provides a vector which comprises a genetic construct of the invention.

[0103]In a further aspect the invention provides a host cell which comprises a genetic construct of the invention.

[0104]In a further aspect the invention provides methods for the recombinant production of polypeptide of the invention comprising the steps of: [0105]a) culturing a host cell comprising a genetic construct of the invention, such as an expression construct as defined herein, capable of expressing a polypeptide of the invention, and [0106]b) separating the expressed polypeptide.

[0107]In a further aspect the invention provides a plant cell which comprises one or more of the genetic constructs of the invention. In a preferred embodiment the genetic construct comprises the polynucleotide sequence of SEQ ID NO: 82 or a variant or fragment thereof.

[0108]In a further aspect the invention provides a plant cell which comprises one or more of the genetic constructs of the invention. In a preferred embodiment the genetic construct comprises the polynucleotide sequence of SEQ ID NOs: 201 or a variant or fragment thereof.

[0109]In a further aspect the invention provides a plant cell with altered expression of a polynucleotide or polypeptide of the invention.

[0110]In a further aspect the invention provides a plant cell genetically modified to alter expression of a polynucleotide or polypeptide of the invention.

[0111]In a further aspect the invention provides a plant which comprises a plant cell of the invention.

[0112]In a further aspect the invention provides methods for altering in a plant, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity, the method comprising transformation of a plant cell, or plant with a genetic construct of the invention capable of altering expression of a polynucleotide/polypeptide of the invention.

[0113]In a further aspect the invention provides methods for altering tolerance to drought stress in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide/polypeptide of the invention.

[0114]In a further aspect the invention provides methods for altering tolerance to cold stress in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide/polypeptide of the invention.

[0115]In a further aspect the invention provides methods for altering tolerance to freezing stress in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide/polypeptide of the invention.

[0116]In a further aspect the invention provides methods for altering tolerance to heat stress in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide/polypeptide of the invention.

[0117]In a further aspect the invention provides methods for altering tolerance to salinity stress in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide/polypeptide of the invention.

[0118]In a further aspect the invention provides methods for altering tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide involved in modulation in a plant of tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

[0119]In a further aspect the invention provides methods for altering tolerance to drought stress in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide involved in modulation tolerance to drought stress in a plant.

[0120]In a further aspect the invention provides methods for altering tolerance to cold stress in a plant the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide involved in modulation of tolerance to cold stress in a plant.

[0121]In a further aspect the invention provides methods for altering tolerance to freezing stress in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide involved in modulation of tolerance to freezing stress in a plant.

[0122]In a further aspect the invention provides methods for altering tolerance to heat stress in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide involved in modulation of tolerance to freezing stress in a plant.

[0123]In a further aspect the invention provides methods for altering tolerance to salinity stress in a plant, the method comprising transformation of a plant with a genetic construct of the invention capable of altering expression of a polynucleotide involved in modulation of tolerance to salinity stress in a plant.

[0124]It will be understood by those skilled in the art that transformation of a plant may involve transforming a plant cell/s and regenerating a transformed plant from the transformed plant cell/s.

[0125]In a further aspect the invention provides a method for selecting a plant with increased tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity, the method comprising testing of a plant for altered expression of a polynucleotide of the invention.

[0126]In a further aspect the invention provides a method for selecting a plant with increased tolerance to drought stress, the method comprising testing of a plant for altered expression of a polynucleotide of the invention.

[0127]In a further aspect the invention provides a method for selecting a plant with increased tolerance to cold stress, the method comprising testing of a plant for altered expression of a polynucleotide of the invention.

[0128]In a further aspect the invention provides a method for selecting a plant with increased tolerance to freezing stress, the method comprising testing of a plant for altered expression of a polynucleotide of the invention.

[0129]In a further aspect the invention provides a method for selecting a plant with increased tolerance to heat stress, the method comprising testing of a plant for altered expression of a polynucleotide of the invention.

[0130]In a further aspect the invention provides a method for selecting a plant with increased tolerance to salinity stress, the method comprising testing of a plant for altered expression of a polynucleotide of the invention.

[0131]In a further aspect the invention provides a method for selecting a plant with increased tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity, the method comprising testing of a plant for altered expression of a polypeptide of the invention.

[0132]In a further aspect the invention provides a method for selecting a plant with increased tolerance to drought stress, the method comprising testing of a plant for altered expression of a polypeptide of the invention.

[0133]In a further aspect the invention provides a method for selecting a plant with increased tolerance to cold stress, the method comprising testing of a plant for altered expression of a polypeptide of the invention.

[0134]In a further aspect the invention provides a method for selecting a plant with increased tolerance to freezing stress, the method comprising testing of a plant for altered expression of a polypeptide of the invention.

[0135]In a further aspect the invention provides a method for selecting a plant with increased tolerance to heat stress, the method comprising testing of a plant for altered expression of a polypeptide of the invention.

[0136]In a further aspect the invention provides a method for selecting a plant with increased tolerance to salinity stress, the method comprising testing of a plant for altered expression of a polypeptide of the invention.

[0137]In a further aspect the invention provides a plant cell or plant produced by a method of the invention.

[0138]The polynucleotides and polynucleotide variants, of the invention may be derived from any species and/or may be produced recombinantly or synthetically.

[0139]In one embodiment the polynucleotide or variant, is derived from a plant species.

[0140]In a further embodiment the polynucleotide or variant, is derived from a gymnosperm plant species.

[0141]In a further embodiment the polynucleotide or variant, is derived from an angiosperm plant species.

[0142]In a further embodiment the polynucleotide or variant, is derived from a from dicotyledonous plant species.

[0143]In a further embodiment the polynucleotide or variant, is derived from a monocotyledonous plant species.

[0144]The polypeptide and polypeptide variants, of the invention may be derived from any species and/or may be produced recombinantly or synthetically.

[0145]In one embodiment the polypeptide or variant, is derived from a plant species.

[0146]In a further embodiment the polypeptide or variant, is derived from a gymnosperm plant species.

[0147]In a further embodiment the polypeptide or variant, is derived from an angiosperm plant species.

[0148]In a further embodiment the polypeptide or variant, is derived from a from dicotyledonous plant species.

[0149]In a further embodiment the polypeptide or variant, is derived from a monocotyledonous plant species.

[0150]The plant cell or plant may be derived from any plant species.

[0151]In a further embodiment the plant cell or plant, is derived from a gymnosperm plant species.

[0152]In a further embodiment the plant cell or plant, is derived from an angiosperm plant species.

[0153]In a further embodiment the plant cell or plant, is derived from a from dicotyledonous plant species.

[0154]In a further embodiment the plant cell or plant, is derived from a monocotyledonous plant species.

[0155]Preferred dicotyledonous genera include: Amygdalus, Anacardium, Arachis, Brassica, Cajanus, Cannabis, Carthamus, Carya, Ceiba, Cicer, Cocos, Coriandrum, Coronilla, Crotalaria, Dolichos, Elaeis, lycine, Gossypium, Helianthus, Lathyrus, Lens, Lespedeza, Linum, Lotus, Lupinus, Macadamia, Medicago, Melilotus, Mucuna, Olea, Onobrychis, Ornithopus, Papaver, Phaseolus, Phoenix, Pistacia, Pisum, Prunus, Pueraria, Ribes, Ricinus, Sesamum, Theobroma, Trtfolium, Trigonella, Vicia and Vigna.

[0156]Preferred dicotyledonous species include: Amygdalus communis, Anacardium occidentals, Arachis hypogaea, Arachis hypogea, Brassica napus Rape, Brassica. nigra. Brassica campestris, Cajanus cajan, Cajanus indicus, Cannabis saliva, Carthamus tinctorius, Carya illinoinensis, Ceiba pentandra, Cicer arietinum, Cocos nucifera, Coriandrum sativum, Coronilla varia, Crotalaria juncea, Dolichos lablab, Elaeis guineensis, Gossypium arboreum, Gossypium narking, Gossypium barbadense, Gossypium herbaceum, Gossypium hirsutum, Glycine max, Glycine ussuriensis, Glycine gracilis, Helianthus annus, Lupinus angustifolius, Lupinus luteus, Lupinus mutabilis, Lespedeza sericea, Lespedeza striata, Lotus uliginosus, Lathyrus sativus, Lens culinaris, Lespedeza stipulacea, Linum usitatissimum, Lotus corniculatus, Lupinus albus, Medicago arborea, Medicago falcate, Medicago hispida, Medicago officinalis, Medicago. saliva Alfalfa, Medicago tribuloides, Macadamia integrifolia, Medicago arabica, Melilotus albus, Mucuna pruriens, Olea europaea, Onobrychis viciifolia, Ornithopus sativus, Phaseolus aureus, Prunus cerasifera, Prunus cerasus, Phaseolus coccineus, Prunus domestica, Phaseolus lunatus, Prunus. maheleb, Phaseolus mungo, Prunus. persica, Prunus. pseudocerasus, Phaseolus vulgaris, Papaver somniferum, Phaseolus acutifolius, Phoenix dactylifera, Pistacia vera, Pisum sativum, Prunus amygdalus, Prunus armeniaca, Pueraria thunbergiana, Ribes nigrum, Ribes rubrum, Ribes grossularia, Ricinus communis, Sesamum indicum, Trifolium augustifolium, Trifolium diffusum, Trifolium hybridum, Trifolium incarnatum, Trifolium ingrescens, Trifolium pratense, Trifolium repens, Trifolium resupinatum, Trifolium subterraneum, Theobroma cacao, Trifolium alexandrinum, Trigonella foenumgraecum, Vicia angustifolia, Vicia atropurpurea, Vicia calcarata, Vicia dasycarpa, Vicia ervilia, Vaccinium oxycoccos, Vicia pannonica, Vigna sesquipedalis, Vigna sinensis, Vicia villosa, Vicki faba, Vicia sative and Vigna angularis.

[0157]Preferred monocotyledonous genera include: Agropyron, Allium, Alopecurus, Andropogon, Arrhenatherum, Asparagus, Avena, Bambusa, Bothrichloa, Bouteloua, Bromus, Calamovilfa, Cenchrus, Chloris, Cymbopogon, Cynodon, Dactylis, Dichanthium, Digitaria, Eleusine, Eragrostis, Fagopyrum, Festuca, Helianthus, Hordeum, Lolium, Miscanthis, Miscanthus×giganteus, Oryza, Panicum, Paspalum, Pennisetum, Phalaris, Phleum, Poa, Saccharum, Secale, Setaria, Sorgahastum, Sorghum, Triticum, Vanilla, X Triticosecale Triticale and Zea.

[0158]Preferred monocotyledonous species include: Agropyron cristatum, Agropyron desertorum, Agropyron elongatum, Agropyron intermedium, Agropyron smithii, Agropyron spicatum, Agropyron trachycaulum, Agropyron trichophorum, Allium ascalonicum, Allium cepa, Allium chinense, Allium porrum, Allium schoenoprasum, Allium. fistulosum, Allium. sativum, Alopecurus pratensis, Andropogon gerardi, Andropogon Gerardii, Andropogon scoparious, Arrhenatherum elatius, Asparagus officinalis, Avena nuda, Avena sativa, Bambusa vulgaris, Bothrichloa barbinodis, Bothrichloa ischaemum, Bothrichloa saccharoides, Bouteloua curipendula, Bouteloua eriopoda, Bouteloua gracilis, Bromus erectus, Bromus inermis, Bromus riparius, Calamovilfa longifilia, Cenchrus ciliaris, Chloris gayana, Cymbopogon nardus, Cynodon dactylon, Dactylis glomerata, Dichanthium annulatum, Dichanthium aristatum, Dichanthium sericeum, Digitaria decumbens, Digitaria smutsii, Eleusine coracan, Elymus angustus, Elymus junceus, Eragrostis curvula, Eragrostis tef, Fagopyrum esculentum, Fagopyrum tataricum, Festuca arundinacea, Festuca ovina, Festuca pratensis, Festuca rubra, Helianthus annuus sunflower, Hordeum distichum, Hordeum vulgare, Lolium multiflorum, Lolium perenne, Miscanthis sinensis, Miscanthus×giganteus, Oryza sativa, Panicum italicium, Panicum maximum, Panicum miliaceum, Panicum purpurascens, Panicum virgatum, Panicum virgatum, Paspalum dilatatum, Paspalum notatum, Pennisetum clandestinum, Pennisetum glaucum, Pennisetum purpureum, Pennisetum spicatum, Phalaris arundinacea, Phleum bertolinii, Phleum pratense, Poa fendleriana, Poa pratensis, Poa. nemoralis, Saccharum officinarum, Saccharum robustum, Saccharum sinense, Saccharum spontaneum, Secale cereale, Setaria sphacelata, Sorgahastum nutans, Sorghastrum nutans, Sorghum dochna, Sorghum halepense, Sorghum sudanense, Sorghum bicolor, Triticum aestivum, Triticum dicoccum, Triticum durum, Triticum monococcum, Vanilla fragrans, X Triticosecale and Zea mays.

[0159]Preferred plants are forage plant species from a group comprising but not limited to the following genera: Lolium, Festuca, Dactylis, Bromus, Trifolium, Medicago, Phleum, Phalaris, Holcus, Lotus, Plantago and Cichorium.

[0160]Particularly preferred plants are from the genera Lolium and Trifolium. Particularly preferred species are Lolium perenne and Trifolium repens.

[0161]Particularly preferred monocotyledonous plant species are: Lolium perenne and Oryza sativa.

[0162]The term "plant" is intended to include a whole plant, any part of a plant, propagules and progeny of a plant.

[0163]The term `propagule` means any part of a plant that may be used in reproduction or propagation, either sexual or asexual, including seeds and cuttings.

BRIEF DESCRIPTION OF THE DRAWINGS

[0164]The present invention will be better understood with reference to the accompanying drawings in which:

[0165]FIG. 1 shows an alignment of polypeptides of the invention, including SEQ ID NO: 4 and sequences which are variants SEQ ID NO: 4 from several species and illustrates a consensus region (shown within bold box) identified by the applicants which is present in all of the such sequences.

[0166]FIG. 2 shows an alignment of polypeptides of the invention, including SEQ ID NO: 4 and sequences which are variants SEQ ID NO: 4 from several dicotyledonous species and illustrates a consensus region (shown within bold box) identified by the applicants which is present in all of the such sequences.

[0167]FIG. 3 shows an alignment of polypeptides of the invention, including SEQ ID NO: 4 and sequences which are variants SEQ ID NO: 4 from several monocotyledonous species and illustrates a consensus region (shown within bold box) identified by the applicants which is present in all of the such sequences.

[0168]FIG. 4 shows an alignment of polypeptides of the invention, including SEQ ID NO: 163 and sequences which are variants SEQ ID NO: 163 from several species and illustrates a consensus region (shown within bold box) identified by the applicants which is present in all of the such sequences.

[0169]FIG. 5 shows an alignment of polypeptides of the invention, including SEQ ID NO: 163 and sequences which are variants SEQ ID NO: 163 from several dicotyledonous species and illustrates a consensus region (shown within bold box) identified by the applicants which is present in all of the such sequences.

[0170]FIG. 6 shows an alignment of polypeptides of the invention, including SEQ ID NO: 163 and sequences which are variants SEQ ID NO: 163 from several monocotyledonous species and illustrates a consensus region (shown within bold box) identified by the applicants which is present in all of the such sequences.

[0171]FIG. 7 shows a map of a vector, for plant transformation, comprising ORF4 (SEQ ID NO:82) driven by the ryegrass promoter of SEQ ID NO:239.

[0172]FIG. 8 shows the sequence of a vector, for plant transformation, comprising ORF4 and corresponding to the map in FIG. 7. Sequence in bold corresponds to the Lolium perenne promoter (SEQ ID NO:239). Sequence in italics corresponds to ORF4. Sequence underlined corresponds to 3' terminator sequence from CaMV35S gene. Sequence in regular font corresponds to vector sequence.

[0173]FIG. 9 shows a map of a vector, for plant transformation, comprising ORF12 (SEQ ID NO:201) driven by the ryegrass promoter of SEQ ID NO:243

[0174]FIG. 10 shows the sequence of a vector, for plant transformation, comprising ORF12 and corresponding to the map in FIG. 9. Sequence in bold corresponds to the Lolium perenne promoter (SEQ ID NO:239). Sequence in italics corresponds to ORF12. Sequence underlined corresponds to the 3' terminator sequence from CaMV35S gene. Sequence in regular font corresponds to vector sequence.

[0175]FIG. 11 shows a map of a vector, for plant transformation, comprising ORF4 (SEQ ID NO:82) driven by the double CaMV35S promoter.

[0176]FIG. 12 shows the sequence of a vector, for plant transformation, comprising ORF4 (SEQ ID NO: 82) and corresponding to the map in FIG. 11. Sequence in bold corresponds to the double CaMV35S promoter. Sequence in italics corresponds to ORF4 (SEQ ID NO: 82). Sequence underlined corresponds to 3' terminator sequence from CaMV35S gene. Sequence in regular font corresponds to vector sequence.

[0177]FIG. 13 shows a map of a vector, for plant transformation, comprising ORF12 (SEQ ID NO:201) driven by the double CaMV35S promoter.

[0178]FIG. 14 shows the sequence of a vector, for plant transformation, comprising ORF12 (SEQ ID NO:201) and corresponding to the map in FIG. 13. Sequence in bold corresponds to the double CaMV35S promoter. Sequence in italics corresponds to ORF12 (SEQ ID NO: 201). Sequence underlined corresponds to the 3' terminator sequence from CaMV35S gene. Sequence in regular font corresponds to vector sequence.

[0179]FIG. 15 shows measurements of Electron Transfer Rate (ETR) and Quantum Yield of PSII (Quantum yield), measured with PAM2000, in the leaves of transgenic (C4-14, D4-5, D4-7, D4-32, D12-58, D12-60 and D12-61) and non-transgenic (TC2) perennial ryegrass lines

[0180]FIG. 16 shows phenotypic response at recovery stage following two cycles of drought (4-h drought, 6-days recovery, 8-hour drought, 20-h recovery) in transgenic (D4-5 bottom left, D12-58--above D4-5, and D4-7--above TC2) and non-transgenic (TC2--bottom right) perennial ryegrass lines

[0181]FIG. 17 shows phenotypic response of transgenic ryegrass lines (D4-1, D4-7, D12-60 and D12-61) in comparison to a non-transgenic line wildtype) at the end of 14-day drought-stress (left) and at the end of 1-day recovery from drought-stress (right)

[0182]FIG. 18 shows measurement of Volumetric Water Content (VWC) of the soil during the experimental stage in the SUNLIT chamber as measured with a TDR300.

[0183]FIG. 19 shows phenotypic response of transgenic and non-transgenic ryegrass lines in the SUNLIT chamber after 54 days of sub-surface irrigation

[0184]FIG. 20 shows measurements of Electron Transfer Rate (ETR) in the leaves of transgenic (C4-19, C4-20, D12-60, D12-61, D4-1 and D4-7) and non-transgenic (WT) perennial ryegrass lines grown in the SUNLIT chamber before drought-stress (white column) and at the end of the drought-stress (black column)

[0185]FIG. 21 shows measurements of Quantum Yield of PSII (Yield) in the leaves of transgenic (C4-19, C4-20, D12-60, D12-61, D4-1 and D4-7) and non-transgenic (WT) perennial ryegrass lines grown in the SUNLIT chamber before drought-stress (white column) and at the end of the drought-stress (black column)

[0186]FIG. 22 shows measurement of above ground biomass (dark bars represent fresh weight and light bars represent dry weight) and % mass loss, two days after re-watering, post drought regime for transgenic (C4-19, C4-20, D12-60, D12-61, D4-1 and D4-7) and non-transgenic (WT) perennial ryegrass lines.

[0187]FIG. 23 shows newly produced above ground biomass during the during the drought screen, for transgenic (C4-19, C4-20, D12-60, D12-61, D4-1 and D4-7) and non-transgenic (WT) perennial ryegrass lines.

DETAILED DESCRIPTION

[0188]The term "plant" is intended to include a whole plant, any part of a plant, propagules and progeny of a plant.

[0189]The term `propagule` means any part of a plant that may be used in reproduction or propagation, either sexual or asexual, including seeds and cuttings.

[0190]The term "tolerance or tolerant to drought stress" is intended to describe a plant or plants which perform more favourably in any aspect of their growth and development under sub-optimal hydration conditions than do suitable control plants in the same conditions.

[0191]The term "tolerance or tolerant to cold stress" is intended to describe a plant or plants which perform more favourably in any aspect of their growth and development under sub-optimal-reduced reduced temperature conditions than suitable control plants in the same conditions.

[0192]The term "tolerance or tolerant to freezing stress" is intended to describe a plant or plants which perform more favourably in any aspect of their growth and development under temperature conditions of less than or equal to 0° C., than do suitable control plants in the same conditions.

[0193]The term "tolerance or tolerant to heat stress" is intended to describe a plant or plants which perform more favourably in any aspect of their growth and development under sub-optimal elevated temperature conditions than do suitable control plants in the same conditions.

[0194]The term "tolerance or tolerant to salinity" is intended to describe a plant or plants which perform more favourably in any aspect of their growth and development under sub-optimal elevated salinity conditions than do in the same conditions.

[0195]Suitable control plants may include non-transformed plants of the same species and variety, or plants of the same species or variety transformed with a control construct.

[0196]With reference to the selection methods of the invention, a plant with increased tolerance to environmental stress refers to a plant, selected from a population of plants, which performs more favourably in any aspect of growth and development under stress conditions than does an average member of the population under the same conditions.

[0197]The term "comprising" as used in this specification and claims means "consisting at least in part of"; that is to say when interpreting statements in this specification and claims which include "comprising", the features prefaced by this term in each statement all need to be present but other features can also be present. Related terms such as "comprise" and "comprised" are to be interpreted in similar manner.

Polynucleotides and Fragments

[0198]The term "polynucleotide(s)," as used herein, means a single or double-stranded deoxyribonucleotide or ribonucleotide polymer of any length, and include as non-limiting examples, coding and non-coding sequences of a gene, sense and antisense sequences, exons, introns, genomic DNA, cDNA, pre-mRNA, mRNA, rRNA, siRNA, miRNA, tRNA, ribozymes, recombinant polynucleotides, isolated and purified naturally occurring DNA or RNA sequences, synthetic RNA and DNA sequences, nucleic acid probes, primers, and fragments.

[0199]A "fragment" of a polynucleotide sequence provided herein is a subsequence of contiguous nucleotides that is capable of specific hybridization to a target of interest, e.g., a sequence that is at least 15 nucleotides in length. The fragments of the invention comprise 15 nucleotides, preferably at least 20 nucleotides, more preferably at least 30 nucleotides, more preferably at least 50 nucleotides, more preferably at least 50 nucleotides and most preferably at least 60 nucleotides of contiguous nucleotides of a polynucleotide of the invention. A fragment of a polynucleotide sequence can be used in antisense, gene silencing, triple helix or ribozyme technology, or as a primer, a probe, included in a microarray, or used in polynucleotide-based selection methods of the invention.

[0200]The term "primer" refers to a short polynucleotide, usually having a free 3'OH group, that is hybridized to a template and used for priming polymerization of a polynucleotide complementary to the target.

[0201]The term "probe" refers to a short polynucleotide that is used to detect a polynucleotide sequence, that is complementary to the probe, in a hybridization-based assay. The probe may consist of a "fragment" of a polynucleotide as defined herein.

Polypeptides and Fragments

[0202]The term "polypeptide", as used herein, encompasses amino acid chains of any length, but preferably at least 5 amino acids in length, including full-length proteins, in which amino acid residues are linked by covalent peptide bonds. Polypeptides of the present invention may be purified natural products, or may be produced partially or wholly using recombinant or synthetic techniques. The term may refer to a polypeptide, an aggregate of a polypeptide such as a dimer or other multimer, a fusion polypeptide, a polypeptide fragment, a polypeptide variant, or derivative thereof

[0203]A "fragment" of a polypeptide is a subsequence of the polypeptide that performs a function that is required for the biological activity and/or provides three dimensional structure of the polypeptide. The term may refer to a polypeptide, an aggregate of a polypeptide such as a dimer or other multimer, a fusion polypeptide, a polypeptide fragment, a polypeptide variant, or derivative thereof capable of performing the above enzymatic activity.

[0204]The term "isolated" as applied to the polynucleotide or polypeptide sequences disclosed herein is used to refer to sequences that are removed from their natural cellular environment. An isolated molecule may be obtained by any method or combination of methods including biochemical, recombinant, and synthetic techniques.

[0205]The term "recombinant" refers to a polynucleotide sequence that is removed from sequences that surround it in its natural context and/or is recombined with sequences that are not present in its natural context.

[0206]A "recombinant" polypeptide sequence is produced by translation from a "recombinant" polynucleotide sequence.

[0207]The term "derived from" with respect to polynucleotides and polypeptides of the invention being "derived from" a particular genera or species, means that the polynucleotide or polypeptide has the same sequence as a polynucleotide or polypeptide found naturally in that genera or species. The polynucleotide or polypeptide which is derived from a genera or species may therefore be produced synthetically or recombinantly.

Variant

[0208]As used herein, the term "variant" refers to polynucleotide or polypeptide sequences different from the specifically identified sequences, wherein one or more nucleotides or amino acid residues is deleted, substituted, or added. Variants may be naturally occurring allelic variants, or non-naturally occurring variants. Variants may be from the same or from other species and may encompass homologues, paralogues and orthologues. In certain embodiments, variants of the inventive polypeptides and polynucleotides possess biological activities that are the same or similar to those of the inventive polypeptides or polynucleotides. The term "variant" with reference to polynucleotides and polypeptides encompasses all forms of polynucleotides and polypeptides as defined herein.

Polynucleotide Variants

[0209]Variant polynucleotide sequences preferably exhibit at least 50%, more preferably at least 51%, more preferably at least 52%, more preferably at least 53%, more preferably at least 54%, more preferably at least 55%, more preferably at least 56%, more preferably at least 57%, more preferably at least 58%, more preferably at least 59%, more preferably at least 60%, more preferably at least 61%, more preferably at least 62%, more preferably at least 63%, more preferably at least 64%, more preferably at least 65%, more preferably at least 66%, more preferably at least 67%, more preferably at least 68%, more preferably at least 69%, more preferably at least 70%, more preferably at least 71%, more preferably at least 72%, more preferably at least 73%, more preferably at least 74%, more preferably at least 75%, more preferably at least 76%, more preferably at least %, more preferably at least 77%, more preferably at least 78%, more preferably at least 79%, more preferably at least 80%, more preferably at least 81%, more preferably at least 82%, more preferably at least 83%, more preferably at least 84%, more preferably at least 85%, more preferably at least 86%, more preferably at least 87%, more preferably at least 88%, more preferably at least 89%, more preferably at least 90%, more preferably at least 91%, more preferably at least 92%, more preferably at least 93%, more preferably at least 94%, more preferably at least 95%, more preferably at least 96%, more preferably at least 97%, more preferably at least 98%, and most preferably at least 99% identity to a specified polynucleotide sequence. Identity is found over a comparison window of at least 20 nucleotide positions, preferably at least 50 nucleotide positions, more preferably at least 100 nucleotide positions, and most preferably over the entire length of the specified polynucleotide sequence.

[0210]Polynucleotide sequence identity can be determined in the following manner. The subject polynucleotide sequence is compared to a candidate polynucleotide sequence using BLASTN (from the BLAST suite of programs, version 2.2.5 [November 2002]) in bl2seq (Tatiana A. Tatusova, Thomas L. Madden (1999), "Blast 2 sequences--a new tool for comparing protein and nucleotide sequences", FEMS Microbiol Lett. 174:247-250), which is publicly available from NCBI (ftp://ftp.ncbi.nih.gov/blast/). The default parameters of bl2seq are utilized except that filtering of low complexity parts should be turned off

[0211]The identity of polynucleotide sequences may be examined using the following UNIX command line parameters: [0212]bl2seq nucleotideseq1 -j nucleotideseq2 -F F -p blastn

[0213]The parameter -F F turns off filtering of low complexity sections. The parameter -p selects the appropriate algorithm for the pair of sequences. The bl2seq program reports sequence identity as both the number and percentage of identical nucleotides in a line "Identities=".

[0214]Polynucleotide sequence identity may also be calculated over the entire length of the overlap between a candidate and subject polynucleotide sequences using global sequence alignment programs (e.g. Needleman, S. B. and Wunsch, C. D. (1970) J. Mol. Biol. 48, 443-453). A full implementation of the Needleman-Wunsch global alignment algorithm is found in the needle program in the EMBOSS package (Rice, P. Longden, I. and Bleasby, A. EMBOSS: The European Molecular Biology Open Software Suite, Trends in Genetics June 2000, vol 16, No 6. pp. 276-277) which can be obtained from http://www.hgmp.mrc.ac.uk/Software/EMBOSS/. The European Bioinformatics Institute server also provides the facility to perform EMBOSS-needle global alignments between two sequences on line at http:/www.ebi.ac.uk/emboss/align/.

[0215]Alternatively the GAP program may be used which computes an optimal global alignment of two sequences without penalizing terminal gaps. GAP is described in the following paper: Huang, X. (1994) On Global Sequence Alignment. Computer Applications in the Biosciences 10, 227-235.

[0216]Polynucleotide variants of the present invention also encompass those which exhibit a similarity to one or more of the specifically identified sequences that is likely to preserve the functional equivalence of those sequences and which could not reasonably be expected to have occurred by random chance. Such sequence similarity with respect to polynucleotides may be determined using the publicly available bl2seq program from the BLAST suite of programs (version 2.2.5 [November 2002]) from NCBI (ftp://ftp.ncbi.nih.gov/blast/).

[0217]The similarity of polynucleotide sequences may be examined using the following UNIX command line parameters: [0218]bl2seq -i nucleotideseq1 -j nucleotideseq2 -F F -p tblastx

[0219]The parameter -F F turns off filtering of low complexity sections. The parameter -p selects the appropriate algorithm for the pair of sequences. This program finds regions of similarity between the sequences and for each such region reports an "E value" which is the expected number of times one could expect to see such a match by chance in a database of a fixed reference size containing random sequences. The size of this database is set by default in the bl2seq program. For small E values, much less than one, the E value is approximately the probability of such a random match.

[0220]Variant polynucleotide sequences preferably exhibit an E value of less than 1×10^-10 more preferably less than 1×10^-20, more preferably less than 1×10^-30, more preferably less than 1×10^-40, more preferably less than 1×10^-50, more preferably less than 1×10^-60, more preferably less than 1×10^-70, more preferably less than 1×10^-80, more preferably less than 1×10^-90 and most preferably less than 1×10^-100 when compared with any one of the specifically identified sequences.

[0221]Alternatively, variant polynucleotides of the present invention hybridize to a specified polynucleotide sequence, or complements thereof under stringent conditions.

[0222]The term "hybridize under stringent conditions", and grammatical equivalents thereof, refers to the ability of a polynucleotide molecule to hybridize to a target polynucleotide molecule (such as a target polynucleotide molecule immobilized on a DNA or RNA blot, such as a Southern blot or Northern blot) under defined conditions of temperature and salt concentration. The ability to hybridize under stringent hybridization conditions can be determined by initially hybridizing under less stringent conditions then increasing the stringency to the desired stringency.

[0223]With respect to polynucleotide molecules greater than about 100 bases in length, typical stringent hybridization conditions are no more than 25 to 30° C. (for example, 10° C.) below the melting temperature (Tm) of the native duplex (see generally, Sambrook et al., Eds, 1987, Molecular Cloning, A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press; Ausubel et al., 1987, Current Protocols in Molecular Biology, Greene Publishing). Tm for polynucleotide molecules greater than about 100 bases can be calculated by the formula Tm=81.5+0.41% (G+C-log (Na+). (Sambrook et al., Eds, 1987, Molecular Cloning, A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press; Bolton and McCarthy, 1962, PNAS 84:1390). Typical stringent conditions for polynucleotide of greater than 100 bases in length would be hybridization conditions such as prewashing in a solution of 6×SSC, 0.2% SDS; hybridizing at 65° C., 6×SSC, 0.2% SDS overnight; followed by two washes of 30 minutes each in 1×SSC, 0.1% SDS at 65° C. and two washes of 30 minutes each in 0.2×SSC, 0.1% SDS at 65° C.

[0224]With respect to polynucleotide molecules having a length less than 100 bases, exemplary stringent hybridization conditions are 5 to 10° C. below Tm. On average, the Tm of a polynucleotide molecule of length less than 100 by is reduced by approximately (500/oligonucleotide length)° C.

[0225]With respect to the DNA mimics known as peptide nucleic acids (PNAs) (Nielsen et al., Science. 1991 Dec. 6; 254(5037):1497-500) Tm values are higher than those for DNA-DNA or DNA-RNA hybrids, and can be calculated using the formula described in Giesen et al., Nucleic Acids Res. 1998 Nov. 1; 26(21):5004-6. Exemplary stringent hybridization conditions for a DNA-PNA hybrid having a length less than 100 bases are 5 to 10° C. below the Tm.

[0226]Variant polynucleotides of the present invention also encompasses polynucleotides that differ from the sequences of the invention but that, as a consequence of the degeneracy of the genetic code, encode a polypeptide having similar activity to a polypeptide encoded by a polynucleotide of the present invention. A sequence alteration that does not change the amino acid sequence of the polypeptide is a "silent variation". Except for ATG (methionine) and TGG (tryptophan), other codons for the same amino acid may be changed by art recognized techniques, e.g., to optimize codon expression in a particular host organism.

[0227]Polynucleotide sequence alterations resulting in conservative substitutions of one or several amino acids in the encoded polypeptide sequence without significantly altering its biological activity are also included in the invention. A skilled artisan will be aware of methods for making phenotypically silent amino acid substitutions (see, e.g., Bowie et al., 1990, Science 247, 1306).

[0228]Variant polynucleotides due to silent variations and conservative substitutions in the encoded polypeptide sequence may be determined using the publicly available bl2seq program from the BLAST suite of programs (version 2.2.5 [November 2002]) from NCBI (ftp://ftp.ncbi.nih.gov/blast/) via the tblastx algorithm as previously described.

Polypeptide Variants

[0229]The term "variant" with reference to polypeptides encompass naturally occurring, recombinantly and synthetically produced polypeptides. Variant polypeptide sequences preferably exhibit at least 50%, more perferably at least 51%, more preferably at least 52%, more preferably at least 53%, more preferably at least 54%, more preferably at least 55%, more preferably at least 56%, more preferably at least 57%, more preferably at least 58%, more preferably at least 59%, more preferably at least 60%, more preferably at least 61%, more preferably at least 62%, more preferably at least 63%, more preferably at least 64%, more preferably at least 65%, more preferably at least 66%, more preferably at least 67%, more preferably at least 68%, more preferably at least 69%, more preferably at least 70%, more preferably at least 71%, more preferably at least 72%, more preferably at least 73%, more preferably at least 74%, more preferably at least 75%, more preferably at least 76%, more preferably at least 77%, more preferably at least 78%, more preferably at least 79%, more preferably at least 80%, more preferably at least 81%, more preferably at least 82%, more preferably at least 83%, more preferably at least 84%, more preferably at least 85%, more preferably at least 86%, more preferably at least 87%, more preferably at least 88%, more preferably at least 89%, more preferably at least 90%, more preferably at least 91%, more preferably at least 92%, more preferably at least 93%, more preferably at least 94%, more preferably at least 95%, more preferably at least 96%, more preferably at least 97%, more preferably at least 98%, and most preferably at least 99% identity to a sequences of the present invention. Identity is found over a comparison window of at least 20 amino acid positions, preferably at least 50 amino acid positions, more preferably at least 100 amin acid positions, and most preferably over the entir length of the specified polypeptide sequence.

[0230]Polypeptide sequence identity can be determined in the following manner. The subject polypeptide sequence is compared to a canidate polypeptide sequence using publicly available from NCBI (ftp://ftp.nvbi.nih.gov/blast/). The default parameters of bl2seq are utilized except that filtering of loq complexity regions should be turned off.

[0231]Polypeptide sequence identity may also be calculated over the entire length of the overlap between a canidate and subject polynucleotide sequences using global sequence alignment programs EMBOSS-needle (available at http:/www.ebi.ac.uk/emboss/align/) and GAP (Huang, X. (1994) On Global Sequence Alignment. Computer Applications in the Biosciences 10, 227-235.) as discussed above are also suitable global sequence alignment programs for calculating polypeptide sequence identity.

[0232]Polypeptide variants of the present invention also encompass those which exhibit a similarity to one or more of the specifically identified sequences that is likely to preserve the functional equivalence of those sequences and which could not reasonably be expected to have occurred by random chance. Such sequence similarity with respect to polypeptides may be determined using the publicly available bl2seq program from the BLAST suite of programs (version 2.2.5 [November 2002]) from NCBI (ftp://ftp.ncbi.nih.gov/blast/). The similarity of polypeptide sequences may be examined using the following UNIX command line parameters: [0233]bl2seq -i peptideseq1 -j peptideseq2 -F F -p blastp

[0234]Variant polypeptide sequences preferably exhibit an E value of less than 1×10^-10 more preferably less than 1×10^-20, more preferably less than 1×10^-30, more preferably less than 1×10^-40, more preferably less than 1×10^-50, more preferably less than 1×10^-60, more preferably less than 1×10^-70, more preferably less than 1×10^-80, more preferably less than 1×10^-90 and most preferably less than 1×10^-100 when compared with any one of the specifically identified sequences.

[0235]The parameter -F F turns off filtering of low complexity sections. The parameter -p selects the appropriate algorithm for the pair of sequences. This program finds regions of similarity between the sequences and for each such region reports an "E value" which is the expected number of times one could expect to see such a match by chance in a database of a fixed reference size containing random sequences. For small E values, much less than one, this is approximately the probability of such a random match.

[0236]Conservative substitutions of one or several amino acids of a described polypeptide sequence without significantly altering its biological activity are also included in the invention. A skilled artisan will be aware of methods for making phenotypically silent amino acid substitutions (see, e.g., Bowie et al., 1990, Science 247, 1306).

Constructs, Vectors and Components Thereof

[0237]The term "genetic construct" refers to a polynucleotide molecule, usually double-stranded DNA, which may have inserted into it another polynucleotide molecule (the insert polynucleotide molecule) such as, but not limited to, a cDNA molecule. A genetic construct may contain the necessary elements that permit transcribing the insert polynucleotide molecule, and, optionally, translating the transcript into a polypeptide. The insert polynucleotide molecule may be derived from the host cell, or may be derived from a different cell or organism and/or may be a recombinant polynucleotide. Once inside the host cell the genetic construct may become integrated in the host chromosomal DNA. The genetic construct may be linked to a vector.

[0238]The term "vector" refers to a polynucleotide molecule, usually double stranded DNA, which is used to transport the genetic construct into a host cell. The vector may be capable of replication in at least one additional host system, such as E. coli.

[0239]The term "expression construct" refers to a genetic construct that includes the necessary elements that permit transcribing the insert polynucleotide molecule, and, optionally, translating the transcript into a polypeptide. An expression construct typically comprises in a 5' to 3' direction: [0240]a) a promoter functional in the host cell into which the construct will be transformed, [0241]b) the polynucleotide to be expressed, and [0242]c) a terminator functional in the host cell into which the construct will be transformed.

[0243]The term "coding region" or "open reading frame" (ORF) refers to the sense strand of a genomic DNA sequence or a cDNA sequence that is capable of producing a transcription product and/or a polypeptide under the control of appropriate regulatory sequences. The coding sequence is identified by the presence of a 5' translation start codon and a 3' translation stop codon. When inserted into a genetic construct, a "coding sequence" is capable of being expressed when it is operably linked to promoter and terminator sequences.

[0244]"Operably-linked" means that the sequenced to be expressed is placed under the control of regulatory elements that include promoters, tissue-specific regulatory elements, temporal regulatory elements, enhancers, repressors and terminators.

[0245]The term "noncoding region" refers to untranslated sequences that are upstream of the translational start site and downstream of the translational stop site. These sequences are also referred to respectively as the 5' UTR and the 3' UTR. These regions include elements required for transcription initiation and termination and for regulation of translation efficiency.

[0246]Terminators are sequences, which terminate transcription, and are found in the 3' untranslated ends of genes downstream of the translated sequence. Terminators are important determinants of mRNA stability and in some cases have been found to have spatial regulatory functions.

[0247]The term "promoter" refers to nontranscribed cis-regulatory elements upstream of the coding region that regulate gene transcription. Promoters comprise cis-initiator elements which specify the transcription initiation site and conserved boxes such as the TATA box, and motifs that are bound by transcription factors.

[0248]A "transgene" is a polynucleotide that is taken from one organism and introduced into a different organism by transformation. The transgene may be derived from the same species or from a different species as the species of the organism into which the transgene is introduced.

[0249]An "inverted repeat" is a sequence that is repeated, where the second half of the repeat is in the complementary strand, e.g.,

TABLE-US-00013 (5')GATCTA . . . TAGATC(3') (3')CTAGAT . . . ATCTAG(5')

Read-through transcription will produce a transcript that undergoes complementary base-pairing to form a hairpin structure provided that there is a 3-5 by spacer between the repeated regions.

[0250]A "transgenic plant" refers to a plant which contains new genetic material as a result of genetic manipulation or transformation. The new genetic material may be derived from a plant of the same species as the resulting transgenic plant or from a different species.

[0251]The terms "to alter expression of" and "altered expression" of a polynucleotide or polypeptide of the invention, are intended to encompass the situation where genomic DNA corresponding to a polynucleotide of the invention is modified thus leading to altered expression of a polynucleotide or polypeptide of the invention. Modification of the genomic DNA may be through genetic transformation or other methods known in the art for inducing mutations. The "altered expression" can be related to an increase or decrease in the amount of messenger RNA and/or polypeptide produced and may also result in altered activity of a polypeptide due to alterations in the sequence of a polynucleotide and polypeptide produced.

[0252]The applicants have identified a polynucleotide from ryegrass (SEQ ID NO: 82) encoding a polypeptide (SEQ ID NO: 4) which modulates in plants, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity. The applicants have also identified polynucleotide variants of SEQ ID NO: 82 (SEQ ID NOs: 83-159) encoding polypeptide variants of SEQ ID NOs: 4 (SEQ ID NOs: 5-81) which modulate in plants, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity. The applicants identified consensus sequences (SEQ ID NO:1) present in all of polypeptides encoded by such polynucleotides, as shown in FIG. 1. Further the applicants have identified a consensus sequence (SEQ ID NO:2) specific to dicotyledonous polypeptide sequences (FIG. 2) and a consensus sequence (SEQ ID NO:3) specific to monocotyledonous sequences (FIG. 3).

[0253]The applicants have identified a polynucleotide from ryegrass (SEQ ID NO:201) encoding a polypeptide (SEQ ID NO: 163) which modulates in plants, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity. The applicants have also identified polynucleotide variants of SEQ ID NO: 201 (SEQ ID NOs: 202-238) encoding polypeptide variants of SEQ ID NOs: 163 (SEQ ID NOs: 164-200) which modulate in plants, tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity. The applicants identified consensus sequences (SEQ ID NO:160) present in all of polypeptides encoded by such polynucleotides, as shown in FIG. 4. Further the applicants have identified a consensus sequence (SEQ ID NO:161) specific to dicotyledonous polypeptide sequences (FIG. 5) and a consensus sequence (SEQ ID NO:162) specific to monocotyledonous sequences (FIG. 6).

[0254]The invention provides plants altered relative to wild-type plants in tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity. The invention provides both plants with both increased tolerance to the above and plants with decreased tolerance to above characteristic stresses. The invention also provides methods for the production or selection of such plants.

[0255]The polynucleotide molecules of the invention can be isolated by using a variety of techniques known to those of ordinary skill in the art. By way of example, such polynucleotides can be isolated through use of the polymerase chain reaction (PCR) described in Mullis et al., Eds. 1994 The Polymerase Chain Reaction, Birkhauser, incorporated herein by reference. The polynucleotides of the invention can be amplified using primers, as defined herein, derived from the polynucleotide sequences of the invention.

[0256]The polynucleotide fragments of the invention may be produced by techniques well-known in the art such as restriction endonuclease digestion and oligonucleotide synthesis.

[0257]A partial polynucleotide sequence may be used, in methods well-known in the art to identify the corresponding full length polynucleotide sequence. Such methods would include PCR-based methods, 5'RACE (Frohman Mass., 1993, Methods Enzymol. 218: 340-56) and hybridization-based method, computer/database-based methods. Further, by way of example, inverse PCR permits acquisition of unknown sequences, flanking the polynucleotide sequences disclosed herein, starting with primers based on a known region (Triglia et al., 1998, Nucleic Acids Res 16, 8186, incorporated herein by reference). The method uses several restriction enzymes to generate a suitable fragment in the known region of a gene. The fragment is then circularized by intramolecular ligation and used as a PCR template. Divergent primers are designed from the known region. In order to physically assemble full-length clones, standard molecular biology approaches can be utilized (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987).

[0258]It may be beneficial, when producing a transgenic plant from a particular species, to transform such a plant with a sequence or sequences derived from that species. The benefit may be to alleviate public concerns regarding cross-species transformation in generating transgenic organisms. Additionally when down-regulation of a gene is the desired result, it may be necessary to utilise a sequence identical (or at least highly similar) to that in the plant, for which reduced expression is desired. For these reasons among others, it is desirable to be able to identify and isolate orthologues of a particular gene in several different plant species. Variants (including orthologues) may be identified by the methods described.

Methods for Identifying Variants

Physical Methods

[0259]Variant polynucleotides may be identified using PCR-based methods (Mullis et al., Eds. 1994 The Polymerase Chain Reaction, Birkhauser). Typically, the polynucleotide sequence of a primer, useful to amplify variant polynucleotide molecules by PCR, may be based on a sequence encoding a conserved region of the corresponding amino acid sequence.

[0260]Alternatively library screening methods will be known to those skilled in the art (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987) may be employed. When identifying variants of the probe sequence hybridisation and/or wash stringency conditions will typically be reduced relative to when exact sequence matches are sought.

[0261]Polypeptide variants of the invention may be identified by physical methods, for example by screening expression libraries using antibodies raised against polypeptides of the invention (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987) or by identifying polypeptides from natural sources with the aid of such antibodies.

Computer Based Methods

[0262]The variant sequences of the invention, including both polynucleotide and polypeptide variants, may also be identified by computer-based methods well-known to those skilled in the art, using public domain sequence alignment algorithms and sequence similarity search tools to search sequence databases (public domain databases include Genbank, EMBL, Swiss-Prot, PIR and others). See, e.g., Nucleic Acids Res. 29: 1-10 and 11-16, 2001 for examples of online resources. Similarity searches retrieve and align target sequences for comparison with a sequence to be analyzed (i.e., a query sequence). Sequence comparison algorithms use scoring matrices to assign an overall score to each of the alignments.

[0263]An exemplary family of programs useful for identifying variants in sequence databases is the BLAST suite of programs (version 2.2.5 [November 2002]) including BLASTN, BLASTP, BLASTX, tBLASTN and tBLASTX, which are publicly available from (ftp://ftp.ncbi.nih.gov/blast/) or from the National Center for Biotechnology Information (NCBI), National Library of Medicine, Building 38A, Room 8N805, Bethesda, Md. 20894 USA. The NCBI server also provides the facility to use the programs to screen a number of publicly available sequence databases. BLASTN compares a nucleotide query sequence against a nucleotide sequence database. BLASTP compares an amino acid query sequence against a protein sequence database. BLASTX compares a nucleotide query sequence translated in all reading frames against a protein sequence database. tBLASTN compares a protein query sequence against a nucleotide sequence database dynamically translated in all reading frames. tBLASTX compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database. The BLAST programs may be used with default parameters or the parameters may be altered as required to refine the screen.

[0264]The use of the BLAST family of algorithms, including BLASTN, BLASTP, and BLASTX, is described in the publication of Altschul et al., Nucleic Acids Res. 25: 3389-3402, 1997.

[0265]The "hits" to one or more database sequences by a queried sequence produced by BLASTN, BLASTP, BLASTX, tBLASTN, tBLASTX, or a similar algorithm, align and identify similar portions of sequences. The hits are arranged in order of the degree of similarity and the length of sequence overlap. Hits to a database sequence generally represent an overlap over only a fraction of the sequence length of the queried sequence.

[0266]The BLASTN, BLASTP, BLASTX, tBLASTN and tBLASTX algorithms also produce "Expect" values for alignments. The Expect value (E) indicates the number of hits one can "expect" to see by chance when searching a database of the same size containing random contiguous sequences. The Expect value is used as a significance threshold for determining whether the hit to a database indicates true similarity. For example, an E value of 0.1 assigned to a polynucleotide hit is interpreted as meaning that in a database of the size of the database screened, one might expect to see 0.1 matches over the aligned portion of the sequence with a similar score simply by chance. For sequences having an E value of 0.01 or less over aligned and matched portions, the probability of finding a match by chance in that database is 1% or less using the BLASTN, BLASTP, BLASTX, tBLASTN or tBLASTX algorithm.

[0267]Multiple sequence alignments of a group of related sequences can be carried out with CLUSTALW (Thompson, J. D., Higgins, D. G. and Gibson, T. J. (1994) CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680, http://www-igbmc.u-strasbg.fr/BioInfo/ClustalW/Top.html) or T-COFFEE (Cedric Notredame, Desmond G. Higgins, Jaap Heringa, T-Coffee: A novel method for fast and accurate multiple sequence alignment, J. Mol. Biol. (2000) 302: 205-217)) or PILEUP, which uses progressive, pairwise alignments. (Feng and Doolittle, 1987, J. Mol. Evol. 25, 351).

[0268]Pattern recognition software applications are available for finding motifs or signature sequences. For example, MEME (Multiple Em for Motif Elicitation) finds motifs and signature sequences in a set of sequences, and MAST (Motif Alignment and Search Tool) uses these motifs to identify similar or the same motifs in query sequences. The MAST results are provided as a series of alignments with appropriate statistical data and a visual overview of the motifs found. MEME and MAST were developed at the University of California, San Diego.

[0269]PROSITE (Bairoch and Bucher, 1994, Nucleic Acids Res. 22, 3583; Hofmann et al., 1999, Nucleic Acids Res. 27, 215) is a method of identifying the functions of uncharacterized proteins translated from genomic or cDNA sequences. The PROSITE database (www.expasy.org/prosite) contains biologically significant patterns and profiles and is designed so that it can be used with appropriate computational tools to assign a new sequence to a known family of proteins or to determine which known domain(s) are present in the sequence (Falquet et al., 2002, Nucleic Acids Res. 30, 235). Prosearch is a tool that can search SWISS-PROT and EMBL databases with a given sequence pattern or signature.

[0270]The function of a variant polynucleotide of the invention in modulating tolerance to environmental stresses in a plant may be assessed by expressing the polynucleotide in a plant and for example, analyzing the effect on stress tolerance by methods provided in the Example section. Further plant transformation protocols for several species are known to those skilled in the art. A list of such protocols is provided herein.

Methods for Isolating Polypeptides

[0271]The polypeptides of the invention, including variant polypeptides, may be prepared using peptide synthesis methods well known in the art such as direct peptide synthesis using solid phase techniques (e.g. Stewart et al., 1969, in Solid-Phase Peptide Synthesis, WH Freeman Co, San Francisco Calif., or automated synthesis, for example using an Applied Biosystems 431A Peptide Synthesizer (Foster City, California). Mutated forms of the polypeptides may also be produced during such syntheses.

[0272]The polypeptides and variant polypeptides of the invention may also be purified from natural sources using a variety of techniques that are well known in the art (e.g. Deutscher, 1990, Ed, Methods in Enzymology, Vol. 182, Guide to Protein Purification).

[0273]Alternatively the polypeptides and variant polypeptides of the invention may be expressed recombinantly in suitable host cells and separated from the cells as discussed below.

Methods for Producing Constructs and Vectors

[0274]The genetic constructs of the present invention comprise one or more polynucleotide sequences of the invention and/or polynucleotides encoding polypeptides of the invention, and may be useful for transforming, for example, bacterial, fungal, insect, mammalian or plant organisms. The genetic constructs of the invention are intended to include expression constructs as herein defined.

[0275]Methods for producing and using genetic constructs and vectors are well known in the art and are described generally in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987; Ausubel et al., Current Protocols in Molecular Biology, Greene Publishing, 1987).

Methods for Producing Host Cells Comprising Constructs and Vectors

[0276]The invention provides a host cell which comprises a genetic construct or vector of the invention. Host cells may be derived from, for example, bacterial, fungal, insect, mammalian or plant organisms.

[0277]Host cells comprising genetic constructs, such as expression constructs, of the invention are useful in methods well known in the art (e.g. Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987; Ausubel et al., Current Protocols in Molecular Biology, Greene Publishing, 1987) for recombinant production of polypeptides of the invention. Such methods may involve the culture of host cells in an appropriate medium in conditions suitable for or conducive to expression of a polypeptide of the invention. The expressed recombinant polypeptide, which may optionally be secreted into the culture, may then be separated from the medium, host cells or culture medium by methods well known in the art (e.g. Deutscher, Ed, 1990, Methods in Enzymology, Vol 182, Guide to Protein Purification).

Methods for Producing Plant Cells and Plants Comprising Constructs and Vectors

[0278]The invention further provides plant cells which comprise a genetic construct of the invention, and plant cells modified to alter expression of a polynucleotide or polypeptide of the invention. Plants comprising such cells also form an aspect of the invention.

[0279]Tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity in a plant, may also be altered through methods of the invention. Such methods may involve the transformation of plant cells and plants, with a construct of the invention designed to alter expression of a polynucleotide or polypeptide which modulates for example, tolerance to drought stress, in such plant cells and plants. Such methods also include the transformation of plant cells and plants with a combination of the construct of the invention and one or more other constructs designed to alter expression of one or more polynucleotides or polypeptides which modulate for example, tolerance to drought stress in such plant cells and plants.

[0280]Methods for transforming plant cells, plants and portions thereof with polynucleotides are described in Draper et al., 1988, Plant Genetic Transformation and Gene Expression. A Laboratory Manual, Blackwell Sci. Pub. Oxford, p. 365; Potrykus and Spangenberg, 1995, Gene Transfer to Plants. Springer-Verlag, Berlin; and Gelvin et al., 1993, Plant Molecular Biol. Manual. Kluwer Acad. Pub. Dordrecht. A review of transgenic plants, including transformation techniques, is provided in Galun and Breiman, 1997, Transgenic Plants. Imperial College Press, London.

Methods for Genetic Manipulation of Plants

[0281]A number of plant transformation strategies are available (e.g. Birch, 1997, Ann Rev Plant Phys Plant Mol Biol, 48, 297). For example, strategies may be designed to increase expression of a polynucleotide/polypeptide in a plant cell, organ and/or at a particular developmental stage where/when it is normally expressed or to ectopically express a polynucleotide/polypeptide in a cell, tissue, organ and/or at a particular developmental stage which/when it is not normally expressed. The expressed polynucleotide/polypeptide may be derived from the plant species to be transformed or may be derived from a different plant species.

[0282]Transformation strategies may be designed to reduce expression of a polynucleotide/polypeptide in a plant cell, tissue, organ or at a particular developmental stage which/when it is normally expressed. Such strategies are known as gene silencing strategies.

[0283]Genetic constructs for expression of genes in transgenic plants typically include promoters for driving the expression of one or more cloned polynucleotide, terminators and selectable marker sequences to detest presence of the genetic construct in the transformed plant.

[0284]The promoters suitable for use in the constructs of this invention are functional in a cell, tissue or organ of a monocot or dicot plant and include cell-, tissue- and organ-specific promoters, cell cycle specific promoters, temporal promoters, inducible promoters, constitutive promoters that are active in most plant tissues, and recombinant promoters. Choice of promoter will depend upon the temporal and spatial expression of the cloned polynucleotide, so desired. The promoters may be those normally associated with a transgene of interest, or promoters which are derived from genes of other plants, viruses, and plant pathogenic bacteria and fungi. Those skilled in the art will, without undue experimentation, be able to select promoters that are suitable for use in modifying and modulating plant traits using genetic constructs comprising the polynucleotide sequences of the invention. Examples of constitutive plant promoters include the CaMV 35S promoter, the nopaline synthase promoter and the octopine synthase promoter, and the Ubi 1 promoter from maize. Plant promoters which are active in specific tissues, respond to internal developmental signals or external abiotic or biotic stresses are described in the scientific literature. Exemplary promoters are described, e.g., in WO 02/00894, which is herein incorporated by reference.

[0285]Exemplary terminators that are commonly used in plant transformation genetic construct include, e.g., the cauliflower mosaic virus (CaMV) 35S terminator, the Agrobacterium tumefaciens nopaline synthase or octopine synthase terminators, the Zea mays zein gene terminator, the Oryza sativa ADP-glucose pyrophosphorylase terminator and the Solanum tuberosum PI-II terminator.

[0286]Selectable markers commonly used in plant transformation include the neomycin phophotransferase II gene (NPT II) which confers kanamycin resistance, the aadA gene, which confers spectinomycin and streptomycin resistance, the phosphinothricin acetyl transferase (bar gene) for Ignite (AgrEvo) and Basta (Hoechst) resistance, and the hygromycin phosphotransferase gene (hpt) for hygromycin resistance.

[0287]Use of genetic constructs comprising reporter genes (coding sequences which express an activity that is foreign to the host, usually an enzymatic activity and/or a visible signal (e.g., luciferase, GUS, GFP) which may be used for promoter expression analysis in plants and plant tissues are also contemplated. The reporter gene literature is reviewed in Herrera-Estrella et al., 1993, Nature 303, 209, and Schrott, 1995, In: Gene Transfer to Plants (Potrykus, T., Spangenberg. Eds) Springer Verlag. Berline, pp. 325-336.

[0288]Gene silencing strategies may be focused on the gene itself or regulatory elements which effect expression of the encoded polypeptide. "Regulatory elements" is used here in the widest possible sense and includes other genes which interact with the gene of interest.

[0289]Genetic constructs designed to decrease or silence the expression of a polynucleotide/polypeptide of the invention may include an antisense copy of a polynucleotide of the invention. In such constructs the polynucleotide is placed in an antisense orientation with respect to the promoter and terminator.

[0290]An "antisense" polynucleotide is obtained by inverting a polynucleotide or a segment of the polynucleotide so that the transcript produced will be complementary to the mRNA transcript of the gene, e.g.,

TABLE-US-00014 5'GATCTA 3' 3'CTAGAT 5' (coding strand) (antisense strand) 3'CUAGAU 5' 5'GAUCUA 3' mRNA antisense RNA

[0291]Genetic constructs designed for gene silencing may also include an inverted repeat as herein defined. The preferred approach to achieve this is via RNA-interference strategies using genetic constructs encoding self-complementary "hairpin" RNA (Wesley et al., 2001, Plant Journal, 27: 581-590).

[0292]The transcript formed may undergo complementary base pairing to form a hairpin structure. Usually a spacer of at least 3-5 by between the repeated regions is required to allow hairpin formation.

[0293]Another silencing approach involves the use of a small antisense RNA targeted to the transcript equivalent to an miRNA (Llave et al., 2002, Science 297, 2053). Use of such small antisense RNA corresponding to polynucleotide of the invention is expressly contemplated.

[0294]The term genetic construct as used herein also includes small antisense RNAs and other such polynucleotides effecting gene silencing.

[0295]Transformation with an expression construct, as herein defined, may also result in gene silencing through a process known as sense suppression (e.g. Napoli et al., 1990, Plant Cell 2, 279; de Carvalho Niebel et al., 1995, Plant Cell, 7, 347). In some cases sense suppression may involve over-expression of the whole or a partial coding sequence but may also involve expression of non-coding region of the gene, such as an intron or a 5' or 3' untranslated region (UTR). Chimeric partial sense constructs can be used to coordinately silence multiple genes (Abbott et al., 2002, Plant Physiol. 128(3): 844-53; Jones et al., 1998, Planta 204: 499-505). The use of such sense suppression strategies to silence the expression of a polynucleotide of the invention is also contemplated.

[0296]The polynucleotide inserts in genetic constructs designed for gene silencing may correspond to coding sequence and/or non-coding sequence, such as promoter and/or intron and/or 5' or 3' UTR sequence, or the corresponding gene.

[0297]Other gene silencing strategies include dominant negative approaches and the use of ribozyme constructs (McIntyre, 1996, Transgenic Res, 5, 257)

[0298]Pre-transcriptional silencing may be brought about through mutation of the gene itself or its regulatory elements. Such mutations may include point mutations, frameshifts, insertions, deletions and substitutions.

[0299]The following are representative publications disclosing genetic transformation protocols that can be used to genetically transform the following plant species: Rice (Alam et al., 1999, Plant Cell Rep. 18, 572); maize (U.S. Pat. Nos. 5,177,010 and 5,981,840); wheat (Ortiz et al., 1996, Plant Cell Rep. 15, 1996, 877); tomato (U.S. Pat. No. 5,159,135); potato (Kumar et al., 1996 Plant J. 9, : 821); cassava (Li et al., 1996 Nat. Biotechnology 14, 736); lettuce (Michelmore et al., 1987, Plant Cell Rep. 6, 439); tobacco (Horsch et al., 1985, Science 227, 1229); cotton (U.S. Pat. Nos. 5,846,797 and 5,004,863); grasses (U.S. Pat. Nos. 5,187,073 and 6,020,539); peppermint (Niu et al., 1998, Plant Cell Rep. 17, 165); citrus plants (Pena et al., 1995, Plant Sci. 104, 183); caraway (Krens et al., 1997, Plant Cell Rep, 17, 39); banana (U.S. Pat. No. 5,792,935); soybean (U.S. Pat. Nos. 5,416,011; 5,569,834; 5,824,877; 5,563,04455 and 5,968,830); pineapple (U.S. Pat. No. 5,952,543); poplar (U.S. Pat. No. 4,795,855); monocots in general (U.S. Pat. Nos. 5,591,616 and 6,037,522); brassica (U.S. Pat. Nos. 5,188,958; 5,463,174 and 5,750,871); and cereals (U.S. Pat. No. 6,074,877). Other species are contemplated and suitable methods and protocols are available in the scientific literature for use by those skilled in the art.

[0300]Several further methods known in the art may be employed to alter expression of a nucleotide and/or polypeptide of the invention. Such methods include but are not limited to Tilling (Till et al., 2003, Methods Mol Biol, 2%, 205), so called "Deletagene" technology (Li et al., 2001, Plant Journal 27(3), 235) and the use of artificial transcription factors such as synthetic zinc finger transcription factors. (e.g. Jouvenot et al., 2003, Gene Therapy 10, 513). Additionally antibodies or fragments thereof, targeted to a particular polypeptide may also be expressed in plants to modulate the activity of that polypeptide (Jobling et al., 2003, Nat. Biotechnol., 21(1), 35). Transposon tagging approaches may also be applied. Additionally peptides interacting with a polypeptide of the invention may be identified through technologies such as phase-display (Dyax Corporation). Such interacting peptides may be expressed in or applied to a plant to affect activity of a polypeptide of the invention. Use of each of the above approaches in alteration of expression of a nucleotide and/or polypeptide of the invention is specifically contemplated.

Methods for Plants

[0301]Methods are also provided for selecting plants altered tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity. Such methods involve testing of plants for altered for the expression of a polynucleotide or polypeptide of the invention. Such methods may be applied at a young age or early developmental stage to accelerate breeding programs directed toward at least one of the characteristics described which may not be easily assessed until a later age or developmental stage.

[0302]The expression of a polynucleotide, such as a messenger RNA, is often used as an indicator of expression of a corresponding polypeptide. Exemplary methods for measuring the expression of a polynucleotide include but are not limited to Northern analysis, RT-PCR and dot-blot analysis (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987). Polynucleotides or portions of the polynucleotides of the invention are thus useful as probes or primers, as herein defined, in methods for the identification of plants with altered tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity. For example an altered level in a plant, of a polypeptide involved in modulating tolerance to drought stress may be used as an indicator of eventual tolerance to drought stress in such a plant. The polynucleotides of the invention may be used as probes in hybridization experiments, or as primers in PCR based experiments, designed to identify such plants.

[0303]Alternatively antibodies may be raised against polypeptides of the invention. Methods for raising and using antibodies are standard in the art (see for example: Antibodies, A Laboratory Manual, Harlow A Lane, Eds, Cold Spring Harbour Laboratory, 1998). Such antibodies may be used in methods to detect altered expression of polypeptides which modulate flower size in plants. Such methods may include ELISA (Kemeny, 1991, A Practical Guide to ELISA, NY Pergamon Press) and Western analysis (Towbin & Gordon, 1994, J Immunol Methods, 72, 313).

[0304]These approaches for analysis of polynucleotide or polypeptide expression and the selection of plants with altered expression are useful in conventional breeding programs designed to produce varieties with altered in tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity.

Plants

[0305]The plants of the invention may be grown and either selfed or crossed with a different plant strain and the resulting hybrids, with the desired phenotypic characteristics, may be identified. Two or more generations may be grown to ensure that the subject phenotypic characteristics are stably maintained and inherited. Plants resulting from such standard breeding approaches also form an aspect of the present invention.

Examples

[0306]The invention will now be illustrated with reference to the following non-limiting examples.

Example 1

Identification of Polynucleotides Which Modulate Tolerance to Environmental Stresses

Introduction

[0307]Transcript identification in different tissue and developmental stages is based mainly by the use of EST (expressed sequence tag)-based methods (Adams et al. 1991, Science 252:1651-1656). SAGE (serial analysis of gene expression) (Velculescu et al. 1995, Science 270: 484-487) is a modification of conventional EST methods and has many advantages over that of other methods, such as microarray (Richmond and Somerville 2000, Current Opinion in Plant Biology. 3:108-116). Transcript profiling by SAGE is not limited to studying known genes and is estimated to be 26 times more sensitive than EST method (Sun et al. 2004, BMC Genomics 5: 1.1-1.4). Unlike microarray, SAGE is free from cross-hybridization problem and accurate because of dimer-formation prior to amplification (Ruan et al. 2004, Trends in Biotechnology 22: 23-30.). Alternative splicing of the transcript is a well-known phenomenon, whose impact is not fully understood yet (Lee et al. 2003, PNAS 99:12257-12262). These splice variants can be identified by SAGE. SAGE is also able to detect antisense RNAs, since the orientation of the SAGE tag on the transcripts can be readily determined. Antisense transcripts are likely to represent novel genes whose function may or may not be related to regulation of the expression of the genes transcribed from the sense strand (Chen et al. 2002, Nucleic Acids Res. 31:101-105). Although SAGE is widely used in the analysis of transcriptomes from cancerous human cells, it is not widely applied in other organisms, including plants. To date only a handful of papers have appeared where SAGE has been applied to characterize plant transcriptome, for example; Lee and Lee, 2003 Plant Physiol. 132: 517-529. Most of the plant transcriptome analysed using SAGE has been confined to the two fully sequenced genomes, namely Arabidopsis and rice.

[0308]Perennial ryegrass (Lolium perenne L.) is a cool temperate pasture plant from the family Gramineae and the tribe Festucaceae. To generate a profile of relative gene expression patterns in ryegrass, RNA was extracted from samples obtained from ambient temperature growth, cold grown, hydrated, dehydrated and rehydrated or dehydration pre- and post-grazed plants during autumn, summer, spring and winter, and used for constructing a SAGE library.

Materials and Methods:

[0309]Perennial ryegrass (Lohum perenne L.) cv. Bronsyn was used throughout this study. Field grown samples were collected from active paddocks at Dexcel, Hamilton, New Zealand during the peak of each season. Grass samples were collected from pre-grazed (15 days post grazing) and post-grazed (1 day post grazing) ryegrass swards. Tufts of grass samples were harvested from 3-6 randomly chosen sites and stored in dry-ice after snap-freezing with liquid nitrogen. During spring, immature spike and floral initials were also harvested. For stress-treatment, the following conditions were used on lab-grown ryegrass: Mature lab-grown perennial ryegrass that was grown in growth chamber for 15 months at 85% RH, 20° C./18° C. and 16 h/8 h day/night regime; Hydrated control grown for 55 days at 85% RH, 20° C./18° C. and 16 h/8 h day/night regime; 6 days at 70% RH, 22° C./16° C. and 16 h/8 h day/night regime, seedlings were kept watered throughout their life; Dehydrated sample watered only for 55 days at 85% RH, 20° C./18° C. and 16 h/8 h day/night regime; 3 days at 70% RH, 28° C./20° C. and 16 h/8 h day/night regime; 3 days at 50% RH, 28° C./20° C. and 16 h/8 h day/night regime; Rehydrated samples were from dehydrated plants that was watered for 24 hours and grown at 70% RH, 22° C./16° C. and 16 h/8 h day/night regime; Cold-stressed plants were grown for 55 days at 85% RH, 20° C./18° C. and 16 h/8 h day/night regime; 7 days at 70% RH, 22° C./16° C. and 16 h/8h day/night regime; 7 days at 70% RH, 6° C./2° C. and 16 h/8 h day/night regime, seedlings were kept watered throughout their life.

Construction of SAGE Libraries

[0310]RNA was extracted using TRIZOL® reagent (Invitrogen, CA, USA) and by the protocol described by the manufacturer from tissue that was ground in liquid nitrogen. For each SAGE library 100 μg of total RNA was used and the libraries were created using I-SAGE® or I-SAGE® Long kit (Invitrogen, CA, USA) according to manufacturer's protocol. From each library 960-1,920 clones were sequenced (Australian Genome Research Facility, Brisbane, Australia) and the tags extracted using the SAGE2000 software.

SAGE Bioinformatics:

[0311]The relational database was designed to hold tags, libraries and expression counts of the SAGE experiments. Each tag sequence (including enzyme sequence) was searched against the whole Ryegrass non-overlapping Gene thresher and the EST sets. The search was carried out in both direction and used exact match only. Results were loaded to the relational database using General Feature Format (GFF) approach (http://www3.ebi.ac.uk/Services/WebFeat)

[0312]All Ryegrass Gene thresher and the EST sequences were annotated using homology searches against some or all the following public and propriety databases: [0313]AGI TIGR Gene Indices, Arabidopsis, release 11, Jan. 2004 [0314]OGI TIGR Gene Indices, Rice, release 14-1, Jan. 2004 [0315]GENESEQN Derwent patent DNA sequences Dec. 7, 2002 [0316]GENESEQP Derwent patent amino acid sequences Dec. 7, 2002 [0317]Os_unigene Oryza sativa Unigene unique sequences Mar. 18, 2004 [0318]est_others Other EST sequences (mammal, fungi, prokaryote) Mar. 11, 2003 [0319]est_plant Viridiplantae subset of Non-redundant Database of GenBank+EMBL+DDBJ EST Divisions Mar. 3, 2004 [0320]nr All non-redundant GenBank CDS translations+PDB+SwissProt+PIR Mar. 11, 2003 [0321]nr_plant Plant subset of HS subset of BT subset of All non-redundant GenBank CDS translations+PDB+SwissProt+PIR Aug. 8, 2003 [0322]nt All Non-redundant GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS, or HTGS sequences) Mar. 11, 2003 [0323]nt₁₃ monocots Monocot subset of All Non-redundant GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS, or HTGS sequences) Mar. 11, 2003 [0324]swissprot The last major release of the SWISS-PROT protein sequence database (no updates) Mar. 28, 2003

[0325]A cutoff of E value less than E-05 was used and maximum of 10 targets per database were stored in the relational database.

Tags Annotation:

[0326]Tags with hits to the Ryegrass sets were annotated by creating a summary of all the annotations of the involved sequences. The summary was generated using an algorithm to calculate the frequency of the occurrence of each word in the annotations and rank them in descending order based on the number off occurrences. The summary was limited to 10 words and a void word list was used to filter out insignificant information. The resulting summary line was used as an indication of what the tags were likely to be. Actual numbers are displayed; giving additional information that could be used to evaluate the significance of each of the words in the summary. This method of automatic annotation using keyword counts is similar to the Automatic comment that is used by the ProDom database (http://protein.toulouse.inra.fr/prodom/current/html/ home.php) to annotate the automatically generated protein domain families.

[0327]Detailed annotation based on the top hits of the involved sequences was displayed when viewing tags data.

[0328]Two polynucleotide sequences of particular interest were identified in the above analysis. These are ORF4 (corresponds to SEQ ID NO: 82) and ORF12 (corresponding to SEQ ID NO: 201).

[0329]ORF4 appears to encode a helix-loop-helix transcription factor. The transcript accumulates in cold, dehydrated and rehydrating tissues. The full transcript profile is shown in table 1.

TABLE-US-00015 TABLE 1 SAGE_TAG TGACACCGTT LPWPRE 0 LPWPOS 0 LPRWIN 0 LPSPRE 0 LPSPOS 2 LPISFI 1 LPUPOS 2 LPAPRE 2 LPAPOS 1 LPMALF 0 LPCOLD 2 LPHYDR 0 LPDEHY 4 LPREHY 2 Total 15

[0330]ORF12 appears to encode a zinc finger protein and the transcript accumulates in response to drought stimulus. The full transcript profile is shown in table 2 below.

TABLE-US-00016 TABLE 2 SAGE_TAG TGTATCATTA LPWPRE 0 LPWPOS 0 LPRWIN 0 LPSPRE 0 LPSPOS 0 LPISFI 0 LPUPOS 0 LPAPRE 0 LPAPOS 0 LPMALF 2 LPCOLD 0 LPHYDR 0 LPDEHY 6 LPREHY 0 Total 8

[0331]ORF12 appears to be a C2H2 class zinc finger transcription factor with two zinc fingers. The first zinc finger is contained in the polypeptide between amino acid residue 81 and 101 of SEQ ID NO:163 while the second zinc finger is contained in the polypeptide sequence between amino acid residue 144 and 164. Within the first zinc finger a conserved amino acid sequence motif QALGGHK was identified which is directly repeated in the second zinc finger domain, the conserved motif being separated by 56 residues. The H residue in this motif, in the first zinc finger, appears to be the first of the two H residues that is reported to be the active site in the C2H2 class zinc finger transcription factors.

Example 2

Identification Variants of ORF 4 and ORF 12

[0332]The polypeptide sequence encoded by the ORF4 and ORF12 were used as seed sequences to perform BLASTP search against NR PLANT database (release date Jul. 30, 04). Besides BLASTP, a TBLASTN search was also performed against EST PLANT database (release date Jul. 15, 04) and NT PLANT database (release date Jul. 15, 04). To identify the variants cut-off e value was generally set at greater than 1e-05, which was determined based upon the associated score value.

[0333]Selected variant sequences were aligned using the EMBOSS tool EMMA (Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994, CABIOS, 10, 19-29.), which is an interface to the popular multiple alignment program ClustalW. Aligned sequences were visualised using another EMBOSS tool called prettyplot, which displays aligned sequences with colouring and boxing.

[0334]All the ORF4 variant polypeptide sequences above were aligned as described above with ORF4 and a consensus motif (SEQ ID NO:1) common to ORF4 and ORF4 variants from all plant (both monocotyledonous and dicotyledonous) sequences was identified as shown in FIG. 1.

[0335]A similar consensus motif (SEQ ID NO:2) was identified which was specific to ORF4 and all of the dicotyledonous variant sequences as shown in FIG. 2.

[0336]A further consensus motif (SEQ ID NO:3) was identified which was specific to ORF4 and all of the monocotyledonous variant sequences as shown in FIG. 3.

[0337]All the ORF12 variant polypeptide sequences above were aligned as described above with ORF12 and a consensus motif (SEQ ID NO:4) common to ORF12 and ORF12 variants from all plant (both monocotyledonous and dicotyledonous) sequences was identified as shown in FIG. 4.

[0338]A similar consensus motif (SEQ ID NO:5) was identified which was specific to ORF4 and all of the dicotyledonous variant sequences as shown in FIG. 5.

[0339]A further consensus motif (SEQ ID NO:6) was identified which was specific to ORF4 and all of the monocotyledonous variant sequences as shown in FIG. 6.

[0340]We note that the repeated QALGGHK motif discussed above which was identified in ORF12 is also repeated in all of the ORF12 polypeptide variants being separated by between 36 and 63 amino acid residues. This is thus a distinctive feature of ORF12 and all of the ORF12 variant sequences identified.

Example 3

Preparation of Vectors Comprising Polynucleotides of the Invention for Plant Transformation

Vectors Comprising ORF4

[0341]A vector comprising ORF4 driven by the ryegrass promoter of SEQ ID NO:239 was produced by standard molecular biology techniques. A map to vectors is shown in FIG. 7. The sequence of the vector (SEQ ID NO:240) is shown in FIG. 8.

[0342]A vector comprising ORF4 driven by the double CaMV35S promoter was produced by standard molecular biology techniques. A map to vectors is shown in FIG. 11. The sequence of the vector (SEQ ID NO:242) is shown in FIG. 12.

Vector Comprising ORF12

[0343]A vector comprising ORF12 driven the by the ryegrass promoter of SEQ ID NO:239 was produced by standard molecular biology techniques. A map of the vector is shown in FIG. 9. The sequence (SEQ ID NO:241) and features of the vector is shown in FIG. 10.

[0344]A vector comprising ORF12 driven the by the double CaMV35S promoter was produced by standard molecular biology techniques. A map of the vector is shown in FIG. 13. The sequence (SEQ ID NO:243) and features of the vector is shown in FIG. 14.

Example 4

Transformation of Plants Within the Polynucleotides of the Invention

Donor Plant Production to Obtain Tissue Culture Explants

[0345]Seeds to establish contamination free in-vitro cultures were surface sterilized for 3 minutes with 70% (v/v) ethanol; followed by 60 minutes with sodium hypochlorite solution (2.4% active Chlorine) supplemented with a surfactant (0.1% (w/v) of Tween-20 and five rinses with autoclaved distilled water. Plantlets of perennial ryegrass (Lolium perenne L.) cultivar `Limes` (DSV Lippstadt/Germany) were clonally propagated in a 90 mm Petridish, containing Murashige and Skoog (Murashige, T and Skoog, F (1962) Physiol. Plant 15(3): 473-497) basal medium supplemented with 0.1 mg/l Benzylaminopurine; pH 5.8 and was solidified with 3.0g/l phytagel (Sigma), at 16° C. during night and 20° C. during the day with 14/12 h light/dark cycle. Light intensity of at least 360 μEm^-2s^-1 at plant height was maintained with sodium vapor lights (SON-T AGRO 400, Phillips). Axillary buds approximately 4-10 mm in size were excised and placed on callus induction medium. 12 explants were cultured per 90 mm petri-dish at 20 μEm^-2s^-1 and 25° C. for 28 to 56 days and calli sub-cultured to fresh medium every 14 days.

Biolistic Gene Transfer, Selection and Regeneration of Transgenic Plants

[0346]Calli were bombarded with DNA-coated particles six to ten weeks after culture of explants. Four to six hours prior to biolistic gene transfer calli were sub-cultured on medium with additional 64 gl^-1 mannitol and retransferred to mannitol free callus subculture medium after the particle bombardment. Regeneration medium differed from the callus induction medium in the phytohormone composition (no 2,4-D and BAP) and the carbohydrate source and concentration (20 g-1^-1 sucrose). Calli were cultured in low light at 20 μm^-2s^-1 and 24° C. and regenerated initially at 50 μm^-2s^-1 with a 16 h day, 8 h night cycle at 24° C. Two weeks after transfer to regeneration media light intensity was increased to 130 μm^-2s^-1 with fluorescent lamps (Philips TL-D 58 W/840R).

[0347]The plasmid pJFnpt contains the selectable nptII gene, encoding the enzyme neomycin phosphotransferase II under control of the maize ubiquitin promoter and first intron (Christensen and Quail 1996, Transgenic Res., 5, 213-218). The nptII expression cassette from pJFnpt was inserted into the pPZP 111 vector [P Hajdukiewicz, Z Svab, and P Maliga, 1994 Plant Molecular Biology 25: 989-994]. The plasmids pPZP 111, pCORF4, pCORF12, pDORF4 and pDORF12 were isolated as supercoiled DNA using commercially available DNA Maxiprep Kit. Vector backbone was removed from both the selectable marker gene expression cassette as well as from the target gene expression cassette by restriction digest, gel electrophoresis and gel purification prior gene transfer. Genetic transformation of perennial ryegrass was essentially carried out as described previously (Altpeter, F., Xu, J. and Ahmed S. 2000, Molecular Breeding, 6, 519-528). In brief, minimal transgene expression cassettes without vector backbone were precipitated on gold particles and delivered to target tissue in a 2:1 molar ratio (target gene expression cassette: selectable marker gene expression cassette) using a DuPont PDS-1000/He (BioRad, USA) device and 1100 psi rupture disks [Kikkert, J. R., 1993, Plant Cell, Tissue and Organ Culture, 33.(3) 221-226]. Particle density was adjusted by the final volume of ethanol in the gold-DNA suspension to 50 μg per bombardment. Five μl of the DNA coated particles were spread on the surface of the macrocarrier. Thirty to 35 callus pieces were put in the center of a petridish per bombardment six to ten weeks after callus initiation.

[0348]Selection was initiated five to seven days after biolistic gene transfer into calli. Two to three biweekly callus subcultures on CIM medium with 50 mgl^-1 paromomycin were followed by two to three biweekly subcultures on 50 mg l-1 paromomycin containing SRM medium. Four to eight weeks after transfer of selected calli to light, rooted transgenic plants were screened by performing an ELISA for nptII expression using leaf protein extracts. nptII positive plants were further screened by performing a genomic PCR involving ORF4 or ORF12 specific primers as appropriate. Positive primary transformants were transferred to soil under controlled environment conditions and kept at 15° C./12° C. day/night with a 12 hour photoperiod and 400 μEm^-2s^-1. Illumination was provided by sodium vapor lamps (Philips SON-T AGRO 400) and vegetatively propagated to produce clones of uniform size and growth. RT-PCR was carried out using standard methodology on regenerated plants to determine the transgene expression levels and lines for drought screening were selected based on the transgene expression level.

[0349]Transgenic lines transformed with the double CaMV 35-driven ORF4 cassette used in further experiments included: C4 14, C4-19 and C4-20.

[0350]Transgenic lines transformed with the ryegrass promoter (SEQ ID NO:239)-driven ORF4 cassette, used in further experiments included D4-1, D4-5, D4-7 and D4-32.

[0351]Transgenic lines transformed with the ryegrass promoter (SEQ ID NO:239)-driven ORF12 cassette, used in further experiments included D12-58, D12-60 and D12-61.

Example 5

Alteration in Tolerance to Environmental Stress in Plants Transformed with Polynucleotides of the Invention

Drought Screening in Growth Chamber Based Hydroponics System.

[0352]Clones of selected lines and a non-transgenic control line were established in a hydroponics system that was set up in a growth chamber. The experimental setup involved four replications. After establishment, the plants were exposed to two rounds of drought-stress (plants lifted up from the hydroponic system) comprising of 4 h drought followed by 6 days of recovery in the first cycle and then by 8 hours of drought and 20 h recovery in the second cycle. Biometric parameters such as Quantum yield of Photosystem II (yield) and Electron Transfer Rate (ETR) were measured using a Pulse Modulated Fluorometer (PAM2000) before the drought stress, at the end of the first drought cycle, at the end of the first recovery period, at the end of the second drought stress and finally at the end of the second recovery period (FIG. 15). Each data point in the figure represents the average of 12 measurements (three measurements per plant and four plants per line). The non-transgenic control fared poorly as when compared with its transgenic counterparts expressing either ORF4 or ORF12. FIG. 16 shows the condition of non-transgenic plant (TC 2, bottom right) and some of the transgenic lines at the end of the second recovery period.

Drought Screening in Glasshouse Based Potted Plants.

[0353]Equal sized transgenic and non-transgenic clones were produced and established in pots filled with soil. Drought screening was carried out by withholding water for fourteen days and then the recovery observed after a day after irrigating the plants. Once again non-transgenic plants fared worse than the transgenic lines expressing either ORF4 or ORF12. FIG. 17 is a representative image of the outcome of this trial.

Drought Screening in SUN-Lit Chambers

[0354]Transgenic lines over-expressing ORF 4, or 12 were selected for a detailed physiological analysis in SUN-LIT chambers following their performance in hydroponic culture and soil (pots) under controlled environment conditions (growth chamber and greenhouse respectively). Six lines of transgenic ryegrass and a wildtype ryegrass (WT) were vegetatively propagated in the greenhouse before transplanting to the SPAR chamber, i.e., C4-19, C4-20, D4-1, D4-7, D12-60, D12-61, non-transgenic WT. These lines were randomized in a block design of 4 replications per chamber.

Soil Moisture Monitoring

[0355]The soil moisture (VWC, volumetric water content) was recorded with a TDR300 at an interval of two to three days starting 10 Feb. 2006. Measurements were taken in each row between each of the plants at 20 cm depth (there were 28 positions for monitoring soil water status in each chamber). The time course of the VWC (every data point represents the VWC average of 28 positions) is shown in FIG. 18 and the dates for subsurface irrigation are indicated. Following the establishment period subsurface irrigation was cut on 24 Feb. 2006. Soil moisture content declined and reached a VWC below 3% on 5 Apr. 2006. A 54 days period of no-irrigation was followed by a re-growth period coupled with weekly biomass harvest during the second dry down cycle. Plants showed progressive wilting from 20 Mar. 2006 and severe necrosis after 12 Apr. 2006 (FIG. 19).

Chlorophyll Content in Different Lines

[0356]The chlorophyll content of leaves was measured with a chlorophyll meter (SPAD-502, Konica Minolta Sensing, Inc., Japan). For each plant, the second youngest, fully expanded leaf from three different tillers per plant was measured. Each data point in Table 3 represented the average of 12 measurements from the four clones of each transgenic line or wild type. The statistical significance levels of the difference in chlorophyll content between transgenic lines and wild type are shown: * significant difference at P<0.05; ** significant difference at P<0.01. The data indicate that three transgenic lines displayed higher chlorophyll contents than the wild type over the majority of time points: C4-20, D4-1 and D4-7 and that the chlorophyll content of D4-1 and D4-7 actually increased after the drought cycles (19 May 2006) as compared to pre-drought state (14 Feb. 2006).

TABLE-US-00017 TABLE 3 Different Levels Of Chlorophyll Contents In Non-Transgenic And Transgenic Lines Date WT C4-19 C4-20 D12-60 D12-61 D4-1 D4-7 02-14 40.2 40.8 49.5 ** 42.9 43.1 * 40.2 46.9 ** 03-09 47.9 47.5 54.0 * 49.8 ** 49.7 49.4 51.8 * 03-20 45.7 44.0 53.0 * 50.1 48.1 46.5 49.1 * 04-05 49.2 51.3 56.2 * 52.4 51.5 53.8 56.8 * 04-13 48.5 44.8 ** 59.0 ** 49.3 49.0 56.7 ** 58.1 ** 04-18 33.0 35.4 49.5 ** 32.0 34.5 49.4 ** 51.0 ** 04-20 34.0 32.6 41.0 ** 38.6 36.0 41.0 * 39.9 * 04-28 41.0 39.4 47.2 ** 45.1 * 41.6 45.3 ** 52.6 ** 05-05 45.1 43.8 50.7 * 47.1 44.8 46.8 51.6 * 05-12 44.2 45.0 49.9 ** 44.9 44.9 46.3 49.1 * 05-19 40.2 38.8 47.8 ** 37.3 34.4 43.7 47.5

Chlorophyll Fluorescence Parameters (ETR & Yield)

[0357]The chlorophyll fluorescence parameters, electron transport rate (ETR) and quantum yield (Yield), were measured with the PAM2000 fluorometer and are presented in FIGS. 20 and 21, respectively. The second fully expanded leaf was measured from three tillers per plant. Each data point in the figures represents the average of 12 measurements (3 measurements per clone, four clones per line) for each line. The volumetric water content of the soil is given in brackets in the figure legend. The absence of data for C4-20 during the second round of drought period indicates that the leaves of these transgenic plants were too small or narrow to be measured. During severe stress (VWC less than 3%) the wildtype did not show a statistically lower ETR or yield than the transgenic lines although there were statistically significant differences in the chlorophyll contents.

Above-Ground Biomass

[0358]All leaves were cut 2 days after re-watering at 2.5 cm clipping height. The fresh weights (FW) of leaves were measured immediately, then leaves were dried at 80° C. for 48 h and the dry weight (DW) was measured. The difference between fresh weight and dry weight was used as an indicator of early recovery from drought stress. The aboveground biomass in chamber 1 and 2 produced since during the first dry down cycle is shown in FIG. 22. Mass loss indicates the difference of fresh weight and dry weight over fresh weight. No transgenic line produced significantly more biomass than wildtype in the first dry down cycle. However, transgenic lines C4-19, C4-20, D4-1 and D12-60 exhibited comparatively less biomass loss.

[0359]The time course of the newly produced leaf biomass (DW) during the second dry down cycle with clipping intervals of 7 to 10 days are shown in FIG. 23. The bars indicate the SE of four readings from four replications of each line in one chamber. With decreasing of soil moisture, the newly produced biomass of each line decreased. Wild type line, which had the highest biomass before the beginning of the trial, always had the highest amount of DW among all lines at any clipping time, but the differences declined with progressive drought stress and there was no significant difference between wildtype and transgenic lines at the 2 Jun. 2006 harvest. This indicates that the loss in the ability to produce aboveground biomass in the transgenic plants is lower than wild type under drought stress conditions.

[0360]The above examples illustrate practice of the invention. It will be appreciated by those skilled in the art that numerous variations and modifications may be made without departing from the spirit and scope of the invention.

TABLE-US-00018 SUMMARY OF SEQUENCES SEQ ID NO: TYPE SPECIES REFERENCE 1 Polypeptide Plant Consensus 2 Polypeptide Dicotyledonous Consensus 3 Polypeptide Monocotyledonous Consensus 4 Polypeptide Lolium perenne ORF4 5 Polypeptide Oryza sativa BAC8588.1 6 Polypeptide Oryza sativa BAD03011.1 7 Polypeptide Oryza sativa BAD07720.1 8 Polypeptide Triticum aestivum BQ805537 9 Polypeptide Hordeum vulgare BQ466561 10 Polypeptide Oryza sativa CB683708 11 Polypeptide Triticum aestivum BJ236148 12 Polypeptide Triticum aestivum BE490521 13 Polypeptide Hordeum vulgare BQ468417 14 Polypeptide Triticum aestivum BJ292865 15 Polypeptide Oryza sativa CB632480 16 Polypeptide Triticum aestivum BJ282803 17 Polypeptide Sorghum bicolor CN123916 18 Polypeptide Sorghum bicolor CN139457 19 Polypeptide Sorghum bicolor CF757974 20 Polypeptide Zea mays CA830789 21 Polypeptide Sorghum bicolor CF757859 22 Polypeptide Zea mays AW120094 23 Polypeptide Saccharum officinarum CA186576 24 Polypeptide Saccharum officinarum CA135735 25 Polypeptide Sorghum bicolor CN123829 26 Polypeptide Triticum aestivum BJ299272 27 Polypeptide Triticum aestivum BJ312914 28 Polypeptide Triticum aestivum CK214681 29 Polypeptide Zea mays BG841716 30 Polypeptide Zea mays CD439661 31 Polypeptide Sorghum bicolor CN130285 32 Polypeptide Sorghum bicolor CN130210 33 Polypeptide Hordeum vulgare BJ450702 34 Polypeptide Sorghum bicolor BE601333 35 Polypeptide Zea mays CB350589 36 Polypeptide Zea mays BU092370 37 Polypeptide Arabidopsis thaliana NP 200279.1 38 Polypeptide Arabidopsis thaliana AAM64276.1 39 Polypeptide Arabidopsis thaliana NP 175518.1 40 Polypeptide Arabidopsis thaliana AAM10939.1 41 Polypeptide Arabidopsis thaliana NP 188962.2 42 Polypeptide Arabidopsis thaliana NP 567431.1 43 Polypeptide Arabidopsis thaliana NP 849383.1 44 Polypeptide Arabidopsis thaliana BAB01300.1 45 Polypeptide Arabidopsis thaliana NP 188620.1 46 Polypeptide Arabidopsis thaliana AAL91266.1 47 Polypeptide Arabidopsis thaliana NP 849566.1 48 Polypeptide Arabidopsis thaliana NP 195330.2 49 Polypeptide Arabidopsis thaliana T05498 50 Polypeptide Vitis vinifera CF518638 51 Polypeptide Citrus reticulata CF830716 52 Polypeptide Medicago trunculata BG647802 53 Polypeptide Vitis vinifera CF212640 54 Polypeptide Gossypium raimondii CO114007 55 Polypeptide Citrus reticulata CF830658 56 Polypeptide Solanum tuberosum CK273638 57 Polypeptide Solanum tuberosum CK274846 58 Polypeptide Solanum tuberosum CK272231 59 Polypeptide Vitis vinifera CF212555 60 Polypeptide Glycine max CF805866 61 Polypeptide Solanum tuberosum CK270277 62 Polypeptide Citrus reticulata CF830657 63 Polypeptide Thellungiella halophila BM985503 64 Polypeptide Populus tremuloides CF118919 65 Polypeptide Vitis vinifera CF512838 66 Polypeptide Brassica napus BQ704279 67 Polypeptide Vitis vinifera CF518705 68 Polypeptide Gossypium raimondii CO090106 69 Polypeptide Populus tremuloides CF118982 70 Polypeptide Prunus dulcis BU645447 71 Polypeptide Glycine max BI972959 72 Polypeptide Medicago trunculata CA918862 73 Polypeptide Medicago trunculata CA922356 74 Polypeptide Glycine max BQ253067 75 Polypeptide Vitis vinifera CD800120 76 Polypeptide Glycine soja BG043667 77 Polypeptide Solanum tuberosum CK259672 78 Polypeptide Gossypium raimondii CO079077 79 Polypeptide Vitis vinifera CF518625 80 Polypeptide Lycopersicon esculentum BG643869 81 Polypeptide Pinus taeda CO364588 82 Polynucleotide Lolium perenne ORF4 83 polynucleotide Oryza sativa AB110196 84 Polynucleotide Oryza sativa AP003876 85 Polynucleotide Oryza sativa AP004121 86 Polynucleotide Triticum aestivum BQ805537 87 Polynucleotide Hordeum vulgare BQ466561 88 Polynucleotide Oryza sativa CB683708 89 Polynucleotide Triticum aestivum BJ236148 90 Polynucleotide Triticum aestivum BE490521 91 Polynucleotide Hordeum vulgare BQ468417 92 Polynucleotide Triticum aestivum BJ292865 93 Polynucleotide Oryza sativa CB632480 94 Polynucleotide Triticum aestivum BJ282803 95 Polynucleotide Sorghum bicolor CN123916 96 Polynucleotide Sorghum bicolor CN139457 97 Polynucleotide Sorghum bicolor CF757974 98 Polynucleotide Zea mays CA830789 99 Polynucleotide Sorghum bicolor CF757859 100 Polynucleotide Zea mays AW120094 101 Polynucleotide Saccharum officinarum CA186576 102 Polynucleotide Saccharum officinarum CA135735 103 Polynucleotide Sorghum bicolor CN123829 104 Polynucleotide Triticum aestivum BJ299272 105 Polynucleotide Triticum aestivum BJ312914 106 Polynucleotide Triticum aestivum CK214681 107 Polynucleotide Zea mays BG841716 108 Polynucleotide Zea mays CD439661 109 Polynucleotide Sorghum bicolor CN130285 110 Polynucleotide Sorghum bicolor CN130210 111 Polynucleotide Hordeum vulgare BJ450702 112 Polynucleotide Sorghum bicolor BE601333 113 Polynucleotide Zea mays CB350589 114 Polynucleotide Zea mays BU092370 115 Polynucleotide Arabidopsis thaliana NM 124849 116 Polynucleotide Arabidopsis thaliana AY086197 117 Polynucleotide Arabidopsis thaliana NM 103985 118 Polynucleotide Arabidopsis thaliana AF488573 119 Polynucleotide Arabidopsis thaliana NM 113222 120 Polynucleotide Arabidopsis thaliana NM 117520 121 Polynucleotide Arabidopsis thaliana NM 179052 122 Polynucleotide Arabidopsis thaliana AB025631 123 Polynucleotide Arabidopsis thaliana NM 112876 124 Polynucleotide Arabidopsis thaliana AY090362 125 Polynucleotide Arabidopsis thaliana NM 179235 126 Polynucleotide Arabidopsis thaliana NM 119773 127 Polynucleotide Arabidopsis thaliana AL022373 128 Polynucleotide Vitis vinifera CF518638 129 Polynucleotide Citrus reticulate CF830716 130 Polynucleotide Medicago trunculata BG647802 131 Polynucleotide Vitis vinifera CF212640 132 Polynucleotide Gossypium raimondii CO114007 133 Polynucleotide Citrus reticulata CF830658 134 Polynucleotide Solanum tuberosum CK273638 135 Polynucleotide Solanum tuberosum CK274846 136 Polynucleotide Solanum tuberosum CK272231 137 Polynucleotide Vitis vinifera CF212555 138 Polynucleotide Glycine max CF805866 139 Polynucleotide Solanum tuberosum CK270277 140 Polynucleotide Citrus reticulata CF830657 141 Polynucleotide Thellungiella halophila BM985503 142 Polynucleotide Populus tremuloides CF118919 143 Polynucleotide Vitis vinifera CF512838 144 Polynucleotide Brassica napus BQ704279 145 Polynucleotide Vitis vinifera CF518705 146 Polynucleotide Gossypium raimondii CO090106 147 Polynucleotide Populus tremuloides CF118982 148 Polynucleotides Prunus dulcis BU645447 149 Polynucleotide Glycine max BI972959 150 Polynucleotide Medicago trunculata CA918862 151 Polynucleotide Medicago trunculata CA922356 152 Polynucleotide Glycine max BQ253067 153 Polynucleotide Vitis vinifera CD800120 154 Polynucleotide Glycine soja BG043667 155 Polynucleotide Solanum tuberosum CK259672 156 Polynucleotide Gossypium raimondii CO079077 157 Polynucleotide Vitis vinifera CF518625 158 Polynucleotide Lycopersicon esculentum BG643869 159 Polynucleotide Pinus taeda CO364588 160 Polypeptide Plant Consensus 161 Polypeptide Dicotyledonous Consensus 162 Polypeptide Monocotyledonous Consensus 163 Polypeptide Lolium perenne ORF12 164 Polypeptide Oryza sativa AA046041 165 Polypeptide Oryza sativa AAP42273.1 166 Polypeptide Triticum aestivum Q42430 167 Polypeptide Oryza sativa AAK01713 168 Polypeptide Oryza sativa BAC83752 169 Polypeptide Aegilops speltoides BQ840910 170 Polypeptide Secale cereale CD453233 171 Polypeptide Saccharum officinarum CA142551 172 Polypeptide Nicotiana Benthamiana AAQ54303 173 Polypeptide Capsicum annum AAQ10954 174 Polypeptide Medicago sativa CAB77055 175 Polypeptide Glycine max T09602 176 Polypeptide Arabidopsis thaliana AAF24959 177 Polypeptide Arabidopsis thaliana NP 174094 178 Polypeptide Datisca glomerata AAD26942 179 Polypeptide Arabidopsis thaliana CAA67229 180 Polypeptide Petunia X hybrida BAA05079 181 Polypeptide Arabidopsis thaliana NP 188952 182 Polypeptide Arabidopsis thaliana BAC43454 183 Polypeptide Arabidopsis thaliana NP 196054 184 Polypeptide Brassica rapa T14408 185 Polypeptide Brassica rapa T14409 186 Polypeptide Nicotiana tabacum T01985 187 Polypeptide Petunia X Hybrida BAA05077 188 Polypeptide Arabidopsis thaliana NP 199131 189 Polypeptide Arabidopsis thaliana AAM67193 190 Polypeptide Arabidopsis thaliana NP 190562 191 Polypeptide Petunia X hybrida BAA05076 192 Polypeptide Arabidopsis thaliana NP 201546 193 Polypeptide Solanum tuberosum CK267005 194 Polypeptide Gossypium raimondii CO122574 195 Polypeptide Vitis aestivalis CB074681 196 Polypeptide Lycopersicon esculentum BI421491 197 Polypeptide Medicago trunculata BI308195 198 Polypeptide Populus sp. BU884157 199 Polypeptide Citrus sinensis CK938508 200 Polypeptide Lotus corniculatus AP004523 201 Polynucleotide Lolium perenne ORF12 202 Polynucleotide Oryza sativa AY219847 203 Polynucleotide Oryza sativa AY289189 204 Polynucleotide Triticum aestivum D16415 205 Polynucleotide Oryza sativa AF332876 206 Polynucleotide Oryza sativa AP005149 207 Polynucleotide Aegilops speltoides BQ840910 208 Polynucleotide Secale cereale CD453233 209 Polynucleotide Saccarum officinarum CA142551 210 Polynucleotide Nicotiana benthamiana AY290702 211 Polynucleotide Capsicum annuum AF539746 212 Polynucleotide Medicago sativa Y18788 213 Polynucleotide Glycine max GMU68763 214 Polynucleotide Arabidopsis thaliana AC012375 215 Polynucleotide Arabidopsis thaliana NM 102538 216 Polynucleotide Datisca glomerata AF119050 217 Polynucleotide Arabidopsis thaliana X98671 218 Polynucleotide Petunia X hybrida D26086 219 Polynucleotide Arabidopsis thaliana NM 112848 220 Polynucleotide Arabidopsis thaliana AK118868 221 Polynucleotide Arabidopsis thaliana NM 120516 222 Polynucleotide Brassica rapa BRU76554 223 Polynucleotide Brassica rapa BRU76555 224 Polynucleotide Nicotiana tabacum AF053077 225 Polynucleotide Petunia X hybrida D26084 226 Polynucleotide Arabidopsis Thaliana NM 123683 227 Polynucleotide Arabidopsis thaliana AY088887 228 Polynucleotide Arabidopsis thaliana NM 114853 229 Polynucleotide Petunia x hybrida D26083 230 Polynucleotide Arabidopsis thaliana NM 126145 231 Polynucleotide Solanum tuberosum CK267005 232 Polynucleotide Gossypium raimondii CO122574 233 Polynucleotide Vitis aestivalis CB074681 234 Polynucleotide Lycopersicon esculentum BI421491 235 Polynucleotide Medicago trunculata BI308195 236 Polynucleotide Populus sp. BU884157 237 Polynucleotide Citrus sinensis CK938508 238 Polynucleotide Lotus corniculatus AP004523 239 Polynucleotide Lolium perenne Promoter 240 Polynucleotide -- Genetic construct ORF4 (FIG. 7/8) 241 Polynucleotide -- Genetic Construct ORF 12 (FIG. 9/10) 242 Polynucleotide -- Genetic construct pCORF 4

(FIG. 11/12) 243 Polynucleotide -- Genetic construct (FIG. 13/14) pCORF 12 244 Polypeptide -- Motif

Sequence CWU 1

247123PRTArtificialplant consensus 1Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa Xaa Lys Xaa Xaa Xaa Arg1 5 10 15Xaa Glu Lys Xaa Xaa Xaa Lys 20223PRTArtificialdicotyledonous consensus 2Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa Xaa Lys Xaa Xaa Leu Arg1 5 10 15Xaa Glu Lys Xaa Xaa Xaa Lys 20323PRTArtificialmonocotyledonous consensus 3Leu Xaa Xaa Xaa Ile Xaa Xaa Leu Lys Xaa Xaa Lys Xaa Glu Xaa Arg1 5 10 15Xaa Glu Lys Xaa Xaa Xaa Lys 204254PRTLolium perenne 4Met Ala Ser Pro Glu Gly Ala Asn Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Ala Ala Asp Phe Thr Ala Pro Pro Ala Gly Gly Phe 20 25 30Tyr Trp Ala Pro Pro Met Gln Pro Gln Met His Thr Gln Ala Pro Ala 35 40 45Val Ser Ala Thr Pro Pro Pro Asn His Cys Ala Glu Ile Asn Ser Pro 50 55 60Ile Ser Val Asp Trp Asp His Ala Lys Gly Gln Pro Thr Asn Lys Arg65 70 75 80Pro Arg Ser Glu Ser Gly Ala Gln Pro Ser Ser Lys Ala Cys Arg Glu 85 90 95Lys Ala Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala 100 105 110Val Leu Asp Pro Gly Lys Thr Pro Lys Ile Asp Lys Cys Ala Ile Leu 115 120 125Asn Asp Ala Ile Arg Ala Val Thr Glu Leu Arg Ser Glu Ala Glu Lys 130 135 140Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Lys Glu Leu Lys145 150 155 160Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Lys Leu Lys Ala Glu 165 170 175Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Met Asn Ala Arg Gln Ser 180 185 190Leu Val Pro His Leu Pro His Pro Ser Val Ile Pro Ala Ala Ala Phe 195 200 205Ala Ala Pro Gln Gly Gln Val Pro Gly Gln Lys Leu Met Met Pro Val 210 215 220Ile Gly Tyr His Gly Phe Pro Met Trp Gln Phe Met Pro Pro Ser Asp225 230 235 240Val Asp Thr Ser Asp Asp Pro Lys Ser Cys Pro Pro Val Ala 245 2505256PRTOryza sativa 5Met Ala Ser Pro Glu Gly Ser Thr Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Ala Ala Ala Gly Phe Asp Ala Ala Pro Ala Gly Gly 20 25 30Phe Tyr Trp Thr Thr Pro Ala Pro Pro Gln Ala Ala Leu Gln Pro Pro 35 40 45Pro Pro Gln Gln Gln Pro Val Ala Pro Ala Thr Ala Ala Pro Asn Ala 50 55 60Cys Ala Glu Ile Asn Gly Ser Val Asp Cys Glu His Gly Lys Glu Gln65 70 75 80Pro Thr Asn Lys Arg Pro Arg Ser Glu Ser Gly Thr Arg Pro Ser Ser 85 90 95Lys Ala Cys Arg Glu Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe 100 105 110Leu Glu Leu Gly Ala Val Leu Glu Pro Gly Lys Thr Pro Lys Met Asp 115 120 125Lys Ser Ser Ile Leu Asn Asp Ala Ile Arg Val Met Ala Glu Leu Arg 130 135 140Ser Glu Ala Gln Lys Leu Lys Glu Ser Asn Glu Ser Leu Gln Glu Lys145 150 155 160Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln 165 170 175Lys Leu Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Leu 180 185 190Asn Ala Arg Pro Ser Phe Val Pro His Pro Pro Val Ile Pro Ala Ser 195 200 205Ala Phe Thr Ala Pro Gln Gly Gln Ala Ala Gly Gln Lys Leu Met Met 210 215 220Pro Val Ile Gly Tyr Pro Gly Phe Pro Met Trp Gln Phe Met Pro Pro225 230 235 240Ser Asp Val Asp Thr Thr Asp Asp Thr Lys Ser Cys Pro Pro Val Ala 245 250 2556253PRTOryza sativa 6Met Ser Gly Thr Pro Ala Asp Gly Gly Gly Gly Gly Gly Gly Gly Gly1 5 10 15Gly Gly Ser Gly Asp Asp Trp Phe Leu Asp Cys Gly Ile Leu Glu Asp 20 25 30Leu Pro Ala Ala Ala Cys Gly Ala Phe Pro Trp Asp Ala Ser Pro Ser 35 40 45Cys Ser Asn Pro Ser Val Glu Val Ser Ser Tyr Val Asn Thr Thr Ser 50 55 60Tyr Val Leu Lys Glu Pro Gly Ser Asn Lys Arg Val Arg Ser Gly Ser65 70 75 80Cys Gly Arg Pro Thr Ser Lys Ala Ser Arg Glu Lys Ile Arg Arg Asp 85 90 95Lys Met Asn Asp Arg Phe Leu Glu Leu Gly Thr Thr Leu Glu Pro Gly 100 105 110Lys Pro Val Lys Ser Asp Lys Ala Ala Ile Leu Ser Asp Ala Thr Arg 115 120 125Met Val Ile Gln Leu Arg Ala Glu Ala Lys Gln Leu Lys Asp Thr Asn 130 135 140Glu Ser Leu Glu Asp Lys Ile Lys Glu Leu Lys Ala Glu Lys Asp Glu145 150 155 160Leu Arg Asp Glu Lys Gln Lys Leu Lys Val Glu Lys Glu Thr Leu Glu 165 170 175Gln Gln Val Lys Ile Leu Thr Ala Thr Pro Ala Tyr Met Pro His Pro 180 185 190Thr Leu Met Pro Ala Pro Tyr Pro Gln Ala Pro Leu Ala Pro Phe His 195 200 205His Ala Gln Gly Gln Ala Ala Gly Gln Lys Leu Met Met Pro Phe Val 210 215 220Gly Tyr Pro Gly Tyr Pro Met Trp Gln Phe Met Pro Pro Ser Glu Val225 230 235 240Asp Thr Ser Lys Asp Ser Glu Ala Cys Pro Pro Val Ala 245 2507236PRTOryza sativa 7Met Asp Gly Gly Gly Asp Pro Val Asp Glu Phe Leu Ile Gly Gly Gly1 5 10 15Gly Glu Asp Gly Asp Leu Gly Val Phe Cys Asp Gly Val Pro Thr Leu 20 25 30Pro Cys Asp Gly Gly Leu Gly Ile Asp Asp Val Ser Gly Asp Thr Cys 35 40 45Cys Leu Asp Gln Ser Val Leu Gly Lys Arg Gly Arg Asp Glu Ser Ser 50 55 60Ser Ser Gly Pro Lys Ser Lys Ala Cys Arg Glu Lys Ile Arg Arg Asp65 70 75 80Arg Leu Asn Asp Arg Phe Leu Glu Leu Ser Ser Val Ile Asn Pro Asp 85 90 95Lys Gln Ala Lys Leu Asp Lys Ala Asn Ile Leu Ser Asp Ala Ala Arg 100 105 110Leu Leu Ala Glu Leu Arg Gly Glu Ala Glu Lys Leu Lys Glu Ser Asn 115 120 125Glu Lys Leu Arg Glu Thr Ile Lys Asp Leu Lys Val Glu Lys Asn Glu 130 135 140Leu Arg Asp Glu Lys Val Thr Leu Lys Ala Glu Lys Glu Arg Leu Glu145 150 155 160Gln Gln Val Lys Ala Leu Ser Val Ala Pro Thr Gly Phe Val Pro His 165 170 175Leu Pro His Pro Ala Ala Phe His Pro Ala Ala Phe Pro Pro Phe Ile 180 185 190Pro Pro Tyr Gln Ala Leu Gly Asn Lys Asn Ala Pro Thr Pro Ala Ala 195 200 205Phe Gln Gly Met Ala Met Trp Gln Trp Leu Pro Pro Thr Ala Val Asp 210 215 220Thr Thr Gln Asp Pro Lys Leu Trp Pro Pro Asn Ala225 230 2358239PRTTriticum aestivum 8Cys Pro Leu Met Asp Asp Leu Ala Ala Ala Asp Phe Ala Ala Ala Ser1 5 10 15Ala Gly Gly Phe Tyr Trp Thr Pro Pro Met Gln Pro Gln Met His Thr 20 25 30Leu Ala Gln Ala Val Ser Ala Thr Pro Ala Pro Asn Pro Cys Ala Glu 35 40 45Ile Asn Ser Ser Val Ser Val Asp Trp Asp His Ala Lys Gly Gln Pro 50 55 60Lys Asn Lys Arg Pro Arg Ser Glu Thr Gly Ala Gln Pro Ser Ser Lys65 70 75 80Ala Cys Arg Glu Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu 85 90 95Glu Leu Gly Ala Val Leu Asp Pro Gly Lys Thr Pro Lys Ile Asp Lys 100 105 110Cys Ala Ile Leu Asn Asp Ala Ile Arg Ala Val Thr Glu Leu Arg Ser 115 120 125Glu Ala Gln Lys Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile 130 135 140Arg Glu Leu Lys Ala Asp Lys Asn Glu Leu Arg His Glu Lys Gln Lys145 150 155 160Met Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Met Asn 165 170 175Ala Arg Gln Ser Leu Val Pro His Pro Ser Val Ile Pro Ala Ala Ala 180 185 190Phe Ala Ala Ala Gln Gly Gln Ala Ala Gly His Lys Leu Met Met Pro 195 200 205Val Met Ser Tyr Pro Gly Phe Pro Met Trp Gln Phe Met Pro Pro Ser 210 215 220Asp Val Asp Thr Ser Asp Asp Pro Lys Ser Cys Pro Pro Val Ala225 230 2359205PRTHordeum vulgare 9Met Ala Ser Pro Glu Gly Ser Asn Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Ala Ala Asp Phe Ala Ala Val Pro Ala Gly Gly Phe 20 25 30Tyr Trp Asn Pro Pro Met Pro Pro Gln Met His Thr Leu Ala Gln Ala 35 40 45Val Ser Ala Thr Pro Ala Pro Asn Pro Cys Ala Glu Ile Asn Ser Ser 50 55 60Val Ser Val Asp Trp Asp His Ala Lys Gly Gln Pro Lys Asn Lys Arg65 70 75 80Pro Arg Ser Glu Thr Gly Ala Gln Pro Ser Ser Lys Ala Cys Arg Glu 85 90 95Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala 100 105 110Val Leu Asp Pro Gly Lys Thr Pro Lys Ile Asp Lys Cys Ala Ile Leu 115 120 125Asn Asp Ala Ile Arg Ala Val Thr Glu Leu Arg Ser Glu Ala Glu Lys 130 135 140Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Arg Glu Leu Lys145 150 155 160Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Lys Leu Lys Ala Glu 165 170 175Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Met Asn Ala Arg Gln Arg 180 185 190Leu Val Pro His Pro Ser Val Ile Pro Ala Thr Ala Phe 195 200 20510243PRTOryza sativa 10Met Ala Ser Pro Glu Gly Ser Thr Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Ala Ala Ala Gly Phe Asp Ala Ala Pro Ala Gly Gly 20 25 30Phe Tyr Trp Thr Thr Pro Ala Pro Pro Gln Ala Ala Leu Gln Pro Pro 35 40 45Pro Pro Gln Gln Gln Pro Val Ala Pro Ala Thr Ala Ala Pro Asn Ala 50 55 60Cys Ala Glu Ile Asn Gly Ser Val Asp Cys Glu His Gly Lys Glu Gln65 70 75 80Pro Thr Asn Lys Arg Pro Arg Ser Glu Ser Gly Thr Arg Pro Ser Ser 85 90 95Lys Ala Cys Arg Glu Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe 100 105 110Leu Glu Leu Gly Ala Val Leu Glu Pro Gly Lys Thr Pro Lys Met Asp 115 120 125Lys Ser Ser Ile Leu Asn Asp Ala Ile Arg Val Met Ala Glu Leu Arg 130 135 140Ser Glu Ala Gln Lys Leu Lys Glu Ser Asn Glu Ser Leu Gln Glu Lys145 150 155 160Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln 165 170 175Lys Leu Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Leu 180 185 190Asn Ala Arg Pro Ser Phe Val Pro His Pro Pro Val Ile Pro Ala Ser 195 200 205Ala Phe Thr Ala Pro Gln Gly Gln Ala Ala Gly Gln Lys Leu Met Met 210 215 220Pro Val Ile Gly Tyr Pro Gly Phe Pro Met Trp Gln Phe Met Pro Pro225 230 235 240Ser Asp Val11192PRTTriticum aestivum 11Met Ala Ser Pro Glu Gly Ser Asn Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Ala Ala Asp Phe Ala Ala Ala Ser Ala Gly Gly Phe 20 25 30Tyr Trp Thr Pro Pro Met Gln Pro Gln Met His Thr Leu Ala Gln Ala 35 40 45Val Ser Ala Thr Pro Ala Pro Asn Pro Cys Ala Glu Ile Asn Ser Ser 50 55 60Val Ser Val Asp Trp Asp His Ala Lys Gly Gln Pro Lys Asn Lys Arg65 70 75 80Pro Arg Ser Glu Thr Gly Ala Gln Pro Ser Ser Lys Ala Cys Arg Glu 85 90 95Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala 100 105 110Val Leu Asp Pro Gly Lys Thr Pro Lys Ile Asp Lys Cys Ala Ile Leu 115 120 125Asn Asp Ala Ile Arg Ala Val Thr Glu Leu Arg Ser Glu Ala Glu Lys 130 135 140Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Arg Glu Leu Lys145 150 155 160Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Lys Leu Lys Ala Glu 165 170 175Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Met Asn Ala Arg Gln Ser 180 185 19012191PRTTriticum aestivum 12Met Ala Ser Pro Glu Gly Ser Asn Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Ala Ala Asp Phe Ala Ala Ala Ser Thr Gly Gly Phe 20 25 30Tyr Trp Thr Pro Pro Met Gln Pro Gln Met His Thr Leu Ala Gln Ala 35 40 45Val Ser Ala Thr Pro Ala Pro Asn Pro Cys Ala Glu Ile Asn Ser Ser 50 55 60Val Ser Val Asp Trp Asp His Ala Lys Gly Gln Pro Lys Asn Lys Arg65 70 75 80Pro Arg Ser Glu Thr Gly Ala Gln Pro Ser Ser Lys Ala Cys Arg Glu 85 90 95Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala 100 105 110Val Leu Asp Pro Gly Lys Thr Pro Lys Ile Asp Lys Cys Ala Ile Leu 115 120 125Asn Asp Ala Ile Arg Ala Val Thr Glu Leu Arg Ser Glu Ala Glu Lys 130 135 140Leu Lys Asp Ser Asn Asp Ser Leu Gln Glu Lys Ile Arg Glu Leu Lys145 150 155 160Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Lys Leu Lys Ala Glu 165 170 175Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Met Asn Ala Arg Gln 180 185 19013190PRTHordeum vulgare 13Asn Ser Ser Val Ser Val Asp Trp Asp His Ala Lys Gly Gln Pro Lys1 5 10 15Asn Lys Arg Pro Arg Ser Glu Thr Gly Ala Gln Pro Ser Ser Lys Ala 20 25 30Cys Arg Glu Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu 35 40 45Leu Gly Ala Val Leu Asp Pro Gly Lys Thr Pro Lys Ile Asp Lys Cys 50 55 60Ala Ile Leu Asn Asp Ala Ile Arg Ala Val Thr Glu Leu Arg Ser Glu65 70 75 80Ala Glu Lys Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Arg 85 90 95Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Lys Leu 100 105 110Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Met Asn Ala 115 120 125Arg Gln Arg Leu Val Pro His Pro Ser Val Ile Pro Ala Thr Ala Phe 130 135 140Ala Ala Ala Gln Gly Gln Ala Ala Gly His Lys Leu Met Met Pro Val145 150 155 160Met Ser Tyr Pro Gly Phe Pro Met Trp Gln Phe Met Pro Pro Ser Asp 165 170 175Val Asp Thr Ser Asp Asp Pro Lys Ser Cys Pro Pro Val Ala 180 185 19014193PRTTriticum aestivummisc_feature(4)..(4)Xaa can be any naturally occurring amino acid 14Pro Ser Trp Xaa Asp Leu Ala Ala Ala Asp Phe Ala Ala Ala Ser Ala1 5 10 15Gly Gly Phe Tyr Trp Thr Pro Pro Met Gln Pro Gln Met His Thr Leu 20 25 30Ala Gln Ala Val Ser Ala Thr Pro Ala Pro Asn Pro Cys Ala Glu Ile 35 40 45Asn Ser Ser Val Ser Val Asp Trp Asp His Ala Lys Gly Gln Pro Lys 50 55 60Asn Lys Arg Pro Arg Ser Glu Thr Gly Ala Gln Pro Ser Ser Lys Ala65 70 75 80Cys Arg Glu Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu

85 90 95Leu Gly Ala Val Leu Asp Pro Gly Lys Thr Pro Lys Ile Asp Lys Cys 100 105 110Ala Ile Leu Asn Asp Ala Ile Arg Ala Val Thr Glu Leu Arg Ser Glu 115 120 125Ala Glu Lys Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Arg 130 135 140Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Lys Leu145 150 155 160Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Met Asn Ala 165 170 175Arg Gln Ser Leu Val Pro His Pro Ser Val Ile Pro Ala Ala Ala Phe 180 185 190Ala15191PRTOryza sativa 15Ala Glu Ile Asn Gly Ser Val Asp Cys Glu His Gly Lys Glu Gln Pro1 5 10 15Thr Asn Lys Arg Pro Arg Ser Glu Ser Gly Thr Arg Pro Ser Ser Lys 20 25 30Ala Cys Arg Glu Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu 35 40 45Glu Leu Gly Ala Val Leu Glu Pro Gly Lys Thr Pro Lys Met Asp Lys 50 55 60Ser Ser Ile Leu Asn Asp Ala Ile Arg Val Met Ala Glu Leu Arg Ser65 70 75 80Glu Ala Gln Lys Leu Lys Glu Ser Asn Glu Ser Leu Gln Glu Lys Ile 85 90 95Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Lys 100 105 110Leu Lys Ala Glu Lys Asp Ser Leu Glu Gln Gln Ile Lys Phe Leu Asn 115 120 125Ala Arg Pro Ser Phe Val Pro His Pro Pro Val Ile Pro Ala Ser Ala 130 135 140Phe Thr Ala Pro Gln Gly Gln Ala Ala Gly Gln Lys Leu Met Met Pro145 150 155 160Val Ile Gly Tyr Pro Gly Phe Pro Met Trp Gln Phe Met Pro Pro Ser 165 170 175Asp Val Asp Thr Thr Asp Asp Thr Lys Ser Cys Pro Pro Val Ala 180 185 19016170PRTTriticum aestivum 16Arg Ser Glu Thr Gly Ala Gln Pro Ser Ser Lys Ala Cys Arg Glu Lys1 5 10 15Val Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala Val 20 25 30Leu Asp Pro Gly Lys Thr Pro Lys Ile Asp Lys Cys Ala Ile Leu Asn 35 40 45Asp Ala Ile Arg Ala Val Thr Glu Leu Arg Ser Glu Ala Glu Lys Leu 50 55 60Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Arg Glu Leu Lys Ala65 70 75 80Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Lys Leu Lys Ala Glu Lys 85 90 95Glu Ser Leu Glu Gln Gln Ile Lys Phe Met Asn Ala Arg Gln Ser Leu 100 105 110Val Pro His Pro Ser Val Ile Pro Ala Ala Ala Phe Ala Ala Ala Gln 115 120 125Gly Gln Ala Ala Gly His Lys Leu Met Met Pro Val Met Ser Tyr Pro 130 135 140Gly Phe Pro Met Trp Gln Phe Met Pro Pro Ser Asp Val Asp Thr Ser145 150 155 160Asp Asp Pro Lys Ser Cys Pro Pro Val Ala 165 17017212PRTSorghum bicolormisc_feature(145)..(145)Xaa can be any naturally occurring amino acid 17Met Ala Ser Pro Glu Gly Thr Thr Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Val Ala Ala Asp Phe Ala Ala Ala Pro Ala Gly Gly 20 25 30Phe Phe Trp Ala Ala Pro Pro Ser Leu Gln Pro Gln Val Val Gln Ala 35 40 45Pro Val Gln Ser Val Val Ala Ala Ser Ala Pro Asn Pro Cys Val Glu 50 55 60Ile Ser Ser Ser Val Asp Cys Gly Gln Gly Lys Glu Gln Pro Thr Asn65 70 75 80Lys Arg Pro Arg Ser Glu Ser Thr Ala Glu Pro Ser Thr Lys Ala Ser 85 90 95Arg Glu Lys Ile Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu 100 105 110Gly Ala Ile Leu Glu Pro Gly Lys Thr Pro Lys Met Asp Lys Ser Ala 115 120 125Ile Leu Asn Asp Ala Ile Arg Val Val Gly Glu Leu Arg Ser Glu Ala 130 135 140Xaa Glu Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Lys Glu145 150 155 160Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys 165 170 175Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Leu Asn Ala Arg 180 185 190Pro Ser Leu Val Pro His His Pro Val Ile Ser Ala Ser Ala Phe Thr 195 200 205Ala Pro Gln Gly 21018185PRTSorghum bicolor 18Asp Cys Gly Gln Gly Lys Glu Gln Pro Thr Asn Lys Arg Pro Arg Ser1 5 10 15Glu Ser Thr Ala Glu Pro Ser Thr Lys Ala Ser Arg Glu Lys Ile Arg 20 25 30Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala Ile Leu Asp 35 40 45Pro Gly Lys Thr Pro Lys Met Asp Lys Ser Ala Ile Leu Asn Asp Ala 50 55 60Ile Arg Val Val Gly Glu Leu Arg Ser Glu Ala Lys Glu Phe Lys Asp65 70 75 80Ser Asn Glu Ser Leu Gln Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys 85 90 95Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu Ser 100 105 110Leu Glu Gln Gln Ile Lys Phe Leu Asn Ala Arg Pro Ser Leu Val Pro 115 120 125His His Pro Val Ile Ser Ala Ser Ala Phe Thr Ala Pro Gln Gly Pro 130 135 140Ala Val Ala Gly His Lys Leu Met Met Pro Val Leu Gly Tyr Pro Gly145 150 155 160Phe Pro Met Trp Gln Phe Met Pro Pro Ser Asp Val Asp Thr Ser Asp 165 170 175Asp Pro Lys Ser Cys Pro Pro Val Ala 180 18519205PRTSorghum bicolor 19Pro Gln Val Val Gln Ala Pro Val Gln Ser Val Val Ala Ala Ser Ala1 5 10 15Pro Asn Pro Cys Val Glu Ile Ser Ser Ser Val Asp Cys Gly Gln Gly 20 25 30Lys Glu Gln Pro Thr Asn Lys Arg Pro Arg Ser Glu Ser Thr Ala Glu 35 40 45Pro Ser Thr Lys Ala Ser Arg Glu Lys Ile Arg Arg Asp Lys Leu Asn 50 55 60Glu Arg Phe Leu Glu Leu Gly Ala Ile Leu Glu Pro Gly Lys Thr Pro65 70 75 80Lys Met Asp Lys Ser Ala Ile Leu Asn Asp Ala Ile Arg Val Val Gly 85 90 95Glu Leu Arg Ser Glu Ala Lys Glu Leu Lys Asp Ser Asn Glu Ser Leu 100 105 110Gln Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp 115 120 125Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile 130 135 140Lys Phe Leu Asn Ala Arg Pro Ser Leu Val Pro His His Pro Val Ile145 150 155 160Ser Ala Ser Ala Phe Thr Ala Pro Gln Gly Pro Ala Val Ala Gly His 165 170 175Lys Leu Met Met Pro Val Leu Gly Tyr Pro Gly Phe Pro Met Trp Gln 180 185 190Phe Met Pro Pro Ser Asp Val Asp Thr Ser Asp Asp Pro 195 200 20520197PRTZea mays 20Met Ala Ser Pro Glu Gly Thr Thr Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Val Ala Ala Asp Phe Ala Ala Ala Pro Ala Gly Gly 20 25 30Phe Phe Trp Ala Ala Pro Pro Ser Leu Gln Pro Gln Ala Pro Val Gln 35 40 45Ser Val Val Ala Ala Ser Ala Pro Asn Pro Cys Met Glu Ile Ser Ser 50 55 60Ser Val Asp Cys Gly Gln Glu Lys Glu Gln Pro Thr Asn Lys Arg Pro65 70 75 80Arg Ser Glu Ser Thr Thr Glu Ser Ser Thr Lys Ala Ser Arg Glu Lys 85 90 95Ile Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala Ile 100 105 110Leu Glu Pro Gly Lys Thr Pro Lys Met Asp Lys Thr Ala Ile Leu Ser 115 120 125Asp Ala Ile Arg Val Val Gly Glu Leu Arg Ser Glu Ala Lys Lys Leu 130 135 140Lys Asp Ser Asn Glu Asn Leu Gln Glu Lys Ile Lys Glu Leu Lys Ala145 150 155 160Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys 165 170 175Glu Ser Leu Glu Gln Gln Ile Lys Phe Leu Asn Ala Arg Pro Ser Leu 180 185 190Val Pro His His Pro 19521205PRTSorghum bicolor 21Pro Ser Leu Gln Pro Gln Val Val Gln Ala Pro Val Gln Ser Val Val1 5 10 15Ala Ala Ser Ala Pro Asn Pro Cys Val Glu Ile Ser Ser Ser Val Asp 20 25 30Cys Gly Gln Gly Lys Glu Gln Pro Thr Asn Lys Arg Pro Arg Ser Glu 35 40 45Ser Thr Ala Glu Pro Ser Thr Lys Ala Ser Arg Glu Lys Ile Arg Arg 50 55 60Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala Ile Leu Glu Pro65 70 75 80Gly Lys Thr Pro Lys Met Asp Lys Ser Ala Ile Leu Asn Asp Ala Ile 85 90 95Arg Val Val Gly Glu Leu Arg Ser Glu Ala Lys Glu Leu Lys Asp Ser 100 105 110Asn Glu Ser Leu Gln Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys Asn 115 120 125Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu Ser Leu 130 135 140Glu Gln Gln Ile Lys Phe Leu Asn Ala Arg Pro Ser Leu Val Pro His145 150 155 160His Pro Val Ile Ser Ala Ser Ala Phe Thr Ala Pro Gln Gly Pro Ala 165 170 175Val Ala Gly His Lys Leu Met Met Pro Val Leu Gly Tyr Pro Gly Phe 180 185 190Pro Met Trp Gln Phe Met Pro Pro Ser Asp Val Asp Thr 195 200 20522202PRTZea maysmisc_feature(192)..(192)Xaa can be any naturally occurring amino acid 22Ala Ala Asp Phe Ala Ala Ala Pro Ala Gly Gly Phe Phe Trp Ala Ala1 5 10 15Pro Pro Ser Leu Gln Pro Gln Ala Pro Val Gln Ser Val Val Ala Ala 20 25 30Ser Ala Pro Asn Pro Cys Met Glu Ile Ser Ser Ser Val Asp Cys Gly 35 40 45Gln Glu Lys Glu Gln Pro Thr Asn Lys Arg Pro Arg Ser Glu Ser Thr 50 55 60Thr Glu Ser Ser Thr Lys Ala Ser Arg Glu Lys Ile Arg Arg Asp Lys65 70 75 80Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala Ile Leu Glu Pro Gly Lys 85 90 95Thr Pro Lys Met Asp Lys Thr Ala Ile Leu Ser Asp Ala Ile Arg Val 100 105 110Val Gly Glu Leu Arg Ser Glu Ala Lys Lys Leu Lys Asp Ser Asn Glu 115 120 125Asn Leu Gln Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu 130 135 140Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu Ser Leu Glu Gln145 150 155 160Gln Ile Lys Phe Leu Asn Ala Arg Pro Ser Leu Val Pro His His Pro 165 170 175Val Ile Pro Ala Ser Ala Phe Pro Ala Pro Gln Gly Pro Ala Thr Xaa 180 185 190Ala Arg His Lys Leu Met Met Pro Val Ile 195 20023193PRTSaccharum officinarum 23Met Ala Ser Pro Glu Gly Thr Thr Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Val Ala Ala Asp Phe Ala Ala Ala Pro Ala Gly Gly 20 25 30Phe Phe Trp Ala Ala Pro Pro Ser Leu Gln Pro Gln Val Val Gln Ala 35 40 45Pro Val Gln Ser Val Val Ala Ala Ser Ala Pro Asn Pro Pro Cys Val 50 55 60Glu Ile Ser Ser Ser Val Asp Cys Gly Gln Gly Lys Glu Gln Pro Thr65 70 75 80Asn Lys Arg Pro Arg Ser Glu Ser Thr Ala Glu Pro Ser Thr Lys Ala 85 90 95Ser Arg Glu Lys Ile Arg Arg Asp Lys Leu Asn Lys Arg Phe Leu Glu 100 105 110Trp Gly Ala Ile Leu Glu Pro Gly Glu Thr Pro Lys Met Asp Lys Ser 115 120 125Ala Ile Leu Asn Asp Ala Ile Arg Ala Val Gly Glu Leu Arg Ser Glu 130 135 140Ala Lys Lys Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Lys145 150 155 160Glu Leu Lys Ala Glu Lys Asn Glu Ser Arg Asp Glu Lys Gln Arg Leu 165 170 175Lys Ala Glu Asn Glu Ser Leu Glu Gln Gln Ile Lys Phe Leu Asn Ala 180 185 190Arg24174PRTSaccharum officinarummisc_feature(166)..(166)Xaa can be any naturally occurring amino acid 24Glu Ile Ser Ser Ser Val Asp Cys Gly Gln Gly Lys Glu Gln Pro Thr1 5 10 15Asn Lys Arg Pro Arg Ser Glu Ser Thr Ala Glu Pro Ser Thr Lys Ala 20 25 30Ser Arg Glu Lys Ile Arg Arg Asp Lys Leu Asn Lys Arg Phe Leu Glu 35 40 45Leu Gly Ala Ile Leu Glu Pro Gly Glu Thr Pro Lys Met Asp Lys Ser 50 55 60Ala Ile Leu Asn Asp Ala Ile Arg Ala Val Gly Glu Leu Arg Ser Glu65 70 75 80Ala Lys Lys Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Lys 85 90 95Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu 100 105 110Lys Ala Glu Lys Glu Lys Pro Glu Gln Gln Ile Lys Phe Leu Asn Ala 115 120 125Arg Pro Ser Leu Val Pro His His Ser Val Ile Pro Ala Ser Ala Phe 130 135 140Ala Ala Pro Gln Gly Pro Ala Ala Ala Gly His Lys Leu Met Leu Pro145 150 155 160Val Leu Gly Tyr Pro Xaa Phe Pro Met Trp Gln Phe Met Pro 165 17025154PRTSorghum bicolor 25Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala Ile Leu Glu1 5 10 15Pro Gly Lys Thr Pro Lys Met Asp Lys Ser Ala Ile Leu Asn Asp Ala 20 25 30Ile Arg Val Val Gly Glu Leu Arg Ser Glu Ala Lys Glu Leu Lys Asp 35 40 45Ser Asn Glu Ser Leu Gln Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys 50 55 60Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu Ser65 70 75 80Leu Glu Gln Gln Ile Lys Phe Leu Asn Ala Arg Pro Ser Leu Val Pro 85 90 95His His Pro Ser Val Ile Ser Ala Ser Ala Phe Ile Ala Pro Gln Gly 100 105 110Pro Ala Val Ala Gly His Lys Leu Met Met Pro Val Leu Gly Tyr Pro 115 120 125Gly Phe Pro Met Trp Gln Phe Met Pro Pro Ser Asp Val Asp Thr Ser 130 135 140Asp Asp Pro Lys Ser Cys Pro Pro Val Ala145 15026128PRTTriticum aestivummisc_feature(5)..(5)Xaa can be any naturally occurring amino acid 26Lys Cys Ala Ile Xaa Asn Asp Ala Ile Arg Ala Val Thr Glu Leu Arg1 5 10 15Ser Glu Ala Glu Lys Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys 20 25 30Ile Arg Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln 35 40 45Lys Leu Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Met 50 55 60Asn Ala Arg Gln Ser Leu Val Pro His Pro Ser Val Ile Pro Ala Ala65 70 75 80Ala Phe Ala Ala Ala Gln Gly Gln Ala Ala Gly His Lys Leu Met Met 85 90 95Pro Val Met Ser Tyr Pro Gly Phe Pro Met Trp Gln Phe Met Pro Pro 100 105 110Ser Asp Val Asp Thr Ser Asp Asp Pro Lys Ser Cys Pro Pro Val Ala 115 120 12527188PRTTriticum aestivum 27Asp Leu Pro Lys Asp Ser Gly Ser Asn Lys Arg Leu Arg Ser Glu Pro1 5 10 15Cys Gly Arg Pro Thr Ser Lys Ala Cys Arg Glu Lys Val Arg Arg Asp 20 25 30Lys Leu Asn Asp Arg Phe Leu Glu Leu Gly Thr Thr Leu Asp Pro Gly 35 40 45Lys Pro Val Lys Ala Asp Lys Ala Ala Ile Leu Ser Asp Ala Thr Arg 50 55 60Met Val Thr Gln Leu Arg Ala Glu Ala Gln Gln Leu Lys Asp Thr Asn65 70 75 80Gly Ser Leu Glu Asp

Lys Ile Lys Glu Leu Lys Ala Glu Lys Asp Glu 85 90 95Leu Arg Asp Glu Lys Gln Lys Leu Lys Leu Glu Lys Glu Thr Leu Glu 100 105 110His Gln Met Lys Leu Leu Thr Ala Thr Pro Ala Tyr Met Pro His Pro 115 120 125Thr Met Met Pro Ser Pro Phe Ala Gln Ala Pro Met Ala Pro Phe His 130 135 140Ala Gln Gly Gln Ala Leu Gly Gln Lys Leu Met Met Pro Phe Val Gly145 150 155 160Tyr Pro Gly Tyr Pro Met Trp Gln Leu Met Pro Pro Ser Glu Val Asp 165 170 175Thr Ser Lys Asp Ser Glu Ala Cys Pro Pro Val Ala 180 18528232PRTTriticum aestivum 28Cys Asp Ser Trp Phe Leu Asp Cys Gly Ile Leu Lys Asp Leu Pro Ala1 5 10 15Ala Ala Trp Gly Val Phe Pro Gly Lys Ala Ser Phe Ser Trp Ser Asn 20 25 30Pro Ser Gly Glu Leu Gly Thr Gln Leu Thr Asn Leu Val Phe Pro Lys 35 40 45Asp Ser Gly Thr Asn Asn Arg Leu Ser Gln Ser Pro Phe Gly Arg Pro 50 55 60Thr Ser Lys Ala Cys Arg Glu Lys Val Arg Arg Asp Lys Leu Asn Asp65 70 75 80Arg Phe Leu Glu Leu Gly Thr Thr Leu Asp Pro Gly Lys Pro Val Lys 85 90 95Ala Asp Lys Ala Ala Ile Leu Ser Asp Ala Thr Arg Met Val Thr Gln 100 105 110Leu Arg Ala Glu Ala Gln Gln Leu Lys Asp Thr Asn Gly Ser Leu Glu 115 120 125Asp Lys Ile Lys Glu Leu Lys Ala Glu Lys Asp Glu Leu Arg Asp Glu 130 135 140Lys Gln Lys Leu Lys Leu Glu Lys Glu Thr Leu Glu His Gln Met Lys145 150 155 160Leu Leu Thr Ala Thr Pro Ala Tyr Met Pro His Pro Thr Met Met Pro 165 170 175Ser Pro Phe Ala Gln Ala Pro Met Ala Pro Phe His Ala Gln Gly Gln 180 185 190Ala Leu Gly Gln Lys Leu Met Met Pro Phe Val Gly Tyr Pro Gly Tyr 195 200 205Pro Met Trp Gln Leu Met Pro Pro Ser Glu Val Asp Thr Ser Lys Asp 210 215 220Ser Glu Ala Cys Pro Pro Val Ala225 23029144PRTZea maysmisc_feature(11)..(11)Xaa can be any naturally occurring amino acid 29Leu Gly Ala Ile Leu Glu Pro Gly Lys Thr Xaa Lys Met Asp Lys Thr1 5 10 15Ala Ile Leu Ser Asp Ala Ile Arg Val Val Gly Glu Leu Arg Ser Glu 20 25 30Ala Lys Lys Leu Lys Asp Ser Asn Glu Asn Leu Gln Glu Lys Ile Lys 35 40 45Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu 50 55 60Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Leu Asn Ala65 70 75 80Arg Pro Ser Leu Val Pro His His Pro Val Ile Pro Ala Ser Ala Phe 85 90 95Pro Ala Pro Gln Gly Pro Ala Ala Ala Ala Arg His Lys Leu Met Met 100 105 110Pro Val Ile Gly Tyr Pro Gly Phe Pro Met Trp Gln Phe Met Pro Pro 115 120 125Ser Asp Val Asp Thr Ser Asp Asp Pro Arg Ser Cys Pro Pro Val Ala 130 135 14030241PRTZea mays 30Gly Asp Asp Trp Phe Leu Asp Cys Gly Ile Leu Asp Asp Leu Pro Ala1 5 10 15Ala Ala Cys Gly Ala Phe Pro Trp Asp Ala Ser Pro Ser Ser Ser Asn 20 25 30Pro Ser Val Glu Val Gly Ser Tyr Val Asn Thr Asn Asp Val Phe Lys 35 40 45Glu Pro Asn Asp Val Phe Lys Glu Pro Gly Ser Asn Lys Arg Leu Arg 50 55 60Ser Gly Ser Asn Asp Val His Val Pro Thr Ser Lys Ala Ser Arg Glu65 70 75 80Lys Met Arg Arg Asn Lys Leu Asn Asp Arg Phe Leu Glu Leu Gly Ser 85 90 95Thr Leu Glu Pro Gly Lys Pro Val Lys Ala Asp Lys Ala Ala Ile Leu 100 105 110Ser Asp Ala Thr Arg Met Val Ile Gln Leu Arg Ser Glu Ala Gln Gln 115 120 125Leu Lys Glu Thr Asn Gly Ser Leu Glu Glu Lys Ile Lys Glu Leu Lys 130 135 140Ala Glu Lys Asp Glu Leu Arg Asp Glu Lys Gln Lys Leu Lys Leu Glu145 150 155 160Lys Glu Ser Leu Glu His Gln Met Lys Leu Met Thr Ser Thr Pro Thr 165 170 175Tyr Met Pro His Pro Thr Leu Met Pro Ala Pro Phe Pro Gln Ala Pro 180 185 190Leu Ala Pro Phe His Ala Gln Gly Gln Ala Ala Gly Gln Lys Leu Met 195 200 205Met Pro Phe Val Ser Tyr Pro Gly Tyr Pro Met Trp Gln Phe Met Pro 210 215 220Pro Ser Glu Val Asp Thr Ser Lys Asp Ser Glu Ala Cys Pro Pro Val225 230 235 240Ala31187PRTSorghum bicolor 31Asp Val Phe Lys Glu Pro Gly Ser Asn Lys Arg Leu Arg Ser Gly Ser1 5 10 15Asn Asp Val Pro Thr Ser Lys Ala Ser Arg Glu Lys Met Arg Arg Asn 20 25 30Lys Leu Asn Asp Arg Phe Leu Glu Leu Gly Ser Thr Leu Glu Pro Gly 35 40 45Lys Pro Val Lys Ala Asp Lys Ala Ala Ile Leu Ser Asp Ala Thr Arg 50 55 60Met Val Ile Gln Leu Arg Ser Glu Ala Gln Gln Leu Lys Glu Thr Asn65 70 75 80Gly Ser Leu Glu Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys Asp Glu 85 90 95Leu Arg Asp Glu Lys Gln Lys Leu Lys Leu Glu Lys Glu Ser Leu Glu 100 105 110His Gln Met Lys Leu Met Thr Ser Thr Pro Ala Tyr Met Pro His Pro 115 120 125Thr Leu Met Pro Ala Pro Phe Pro Gln Ala Pro Leu Ala Pro Phe His 130 135 140Ala Gln Gly Gln Ala Ala Gly Gln Lys Leu Met Met Pro Phe Val Ser145 150 155 160Tyr Pro Gly Tyr Pro Met Trp Gln Phe Met Pro Pro Ser Glu Val Asp 165 170 175Thr Ser Lys Asp Ser Glu Ala Cys Pro Pro Val 180 18532181PRTSorghum bicolor 32Ser Asn Lys Arg Leu Arg Ser Gly Ser Asn Asp Val Pro Thr Ser Lys1 5 10 15Ala Ser Arg Glu Lys Met Arg Arg Asn Lys Leu Asn Asp Arg Phe Leu 20 25 30Glu Leu Gly Ser Thr Leu Glu Pro Gly Lys Pro Val Lys Ala Asp Lys 35 40 45Ala Ala Ile Leu Ser Asp Ala Thr Arg Met Val Ile Gln Leu Arg Ser 50 55 60Glu Ala Gln Gln Leu Lys Glu Thr Asn Gly Ser Leu Glu Glu Lys Ile65 70 75 80Lys Glu Leu Lys Ala Glu Lys Asp Glu Leu Arg Asp Glu Lys Gln Lys 85 90 95Leu Lys Leu Glu Lys Glu Ser Leu Glu His Gln Met Lys Leu Met Thr 100 105 110Ser Thr Pro Ala Tyr Met Pro His Pro Thr Leu Met Pro Ala Pro Phe 115 120 125Pro Gln Ala Pro Leu Ala Pro Phe His Ala Gln Gly Gln Ala Ala Gly 130 135 140Gln Lys Leu Met Met Pro Phe Val Ser Tyr Pro Gly Tyr Pro Met Trp145 150 155 160Gln Phe Met Pro Pro Ser Glu Val Asp Thr Ser Lys Asp Ser Glu Ala 165 170 175Cys Pro Pro Val Ala 18033179PRTHordeum vulgaremisc_feature(5)..(5)Xaa can be any naturally occurring amino acid 33Lys Arg Leu Arg Xaa Gly Pro Cys Gly Arg Pro Thr Ser Lys Ala Cys1 5 10 15Arg Glu Lys Val Arg Arg Asp Lys Leu Asn Asp Arg Phe Leu Glu Leu 20 25 30Gly Thr Thr Leu Asp Pro Gly Lys Pro Val Lys Ala Asp Lys Ala Ala 35 40 45Ile Leu Ser Asp Ala Thr Arg Met Val Thr Gln Leu Arg Ala Glu Ala 50 55 60Lys Gln Leu Lys Asp Thr Asn Gly Ser Leu Glu Asp Lys Ile Lys Glu65 70 75 80Leu Lys Ala Glu Lys Asp Glu Leu Arg Asp Glu Lys Gln Lys Leu Lys 85 90 95Leu Glu Lys Glu Thr Leu Glu His Gln Met Lys Leu Leu Thr Ala Thr 100 105 110Pro Ala Tyr Met Pro His Pro Thr Met Met His Ser Pro Phe Ala Gln 115 120 125Ala Pro Met Ala Pro Phe His Ala Gln Gly His Ala Ser Ala Gln Lys 130 135 140Leu Met Met Pro Phe Val Gly Tyr Pro Gly Tyr Pro Met Trp Gln Leu145 150 155 160Met Pro Pro Ser Glu Val Asp Thr Ser Lys Asp Ser Glu Ala Cys Pro 165 170 175Pro Val Ala34127PRTSorghum bicolor 34Ala Ile Leu Asn Asp Ala Ile Arg Val Val Gly Glu Leu Arg Ser Glu1 5 10 15Ala Lys Glu Leu Lys Asp Ser Asn Glu Ser Leu Gln Glu Lys Ile Lys 20 25 30Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu 35 40 45Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Leu Asn Ala 50 55 60Arg Pro Ser Leu Val Pro His His Pro Val Ile Ser Ala Ser Ala Phe65 70 75 80Thr Ala Pro Gln Gly Pro Ala Val Ala Gly His Lys Leu Met Met Pro 85 90 95Val Leu Gly Tyr Pro Gly Phe Pro Met Trp Gln Phe Met Pro Pro Ser 100 105 110Asp Val Asp Thr Ser Asp Asp Pro Lys Ser Cys Pro Pro Val Ala 115 120 12535164PRTZea mays 35Cys Arg Glu Arg Met Arg Arg Asn Lys Leu Asn Asp Arg Phe Leu Glu1 5 10 15Leu Gly Ser Ala Leu Glu Pro Gly Lys Pro Val Lys Ala Asp Lys Ala 20 25 30Ala Ile Leu Ser Asp Ala Thr Arg Met Val Ile Gln Leu Arg Ser Glu 35 40 45Ser Gln Gln Leu Lys Glu Thr Asn Gly Ser Leu Glu Glu Lys Ile Lys 50 55 60Glu Leu Lys Ala Glu Lys Asp Glu Leu Arg Asp Glu Lys Gln Lys Leu65 70 75 80Lys Leu Glu Lys Glu Ser Leu Glu His Gln Met Lys Leu Met Ala Ser 85 90 95Ala Pro Ala Tyr Met Pro His Pro Thr Leu Met Pro Ala Pro Phe Ala 100 105 110Gln Ala Pro Leu Ala Pro Phe His Ala Gln Gly Gln Ala Ala Gly Gln 115 120 125Lys Leu Met Met Pro Phe Val Gly Tyr Pro Gly Tyr Pro Met Trp Gln 130 135 140Phe Met Pro Pro Ser Glu Val Asp Thr Ser Lys Asp Ser Glu Ala Cys145 150 155 160Pro Pro Val Ala36130PRTZea mays 36Lys Thr Ala Ile Leu Ser Asp Ala Ile Arg Val Val Gly Glu Leu Arg1 5 10 15Ser Glu Ala Lys Lys Leu Lys Asp Ser Asn Glu Asn Leu Gln Glu Lys 20 25 30Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln 35 40 45Arg Leu Lys Ala Glu Lys Glu Ser Leu Glu Gln Gln Ile Lys Phe Leu 50 55 60Asn Ala Arg Pro Ser Leu Val Pro His Arg Pro Val Ile Pro Ala Ser65 70 75 80Ala Phe Pro Ala Pro Gln Gly Pro Ala Ala Ala Ala Arg His Lys Leu 85 90 95Met Met Pro Val Ile Gly Tyr Pro Gly Phe Pro Met Trp Gln Phe Met 100 105 110Pro Pro Ser Asp Val Asp Thr Ser Asp Asp Pro Arg Ser Cys Pro Pro 115 120 125Val Ala 13037234PRTArabidopsis thaliana 37Met Val Ser Pro Glu Asn Ala Asn Trp Ile Cys Asp Leu Ile Asp Ala1 5 10 15Asp Tyr Gly Ser Phe Thr Ile Gln Gly Pro Gly Phe Ser Trp Pro Val 20 25 30Gln Gln Pro Ile Gly Val Ser Ser Asn Ser Ser Ala Gly Val Asp Gly 35 40 45Ser Ala Gly Asn Ser Glu Ala Ser Lys Glu Pro Gly Ser Lys Lys Arg 50 55 60Gly Arg Cys Glu Ser Ser Ser Ala Thr Ser Ser Lys Ala Cys Arg Glu65 70 75 80Lys Gln Arg Arg Asp Arg Leu Asn Asp Lys Phe Met Glu Leu Gly Ala 85 90 95Ile Leu Glu Pro Gly Asn Pro Pro Lys Thr Asp Lys Ala Ala Ile Leu 100 105 110Val Asp Ala Val Arg Met Val Thr Gln Leu Arg Gly Glu Ala Gln Lys 115 120 125Leu Lys Asp Ser Asn Ser Ser Leu Gln Asp Lys Ile Lys Glu Leu Lys 130 135 140Thr Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Thr Glu145 150 155 160Lys Glu Lys Leu Glu Gln Gln Leu Lys Ala Met Asn Ala Pro Gln Pro 165 170 175Ser Phe Phe Pro Ala Pro Pro Met Met Pro Thr Ala Phe Ala Ser Ala 180 185 190Gln Gly Gln Ala Pro Gly Asn Lys Met Val Pro Ile Ile Ser Tyr Pro 195 200 205Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala Ser Val Asp Thr Ser 210 215 220Gln Asp His Val Leu Arg Pro Pro Val Ala225 23038234PRTArabidopsis thaliana 38Met Val Ser Pro Glu Asn Ala Asn Trp Ile Cys Asp Leu Ile Asp Ala1 5 10 15Asp Tyr Gly Ser Phe Thr Ile Gln Gly Pro Gly Phe Ser Trp Pro Val 20 25 30Gln Gln Pro Ile Gly Val Ser Ser Asn Ser Ser Ala Gly Val Asp Gly 35 40 45Ser Ala Gly Asn Ser Glu Ala Ser Lys Glu Pro Gly Ser Lys Lys Arg 50 55 60Gly Arg Cys Glu Ser Ser Ser Ala Thr Ser Ser Lys Ala Cys Arg Glu65 70 75 80Lys Gln Arg Arg Asp Arg Leu Asn Asp Lys Phe Met Glu Leu Gly Ala 85 90 95Ile Leu Glu Pro Gly Asn Pro Pro Lys Thr Asp Lys Ala Ala Ile Leu 100 105 110Val Asp Ala Val Arg Met Val Thr Gln Leu Arg Gly Glu Ala Gln Lys 115 120 125Leu Lys Asp Ser Asn Ser Ser Leu Gln Asp Lys Ile Lys Glu Leu Lys 130 135 140Thr Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Thr Glu145 150 155 160Lys Glu Lys Leu Glu Gln Gln Leu Lys Ala Ile Asn Ala Pro Gln Pro 165 170 175Ser Phe Phe Pro Ala Pro Pro Met Met Pro Thr Ala Phe Ala Ser Ala 180 185 190Gln Gly Gln Ala Pro Gly Asn Lys Met Val Pro Ile Ile Ser Tyr Pro 195 200 205Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala Ser Val Asp Thr Ser 210 215 220Gln Asp His Val Leu Arg Pro Pro Val Ala225 23039226PRTArabidopsis thaliana 39Met Val Ser Pro Glu Asn Thr Asn Trp Leu Ser Asp Tyr Pro Leu Ile1 5 10 15Glu Gly Ala Phe Ser Asp Gln Asn Pro Thr Phe Pro Trp Gln Ile Asp 20 25 30Gly Ser Ala Thr Val Ser Val Glu Val Asp Gly Phe Leu Cys Asp Ala 35 40 45Asp Val Ile Lys Glu Pro Ser Ser Arg Lys Arg Ile Lys Thr Glu Ser 50 55 60Cys Thr Gly Ser Asn Ser Lys Ala Cys Arg Glu Lys Gln Arg Arg Asp65 70 75 80Arg Leu Asn Asp Lys Phe Thr Glu Leu Ser Ser Val Leu Glu Pro Gly 85 90 95Arg Thr Pro Lys Thr Asp Lys Val Ala Ile Ile Asn Asp Ala Ile Arg 100 105 110Met Val Asn Gln Ala Arg Asp Glu Ala Gln Lys Leu Lys Asp Leu Asn 115 120 125Ser Ser Leu Gln Glu Lys Ile Lys Glu Leu Lys Asp Glu Lys Asn Glu 130 135 140Leu Arg Asp Glu Lys Gln Lys Leu Lys Val Glu Lys Glu Arg Ile Asp145 150 155 160Gln Gln Leu Lys Ala Ile Lys Thr Gln Pro Gln Pro Gln Pro Cys Phe 165 170 175Leu Pro Asn Pro Gln Thr Leu Ser Gln Ala Gln Ala Pro Gly Ser Lys 180 185 190Leu Val Pro Phe Thr Thr Tyr Pro Gly Phe Ala Met Trp Gln Phe Met 195 200 205Pro Pro Ala Ala Val Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro 210 215 220Val Ala22540291PRTArabidopsis thaliana 40Met Gln Thr Asn Glu Asp Asn Ile Phe Gln Asp Phe Gly Ser Cys Gly1 5 10 15Val Asn Leu Met Gln Pro Gln Gln Glu Gln Phe Asp Ser Phe Asn Gly 20 25 30Asn Leu Glu Gln Val Cys Ser Ser Phe Arg Gly Gly Asn Asn Gly Val 35

40 45Val Tyr Ser Ser Ser Ile Gly Ser Ala Gln Leu Asp Leu Ala Ala Ser 50 55 60Phe Ser Gly Val Leu Gln Gln Glu Thr His Gln Val Cys Gly Phe Arg65 70 75 80Gly Gln Asn Asp Asp Ser Ala Val Pro His Leu Gln Gln Gln Gln Gly 85 90 95Gln Val Phe Ser Gly Val Val Glu Ile Asn Ser Ser Ser Ser Val Gly 100 105 110Ala Val Lys Glu Glu Phe Glu Glu Glu Cys Ser Gly Lys Arg Arg Arg 115 120 125Thr Gly Ser Cys Ser Lys Pro Gly Thr Lys Ala Cys Arg Glu Lys Leu 130 135 140Arg Arg Glu Lys Leu Asn Asp Lys Phe Met Asp Leu Ser Ser Val Leu145 150 155 160Glu Pro Gly Arg Thr Pro Lys Thr Asp Lys Ser Ala Ile Leu Asp Asp 165 170 175Ala Ile Arg Val Val Asn Gln Leu Arg Gly Glu Ala His Glu Leu Gln 180 185 190Glu Thr Asn Gln Lys Leu Leu Glu Glu Ile Lys Ser Leu Lys Ala Asp 195 200 205Lys Asn Glu Leu Arg Glu Glu Lys Leu Val Leu Lys Ala Glu Lys Glu 210 215 220Lys Met Glu Gln Gln Leu Lys Ser Met Val Val Pro Ser Pro Gly Phe225 230 235 240Met Pro Ser Gln His Pro Ala Ala Phe His Ser His Lys Met Ala Val 245 250 255Ala Tyr Pro Tyr Gly Tyr Tyr Pro Pro Asn Met Pro Met Trp Ser Pro 260 265 270Leu Pro Pro Ala Asp Arg Asp Thr Ser Arg Asp Leu Lys Asn Leu Pro 275 280 285Pro Val Ala 29041320PRTArabidopsis thaliana 41Met Tyr Pro Ser Ile Glu Asp Asp Asp Asp Leu Leu Ala Ala Leu Cys1 5 10 15Phe Asp Gln Ser Asn Gly Val Glu Asp Pro Tyr Gly Tyr Met Gln Thr 20 25 30Asn Glu Asp Asn Ile Phe Gln Asp Phe Gly Ser Cys Gly Val Asn Leu 35 40 45Met Gln Pro Gln Gln Glu Gln Phe Asp Ser Phe Asn Gly Asn Leu Glu 50 55 60Gln Val Cys Ser Ser Phe Arg Gly Gly Asn Asn Gly Val Val Tyr Ser65 70 75 80Ser Ser Ile Gly Ser Ala Gln Leu Asp Leu Ala Ala Ser Phe Ser Gly 85 90 95Val Leu Gln Gln Glu Thr His Gln Val Cys Gly Phe Arg Gly Gln Asn 100 105 110Asp Asp Ser Ala Val Pro His Leu Gln Gln Gln Gln Gly Gln Val Phe 115 120 125Ser Gly Val Val Glu Ile Asn Ser Ser Ser Ser Val Gly Ala Val Lys 130 135 140Glu Glu Phe Glu Glu Glu Cys Ser Gly Lys Arg Arg Arg Thr Gly Ser145 150 155 160Cys Ser Lys Pro Gly Thr Lys Ala Cys Arg Glu Lys Leu Arg Arg Glu 165 170 175Lys Leu Asn Asp Lys Phe Met Asp Leu Ser Ser Val Leu Glu Pro Gly 180 185 190Arg Thr Pro Lys Thr Asp Lys Ser Ala Ile Leu Asp Asp Ala Ile Arg 195 200 205Val Val Asn Gln Leu Arg Gly Glu Ala His Glu Leu Gln Glu Thr Asn 210 215 220Gln Lys Leu Leu Glu Glu Ile Lys Ser Leu Lys Ala Asp Lys Asn Glu225 230 235 240Leu Arg Glu Glu Lys Leu Val Leu Lys Ala Glu Lys Glu Lys Met Glu 245 250 255Gln Gln Leu Lys Ser Met Val Val Pro Ser Pro Gly Phe Met Pro Ser 260 265 270Gln His Pro Ala Ala Phe His Ser His Lys Met Ala Val Ala Tyr Pro 275 280 285Tyr Gly Tyr Tyr Pro Pro Asn Met Pro Met Trp Ser Pro Leu Pro Pro 290 295 300Ala Asp Arg Asp Thr Ser Arg Asp Leu Lys Asn Leu Pro Pro Val Ala305 310 315 32042283PRTArabidopsis thaliana 42Met Tyr Pro Ser Leu Asp Asp Asp Phe Val Ser Asp Leu Phe Cys Phe1 5 10 15Asp Gln Ser Asn Gly Ala Glu Leu Asp Asp Tyr Thr Gln Phe Gly Val 20 25 30Asn Leu Gln Thr Asp Gln Glu Asp Thr Phe Pro Asp Phe Val Ser Tyr 35 40 45Gly Val Asn Leu Gln Gln Glu Pro Asp Glu Val Phe Ser Ile Gly Ala 50 55 60Ser Gln Leu Asp Leu Ser Ser Tyr Asn Gly Val Leu Ser Leu Glu Pro65 70 75 80Glu Gln Val Gly Gln Gln Asp Cys Glu Val Val Gln Glu Glu Glu Val 85 90 95Glu Ile Asn Ser Gly Ser Ser Gly Gly Ala Val Lys Glu Glu Gln Glu 100 105 110His Leu Asp Asp Asp Cys Ser Arg Lys Arg Ala Arg Thr Gly Ser Cys 115 120 125Ser Arg Gly Gly Gly Thr Lys Ala Cys Arg Glu Arg Leu Arg Arg Glu 130 135 140Lys Leu Asn Glu Arg Phe Met Asp Leu Ser Ser Val Leu Glu Pro Gly145 150 155 160Arg Thr Pro Lys Thr Asp Lys Pro Ala Ile Leu Asp Asp Ala Ile Arg 165 170 175Ile Leu Asn Gln Leu Arg Asp Glu Ala Leu Lys Leu Glu Glu Thr Asn 180 185 190Gln Lys Leu Leu Glu Glu Ile Lys Ser Leu Lys Ala Glu Lys Asn Glu 195 200 205Leu Arg Glu Glu Lys Leu Val Leu Lys Ala Asp Lys Glu Lys Thr Glu 210 215 220Gln Gln Leu Lys Ser Met Thr Ala Pro Ser Ser Gly Phe Ile Pro His225 230 235 240Ile Pro Ala Ala Phe Asn His Asn Lys Met Ala Val Tyr Pro Ser Tyr 245 250 255Gly Tyr Met Pro Met Trp His Tyr Met Pro Gln Ser Val Arg Asp Thr 260 265 270Ser Arg Asp Gln Glu Leu Arg Pro Pro Ala Ala 275 28043277PRTArabidopsis thaliana 43Met Asp Val Asn Leu Phe Gly His Asp Asp Ser Cys Ser Asn Gly Ala1 5 10 15Glu Leu Asp Asp Tyr Thr Gln Phe Gly Val Asn Leu Gln Thr Asp Gln 20 25 30Glu Asp Thr Phe Pro Asp Phe Val Ser Tyr Gly Val Asn Leu Gln Gln 35 40 45Glu Pro Asp Glu Val Phe Ser Ile Gly Ala Ser Gln Leu Asp Leu Ser 50 55 60Ser Tyr Asn Gly Val Leu Ser Leu Glu Pro Glu Gln Val Gly Gln Gln65 70 75 80Asp Cys Glu Val Val Gln Glu Glu Glu Val Glu Ile Asn Ser Gly Ser 85 90 95Ser Gly Gly Ala Val Lys Glu Glu Gln Glu His Leu Asp Asp Asp Cys 100 105 110Ser Arg Lys Arg Ala Arg Thr Gly Ser Cys Ser Arg Gly Gly Gly Thr 115 120 125Lys Ala Cys Arg Glu Arg Leu Arg Arg Glu Lys Leu Asn Glu Arg Phe 130 135 140Met Asp Leu Ser Ser Val Leu Glu Pro Gly Arg Thr Pro Lys Thr Asp145 150 155 160Lys Pro Ala Ile Leu Asp Asp Ala Ile Arg Ile Leu Asn Gln Leu Arg 165 170 175Asp Glu Ala Leu Lys Leu Glu Glu Thr Asn Gln Lys Leu Leu Glu Glu 180 185 190Ile Lys Ser Leu Lys Ala Glu Lys Asn Glu Leu Arg Glu Glu Lys Leu 195 200 205Val Leu Lys Ala Asp Lys Glu Lys Thr Glu Gln Gln Leu Lys Ser Met 210 215 220Thr Ala Pro Ser Ser Gly Phe Ile Pro His Ile Pro Ala Ala Phe Asn225 230 235 240His Asn Lys Met Ala Val Tyr Pro Ser Tyr Gly Tyr Met Pro Met Trp 245 250 255His Tyr Met Pro Gln Ser Val Arg Asp Thr Ser Arg Asp Gln Glu Leu 260 265 270Arg Pro Pro Ala Ala 27544337PRTArabidopsis thaliana 44Met Gly Ile Arg Glu Asn Gly Ile Met Leu Val Ser Arg Glu Arg Glu1 5 10 15Arg Ala Arg Arg Leu Glu Asn Arg Glu Ser Ile Phe Ala Glu Pro Pro 20 25 30Cys Leu Leu Leu Ala His Arg Ile Ser Pro Ser Pro Ser Ile Leu Pro 35 40 45Ala Glu Glu Glu Val Met Asp Val Ser Ala Arg Lys Ser Gln Lys Ala 50 55 60Gly Arg Glu Lys Leu Arg Arg Glu Lys Leu Asn Glu His Phe Val Glu65 70 75 80Leu Gly Asn Val Leu Asp Pro Glu Arg Pro Lys Asn Asp Lys Ala Thr 85 90 95Ile Leu Thr Asp Thr Val Gln Leu Leu Lys Glu Leu Thr Ser Glu Val 100 105 110Asn Lys Leu Lys Ser Glu Tyr Thr Ala Leu Thr Asp Glu Ser Arg Glu 115 120 125Leu Thr Gln Glu Lys Asn Asp Leu Arg Glu Glu Lys Thr Ser Leu Lys 130 135 140Ser Asp Ile Glu Asn Leu Asn Leu Gln Tyr Gln Gln Arg Leu Arg Ser145 150 155 160Met Ser Pro Trp Gly Ala Ala Met Asp His Thr Val Met Met Ala Pro 165 170 175Pro Pro Ser Phe Pro Tyr Pro Met Pro Ile Ala Met Pro Pro Gly Ser 180 185 190Ile Pro Met His Pro Ser Met Pro Ser Tyr Thr Tyr Phe Gly Asn Gln 195 200 205Asn Pro Ser Met Ile Pro Ala Pro Cys Pro Thr Tyr Met Pro Tyr Met 210 215 220Pro Pro Asn Thr Val Val Glu Gln Gln Ser Val His Ile Pro Gln Asn225 230 235 240Pro Gly Asn Arg Ser Arg Glu Pro Arg Ala Lys Val Ser Arg Glu Ser 245 250 255Arg Ser Glu Lys Ala Glu Asp Ser Asn Glu Val Ala Thr Gln Leu Glu 260 265 270Leu Lys Thr Pro Gly Ser Thr Ser Asp Lys Asp Thr Leu Gln Arg Pro 275 280 285Glu Lys Thr Lys Arg Cys Lys Arg Asn Asn Asn Asn Asn Ser Ile Glu 290 295 300Glu Ser Ser His Ser Ser Lys Cys Ser Ser Ser Pro Ser Val Arg Asp305 310 315 320His Ser Ser Ser Ser Ser Val Ala Gly Gly Gln Lys Pro Asp Asp Ala 325 330 335Lys45284PRTArabidopsis thaliana 45Met Asp Val Ser Ala Arg Lys Ser Gln Lys Ala Gly Arg Glu Lys Leu1 5 10 15Arg Arg Glu Lys Leu Asn Glu His Phe Val Glu Leu Gly Asn Val Leu 20 25 30Asp Pro Glu Arg Pro Lys Asn Asp Lys Ala Thr Ile Leu Thr Asp Thr 35 40 45Val Gln Leu Leu Lys Glu Leu Thr Ser Glu Val Asn Lys Leu Lys Ser 50 55 60Glu Tyr Thr Ala Leu Thr Asp Glu Ser Arg Glu Leu Thr Gln Glu Lys65 70 75 80Asn Asp Leu Arg Glu Glu Lys Thr Ser Leu Lys Ser Asp Ile Glu Asn 85 90 95Leu Asn Leu Gln Tyr Gln Gln Arg Leu Arg Ser Met Ser Pro Trp Gly 100 105 110Ala Ala Met Asp His Thr Val Met Met Ala Pro Pro Pro Ser Phe Pro 115 120 125Tyr Pro Met Pro Ile Ala Met Pro Pro Gly Ser Ile Pro Met His Pro 130 135 140Ser Met Pro Ser Tyr Thr Tyr Phe Gly Asn Gln Asn Pro Ser Met Ile145 150 155 160Pro Ala Pro Cys Pro Thr Tyr Met Pro Tyr Met Pro Pro Asn Thr Val 165 170 175Val Glu Gln Gln Ser Val His Ile Pro Gln Asn Pro Gly Asn Arg Ser 180 185 190Arg Glu Pro Arg Ala Lys Val Ser Arg Glu Ser Arg Ser Glu Lys Ala 195 200 205Glu Asp Ser Asn Glu Val Ala Thr Gln Leu Glu Leu Lys Thr Pro Gly 210 215 220Ser Thr Ser Asp Lys Asp Thr Leu Gln Arg Pro Glu Lys Thr Lys Arg225 230 235 240Cys Lys Arg Asn Asn Asn Asn Asn Ser Ile Glu Glu Ser Ser His Ser 245 250 255Ser Lys Cys Ser Ser Ser Pro Ser Val Arg Asp His Ser Ser Ser Ser 260 265 270Ser Val Ala Gly Gly Gln Lys Pro Asp Asp Ala Lys 275 28046268PRTArabidopsis thaliana 46Met Ala Val Ser Cys Leu Phe Ile Val Ser Ser Asn Tyr Arg Gly Ala1 5 10 15Glu Met Val Val Glu Val Lys Lys Glu Ala Val Cys Ser Gln Lys Ala 20 25 30Glu Arg Glu Lys Leu Arg Arg Asp Lys Leu Lys Glu Gln Phe Leu Glu 35 40 45Leu Gly Asn Ala Leu Asp Pro Asn Arg Pro Lys Ser Asp Lys Ala Ser 50 55 60Val Leu Thr Asp Thr Ile Gln Met Leu Lys Asp Val Met Asn Gln Val65 70 75 80Asp Arg Leu Lys Ala Glu Tyr Glu Thr Leu Ser Gln Glu Ser Arg Glu 85 90 95Leu Ile Gln Glu Lys Ser Glu Leu Arg Glu Glu Lys Ala Thr Leu Lys 100 105 110Ser Asp Ile Glu Ile Leu Asn Ala Gln Tyr Gln His Gly Ile Lys Thr 115 120 125Met Val Pro Trp Val Pro His Tyr Ser Tyr His Ile Pro Phe Val Ala 130 135 140Ile Thr Gln Gly Gln Ser Ser Phe Ile Pro Tyr Ser Ala Ser Val Asn145 150 155 160Pro Leu Thr Glu Gln Gln Ala Ser Val Gln Gln His Ser Ser Ser Ser 165 170 175Ala Asp Ala Ser Met Lys Gln Asp Ser Lys Ile Lys Pro Leu Asp Leu 180 185 190Asp Leu Met Met Asn Ser Asn His Ser Gly Gln Gly Asn Asp Gln Lys 195 200 205Asp Asp Val Arg Leu Lys Leu Glu Leu Lys Ile His Ala Ser Ser Leu 210 215 220Ala Gln Gln Asp Val Ser Gly Lys Glu Lys Lys Val Ser Leu Thr Thr225 230 235 240Thr Ala Ser Ser Ser Asn Ser Tyr Ser Leu Ser Gln Ala Val Gln Asp 245 250 255Ser Ser Pro Gly Thr Val Asn Asp Met Leu Lys Pro 260 26547286PRTArabidopsis thaliana 47Met Asp Gln Pro Met Lys Pro Lys Thr Cys Ser Glu Ser Asp Phe Ala1 5 10 15Asp Asp Ser Ser Ala Ser Ser Ser Ser Ser Ser Gly Gln Asn Leu Arg 20 25 30Gly Ala Glu Met Val Val Glu Val Lys Lys Glu Ala Val Cys Ser Gln 35 40 45Lys Ala Glu Arg Glu Lys Leu Arg Arg Asp Lys Leu Lys Glu Gln Phe 50 55 60Leu Glu Leu Gly Asn Ala Leu Asp Pro Asn Arg Pro Lys Ser Asp Lys65 70 75 80Ala Ser Val Leu Thr Asp Thr Ile Gln Met Leu Lys Asp Val Met Asn 85 90 95Gln Val Asp Arg Leu Lys Ala Glu Tyr Glu Thr Leu Ser Gln Glu Ser 100 105 110Arg Glu Leu Ile Gln Glu Lys Ser Glu Leu Arg Glu Glu Lys Ala Thr 115 120 125Leu Lys Ser Asp Ile Glu Ile Leu Asn Ala Gln Tyr Gln His Arg Ile 130 135 140Lys Thr Met Val Pro Trp Val Pro His Tyr Ser Tyr His Ile Pro Phe145 150 155 160Val Ala Ile Thr Gln Gly Gln Ser Ser Phe Ile Pro Tyr Ser Ala Ser 165 170 175Val Asn Pro Leu Thr Glu Gln Gln Ala Ser Val Gln Gln His Ser Ser 180 185 190Ser Ser Ala Asp Ala Ser Met Lys Gln Asp Ser Lys Ile Lys Pro Leu 195 200 205Asp Leu Asp Leu Met Met Asn Ser Asn His Ser Gly Gln Gly Asn Asp 210 215 220Gln Lys Asp Asp Val Arg Leu Lys Leu Glu Leu Lys Ile His Ala Ser225 230 235 240Ser Leu Ala Gln Gln Asp Val Ser Gly Lys Glu Lys Lys Val Ser Leu 245 250 255Thr Thr Thr Ala Ser Ser Ser Asn Ser Tyr Ser Leu Ser Gln Ala Val 260 265 270Gln Asp Ser Ser Pro Gly Thr Val Asn Asp Met Leu Lys Pro 275 280 28548268PRTArabidopsis thaliana 48Met Ala Val Ser Cys Leu Phe Ile Val Ser Ser Asn Tyr Arg Gly Ala1 5 10 15Glu Met Val Val Glu Val Lys Lys Glu Ala Val Cys Ser Gln Lys Ala 20 25 30Glu Arg Glu Lys Leu Arg Arg Asp Lys Leu Lys Glu Gln Phe Leu Glu 35 40 45Leu Gly Asn Ala Leu Asp Pro Asn Arg Pro Lys Ser Asp Lys Ala Ser 50 55 60Val Leu Thr Asp Thr Ile Gln Met Leu Lys Asp Val Met Asn Gln Val65 70 75 80Asp Arg Leu Lys Ala Glu Tyr Glu Thr Leu Ser Gln Glu Ser Arg Glu 85 90 95Leu Ile Gln Glu Lys Ser Glu Leu Arg Glu Glu Lys Ala Thr Leu Lys 100 105 110Ser Asp Ile Glu Ile Leu Asn Ala Gln Tyr Gln His Arg Ile Lys Thr 115 120 125Met Val Pro Trp Val Pro His Tyr Ser Tyr His Ile Pro Phe Val Ala 130 135 140Ile Thr Gln

Gly Gln Ser Ser Phe Ile Pro Tyr Ser Ala Ser Val Asn145 150 155 160Pro Leu Thr Glu Gln Gln Ala Ser Val Gln Gln His Ser Ser Ser Ser 165 170 175Ala Asp Ala Ser Met Lys Gln Asp Ser Lys Ile Lys Pro Leu Asp Leu 180 185 190Asp Leu Met Met Asn Ser Asn His Ser Gly Gln Gly Asn Asp Gln Lys 195 200 205Asp Asp Val Arg Leu Lys Leu Glu Leu Lys Ile His Ala Ser Ser Leu 210 215 220Ala Gln Gln Asp Val Ser Gly Lys Glu Lys Lys Val Ser Leu Thr Thr225 230 235 240Thr Ala Ser Ser Ser Asn Ser Tyr Ser Leu Ser Gln Ala Val Gln Asp 245 250 255Ser Ser Pro Gly Thr Val Asn Asp Met Leu Lys Pro 260 26549272PRTArabidopsis thaliana 49Met Asp Gln Pro Met Lys Pro Lys Thr Cys Ser Glu Ser Asp Phe Ala1 5 10 15Asp Asp Ser Ser Ala Ser Ser Ser Ser Ser Ser Gly Gln Asn Leu Arg 20 25 30Gly Ala Glu Met Val Val Glu Val Lys Lys Glu Ala Val Cys Ser Gln 35 40 45Lys Ala Glu Arg Glu Lys Leu Arg Arg Asp Lys Leu Lys Glu Gln Phe 50 55 60Leu Glu Leu Gly Asn Ala Leu Asp Pro Asn Arg Pro Lys Ser Asp Lys65 70 75 80Ala Ser Val Leu Thr Asp Thr Ile Gln Met Leu Lys Asp Val Met Asn 85 90 95Gln Val Asp Arg Leu Lys Ala Glu Tyr Glu Thr Leu Ser Gln Glu Ser 100 105 110Arg Glu Leu Ile Gln Glu Lys Ser Glu Leu Arg Glu Glu Lys Ala Thr 115 120 125Leu Lys Ser Asp Ile Glu Ile Leu Asn Ala Gln Tyr Gln His Arg Ile 130 135 140Lys Thr Met Val Pro Trp Gly Gln Ser Ser Phe Ile Pro Tyr Ser Ala145 150 155 160Ser Val Asn Pro Leu Thr Glu Gln Gln Ala Ser Val Gln Gln His Ser 165 170 175Ser Ser Ser Ala Asp Ala Ser Met Lys Gln Asp Ser Lys Ile Lys Pro 180 185 190Leu Asp Leu Asp Leu Met Met Asn Ser Asn His Ser Gly Gln Gly Asn 195 200 205Asp Gln Lys Asp Asp Val Arg Leu Lys Leu Glu Leu Lys Ile His Ala 210 215 220Ser Ser Leu Ala Gln Gln Val Ser Asp Leu Phe Asn Ser Phe Ala Asn225 230 235 240Lys Leu Phe His Gly Leu Thr Arg Val Tyr Phe His Ala Gly Cys Phe 245 250 255Trp Lys Arg Glu Glu Ser Lys Leu Asp Asn His Cys Lys Leu Ile Glu 260 265 27050237PRTVitis vinifera 50Met Val Ser Pro Glu Ala Thr Asn Trp Leu Tyr Glu Tyr Gly Leu Ile1 5 10 15Glu Asp Ile Pro Val Pro Asp Ser Asn Phe Ala Asn Thr Asn Ser Gly 20 25 30Phe Ala Trp Thr Pro Val Gln Ala Leu Asn Thr Ser Ala Asn Val Ser 35 40 45Gly Glu Ile Asp Gly Ser Phe Gly Asp Ser Asp Gly Ile Lys Glu Thr 50 55 60Gly Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Gly Ala Ser Ser Ser65 70 75 80Lys Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe 85 90 95Met Glu Leu Gly Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp 100 105 110Lys Ser Ser Ile Leu Ile Asp Ala Val Arg Met Val Thr Gln Leu Arg 115 120 125Gly Glu Ser Gln Lys Leu Lys Asp Ser Asn Ser Ser Leu Gln Glu Lys 130 135 140Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln145 150 155 160Arg Leu Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Ala Met 165 170 175Asn Ala Gln Pro Ser Phe Leu Pro Pro Val Pro Ser Ile Pro Ala Ala 180 185 190Phe Ala Ala Gln Gly Gln Ala Gly Gly Asp Lys Leu Val Pro Phe Ile 195 200 205Gly Tyr Pro Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala Ala Val 210 215 220Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro Val Ala225 230 23551232PRTCitrus reticulata 51Glu Asn Thr Asn Trp Leu Leu Asp Tyr Pro Leu Ile Asp Asp Ile Thr1 5 10 15Val Pro Asp Gly Asn Phe Ser Val Ser Ala Ser Gly Phe Thr Trp Thr 20 25 30Val Gln Pro Pro Ile Asn Gly Pro Ser Asn Gly Cys Val Glu Ile Asp 35 40 45Ser Ala Phe Gly Asp Ser Asn Gly Leu Lys Glu Ser Ser Lys Lys Arg 50 55 60Val Arg Ser Glu Ser Cys Gly Ser Ser Ser Ser Lys Ala Cys Arg Glu65 70 75 80Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe Val Glu Leu Ala Ser 85 90 95Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ala Ala Ile Leu 100 105 110Ile Asp Ala Val Arg Met Val Thr Gln Leu Arg Ser Glu Ala Gln Lys 115 120 125Leu Lys Asp Ser Asn Ser Ser Leu Gln Glu Lys Ile Lys Glu Leu Lys 130 135 140Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu145 150 155 160Lys Glu Lys Ile Glu Gln Gln Leu Lys Ala Met Ser Thr Gln Pro Ser 165 170 175Phe Leu Thr Pro Pro Pro Ala Ile Pro Ala Ala Phe Ala Ala Gln Gly 180 185 190Gln Ala Pro Gly Asn Lys Leu Met Pro Phe Ile Ser Tyr Pro Gly Val 195 200 205Ala Met Trp Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser Gln Asp 210 215 220His Val Leu Arg Pro Pro Val Ala225 23052230PRTMedicago truncatula 52Met Val Ser Pro Glu Asn Thr Asn Trp Leu Phe Asp Tyr Pro Leu Ile1 5 10 15Asp Glu Ile Pro Val Ser Val Asp Gly Ser Phe Ala Phe Thr Trp Pro 20 25 30Pro Pro His Leu Ser Asn Gly Gly Ile Glu Met Asp Asp Ser Ser Leu 35 40 45Val Asp Ser Asp Gly Ile Lys Glu Pro Gly Ser Lys Lys Arg Gly Arg 50 55 60Ser Asp Ser Cys Ala Pro Ser Ser Ser Lys Ala Cys Arg Glu Lys Leu65 70 75 80Arg Arg Asp Arg Leu Asn Asp Lys Phe Val Glu Leu Gly Ser Ile Leu 85 90 95Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ala Ala Ile Leu Ile Asp 100 105 110Ala Val Arg Met Val Thr Gln Leu Arg Gly Glu Ala Gln Lys Leu Lys 115 120 125Asp Ser Asn Ser Arg Leu Gln Glu Lys Ile Lys Glu Leu Lys Val Glu 130 135 140Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu145 150 155 160Lys Leu Glu Gln Gln Val Lys Ser Met Asn Thr Gln Pro Gly Phe Leu 165 170 175Thr His Pro Pro Ala Ile Pro Ala Ala Phe Ala His Gln Gly Gln Ala 180 185 190Pro Ser Asn Lys Leu Met Pro Phe Met Ser Tyr Pro Gly Val Ala Met 195 200 205Trp Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser Gln Asp His Val 210 215 220Leu Arg Pro Pro Val Ala225 23053229PRTVitis vinifera 53Met Val Ser Pro Glu Ala Thr Asn Trp Leu Tyr Glu Tyr Gly Leu Ile1 5 10 15Glu Asp Ile Pro Val Pro Asp Ser Asn Phe Ala Asn Thr Asn Ser Gly 20 25 30Phe Ala Trp Thr Pro Val Gln Ala Leu Asn Thr Ser Ala Asn Val Ser 35 40 45Gly Glu Ile Asp Gly Ser Phe Gly Asp Ser Asp Gly Ile Lys Glu Thr 50 55 60Gly Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Gly Ala Ser Ser Ser65 70 75 80Lys Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe 85 90 95Met Glu Leu Gly Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp 100 105 110Lys Ser Ser Ile Leu Ile Asp Ala Val Arg Met Val Thr Gln Leu Arg 115 120 125Gly Glu Ser Gln Lys Leu Lys Asp Ser Asn Ser Ser Leu Gln Glu Lys 130 135 140Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln145 150 155 160Arg Leu Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Ala Met 165 170 175Asn Ala Gln Pro Ser Phe Leu Pro Pro Val Pro Ser Ile Pro Ala Ala 180 185 190Phe Ala Ala Gln Gly Gln Ala Gly Gly Asn Lys Leu Val Pro Phe Ile 195 200 205Gly Tyr Pro Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala Ala Val 210 215 220Asp Thr Leu Thr Asp22554238PRTGossypium raimondii 54Met Val Ser Pro Glu Asn Phe Asn Tyr Trp Ser His Phe Asp Tyr Ala1 5 10 15Thr Leu Ile His Asp Ile Pro Val Pro Asp Asp Pro Tyr Ala Gly Phe 20 25 30Ala Trp Ser Thr Gln Pro Ile Asp Ala Pro Ser Asn Val Val Ser Val 35 40 45Glu Ile Asp Gly Ser Phe Gly Asp Ser Asp Gly Leu Lys Glu Ser Gly 50 55 60Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Asn Ala Ser Ser Ser Lys65 70 75 80Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe Met 85 90 95Glu Leu Gly Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys 100 105 110Ser Ala Ile Leu Ile Asp Ala Val Arg Met Val Thr Gln Leu Arg Gly 115 120 125Glu Ala Gln Lys Leu Lys Asp Ser Asn Thr Ser Leu Gln Glu Arg Ile 130 135 140Lys Glu Leu Lys Ser Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg145 150 155 160Leu Lys Ala Glu Lys Glu Arg Leu Glu Gln Gln Leu Lys Ala Met Asn 165 170 175Ala Gln Pro Ser Phe Met Pro Pro Ala Pro Pro Ala Ile Pro Ala Ala 180 185 190Phe Ala Ala Ala Pro Gly Gln Ala Pro Gly Asn Lys Leu Val Pro Leu 195 200 205Ile Gly Tyr Pro Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala Ala 210 215 220Val Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro Val Ala225 230 23555220PRTCitrus reticulata 55Met Val Ser Pro Glu Asn Thr Asn Trp Leu Leu Asp Tyr Pro Leu Ile1 5 10 15Asp Asp Ile Thr Val Pro Asp Gly Asn Phe Ser Val Ser Ala Ser Gly 20 25 30Phe Thr Trp Thr Val Gln Pro Pro Ile Asn Gly Pro Ser Asn Gly Cys 35 40 45Val Glu Ile Asp Ser Ala Phe Gly Asp Ser Asn Gly Leu Lys Glu Ser 50 55 60Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Gly Ser Ser Ser Ser Lys65 70 75 80Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe Val 85 90 95Glu Leu Ala Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys 100 105 110Ala Ala Ile Leu Ile Asp Ala Val Arg Met Val Thr Gln Leu Arg Ser 115 120 125Glu Ala Gln Lys Leu Lys Asp Ser Asn Ser Ser Leu Gln Glu Lys Ile 130 135 140Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg145 150 155 160Leu Lys Ala Glu Lys Glu Lys Ile Glu Gln Gln Leu Lys Ala Met Ser 165 170 175Thr Gln Pro Ser Phe Leu Thr Pro Pro Ala Ile Pro Ala Ala Phe Ala 180 185 190Ala Gln Gly Gln Ala Pro Gly Asn Lys Leu Met Pro Phe Ile Ser Tyr 195 200 205Pro Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala 210 215 22056233PRTSolanum tuberosum 56Met Val Ser Pro Glu Asn Thr Asn Trp Leu Tyr Asp Tyr Gly Phe Glu1 5 10 15Asp Ser Ser Val Pro Asp Ser Asn Phe Ser Pro Ser Ala Ser Gly Phe 20 25 30Asn Trp Pro Val Gln Asn Leu Asn Gly Ser Arg Asn Val Ser Ser Glu 35 40 45Ile Asp Gly Ser Ile Gly Glu Ser Asp Tyr Pro Lys Glu Ser Gly Ser 50 55 60Lys Lys Arg Ala Arg Val Glu Ser Cys Ala Pro Thr Ser Ser Lys Ala65 70 75 80Cys Arg Glu Lys Leu Arg Arg Asp Lys Leu Asn Asp Lys Phe Met Glu 85 90 95Leu Gly Ala Leu Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ser 100 105 110Ala Ile Leu Val Asp Ala Val Arg Met Val Thr Gln Leu Arg Asp Glu 115 120 125Ala Gln Lys Leu Lys Asp Ser Asn Leu Asn Leu Gln Glu Lys Ile Lys 130 135 140Glu Leu Lys Val Glu Lys Thr Glu Leu Arg Asp Glu Lys Gln Arg Leu145 150 155 160Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Thr Thr Ser Ala 165 170 175Gln Pro Ser Phe Leu Pro Pro Ala Ile Pro Ser Ala Phe Ala Ala His 180 185 190Gly Gln Phe Pro Gly Ser Lys Leu Val Pro Ile Met Ser Tyr Pro Gly 195 200 205Val Ala Met Trp Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser Gln 210 215 220Asp His Val Leu Arg Pro Pro Val Ala225 23057233PRTSolanum tuberosum 57Met Val Ser Pro Glu Asn Thr Asn Trp Leu Tyr Asp Tyr Gly Phe Glu1 5 10 15Asp Ser Ser Val Pro Asp Ser Asn Phe Ser Ala Ser Ala Ser Gly Phe 20 25 30Asn Trp Pro Val Gln Asn Leu Asn Gly Ser Arg Asn Val Ser Ser Glu 35 40 45Ile Asp Gly Ser Ile Gly Glu Ser Asp Tyr Pro Lys Glu Ser Gly Ser 50 55 60Lys Lys Arg Ala Arg Val Glu Ser Cys Ala Pro Thr Ser Ser Lys Ala65 70 75 80Cys Arg Glu Lys Leu Arg Arg Asp Lys Leu Asn Asp Lys Phe Met Glu 85 90 95Leu Gly Ala Leu Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ser 100 105 110Ala Ile Leu Val Asp Ala Val Arg Met Val Thr Gln Leu Arg Asp Glu 115 120 125Ala Gln Lys Leu Lys Asp Ser Asn Leu Asn Leu Gln Glu Lys Ile Lys 130 135 140Glu Leu Lys Val Glu Lys Thr Glu Leu Arg Asp Glu Lys Gln Arg Leu145 150 155 160Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Thr Thr Ser Ala 165 170 175Gln Pro Ser Phe Leu Pro Pro Ala Val Pro Ser Ala Phe Ala Ala His 180 185 190Gly Gln Phe Pro Gly Ser Lys Leu Val Pro Ile Met Ser Tyr Pro Gly 195 200 205Val Ala Met Trp Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser Gln 210 215 220Asp His Val Leu Arg Pro Pro Val Ala225 23058233PRTSolanum tuberosum 58Met Val Ser Pro Glu Asn Thr Asn Trp Leu Tyr Asp Tyr Gly Phe Glu1 5 10 15Asp Ser Ser Val Pro Asp Ser Asn Phe Ser Ala Ser Ala Ser Gly Phe 20 25 30Asn Trp Pro Val Gln Asn Leu Asn Gly Ser Arg Asn Val Ser Ser Glu 35 40 45Ile Asp Gly Ser Ile Gly Glu Ser Asp Tyr Pro Lys Glu Ser Gly Ser 50 55 60Lys Lys Arg Ala Arg Val Glu Ser Cys Ala Pro Thr Ser Ser Lys Ala65 70 75 80Cys Arg Glu Lys Leu Arg Arg Asp Lys Leu Asn Asp Lys Phe Met Glu 85 90 95Leu Gly Ala Leu Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ser 100 105 110Ala Ile Leu Val Asp Ala Val Arg Met Val Thr Gln Leu Arg Asp Glu 115 120 125Ala Gln Lys Leu Lys Asp Ser Asn Leu Asn Leu Gln Glu Lys Ile Lys 130 135 140Glu Leu Lys Val Glu Lys Thr Glu Leu Arg Asp Glu Lys Gln Arg Leu145 150 155 160Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Thr Thr Ser Ala 165 170 175Gln Pro Ser Phe Leu Pro Pro Ala Ile Pro Ser Ala Phe Ala Ala His 180 185 190Gly Gln Phe Pro Gly Ser Lys Leu Val Pro Ile Met Ser Tyr Pro Gly 195 200 205Val Ala Met Trp Gln Phe Met Pro Pro

Ala Ala Val Asp Thr Ser Gln 210 215 220Asp His Val Leu Arg Pro Pro Val Ala225 23059223PRTVitis vinifera 59Leu Ile Glu Asp Ile Pro Val Pro Asp Ser Asn Phe Ala Asn Thr Asn1 5 10 15Ser Gly Phe Ala Trp Thr Pro Val Gln Ala Leu Asn Thr Ser Ala Asn 20 25 30Val Ser Gly Glu Ile Asp Gly Ser Phe Gly Asp Ser Asp Gly Ile Lys 35 40 45Glu Thr Gly Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Gly Ala Ser 50 55 60Ser Ser Lys Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp65 70 75 80Lys Phe Met Glu Leu Gly Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys 85 90 95Thr Asp Lys Ser Ser Ile Leu Ile Asp Ala Val Arg Met Val Thr Gln 100 105 110Leu Arg Gly Glu Ser Gln Lys Leu Lys Asp Ser Asn Ser Ser Leu Gln 115 120 125Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu 130 135 140Lys Gln Arg Leu Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys145 150 155 160Ala Met Asn Ala Gln Pro Ser Phe Leu Pro Pro Val Pro Ser Ile Pro 165 170 175Ala Ala Phe Ala Ala Gln Gly Gln Ala Gly Gly Asn Lys Leu Val Pro 180 185 190Phe Ile Gly Tyr Pro Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala 195 200 205Ala Val Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro Val Ala 210 215 22060214PRTGlycine max 60Glu Asn Ser Asn Trp Leu Phe Asp Tyr Pro Leu Ile Asp Asp Asp Val1 5 10 15Ile Pro Val Gly Asp Ser Ser Phe Ala Val Ser Ala Ser Thr Phe Ser 20 25 30Trp Pro Pro Pro Pro Ala Asn Val Ser Val Glu Ile Asp Ala Ser Leu 35 40 45Gly Asp Ser Asp Gly Leu Lys Asn Pro Ala Leu Lys Lys Arg Thr Lys 50 55 60Ser Asp Ser Ser Thr Ala Ser Ser Ser Lys Ala Cys Arg Glu Lys Leu65 70 75 80Arg Arg Asp Arg Leu Asn Asp Lys Phe Val Glu Leu Gly Ser Ile Leu 85 90 95Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ala Ser Ile Leu Ile Asp 100 105 110Ala Ala Arg Met Val Thr Gln Leu Arg Asp Glu Ala Leu Lys Leu Lys 115 120 125Asp Ser Asn Thr Ser Leu Gln Glu Lys Ile Lys Glu Leu Lys Ala Glu 130 135 140Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu145 150 155 160Lys Leu Glu Val Gln Val Lys Ser Met Asn Ala Gln Pro Ala Phe Leu 165 170 175Pro Pro Pro Pro Ala Ile Pro Ala Ala Phe Ala Pro Gln Gly Gln Ala 180 185 190Pro Gly Asn Lys Leu Val Pro Phe Ile Ser Tyr Pro Gly Val Ala Met 195 200 205Trp Gln Phe Met Pro Pro 21061233PRTSolanum tuberosum 61Met Val Ser Pro Glu Asn Thr Asn Trp Leu Tyr Asp Tyr Gly Phe Glu1 5 10 15Asp Ser Ser Val Pro Asp Ser Asn Phe Ser Ala Ser Ala Ser Gly Phe 20 25 30Asn Trp Pro Val Gln Asn Leu Asn Gly Ser Arg Asn Val Ser Ser Glu 35 40 45Ile Asp Gly Ser Ile Gly Glu Ser Asp Cys Pro Lys Glu Ser Gly Ser 50 55 60Lys Lys Arg Ala Arg Val Glu Ser Cys Ala Pro Thr Ser Ser Lys Ala65 70 75 80Cys Arg Glu Lys Leu Arg Arg Asp Lys Leu Asn Asp Lys Phe Met Glu 85 90 95Leu Gly Ala Leu Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ser 100 105 110Ala Ile Leu Val Asp Ala Val Arg Met Val Thr Gln Leu Arg Asp Glu 115 120 125Ala Gln Lys Leu Lys Asp Ser Asn Leu Asn Leu Gln Glu Lys Ile Lys 130 135 140Glu Leu Lys Val Glu Lys Thr Glu Leu Arg Asp Glu Lys Gln Arg Leu145 150 155 160Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Thr Thr Ser Ala 165 170 175Gln Pro Ser Phe Leu Pro Pro Ala Ile Pro Ser Ala Phe Ala Ala His 180 185 190Gly Gln Phe Pro Gly Ser Lys Leu Val Pro Ile Met Ser Tyr Pro Gly 195 200 205Val Ala Met Trp Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser Gln 210 215 220Asp His Val Leu Arg Pro Pro Val Ala225 23062192PRTCitrus reticulata 62Pro Ser Asn Gly Cys Val Glu Ile Asp Ser Ala Phe Gly Asp Ser Asn1 5 10 15Gly Leu Lys Glu Ser Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Gly 20 25 30Ser Ser Ser Ser Lys Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu 35 40 45Asn Asp Lys Phe Val Glu Leu Ala Ser Ile Leu Glu Pro Gly Arg Pro 50 55 60Pro Lys Thr Asp Lys Ala Ala Ile Leu Ile Asp Ala Val Arg Met Val65 70 75 80Thr Gln Leu Arg Ser Glu Ala Gln Lys Leu Lys Asp Ser Asn Ser Ser 85 90 95Leu Gln Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg 100 105 110Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu Lys Ile Glu Gln Gln 115 120 125Leu Lys Ala Met Ser Thr Gln Pro Ser Phe Leu Thr Pro Pro Ala Ile 130 135 140Pro Ala Ala Phe Ala Ala Gln Gly Gln Ala Pro Gly Asn Lys Leu Met145 150 155 160Pro Phe Ile Ser Tyr Pro Gly Val Ala Met Trp Gln Phe Met Pro Pro 165 170 175Ala Ala Val Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro Val Ala 180 185 19063230PRTThellungiella halophila 63Glu Asn Ala Asn Trp Ile Cys Asp Leu Ile Asp Ala Asp Tyr Gly Ser1 5 10 15Phe Thr Ile Gln Gly Pro Gly Phe Ser Trp Pro Val Gln Gln Pro Ile 20 25 30Gly Val Ser Ser Asn Ser Ser Ala Gly Val Asp Val Ser Ala Gly Asn 35 40 45Ser Glu Ala Ser Lys Glu Pro Gly Ser Lys Lys Arg Gly Arg Cys Glu 50 55 60Ser Ser Ser Ala Thr Gly Ser Lys Ala Cys Arg Glu Lys Leu Arg Arg65 70 75 80Asp Arg Leu Asn Asp Lys Phe Thr Glu Leu Gly Ala Ile Leu Glu Pro 85 90 95Gly Asn Pro Pro Lys Thr Asp Lys Ala Ala Ile Leu Val Asp Ala Val 100 105 110Arg Met Val Ala Gln Leu Arg Gly Glu Ala Gln Lys Leu Lys Asp Ser 115 120 125Asn Ser Ser Leu Gln Asp Lys Ile Lys Glu Leu Lys Thr Glu Lys Asn 130 135 140Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Thr Glu Lys Glu Lys Leu145 150 155 160Glu Gln Gln Leu Lys Thr Met Asn Ala Pro Gln Pro Ser Phe Phe Pro 165 170 175Ala Pro Pro Met Met Pro Thr Ala Phe Ala Ser Ala Gln Gly Gln Ala 180 185 190Pro Gly Asn Lys Met Val Pro Ile Ile Ser Tyr Pro Gly Val Ala Met 195 200 205Trp Gln Phe Met Pro Pro Ala Ser Val Asp Thr Ser Gln Asp His Val 210 215 220Leu Arg Pro Pro Val Ala225 23064238PRTPopulus tremuloides 64Glu Asn Asp Asn Trp Val Phe Asp Cys Gly Leu Ile Glu Asp Ile Ser1 5 10 15Val Pro Gly Gly Asp Leu Leu Gly Leu Glu Ser Leu Asp Glu Thr Pro 20 25 30Asn Gly Ser Leu Trp Ser Ser His Asn Phe Thr Asp Ser Ala Phe Leu 35 40 45Ser Val Glu Phe Asn Asn Ser Tyr Glu Asn Ser Asp Gly His Lys Glu 50 55 60Ser Gly Cys Arg Lys Arg Val Arg Pro Gly Ser Ser Asn Ala Thr Gly65 70 75 80Ser Lys Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Arg 85 90 95Phe Met Glu Leu Gly Ala Leu Leu Asp Pro Gly Arg Pro Pro Lys Val 100 105 110Asp Lys Ser Ala Ile Leu Val Asp Ala Ala Arg Met Val Thr Gln Leu 115 120 125Arg Asp Glu Ser Gln Lys Leu Lys Glu Ser Asn Val Ser Leu Gln Glu 130 135 140Lys Ile Asp Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys145 150 155 160Gln Arg Leu Lys Thr Glu Lys Glu Asn Leu Glu Arg Gln Val Lys Ala 165 170 175Leu Ser Ala Pro Pro Asn Phe Leu Pro His Pro Ser Ala Ile Pro Ala 180 185 190Pro Phe Ser Ala Pro Gly Gln Val Val Gly Ser Lys Met Met Pro Phe 195 200 205Val Gly Tyr Pro Gly Ile Ser Met Trp Gln Phe Met Pro Pro Ala Val 210 215 220Val Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro Val Ala225 230 23565232PRTVitis vinifera 65Met Val Ser Pro Glu Glu Asp Pro Asn Trp Ile Phe Asp Tyr Gly Leu1 5 10 15Ile Asp Asp Val Pro Val Pro Ser Leu Gln Ala Thr Phe Asn Trp Pro 20 25 30Ser His Asp Phe Thr Ala Ser Val Ala Leu Gly Val Glu Phe Asp Asp 35 40 45Ser Pro Val Asn Leu Asp Asp Val Lys Glu Asn His Ser Arg Lys Arg 50 55 60Met Arg Ser Gly Leu Cys Ser Ala Ser Gly Ser Lys Ala Cys Arg Glu65 70 75 80Lys Val Arg Arg Asp Arg Leu Asn Asp Arg Phe Leu Glu Leu Gly Ser 85 90 95Ile Leu Glu Pro Gly Arg Pro Pro Lys Met Asp Lys Ala Val Ile Leu 100 105 110Ser Asp Ala Leu Arg Met Met Thr Gln Leu Arg Ser Glu Gly Gln Lys 115 120 125Leu Lys Lys Ser Cys Glu Asp Leu Gln Glu Lys Ile Asn Glu Leu Lys 130 135 140Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Thr Glu145 150 155 160Lys Glu Asn Ile Val Gln Gln Ile Lys Ala Leu Ser Thr Gln Ala Gly 165 170 175Phe Leu Pro His Pro Ser Ala Ile Pro Ala Pro Phe Ala Ala Pro Gly 180 185 190Gln Val Val Gly Ser Lys Leu Met Pro Phe Ile Gly Tyr Pro Gly Val 195 200 205Ser Met Trp Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser Gln Asp 210 215 220His Val Leu Arg Pro Pro Val Ala225 23066191PRTBrassica napusmisc_feature(2)..(2)Xaa can be any naturally occurring amino acid 66Ser Xaa Val Met Leu Ser Trp Lys Phe Arg Ser His Gln Gly Thr Cys1 5 10 15Ser Lys Lys Arg Ala Arg Cys Glu Ser Ser Ser Ala Thr Ser Ser Lys 20 25 30Ala Cys Arg Glu Lys Gln Arg Arg Asp Arg Leu Asn Asp Lys Phe Met 35 40 45Glu Leu Gly Ala Ile Leu Glu Pro Gly Asn Pro Pro Xaa Thr Asp Lys 50 55 60Ala Ala Ile Leu Val Asp Ala Val Arg Met Val Thr Gln Leu Arg Gly65 70 75 80Glu Ala Gln Lys Leu Lys Asp Ser Asn Ser Ser Leu Gln Asp Lys Ile 85 90 95Lys Glu Leu Lys Thr Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg 100 105 110Leu Lys Thr Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Ala Met Asn 115 120 125Ala Pro Pro Gln Pro Ser Phe Phe Pro Ala Pro Pro Met Met Pro Thr 130 135 140Ala Phe Ala Ser Ala Gln Gly Gln Ala Pro Gly Asn Lys Met Val Pro145 150 155 160Val Ile Ser Tyr Pro Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala 165 170 175Ser Val Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro Val Ala 180 185 19067181PRTVitis vinifera 67Asp Ser Asp Gly Ile Lys Glu Thr Gly Ser Lys Lys Arg Val Arg Ser1 5 10 15Glu Ser Cys Gly Ala Ser Ser Ser Lys Ala Cys Arg Glu Lys Leu Arg 20 25 30Arg Asp Arg Leu Asn Asp Lys Ser Met Glu Leu Gly Ser Ile Leu Glu 35 40 45Pro Gly Arg Pro Pro Lys Thr Asp Lys Ser Ser Ile Leu Ile Asp Ala 50 55 60Val Arg Met Val Thr Gln Leu Arg Gly Glu Ser Gln Lys Leu Lys Asp65 70 75 80Ser Asn Ser Ser Leu Gln Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys 85 90 95Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu Lys 100 105 110Leu Glu Gln Gln Leu Lys Ala Met Asn Ala Gln Pro Ser Phe Leu Pro 115 120 125Pro Val Pro Ser Ile Pro Ala Ala Phe Ala Ala Gln Gly Gln Ala Gly 130 135 140Gly Asn Lys Leu Val Pro Phe Ile Gly Tyr Pro Gly Val Ala Met Trp145 150 155 160Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser Gln Asp His Val Leu 165 170 175Arg Pro Pro Val Ala 18068237PRTGossypium raimondii 68Met Val Ser Pro Glu Asn Thr Asn Tyr Trp Ser Ser Phe Asp Tyr Ala1 5 10 15Thr Leu Ile Asn Asp Ile Pro Ala Pro Asp Gly Pro Tyr Ser Gly Phe 20 25 30Ser Trp Pro Thr Arg Pro Ile Asn Ala Ser Ser Asn Val Phe Ser Val 35 40 45Glu Thr Asp Gly Ser Phe Glu Asp Ser Asp Gly Leu Lys Glu Ser Gly 50 55 60Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Asn Val Ser Ser Ser Lys65 70 75 80Ala Cys Arg Glu Lys Leu Arg Arg Asp Lys Leu Asn Glu Lys Phe Met 85 90 95Glu Leu Ser Ser Ile Leu Glu Pro Glu Lys Pro Pro Lys Thr Asp Lys 100 105 110Ala Ala Ile Leu Val Asp Ala Val Arg Met Val Thr Gln Leu Arg Gly 115 120 125Glu Ala Gln Lys Leu Lys Asp Ser Ile Ser Ser Leu His Asp Arg Ile 130 135 140Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg145 150 155 160Leu Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Ala Met Asn 165 170 175Ser Gln Pro Ser Phe Met Pro Pro Ala Pro Ala Phe Pro Ala Ala Phe 180 185 190Ala Thr Ala Gln Gly Gln Val Pro Gly Asn Lys Leu Val Pro Phe Phe 195 200 205Gly Tyr Pro Gly Val Ala Met Trp Gln Phe Met Leu Pro Ala Ser Leu 210 215 220Asp Thr Ser Glu Asp His Val Leu Arg Pro Pro Val Ala225 230 23569238PRTPopulus tremuloides 69Glu Asn Asp Asn Trp Val Phe Asp Cys Gly Leu Ile Glu Asp Ile Ser1 5 10 15Val Pro Gly Gly Asp Leu Leu Gly Leu Glu Ser Leu Asp Glu Thr Pro 20 25 30Asn Gly Ser Leu Trp Ser Ser His Asn Phe Thr Asp Ser Ala Phe Leu 35 40 45Ser Val Glu Phe Asn Asn Ser Tyr Glu Asn Ser Asp Gly His Lys Glu 50 55 60Ser Gly Cys Arg Lys Arg Val Arg Pro Gly Ser Ser Asn Ala Thr Gly65 70 75 80Ser Lys Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Arg 85 90 95Phe Met Glu Leu Gly Ala Leu Leu Asp Pro Gly Arg Pro Pro Lys Val 100 105 110Asp Lys Ser Ala Ile Leu Val Asp Ala Ala Arg Met Val Thr Gln Leu 115 120 125Arg Asp Glu Ser Gln Lys Leu Lys Glu Ser Asn Val Ser Leu Gln Glu 130 135 140Lys Ile Asp Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys145 150 155 160Gln Arg Leu Lys Thr Glu Lys Glu Asn Leu Glu Arg Gln Val Lys Ala 165 170 175Leu Ser Ala Pro Pro Asn Phe Leu Pro His Pro Ser Ala Ile Pro Ala 180 185 190Pro Phe Ser Ala Pro Gly Gln Val Val Gly Ser Lys Met Met Pro Phe 195 200 205Val Gly Tyr Pro Gly Ile Ser Met Trp Gln Phe Met Pro Pro Ala Val 210 215 220Val Asp Thr Ser Gln Asp His Val Leu Arg Pro Ser Val Ala225 230 23570170PRTPrunus dulcis 70Lys Arg Val Arg Thr Glu Ser Cys Ser Gly Ser Ser Ser Lys Ala Cys1

5 10 15Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe Leu Glu Leu 20 25 30Gly Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ala Ala 35 40 45Ile Leu Val Asp Ala Val Arg Met Val Asn Gln Leu Arg Gly Glu Ala 50 55 60Gln Lys Leu Lys Asp Ser Asn Ser Ser Leu Gln Glu Lys Ile Lys Glu65 70 75 80Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys 85 90 95Leu Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Ala Met Asn Ala Gln 100 105 110Pro Gly Phe Leu Pro Pro Pro Pro Ala Ile Pro Ala Ala Phe Ala Ala 115 120 125Gln Gly Gln Ala His Gly Asn Lys Leu Val Pro Phe Ile Gly Tyr Pro 130 135 140Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala Ser Val Asp Thr Ser145 150 155 160Gln Asp His Val Leu Arg Pro Pro Val Ala 165 17071170PRTGlycine max 71Lys Arg Thr Lys Ser Asp Ser Ser Thr Ala Ser Ser Ser Lys Ala Cys1 5 10 15Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe Val Glu Leu 20 25 30Gly Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ala Ser 35 40 45Ile Leu Ile Asp Ala Ala Arg Met Val Thr Gln Leu Arg Asp Glu Ala 50 55 60Leu Lys Leu Lys Asp Ser Asn Thr Ser Leu Gln Glu Lys Ile Lys Glu65 70 75 80Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys 85 90 95Ala Glu Lys Glu Lys Leu Glu Val Gln Val Lys Ser Met Asn Ala Gln 100 105 110Pro Ala Phe Leu Pro Pro Pro Pro Ala Ile Pro Ala Ala Phe Ala Pro 115 120 125Gln Gly Gln Ala Pro Gly Asn Lys Leu Val Pro Phe Ile Ser Tyr Pro 130 135 140Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser145 150 155 160Gln Asp His Val Leu Arg Pro Pro Val Ala 165 17072181PRTMedicago truncatula 72Asp Ser Asp Gly Leu Lys Glu Ser Gly Ser Lys Lys Arg Val Arg Ser1 5 10 15Glu Ser Cys Ala Ala Thr Ser Ser Lys Ala Cys Arg Glu Lys Leu Arg 20 25 30Arg Asp Arg Leu Asn Asp Lys Phe Ile Glu Leu Gly Ser Ile Leu Glu 35 40 45Pro Gly Arg Pro Ala Lys Thr Asp Lys Ala Ala Ile Leu Ile Asp Ala 50 55 60Val Arg Met Val Thr Gln Leu Arg Gly Glu Ala Gln Lys Leu Lys Asp65 70 75 80Ala Asn Ser Gly Leu Gln Glu Lys Ile Lys Glu Leu Lys Val Glu Lys 85 90 95Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu Lys 100 105 110Leu Glu Gln Gln Leu Lys Ser Met Asn Ala Pro Pro Ser Phe Leu Pro 115 120 125Thr Pro Thr Ala Leu Pro Ala Ala Phe Ala Ala Gln Gly Gln Ala His 130 135 140Gly Asn Lys Leu Val Pro Phe Ile Ser Tyr Pro Gly Val Ala Met Trp145 150 155 160Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser Gln Asp His Val Leu 165 170 175Arg Pro Pro Val Ala 18073189PRTMedicago truncatula 73Ala Glu Val Asp Gly Ser Leu Gly Asp Ser Asp Gly Leu Lys Glu Ser1 5 10 15Gly Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Ala Ala Thr Ser Ser 20 25 30Lys Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe 35 40 45Ile Glu Leu Gly Ser Ile Leu Glu Pro Gly Gly Pro Ala Lys Thr Asp 50 55 60Lys Ala Ala Ile Leu Ile Asp Ala Val Arg Met Val Thr Gln Leu Arg65 70 75 80Gly Glu Ala Gln Lys Leu Lys Asp Ala Asn Ser Gly Leu Gln Glu Lys 85 90 95Ile Lys Glu Leu Lys Val Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln 100 105 110Arg Leu Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Ser Met 115 120 125Asn Ala Pro Pro Ser Phe Leu Pro Thr Pro Thr Ala Leu Pro Ala Ala 130 135 140Phe Ala Ala Gln Gly Gln Ala His Gly Asn Lys Leu Val Pro Phe Ile145 150 155 160Ser Tyr Pro Gly Val Ala Met Trp Gln Phe Met Pro Pro Ala Ala Val 165 170 175Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro Val Ala 180 18574169PRTGlycine max 74Arg Ala Arg Cys Asp Ser Ser Thr Ala Ser Ser Ser Lys Ala Cys Arg1 5 10 15Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe Val Glu Leu Gly 20 25 30Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ala Ala Ile 35 40 45Leu Ile Asp Ala Ala Arg Met Val Thr Gln Leu Arg Asp Glu Ala Leu 50 55 60Lys Leu Lys Asp Ser Asn Thr Ser Leu Gln Glu Lys Ile Lys Glu Leu65 70 75 80Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala 85 90 95Glu Lys Glu Lys Leu Glu Met Gln Val Lys Ser Met Asn Ala Gln Pro 100 105 110Ala Phe Leu Pro Pro Pro Pro Ala Ile Pro Ala Ala Phe Ala Pro Gln 115 120 125Gly Gln Ala Pro Gly Asn Lys Leu Met Pro Phe Ile Arg Tyr Pro Gly 130 135 140Val Ala Met Trp Gln Phe Met Pro Pro Ala Thr Met Asp Thr Ser Gln145 150 155 160Asp His Val Leu Arg Pro Pro Val Ala 16575158PRTVitis vinifera 75Thr Lys Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Glu Arg1 5 10 15Phe Leu Glu Leu Gly Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr 20 25 30Asp Lys Ala Ala Ile Leu Ser Asp Ala Val Arg Met Val Thr Gln Leu 35 40 45Arg Ser Glu Ala Gln Lys Leu Lys Glu Ser Asn Gly Asp Leu Gln Glu 50 55 60Lys Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys65 70 75 80Gln Arg Leu Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Val Lys Ala 85 90 95Ile Ser Ala Gln Pro Gly Phe Leu Pro His Pro Ser Ala Met Pro Ala 100 105 110Ala Phe Ala Ala Gln Gly Arg Ala Pro Gly Asn Lys Leu Met Pro Phe 115 120 125Ile Gly Tyr Pro Ser Val Ala Met Trp Gln Phe Met Pro Pro Ala Ala 130 135 140Val Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro Val Ala145 150 15576181PRTGlycine soja 76Asp Ser Asp Ser Leu Lys Glu Ser Gly Ser Lys Lys Arg Val Arg Ser1 5 10 15Glu Ser Cys Ala Ala Ser Gly Ser Lys Ala Cys Arg Glu Lys Leu Arg 20 25 30Arg Asp Arg Leu Asn Asp Lys Phe Val Glu Leu Gly Ala Ile Leu Glu 35 40 45Pro Gly Arg Pro Ala Lys Thr Asp Lys Ala Ala Ile Leu Ile Asp Ala 50 55 60Val Arg Met Val Thr Gln Leu Arg Gly Glu Ala Gln Lys Leu Lys Asp65 70 75 80Thr Asn Gln Gly Leu Gln Glu Lys Ile Lys Glu Leu Lys Ala Glu Lys 85 90 95Asn Glu Leu Arg Asp Glu Lys Gln Arg Leu Lys Ala Glu Lys Glu Lys 100 105 110Leu Glu Gln Gln Leu Lys Ser Leu Asn Ala Gln Pro Ser Phe Met Pro 115 120 125Pro Pro Ala Ala Met Pro Ala Ala Phe Ala Ala Gln Gly Gln Ala His 130 135 140Gly Asn Lys Leu Val Pro Phe Ile Ser Tyr Ser Gly Ser Cys Met Trp145 150 155 160Gln Phe Met Pro Pro Ala Ala Val Asp Thr Ser Gln Asp His Val Leu 165 170 175Arg Pro Pro Val Ser 18077235PRTSolanum tuberosum 77Asn Trp Leu Phe Asp Tyr Glu Leu Ile Thr Asp Ile Thr Ser Ala Ala1 5 10 15Ser Val Thr Val Thr Asp Phe Gln Ser Pro Ala Thr Ile Asp Phe Ser 20 25 30Trp Pro Ala Gln Thr Ile Tyr Ala Ser Ser Asn Leu Ile Ala Glu Thr 35 40 45Asp Tyr Thr Phe Ala Asp Ser Glu Val Ser Lys Glu Ala Ser Ser Arg 50 55 60Lys Arg Leu Lys Ser Glu Trp Cys Ser Ser Pro Arg Ser Lys Ala Cys65 70 75 80Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Glu Arg Phe Leu Glu Leu 85 90 95Ser Ser Val Leu Asp Pro Gly Arg Pro Pro Lys Thr Glu Lys Val Ala 100 105 110Ile Leu Ser Asp Ala Gln Arg Met Leu Ile Glu Leu Arg Thr Glu Thr 115 120 125Gln Lys Leu Lys Glu Ser Asn Glu Glu Leu Gln Glu Lys Ile Lys Glu 130 135 140Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Arg Arg Leu Lys145 150 155 160Glu Glu Lys Glu Asn Leu Glu Gln Gln Val Lys Ser Leu Ala Ser Lys 165 170 175Pro Gly Phe Leu Ser His Pro Ser Ala Val Gly Ala Ala Phe Thr Ala 180 185 190Gln Gly Gln Val Ala Ala Gly Asn Lys Leu Met Pro Phe Ile Gly Tyr 195 200 205Pro Ser Val Ala Met Trp Gln Phe Met Gln Pro Ala Val Val Asp Thr 210 215 220Ser Gln Asp His Val Leu Arg Pro Pro Val Ala225 230 23578229PRTGossypium raimondii 78Met Val Ser Pro Glu Asn Thr Asn Tyr Trp Ser Ser Phe Asp Tyr Ala1 5 10 15Thr Leu Ile Asn Asp Ile Pro Ala Pro Asp Gly Pro Tyr Ser Gly Phe 20 25 30Ser Trp Pro Thr Arg Pro Ile Asn Ala Ser Ser Asn Val Phe Ser Val 35 40 45Glu Ile Asp Gly Ser Phe Glu Asp Ser Asp Gly Leu Lys Glu Ser Gly 50 55 60Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Asn Val Ser Ser Ser Lys65 70 75 80Ala Cys Arg Glu Lys Leu Arg Arg Asp Lys Leu Asn Glu Lys Phe Met 85 90 95Glu Leu Ser Ser Ile Leu Glu Pro Glu Lys Pro Pro Lys Thr Asp Lys 100 105 110Ala Ala Ile Leu Val Asp Ala Val Arg Met Val Thr Gln Leu Arg Gly 115 120 125Glu Ala Gln Lys Leu Lys Asp Ser Ile Ser Ser Leu His Asp Arg Ile 130 135 140Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln Arg145 150 155 160Leu Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Ala Met Asn 165 170 175Ser Gln Pro Ser Phe Met Pro Pro Ala Pro Ala Phe Pro Ala Ala Phe 180 185 190Ala Thr Ala Gln Gly Gln Val Pro Gly Asn Lys Leu Val Pro Phe Phe 195 200 205Gly Tyr Pro Gly Val Ala Met Trp Gln Phe Met Leu Pro Ala Ser Leu 210 215 220Asp Thr Ser Glu Asp22579218PRTVitis viniferamisc_feature(197)..(197)Xaa can be any naturally occurring amino acid 79Met Val Ser Pro Glu Ala Thr Asn Trp Leu Tyr Glu Tyr Gly Leu Ile1 5 10 15Glu Asp Ile Pro Val Pro Asp Ser Asn Phe Ala Asn Thr Asn Ser Gly 20 25 30Phe Ala Trp Thr Pro Val Gln Ala Leu Asn Thr Ser Ala Asn Val Ser 35 40 45Gly Glu Ile Asp Gly Ser Phe Gly Asp Ser Asp Gly Ile Lys Glu Thr 50 55 60Gly Ser Lys Lys Arg Val Arg Ser Glu Ser Cys Gly Ala Ser Ser Ser65 70 75 80Lys Ala Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Ser 85 90 95Met Glu Leu Gly Ser Ile Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp 100 105 110Lys Ser Ser Ile Leu Ile Asp Ala Val Arg Met Val Thr Gln Leu Arg 115 120 125Gly Glu Ser Gln Lys Leu Lys Asp Ser Asn Ser Ser Leu Gln Glu Lys 130 135 140Ile Lys Glu Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Gln145 150 155 160Arg Leu Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Ala Met 165 170 175Asn Ala Gln Pro Ser Phe Leu Pro Pro Val Pro Ser Ile Pro Ala Ala 180 185 190Phe Ala Ala Gln Xaa Gln Ala Gly Gly Asn Lys Leu Val Pro Phe Ile 195 200 205Gly Tyr Pro Gly Val Ala Met Trp Gln Phe 210 21580222PRTLycopersicon esculentum 80Met Val Ser Pro Glu Ser Thr Asn Trp Leu Tyr Asp Tyr Gly Phe Glu1 5 10 15Asp Ser Cys Val Pro Asp Ser Asn Phe Ser Ala Ser Ala Ser Gly Phe 20 25 30Asn Trp Ser Val Gln Asn Leu Asn Gly Ser Arg Asn Val Ser Ser Glu 35 40 45Ile Asp Gly Ser Ile Gly Glu Ser Asp Tyr Pro Lys Glu Ser Gly Ser 50 55 60Lys Lys Arg Ala Arg Val Glu Ser Cys Ala Pro Thr Ser Ser Lys Ala65 70 75 80Cys Arg Glu Lys Leu Arg Arg Asp Arg Leu Asn Asp Lys Phe Met Glu 85 90 95Leu Gly Ala Leu Leu Glu Pro Gly Arg Pro Pro Lys Thr Asp Lys Ser 100 105 110Ala Ile Leu Val Asp Ala Val Arg Leu Val Thr Gln Leu Arg Asp Glu 115 120 125Ala Gln Lys Leu Lys Asp Ser Asn Leu Asn Leu Gln Glu Lys Ile Lys 130 135 140Glu Leu Lys Val Glu Lys Thr Glu Leu Arg Asp Glu Lys His Arg Leu145 150 155 160Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Leu Lys Thr Thr Ser Ala 165 170 175Arg Pro Ser Tyr Leu Pro Pro Ala Ile Pro Ser Ala Phe Ala Ala His 180 185 190Gly Gln Phe Pro Gly Ser Lys Leu Val Pro Ile Met Ser Tyr Pro Cys 195 200 205Val Pro Met Trp Gln Phe Met Pro Pro Ala Ala Val Asp Thr 210 215 22081238PRTPinus taeda 81Met Ser Ser Pro Gln Ser Asn Lys Trp Leu Ser Tyr Phe Asp Glu Pro1 5 10 15Leu Leu Asp Asp Val Gly Val Gly Gln Pro Ala Asn Pro Phe Phe Trp 20 25 30Cys Gly Gln Gly Ile Asn Asp Gln Pro Asp Val Ser Gly Ser Val Glu 35 40 45Ile Asp Gly Pro Asn Lys Asp Met Asp Glu Gln Asp Lys Leu Cys Pro 50 55 60Arg Lys Arg Ser Arg Glu Glu Ser Ser Gly Gly Pro Gly Ser Lys Ala65 70 75 80Cys Arg Glu Lys Met Arg Arg Asp Arg Leu Asn Asp Arg Phe Met Glu 85 90 95Leu Ser Ser Val Leu Glu Pro Gly Arg Pro Pro Lys Thr Ala Asp Lys 100 105 110Ala Thr Ile Leu Ser Asp Ala Ala Arg Val Met Thr Gln Leu Arg Thr 115 120 125Glu Ala Gln Asn Leu Lys Ala Glu Asn Glu Arg Leu Gln Glu Ala Ile 130 135 140Lys Asp Leu Lys Ala Glu Lys Asn Glu Leu Arg Asp Glu Lys Leu Arg145 150 155 160Met Lys Ala Glu Lys Glu Lys Leu Glu Gln Gln Val Lys Ala Met Ala 165 170 175Leu Pro Thr Gly Phe Val Pro His Pro Ala Ala Phe His Ala Ala Ala 180 185 190Ala Phe Ala Ala Gln Ser Gln Ala Ala Ala Asn Lys Thr Met Pro Val 195 200 205Pro Gly Tyr Pro Gly Met Ala Met Trp Gln Trp Met Pro Pro Ala Val 210 215 220Val Asp Thr Ser Gln Asp His Val Leu Arg Pro Pro Val Ala225 230 23582783DNALolium perenne 82tccgtcgcca tggcgtcccc ggagggcgcc aactgggtct tcgactgccc gctcatggac 60gaccttgctg ccgccgactt caccgcaccg cccgcaggag gcttctactg ggcaccaccg 120atgcagccgc agatgcacac ccaggccccg gccgtctccg ccaccccgcc tcccaaccac 180tgtgccgaaa tcaatagccc tatttctgtg gactgggacc atgccaaagg acagccaaca 240aataaacgtc ctaggtcaga atctggtgct caacccagct ccaaagcatg cagggagaaa 300gcgagaaggg acaagctaaa cgagaggttc ttggaattgg gtgctgtctt ggatccaggg 360aaaacaccta aaatcgacaa gtgtgctata ttgaatgatg ctattcgtgc ggtgactgag 420ctacgtagtg aagcagagaa gctgaaggat tcaaacgagt ctctccaaga gaagatcaaa 480gagctgaagg ctgagaagaa tgagctgcgg

gatgagaagc aaaagctgaa ggcagagaaa 540gagagcctgg agcagcagat caagttcatg aatgcccgtc agagcctcgt accacaccta 600ccgcaccctt cggttatccc agcggctgca tttgctgctc cccaagggca agtgccaggg 660cagaagctga tgatgcctgt cattggctac catggatttc ccatgtggca attcatgcca 720ccttctgatg ttgatacctc cgatgatccc aagtcgtgcc ctcctgttgc ataagccagc 780taa 783831040DNAOryza sativa 83gtgagcggcc ccaccccccc gcagccatgg cctccccgga gggctccacg tgggtcttcg 60actgccctct gatggacgac ctcgccgccg ccgccggctt cgacgccgcc cccgccggag 120gcttctactg gacgacgccc gctcctccgc aggcggcgct acagccgccg ccgccgcagc 180agcagcccgt cgcccctgcc accgcggctc cgaacgcctg tgctgaaatc aatggctctg 240tggactgtga acatggcaaa gaacagccaa caaataaacg tccgagatca gaaagtggca 300ctcgaccaag ctccaaagca tgcagggaaa aagtaagaag ggacaagttg aacgagaggt 360tcttggaact gggtgctgtc ctggaaccag ggaagacacc caaaatggac aaatcgtcta 420tattgaacga tgctattcgt gtaatggctg agctgcgtag tgaggcacag aagttgaagg 480aatcaaatga gagtctccaa gagaaaatca aagagttgaa ggctgagaaa aacgagctgc 540gtgatgagaa gcaaaagctg aaggcagaga aagagagcct ggagcagcag ataaagttcc 600tgaatgctcg accaagcttc gtaccacacc ctccggttat cccagccagt gcattcactg 660ctcctcaagg gcaagctgcc gggcagaagc tgatgatgcc tgtgattggc tacccaggat 720ttccgatgtg gcagttcatg ccgccttctg atgttgatac cacagatgac accaagtcat 780gccctcctgt tgcataagtc aaagcaaaga tcaatttgcc tcgccttgta ggaaagaggt 840gaaactgcct tccattcaag cccagtttgg tcgtcagtgt ttaaactacc tagctaatcc 900caggattaaa ccgaagcttc gctgtatcga agtatcaacc ggtgacatgt gaactgacga 960aagatgacac cgttgtatat tacatattag taaataaatt ccatctgtcc aattaaatga 1020gaattagatg ccatataatt 104084762DNAOryza sativa 84atgtccggta ccccggcgga cggcggcggc ggaggcggcg gaggcggcgg agggagcggg 60gacgactggt tcctcgactg cggcatcctc gaggacctcc cggcggccgc gtgcggggcc 120ttcccgtggg acgcatcccc gtcctgctcc aacccgagtg tggaagtaag cagctatgtg 180aacaccacct cttatgtcct caaggaacct ggcagtaata aacgtgtaag gtcagggtct 240tgtggtaggc caacatcaaa agcttccagg gaaaagatca gaagagataa gatgaatgat 300aggtttcttg aattggggac tactttggag cctgggaagc cagtaaaatc tgacaaagct 360gctatcctaa gtgatgccac tcgcatggta attcaacttc gtgctgaagc gaagcagcta 420aaggatacta acgagagtct tgaagataag attaaagaac tgaaggcaga gaaggacgag 480cttcgtgatg agaagcagaa gctgaaagta gagaaggaga cattagagca gcaggtgaag 540attctgactg caactccagc ctacatgcct caccccacat tgatgccagc accgtaccca 600caagcaccac ttgccccctt ccaccatgcc caggggcaag ccgcggggca gaagctgatg 660atgccttttg ttggataccc ggggtacccg atgtggcagt tcatgccgcc ttccgaagtc 720gacacctcca aggacagcga agcgtgcccg cctgtcgcgt aa 76285711DNAOryza sativa 85atggacggag gcggagaccc cgttgacgag ttcctcatcg gcggcggcgg cgaggacggc 60gacctcggcg tcttctgcga cggtgtgccg acgctgccct gcgatggagg acttgggatt 120gatgatgtca gtggagatac ttgttgcctt gaccaatctg ttttagggaa aagaggtaga 180gatgaatcat catcatctgg tccaaagtcc aaagcttgcc gtgagaaaat tcggagggat 240agactgaatg acaggttcct tgagttatct tccgttatca atcctgacaa gcaagctaag 300ttggataaag caaatatctt gagtgatgca gctcgtctgt tggcagaact cagaggcgag 360gcagaaaagc ttaaagaatc taatgagaag ctacgagaaa caatcaagga ccttaaggtg 420gagaagaatg aactccgtga tgagaaagtt actctgaagg cagagaagga gaggctggaa 480cagcaagtta aagcattgag tgttgctcct acaggatttg ttcctcatct ccctcatcca 540gctgcattcc atcccgctgc attccctcca tttataccac cttatcaagc tctgggcaac 600aaaaatgctc ctacgcccgc agcattccaa gggatggcaa tgtggcagtg gttgcctcca 660actgctgtgg acacaactca agatccaaag ctttggccac caaatgctta a 71186775DNATriticum aestivum 86ctgccccctc atggacgacc ttgctgccgc cgacttcgcc gcggcatccg caggaggctt 60ctactggacc ccgccgatgc agccgcagat gcacactctt gcgcaggccg tctccgccac 120cccggctccc aatccctgtg ctgaaatcaa tagctctgtt tcggtggact gggaccatgc 180caaaggacaa ccgaaaaata aacgtcctag gtcagaaact ggtgctcaac ctagctccaa 240agcatgcagg gagaaagtga gaagggacaa gctaaacgag aggttcttgg aattgggtgc 300tgtcttggat ccggggaaaa cacctaaaat cgacaaatgt gctatattaa atgatgctat 360ccgtgcggta actgaattgc gtagtgaagc acagaagttg aaggattcca atgagtctct 420ccaagagaag attagagagc taaaggctga caagaatgag ctacgacatg agaagcaaaa 480gatgaaggcg gagaaagaga gcctggagca gcagattaag ttcatgaatg cccgtcagag 540cctcgtacca cacccttctg tcatcccagc tgctgcattc gctgccgccc aaggccaagc 600ggcagggcac aagctgatga tgcctgtgat gagctaccca ggatttccca tgtggcagtt 660catgccgcct tcagatgttg atacctccga tgaccccaag tcatgccctc cggttgcata 720agccagcaaa aatcatttgc ctcatctatc tcatggggaa ggatggctaa aaagc 77587674DNAHordeum vulgare 87ccgcgaaacc ctagtccccc ggcaaccttc gctggacccg gggagccgct ccggcgccat 60ggcatccccg gaaggatcaa actgggtctt cgactgcccc ctcatggacg accttgctgc 120cgccgacttc gccgcggtac ccgcaggagg cttctactgg aacccgccga tgccgccgca 180gatgcacact ctggcgcagg ccgtctccgc caccccggct cccaatccct gtgctgaaat 240caatagctct gtttcggtgg actgggacca tgccaaagga caaccgaaaa ataaacgtcc 300tagatcagaa actggtgctc aacctagctc caaagcatgc agggagaaag ttagaaggga 360caagctaaat gagaggttct tggaattggg tgctgtcttg gacccgggga aaacacctaa 420aatcgacaaa tgtgctatat taaatgatgc tatccgtgcg gtaactgaat tgcgtagtga 480agcagagaag ttgaaggatt caaatgagtc tctccaagag aagattagag agctgaaggc 540tgagaagaat gagctgcgag atgagaagca aaagctgaag gcggaaaaag agagcctgga 600gcagcagatt aagttcatga atgcccgtca gagactcgta ccacaccctt ctgtcatccc 660agctactgca ttcg 67488820DNAOryza sativa 88ccgcccccaa atctcctcgc gacctcgaaa ccctagcctc ctccggccac cgtcgccggc 60cacggtgagc ggccccaccc ccccgcagcc atggcctccc cggagggctc cacgtgggtc 120ttcgactgcc ctctgatgga cgacctcgcc gccgccgccg gcttcgacgc cgcccccgcc 180ggaggcttct actggacgac gcccgctcct ccgcaggcgg cgctacagcc gccgccgccg 240cagcagcagc ccgtcgcccc tgccaccgcg gctccgaacg cctgtgctga aatcaatggc 300tctgtggact gtgaacatgg caaagaacag ccaacaaata aacgtccgag atcagaaagt 360ggcactcgac caagctccaa agcatgcagg gaaaaagtaa gaagggacaa gttgaacgag 420aggttcttgg aactgggtgc tgtcctggaa ccagggaaga cacccaaaat ggacaaatcg 480tctatattga acgatgctat tcgtgtaatg gctgagctgc gtagtgaggc acagaagttg 540aaggaatcaa atgagagtct ccaagagaaa atcaaagagt tgaaggctga gaaaaacgag 600ctgcgtgatg agaagcaaaa gctgaaggca gagaaagaga gcctggagca gcagataaag 660ttcctgaatg ctcgaccaag cttcgtacca caccctccgg ttatcccagc cagtgcattc 720actgctcctc aagggcaagc tgccgggcag aagctgatga tgcctgtgat tggctaccca 780ggatttccga tgtggcagtt catgccgcct tctgatgttg 82089703DNATriticum aestivummisc_feature(34)..(34)n is a, c, g, or t 89aggaattcgg cacgaggccg cttccccgct accnccccaa ccccgaaata tcccccaatt 60ccgacgcgac cgcggaaccc tagccccccg gcaatcttcg ctggacccgg agagccgctc 120cggcgccatg gcatccccgg aaggatcaaa ctgggtattc gactgccccc tcatggacga 180ccttgctgcc gccgacttcg ccgcggcatc cgcaggaggc ttctactgga ccccgccgat 240gcagccgcag atgcacactc ttgcgcaggc cgtctccgcc accccggctc ccaatccctg 300tgctgaaatc aatagctctg tttcggtgga ctgggaccat gccaaaggac aaccgaaaaa 360taaacgtcct aggtcagaaa ctggtgctca acctagctcc aaagcatgca gggagaaagt 420gagaagggac aagctaaacg agaggttctt ggaattgggt gctgtcttgg atccggggaa 480aacacctaaa atcgacaaat gtgctatatt aaatgatgct atccgtgcgg taactgaatt 540gcgtagtgaa gcagagaagt tgaaggattc caatgagtct ctccaagaga agattagaga 600gctaaaggct gagaagaatg agctacgaga tgagaagcaa aagttgaagg cggagaaaga 660gagcctggag cagcagatta agttcatgaa tgcccgtcag agc 70390586PRTTriticum aestivum 90Cys Cys Gly Cys Thr Cys Cys Gly Gly Cys Gly Cys Cys Ala Thr Gly1 5 10 15Gly Cys Ala Thr Cys Cys Cys Cys Gly Gly Ala Ala Gly Gly Ala Thr 20 25 30Cys Ala Ala Ala Cys Thr Gly Gly Gly Thr Ala Thr Thr Cys Gly Ala 35 40 45Cys Thr Gly Cys Cys Cys Thr Cys Thr Cys Ala Thr Gly Gly Ala Cys 50 55 60Gly Ala Cys Cys Thr Thr Gly Cys Thr Gly Cys Cys Gly Cys Cys Gly65 70 75 80Ala Cys Thr Thr Cys Gly Cys Cys Gly Cys Gly Gly Cys Ala Thr Cys 85 90 95Cys Ala Cys Ala Gly Gly Ala Gly Gly Cys Thr Thr Cys Thr Ala Cys 100 105 110Thr Gly Gly Ala Cys Cys Cys Cys Gly Cys Cys Gly Ala Thr Gly Cys 115 120 125Ala Gly Cys Cys Gly Cys Ala Gly Ala Thr Gly Cys Ala Cys Ala Cys 130 135 140Thr Cys Thr Thr Gly Cys Gly Cys Ala Gly Gly Cys Cys Gly Thr Cys145 150 155 160Thr Cys Cys Gly Cys Cys Ala Cys Cys Cys Cys Gly Gly Cys Thr Cys 165 170 175Cys Cys Ala Ala Thr Cys Cys Cys Thr Gly Thr Gly Cys Thr Gly Ala 180 185 190Ala Ala Thr Cys Ala Ala Thr Ala Gly Cys Thr Cys Thr Gly Thr Thr 195 200 205Thr Cys Gly Gly Thr Gly Gly Ala Cys Thr Gly Gly Gly Ala Cys Cys 210 215 220Ala Thr Gly Cys Cys Ala Ala Ala Gly Gly Ala Cys Ala Ala Cys Cys225 230 235 240Gly Ala Ala Ala Ala Ala Thr Ala Ala Ala Cys Gly Thr Cys Cys Thr 245 250 255Ala Gly Gly Thr Cys Ala Gly Ala Ala Ala Cys Thr Gly Gly Thr Gly 260 265 270Cys Thr Cys Ala Ala Cys Cys Thr Ala Gly Cys Thr Cys Cys Ala Ala 275 280 285Ala Gly Cys Ala Thr Gly Cys Ala Gly Gly Gly Ala Gly Ala Ala Ala 290 295 300Gly Thr Gly Ala Gly Ala Ala Gly Gly Gly Ala Cys Ala Ala Gly Cys305 310 315 320Thr Ala Ala Ala Cys Gly Ala Gly Ala Gly Gly Thr Thr Cys Thr Thr 325 330 335Gly Gly Ala Ala Thr Thr Gly Gly Gly Thr Gly Cys Thr Gly Thr Cys 340 345 350Thr Thr Gly Gly Ala Thr Cys Cys Gly Gly Gly Gly Ala Ala Ala Ala 355 360 365Cys Ala Cys Cys Thr Ala Ala Ala Ala Thr Cys Gly Ala Cys Ala Ala 370 375 380Ala Thr Gly Thr Gly Cys Thr Ala Thr Ala Thr Thr Ala Ala Ala Thr385 390 395 400Gly Ala Thr Gly Cys Thr Ala Thr Cys Cys Gly Thr Gly Cys Ala Gly 405 410 415Thr Ala Ala Cys Thr Gly Ala Ala Thr Thr Gly Cys Gly Thr Ala Gly 420 425 430Thr Gly Ala Gly Gly Cys Ala Gly Ala Gly Ala Ala Gly Thr Thr Gly 435 440 445Ala Ala Gly Gly Ala Thr Thr Cys Ala Ala Ala Cys Gly Ala Cys Thr 450 455 460Cys Thr Cys Thr Cys Cys Ala Ala Gly Ala Gly Ala Ala Gly Ala Thr465 470 475 480Thr Ala Gly Ala Gly Ala Gly Cys Thr Ala Ala Ala Gly Gly Cys Thr 485 490 495Gly Ala Gly Ala Ala Gly Ala Ala Thr Gly Ala Gly Cys Thr Gly Cys 500 505 510Gly Ala Gly Ala Thr Gly Ala Gly Ala Ala Gly Cys Ala Ala Ala Ala 515 520 525Gly Thr Thr Gly Ala Ala Gly Gly Cys Gly Gly Ala Gly Ala Ala Ala 530 535 540Gly Ala Gly Ala Gly Cys Cys Thr Gly Gly Ala Gly Cys Ala Gly Cys545 550 555 560Ala Gly Ala Thr Thr Ala Ala Gly Thr Thr Cys Ala Thr Gly Ala Ala 565 570 575Thr Gly Cys Cys Cys Gly Thr Cys Ala Gly 580 58591651DNAHordeum vulgare 91cggcacgagg ccaccccggc tcccaatccc tgtgctgaaa taatagctct gtttcggtgg 60actgggacca tgccaaagga caaccgaaaa ataaacgtcc tagatcagaa actggtgctc 120aacctagctc caaagcatgc agggagaaag ttagaaggga caagctaaat gagaggttct 180tggaattggg tgctgtcttg gacccgggga aaacacctaa aatcgacaaa tgtgctatat 240taaatgatgc tatccgtgcg gtaactgaat tgcgtagtga agcagagaag ttgaaggatt 300caaatgagtc tctccaagag aagattagag agctgaaggc tgagaagaat gagctgcgag 360atgagaagca aaagctgaag gcggaaaaag agagcctgga gcagcagatt aagttcatga 420atgcccgtca gagactcgta ccacaccctt ctgtcatccc agctactgca ttcgctgccg 480cccaaggcca agcggcaggg cataagctta tgatgcctgt aatgagctac ccaggatttc 540ccatgtggca gttcatgccg ccttcagatg ttgatacctc ggatgaccct aagtcatgcc 600ctcctgttgc ataagccagc gaaaatcatt tgcctcatct atctcatggg g 65192590DNATriticum aestivummisc_feature(21)..(21)n is a, c, g, or t 92gagggactgc cccctcatgg nacgaccttg ctgccgccga cttcgccgcg gcatccgcag 60gaggcttcta ctggaccccg ccgatgcagc cgcagatgca cactcttgcg caggccgtct 120ccgccacccc ggctcccaat ccctgtgctg aaatcaatag ctctgtttcg gtggactggg 180accatgccaa aggacaaccg aaaaataaac gtcctaggtc agaaactggt gctcaaccta 240gctccaaagc atgcagggag aaagtgagaa gggacaagct aaacgagagg ttcttggaat 300tgggtgctgt cttggatccg gggaaaacac ctaaaatcga caaatgtgct atattaaatg 360atgctatccg tgcggtaact gaattgcgta gtgaagcaga gaagttgaag gattccaatg 420agtctctcca agagaagatt agagagctaa aggctgagaa gaatgagcta cgagatgaga 480agcaaaagtt gaaggcggag aaagagagcc tggagcagca gattaagttc atgaatgccc 540gtcagagcct cgtaccacac ccttctgtca tcccagctgc tgcattcgct 59093845DNAOryza sativa 93ctttttttaa ttggacagat ggaatttatt tactaatatg caatatacaa cggtgtcatc 60tttcgtcagt tcacatgtca ccggttgata cttcgataca gcgaagcttc ggtttaatcc 120tgggtttagc taggtagtaa acactgacga ccaaactggg cttgaatgga aggcagtttc 180acctctttcc tacaaggcga ggcaaattga tctttgcttt gacttatgca acaggagggc 240atgacttggt gtcatctgtg gtatcaacat cagaaggcgg catgaactgc cacatcggaa 300atcctgggta gccaatcaca ggcatcatca gcttctgccc ggcagcttga ccttgaggag 360cagtgaatgc actggctggg ataaccggag ggtgtggtac gaagcttggt cgagcattca 420ggaactttat ctgctgctcc aggctatctt tctctgcctt cagcttttgc ttctcatcac 480gcagctcgtt tttctcagcc ttcaactctt tgattttctc ttggagactc tcatttgatt 540ccttcaactt ctgtgcctca ctacgcagct cagccattac acgaatagca tcgttcaata 600tagacgattt gtccattttg ggtgtcttcc ctggttccag gacagcaccc agttccaaga 660acctctcgtt caacttgtcc cttcttactt tttccctgca tgctttggag cttggtcgag 720tgccactttc tgatctcgga cgtttatttg ttggctgttc tttgccatgt tcacagtcca 780cagagccatt gatttcagca ctagtgaaaa aaataaactt gtacttcagt atgccatatg 840tcatg 84594730DNATriticum aestivummisc_feature(32)..(32)n is a, c, g, or t 94ggtatttatt tactaacaat atacacggtt tncaagataa tnaatgttcc cgggtgatac 60aacgacgacg cttcagttcg gttcttgatt taactacata tagtaacact aaacggcttc 120tgcatagtaa cactgacgac taagatatac tttaacggac ggctttttag ccatccttcc 180ccatgagata gatgaggcaa atgatttttg ctggcttatg caaccggagg gcatgacttg 240gggtcatcgg aggtatcaac atctgaaggc ggcatgaact gccacatggg aaatcctggg 300tagctcatca caggcatcat cagcttgtgc cctgccgctt ggccttgggc ggcagcgaat 360gcagcagctg ggatgacaga agggtgtggt acgaggctct gacgggcatt catgaactta 420atctgctgct ccaggctctc tttctccgcc ttcaactttt gcttctcatc tcgtagctca 480ttcttctcag cctttagctc tctaatcttc tcttggagag actcattgga atccttcaac 540ttctctgctt cactacgcaa ttcagttacc gcacggatag catcatttaa tatagcacat 600ttgtcgattt taggtgtttt ccccggatcc aagacagcac ccaattccaa gaacctctcg 660tttagcttgt cccttctcac tttctccctg catgctttgg agctaggttg agcaccagtt 720tctgacctag 73095770DNASorghum bicolormisc_feature(559)..(559)n is a, c, g, or t 95cctactggac gaccgcttcc gccccagtcc ccaccgccct cgaccccgat tcccccaatc 60cctgccgcga ccgctgaacc ctagcctact ccggccatct gccgctggcc ccggcgatcc 120cccgccatgg cctcccccga gggaaccacg tgggtcttcg actgtcccct catggacgac 180ctcgcggtgg ccgccgactt cgcggcagcc cccgcggggg gatttttctg ggcagcgccg 240ccgtcgctac agccgcaggt ggtgcaggcg ccggtccagt ctgtcgttgc cgcgtcggct 300cccaacccat gtgtggaaat cagtagctct gtggactgtg gtcagggaaa agaacagcca 360acaaataaac gtcctaggtc agaaagtacc gcagaaccaa gcacaaaagc atccagggag 420aaaattagaa gggataagct gaacgagaga ttcctggaat tgggtgccat tttggagcca 480gggaaaactc ctaaaatgga caagtcagct atattaaatg atgctattcg tgtagtaggt 540gaattgcgta gcgaagcana agagctcaag gattcaaatg agagcctaca agagaagatt 600aaagagctaa aggctgagaa gaatgagctg cgagacgaga agcaaaggct gaaggccgag 660aaggagagcc tggagcagca gatcaagttc ctgaatgccc gcccaagtct ggtaccacac 720cacccagtga tctcagcctc tgccttcact gctccccaag ggccggcagt 77096761DNASorghum bicolor 96ggactgtggt cagggaaaag aacagccaac aaataaacgt cctaggtcag aaagtaccgc 60agaaccaagc acaaaagcat ccagggagaa aattagaagg gataagctga acgagagatt 120cctggaattg ggtgccattt tggacccagg gaaaactcct aaaatggaca agtcagctat 180attaaatgat gctattcgtg tagtaggtga attgcgtagc gaagcaaaag agttcaagga 240ttcaaatgag agcctacaag agaagattaa agagctaaag gctgagaaga atgagttgcg 300agacgagaag caaaggctga aggccgagaa ggagagcctg gagcagcaga tcaagttcct 360gaatgcccgc ccaagtctgg taccacacca cccagtgatt tcagcctctg ccttcactgc 420tccccagggg ccggcagtcg ccgggcacaa gctgatgatg cctgtgcttg gctaccctgg 480attcccgatg tggcagttca tgccgccttc tgatgttgac acctctgatg accccaagtc 540ttgcccacct gtggcgtaag caagtgaaga ggcgatgctg ccctccattg attcaagtct 600agatcgtgat cagtctgcag tgttgttggt gtagttgact ccactctcca gaatggaagg 660gaaggttata tgtgtcggat ggtgacatgg ggtgatctga tgaccccttt gtatattata 720tggtaaatga ataaattccg tgaccagttg caaatgagga t 76197630DNASorghum bicolor 97ggggtcatca gaggtgtcaa catcagaagg cggcatgaac tgccacatcg ggaatccagg 60gtagccaagc acaggcatca tcagcttgtg cccggcgact gccggcccct ggggagcagt 120gaaggcagag gctgagatca ctgggtggtg tggtaccaga cttgggcggg cattcaggaa 180cttgatctgc tgctccaggc tctccttctc ggccttcagc ctttgcttct cgtctcgcag 240ctcattcttc tcagccttta gctctttaat cttctcttgt aggctctcat ttgaatcctt 300gagctctttt gcttcgctac gcaattcacc tactacacga atagcatcat ttaatatagc 360tgacttgtcc attttaggag ttttccctgg ctccaaaatg gcacccaatt ccaggaatct 420ctcgttcagc ttatcccttc taattttctc cctggatgct tttgtgcttg gttctgcggt 480actttctgac ctaggacgtt

tatttgttgg ctgttctttt ccctgaccac agtccacaga 540gctactgatt tccacacatg ggttgggagc cgacgcggca acgacagact ggaccggcgc 600ctgcaccacc tgcggcgtag cgacggcggc 63098639DNAZea mays 98accctagcat actccggcat ctgctgcggc cccggcgatc ccccgccatg gcctcccccg 60agggcacaac gtgggtcttc gactgtcccc ttatggacga cctcgcggtc gccgccgact 120tcgcggcagc ccccgcggga ggatttttct gggcagcgcc gccgtcgctg cagccgcagg 180cgccagtgca gtctgtcgtt gccgcgtcgg ctcccaaccc atgtatggaa atcagtagct 240ctgtggactg tggtcaggaa aaagaacagc caacaaataa acgtccaagg tcagaaagta 300ctacagaatc aagcacaaaa gcatccaggg agaaaattag aagggacaag ctgaacgaga 360gattcttgga attgggtgcc attttggagc cagggaaaac tcctaaaatg gacaaaacag 420ctatattgag tgatgctatt cgtgtagtag gtgaattgcg tagtgaagca aaaaagctca 480aggattcaaa tgagaatctc caagagaaga ttaaagagct gaaggccgag aagaatgagc 540tgcgagacga gaagcaaagg ctgaaggccg agaaggagag cctggagcag cagatcaagt 600tcctgaatgc ccggccaagc ctcgtaccac accacccag 63999633DNASorghum bicolor 99gaggtgtcaa catcagaagg cggcatgaac tgccacatcg ggaatccagg gtagccaagc 60acaggcatca tcagcttgtg cccggcgact gccggcccct gggggagcag tgaaggcaga 120ggctgagatc actgggtggt gtggtaccag acttgggcgg gcattcagga acttgatctg 180ctgctccagg ctctccttct cggccttcag cctttgcttc tcgtctcgca gctcattctt 240ctcagccttt agctctttaa tcttctcttg taggctctca tttgaatcct tgagctcttt 300tgcttcgcta cgcaattcac ctactacacg aatagcatca tttaatatag ctgacttgtc 360cattttagga gttttccctg gctccaaaat ggcacccaat tccaggaatc tctcgttcag 420cttatccctt ctaattttct ccctggatgc ttttgtgctt ggttctgcgg tactttctga 480cctaggacgt ttatttgttg gctgttcttt tccctgacca cagtccacag agctactgat 540ttccacacat gggttgggag ccgacgcggc aacgacagac tggaccggcg cctgcaccac 600ctgcggctgt agcgacggcg gcgggggggc ccg 633100622DNAZea maysmisc_feature(591)..(591)n is a, c, g, or t 100gcacgagctc gcggtcgccg ccgacttcgc ggcagccccc gcgggaggat ttttctgggc 60agcgccgccg tcgctgcagc cgcaggcgcc agtgcagtct gtcgttgccg cgtcggctcc 120caacccatgt atggaaatca gtagctctgt ggactgtggt caggaaaaag aacagccaac 180aaataaacgt ccaaggtcag aaagtactac agaatcaagc acaaaagcat ccagggagaa 240aattagaagg gacaagctga acgagagatt cttggaattg ggtgccattt tggagccagg 300gaaaactcct aaaatggaca aaacagctat attgagtgat gctattcgtg tagtaggtga 360attgcgtagt gaagcaaaaa agctcaagga ttcaaatgag aatctccaag agaagattaa 420agagctgaag gccgagaaga atgagctgcg agacgagaag caaaggctga aggccgagaa 480ggagagcctg gagcagcaga tcaagttcct gaatgcccgg ccaagcctcg taccacacca 540cccagtgatc ccagcctctg cgttccctgc tccccagggg ccagcaaccg ncgccaggca 600caagctgatg atgcctgtga tt 622101705DNASaccharum officinarum 101cgtagtgacc gggtcgaccc acgcgtccgc cgccctcgac cccgaatccc ccaatccctg 60acgtgaccgc tgaaccctag cctactccgg ccatctgccg ctggccccgg cgatcccccg 120ccatggcctc ccccgaggga accacgtggg tcttcgactg tccccttatg gacgacctcg 180cggtggccgc tgacttcgcg gcagcccccg cgggggggtt tttctgggcg gcgccgccgt 240cgctgcagcc gcaggtggtg caggcgccgg tgcagtctgt cgttgccgcg tcggctccta 300accccccatg tgtggaaatt agtagctctg tggattgtgg tcagggaaaa gaacaaccaa 360caaataaacg tcctaggtca gaaagtactg cagaaccaag cacaaaagca tccagggaga 420aaattagaag ggacaagctg aacaagagat tcctggaatg gggtgccatt ttggagccag 480gggaaactcc taaaatggac aaatcagcta tattgaatga tgctattcgt gcagtaggtg 540aattgcgtag cgaagcaaaa aagctgaagg actcaaatga gagtttgcag gagaagatta 600aagagctgaa ggctgagaag aatgagtcgc gagacgagaa gcaaaggctg aaagccgaga 660acgagagcct ggagcagcag atcaagttcc tgaatgcccg cccaa 705102624DNASaccharum officinarummisc_feature(598)..(598)n is a, c, g, or t 102attttgttta caatgccatg ttatttgttt tcttgctgct tattatggtg cattatcttg 60atatttggca tcctaacatt ggcttctatt ttagcagtgt ggaaattagt agctctgtgg 120attgtggtca gggaaaagaa caaccaacaa ataaacgtcc taggtcagaa agtactgcag 180aaccaagcac aaaagcatcc agggagaaaa ttagaaggga caagctgaac aagagattcc 240tggaattggg tgccattttg gagccagggg aaactcctaa aatggacaaa tcagctatat 300tgaatgatgc tattcgtgca gtaggtgaat tgcgtagcga agcaaaaaag ctgaaggact 360caaatgagag tttgcaggag aagattaaag agctgaaggc tgagaagaat gagttgcgag 420acgagaagca aaggctgaag gccgagaagg agaagcctga gcagcagatc aagttcctga 480atgcccgccc aagcctggta ccacaccact cggtgatccc agcctctgcc ttcgctgctc 540cccaggggcc ggcagcagct gggcacaaac tgatgctgcc tgtgcttggc taccctgnat 600tcccaatgtg gcagttcatg cccc 624103671DNASorghum bicolor 103agggataagc tgaacgagag attcctggaa ttgggtgcca ttttggagcc agggaaaact 60cctaaaatgg acaagtcagc tatattaaat gatgctattc gtgtagtagg tgaattgcgt 120agcgaagcaa aagagctcaa ggattcaaat gagagcctac aagagaagat taaagagcta 180aaggctgaga agaatgagtt gcgagacgag aagcaaaggc tgaaggccga gaaggagagc 240ctggagcagc agatcaagtt cctgaatgcc cgcccaagtc tggtaccaca ccaccccagt 300gatttcagct tctgccttca ttgctcccca ggggccggca gtcgccgggc acaagctgat 360gatgcctgtg cttggctacc ctggattccc gatgtggcag ttcatgccgc cttctgatgt 420tgacacctct gatgacccca agtcttgccc acctgtggcg taagcaagtg aagaggcgat 480gctgccctcc attgattcaa gtctagatcg tgatcagtct gcagtgttgt tggtgtagtt 540gactccactc tccagaatgg aagggaaggt tatatgtgtc ggatggtgac atggggtgat 600ctgatgaccc ctttgtatat tatatggtaa atgaataaat tccgtgacca gttgcaaatg 660aggattagca g 671104610DNATriticum aestivummisc_feature(592)..(592)n is a, c, g, or t 104tggtatttat ttactaacaa tatacaacgg tttcacaaga taatcaaatg ttcccgggtg 60atacaacgac gacgcttcag ttcggttctt gatttaacta catatagtaa cactaaacgg 120cttctgcata gtaacactga cgactaagat atactttaac ggacggcttt ttagccatcc 180ttccccatga gatagatgag gcaaatgatt tttgctggct tatgcaaccg gagggcatga 240cttggggtca tcggaggtat caacatctga aggcggcatg aactgccaca tgggaaatcc 300tgggtagctc atcacaggca tcatcagctt gtgccctgcc gcttggcctt gggcggcagc 360gaatgcagca gctgggatga cagaagggtg tggtacgagg ctctgacggg cattcatgaa 420cttaatctgc tgctccaggc tctctttctc cgccttcaac ttttgcttct catctcgtag 480ctcattcttc tcagccttta gctctctaat cttctcttgg agagactcat tggaatcctt 540caacttctct gcttcactac gcaattcagt taccgcacgg atagcatcat tnaatatagc 600acatttgncg 610105628DNATriticum aestivummisc_feature(47)..(47)n is a, c, g, or t 105ggttagatct ccccaaggat tccggcagca ataaacgctt aaggtcngag ccctgtggta 60ggccgacatc taaggcttgt agggaaaaag tgagaagaga caagctgaat gacaggttcc 120ttgaattggg tactacattg gatcctggta agccagtaaa agctgacaaa gctgctatcc 180tgagtgatgc gactcgcatg gttactcagc ttcgtgctga agcgcagcag ctaaaggata 240ctaatggaag tctagaagac aagattaaag agttgaaggc agagaaggat gaacttcgtg 300atgagaagca gaagctgaaa ctagagaaag agacattaga gcaccagatg aaacttttga 360cggcaactcc agcctatatg cctcatccta ctatgatgcc ctccccgttc gctcaggctc 420cgatggctcc cttccatgca cagggacaag ctctaggaca gaaactgatg atgccctttg 480ttggttaccc aggatatccg atgtggcagt tgatgccgcc ctctgaagtc gacacctcaa 540aggacagcga agcatgcccg cctgttgcgt gatatgcttg gaccgcttaa atcgcatgaa 600ctcatggtaa cctaaaacag catagttg 6281061136DNATriticum aestivummisc_feature(9)..(9)n is a, c, g, or t 106ggtgatacna gagcggccgc cctttttttt ttttttagtt agaaaaggaa agcatcccat 60tcgaattgtg attcgtttct aggcggagat cagaacaaga acgagcattc tttctttctc 120gagaaaaaat gtattataca gtggacgaag ggtagtagac tctatagagc gcacaactgc 180cagcattctc ctcatcaaca aaaactaccg catgagaaca atagttaatc tgaatcagag 240agactatgcg caaccaatgg atcaaacaac tatgctgttt taggtgacca ggggttcatg 300cgatttaagc ggtccaagca tatcacgcaa caggcgggca tgcttcgctg tcctttgagg 360tgtcgacttc agagggcggc atcaactgcc acatcggata tcctgggtaa ccaacaaagg 420gcatcatcag tttctgtcct agagcttgtc cctgtgcatg gaagggagcc atcggagcct 480gagcgaacgg ggagggcatc atagtaggat gaggcatata ggctggagtt gccgtcaaaa 540gtttcatctg gtgctctaat gtctctttct ctagtttcag cttctgcttc tcatcacgaa 600gttcatcctt ctctgccttc aactctttaa tcttgtcttc tagacttcca ttagtatcct 660ttagctgctg cgcttcagca cgaagctgag taaccatgcg agtcgcatca ctcaggatag 720cagctttgtc agcttttact ggcttaccag gatccaatgt agtacccaat tcaaggaacc 780tgtcattcag cttgtctctt ctcacttttt ccctacaagc cttagatgtt ggcctaccaa 840aagggctctg acttaagcgg ttattggtgc cggaatcctt ggggaaaact aaattggtca 900attgggtgcc taattcccca ctgggattgg accaagaaaa cgatgctttc ccggggaaaa 960cgccccaagc agcggccggg aggtccttca agattccgca atcgaggaac caactgtctc 1020cccccttcgg tcggagggaa aaaaattggg gccgggggaa cttacctccc gtttgtgctc 1080cagcggtccc ccaaaggggg gggggcaatt ttccgcccca tcttgtgttg ggtgtt 1136107646DNAZea maysmisc_feature(614)..(614)n is a, c, g, or t 107tttttttttt ttttttgccc gcggaattta tttataccat accatatata atatacaaag 60gggtcatcag atcaccccat gtcaccgtcc gacacatgta acatcccctt cagttctgga 120atcaactaca ccaacaacac tgcggaccga tcaagatttg aatcaatgga aggcagcatc 180gcctctttcc aacaggattt cgcacggctt ctacgccaca ggaggacaag acctagggtc 240atcagaggtg tcaacatctg aaggcggcat gaactgccac atcgggaatc cagggtagcc 300aatcacaggc atcatcagct tgtgcctggc ggcggctgct ggcccctggg gagcagggaa 360cgcagaggct gggatcactg ggtggtgtgg tacgaggctt ggccgggcat tcaggaactt 420gatctgctgc tccaggctct ccttctcggc cttcagcctt tgcttctcgt ctcgcagctc 480attcttctcg gccttcagct ctttaatctt ctcttggaga ttctcatttg aatccttgag 540cttttttgct tcactacgca attcacctac tacacgaata gcatcactca atatagctgt 600tttgtccatt ttangagttt tccctggctc caaaatggca cccaat 646108916DNAZea mays 108gcacgcggta aggtctctct tcggcggcgg cgatgtctct cccccctgac ccggcgggcg 60gcggcgccgg caccggcacc ggcgacgact ggttcctcga ctgcggcatc ctcgacgacc 120tcccggccgc ggcctgcggg gccttcccgt gggacgcgtc cccgtcttct tccaacccca 180gtgtggaagt gggcagctat gtgaacacca atgatgtttt caaggagccc aatgatgtct 240tcaaggagcc tggcagcaat aagcgtttga ggtcaggatc caatgatgtg catgtgccaa 300catctaaagc ttctagggaa aaaatgagga ggaacaagct gaatgacagg ttccttgaat 360tggggtctac attagaacca gggaagccag taaaagctga caaagctgct atcctaagtg 420atgctactcg catggttatt cagcttcgtt cagaagcaca gcagctgaag gaaactaatg 480gtagtcttga agaaaagatc aaagaactca aggccgagaa ggatgaactt cgtgatgaga 540agcagaagct gaaattggag aaggagagtt tagaacacca gatgaagctg atgacatcga 600ctccaaccta catgcctcat ccaaccctga tgccggcgcc tttccctcag gcacccctag 660cgccgttcca tgcccagggg caagctgcag ggcagaagct gatgatgccc tttgtcagct 720atccggggta cccaatgtgg cagttcatgc cgccttcaga ggtcgacacc tcgaaggaca 780gtgaagcgtg ccctcctgtt gcataatcgc ttcgactggc ggctggtcgt gctcacacca 840tgcgaattag tcgcaactga agcccccccc ctagctgtcg atccattgat tggctataac 900tgctgttgtt atattt 916109778DNASorghum bicolor 109gaattttgtt atagtcccca aaaggtctct tcggcggcgg ctgcggcggt ggcgatgtct 60ctccccccgg acggcggcgg tgtcaccggc gacgactggt tcctcgactg cggcatcctc 120gacgacctcc cgtgggacgc gtccccgtcg tcttcttccc cagtgtggaa gtgggcagct 180atgtgaacac aaatgatgtc ttcaaggagc ccaatgatgt cttcaaggag cctggcagca 240ataaacgttt aaggtcagga tccaatgatg tgccaacatc taaagcttct agggaaaaaa 300tgaggaggaa caaactgaat gataggtttc ttgaattggg gtctacatta gaaccaggga 360agccagtaaa agctgacaag gctgctatcc taagtgatgc tactcgcatg gttattcagc 420ttcgttcaga agcacagcag ctcaaggaaa ctaatggtag ccttgaagaa aagattaaag 480aactaaaggc tgagaaggat gagcttcgtg atgagaagca gaagttgaaa ttagagaagg 540agagtttaga gcaccagatg aagcttatga cctcaactcc agcctacatg cctcatccga 600ccctgatgcc agcgcctttc cctcaggcgc ccttagcacc attccatgcc caggggcaag 660ctgcagggca gaagctgatg atgccctttg tcagctatcc ggggtaccca atgtggcagt 720tcatgccgcc atcagaagtt gacacctcga aggacagcga agcgtgccct cctgttgc 778110832DNASorghum bicolor 110caaggagcct ggcagcaata aacgtttaag gtcaggatcc aatgatgtgc caacatctaa 60agcttctagg gaaaaaatga ggaggaacaa actgaatgat aggtttcttg aattggggtc 120tacattagaa ccagggaagc cagtaaaagc tgacaaggct gctatcctaa gtgatgctac 180tcgcatggtt attcagcttc gttcagaagc acagcagctc aaggaaacta atggtagcct 240tgaagaaaag attaaagaac taaaggctga gaaggatgag cttcgtgatg agaagcagaa 300gttgaaatta gagaaggaga gtttagagca ccagatgaag cttatgacct caactccagc 360ctacatgcct catccgaccc tgatgccagc gcctttccct caggcgccct tagcaccatt 420ccatgcccag gggcaagctg cagggcagaa gctgatgatg ccctttgtca gctatccggg 480gtacccaatg tggcagttca tgccgccatc agaagttgac acctcgaagg acagcgaagc 540gtgccctcct gttgcgtaat tgcttcggtc gactggccac gcttgcacca tgtgaattaa 600tcacaactga agcccccccc tagtcgttga tccattgatt gggtataact atttgttctt 660atgtggtagt tcattggtaa cgaagtaaag ctgctgatgt aagcgtccta ctatatagag 720gctactactg ctgccccttc aatggctgta caatttttgt ggagaaaaga agaatgttcg 780ttcttgttct ccatagcaac aatgtgacgc tatctatatg tcatatatat tc 832111636DNAHordeum vulgaremisc_feature(15)..(15)n is a, c, g, or t 111gaaacgctta aggtnagggc cctgtggtag gccaacatct aaggcttgta gggaaaaagt 60gagaagagac aagctgaatg acaggttcct tgaattgggt actacattgg atcctggtaa 120gccagtaaaa gctgacaaag ctgctatcct aagtgatgcg actcgcatgg ttactcagct 180tcgtgctgaa gcgaagcagc taaaggatac caatggaagt ctagaagaca agattaaaga 240gttgaaggca gagaaggatg aacttcgtga cgagaagcag aagctgaaat tagagaaaga 300gacattagag caccagatga aactattgac tgcaactcca gcctatatgc ctcatcctac 360catgatgcac tccccatttg ctcaggcgcc aatggctccc ttccatgcac aggggcacgc 420ttcagcacag aaactgatga tgccctttgt tggttacccg ggatatccga tgtggcagtt 480gatgccgccc tccgaagtcg acacctcaaa ggacagcgaa gcttgcccgc ctgttgcgtg 540atgcttggac cgtttaaatc acatgaactc atggtaacct taaacagcgt agttgtttga 600tccattggtt gcgcatagtc tctctgattc agatta 636112644DNASorghum bicolor 112agtcagctat attaaatgat gctattcgtg tagtaggtga attgcgtagc gaagcaaaag 60agctcaagga ttcaaatgag agcctacaag agaagattaa agagctaaag gctgagaaga 120atgagctgcg agacgagaag caaaggctga aggccgagaa ggagagcctg gagcagcaga 180tcaagttcct gaatgcccgc ccaagtctgg taccacacca cccagtgatc tcagcctctg 240ccttcactgc tccccagggg ccggcagtcg ccgggcacaa gctgatgatg cctgtgcttg 300gctaccctgg attcccgatg tggcagttca tgccgccttc tgatgttgac acctctgatg 360accccaagtc ttgcccacct gtggcgtaag caagtgaaga ggcgatgctg ccctccattg 420attcaagtct agatcgtgat cagtctgcag tgttgttggt gtagttgact ccactctcca 480gaatggaagg gaaggttata tgtgtcggat ggtgacatgg ggtgatctga tgaccccttt 540gtatattata tggtaaatga ataaattccg tgaccagttg caaatgagga ttagcagact 600agctcatgtc tattcctgct ttttgtcgta taaaccacgt tgtg 644113731DNAZea mays 113tttttttttt ttttttccag ccagcgtgga tataaacatg caaaacattg tcttacacta 60tagactgtac tgtttacaga gtaaaattta aaatagggag taagatagag aataggatag 120agaggctgct ggagatagcc taagaacatc agttataccc catcaatgga tcgacaacga 180caactagttg cggctaatcc acatggtgtg agcaggacca gccccatcaa ggtgatcatg 240cgacaggagg gcacgcctcg ctgtccttcg aggtgtcgac ctctgaaggc ggcatgaact 300gccacattgg gtaccctggg tagccgacga agggcatcat cagcttctgc cctgcagctt 360ggccctgggc atggaatgga gctaggggcg cctgggcgaa aggcgccggc atcagggtcg 420gatggggcat gtaggctgga gccgatgcca tcagcttcat ctggtgctct agactctcct 480tctccagttt cagtttctgc ttctcgtcgc gaagctcgtc cttctcggcc tttagttctt 540taatcttttc ttcgaggctg ccattagtct ccttcagttg ctgtgattct gaacggagct 600gaataaccat gcgagtagca tcgcttagga tggcagcttt gtcagctttc actggcttcc 660caggttctaa tgcagacccc agttcaagaa acctgtcatt cagcttgttc ctcctcattc 720tttccctgca a 731114572DNAZea mays 114tataccatac catatataat atacaaaggg gtcatcagat caccccatgt caccgtccga 60cacatgtaac atccccttca gttctgcgaa tcaactacac caacaacact gcggaccgat 120caagatttga atcaagggaa ggcagcatcg cctctttcca acaggatttc gcacggcttc 180tacgccacag gaggacaaga cctagggtca tcagaggtgt caacatctga aggcggcatg 240aactgccaca tcgggaatcc agggtagcca atcacaggca tcatcagctt gtgcctggcg 300gcggctgctg gcccctgggg agcagggaac gcagaggctg ggatcactgg gcggtgtggt 360acgaggcttg gccgggcatt caggaacttg atctgctgct ccaggctctc cttctcggcc 420ttcagccttt gcttctcgtc tcgcagctca ttcttctcgg ccttcagctc tttaatcttc 480tcttggagat tctcatttga atccttgagc ttttttgctt cactacgcaa ttcacctact 540acacgaatag catcactcaa tatagctgtt tt 572115705DNAArabidopsis thaliana 115atggtgtcac ccgaaaacgc taattggatt tgtgacttga tcgatgctga ttacggaagt 60ttcacaatcc aaggtcctgg tttctcttgg cctgttcagc aacctattgg tgtttcttct 120aactccagtg ctggagttga tggctcggct ggaaactcag aagctagcaa agaacctgga 180tccaaaaaga gggggagatg tgaatcatcc tctgccacta gctcgaaagc atgtagagag 240aagcagcgac gggacaggtt gaatgacaag tttatggaat tgggtgcaat tttggagcct 300ggaaatcctc ccaaaacaga caaggctgct atcttggttg atgctgtccg catggtgaca 360cagctacggg gcgaggccca gaagctgaag gactccaatt caagtcttca ggacaaaatc 420aaagagttaa agactgagaa aaacgagctg cgagatgaga aacagaggct gaagacagag 480aaagaaaagc tggagcagca gctgaaagcc atgaatgctc ctcaaccaag ttttttccca 540gccccaccta tgatgcctac tgcttttgct tcagcgcaag gccaagctcc tggaaacaag 600atggtgccaa tcatcagtta cccaggagtt gccatgtggc agttcatgcc tcctgcttca 660gtcgatactt ctcaggatca tgtccttcgt cctcctgttg cttaa 705116705DNAArabidopsis thaliana 116atggtgtcac ccgaaaacgc taattggatt tgtgacttga tcgatgctga ttacggaagt 60ttcacaatcc aaggtcctgg tttctcttgg cctgttcagc aacctattgg tgtttcttct 120aactccagtg ctggagttga tggctcggct ggaaactcag aagctagcaa agaacctgga 180tccaaaaaga gggggagatg tgaatcatcc tctgccacta gctcgaaagc atgtagagag 240aagcagcgac gggacaggtt gaatgacaag tttatggaat tgggtgcaat tttggagcct 300ggaaatcctc ccaaaacaga caaggctgct atcttggttg atgctgtccg catggtgaca 360cagctacggg gcgaggccca gaagctgaag gactccaatt caagtcttca ggacaaaatc 420aaagagttaa agactgagaa aaacgagctg cgagatgaga aacagaggct gaagacagag 480aaagaaaagc tggagcagca gctgaaagcc attaatgctc ctcaaccaag ttttttccca 540gccccaccta tgatgcctac tgcttttgct tcagcgcaag gccaagctcc tggaaacaag 600atggtgccaa tcatcagtta cccaggagtt gccatgtggc agttcatgcc tcctgcttca 660gtcgatactt ctcaggatca tgtccttcgt cctcctgttg cttaa 705117681DNAArabidopsis thaliana 117atggtgtctc cggagaatac gaactggctt agtgattacc ctttgattga aggtgctttc 60tctgatcaga accccacttt cccttggcag atagatggct cagctactgt cagtgttgaa 120gtggatggct tcctttgtga tgcagatgtg

atcaaagaac caagttcaag gaagaggatc 180aaaactgaat cttgcactgg ttctaactcg aaagcttgta gggagaaaca aagacgtgat 240agactaaatg acaagtttac ggagttgagt tccgtattgg aacctgggag aactccaaaa 300acagacaagg ttgctattat caatgatgca attcgcatgg tgaatcaagc aagagatgaa 360gcgcagaaac taaaggactt gaactcaagc ctccaggaga aaatcaagga gttgaaggat 420gagaagaacg agctgcgtga tgagaaacag aagcttaagg tcgagaagga gagaatcgat 480cagcaactga aagctattaa gacacagcct cagcctcaac cttgtttctt accaaatccg 540caaacactct ctcaagctca agctcctgga agcaagcttg tccctttcac aacttatccc 600ggctttgcaa tgtggcaatt catgcctcct gctgctgttg atacctcaca ggaccatgtc 660cttcgtcctc cagttgctta a 681118876DNAArabidopsis thaliana 118atgcaaacaa atgaagataa catatttcag gattttgggt cttgtggtgt gaatctgatg 60cagccacaac aagaacaatt tgattctttt aatggaaatc ttgagcaagt ttgtagtagc 120tttagaggag gaaacaatgg agttgtttat agtagtagca ttggatcagc acaattggat 180ttggctgcat cgtttagtgg agttttgcag caagagacac atcaagtctg tggctttaga 240ggacaaaacg acgattctgc agtgcctcat ttgcagcagc aacaaggaca ggtgtttagt 300ggtgtagtgg aaatcaattc ttcgtcatct gttggagctg ttaaggaaga gtttgaggaa 360gaatgttcgg ggaagaggag acgaactgga tcatgtagca agccaggaac caaagcctgt 420cgcgagaaac taagaaggga aaagctaaat gacaagttca tggacttgag ctctgtttta 480gagcctggca ggactccaaa gacggataaa tcagctatac tcgacgatgc aatccgggtt 540gtgaatcagc ttagaggtga agctcatgag cttcaagaaa ccaaccaaaa gcttctagaa 600gagatcaaga gtctaaaggc ggataaaaac gagctacgag aggaaaagct ggtgttgaag 660gcggagaagg agaagatgga gcaacagtta aaatctatgg tggttccatc accaggtttc 720atgccctccc agcatccagc agctttccat tcccataaga tggcggtggc ttacccttac 780ggctactatc ctccaaacat gccaatgtgg tcacccttac ctcctgctga ccgtgatacg 840tctcgtgatc tcaaaaatct tcctcctgtt gcttaa 876119963DNAArabidopsis thaliana 119atgtatccat caatcgaaga cgatgatgat cttctcgctg ctctttgttt tgatcaaagc 60aatggagtag aagatcctta tggatatatg caaacaaatg aagataacat atttcaggat 120tttgggtctt gtggtgtgaa tctgatgcag ccacaacaag aacaatttga ttcttttaat 180ggaaatcttg agcaagtttg tagtagcttt agaggaggaa acaatggagt tgtttatagt 240agtagcattg gatcagcaca attggatttg gctgcatcgt ttagtggagt tttgcagcaa 300gagacacatc aagtctgtgg ctttagagga caaaacgacg attctgcagt gcctcatttg 360cagcagcaac aaggacaggt gtttagtggt gtagtggaaa tcaattcttc gtcatctgtt 420ggagctgtta aggaagagtt tgaggaagaa tgttcgggga agaggagacg aactggatca 480tgtagcaagc caggaaccaa agcctgtcgc gagaaactaa gaagggaaaa gctaaatgac 540aagttcatgg acttgagctc tgttttagag cctggcagga ctccaaagac ggataaatca 600gctatactcg acgatgcaat ccgggttgtg aatcagctta gaggtgaagc tcatgagctt 660caagaaacca accaaaagct tctagaagag atcaagagtc taaaggcgga taaaaacgag 720ctacgagagg aaaagctggt gttgaaggcg gagaaggaga agatggagca acagttaaaa 780tctatggtgg ttccatcacc aggtttcatg ccctcccagc atccagcagc tttccattcc 840cataagatgg cggtggctta cccttacggc tactatcctc caaacatgcc aatgtggtca 900cccttacctc ctgctgaccg tgatacgtct cgtgatctca aaaatcttcc tcctgttgct 960taa 963120852DNAArabidopsis thaliana 120atgtatcctt ctctcgacga tgatttcgtc tctgatttgt tttgcttcga tcaaagcaat 60ggagcagaac ttgatgatta cacacagttt ggtgtaaatt tgcagactga tcaagaggat 120acctttccag attttgtgtc atatggtgtg aatttgcagc aggagccaga tgaagtcttt 180agtattggag cttctcaatt ggatttgtcc tcgtataatg gagttttgtc gctagagcca 240gaacaggtgg ggcaacaaga ttgtgaagtt gtgcaggaag aagaagtaga gatcaattct 300ggttcatctg gtggagctgt taaggaagaa caggaacatt tagatgacga ttgctccaga 360aagcgggcaa ggactggatc gtgtagcaga ggaggaggaa ctaaagcgtg tcgtgaaagg 420ttgaggaggg agaagctaaa tgagaggttt atggatttga gctcggtttt ggagcctggg 480aggactccta agactgataa accggctata ctcgatgatg caatccgtat attgaatcaa 540cttagagatg aagctcttaa gcttgaagaa actaaccaga agcttttaga ggagatcaag 600agtctcaagg cggagaagaa cgagctgagg gaggaaaagc tggtgttgaa ggcggataaa 660gagaagacag aacaacagtt aaagtctatg acggctccat cttcagggtt catacctcat 720attccagctg catttaacca caacaaaatg gctgtttatc caagttacgg ttacatgcca 780atgtggcatt atatgcctca atccgttcgt gacacatctc gtgatcaaga actcaggcct 840cctgctgctt aa 852121834DNAArabidopsis thaliana 121atggatgtga atctttttgg tcatgatgac tcttgtagca atggagcaga acttgatgat 60tacacacagt ttggtgtaaa tttgcagact gatcaagagg atacctttcc agattttgtg 120tcatatggtg tgaatttgca gcaggagcca gatgaagtct ttagtattgg agcttctcaa 180ttggatttgt cctcgtataa tggagttttg tcgctagagc cagaacaggt ggggcaacaa 240gattgtgaag ttgtgcagga agaagaagta gagatcaatt ctggttcatc tggtggagct 300gttaaggaag aacaggaaca tttagatgac gattgctcca gaaagcgggc aaggactgga 360tcgtgtagca gaggaggagg aactaaagcg tgtcgtgaaa ggttgaggag ggagaagcta 420aatgagaggt ttatggattt gagctcggtt ttggagcctg ggaggactcc taagactgat 480aaaccggcta tactcgatga tgcaatccgt atattgaatc aacttagaga tgaagctctt 540aagcttgaag aaactaacca gaagctttta gaggagatca agagtctcaa ggcggagaag 600aacgagctga gggaggaaaa gctggtgttg aaggcggata aagagaagac agaacaacag 660ttaaagtcta tgacggctcc atcttcaggg ttcatacctc atattccagc tgcatttaac 720cacaacaaaa tggctgttta tccaagttac ggttacatgc caatgtggca ttatatgcct 780caatccgttc gtgacacatc tcgtgatcaa gaactcaggc ctcctgctgc ttaa 8341221014DNAArabidopsis thaliana 122atggggataa gagaaaatgg aataatgctt gtgagcagag agagagagcg agcgaggagg 60ctagagaatc gagaatcgat cttcgccgaa ccaccttgtc ttctcttagc tcatcgaatc 120tctccgtcgc cgtcgattct tcccgccgaa gaggaggtca tggacgtttc tgctagaaag 180tcacaaaaag ctgggcgcga aaagttgagg agggaaaaac tgaatgagca ttttgttgaa 240ctgggaaatg tactcgatcc agagagaccc aagaatgaca aagccacgat tctgactgat 300actgttcagt tgttgaaaga gctcacatct gaagtcaaca aactgaaatc tgagtacacc 360gcattgacag atgagtcccg cgagttgaca caggagaaaa acgacctgag agaagaaaag 420acatcgctga aatcagatat agagaatctc aatcttcaat accagcagag attaaggtca 480atgtctccat ggggagctgc gatggatcac acagtcatga tggctccacc accctccttt 540ccatacccta tgcctattgc tatgcctccc gggtcaatcc caatgcatcc atcaatgcca 600tcttacacat actttgggaa ccagaaccct agcatgatcc cagctccatg tcctacatac 660atgccctaca tgcctcctaa tacagtcgtt gagcaacaat ccgtgcacat tccacagaac 720cccggtaacc gttctcggga acctagagca aaggtttcaa gagagagcag atctgagaaa 780gcagaggact ccaacgaagt tgcaacacaa ctcgaattaa aaacccctgg atctacttct 840gataaggata cattgcaaag gccagagaag acaaagagat gtaagagaaa caacaacaac 900aactcaatag aagaaagctc tcattctagc aagtgttcat cttctccgag cgtacgagac 960cacagttctt ccagtagcgt agctggtggc caaaaacctg atgatgcaaa atga 1014123855DNAArabidopsis thaliana 123atggacgttt ctgctagaaa gtcacaaaaa gctgggcgcg aaaagttgag gagggaaaaa 60ctgaatgagc attttgttga actgggaaat gtactcgatc cagagagacc caagaatgac 120aaagccacga ttctgactga tactgttcag ttgttgaaag agctcacatc tgaagtcaac 180aaactgaaat ctgagtacac cgcattgaca gatgagtccc gcgagttgac acaggagaaa 240aacgacctga gagaagaaaa gacatcgctg aaatcagata tagagaatct caatcttcaa 300taccagcaga gattaaggtc aatgtctcca tggggagctg cgatggatca cacagtcatg 360atggctccac caccctcctt tccataccct atgcctattg ctatgcctcc cgggtcaatc 420ccaatgcatc catcaatgcc atcttacaca tactttggga accagaaccc tagcatgatc 480ccagctccat gtcctacata catgccctac atgcctccta atacagtcgt tgagcaacaa 540tccgtgcaca ttccacagaa ccccggtaac cgttctcggg aacctagagc aaaggtttca 600agagagagca gatctgagaa agcagaggac tccaacgaag ttgcaacaca actcgaatta 660aaaacccctg gatctacttc tgataaggat acattgcaaa ggccagagaa gacaaagaga 720tgtaagagaa acaacaacaa caactcaata gaagaaagct ctcattctag caagtgttca 780tcttctccga gcgtacgaga ccacagttct tccagtagcg tagctggtgg ccaaaaacct 840gatgatgcaa aatga 855124807DNAArabidopsis thaliana 124atggctgtgt catgtttatt catagtttcg tctaattaca gaggagctga gatggtggtg 60gaagtgaaga aggaagcagt ttgttcccag aaagcagagc gagagaagct tcgtagagat 120aagcttaagg aacagtttct tgagcttgga aatgcacttg atccgaatag gcctaagagt 180gacaaagcct cagttctcac tgatacaata caaatgctca aggatgtaat gaaccaagtt 240gatagactaa aagctgagta tgaaacacta tctcaagagt ctcgtgagct aattcaagag 300aagagtgagc tgagagagga gaaagcgact ttaaagtctg atatcgagat tcttaatgct 360caatatcagc atggaatcaa aaccatggtt ccatgggtac ctcattacag ttatcatatc 420cccttcgtag ccataactca gggtcagtcc agttttatac cttattcagc ctctgtcaat 480cctctaaccg aacaacaagc atcggttcag cagcattctt cttcttctgc cgatgcttca 540atgaaacaag attccaaaat caagccgtta gatttggatc tgatgatgaa cagtaaccat 600tcaggtcaag gaaatgatca aaaagatgat gttcgtttaa agctcgagct taaaatccat 660gcctcttctt tagctcaaca ggatgtttct ggaaaagaga agaaagtaag cttgacaacc 720actgcaagct catcgaatag ttactcatta tctcaagctg ttcaagatag ttcccccggt 780accgtaaatg acatgttgaa gccataa 807125861DNAArabidopsis thaliana 125atggatcaac caatgaaacc aaaaacttgc tctgaatctg attttgctga tgattcctct 60gcttcttctt cttcttcttc gggacaaaat ctcagaggag ctgagatggt ggtggaagtg 120aagaaggaag cagtttgttc ccagaaagca gagcgagaga agcttcgtag agataagctt 180aaggaacagt ttcttgagct tggaaatgca cttgatccga ataggcctaa gagtgacaaa 240gcctcagttc tcactgatac aatacaaatg ctcaaggatg taatgaacca agttgataga 300ctaaaagctg agtatgaaac actatctcaa gagtctcgtg agctaattca agagaagagt 360gagctgagag aggagaaagc gactttaaag tctgatatcg agattcttaa tgctcaatat 420cagcatagaa tcaaaaccat ggttccatgg gtacctcatt acagttatca tatccccttc 480gtagccataa ctcagggtca gtccagtttt ataccttatt cagcctctgt caatcctcta 540accgaacaac aagcatcggt tcagcagcat tcttcttctt ctgccgatgc ttcaatgaaa 600caagattcca aaatcaagcc gttagatttg gatctgatga tgaacagtaa ccattcaggt 660caaggaaatg atcaaaaaga tgatgttcgt ttaaagctcg agcttaaaat ccatgcctct 720tctttagctc aacaggatgt ttctggaaaa gagaagaaag taagcttgac aaccactgca 780agctcatcga atagttactc attatctcaa gctgttcaag atagttcccc cggtaccgta 840aatgacatgt tgaagccata a 861126807DNAArabidopsis thaliana 126atggctgtgt catgtttatt catagtttcg tctaattaca gaggagctga gatggtggtg 60gaagtgaaga aggaagcagt ttgttcccag aaagcagagc gagagaagct tcgtagagat 120aagcttaagg aacagtttct tgagcttgga aatgcacttg atccgaatag gcctaagagt 180gacaaagcct cagttctcac tgatacaata caaatgctca aggatgtaat gaaccaagtt 240gatagactaa aagctgagta tgaaacacta tctcaagagt ctcgtgagct aattcaagag 300aagagtgagc tgagagagga gaaagcgact ttaaagtctg atatcgagat tcttaatgct 360caatatcagc atagaatcaa aaccatggtt ccatgggtac ctcattacag ttatcatatc 420cccttcgtag ccataactca gggtcagtcc agttttatac cttattcagc ctctgtcaat 480cctctaaccg aacaacaagc atcggttcag cagcattctt cttcttctgc cgatgcttca 540atgaaacaag attccaaaat caagccgtta gatttggatc tgatgatgaa cagtaaccat 600tcaggtcaag gaaatgatca aaaagatgat gttcgtttaa agctcgagct taaaatccat 660gcctcttctt tagctcaaca ggatgtttct ggaaaagaga agaaagtaag cttgacaacc 720actgcaagct catcgaatag ttactcatta tctcaagctg ttcaagatag ttcccccggt 780accgtaaatg acatgttgaa gccataa 807127819DNAArabidopsis thaliana 127atggatcaac caatgaaacc aaaaacttgc tctgaatctg attttgctga tgattcctct 60gcttcttctt cttcttcttc gggacaaaat ctcagaggag ctgagatggt ggtggaagtg 120aagaaggaag cagtttgttc ccagaaagca gagcgagaga agcttcgtag agataagctt 180aaggaacagt ttcttgagct tggaaatgca cttgatccga ataggcctaa gagtgacaaa 240gcctcagttc tcactgatac aatacaaatg ctcaaggatg taatgaacca agttgataga 300ctaaaagctg agtatgaaac actatctcaa gagtctcgtg agctaattca agagaagagt 360gagctgagag aggagaaagc gactttaaag tctgatatcg agattcttaa tgctcaatat 420cagcatagaa tcaaaaccat ggttccatgg ggtcagtcca gttttatacc ttattcagcc 480tctgtcaatc ctctaaccga acaacaagca tcggttcagc agcattcttc ttcttctgcc 540gatgcttcaa tgaaacaaga ttccaaaatc aagccgttag atttggatct gatgatgaac 600agtaaccatt caggtcaagg aaatgatcaa aaagatgatg ttcgtttaaa gctcgagctt 660aaaatccatg cctcttcttt agctcaacag gtgagtgatc tcttcaatag ttttgccaac 720aagctctttc atggactgac cagagtttac ttccatgcag gatgtttctg gaaaagagaa 780gaaagtaagc ttgacaacca ctgcaagctc atcgaatag 819128799DNAVitis vinifera 128ccctaacgcg ctcctccctc tcccgggaaa ttgcaccggg cctcctcgat ttttcggagg 60ttccttcgag atggtttctc cagaagccac caattggctg tacgagtacg ggctcatcga 120ggacatccct gtccctgatt caaacttcgc taatacgaat tcagggttcg cctggactcc 180tgtgcaggcc ttgaacactt ctgctaatgt cagtggggaa attgatggtt catttgggga 240ctctgacggc attaaggaaa ctggatcaaa gaagagggtg agatctgaat catgtggtgc 300atctagctcg aaggcatgta gggagaagtt gcggagggac aggctaaatg acaagtttat 360ggaattgggt tctatcctgg agcctggaag gcctccaaaa acagacaagt cttctatttt 420gattgatgca gttcgaatgg taactcagtt acgaggtgag tcgcagaagt tgaaggactc 480aaattctagt ctccaggaga agattaaaga attgaaggct gagaagaatg agcttcgtga 540tgagaagcaa aggctaaagg ccgagaaaga gaaactggag cagcaactga aagcaatgaa 600tgctcaacct agtttcctgc ctcccgttcc ttcaatccct gctgcatttg cagctcaagg 660ccaagctggc ggcgacaagt tggttccatt catcggctac ccaggagttg ctatgtggca 720attcatgcca cctgctgcag ttgatacctc acaagatcat gtgctccgtc caccagttgc 780ttaaatcagc aactcaacc 799129839DNACitrus reticulata 129tttttttttt tttttttcaa tacagtcacc caccaatcat taaagacttc ggaaaaacta 60atacagtcag agaacaatga taaaaccaat acacttgttg ggtgcaaatt gcaaacccaa 120atcattgtag gttgcatact taagcaaccg gaggacggag tacatgatcc tgtgaggtat 180ccactgcagc aggaggcatg aattgccaca ttgcgactcc agggtagctg atgaaaggca 240tcagcttgtt tccaggtgct tggccttggg cagcaaatgc agcagggatt gcaggaggag 300gagttaagaa actgggttgt gtgctcatag ctttgagttg ctgctctatc ttctctttct 360ctgcctttag cctctgcttc tcatcacgaa gctcattctt ctcagccttc aactctttga 420tcttctcctg gagacttgaa tttgagtcct tcaacttctg ggcttcactg cgtaattgag 480tcaccatccg gacggcatca atcaaaatag ctgccttatc tgttttgggg ggccttccag 540gctctaagat agaggctaac tccacaaact tgtcattaag acgatcccta cgcaactttt 600ccctgcacgc cttggagcta gaagacccac atgattctga tctaaccctc ttttttgagc 660tttccttgag accatttgaa tccccaaatg cagaatcaat ttccacacag ccatttgacg 720gcccgttaat tggcggctga acggtccaag tgaaacccga agcggaaacg gagaaattgc 780catcagggac agtgatatcg tcgatcaagg ggtaatctaa tagccaattc gtattttcc 839130730DNAMedicago truncatula 130ttttccggga aattccccca ctatggtttc cccggaaaac accaattggc ttttcgatta 60ccctttgatt gatgaaattc ctgtttctgt tgatggctcc tttgccttca cgtggccccc 120acctcacctc tccaatggcg gtattgaaat ggatgatagc tctctagtgg attctgatgg 180tatcaaagaa cctggttcga agaagagagg tagatcagat tcatgtgctc cttccagctc 240taaggcatgt cgagagaagt tgcggaggga taggctgaac gacaagtttg ttgaattagg 300ctccatcttg gagcctggaa ggcctcctaa aacagacaag gcggcaattc tgattgatgc 360tgtccgaatg gtgacacagt tgcggggtga agcccaaaag ttgaaagact caaattcacg 420tcttcaagag aagattaaag agttaaaggt tgagaagaat gaactccgcg atgagaagca 480gaggcttaag gctgagaagg agaagttgga gcagcaggtg aaatcaatga acacccaacc 540cggtttcttg acacaccctc ctgcaatccc tgctgcattt gctcatcaag gccaagcccc 600aagcaacaag ttaatgcctt tcatgagtta tccaggagtt gccatgtggc aattcatgcc 660accagccgcc gtggatacct cacaggatca tgtactccgt ccaccagttg cctaaattgg 720cactgtacaa 730131811DNAVitis vinifera 131cttttctccc tcgagaaaac cccccttttc tctctcccca aacaccctaa cgcgctcctc 60cctctcccgg gaaattgcac cgggcctcct cgatttttcg gaggttcctt cgagatggtt 120tctccagaag ccaccaattg gctgtacgag tacgggctca tcgaggacat ccctgtccct 180gattcaaact tcgctaatac gaattcaggg ttcgcctgga ctcctgtgca ggccttgaac 240acttctgcta atgtcagtgg ggaaattgat ggttcatttg gggactctga cggcattaag 300gaaactggat caaagaagag ggtgagatct gaatcatgtg gtgcatctag ctcgaaggca 360tgtagggaga agttgcggag ggacaggcta aatgacaagt ttatggaatt gggttctatc 420ctggagcctg gaaggcctcc aaaaacagac aagtcttcta ttttgattga tgcagttcga 480atggtaactc agttacgagg tgagtcgcag aagttgaagg actcaaattc tagtctccag 540gagaagatta aagaattgaa ggctgagaag aatgagcttc gtgatgagaa gcaaaggcta 600aaggccgaga aagagaaact ggagcagcaa ctgaaagcaa tgaatgctca acctagtttc 660ctgcctcccg ttccttcaat ccctgctgca tttgcagctc aaggccaagc tggcggcaac 720aagttggttc cattcatcgg ctacccagga gttgctatgt ggcaattcat gccacctgct 780gcagttgata ccctcacaga tcatgtgctc c 811132833DNAGossypium raimondii 132cggagaatgg tgtcccctga aaactttaat tattggtctc acttcgatta tgctaccttg 60atccacgata tccctgtccc tgatgatcct tatgccggtt ttgcttggtc tacgcagcca 120atcgacgccc cttctaatgt tgtcagtgtg gaaattgatg gctcatttgg agattcagac 180ggtctaaagg aatctggttc aaagaagagg gttagatccg aatcatgcaa tgcatctagc 240tccaaagcat gtagggagaa gttgcgtaga gataggctaa atgacaagtt tatggagttg 300ggttctattt tggaacctgg aaggcctccc aaaactgata agtctgctat tttgattgat 360gctgtccgaa tggtgaccca gttacgaggt gaagcccaga aattgaagga ttcaaatact 420agtctacagg aaaggattaa agagttgaag tctgaaaaga atgagcttcg tgacgaaaag 480caaaggctga aggcagagaa agaaaggctg gagcagcaac tcaaagcaat gaatgcacaa 540cctagcttca tgccgcccgc accaccagca atccctgctg catttgctgc ggctccgggt 600caagctcctg ggaacaagtt ggtacctctc attggctatc ctggagttgc aatgtggcag 660ttcatgccgc ctgcagcagt ggatacttca caggaccatg tcctccgccc tccggttgcc 720taagttggca accaacaatg attggggttg cattttgatg caaacaaggg tataacgtaa 780tgttctgggc tgtttagatt tcgctgaagt ttttctaact ggctttgttg ggt 833133745DNACitrus reticulata 133aggtgaaact caaccgctgt ttgtttccca ggaaaatttc tctccgccgg aaaacccata 60aatattatcc tggaaattca aaatggtttc tccggaaaat acgaattggc tattagatta 120ccccttgatc gacgatatca ctgtccctga tggcaatttc tccgtttccg cttcgggttt 180cacttggacc gttcagccgc caattaacgg gccgtcaaat ggctgtgtgg aaattgattc 240tgcatttggg gattcaaatg gtctcaagga aagctcaaaa aagagggtta gatcagaatc 300atgtgggtct tctagctcca aggcgtgcag ggaaaagttg cgtagggatc gtcttaatga 360caagtttgtg gagttagcct ctatcttaga gcctggaagg ccccccaaaa cagataaggc 420agctattctg attgatgccg tccggatggt gactcaatta cgcagtgaag cccagaagtt 480gaaggactca aattcaagtc tccaggagaa gatcaaagag ttgaaggctg agaagaatga 540gcttcgtgat gagaagcaga ggctaaaggc agagaaagag aagatagagc agcaactcaa 600agctatgagc acacaaccca gtttcttaac tcctcctgca atccctgctg catttgctgc 660ccaaggccaa gcacctggaa acaagctgat gcctttcatc agctaccctg gagtcgcaat 720gtggcaattc atgcctcctg ctgca 745134916DNASolanum tuberosum 134gataatccaa acagcaattg gaaaccctca tcggagaatt ttccgggaga attatttttt

60tcatcttctg gccggcgatt gatcggagat ggtttcaccg gagaacacca actggcttta 120tgattatgga tttgaagata gttccgtccc tgattcgaat ttctcacctt ctgcatctgg 180gtttaactgg cctgtgcaga atttgaatgg ttcaaggaat gttagttctg aaattgatgg 240gtcgattggt gaatcagatt acccaaagga aagtggttct aagaaacggg caagggttga 300atcatgtgct ccaacaagtt ccaaagcttg cagagagaaa ctgcgaagag ataagctgaa 360tgacaagttc atggaattgg gtgcactcct tgagcctggc aggcccccca aaacagacaa 420atccgctatt cttgttgatg ctgttcgcat ggtgacccag ttacgtgatg aagctcaaaa 480gttgaaagac tcaaacttga atctgcaaga aaagatcaag gagttaaagg ttgagaaaac 540cgagcttcga gatgaaaaac agaggctgaa agctgaaaag gagaagctag agcaacaact 600aaagactaca agtgcgcaac ctagtttctt gcctcctgct ataccttctg catttgctgc 660tcatggtcaa tttccaggaa gcaagctggt gccaatcatg agttaccctg gtgtcgcgat 720gtggcaattc atgcctcctg ctgctgttga tacttcacag gaccatgtcc tccgtcctcc 780agttgcttaa cttgttgcag cttaaagcct acgaaggttg ccttcactgt cccgttaaat 840taatcgtcta gttaatgtcc ttcggttgta ttagttttgg ctcaactccc cttcctgtat 900ttggtggatg atagat 916135959DNASolanum tuberosum 135gatccctcat cggagaattt tccgggagaa ttattttttt catcttctgg ccggcgattg 60atccgagatg gtttcaccgg agaacaccaa ctggctttat gattatggat tcgaagatag 120ttccgtccct gattcgaatt tctcagcttc tgcatctggg tttaactggc ctgtgcagaa 180tttgaatggt tcaaggaatg ttagttctga aattgatggg tcgattggtg aatcagatta 240cccaaaggaa agtggttcta agaaacgggc aagggttgaa tcatgtgctc caacaagttc 300caaagcttgc agagagaaac tgcgaagaga taagctgaat gacaagttca tggaattggg 360tgcactcctt gagcctggca ggccccccaa aacagacaaa tccgctattc ttgttgatgc 420tgttcgcatg gtgacccagt tacgtgacga agctcaaaag ttgaaagact caaacttgaa 480tctgcaagaa aagatcaagg agttaaaggt tgagaaaacc gagcttcgag atgaaaaaca 540gaggttgaaa gctgaaaagg agaagctaga gcaacaacta aagactacaa gtgcgcaacc 600tagtttcttg cctcctgctg taccttctgc atttgctgct catggtcaat ttccaggaag 660caagctggtg ccaatcatga gttaccctgg tgtcgcgatg tggcaattca tgcctcctgc 720tgctgttgat acttcacagg accatgtcct ccgtcctcca gttgcttaac ttgttgcagc 780ttaatgccta caactgtccc attaaattaa tcgtctagtc aatgttcttc ggttgtatta 840gttttggctc aactcccctt actgtatttt ggtggatgat agataacttg tgactttgaa 900acttataacg gtttaatgct tgctttatgt gtaaaattaa ataaatttaa actataaaa 959136943DNASolanum tuberosum 136gtaattggaa accctcatcg gagaattttc cgggagaatt atttttttca tcttctggcc 60ggcgattgat cggagatggt ttcaccggag aacaccaact ggctttatga ttatggattc 120gaagatagtt ccgtccctga ttcgaatttc tcagcttctg catctgggtt taactggcct 180gtgcagaatt tgaatggttc aaggaatgtt agttctgaaa ttgatgggtc gattggtgaa 240tcagattacc caaaggaaag tggttctaag aaacgggcaa gggttgaatc atgtgctcca 300acaagttcca aagcttgcag agagaaattg cgaagagata agctgaatga caagttcatg 360gaattgggtg cactccttga gcctggcagg ccccccaaaa cagacaaatc tgctattctt 420gttgatgctg ttcgcatggt gacccagtta cgtgatgaag ctcaaaagtt gaaagactca 480aacttgaatc tgcaagaaaa gatcaaggag ttaaaggttg agaaaaccga gcttcgagat 540gaaaaacaga ggctgaaagc tgaaaaggag aagctagagc aacaactaaa gactacaagt 600gcgcaaccta gtttcttgcc tcctgctata ccttctgcat ttgctgctca tggtcaattt 660ccaggaagca agctggtgcc aatcatgagt taccctggtg tcgcgatgtg gcaattcatg 720cctcctgctg ctgttgatac ttcacaggac catgtcctcc gtcctccagt tgcttaactt 780gttgcagctt aaagcctaca aaggttgcct tcactgtccc gttaaattaa tcgtctagtc 840aatgtccttc ggttgtatta gttttggctc aactcccctt actgtatttg gtggatgata 900gataacttgt gactttgaaa cttataacgg tttaatgctt gct 943137917DNAVitis vinifera 137tttttttttt tttttttttt tttttttttt ccagattaaa cttattgaga tttacatgca 60aataccatca ctaattcagg taagttctta aattgcaaac atcttccatc acccacgaaa 120tccagttaga accaggaaca ctaatagtct aatacagtcc agaaacgaca aaatgaaagt 180aaacttcttt ggggcaaaat gcaaacccaa atcattagag gttgagttgc tgatttaagc 240aactggggga cggagcacat gatcttgtga ggtatcaact gcagcagggg gcatgaattg 300ccacatagca actcctgggt agccgatgaa tggaaccaac ttgttgccgc cagcttggcc 360ttgagctgca aatgcagcag ggattgaagg aacgggaggc aggaaactag gttgagcatt 420cattgctttc agttgctgct ccagtttctc tttctcggcc tttagccttt gcttctcatc 480acgaagctca ttcttctcag ccttcaattc tttaatcttc tcctggagac tagaatttga 540gtccttcaac ttctgcgact cacctcgtaa ctgagttacc attcgaactg catcaatcaa 600aatagaagac ttgtctgttt ttggaggcct tccaggctcc aggatagaac ccaattccat 660aaacttgtca tttagcctgt ccctccgcaa cttctcccta catgccttcg agctagatgc 720accacatgat tcagatctca ccctcttctt tgatccagtt tccttaatgc cgtcagagtc 780cccaaatgaa ccatcaattt ccccactgac attagcagaa gtgttcaagg cctgcacagg 840agtccaggcg aaccctgaat tcgtattagc gaagtttgaa tcagggacag ggatgtcctc 900gatgagcccg tactcgt 917138652DNAGlycine max 138tccaaagcgg aaaacagcaa ttggctgttt gattacccgt tgatcgacga cgacgttatt 60cccgtcggcg actcctcctt cgccgtctcc gcttccacct tctcctggcc cccacctccc 120gccaatgtca gtgtcgaaat tgatgcttcg cttggggatt ctgatggcct aaaaaatcct 180gctttgaaga aaaggactaa atctgattca agtactgctt ctagctccaa agcgtgtcgg 240gagaagttga ggagggatag gcttaatgac aagtttgttg aattgggctc catcttggag 300cccggaaggc ctcccaaaac agacaaggct tccattctga ttgatgctgc ccgaatggtg 360acacagctgc gggatgaagc cctgaagttg aaagactcaa atacgagtct tcaagagaag 420attaaagagt taaaggctga gaagaatgaa cttcgtgatg agaaacagag gcttaaggca 480gagaaagaga agttggaggt gcaggtaaaa tcaatgaatg ctcaacctgc tttcttgcca 540ccccctcctg caatccctgc tgcatttgct ccacaaggcc aagcccctgg caacaagttg 600gtgcctttca tcagctatcc gggagttgcc atgtggcaat ttatgcctcc gg 652139955DNASolanum tuberosum 139gatcaattgg aaaccctcat cggagaattt tccgggagaa ttattttttt catattctgg 60ccggcgattg atcggagatg gtttcaccgg agaacaccaa ctggctttat gattatggat 120ttgaagatag ttccgtccct gattcgaatt tctcagcttc tgcatctggg tttaactggc 180ctgtgcagaa tttgaatggt tcaaggaatg ttagttctga aattgatggg tcgattggtg 240aatcagattg cccaaaggaa agtggttcta agaaacgggc aagggttgaa tcatgtgctc 300caacaagttc caaagcttgc agagagaaac tgcgaagaga taagctgaat gacaagttca 360tggaattggg tgcactcctt gagcctggca ggccccccaa aacagacaaa tccgctattc 420ttgttgatgc tgttcgcatg gtgacccagt tacgtgatga agctcaaaag ttgaaagact 480caaatttgaa tctgcaagaa aagatcaagg agttaaaggt tgagaaaacc gagcttcgag 540atgaaaaaca gaggctgaaa gctgaaaagg agaagctaga gcaacaacta aagactacaa 600gtgcgcaacc tagtttcttg cctcctgcta taccttctgc atttgctgct catggtcaat 660ttccaggaag caagctggtg ccaatcatga gttaccctgg tgtcgcgatg tggcaattca 720tgcctcctgc tgctgttgat acttcacagg accatgtcct ccgtcctcca gttgcttaac 780ttgttgcagc ttaaagccta caaaggttgc cttcactgtc ccgttaaatt aatcgtctag 840tcaatgtcct tcggttgtat tagatttggc tcaactcccc ttactgtatt tggtggatga 900tagataactt gtgactttga aacttataac ggttttatgc ttgctttatg tgtaa 955140792DNACitrus reticulata 140ccttttttgc cgacgaactt attgaaattt acatgcaaaa gccagcaaca atccactatt 60gacagagaga ttcaaataca gtcacccacc aatcattaaa gacttcggaa aaactaatac 120agtcagagaa caatgataaa acgaatacac ttgttgggtg caaattgcaa acccaaatca 180ttgtaggttg catacttaag caaccggagg acggagtaca tgatcctgtg aggtatccac 240tgcagcagga ggcatgaatt gccacattgc gactccaggg tagctgatga aaggcatcag 300cttgtttcca ggtgcttggc cttgggcagc aaatgcagca gggattgcag gaggagttaa 360gaaactgggt tgtgtgctca tagctttgag ttgctgctct atcttctctt tctctgcctt 420tagcctctgc ttctcatcac gaagctcatt cttctcagcc ttcaactctt tgatcttctc 480ctggagactt gaatttgagt ccttcaactt ctgggcttca ctgcgtaatt gagtcaccat 540ccggacggca tcaatcagaa tagctgcctt atctgttttg gggggccttc caggctctaa 600gatagaggct aactccacaa acttgtcatt aagacgatcc ctacgcaact tttccctgca 660cgccttggag ctagaagacc cacatgattc tgatctaacc ctcttttttg agctttcctt 720gagaccattt gaatccccaa atgcagaatc aatttccaca cagccatttg acggcccgtt 780aattggcggc tg 792141840DNAThellungiella halophila 141ggcacgaggc ctcgtgccga attcggcacg aggccgaaaa cgctaattgg atttgcgacc 60tgattgatgc cgactatgga agtttcacaa tccaaggtcc aggtttctct tggcccgtgc 120agcaacctat tggcgtttct tcaaactcca gcgcgggagt tgatgtctca gctggaaatt 180cagaagccag caaggaacct ggctccaaaa agaggggtag atgtgaatca tcctctgcca 240ctggctcaaa agcatgtaga gagaagctgc gacgtgacag attgaatgac aagtttacgg 300aattgggtgc aattttggag cctgggaatc ctcccaaaac agacaaggct gcaatcttgg 360ttgatgctgt ccgcatggtg gcacagctac ggggcgaagc ccagaagttg aaggactcca 420attcaagtct ccaggacaaa atcaaagagt taaagactga gaaaaacgag ttgcgagatg 480agaaacagag gctgaagaca gagaaagaga agctggagca acagctgaaa accatgaatg 540ctcctcaacc aagctttttc ccagctccac ctatgatgcc aactgctttt gcttctgcac 600aaggccaagc tcccggaaac aagatggtgc caatcatcag ttacccagga gttgccatgt 660ggcagttcat gcctcctgct tcagtcgata cttctcagga tcatgtcctt cgtccaccag 720ttgcttaact gggagacaaa gacctcagga aaaaaaatca tcaattggtt tggcttctcg 780cttacgctga aaggaaaggc tccatttgtt ttgcctctct ctttttcggc tctcttagcc 840142822DNAPopulus tremuloidesmisc_feature(788)..(788)n is a, c, g, or t 142cggcggctca ccggaacaca cgccgggaac cttgaattcc ggcggagatg gtgttaccta 60atgaaaatga taactgggtt tttgattgtg ggttgattga ggacatttcg gtccctggtg 120gtgaccttct tggtcttgaa tctcttgatg aaaccccgaa tgggtctctg tggtcttctc 180ataatttcac tgattctgcc ttcttaagtg tggaattcaa taattcatat gagaattcgg 240atggccataa ggaaagtggg tgtcggaaac gagtgaggcc tggatcaagt aatgcaactg 300gctccaaagc atgtagagag aaactgcggc gggataggct gaacgacagg ttcatggaat 360tgggtgctct tctagatcct ggaaggcctc ctaaagtgga caaatctgct atactggttg 420atgctgctcg aatggtgact cagttacgag atgaatctca gaagctgaaa gagtctaatg 480tgagtctaca ggagaagatc gatgaattga aggcggagaa gaatgagctt cgtgatgaga 540aacagaggct aaagacagaa aaggagaacc tagagcggca agtgaaagcc ttgagtgctc 600caccaaactt cctgcctcat ccctctgcca ttccagctcc attttctgcc ccaggccaag 660ttgttggcag caagatgatg ccctttgttg gttatcctgg aatttctatg tggcagttca 720tgccccctgc tgttgttgat acctctcagg atcatgttct acgccctcca gttgcttaag 780ttattcangc agaaatttta tgtctacgtc ctcgcagagc at 822143911DNAVitis vinifera 143atgtgaacgg tggtgtgtga tggtgaatgg tgccaccgat ccctaaaacc agaaaaaaaa 60agaagaaaaa aacccaaatt catttctctt ctcaccaccg actttaattt cgccggagaa 120aactccggta tggtatctcc agaagaagat ccaaattgga tcttcgacta cggcttgatc 180gacgacgttc ctgtcccctc cctccaagca acctttaatt ggccttctca tgatttcact 240gcttccgtcg ccctcggtgt ggaatttgat gactcacctg tgaatttgga tgatgtgaag 300gaaaatcact cccggaaaag gatgaggtct ggactgtgca gcgcgtctgg ctccaaagca 360tgtcgggaga aagtacggag ggataggctg aatgacaggt tcctagaatt gggttctatc 420ctggagcctg gaagaccccc taaaatggac aaggctgtta tattaagtga tgctcttaga 480atgatgactc agctgcgtag tgaaggacag aagctgaaga aatcatgtga ggatctgcaa 540gagaagatca atgaattgaa ggctgagaag aatgagcttc gtgatgagaa gcagaggctg 600aagacagaga aagagaacat tgtgcagcaa ataaaagctc tgagtaccca agcaggcttc 660ctgccacacc cttctgcaat cccagctcca tttgccgctc caggccaagt tgttggcagc 720aagctgatgc ctttcattgg ctaccctgga gtttccatgt ggcagttcat gccacctgct 780gccgttgata cttcacagga tcatgttctc cggcccccag ttgcttaaat ttgaaggcgt 840taactgtgga tcttccttcc ctgtggatcg agcatgattc tatgatctgg gttttcttct 900ggctcttgaa g 911144849DNABrassica napus 144tgttataaga agataacacc ggagaagacc aaagcaacca taagactaag aaagccgaga 60aagaggagag caaaacaaat ggacttttct tgcttttttt ctttttttcc ggrgragcaa 120gaagcaaacc agttgatgat tttcttgagg gtcgtcccag ttaagcaact ggtggacgaa 180ggacgtgatc ctgagaagta tcgactgaag caggaggcat gaactgccac atggcaactc 240ctgggtaact gatgactggc accatcttgt ttccgggagc ttggccttgc gcagaagcaa 300aagcagttgg catcataggt ggggctggga agaagcttgg ttgaggagga gcattcatgg 360ctttcagctg ttgctccagc ttctctttct ctgtcttcag cctctgcttc tcatctcgca 420gctcgttctt ctcggtcttt aactctttga ttttgtcttg aagactcgaa ttggagtcct 480tcaacttctg cgcctcgcca cgtagttgtg taaccatgcg gacagcatcg accaagattg 540cagccttgtc tgtsttggga ggattccccg gctccaaaat tgcacccaat tccataaact 600tgtcattcag cctgtcacgt cgctgcttct ctctacaggc tttagagcta gtggcagagg 660acgattcaca tctcgccctc tttttggagc aggttccttg gtggcttctg aatttccagc 720tgagcatcac tchagactgg aacgccatag gtgctggtga gaggcaggag agcaggcttg 780aatgtgaact tcatagtctg cgtygatcaw tcggtatcaa tagcgtttcg gggcacatct 840ctcaactca 849145852DNAVitis vinifera 145tttttttttt tttttttttt tttttttttc gtaatgtaag ttgccagatt aaacttattg 60agatttacat gcaaatacca tcactaattc aggtaagttc ttaaattgca aacatcttcc 120atcacccacg aaatccagtt agaaccagga acactaatag tctaatacag tccagaaacg 180acaaaatgaa agtaaacttc tttggggcaa aatgcaaacc caaatcatta gaggttgagt 240tgctgattta agcaactggg ggacggagca catgatcttg tgaggtatca actgcagcag 300ggggcatgaa ttgccacata gcaactcctg ggtagccgat gaatggaacc aacttgttgc 360cgccagcttg gccttgagct gcaaatgcag cagggattga aggaacggga ggcaggaaac 420taggttgagc attcattgct ttcagttgct gctccagttt ctctttctcg gcctttagcc 480tttgcttctc atcacgaagc tcattcttct cagccttcaa ttctttaatc ttctcctgga 540gactagaatt tgagtccttc aacttctgcg actcacctcg taactgagtt accattcgaa 600ctgcatcaat caaaatagaa gacttgtctg tttttggagg ccttccaggc tccaggatag 660aacccaattc catagacttg tcatttagcc tgtccctccg caacttctcc ctacatgcct 720tcgagctaga tgcaccacat gattcagatc tcaccctctt ctttgatcca gtttccttaa 780tgccgtcaga gtccccaaat gaaccatcaa tttccccact gacattagca gaagtgttca 840aggcctgcac ag 852146837DNAGossypium raimondiimisc_feature(831)..(831)n is a, c, g, or t 146caccaatcct ctgtgtcccg ccctttgttc ttatttttct ctcttgccaa acagaaactc 60cattttcttt gccaaaagcc ggaaatcaaa ccaaaccctt ccggctaaaa gcaaattcag 120tggagaatgg tatcacctga aaacaccaat tattggtcta gcttcgatta tgcaaccttg 180atcaacgata tccctgcccc tgacggacct tattccggat tttcttggcc cactcggcca 240atcaatgcat cttctaatgt tttcagtgtg gaaactgacg gctcgtttga ggattcagat 300ggccttaagg aatctggttc aaagaagaga gttagatctg agtcttgcaa tgtttcaagc 360tccaaagcat gcagggagaa gttgcgtagg gataagctaa atgagaagtt tatggagctg 420agttctattt tggaacctga aaagcctccc aagacagaca aggctgctat tttggttgat 480gctgtccgaa tggtaaccca gttacgaggt gaagcccaga aattgaagga ttcaatttca 540agtctccatg acaggattaa agaattgaag gctgaaaaga atgaacttcg tgatgaaaag 600caaaggctga aggccgagaa ggaaaagctg gagcaacagc tgaaggccat gaattcacaa 660cccagcttca tgcctcctgc acctgcattc cctgctgcat ttgctactgc ccaaggtcaa 720gttccaggaa acaagttggt tcctttcttt ggttatcctg gagttgccat gtggcagttt 780atgctgcctg cgtcgttaga cacctcagag gatcatgtac tccgccctcc ngttgcc 837147806DNAPopulus tremuloides 147cggcggctca ccggaacaca cgccgggaac cttgaattcc ggcggagatg gtgttaccta 60atgaaaatga taactgggtt tttgattgtg ggttgattga ggacatttcg gtccctggtg 120gtgaccttct tggtcttgaa tctcttgatg aaaccccgaa tgggtctctg tggtcttctc 180ataatttcac tgattctgcc ttcttaagtg tggaattcaa taattcatat gagaattcgg 240atggccataa ggaaagtggg tgtcggaaac gagtgaggcc tggatcaagt aatgcaactg 300gctccaaagc atgtagagag aaactgcggc gggataggct gaacgacagg ttcatggaat 360tgggtgctct tctagatcct ggaaggcctc ctaaagtgga caaatctgct atactggttg 420atgctgctcg aatggtgact cagttacgag atgaatctca gaagctgaaa gagtctaatg 480tgagtctaca ggagaagatc gatgaattga aggcggagaa gaatgagctt cgtgatgaga 540aacagaggct aaagacagaa aaggagaacc tagagcggca agtgaaagcc ttgagtgctc 600caccaaactt cctgcctcat ccctctgcca ttccagctcc attttctgcc ccaggccaag 660ttgttggcag caagatgatg ccctttgttg gttatcctgg aatttctatg tggcagttca 720tgccccctgc tgttgttgat acctctcagg atcatgttct acgcccctca gttgcttaag 780ttattcaggc agggatttta tgtcta 806148598DNAPrunus dulcis 148caaaaaagag ggtcagaact gaatcatgca gtggatctag ttccaaggca tgcagggaga 60agttgcgaag ggataggcta aacgacaagt ttctcgaatt gggctctata ttggagcctg 120gaaggccccc caagactgac aaggctgcta tattggtgga tgctgtccga atggtgaatc 180agttacgcgg tgaagcccaa aagttgaagg actcaaattc aagcctccag gagaagatca 240aggaattgaa ggcagagaag aatgaacttc gtgatgagaa gcagaggttg aagttagaga 300aagagaagtt ggagcagcaa ctgaaggcca tgaatgcaca gccaggcttc ttgcctcccc 360ctcctgcaat tcctgctgca tttgctgccc aaggccaagc tcacggcaac aagctggtgc 420ctttcattgg ataccctggg gttgccatgt ggcagttcat gccacctgcc tcagtggata 480cttcgcagga tcatgtactc cgcccaccag ttgcttaagt tggcaagatc taagatgatt 540cggctgattt gggataacat tttgtcccca acatgtttat tgtcctgtca ttgctctt 598149568DNAGlycine max 149cctgctttga agaaaaggac taaatctgat tcaagtactg cttctagctc caaagcgtgt 60cgggagaagt tgaggaggga taggcttaat gacaagtttg ttgaattggg ctccatcttg 120gagcccggaa ggcctcccaa aacagacaag gcttccattc tgattgatgc tgcccgaatg 180gtgacacagc tgcgggatga agccctgaag ttgaaagact caaatacgag tcttcaagag 240aagattaaag agttaaaggc tgagaagaat gaacttcgtg atgagaaaca gaggcttaag 300gcagagaaag agaagttgga ggtgcaggta aaatcaatga atgctcaacc tgctttcttg 360ccaccccctc ctgcaatccc tgctgcattt gctccacaag gccaagcccc tggcaacaag 420ttggtgcctt tcatcagcta tccgggagtt gccatgtggc aatttatgcc tccggccgcc 480gtggatacct cacaggatca tgtactccgt ccaccggttg cctaagttgg cattgtacaa 540ttatttgggc ttccattttg gctcaaaa 568150708DNAMedicago truncatula 150atagaaaact aacaaagcaa gaaggtattg gtgataggat acaatcttga gctaaaatgg 60aagcccggag aattgcacag agccagctta ggcaactgga ggacgaagta catgatcctg 120tgaggtatcc actgcagcag gtggcataaa ttgccacatt gcaactccag gataactaat 180aaagggtacc aatttgttgc catgggcttg gccttgtgca gcaaatgccg cagggagtgc 240agtgggggta ggcaagaaac taggaggtgc attcatagat ttcagctgct gctccaactt 300ctctttctct gccttgagcc tctgtttctc gtcacggagt tcgttcttct caacctttaa 360ctctttaatc ttttcttgaa gacccgagtt ggcatctttc aacttttggg cttcacccct 420taactgtgtc accattcgga cagcatcaat caggatggca gccttgtctg ttttggcagg 480ccttccaggc tccaaaatgg agcccaattc aataaacttg tcgttaagcc tatctctacg 540caacttctcc cgacatgctt tggagctagt agcagcacat gactcagatc taaccctctt 600ctttgaacca gactctttga gaccgtcaga atcccctatg agcccatcac ttcagcactg 660acattggaaa aaaggttgaa ggggggctga attggccagg tgaaagcg 708151797DNAMedicago truncatula 151ttaattcata agtattcata agccaaatac aaacggaagc cactgggtcc agttagaata 60gatagaaaac taacaaagca agaaggtatt ggtgatagga tacaatcttg agctaaaatg 120gaagcccgga gaattgcaca gagccagctt aggcaactgg aggacgaagt acatgatcct 180gtgaggtatc cactgcagca ggtggcataa

attgccacat tgcaactcca ggataactaa 240taaagggtac caatttgttg ccatgggctt ggccttgtgc agcaaatgcc gcagggagtg 300cagtgggggt aggcaagaaa ctaggaggtg cattcataga tttcagctgc tgctccaact 360tctctttctc tgccttgagc ctctgtttct cgtcacggag ttcgttcttc tcaaccttta 420actctttaat cttttcttga agacccgagt tggcatcttt caacttttgg gcttcacccc 480ttaactgtgt caccattcgg acagcatcaa tcaggatggc agccttgtct gttttggcag 540gccctccagg ctccaaaatg gagcccaatt caataaactt gtcgttaagc ctatctctac 600gcaacttctc ccgacatgct ttggagctag tagcagcaca tgactcagat ctaaccctct 660tctttgaacc agactctttg agaccgtcag aatcccctaa tgagccatca acttcagcac 720ccattctcat gtcaacagca tcagtccatg gaaagttgca ctgtttgaag ttagacagcg 780tacccctatc tcactaa 797152524DNAGlycine max 152agggctagat gtgattcaag tactgcttct agctctaaag cgtgtcggga gaagttgcgg 60agggataggc ttaatgacaa atttgttgaa ttgggctcca tcttggagcc tggaaggcct 120cccaaaacag acaaggctgc cattctgatt gatgctgccc gaatggtaac acagctgcgg 180gatgaagccc tgaagttgaa agactcaaat acgagtcttc aagagaagat taaagagtta 240aaggctgaga agaatgaact tcgtgacgag aaacagaggc ttaaggcaga gaaagagaag 300ttggagatgc aggtaaaatc aatgaatgct caacctgctt tcttgccacc ccctcctgca 360atccctgctg catttgctcc acaaggccaa gcccccggca acaagttgat gcctttcatc 420aggtacccgg gagttgccat gtggcaattt atgcctccgg ccaccatgga tacctcccag 480gatcatgttc tccgtccacc agttgcctaa gttggcattg taca 524153738DNAVitis vinifera 153cgcaagtgtt tgttccaaag atgtacttgg cttacttcaa cagttgattt aaaaacatac 60aacaatcata tttgcttatt gtattttaca tgcaatcagc agaaatccaa gggagtcatt 120ggtttctgat aacaatgaca aagtaaaata gacgaggaac tctttgggca ctgagaatgt 180agaccaacca ttgatgtcct ccaacttaag ccactggggg acggagaaca tgatcctgtg 240atgtgtcaac tgcagcaggt ggcatgaatt gccacatggc aacactaggg taaccaatga 300aaggcatcag cttgttgcca ggagctcggc cttgggcagc aaatgcagct ggcattgcag 360aaggatgagg cagaaagcct ggctgtgcac taatggcttt gacttgctgc tccagcttct 420ccttttcggc ttttaacctt tgcttctcat cccgaagctc atttttctca gccttcaact 480ccttaatctt ctcttgcaaa tccccatttg actccttcag cttctgtgct tcacttcgta 540attgagtcac cattcgaaca gcatcactca gaatagcagc cttgtccgtt ttgggtggcc 600ttccaggctc caagatagaa cccaattcca agaacctctc attcagccta tccctccgca 660atttttcccg gcaagctttg gtgccagttg caccacatga gattcatgct tcaaccgttt 720tcggggtcca agttcttt 738154559DNAGlycine soja 154tgatggctcg ttgggggatt ctgacagtct caaagagtct ggctcaaaga aaagggttag 60gtctgagtca tgtgctgctt ctggctccaa ggcatgtcgg gagaagttga gaagggatag 120gcttaatgac aagtttgttg aattgggcgc cattttggag cctggaaggc ctgccaaaac 180agacaaggct gctatcctga ttgatgctgt ccgaatggtg acccagttac ggggtgaagc 240ccagaagttg aaagacacta atcagggtct tcaagaaaag attaaagagt taaaggctga 300gaagaacgaa cttcgcgatg agaaacagag gctcaaggca gagaaggaga aactggagca 360gcagctgaaa tctttgaatg cacagcctag tttcatgcct ccccctgctg caatgcctgc 420tgcatttgcg gcacaaggcc aagcccatgg caacaagttg gtacctttta ttagttattc 480cggaagttgc atgtggcaat tcatgccacc tgctgcagtg gatacctcac aggatcatgt 540tctccgtcca ccagtttcc 559155943DNASolanum tuberosum 155gtgggaatcc taattggtta tttgattatg agttgataac ggatattact tctgctgctt 60ctgttaccgt cactgatttt cagtctccgg ctactattga tttcagctgg cctgctcaaa 120caatctatgc ttcctctaat ctcattgctg aaacagatta cacatttgcg gattcagaag 180tcagtaagga ggcaagctca cgaaagaggt taaaaagtga atggtgcagc tctccgagat 240ctaaggcatg ccgagagaaa ttgcggaggg acagactgaa tgagaggttc ctcgaattga 300gctctgtcct tgatcctgga aggccaccaa aaactgagaa agttgcaatt ctgagtgatg 360ctcaaaggat gctgattgag ctgcgaactg aaacccagaa gctgaaggag tcaaatgagg 420agctgcaaga gaagataaaa gaacttaagg cagagaagaa tgagctccgt gatgaaaagc 480gaaggctaaa ggaagaaaag gagaatttgg agcagcaggt taaaagctta gcttctaaac 540caggatttct ctcccatcct tctgccgtgg gagctgcatt tactgcacaa ggacaagtcg 600ctgcaggcaa caaattgatg cctttcattg gttatcccag tgttgcaatg tggcaattca 660tgcaacctgc tgttgttgac acatctcaag atcatgtgct ccgtcctcca gttgcttaaa 720ctgaatttgc caggtgactt gctactggaa ctttgttctt tttgttgctc ctgtgagtgc 780taaaggtgac aaacaactga gacaatttgc tggttgtatt tatatgtcta gcttttatgt 840atgagtcagt cttgatgatt tgctaaatca tgtatatgac gggtatatac tcacccgaaa 900aacagttacg tattatgatt ccctagatgg gactccttct gaa 943156831DNAGossypium raimondii 156ctcccaccaa tcctctgtgt cccgccctct ttttttagtt ttctctcttg ccaaacagaa 60actccattct tttttgccaa aagccggaaa tcaaaccaaa cccttccggc taaaagcaaa 120ttcagtggag aatggtatca cctgaaaaca ccaattattg gtctagcttc gattatgcaa 180ccttgatcaa cgatatccct gcccctgacg gaccttattc cggattttct tggcccactc 240ggccaatcaa tgcatcttct aatgttttca gtgtggaaat tgacggctcg tttgaggatt 300cagatggcct taaggaatct ggttcaaaga agagagttag atctgagtct tgcaatgttt 360caagctccaa agcatgcagg gagaagttgc gtagggataa gctaaatgag aagtttatgg 420agctgagttc tattttggaa cctgaaaagc ctcccaagac agacaaggct gctattttgg 480ttgatgctgt ccgaatggta acccagttac gaggtgaagc ccagaaattg aaggattcaa 540tttcaagtct ccatgacagg attaaagaat tgaaggctga aaagaatgaa cttcgtgatg 600aaaagcaaag gctgaaggcc gagaaggaaa agctggagca acagctgaag gccatgaatt 660cacaacccag cttcatgcct cctgcacctg cattccctgc tgcatttgct actgcccaag 720gtcaagttcc aggaaacaag ttggttcctt tctttggtta tcctggagtt gccatgtggc 780agtttatgct gcctgcgtcg ttagacacct cagaggatca tgtactccgc c 831157791DNAVitis viniferamisc_feature(725)..(725)n is a, c, g, or t 157catttttccc actcggattc ccttttctcc ctcgagaaaa cccccctttt ctctctcccc 60aaacacccta acgcgctcct ccctctcccg ggaaattgca ccgggcctcc tcgatttttc 120ggaggttcct tcgagatggt ttctccagaa gccaccaatt ggctgtacga gtacgggctc 180atcgaggaca tccctgtccc tgattcaaac ttcgctaata cgaattcagg gttcgcctgg 240actcctgtgc aggccttgaa cacttctgct aatgtcagtg gggaaattga tggttcattt 300ggggactctg acggcattaa ggaaactgga tcaaagaaga gggtgagatc tgaatcatgt 360ggtgcatcta gctcgaaggc atgtagggag aagttgcgga gggacaggct aaatgacaag 420tctatggaat tgggttctat cctggagcct ggaaggcctc caaaaacaga caagtcttct 480attttgattg atgcagttcg aatggtaact cagttacgag gtgagtcgca gaagttgaag 540gactcaaatt ctagtctcca ggagaagatt aaagaattga aggctgagaa gaatgagctt 600cgtgatgaga agcaaaggct aaaggccgag aaagagaaac tggagcagca actgaaagca 660atgaatgctc aacctagttt cctgcctccc gttccttcaa tccctgctgc atttgcagct 720caagnccaag ctggcggcaa caagttggtt ccattcatcg gctacccagg agttgctatg 780tggcaattca t 791158693DNALycopersicon esculentum 158catcttctgg ccggcgactg atcggagatg gtttcaccgg agagtaccaa ttggctttat 60gattacggat tcgaagatag ttgcgtccct gattcgaatt tctcagcttc tgcatctggg 120tttaactggt ctgtgcagaa tttgaatggt tcaaggaatg ttagttctga aatcgatggg 180tcaattggtg aatcagatta ccccaaggaa agtggttcta agaaacgggc aagggttgaa 240tcatgtgctc caacaagttc caaagcttgc agagagaaac tgcgaagaga taggctgaat 300gacaagttca tggaattggg tgcactcctt gagcctggaa gaccccctaa aacagacaaa 360tccgctattc ttgttgatgc tgttcgcttg gtgacccagt tacgtgatga agctcaaaag 420ttgaaagact caaacttgaa tctgcaagaa aagatcaagg agttaaaggt tgagaaaacc 480gagcttcgag atgaaaaaca caggctgaaa gctgaaaagg agaagctaga gcaacaacta 540aagactacaa gtgcacggcc tagttacttg cctcctgcta taccttctgc atttgctgct 600catggtcaat ttccaggaag caagctggtg ccaatcatga gttacccctg tgtcccgatg 660tggcaattca tgcctcctgc tgctgttgat act 693159877DNAPinus taeda 159ctggacacct cgaatctcct ccggtcattt tttgttttga cagggccggt ttggtgatta 60gggtttcaga taacaggagg acaagtgttc gaattcgaga gaagccagaa tgagctctcc 120gcagagcaat aagtggctgt catatttcga cgagccattg ttggatgatg taggcgtggg 180gcagccggcc aatccattct tctggtgcgg tcagggcata aatgatcagc ccgacgtaag 240tggtagtgta gaaattgatg gccccaataa ggacatggac gagcaagata aattatgtcc 300tagaaagagg tcacgggaag aatctagtgg gggacctggg tcaaaagctt gccgtgagaa 360gatgcggagg gacagactta atgataggtt catggagcta agctctgtgt tagaaccggg 420taggcctccc aagacggcag acaaagccac aattttgtct gatgctgcac gtgttatgac 480ccagctacga actgaggcgc agaacctgaa agctgagaat gaacgactgc aggaagccat 540taaagatctg aaggcagaga aaaatgaact tcgtgatgaa aagctgagaa tgaaagcaga 600aaaggaaaaa ttggagcaac aagtaaaagc aatggctttg cccacaggct ttgtgccgca 660tcctgcagca tttcatgcgg ctgctgcttt tgcagcccaa agtcaagcag cagcaaacaa 720aactatgcct gttccaggat atcctggaat ggcaatgtgg caatggatgc ctccagctgt 780ggttgatact tcgcaggatc atgtgctaag gcctcctgtt gcttgaagca ggctcttatt 840ttatattcca aactggtgct actatttctt tggccct 87716025PRTArtificialplant consensus 160Xaa Xaa Cys Xaa Val Cys Xaa Xaa Xaa Xaa Xaa Xaa Tyr Gln Ala Leu1 5 10 15Gly Gly His Lys Xaa Ser His Arg Xaa 20 2516125PRTArtificialdicotyledonous consensus 161Xaa Xaa Cys Xaa Val Cys Xaa Lys Xaa Phe Xaa Ser Tyr Gln Ala Leu1 5 10 15Gly Gly His Lys Xaa Ser His Arg Xaa 20 2516225PRTArtificialmonocotyledonous consensus 162Xaa Xaa Cys Ser Val Cys Gly Xaa Xaa Xaa Xaa Ser Tyr Gln Ala Leu1 5 10 15Gly Gly His Lys Xaa Ser His Arg Xaa 20 25163228PRTLolium perenne 163Met Ala Val Glu Ala Val Leu Glu Ala Ala Ala Met Ile Gln Ser Pro1 5 10 15Pro Ser Lys Lys Met Glu Ala Ser Ser Ser Ser Asp Glu Ala Phe Glu 20 25 30Ala Leu Gln Gln His Thr Glu Gly Trp Ser Lys Lys Lys Arg Ser Arg 35 40 45Arg Pro Arg Ala Leu Glu Pro Ser Glu Glu Glu Tyr Leu Ala Phe Cys 50 55 60Leu Val Met Leu Ala Arg Gly His Arg Asp Ala Ala Pro Glu His Gly65 70 75 80Cys Ser Val Cys Gly Lys Ala Phe Ala Ser Tyr Gln Ala Leu Gly Gly 85 90 95His Lys Ala Ser His Arg Lys Pro Pro Thr Ala Pro Ala Ala Val Ala 100 105 110Ala Ser Ala Val Pro Glu Glu Asp Lys Pro Arg Ala Ala Ala Ser Ser 115 120 125Ser Ser Gly Ser Gly Asp Ala Ala Gly Gly Gly Lys Val His Glu Cys 130 135 140Asn Val Cys Gln Lys Thr Phe Pro Thr Gly Gln Ala Leu Gly Gly His145 150 155 160Lys Arg Cys His Tyr Asp Gly Thr Ile Gly Ser Ala Ala Ala Pro Thr 165 170 175Val Lys Ala Ala Lys Ala Ala Ala Ala Ala Ser Ala Pro Thr Ala Thr 180 185 190Asn Arg Gly Phe Asp Leu Asn Val Pro Ala Leu Pro Gly Leu Ala Glu 195 200 205Glu Gly Glu Glu Val Leu Ser Pro Val Ser Phe Lys Lys Pro Arg Leu 210 215 220Met Ile Thr Ala225164250PRTOryza sativa 164Met Ala Val Glu Ala Val Leu Glu Ala Ser Arg Ser Ser Ser Glu Glu1 5 10 15Glu Ala Glu Val Ile Val Thr His Gly Gly Gly Gly Gly Gly Gly Gly 20 25 30Gly Gly Gly Gln Val Glu Gly Trp Gly Lys Arg Lys Arg Ser Arg Arg 35 40 45Arg Arg Pro Gln Leu Pro Pro Ser Glu Glu Glu Tyr Leu Ala Leu Cys 50 55 60Leu Leu Met Leu Ala Arg Gly Arg Arg Asp Gly Asp Asp Val Ala Ala65 70 75 80Ser Ala Ser Ala Ala Ala Ala Ala Val Glu His Arg Cys Ser Val Cys 85 90 95Gly Lys Ala Phe Ala Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser 100 105 110His Arg Lys Pro Pro Pro Pro Pro Pro Pro Ala Met Val Asp Asp Asp 115 120 125Glu Val Val Val Glu Thr Lys Pro Ala Ala Ile Ala Thr Pro Ser Ser 130 135 140Ser Ala Ser Gly Val Ser Gly Gly Gly Gly Gly Arg Ala His Glu Cys145 150 155 160Asn Val Cys Gly Lys Ala Phe Pro Thr Gly Gln Ala Leu Gly Gly His 165 170 175Lys Arg Cys His Tyr Asp Gly Thr Ile Gly Ser Ala Ala Gly Ala Gly 180 185 190Ala Ser Lys Pro Ala Ala Lys Thr Thr Val Ala Val Ala Ala Ser Arg 195 200 205Gly Phe Asp Leu Asn Leu Pro Ala Leu Pro Asp Val Ala Ala Ala Ala 210 215 220Asp Gln Arg Cys Ala Ala Glu Asp Asp Glu Val Leu Ser Pro Leu Ala225 230 235 240Phe Lys Lys Pro Arg Leu Met Ile Pro Ala 245 250165238PRTOryza sativa 165Met Ala Val Glu Glu Val Leu Asp Gly Ala Ala Pro Met Leu Ser Ser1 5 10 15Ser Pro Ala Ala Ser Gly Glu Glu Val Gly Ala Arg Lys Pro Gln Gln 20 25 30Arg Cys Gly Gly Ala Glu Gly Trp Ser Lys Arg Lys Arg Ser Arg Arg 35 40 45Arg His Arg Asp Arg Ala Ala Ala Pro Pro Pro His Gly Ser Glu Glu 50 55 60Glu His Leu Ala Leu Ser Leu Leu Met Leu Ala Arg Gly His Arg Asp65 70 75 80Pro Ser Pro Ala Pro Gln Glu Gln His Gly Cys Ser Val Cys Gly Arg 85 90 95Val Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Thr Ser His Arg 100 105 110Pro Arg Thr Pro Pro Thr Met Ala Ala Val Val Val Val Asp Glu Pro 115 120 125Ala Ala Thr Thr Ala Ser Pro Ala Ala Ser Ser Ser Asn Ser Gly Ser 130 135 140Gly Ser Gly Gly Gly Gly Gly Asn Lys Val His Glu Cys Ser Val Cys145 150 155 160Lys Lys Thr Phe Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys 165 170 175His Tyr Glu Gly Pro Ile Gly Ser Gly Gly Gly Ala Ala Val Ala Gly 180 185 190Arg Gly Phe Asp Leu Asn Leu Pro Ala Val Ala Leu Pro Asp Ile Met 195 200 205Thr Glu Arg Cys Leu Pro Ala Ala Ala Glu Glu Glu Glu Val Leu Ser 210 215 220Pro Leu Ala Ser Phe Lys Lys Pro Arg Leu Met Ile Pro Ala225 230 235166261PRTTriticum aestivum 166Met Ser Ser Ser Ala Met Glu Ala Leu His Ala Leu Ile Pro Glu Gln1 5 10 15His Gln Leu Asp Val Glu Ala Ala Ala Ala Val Ser Ser Ala Thr Ser 20 25 30Gly Glu Glu Ser Gly His Val Leu Gln Gly Trp Ala Lys Arg Lys Arg 35 40 45Ser Arg Arg Gln Arg Ser Glu Glu Glu Asn Leu Ala Leu Cys Leu Leu 50 55 60Met Leu Ser Arg Gly Gly Lys Gln Arg Val Gln Ala Pro Gln Pro Glu65 70 75 80Ser Phe Ala Ala Pro Val Pro Ala Glu Phe Lys Cys Ser Val Cys Gly 85 90 95Lys Ser Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Thr Ser His 100 105 110Arg Val Lys Gln Pro Ser Pro Pro Ser Asp Ala Ala Ala Ala Pro Leu 115 120 125Val Ala Leu Pro Ala Val Ala Ala Ile Leu Pro Ser Ala Glu Pro Ala 130 135 140Thr Ser Ser Thr Ala Ala Ser Ser Asp Gly Ala Thr Asn Arg Val His145 150 155 160Arg Cys Ser Ile Cys Gln Lys Glu Phe Pro Thr Gly Gln Ala Leu Gly 165 170 175Gly His Lys Arg Lys His Tyr Asp Gly Gly Val Gly Ala Ala Ala Ser 180 185 190Ser Thr Glu Leu Leu Ala Ala Ala Ala Ala Glu Ser Glu Val Gly Ser 195 200 205Thr Gly Asn Gly Ser Ser Ala Ala Arg Ala Phe Asp Leu Asn Ile Pro 210 215 220Ala Val Pro Glu Phe Val Trp Arg Pro Cys Ala Lys Gly Lys Met Met225 230 235 240Trp Glu Asp Asp Glu Glu Val Gln Ser Pro Leu Ala Phe Lys Lys Pro 245 250 255Arg Leu Leu Thr Ala 260167269PRTOryza sativa 167Met Ser Ser Ala Ser Ser Met Glu Ala Leu His Ala Ala Val Leu Lys1 5 10 15Glu Glu Gln Gln Gln His Glu Val Glu Glu Ala Thr Val Val Thr Ser 20 25 30Ser Ser Ala Thr Ser Gly Glu Glu Gly Gly His Leu Pro Gln Gly Trp 35 40 45Ala Lys Arg Lys Arg Ser Arg Arg Gln Arg Ser Glu Glu Glu Asn Leu 50 55 60Ala Leu Cys Leu Leu Met Leu Ala Arg Gly Gly His His Arg Val Gln65 70 75 80Ala Pro Pro Pro Leu Ser Ala Ser Ala Pro Pro Pro Ala Gly Ala Glu 85 90 95Phe Lys Cys Ser Val Cys Gly Lys Ser Phe Ser Ser Tyr Gln Ala Leu 100 105 110Gly Gly His Lys Thr Ser His Arg Val Lys Leu Pro Thr Pro Pro Ala 115 120 125Ala Pro Val Leu Ala Pro Ala Pro Val Ala Ala Leu Leu Pro Ser Ala 130 135 140Glu Asp Arg Glu Pro Ala Thr Ser Ser Thr Ala Ala Ser Ser Asp Gly145 150 155 160Met Thr Asn Arg Val His Arg Cys Ser Ile Cys Gln Lys Glu Phe Pro 165 170 175Thr Gly Gln Ala Leu Gly Gly His Lys Arg Lys His Tyr Asp Gly Gly 180 185 190Val Gly Ala Gly Ala Gly Ala Ser Ser Thr Glu Leu Leu Ala Thr Val 195 200 205Ala Ala Glu Ser Glu Val Gly Ser Ser Gly Asn Gly Gln Ser Ala Thr 210 215 220Arg Ala Phe Asp Leu Asn Leu Pro Ala Val Pro Glu Phe Val Trp Arg225

230 235 240Pro Cys Ser Lys Gly Lys Lys Met Trp Asp Glu Glu Glu Glu Val Gln 245 250 255Ser Pro Leu Ala Phe Lys Lys Pro Arg Leu Leu Thr Ala 260 265168220PRTOryza sativa 168Met Ala Leu Asp Gly Lys Pro Pro Val Pro Pro Pro Ser Thr Pro Pro1 5 10 15Met Asp Ser Trp Ala Cys Gly Gly Arg Arg Ser Lys Arg Arg Gly Gly 20 25 30Gly Gly Gly Ser Ser Gly Ser Ser Gly Ser Ser Gly Gly Gly Gly Gly 35 40 45Gly Glu Ser Glu Glu Glu Tyr Leu Ala Ala Cys Leu Leu Met Leu Ala 50 55 60His Gly Val Arg Asp Glu Ala Glu Val Val Gly Val Ala Ala Ala Thr65 70 75 80Ala Lys Pro Gln His Gly Tyr Glu Cys Ser Val Cys Gly Lys Val Tyr 85 90 95Gly Ser Tyr Gln Ala Leu Gly Gly His Lys Thr Ser His Arg Lys Pro 100 105 110Pro Ser Pro Ala Ala Glu Pro Ala Ala Gly Glu Glu Pro Ser Ser Gly 115 120 125Gly Val Ala Gly Glu Ala Lys Val His Arg Cys Ser Ile Cys Leu Arg 130 135 140Thr Phe Pro Ser Gly Gln Ala Leu Gly Gly His Lys Arg Leu His Tyr145 150 155 160Glu Gly Gly Ala Val Gly Asp Ala Val Lys Glu Lys Asn Ser Leu Lys 165 170 175Thr Lys Ala Ala Val Ala Thr Ala Val Leu Lys Asp Phe Asp Leu Asn 180 185 190Leu Pro Ala Ala Ala Thr Thr Ala Gly Asp Glu Ala Glu Ser Ser Pro 195 200 205Pro Glu Ala Lys Arg Ala Arg Leu Leu Leu Leu Val 210 215 220169189PRTAegilops speltoides 169Met Ala Val Asp Ala Val Arg Asp Ala Ala Ala Met Val Ser Glu Glu1 5 10 15Glu Glu Glu Gly Gln Leu Arg Cys Asp Glu Gly Trp Gly Lys Arg Arg 20 25 30Arg Pro Arg Arg Gln Arg Gln Arg Ala Pro Ser Glu Glu Glu His Leu 35 40 45Ala Leu Ser Leu Leu Met Leu Ala Arg Gly His Arg Asp Arg His Leu 50 55 60Leu Gly Ser Ser Glu Pro Ala Gln Glu His Arg Cys Ser Val Cys Gly65 70 75 80Lys Gly Phe Pro Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His 85 90 95Arg Pro Lys Pro Ala Pro Ala Gly Ala Asp Glu Pro Ala Ala Thr Thr 100 105 110Ala Ala Ser Pro Ala Ala Ser Ser Ser Thr Thr Ser Ser Gly Ala Gly 115 120 125Gly Gly Gly Arg Val His Glu Cys Ser Val Cys Lys Lys Thr Phe Pro 130 135 140Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu Gly Pro145 150 155 160Ile Gly Ala Thr Val Val Ala Ser Arg Gly Phe Asp Leu Asn Leu Pro 165 170 175Ala Leu Pro Asp Ile Val Thr Glu Arg Glu Arg Cys Met 180 185170184PRTSecale cereale 170Arg Arg Arg Pro Arg Arg Gln Arg Gln Arg Ala Pro Ser Glu Glu Glu1 5 10 15His Leu Ala Leu Ser Leu Leu Met Leu Ala Arg Gly His Arg Asp Arg 20 25 30His Leu Pro Pro Ser Ser Glu Pro Ala Gln Glu His Arg Cys Ser Val 35 40 45Cys Gly Lys Gly Phe Pro Ser Tyr Gln Ala Leu Gly Gly His Lys Ala 50 55 60Ser His Arg Pro Lys Pro Ala Pro Ala Gly Ala Asp Glu Pro Ala Ala65 70 75 80Thr Ala Ala Ala Ser Pro Ala Ala Ser Ser Ser Thr Thr Ser Ser Gly 85 90 95Ala Gly Val Lys Val His Glu Cys Ser Val Cys Lys Lys Thr Phe Pro 100 105 110Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu Gly Pro 115 120 125Ile Gly Gly Gly Gly Ala Pro Ala Val Ala Ser Arg Gly Phe Asp Leu 130 135 140Asn Leu Pro Ala Leu Pro Asp Ile Val Thr Glu Arg Glu Arg Cys Met145 150 155 160Pro Ala Pro Ala Asp Glu Glu Glu Val Leu Ser Pro Leu Ala Phe Lys 165 170 175Lys Pro Arg Leu Met Ile Pro Ala 180171200PRTSaccharum officinarum 171Asp Val Val Ala Ala Ala Ala Asp Gln Val Ala Thr Thr Ser Asn Ser1 5 10 15Ser Gly Thr Ala Ala Glu Glu Asp Lys Asp Val Lys Thr Ala Val Gln 20 25 30Gln Glu His Gly Gln Gly Leu Ala Lys Arg Lys Arg Ser Arg Arg Arg 35 40 45Arg Asp Arg Glu Gln Gln Gln Leu Pro Lys Glu His Pro Thr Gln Glu 50 55 60Glu Tyr Leu Ala Gln Cys Leu Val Met Leu Ala Thr Gly Arg Arg Asp65 70 75 80Gly Asp Val Pro Ala Leu Ala Ser Ala Pro Pro Pro Pro Gln Gly Gln 85 90 95Gln Gln Asp His Ala Cys Ser Val Cys Gly Lys Ala Phe Pro Thr Tyr 100 105 110Gln Ala Leu Gly Gly His Lys Ala Ser His Arg Thr Arg Pro Ser Pro 115 120 125Pro Ser Ala Ala Thr Glu Val Val Gly Asp His His Glu Glu Gln Lys 130 135 140Pro Val Leu Pro Ser Ser Ser Ser Ala Ala Ser Ala Gly Ala Asp Asn145 150 155 160Asn Lys Pro Ala Ala Ala His Glu Cys Asn Val Cys Gly Lys Ala Phe 165 170 175Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg Arg His Tyr Asp Gly 180 185 190Thr Ile Gly Ser Ala Ala Ala Pro 195 200172253PRTNicotiana benthamiana 172Met Ala Leu Glu Ala Leu Asn Ser Pro Thr Thr Thr Thr Pro Pro Thr1 5 10 15Phe Gln Phe Glu Asn Asn Gly Pro Leu Arg Tyr Leu Glu Asn Trp Thr 20 25 30Lys Gly Lys Arg Ser Lys Arg Pro Arg Ser Met Glu Arg Gln Pro Thr 35 40 45Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu Ala Arg Ser Asp 50 55 60Gly Ser Ala Asn Arg Glu Gln Ser Leu Pro Pro Pro Pro Val Pro Val65 70 75 80Met Lys Ile His Ala Pro Pro Glu Glu Lys Met Val Tyr Lys Cys Ser 85 90 95Val Cys Gly Lys Gly Phe Gly Ser Tyr Gln Ala Leu Gly Gly His Lys 100 105 110Ala Ser His Arg Lys Leu Val Ala Gly Gly Gly Gly Gly Asp Asp Gln 115 120 125Ser Thr Thr Ser Thr Thr Thr Asn Ala Thr Gly Thr Thr Ser Ser Ala 130 135 140Asn Gly Asn Gly Asn Gly Ser Gly Lys Thr His Glu Cys Ser Ile Cys145 150 155 160His Lys Arg Phe Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys 165 170 175His Tyr Asp Gly Gly Asn Ser Asn Gly Gly Val Ser Val Ser Ala Ser 180 185 190Val Gly Leu Thr Ser Ser Glu Gly Val Gly Ser Thr Val Ser His Arg 195 200 205Asp Phe Asp Leu Asn Ile Pro Ala Leu Pro Glu Phe Trp Pro Gly Phe 210 215 220Gly Ser Gly Glu Asp Glu Val Glu Ser Pro His Pro Thr Lys Lys Ser225 230 235 240Arg Leu Ser Leu Pro Pro Lys Phe Glu Leu Phe Arg Glu 245 250173261PRTCapsicum annuum 173Met Ala Leu Glu Ala Leu Asn Ser Pro Thr Gly Thr Pro Thr Pro Pro1 5 10 15Pro Phe Gln Phe Glu Ser Asp Gly Gln Gln Leu Arg Tyr Ile Glu Asn 20 25 30Trp Arg Lys Gly Lys Arg Ser Lys Arg Ser Arg Ser Met Glu His Gln 35 40 45Pro Thr Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu Ala Arg 50 55 60Ser Gly Gly Ser Val Asn His Gln Arg Ser Leu Pro Pro Pro Ala Pro65 70 75 80Val Met Lys Leu His Ala Pro Ser Ser Ser Ser Ala Ala Glu Glu Glu 85 90 95Lys Glu Lys Met Val Tyr Lys Cys Ser Val Cys Gly Lys Gly Phe Gly 100 105 110Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg Lys Leu Val 115 120 125Pro Gly Gly Asp Asp Gln Ser Thr Thr Ser Thr Thr Thr Asn Ala Thr 130 135 140Gly Thr Thr Thr Ser Val Asn Gly Asn Gly Asn Arg Ser Gly Arg Thr145 150 155 160His Glu Cys Ser Ile Cys His Lys Cys Phe Pro Thr Gly Gln Ala Leu 165 170 175Gly Gly His Lys Arg Cys His Tyr Asp Gly Gly Ile Gly Asn Gly Asn 180 185 190Ala Asn Ser Gly Val Ser Ala Ser Val Gly Val Thr Ser Ser Glu Gly 195 200 205Val Gly Ser Thr Val Ser His Arg Asp Phe Asp Leu Asn Ile Pro Ala 210 215 220Leu Pro Glu Phe Trp Leu Gly Phe Gly Ser Gly Glu Asp Glu Val Glu225 230 235 240Ser Pro His Pro Ala Lys Lys Ser Arg Leu Cys Leu Pro Pro Lys Tyr 245 250 255Glu Leu Phe Gln His 260174235PRTMedicago sativa 174Met Ala Met Glu Ala Leu Asn Ser Pro Thr Thr Ala Thr Pro Phe Thr1 5 10 15Pro Phe Glu Glu Pro Asn Leu Ser Tyr Leu Glu Thr Pro Trp Thr Lys 20 25 30Gly Lys Arg Ser Lys Arg Ser Arg Met Asp Gln Ser Ser Cys Thr Glu 35 40 45Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu Ala Arg Ser Gly Asn 50 55 60Asn Asn Asp Lys Lys Ser Asp Ser Val Ala Thr Pro Leu Thr Thr Val65 70 75 80Lys Leu Ser His Lys Cys Ser Val Cys Asn Lys Ala Phe Ser Ser Tyr 85 90 95Gln Ala Leu Gly Gly His Lys Ala Ser His Arg Lys Ala Val Met Ser 100 105 110Ala Thr Thr Ala Glu Asp Gln Ile Thr Thr Thr Ser Ser Ala Val Thr 115 120 125Thr Ser Ser Ala Ser Asn Gly Lys Asn Lys Thr His Glu Cys Ser Ile 130 135 140Cys His Lys Ser Phe Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg145 150 155 160Cys His Tyr Glu Gly Ser Val Gly Ala Gly Ala Gly Ala Gly Ser Asn 165 170 175Ala Val Thr Ala Ser Glu Gly Val Gly Leu Ser His Ser His His Arg 180 185 190Asp Phe Asp Leu Asn Leu Pro Ala Phe Pro Asp Phe Ser Lys Lys Phe 195 200 205Phe Val Asp Asp Glu Val Phe Ser Pro Leu Pro Ala Ala Lys Lys Pro 210 215 220Cys Leu Phe Lys Leu Glu Ile Pro Ser His Tyr225 230 235175240PRTGlycine max 175Met Ala Leu Glu Ala Leu Asn Ser Pro Thr Thr Thr Ala Pro Ser Phe1 5 10 15Pro Phe Asp Asp Pro Thr Ile Pro Trp Ala Lys Arg Lys Arg Ser Lys 20 25 30Arg Ser Arg Asp His Pro Ser Glu Glu Glu Tyr Leu Ala Leu Cys Leu 35 40 45Ile Met Leu Ala Arg Gly Gly Thr Thr Thr Val Asn Asn Arg His Val 50 55 60Ser Pro Pro Pro Leu Gln Pro Gln Pro Gln Pro Thr Pro Asp Pro Ser65 70 75 80Thr Lys Leu Ser Tyr Lys Cys Ser Val Cys Asp Lys Ser Phe Pro Ser 85 90 95Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg Lys Leu Ala Gly 100 105 110Ala Ala Glu Asp Gln Pro Pro Ser Thr Thr Thr Ser Ser Ala Ala Ala 115 120 125Thr Ser Ser Ala Ser Gly Gly Lys Ala His Glu Cys Ser Ile Cys His 130 135 140Lys Ser Phe Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys His145 150 155 160Tyr Glu Gly Asn Gly Asn Gly Asn Asn Asn Asn Ser Asn Ser Val Val 165 170 175Thr Val Ala Ser Glu Gly Val Gly Ser Thr His Thr Val Ser His Gly 180 185 190His His Arg Asp Phe Asp Leu Asn Ile Pro Ala Phe Pro Asp Phe Ser 195 200 205Thr Lys Val Gly Glu Asp Glu Val Glu Ser Pro His Pro Val Met Lys 210 215 220Lys Pro Arg Leu Phe Val Ile Pro Lys Ile Glu Ile Pro Gln Phe Gln225 230 235 240176265PRTArabidopsis thaliana 176Met Val Ala Tyr Pro Thr Lys Lys Thr Ala Ile Lys Gln Phe Leu His1 5 10 15Cys Asn Ser Gln Ala Thr Phe Lys Leu Lys Leu Glu Arg Gln Glu Ile 20 25 30Leu Arg Ile Phe Asn Leu Met Ala Leu Glu Ala Leu Thr Ser Pro Arg 35 40 45Leu Ala Ser Pro Ile Pro Pro Leu Phe Glu Asp Ser Ser Val Phe His 50 55 60Gly Val Glu His Trp Thr Lys Gly Lys Arg Ser Lys Arg Ser Arg Ser65 70 75 80Asp Phe His His Gln Asn Leu Thr Glu Glu Glu Tyr Leu Ala Phe Cys 85 90 95Leu Met Leu Leu Ala Arg Asp Asn Arg Gln Pro Pro Pro Pro Pro Ala 100 105 110Val Glu Lys Leu Ser Tyr Lys Cys Ser Val Cys Asp Lys Thr Phe Ser 115 120 125Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg Lys Asn Leu 130 135 140Ser Gln Thr Leu Ser Gly Gly Gly Asp Asp His Ser Thr Ser Ser Ala145 150 155 160Thr Thr Thr Ser Ala Val Thr Thr Gly Ser Gly Lys Ser His Val Cys 165 170 175Thr Ile Cys Asn Lys Ser Phe Pro Ser Gly Gln Ala Leu Gly Gly His 180 185 190Lys Arg Cys His Tyr Glu Gly Asn Asn Asn Ile Asn Thr Ser Ser Val 195 200 205Ser Asn Ser Glu Gly Ala Gly Ser Thr Ser His Val Ser Ser Ser His 210 215 220Arg Gly Phe Asp Leu Asn Ile Pro Pro Ile Pro Glu Phe Ser Met Val225 230 235 240Asn Gly Asp Asp Glu Val Met Ser Pro Met Pro Ala Lys Lys Pro Arg 245 250 255Phe Asp Phe Pro Val Lys Leu Gln Leu 260 265177227PRTArabidopsis thaliana 177Met Ala Leu Glu Ala Leu Thr Ser Pro Arg Leu Ala Ser Pro Ile Pro1 5 10 15Pro Leu Phe Glu Asp Ser Ser Val Phe His Gly Val Glu His Trp Thr 20 25 30Lys Gly Lys Arg Ser Lys Arg Ser Arg Ser Asp Phe His His Gln Asn 35 40 45Leu Thr Glu Glu Glu Tyr Leu Ala Phe Cys Leu Met Leu Leu Ala Arg 50 55 60Asp Asn Arg Gln Pro Pro Pro Pro Pro Ala Val Glu Lys Leu Ser Tyr65 70 75 80Lys Cys Ser Val Cys Asp Lys Thr Phe Ser Ser Tyr Gln Ala Leu Gly 85 90 95Gly His Lys Ala Ser His Arg Lys Asn Leu Ser Gln Thr Leu Ser Gly 100 105 110Gly Gly Asp Asp His Ser Thr Ser Ser Ala Thr Thr Thr Ser Ala Val 115 120 125Thr Thr Gly Ser Gly Lys Ser His Val Cys Thr Ile Cys Asn Lys Ser 130 135 140Phe Pro Ser Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu145 150 155 160Gly Asn Asn Asn Ile Asn Thr Ser Ser Val Ser Asn Ser Glu Gly Ala 165 170 175Gly Ser Thr Ser His Val Ser Ser Ser His Arg Gly Phe Asp Leu Asn 180 185 190Ile Pro Pro Ile Pro Glu Phe Ser Met Val Asn Gly Asp Asp Glu Val 195 200 205Met Ser Pro Met Pro Ala Lys Lys Pro Arg Phe Asp Phe Pro Val Lys 210 215 220Leu Gln Leu225178247PRTDatisca glomerata 178Met Ala Leu Glu Ala Leu Asn Ser Pro Thr Thr Ala Thr Pro Val Phe1 5 10 15His Tyr Asp Asp Pro Ser Leu Asn Tyr Leu Glu Pro Trp Thr Lys Arg 20 25 30Lys Arg Ser Lys Arg Thr Arg Leu Asp Ser Pro His Thr Glu Glu Glu 35 40 45Tyr Leu Ala Phe Cys Leu Ile Met Leu Ala Arg Gly Arg Val Ala Ser 50 55 60Ala Asn Arg Arg Asp Ser Gln Ser Ser Ile Gln Ile Gln Pro Glu Ala65 70 75 80Thr Thr Ser Ala Thr Lys Val Ser Tyr Lys Cys Ser Val Cys Asp Lys 85 90 95Ala Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg 100 105 110Lys Leu Ala Gly Gly Glu Asp Gln Ser Thr Ser Phe Ala Thr Thr Asn 115 120 125Ser Ala

Thr Val Thr Thr Thr Thr Ala Ser Gly Gly Gly Gly Arg Ser 130 135 140His Glu Cys Ser Ile Cys His Lys Ser Phe Pro Thr Gly Gln Ala Leu145 150 155 160Gly Gly His Lys Arg Cys His Tyr Glu Gly Ser Ile Gly Gly Asn Ser 165 170 175Ile His His His Asn Asn Thr Thr Asn Ser Gly Ser Asn Gly Gly Met 180 185 190Ser Met Thr Ser Glu Val Gly Ser Thr His Thr Val Ser His Ser His 195 200 205Arg Asp Phe Asp Leu Asn Ile Pro Ala Leu Pro Glu Phe Arg Ser Asn 210 215 220Phe Phe Ile Ser Gly Asp Asp Glu Val Glu Ser Pro His Pro Ala Lys225 230 235 240Lys Pro Arg Ile Leu Met Lys 245179227PRTArabidopsis thaliana 179Met Ala Leu Glu Ala Leu Thr Ser Pro Arg Leu Ala Ser Pro Ile Pro1 5 10 15Pro Leu Phe Glu Asp Ser Ser Val Phe His Gly Val Glu His Trp Thr 20 25 30Lys Gly Lys Arg Ser Lys Arg Ser Arg Ser Asp Phe His His Gln Asn 35 40 45Leu Thr Glu Glu Glu Tyr Leu Ala Phe Trp Leu Met Leu Leu Ala Arg 50 55 60Asp Asn Arg Gln Pro Pro Pro Pro Pro Ala Val Glu Lys Leu Ser Tyr65 70 75 80Lys Cys Ser Val Cys Asp Lys Thr Phe Ser Ser Tyr Gln Ala Leu Gly 85 90 95Gly His Lys Ala Ser His Arg Lys Asn Leu Ser Gln Thr Leu Ser Gly 100 105 110Gly Gly Asp Asp His Ser Thr Ser Ser Ala Thr Thr Thr Ser Ala Val 115 120 125Thr Thr Gly Ser Gly Lys Ser His Val Cys Thr Ile Cys Asn Lys Ser 130 135 140Phe Pro Ser Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu145 150 155 160Gly Asn Asn Asn Ile Asn Thr Ser Ser Val Ser Asn Ser Glu Gly Ala 165 170 175Gly Ser Thr Ser His Val Ser Ser Ser His Arg Gly Phe Asp Leu Asn 180 185 190Ile Pro Pro Ile Pro Glu Phe Ser Met Val Asn Gly Asp Asp Glu Val 195 200 205Met Ser Pro Met Pro Ala Lys Lys Pro Arg Phe Asp Phe Pro Val Lys 210 215 220Leu Gln Leu225180253PRTPetunia x hybrida 180Met Ala Leu Glu Ala Leu Asn Ser Pro Thr Thr Thr Thr Pro Pro Ser1 5 10 15Phe Gln Phe Glu Asn Asn Gly Leu Lys Tyr Leu Glu Ser Trp Thr Lys 20 25 30Gly Lys Arg Ser Lys Arg Gln Arg Ser Met Glu Arg Gln Cys Thr Glu 35 40 45Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu Ala Arg Ser Asp Gly 50 55 60Ser Val Asn Asn Ser Arg Ser Leu Pro Pro Pro Pro Leu Pro Pro Ser65 70 75 80Val Pro Val Thr Ser Gln Ile Asn Ala Thr Leu Leu Glu Gln Lys Asn 85 90 95Leu Tyr Lys Cys Ser Val Cys Gly Lys Gly Phe Gly Ser Tyr Gln Ala 100 105 110Leu Gly Gly His Lys Ala Ser His Arg Lys Leu Val Ser Met Gly Gly 115 120 125Asp Glu Gln Ser Thr Thr Ser Thr Thr Thr Asn Val Thr Gly Thr Ser 130 135 140Ser Ala Asn Val Asn Gly Asn Gly Arg Thr His Glu Cys Ser Ile Cys145 150 155 160His Lys Cys Phe Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys 165 170 175His Tyr Asp Gly Gly Asn Gly Asn Gly Asn Gly Ser Val Ser Val Gly 180 185 190Val Thr Ser Ser Glu Gly Val Gly Ser Thr Ile Ser His His Arg Asp 195 200 205Phe Asp Leu Asn Ile Pro Ala Leu Pro Glu Phe Trp Pro Gly Phe Gly 210 215 220Ser Gly Glu Asp Glu Val Glu Ser Pro His Pro Ala Lys Lys Ser Arg225 230 235 240Leu Ser Leu Pro Pro Lys Leu Glu Leu Phe Lys Gly Leu 245 250181273PRTArabidopsis thaliana 181Met Ala Leu Glu Ala Met Asn Thr Pro Thr Ser Ser Phe Thr Arg Ile1 5 10 15Glu Thr Lys Glu Asp Leu Met Asn Asp Ala Val Phe Ile Glu Pro Trp 20 25 30Leu Lys Arg Lys Arg Ser Lys Arg Gln Arg Ser His Ser Pro Ser Ser 35 40 45Ser Ser Ser Ser Pro Pro Arg Ser Arg Pro Lys Ser Gln Asn Gln Asp 50 55 60Leu Thr Glu Glu Glu Tyr Leu Ala Leu Cys Leu Leu Met Leu Ala Lys65 70 75 80Asp Gln Pro Ser Gln Thr Arg Phe His Gln Gln Ser Gln Ser Leu Thr 85 90 95Pro Pro Pro Glu Ser Lys Asn Leu Pro Tyr Lys Cys Asn Val Cys Glu 100 105 110Lys Ala Phe Pro Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His 115 120 125Arg Ile Lys Pro Pro Thr Val Ile Ser Thr Thr Ala Asp Asp Ser Thr 130 135 140Ala Pro Thr Ile Ser Ile Val Ala Gly Glu Lys His Pro Ile Ala Ala145 150 155 160Ser Gly Lys Ile His Glu Cys Ser Ile Cys His Lys Val Phe Pro Thr 165 170 175Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu Gly Asn Leu 180 185 190Gly Gly Gly Gly Gly Gly Gly Ser Lys Ser Ile Ser His Ser Gly Ser 195 200 205Val Ser Ser Thr Val Ser Glu Glu Arg Ser His Arg Gly Phe Ile Asp 210 215 220Leu Asn Leu Pro Ala Leu Pro Glu Leu Ser Leu His His Asn Pro Ile225 230 235 240Val Asp Glu Glu Ile Leu Ser Pro Leu Thr Gly Lys Lys Pro Leu Leu 245 250 255Leu Thr Asp His Asp Gln Val Ile Lys Lys Glu Asp Leu Ser Leu Lys 260 265 270Ile182238PRTArabidopsis thaliana 182Met Ala Leu Glu Thr Leu Thr Ser Pro Arg Leu Ser Ser Pro Met Pro1 5 10 15Thr Leu Phe Gln Asp Ser Ala Leu Gly Phe His Gly Ser Lys Gly Lys 20 25 30Arg Ser Lys Arg Ser Arg Ser Glu Phe Asp Arg Gln Ser Leu Thr Glu 35 40 45Asp Glu Tyr Ile Ala Leu Cys Leu Met Leu Leu Ala Arg Asp Gly Asp 50 55 60Arg Asn Arg Asp Leu Asp Leu Pro Ser Ser Ser Ser Ser Pro Pro Leu65 70 75 80Leu Pro Pro Leu Pro Thr Pro Ile Tyr Lys Cys Ser Val Cys Asp Lys 85 90 95Ala Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg 100 105 110Lys Ser Phe Ser Leu Thr Gln Ser Ala Gly Gly Asp Glu Leu Ser Thr 115 120 125Ser Ser Ala Ile Thr Thr Ser Gly Ile Ser Gly Gly Gly Gly Gly Ser 130 135 140Val Lys Ser His Val Cys Ser Ile Cys His Lys Ser Phe Ala Thr Gly145 150 155 160Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu Gly Lys Asn Gly 165 170 175Gly Gly Val Ser Ser Ser Val Ser Asn Ser Glu Asp Val Gly Ser Thr 180 185 190Ser His Val Ser Ser Gly His Arg Gly Phe Asp Leu Asn Ile Pro Pro 195 200 205Ile Pro Glu Phe Ser Met Val Asn Gly Asp Glu Glu Val Met Ser Pro 210 215 220Met Pro Ala Lys Lys Leu Arg Phe Asp Phe Pro Gly Lys Pro225 230 235183238PRTArabidopsis thaliana 183Met Ala Leu Glu Thr Leu Thr Ser Pro Arg Leu Ser Ser Pro Met Pro1 5 10 15Thr Leu Phe Gln Asp Ser Ala Leu Gly Phe His Gly Ser Lys Gly Lys 20 25 30Arg Ser Lys Arg Ser Arg Ser Glu Phe Asp Arg Gln Ser Leu Thr Glu 35 40 45Asp Glu Tyr Ile Ala Leu Cys Leu Met Leu Leu Ala Arg Asp Gly Asp 50 55 60Arg Asn Arg Asp Leu Asp Leu Pro Ser Ser Ser Ser Ser Pro Pro Leu65 70 75 80Leu Pro Pro Leu Pro Thr Pro Ile Tyr Lys Cys Ser Val Cys Asp Lys 85 90 95Ala Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg 100 105 110Lys Ser Phe Ser Leu Thr Gln Ser Ala Gly Gly Asp Glu Leu Ser Thr 115 120 125Ser Ser Ala Ile Thr Thr Ser Gly Ile Ser Gly Gly Gly Gly Gly Ser 130 135 140Val Lys Ser His Val Cys Ser Ile Cys His Lys Ser Phe Ala Thr Gly145 150 155 160Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu Gly Lys Asn Gly 165 170 175Gly Gly Val Ser Ser Ser Val Ser Asn Ser Glu Asp Val Gly Ser Thr 180 185 190Ser His Val Ser Ser Gly His Arg Gly Phe Asp Leu Asn Ile Pro Pro 195 200 205Ile Pro Glu Phe Ser Met Val Asn Gly Asp Glu Glu Val Met Ser Pro 210 215 220Met Pro Ala Lys Lys Leu Arg Phe Asp Phe Pro Glu Lys Pro225 230 235184235PRTBrassica rapa 184Met Ala Leu Glu Thr Leu Asn Ser Pro Thr Ser Ala Thr Ala Ser Ala1 5 10 15Arg Pro Leu Leu Arg Tyr Arg Glu Glu Met Glu Pro Glu Asn Leu Glu 20 25 30Gln Trp Ala Lys Arg Lys Arg Thr Lys Arg Gln Arg Phe Asp Gln Ser 35 40 45Arg Leu Asn Gln Glu Thr Ala Pro Ser Glu Glu Glu Tyr Leu Ala Leu 50 55 60Cys Leu Leu Met Leu Ala Arg Gly Ser Ala Val Gln Ser Pro Leu Pro65 70 75 80Pro Ser Ser Ser Ser Asp His Arg Gly Tyr Lys Cys Thr Val Cys Gly 85 90 95Lys Ser Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Thr Ser His 100 105 110Arg Lys Pro Ala Ser Asn Val Asn Val Pro Ile Asn Gln Glu Gln Ser 115 120 125Asn Asn Ser His Ser Asn Ser Asn Gly Gly Ser Val Val Ile Asn Gly 130 135 140Asn Gly Val Ser Gln Ser Gly Lys Ile His Thr Cys Ser Ile Cys Phe145 150 155 160Lys Ser Phe Ser Ser Gly Gln Ala Leu Gly Gly His Lys Arg Cys His 165 170 175Tyr Asp Ala Gly Asn Asn Gly Asn Gly Asn Gly Ser Ser Ser Asn Ser 180 185 190Val Glu Val Val Gly Gly Ser Asp Gly Ser Tyr Val Asp Asp Glu Arg 195 200 205Ser Ser Glu Gln Ser Ala Thr Gly Asp Asn Arg Gly Phe Asp Leu Asn 210 215 220Leu Pro Ala Asp Gln Val Ala Val Val Ile Ser225 230 235185237PRTBrassica rapa 185Met Ala Leu Glu Thr Leu Asn Ser Pro Thr Ser Ala Thr Ala Ser Ala1 5 10 15Arg Pro Leu Leu Arg Tyr Arg Glu Glu Met Glu Pro Glu Asn Leu Glu 20 25 30Gln Trp Ala Lys Arg Lys Arg Thr Lys Arg Gln Arg Phe Asp Gln Ser 35 40 45Arg Leu Asn Gln Glu Thr Ala Pro Ser Glu Glu Glu Tyr Leu Ala Leu 50 55 60Cys Leu Leu Met Leu Ala Arg Gly Ser Ala Val Gln Ser Pro Leu Pro65 70 75 80Pro Ser Ser Ser Ser Asp His Arg Gly Tyr Lys Cys Thr Val Cys Gly 85 90 95Lys Ser Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Thr Ser His 100 105 110Arg Lys Pro Ala Ser Asn Val Asn Val Pro Ile Asn Gln Glu Gln Ser 115 120 125Asn Asn Ser His Ser Asn Ser Asn Gly Gly Ser Val Ala Ile Asn Gly 130 135 140Asn Gly Val Ser Gln Ser Gly Lys Ile His Thr Cys Ser Ile Cys Phe145 150 155 160Lys Ser Phe Ser Ser Gly Gln Ala Leu Gly Gly His Lys Arg Cys His 165 170 175Tyr Asp Ala Gly Ile Asn Gly Asn Gly Asn Gly Ser Ser Ser Asn Ser 180 185 190Val Glu Val Val Gly Gly Ser Asp Gly Asn Tyr Val Asp Asp Glu Arg 195 200 205Ser Ser Glu Gln Ser Ala Thr Gly Asp Asn Arg Gly Phe Asp Leu Asn 210 215 220Leu Pro Ala Asp Gln Val Ala Val Val Ile Ser Lys Arg225 230 235186273PRTNicotiana tabacum 186Met Thr Leu Glu Ala Leu Lys Ser Pro Thr Ala Ala Thr Pro Thr Leu1 5 10 15Pro Pro Arg Tyr Glu Asp Asp Asp Glu Ile His Asn Leu Asp Ser Trp 20 25 30Ala Lys Gly Lys Arg Ser Lys Arg Pro Arg Ile Asp Ala Pro Pro Thr 35 40 45Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu Ala Arg Ser Gly 50 55 60Thr Gly Thr Arg Thr Gly Leu Thr Asp Ala Thr Thr Ser Gln Gln Pro65 70 75 80Ala Asp Lys Lys Thr Ala Glu Leu Pro Pro Val His Lys Lys Glu Val 85 90 95Ala Thr Glu Gln Ala Glu Gln Ser Tyr Lys Cys Ser Val Cys Asp Lys 100 105 110Ala Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg 115 120 125Lys Thr Thr Thr Thr Ala Thr Ala Ala Ser Asp Asp Asn Asn Pro Ser 130 135 140Thr Ser Thr Ser Thr Gly Ala Val Asn Ile Ser Ala Leu Asn Pro Thr145 150 155 160Gly Arg Ser His Val Cys Ser Ile Cys His Lys Ala Phe Pro Thr Gly 165 170 175Gln Ala Leu Gly Gly His Lys Arg Arg His Tyr Glu Gly Lys Leu Gly 180 185 190Gly Asn Ser Arg Asp Leu Gly Gly Gly Gly Gly Gly Gly His Ser Gly 195 200 205Ser Val Leu Thr Thr Ser Asp Gly Gly Ala Ser Thr His Thr Leu Arg 210 215 220Asp Phe Asp Leu Asn Met Pro Ala Ser Pro Glu Leu Gln Leu Gly Leu225 230 235 240Ser Ile Asp Cys Gly Arg Lys Ser Gln Leu Leu Pro Met Val Gln Glu 245 250 255Val Glu Ser Pro Met Pro Ala Lys Lys Pro Arg Leu Leu Phe Ser Leu 260 265 270Gly 187274PRTPetunia x hybrida 187Met Ala Leu Glu Ala Leu Lys Ser Pro Thr Ala Ala Thr Pro Ser Leu1 5 10 15Pro Pro Arg Tyr Glu Asp His Val Asp Met Asn Asn Leu Asp Ser Trp 20 25 30Val Lys Gly Lys Arg Ser Lys Arg Pro Arg Ile Glu Thr Pro Pro Ser 35 40 45Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu Ala Arg Ser Gly 50 55 60Asn Gly Thr Thr Pro Gly Ser Thr Asp Thr Thr Ile Thr Thr Thr Ile65 70 75 80Ser Lys Glu Pro Glu Lys Lys Asn Arg Glu Leu Thr Pro Val His Gln 85 90 95Glu Thr Glu Gln Ser Tyr Lys Cys Ser Val Cys Asp Lys Ser Phe Ser 100 105 110Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg Lys Ile Thr 115 120 125Thr Ile Ala Thr Thr Ala Leu Leu Asp Asp Asn Asn Asn Asn Pro Thr 130 135 140Thr Ser Asn Ser Thr Ser Gly Asn Val Val Asn Asn Ile Ser Ala Leu145 150 155 160Asn Pro Ser Gly Arg Ser His Val Cys Ser Ile Cys His Lys Ala Phe 165 170 175Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg Arg His Tyr Glu Gly 180 185 190Lys Leu Gly Gly Asn Asn Asn Asn His Arg Asp Gly Gly Gly His Ser 195 200 205Gly Ser Val Val Thr Thr Ser Asp Gly Gly Ala Ser Thr His Thr Leu 210 215 220Arg Asp Phe Asp Leu Asn Met Leu Pro Pro Ser Pro Glu Leu Gln Leu225 230 235 240Gly Leu Ser Ile Asp Cys Asp Leu Lys Ser Gln Ile Pro Ile Glu Gln 245 250 255Glu Val Glu Ser Pro Met Pro Leu Lys Lys Pro Arg Leu Leu Phe Ser 260 265 270Met Asp 188193PRTArabidopsis thaliana 188Met Ala Leu Glu Ala Leu Asn Ser Pro Arg Leu Val Glu Asp Pro Leu1 5 10 15Arg Phe Asn Gly Val Glu Gln Trp Thr Lys Cys Lys Lys Arg Ser Lys 20 25 30Arg Ser Arg Ser Asp Leu His His Asn His Arg Leu Thr Glu Glu Glu 35 40 45Tyr Leu Ala Phe Cys Leu Met Leu Leu Ala Arg Asp Gly Gly Asp Leu 50 55 60Asp Ser Val Thr Val Ala Glu Lys Pro Ser Tyr Lys Cys Gly Val Cys65 70 75 80Tyr Lys Thr Phe Ser Ser

Tyr Gln Ala Leu Gly Gly His Lys Ala Ser 85 90 95His Arg Ser Leu Tyr Gly Gly Gly Glu Asn Asp Lys Ser Thr Pro Ser 100 105 110Thr Ala Val Lys Ser His Val Cys Ser Val Cys Gly Lys Ser Phe Ala 115 120 125Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Asp Gly Gly 130 135 140Val Ser Asn Ser Glu Gly Val Gly Ser Thr Ser His Val Ser Ser Ser145 150 155 160Ser His Arg Gly Phe Asp Leu Asn Ile Ile Pro Val Gln Gly Phe Ser 165 170 175Pro Asp Asp Glu Val Met Ser Pro Met Ala Thr Lys Lys Pro Arg Leu 180 185 190Lys189193PRTArabidopsis thaliana 189Met Ala Leu Glu Ala Leu Asn Ser Pro Arg Leu Val Glu Asp Pro Leu1 5 10 15Arg Phe Asn Gly Val Glu Gln Trp Thr Lys Cys Lys Lys Arg Ser Lys 20 25 30Arg Ser Arg Ser Asp Leu His His Asn His Arg Leu Thr Glu Glu Glu 35 40 45Tyr Leu Ala Phe Cys Leu Met Leu Leu Ala Arg Asp Gly Gly Asp Leu 50 55 60Asp Ser Val Thr Val Glu Glu Lys Pro Ser Tyr Lys Cys Gly Val Cys65 70 75 80Tyr Lys Thr Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser 85 90 95His Arg Ser Leu Tyr Gly Gly Gly Asp Asn Asp Lys Ser Thr Pro Ser 100 105 110Thr Ala Val Lys Ser His Val Cys Ser Val Cys Gly Lys Ser Phe Ala 115 120 125Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Asp Gly Gly 130 135 140Val Ser Asn Ser Glu Gly Val Gly Ser Thr Ser His Val Ser Ser Ser145 150 155 160Ser His Arg Gly Phe Asp Leu Asn Ile Leu Pro Val Gln Gly Phe Ser 165 170 175Arg Asp Asp Glu Val Met Ser Pro Met Ala Thr Lys Lys Pro Arg Leu 180 185 190Lys190215PRTArabidopsis thaliana 190Met Ala Leu Asp Thr Leu Asn Ser Pro Thr Ser Thr Thr Thr Thr Thr1 5 10 15Ala Pro Pro Pro Phe Leu Arg Cys Leu Asp Glu Thr Glu Pro Glu Asn 20 25 30Leu Glu Ser Trp Thr Lys Arg Lys Arg Thr Lys Arg His Arg Ile Asp 35 40 45Gln Pro Asn Pro Pro Pro Ser Glu Glu Glu Tyr Leu Ala Leu Cys Leu 50 55 60Leu Met Leu Ala Arg Gly Ser Ser Asp His His Ser Pro Pro Ser Asp65 70 75 80His His Ser Leu Ser Pro Leu Ser Asp His Gln Lys Asp Tyr Lys Cys 85 90 95Ser Val Cys Gly Lys Ser Phe Pro Ser Tyr Gln Ala Leu Gly Gly His 100 105 110Lys Thr Ser His Arg Lys Pro Val Ser Val Asp Val Asn Asn Ser Asn 115 120 125Gly Thr Val Thr Asn Asn Gly Asn Ile Ser Asn Gly Leu Val Gly Gln 130 135 140Ser Gly Lys Thr His Asn Cys Ser Ile Cys Phe Lys Ser Phe Pro Ser145 150 155 160Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Asp Gly Gly Asn 165 170 175Gly Asn Ser Asn Gly Asp Asn Ser His Lys Phe Asp Leu Asn Leu Pro 180 185 190Ala Asp Gln Val Ser Asp Glu Thr Ile Gly Lys Ser Gln Leu Ser Gly 195 200 205Glu Glu Thr Lys Ser Val Leu 210 215191277PRTPetunia x hybrida 191Met Ala Leu Glu Ala Leu Lys Ser Pro Thr Ala Ala Thr Pro Thr Leu1 5 10 15Pro Pro Arg Tyr Glu Asp Gln Val Asp Met Ser Asn Leu Asp Ser Trp 20 25 30Val Lys Gly Lys Arg Ser Lys Arg Pro Arg Ile Glu Thr Pro Pro Ser 35 40 45Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu Ala Arg Ser Gly 50 55 60Asn Gly Thr Thr Pro Ser Ser Ile Pro Gly Ser Thr Asp Thr Thr Thr65 70 75 80Ile Ser Lys Glu Pro Glu Lys Lys Asn Arg Asp Val Ala Pro Val Tyr 85 90 95Gln Glu Thr Glu Gln Ser Tyr Lys Cys Ser Val Cys Asp Lys Ser Phe 100 105 110Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg Lys Ile 115 120 125Thr Thr Ile Ala Thr Thr Ala Leu Leu Asp Asp Asn Asn Asn Asn Pro 130 135 140Thr Thr Ser Asn Ser Thr Asn Gly Asn Val Val Asn Asn Ile Ser Thr145 150 155 160Leu Asn Pro Ser Gly Arg Ser His Val Cys Ser Ile Cys His Lys Ala 165 170 175Phe Pro Ser Gly Gln Ala Leu Gly Gly His Lys Arg Arg His Tyr Glu 180 185 190Gly Lys Leu Gly Gly Asn Asn Asn Asn Asn His Arg Asp Gly Gly Gly 195 200 205His Ser Gly Ser Val Val Thr Thr Ser Asp Gly Gly Ala Ser Thr His 210 215 220Thr Leu Arg Asp Phe Asp Leu Asn Met Leu Pro Pro Ser Pro Glu Leu225 230 235 240Gln Leu Gly Leu Ser Ile Asp Cys Gly Leu Lys Ser Gln Gln Val Pro 245 250 255Ile Glu Gln Glu Val Glu Ser Pro Met Pro Leu Lys Lys Pro Arg Leu 260 265 270Leu Phe Ser Met Asp 275192245PRTArabidopsis thaliana 192Met Ala Leu Glu Thr Leu Asn Ser Pro Thr Ala Thr Thr Thr Ala Arg1 5 10 15Pro Leu Leu Arg Tyr Arg Glu Glu Met Glu Pro Glu Asn Leu Glu Gln 20 25 30Trp Ala Lys Arg Lys Arg Thr Lys Arg Gln Arg Phe Asp His Gly His 35 40 45Gln Asn Gln Glu Thr Asn Lys Asn Leu Pro Ser Glu Glu Glu Tyr Leu 50 55 60Ala Leu Cys Leu Leu Met Leu Ala Arg Gly Ser Ala Val Gln Ser Pro65 70 75 80Pro Leu Pro Pro Leu Pro Ser Arg Ala Ser Pro Ser Asp His Arg Asp 85 90 95Tyr Lys Cys Thr Val Cys Gly Lys Ser Phe Ser Ser Tyr Gln Ala Leu 100 105 110Gly Gly His Lys Thr Ser His Arg Lys Pro Thr Asn Thr Ser Ile Thr 115 120 125Ser Gly Asn Gln Glu Leu Ser Asn Asn Ser His Ser Asn Ser Gly Ser 130 135 140Val Val Ile Asn Val Thr Val Asn Thr Gly Asn Gly Val Ser Gln Ser145 150 155 160Gly Lys Ile His Thr Cys Ser Ile Cys Phe Lys Ser Phe Ala Ser Gly 165 170 175Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Asp Gly Gly Asn Asn 180 185 190Gly Asn Gly Asn Gly Ser Ser Ser Asn Ser Val Glu Leu Val Ala Gly 195 200 205Ser Asp Val Ser Asp Val Asp Asn Glu Arg Trp Ser Glu Glu Ser Ala 210 215 220Ile Gly Gly His Arg Gly Phe Asp Leu Asn Leu Pro Ala Asp Gln Val225 230 235 240Ser Val Thr Thr Ser 245193248PRTSolanum tuberosum 193Ile Glu Met Ala Leu Glu Ala Leu Asn Ser Pro Thr Gly Thr Ser Asn1 5 10 15Pro Gln Thr Phe Lys Phe Glu Ser Lys Gly Gln Gln Gln Leu Arg Tyr 20 25 30Leu Glu Asn Trp Thr Lys Gly Lys Arg Ser Lys Arg Ser Arg Ser Met 35 40 45Glu Arg Gln Pro Thr Glu Glu Glu Tyr Leu Ala Ile Cys Leu Ile Met 50 55 60Leu Ala Arg Ser Asp Gly Ser Val Asn Gln Val Arg Ser Leu Pro Pro65 70 75 80Pro Val Pro Val Met Lys Ile His Ala Pro Ser Glu Lys Met Glu Tyr 85 90 95Lys Cys Ser Val Cys Gly Lys Gly Phe Gly Ser Tyr Gln Ala Leu Gly 100 105 110Gly His Lys Ala Ser His Arg Lys Leu Ile Ala Gly Val Ser Gly Gly 115 120 125Gly Asp Asp Gln Ser Thr Thr Ser Thr Thr Thr Asn Ala Thr Gly Thr 130 135 140Thr Ser Ser Gly Asn Gly Asn Gly Ser Gly Arg Thr His Glu Cys Ser145 150 155 160Ile Cys His Lys Cys Phe Pro Thr Gly Gln Ala Leu Gly Gly His Lys 165 170 175Arg Cys His Tyr Asp Gly Gly Asn Gly Asn Gly Asn Ala Asn Ser Ser 180 185 190Val Ser Ala Ser Val Gly Val Thr Ser Ser Glu Gly Val Gly Ser Thr 195 200 205Ile Ser His Arg Asp Phe Asp Leu Asn Ile Pro Ala Leu Pro Glu Phe 210 215 220Trp Pro Gly Phe Gly Ser Gly Glu Asp Glu Val Glu Ser Pro His Pro225 230 235 240Ala Lys Lys Ser Arg Leu Ser Leu 245194269PRTGossypium raimondii 194Leu Ala Leu Cys Phe Cys Ser Gln Thr Arg Arg Asp Ser Glu Val Phe1 5 10 15Asp Met Ala Leu Glu Ala Leu Asn Ser Pro Ala Thr Pro Phe Thr Asn 20 25 30Lys Tyr Asp Asp Val Asp Asn Asn Tyr Val Glu Thr Trp Lys Lys Gly 35 40 45Lys Arg Ser Lys Arg Gln Arg Gly Asp Ser Pro Ala Ala Val Glu Leu 50 55 60Gln Pro Thr Thr Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu65 70 75 80Ala Arg Gly Ser Ser Gly Ala Asp Leu Asp Val Ile Arg Arg Ser Ser 85 90 95Ser Ser Ser Ser Pro Pro Pro Pro Pro Pro Ala Leu Lys Leu Ser Tyr 100 105 110Lys Cys Ser Val Cys Asp Lys Ala Phe Pro Ser Tyr Gln Ala Leu Gly 115 120 125Gly His Lys Ala Ser His Arg Lys Pro Leu Ser Ala Asp Ala Ala Thr 130 135 140Thr Thr Ala Ala Ala Asn Val Asp Asn Pro Ser Thr Thr Ser Thr Ala145 150 155 160Thr Thr Ala Thr Ser Ser Gly Arg Leu His Glu Cys Ser Ile Cys His 165 170 175Lys Ser Phe Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys His 180 185 190Tyr Glu Gly Gly Asn Asn Asn Asn Asn Asn Asn Lys Asn Asn Asn Asn 195 200 205Ser Gly Ser Val Ser Val Ser Gly Val Thr Ser Ser Asp Gly Gly Ala 210 215 220Leu Ser His Asn His Arg Ala Val Asp Phe Asp Phe Asp Leu Asn Leu225 230 235 240Pro Ala Leu Pro Glu Phe Ser Gln Met Tyr Pro Asp Glu Glu Glu Val 245 250 255Gln Ser Pro Leu Pro Thr Gln Lys Pro Arg Phe Leu Ile 260 265195195PRTVitis aestivalis 195Glu Ser Trp Ala Lys Arg Lys Arg Ser Lys Arg Pro Arg Phe Asp Asn1 5 10 15Gln Pro Thr Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu Ala 20 25 30Arg Gly Gly Ala Ala Ser Ser Thr Val Ser His Arg Arg His Leu Ser 35 40 45Pro Pro Pro Ala Leu Gln Val Glu Ala Pro Lys Leu Thr Tyr Lys Cys 50 55 60Ser Val Cys Asn Lys Ala Phe Ala Ser Tyr Gln Ala Leu Gly Gly His65 70 75 80Lys Ala Ser His Arg Lys Gln Ser Gly Ser Asp Asp Leu Ser Ala Ser 85 90 95Ile Thr Thr Thr Ser Thr Ala Ala Ala Ala Ser Gly Gly Arg Thr His 100 105 110Glu Cys Ser Ile Cys His Lys Thr Phe Pro Thr Gly Gln Ala Leu Gly 115 120 125Gly His Lys Arg Cys His Tyr Glu Gly Gly Ala Ser Val Ser Ser Gly 130 135 140Val Thr Ser Ser Glu Gly Val Gly Ser Thr His Ser His Arg Asp Phe145 150 155 160Asp Leu Asn Leu Pro Ala Phe Pro Glu Leu Trp Ser Ala Arg Arg Phe 165 170 175Pro Val Asp Asp Glu Val Glu Ser Pro Leu Pro Thr Lys Lys Pro Arg 180 185 190Leu Gln Met 195196226PRTLycopersicon esculentum 196Met Ala Leu Glu Ala Leu Asn Ser Pro Thr Gly Thr Thr Ser Asn Pro1 5 10 15Gln Thr Phe Gln Phe Glu Ser Lys Gly Gln Gln Gln Leu Arg Tyr Leu 20 25 30Glu Asn Trp Thr Lys Gly Lys Arg Ser Lys Arg Ser Arg Ser Met Asp 35 40 45Arg Gln Pro Thr Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu 50 55 60Ala Arg Ser Asp Gly Ser Val Asn His Val Arg Ser Leu Pro Pro Pro65 70 75 80Val Pro Val Met Lys Ile His Glu Thr Ala Glu Lys Met Leu Tyr Arg 85 90 95Cys Ser Val Cys Gly Lys Gly Phe Gly Ser Tyr Gln Ala Leu Gly Gly 100 105 110His Lys Ala Ser His Arg Lys Leu Ile Ala Gly Gly Asp Asp Gln Ser 115 120 125Thr Thr Ser Thr Thr Thr Asn Ala Asn Gly Thr Thr Ser Ser Gly Asn 130 135 140Gly Asn Gly Asn Gly Ser Gly Thr Gly Arg Thr His Glu Cys Ser Ile145 150 155 160Cys His Lys Cys Phe Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg 165 170 175Cys His Tyr Asp Gly Gly Asn Ser Asn Gly Asn Gly Asn Ala Asn Ala 180 185 190Asn Ser Ser Ile Ser Ala Ser Val Gly Val Thr Ser Ser Glu Gly Val 195 200 205Gly Ser Thr Ile Ser His Arg Asp Phe Asp Leu Asn Ile Pro Ala Leu 210 215 220Pro Gly225197198PRTMedicago truncatula 197Trp Thr Lys Gly Lys Arg Ser Lys Arg Ser Arg Met Asp Gln Ser Ser1 5 10 15Cys Thr Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile Met Leu Ala Arg 20 25 30Ser Gly Asn Asn Asn Asp Asn Lys Thr Glu Ser Val Pro Val Pro Ala 35 40 45Pro Leu Thr Thr Val Lys Leu Ser His Lys Cys Ser Val Cys Asn Lys 50 55 60Ala Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg65 70 75 80Lys Ala Val Met Ser Ala Thr Thr Val Glu Asp Gln Thr Thr Thr Thr 85 90 95Ser Ser Ala Val Thr Thr Ser Ser Ala Ser Asn Gly Lys Asn Lys Thr 100 105 110His Glu Cys Ser Ile Cys His Lys Ser Phe Pro Thr Gly Gln Ala Leu 115 120 125Gly Gly His Lys Arg Cys His Tyr Glu Gly Ser Val Gly Ala Gly Ala 130 135 140Gly Ser Ser Ala Val Thr Ala Ala Ser Glu Gly Val Gly Ser Ser His145 150 155 160Ser His His Arg Asp Phe Asp Leu Asn Leu Pro Ala Phe Pro Asp Phe 165 170 175Ser Lys Lys Phe Phe Val Asp Asp Glu Val Ser Ser Pro Leu Pro Ala 180 185 190Ala Lys Lys Pro Cys Leu 195198219PRTPopulus sp.misc_feature(199)..(199)Xaa can be any naturally occurring amino acid 198Lys Lys Asn Ser Val Ser Arg Ile Gln Ile Met Ala Leu Glu Ala Leu1 5 10 15Asn Ser Pro Thr Thr Ala Ala Pro Leu Asn Tyr Glu Glu Thr Trp Ile 20 25 30Lys Arg Lys Arg Ser Lys Arg Pro Arg Ser Glu Ser Pro Ser Thr Glu 35 40 45Glu Glu Tyr Leu Ala Phe Cys Leu Ile Met Leu Ala Arg Gly Gly Ser 50 55 60Thr Ala Ala Thr Ala Lys Lys Thr Ala Ser Ala Ser Pro Ala Pro Pro65 70 75 80Gln Pro Pro Thr Leu Asp Leu Ser Tyr Lys Cys Thr Val Cys Asn Lys 85 90 95Ala Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg 100 105 110Lys Ser Ser Ser Glu Ser Thr Val Ala Thr Ala Ala Glu Asn Pro Ser 115 120 125Ala Ser Thr Thr Thr Asn Thr Thr Thr Thr Thr Thr Asn Gly Arg Thr 130 135 140His Glu Cys Ser Ile Cys His Lys Thr Phe Leu Thr Gly Gln Ala Leu145 150 155 160Gly Gly His Lys Arg Cys His Tyr Glu Gly Thr Ile Gly Gly Asn Asn 165 170 175Ser Ser Ser Ala Ser Ala Ala Ile Thr Thr Ser Asp Gly Gly Ala Val 180 185 190Gly Gly Gly Gly Val Ser Xaa Ser Lys Ser Gln Arg Ser Gly Gly Gly 195 200 205Phe Asp Phe Asp Leu Asn Leu Pro Ala Leu Pro 210 215199240PRTCitrus sinensismisc_feature(235)..(236)Xaa can be any naturally occurring amino acid 199Arg Tyr Glu Asp Ser Trp Thr Lys Arg Arg Arg Ser Lys Arg Leu Arg1 5 10 15Thr Asp Glu Ser Pro Gln Leu Pro Ala Ala Ala Ala Ala

Pro Thr Glu 20 25 30Glu Glu Tyr Met Ala Leu Cys Leu Ile Met Leu Ala Arg Gly Thr Thr 35 40 45Thr Ala Asn Thr Ala Pro Ala Glu Arg Thr Pro Thr Leu Ala Pro Glu 50 55 60Gln Lys Pro Leu Asp Gln Phe Pro Glu Pro Pro Ser Leu Lys Leu Ser65 70 75 80Tyr Lys Cys Ser Val Cys Asn Lys Ala Phe Ser Ser Tyr Gln Ala Leu 85 90 95Gly Gly His Lys Ala Ser His Arg Lys Asn Ala Ala Asp Ala Ser Ala 100 105 110Ser Pro Asn Ala Ala Ala Ala Ser Asp Val Thr Pro Pro Pro Ser Ala 115 120 125Thr Ala Ser Ser Gly Ser Gly Gly Arg Thr His Glu Cys Ser Ile Cys 130 135 140His Lys Ser Phe Pro Thr Gly Gln Ala Leu Gly Gly His Lys Arg Cys145 150 155 160His Tyr Glu Gly Gly Ile Asn Asn Asn Asn Asn Ser Ser Ser Asn Asn 165 170 175Asn Lys Ser Asn Asn Asn Ser Asp Val Val Thr Ser Gly Ser Ala Ser 180 185 190Val Gly Ala Ser Ala Val Thr Phe Ser Glu Gly Gly Gly Ser Ser Ser 195 200 205Gln Arg Gly Phe Asp Leu Asn Leu Pro Ala Leu Pro Glu Phe Trp Ser 210 215 220Gln Glu Val Glu Ser Pro Leu Pro Ala Lys Xaa Xaa Lys Leu Leu Met225 230 235 240200196PRTLotus corniculatus 200Trp Ala Lys Arg Lys Arg Ser Lys Arg Ser Arg Thr Asp Ser His His1 5 10 15Asn His Ala Ser Cys Thr Glu Glu Glu Tyr Leu Ala Leu Cys Leu Ile 20 25 30Met Leu Ala Arg Gly Ser Thr Ala Val Thr Pro Lys Leu Thr Leu Ser 35 40 45Arg Pro Ala Pro Val Thr Ala Glu Lys Leu Ser Tyr Lys Cys Ser Val 50 55 60Cys Glu Lys Thr Phe Pro Ser Tyr Gln Ala Leu Gly Gly His Lys Ala65 70 75 80Ser His Arg Lys Leu Ala Gly Ala Ala Ala Glu Asp His Ser Thr Ser 85 90 95Ser Ala Val Thr Thr Ser Ser Ala Ser Asn Gly Gly Gly Lys Val His 100 105 110Gln Cys Ser Ile Cys Gln Lys Ser Phe Pro Thr Gly Gln Ala Leu Gly 115 120 125Gly His Lys Arg Cys His Tyr Glu Gly Gly Gly Gly Ala Ser Ser Thr 130 135 140Ala Thr Ala Thr Ala Ser Glu Gly Val Gly Ser Thr His Ser His Gln145 150 155 160Arg Asn Phe Asp Leu Asn Leu Pro Ala Phe Pro Asp Phe Ser Ala Ser 165 170 175Lys Phe Phe Val Glu Glu Glu Val Ser Ser Pro Leu Pro Ser Lys Lys 180 185 190Pro Arg Leu Leu 195201687DNALolium perenne 201atggccgtgg aggcggttct cgaagcggcg gcgatgatac agtcgccgcc gagcaagaag 60atggaggcgt ctagtagcag cgacgaggcg ttcgaggcgt tgcagcagca cacggagggg 120tggtccaaga agaagcgctc gaggcggcca cgggcgctcg agcccagcga ggaggagtac 180ctcgcgttct gcctcgtcat gctggcgcgc ggccaccgcg acgccgcgcc ggagcacggg 240tgctccgtct gcggcaaggc gttcgcgtcg taccaggcgc tcggcggcca caaggccagc 300caccggaagc cacccacagc tccagccgcg gtggcagcaa gcgccgtccc cgaggaggac 360aagccacggg cggctgcctc gtcctcgtct gggtccggcg atgccgctgg cggcggcaag 420gtccacgagt gcaacgtgtg ccagaagacg ttcccgacgg ggcaggcgct gggcggccac 480aagcggtgcc actacgacgg caccatcggc agcgccgccg cgcccacggt gaaggctgcc 540aaggccgccg ccgcggcgag cgcgccgacg gcgacgaacc gggggttcga cctgaacgtg 600ccggcgctgc cgggactcgc ggaggagggg gaggaggtgc tcagcccggt atccttcaag 660aagccgaggc tcatgatcac cgcgtga 687202753DNAOryza sativa 202atggcggtgg aggcggttct tgaggcgtcg agaagtagta gtgaggagga ggcggaggtg 60atcgtcacgc acggcggcgg cggcggcggc ggaggaggag gaggacaggt ggaggggtgg 120gggaagcgga agcggtcgcg gcggcggcga ccgcagctgc cgccctccga ggaggagtac 180ctcgcgctct gcctcctcat gctggcgcgc gggcgacgcg acggcgacga cgtggcggcg 240tcggcgtcgg cggcggcggc ggcggtggag caccggtgct ccgtctgcgg caaggcgttc 300gcgtcctacc aggctctcgg cggccacaag gccagccacc ggaagccgcc gccgccgccg 360ccgcccgcca tggtcgacga cgacgaggtg gtggtggaga cgaaaccggc ggctatcgcg 420acgccgtcct cgtcggcgtc gggcgtctcc ggcggcggcg gcgggagggc gcacgagtgc 480aacgtgtgcg gcaaggcgtt cccgacgggg caggcgctgg gcggccacaa gcgatgccac 540tacgacggca cgatcggcag cgccgccggc gccggcgcgt ccaagccggc ggcgaagacg 600accgtggcgg tggcggcgag ccggggcttc gacctcaacc tgccggcgct gccggacgtc 660gccgccgccg ccgaccagcg gtgcgcggcg gaggacgacg aggtgctcag ccctctcgcc 720ttcaagaagc ccaggctcat gatcccggca tag 753203717DNAOryza sativa 203atggcggtgg aggaggttct tgatggcgcc gcgccgatgc tgtcgtcgtc gccggcggcg 60agcggcgagg aggtgggggc gcggaagccg cagcaacggt gcggcggcgc cgaggggtgg 120tccaagcgga agcgctcgag gcggcgccac cgcgaccgcg ccgctgcgcc gccgcctcac 180ggttcggagg aggagcacct cgcgctcagc ctcctcatgc tggcgcgcgg ccaccgcgac 240ccctcgccgg cgccgcagga gcagcacggg tgctccgtgt gcggcagggt gttctcgtcc 300taccaggcgc tcggcggcca caagacgagc caccgcccca ggacgccgcc gacgatggcg 360gccgttgtcg tcgtagacga gccggcagcg acgacggcct cgccggccgc gtcctcgtcc 420aattccggct ccggcagcgg cggcggcggc ggaaacaagg tgcacgagtg ctccgtgtgc 480aagaagacgt tcccgacggg gcaggcgctg ggcgggcaca agcggtgcca ctacgagggc 540ccgatcggaa gcggcggcgg cgccgctgtc gccggccgcg ggttcgatct gaacctgccg 600gcggtggcgc tgccggacat catgacggag cggtgcttgc cggcggcggc cgaggaggag 660gaggtgctca gcccgcttgc aagcttcaag aagccaaggc taatgatccc tgcttaa 717204786DNATriticum aestivum 204atgtcgtcgt cggccatgga agcgctccac gccctgatcc cggagcagca ccagctggac 60gttgaggcgg ctgcggctgt cagcagcgcc accagcggcg aggagagcgg ccacgtgctg 120caggggtggg ccaagaggaa gcgatcgcgc cgccagcgct ccgaggagga gaacctcgcg 180ctctgcctcc tcatgctctc gcgcggcggc aagcagcgtg ttcaggcgcc gcagccggag 240tcgttcgctg cgccggtgcc tgccgagttc aagtgctccg tctgcggcaa gtccttcagc 300tcctaccagg cgctcggagg ccacaagacg agccaccggg tgaagcagcc gtctcctccc 360tctgatgccg ctgctgcccc actcgtggcc ctcccggccg tcgccgccat cctgccgtcc 420gccgagccgg ccacgtcgtc caccgccgcg tcctccgacg gcgcgaccaa cagagtccac 480aggtgctcca tctgccaaaa ggagttcccg actgggcagg cgctcggcgg gcacaagagg 540aagcactacg acggaggcgt gggcgccgcc gcctcgtcga ccgagcttct ggccgccgcg 600gccgccgagt ctgaggtggg gagcaccggc aacgggagct ccgccgcccg ggccttcgac 660ctgaacattc cggccgtgcc ggagttcgtg tggaggccgt gcgccaaggg caagatgatg 720tgggaggacg atgaggaggt gcagagcccc ctcgccttca agaagcctcg gcttctcacc 780gcttga 786205810DNAOryza sativa 205atgtcgagcg cgtcgtccat ggaagcgctc cacgccgcgg tgctcaagga ggagcagcag 60cagcacgagg tggaggaggc gacggtcgtg acgagcagca gcgccacgag cggggaggag 120ggcggacacc tgccccaggg gtgggcgaag cggaagcggt cgcgccgcca gcgatcggag 180gaggagaacc tcgcgctctg cctcctcatg ctcgcccgcg gcggccacca ccgcgtccag 240gcgccgcctc cgctctcggc ttcggcgccc ccgccggcag gtgcggagtt caagtgctcc 300gtctgcggca agtccttcag ctcctaccag gcgctcggcg gccacaagac gagccaccgg 360gtcaagctgc cgactccgcc cgcagctccc gtcttggctc ccgcccccgt cgccgccttg 420ctgccttccg ccgaggaccg cgagccagcc acgtcatcca ccgccgcgtc ctccgacggc 480atgaccaaca gagtccacag gtgttccatc tgccagaagg agttccccac cgggcaggcg 540ctcggcgggc acaagaggaa gcactacgac ggtggcgtag gcgccggcgc cggcgcatct 600tcaaccgagc tcctggccac ggtggccgcc gagtccgagg tgggaagctc cggcaacggc 660cagtccgcca cccgggcgtt cgacctcaac ctcccggccg tgccggagtt cgtgtggcgg 720ccgtgctcca agggcaagaa gatgtgggac gaggaggagg aggtccagag ccccctcgcc 780ttcaagaagc cccggcttct caccgcgtaa 810206663DNAOryza sativa 206atggcgctcg acgggaagcc accggtgccg ccgccgtcca cgccgccgat ggactcgtgg 60gcctgcggtg gtcgccgctc caagcgccgc ggcggcggcg gcgggagcag cgggagtagt 120ggcagctccg gcggcggcgg cggcggcgag tccgaggagg agtacctcgc ggcctgcctc 180ttgatgctcg cgcatggcgt ccgcgacgag gccgaggtcg tcggcgtcgc ggcggcgacg 240gcgaagccac agcatgggta cgagtgctcg gtgtgcggca aggtgtacgg gtcctaccag 300gcgctgggcg ggcacaagac gagccaccgc aagccgccgt cgccggcggc cgaaccggcg 360gccggcgaag agccgtcctc cggcggggtg gccggcgagg cgaaggtgca ccgttgctcc 420atctgcctcc gcacgttccc gtccgggcag gcgctgggcg ggcacaagcg gctgcactac 480gagggcggcg ccgtcggaga cgccgtcaag gagaagaact ccctgaagac caaggcggcg 540gtggcgacag cggtgctgaa ggacttcgac ctgaacctgc cggccgcggc gacgacggcg 600ggggacgagg ccgagagctc accaccggag gccaagagag cacgtctgct gcttctcgtc 660taa 663207737DNAAegilops speltoides 207tcggcacgag gccctgctcc atcaccacta gccagtagct agctactccc tcgcctcact 60tccttcccga accactagct aactactgcc caccactcct ccttcgtgcc agagagaata 120agcaacacgt tcttgcgtgt gttggatcgg cgtacgtgcg tgcatggcgg tggacgcggt 180tcgcgacgcg gcggcgatgg tgagcgagga ggaggaggag gggcagctgc ggtgcgacga 240ggggtggggc aagcggaggc gcccgaggcg ccagcggcag cgcgcgccca gcgaggagga 300gcacctcgcg ctcagcctcc tcatgctggc gcgcggtcac cgcgaccggc acctgcttgg 360gtcgtccgag ccggcgcagg agcaccgctg ctccgtgtgc ggcaaggggt tcccgtccta 420ccaggccctg ggcgggcaca aggcgagcca ccgcccgaag ccggcgcccg ccggcgcgga 480cgagcccgct gccacgacgg cggcctcgcc cgccgcatcc tcgtcaacga cgtccagcgg 540cgcgggcgga ggtggcaggg tgcacgagtg ctccgtgtgc aagaagacct tcccgaccgg 600gcaggcgctg ggcgggcaca agcggtgcca ctacgagggc cccatcggcg ccaccgtggt 660tgcgagccga gggttcgacc tgaacctgcc ggcgctgccg gacatcgtca ccgagcgcga 720gcggtgcatg ccggcac 737208655DNASecale cereale 208gcggaggcgc ccgaggcggc agcggcagcg cgcgcccagc gaggaggagc acctcgcgct 60cagcctcctc atgctggcgc gcggtcaccg cgaccggcac ctgcctccgt cgtccgagcc 120ggcacaggag caccgctgct ccgtgtgcgg caaggggttc ccgtcctacc aggccctcgg 180cgggcacaag gcgagccacc gcccgaagcc ggcgcccgcc ggcgcggacg agcccgctgc 240cacggcggcg gcctcgcccg ccgcatcctc gtcaacgacg tccagcggcg cgggtgtcaa 300ggtgcacgag tgctccgtgt gcaagaagac cttcccgacg gggcaggcgc tgggcgggca 360caagcggtgc cactacgagg gccccatcgg cggcggcggt gcccccgcgg ttgcgagccg 420agggttcgac ctgaaccttc cggcgctgcc ggacatcgtc accgagcgcg agcggtgcat 480gccggcgccg gccgatgagg aggaggtgct cagcccgctg gcgttcaaga agccgaggct 540aatgatcccg gcataattaa atcatcaaag aattaatgag tgaattgcgt cggtgtgtat 600tctttttgga ttgcttatgc tcggctggtt ggtgtaaaaa aaaaaaaaaa aaaaa 655209666DNASaccharum officinarummisc_feature(615)..(615)n is a, c, g, or t 209ggacgacgtg gtggcggctg cagcggatca ggtcgccacg accagcaaca gcagcggcac 60ggcggcagag gaggacaagg atgtcaagac ggcggtgcag caggagcatg gccaagggct 120ggcgaagcgg aagcgctcca ggcgccgccg cgaccgcgag cagcagcagc tgcccaagga 180gcaccccacc caggaggagt acctggcgca gtgcctcgtc atgctggcca ccggccgccg 240cgacggcgac gtcccggccc tggcctccgc gccgccgccg ccgcaggggc agcagcagga 300ccacgcgtgc tccgtctgcg gcaaggcgtt cccgacgtac caggcgctcg gcgggcacaa 360ggccagccac cgcaccaggc cctcgccgcc gtcggcggca acggaagtag taggggatca 420ccacgaggag cagaagccgg tgctgccgtc ctcgtcgtcc gcggcctccg ctggcgccga 480caacaacaag cccgcggcgg cgcacgagtg caacgtgtgc ggcaaggcgt tcccgacggg 540gcaggcgctg ggcggccaca agcgccgcca ctacgacggc accatcggca gcgccgccgc 600gcccgcgcgc gcgtnctnct nctnctncgc gggcggcggc caacagcagc cgcgccacgc 660cggcgn 666210762DNANicotiana benthamiana 210atggcacttg aagctttgaa ttctccaact acaacaactc caccaacttt ccaatttgag 60aacaacggcc cgcttcgata ccttgaaaat tggactaaag gtaaaagatc aaaaaggcct 120cgtagtatgg aacgacagcc tactgaagaa gagtatttgg ctctttgttt gattatgcta 180gcgcgtagcg atggctctgc taatcgggaa cagtctctac caccaccgcc agttccagtg 240atgaaaatac acgcgccacc ggaggagaag atggtgtaca agtgttcagt ttgtggtaag 300ggttttggat cttatcaagc tttaggagga cacaaggcta gtcaccggaa gctcgtcgcc 360ggcggtggag gaggagatga ccagtcaact acctccacaa ccactaacgc tacaggaact 420actagctctg ctaacggtaa cggtaacgga agcggaaaaa ctcacgagtg ttcaatttgc 480cacaagcgtt ttcctactgg acaggctttg ggtggacaca aaaggtgtca ctacgacggc 540ggtaacagta acggtggcgt tagcgttagt gctagcgttg gactgacgtc atcagaaggt 600gtggggtcca ctgtgagtca ccgtgacttt gacttgaaca ttccggcgtt gccggaattc 660tggcccggat ttggttccgg cgaggatgaa gtggagagtc ctcatccgac gaagaaatcg 720cggctatctc tgcctccaaa atttgaatta ttccgggaat ag 762211786DNACapsicum annuum 211atggcacttg aagctttgaa ttctccaact ggtacaccaa ctccgccacc gtttcaattt 60gagagcgacg gccaacagct tcgatatatc gaaaactgga ggaagggaaa gagatctaaa 120aggtcacgca gcatggagca ccagcctact gaggaagaat acttagcgct ttgtttgatc 180atgcttgcac gtagcggtgg ctccgttaat catcaacgat ctctaccacc gccggctccg 240gtgatgaaac tgcacgcgcc gtcgtcatca tcggcggcgg aggaggagaa ggagaagatg 300gtgtataagt gttcggtttg tggtaaggga tttgggtctt atcaagcttt aggtggacac 360aaagctagtc accggaaact cgtacccggc ggagatgatc agtcaactac ctccacaacc 420actaacgcaa ccggaacaac aacctccgtt aacggcaacg gcaacagaag tggaaggact 480cacgagtgtt cgatttgtca caagtgtttt cccactggac aagctttagg tggacacaaa 540aggtgtcact acgacggcgg tatcggtaac ggaaacgcta acagtggcgt tagtgctagc 600gttggagtga cgtcatcgga gggtgtgggg tccacagtca gtcaccggga tttcgacttg 660aacattccgg cgttgccgga attctggctg ggatttggtt ccggcgaaga tgaggtggag 720agtccacatc cggcgaagaa atcgcggtta tgtttgcctc caaaatatga attatttcaa 780cattaa 786212708DNAMedicago sativa 212atggctatgg aagcacttaa ctcacccacc actgctactc ctttcacacc ctttgaggaa 60ccaaatctga gttatcttga aacaccgtgg acgaaaggta aacgatcaaa gcgttctcgc 120atggatcaat cttcatgcac tgaagaagag tatctcgctc tttgtctcat catgcttgct 180cgcagcggta acaacaacga caaaaagtct gattcggtgg cgacgccgct aaccaccgtt 240aaactcagtc acaaatgctc agtctgcaac aaagctttct catcttatca agccctaggt 300ggacacaaag ccagtcaccg gaaagctgtt atgtccgcaa ccaccgctga agatcagatc 360accaccactt catccgccgt gactaccagc tctgcttcca acggtaagaa caagactcat 420gagtgttcca tctgtcacaa atccttccct actggacagg ctttgggagg acacaagcgt 480tgtcactacg aaggcagcgt tggtgccggt gccggtgctg gaagtaacgc tgtaactgcc 540tctgaaggag ttggattgtc acacagccac caccgtgatt ttgatcttaa cctcccggct 600tttccggact tttcaaagaa gtttttcgtg gatgacgagg tttttagtcc tttacctgct 660gcaaagaagc cctgtctttt caagctggaa attccttctc attactga 708213723DNAArtificialGlycine max 213atggctttgg aagctctcaa ctcaccaaca acaaccgctc catcttttcc ctttgacgac 60ccaactattc catgggcgaa acgaaaacgt tcaaagcgtt ctcgcgacca tccttctgaa 120gaagagtacc tcgccctctg cctcatcatg ctcgctcgcg gcggcaccac caccgtcaac 180aaccgccacg tcagccctcc gccgctacag ccacagccac agccgacacc agatccttcc 240accaagctca gttacaaatg ctccgtttgc gacaagagct tcccctctta ccaagcgctc 300ggtggacaca aggccagtca ccggaaactc gccggcgccg ccgaagacca accccccagc 360accaccactt cctccgccgc cgccaccagc tccgcctccg gaggtaaggc ccatgagtgc 420tccatttgcc acaaatcctt ccccaccgga caggcccttg gcggacacaa acgttgtcac 480tacgaaggta acggtaacgg aaataacaac aacagtaaca gcgttgtcac cgtcgcctcg 540gaaggcgtgg gctccaccca cactgtcagt cacggccacc accgcgactt cgatctcaac 600atcccggcct ttccggattt ttcgaccaag gtcggagaag acgaggttga gagccctcac 660cctgtcatga agaagcctcg cctcttcgtc attcccaaga tcgaaatccc ccaatttcaa 720tga 723214798DNAArabidopsis thaliana 214atggttgcat acccaaccaa aaaaacagca attaaacaat ttcttcactg caattcacaa 60gcaaccttca aactaaaact cgagagacaa gaaatcctca gaatctttaa cttaatggcg 120ctcgaggctc ttacatcacc aagattagct tctccgattc ctcctttgtt cgaagattct 180tcagtcttcc atggagtcga gcactggaca aagggtaagc gatctaagag atcaagatcc 240gatttccacc accaaaacct cactgaggaa gagtatctag ctttttgcct catgcttctc 300gctcgcgaca accgtcagcc tcctcctcct ccggcggtgg agaagttgag ctacaagtgt 360agcgtctgcg acaagacgtt ctcttcttac caagctctcg gtggtcacaa ggcaagccac 420cgtaagaact tatcacagac tctctccggc ggaggagatg atcattcaac ctcgtcggcg 480acaaccacat ccgccgtgac tactggaagt gggaaatcac acgtttgcac catctgtaac 540aagtcttttc cttccggtca agctctcggc ggacacaagc ggtgccacta cgaaggaaac 600aacaacatca acactagtag cgtgtccaac tccgaaggtg cggggtccac tagccacgtt 660agcagtagcc accgtgggtt tgacctcaac atccctccga tccctgaatt ctcgatggtc 720aacggagacg acgaagtcat gagccctatg ccggcgaaga agcctcggtt tgactttccg 780gtcaaacttc aactttaa 798215684DNAArabidopsis thaliana 215atggcgctcg aggctcttac atcaccaaga ttagcttctc cgattcctcc tttgttcgaa 60gattcttcag tcttccatgg agtcgagcac tggacaaagg gtaagcgatc taagagatca 120agatccgatt tccaccacca aaacctcact gaggaagagt atctagcttt ttgcctcatg 180cttctcgctc gcgacaaccg tcagcctcct cctcctccgg cggtggagaa gttgagctac 240aagtgtagcg tctgcgacaa gacgttctct tcttaccaag ctctcggtgg tcacaaggca 300agccaccgta agaacttatc acagactctc tccggcggag gagatgatca ttcaacctcg 360tcggcgacaa ccacatccgc cgtgactact ggaagtggga aatcacacgt ttgcaccatc 420tgtaacaagt cttttccttc cggtcaagct ctcggcggac acaagcggtg ccactacgaa 480ggaaacaaca acatcaacac tagtagcgtg tccaactccg aaggtgcggg gtccactagc 540cacgttagca gtagccaccg tgggtttgac ctcaacatcc ctccgatccc tgaattctcg 600atggtcaacg gagacgacga agtcatgagc cctatgccgg cgaagaagcc tcggtttgac 660tttccggtca aacttcaact ttaa 684216744DNADatisca glomerata 216atggctctag aagcgctcaa ctctccgacc acagctacgc cggtgtttca ctacgacgac 60cccagcttga attaccttga gccatggacc aagcgtaagc gttccaagcg tacgcgctta 120gatagccctc ataccgagga agagtacctt gctttctgcc tcatcatgct cgctcgtggc 180cgcgttgcct ctgcaaatcg acgggattct cagtcttcca ttcagattca gcctgaagca 240acgacttcgg ctaccaaagt cagttataag tgctctgtgt gcgataaggc cttttcgtct 300tatcaggctt tgggtgggca caaggccagc cacagaaagc tcgctggcgg cgaagatcaa 360tcgacttcct ttgccaccac gaattcagcc accgtcacta ccaccacagc ctccggaggt 420ggtggcaggt ctcatgagtg ttctatttgc cacaaatcgt tcccgactgg ccaggccttg 480ggtggtcaca agcgctgcca ctacgaaggc agtatcggcg gcaatagtat tcaccaccac 540aacaatacca ccaacagcgg aagcaacggt

ggcatgagca tgacctccga agtaggttcc 600acacacacag tcagccacag tcaccgtgac ttcgatctca acatcccggc cttgccggag 660tttcggtcga atttcttcat atccggggat gacgaggtcg agagtcctca tccggccaag 720aaaccccgta tattgatgaa ataa 744217684DNAArabidopsis thaliana 217atggcgctcg aggctcttac atcaccaaga ttagcttctc cgattcctcc tttgttcgaa 60gattcttcag tcttccatgg agtcgagcac tggacaaagg gtaagcgatc taagagatca 120agatccgatt tccaccacca aaacctcact gaggaagagt atctagcttt ttggctcatg 180cttctcgctc gcgacaaccg tcagcctcct cctcctccgg cggtggagaa gttgagctac 240aagtgtagcg tctgcgacaa gacgttctct tcttaccaag ctctcggtgg tcacaaggca 300agccaccgta agaacttatc acagactctc tccggcggag gagatgatca ttcaacctcg 360tcggcgacaa ccacatccgc cgtgactact ggaagtggga aatcacacgt ttgcaccatc 420tgtaacaagt cttttccttc cggtcaagct ctcggcggac acaagcggtg ccactacgaa 480ggaaacaaca acatcaacac tagtagcgtg tccaactccg aaggtgcggg gtccactagc 540cacgttagca gtagccaccg tgggtttgac ctcaacatcc ctccgatccc tgaattctcg 600atggtcaacg gagacgacga agtcatgagc cctatgccgg cgaagaagcc tcggtttgac 660tttccggtca aacttcaact ttaa 684218762DNAPetunia x hybrida 218atggcacttg aagcattgaa ttctccaact acaacaacac caccatcatt ccaatttgag 60aacaacgggc ttaagtacct tgagagttgg acaaaaggta aaagatcaaa aaggcaacgc 120agcatggaac gacagtgtac tgaagaagag tatttagcac tttgtcttat catgctagca 180cgtagcgatg gttctgttaa taactcacgg tctctaccac caccaccact accaccatca 240gttccagtaa cgtcgcaaat aaacgcgacg ttattggaac agaagaattt gtacaagtgt 300tccgtttgtg gtaaagggtt tgggtcttat caagctttag gtggacataa agcaagtcac 360cggaaacttg tcagcatggg aggagatgaa caatctacta cttccactac tactaacgta 420acgggaacta gttccgctaa cgttaacggt aacggaagaa ctcacgaatg ttcaatttgt 480cacaagtgct ttcctactgg acaagcttta ggtggtcata aaaggtgcca ctatgacggt 540ggtaacggta acggtaacgg aagtgtaagt gttggggtga cgtcatctga aggtgtgggg 600tccactatta gtcatcaccg tgactttgac ttgaatattc ccgcgttgcc ggagttttgg 660ccgggatttg gttccggcga ggatgaggtg gagagtcctc atccagcaaa gaagtcaagg 720ctatctcttc cacctaaact tgaattattc aaaggattat ag 762219822DNAArabidopsis thaliana 219atggccctcg aagcgatgaa cactccaact tcttctttca ccagaatcga aacgaaagaa 60gatttgatga acgacgccgt tttcattgag ccgtggctta aacgcaaacg ctccaaacgt 120cagcgttctc acagcccttc ttcgtcttct tcctcaccgc ctcgatctcg acccaaatcc 180cagaatcaag atcttacgga agaagagtat ctcgctcttt gtctcctcat gctcgctaaa 240gatcaaccgt cgcaaacgcg atttcatcaa cagtcgcaat cgttaacgcc gccgccagaa 300tcaaagaacc ttccgtacaa gtgtaacgtc tgtgaaaaag cgtttccttc ctatcaggct 360ttaggcggtc acaaagcaag tcaccgaatc aaaccaccaa ccgtaatctc aacaaccgcc 420gatgattcaa cagctccgac catctccatc gtcgccggag aaaaacatcc gattgctgcc 480tccggaaaga tccacgagtg ttcaatctgt cataaagtgt ttccgacggg tcaagcttta 540ggcggtcaca aacgttgtca ctacgaaggc aacctcggcg gcggaggagg aggaggaagc 600aaatcaatca gtcacagtgg aagcgtgtcg agcacggtat cggaagaaag gagccaccgt 660ggattcatcg atctaaacct accggcgtta cctgaactca gccttcatca caatccaatc 720gtcgacgaag agatcttgag tccgttgacc ggtaaaaaac cgcttttgtt gaccgatcac 780gaccaagtca tcaagaaaga agatttatct ttaaaaatct aa 822220717DNAArabidopsis thaliana 220atggcacttg aaactcttac ttctccaaga ttatcttctc cgatgccgac tctgtttcaa 60gattcagcac tagggtttca tggaagcaaa ggcaaacgat ctaagcgatc aagatctgaa 120ttcgaccgtc agagtctcac ggaggatgaa tatatcgctt tatgtctcat gcttcttgct 180cgcgacggag atagaaaccg tgaccttgac ctgccttctt cttcgtcttc acctcctctg 240cttcctcctc ttcctactcc gatctacaag tgtagcgtct gtgacaaggc gttttcgtct 300taccaggctc ttggtggaca caaggcaagt caccggaaaa gcttttcgct tactcaatct 360gccggaggag atgagctgtc gacatcgtcg gcgataacca cgtctggtat atccggtggc 420gggggaggaa gtgtgaagtc gcacgtttgc tctatctgtc ataaatcgtt cgccaccggt 480caagctctcg gcggccacaa acggtgccac tacgaaggaa agaacggagg cggtgtgagt 540agtagcgtgt cgaattctga agatgtgggg tctacaagcc acgtcagcag tggccaccgt 600gggtttgacc tcaacatacc gccgataccg gaattctcga tggtcaacgg agacgaagag 660gtgatgagtc ctatgccggc gaagaaactc cggtttgact tcccggggaa accctaa 717221717DNAArabidopsis thaliana 221atggcacttg aaactcttac ttctccaaga ttatcttctc cgatgccgac tctgtttcaa 60gattcagcac tagggtttca tggaagcaaa ggcaaacgat ctaagcgatc aagatctgaa 120ttcgaccgtc agagtctcac ggaggatgaa tatatcgctt tatgtctcat gcttcttgct 180cgcgacggag atagaaaccg tgaccttgac ctgccttctt cttcgtcttc acctcctctg 240cttcctcctc ttcctactcc gatctacaag tgtagcgtct gtgacaaggc gttttcgtct 300taccaggctc ttggtggaca caaggcaagt caccggaaaa gcttttcgct tactcaatct 360gccggaggag atgagctgtc gacatcgtcg gcgataacca cgtctggtat atccggtggc 420gggggaggaa gtgtgaagtc gcacgtttgc tctatctgtc ataaatcgtt cgccaccggt 480caagctctcg gcggccacaa acggtgccac tacgaaggaa agaacggagg cggtgtgagt 540agtagcgtgt cgaattctga agatgtgggg tctacaagcc acgtcagcag tggccaccgt 600gggtttgacc tcaacatacc gccgataccg gaattctcga tggtcaacgg agacgaagag 660gtgatgagtc ctatgccggc gaagaaactc cggtttgact tcccggagaa accctaa 717222708DNABrassica rapa 222atggctcttg agactctcaa ttctccgact tcagccaccg cctccgctcg gcctcttctc 60cggtatcgcg aagaaatgga gccggagaat ctcgagcaat gggctaaaag aaaacgcacc 120aaacgacaac gttttgatca gagtcgtctg aatcaagaaa cggctccttc agaagaagag 180tatctcgctc tttgtctcct catgctcgct cgtggctccg ccgtgcaatc tcctctccct 240ccgtcttcgt cctccgacca ccgtggttac aagtgtacgg tctgcggaaa gtcgttttcc 300tcttaccaag ccttaggtgg acacaagacg agtcaccgga aaccggcgag caacgttaac 360gttcccatca accaagagca gtctaataac agtcatagta acagcaacgg tggttccgtc 420gttatcaacg gtaacggcgt tagtcaaagc gggaagattc atacttgctc gatatgtttc 480aagtcgtttt cgtcaggtca ggctttgggt ggacacaaac ggtgtcacta tgacgctggt 540aataacggaa acggtaacgg cagtagcagc aacagcgtgg aggtcgtcgg tggcagtgac 600ggcagctatg tggatgatga aagatcgtca gaacagagcg cgaccggcga caaccggggg 660tttgacttga atttaccggc tgatcaagtc gcagttgtga tatcttaa 708223714DNABrassica rapa 223atggctcttg agactctcaa ttctccgact tcagccaccg cctccgctcg gcctcttctc 60cggtatcgcg aagaaatgga gccggagaat ctcgagcaat gggctaaaag aaaacgcacc 120aaacgacaac gttttgatca gagtcgtctg aatcaagaaa cggctccttc agaagaagag 180tatctcgctc tttgtctcct catgctcgct cgtggctccg ccgtgcaatc tcctctccct 240ccgtcttcgt cctccgacca ccgtggttac aagtgtacgg tctgcggaaa gtcgttttcc 300tcttaccaag ccttaggtgg acacaagacg agtcaccgga aaccggcgag caacgttaac 360gttcccatca accaagagca gtctaataac agtcatagta acagcaacgg tggttccgtc 420gctatcaacg gtaacggcgt tagtcaaagc gggaagattc atacttgctc gatatgtttc 480aagtcgtttt cgtcaggtca ggctttgggt ggacacaaac ggtgtcacta tgacgctggt 540attaacggaa acggtaacgg cagtagcagc aacagcgtgg aggtcgtcgg tggcagtgac 600ggcaactatg tggatgatga aagatcgtca gaacagagcg cgaccggcga caaccggggg 660tttgacttga atttaccggc tgatcaagtc gcagttgtga tatctaaacg ttga 714224822DNANicotiana tabacum 224atgactcttg aagctttgaa gtcacctacg gcggcaacgc cgactctacc accacgctat 60gaagatgatg atgaaattca taatttggat tcttgggcta aaggaaaacg atcaaaacgg 120ccccgtattg atgccccacc gactgaagaa gagtatttag ccctctgtct catcatgctc 180gctcgcagcg gaaccggaac cagaaccggt ttaactgatg ctactacttc ccaacaacct 240gccgataaaa aaaccgccga gttgccgccg gttcataaga aagaggtggc aacagagcaa 300gcagagcaat cttacaagtg tagcgtgtgt gacaaggctt tttcttctta tcaagcactc 360ggtgggcata aagcaagtca ccgtaaaact actactactg ctaccgccgc ctctgatgat 420aacaatcctt caacttcaac ttccactggc gccgttaata tctctgctct taatccaact 480ggtcgttcac acgtctgttc tatttgccac aaggcttttc ctactggcca agctttgggt 540gggcacaagc gccgccacta tgaaggcaaa ctcggtggta acagccgcga cttaggcggc 600ggcggcggcg gcggtcatag tggaagcgtc ttgactactt cagacggcgg cgcgtcgact 660cacacgctac gtgactttga cctgaacatg cctgcttcgc cggaattgca actgggtctg 720agtattgatt gtggacggaa aagtcaactg ttgccgatgg tccaagaggt ggaaagtcct 780atgcctgcaa agaaaccgcg tttattgttt tcgttgggtt ga 822225825DNAPetunia x hybrida 225atggctcttg aagctttaaa gtcacccaca gcagcaacac catctctacc accacgttat 60gaagatcatg ttgatatgaa taatttggat tcttgggtta aaggaaaacg atccaaacga 120cctcgaattg aaaccccacc ttctgaagaa gaatatttag cactttgtct tattatgctt 180gcccgtagcg gtaacggaac tacacccggt tcaactgata ctactattac tactactatt 240tctaaagaac cggagaagaa aaaccgtgag ctgacaccgg ttcatcaaga aacagaacaa 300tcttacaagt gtagcgtgtg tgacaagtct tttagttctt atcaagctct tggtggacac 360aaagcaagtc ataggaaaat tacaactatt gccaccaccg ccttattaga tgacaacaac 420aataatccta caacgtcaaa ttctacaagt ggcaacgttg ttaataatat ttctgcttta 480aacccaagtg gacgttcaca cgtatgttct atatgtcaca aggcttttcc aactggacaa 540gctttaggtg gacacaaacg ccgccactat gaaggcaaac taggtggtaa caacaacaac 600caccgtgacg gcggtggtca tagtggaagt gtcgtgacaa cttctgatgg tggcgcgtct 660actcacacgc tccgtgactt tgacttgaac atgttgcctc cttccccaga attgcaattg 720gggttgagta ttgactgtga tttgaaaagt caaataccaa ttgaacaaga agttgaaagt 780cctatgcctt tgaagaaacc gcgtttattg ttttctatgg attga 825226582DNAArabidopsis thaliana 226atggcgcttg aagctcttaa ttcaccaaga ttggtcgagg atcccttaag attcaatggc 60gttgagcagt ggaccaaatg taagaaacga tccaaacgtt cgagatctga tcttcatcat 120aaccaccgtc tcactgagga agagtatcta gctttctgtc tcatgcttct tgctcgggat 180ggcggcgatc ttgactctgt gacggttgcg gagaagccga gttataagtg tggcgtttgt 240tacaagacgt tttcgtctta ccaagctctc ggcggtcata aagcgagcca ccggagctta 300tacggtggtg gagagaatga taaatcgaca ccatccaccg ccgtgaaatc tcacgtttgt 360tcggtttgcg ggaaatcttt cgccaccggt caagctctcg gcggccacaa gcggtgccac 420tacgatggtg gcgtttcgaa ctcggaaggt gtggggtcta ctagccacgt cagcagtagt 480agccaccgtg gatttgacct taatattata ccggtgcagg gattttcgcc ggacgacgaa 540gtgatgagtc cgatggcgac taagaagcct cgcctgaagt aa 582227582DNAArabidopsis thaliana 227atggctcttg aagctcttaa ttcaccaaga ttggtcgagg atcccttaag attcaatggc 60gttgagcagt ggaccaaatg taagaaacga tccaaacgtt cgagatctga tcttcatcat 120aaccaccgtc tcactgagga agagtatcta gctttctgtc tcatgcttct tgctcgtgat 180ggcggcgatc ttgattctgt gacggttgag gagaagccga gttataagtg tggcgtttgt 240tacaagacgt tttcgtctta ccaagctctc ggtggtcaca aagcgagtca ccggagttta 300tacggtggtg gagataacga taagtcgaca ccatccaccg ccgtgaaatc tcacgtttgt 360tcggtttgcg ggaaatcttt cgccaccggt caagctctcg gcggccacaa gcggtgccac 420tatgatggtg gcgtttcgaa ctcggaaggt gtggggtcta ctagccacgt tagtagtagt 480agccaccgtg gatttgacct taatatttta ccggtgcagg gattctcgcg ggacgacgaa 540gtgatgagtc cgatggcgac taagaagcct cgcctgaagt aa 582228648DNAArabidopsis thaliana 228atggctctcg acactctcaa ttctcccacc tccaccacca caaccaccgc tcctcctcct 60ttcctccgtt gcctcgacga aaccgagccc gaaaacctcg aatcatggac caaaagaaaa 120cgtacaaaac gtcaccgtat agatcaacca aaccctcctc cttctgaaga agagtatctc 180gctctttgcc tccttatgct cgctcgtggc tcctccgatc atcactctcc accgtcggat 240catcactctc tttctccact gtccgatcat cagaaagatt acaagtgttc cgtctgtggc 300aaatctttcc cgtcttacca agcgttaggt ggacacaaaa caagtcaccg gaaaccggtt 360agtgtcgatg ttaataatag taacggaacc gttactaata acggaaatat tagtaacggt 420ttagttggtc aaagtgggaa gactcataac tgctctatat gttttaagtc gtttccctct 480ggtcaagcat tgggtggtca caaacgttgt cactatgatg gtggtaacgg taacagtaac 540ggtgacaata gccacaagtt tgacctaaat ttaccggctg atcaagttag tgatgagaca 600attggaaaaa gtcaactctc cggtgaagaa acaaagtcgg tgttgtga 648229834DNAPetunia x hybrida 229atggctcttg aagctttaaa atcacccaca gcagcaacac caactctacc accacgttat 60gaagatcaag ttgatatgag taatttggat tcatgggtta aaggaaaacg atccaaacga 120cctcggattg aaaccccacc ttctgaagaa gaatatctag cactttgtct tatcatgctt 180gcccgtagcg gtaacggaac tacacccagt tcaattcccg gttcaactga tacaactact 240atttctaaag aaccggagaa gaaaaaccgt gacgtggcac cggtttatca agaaacagaa 300caatcttaca agtgtagcgt gtgtgacaag tcttttagtt cttatcaagc tcttggcgga 360cacaaagcaa gtcataggaa aattacaact attgccacca ccgccttatt agatgacaac 420aacaataatc ctacaacgtc aaattctaca aatggcaacg ttgttaataa tatttctact 480ttgaacccaa gtggacgttc acacgtgtgt tctatatgtc acaaggcttt tccaagtgga 540caagctttag gtggacacaa gcgccgtcac tatgaaggca aacttggcgg caacaacaac 600aacaaccacc gtgacggcgg tggtcatagt ggaagtgttg tgacaacttc tgatggtggc 660gcgtctactc acacgctccg tgactttgac ttgaacatgt tgcctccttc cccagagttg 720caattggggt tgagtattga ctgtggtttg aaaagtcaac aagttcctat tgaacaagaa 780gttgaaagtc ctatgccttt gaagaaaccg cgtttattgt tttccatgga ttga 834230738DNAArabidopsis thaliana 230atggctctcg agactctcaa ttctccaaca gctaccacca ccgctcggcc tcttctccgg 60tatcgtgaag aaatggagcc tgagaatctc gagcaatggg ctaaaagaaa acgaacaaaa 120cgtcaacgtt ttgatcacgg tcatcagaat caagaaacga acaagaacct tccttctgaa 180gaagagtatc tcgctctttg tctcctcatg ctcgctcgtg gctccgccgt acaatctcct 240cctcttcctc ctctaccgtc acgtgcgtca ccgtccgatc accgagatta caagtgtacg 300gtctgtggga agtccttttc gtcataccaa gccttaggtg gacacaagac gagtcaccgg 360aaaccgacga acactagtat cacttccggt aaccaagaac tgtctaataa cagtcacagt 420aacagcggtt ccgttgttat taacgttacc gtgaacactg gtaacggtgt tagtcaaagc 480ggaaagattc acacttgctc aatctgtttc aagtcgtttg cgtctggtca agccttaggt 540ggacacaaac ggtgtcacta tgacggtggc aacaacggta acggtaacgg aagtagcagc 600aacagcgtag aactcgtcgc tggtagtgac gtcagcgatg ttgataatga gagatggtcc 660gaagaaagtg cgatcggtgg ccaccgtgga tttgacctaa acttaccggc tgatcaagtc 720tcagtgacga cttcttaa 738231846DNASolanum tuberosum 231ctcacaaaac ttctcaagtt ttataatatt cttaacactc tctctctcta aacaaaacag 60acgaataatt caatcaaatt gatattgaga tggcccttga agctttgaat tctccaactg 120gtacttcaaa tccgcagacg tttaaatttg agagcaaagg ccagcagcag cttcggtacc 180ttgagaattg gactaaaggg aagagatcta agaggtcacg gagtatggaa cgccagccga 240ctgaagagga atatttggcg atttgtttga ttatgcttgc gcgtagcgat ggctctgtta 300atcaggtacg atctctacca ccgccggtgc cagtgatgaa aatccacgcg ccgtcggaga 360agatggagta taagtgttcg gtttgtggta agggatttgg atcttatcaa gctttaggag 420gacataaagc tagtcaccgg aaactcatcg ccggcgtcag cggcggcgga gatgatcagt 480caactacctc tactactact aacgctaccg gaactactag ctccggtaac ggtaacggta 540gtggaaggac tcacgagtgt tcgatttgtc acaagtgttt tcctactgga caagctttgg 600gaggacacaa acggtgtcac tacgacggtg gtaacggtaa cggaaacgct aacagtagcg 660ttagcgctag cgtcggagtt acgtcgtcgg agggcgtggg gtcaacaatc agccaccgtg 720attttgactt gaacatcccg gcgttgccgg aattctggcc tggatttggt tccggcgagg 780atgaggtgga gagtccacat ccggcgaaga aatcacggct atctctacct ccaaaatttg 840aattat 846232819DNAGossypium raimondii 232ttacttttag ctctctgttt ttgctctcaa actcgaaggg attcggaagt atttgatatg 60gcgcttgaag ctctgaactc gccggcgacg cctttcacca acaaatacga tgacgtggac 120aacaattacg tcgagacatg gaagaaaggc aagcgttcga agcgacaacg tggcgactct 180cctgctgctg ttgaacttca acccaccacc gaagaagagt acctcgctct ttgtctcatc 240atgctcgctc gcggctcttc cggtgctgat cttgatgtta ttcgtcggtc ttcctcttcg 300tcgtcaccgc ctccgccgcc gcctgctttg aagttgtctt acaagtgtag tgtttgtgac 360aaggcgttcc cttcttatca agctttgggc ggtcataaag ccagccaccg caaacccctt 420tccgccgacg ccgctaccac caccgccgcc gccaacgtcg ataacccatc aacaaccagc 480accgccacca ccgccaccag cagcggtagg cttcacgagt gttccatctg ccacaagagt 540ttccctacag gccaagcctt gggtggtcat aaacgctgcc actacgaagg tggcaacaac 600aacaacaaca acaacaaaaa taacaacaac agcggtagcg ttagcgttag cggggtgact 660tcttcggatg ggggcgcgtt gagccacaac caccgtgcag tcgactttga ctttgacctc 720aacttgccgg ccttgccgga gttcagtcaa atgtacccag atgaagaaga ggtccaaagc 780ccattgccga cccagaaacc acgtttcttg atcgccaag 819233705DNAVitis aestivalis 233cctctttcat ggctctggaa gctctcaact caccaaccac acccacgcct tcctttcact 60acgaacaacc cagcctccac tctctggagt catgggccaa gcgcaagcgt tccaagcgtc 120ctcgcttcga caaccaacct acagaggaag agtatctggc tctctgcctc atcatgttgg 180ctcgaggagg cgccgcctcc tccaccgtct cacaccgccg ccatctctct ccccctcctg 240ccctgcaggt ggaagctcct aaactcacat acaaatgttc agtttgtaac aaggccttcg 300catcctacca ggcactaggg ggacacaagg ccagccaccg taagcagtcc ggatccgatg 360acctgtcggc ctccatcacc accacaagca ccgcggccgc tgccagcggt ggtaggactc 420atgagtgttc catctgtcac aagactttcc ccactggaca ggctttgggt ggacacaagc 480gatgccacta cgaaggcggc gccagcgtca gcagtggcgt tacctcctcc gaaggtgtgg 540ggtcaaccca cagccaccgt gacttcgacc tcaacctgcc ggcctttccc gaattatggt 600ccgcacgtcg attcccagtc gatgacgagg tcgagagtcc tctaccgaca aagaagcccc 660gtctccagat gctgccgcca aaaaccgaaa ttctctcaga ttacc 705234742DNALycopersicon esculentum 234acactctcga taactctgaa cgaacaattt gaattaaatt gatatcgaaa tggctcttga 60agctttgaat tctccaactg gtactacttc aaatccgcaa acgtttcaat ttgagagcaa 120aggccagcag cagcttcggt accttgagaa ttggactaaa gggaagagat ctaagaggtc 180acggagtatg gatcgtcaac cgactgaaga ggaatatttg gcgctttgtt tgattatgct 240tgcaaggagc gatggctctg ttaatcacgt acggtctcta ccaccgccgg tgccagtgat 300gaaaattcac gagacggcgg agaagatgtt gtataggtgt tcggtttgtg gtaagggatt 360cggatcttat caagctttag gaggacataa agctagtcac cggaaactca tcgccggcgg 420agatgatcag tcgactacct ctactactac gaacgctaac ggaactacaa gctccggtaa 480cggtaacggt aacggtagtg gaactggaag gactcacgag tgttcgattt gtcacaagtg 540ttttccgact gggcaagctt tgggaggaca caaacggtgt cactacgacg gtggtaacag 600taacggtaac ggaaatgcta acgctaacag tagcattagc gctagcgtcg gagttacgtc 660gtcggagggc gtgggttcaa caatcagcca ccgtgatttt gacttgaaca ttccggcgtt 720gccgggattc tggcctggat tt 742235804DNAMedicago truncatula 235cgctactcct ttccaccctt tgaggaacca aatctgagtt atcttgaaac accgtggacg 60aaaggtaaac gttcaaagcg ttctcgtatg gatcaatctt catgcactga agaagagtat 120ctcgctcttt gtctcatcat gcttgctcgc agcggaaaca acaacgacaa caagactgaa 180tcggtgccgg tgccggcgcc gctaaccacc gttaaactca gtcacaaatg ctcagtctgc 240aacaaagctt tttcatctta tcaagcccta ggtggacaca aagccagtca ccggaaagct 300gttatgtccg caaccaccgt tgaagatcag accaccacca cttcatctgc cgtgactacc 360agctctgcat ccaacggtaa gaacaagact catgagtgtt ccatctgtca caaatccttt 420cctactggac aggctttggg aggacacaag cgttgtcact acgaaggcag cgttggtgcc 480ggtgccggaa gtagcgctgt aactgccgcc tctgaaggag ttggatcgtc tcacagtcac 540caccgtgatt ttgatcttaa cctaccggct tttccggact tttcaaagaa gtttttcgtg 600gatgacgagg tttctagtcc tttacctgct gcaaagaagc cctgtctttt caagcttgaa

660attccttctc attactgatc aataatagat ccaattttat tgttattatt agtaataatt 720attatcgctt agggcatagt tattttcttt tttctttcaa tattttggat caatttgttc 780tgtacataca aattgggact ggct 804236743DNAPopulus sp.misc_feature(680)..(680)n is a, c, g, or t 236tccctcctcc ctgtctctct caataccttt cttcctctcc ttggaaatct tgttcgtttg 60tacccctcgt tgcccactca cacaagaaaa actcagtatc aagaatccaa atcatggctc 120ttgaagctct gaactctcct acaacagccg ctcctttaaa ttatgaagaa acatggatta 180agaggaaacg ctctaagaga cctcgtagtg agtccccttc gaccgaggaa gaatacctcg 240ctttttgcct tatcatgctt gctcgtggcg gctccactgc cgcaaccgcc aaaaaaaccg 300cttccgcctc ccctgcacca ccccaaccac caactttgga tctttcttac aagtgtacgg 360tttgcaacaa ggctttctct tcttaccagg ctctcggcgg gcacaaagcc agtcacagga 420aatcctcctc cgagtcaacc gtcgccacag ccgctgaaaa cccatcagcc tccaccacaa 480ctaacacaac caccacgacc accaatggta ggactcatga gtgttctatc tgccacaaga 540ctttccttac tggacaggcc ttaggcggac acaaacgttg tcactatgag ggcacaattg 600gaggcaacaa cagcagcagt gctagcgctg caatcaccac ctcagacggt ggtgctgttg 660gaggcggtgg cgtgagccan agtaagagtc aaagaagcgg tggtggattt gactttgacc 720tgaacttgcc tgctttgcct gaa 743237894DNACitrus sinensismisc_feature(872)..(874)n is a, c, g, or t 237ggggatcatt tccatccatt tgaaatcacc tctctctctc tctcgaaact caacagattc 60aaccacaaga gtcgagaatt cactaacaag aaaagaaaaa attcacgact atggctctag 120aagctctaaa ctcccctaca acaagcaccc acaatcatca tccgtttaga tacgaagact 180catggactaa aagaaggcgc tcaaaacgac tccgtaccga cgagtcccca caactaccag 240cagcagcagc agctcccacg gaagaagagt acatggctct ctgtctcatc atgctcgccc 300gcggtaccac caccgccaac accgctccgg ccgaaagaac cccaaccctg gcgccggaac 360agaagccgct ggatcagttc ccggagccgc caagtttgaa actctcttac aagtgcagtg 420tgtgcaacaa ggctttctct tcgtaccagg cccttggtgg gcacaaagcc agccaccgca 480agaacgctgc tgacgcctcg gcttctccca acgccgcagc cgccagtgac gtcaccccac 540caccgtcggc aacggcgagc agcggaagcg gtggtaggac ccacgagtgc tctatttgtc 600acaagtcttt tcccacggga caagctctag gaggccacaa gcggtgtcac tatgaaggcg 660gcatcaacaa caacaacaac agcagcagca ataataataa aagtaataat aatagcgacg 720ttgttactag tggtagcgct agcgttggtg caagtgcggt gacgttttcg gaaggcggag 780ggagcagcag tcaacgcgga tttgacctga acttgccggc gttgccggag ttttggtcac 840aggaagttga gtctccgttg ccggccaaga annncaagtt gttgatgcac tact 894238900DNALotus corniculatus 238tcaccaacca ccgccgctcc aaccttcact cccttccagg aaccgaacca cagctacatg 60gtcgacgcac cgtgggcgaa gagaaagcgt tccaagcgtt ctcgcacgga cagccatcac 120aaccacgctt cctgcacgga ggaagagtat ctcgctctct gcctcatcat gctcgctcgc 180ggcagcaccg ccgtaacacc caagctcact ctgtctcgtc cggcaccggt aaccgctgaa 240aagctcagtt acaagtgctc tgtctgcgaa aaaaccttcc cctcttacca agcccttggc 300ggacacaagg ccagccaccg gaaactcgcc ggcgccgccg ctgaggacca ctccacctcc 360tccgccgtga ccaccagctc tgcctccaac ggtggaggca aggtccacca gtgctccatc 420tgccagaaat ccttccctac aggacaggcc ttgggaggac acaagcgttg ccactatgaa 480ggcggtggtg gtgctagtag caccgccaca gccaccgcat cggaaggtgt gggatccaca 540cacagccacc agcgcaactt cgatctgaac ctgccggctt ttccggactt ctccgccagc 600aaatttttcg tggaagaaga ggtttccagt cccctgcctt cgaagaagcc acgtcttttg 660cccaagattg aaatccctca ttattattaa ttaattaatg catttagatc aaagttaatt 720agtgcttaat tagcttttca attgtttggg aattgggatt tgatttgttc ttgtacatat 780acagtagcta gctagctgga cttggagctt attagctggt tttaggattt cttctacatt 840gtattttgac agtcattcat tatcatataa ttcaattcat ttattcaatc tatctattta 9002391178DNALolium perennemisc_feature(681)..(681)n is a, c, g, or t 239ggctggtaaa acaaatataa gtattaatat aaatataata caatagaagg aaaataaata 60aaatttccct ctgtgccgtg caaaaatgca cggcaatggg ctggcccgca cggcaaaggc 120atcgttgccg tgtccacggc aatgggttgg cccgcacggc aaaggcatcg ttgccgtgtc 180cacgtcttcg ccgtgcgcct tggctctatc tttgccgtga agcgttcttt gccgtgtgcc 240ttttatttct ttgccgtggg atgctgcctt tgccgagcgc tgagctggcg ctttgccgtg 300cgcgtattgt ttgccgtgcg tcgtcccaga gctgtacggc aaagaattca ctgccgtgca 360cgagacacac gggaaagaag ttttgcatgg caaagggcgc tgacagcaca cggcaaagag 420cctggcacgg cattgagctt ttttcccgta atgatagacg gcataatata atggacgcac 480atgctgatgt caggatgtca cccactcatc ctagtatttg tgggacgtga attctttgtg 540agatgggcaa tggggtgtga acaaaataag ttttgtacta gtagataaac atttttaccc 600ataaacaatt gttctgtatt gaatgagaaa ttattttgta ctggatgaaa attttctgag 660taactgtgta agattaacat naatcaagag acaaatccaa tggctacaaa gtcaactaat 720acttgttaaa agttccgata cttaaaatta tcaaaactga tatatagaat attgcccatc 780tcgccaccgt gctagtttaa cagacgatgg acgaatatca gtcttgtatt ggataatcga 840tgcatgcgag ctatcggcca cctgtccatg cttccagaag gagccgagac gtggcgactt 900cgtccgacgc gccgactatc tgcacacgcc cggcttctcg tcgtgggcga gtcagcagtt 960acgggctttc cgcctaccaa ctcacacgta gcgccctatc gtggcgcttg atcgatgcaa 1020cagcgatgcc tatcccagct cctcaagctg cttataagta tgtcctcggc catcactgct 1080tacacaacaa acacagctac ttatcgcagt gtactaaaca agacgtacta gctagatttc 1140gtgaggtaaa atcagtgcaa tatcacttgt gcaagatg 11782405214DNAArtificialgenetic construct 240ggcgcgcctt aattaacggg ctggtaaaac aaatataagt attaatataa atataataca 60atagaaggaa aataaataaa atttccctct gtgccgtgca aaaatgcacg gcaatgggtt 120ggcccgcacg gcaaaggcat cgttgccgtg tccacggcaa tgggttggcc cgcacggcaa 180aggcatcgtt gccgtgtcca cgtctttgcc gtgcgccttg gctctatctt tgccgtgaag 240cgttctttgc cgtgtgcctt ttatttcttt gccgtgggat gctgcctttg ccgagcgctg 300agctggcgct ttgccgtgcg cgtattgttt gccgtgcgtc ctcccagagc tgtacggcaa 360agaattcatt gccgtgcacg aggcacacgg gaaagaagtt tcgcatggca aagggcgctg 420acagcacacg gcaaagagcc cggcacggca ttgagctttt tttcccgtaa tgatagacgg 480cataatataa tggacgcaca tgctgatgtc aggatgtcac ccactcatcc tagtatttgt 540gggacgtgaa ttctttgtga gatgggcaat gggatgtgaa caaaataagt tttgtactag 600tagataaaca tttttaccca taaacaattg ttctgtattg aatgaaaaat tattttgtac 660tggatgaaaa tcttctgagt aactgtgtaa gattaacatg aatcaagaga caaatccaat 720ggctacaaag tcaactaata cttgttaaaa gttccgatac ttaaaattat caaaactgat 780atatagaata ttgcccatct cgccaccgtg ctagtttaac agacgatgga cgaatatcag 840tcttgtattg gataatcgat gcatgcgagc tatcggtcac ctgtccatgc ttccagaagg 900agccgagacg tggcgacttc gtccgacgcg ccgactatct gcacacgccc ggcttctcgt 960cgtgggcgag tcagcagtca caggctttcc gcctaccaac tcacacgtag cgccctatcg 1020tggcgcttga tcgatgcaac agcgatgcct atcccagctc ctcaagctgc ttataagtat 1080gtcctcggcc atcactgctt acacaacaaa cacagctact tatcgcagtg tactaaacaa 1140gacgtactag ctagatttcg tgaggtaaaa tcagtgcaat atcacttgtg caagccatta 1200gttccgtcgc catggcgtcc ccggagggcg ccaactgggt cttcgactgc ccgctcatgg 1260acgaccttgc tgccgccgac ttcaccgcac cgcccgcagg aggcttctac tgggcaccac 1320cgatgcagcc gcagatgcac acccaggccc cggccgtctc cgccaccccg cctcccaacc 1380actgtgccga aatcaatagc cctatttctg tggactggga ccatgccaaa ggacagccaa 1440caaataaacg tcctaggtca gaatctggtg ctcaacccag ctccaaagca tgcagggaga 1500aagcgagaag ggacaagcta aacgagaggt tcttggaatt gggtgctgtc ttggatccag 1560ggaaaacacc taaaatcgac aagtgtgcta tattgaatga tgctattcgt gcggtgactg 1620agctacgtag tgaagcagag aagctgaagg attcaaacga gtctctccaa gagaagatca 1680aagagctgaa ggctgagaag aatgagctgc gggatgagaa gcaaaagctg aaggcagaga 1740aagagagcct ggagcagcag atcaagttca tgaatgcccg tcagagcctc gtaccacacc 1800taccgcaccc ttcggttatc ccagcggctg catttgctgc tccccaaggg caagtgccag 1860ggcagaagct gatgatgcct gtcattggct accatggatt tcccatgtgg caattcatgc 1920caccttctga tgttgatacc tccgatgatc ccaagtcgtg ccctcctgtt gcataagcca 1980gctaaaggcc tggtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg 2040ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat 2100ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa 2160tccagatccc ccgaattaat tcggcgttaa ttcagtatcg gcgcgcctta attaaaatcg 2220aatttcgacc atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag 2280tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 2340ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 2400taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 2460aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 2520attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 2580cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 2640gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 2700ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 2760agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2820tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 2880ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 2940gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 3000ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3060gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 3120aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 3180aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3240ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3300gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 3360gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 3420tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 3480ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 3540ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 3600atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 3660ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 3720tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 3780attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3840tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 3900ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 3960gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 4020gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 4080gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 4140aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 4200taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 4260tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 4320tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 4380atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 4440tttccccgaa aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa 4500ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 4560taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 4620gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 4680aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 4740gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 4800cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 4860gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 4920cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat 4980tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 5040tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 5100cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg 5160gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt gatt 52142415118DNAArtificialgenetic construct 241ggcgcgcctt aattaacggg ctggtaaaac aaatataagt attaatataa atataataca 60atagaaggaa aataaataaa atttccctct gtgccgtgca aaaatgcacg gcaatgggtt 120ggcccgcacg gcaaaggcat cgttgccgtg tccacggcaa tgggttggcc cgcacggcaa 180aggcatcgtt gccgtgtcca cgtctttgcc gtgcgccttg gctctatctt tgccgtgaag 240cgttctttgc cgtgtgcctt ttatttcttt gccgtgggat gctgcctttg ccgagcgctg 300agctggcgct ttgccgtgcg cgtattgttt gccgtgcgtc ctcccagagc tgtacggcaa 360agaattcatt gccgtgcacg aggcacacgg gaaagaagtt tcgcatggca aagggcgctg 420acagcacacg gcaaagagcc cggcacggca ttgagctttt tttcccgtaa tgatagacgg 480cataatataa tggacgcaca tgctgatgtc aggatgtcac ccactcatcc tagtatttgt 540gggacgtgaa ttctttgtga gatgggcaat gggatgtgaa caaaataagt tttgtactag 600tagataaaca tttttaccca taaacaattg ttctgtattg aatgaaaaat tattttgtac 660tggatgaaaa tcttctgagt aactgtgtaa gattaacatg aatcaagaga caaatccaat 720ggctacaaag tcaactaata cttgttaaaa gttccgatac ttaaaattat caaaactgat 780atatagaata ttgcccatct cgccaccgtg ctagtttaac agacgatgga cgaatatcag 840tcttgtattg gataatcgat gcatgcgagc tatcggtcac ctgtccatgc ttccagaagg 900agccgagacg tggcgacttc gtccgacgcg ccgactatct gcacacgccc ggcttctcgt 960cgtgggcgag tcagcagtca caggctttcc gcctaccaac tcacacgtag cgccctatcg 1020tggcgcttga tcgatgcaac agcgatgcct atcccagctc ctcaagctgc ttataagtat 1080gtcctcggcc atcactgctt acacaacaaa cacagctact tatcgcagtg tactaaacaa 1140gacgtactag ctagatttcg tgaggtaaaa tcagtgcaat atcacttgtg caagccatta 1200gtatggccgt ggaggcggtt ctcgaagcgg cggcgatgat acagtcgccg ccgagcaaga 1260agatggaggc gtctagtagc agcgacgagg cgttcgaggc gttgcagcag cacacggagg 1320ggtggtccaa gaagaagcgc tcgaggcggc cacgggcgct cgagcccagc gaggaggagt 1380acctcgcgtt ctgcctcgtc atgctggcgc gcggccaccg cgacgccgcg ccggagcacg 1440ggtgctccgt ctgcggcaag gcgttcgcgt cgtaccaggc gctcggcggc cacaaggcca 1500gccaccggaa gccacccaca gctccagccg cggtggcagc aagcgccgtc cccgaggagg 1560acaagccacg ggcggctgcc tcgtcctcgt ctgggtccgg cgatgccgct ggcggcggca 1620aggtccacga gtgcaacgtg tgccagaaga cgttcccgac ggggcaggcg ctgggcggcc 1680acaagcggtg ccactacgac ggcaccatcg gcagcgccgc cgcgcccacg gtgaaggctg 1740ccaaggccgc cgccgcggcg agcgcgccga cggcgacgaa ccgggggttc gacctgaacg 1800tgccggcgct gccgggactc gcggaggagg gggaggaggt gctcagcccg gtatccttca 1860agaagccgag gctcatgatc accgcgtgaa ggcctggttt ctccataata atgtgtgagt 1920agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa 1980gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc 2040taaaaccaaa atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt 2100atcggcgcgc cttaattaaa atcgaatttc gaccatatgg gagagctccc aacgcgttgg 2160atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag 2220ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 2280ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 2340tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 2400cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 2460ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 2520ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 2580gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 2640gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 2700taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 2760accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 2820tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 2880cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 2940agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 3000gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 3060gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 3120tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 3180acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 3240cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 3300acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 3360acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 3420tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 3480ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 3540ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 3600tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 3660aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 3720ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 3780ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 3840gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 3900gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 3960cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 4020actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 4080ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 4140tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 4200ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 4260agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 4320aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat 4380accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg 4440ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 4500ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 4560tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 4620tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 4680tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 4740aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 4800ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 4860ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 4920gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 4980gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat 5040acgactcact atagggcgaa ttgggcccga cgtcgcatgc tcccggccgc catggcggcc 5100gcgggaattc gattgatt 511824210821DNAArtificialgenetic construct 242gaatttctag ttctagatgc atgctcgaaa ttcgattggc gcgccttaat taataagagc 60agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag 120tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc 180tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg 240gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg 300acagtggtcc caaagatgga

cccccaccca cgaggagcat cgtggaaaaa gaagacgttc 360caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga cactctcgtc 420tactccaaga atatcaaaga tacagtctca gaaggccaaa gggctattga gacttttcaa 480caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc 540aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag 600gctatcgttc aagatgctct gccgacagtg gtcccaaaga tggaccccca cccacgagga 660gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata 720tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta 780tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa 840atctatctct ctccattagt tccgtcgcca tggcgtcccc ggagggcgcc aactgggtct 900tcgactgccc gctcatggac gaccttgctg ccgccgactt caccgcaccg cccgcaggag 960gcttctactg ggcaccaccg atgcagccgc agatgcacac ccaggccccg gccgtctccg 1020ccaccccgcc tcccaaccac tgtgccgaaa tcaatagccc tatttctgtg gactgggacc 1080atgccaaagg acagccaaca aataaacgtc ctaggtcaga atctggtgct caacccagct 1140ccaaagcatg cagggagaaa gcgagaaggg acaagctaaa cgagaggttc ttggaattgg 1200gtgctgtctt ggatccaggg aaaacaccta aaatcgacaa gtgtgctata ttgaatgatg 1260ctattcgtgc ggtgactgag ctacgtagtg aagcagagaa gctgaaggat tcaaacgagt 1320ctctccaaga gaagatcaaa gagctgaagg ctgagaagaa tgagctgcgg gatgagaagc 1380aaaagctgaa ggcagagaaa gagagcctgg agcagcagat caagttcatg aatgcccgtc 1440agagcctcgt accacaccta ccgcaccctt cggttatccc agcggctgca tttgctgctc 1500cccaagggca agtgccaggg cagaagctga tgatgcctgt cattggctac catggatttc 1560ccatgtggca attcatgcca ccttctgatg ttgatacctc cgatgatccc aagtcgtgcc 1620ctcctgttgc ataagccagc taaaggcctg gtttctccat aataatgtgt gagtagttcc 1680cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc 1740cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac 1800caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtatcggc 1860gcgccttaat taaaatcgaa tttcgaccat actagtggat ccccctcgga ctagaagctt 1920ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 1980tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 2040tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga 2100tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg 2160ggtaaaccta agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa 2220ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa 2280agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag 2340ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg 2400cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac 2460tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc 2520cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc 2580tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc 2640aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg 2700gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc 2760ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg 2820accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc 2880gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg 2940gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg 3000gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa 3060gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc 3120gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg 3180acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg 3240ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg 3300gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc 3360gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg 3420cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc 3480tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc 3540cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg 3600cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac 3660gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc 3720ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt 3780gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg 3840cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg 3900cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga 3960gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct 4020tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa 4080atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta 4140agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac 4200acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag 4260atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag 4320ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg 4380cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg 4440aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg 4500aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg 4560gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc 4620aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc 4680gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg 4740gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc 4800gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg 4860tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg 4920ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga 4980accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg 5040acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa 5100cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg 5160gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga 5220gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga 5280tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc 5340ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca 5400gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct 5460gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg 5520aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag 5580catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa 5640aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca 5700ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca 5760tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac 5820ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg 5880aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc 5940gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac 6000cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc 6060tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 6120gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 6180ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat 6240actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 6300aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc 6360tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 6420cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 6480gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 6540gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 6600gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 6660ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 6720atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 6780tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 6840ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 6900gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 6960ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7020ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7080agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7140ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt 7200actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc 7260ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc 7320ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa 7380agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa 7440agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt 7500taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta 7560agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt 7620cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt 7680gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc 7740tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc 7800atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg 7860tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca 7920ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata 7980ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca 8040agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa 8100ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac 8160ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca 8220acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt 8280ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg 8340ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg 8400gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga 8460caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg 8520gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag 8580tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac 8640cctataggaa ccctaattcc cttatctggg aactactcac acattattat ggagaaactc 8700gagcttgtcg atcgacagat ccggtcggca tctactctat ttctttgccc tcggacgagt 8760gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc 8820cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc 8880gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata 8940gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc 9000cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca 9060gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat 9120gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac 9180gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc 9240gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc 9300aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg 9360gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac 9420cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc 9480acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg 9540aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc 9600ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg 9660agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag 9720aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt gcccccccgg 9780atctgcgaaa gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc 9840tctccaaatg aaatgaactt ccttatatag aggaaggtct tgcgaaggat agtgggattg 9900tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt 9960tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact 10020gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt 10080gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc 10140gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga 10200gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttatcaca 10260tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt 10320gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc 10380tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa 10440gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa 10500agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag 10560agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa tacgcaaacc gcctctcccc 10620gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 10680agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 10740tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 10800aacagctatg accatgatta c 1082124310905DNAArtificialgenetic construct 243gaatttctag ttctagatgc atgctcgaaa ttcgattggc gcgccttaat taataagagc 60agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag 120tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc 180tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg 240gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg 300acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc 360caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga cactctcgtc 420tactccaaga atatcaaaga tacagtctca gaaggccaaa gggctattga gacttttcaa 480caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc 540aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag 600gctatcgttc aagatgctct gccgacagtg gtcccaaaga tggaccccca cccacgagga 660gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata 720tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta 780tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa 840atctatctct ctccattagt ttgcccactg cttgctgcta ctctctcgat ctgtagttgc 900tcaggtgtgc aagaaagata ctcacacgcg agcttgcttg gcatggccgt ggaggcggtt 960ctcgaagcgg cggcgatgat acagtcgccg ccgagcaaga agatggaggc gtctagtagc 1020agcgacgagg cgttcgaggc gttgcagcag cacacggagg ggtggtccaa gaagaagcgc 1080tcgaggcggc cacgggcgct cgagcccagc gaggaggagt acctcgcgtt ctgcctcgtc 1140atgctggcgc gcggccaccg cgacgccgcg ccggagcacg ggtgctccgt ctgcggcaag 1200gcgttcgcgt cgtaccaggc gctcggcggc cacaaggcca gccaccggaa gccacccaca 1260gctccagccg cggtggcagc aagcgccgtc cccgaggagg acaagccacg ggcggctgcc 1320tcgtcctcgt ctgggtccgg cgatgccgct ggcggcggca aggtccacga gtgcaacgtg 1380tgccagaaga cgttcccgac ggggcaggcg ctgggcggcc acaagcggtg ccactacgac 1440ggcaccatcg gcagcgccgc cgcgcccacg gtgaaggctg ccaaggccgc cgccgcggcg 1500agcgcgccga cggcgacgaa ccgggggttc gacctgaacg tgccggcgct gccgggactc 1560gcggaggagg gggaggaggt gctcagcccg gtatccttca agaagccgag gctcatgatc 1620accgcgtgat ttgaccatac aatctgcata tagttggtca aaatcaaggg ttcttctgta 1680gcttagcttc tgttagtgat tgccgtacat agattgttgg tgattgaagg cctggtttct 1740ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct 1800catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc 1860aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta 1920attcggcgtt aattcagtat cggcgcgcct taattaaaat cgaatttcga ccatactagt 1980ggatccccct cggactagaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga 2040aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 2100taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 2160atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc 2220agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa 2280cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa 2340ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca 2400gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa 2460gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt 2520cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc 2580gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa 2640cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc 2700aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg 2760acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag 2820cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc 2880acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc 2940ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt 3000ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag 3060gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac 3120cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc 3180cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag 3240gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga 3300tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg 3360tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca 3420gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa 3480acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca 3540aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac 3600catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga 3660ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac 3720cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga 3780cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc 3840agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga 3900cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt 3960tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc 4020cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac 4080tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt 4140ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa 4200atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct 4260gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg 4320gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag 4380ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag 4440atgaatttta gcggctaaag

gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc 4500cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt 4560ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca 4620aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga 4680aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt 4740ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc 4800cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct 4860atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga 4920agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg 4980tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg 5040tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc 5100gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa 5160agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc 5220agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga 5280ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc 5340tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc 5400accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc 5460gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca 5520gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc 5580tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct 5640accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag 5700ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt 5760acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc 5820cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt 5880tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat 5940aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc 6000gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg 6060ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc 6120gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc 6180tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 6240caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag 6300tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac 6360tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 6420tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6480gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6540caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6600tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6660gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6720ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6780cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6840tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6900tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 6960cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7020agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 7080agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7140gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7200aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7260ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta 7320ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt 7380ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc 7440tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc 7500tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa 7560aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca 7620catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat 7680tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca 7740gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat 7800cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg 7860gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg 7920tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca 7980gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga 8040tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt 8100tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact 8160gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa 8220gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc 8280aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa 8340cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc 8400cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt 8460gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt 8520ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac 8580tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt tggttttagg 8640aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt 8700atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc tgggaactac 8760tcacacatta ttatggagaa actcgagctt gtcgatcgac agatccggtc ggcatctact 8820ctatttcttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc 8880tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt 8940cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa 9000attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg 9060gagtcgtggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc 9120catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc 9180gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt 9240gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag 9300catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg 9360ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg 9420accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc 9480gatcgcatcc atagcctccg cgaccggttg tagaacagcg ggcagttcgg tttcaggcag 9540gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa 9600ctccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac 9660ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc 9720tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga 9780gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt 9840tttcatatct cattgccccc ccggatctgc gaaagctcga gagagataga tttgtagaga 9900gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag 9960gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca 10020atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg 10080tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt 10140atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg 10200acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt 10260ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt 10320gtcgtgctcc accatgttat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc 10380ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga 10440ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc 10500cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt 10560tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc 10620tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag 10680ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 10740aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 10800cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 10860agcggataac aatttcacac aggaaacagc tatgaccatg attac 109052447PRTArtificialmotif 244Gln Ala Leu Gly Gly His Lys1 5245120PRTTriticum aestivum 245Met Ala Ser Pro Glu Gly Ser Asn Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Ala Ala Asp Phe Ala Ala Ala Pro Ala Gly Gly Phe 20 25 30Tyr Trp Thr Pro Pro Met Gln Pro Gln Met His Thr Leu Ala Gln Ala 35 40 45Val Ser Ala Thr Pro Ala Pro Asn Pro Cys Ala Glu Ile Asn Ser Ser 50 55 60Val Ser Val Cys Trp Asp His Ala Lys Gly Gln Pro Lys Asn Lys Arg65 70 75 80Pro Arg Ser Glu Thr Gly Ala Gln Pro Ser Ser Lys Ala Cys Arg Glu 85 90 95Lys Ala Arg Arg Asp Lys Leu Asn Glu Arg Phe Leu Glu Ser Ser Ala 100 105 110Asp Leu Asp Pro Gly Asn Thr Pro 115 120246135PRTSecale cerealemisc_feature(86)..(86)Unknown 246Met Ala Ser Pro Glu Gly Ser Asn Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Ala Ala Asp Phe Ala Ala Ala Pro Ala Gly Gly Phe 20 25 30Tyr Trp Thr Pro Gln Met His Thr Leu Ala Gln Ala Val Ser Ala Thr 35 40 45Pro Ala Pro Asn Gly Gly Ala Glu Ile Asn Ser Ser Val Ser Val Asp 50 55 60Cys Asp His Val Lys Gly Gln Pro Lys Asn Lys Arg Pro Arg Ser Glu65 70 75 80Thr Gly Ala Gln Pro Xaa Ser Lys Ala Cys Arg Glu Lys Val Arg Arg 85 90 95Asp Lys Leu Asn Glu Arg Phe Leu Glu Leu Gly Ala Val Leu Asp Pro 100 105 110Gly Lys Thr Pro Lys Ile Asp Lys Cys Ala Ile Leu Asn Asp Ala Ile 115 120 125Arg Ala Val Thr Glu Leu Arg 130 135247151PRTOryza sativa 247Met Ala Ser Pro Glu Gly Ser Thr Trp Val Phe Asp Cys Pro Leu Met1 5 10 15Asp Asp Leu Ala Ala Ala Ala Gly Phe Asp Ala Ala Pro Ala Gly Gly 20 25 30Phe Tyr Trp Thr Thr Pro Ala Pro Pro Gln Ala Ala Leu Gln Pro Pro 35 40 45Pro Pro Gln Gln Gln Pro Val Ala Pro Ala Thr Ala Ala Pro Asn Ala 50 55 60Cys Ala Glu Ile Asn Gly Ser Val Asp Cys Glu His Gly Lys Glu Gln65 70 75 80Pro Thr Asn Lys Arg Pro Arg Ser Glu Ser Gly Thr Arg Pro Ser Ser 85 90 95Lys Ala Cys Arg Glu Lys Val Arg Arg Asp Lys Leu Asn Glu Arg Phe 100 105 110Leu Glu Leu Gly Ala Val Leu Glu Pro Gly Lys Thr Pro Lys Met Asp 115 120 125Lys Ser Ser Ile Leu Asn Asp Ala Ile Arg Val Met Ala Glu Leu Arg 130 135 140Ser Glu Ala Gln Lys Val Glu145 150

Claims

1. An isolated polynucleotide comprising:a) a sequence encoding a polypeptide with at least 95% identity to the amino acid sequence of SEQ ID NO:163; orb) the complement of the sequence of a).

2. The isolated polynucleotide of claim 1, wherein the polypeptide is derived from a plant species and comprises the sequence of SEQ ID NO:160.

3. The isolated polynucleotide of claim 1, wherein the polypeptide is derived from a dicotyledonous species and comprises the sequence of SEQ ID NO:161.

4. The isolated polynucleotide of claim 1, wherein the polypeptide is derived from a monocotyledonous species and comprises the sequence of SEQ ID NO:162.

5. The isolated polynucleotide of claim 1, wherein the polypeptide has the amino acid sequence of SEQ ID NO:163.

6. The isolated polynucleotide of claim 1, wherein the sequence encoding the polypeptide in a) has at least 95% identity to the sequence of SEQ ID NO:201.

7. The isolated polynucleotide of claim 1, wherein the sequence encoding the polypeptide in a) has at least 95% identity to the coding sequence of SEQ ID NO:201.

8. The isolated polynucleotide of claim 1, wherein the sequence encoding the polypeptide in a) has the sequence of SEQ ID NO:201.

9. The isolated polynucleotide of claim 1, wherein the sequence encoding the polypeptide in a) has the coding sequence of SEQ ID NO:201.

10. A genetic construct comprising the polynucleotide of claim 1.

11. A host cell comprising a genetic construct of claim 10.

12. The host cell of claim 11 genetically modified to express a polynucleotide comprising a sequence encoding a polypeptide with at least 95% identity to the amino acid sequence of SEQ ID NO:163.

13. A plant cell or plant comprising the genetic construct of claim 10, or a plant part, propagule, progeny or see of the plant, wherein the plant part, propagule, progeny or seed of the plant comprises the genetic construct.

14. A plant cell or plant of claim 13 genetically modified to express a polynucleotide same as claim 13, or a plant part, propagule, progeny or see of the plant, wherein the plant part, propagule, progeny or seed of the plant expresses the polynucleotide.

15. An isolated polypeptide comprising:a) a sequence with at least 95% identity to the amino acid sequence of SEQ ID NO: 163; orb) a fragment of at least 5 contiguous amino acids of the sequence in a), wherein the fragment has essentially the same activity as the polypeptide in a).

16. The isolated polypeptide of claim 15, wherein the sequence in a) has the sequence of SEQ ID NO: 163.

17. An antibody raised against a polypeptide of claim 16.

18. An isolated polynucleotide encoding a polypeptide consisting of:a) a sequence with at least 95% identity to the amino acid sequence of SEQ ID NO: 163; orb) a fragment of at least 5 contiguous amino acids of the sequence in a).

19. A genetic construct comprising the polynucleotide of claim 18.

20. A probe or primer capable of hybridizing under stringent hybridization conditions to a polynucleotide consisting of:a) a sequence encoding a polypeptide with at least 95% identity to the amino acid sequence of SEQ ID NO:163; orb) the complement of the sequence of a).

21. The probe or primer of claim 20, comprising at least 15 contiguous nucleotides of a polynucleotide consisting of:a) a sequence encoding a polypeptide with at least 95% identity to the amino acid sequence of SEQ ID NO:163; orb) the complement of the sequence of a).

22. A method for producing a plant cell or plant with altered tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity; the method comprising the step of transformation of a plant cell or plant with a genetic construct including:a) at least one polynucleotide comprising:i) a sequence encoding a polypeptide with at least 95% identity to the amino acid sequence of SEQ ID NO:163; orii) the complement of the sequence of a); orb) at least one polynucleotide comprising a fragment, of at least 15 nucleotides in length, of the polynucleotide of a).

23. The method of claim 22, wherein the stress is environmental stress is drought stress.

24. A method for selecting a plant with altered tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity, the method comprising testing of a plant for altered expression of a polynucleotide comprising:a) a sequence encoding a polypeptide with at least 95% identity to the amino acid sequence of SEQ ID NO:163; orb) the complement of the sequence of a).

25. A method for selecting a plant with altered tolerance to at least one environmental stress selected from drought, cold, freezing, heat and salinity, the method comprising testing of a plant for altered expression of a polypeptide comprising:a) a sequence with at least 95% identity to the amino acid sequence of SEQ ID NO: 163.

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