Methods for identifying genomic deletions

Abstract

ible for Van Buchem's disease is narrowed to an approximately 92 kb region of human chromosome 17 at 17q21. Individuals afflicted with or carriers of Van Buchem's disease exhibit a 52 kb deletion within this 92 kb region. Methods are provided that permit the differentiation between individuals homozygous for and therefore afflicted with Van Buchem's disease, individuals heterozygous for and therefore carriers of Van Buchem's disease, and individuals who are normal with respect to Van Buchem's disease. Also provided are general methodologies for the detection of a wide variety of large genomic deletions.

Description

CROSS-REFERENCES TO RELATED APPLICATIONS

[0001]This application is a Continuation Under 35 U.S.C § 365 of co-pending International Application PCT/US01/23968, filed Jul. 30, 2001, which designates the U.S., and which claims priority to U.S. Provisional Patent Application No. 60/303,386, filed Jul. 6, 2001, and U.S. Provisional Patent Application No. 60/221,855, filed Jul. 28, 2000, which are incorporated herein by reference in their entirety.

BACKGROUND OF THE INVENTION

[0002]1. Field of the Invention

[0003]This invention relates generally to disease diagnosis and to the identification of disease carriers. More specifically, the present invention provides methods for identifying individuals who are afflicted with or carriers of diseases associated with one or more genomic deletion.

[0004]2. Description of the Related Art

[0005]Van Buchem's disease (VBD) is a rare autosomal recessive disorder that results in a bone dysplasia referred to as craniotubular hyperostosis. VBD was first described in 1962 as including osteosclerosis of the skull, mandible, clavicles, ribs, and diaphysis of the long bones beginning during puberty and, in some cases, leading to optic atrophy and perceptive deafness from nerve pressure. Van Buchem et al., Am. J. Med. 33:387-397 (1962).

[0006]More recently, additional occurrences of VBD have been reported. In 1988, Fryns et al. described a 7.5-year-old boy with VBD. This patient had presented at 2 months of age with left-side peripheral facial nerve palsy but had, at that time, no radiologically visible signs of sclerosis of the skull. Europ. J. Pediat. 147:99-100 (1988).

[0007]In 1997, Balemans et al. studied II VBD patients from a highly inbred and geophraphically isolated Dutch family. Each of these patients shared a common ancestor from 9 preceding generations. By applying a genome wide search for linkage using more than 300 microsatellite markers having an average spacing of 10 cM, these authors found a maximum lod score of 9.33 at theta=0.01 with marker D17S1299 and narrowed the assignment to a region of less than 1 cM between markers D17S1787 and D17S934. Am. J. Hum. Genet. 61(Suppl.):A12 (1997); See, also, Van Hul et al., Am. J. Hum. Genet. 62:391-399 (1998).

[0008]A related disease sclerosteosis is an autosomal semi-dominant disease that shares some of the clinical symptoms of VBD. The term "sclerosteosis" has been applied to a disorder similar to Van Buchem hyperostosis corticalis generalisata but differing in the radiologic appearance of the bone changes and in the presence of asymmetric cutaneous syndactyly of the index and middle fingers in many cases. In Handbuch der Kinderheilkunde 351-355 (Opitz, H. et al., Berlin: Springer (pub.), 1967). More specifically, this disease resembles VBD in that it comprises a progressive sclerosing bone dysplasia characterized by generalized osteosclerosis and hyperostosis of the skeleton, affecting mainly the skull and mandible thereby causing facial paralysis and hearing loss. In contrast to VBD, however, sclerosteosis is further characterized by gigantism and hand abnormalities.

[0009]The rare genetic mutation responsible for the sclerosteosis syndrome has been localized to the region of human chromosome 17 that encodes a novel member of the TGF-beta binding-protein family (one representative example of which is designated "hSOST"). In 1999, Balemans et al. assigned the locus for sclerosteosis to 17q12-q21 which is the same general region as the locus for VBD. Am. J. Hum. Genet. 64:1661-1669 (1999). Due to the clinical similarities between VBD and sclerosteosis, Beighton et al. suggested that these conditions might be caused by mutations within the same gene. Clin. Genet. 25:175-181 (1984). This hypothesis was further supported by the genetic experimentation later performed by Balemans et al. Supra.

[0010]Traditional methodologies for identifying genomic deletions such as, for example, restriction fragment length polymorphism (RFLP), fluorescence in situ hybridization (FISH) and Southern blotting permit the identification of individuals who are homozygous for a genomic deletion and, as a consequence, are afflicted with the associated genetic disease. Because these methods are time consuming and/or require high-quality DNA samples or live cells, they are of limited use in the identification of individuals who are heterozygous for and, therefore, carriers of a genetic disease. What is needed in the art are methods that permit the rapid identification of genetic disease carriers, which methods distinguish between individuals who are homozygous for a genomic deletion, individuals who are heterozygous for a genomic deletion and individuals who do not possess a given genomic deletion. As described in detail herein, the present invention fulfills this and other related needs.

BRIEF SUMMARY OF THE INVENTION

[0011]The present invention is directed generally to disease diagnosis and to the identification of disease carriers. More specifically, the invention disclosed herein provides methods for identifying chromosomal deletions that are associated with a disease phenotype. Particular methods within the scope of the present invention are directed to the identification of individuals who are afflicted with or carriers for the genomic deletion associated with Van Buchem's disease. By alternate embodiments, the present invention also provides methods having general utility in the detection of a wide variety of diseases characterized by genomic deletions.

[0012]The present invention provides, in one embodiment, methods for distinguishing between an individual who is homozygous for a genomic deletion, an individual who is heterozygous for a genomic deletion and an individual who is negative for a genomic deletion.

[0013]A first method comprises: (a) obtaining a sample of genomic DNA from an individual; (b) performing a first amplification reaction with a first oligonucleotide primer pair comprising a first oligonucleotide primer and a second oligonucleotide primer wherein the first oligonucleotide primer is complementary to the nucleotide sequence upstream of the genomic deletion and the second oligonucleotide primer is complementary to the nucleotide sequence downstream of said genomic deletion; (c) performing a second amplification reaction with a second oligonucleotide primer pair comprising a third oligonucleotide primer and a fourth oligonucleotide primer wherein the third oligonucleotide primer is complementary to the nucleotide sequence either upstream or downstream of the genomic deletion and the fourth oligonucleotide primer is complementary to the nucleotide sequence comprising the genomic deletion; and (d) detecting the product of the amplification reactions of (b) and (c).

[0014]By this first type method, a positive amplification reaction of (b) and a negative amplification reaction of (c) indicates an individual who is homozygous for the large genomic deletion; a positive amplification reaction of (b) and a positive amplification reaction of (c) indicates an individual who is heterozygous for the large genomic deletion; and a negative amplification reaction of (b) and a positive amplification reaction of (c) indicates an individual who is negative for the large genomic deletion.

[0015]A second method comprises: (a) obtaining a sample of genomic DNA from said individual; (b) performing an amplification reaction employing at least two oligonucleotide primer pairs in which an oligonucleotide primer is common to both said primer pairs, wherein a first primer pair has a first oligonucleotide primer complementary to a nucleotide sequence that flanks said genomic deletion upstream of said genomic deletion and a second oligonucleotide primer complementary to a nucleotide sequence that flanks said genomic deletion downstream of said genomic deletion, and a second primer pair has third oligonucleotide primer complementary to a nucleotide sequence within said genomic deletion and either said first or second oligonucleotide primer; and (c) detecting an amplified product of said amplification reaction.

[0016]By this second method, a positive amplification reaction of said first primer pair and a negative amplification reaction of said second primer pair indicates an individual that is homozygous for said large genomic deletion; a positive amplification reaction of said first primer and a positive amplification reaction of said second primer pair indicates an individual that is heterozygous for said large genomic deletion; and a negative amplification reaction of said first primer pair and a positive amplification reaction of said second primer pair indicates an individual that is negative for said large genomic deletion.

[0017]Both these methodologies will find utility in the detection of large genomic deletions comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40 or 50 kb. By some embodiments, the presence of the large genomic deletion is indicative of an individual who is either afflicted with or a carrier of a genetic disease. The genetic disease is exemplified herein by Van Buchem's disease, but the methods are suitable for any disease characterized by large genomic deletions.

[0018]In further embodiments, the present invention provides a first method for identifying individuals who are afflicted with or carriers of Van Buchem's disease. This first method comprises (a) obtaining a sample of genomic DNA from an individual; (b) performing a first amplification reaction with a first oligonucleotide pair primer having a first oligonucleotide primer and a second oligonucleotide primer wherein the first oligonucleotide primer is complementary to the nucleotide sequence upstream of the 51,719 bp sequence depicted in SEQ ID NO:2 which sequence corresponds to nucleotides 5,798 through 57,516 of the 92,149 bp nucleic acid sequence depicted in SEQ ID NO:1 and the second oligonucleotide primer is complementary to the nucleotide sequence downstream of the 51,719 bp sequence depicted in SEQ ID NO:2; and (c) performing a second amplification reaction with a second oligonucleotide pair primer comprising a third oligonucleotide primer and a fourth oligonucleotide primer wherein the third oligonucleotide primer is complementary to the nucleotide sequence either upstream or downstream of the 51,719 bp sequence depicted in SEQ ID NO:2 and the fourth oligonucleotide is complementary to the nucleotide sequence within the 51,719 bp sequence depicted in SEQ ID NO:2; and (d) detecting the product of the amplification reactions of (b) and (c).

[0019]By this first method, a positive amplification reaction of (b) and a negative amplification reaction of (c) indicates an individual afflicted with Van Buchem's disease; a positive amplification reaction of (b) and a positive amplification reaction of (c) indicates an individual who is a carrier of Van Buchem's disease; and a negative amplification reaction of (b) and a positive amplification reaction of (c) indicates an individual that is neither afflicted with nor a carrier of Van Buchem's disease.

[0020]Exemplary first oligonucleotide primer pairs may be selected from the group consisting of 12952/VBspan1 (SEQ ID NO:84/SEQ ID NO:85), Span1F/Span1R (SEQ ID NO:86/SEQ ID NO:87), Span2F/Span2R (SEQ ID NO:88/SEQ ID NO:89) and Vbspan2/Vbspan1 (SEQ ID NO:104/SEQ ID NO:85). Exemplary second oligonucleotide pairs may be selected from the group consisting of 12952/Wt1R (SEQ ID NO:84/SEQ ID NO:90), Wt2F/Wt2R (SEQ ID NO:91/SEQ ID NO:92), Wt3F/Wt3R (SEQ ID NO:93/SEQ ID. NO:94) and VBspan2/VBint1 (SEQ ID NO:105/SEQ ID NO:102).

[0021]A second method comprises: (a) obtaining a sample of genomic DNA from said individual; (b) performing a polymerase chain reaction employing at least two oligonucleotide primer pairs in which an oligonucleotide primer is common to both said primer pairs, wherein a first primer pair has a first oligonucleotide primer that is complementary to a nucleotide sequence upstream of the 51,719 bp sequence provided in SEQ ID NO:2 and a second oligonucleotide primer that is complementary to a nucleotide sequence downstream of the 51,719 bp sequence provided in SEQ ID NO:2, and a second primer pair has a third oligonucleotide primer that is complementary to a nucleotide sequence within said genomic deletion and either said first or second oligonucleotide primer; and (c) detecting an amplified product of said amplification reaction.

[0022]By this second method, a positive polymerase chain reaction of said first primer pair and a negative polymerase chain reaction of said second primer pair indicates an individual afflicted with Van Buchem's disease; a positive polymerase chain reaction of said first primer pair and a positive polymerase chain reaction of said second primer pair indicates an individual that is a carrier of Van Buchem's disease; and a negative polymerase chain reaction of said first primer pair and a positive polymerase chain reaction of said second primer pair indicates an individual that is neither afflicted with nor a carrier of Van Buchem's disease.

[0023]Exemplary first oligonucleotide primer pair is selected from the group consisting of 12952/VBspan1 (SEQ ID NO:84/SEQ ID NO:85), Span1F/Span1R (SEQ ID NO:86/SEQ ID NO:87), Span2F/Span2R (SEQ. ID NO:88/SEQ ID NO:89), Wt2F/VBspan1 (SEQ ID NO:91/SEQ ID NO:85) and VBspan2/VBspan1 (SEQ ID NO:104/SEQ ID NO:85).

[0024]Still further embodiments of the present invention provide alternative methods for identifying individuals afflicted with Van Buchem's disease. Exemplary methods comprise the steps of performing an amplification reaction with a pair of oligonucleotides selected from the region between nucleotide 1 and nucleotide 51,719 of SEQ ID NO:2 wherein the absence of an amplification product indicates an individual homozygous for Van Buchem's disease. Exemplary oligonucleotide pairs are selected from the group consisting of Del1F/Del1R (SEQ ID NO:95/SEQ ID NO:96), Del2F/Del2R (SEQ ID NO:97/SEQ ID NO:98), and Del3F/Del3R (SEQ ID NO:99/SEQ ID NO:100).

[0025]Other embodiments provide methods for identifying an individual who is homozygous for Van Buchem's disease comprising the step of detecting a deletion in human chromosome 17 at 17q21 between nucleotide 5,798 and nucleotide 57,516 as depicted in SEQ ID NO:1.

[0026]By still other embodiments are provided methods for detecting an individual who is afflicted with or a carrier of Van Buchem's disease which methods comprise detecting the nucleotide sequence spanning the deletion breakpoint as depicted in FIG. 2 and SEQ ID NO:101. By these methods, the presence of a deletion breakpoint indicates an individual who is either afflicted with or a carrier of Van Buchem's disease. Exemplary nucleotide sequences spanning the deletion breakpoint comprise the nucleotide sequence 5'-ACCATGCCCGGCTAAT-3' (SEQ ID NO:102); the nucleotide sequence 5'-CTACCATGCCCGGCTAATTIT-3' (SEQ ID NO:103); and the nucleotide sequence 5'-TGGGATTACAGGTGCATGCTACCATGCCCGGCTAATTTTTTTGTA TTTTTTTAGTA-3' (SEQ ID NO:101).

[0027]In still further embodiments of the present invention are provided isolated polynucleotides. Preferred polynucleotides comprise at least 10, 15, 20, 25, 30, 40, 50, 100, 250 or 500 contiguous nucleotides of the nucleic acid depicted in SEQ ID NO:1. Alternatively, isolated polynucleotides according to the present invention hybridize under moderately stringent conditions to the nucleic acid depicted in SEQ ID NO:1 or the complement thereof. Preferred moderately stringent conditions comprise 2×SSC, 0.1% SDS at 65° C.

[0028]Other embodiments provide isolated polynucleotides that comprise at least 10, 15, 20, 25, 30, 40, 50, 100, 250 or 500 nucleotides of one of the amplicons comprising the predicted exons within human chromosome 17 at 17q21 which amplicons are selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, and SEQ ID NO:17.

[0029]Still further embodiments of the present invention provide diagnostic kits for distinguishing between an individual who is homozygous for a large genomic deletion, an individual who is heterozygous for a large genomic deletion and an individual who is negative for a large genomic deletion.

[0030]A first kit comprises: (a) a first oligonucleotide primer pair comprising a first oligonucleotide primer and a second oligonucleotide primer, wherein said first oligonucleotide primer is complimentary to a nucleotide sequence upstream of said genomic deletion and said second oligonucleotide primer is complementary to a nucleotide sequence downstream of said genomic deletion; and (b) a second oligonucleotide primer pair comprising a third oligonucleotide primer and a fourth oligonucleotide primer wherein said third oligonucleotide primer is complementary to a nucleotide sequence either upstream or downstream of said genomic deletion and said fourth oligonucleotide primer is complementary to a nucleotide sequence within said genomic deletion. These types of diagnostic kit can further comprises instructions for distinguishing between an individual who is homozygous for a large genomic deletion, an individual who is heterozygous for a large genomic deletion and an individual who is negative for a large genomic deletion.

[0031]A second kit comprises: (a) a first primer pair having a first oligonucleotide primer that is complementary to a nucleotide sequence that flanks said genomic deletion upstream of said genomic deletion and a second oligonucleotide primer that is complementary to a nucleotide sequence that flanks said genomic deletion downstream of said genomic deletion; and (b) a second primer pair having a third oligonucleotide primer that is complementary to a nucleotide sequence within said genomic deletion and either said first or second oligonucleotide primer.

[0032]The genomic deletion mentioned can be associated with Van Buchem's disease. In which case one type of diagnostic kit for identifying a carrier of Van Buchem's disease comprises: (a) a first oligonucleotide primer pair comprising a first oligonucleotide and a second oligonucleotide wherein said first oligonucleotide is complementary to a nucleotide sequence upstream of the 51,719 bp sequence depicted in SEQ ID NO:2 and said second oligonucleotide is complementary to a nucleotide sequence downstream of the 51,719 bp sequence depicted in SEQ ID NO:2; and (b) a second oligonucleotide primer pair comprising a third oligonucleotide and a fourth oligonucleotide wherein said third oligonucleotide is complementary to either the nucleotide sequence upstream or downstream of said 51,719 bp sequence depicted in SEQ ID NO:2 and said fourth oligonucleotide is complementary to a nucleotide sequence within said 51,719 bp sequence depicted in SEQ ID NO:2. These diagnostic kits can further comprise instructions for identifying a carrier of Van Buchem's disease.

[0033]A second kit comprises: (a) a first oligonucleotide pair having a first oligonucleotide primer that is complementary to a nucleotide sequence upstream of the 51,719 bp sequence provided in SEQ ID NO:2 and a second oligonucleotide primer that is complementary to a nucleotide sequence downstream of the 51,719 bp sequence depicted in SEQ ID NO:2; and (b) a second oligonucleotide pair having a third oligonucleotide primer that is complementary to a nucleotide sequence within said 51,719 bp sequence provided in SEQ ID NO:2 and either said first or second oligonucleotide primer.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF DRAWINGS

[0034]FIG. 1A illustrates results obtained from microsatellite typing and exon sequencing of the approximately 90 kb region between the MEOX1 and SOST genes within human chromosome region 17q21. FIG. 1B shows the about 53 bp genomic deletion as well as illustrates the nucleotide sequence spanning the deletion breakpoint (SEQ ID NO:101) in nucleic acid sequences obtained from individuals afflicted with Van Buchem's disease.

[0035]FIG. 2 illustrates expected results from the polymerase chain reactions utilizing exemplary oligonucleotide primer pairs in the first-described method, where the nucleic acid sample is obtained from an individual who is (1) a non-carrier for Van Buchem's disease; (2) heterozygous for, and therefore a carrier of, Van Buchem's disease; or (3) homozygous for, and therefore afflicted with, Van Buchem's disease.

[0036]FIG. 3 illustrates expected results from the polymerase chain reaction utilizing exemplary oligonucleotide primer pairs in the second-described method, where the nucleic acid sample is obtained from an individual who is (1) a non-carrier for Van Buchem's disease; (2) heterozygous for, and therefore a carrier of, Van Buchem's disease; or (3) homozygous for, and therefore afflicted with, Van Buchem's disease.

DETAILED DESCRIPTION OF THE INVENTION

[0037]As part of the present invention, it was discovered, through haplotype analysis of a number of VBD patients carrying recombinant chromosomes, that the VBD gene localized to a critical region of less than 1 Mb, between the polymorphic markers BP12574 (D17S2250) and JM6307 (D17S2253) (see Example 1). Further microsatellite typing, as well as characterization of the candidate genes within the 1 Mb region through exon sequencing, revealed a specific chromosomal aberration that segregated absolutely with VBD (see Example 3).

[0038]As discussed in detail in Example 3, two independent experimental approaches to identifying the VBD candidate region revealed the presence of a chromosomal deletion associated with VBD. First, the oligonucleotide pair D17S1789 (see FIG. 1 and Table 3) did not permit the PCR amplification of a DNA fragment from genomic DNA isolated from VBD patients but did facilitate the amplification of a fragment from normal individuals. Second, nucleotide sequencing and computational analysis, with the GENSCAN exon prediction algorithm of Burge et al., of a 92,149 bp fragment (SEQ ID NO:1) within the human 17q21 chromosomal locus revealed the presence of 15 putative exon sequences. J. Mol. Biol. 268:78-94 (1997). As presented in FIG. 1 and as discussed in detail in Example 3, 12 of these 15 exon fragments did not amplify from genomic DNA isolated from VBD-afflicted individuals.

[0039]In total, these results demonstrated for the first time the presence of a genomic deletion within human chromosome 17q21 that segregates absolutely with Van Buchem's disease. Furthermore, nucleotide sequencing of the region spanning the deletion breakpoint (SEQ ID NO:101) revealed that approximately 52 kb of contiguous genomic DNA is invariably deleted from chromosome 17 at position 17q21 in individuals afflicted with VBD. The sequence of the 52 kb region is presented herein as SEQ ID NO:2.

Isolated Polynucleotides

[0040]As noted above, the present invention provides isolated polynucleotides comprising at least 10, 15, 20, 25, 30, 40, 50, 100, 250, 500, 600, 700, 800 up to and including the 92,101 base pairs (bp) nucleotide sequence of human chromosome 17 at 17q21 disclosed herein as SEQ ID NO:1. Also provided by the present invention are isolated polynucleotides comprising at least 10, 15, 20, 25, 30, 40, 50, 100, 250, 500 bp up to and including the 52 kb sequence of 17q21 that is deleted in individuals who are either afflicted with or carriers of Van Buchem's disease (i.e. SEQ ID NO:2). As well, the present invention provides polynucleotides comprising at least 10, 15, 20, 25, 30, 40, 50, 100, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850 or 890 bp of any of the exons depicted in SEQ ID NOs: 3-17. Other embodiments of the present invention provide oligonucleotide probes of at least 10, 15, 20, 25, 30, 40, 50, 100, 250, 500, 600, 750, 1000, 1050, 1100, 1200 or 1300 that hybridize under moderately stringent conditions to the nucleotide sequence of SEQ ID NO:1 or complements thereto. Exemplary oligonucleotide probes according to the present invention include those designated herein by SEQ ID NOs:28-100.

[0041]As used herein, the phrase "isolated polynucleotide" refers to a DNA molecule that has been isolated free of total genomic DNA of a particular species. As will be understood by those skilled in the art, the isolated polynucleotides of the present invention can include genomic sequences, extra-genomic and plasmid-encoded sequences and smaller engineered gene segments that express, or may be adapted to express, proteins, polypeptides, peptides and the like. Such segments may be naturally isolated, or synthesized de novo.

[0042]Thus, "isolated," as used herein, means that the polynucleotide is substantially separated away from other genomic sequences and that the polynucleotide does not contain large portions of unrelated coding DNA, such as large chromosomal fragments. Of course, this refers to the polynucleotide as originally isolated, and does not exclude genes or coding regions later added to the segment.

[0043]As will be recognized by the skilled artisan, isolated polynucleotides may be single-stranded or double-stranded, and may be DNA or RNA molecules. Additional coding or non-coding sequences may, but need not, be present within a polynucleotide of the present invention, and a polynucleotide may, but need not, be linked to other molecules and/or support materials.

[0044]It is also contemplated that the present invention encompasses polynucleotides having substantial identity to any of the sequences disclosed herein, for example the present invention encompasses those isolated polynucleotides comprising at least 50% or more sequence identity, preferably at least 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% or higher, sequence identity compared to a polynucleotide sequence of this invention using the methods described herein, (e.g., BLAST, described in Altschul et al., J. Mol. Biol. 215:403-410 (1990)). For the purposes of this invention, a preferred method of calculating percent identity is the Smith-Waterman algorithm, using the following. Global DNA sequence identity must be at least 50% as determined by the Smith-Waterman homology search algorithm as implemented in MPSRCH program (Oxford Molecular) using an affine gap search with the following search parameters: gap open penalty, 12; and gap extension penalty, 1.

[0045]In additional embodiments, the present invention provides isolated polynucleotides comprising various lengths of contiguous stretches of sequence identical to or complementary to one or more of the sequences disclosed herein. For example, isolated polynucleotides are provided by this invention that comprise at least about 10, 15, 20, 25, 30, 40, 50, 100, 250, 500, 600, 750, 1000, 1050, 1100, 1200 or 1300 or more contiguous nucleotides of one or more of the sequences disclosed herein.

[0046]The polynucleotides of the present invention, or fragments thereof, may be combined with other DNA sequences, such as promoters, polyadenylation signals, additional restriction enzyme sites, multiple cloning sites, other coding segments, and the like, such that their overall length may vary considerably. It is therefore contemplated that a nucleic acid fragment of almost any length may be employed, with the total length preferably being limited by the ease of preparation and use in the intended recombinant DNA protocol.

[0047]In other embodiments, the present invention is directed to polynucleotides that are capable of hybridizing under moderately stringent conditions to a polynucleotide sequence provided herein, or a fragment thereof, or a complementary sequence thereof. Hybridization techniques are well known in the art of molecular biology. For purposes of illustration, suitable moderately stringent conditions for testing the hybridization of a polynucleotide of this invention with other polynucleotides includes hybridization in a solution of 2×SSC, 0.1% SDS, 1.0 mM EDTA (pH 8.0) at 65° C.

[0048]In still other embodiments of the present invention, the polynucleotide sequences provided herein may be employed as primers in the amplification methodology disclosed herein, infra. As such, it is contemplated that polynucleotides comprising at least about 10 nucleotides that have the same sequence as, or that are complementary to, a 10 nucleotide long contiguous sequence disclosed herein will find utility as an amplification primer. Longer contiguous identical or complementary sequences, e.g., those of about 15, 20, 25, 30, 40, 50, 100, 250, 500, 600, 750, 1000, 1050, 1100, 1200 or 1300 or more will also be of use in certain embodiments.

[0049]Small polynucleotide segments or fragments may be readily prepared by, for example, directly synthesizing the fragment by chemical means, as is commonly practiced using an automated oligonucleotide synthesizer. Also, fragments may be obtained by application of nucleic acid reproduction technology, such as the PCR® technology of U.S. Pat. No. 4,683,202 (incorporated herein by reference), by introducing selected sequences into recombinant vectors for recombinant production, and by other recombinant DNA techniques generally known to those of skill in the art of molecular biology. See, e.g., Ausubel et al. (eds.), Short Protocols in Molecular Biology (3^rd Ed., John Wiley & Sons 1995).

Methodologies for Detecting Van Buchem's Disease Carriers

[0050]As noted above, the present invention provides methods for detecting carriers of Van Buchem's disease. More specifically, within certain embodiments, these methods permit the differentiation between individuals who are homozygous for, and therefore afflicted with, VBD; individuals who are heterozygous for, and therefore carriers of, VBD; and individuals who are normal with respect to VBD. As described in detail herein, infra, the presently disclosed methodology will find general utility in the detection of a wide variety of diseases associated with genomic deletions of any length.

[0051]The methodology according to the present invention relies, in part, on the inability of various DNA polymerases that are typically utilized in amplification reactions to amplify DNA fragments beyond a maximum nucleotide length. As discussed above, the genomic locus associated with Van Buchem's disease is associated with a large chromosomal deletion of approximately 52 kb at 17q21. Consequently, this deletion results in the juxtaposition of nucleotide sequences present in the genomic DNA of non-VBD-afflicted individuals that are normally separated by greater than 52 kb. Because DNA polymerases normally utilized for the amplification of DNA fragments cannot amplify a 52 kb fragment, a pair of oligonucleotide primers that bind to nucleotide sequences upstream or downstream of this 52 kb region will not permit the amplification of DNA from a non-VBD-afflicted individual. In contrast, however, the same pair of oligonucleotide primers will facilitate the amplification of a DNA fragment from genomic DNA isolated from either a VBD-afflicted individual or from a VBD carrier.

[0052]The figures illustrate the genomic DNA corresponding to human chromosome 17 at 17q21 between the MEOX1 gene and exon 4. The genomic DNA from non-VBD-afflicted individuals comprises a 52 kb fragment described above while genomic DNA from VBD-afflicted individuals lack this 52 kb region. FIGS. 2 and 3 also illustrate exemplary oligonucleotide primers that hybridize to various regions within the 7q21 locus. Oligonucleotide primers designated VBspan2 and VBspan1/12953, and 12952 and VBspan1/12953 hybridize to nucleotide sequences, on complementary strands, that flank this 52 kb region in normal genomic DNA and, consequently, that flank the deletion breakpoint in genomic DNA from VBD-afflicted individuals. Because of the intervening 52 kb fragment in normal genomic DNA, neither the VBspan2/VBspan1 oligonucleotide primer pair nor the 12952/12953 oligonucleotide primer pair will permit the amplification (e.g., by PCR; see infra) of a DNA fragment from non-VBD-afflicted individuals. In contrast, the VBspan2/VBspan1 oligonucleotide pair primer will facilitate the amplification of a 642 bp fragment, and the 12952/12953 oligonucleotide pair primer will facilitate the amplification of a 798 bp fragment from the DNA of a VBD-afflicted individual.

[0053]The methodology according to the present invention additionally permits the differentiation between an individual who is heterozygous for the 52 kb genomic deletion and therefore a carrier of VBD; an individual who is homozygous for the 52 kb genomic deletion and therefore afflicted with VBD; and an individual who is neither a carrier of nor afflicted with VBD. This is achieved by the performance of a second amplification reaction employing a second pair of oligonucleotide primers wherein a third oligonucleotide primer hybridizes to a region of the genomic DNA flanking, either upstream or downstream of, the 52 kb sequence and a fourth oligonucleotide primer hybridizes to a region of the genomic DNA within the 52 kb sequence.

[0054]Alternatively, this can be performed using a single multiplex reaction employing a second pair of oligonucleotide primers wherein a third oligonucleotide primer hybridizes to a region of the genomic DNA within the 52 kb sequence and the other oligonucleotide primer is one of the primers from the first oligonucleotide primer pair. Such an oligonucleotide pair is exemplified in FIG. 3 by the VBspan2/VBint1 (SEQ ID NO:104/SEQ ID NO:105) oligonucleotide pair (SEQ ID NO:91/SEQ ID NO:92). As depicted in FIG. 3, a PCR reaction employing this oligonucleotide pair will amplify a 720 bp DNA fragment from the genomic DNA from an individual who is neither afflicted nor a VBD-carrier and from the genomic DNA of a heterozygous VBD-carrier but will not amplify a DNA fragment from the genomic DNA of a homozygous VBD-afflicted individual.

[0055]The methodologies according to the present invention permit an alternative approach to differentiating between an individual who is heterozygous for the 52 kb genomic deletion and therefore a carrier of VBD; an individual who is homozygous for the 52 kb genomic deletion and therefore afflicted with VBD; and an individual who is neither a carrier of nor afflicted with VBD, as described in Examples 4 and 5.

[0056]In Example 4, this can be achieved using at least two amplification reactions. In Example 5, this can be achieved by the performance of a single multiplex amplification reaction employing at least two oligonucleotide primer pairs, in which one oligonucleotide is common to both pairs. Thus the amplification reaction includes three oligonucleotide primers in total.

[0057]Thus, by the present invention provides two methodologies that permit the identification of VBD carriers by allowing the differentiation between an individual who is homozygous for the 52 kb genomic deletion and, therefore, afflicted with VBD; an individual who is heterozygous for the 52 kb genomic deletion and, therefore, a carrier for VBD; and an individual who is normal with respect to the 52 kb deletion and, therefore, neither a carrier of nor afflicted with VBD.

[0058]While the present invention discloses the nucleotide sequences of specific exemplary oligonucleotide primers that may be utilized according to the methodologies disclosed herein, it will be apparent to the skilled artisan that alternative oligonucleotide primers may be designed based on the nucleotide sequence depicted in SEQ ID NO:1 which primers are entirely within the scope of the present invention. Thus, for example, suitable oligonucleotide primers according to the present invention include polynucleotides comprising at least 10, 15, 20, 25, 30, 40, 50, 60, 75, 90 or 100 contiguous nucleotides of the nucleic acid depicted in SEQ ID NO:1.

[0059]It will also be apparent to one skilled in the art that the choice of reaction conditions and DNA polymerases employed for performing the amplification reactions may be varied in accordance with such parameters as the nucleotide sequence of the oligonucleotide primers and the distance between the primer pairs. Exemplary conditions for 25 ul reactions according to the present methodologies include, but are not limited to, the following: 10 ng genomic DNA; 60 mM TrisHCl pH 7.5; 15 mM ammonium sulfate; 2.5 mM magnesium chloride; 0.4 mM dNTP; and 0.2 uM of each oligonucleotide primer. A typical amplification protocol involves an initial denaturation at 94° C. for 3 min.; 40 cycles of 94° C. for 1 min., 68° C. for 1 min. and 72° C. for 1 min.; and a final extension reaction at 72° C. for 5 min. Amplicons may be resolved by electrophoresis on a 1% agarose gel run in 1×TAE buffer and may be detected by staining with ethidium bromide. Further exemplary conditions for 50 μl reactions according to the present methodologies include, but are not limited to, the following: 20 ng genomic DNA, 0.4 μM each of VBspan1 and VBint1, 0.8 μM of VBspan2, 0.2 mM of each dNTP, 120 mM TrisHCl, pH 7.5, 30 mM ammonium sulfate, 5.0 mM magnesium chloride and 2.5 U Taq polymerase (Roche Molecular Biochemicals, Indianapolis, Ind.). A further typical amplification protocol involves an initial denaturation at 94° C. for 3 min, 40 cycles of 94° C. for 30 sec, 63° C. for 30 sec, 72° C. for 2 min and a final extension of 72° C. for 5 min. Amplicons may be resolved by electrophoresis on a 2.5% agarose gel run in 1×TAE buffer and may be detected by staining with ethidium bromide.

[0060]Genomic DNA may be isolated by standard procedures that are readily available to those of skill in the art. Representative methodology are provided, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratories, Cold Spring Harbor, N.Y., 1989) and in Ausubel et al. (eds.), Short Protocols in Molecular Biology (4^th Ed., John Wiley & Sons 1999).

[0061]General methodology for performing the polymerase chain reaction (PCR®) are described in detail in U.S. Pat. Nos. 4,683,195; 4,683,202 and 4,800,159, each of which is incorporated herein by reference in its entirety. Briefly, in PCR®, two primers are prepared which have sequences that are complementary to regions on opposite complementary strands of the target sequence. An excess of deoxynucleoside triphosphates is added to a reaction mixture along with a DNA polymerase (e.g., Taq polymerase; see, infra, for exemplary alternative polymerases). If the target sequence is present in a sample, the primers will bind to the target and the polymerase will cause the primers to be extended along the target sequence by adding on nucleotides. By raising and lowering the temperature of the reaction mixture, as indicated, supra, the extended primers will dissociate from the target to form reaction products, excess primers will bind to the target and to the reaction product and the process is repeated.

[0062]In addition to the polymerase chain reaction, it is contemplated that alternative amplification procedures that are readily available in the art may be employed in the methodology disclosed herein. For example, another suitable method for amplification is the ligase chain reaction (LCR) and is disclosed in Eur. Pat. Appl. Publ. No. 320,308: (incorporated herein by reference). Briefly, in LCR, two complementary probe pairs are prepared, and in the presence of the target sequence, each pair will bind to opposite complementary strands of the target such that they abut. In the presence of a ligase, the two probe pairs will link to form a single unit. By temperature cycling, as in PCR®, bound ligated units dissociate from the target and then serve as "target sequences" for ligation of excess probe pairs. For a related technique, see also, U.S. Pat. No. 4,883,750, incorporated herein by reference in its entirety.

[0063]Alternatively, other methods may be similarly employed in place of the polymerase chain reaction for amplification of nucleic acid sequences include the following: (1) Qbeta Replicase, PCT Intl. Pat. Appl. Publ. No. PCT/US87/00880; (2) the isothermal amplification method of Walker et al.; (3) strand displacement amplification (SDA); (4) repair chain reaction (RCR); (5) cyclic probe reaction (CPR); see, also, Great Britain Pat. Appl. No. 2 202 328, and in PCT Intl. Pat. Appl. Publ. No. PCT/US89/01025; (7) transcription-based amplification systems (TAS) Kwoh et al., 1989; PCT Intl. Pat. Appl. Publ. No. WO 88/10315; (8) Eur. Pat. Appl. Publ. No. 329,822; (9) PCT Intl. Pat. Appl. Publ. No. WO 89/06700; (10) "RACE" (Frohman, 1990); (11) "one-sided PCR" (Ohara, 1989). Each of these methods is incorporated herein by reference.

[0064]The choice of DNA polymerase to be employed in the polymerase chain or other amplification reaction can also be determined by the skilled artisan without undue experimentation. As discussed above, the major consideration in selecting an appropriate polymerase is the maximum length of DNA fragment that may be amplified. Such a determination may be achieved initially by reference to the guidelines set forth by the individual manufacturers and ultimately through empirical means by testing stepwise increases in the length of DNA fragment amplified. Additionally or alternatively, the precise reaction condition parameters such as, for example, salt concentration, divalent cation employed and reaction temperature may all be varied in order to adjust, as desired, the length of DNA fragment that may be amplified. The determination of optimal reaction parameters is wholly within the expertise of the skilled artisan.

[0065]Exemplary polymerases that may be employed in the present methodology include the various DNA polymerases, and variants thereof, isolated from thermostable bacteria such as the following: Taq, Vent and Deep Vent DNA polymerases (New England BioLabs; Beverly, Mass.); AdvanTaq®, AdvanTaq Plus®, and Advantage® 2 (Clontech; Palo Alto, Calif.); SuperTaq (Ambion; Austin, Tex.); Tth and Pwo polymerases (Roche Molecular Biochemicals; Indianapolis, Ind.); AmpliTaq (PE Biosystems; Foster City, Calif.); and Taq2000 and Pfu DNA polymerases (Stratagene; La Jolla, Calif.).

General Methods for Detection of Large Genomic Deletions

[0066]The methodology disclosed herein, supra, for the detection of the large genomic deletion associated with Van Buchem's disease and carriers thereof may be applied broadly, and therefore will find general utility, in the detection of a wide variety of diseases associated with large genomic deletions. In short, the present methods take advantage of the inability of commonly available DNA polymerases to amplify nucleic acid fragments above a certain maximum size when employed in a polymerase chain or other amplification reaction.

[0067]Thus, as noted above, the present invention provides general methodologies for distinguishing between an individual who is homozygous for a large genomic deletion, an individual who is heterozygous for a large genomic deletion and an individual who is negative for a large genomic deletion. These methods are discussed in detail in Examples 4 and 5.

[0068]As used herein, the phrase "large genomic deletion" refers to those deletions that result from the loss of a contiguous stretch of genomic DNA of such a length that it cannot be amplified in an amplification reaction as disclosed herein, supra. One skilled in the art will recognize, therefore, that the phrase "large genomic deletion" will vary depending on the precise reaction conditions and DNA polymerases employed in the amplification reactions. Generally, the phrase "large genomic deletion" may refer to deletions of at least 2 contiguous kilobases (kb) of genomic DNA. Alternatively, a "large genomic deletion" refers to deletions of at least 3, 4, 5, 6, 7, 8, 9, 10 or more contiguous kb of genomic DNA. Also included within the definition of "large genomic deletions" are deletions of at least 15, 20, 25, 30, 40 or 50 contiguous kb of genomic DNA.

EXAMPLES

[0069]The following experimental examples are offered by way of illustration, not limitation.

Example 1

The Van Buchem's Disease Locus Localizes to a Region of Less than 1 Mb

[0070]This example discloses that haplotype analysis of a number of Van Buchem's Disease patients carrying recombinant chromosomes localized the disease to a critical region of less than 1 Mb in a region between the polymorphic markers BP12574 (D17S2250) and JM6307 (D17S2253).

[0071]The Van Buchem's Disease region was previously localized to the ˜1 cM interval between polymorphic markers D17S1787 and D17S1861 on chromosome 17q12-21. To refine the interval further, genomic DNA samples were obtained from 15 affected individuals, 4 of whom had not been previously analyzed. A number of microsatellite markers from chromosome 17q12-21 were used to analyze the DNA samples. Patient DNA samples were genotyped using PCR amplification of polymorphic microsatellite markers. The microsatellite markers selected (see Table 1) are described in public databases or were developed using the SPUTNIK algorithm on sequence obtained from a BAC contig across the van Buchem disease/sclerosteosis region (Brunkow et al., Am. J. Hum. Genet. 68:577-584 (2001)), D17S1787 to D17S1861. PCR products were labeled with infrared IRDyes® using an M13 tailing approach as described in Oetting et al., Genomics 30:450-458 (1995) and were resolved on a LiCor 4000 DNA sequencer. Allele determinations were made using the SAGA genotyping analysis software (University of Washington) or by a trained eye.

[0072]Using this large set of novel polymorphic markers derived from a BAC contig across the D17S1787 and D17S1861 interval, a large region of homozygosity across the disease locus was observed. Furthermore, all affected individuals (including 12-15) shared a common disease haplotype (Table 1). Individuals 2, 3, 6-8 and 12-15 were nonrecombinant across the entire D17S1787-D17S1861 interval, while individuals 1, 4, 5 and 9, 10, 11 carried recombinant chromosomes. Accordingly, the disease locus was refined to the <1 Mb region between BP12574 (D17S2250) and JM6307 (D17S2253) (see Table 1).

TABLE-US-00001 TABLE 1 Marker genotypes of affected individuals for selected markers at the van Buchem disease locus on chromosome 17q12-q21 CONSENSUS CONSENSUS DUTCH VAN BUCHEM DISEASE GENOTYPES VBD SCLEROSTEOSIS AFFECTED INDIVIDUAL HAPLOTYPE HAPLOTYPE MARKER 1 4 5 9 10 11 (DUTCH) (AFRIKANER) D17S1787 4 4 4 4 4 4 4 8 4 8 4 8 4 1 D17S2231 4 4 4 4 4 4 4 11 4 11 4 11 4 6 D17S1793 4 4 4 4 4 4 4 7 4 7 4 7 4 4 BP7060 11 11 11 11 11 11 11 12 11 12 11 12 11 11 BP7872 2 2 2 2 2 2 2 3 2 3 2 3 2 2 D17S855 6 6 6 6 6 6 6 8 6 8 6 8 6 7 BP12568 1 1 1 1 1 1 1 3 1 3 1 3 1 ND BP12574 3 3 3 3 3 3 1 3 1 3 1 3 3 ND (D17S2250) BP12578 2 2 2 2 2 2 2 2 2 2 2 2 2 ND BP6991 3 3 3 3 3 3 3 3 3 3 3 3 3 3 D17S1789 Δ Δ Δ Δ Δ Δ Δ 8 D17S951 1 1 1 1 1 1 1 1 1 1 1 1 1 3 SOST C C C C C C C T JM6307 1 7 1 1 1 1 1 1 1 1 1 1 1 4 (D17S2253) D17S2234 2 1 2 2 2 2 2 2 2 2 2 2 2 2 D17S1860 7 11 7 7 7 7 7 7 7 7 7 7 7 10 BP7129 2 6 2 2 2 2 2 2 2 2 2 2 2 4 BP7141 10 1 10 1 10 1 10 10 10 10 10 10 10 8 D17S2235 3 1 3 1 3 1 3 3 3 3 3 3 3 3 D17S1861 8 13 8 2 8 2 8 8 8 8 8 8 8 8

Example 2

Sclerosteosis and Van Buchem's Haplotypes are Distinct

[0073]To determine whether the Dutch van Buchem disease haplotype was related directly to the disease haplotype found in Afrikaner individuals affected with sclerosteosis, sclerosteosis DNA samples were typed with the same set of markers described in Example 1. These were then directly compared to the van Buchem disease DNA samples. The analysis showed marked differences between the two disease haplotypes (Table 1). In instances where the two disease chromosomes carried identical alleles at adjacent markers, true identity by descent (IBD) was unlikely, due either to the fact that one or the other shared allele was found at a high frequency (>0.50) in normal unaffected controls (not shown), or to the close spacing (i.e., <50 kb) between the markers. Finally, the van Buchem disease samples were typed for a single nucleotide polymorphism (SNP) specific for the Afrikaner SOST gene mutation. It was found that they all carried the normal "C" allele. These results indicate that van Buchem disease haplotype is distinct from sclerosteosis haplotype.

Example 3

Van Buchem's Disease is Caused by a Specific Chromosomal Deletion that Represents a Unique Identifier for the Disease Chromosome

[0074]This example discloses that patients afflicted with VBD have a chromosomal deletion of approximately 52 kb that is correlative of the disease phenotype.

[0075]Characterization of all the candidate genes within the 1 Mb region between polymorphic markers BP12574 (D17S2250) and JM6307 (D17S2253) revealed a specific chromosomal aberration that segregated absolutely with Van Buchem's disease. Two independent experimental approaches to identifying the VBD candidate region revealed the presence of a chromosomal deletion associated with VBD.

[0076]First, a publicly available oligonucleotide primer pair (i.e. the SSLP designated D17S1789; see FIG. 1A and Table 5) failed to PCR amplify a DNA fragment from genomic DNA obtained from VBD patients while permitting the amplification of a DNA fragment from genomic DNA obtained from normal individuals. As part of the present invention, the nucleotide sequence of a 92,101 bp stretch of genomic DNA from a normal individual, encompassing the D17S1789 amplified fragment, was obtained and is presented herein as SEQ ID NO:1.

[0077]Second, the 92 kb stretch of DNA was computationally analyzed, using the GENSCAN exon prediction algorithm, to identify putative exon sequences (Burge et al., J. Mol. Biol. 268:78-94 (1997)). PCR primer pairs corresponding to sequences flanking each exon were designed and tested in amplification reactions using as template the genomic DNA obtained from non-VBD-afflicted individuals and individuals afflicted with VBD.

[0078]FIG. 1A discloses the normal exon structure across the relevant chromosomal region between the Mox1 and SOST genes, as predicted by the GENSCAN exon prediction algorithm. Exons that amplified from both VBD and normal DNAs are indicated by the filled blocks (i.e. 1-4 and 26-28) while exons that failed to amplify from VBD DNA are indicated by the open blocks (i.e. 9-19). The location of the amplicons that include these exon sequences within the 92 kb nucleic acid of SEQ ID NO:1 and the respective SEQ ID NO assigned to each of the exon sequences as provided by the present invention are indicated in Table 2. The results of these amplification reactions using genomic DNA from VBD afflicted and nonafflicted individuals is summarized in Table 4.

TABLE-US-00002 TABLE 2 Amplicons Spanning Potential Exons Predicted by GENSCAN Primers Location in Amplicon Name Amplicon # (DMO#) SEQ ID NO: 1 SEQ ID NO Gscn12.210A.09 19 12098/12104 14021-13436 SEQ ID NO: 17 Gscn12.210A.8 18 12097/12103 18353-17837 SEQ ID NO: 16 Gscn12.210A.7 17 12090/12096 18903-18334 SEQ ID NO: 15 Gscn12.210A.6 16 12089/12095 19556-18884 SEQ ID NO: 14 Gscn12.210A.5 15 12088/12094 19593-19063 SEQ ID NO: 13 Gscn12.210A.4 14 12087/12093 29498-28949 SEQ ID NO: 12 Gscn12.210A.3 13 12086/12092 33353-32666 SEQ ID NO: 11 Gscn12.210A.2 12 12085/12091 35036-34373 SEQ ID NO: 10 Gsch12.210A.1 11 12078/12084 36817-36212 SEQ ID NO: 9 Gscn12.668.08 10 12110/12116 49386-49957 SEQ ID NO: 8 Gscn12.668.07 9 12109/12115 50550-51151 SEQ ID NO: 7 Gscn12.668.06 4 9260/9261 58974-58394 SEQ ID NO: 6 Gscn12.668.05 3 12709/12710 58922-59740 SEQ ID NO: 5 Gscn12.668.04 2 12101/12107 67161-67717 SEQ ID NO: 4 Gscn12.668.03 1 12100/12106 75612-76103 SEQ ID NO: 3

[0079]In total, 16 fragments, corresponding to the SSLP (D17S1789) and at least 15 potential coding exons, were successfully amplified from genomic DNA obtained from non-VBD-afflicted individuals. In contrast, 12 of these 16 fragments did not amplify from genomic DNA obtained from individuals afflicted with VBD. These results revealed the presence of, and partially localized, a genomic deletion within human chromosome 17q21 in the locus associated with VBD (see FIG. 1A).

[0080]To better define the endpoints of this chromosomal deletion, additional PCR amplification reactions were performed with primers designed to amplify within the presumably non-coding regions located between the exons at either end of the VBD-specific deletion. The PCR primer pairs used and results obtained are summarized in Tables 3 and 4.

TABLE-US-00003 TABLE 3 Amplicons Corresponding to Non-coding Sequences Located within SEQ ID NO: 1 Primers Location in Amplicon Name Amplicon # (DMO#) SEQ ID NO: 1 SEQ ID NO 5'moxA 25 12697/12698 1499-1018 SEQ ID NO: 27 5'moxB 24 12699/12700 2524-2065 SEQ ID NO: 26 5'moxC 23 12701/12702 5608-4939 SEQ ID NO: 25 5'moxD 22 12703/12704 8684-8249 SEQ ID NO: 24 5'moxE 21 12705/12706 11248-10880 SEQ ID NO: 23 D17S1789 20 1789for/rev 14257-14060 SEQ ID NO: 22 668intronD 8 12622/12623 51371-52377 SEQ ID NO: 21 668intronC 7 12624/12625 53062-54232 SEQ ID NO: 20 668intronB 6 12626/12627 55163-56398 SEQ ID NO: 19 668intronA 5 12628/12629 57103-58202 SEQ ID NO: 18

[0081]The deletion associated with VBD was further characterized by amplifying a fragment from VBD genomic DNA, using the PCR primer pair 9260/12702 (SEQ ID NO:30/SEQ ID NO:77), sub-cloning this fragment and sequencing it to completion. As disclosed in FIG. 2, the 9260/12702 primer pair spans the deletion identified in VBD afflicted individuals. The 2317 bp fragment obtained from PCR amplification comprised a deletion breakpoint flanked on both the 5' and 3' ends with normal genomic DNA sequences. Without being limited to any particular theory of the present invention, it is believed that these results indicate that the VBD aberration is a simple chromosomal deletion, with no other associated rearrangements. The nucleotide sequence spanning the deletion breakpoint is depicted in FIG. 1B and is provided in SEQ ID NO:101.

[0082]Comparison of the SEQ ID NO:101 with the 92,149 bp sequence obtained from non-VBD-afflicted individuals revealed that approximately 52 kb of contiguous genomic DNA is invariably deleted from chromosome 17 at position 17q21 in individuals afflicted with Van Buchem's disease. This deletion is not present in individuals who are non-carriers or not afflicted with Van Buchem's disease. The sequence of the 52 kb sequence is provided in SEQ ID NO:2.

TABLE-US-00004 TABLE 4 Amplification Results Obtained from VBD Affected and Unaffected Genomic DNA Samples PCR Results Primers VBD Amplicon Name Amplicon # (DMO#) Affected Unaffected Mox1.03 28 12158/12164 + + Mox1.02 27 12157/12163 + + Mox1.01 26 12265/12156 + + 5'moxA 25 12697/12698 + + 5'moxB 24 12699/12700 + + 5'moxC 23 12701/12702 + + 5'moxD 22 12703/12704 - + 5'moxE 21 12705/12706 - + D17S1789 20 1789for/rev - + gscn12.210A.09 19 12098/12104 - + gscn12.210A.8 18 12097/12103 - + gscn12.210A.7 17 12090/12096 - + gscn12.210A.6 16 12089/12095 - + gscn12.210A.5 15 12088/12094 - + gscn12.210A.4 14 12087/12093 - + gscn12.210A.3 13 12086/12092 - + gscn12.210A.2 12 12085/12091 - + gscn12.210A.1 11 12078/12084 - + gscn12.668.08 10 12110/12116 - + gscn12.668.07 9 12109/12115 - + 668intronD 8 12622/12623 - + 668intronC 7 12624/12625 - + 668intronB 6 12626/12627 - + 668intronA 5 12628/12629 - + gscn12.668.06 4 9260/9261 + + gscn12.668.05 3 12709/12710 + + gscn12.668.04 2 12101/12107 + + gscn12.668.03 1 12100/12106 + +

TABLE-US-00005 TABLE 5 Polynucleotide Sequences Derived from SEQ ID NO:1 Primer Nucleotide Name Sequence (5'-3') SEQ ID NO 1789for ATTGGCCTGGCTTCTG SEQ ID NO: 28 (5522) 1789rev GGCTGGAGCAGGGACT SEQ ID NO: 29 (5524) 9260 TCCTGTGATCGCATTGAGAC SEQ ID NO: 30 9261 CCCTGCCATTCTGGATAGTTT SEQ ID NO: 31 12078 CAGTGGCTTTATTTTCCTAA SEQ ID NO: 32 12084 GAAGCTTCTCCATGTTCTTA SEQ ID NO: 33 12085 GTCTAAAAATGAAGAAGGCA SEQ ID NO: 34 12086 TTAAGTGACTTGTCCGAGAT SEQ ID NO: 35 12087 CAACTCAATCTTTTGGTGTT SEQ ID NO: 36 12088 AAATCAGATTCAAGCAGTGT SEQ ID NO: 37 12089 TCTTAACTGTCTTTTCAGAC SEQ ID NO: 38 12090 ATTGTTTCATTTTACCCTCA SEQ ID NO: 39 12091 GCCATAAAATCAGGATAATG SEQ ID NO: 40 12092 TGCCTAGAACATTCTGGTAT SEQ ID NO: 41 12093 CAAAGTGGCTCTGATTATTT SEQ ID NO: 42 12094 GGATCTCCTACGTACTGCTA SEQ ID NO: 43 12095 TGAGGGTAAAATGAAACAAT SEQ ID NO: 44 12096 AAAGACACTGCAGAGAAAAG SEQ ID NO: 45 12097 CTTTTCTCTGCAGTGTCTTT SEQ ID NO: 46 12098 GAGACCTCTCCTCTTTGAAT SEQ ID NO: 47 12100 GGTTTCAACTAGTTCTGGTG SEQ ID NO: 48 12101 CTAGGGCTTAGAAGTTTCCT SEQ ID NO: 49 12103 ACTCCTAGAACCCTAAAGGA SEQ ID NO: 50 12104 GTATCACCAGTGAAGTTGGT SEQ ID NO: 51 12106 GATAAATGGATATGGCAAAG SEQ ID NO: 52 12107 GGTTTATAATTTGCAACCAG SEQ ID NO: 53 12109 ATTCCCTAAGAGATTTGTCC SEQ ID NO: 54 12110 GAGAGGACAAACATTCAAAC SEQ ID NO: 55 12115 TTTAGACCTATCACTCCCAA SEQ ID NO: 56 12116 CTACTGGGACAAACCATTAC SEQ ID NO: 57 12156 AGAGAGGGTGAGTAACTTCC SEQ ID NO: 58 12157 AATAAAAGAAAGTTTGGGGT SEQ ID NO: 59 12158 GCAGAGTGCTTTTAGAACAT SEQ ID NO: 60 12163 AGGTGGAGGTTACAGTAAGA SEQ ID NO: 61 12164 AAGCAGTATCTCTGAAGCTG SEQ ID NO: 62 12265 CCTTTTCTTGGTTCAGATAA SEQ ID NO: 63 12622 ACGGTGTACACATTTGGTTAG SEQ ID NO: 64 12623 CGGATATTTGTCTGTGATACG SEQ ID NO: 65 12624 ACCGTGCAGAGGTAGATGGTA SEQ ID NO: 66 12625 CCAGTGGAAGAGACAGGTGA SEQ ID NO: 67 12626 GAGCTGAGATCGCACCACTT SEQ ID NO: 68 12627 CAACACGACATGGAATGGACT SEQ ID NO: 69 12628 CACAGCTGGAGACATGTTACA SEQ ID NO: 70 12629 AGGGTTCACACCATATCAGAA SEQ ID NO: 71 12697 ACGCTGCTGTTAAGGTCCA SEQ ID NO: 72 12698 TGCCAATTAGCCACACTCTTC SEQ ID NO: 73 12699 GTGCAAAGTGCCTTACACAG SEQ ID NO: 74 12700 GAGGTTAGACGGGTCTGAGTT SEQ ID NO: 75 12701 TGGCAGGCAGTAGTAACTCTG SEQ ID NO: 76 12702 CTGGGATTACAGGTGTCTGG SEQ ID NO: 77 12703 TGAGCTGTTCCCACACCACAT SEQ ID NO: 78 12704 TCAGGACGTTGCACTTTGACA SEQ ID NO: 79 12705 GAATGCTGGATGTGGATTGAG SEQ ID NO: 80 12706 GAGCAGAAGGCCTTGACTGA SEQ ID NO: 81 12709 GCCTTCAGTTTGTCCCGTTCT SEQ ID NO: 82 12710 CGCGAGGTCAGGAGTTCGAT SEQ ID NO: 83 12952 CCACTGAACAGGGCACAAGAT SEQ ID NO: 84 VBspan1/ GAATTACTGGCTGAGGCAACC SEQ ID NO: 85 12953 Span1F GCCTGCGCTTACATAGT SEQ ID NO: 86 Span1R AGGCGGGCGGATCACAAGGTC SEQ ID NO: 87 Span2F GGCTCACTGCAACCTCCACCTA SEQ ID NO: 88 Span2R AACCAAACAAGCCAGCAATCACTC SEQ ID NO: 89 Wt1R TGGGAGGCTGAGGCAAGAGAAT SEQ ID NO: 90 Wt2F ACTGGGCCTGGGATGTAT SEQ ID NO: 91 Wt2R AAAAATTGGCTGGGTGTGG SEQ ID NO: 92 Wt3F CAGGCTGGAGTGCAATGGTGTG SEQ ID NO: 93 Wt3R TGGGAGGCTGAGGCAAGAGAAT SEQ ID NO: 94 Del1F CTCACTGCAGCCTCAACTCG SEQ ID NO: 95 Del1R TAGCAGAAGGGGGAAGGGGGAACG SEQ ID NO: 96 Del2F GACGGTGTACACATTTGGTTAGTT SEQ ID NO: 97 Del2R GCACAGTTACATCCGCCTTATCGT SEQ ID NO: 98 Del3F TGCAGACACCCAGAGAAGACGACT SEQ ID NO: 99 Del3R TGGACCCTGCCCTCACGATGGA SEQ ID NO: 100 VBspan2 TACTACTGGGCCTGGGATGTA SEQ ID NO: 104 VBint1 TAGAGAAAGACCTCGTTATTGG SEQ ID NO: 105

Example 4

First Methodology for Identification of Van Buchem's Disease Carriers by Detection of a 52 kb Genomic Deletion

[0083]This example discloses a methodology for distinguishing individuals who are afflicted with or carriers of Van Buchem's disease as well as from individuals who are normal with respect to the 52 kb deletion. The methodology is based on the observation that the large, i.e. 52 kb genomic deletion that correlates absolutely with Van Buchem's disease may be identified by amplification of the region encompassing the deletion breakpoint.

[0084]The deletion on the Van Buchem's disease chromosomal locus 17q21 results in the apposition of DNA sequences normally separated by approximately 52 kb. A PCR assay specific for the deleted chromosome was developed. Starting with the sequence of the 2.3 kb VBD-specific fragment that includes the deletion endpoints, primers were designed to span the endpoints and yield a 400-800 bp amplicon from VBD DNA but would not, because of the presence of the 52 kb genomic fragment, permit the amplification of any fragment from normal, non-carrier genomic DNA samples.

[0085]As illustrated in FIG. 2, the following oligonucleotide primer pairs were used to amplify chromosomal DNA within the locus 17q21 from individuals who are afflicted with or carriers of VBD: Primer Nos. 12952 and 12953 hybridize within the region upstream and downstream, respectively, of the 52 kb genomic region deleted in VBD-afflicted individuals and amplify a 798 bp fragment when the 52 kb region is deleted; Primer Nos. Wt2F and Wt2R hybridize upstream of and within, respectively, the 52 kb genomic region deleted in VBD-afflicted individuals and amplify a 473 bp fragment in the absence of the deletion; Primer Nos. 9261 and 9260 hybridize within the region downstream of the 52 kb genomic region deleted in VBD-afflicted individuals and amplify a 581 bp fragment in all cases.

[0086]The VBD diagnostic was designed such that individuals who are homozygous for the 52 kb deletion and therefore afflicted with VBD may be differentiated from individuals who are heterozygous for the 52 kb deletion and therefore carriers of VBD as well as individuals who are normal with respect to the 52 kb deletion. Two separate PCR reactions were performed on the individual to be tested. In the first, a primer pair spanning upstream and within the VBD deletion (i.e. the Wt2F/Wt2R oligonucleotide primer pair) yielded an amplicon from normal non-carriers and heterozygous carriers. In the second reaction, primers which span the 52 kb deletion (i.e. the 12952/12953 oligonucleotide primer pair) yielded an amplicon from heterozygous VBD carriers and homozygous patients. A primer pair spanning a region unaffected by the 52 kb genomic deletion (i.e. the 9260/9261 oligonucleotide primer pair) was included as a positive control for the PCR reactions.

[0087]The following reaction conditions were used for amplification of 400 to 800 bp nucleic acid fragments using as template genomic DNA isolated from patients who are afflicted with or carriers of Van Buchem's disease: 25 ul reactions included 10 ng genomic DNA; 60 mM TrisHCl, pH 7.5; 15 mM Ammonium Sulfate; 2.5 mM Magnesium Chloride; 0.4 mM each of dATP, dCTP, dGTP and dTTP; 0.2 μM each of Primer Nos. Wt2F and Wt2R, or 12952 and 12953, or 9260 and 9261. The fragments (473, 798, and 581 bp, respectively) were amplified by the following protocol: Denaturation at 94° C. for 3; 40 cycles of 94° C. for 1 minute, 68° C. for 1 minute and 72° C. for 1 minute; final extension at 72° C. for 5 minutes. The amplified nucleic acid fragments were resolved on a 1% agarose gel using 1×TAE buffer. DNA bands were visualized with ethidium bromide staining.

[0088]The results are summarized in FIG. 2. The summary shows that nucleic acid fragments amplified in all PCR reactions containing the 9260/9261 oligonucleotide primer pair regardless of the source of genomic DNA template. The 12952/001 oligonucleotide primer pair amplified a nucleic acid fragment from non-VBD afflicted individuals and individuals who are heterozygous for and therefore carriers of VBD but did not amplify a nucleic acid fragment from individuals who are homozygous for and therefore afflicted with VBD. The 12952/12953 oligonucleotide primer pair amplified a nucleic acid fragment from individuals who are heterozygous for and therefore carriers of VBD and from individuals who are homozygous for and therefore afflicted with VBD but did not amplify a nucleic acid fragment from non-VBD afflicted individuals.

[0089]These results demonstrated that a first methodology presented herein permit the differentiation between an individual who is homozygous for the 52 kb genomic deletion and therefore afflicted with VBD, an individual who is heterozygous for the 52 kb genomic deletion and therefore a carrier of VBD and an individual who does not bear the 52 kb genomic deletion and is therefore normal with respect to VBD.

Example 5

Second Methodology for Identification of Van Buchem's Disease Carriers by Detection of a 52 kb Genomic Deletion

[0090]This example discloses a second methodology for identifying individuals who are afflicted with or carriers of Van Buchem's disease as well as from individuals who are normal with respect to the 52 kb deletion. The methodology is based on the observation that the large, i.e. 52 kb genomic deletion that correlates absolutely with Van Buchem's disease may be identified by amplification of the region encompassing the deletion breakpoint.

[0091]As illustrated in FIG. 3, the following oligonucleotide primer pairs were used to amplify chromosomal DNA within the locus 17q21 from individuals who are afflicted with or carriers of VBD: Primers VBspan2 and VBspan1 hybridize upstream and downstream, respectively, to a region that flanks the 52 kb genomic region deleted in VBD-afflicted individuals and amplify a 642 bp fragment when the 52 kb region is deleted. Primer VBint hybridizes within the 52 kb genomic region which is deleted in VBD-afflicted individuals and with primer VBspan2 amplify a 720 bp fragment in the absence of the deletion.

[0092]The VBD diagnostic was designed such that individuals who are homozygous for the 52 kb deletion and therefore afflicted with VBD may be differentiated from individuals who are heterozygous for the 52 kb deletion and therefore carriers of VBD as well as individuals who are normal with respect to the 52 kb deletion.

[0093]A single multiplexed PCR reaction was performed on the individual to be tested. The pair of oligonucleotide primers span the 52 kb deletion (i.e. the VBspan1/VBspan2 oligonucleotide primer pair) yielded an amplicon from heterozygous VBD carriers and homozygous patients. The second pair of oligonucleotide primers pair spanning upstream and within the VBD deletion (i.e. the VBspan2/VBint1 oligonucleotide primer pair) yielded an amplicon from normal non-carriers and heterozygous carriers. A primer pair spanning a region unaffected by the 52 kb genomic deletion (i.e. the 9260/9261 oligonucleotide primer pair) was included as a positive control for the PCR reactions.

[0094]The following reaction conditions were used for amplification of 400 to 800 bp nucleic acid fragments using as template chromosomal DNA isolated from patients who are afflicted with or carriers of Van Buchem's disease: 50 μl reactions include 20 ng genomic DNA, 0.4 μM each of Vbspan1 and Vbint1, 0.8 μM of VBspan2, 0.2 mM of each dNTP, 120 mM TrisHCl, pH 7.5, 30 mM ammonium sulfate, 5.0 mM magnesium chloride and 2.5 U Taq polymerase (Roche Molecular Biochemicals, Indianapolis, Ind.). The fragments (642 and 720 bp) were amplified by the following protocol: Denaturation at 94° C. for 3 min, 40 cycles of 94° C. for 30 sec, 63° C. for 30 sec, 72° C. for 2 min and a final extension of 72° C. for 5 min. The amplified nucleic acid fragments were resolved on a 2.5% agarose gel using 1×TAE buffer. DNA bands were visualized with ethidium bromide staining.

[0095]The results are summarized in FIG. 3. The summary shows that nucleic acid fragments amplified in all PCR reactions containing the 9260/9261 oligonucleotide primer pair regardless of the source of genomic DNA template. The VBspan2/VBint1 oligonucleotide primer pair amplified a nucleic acid fragment from non-VBD afflicted individuals and individuals who are heterozygous for and therefore carriers of VBD but did not amplify a nucleic acid fragment from individuals who are homozygous for and therefore afflicted with VBD. The VBspan2/VBspan1 oligonucleotide primer pair amplified a nucleic acid fragment from individuals who are heterozygous for and therefore carriers of VBD and from individuals who are homozygous for and therefore afflicted with VBD but did not amplify a nucleic acid fragment from non-VBD afflicted individuals.

[0096]These results demonstrated that a second methodology presented herein permit the differentiation between an individual who is homozygous for the 52 kb genomic deletion and therefore afflicted with VBD, an individual who is heterozygous for the 52 kb genomic deletion and therefore a carrier of VBD and an individual who does not bear the 52 kb genomic deletion and is therefore normal with respect to VBD in a single multiplexed amplification reaction.

[0097]In providing the forgoing description of the invention, citation has been made to several references that will aid in the understanding or practice thereof. All such references are incorporated by reference herein.

[0098]From the forgoing, it will be appreciated that, although specific embodiments of the invention have been described herein for purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention. Accordingly, the invention is not limited except as by the appended claims.

Sequence CWU 1

105192139DNAHomo sapiensmisc_feature7043, 8369, 8401n = A,T,C or G 1caaagatttg attctttata ctttttatgt tcaaattttt aaaaatgcaa aagaaaaaaa 60actaaatagg agggaaaaat gttcaacaga aaatttaccc caggaaccaa aaaaaaaaaa 120aaacccaaaa ctaaagtctc tccttaaagt acacacacat gtggccagct actcctatgt 180gtgtgcacac acctatgtcg ggcttgctcc tgcccctcca atgcaccagc ctacctactg 240ggatcccctg tatgtgtatt tgtcgccaat gacactaaac attttcactg tcccaaccca 300gacgcaccag ctgacgagga gttcgtcctg gagcccagag caaatgcctg cagagggctg 360gaccagggct gctgggacac acttttaaat cctgcggtgg ggagagagtg acaaatttct 420gaagtgaaat gtgagagagg agagggaggg gttaacccac acacacaaga tcccctccaa 480ccaaaaccct agcaggcaac tattaatcat cagaaacctt atatgcacat ctaatgggat 540ttgcagatgg agacttttaa agaaacattg tctgtatttt ttttttaagg aaagagagca 600gcacacacac acatacacac acaccgtctt taatgtgcct cctgtaacac tatgaagtta 660tttttattgc actgttaaca aaattcccca agtcttggat ttagaaaaag ccttaagtca 720gatccagaat ccatcaaatg ccaaattcca gggagtgaag tgaggaataa gagcaagaga 780caagcttggt gattgcattt gatttaaagg cccttctact gctgcctgcg aaaaaggaag 840gtggattaaa agaaatcttt tttgcaagtc tcagcggccc actgtggtct gcagtacaaa 900gaaaggcaca ctctagaaaa aaagaatttc tctctgccca gagtgacatt ctcactttct 960cagtaactta aaaaaacaat cagattcttc cttccgctct ttaacctcca acatacatgc 1020caattagcca cactcttctc tagagaggtt tcaagtcatt tttcttcaca gcaatggcaa 1080ggctcttaaa taaggtcctt gaagtttctt cgggtctcct cccacctgct ccctgccccc 1140ttcacctcca cccacctgct tccctttctc atcccccaga ggcggaggct tccgaggaat 1200ttggggtagg gaaataggaa tcaggggctc ctcattcccc aaaggagcct ctttggcaag 1260caggcacgtg ggtctccggg ctggtgcaca tcacagggca gccagcccaa gtgccatctt 1320gatgcccaat cagtcttcct catggctgcg ccctgctggt ctctcagagg gttaatgcaa 1380tttcttggag gacgacattc ctaacaccca ggggccagaa ctccttcccc actggttatt 1440cccatggccc agagcagcag gatgggggca gaaacaggca tggaccttaa cagcagcgtc 1500tctgctggct gcttccaaag acggcgaggg ttccctgagg aggggcccct aggtcttagc 1560tctgcagcct ggaggtgggg ccctgggggg tgggtgcagg agatgctcct aggaggagcc 1620gaagggcacc cagggatgtg gaatgaagcc acgactggcc tttaatccag aaactccagt 1680cagatacaca tcctgggagc aggagcagct gtaaaagatg gggaggggtg tacatatgaa 1740aagatgtgcc ctcttctgga acaagcggtc agagtgttgt agaccagtag agtggatggg 1800gtcatagctg tcaaccagac tcatctttct gctttgccca gggcttagct gtggcacaca 1860caattctctg agcctcacaa ctttcctagg atgggtaagt aagaattatg tctccatttt 1920gcagatgaca aaaccgaggc tcagatggat gactgggctg cccagactca cacagctaag 1980aaatggtgga atcagcactt ggcctcaggt gtcctgctgt tcagtttgga gcaggccaga 2040tgcagaggag gaggcatggg gtttgaggtt agacgggtct gagttcaagc cccaggtctg 2100ccacttcctg gctgtgtgcc ctggaacaag tcaccttatc tctctgaact tctttttttt 2160ttttttcttt ttgagatgga gtctcgttct gtcgcccagg ctggagtgca gtggcatgat 2220ctcagctcac tgcaagctcc gcctcccggg ctcacgccat tctcttgcct cagcctcccg 2280agtagctggg actacaagca cccgccacca cgcccggcta attttttgta ttttttagta 2340gagacggggt ttcaccgtgt tagccaggat ggtctcaatc tcctgacctc gtgatccgcc 2400cgcctcggcc tcccaaagtg ctgggattac aggcatgagc caccatgcct ggccatctct 2460ctgaacttct gtttcctcat ctgagatgac agtcagagtg ggatctgtgt aaggcacttt 2520gcaccttaca catttttacc cccacggatt cccacaaaag ccttctgagg caacagtcat 2580tctcatcagc tccgtttctc tctctctctt ttctttttat cagctccgtt tttcagcaga 2640agactctgat gcacagatag actgtgtaac ctgcacaagg ccacacagca gggaagcagc 2700ggacccagga ttcaaattga ggcagcccag cccacagaca tttgcaaatc ttcaatgaaa 2760tcctttatgt aggccaggca cggtggctca gacctataat cccagcactt tggaaggctg 2820aggcaggcag atcatttgag gccaggagtt caagaccagc ccggccaaca tggtaaaacc 2880ccgtctctaa tagaaataca aaaattaggc cgggcgcggt ggctcatgcc tgtaatccca 2940ccactttggg aggccgaggc gggtggatca tgcggtcagg agatcgagac catcctggct 3000aacacagtga aaccccgtct ctactagaaa tacaaaaaaa aaaaaaaaaa ggcgggcgtg 3060gtggcgggcg cctgtagtcc cagctacttg ggaggctgaa gcaggagaat ggtgtgaacc 3120cgggaagcag agcttgcagt gcaccgagat cacgtcactg cactccagcc tgggtgacag 3180agcgagactc catctcaaaa aaaaaaaaaa aaagaaatac aaaaattagc tgggtgtggt 3240ggcacatgcc tgtaatccca gctactcggg aggctgaggg caggagaatc gcttgagcct 3300gggaagcaga gaggttgcgg taagccaaga ttgtacaact gcactccggc ctgggcgaca 3360gagtgagaca ctgtctcaaa aaaaaaaaaa aagaaaaaga aaaagaaata aagaaagaaa 3420tcctttatgc aaaaggcaga gtaagtaatg gacaaatgtg gctcccttcc tagcacatct 3480atttctggtt aacctcgatg atcccaaagg gtgaacctgg gaatggggag ttctgaggaa 3540attctacaga aacagccttg tgaggtcctt tgtggggggc acgctgtgct gtgggggttc 3600tggaaggaat ccgtgggagg ctgggaggaa gatctggctt gtcagcttcc ctaggaaaac 3660cttcccctgg gctggccgca ggctgtaacc ggattcctgc tccacctctg catctggccc 3720agggacctca tggcagggag gcccagcgcc tggccctttg cccctggacg gggtgggccc 3780tgggtcatgg tggggtgggt ggggaggtca ggagggccat ggggaggggg cgcggtgggg 3840tgctttgccc tgagaacaca ggcctctggc accccggagc ccccggcagc tgctggcgtc 3900tgtcagccac cttgcggggc gcggccgggg gcctgctggc ccctacatct tcctgacagg 3960cccctcttct gaggccagga aaaaacaaca acagttcctc ccctcacggc aacccatttg 4020ttagatgaag gccgggcacc agcaccttta acctcctcaa agtcagcgtt tccctgtcaa 4080ggccccacag ggccagagac agagatggat ggaaggagct gtgtgtcgaa aaagccctgt 4140ggcctcatga ggagagctct gttttcagga agggagggga ccccggtttc tgattgttgg 4200ggagaataag gggaggaaga ggaaaagtgt gagtcacgag gaggtccccc aggggcgtgg 4260gggggcccag gaggctttca gcctggccac acctgagcca tcacgtggaa cttgcggaat 4320gtctcccatt gtgcgtggca ggcaggcgtg tacttggcag gacagggctg cttctgtttg 4380tggccacccc acccccttgt gcttggagag gcagggtgtc agggcaaggc cctggacctg 4440gaggcagaag acacgggttc aaggtgtgac cttgcctgtt accggctgtg tggtctcagg 4500caaatcacac ccttctctga gcttcatttt tctcactggc aaaatggagt tgacagttcc 4560ggcctcacct ggcggttagg aggataaatg aactgcatct gagaacagag ctggctgact 4620ataaagggtg atgcatgtga ggaatagctt gtccttaact atgctgaacc actgggcagg 4680acacagcaca atgtaggtga agacggctca tcccagcccc cagatacctt ctatggcaac 4740ataacacaag ggtccaaatc atggccttgg gaattggggg cctgtgggtt caaacctcag 4800ctctgttttt ttgttttttg ttttgagacg gagtcttagc tctgttgccc aggctggagt 4860gcagtggtgg gatctcggct caccgcaacc tctgcctccc aggttcaagc gattctcctg 4920cctcagcctc ccgagtagct gggattacag gtgtctggca ccatacctag ctaatttttg 4980tatttttagt agagatgggg tcttgccatg ttggccagac tggtttccaa ctccccacct 5040caggtgatct gcccgccctg gcctcccaaa gtgctgggat tacgggcatg agccactgtg 5100cctgacctca gctctgttat taataagcta aatggctttg agcgacttgc cttatcactt 5160gagcctcagt ttcctcatct gtaaaatggg gataaacttc ttccgtccgc atgaggatgc 5220tgagagacgt gagtgaggtg gtctatgaaa gctcttgtca tagcctggca tgcaggggta 5280acatctggat gatgaagatg atgatacctg agatttttgc cttacagaca actccagaga 5340gccctgtgaa atatttatat gccactgaac agggcacaag atgaagccat tagcctgcgc 5400ttacatagta gaatgtgtga atcagatgag atgcttggtc tctagtaaga ccttaaggga 5460tggacagaag acaggcagat tttggatatg gtatacgtgg ctgtgggcta gcgtgtttac 5520tactgggcct gggatgtatt tggaatgtac acatgtgtcc tttgcttctc agaacacttt 5580gaggcagcag agttactact gcctgccagc ctgcctcaca gggttttttg ttttgttttg 5640ttttcttttt ttttgagatg agtttcactc ttgttgccca ggctggagtg caatggtgtg 5700atcttggctc actgcaacct ccacctactg ggttcaagcg attctcctgc ctcagccacc 5760tgagtagctg ggattacagg tgcatgctac catgcccggc taatattttt tatttttatt 5820tttatttatt tatttatttt tgagatggag tctcgctctg tcaccaggct ggagtgcagt 5880ggtgcaatct cggctcactg caacctctgc ctcccaagtt taagtgattc tcttgcctca 5940gcctcccaag tagctgggac tataggcacg cgccaccaca cccagccaat tttttgtact 6000tttaatagag acgaggtttc actatattgg ctagggtggt cttgaactcc tgacctcagg 6060tgatctgccc gcctcagcct ccaaaagtgc tggaattaca ggcatgagcc accgcgcctg 6120gccctgacag ggctttgtaa tgctcaaata atatgatcca agggtcagag ttgagtaaac 6180tatagaacat agtccttgca ttccacccca ggggcccaat aacgaggtct ttctctaaat 6240ctcagaaaac actaagctaa gggaggtaag ttctgcagat gggttttctc tgtgtgtgtg 6300tgtgtgtgtg tgtatacata tatatataca cacacacata tatatatata cacacatata 6360tatacattta aacctttggt gctctacttg tctgctacca aaattgtagg cagatgactg 6420aaaaatgtaa cacacagaat ggcctgcata gaaacctggc tcttggaagt catatgagtc 6480agtttcctca actgtaaagt ggtggctcac tgtacagaca ctcagtaagc acccagcaaa 6540tgaacacctt ccaggggttc tgtattggag tcagctgtat agaatgaaaa caaatgacaa 6600aaggggaaac acacagagtt tattattctc ccccataaaa aaggccggag gtgacagtcc 6660aagctgagat ggcctctccc ccaggcactg tctctctact ttgcccccct gcggcacctt 6720gttcagctgg catgtcagca ttccacacag cacgaaggag gaaggacaaa tggactctcc 6780ccatctctct gcccactgcc cccctcccca caacccgttt aaaaaataga tatggggggc 6840cgggtgcggt ggctcgtgcc tgtaacccca gcactttggg aggccgaggc gggtggatca 6900cgaggtcagg agatcgagac catcctggct aacacggtga aaccccgtct ccactaaaaa 6960tacaaaaaat tctccaggca tggtggtggc aggcgcctgt agtctcagct actctggagg 7020ctgaggcagg agaatagcgt ganccgggag gcgtagcttg cagcaaggcg agattgcgcc 7080actgcactcc aagcctaggc gacagagcga gactccatct caaaaaaaaa aaaaaaaaaa 7140aaatagacat gggggcctgg cgcggtggct cacgcccgta atcctagcac tttgggagtc 7200cgaggtgggc ggatcacgag gtcaggggtt cgagaccagc ctggtcagcg tggtgaaaat 7260ccatctctac taaaaatata aaaatcagct gggcgtggtg gcgcatggct gtagtcccaa 7320ccactcagga ggctgaggca ggagaatctc ttgaacccgg gaggcggagc ttgcagcgag 7380ccgagatcgt gccaccacac tccagcctgg gcgacagagg cgagactccg tctcaaaaaa 7440aaaaaaaaaa tagacatagg gtcttgctat gttgcccagg ctggtcttaa actccttcct 7500tgagtgattc tcccaccttg gcttcccaaa gtgctgggat ttacaggagt gagacacagt 7560gcccagcctc tctacatttt tttttttttt ttttaagaca gactctcact cagtccctca 7620ggctggagtg cagtggtgtg atctcagctc actgcaatct ccacctccca ggttcaattg 7680attctcctgc ctcagcctcc caagtagctg ggactacacg cctggctaat ttttgtattt 7740ttagtagaga cagggttttg tcatgttggc caggctgatc tcaaactcct gacctcaaat 7800catccacccg ccttggcctc ccaaagtcct gggattacag gcatgagcca ttgtgcccaa 7860ccctccctgc cctgttttaa ggagggtcct gttcaacaat tactctacag accaattgcc 7920cgcaactata tacttggcag tgaggaactg ctggggaggc tgggagattc agcctatagg 7980caccttggtg gccctaataa ctcttttatc ttcttcttct tccccatcag aacctgttct 8040caaggaaaag agctaaggta ggctgccaga taaaatgctg aggtaagtct gttccagaaa 8100atattgggac atagactaaa aaattgttgt ttgtttatct gaaactcaaa tttaactgag 8160tgtcttcttt tccccctaaa tctggcaatc ctaatccaag gctaaactca ttttcaggac 8220gcaaaaggct ctggccttac cttttgagtc aggacgttgc actttgacag aaggctctgg 8280aaggaaactt taaagggagc cttccagagg gaaatgcggt gttggggtag gtctgccttt 8340ggctatgggc tttctggctg ccggagggnc ccagggtccc ccaggaaagc cttctgtgga 8400nggtcttttg agagagacaa agcagagggg tggaggaagg gcggctcagg tggaaggagt 8460gaggacaaag gtgagtgccc ctgggcagga agtgctgaaa gagagaagga gggaggccac 8520caggcctggg cctggagcca gcctgggaga ctcccagccg cccacttctc ggggcctccc 8580ttttccagcc ccttgctttc gaggcagcag tgccattatt tggggaaacc agctaaccag 8640ataggacagc aaaccgggga tttatgtggt gtgggaacag ctcaggtttc cctccctgtt 8700tacccagcag tattttttaa aacagaaatc agcgtgtggg taaccgcagc tgtgagttac 8760tagctctggc tgtgagggct ggggtggggg gagtctcttc agagccctct gtccactggc 8820ctgggagcta ctgaaggaat gtgcctctcc ccatcccagg ccaggtggag aaggtggccc 8880tgcggaagtt cccagatcac tgcccacctc acccttcccc tcccgacgaa ggccagcaca 8940cctgggggag gtgtgatgat ggttcaaggt ctaaagcttt agagatcagt cagtttaggg 9000gtcagaaccc atggagccag gcaagtaaac acaggttccc caagccagct gggagggaga 9060cacctgggtg cctttgatgg gagaaagagg gggccaacag ctacttggca ctggccaatt 9120ttcccttgca tgaacatggg cccagtgtaa ccaaccatat ctttccattt gtcaaaaaaa 9180agccatattt ccagattttt tataggcaac ctgtcaactt ttaaatgttg gcaatgaatt 9240caaaacactg ccattcaatt aaaagcagcc cagaacatgg cacgactggt tcttggaata 9300ccaatctgca atctctgatc ttgtccaact ctctctgctc cccattttac agatcaggaa 9360gctgaggccc agagaggcct agggacttag ccatggctgc agcagttgtt attgggaatg 9420ccatgaggcc aggatgcctg ttatgtgttc tttgcccagc ctagcagttt ggctggcttg 9480gctatgccag aggtctcacc atgcagttct caagtgcttc ctgagcatct ctcatttgca 9540tagacatgac atttacaaac acatccatca tctacttgat tctggcaatg acaccgaggg 9600agcagaactg gcattaatcc atttttttta atggtgagaa cactgaggtt tggagaggct 9660ggtgacctgc caaagtcaca gtcctagaaa gggctgcagc cagggcctcc aattacagat 9720caagggtttt tccaccccca aactcagtca tctcgtaaaa acgtcaggct ccttggggaa 9780tacagcttga gatggataat caactcctcc taaaagggaa ccataaatgg atgagatact 9840tgggtaaact gaatattctt tgtaacttaa agttaattga aaatccatag gttatcaatt 9900ttccaagcag gagaataaaa tagaaggtat ttcaaagagc aattatagta ggcatggctt 9960aatctttctt tgatgattct gaaggcattt caggggaatg taatgcctta gggcatcatt 10020atgaacacca ataattactg tgtagggctg tgacgcaaat tctcatcaat cccctcctgg 10080ctgtcggggt gttttcagag aggatcatgc agggagtgtg aacgtgtgcc tgtctgtttc 10140ttgctgctct ctccgccttt gtcaatttca gggtgctgtt gtggaaaatg cagtgtccct 10200ggaaggggga ggtcacttaa ccttaatgag cttctgtttc tttattaaaa tggggtaatg 10260cagactccta tactcactgt caaggttttg tggggctcag atgtcagtgt cagagcagga 10320agaaacttta gatcatgata tgcaccagct tcattctgcc atggaaaaat ctgaggccca 10380gagaggttag gagcctcgtc caaggtcacc cagccaggga gagggtgcta gaacctgggt 10440tttctgcttc ctaacctctt tcctgaatat aacactatgg aagaaaaaga tctggaggaa 10500cggaaagatg aacacatgat tacacatgca aacaatactg gggtctctct gatcagagaa 10560ggtgtgatct tccagcctca ggagaggggc catccaagtc ctggaggagg tgaacccctt 10620agttcagggc tgggagtagc ctgggggcag cagagacact gctgtgaggt ttatagtttc 10680atgactgtca gagcttttta aaatgtggta attttaagtg tgcagcctcc cagggtcttt 10740cttcttttaa ttgaagaaat aaaccatctc ccctaaggca tgcttggcga aggagaaagg 10800caggtgcaag gctcacagag gagagcagca gcctagaagg gctctgtgtc atggggaagt 10860aaaacatccc agaaacagag agcagaaggc cttgactgag ccccaggaga ggcaggacac 10920caggggtgca cacccataaa cacacacata cacatgtatg tctcctccct ggagcctgag 10980agtccctata tacagcaggt gcatgtgggc cacacatcac acaaaattga atacaggcag 11040gctcagagca ccagcacaca cgtatgtcct tgacaccctt agagatacta ctaagcacgt 11100gtgtgtacct gctcacccat atggcagagc ccctggatct gggcagaaat gccaaagcag 11160gggcaggcgc gtgtgcgcgc acacacacac acacacacac acacacacac tagcacagcc 11220acaaaagctc aatccacatc cagcattcct aacaacacac acacagctag acacgctagg 11280agacacatca ggacaatgtt tccactcccg ctgccataca catatgcagg tccacattca 11340ccagtggggt agggtagagt ctcatcagtc cagactcgca gacagctgga cacagaggtg 11400atctctgaaa cccaatgtct acacactgtg gtctttgtta cacacacaca cacacacatt 11460gaaatgatgt cctcagcctt tggttatttt tggtttcttc tgagctggag tttcactctt 11520gttgcctagg ctagagtgca gtggcgtgat ctcagctcac tgcaacctcc acctcccggg 11580ttcaagtgat tctcctgcct cagcctccca agtggttggg actacaggcg cccgccacca 11640caccctacta atttttgtat ttttagtgga aacagggttt caccatgttg gccaggctgg 11700ccttgaactc ctgacctcag atgatccttc cgccttggcc tcccaaagtg ctgggattac 11760aagcttgagc caccgcaccc ggccgagcct tcgggtattt tgaaagctga atgtgtggtt 11820acattttctt ttctttttct tctttttttt ttgagacgga gtctctctct gtcaccaggc 11880tggagtgcag tggcgcgatc ctggctcact gcaacctctg actccccggt tcaagtgatt 11940ctcctgtctc agcctcctga gtagctagga ttacaggcat gcaccaccac gtccagctaa 12000tttttgtatt tttagtagag ccagggtttc accatgttgg ccaggatggt ctccatctcc 12060tcaaatcgtc atccgcccgc cttggcctcc cgaagtgctg ggattacagg cgtgagccac 12120agtgcccggc ctacgttttc aaacagcaat agcattcgcc tcctctgtca gtttaacccc 12180catcacaact cccacttttg gcacctaaac agttaatttc ccagttcatg ggccttcaaa 12240gtcctgctct agtctctgga ggaactttca cctacagagg aaggtgtaag ggaaactagt 12300tcatggattt aagtagaaac attttaggtg tagctttcac atacagagga gtgagaaaaa 12360actgattcat ggatttagat ggaaacattg tagtatgaac ccagcggagg gtctgggagc 12420gccttctggt ggtgagaatt agaaccgcag cactttctgc aatgtgccca ggccagaaag 12480ctctaccttc tgataggacc cacttctgac cctagaatgg gggaactgat ggaggtgtca 12540agccactgtg gtcccacagc tgcatgcagg cacaggggat aggaagagag ctacctacag 12600gttactaaac cattcccttt taaaacagca ccaggcttat gtctactctg cgcttccatt 12660ttctaggttt taagtggaag atatgtgaac acccagtggg ctggatggct gtccctgcta 12720caagtctgtg atgtctccgt ccagtggcag agctggaagg caggtgctgt cggggctgca 12780tctgccttgt tcaccagcat aggcctaaaa ccatggaggg ggtgctttgg cttagatccc 12840cacttggcct gtgtgtgtaa gaggctctca ggcaccttaa tgctacatca ccaaccaaac 12900ctcctgatga ttcttttagg ttctccgttt ccaggcagat tcacttctgt agatttattt 12960atttattttt gagacagggt ctggctctgt cccgcaggct ggagtacagt gatgcaatct 13020cggctcactg tagccttgac ctcaacttca agtgatcctc caacctcagc ctcccagtat 13080gagggaccac aggtgtgcac catcacacct ggctaatttt tgtacttttt gtagagacgg 13140ggtctcacca tgttgcccag ctggtctcaa actcctaggc tcaagcgatc caccaacctt 13200ggcctctgaa atgctgggat tccaggtgtg aagcaccgcg cccagtccct gacttctgta 13260gacgtttgta ttgtttacat ctactgtgtg caatgtacga gatgcagtca ggtgtctgga 13320tggaccaggg gatctggcat cttatagact tgggtttgaa ccccaactga gccatttact 13380ggctgggtga ctttgggtga gttcttaaac ctctctgacc cttaaggtgg taacagtatc 13440accagtgaag ttggtgcaca cagcagggcc caaactgaca tctcacagca cccagcaggt 13500cactgtgggc atgataggat gatgggtcac tgtgccagcc ctgaaggagt tcaagtccag 13560ataggggaag gtggtggacc agacccagac agagattctg agtcgctgct gagactgggt 13620gagggtagtg ggtacatggg aggacatata gcccggcagc ccagggctgg agtccacact 13680caggttgggg cagcctggtc tgcctctcct gcaggagact tttccaggca ggcttgtccc 13740tccagaatgc acgaatcaaa tcctctcagg atcagtctca ttttcctcgt gctgggggag 13800caggctactc acagaagatg ttgttgcaaa tgtaagaatc acatgtcgat ccacaaactg 13860gcattgagca gctacctagg agatcaaaga aactcttact ttgggagctc ctgccagggc 13920tctttgggag gtctggctag ctctggagga agagaatgaa cttggggagg gcgtggaaca 13980gatgaggacg caggcactgc cattcaaaga ggagaggtct cccggacagg gctggcctgg 14040gcaggcccag ggagggtggg gctggagcag ggacttgaaa aagggagagg gctcaggaga 14100ctcagaggag gaggaaagtg tgtgagcagt aggcagggtg tgtgtgtgtg tgtgtgtgtg 14160tgtgtgtgtg tgtgcacgcg cgcgcatgca ggcctgtgta gggctgggaa agaacaaagc 14220aaaagggtgc acaaggcatc cagaagccag ggcaatgcag aacaggaaca aagcagtttg 14280gctcaagggg aaagttctga tggaaagcaa ggaaagagaa tgttagaagg gctgagagcc 14340agaatgccca gtatggggta cagagggcaa cagaggggct gatgttggtg gcgaggtggg 14400gaggagcatt tcacagggaa ctgggcagct ccaaggatgg gctggaggag ggacggtcct 14460aaagggggtg gtgggagaca ggcagttagt gatgggggga gagaaatgct ctgagggggg 14520ctgctgtagg cagggagggg caggtagcag gaggcagagg gttgagcctg cagaagcccg 14580gagcagtctg tagctttcta atgcttccta gggaattctg ctgctgagta gccatgggtt 14640caacaggtct ctaggatttg ccttgaatta ctgccctgag gccccctaag tctggggtct 14700cataaattca gcggggaggg cacacctgtg tctgagtctg agcagcccag agcccccagt 14760gttgtcagtg aacagctcac tgaacttgca gagcaattaa atcccagggc agaagaaaga 14820gcaggagggc agggtggccc ccagctccct cccatcccgg gtttggggag agacacctct 14880ggcctgagac tcctcgtggc ctctaacaag tcttgctctc tctctctttt tttttttttt 14940ttaaattatt gtctcccgcc ctccttcctt cttcttggga atcagaaaag aaacctcaat

15000gcctggcttt gcccctccct ggctgtggtc aagccatgtg acatttagac atttcagagc 15060ctcctgactc ctgaggctgg agacagcatg aagccaaatg ggcttggcaa acagaagaga 15120ggggcagtta acattctctg caatgctctc ttacttcccg tttaaacacc cctcctactc 15180ggacacagac ccccaacccc tgtgccattg tgctcactcc caccttggag gggtcaggct 15240gtggtgaaag gtcaaggtga aaagtcatga tgatcctatt gctgccacct ccctggggtg 15300agcaagattc tgtggtggct ccagctctcc catggcagtc cacacagagg ctgttccagg 15360ttctggagag gtcacttggt gtgacctggg aatctgcatc ttttctctgc caaagcagag 15420ggcattgcag caaccacacc tgggtcccag tcctcaacaa agtccctcca gctcccgctg 15480ctgtctgcaa ggtttggtca tctgtccctg cattggagaa gagtttgcac tgtgaatctt 15540gcctccacca gggaaggctg gtaaaagtcc ctgccccggg ggccctcatt gctccagggt 15600gcatctgggg gccagacctg tggaccctca cccttgagcc tcattttgtt cctcttagaa 15660caaagccatt tctggtaaat aggtttgatg ggtttggcag cagggaaggc acagaacctt 15720tcacgattag caggctcatg acaatttctc tttaggcaag agagaaggtt gaggaggaaa 15780gactggcagg gcttggagag ctggaaagga agaaaggccc tacaggcctg gggatcccat 15840ctctgaccac cgaccccagc ccccaactct catagactct gatttgatat tcttatttac 15900caaagaagct gcatgtattt gcatatcaat tctctttccc atatcgacac gcagggaggc 15960ttcttcgttt tggatcaacc agagatcacc aatgtctgca gggtgctcag cccctggtac 16020cttggtcact ctaggcagcc tcctagatgt gcctcttaag ataggttttc tgaaatggga 16080gaggaccacg ccccgccccc caccatggac tctggagtct gcggcgcaca aatcctgcga 16140ttttacagat ggggaaacag aggcacggag aaagtctcag ctttcccacg gcccactggg 16200accagaaccc aggtcgcagg acggggacag ccacctcctt tttctctcct tgcaaccagc 16260ctgagctcgc tcagcgggtg ggggctgccg ctatccagag caccctgttt ctggatgcaa 16320aataaaggcc caggcagtgt ttggccctcc tctgcccaca gactttggct ccagggcagt 16380ctccgagaga gaagccttgg gtcccactgg tccgagctct gcgtgctgag tctagaggct 16440gcagtctcta agccgaaact agcccaggcc tcacagccgc cttcccgccg gtctcccact 16500gccttggcgg ggcgctggcg ccctctgctg gccaagtctc gctctgcgcg caaacgcccc 16560agaaatcagc cggaagttcc ctggggcccc acaggagtga ggacccacct ggattcactt 16620tgtcattttt ttttagctgc atgaccttaa gaaagctaat gaacctcaga gcactacctg 16680tgaagtggaa ataatagcgc ctacacttca gggtcggtgt aaggattgca tagaataccg 16740tatagaaagc acccagcaca ttgtaggtat gtttaatggt agcgattatt aataatgccc 16800aactcacatg gtgagatgag atctgagaag cctccttata aaggtaaatg ataaccaagt 16860cttgttgcaa ggcagctggt gtgttactaa tacatcatga ggtggtgggc cctctgggga 16920ctgtcaggag acagcctctg ttttattagg aaataaaaca gaatctggtc tgctccagtt 16980ttcttccttt tatttactgc attagaaaac tttcatttta tttattgcct attctggggg 17040ttgagggtga ggggaaggca tgactccagc caggtaggat acaaaagtat ctcacccccc 17100aggaggggtt tgaggaccgg ccctccggct tttagagaag ggcagctggc ttctgttgct 17160aacgatttgg atttggcttt taacagaata tcaaggtgaa agtgaaagga gcttttcact 17220ccttggagag tcaagagggt gtggggtggg acctccaggg gaatgaagag tgagcgaggg 17280tgctgggggg atcccagacc taggaatcag atctggggaa ggggtctgca agacccctcc 17340atgagtcaag aaaagccggt gggtggatgg tgagaaggac cagtgaagac actgtttgct 17400ggagttgtcc cagccagtgg ctatgactga gactgtccca tggcgtgtgc ccagggtcct 17460gccattggat gatgggacat tctttttttt tttttttttt ttgagacgga gtctcgctct 17520gtcatccaga gctggagtgc agtggcgcaa tatcaaagtc gctgcagcct ccacctcccg 17580ggttcaagca attctcctgc ctcagcctcc caagtagctg ggattacagg cgtgggccac 17640cacatctggc taatttttgt atttttagta gagatggagt ttctactcca tcaggctggt 17700ctccaacttc tggcctcaag tgatctgctg cctcggcctc ccaaagtgct gggattacag 17760acgtgagtca ctgtgcctgg cctggatgga acattctgct ctttctccat ctgctgccta 17820cccatgatgt cttgggactc ctagaaccct aaaggaagcc cctggacacg caggaaggtg 17880tggagaggag ttctcatact tgcacttggg aggagggctc aggagaaaca gaggctggca 17940acaccccctc acacactggt cctctggagg gccagtgtct acagacactg tggactgagt 18000ccacagagag gaaagggtcc tgccttcatc agaactgctc agcaagcagt tccatcccag 18060ggggtcctgc gaggtaaggg aggggcagct agctaggtga ggggctgaga gagtggggag 18120gggaaagagg gaagaagaga gtgagaggga gagggaggga ctgagccgat tctcagctcc 18180ttgaccgttt gctgagctct gtctgagtgg acagatggtc ccaagtcagg ccacaccaga 18240gtggcctttc tgctccccta caccctgcat tcctcaacat tgctggcccc ggagagactt 18300tccttcagag aagcaaatgg ctggggaatg gtgaaagaca ctgcagagaa aagaaagcac 18360agcctgctgc cctgggaatt aacatgattt aggagacctg caggtcaccc cctcatgact 18420aaaagccatc ctggaatgaa ggtctgtggc tatttctagg caaaactgtc tgataagata 18480aaatagctca actcctgacc attaagtcgt gaaggccatg gccatcgtaa atctcatctt 18540tccggccctc tggcctgcat gcagtgcagc ccagccagtc ggtggcagcc accttggtag 18600gaagggccct catcctcctg gctgtgcccc aaggactggg caggcttcgg tgccaagggt 18660agtgcgagca cttgaaagcc gccctgtatg tttattgttt tccccaggtg atccagaatt 18720actcccgaac tctaccagct gaaatcctcc tcaactcaca tcagacaaga cggccctgcc 18780acttacctgt cagatcactt tgggcaggta agctcatttt cctgaatctt tacttccaca 18840ccttaaaatg tgagcaatac tatctccctg gcaaggttgt ttgtgagggt aaaatgaaac 18900aataatcacg ggtgcatcct ggagctcttt cttacaaggc gtgcccccaa atctgtcccc 18960tctttctgag gatgcccttc ccctattgtc tccctggcca tttcctaccc attctcaagg 19020gccatgatct cagggagttc tcctgactca cccaggcata ttggatctcc tacgtactgc 19080tacgactgca cacaggtgca ggaaatggct gtttgctttg cgtttgagga acttggaaag 19140ggagacgtgg tagggaaagt agtggttagg ggcactttca gactgaagga tgtgggttgg 19200ggaatacggg attcttggag ttgaggacgc cgcttctctc gtctctagct aatgtgagaa 19260agacccttct ggacactaag cctgcaattc cactggtggc taccaggtgt ccgtggtgtc 19320ctggggcggg tgtaatgaga gcgggagcct gtgaaaccaa aagcattgtt tttataaatt 19380cagcactctt caatccctat taataaggtt agcggtgcag ttcttgcgtc tcctgccctg 19440cctcaccttg caatcatatt cattggcatt cctttcttcc aagaacccac ctaggaggcc 19500ttgcaggaga tatctctggt gctggctgtt cctgcagtct gaaaagccag ttaagataca 19560aatatgtgag aggacactgc ttgaatctga tttcattttt taaaaagttt aattattcac 19620agactttgct tctttctgac tagtaatgtg acacctgtga ctcagttcaa gatggtgtgt 19680ggtgaccttg cagttgagaa gcactgagcg ctatagccat gccaagaagg ttgccctcct 19740gggaccagca aaaattccat ccagggggcc atctctgtca ggcttgtcct gactatcttt 19800ttggagaggc ggcagagtgg agtggttaaa agcatgattc tggagcccag ctacctggga 19860gcaaagctcg tctctaccgc ttaccagctc tgagagctta ggcaagtgac ctattctctc 19920tgtgcctctg agttttcatc tgtgaaatgg gagtaacaat agtcctgtct cacagggttg 19980ctgggaggct tgaatgagtt aatgtccatg gggtgctgaa tcagtgcctg gcatacggtg 20040aaggccatgt gagcagtaaa tattattatt attagaaagg ttggctgggc gtggtggctc 20100acacctgtaa tcccagcact ttgggaggct gaggtgggca gattacgagg tcaagagtta 20160gagaccagcc tggccaacat ggtgaaaccc catctctact aaaaatacaa aagacattag 20220ccggtatagt ggtgcgtgct tgtagtccca gctactcagg aggctaaggc agaaggatcg 20280cttgaacccg ggaggcagag gtttcagtga gctgagatca cgccactgca ctccagcctg 20340ggcaacagag tgagactcca tctcaaaaaa aaaaaaaaaa agaaagaaag aaagaaaaaa 20400agaaaaggtc ccaacctact cacttattgt tcacttgcat caaatgctag gttcagtgta 20460ttgttcttgt aagattagaa atggagagat gggtcagaag gggttggtca aaatagacct 20520ctcaacccaa gcagacaggg ctggcttcat ggccgggcaa ccatgcagga cccagggctc 20580taagataaga aaggcacgta gtttaatgct ttgctgtccc atcttgatac tgtttttttt 20640ttcttttttt tttggaaatg gagtttcact attgtcaccc aagataaagt acaatggcgt 20700gatctcagct cactgcaatc tctgcctcct gggttcaagc aattctcctg cttcagctcc 20760ttgagtagct gggattacag gcatgtacca ccacactcag ctaatttttg tatttttagt 20820agagacgggg tttcaccatg ttggccaggc tggctggtct cgaactcctg atcgcaggtg 20880atccacccac ctcggcccca caaagtgctg ggattacagg catgaaccac cgtgcctagc 20940tgatattctt aatttatgaa ggaagggccc caaattttca ttttgcactg ggcccacaaa 21000taacgtagca ggtcccacaa acaaattcgt ctagattcag agggcccctt gccttcctcc 21060tctgctcaca ttcgttcctt tctcccatca caggcgggta ccctaccttg ggggatttgc 21120cccagaataa gccttttttt tcccttctaa cattttaatg aaaaatttca agtgtatagc 21180aatgttgaaa agattttata gtgagcaccc atacacctgc cacccaaagt ctaccattaa 21240tgtcttctgg acttactttt gccgtcttat tcatactctg cctggactgt cctcagctac 21300aggactcaca tctcttgccg acagctctaa ggcttccagt cctgctgtct ggaccaagaa 21360aggcttcctg ggctctgagt gtcaaatggc ggccttcaag gaaggggaat ggtggaaaag 21420gccgtggggg gttttggaga aattgctagg gaaagactgg caccagagtt ccaccagccc 21480aggcaatggg gggtacagaa cccataagat gagttctaga aaagcaagga aggtttcgtt 21540ctggagtttg tggactgagg tttccatttg tgactaggat tctcattggt tccttatgta 21600gtttcttacg ccctgcatag tcttctaagc atttctcaca gacgtggtct gggggcgaac 21660accagcagcc ctgagaggtg gctaaagagg agattcttct ctccacttca catcgtagga 21720aactgagtct cagaggttcc ttccctggcc tgcccacaac cccagggcta aaagaggcag 21780accaagccca gggccttgaa ccccaacaat gggcctcttt cttttgatcc catgataggg 21840gtgcaaaagc attgcattcc cctgggtaat ttgaagaaaa aacccaaaaa actccaactt 21900tgtctccagg aaaaagaggg tgtctgggct atgatttacc tctgagggtg tggttgcact 21960gagcgtgatc acacttcaaa gggttagatc tcatttctct gcctttctag cttgggccca 22020gggctcagaa atgtgtggac tccctcacag cccctcccag catccctgcc ccctcccaac 22080tgccttgggc aggtgacacc tgtattattg ctaagggtta aaaagccccc aaatcaataa 22140aacccattaa tgagtgttgg tacctcgaag gctacagata aatcccttct actcagtgag 22200ttcaatccca taaaacagct ctcccctttc aatcctagca ttcatttgat agaaaatgtg 22260gagaaatttt aaaaaggtga cttactaatt gcctgtaaaa taaaaggcag atggaagctt 22320tattacagtt gaaggaagtc gggaatatta aggtaaaatg tcaaataaca attgattttc 22380cttagacata aaggggcgat ttatggcttc ctagttacta caaacgagaa attatttgaa 22440gttctgaaaa gtatgaggag aaataaagat taaatagaag atgaaatcat agggatttct 22500ctgggaggtg acttcagtgc ccctggggac tagaattcat gtggccagtg gcctagccag 22560ctggggcttg gcagtttcaa gatttagagg caaggtgtct ctgaggagcg gggaagtggc 22620tgtttgcttt gtgtctgagg aactaggaaa gaaagatgaa gatagggaaa gttgtggtta 22680gggccaattt cagactgagg gatgtgggat taggggattc ttgtggatgg gcctatagct 22740ctgcttcctg actagcagat actggggatc tggggaaggg aagggtgagc tgcctttcct 22800gggacttcga tggcatcctt acagtcaggg gacactgtcc ttgctgggtc ccggacatca 22860gtgtctatgt atccctgcaa gccacagcac tatccaggcc ctctggtggc tttgcttggt 22920ctgggccaac ctggtctcca cactgacagt caaagacgag gagaggaaaa agaactcacg 22980gctaggcgtg gtggctcctg cctgtaattc cagcactttg agaggccgag gcgggtggat 23040cccttgagcc caggagtttg agaccagcct gggcaacatg gcgaaatccc cgtctctaca 23100aaaaatagag aacaattacc caggaatggt ggtactctgg aggttgaggt gggaagatgg 23160cttgagctcg ggaggttgag gctacagtga gctgtgattg tgccaccgca ctccagcatg 23220ggcgacagag caagacactg tctcaaaaaa aaggctcatt tccaggctgc caggcttatg 23280ttagcctggg gtcccgcagg agctgacctg agacaagtac ttgagggcaa gttgtttgtt 23340tgagggatgc tcacaggaag ctccagtagg agggtaggga ggtgacccag ggaaggaagg 23400cagcttacag gggcgtgttg tcaggaaggg caccccggcg ggtgacggaa gcttaatttt 23460gctgggaaac tcaggagcca gcatggaacc tgcacctcag agttatccca ctggaaggat 23520gagggagcgg gtacttatac accaactcca tccccgtcct ttgttaaggc tgctggatgg 23580agtgggtggg acactcattc ttcagcactc ccaggggccc tcaggctgtc agaagttagg 23640tccgttgggc tccaggatga ggggaccccc agaagatgcg ggagggcgtc cgcagcatct 23700gcctctgtgc tttccctgtt tatctgaatc ctcataattc ccgccacatg cagatcagag 23760cccccagctt tatggaagag aacacaggtt tggagaagat aaagagcatt cccacaacta 23820ggaggtgggg aaagctagga gttcagccca gagctccctg acttcaaagt ccattctctt 23880tctacttcct gatttttttt tttttttttt ttttttgaga cggagtttca ctctgttgtc 23940taggctgggg tgcagtggca caaacttggc tcactgcagt ctctgcctcc tgggttcaag 24000caattctcct gcctcagcct ctcgagtagc tgggactaca ggcgcccgct accatgcctg 24060gctaattttt tgtgttttta atagagacag ggtttcaccg tgttggccag gctggtctcg 24120aactcctgac ctcaggtgat ccgcttgcct tggcctccca aagtgctggg attacaagca 24180cgagccactg tgctcggcct acttcctgat ttttgtataa gacacatccc agcagcatgg 24240tagactgaag actcctgggc ccactctcag agattctgct ctggcaagga tgtgtttatt 24300ggtgagaggg gtgtccagga atacgtgcct tttttttttt ttctagagac agggtctcgt 24360tgtccaggct ggagtgcagt ggtgcaatca tagcttactg caatctcaaa cttctgggtt 24420cgagcgatcc tcctatctca gcctcctaag tagctgggac tacaggtgca cgccaccatg 24480tctggctaat ttttaaattt tttctggagt tggggtcttg tgatgttgcc caggctgatc 24540tttaacttca ggtctcaaga gatcctccca ccttggcctc ccaaattttt gggattacag 24600ccatgagcca ctatgcccaa ctagaatatg cacttttttt tttttgagac cgagttttgc 24660tcttgttgcc caggctggag tataatggtg cgatctcggc tcaccgcaac ctccccctcc 24720caggtccaag cgattcttct gcctcagcct cctgaggagc tgggattaca ggcatgtgcc 24780accataccag gctaattttg tatttttagt agagatgggg tttcttcgtg ttggtcaggc 24840tggtcttgaa ctcctgacct caggtgatca gccccgcctg ctttggcctc ccaaagtgct 24900aggattacag gcatgagcca ccaagcctga cctttttttt tttttttgag acagagtctt 24960gctttgtcac ccaggctgga gtgcagtggc acgatctcgg ctcactgcaa gctctgcctc 25020ccaggttcat gccattctcc tgcctcaggc tcccaagtag ctgggactac aggcacttgc 25080caccacgccc ggctaatttt ttgtattttt agtagagatg gggtttcacc gtgttagcca 25140ggatggtctt gatttcctga cctcgtgatc cacctgcctt ggcctcccaa agtgctggga 25200ttataggcgt gagccaccgt gcccggcctt tttttttttt tttttttttt ttgagacagg 25260atcttgcttg gttgcccagg ctggagtgca gtggcctgat tacagctcac tgcagcctca 25320atctcctgga ctcaagcaat cctctcacct cagcctcttg agtagctggg accacaggtg 25380tgtgctacca cacccagata atttttgtgc tttttgtaga gacagggttt cgacatgttg 25440cccaggccgg actaaaattc ctgggctcaa gtgatcctcc tgcctcagct tcccaaagtg 25500ctgggattac aggtatgtgc catcgtcacc agccagaata tgcattttct ttttttcaga 25560cggggtctca ctctgttgcc caggctggag tgcagtggtg tgatctcagc tcactgcaac 25620ctctgccttc tgagttcaag cgattctcct gcctcagcct cccaagcagc taggattaca 25680ggtgtctgcc acaacacccg gctaattttt gcatttttag taaagatggg gtttcaccat 25740gttggtcagg ctgatcacaa actcccgact gcaagtgatc cgcttacctc ggcctcccaa 25800agtgttagga ttacaggcat gagccattgt gcctggccga atatgcactt ttaataagca 25860tcagccaggc taggcaggcc gggggccaca ctcgagaaca tttgcaccac agccactggc 25920tacctgcccc tttttccata aggttccact gccctctctc ccctctatct gggtctgttc 25980ttcaggttct tccctgggag ctctctgagt gacataactg tccccaagtg ctgggagatg 26040gagagaggaa tcaccagact ggagcaggcc cccagagcgg agatgggaag gggaggctgg 26100tgttctgagg ctcccgaggc agtgagaggt gaccggaggc agtgagaggt gaccgaagac 26160agtggctgag aaccagggag gggctgcggg aaaaagccct gggtgcaagt cgctctttcc 26220ttagcgtctt tgagaggagg gatggggaaa ggtgaggtac tagggaaaac catctggaag 26280gaggtcaggc tgcagaaaag ctgcaggagt ctgggggact aagaaaacag tgggagaccc 26340cgctgcagcc cagcacgtga gggtgagaac gtcatgaatg agggaagagc aggcaggggg 26400tggtggcctt gtggcctctg gagaggagga gccacatgac tctggggtga tctggggtga 26460ccctcagggt aagggtacct ccctagcact gagtacagag ggaagcctac actgccaggt 26520gcagtttccc tggcaatgct tctccttcta acactatgtg agtttcctag tgctgctgta 26580acaaactgcc ataaaccaag tggcttcaga gaacacagac ggactatgac agttgtggag 26640ggaagaagtt tgaaaagcgg ggtgcccgca ggctgcagtg cttctgcagg ctccagggag 26700aatctggtcc ttgcctcttc cagcctctag agcctgccgc actccttggc tcatggcccc 26760gtgtcattgt agcctctgct tctgccatca catctcctcc tctgcctctc ctgcctccct 26820ctgtccttta taagaacgct tttgagtata ttggacccat ctggatagtc caggataaat 26880ccctccatcc tcatatcctt aatttaatca cacctggaag gttccctttg ccacataagg 26940taacatattc acattgggac agttagggga ttgggatgtg gacatctttt gaggtgggaa 27000gaaggagctg gggttctttt taacattttt ttgagacagg gtctctgtca cctaggctgg 27060agtgcagtgg tgtgattgag actcactgca gcagcctcaa cctcctgggc tcaagtgatc 27120ctcccacctc agcttcctga gtagctggga ctacaggtgt gcaccaccat gcccagatga 27180tttttgtatt attttaatat tttgtacaca tggaggtctt ttaatgctgc ccaggctgga 27240ctcaaactcc tgggctcaag caatcctccc gtcttagcct cccaaagtgc tgggattaca 27300ggtgtgtgcc accagaccca gcccggacct ggggttcctg tctcatgtca gccttagcaa 27360tctgggtgac cctgggcaag gcctatcccc tctctgggcc tttgtttctc cacctgtgca 27420atgaggctgt tccctctggc tccttcactc tgagtttttc agttgggaga atatcttggc 27480agggagcaga ggtcggcggg ggtggttgtc attccatttc agggcctctc agagtcctgc 27540cgtggtgtgc actgtgtgtg tgtttaattt tctacatttg gatgtgatcc taatccaata 27600aatgcttagg agacttctat agaatagatt aatttttact agaaaaaaat ataattggct 27660gatgttaagg ctactgccct gacaaatctg ccttggccat atatctgaga aggtaaaaga 27720cccgctacgc ttgcacataa atatgccatc ttccccacag gccctggaga agcaccccgg 27780ggaggtttcc cttggtgatt tattcttcat taataagctc tatgctatat taggatcaga 27840tttatgactc tgcctttcta atatttctga catttcatct gaaaagaatt acaaatgaaa 27900tcttgaaact ttgccacttc tccctgctag tgctctggca ctctgtgtcc aaggggagat 27960ggtgggctgg ggagacccca agaagcaggg acagaggcat gtttctcagg gaaaggagcg 28020atcagcttga ctttgggaga gctttattca gtttgcaagc agcttgggag gtgagcggtt 28080caggcgagaa ggctgcagac cagaccacaa gcccagcagc agcagtgatg cctgtaacat 28140gtatgagatg gtggcaggca cattcattca ttcaacagct atttgttaag cactttactg 28200tgtgccaaac actgtgctgc ttggtgcttg ggatacagga aagaataaaa gtgaagcagt 28260gatgaagatc ttggccttca tgcatatggt gttctagcag aggtagggga gtgtcaagca 28320ctgatgaatg aaaatcctga ataggtaaaa tatatcatat gttcacacgt ggtaagggct 28380atggcaaaac agaaacaaac cctccacaca gggaaaaggg aaccaagagt gccgggtggt 28440caagttgcaa tgttaattaa gaaagcactt caactcatca actcaaggaa ccgtattatc 28500cccattttac agatgaggac ggaggctcag cgtggattag gagctacaca aagacacaaa 28560gggaactttg cacaacttga aagtttgcaa agtgccgtct gagccattag tctcccttct 28620ccccactgac tgccctacca caatcacatg gggaccataa aaatactgtt gcctgggtcc 28680cgctcccctg aggctttgat tcaattggtt tggatgcagc ctgggacttg gcatgtgact 28740cttgtggtgc caggactaag aatcttgtgt gaactcccag cacagcctct caggcctcta 28800ttttctcacc tgcaagatgt gggtaaaaat attcaccctg tactgcaaga ggatgtcagg 28860agtgaaggca gaaacacagc accagccctc caggaccccc aacccctccc ccaccccaat 28920ccttcacccc tgttgtacct tctgacctca aagtggctct gattatttca ctcccacagg 28980ccactggctc agaggtatag agctcacctg tggcagatgg agatgcggat ctgaggcttc 29040tgatgctgcc acacccagcg gcgcccccca aattccgggc ccctggatga catctggtct 29100gttcctgcag catcagagca caatagagcc agccaccagt cccagccctg cctgcatccc 29160atccattcct gggtgcctaa ccccgaggat cccctggcag tatgatgcgg acctgtcttg 29220gatcccaggg atatgctggc cacggggagg agccggaaac caacctttgt gtcactgtgt 29280agtgacaagt gcctttggag gtcacaatag ccagtggtga tttctaccac tgcccccagc 29340agccaaggtg gcagaggagc cctgtcagtc acccccattc tgttcatggt ctcacggtgg 29400gctccacatg gggggtggca gccctctccc ccaccccacc cgaccccttt cgacagatag 29460ggtaatacaa atacaaataa caccaaaaga ttgagttgct gggcagaaag ggaccaaagg 29520ccagtgtgtg tgtgaggggt gggggcaggg caggagagga gcagcaaaag gctgtgaccg 29580cctggctgag cactggatac tcactgaagg gcagggaggc ttcctggaga aggagacctg 29640gcaggggctg agggagtgat gccaggcatg ggggtttgga gggaccccag gcatggcatg 29700cctccattcc tccctgtgct atccactcta tataaggggt gctgtgcagg gagacagctt 29760gcatccaagc agggaggcag ggaggatgag aggcagagag gagcccagct gggttgatgg 29820aaagtctggg aaatgcagga aatccaggag ggggagaatg attccaagct gtggcctgtg 29880atgggccttg aaaccaggtg taggcacttg gatctgatcg ctggggagcc agagctgctt 29940cctgagcagc agaagggcag gatgcgaatc agactagggg cagtgggagg aactgagagg 30000cctcaggtca ccggagaaaa tgcacagggc cgggaggcag agatgctcct gttttcttgc

30060tctggggctc aggacagtca gtcaccctga gcttcagact cagctcactc attttgcaga 30120gatcctgacg gcgatgcttc aggatgatct gggaagagtc aatgaggtaa aatatgtgaa 30180atatgccttg aaaactacaa accacagcac atgttctgtt tttgcttctg cttgatggac 30240tcagtgagat ggtggggaca agaattagag aagcccatga ggaggccaag gggcaccaaa 30300tagacccacc aaggaccagt ggggacttag agaaaggatg agtcagagag aaatgacagg 30360agcagaaggc aggccttgta tggaggatga aggtgaagat catacaccat taaacttgag 30420aaagaggcgg gaggagctgc catttcttga gtctactgga tgccagcagt agtgctgggc 30480aagggcttaa caggtgggga aatcgaggca cagagaggtt aagtagcttg cctaagatca 30540cccagttagt aagtagcaga gcctggcagc ttaactccaa agtctttgta ctaaagccag 30600attttccaaa tttgcccaac tgtaagaatc acctgggcct gtaatcctag cattttggga 30660ggctgaggtg ggtggatcac ctgaggtcag gagtttgaga ccagcctggc caacatggtg 30720aaaccctgtc tctactaaaa atacaaacaa ttagccgggc gtggtggcag gtgcctgtaa 30780tcctgtaatc ccagctactt gggaggctga ggcaggagaa ttgcttgaac cccaaaggtg 30840gaggttgcag tgagctgaga ttgcgccatc gcactccagc ctgggcaaca agagagaaac 30900gccatctcaa aaaaataaaa gcccgggagt ttattacaga tgcatattcc caggcacctc 30960ctacggaggt tttgagttag tgagtccaag gccttctgcc tcttcccaat gtattcatta 31020tgcaccatca ttactcttgt tcagatacaa tgtgagtgat agcttgtctc tggcagcaca 31080gcagccacac ccaaccaatc cagacctcag tcatgagggt gccaatcaca gctaacattt 31140ttttttttga gacggagtct tgctctgtcg cccaggctgg agtgcagtgg cacgatcttg 31200gttcactgca gcctctgcct cctgggttca agcgattctc ctgcctcagc ctcctaagta 31260gctgggatta caggcacctg ccaccatgcc cggctaattg tatttttagt agagatgggg 31320gtttcactat gttggccagg ctggccttga actcctgacc ttgtgagcca cctgcctcag 31380cttcccaaag tgctgggatt acaggtgtga gccaccgcac ctggcccaca tagctaacat 31440ttaatcagca catacagggc catgctcatc attttttgcg cacaatgcca tttaaccttc 31500acaacagcct gtgagaaggt gtgttagcct cattttacag aggaagaaac taaggcccag 31560agaagttatg caacttgccc aaggacacac agcttgaagg agctgaggtt taacccgttt 31620ctacggggtc tgaatcctcc ttaccacccc tatctcccct gactcccagg ttgtgtttgg 31680tgtacttggg tagtgtccag ctgacaaatg agatgtttta gcttcagaca gtctatgcca 31740tgtagaaatg cacccaggac tgtgcatatt agggaggttt gcaaatgtgt ccacattaga 31800taatttttct caaaatgccc ctcactgaac ttctgtcatg cagtatgtac tgagcacctg 31860cctcttgcta ggtcccggga gagaggatga agagggcaga gcccccaccc tcaggggacc 31920tgaaaaatgg gagtcctttg tgctcatgga agcagggcct tggggcctca gcactatgga 31980catttggggc cgggtaattc tttgttgtgg ggggctgtcc tgtagattgc aggatgctcc 32040gccgcatccc ttgcccctgc cctctagaag ccagtagcac cctccagttg tgaaagccgt 32100aaaatgtctc cagacattgc tcccagttga gagccacttc attaaagaaa aaaaaataaa 32160aaagatttct gtatcctcta tacatggact gaatttcact gacatttctc taaaataact 32220gaatctattt ctatactcat ttttttttac cagctgccat tttatttatt tttcttttct 32280ttcttttttt aagaaacagg gtctcactct gtcacccagg ctggagtgca gtggcgcaat 32340tatggctcac tgcagcctcg acctcctggg ctcaagcaat cctcccacct tagcgtcccc 32400agtagctggc acgccactgt gcccggctat ttattttatt ttattttatt ttttttgtgg 32460agacagggtc tcccaatgtt gttcaggctg gtctcaaact cctgggttta agcaattctt 32520ctgccttcat ttcccagagt gttaggattg caggcgtgag ccactgcacc cggccatttt 32580atttcttaac aaagcacaaa tcaaatgtac aatgcagcta gattttcctt tataaataat 32640gtctaatgga tttgtttctg tcagctgcct agaacattct ggtatcccag acagaagtgc 32700aacaccaggt ggaggtgttg cagctgagaa gttctgacca gcataccagc acccccttaa 32760aagctgtcta tacttgcggt tctcagctag gctgccacag aatcgcccaa gtcttaaaaa 32820aacaggtctc tgtcctactg cttgagatgc tggttctcta ggtctgaggt tctggcacct 32880gtgtgttttt aaaactttac aaataattta aaagcgcctt caagtccaca acaacagaat 32940atttccctcc actcctcatt gtcctggagt tctctaacag tgcagttcta caactggaca 33000cacgatgtcg cttttcagcc acagttctca ctaagcggcc ccacagggcg gcaggtgcct 33060tctgcagaga gagagagagg gccttggctg acaggccaag accgggcatc ctggctcctc 33120ctctgtacag acttttcaca gatgtgaact ctccctactc cctgtcttct gcccccaaat 33180gaagcctctc agctggcaag agctgagaac taccaagcga gccattgcta atttctattg 33240tgtttggaac cacaaaaggc agaattatta aggctgtaaa ggacctcaga gcatctggtg 33300cagtgagttt ccaacttgtg aaaatctgat gtgatctcgg acaagtcact taaccccctg 33360cctcaatttc ttcatctgta aaataggata ggaatatatc ttgcccgtct gattgttatg 33420aagacaaaga gaacaaatgc acataaagcc cgtgaccatg tgctttgtga atggaagctt 33480taatttattc attcatttat tttattattt atttttattt atttatttat ttttgagaca 33540tagtctcgct ctgttgccca ggctggagtg caatggtgcg acctcggctc actgcaacct 33600ccatctcccg gattcaaaca attctcctgc ctcagccttc tgagtagctg ggattacagg 33660tgcctgtcac catggccagc tagtttttgt atttttagta gagactgggt ttcaccatgt 33720tggccagggt ggtctcaaac tcctgacctc gtgatctgcc cgctttggcc tcccaaagga 33780agcttttatt tttattatag ttttacataa ggataaattc agccttagtg aagggaagtg 33840acttgcccaa gatcatacag tgagactgct ggatctgggg ctctacttca gaattttttt 33900ttgagatgga gtttcactct gttgcccagg ctggagtgca gtggcacaat ctcggctcac 33960tgcaacctcc acctcccggg ttcaagcgat tctcctgcct cagcatccct gatagctggg 34020actacaggct cccaccacca cgcccagcta atttttatat tttgagtaga gacagggttt 34080caccatgttg gccaggctgg tctcaacctg cctggcctcc cagagtgctg agattacagg 34140tgtgagccac cacgccagtc tactgccatt gtccatgatt tttcctacag ggaaaatcac 34200aatcccagaa gataagacaa agaacagtaa aaggtggctt ttgaggcagt gagttctacc 34260tgaaggtggg aacagcccag agtgtctggg gacagagtgg taaattctaa tcaagccttc 34320ccatggcttt gtggatgagg atgagtttct accctgaagc tcggcctgtt cagccataaa 34380atcaggataa tggtggctgt gcctccttag agtagaatga gaatcagagc agaacaaggg 34440aaagctgcag tgacttgtca ggtgtcaacc ttcagcatga tgggagagcc atgggaccct 34500tcccctttcc ctaagagagc cagccctcac agcaggcctg ggatccaatg cccagcaccc 34560agctgggagc ccagggacct tggccaaaat ctggttctgc ttcaacttgg tgcccggcct 34620ttgtcaagtc acttcctcat ttgcaaactg ggagagtttg gatgaaacta ttgaatgaaa 34680ttattttggg ggtttctttc tggctctcac agtcctcgca tgctcaccat gttcccttca 34740atttcattag cacagcccaa caaagggtta agcagtggca gttcctctcg ttctttggtt 34800aggacaggaa ggtcaggggt gaggccaata caagaggtag ccgccacagc tgatgctgga 34860aatgacaata gttctttcct agactcatat ttgtcccctc tccctgaagc tttgcctgca 34920gtgcccttgt aaagaagttg gcaagaagca ggagtgaggc tcagcccctc tctgaaatgg 34980atacgccggt tgctccccct catggctggt ctcatttgcc ttcttcattt ttagacacat 35040tccaaacttt tcagcaaatt atagtgtttg ccaactggcc gtctggggcc caggagagat 35100gctatttata gcgatgctgg gatgctgcca tcccagagca gcctggtaag aaacggagcc 35160agagtgcctg ggagtggcgt cctgcaccct ggggagaggc cagggccctg gagcagggtg 35220gcaaagctgg tggcccgtgg caaggaccac tggcacatcc cctgcctgcc tgggccctgg 35280ggtctgtgcc cataccccac acggggggct gcttccgtgc tccttggaga gacgatggtg 35340ctgtggggcc actgagcaca gtaaaggcta agacccacca taggtcagcc cttgctcatg 35400ctgactgttg ccccatttcc cttcattctc tcactcgttc attcctcaga atctgcatcc 35460tggttttgtc actacctgga gttgtaaaga taccaccaag ctcaccttgt ggtgtgagcc 35520ttgattttcc ccatctgtgt aaagggtgga tctgagctcc aaagttcctt ctagtcatat 35580gcagagtgca taataaatgt gtttgtattc ccactgtgct cagtgggcac tggggcatgc 35640agaagagaaa ttggaataaa tgtgaccctt gtcctccaga ggctcagtac cagactggaa 35700ccaggaccca gatgaggggc ctacccagag agggcagcgt gctctcctat ttgtgttagg 35760cgttaccatt tacaaagggc tttacggctt tgaaggtccc cacaacgccc tgaggaggtg 35820aagtgtggca atgcccgttc acttgggaaa atactgcatc tcagagagac caagggactc 35880gcttaaggtc acacagatac agtaagtaag tagggaaaga gctggggcct ctgggctcct 35940tttccggggc tctggcctcg gttttaaccc gttctcctag acctctcagt ctctgggcct 36000cccctctgtg ttccccaccc ccactttcca tcaagagctc agttctctta agttctatat 36060tctctcttcc ccacccctag aaatctctgc ccgcttccag aaaaggcttt gattccgtca 36120tttttggcat ttcccaccca ggaatcaagg ctgcctcctc tgtgaggaag gcgtgtggaa 36180ggcgagcagc tgaggacacc tctttttaag agaagcttct ccatgttctt agccaggtta 36240tataacttct tttgcttctt tcttttccac attctacata tttttcacaa tgaggatgtg 36300tcacttctat agttaaaaat ggaaatcttc atttaaaaga acaatcagac acaaggcaag 36360gtatgcttac cttcccaatg accaaggagc agggagacat tggtcgtgga ggccataggt 36420gaccagcctt gagggaaggg aaggaagggg aggtgcaggg aggcgcagca gaggcacccg 36480agtgtgctct acgaatgtaa gtctgccagc tgctcctctg tgcctagcac cctggaaagc 36540gcccgcacac agtgggccct cagcaaactc ccactgagca aagggccctg tgagtaagga 36600cctagaagca gggtgtgctt gaggcatggg gtgggagggc atccagcttg gctggcggtg 36660ggagccgatg caaaggtgag ctgagaccag acggtagaag accttcagtg ctgggccgag 36720gagggttcct tcatcccata gagaagagct gccatccaag acagaaggct ggggagtgac 36780atgttgaaat cagtatctta ggaaaataaa gccactggct gggccactgg ctcccctttg 36840tagaggggtc tacgaagctc ccgaggtttc agatagtccc tgagagccct tcccctcgcg 36900atgggctccg tttgaggcat gtccagtgtg aaaggaccac aggccacctg agggacagga 36960ccaagcagag agctagtgac agaatgccca gggctccaag acagggctgg agaggtggga 37020gtggtcccca ccttgagagc caaaggggct gagggctgag ggttgaaggc cgagagccag 37080gaaggtccag gggagcaaaa gggggagcag agggggagct ggaggtgttg gggtggtggg 37140tagagagcca agatagggag gggacaagag aggggagaat ctcaaggaaa agcaggagca 37200tgagactgag agaaggcccc tggaacactg gcttgaatgt ggctgacatt ggcactgcca 37260gtactcatcc caaaccaggg agctcagcca gcgccctttc tgcatgtccg ctctttcatc 37320agacagctag gaacaagtta tacgagatga tgtccaagtc caataaatgt ctaagtcctt 37380tccactggat ctggccccag cctcctctct gaaccatctc ctatcactcc accctatccc 37440tgctggctcc ttctgctcca gccatacagg cttgctcctg ggctcagctt gtgcctgcct 37500ggggagcctc gcgcttgctg tttcctcctt gagaaattcc tgccctggat aactccttcc 37560cccacttcct tcagatctct gctcccttat cagggggctt cccctggctg ccctatctat 37620aacagccctg accactcttt tcctcttctc ctgctttctt tgtcttcctg ttcttatcac 37680taagacatca cacatgtctt agttggcttg tttatattct gtctccaccc atcttagtca 37740gctactgctg aaatgatgct gtgtaacaaa ccaccccgat actcagaggc tccaaacaag 37800tgctgatctt tctcatttgt gagtctgtgt gtcagcccga gcagggcagc tccaggctat 37860ggcttgggtt tgggtggact ccatgtctct ctccattctc cttggaccag cagctccctg 37920gggcacatgc ttgtcttgga gaatggcagg aacttaaaag ccaagccaaa cctcacagca 37980cggttaaggg tgatcatgac acaccgctaa cattctagtg gccaaagaag tctcatggcc 38040aagtgtaata gcagtggatt gggcaagttt actctcttac acctgttgtg aaggggagtg 38100ggaggggatg ggggggtgac cgttttcaca acaacagtgc aaactatccc atctcccaat 38160agaacataaa caccttaagg ttggaactgg gtctgtatcc ccagcactta aaatagtttt 38220tggcatacag taagggtgca atagacattt gttaaataaa tatacagact aaccaattag 38280cataatacag atgacaaagg tgtctctctt ctgccagctt ctgtgcctga aatgttgatg 38340atctcctccc tatcttccta acatctctcc tgtccccacc tgtccatgcc cagggtttgg 38400ctgctgtgac agaagtgtga gagcctcctg tctcccttgg gatcccagta agagcttcca 38460tgcctctctc cctgctcacc tgggctccca tccctgggga ccttctggaa acagcttcca 38520gggctcccag agcttactta gccagattct acatctggct ccagctgtta tcctaagctt 38580ggccttgttt tctgatctga ccacagcttc atcactccta cctgactgtg ggattctagc 38640ccccagtggg gtgggggacc aagggtgaca attacctggg accttgacta ttgaaaggct 38700aatcaggttt gattgggaaa aaagagttgc taaaaaggat tgtattggat aattggtgag 38760atttgaacat ggattttata ttagtattgt gttattacat tttctgatat tagtcattct 38820tttttttttt tttttttgac ggagtttgct ctgtcaccag gctggagtgc aatagtgtga 38880tctcggctca ctgcaacctc tgccacccgg gttcaagcga ttcccctgct tcggcctcct 38940gagtagctgg gactacaggc gggtgccacc acacccagct tgtttttgta cttttagtag 39000agacagggtt tcaacatgtt ggccaggatg gtctcgatct cttgacctcg tgatctgccc 39060gcctcagcct cccaaagtgc tgggattaca ggcatgagtc accgctcccg gccgttagtc 39120attcttatag gtatgtaaga aaatgtcctt gaggtattta gcagggaagt tcatgatctc 39180tgcagcttac tcacaaatgg ttaaacagaa tgagactaag aacagactag taagaaaaga 39240ctgaagaata taattattag gccaggtgca gtgactcaca cctgtaaatc ccaacatttt 39300gggaagctga gataggagga tcacttgagg ccaggagttt gagaccagcc tgggcaacat 39360agtgagaccc catctctaca aaaaaaaaaa aaagtaaaaa ttacgtgggc acggtggtat 39420acgcctgtac ttccagcttc ttgggaggct gaagtgggat gatcagagga cctcaggagt 39480ttgaggctgc agtgagctat gattgcagca ctgcactcca gccagggaga cagactgagg 39540cccggtctct aaaaaaaaag gaatctatgt gagtctatat attcgtgcaa cttttctata 39600ggattgaaac tctttaaaat aagcttaata ataaaaacaa aagctggtgg gtgagacctt 39660tcatgtactc tccaggagag ttaagccccc caacattcct gtccccttgt ttactctcaa 39720gcaccccctc ccccacccaa ggaccaggtc tttgtttact gagcatctca gcgatgagct 39780ctcaccccct gatttcatca attataaatg tgctcgctac tcaccacacg gcaatttgtg 39840acggactgtg gtttgtggtg agagtagcac catccaagtt caccgcagcc gcgagtagag 39900atgagggttg gggccagaca cagggctgtg ggggcggcaa gggcacgcag gcagccctgc 39960caccttcctg tttgtcagcc aagtgaggct tccgagggca gcgggcgagc gggtcactac 40020tcagggccag ctactgcggc caggccaggc tagtcaggtc tgtgcagcca gaactagagg 40080ctccgccagg atgtgaggtc tcccagctcc tgggaactga agcaaacact ctgactaccc 40140cttcttgaag tgccttacgg tgtatacatt tgtttaatct gcacaacaaa cctaggagca 40200gttattgtta ctgtcctcat tttgcagatg aggaaactga ggcaaagaga aattaagtaa 40260ctccttcaaa gtctggtaag tgacagaacc agattttacc ctcttactca ttgtccatat 40320tgccgagtaa cttacattaa tagatactat gcatgtttat tttatttttt attttttttt 40380agagatgggg tctcattctt tttttttttt ttttttgaga cggagtctcg ctctgtcgcc 40440caggctggag tccagtgggg cgtgatcttg gctcactgca agctccgcct ccctggttca 40500ctccattctc ctgcctcagc ctcccgagta gctgggacta caggcacctg ccaccacgcc 40560cggctaattt tttgtatatt tagtagagtc ggggtttcac cgcgttacca gtatggtctc 40620gatctcccga actcgtgatc cgcccacctc ggcctcccaa agtgctggga ttacaggcgt 40680gagccaccgc gcccggtcct cattctttca ctcagactgg agtgccgtcg tgcaatctca 40740gctcactgca gcctcaactc ggggctcaag caatcctgcc acctcagcct cccgagtagc 40800tgggaccaca ggcagtgcca ccacacccaa ctaatttttg cattttttgt agagaccggc 40860ttttgccatg ttgcccaggc tggtctcgaa ctcctgagct cagacaatcc acttgcctcg 40920gcctccaaaa gtgctgggat tatagcacca cgcccagccg atactatgca tgtttaactg 40980acacctaatg attaggtagc aacaatttct tggctgcctt attcaccttc atgaccccca 41040acattgaaaa tgtcttttcc catgtgaaag catttgtggg ctcctactgc caatctataa 41100agtagaaatc cttctgtggt acaaagccct ctttcttatc taatttccca tttcccgttc 41160ccccttcccc cttctgctaa gagctcttcc ctacccacat tgtaagtagg gccggggagc 41220taactccacc ttgcgtactc caaatcaacc acatgaccca ggcctgacca atcagggctc 41280caaatcctct agctatagtg attggtttag ggagggacat gtgacccgcc tgagccaatg 41340aggatctgtt ctgggactcc tgtttgaact cttgggaaaa taaactcctt atgttgggtg 41400gctgagggga tgaatgtgag ctgggagctg caagcaggca tcatgctatt cccttcagct 41460ttcaagtagt gttttcactg ctgttaacaa ttccaaatct taagtgttcc ttgcatcttc 41520ctctcgccaa aaatattaca gtactggagg gcttacatgg tgtctcaaat gtctggagtt 41580taaatctgcc ttgcagcttc catggccaca gtgagtgtct gagtcctggc cgctttctgc 41640tgatcccggg gagagtttag cctgcctgcc cttcctccct ggttcgttct catgtactca 41700gatccctgcc tcactcttct ctcacacaga caacaaaaga gagtcagatt gtggtcttaa 41760gtgcacagga ctctgaagtt aggaccagaa gaccttggtt agagtctaaa ccttgtcagt 41820taccaaatat cattaggcac attagttaat ctctctgagg cttatttccc catcagtaaa 41880gtgggtactg ctgaagatgt agtctttaga agtgttctct agattgtaag ccccgtgctt 41940cctggttatt gactaaacag ggataaaaat gagtttctcc tcacctgggt gtcattgagt 42000ttctggtcat tgtgagatgg gtgatgaagc caagggcatc aaacatccca acgaggctgt 42060tttctgcatc tccagggcta tcccagcgtt aatcacatat ggacctttaa tcagagtttg 42120cagaataagt gagaattgtg ttagagtatg aatgtagata ttatcttgtt ttagtcattg 42180ttactctagg ggaccattct tactacagga atattgcaca aaacccagaa atttattgac 42240tttctcctaa ccaaggccta aagagctggt gttagacata ggccaaggcc agccagaggc 42300ccaaagcctg tttcccaggg taggactgcc ctggcctccc cctctttctc cccaggctcc 42360accccagaga gctgaagacc aggctgggta cggcactgct gagaaactga ggaaaaggcc 42420actggcctcc tctctcactg caggctgccc acccgggagg gggaaagctt gtcactaaat 42480caggttcagt tttggtcact gtcttggact ggatattcta gcatcagaac tgagatgttt 42540cttgtgactt aaagtaactt caggactcta ttctacctag gattgggcag aaaagttatg 42600ggcctgcggg agttccaatt cagaaacagg ggagattact tgcactaaag aaagtctaaa 42660ggaaggtagg agacaaaaat aaagttgtgt attgatgatc ctaggagtta tgcttgtttg 42720acataccagt tatacctgct gtcacggtag ttatgcatta ggggacccag gtgtctgaag 42780ttatatccag aagacttctg agggtgcacc ggggggtccc ttggctaaaa gtgtgattta 42840aaccctaaga gcctgcccag actatcagtc ccagtttcta cgtccactgt ccctgaatct 42900cgctgcttct tccttaggct gctgggagtc tgaaccctcc cccgccaaca cccctccccc 42960atgcctcagt cgtgggaagg gggggccctt gagcagtagg gccaagccct gttcagcctg 43020ggaccaagtt cccatcaaca aggtggtctg ggcagtggcc agccagaaag cagtaattac 43080tgtcgaggtg cagggacccc aggtagggcc cccacctccc acctctgtgt gggcagtgaa 43140tgggcctgcc cctgggtaag gctgtgtcag caggcgcctg cccacccctt gctgggttcc 43200caggccccta gagccctctc gtaataggag ccatttgcgc tgtaaccagt gggtgaccag 43260atttttaatc ttggagaccc cttggatccc aggcgggaag tgggatttgt caaatgggga 43320gaggcggggc tgtctgggaa tgccagacgg ggttgtgctg gggaaatatg tctcctttcc 43380atacagcccc cttcccatac ctccagcctc cctctacccc gcaagtcagc tctgtagctc 43440ctaggaggta tctccaacat gcttagctgt tgaaagtaaa tgaatgccgg aagttgaaat 43500ctgaatggct tgttttgcac taactcaggt gcatgccaaa taggggtgtc tctttgcttg 43560atcctaatcc ttcctcctct gaaatccttt ctgacctgct gcctatcagc aattgccctg 43620caaagaccct ctagctggcc gcgggagaag ctgtgttctt ggctatcagg agtgagaaca 43680ctggacccaa gcttgcctat tctctgccac aactcactgt gtgatcttgg acaagtcact 43740tcctttcttt cagcctcagt ttcaggagac ttggcacttg ctgagtgcca atgtgtgcca 43800tgttcttgac aagtatggtc ccacacaacc ttgccaatga ccctgtgacc tgtgtcttct 43860tgttcccagt ttacaggaga gaagatgcag cctgggagag atgcagcagg tgtctgaggc 43920cacacagcaa gtcacccagg gccaggatct gaagctgggt ctctccagct ccactgcctg 43980ggcactttct cctccacagc gaccttcagg tcatcatgag gagcctttcg gactaaagct 44040agagagctgg gattccaaca gttcagcaac ccatgacttc tccatggcag ctgctgcctg 44100accacctagt gcctttccac taagattgtt ccttccctct cctgaagatt atcctcctgc 44160ccctctctcc caaacatctg cggtgtgcac ctgctgccca aagttgaact tttttttgtt 44220agagacaggg cctcactgtg tcatccaggc tggagtgcag tggcgtgatc atagctcact 44280gcagcctcca actcctgggc tcaagtgacc ctctcacctc agcttcctga gtagctggga 44340ctacaggcat gcaccaccac gcctggctaa tttttaaatt ttttgcagag acagggtcta 44400gctatgttgc ccaccctggt cttgaactcc tgggctcaag caatctgtct gccttggtct 44460cccagctcgc tgggattaca ggcatgaatc accatgcccg gtcctccaaa gttgaacttt 44520tgagactcag tttccttctt ggtaaattca ggctcctagg tgctacctcc tcagagacat 44580cctccttgac cacatcacat gacctctcgc agaggccaga ttctttaatg tattcatttg 44640tctattatca tgtttcccca gtagaagttg cacaaacggc aggggcttca tctgttttgt 44700ttgccgttat gtccttagca cctaaaattg tgcctggcac atatagtact cagtatgtat 44760ttgctgggtc aatgagtgag tgaatttata ctaataacag cagctaccat ttctagagtg 44820tttaccatat attgggcact gtgtcagtct tcccaacaac ccacagacga agatcaatta 44880ttacacccat tgtacagatc aggaaactga gtcaggttaa gaaacttgcc ctaaatccta 44940cagtctcact tagaacttct gactgcagtg ctcatcagaa tgcattgtca acccaaaggt 45000catttccagc tcaggtggct tctatcaaaa gagctcatcc tggcctttcc aagagccaga 45060cctccgacat cggtggagcc ctgtgcatag ctggcctctc ctgggcgtct tgtcccaagt

45120acagagacct ggatcctttc ccactcatgt gcaacagccc aaaattaaaa acaaaagcca 45180tattaaaaaa caaaaccaac tttctgcctt aaaatattgt gagccagggg gcaattagca 45240attatgctgt attttattat gagaagatag aattctaatt ggactgattt gaattccaca 45300cacctccaca gattgttttg ggaattaagg tatcagttgt atcggtaatt atggtttacc 45360attcaattac ccccccacag aaaactgtta aattgtctgt gacggggctt aaatttagct 45420cagacctatg tcctatgaag actgcgcgag tcaatacaag ccatccggaa accaccgggt 45480gccctgtgcc aggcggtaat taggggttga ggtttccaaa gttttacctg agacagcagg 45540gacaagtgcc tgggctgggc gtgctcacgt gggggggctt ggatgctccc ccagcacagt 45600gctcttggct cctgccctgc gttgctggtg caatagctga tcatctggaa gacaatgtgg 45660tttcagccgc aagtgacatt ttggcgaggt gcaccagcat agaagccctg agacagcgag 45720ggaccatgta aaactcacgg acattgtaat tggacacatc tgggattgaa ttcccactct 45780tccacttagg gagtgaactt gagagggcca cttaatatct ttgagcctct gtttccccac 45840ctgtcaaatg ggtacacctc ctgcctcaca cacgggttat tgtgaagatg gatggagaat 45900aataatggtg cctggcccaa attgcatttt tttctctttt tttagtactc actacatgcc 45960agatacttta caaacatctc taaacctcct aagccctata gggcaggcac tgtggttatt 46020ctctgtttta tggatgggaa aactgagtcc cagagaggtt aaataacatg ccgaaagtcc 46080catgactatg aaatggggca gctgggatcc aaacccaggt gacctagggc caaaccctaa 46140gcatgaagct gccctgctgg gtgccttctg tacctgtccc tctaagtgga agtgcctaga 46200aatgacccag ccaaaagcag cagactgtat tttatcattt caacaattct ctctgtccat 46260aaaaagttct atgcagcttg gccagttcct ttttcctgaa tacaaaactg agaatggtaa 46320cagggaactg actttttaat gtcatggtgc aaaggagcta gctctgccat gacctccttg 46380aagtgacctg gtagagtgag ggtaagacaa gccacactcc ctaggctatg gaggccactc 46440tccatggaga taggggaata gggaatcctg caaaatacag tctctgggga tgggaaggat 46500cagggaaggg gccaggtgca gtagctcatg cctgtaatct cagcatttcg ggaggctgag 46560gtgggaggat cgcttgagcc caggagtttg agaccagcag tttgagacca gcctgagcaa 46620catatcaaga ccctgtcttt acaaaaaatt aacaaaaagg ccaggcgtga tggctcacac 46680ctgtaacccc agtactttga gaggccgagg tgggcggatc acctgaggtc gggagtttga 46740gaccagcctg gccaacgtgg tgaaactccg tctctactaa aaatacaaaa attagccagg 46800tgtggtggct gtaataccag ctactcggga ggctgaggca ggagaatcac ttgaatccag 46860gaggcagagg ttgcagtgag cccagatcat gccactgcac tccagcctgg gagacagagt 46920gagactccgt ctcaaaaaaa aaaaaaaaca attaacaaat tagatgggtg tggtggtgca 46980cagctgtagt cccagctact caggaggctg aggtgaaggg atcacttgat ccaggtgttc 47040gaggctatag taagctgtga tcaccccact gcactccagc ctgggtgaca gagtgagatc 47100ttgtctcaaa aaaaaaaaat tttttttttt aaaggatcag ggtaggtaca tggactcctc 47160ctattccttc tggcaccatc agtggttgaa cacacaaact ttggttcaaa tcctgcctct 47220tccaatttgc tagctgtgtg accttgaaaa agttacttaa cccctcttat ccttagtttc 47280ctcatcttca gaataaaaat agtgcttgcc tgatggggga gatgatggat gtaaagggcc 47340tcatagaggg cttggcattt accaagtgtt tgagaaatat tggctgtttt tattcaaggc 47400ctgctcattg ctagcatgag ctacctgcaa cagccacctc taggcctctt ttgctcccta 47460tagtccagcc acaccaagaa accatgatgg cctgaatcct ctctgacttt gtcaggcctc 47520cgtgccttta tacatgctgt tccttctgcc tagaaggcct ttctcttttt tcaacctcct 47580tgagtccatt ttagtccctg ccagatggtc acttctctgt gatgctgccc taactccttg 47640gcagccatgc tgctcccttg accattcact agccccttta attcagcccc agccacagtg 47700aggattaaat gggatgatgt ctgagaaagc ccatgggcaa gaacctggca tggagtggtg 47760ccccataaat gatgaatgga gctggggttg ctttctcagc cagctgcctg gcaggggcct 47820ggcttcctca tcttcacacg tcagtgccta gagtaacacc tggcacacag tgaacacaat 47880ctcagtaact gttagacatt tgtcttttgg ggagctgctc agcctcccac attggggtgt 47940gggttttttg ttttgttttg ttttgttttt tgagacggag tttcattctt gttgcccagg 48000ctggagtgca atggtgcgat cttggctcac tgcagcctcc gctgcctggg ttcaagtgat 48060tctcctacct cagcctcccg agtagtagct gggattacag gcacgtgcca ccatgcctgg 48120ctaatgtttt gtatttttag tagagacagg atttcaccat gttggtcagg ctggtcttga 48180actcctgacc tcaggtgatc cacccgcctt ggcctcccaa agtgctgaga ttataggcgt 48240gagccaccac gcccggccta ggggtgtggg gtcttttacc gactaggcct ggtgggggaa 48300gtcagggacc ccaccacaaa agttggagag gccacatcct cccacctcca tctggggtta 48360gacatgtgac ccaggcttgg ccaatcggat gttcctccat cttgcccaga attcacactc 48420agcagtggtc ttcacgaggg gtggtagtgg tggccagtag gagcagggct gggcagcctc 48480cctaacccac gttcatgaga cttgaagctg gctggtttcc tgtcccctgg cctcccttgc 48540ccattcccat cctggtgctc cctccctatg gactcctttt gctcataaat atccctccaa 48600ttaattgctt tgctgcttct tagctagtgt tgtttctgtc acttgcaacc aagagcctat 48660tcaggtgcag gaagggttga atgaacaatg gaaatggatc aacacatgtt tctgagcatg 48720tgctgacagg caggccctgg gcggagtacc acggtgttca aatgatgtgt aagatgtggg 48780cagtgccccg gggggtctat ggggagacag accatgcatg acatcataaa caagaccaca 48840gggtttagtt ggaccttgca gacacccaga gaagacgact ttgagccctc attggatggt 48900gccatcgcaa agtacaaaac gaattgggtt gtaggaactg gaggcactgg gacaatattc 48960cagattaggg attgagctga aggggttgga aatggctgag tagggtggtg gaaagggatg 49020cctgctagta ggcaattgtc ttagtctgtt ttgtgctgct gtaatggatt accacagact 49080gggtaattta taacaaacag aaatttattt ggctcatggt tctgaaggat gggaaatcca 49140aaatcaagga gccggcatct ggtgagggcc ttcttgttgc atcataacat gccagagggt 49200gtcacatggt ggaagggcaa agagagggag aaagggaaag agagggagca ggaaagggca 49260aacccactcc catgataata agctcactca tatcatgagt cattcatcca tcgtgagggc 49320agggtccacc tcccaatacc atcacaatgg caactaaacc tcaacatgat ttttggagag 49380gacaaacatt caaaccatgg cagcagcgtt tgtgggaaac acgcgccaaa gcaagaaaag 49440accaacctaa gagtgagtga cttggctcct cattctacat tcattttcat ccaatggggc 49500ccaagggcat gtacccatta cccatctggg cagttccctt gaatgtgggc ttctgtttgc 49560ccgtggaggt gaggaacttc aaggaagaaa ccatgaaaga cctcttgagg ctgagggctg 49620gcaccagcac caagatctcc aggcagctgg aacagtgatg gctcctccgt cctcgcaggc 49680ggggcaccca acagggtgtg accgtcacct gaggggagac agccagaggc acaggcctga 49740tcctgggact gaggttggcg gtttgggtgg agaggtgatt ctgagtgtga caccctccag 49800tgataaagtg gggggcttcc cagcagcccc tggggaaaca ggctgcatct ctggagacag 49860gagatatgtg gaggcctgag gggcagtgga aagccctgtg tgtctgggtc aggtctctcc 49920ttggcaggta atggtttgtc ccagtagttt tcagactccc catcctgccc tgtccttctc 49980ctgtctgatg ctcagacctg tcactcccag ctcagccccc accttactgt ctacccctca 50040gagtccctcc caccaagggc accttctgtc ccacatctcc atggtgcagt catgggagga 50100gaacttggga gcataagaaa ctccaccaag ttggggccag gcatggtggc tcacgcctgt 50160aatcccagca ctttgggagg ccgaggctgg tgaatcacaa ggtcaggagt ttgagaccag 50220cctgaccaac ataatgaaat cccgtctcta ttaaaaatgc aaaaaattag ccgggcatgg 50280tggcaggtgc ctgtaatccc agctactcac gaggctgagg caggagaatc acttgaaccg 50340ggaggcagag gatgcagtga gccaagatcg cgccattgca ctccagcctg ggtggcagtg 50400aaagacttgg tctcaaaaaa aaaaaaaaag aaagaaagaa aaaaaaagaa actccaccaa 50460gggcttttgg gtcctaggga cagcaatgat gtggctggtc cagccagaac ctcattttgt 50520cagtaaagtt gggacccata ttccctaaga gatttgtccc aagaacacat gggttgctac 50580agcggaggag aaatcaagtc tgttttccct ctggtgaatt gtcccctgaa cgtgctttct 50640tcctacagtg ttgccaggaa agtaaaaaaa aaaaaaaaaa aaaaaaaaaa gtccacgatg 50700tcagctgggg tgataccaaa acaattgtgg gaggaacaac atccgcaaat tgaatagtgt 50760gaggagtgtg gacagaagat gttttgtctt tggcctcatc tcccagactt gatctttgta 50820aatacagaag tttccaccag agccgaacct ggcaatgact tgaggagcag ctgcaaggaa 50880gacagcctct cccagggtat cacctggggg cacaccccag cttcccctcc tgagcctcat 50940cgaggggtta gtgctacctc tcgggaaaac ataaagatga caagaagcca aaggtgccaa 51000tagttcccat ttagtataaa agctggctca gcaaatcatg ctatttcagg gcctagggtg 51060ggccagttcc caggcagccc tggcaggaag gactctgaga ggcggacagt aaggtagggg 51120cttgggagtg ataggtctaa acatctgatg gaagcaaaag ggaggacaag ggagggagtc 51180aaaaaatccg ggaagcctgt ccttccagaa agtaccgaca ccagggtgag gtggatccca 51240gcacccgcac ctctgaatgg cctttcccct gcacccctat cccgccccca ctcggagcta 51300ccccaagcac ctgttcctct gggccccaag gtgacgccct ttgtgtgtgt ataggaaagg 51360acggtgtaca catttggtta gttccttctt ttacaccata aattatcaga gacaaccctt 51420ttggaaagca atttggcaat aatatcaagt gacataaaga tgttcatagc ctttggccca 51480ataatcttcc tcctgggaat taatcctaag aaaataattc acaaggaaga aagaaccatt 51540ttgtatctac aaagacattt attggggtgt tatttatgat agggaaaaac tggagacacc 51600ctccccagcc aacatagagg gatggtccta taaattatga tacatccatc caatgaaatg 51660ttaccctgcc agtaaaaatg gtaaattgaa aattgtgtag caacaagaaa gagtgttcag 51720aaaataaaag caagtgaaaa aaggagcaca ggattgtcta tatgctgtga tcaatggcaa 51780tgctgacaaa tccatgtatg tgtagatagt tgtagtgtta cctgtaaggc acgataaggc 51840ggatgtaact gtgcttcaaa tgttcttttc catagctgtg gagtccttct agtatgaaat 51900catttttgta aaatcgcaat ttgtcaccag gggttttgac tttccttatt gcctcgtggg 51960aggtggcaga ggcagcacat ctcagaccga gcctcggttc ctccccctac ctcccaccgc 52020tgcgcttgaa gaaagatgct gcagcctccc cagtccccat gccagcgctc ccacttttct 52080ctgagctttc ggtggcagac agcgccttgg gcactttttc atgctcataa ttcgaattac 52140ctgtttaagt cggtcaaatg aaaaaatacc agctccgccc ccacgcgggc tggccggggc 52200gcctggagcg ccagggcggc tgcagcgcgc tctccgcggc cgtcggccct gagctcattt 52260cctggggcgc gcgcgccggg ctatttcagc ctggcgctgt gcaaacagga caatttactg 52320cggccaaaag ggacccaaat tacaatcgta tcacagacaa atatccgcca cgccaggtct 52380ccaggggcca ggaggggcct ctctcccggc gcggggggcg ggcgcggggt caggcaggtc 52440cgcggggctc ggctcggcct cgccgtgccc tgatcggcgt ttgccaccga gctgtgcctg 52500ctctctgcaa caggaagggg cccagctccc ccgggcgacc gttcctatct gagttctcgt 52560tcctatctgg gttttcgtgc agaaaaactt catctcttcc cagggatttt cccctgattt 52620agggtcccct tttatttggt ttcttttcag cacttgggat gaaaacatcc ctccatccag 52680ccactccagg gctcaaagtc atatctcctc gttcagaaac ctcagtgcct gtccaccact 52740cttccggaaa agtcggaatc cttagtgtga tggtcaaaga ccgctcttag tccagtccac 52800ctgtccaggg ccagcccctg tacccagatg ctccaaccac ggagagcaca tcagactttc 52860acaattttat ccttgacaat tgccttttca tcctgcctaa atgttccctt gtttctttct 52920gcctagtgag ctcttattcc ttctttaaga cccagataca catcacctct tccatgaagc 52980cttcccagac ttcccctttc cctgcttgac atctgccttc caagcagagt cagccccttt 53040tccttcctag caccgtgcag aggtagatgg tacttctcac atcctgtaat taattgcttg 53100catgcctgtg gtacaccgta ccattgctca caaatatttc ctcttcctct ctggagaagg 53160attacacttc cttgatccat tggcctcact caggcttcgc cacatgactg gtgctggcca 53220gtgaagtggg acatgtgagt tactggggag cagaaacttt caaagccaag catggctcac 53280cttgttctct cttccctctg ttacaaggat ggcagtgccc cagccagaaa ccacactgcc 53340agcctggcat aggagtggag cagggcacac ataagtgtga gagaaatgaa cttcacctca 53400gtgctcgagg cccctgggac catggactca tttgttactg cagcataaac tagctggtct 53460tgacttcagc ttctgcctct cttccctggc tttgccttca caaaatcaag agtctgatct 53520tactcatatt atagagctgg tcttcaacca tgttcttttt ttttttttcc tttttttttt 53580tttgagatgg agtttcgctc atgtagtcca ggatggagtg caatggtgcg atctgggctc 53640actgcaatct ctgcctccct ggttcaagcg attctcctgc ctcagcctcc caaatggctg 53700ggattacagg tgcttgccac cacgcctagc taatttttgt atttttagaa gagataggat 53760ttcaccatgt tggccaggct atctcaaact cctgacctca ggagatctgc ccgcttcggc 53820ctcccaaagt gctggcatta caggcatgag ccaccatgcc cagccccatg ttcttcgaat 53880ggccaagaat atcagtaggt aaggtttgtt gagcatggat tgtgtgccag gtgctgtgct 53940atgcctttgg caagcgttat cccattacat cctttaacag cactctctgg tgggtactgc 54000tcttatacaa gatttacaaa agagaaaaga gactcagagg aatgtagggg ctcacgtgag 54060attgtaaagt agtgagaagt ggagctggtc cttcagctca aatccctctg atgccaaaga 54120actcaaactc tgcagttcca tcatgtgcat cctcactctc tcacttcctg cagaggatct 54180gtggaggttg taactattgt tttcacctgt ctcttccact gggctctgag ctgcccgaag 54240acagatcttt tttttttttt tgagacagaa ttttgctctt gttgcccagg ctggagtgca 54300atggcacgat cttggctcac cacagcctct gcctcttggg ttcaagcgat tctcctgcct 54360cagcctcccg agtaactggg attacaggca tgcgccacca tgcctggcta cttttgtatt 54420tttagtagag acggggtttc tctgtgttgg tcaggctggt cttgaactcc cgaccttagg 54480tgatctgtct gtctcggcct cccaaagtgc tgggattata ggcgtgagcc accacgcccg 54540gccccgaaga cagatcttaa tcatctgatt tcccatccat taattcagtc tttcaccaag 54600gatgtattga gtgccaatta tatgccaggc atcatctgat gtgttgggga cagagaaata 54660aaacagataa aaatccctgc tgttaattct agcctatggt gacaagaagc agatccgtgg 54720ctgcctgggg tgagtgtggg gtaggaaatg ggagaaatgg ggatgactgc aactttgggg 54780gtagtggaaa atgttcagta tcgtggtagt ggtggtggct gtacaggtat atatatccgc 54840atacatccca cagttcagaa ttatacactt taaatggtaa atggatacag tttattatat 54900gtaaatgatc cctcaattaa gttgatttag aggaacctgg ccgggcgtgg tggctcacac 54960ctagtattct cagcactttg ggaggccgag gtgggcggat catgaggtca ggagatagag 55020accatcctag ctaacatggt gaaaacccat ctctactaaa aacacgaaaa caaaaaatta 55080gccgggtgtg gtggtgggcg cctgtagtcc cagctactcg ggacactgaa cctgggaggc 55140ggagctggca gtgagctgag atcgcaccac ttcactccag cctgggcaac agagcaagac 55200tctgccttga aaaaaaaaaa aaaaaaagaa cccttgctct tatggagaag cattctactg 55260gggaaaacag acaataaaca catagataaa taaaacatat gtcagaaagg ggtaagtgat 55320gctcagaaaa ataggacaag ggatcagaag tgctaggcgt tgcaatttaa aataatgggg 55380tctggaaagc caccctgaga agggccaatg gagctatgcc cagcctgtgg tatccttcct 55440caggcagggg actgtacccc cagcttacaa gccttttcga ggcaccttca gggtttgagc 55500ccaaaatgca ctgcacatta agtgttattg atgggcttgt ggaaatctgc tctccgtcag 55560gctctgatga aacttttcca acaggagact gaactcagta ctgggcatgg ccccctgcac 55620atagtaagtc cacagcaaat gtgtgtgggg tgaatactgt cttctttatc ctcccttttt 55680ctactccagt ccaggtagac ggtgtatcta cccacccaat tttgctgttt ctggtaccct 55740cagtgggtct tgcctgctct tcctccttga aatcattact cagaggtctc cgtgtcagct 55800ccagacagat gggcctggtg ctcctttatc tcaatacaat tcccccgctc ccaggctcca 55860ggggaaatcc tgagctaact ttggtctttc ctaaactcca cacttccctt tgggcacctc 55920tttccccatt gcggggctcc ctttcagaat ttgctgcttt atttatttat ttatttttgt 55980cagaggtgca ttaatgatgc tttatttaaa aacaaaaaac ttggccaggc gcggtggctt 56040atgcctgtaa tcccagcact ttgggaggct gaggtgggcg gatcacaagg tcaggagatc 56100aagaccatcc agaccaacat ggtgaaaccc agtctgtact aaaaatacaa aaaaaaaaaa 56160aaaaaaaaat cagccgggcg tggcacatgc ctgtaattcc agctactcag gaggctgagg 56220caggagaatc acttgaaccc gggaggcaga ggttgcagtg agccgagatt gggccactgc 56280actccagcct gggtgacaga gcgagactcc atctcaaaca aaaaaacacc acaaataaat 56340aaaaaataaa aacaaaaaac caaaaacagt ccattccatg tcgtgttgaa actgatcagt 56400gtaagttaaa tggtggtttt taggctggac ccatgattta agctgtaccc atccagctca 56460aactgaaaaa aaaaaaaatc atttgaatgt taaagcaatc gttcagagtc ttcaagaaga 56520aaccaggcag gaaaatgcca ataatgatga ctggcaaaat caaaatctaa aacaaataaa 56580ctgtttatca agctgccgac agaaaaagaa atcttgcatg gagactacaa gtctggattt 56640tctgggatga aattgtacag gaatctcagt ctacagtttc ctcaatcgct gtggagatgg 56700agctgtcact gaatctgaca gagccctgca ctccccagtc cgccgaccct ttctgtaatc 56760cagtcttcac tgtagcctga ggaactattt caacctgctc cttttttatc ttcttctttg 56820gcacaacctc agtggacttc tctgattcag aacaagttct aattgatctt ctctgttgct 56880tcttttctac tgagcctgta gaaccagatg ttgcttcaag agatgatata ttctgcattg 56940gcttttcatt tctctggttt ggtttagaaa ttataagcct gtcttgcccc ctgacactta 57000tttctgtttt gttaccaatt ccctttgttg aataaacaaa ttaatttccc atcctctgta 57060gcattctgaa gagcaaacac ttcttcaatt ttcacagctg gagacatgtt acacttctgc 57120aaatccaggc tccctttgtg cattgtaatg gaagctggta ggatttcctt gctgccacag 57180ttttccaggc tattttaaca ggaggtggct cttcctcgtc cgcgcttgtg tgctgcctcg 57240ggctgtgtct ccaaatgtca gtacttgaga gtgaggaggc cacctcccct gcattgatct 57300gttctggctg agtttaaagc acagatcttg gtcatcaggt ttttttaact tcggctttgg 57360agacaacatt cttttttttt ttttttttag atggagtctc gctctgtcgc ccaggctgga 57420atgcagtggt gcgatctccg gttcatgcca ttctcctgcc tcagcctccc gagtacctgg 57480gattacaggc gcccaccacc atgcccggct aatttttttg tattttttta gtagagacgg 57540ggtttcaccg tgttagccag gatggtctca atctcctgac cttgtgatcc gcccgcctgg 57600gcctcccaaa gtgctgggat tacagacgtg agccaccgcg cccggcccca acattctttt 57660ttgcttggga taaaccctct tcaggctgtt aatcaatata gataaaagta tactgttcta 57720ttctttcttc tcaagtcatt ttcaatgctt tctctgcatg ggcaatgcca aaatcccatt 57780gagcatgttc tctctgaggt cagggtttcc aaatcttttg tttctcagag tgattgctgg 57840cttgtttggt tgcctcagcc agtaattctt cataccgctt atgaccttta tactcctgta 57900cccatttttc atgaacccac accctctctg gctgtttgct aaaacactgg acatgatatt 57960ttcgggcacc tcctgtgtta attttggtat gaacctccag ctggggatca cttcaatcca 58020tacaaggcca gcacggatag gttcccacct tggaccacac aagatcacca acttgaaact 58080taacaccagt ggacacttct gttgttggaa cagaagatag tattggctga actggggctt 58140cctcttttag tactggatct tcccttggtt tttctgatat ggtgtgaacc ctctcatttg 58200gtctattttg ttcctctggt tctaatttgg ggattttgtg tgacttgcgc tcttcagatc 58260ttgatgagtc atgctttttg ggttttttcc tcttttcttt tctgctttca tgctttgagt 58320tcgtgtgctc acttgcctgt acttcattta aaaggtctcc acaaagggaa gacttgaaca 58380attccctgcc attctggata gttttggtta tttttagttt aacttcaggt gagccactct 58440tctttggaat cacagtttgt ggtaccgaag tggtggctgt ggaggggaag ggttttctag 58500aatttaatgt ggtcttctgt ttagaatttc tgaatggtaa tagtcagtgg ggctaaagtt 58560tctagctgcg ccaaagtcat tggctgaccc attaggatat tgattatgcg actggtattt 58620ggtttgaatt tcatacatgc tgattgatgg caggtagcca tttgtgagtg gaggaagatc 58680tgttgtaggt gagtattgaa agctttgctg caaggtagct tcgtatggtg tctggccacc 58740atcttcagca atgtcacttt tgttatcaaa ggcatcctcc tgacggatgc tggcggagtc 58800agtgagttga ggtggttgtt gaactgtgtt tcccgtgatc ccttgcatga aagagaaaga 58860gaaatccatt gttctgctcc agcattctta actttccctt tctctcatcg ggccttcagt 58920ttgtcccgtt ctaaccgccc ggccgtctca atgcgatcac aggagggaca cggagatcga 58980ggagggccac agccggacca ggccaggcgt gctgcggcag gaggaaggct ccggaggtgg 59040ggtgggaagg ggaggccgag agacgggtgt cgccgcgccc ccgctgccgc cagagaggag 59100cctacggctg gcagcctggc ctgggcagca gggtcctcgg cgctcggctg ggaaatcgca 59160cgtctctccg cggtgacctg tgcacagccc ctgggcctcc gcctccgtgc tggcagcctc 59220cgcctcagcg cacaaagccc cgtcaccccg actctcggag cgccgccgcc gccaaatcct 59280cagcccctcc ctcattggcc gcggcgtctg ccgggaagtg cagtcccggg tttggggcga 59340tggagcccag gaggaagcgg cgagtcagcg cggcggagag ggcggagggg acggaggggg 59400cggaagggga cgaaccacga acgcccgcgg ccgcgaaggg tctggacgac aaaggagaga 59460ctagcgagag ggcttgttgc tttttttttt tttttttttt tttttttttt tgagacggag 59520tctcgctctg tcacccaggc tggagtgcaa tggcgcgatc tcggctcgct gcaacctccg 59580cctcccgggt tcaagcgatt ctcctgcctc agcctccgag gagctgggat tacgggggcg 59640cgcctccacg cccagctaat tctttgtact tttagtagag atgaggtttc accatgttgg 59700ccaggctggt atcgaactcc tgacctcgcg atccacccgc cttggtgttg cacttttata 59760actcttttta ccttctcacc aaactagcat ttaggaaaaa aaaaaaaatc aacaaaaaca 59820aaacaaaaaa accaaaaacc tctatcagtc atttacacgt tagttggaaa taatttagac 59880cagtgtgtct ctccttcgca gaacacattc agtcctctct gattattaga agcaaggttc 59940accttttgat cttggttaaa gaaatttcag gctaaatttc tggggaggag atgtatttca 60000ggttccagga tcccagaatt gctctgcaca agtcacagcc atccttgttc cctgaagtct 60060ctgttggctg caccatgtct gctgctcctt tatgagtggt tttgggagag gccaggctga 60120gctcttacta gctccttgca acaaaacaag cgaatgtgaa aagtcttaaa cactcagctt

60180tctggtcaga ggaaatccca aaatcagcat gactcaggat tttaagtgcc ccatagagtg 60240tccaagagaa gaaacctagg agtctagggt ctttcaaaac cccaaagtta aaacctttta 60300tcaccctttc ctgaccttcc tggcttagga ttttaggcag cctcttggag gcctcccttg 60360cctggggcac acttggccat aaacccattc attcagccct actgatttag tgcccattat 60420gtgccaggca gaggaaaagt agaaatggta ggatctatct tcaaggggct caaagtctgc 60480aggaaacagg atccgctaac aaattagagc acaagggtgt gccgagtgct ataatagcag 60540tgtgggttgc tgttgagaaa atacagagga gggtaccacc agggagcttc tatgagttga 60600gctgagcctt gaaggttgaa gaggcatttc tcacatagag aagacgaaat agtaccctga 60660ttgtatacta ggaatgaaaa caccaagtgc ttaccaagct cagataggta acgtaaatga 60720atggagtagg tgagtaaagt agcagggatt gtcagcggtg gtggggaagt ggaaggcctg 60780catcctgcct acagggcttc tgctggaaag acagcctggc ctggattcag cctccatttg 60840caccttctgc taggtgcttt tgtgcagtgc acaactcaca caacctcaca tggctgccct 60900gcctgcctaa gctggccatc agctgcttgt tgccatactg aaaaagaggg tttggttttg 60960cctcgccttg ctttttcaat agaagccaga aacttgaatt tttatgtaaa aatttccaat 61020ttgtcaaatg ccattgtagc caaacaaaaa tctagcctca ggctggatcc accatagagt 61080ttcagtttga gaccccgatg ggaaccagtc ccctgggcac atgctgctct ctctcctcca 61140taccatgcaa cacagtcctt tgctgagatt tgacaaggga aacacagtca tagatgaaac 61200cccctacagc tgctgcaaag cccttcagaa acatgagatt ggcctctgaa ccagaggaaa 61260gtaacgtggg gctgtacaac agcagccagc ctgtgagact gatggataaa gctttaattt 61320atcggtgcac cttgtttgga gaaagcttcc ctgccctctt ggctgccccc acctccaacc 61380cctcctgttt tgctaatgat aaatgacaag cagtggacat ttattaatta aaccacccgc 61440tgtagccatt ggtaaaaccc aaggccacat gcaggggaag cattcaaagc attcctgctg 61500cccctgccca gggcacaggc cccagggtta tcctttgctg gagagagcag gccacggcgg 61560ccacagcctc acatgatggg ccttcctagg atctgtctgg gttctggctg ataaatggaa 61620atcacctcca tgggccacac agtaattaaa ctcctggcat tcttttgaca aaaaaaaagt 61680tcctcatggg acattcccac aagctgctga aggtctggga cctgcaagct cccagctgat 61740taccaaggag ttttcgaagt tggccttgac tgaggatcaa aggaggatgg gagttcaggg 61800aatgagggtg ggggtgggaa atgccttaga attaagttga tgatggcctg ttgcggtggc 61860ttatgcctct aatctcagtg ctttgggagg ctgaggcggg aaaatcactt gagcccagga 61920gtttgagacc agcatggaca acatggtgag accttttctt aacaaaaaat ttaaaaatta 61980gcctggtgtg gtggcacatg cctgtggtcc catctacacg aggaggctga ggtgctagga 62040ttgcttgagt ccaggaggtt gaggatgcag tgagctgtgt ttgcaccact gcacttcagc 62100ctggtgaagg aggagaccct gagacagaag gagaccctgt ctcaaaaaaa agttgatcat 62160ttggatgttc ggcctgaaac acccacattt atttccccag gaccgagatg ggcagagaga 62220taaaagcagg ccttcaaggc tcatttattt atatttctac tgtttatttg ttccactgat 62280gtaccccaag tacctaaaac agagcctggc acatagtagg tgctcaataa atatttgttg 62340actgattagc tggagatgtg ctcatgtctt tttaacgaaa gcagtaatca ggagaaattc 62400ctaattaagg tcctagaacc agtggcttct aattgtttgc tgccacagga ttgtaaacat 62460gttgaagatg aggccagtgt cttattcaat tgactgtccc cagaagcagg cacagtgccc 62520agcctagggc ctacacaccg tggtttctca acgatatcaa ttgtctgaat gactcagggg 62580taaaccattt ttcagtgctg ggtcaggcag agccccactg ggacgtgacc tgcacacact 62640ttggcagtcc cctgggatgc agtggtagca gcagcagagt cataaagcct ggggatcaga 62700gccacactgg atatcacagc tggaaaggcc tgagatctca gccagtccct tgctaagcag 62760aaagaagaaa tgaattagct acaaatcttg gggcctctaa aaacacagct tcatttttct 62820gtttctccta tagactcccc attctgtcca actgccaaaa acatttaagt ttatttaata 62880atcaatgtca gctgaagttg gtcatatcag acttaaattc aatctattcc cagttgcctc 62940aacttccctt gtcaggtcat ctacttagtt ctagtggctt ctgtgccata ggagggggag 63000ctggaggagg gggagagccg agccatggtc atcagtatct gtcttcacta acctcttggg 63060catggtcacc ccattttgtt ggtgtctgct aaatccacat cttttttttc tgtagtaatt 63120agcccacaca ctctgtgtcc tcctgactct gcagctgagg ccctcccagc ccaccaggcc 63180ctgcccagca ccttccatgc cttccttgga agtgttggaa aactagctgg cccctcatac 63240tatagggacc cagggaagtt ctgattgacc ctgggtgcca tgatagaaca ggactccacc 63300cactcagcat tccagataaa cagtggacac agtctccttt gcactcctat tttcccaact 63360ggttgggagg attctgttgg tgcctgctcc ccaccctatt gggatctctg tctccctggt 63420gagctgtgta tgtgcatgat ggtgagggtc tccagcaaca aggaaaggag ttacatttgt 63480ccatattcac actgctataa agaaatacct gagactgggt aatttataaa gaaaagaggt 63540tcaattgact catagttcca catggctggg gaagcctcag gaaacttaca atcatggcgg 63600aagggaaaag gcatgtctta catggtggca ggtgagagag agtgtgtagg aagtgaaggg 63660ggaagagcat cttataaaac cgtcagatct cctgagaact cactcactat caccagaaca 63720gcatggggga accaccctca tgatccagtc acctcccagc agctctctcc ctcaacacct 63780ggaaattaca attcaagatg agacttgggt ggggacacaa agcctgacca tatcagaggt 63840tgatcttgca gcattttatt ctctgaatgt cacatgggga aacggaggtc aagaaggtgg 63900aaaagacttg cctaaggcca cacagcatga gtgagtgtgc ctaaggccac acagcattgg 63960gccaagagcc tgggacttgg taagcccctc ctgagccctc ctcctgagcc aggccctgtc 64020tcaggttcta gcacatgcaa ctgcaaccag cagggtatgt gtcatagtct taaaatatgg 64080ccacaaattc tttgatggtc ttcccattga gctgtggggt ctatttctct cttttctttg 64140aatattggca gggttatgac tgcttcaatt aattgagtac agagaggtga tgctgtatga 64200cttccaagcc taagtcatct caggccatca gcttctgcct ttgttaatgg aacacttgcc 64260tttggagtcc tgagcctcca ggtaaaaagt tcaactatcc caaggctacc atgctgtgag 64320gaagcccaaa ctacacagag gggccatgtg aaggcactct ggttgacagt ccccactgag 64380atgtctcagt ccaggcacca ggcatgtgag ggaagaagcc cccaggtgat ttcagccctc 64440agcctttcca gccacctcta gccatttgag tcttcacaac cctggcccta gacattggag 64500agcagagaca ggccaccctg ttgggccctg gccaaattct taaacctctg aatctgtgag 64560cctaataaaa tggttgttgt tttatgtcac caactttggg gtggcttgtt acacagccac 64620agatagctgt gccatcttaa cctgctggat tggtacccct taagtctctg atatccatgc 64680ttctgcccgg ccactaatga agccacatct gttcccacca gaagaccact gagaaggcca 64740gtgagtgtgt ggtggggacc cttgtttgaa ggaagttggg tgaaggaagt tgctgacatg 64800catgactgct aacaaaagaa tatacatctg cggaggttgt tctggaagta tcttagggaa 64860gtaaaggaaa ggaattaaga ctcatcgaca acagactcca ttccttgccc tgcccatcct 64920tattcattta ttcaacaagg ggtctatgag caactacgag gtgccaggcc ctgtgttaag 64980attctgggac atggacacag gcctggccct agtggttctc ctagtccagc tagagacaaa 65040cattaaacga tgattaatat acatactttg aatgaaaaac ttagggtgtt ctgagagtgc 65100gtggagacct atttaatcct cataatatcc ttctgaggta ggcaccatcc tttctatttt 65160gcaggggaat actaaggctc agaggggtta agagaccttc ccaaggtcac ccagcagggg 65220agacaccaga ttgcaaagaa gcagccccca cccacctagt gaggccagga aagcagaggc 65280ataaatgtcc agctgagcat gagactcctt gggccaacag gagcacggtg gggtcagcac 65340cctcagacag gcaacctctg agggagcagc attaacccct gggaagccag ggtagcttgt 65400taatccagca aacctgcact tctcaagaaa aaatggccct ctgttgggaa ggaatgattg 65460agctctaatc atctagcagt ccctgggcag ccacactcac catctctata atgagggcag 65520aaatagaggg caagaggtat ggtaaccatc cagaggcagt gactgggact cctaaacccc 65580tccttgattc tgagtcccca ggggccagtg gaggggcatt gggaggtgag acaagacatg 65640cagacaaccc gcctccaaga gatgtcatag agaagtgggc ttcagacctt ggggtgtgga 65700agcacctgga gaataggtga aaacgctgat gcttggcccc accctgaggg atttgggttc 65760atttggtctg gggcgaagcc cgggcatctg tgggtttttt tttttttttt tttttgagac 65820agtcttgctt tgttgcccag gctggagtgc attggcatga tctgagctca ctgcaacttc 65880tgcctcctgg gttcaagcga ttctcctgcc tcagcctcct gagtagctgg gattacaggc 65940acctgctcac acacctggct aatttttgta ttttgagtag agacggggtt tcaccatgtt 66000ggccaggctg gtctggaact ccagcactca agcgatccgc ctgcctcggc ctcccaaagt 66060gctaggatta caggcatgag ccaccgtact ctgccaaaaa tatatatctt tagattagct 66120tttaatagag ttcttcaaat acccaaaaga ttagggaaaa ctaggatgct aaaagatgaa 66180ataggtgact aggcaaataa atcattaggg aaattggttt caggctcatg gtattgttcc 66240ccacaatgct gggcaagtta cttgtccatt ccaagtttca ttttccccta cctgaacaat 66300ggagctaata aaattgattt caccgggcgt ggtgggtcat gcctgtaatc ctagcatttg 66360ggaggccaaa gtggggatgg atcacctgag gtcaggagtt cgagaccagc ctggcccatg 66420tggtgaaacc tcatttctac taaaaaaaat acaaaaaatt agccgggcat ggtgtggcat 66480gtctgtagtc ccagttactc gggaggctga ggcagagaat tgcttgaact tgggaggcgg 66540aggttgcagt gagccgagat cgcatcactg cactcagcct gggtgagtgc agagtgagtg 66600agactctgcc taaaaaaaaa aaaaaaaaaa tttcacagtg tagttttgag gacgttgtaa 66660gagggcgtga gttatgtgcc aggtgggtgg taaacgttca atagatgcta ctttccttcc 66720ttcctgagca tttggaccac acagagacct ccctgctctt ggctttggct ttaaggagct 66780tgtggtcact gagaggattt tgcactggaa gtacatgcat tcaaaatgga tacctaagtg 66840tatattttct ggtgtaaact atatgtcgac tctaacttta gcccggggga gctttattat 66900ttgtctccct ttcatgaaag ctataataga ggaagagaaa accctgcctc gcacattccg 66960attcctaaat acataattta taattttctg ggatattatt taagtttatt ttagttctgg 67020atacacacca tccccgtggg gtgcttattt aagtatcggg tgggctctgg aaaggcctgg 67080aatgcctcaa aaggagggaa gaagattctt tccattcatt aaacagcagg gcctggggtc 67140tttgcaggtt ctagggctta gaagtttcct taacaccaac tgaggagtcc cagggtggga 67200gctgagctca cacgaagctc tacttcgcgt gtcctgaagc tttgacagtt gggcctcttt 67260ctggcttttg catcctctgc tcatactaag gccagcagag ctacaggttg gaccatggtc 67320tggccgggag ctccagcttc ctcttttcct ctcagtagtc atcgggccag ctgcccatac 67380ctggtgccca ggtatgagaa gagacctttg gctttcacca ggttcttgag agggtcaggg 67440acctccaaaa ggataaatgc cattggtcag atagtcactg tctacctccc tcccatccat 67500ttcctgcctt caacccctgt tcttgggaca aggccttcgc tggcattcaa tggcgggtcc 67560ctgaggaagg ccccagcctt gccgaacatc cttcaaggga gacttcagcc cctggccctg 67620cagtgagtgt gttgttagca gcagaggggc tgagtgaggc agtgggatgg ggcttttctt 67680ttttgttctg gttgcaaatt ataaaccccc aaatgctgga gttccagact gaggttcaca 67740aactttagag ggcagaacta aagtcaaatg tgcttttaaa agcaccccag gagatgctga 67800tgtgggtgga cacttgaaga aacactggtt gataaagttt ctctctttct tttttttttg 67860agatggagtc tcgctctgcg gcccaggctg gagtacagtg gggccatctc acctcaccac 67920aacctccacc tcccgggttc aagcaattct cctgcctcag cctcctgagt agctgggatt 67980acaggcgtgc gccaccatga ccagctaatt tttgtatttt tagtagagat ggggtttcgc 68040catggtggcc aggctggtct cgaactcctg acctcaggtg atccgcctgt ctcggcctcc 68100caaagtgctg ggattacagg tgtgagccac cacgcccagc catggttgat agtttctaga 68160aagctgttcc aagttactgg ctgtttttga attgttcaag cttaaaaaaa agtaggcacc 68220ctttaggaag gccagtagaa gtcccattcc caaggagggc aggcctgtga gcacgttaat 68280ggcaacagtg gcagctaaca accacttttg tagccctact gtgccccaga cactgttcta 68340agcactcata aacattaatc taaacctccc aacagcccca catgccagga cacccgtgcc 68400tctatttcag gaaaggccat ctggatcttg caggacggca aatgggagag gacaaacaga 68460gccccatagg tgaaaaggag gaacgagagg ctggagagga atggggagca ttagagatca 68520caaaatgtgg tagacagaat aatggtcccc agatgttcgc atccacatcc ctggaacttt 68580tgaacgttac cttatcttac cttatgtggc aaaagggact ttgcaggtat gattacgtta 68640agaatcctga catgtgaaga tcattctgga ttatccagat taatccaggt ggattggggg 68700aacccagtgt catcacattt aagggtcctt agatgtggaa gataggggta gaagggtgag 68760tcaagggagt gccatgagag aaggtggaag gggtcacaag ccgaggaatg caggccactt 68820ctcggatccg caaaaggcga ggatatgtgg tctctccaga taggagcaca gccctgctgg 68880aacctggatt ttagccccat gagacccatc ttacacttgt gacctttaga gctgtaaggt 68940cataaatttg tgttgcttta aggctctaag tttgtggtaa tttgttgcag cagccataga 69000aaactagtag aaggaagaaa aggagcatta agagagattt cccactttgc tttgaaaagt 69060ggctagaaat agatggggaa acggggagaa gatggcccag agcggaacgg gggagaaagt 69120cttcttctat gactaaggaa gagtgaggct tctaaacggc cctcaggaaa gtgtttgtgc 69180catgatgctt ttcaggagag agtagagatc aattgatctc tgtgctgtgt tcttacatga 69240aatatttggg agggaagtgt tgtggtgagt cacttgtatt tttttttaaa tacctgccac 69300tcccagtttt ccagatagct gaggaaaggc tggggttcaa atcccataaa aaaccaacat 69360cattcaattg gtacattctc taaaatgtac ataagacatc taggaggact tcataatgat 69420taagagcaat caactgttaa tgcagtgagt gcttattttt tccaggcact gtaccaggca 69480tgctgcttca tttaaatttt gcagggactt ttgaagtagg tcctaccttg atttccattt 69540tgcaagtgaa aagcatcaca ttgagatgtg gcccttcagc cttagccctt tgatatggtt 69600tggctctgtg tctgcaccca aatctcattt tgaattgtgc tgccataatt cccatgtgtt 69660gtgggaggac ctggtgggag ataattgaat cgtgggggca gtttccccca tactgttctc 69720gtgatagtga ataagtctca cgagatctta tggttttatc aggggtttcc actttagcgt 69780cttcctcatt ctcactttgc ctgctgccat ccatgtaaga cgggacttgc tcctccttgc 69840tttctgccat gattatgagg cttccctaat cacatggaac tgtaagtcca attaaacctc 69900tttcttttgt aaattgccca atgtcaggta tgtctttatt agcagtgtga aaatggacta 69960atagaccctt cttcctcctt gctaaaatac ttttttgatg gtgatcatga attggccttt 70020gcgtgcagtg agttttgact ggtctgccaa tgcctttcct ttccagctat gaatggtttt 70080aaaaaatatc aatgacaagg ccccacctca taaccattaa atgcaaaact ctggtagggg 70140cccaggtgtt tcttttcttt tcttttcttt tctttttttt tttttttgag acggagtctc 70200tctctgtcgc cagggtggag tgcagtggca caatctcagc tcactgcaac ctccacctcc 70260caggttcaag agattctcct gcttcagcct cccaagtagc tgggactaca ggtgcgtacc 70320accacgcccg gctaattttc tgtattttta gtagagatgg agtttcacct tgttagccag 70380gatggtctca atctcctgac ctcattatcc tcccacctcc gcctcccaaa gtgctgggat 70440tacaggtgtg agccactgtg cccagctggg cccaggtgtt tcttaaaagc tctcaggtga 70500ttctaatgtt gcagccaggg atgagaccat ttgtctaagg caatctggat aactcctttc 70560ccattagcca gggattagcc tgtgaactat tctgaccaag gaggcatgaa cttttctgtt 70620tctggatgtt gtcatctgca aataacagga aggaacctag gatctgaaga tgctgctgag 70680ctgcctgagt agctcacctg gatccaccct atttctagac ttcttgttat atggtgaata 70740aatgtcctcc tggtttaaga caagggttgg caaactatga cccaggagcc aagtctggcc 70800cactgcttga ttttgtaaat aaagttttat tggaatacag ccatgcccat ttgtttatgt 70860attgtctaca tattgtttag tgctacaatg gcagggatgc atagtcatga cagagacctt 70920atggcctgca aagcctcaaa tatttactct ctggcccttg gccctttgca gaaaaagttt 70980gccagctcct ggtctaaggg tctttttgtg ggcctttcta ttacttgcag ttgagggtat 71040ctgttaattt gggagtttag cttccccaag gtggcattgt tagtgatggc aagttttgcc 71100ccctttaagc cattttttca tgggagaata attacagaat gatctttaag ggatttcaat 71160aatatcagcc aatcctactg cccatgggga agtgggatca cacaggttgg ggcttcagat 71220aggcctggat ttatggcctg gttttatcat atagatccaa ttgtctttga aaagtgactt 71280catcttctag tcctcaactt tctcatctaa aatgaggaca gttatatcta cacgaaggat 71340gggtaccctc atggaggcaa ttccagacaa aggaatcagt gcaagcaaag gaagcgaggt 71400gaaaaatctc caggtccaca tggagagccg caagctgctc tgcctgacct aagccaagag 71460catagaaaag gggtcatggg gcacactgtg gtgaagatag gctggcattt gttgcataat 71520aatttctcca gttgtcttgg gtttctgaga ggtagcttct aaacccttgg aatttcccaa 71580gtgataggaa tgtctttgtt attcatagtg agccactggg accatgcctg agttcatact 71640aacaaggtga ctcatggtgg gcccctagat agtttcaaga tggggactag ccattaggaa 71700ccaaccaaat gattagaggg ttggggtttt gagccagatt atatcagcct gacttccagg 71760gaggttagag attgagttca atcacatggc caattatttg atcaatcatg cctcgtgatg 71820aaacaccaat aaaaactctg gacctcaagg ctcaattgag cctcctggtg ggtgaacacc 71880agtgtgctag gagggtgata catcctgact ccatggggag aagatatgga agctctgtgt 71940ttgggatcct cccagacctc accctatgga tctcttcatt tggctggtcc tgatttgtac 72000cctttataat aaaagtgtaa tggtaagtat agcactttgc tgagttatgt gagtcattgt 72060agcaaattat caaatatgag gggttgtggg aacccccaaa tttgtagcca gttgatcaga 72120aatgcaggta gcctggggac cccatattat gtggggtctc tgaagtgagg gcagtcttgt 72180tgaagactat gtccttaacc tgtggagtct gtactaactc caggtagttg acactgcaaa 72240agtatatact gctgtattgc agagctggat tcatgaatgc ctgtttccag acttggattt 72300aatttggttg gtggtgggga gccatagaag gtttatgaga agatgaatga caggatcact 72360aactgagatt ctggaagaat aattgggcag agataaaggc tatctagagc tctccctgag 72420tcacaggatc ttttggtcat gcactctcaa ctttgacatg tgatgctggt caccaaagat 72480gcttcctttc aaaaataaac agcagctata aaggaactca catatattgg tagggttgga 72540gccatcccag ggcaggccag gcctaggagc tacaggaaat cctcacttct ggaagcagaa 72600ggtcagttgc ttcttcctgg accacaccct ctgcagacac tagggctcat ggaggagtcc 72660tggagggata cacagcccaa gcctttgctt tccaccgtaa gaggatggca gtagaatctg 72720ggtacagctt cctcttggag gtttggggaa cccagtgaca ttcaggagcc ccctttttta 72780aaccttctgt gccctccagc ttgcttcttt ccagcaacag ctagccactg gggcattgta 72840tttcagcctt tctggggcat cccctgcaag ataggcaccg gcactgcctg ctctctctct 72900ccctggatct gatttagcct tcactgaaac cctgcccaag atgatgactt aatctctggg 72960ctgagcgaat gcagccaata tgttcagatg gattttgcaa gacaaaagct caaatctgtt 73020ttgtggctgc agatacacat tcagactgaa ccacatccgt ggtcattcca gtcacaaaca 73080caaagggtgc catgaattct ttccatgaaa aataatttcc attgtattgt ttctgtcatc 73140attcatttca tgataagtca gttaaaacag ccggtccgag tctgtcattg attccaagag 73200ccccattgtc gtgtccttaa cttactgata gttttaggtc ttaattgtta gtagcaaagt 73260ttcttttcag gaattctgtt ttctcattga ctttgatgag aaagaaaatg tagagagatg 73320ctagcgaaaa aatttaattg ccctcaaaga agtagcaatc ttttttcttt aatatatatt 73380atgcatcaag caaaatcatt ttctgagaac tagttccaga ttgcgtccaa tgtcatgggt 73440tgtgtgtctt atttatttat tttctcattg agatataatt cacataccgt aaaatttacc 73500attttaaagt gtacaattca gtggttttag tatattcaaa agactgtaca accatcacca 73560ctacttccag aacattttca tgacagaaaa aagaaacttc gtcctcatta gcagtcactc 73620tccacttcca ctgtcttaca ttccctgaca acaactaatc tctcttccat ctctatgtac 73680ttgcctattc tggacatttc atataagtgg agtcatataa tatgtggcct tttggtctgg 73740cttctttcac ttagcatgtt ttcaaggttc atccatgtcg tgcgatatat cagtacttca 73800tttcacttca gaattgcata atactctatt gtatgtgatg cagggcagat gaaccccaaa 73860ttgggactta gtccatgagg atttttggct ttgcccagga aggaattcaa gggcaagcca 73920gaggtagaag acagctttat tgaagcagca gtggttacag ctctgtgact gctcctgcag 73980aacagggcta ccccataggc aaagagtagc agctcagggt attttgcagt catatttata 74040ccacttttaa ttacatatgg attaaggggt ggtttatgca gaaatttcta gggaaggggt 74100agtcactttt gggtcagcgg gtcattgcca tggaaaggag tggtaactcc caggtgttgt 74160catggcaatg gtaaactgac atggctcact ggtgggcata tcttatggaa agctggttct 74220gccccttcct tgttttagct agtccgcagt ttggtgtctg acttcatctc tggagtcgag 74280tctcacttcc tacctcatat ggatatacaa taatgtgtat atccattcat caattgatgg 74340atattggatt gtttccaatt tttggctatt atgaataatg ctgctatgaa cattcatgtg 74400caagtttttg tactgacata ttttcaaatc tcttgggtat atacctagga gtggaattga 74460cgtgtcatat agtaaactct atgtttaacc tactgaatta gagtatctgg gactacggcc 74520cagcaatctg ttttaacagc tctccaggtg attctatgat caccacaatt tgagaggcac 74580tgccctagag taaggggcag taaacagcta gatagtaaat attggggttt gcaggccaag 74640agtcaaaact gaggatgtta ggtaagcact tacttgataa gacggtaaac aaatttccaa 74700acatttttat tgacaaattg aaaaatataa taataataat tggtaacagg gttgtttctg 74760tttgttggtt tttggttttt tgagacatgg tctcactctg tctcccaggc ttgaatgcat 74820agctcactgc agccttgacc tcctgggctc aagtgatcct cccaagtaac ttggagctac 74880aggcatgcac cagcacactg gctacttttt acattttttg taaagatgag gtctccctgt 74940gtcgcccagg ctggtctcaa acttctgggc tcatgcgatc ctcccacttc agcttcccaa 75000agtgctggga ttataggcat gagtcactga gtcctgccag gaacagtttt ttgtaatgca 75060ggtttactaa tgagaagaat gtgtttggtt tttttgtttt tttgtttgtt tgttttttgc 75120tttgttttgt tttttgcagg attgggaggg caacatttta cttaattggg attcaaagtt 75180ggtgttccct gtcatcaaat caattgcaga tattcatctg taaaaaccat tcttccggcc

75240agcgcagtgg ctcatgcctg taatcccagc actttgggag gccgaggcgg gcggatcacc 75300tgatgcaggg agtctgagac cagcctgacc aacatggaga aaccccgttt ctactaaaaa 75360tacaaaatta gctgggcgtg gtggtgcatg cctgtaatcc cagctacttg ggagctgagg 75420caggagaatc atttgaacgc gggaggtgga ggttgcagtg agctgagatt gcgccattgc 75480actccagcct gggcaacaag agcaaaactc tgtctcaaaa ctacaacacc aacaaaaacc 75540attcttcctt catgggctgt aaagtaataa gccgtgggcc agatttggcc tgtggctcta 75600tggtttcaac tagttctggt gcatagcagc ccccaaagtt ttagcagcgt ggttagtgga 75660gagctgtggc ttttgcctcc atctaaattt ttctttgagc aaattacctc tctcctgaca 75720gccctgttgg gggtgtcagt tgtggggttc tggtacctcc catcccggcc ccagctagag 75780caggcaggcc cacaggaatt tgaatctgga gggagttatc agtggtggag cgtagaggct 75840gcaggaatct tcatataagt ggagtcatat aatatgtggt cttttggtct ggcttcttta 75900cttagcatgc tttcaaggtt catccatgtc gtgggatata ttagtgcttc atttcacttc 75960tgatggcagg gtcctgaatt aagtcctgcc ctgaggatca cactgttaca tctaggcctt 76020gtaggattcc tgaaaattgc atcccatctg ccctgctcta aaatctacac agcctttgcc 76080atatccattt atcgatttgg ctcagcaaca tctactaatg atgcttaaat cctgggcaag 76140tgtatttgaa gtggccccag aggttaaaga tctggatgtt ctccctccag caacagcacc 76200actgccccac agcctgggga ttttctgttc tgcttgctat taatcactta cttattgcat 76260acttatatgt gccaaaaatg ggatcctctc ttgccctatt gtaaagtgat acccatcttc 76320tagaaaccaa tccagcaata tatgagaaga gccatagaaa tgtgccccct tggggaaaag 76380aaataatcct aagtagagaa aaatgtattt gagcaaagtc attcatggcc acattctgcc 76440cttccgtggc cagaaatgga taaaagctga atctactgcc agcaagtatc acctccactc 76500attcatgatg cagagcgtcc ccacccttcc cactgggttg ccttccagaa aacagcttcc 76560atgatgccca gtaatctctg tttcccttga tctctctcat ctccttcctt gtctaacctt 76620cttctcttag aaaaggagct tcatcccctt tggtcagtag ccagcaaatc tggattgctc 76680cctgtggccc ttgtgctcaa ggcaaataga accacctgct ccacatcctc ccatcttctg 76740agtggagggg ccttgagata atccacttct ctgcttagag tggattcttg atgtatcact 76800ctaatgattt atgaggttaa aagactccct cctcagtagg ggtgagctta aaatgttccc 76860tcctcagtaa ggatgagcat agagagggaa aaggagctga cagaaccaga gaatcccatc 76920caccacattt acgatagtga aaacattaga agccatccaa tgttccaaac acctctggta 76980ccagccaagg gggctgaccc aaggcaggat cccaggagct agggttgagt gggtggggga 77040ggcactccag gcaacaaggc atcagatctg aggacaggtg ccaggctcag cctgacaagg 77100taggccaagg gacagttcta gagaagcagc caggataacc tgttttattt tgtttttaat 77160tgggtgcaga cacaagggga gtatggaaag ccattggctc aggtctcaat gggtctcaaa 77220tttagacttc tgctattttt ttcttcaagt gcaattgcat atttagtctc agagagtgac 77280tctgacaaga cccaaagaga acatttgtca tttggccttg accttgtctc cacatctgga 77340gagttggtat cacgtgtggt accttatttg tacatttctt gggggttcat aacaatgtta 77400taccacatta ctggtgaaag gaatattaat ttgttcagtg taaacactga aatagctgac 77460aatcttttat ttttaatttt aatctattta tttgttttga gactgggtta agagattggc 77520taatatttgt attttgggta gaggtgggat tttaccatgt tgccaaggtt ggtcctgaac 77580tcttgggctc aagaaatcca ccccccttgg cctcccaaag tgctgggatt acaggtgtga 77640gccaccacac ccagcctaaa atagctgaca atctttataa ccctctttag gcatatttac 77700attttatagt atattaggaa cctcaaaaac tgatggcaat tcaggttttt ccagtattca 77760agaaggcatt atctaagctg tcagatctag gggggtggtg agtgtgatac ataaattggg 77820aggtaaatca aaaagtctgg agcaggcaga agaatgcagg aagaggtcag ggagtgaagg 77880actggattga gagggccaag atttggggtt tgaacagggc tgacttcagg gagcgggagt 77940ctggccccag gtaaaactta gtgtcattgt gtgggtcaga agacaacaaa ggatccttaa 78000ggaaactatg gcccatcatc tcagtagacc aggcagcctt tgggaatgat ggtcacaaag 78060actctgtggc aacatagaaa atgctaatgg tgtcatacag aatacagaac tttatggacc 78120tatggtacaa acaaacacat gcatgtgcct gcacacacac acacacacac acacacacac 78180atacacagag gaaacaagac tggaaggggt tacacctaaa tataagtagt gcttgtgtcc 78240atggggtgga gtgatattgt gggtttattt gctctgtatt tttaaataaa acaatggctc 78300aactcagaag ataactgagt cagcgtctca tccagcagcc cccggggccc ctgggagaag 78360ccacctccca ccacctggcc aagaagatct agacaggctg agaggctccc tctccctctc 78420ctggggtccc gagccacaaa tctaaagcca cattccttgg gtgaccaggc agggactggg 78480gacagatgtc gaaactggca aggccccact ctgtggtacc cagcctgctg tggctcctgt 78540cctagtgcca gctcctctgc tgtcatcccg tccccacccc acacatcccc acctccccag 78600ccagttgggc ccaaggtgcc ctcactgata tgttggagga ataatctgct tctctccagc 78660tcctcaggag acccgcgtgg gaggtcccgc gtcacgggct actgaggcac atgctgagtc 78720ccctcccatg ggcctcagca cacctactat ggcttagctc ttcccaccca ggacctcctc 78780agggcttctc ggcattccag aaagacaaga agattttctt ggggccaagg cgttgctggg 78840cctctctgtc ccggccaggc cctcggcaag ctccatgcct cgactctact gccttctctc 78900tggtcctctc tgtcaccctt cagagacttt taaatcccat agtctgggct tcagggcctc 78960caataaaagc agaataagac ctataaggac ttaatagagc ttttatctat aaaagagaca 79020gaccttctaa tctaactaga aactaaggtt aaaaaaaatg aaaagagtta gacttaaact 79080cttaaaaaaa taaaaaaaga ttcagctctg tcagagtcag cctgatgtgg tagacaaatt 79140gctcatgaat tactcacagt gtttgctaaa aaatgctggt tcctgggagt ctctgagcct 79200actctggctc tagaggctgc ccaatttaaa aaaaagaaaa atgcaggttc ctgagtctag 79260ctccagaact accaaatcaa aatctcgggg aggtgaggac tgggaatctg aatgtgtaac 79320acgttcccta cgtgattctg aagcacactg caccggtggt ttagtggttt ccatgccagg 79380agggcattgc gccctccttg agaaccctgc cttcccagca ctagctgctg ggaggctggc 79440cagcacagac acagtcctcc ttcccaagag attcctagcc ctggcgagga ggaagactcg 79500gtgggtaaca ggagttgcaa tgtgggtttg tacccagttc ttggggagca cagaggagag 79560gggagttctc agagagggaa gatgtctcag ggaatgagtc tctggggcca gatcctgagg 79620gctcagtagc attgactagc taaagaagag agcagaggta tcccaaattg aggacatagc 79680atgggcagag gcacaatggg gagctgtaaa cagtttaggg cctgggcaca ggtagggagt 79740agaggggagg aggccagaga gatgagtggg gctggtcttc ctgctggttt gtgttttgtc 79800catttggcta atctgggaat aatacttccc agaaacccct tctccagagg ggtctagggt 79860agagttggtc aaaagaggag cttgcatgag atttggaaaa taaaagtgaa gcagcatcct 79920gactctcagg gggttgtcac ggtcatgtgc cgtgatgaca ctgaggcaga gatatgcctg 79980gccaatccac ctctcgggct gctggctccc ggtctggccc gtgttctggc cagctgggct 80040tgctgagcta cagctgcccg cagacctgtc caccggctcc cttcacggcc tcactcaggc 80100agctgatgtg cttgcttctt gggcttccct gcaaactctg acttgtcacc cgtattaggc 80160cattcttgca ttgctataaa gaaatatctg agaccagatg cggtggctca tgcctctgat 80220cccagcagtt tgggagccca aggcaggagg atcacttaag cctaggaaga ggaagttgca 80280gtgagccaag attgcaccac tacactccag cctgggcaac agaggaaaac tctgtctcaa 80340aattaaaaaa tttaaaagaa ggaaggaagg aaggctgact gacctgagtc tgggtaattt 80400ataaagaaaa gaggtttaat tggcttgtgg tcctgcaggc ggtacaggaa gcatggtgct 80460gggcatctgc tgggtttctg gggaggcctc aggaagcttc caatcatgtc aaaaggtgaa 80520gggggaagcc agcacctcac atggagaggg ggagcaaggg agataatggg ggagaggagg 80580gaagtgctac acgccttttt ttttcttaga cagggtcttg ctctgtcacc caggctgggg 80640tgcggtggtg caaccacagc tcactgcagc ctcaaactcc caggctcagg tgatcctccc 80700acctcagtct gcctagtagc tgggactaca ggtgcacacc accattcctg gctaattttt 80760ttttttttgt attttttgcc atgttgacca ggctggtctt gaactcctgg gctaaagtga 80820tccacctgac tcagcctcct aaagtattgg aattacaagc atgagccacc atgcccagcc 80880aggagtccct ttttgttctt tttttaaatt ttattttaga cagagtttca ctcttgttgc 80940cccggctgga gggtagtgga gcgatctcgg ctcaccgcaa cctctgcctc ctgggttcaa 81000gcaattctcc tgcctcagcc tcccgagtag ctgggattac aggcatgtgc caccatgccc 81060ggctaatttt gtatttttat tagagacagg gtttctccat gttggtcagg ctggtctcga 81120attcctgacc tcaggtgatc cacctgcctc ggcctcccaa actgcttgga ttacaggcat 81180gagccaccat gcctggcagg agtccctttt taagagcagg aaaaatattt tccataagca 81240actcctccaa cacacacaca cacacacaca cacacacaca cacacacact cagagtccct 81300ccatggctca tcggggtaga ccttttaata tccagggctg gagaaggaca atcccttcac 81360acttgaacgc tgtgaagtct gtgcccttgt ccagggcacc tgccaggagg agtaggcagt 81420gggtccggtc tttcagtgga gggcttctgc agcttcagat cgtctctagg agtctggagg 81480tctatcttat ggcttgtatt tctggaaccg agtcatgcct gcttgaagat aagaagatca 81540aacaacagga tgctgtggct tctcctatcc caggggcctc gaggacaaat cagttccctt 81600cctgggggag cagatcctcc tggctgggat cttcagtcca agttggttca atttatatcc 81660ttcacgtctg gcttctcctg catgtccaag tcacaggaat tttaagtcag gatcttctct 81720gcacccctga gcatgacagc cagcctctgc cctcatgaag ctcccagtct aagggaagaa 81780ataataaatt ggtggccggg catagtggct catgcctata atcccagcac tttgggaggc 81840caaggtgggc agatcacttg aggtcgggag ttcgagacca gcctggacaa catggtgaaa 81900ccccatctct actaacaata caaaaattag ccaggcgtgg tggcatgtgc ctgtagtccc 81960agctactcag gaggctgagg caggagaatc gctggaacct gggaggcaga ggttgcagtg 82020agccaagatc atgccactgc cctccagcct gggtgacaga gcaagactct gagattatta 82080tctcaaaata ataataataa taaaataata ataataaatt ggcagttaga gggcacttca 82140ccaaggcaat atgagggaaa ctacaatctg tcaggaaaac acaggggaag gccctaactg 82200gcccaggagg ttcagcgggg catccaggag gatgtatgag agccgaggag ggagtagaag 82260gtgatgtagg aaaaccactt agctgaaaga ctcgctgccc aaaggccgga ggtgggagag 82320agtgcgggac ttgcaggaac taagaggagc ccagagaggc atctgagaag agaagggctt 82380ggagagctgg gagcatcaga tcgggtttag cctgggggcc tggctgaggt gagtgctctc 82440catcctaagg ccacaggaag cagaggaggg acaggatcag attctgcgcc cagaggaaga 82500ctggcttaga ggaggtgagt ccagaggcca ggagaatatc agaggccagg acaagggaga 82560cggcggccaa accagggaag tggcactgga gagaaatgga tggattcaag agacgttagg 82620caaatggaca gaaaacagaa accgcgcacg tggtggttgg tgagttgaat gcatgcagga 82680gataggggcc caagggagaa ccataggggg ttgattccaa gggtctggtg gagcccctga 82740ggggttggag acacccagct ggtcagccac acagtctggt attaaatgac acacggctgg 82800tcagccacac agaagctggg cagtgtgaat gggtccagta ccgacttcag agtgtgcacc 82860tctgtctccc cagacagcaa cagaaggacc agggaaattt aaaagggagt ctatgaatga 82920tattgtaata taaaatctct aatgaagatg tgactactag aaaaaaggga aataaagcga 82980ttgaggaaag gaaaacaact tgtgcataaa gaagcaaaga ataagcaact gaaagctcaa 83040aacatttttc caggcctggg ggatgaggtg gagagagtct cttgctccca ctccaggagg 83100agggctgcct ggaactaaag ttggtgctta tgatctggta tttaaatgac attcttagtg 83160gtggtgttag cggtagcaga gcaattgtgc cagggcccac cgaccccttg ctggttcact 83220cagttgccaa catatactga tatactgagc acctgctgca ggccaggctc tgtggtctca 83280gatctcaaga acaagccagc tcagtccctg tctaatagag cttacaactg ggcagcggag 83340gcagacctgc catgagacaa atataaaatg acaacttgtg acaagggtta ggaaaaagaa 83400tagtggaatc ctgttttaga tgggatggtc agggaaggcc tctgaggagg tgatatttga 83460gccgacatca gatggatggc agagaatgca agaagagtgt ccctggcaga gggaacagca 83520tcgtcataat tgggagctgg tgctttcgag gaactaacag acaggcaggg tgggtggagg 83580gtgggagcca gggctgggct gggggttgtg aggacactga caagagacca gttggagagg 83640gggcgggggc tgaccatgca ggaattggaa tctttcagta aagtccagac caaatgattc 83700ttcagggtcc ttgttccccc aggtttcaat gacactttaa aggtgttgtc gtaaaaggct 83760ggggagtctg tttctatgcc cccaatctca tttaagaagg ggggcatcca ggccatcatg 83820gaacaggtga cctggaagat gttccttggt ccagtttctg caaactgccc cgcagcattt 83880ttagaaaatg ttccctttta gattcgattt atcttagcca aattgaccag ggaaaatagg 83940tgcctacaaa tagcgatcac tggcaaacaa ggagagttat tatcttaaat taaggctggt 84000ttctaacaac aaaaaaaaac ccaccaaatg ccactggcca ccccccacca accccgatcc 84060ccagcgcata cgtgaggacg atggctccac caccctccct gtaagtacca ggctcaatgc 84120cgggctctgt gcaaagggaa ggaaacagac agaggaagga aggcaagaga ttagagaagc 84180tgacagatgt aaatagcctc agaggagcca cactgtcccg gcatttctcc ccagggagcc 84240ttttgtacca aggaatctgg ttgcctgaaa gaaaaatggt atcatttatt tctttagtca 84300gagctgagct gttttcttca gacatagaaa taacctaaca tcacacacca aattgttggc 84360caaatgacag agatacctgt aatgtgggtg tttaataatg tccagggtaa acaatcatgg 84420acttggtttc ttgggaaggg ccccgttccc cgccatggtt gcaaagctac agagggtctt 84480gaatgaaata agtgctttgg aaaagttgtc ccaaagttct tgcctgctgc tgagctggga 84540ggaagctggg cttttcattt ctgctgctca aatcatgccc cagcagccaa acatccatta 84600attcaacaaa tatttatgga gcccggattc tggccaagcc ctgtgcaaaa gcactgaggc 84660tacaaatgtg cagaggctgg ggtcctgtcc tcaatagcct catagtccag aggggagacg 84720gccacgttca gcctgtgtgt caatcttgta acacagcctg gatgtggcgt ggtggtcagc 84780tctgtggagg aggcagtctc ctcaaaagga gacttgaagg ggaatttcca ggccagagga 84840gttggaggag gggatcctag acagactaag gagaatgtgc aaaagcagag gcttgaaacc 84900ccctgggcga tttagaaatt aagagaaata cgatattgca gagaacaaag tgctaaggaa 84960gtcagcggtc aaatgcgtgg ctggacaggt agatggatac cagatcatgt acttgtaagc 85020taaggagttt agagttgacc ttttaggtaa taaattccat ggaggaattt gagcggggaa 85080atagcatgat tagatttttg tattagaaca ctcactcctc tctgtcctga agaaactcta 85140gcatatgtcg cgtcaggaga caaattcggg catcttcctt agtgcattgg gtttgatggt 85200gaaaacactg gaaacaaact aaatcagtag aaaaatagat aagcaggcca ggcgcagtgg 85260ctcacgcctg taatctcagc actttgggag gccgaggcag gtggatcacc tgaggccagg 85320agttcgagac cagcctggcc aacatggtga aacctcgtct ctactacaaa tacaaaaaat 85380tagctgggtg tggtggcaca tacctgtagt tccagctact caggaagctg aggcaggaga 85440attgcttgaa ctcaggaggc agaagttgca gtgagccaac attgcgccat tgcactccag 85500cctgggtgac agagcgagac tctgtctcaa aaaaaaaaaa aaaagaaaaa tagataagca 85560aatagtggta tagtcataaa atataatacc attcaagatt ttaaaataat ccaacatggc 85620tagatcttaa aagcataatt ttgagtaaaa aaaaaaggtg agtttcagca tatgtatggt 85680atgataaaat gcatgtgaaa tttaaaaacg cacgtttgta tattgattat aaataatata 85740tgtaggatgg tatgaaaaca tggattgttg cctttggaaa caatccatgg agggaaaggc 85800atggaaagag aaatacatag aatttttcta ttggttttat atctaaaaga aatatgggcg 85860gggcatggtg gcttacacct ataatcccag cactttggga ggccaaggca ggaggatcac 85920ttgagctcag gagtttaaga ccagcctggg aaacatagtg agaccttgtc tctaataaaa 85980ataaaaaaca ttagctaggt gtagtggtgc acacctgtag tctcagcttc ttgggaggct 86040gaggtaggaa gaccacttga gcccaggaga tagaggctgc agtgagctat gattgtgcca 86100ctgtactgca gtctgggcaa cagagtgaaa ccctgtgtaa aaaataaaga aatactgtag 86160atggagctaa aatgctaacg tattaaattt cctgtgttta aaatattttt tgaactttgc 86220tttttaaaaa aacagagaaa gtaaaatcca tctggcagcg gcatagcaga tggaagggag 86280gggattgggg aatgggaccc cgagccagtc tctgtctcca cacagcagct cctgggtgga 86340ggagagctgg aggccgatct tgggggaccc agggagtaat gcatgcagag aacacttcac 86400cttgttgggc tgcttttggc ctctgggcag agcggaggcg tggtcctggc atcttgtcta 86460gtgtccctgg aagatacggg gctctgtctt gatcggaact ggcgaccatt ctgacctggg 86520atgtcagtat catttttgga catctgccaa ccaagggtca gaaacaggag gattttagaa 86580tacctgggaa agcttggcat tgtatattca gagtaaagat ggtgtgaatg tgtgtgtgtg 86640agtgtgtgtg tgcatatgca agcacatgtg caaatccaga agcacagtga aggtgagtgt 86700tccccaaggg ggtttgcagt ggatgcagtg acgaatacat tcactgatga ggatgctctt 86760ctatagttca gagaagtaag aatgaatcat gaatctttcc agaaggaatt catttctaga 86820ctccttctta cctttcagat ctccatcgcc tgacctagag tctcggctga atagcataaa 86880cctgtatcca tctacatata tttatacatc tggcttagag ccacagcagg gaagaattca 86940cagcaggatc ttataggcac acagcctata ggatcttata gattacagcc tggatcttat 87000aggcatccat taaagaaaac tacaggcatt tccgcaactg catatgaatt gtgtcacatc 87060tgtatgtgct agaaggagtt caagggcatc aatattccta ttgtaataca tctgcccagt 87120gctagcagca ttacttaagc aaacctatca aaagcctaaa gaataaagat gtggcggggt 87180gcagtggctc acacctgtaa tcccaacact ttgggaggcc gaggcggggg aatcacgagg 87240tcaggagatt gagaccatcc tggctaacac agtgaaaccc cgtctctact aaaaatacaa 87300aaacaaatta gccgggcgtg gtggcacgtg cctgtagtcc cggctactca ggaggctgag 87360gcaggagaat cacttgaagc caggaggcag aggttgcagt gagccgtgag atcgcaccac 87420tgcactccag cctgggcgac agagtgagac tctgtctcaa aaaaaaaaaa aaaaaagaat 87480aaagatgtgc accagcttgg gcaacaaagt gaagccccat ctctaaaaaa atacaaaaat 87540tagccgggca tggtggcaac tgcctgtagc ccagctactc aggagagtga gacaggagga 87600tggcttgagc ctgggaggca gaggctgcag tgagccatga ttgctgccac tgcactccag 87660tctgggtgac aaagcaagac tttgtctcaa aaaaaacatg tatagctaca taattaataa 87720tatgcaaata ttcttcttta gggtcactta ggttttcaac atacagaccc aagagaaggt 87780acagagactc attctctctc tctttcaatc acacacacac acacacacac acacacacac 87840acacccctct tcactataat tataattact atgttggctt ccacatcagg ggttagagcc 87900ttggcatgga gacgcctgaa aggcacccaa ggcaattagt ggtgtccctt ctccaccccc 87960tacatacctt caggcccttt gcacttgctg ctccccctcc cagtgcacac acccccagat 88020ccttgtgtgg ctccctctcc caccacattt tggtcttagc tcaaatgcca cctcctcaga 88080aatgcctggc ctggccgcct tcagggctgt ctatatgctc accagcttta tttttctttc 88140gagaactttc cattcccgga cattgtacta aatgtttatt tgttatccgt ccattatcca 88200tctccttgcc agcactccct gtgagctcca tgaggctcca agattacaac cacatgccca 88260gagctagggt ggtgcctggc tcacagctgg catccagtca gtagcaatgg aatgaatgaa 88320tgatcaccct ctcttcctct ccatccccac cctctccttt ttcacttcct ctcccgcccc 88380atctcccctg cctccgcctt aaatctgctg gcatgaccag ccctcaggaa gttgtcaggc 88440aggggaggtg tgtgctctgc acccctctcc ttccatctct ctttcccacc tcccttatgg 88500gtacctatct ctcctacctg cagctgctcg ctgccagctc tggccctctt ggtgggcaga 88560agcttaggca ccccagacaa ttgtagggaa attgggcatt ggagggcagc cgtaagaaag 88620gattcagtac ctgccaagga atgatgaatt tagtctctca cccatgaaca ggcatgcact 88680ttcagggctc aggttcggtc tctaaagcag aacgtcattc agggcaccct tcagtaccca 88740tgacccagtg tctcgccttt cctgcaggag tcggtgctgc cactatggtg actcagcccc 88800tctcttctcc tcattcatta cctgggttct tcctgcagtg attgacagta tcctttttct 88860tttgagatga agtcttgctc tgttgccagg gctggagggc agtggcacaa tcttggctca 88920ctgcaacctc tgcctcctgg gttcaagcca ttctcctgtc tcagcctccc gagtacctgg 88980gattacaggt gcatgccacc atgccttgct aatttttgta tttttagtaa agatggggtt 89040ttgccatgct ggccaggcca gtctctaact cgtgacctca ggtgatccac ccgccttgac 89100gtcccaaagt actgggatta caggtgtgag ccaccacgcc cagcaagttg cagctcttaa 89160tagcatgggc accatataat ttactgccca gattgggtca ttttttagag tagaagtggg 89220agctgttaat aattacgctt ggcctacagg tgtaaacgag accaccctgg gcaaaccagg 89280cacatggtcg cattgactaa gaggaatatt cccctgcctc ctgcatctcc agccacaaaa 89340ggagcctcag ctgttggtca aagctgccca atagctgagc ccttgcctgc ccttggacaa 89400gggagaagac agagggcagg ttccaagaat ggggtgggga tgtggcagga aggacggtgc 89460aggccccaga gctataatca atgatctggg agccgggttg tggagcacag ggctgtgtca 89520aggagcaggt gggaggtggg cgggtgtggg acatggcacc agcttttggt gtctgtgact 89580ctgaacttgg cttactcgca ggtggcttct gcaggctctc caccctccca gctgcgtaag 89640tcctgcctga ttcaaggaaa acagggaatt ttggggtcct gtggctcctc caacgttctt 89700ttcctttacc tccttgtgta aatgtggcct gatttgtact tggagcataa gtagacccct 89760acagagcgtg tgtgtgtgtc tgtgtgtgtg tgtgagagag agagagagtg agagagagag 89820agagagagag agagaaaggg acctatttcc ttctatccct ctgtctgtct tactctcaga 89880ctattaatac aagccctgag tctggctgta cccccagaac atgtgccccg ccccctacaa 89940caaaatgctg cccctcccag ctaggtctgt tgtttgttcc ttttctgatt ggcgccaggc 90000ttatagaccc catgtaggta gaatataact ttccataaat aacctctaac ccgacctaca 90060atttagcctt caggtttttt tccccctcgt ggtaatggga ttgcagcctg ggctgatcca 90120tcctgtatct tcaggtccca gaaagcagac cctaggtttg gacattgctt ggaattcctg 90180gtacccccat gttgccttgc acatggcaag gactcggtac atgttgagga atggtggatt 90240ctcttctacc catgagcagc catgcacttg cagtctttgc ttgggctatg ccttctgcct

90300agaagccccc ttctccacct ggaaaatctc taagcaacac ccactttgtg aagctttccc 90360taaccactat ccctcatccc ctccacagag caaattactc ctatcttgga ttcccataac 90420acttggtaca aaacagtgtg acctggttat tgcccatgtg cccaccaggt tctaagcagc 90480tcaagtgagg ggtagggcaa ggaactgagc ccaagtatgg ctgtgggcaa aacatttaac 90540cccttggtgc ttcagtctcc tcagattcaa acaccaacct ggggctgggt gtggtggctc 90600acgcctgtaa tcccagcact ttgggaggcc gaggcaggca gatcacttga ggtcaagagt 90660tcgagactag cctggccaac atggtgaaac cccgtctcta tcaaaaatac aaaaattagc 90720caggcgtggt ggcacgcacc tgtaatccca gctactctgg aggctgaggc aggagaatca 90780cttgaatccg ggaaggcaga agttgcagta agccgagatt gtgacactgc actccagcct 90840gggcaataga gcgagactct gtctcaaaac aaacaaacaa acaacacaaa cctgacccag 90900agcccgtgtc cccacccact ggaccgcact gcctctcagg gatggaagat gtctctgttt 90960cctacctgct ctcatattct gggccctgag acactcatga tgcatggatt ttctgcaggg 91020gctcactgag gcctcctcgg gggctggagc cccaggaagg gaccggccac tgatacccac 91080tcacacctat caaagacttg atgatgatga ttaattcaag tgggttattg gtagttaaac 91140tgacaagtca tcaggaggga cctcagcttt ctttgtgggg gtgggttccc ccaaggtagg 91200ctggacttaa tttaaagctt tccagttgac catcccacct tagggcaact gaagagtaac 91260tttagggtta ctcttcatct gaagatgcta actcagctag catccagcac tattaagaca 91320ccttggggag gcgagacttt gagagaagtg caggaggctg gcccactttt gaagatccta 91380catttctacc caggaggcat caaatccaca cttcgaagaa gatgaagttt cctgtgagct 91440ggtcctgaat tgtacttggc tttgtgttgt ctatgccagt actaagcagg acttggccag 91500gctggtcctc catgaatgac tgtcaaatgg aaaacagaat taggaaccaa agtagtggtg 91560tagacaaaag gggctggaac ctcagaggag agagggatca tgggagattg gaacatctct 91620ggaaggttag gggaagagat gacaagtaat gtgaatccta aagaaagagg attagttatt 91680gggggtgcta agtgtggatg gcaaactaag gaagcaggga tctgcatgac catttggagt 91740cagtaggaag cttggtggag tgggaggtgg gagtcaagca gagaaggcct cccatgtcag 91800atagggaaac tgcacttgat cctgggcaag ggggagctat ggaaggcttt tgagcagggg 91860agtgatacaa tgagaacagc attttggaag ccagcatgga ggggaggctg ctaaaaggat 91920acaaggctga ggttattacc atgccctggg tgaagaaatg cggtctgggt ttggaaagga 91980agggataaac atgacttttc aaaggaagac ataattgcca caagggccac cagagagcag 92040tcaaaggtgt gtccaaggtt ggagctctag tgaccaagag aagggtgtca actttgacag 92100aaatgacgtc atgtgtggtt gacaaaatga gaccacatc 92139251719DNAHomo sapiensmisc_feature1246, 2572, 2604n = A,T,C or G 2ggctaatatt ttttattttt atttttattt atttatttat ttttgagatg gagtctcgct 60ctgtcaccag gctggagtgc agtggtgcaa tctcggctca ctgcaacctc tgcctcccaa 120gtttaagtga ttctcttgcc tcagcctccc aagtagctgg gactataggc acgcgccacc 180acacccagcc aattttttgt acttttaata gagacgaggt ttcactatat tggctagggt 240ggtcttgaac tcctgacctc aggtgatctg cccgcctcag cctccaaaag tgctggaatt 300acaggcatga gccaccgcgc ctggccctga cagggctttg taatgctcaa ataatatgat 360ccaagggtca gagttgagta aactatagaa catagtcctt gcattccacc ccaggggccc 420aataacgagg tctttctcta aatctcagaa aacactaagc taagggaggt aagttctgca 480gatgggtttt ctctgtgtgt gtgtgtgtgt gtgtgtatac atatatatat acacacacac 540atatatatat atacacacat atatatacat ttaaaccttt ggtgctctac ttgtctgcta 600ccaaaattgt aggcagatga ctgaaaaatg taacacacag aatggcctgc atagaaacct 660ggctcttgga agtcatatga gtcagtttcc tcaactgtaa agtggtggct cactgtacag 720acactcagta agcacccagc aaatgaacac cttccagggg ttctgtattg gagtcagctg 780tatagaatga aaacaaatga caaaagggga aacacacaga gtttattatt ctcccccata 840aaaaaggccg gaggtgacag tccaagctga gatggcctct cccccaggca ctgtctctct 900actttgcccc cctgcggcac cttgttcagc tggcatgtca gcattccaca cagcacgaag 960gaggaaggac aaatggactc tccccatctc tctgcccact gcccccctcc ccacaacccg 1020tttaaaaaat agatatgggg ggccgggtgc ggtggctcgt gcctgtaacc ccagcacttt 1080gggaggccga ggcgggtgga tcacgaggtc aggagatcga gaccatcctg gctaacacgg 1140tgaaaccccg tctccactaa aaatacaaaa aattctccag gcatggtggt ggcaggcgcc 1200tgtagtctca gctactctgg aggctgaggc aggagaatag cgtganccgg gaggcgtagc 1260ttgcagcaag gcgagattgc gccactgcac tccaagccta ggcgacagag cgagactcca 1320tctcaaaaaa aaaaaaaaaa aaaaaataga catgggggcc tggcgcggtg gctcacgccc 1380gtaatcctag cactttggga gtccgaggtg ggcggatcac gaggtcaggg gttcgagacc 1440agcctggtca gcgtggtgaa aatccatctc tactaaaaat ataaaaatca gctgggcgtg 1500gtggcgcatg gctgtagtcc caaccactca ggaggctgag gcaggagaat ctcttgaacc 1560cgggaggcgg agcttgcagc gagccgagat cgtgccacca cactccagcc tgggcgacag 1620aggcgagact ccgtctcaaa aaaaaaaaaa aaatagacat agggtcttgc tatgttgccc 1680aggctggtct taaactcctt ccttgagtga ttctcccacc ttggcttccc aaagtgctgg 1740gatttacagg agtgagacac agtgcccagc ctctctacat tttttttttt tttttttaag 1800acagactctc actcagtccc tcaggctgga gtgcagtggt gtgatctcag ctcactgcaa 1860tctccacctc ccaggttcaa ttgattctcc tgcctcagcc tcccaagtag ctgggactac 1920acgcctggct aatttttgta tttttagtag agacagggtt ttgtcatgtt ggccaggctg 1980atctcaaact cctgacctca aatcatccac ccgccttggc ctcccaaagt cctgggatta 2040caggcatgag ccattgtgcc caaccctccc tgccctgttt taaggagggt cctgttcaac 2100aattactcta cagaccaatt gcccgcaact atatacttgg cagtgaggaa ctgctgggga 2160ggctgggaga ttcagcctat aggcaccttg gtggccctaa taactctttt atcttcttct 2220tcttccccat cagaacctgt tctcaaggaa aagagctaag gtaggctgcc agataaaatg 2280ctgaggtaag tctgttccag aaaatattgg gacatagact aaaaaattgt tgtttgttta 2340tctgaaactc aaatttaact gagtgtcttc ttttccccct aaatctggca atcctaatcc 2400aaggctaaac tcattttcag gacgcaaaag gctctggcct taccttttga gtcaggacgt 2460tgcactttga cagaaggctc tggaaggaaa ctttaaaggg agccttccag agggaaatgc 2520ggtgttgggg taggtctgcc tttggctatg ggctttctgg ctgccggagg gncccagggt 2580cccccaggaa agccttctgt gganggtctt ttgagagaga caaagcagag gggtggagga 2640agggcggctc aggtggaagg agtgaggaca aaggtgagtg cccctgggca ggaagtgctg 2700aaagagagaa ggagggaggc caccaggcct gggcctggag ccagcctggg agactcccag 2760ccgcccactt ctcggggcct cccttttcca gccccttgct ttcgaggcag cagtgccatt 2820atttggggaa accagctaac cagataggac agcaaaccgg ggatttatgt ggtgtgggaa 2880cagctcaggt ttccctccct gtttacccag cagtattttt taaaacagaa atcagcgtgt 2940gggtaaccgc agctgtgagt tactagctct ggctgtgagg gctggggtgg ggggagtctc 3000ttcagagccc tctgtccact ggcctgggag ctactgaagg aatgtgcctc tccccatccc 3060aggccaggtg gagaaggtgg ccctgcggaa gttcccagat cactgcccac ctcacccttc 3120ccctcccgac gaaggccagc acacctgggg gaggtgtgat gatggttcaa ggtctaaagc 3180tttagagatc agtcagttta ggggtcagaa cccatggagc caggcaagta aacacaggtt 3240ccccaagcca gctgggaggg agacacctgg gtgcctttga tgggagaaag agggggccaa 3300cagctacttg gcactggcca attttccctt gcatgaacat gggcccagtg taaccaacca 3360tatctttcca tttgtcaaaa aaaagccata tttccagatt ttttataggc aacctgtcaa 3420cttttaaatg ttggcaatga attcaaaaca ctgccattca attaaaagca gcccagaaca 3480tggcacgact ggttcttgga ataccaatct gcaatctctg atcttgtcca actctctctg 3540ctccccattt tacagatcag gaagctgagg cccagagagg cctagggact tagccatggc 3600tgcagcagtt gttattggga atgccatgag gccaggatgc ctgttatgtg ttctttgccc 3660agcctagcag tttggctggc ttggctatgc cagaggtctc accatgcagt tctcaagtgc 3720ttcctgagca tctctcattt gcatagacat gacatttaca aacacatcca tcatctactt 3780gattctggca atgacaccga gggagcagaa ctggcattaa tccatttttt ttaatggtga 3840gaacactgag gtttggagag gctggtgacc tgccaaagtc acagtcctag aaagggctgc 3900agccagggcc tccaattaca gatcaagggt ttttccaccc ccaaactcag tcatctcgta 3960aaaacgtcag gctccttggg gaatacagct tgagatggat aatcaactcc tcctaaaagg 4020gaaccataaa tggatgagat acttgggtaa actgaatatt ctttgtaact taaagttaat 4080tgaaaatcca taggttatca attttccaag caggagaata aaatagaagg tatttcaaag 4140agcaattata gtaggcatgg cttaatcttt ctttgatgat tctgaaggca tttcagggga 4200atgtaatgcc ttagggcatc attatgaaca ccaataatta ctgtgtaggg ctgtgacgca 4260aattctcatc aatcccctcc tggctgtcgg ggtgttttca gagaggatca tgcagggagt 4320gtgaacgtgt gcctgtctgt ttcttgctgc tctctccgcc tttgtcaatt tcagggtgct 4380gttgtggaaa atgcagtgtc cctggaaggg ggaggtcact taaccttaat gagcttctgt 4440ttctttatta aaatggggta atgcagactc ctatactcac tgtcaaggtt ttgtggggct 4500cagatgtcag tgtcagagca ggaagaaact ttagatcatg atatgcacca gcttcattct 4560gccatggaaa aatctgaggc ccagagaggt taggagcctc gtccaaggtc acccagccag 4620ggagagggtg ctagaacctg ggttttctgc ttcctaacct ctttcctgaa tataacacta 4680tggaagaaaa agatctggag gaacggaaag atgaacacat gattacacat gcaaacaata 4740ctggggtctc tctgatcaga gaaggtgtga tcttccagcc tcaggagagg ggccatccaa 4800gtcctggagg aggtgaaccc cttagttcag ggctgggagt agcctggggg cagcagagac 4860actgctgtga ggtttatagt ttcatgactg tcagagcttt ttaaaatgtg gtaattttaa 4920gtgtgcagcc tcccagggtc tttcttcttt taattgaaga aataaaccat ctcccctaag 4980gcatgcttgg cgaaggagaa aggcaggtgc aaggctcaca gaggagagca gcagcctaga 5040agggctctgt gtcatgggga agtaaaacat cccagaaaca gagagcagaa ggccttgact 5100gagccccagg agaggcagga caccaggggt gcacacccat aaacacacac atacacatgt 5160atgtctcctc cctggagcct gagagtccct atatacagca ggtgcatgtg ggccacacat 5220cacacaaaat tgaatacagg caggctcaga gcaccagcac acacgtatgt ccttgacacc 5280cttagagata ctactaagca cgtgtgtgta cctgctcacc catatggcag agcccctgga 5340tctgggcaga aatgccaaag caggggcagg cgcgtgtgcg cgcacacaca cacacacaca 5400cacacacaca cactagcaca gccacaaaag ctcaatccac atccagcatt cctaacaaca 5460cacacacagc tagacacgct aggagacaca tcaggacaat gtttccactc ccgctgccat 5520acacatatgc aggtccacat tcaccagtgg ggtagggtag agtctcatca gtccagactc 5580gcagacagct ggacacagag gtgatctctg aaacccaatg tctacacact gtggtctttg 5640ttacacacac acacacacac attgaaatga tgtcctcagc ctttggttat ttttggtttc 5700ttctgagctg gagtttcact cttgttgcct aggctagagt gcagtggcgt gatctcagct 5760cactgcaacc tccacctccc gggttcaagt gattctcctg cctcagcctc ccaagtggtt 5820gggactacag gcgcccgcca ccacacccta ctaatttttg tatttttagt ggaaacaggg 5880tttcaccatg ttggccaggc tggccttgaa ctcctgacct cagatgatcc ttccgccttg 5940gcctcccaaa gtgctgggat tacaagcttg agccaccgca cccggccgag ccttcgggta 6000ttttgaaagc tgaatgtgtg gttacatttt cttttctttt tcttcttttt tttttgagac 6060ggagtctctc tctgtcacca ggctggagtg cagtggcgcg atcctggctc actgcaacct 6120ctgactcccc ggttcaagtg attctcctgt ctcagcctcc tgagtagcta ggattacagg 6180catgcaccac cacgtccagc taatttttgt atttttagta gagccagggt ttcaccatgt 6240tggccaggat ggtctccatc tcctcaaatc gtcatccgcc cgccttggcc tcccgaagtg 6300ctgggattac aggcgtgagc cacagtgccc ggcctacgtt ttcaaacagc aatagcattc 6360gcctcctctg tcagtttaac ccccatcaca actcccactt ttggcaccta aacagttaat 6420ttcccagttc atgggccttc aaagtcctgc tctagtctct ggaggaactt tcacctacag 6480aggaaggtgt aagggaaact agttcatgga tttaagtaga aacattttag gtgtagcttt 6540cacatacaga ggagtgagaa aaaactgatt catggattta gatggaaaca ttgtagtatg 6600aacccagcgg agggtctggg agcgccttct ggtggtgaga attagaaccg cagcactttc 6660tgcaatgtgc ccaggccaga aagctctacc ttctgatagg acccacttct gaccctagaa 6720tgggggaact gatggaggtg tcaagccact gtggtcccac agctgcatgc aggcacaggg 6780gataggaaga gagctaccta caggttacta aaccattccc ttttaaaaca gcaccaggct 6840tatgtctact ctgcgcttcc attttctagg ttttaagtgg aagatatgtg aacacccagt 6900gggctggatg gctgtccctg ctacaagtct gtgatgtctc cgtccagtgg cagagctgga 6960aggcaggtgc tgtcggggct gcatctgcct tgttcaccag cataggccta aaaccatgga 7020gggggtgctt tggcttagat ccccacttgg cctgtgtgtg taagaggctc tcaggcacct 7080taatgctaca tcaccaacca aacctcctga tgattctttt aggttctccg tttccaggca 7140gattcacttc tgtagattta tttatttatt tttgagacag ggtctggctc tgtcccgcag 7200gctggagtac agtgatgcaa tctcggctca ctgtagcctt gacctcaact tcaagtgatc 7260ctccaacctc agcctcccag tatgagggac cacaggtgtg caccatcaca cctggctaat 7320ttttgtactt tttgtagaga cggggtctca ccatgttgcc cagctggtct caaactccta 7380ggctcaagcg atccaccaac cttggcctct gaaatgctgg gattccaggt gtgaagcacc 7440gcgcccagtc cctgacttct gtagacgttt gtattgttta catctactgt gtgcaatgta 7500cgagatgcag tcaggtgtct ggatggacca ggggatctgg catcttatag acttgggttt 7560gaaccccaac tgagccattt actggctggg tgactttggg tgagttctta aacctctctg 7620acccttaagg tggtaacagt atcaccagtg aagttggtgc acacagcagg gcccaaactg 7680acatctcaca gcacccagca ggtcactgtg ggcatgatag gatgatgggt cactgtgcca 7740gccctgaagg agttcaagtc cagatagggg aaggtggtgg accagaccca gacagagatt 7800ctgagtcgct gctgagactg ggtgagggta gtgggtacat gggaggacat atagcccggc 7860agcccagggc tggagtccac actcaggttg gggcagcctg gtctgcctct cctgcaggag 7920acttttccag gcaggcttgt ccctccagaa tgcacgaatc aaatcctctc aggatcagtc 7980tcattttcct cgtgctgggg gagcaggcta ctcacagaag atgttgttgc aaatgtaaga 8040atcacatgtc gatccacaaa ctggcattga gcagctacct aggagatcaa agaaactctt 8100actttgggag ctcctgccag ggctctttgg gaggtctggc tagctctgga ggaagagaat 8160gaacttgggg agggcgtgga acagatgagg acgcaggcac tgccattcaa agaggagagg 8220tctcccggac agggctggcc tgggcaggcc cagggagggt ggggctggag cagggacttg 8280aaaaagggag agggctcagg agactcagag gaggaggaaa gtgtgtgagc agtaggcagg 8340gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgcac gcgcgcgcat gcaggcctgt 8400gtagggctgg gaaagaacaa agcaaaaggg tgcacaaggc atccagaagc cagggcaatg 8460cagaacagga acaaagcagt ttggctcaag gggaaagttc tgatggaaag caaggaaaga 8520gaatgttaga agggctgaga gccagaatgc ccagtatggg gtacagaggg caacagaggg 8580gctgatgttg gtggcgaggt ggggaggagc atttcacagg gaactgggca gctccaagga 8640tgggctggag gagggacggt cctaaagggg gtggtgggag acaggcagtt agtgatgggg 8700ggagagaaat gctctgaggg gggctgctgt aggcagggag gggcaggtag caggaggcag 8760agggttgagc ctgcagaagc ccggagcagt ctgtagcttt ctaatgcttc ctagggaatt 8820ctgctgctga gtagccatgg gttcaacagg tctctaggat ttgccttgaa ttactgccct 8880gaggccccct aagtctgggg tctcataaat tcagcgggga gggcacacct gtgtctgagt 8940ctgagcagcc cagagccccc agtgttgtca gtgaacagct cactgaactt gcagagcaat 9000taaatcccag ggcagaagaa agagcaggag ggcagggtgg cccccagctc cctcccatcc 9060cgggtttggg gagagacacc tctggcctga gactcctcgt ggcctctaac aagtcttgct 9120ctctctctct tttttttttt tttttaaatt attgtctccc gccctccttc cttcttcttg 9180ggaatcagaa aagaaacctc aatgcctggc tttgcccctc cctggctgtg gtcaagccat 9240gtgacattta gacatttcag agcctcctga ctcctgaggc tggagacagc atgaagccaa 9300atgggcttgg caaacagaag agaggggcag ttaacattct ctgcaatgct ctcttacttc 9360ccgtttaaac acccctccta ctcggacaca gacccccaac ccctgtgcca ttgtgctcac 9420tcccaccttg gaggggtcag gctgtggtga aaggtcaagg tgaaaagtca tgatgatcct 9480attgctgcca cctccctggg gtgagcaaga ttctgtggtg gctccagctc tcccatggca 9540gtccacacag aggctgttcc aggttctgga gaggtcactt ggtgtgacct gggaatctgc 9600atcttttctc tgccaaagca gagggcattg cagcaaccac acctgggtcc cagtcctcaa 9660caaagtccct ccagctcccg ctgctgtctg caaggtttgg tcatctgtcc ctgcattgga 9720gaagagtttg cactgtgaat cttgcctcca ccagggaagg ctggtaaaag tccctgcccc 9780gggggccctc attgctccag ggtgcatctg ggggccagac ctgtggaccc tcacccttga 9840gcctcatttt gttcctctta gaacaaagcc atttctggta aataggtttg atgggtttgg 9900cagcagggaa ggcacagaac ctttcacgat tagcaggctc atgacaattt ctctttaggc 9960aagagagaag gttgaggagg aaagactggc agggcttgga gagctggaaa ggaagaaagg 10020ccctacaggc ctggggatcc catctctgac caccgacccc agcccccaac tctcatagac 10080tctgatttga tattcttatt taccaaagaa gctgcatgta tttgcatatc aattctcttt 10140cccatatcga cacgcaggga ggcttcttcg ttttggatca accagagatc accaatgtct 10200gcagggtgct cagcccctgg taccttggtc actctaggca gcctcctaga tgtgcctctt 10260aagataggtt ttctgaaatg ggagaggacc acgccccgcc ccccaccatg gactctggag 10320tctgcggcgc acaaatcctg cgattttaca gatggggaaa cagaggcacg gagaaagtct 10380cagctttccc acggcccact gggaccagaa cccaggtcgc aggacgggga cagccacctc 10440ctttttctct ccttgcaacc agcctgagct cgctcagcgg gtgggggctg ccgctatcca 10500gagcaccctg tttctggatg caaaataaag gcccaggcag tgtttggccc tcctctgccc 10560acagactttg gctccagggc agtctccgag agagaagcct tgggtcccac tggtccgagc 10620tctgcgtgct gagtctagag gctgcagtct ctaagccgaa actagcccag gcctcacagc 10680cgccttcccg ccggtctccc actgccttgg cggggcgctg gcgccctctg ctggccaagt 10740ctcgctctgc gcgcaaacgc cccagaaatc agccggaagt tccctggggc cccacaggag 10800tgaggaccca cctggattca ctttgtcatt tttttttagc tgcatgacct taagaaagct 10860aatgaacctc agagcactac ctgtgaagtg gaaataatag cgcctacact tcagggtcgg 10920tgtaaggatt gcatagaata ccgtatagaa agcacccagc acattgtagg tatgtttaat 10980ggtagcgatt attaataatg cccaactcac atggtgagat gagatctgag aagcctcctt 11040ataaaggtaa atgataacca agtcttgttg caaggcagct ggtgtgttac taatacatca 11100tgaggtggtg ggccctctgg ggactgtcag gagacagcct ctgttttatt aggaaataaa 11160acagaatctg gtctgctcca gttttcttcc ttttatttac tgcattagaa aactttcatt 11220ttatttattg cctattctgg gggttgaggg tgaggggaag gcatgactcc agccaggtag 11280gatacaaaag tatctcaccc cccaggaggg gtttgaggac cggccctccg gcttttagag 11340aagggcagct ggcttctgtt gctaacgatt tggatttggc ttttaacaga atatcaaggt 11400gaaagtgaaa ggagcttttc actccttgga gagtcaagag ggtgtggggt gggacctcca 11460ggggaatgaa gagtgagcga gggtgctggg gggatcccag acctaggaat cagatctggg 11520gaaggggtct gcaagacccc tccatgagtc aagaaaagcc ggtgggtgga tggtgagaag 11580gaccagtgaa gacactgttt gctggagttg tcccagccag tggctatgac tgagactgtc 11640ccatggcgtg tgcccagggt cctgccattg gatgatggga cattcttttt tttttttttt 11700tttttgagac ggagtctcgc tctgtcatcc agagctggag tgcagtggcg caatatcaaa 11760gtcgctgcag cctccacctc ccgggttcaa gcaattctcc tgcctcagcc tcccaagtag 11820ctgggattac aggcgtgggc caccacatct ggctaatttt tgtattttta gtagagatgg 11880agtttctact ccatcaggct ggtctccaac ttctggcctc aagtgatctg ctgcctcggc 11940ctcccaaagt gctgggatta cagacgtgag tcactgtgcc tggcctggat ggaacattct 12000gctctttctc catctgctgc ctacccatga tgtcttggga ctcctagaac cctaaaggaa 12060gcccctggac acgcaggaag gtgtggagag gagttctcat acttgcactt gggaggaggg 12120ctcaggagaa acagaggctg gcaacacccc ctcacacact ggtcctctgg agggccagtg 12180tctacagaca ctgtggactg agtccacaga gaggaaaggg tcctgccttc atcagaactg 12240ctcagcaagc agttccatcc cagggggtcc tgcgaggtaa gggaggggca gctagctagg 12300tgaggggctg agagagtggg gaggggaaag agggaagaag agagtgagag ggagagggag 12360ggactgagcc gattctcagc tccttgaccg tttgctgagc tctgtctgag tggacagatg 12420gtcccaagtc aggccacacc agagtggcct ttctgctccc ctacaccctg cattcctcaa 12480cattgctggc cccggagaga ctttccttca gagaagcaaa tggctgggga atggtgaaag 12540acactgcaga gaaaagaaag cacagcctgc tgccctggga attaacatga tttaggagac 12600ctgcaggtca ccccctcatg actaaaagcc atcctggaat gaaggtctgt ggctatttct 12660aggcaaaact gtctgataag ataaaatagc tcaactcctg accattaagt cgtgaaggcc 12720atggccatcg taaatctcat ctttccggcc ctctggcctg catgcagtgc agcccagcca 12780gtcggtggca gccaccttgg taggaagggc cctcatcctc ctggctgtgc cccaaggact 12840gggcaggctt cggtgccaag ggtagtgcga gcacttgaaa gccgccctgt atgtttattg 12900ttttccccag gtgatccaga attactcccg aactctacca gctgaaatcc tcctcaactc 12960acatcagaca agacggccct gccacttacc tgtcagatca ctttgggcag gtaagctcat 13020tttcctgaat ctttacttcc acaccttaaa atgtgagcaa tactatctcc ctggcaaggt 13080tgtttgtgag ggtaaaatga aacaataatc acgggtgcat cctggagctc tttcttacaa

13140ggcgtgcccc caaatctgtc ccctctttct gaggatgccc ttcccctatt gtctccctgg 13200ccatttccta cccattctca agggccatga tctcagggag ttctcctgac tcacccaggc 13260atattggatc tcctacgtac tgctacgact gcacacaggt gcaggaaatg gctgtttgct 13320ttgcgtttga ggaacttgga aagggagacg tggtagggaa agtagtggtt aggggcactt 13380tcagactgaa ggatgtgggt tggggaatac gggattcttg gagttgagga cgccgcttct 13440ctcgtctcta gctaatgtga gaaagaccct tctggacact aagcctgcaa ttccactggt 13500ggctaccagg tgtccgtggt gtcctggggc gggtgtaatg agagcgggag cctgtgaaac 13560caaaagcatt gtttttataa attcagcact cttcaatccc tattaataag gttagcggtg 13620cagttcttgc gtctcctgcc ctgcctcacc ttgcaatcat attcattggc attcctttct 13680tccaagaacc cacctaggag gccttgcagg agatatctct ggtgctggct gttcctgcag 13740tctgaaaagc cagttaagat acaaatatgt gagaggacac tgcttgaatc tgatttcatt 13800ttttaaaaag tttaattatt cacagacttt gcttctttct gactagtaat gtgacacctg 13860tgactcagtt caagatggtg tgtggtgacc ttgcagttga gaagcactga gcgctatagc 13920catgccaaga aggttgccct cctgggacca gcaaaaattc catccagggg gccatctctg 13980tcaggcttgt cctgactatc tttttggaga ggcggcagag tggagtggtt aaaagcatga 14040ttctggagcc cagctacctg ggagcaaagc tcgtctctac cgcttaccag ctctgagagc 14100ttaggcaagt gacctattct ctctgtgcct ctgagttttc atctgtgaaa tgggagtaac 14160aatagtcctg tctcacaggg ttgctgggag gcttgaatga gttaatgtcc atggggtgct 14220gaatcagtgc ctggcatacg gtgaaggcca tgtgagcagt aaatattatt attattagaa 14280aggttggctg ggcgtggtgg ctcacacctg taatcccagc actttgggag gctgaggtgg 14340gcagattacg aggtcaagag ttagagacca gcctggccaa catggtgaaa ccccatctct 14400actaaaaata caaaagacat tagccggtat agtggtgcgt gcttgtagtc ccagctactc 14460aggaggctaa ggcagaagga tcgcttgaac ccgggaggca gaggtttcag tgagctgaga 14520tcacgccact gcactccagc ctgggcaaca gagtgagact ccatctcaaa aaaaaaaaaa 14580aaaagaaaga aagaaagaaa aaaagaaaag gtcccaacct actcacttat tgttcacttg 14640catcaaatgc taggttcagt gtattgttct tgtaagatta gaaatggaga gatgggtcag 14700aaggggttgg tcaaaataga cctctcaacc caagcagaca gggctggctt catggccggg 14760caaccatgca ggacccaggg ctctaagata agaaaggcac gtagtttaat gctttgctgt 14820cccatcttga tactgttttt tttttctttt ttttttggaa atggagtttc actattgtca 14880cccaagataa agtacaatgg cgtgatctca gctcactgca atctctgcct cctgggttca 14940agcaattctc ctgcttcagc tccttgagta gctgggatta caggcatgta ccaccacact 15000cagctaattt ttgtattttt agtagagacg gggtttcacc atgttggcca ggctggctgg 15060tctcgaactc ctgatcgcag gtgatccacc cacctcggcc ccacaaagtg ctgggattac 15120aggcatgaac caccgtgcct agctgatatt cttaatttat gaaggaaggg ccccaaattt 15180tcattttgca ctgggcccac aaataacgta gcaggtccca caaacaaatt cgtctagatt 15240cagagggccc cttgccttcc tcctctgctc acattcgttc ctttctccca tcacaggcgg 15300gtaccctacc ttgggggatt tgccccagaa taagcctttt ttttcccttc taacatttta 15360atgaaaaatt tcaagtgtat agcaatgttg aaaagatttt atagtgagca cccatacacc 15420tgccacccaa agtctaccat taatgtcttc tggacttact tttgccgtct tattcatact 15480ctgcctggac tgtcctcagc tacaggactc acatctcttg ccgacagctc taaggcttcc 15540agtcctgctg tctggaccaa gaaaggcttc ctgggctctg agtgtcaaat ggcggccttc 15600aaggaagggg aatggtggaa aaggccgtgg ggggttttgg agaaattgct agggaaagac 15660tggcaccaga gttccaccag cccaggcaat ggggggtaca gaacccataa gatgagttct 15720agaaaagcaa ggaaggtttc gttctggagt ttgtggactg aggtttccat ttgtgactag 15780gattctcatt ggttccttat gtagtttctt acgccctgca tagtcttcta agcatttctc 15840acagacgtgg tctgggggcg aacaccagca gccctgagag gtggctaaag aggagattct 15900tctctccact tcacatcgta ggaaactgag tctcagaggt tccttccctg gcctgcccac 15960aaccccaggg ctaaaagagg cagaccaagc ccagggcctt gaaccccaac aatgggcctc 16020tttcttttga tcccatgata ggggtgcaaa agcattgcat tcccctgggt aatttgaaga 16080aaaaacccaa aaaactccaa ctttgtctcc aggaaaaaga gggtgtctgg gctatgattt 16140acctctgagg gtgtggttgc actgagcgtg atcacacttc aaagggttag atctcatttc 16200tctgcctttc tagcttgggc ccagggctca gaaatgtgtg gactccctca cagcccctcc 16260cagcatccct gccccctccc aactgccttg ggcaggtgac acctgtatta ttgctaaggg 16320ttaaaaagcc cccaaatcaa taaaacccat taatgagtgt tggtacctcg aaggctacag 16380ataaatccct tctactcagt gagttcaatc ccataaaaca gctctcccct ttcaatccta 16440gcattcattt gatagaaaat gtggagaaat tttaaaaagg tgacttacta attgcctgta 16500aaataaaagg cagatggaag ctttattaca gttgaaggaa gtcgggaata ttaaggtaaa 16560atgtcaaata acaattgatt ttccttagac ataaaggggc gatttatggc ttcctagtta 16620ctacaaacga gaaattattt gaagttctga aaagtatgag gagaaataaa gattaaatag 16680aagatgaaat catagggatt tctctgggag gtgacttcag tgcccctggg gactagaatt 16740catgtggcca gtggcctagc cagctggggc ttggcagttt caagatttag aggcaaggtg 16800tctctgagga gcggggaagt ggctgtttgc tttgtgtctg aggaactagg aaagaaagat 16860gaagataggg aaagttgtgg ttagggccaa tttcagactg agggatgtgg gattagggga 16920ttcttgtgga tgggcctata gctctgcttc ctgactagca gatactgggg atctggggaa 16980gggaagggtg agctgccttt cctgggactt cgatggcatc cttacagtca ggggacactg 17040tccttgctgg gtcccggaca tcagtgtcta tgtatccctg caagccacag cactatccag 17100gccctctggt ggctttgctt ggtctgggcc aacctggtct ccacactgac agtcaaagac 17160gaggagagga aaaagaactc acggctaggc gtggtggctc ctgcctgtaa ttccagcact 17220ttgagaggcc gaggcgggtg gatcccttga gcccaggagt ttgagaccag cctgggcaac 17280atggcgaaat ccccgtctct acaaaaaata gagaacaatt acccaggaat ggtggtactc 17340tggaggttga ggtgggaaga tggcttgagc tcgggaggtt gaggctacag tgagctgtga 17400ttgtgccacc gcactccagc atgggcgaca gagcaagaca ctgtctcaaa aaaaaggctc 17460atttccaggc tgccaggctt atgttagcct ggggtcccgc aggagctgac ctgagacaag 17520tacttgaggg caagttgttt gtttgaggga tgctcacagg aagctccagt aggagggtag 17580ggaggtgacc cagggaagga aggcagctta caggggcgtg ttgtcaggaa gggcaccccg 17640gcgggtgacg gaagcttaat tttgctggga aactcaggag ccagcatgga acctgcacct 17700cagagttatc ccactggaag gatgagggag cgggtactta tacaccaact ccatccccgt 17760cctttgttaa ggctgctgga tggagtgggt gggacactca ttcttcagca ctcccagggg 17820ccctcaggct gtcagaagtt aggtccgttg ggctccagga tgaggggacc cccagaagat 17880gcgggagggc gtccgcagca tctgcctctg tgctttccct gtttatctga atcctcataa 17940ttcccgccac atgcagatca gagcccccag ctttatggaa gagaacacag gtttggagaa 18000gataaagagc attcccacaa ctaggaggtg gggaaagcta ggagttcagc ccagagctcc 18060ctgacttcaa agtccattct ctttctactt cctgattttt tttttttttt tttttttttg 18120agacggagtt tcactctgtt gtctaggctg gggtgcagtg gcacaaactt ggctcactgc 18180agtctctgcc tcctgggttc aagcaattct cctgcctcag cctctcgagt agctgggact 18240acaggcgccc gctaccatgc ctggctaatt ttttgtgttt ttaatagaga cagggtttca 18300ccgtgttggc caggctggtc tcgaactcct gacctcaggt gatccgcttg ccttggcctc 18360ccaaagtgct gggattacaa gcacgagcca ctgtgctcgg cctacttcct gatttttgta 18420taagacacat cccagcagca tggtagactg aagactcctg ggcccactct cagagattct 18480gctctggcaa ggatgtgttt attggtgaga ggggtgtcca ggaatacgtg cctttttttt 18540tttttctaga gacagggtct cgttgtccag gctggagtgc agtggtgcaa tcatagctta 18600ctgcaatctc aaacttctgg gttcgagcga tcctcctatc tcagcctcct aagtagctgg 18660gactacaggt gcacgccacc atgtctggct aatttttaaa ttttttctgg agttggggtc 18720ttgtgatgtt gcccaggctg atctttaact tcaggtctca agagatcctc ccaccttggc 18780ctcccaaatt tttgggatta cagccatgag ccactatgcc caactagaat atgcactttt 18840ttttttttga gaccgagttt tgctcttgtt gcccaggctg gagtataatg gtgcgatctc 18900ggctcaccgc aacctccccc tcccaggtcc aagcgattct tctgcctcag cctcctgagg 18960agctgggatt acaggcatgt gccaccatac caggctaatt ttgtattttt agtagagatg 19020gggtttcttc gtgttggtca ggctggtctt gaactcctga cctcaggtga tcagccccgc 19080ctgctttggc ctcccaaagt gctaggatta caggcatgag ccaccaagcc tgaccttttt 19140tttttttttt gagacagagt cttgctttgt cacccaggct ggagtgcagt ggcacgatct 19200cggctcactg caagctctgc ctcccaggtt catgccattc tcctgcctca ggctcccaag 19260tagctgggac tacaggcact tgccaccacg cccggctaat tttttgtatt tttagtagag 19320atggggtttc accgtgttag ccaggatggt cttgatttcc tgacctcgtg atccacctgc 19380cttggcctcc caaagtgctg ggattatagg cgtgagccac cgtgcccggc cttttttttt 19440tttttttttt tttttgagac aggatcttgc ttggttgccc aggctggagt gcagtggcct 19500gattacagct cactgcagcc tcaatctcct ggactcaagc aatcctctca cctcagcctc 19560ttgagtagct gggaccacag gtgtgtgcta ccacacccag ataatttttg tgctttttgt 19620agagacaggg tttcgacatg ttgcccaggc cggactaaaa ttcctgggct caagtgatcc 19680tcctgcctca gcttcccaaa gtgctgggat tacaggtatg tgccatcgtc accagccaga 19740atatgcattt tctttttttc agacggggtc tcactctgtt gcccaggctg gagtgcagtg 19800gtgtgatctc agctcactgc aacctctgcc ttctgagttc aagcgattct cctgcctcag 19860cctcccaagc agctaggatt acaggtgtct gccacaacac ccggctaatt tttgcatttt 19920tagtaaagat ggggtttcac catgttggtc aggctgatca caaactcccg actgcaagtg 19980atccgcttac ctcggcctcc caaagtgtta ggattacagg catgagccat tgtgcctggc 20040cgaatatgca cttttaataa gcatcagcca ggctaggcag gccgggggcc acactcgaga 20100acatttgcac cacagccact ggctacctgc ccctttttcc ataaggttcc actgccctct 20160ctcccctcta tctgggtctg ttcttcaggt tcttccctgg gagctctctg agtgacataa 20220ctgtccccaa gtgctgggag atggagagag gaatcaccag actggagcag gcccccagag 20280cggagatggg aaggggaggc tggtgttctg aggctcccga ggcagtgaga ggtgaccgga 20340ggcagtgaga ggtgaccgaa gacagtggct gagaaccagg gaggggctgc gggaaaaagc 20400cctgggtgca agtcgctctt tccttagcgt ctttgagagg agggatgggg aaaggtgagg 20460tactagggaa aaccatctgg aaggaggtca ggctgcagaa aagctgcagg agtctggggg 20520actaagaaaa cagtgggaga ccccgctgca gcccagcacg tgagggtgag aacgtcatga 20580atgagggaag agcaggcagg gggtggtggc cttgtggcct ctggagagga ggagccacat 20640gactctgggg tgatctgggg tgaccctcag ggtaagggta cctccctagc actgagtaca 20700gagggaagcc tacactgcca ggtgcagttt ccctggcaat gcttctcctt ctaacactat 20760gtgagtttcc tagtgctgct gtaacaaact gccataaacc aagtggcttc agagaacaca 20820gacggactat gacagttgtg gagggaagaa gtttgaaaag cggggtgccc gcaggctgca 20880gtgcttctgc aggctccagg gagaatctgg tccttgcctc ttccagcctc tagagcctgc 20940cgcactcctt ggctcatggc cccgtgtcat tgtagcctct gcttctgcca tcacatctcc 21000tcctctgcct ctcctgcctc cctctgtcct ttataagaac gcttttgagt atattggacc 21060catctggata gtccaggata aatccctcca tcctcatatc cttaatttaa tcacacctgg 21120aaggttccct ttgccacata aggtaacata ttcacattgg gacagttagg ggattgggat 21180gtggacatct tttgaggtgg gaagaaggag ctggggttct ttttaacatt tttttgagac 21240agggtctctg tcacctaggc tggagtgcag tggtgtgatt gagactcact gcagcagcct 21300caacctcctg ggctcaagtg atcctcccac ctcagcttcc tgagtagctg ggactacagg 21360tgtgcaccac catgcccaga tgatttttgt attattttaa tattttgtac acatggaggt 21420cttttaatgc tgcccaggct ggactcaaac tcctgggctc aagcaatcct cccgtcttag 21480cctcccaaag tgctgggatt acaggtgtgt gccaccagac ccagcccgga cctggggttc 21540ctgtctcatg tcagccttag caatctgggt gaccctgggc aaggcctatc ccctctctgg 21600gcctttgttt ctccacctgt gcaatgaggc tgttccctct ggctccttca ctctgagttt 21660ttcagttggg agaatatctt ggcagggagc agaggtcggc gggggtggtt gtcattccat 21720ttcagggcct ctcagagtcc tgccgtggtg tgcactgtgt gtgtgtttaa ttttctacat 21780ttggatgtga tcctaatcca ataaatgctt aggagacttc tatagaatag attaattttt 21840actagaaaaa aatataattg gctgatgtta aggctactgc cctgacaaat ctgccttggc 21900catatatctg agaaggtaaa agacccgcta cgcttgcaca taaatatgcc atcttcccca 21960caggccctgg agaagcaccc cggggaggtt tcccttggtg atttattctt cattaataag 22020ctctatgcta tattaggatc agatttatga ctctgccttt ctaatatttc tgacatttca 22080tctgaaaaga attacaaatg aaatcttgaa actttgccac ttctccctgc tagtgctctg 22140gcactctgtg tccaagggga gatggtgggc tggggagacc ccaagaagca gggacagagg 22200catgtttctc agggaaagga gcgatcagct tgactttggg agagctttat tcagtttgca 22260agcagcttgg gaggtgagcg gttcaggcga gaaggctgca gaccagacca caagcccagc 22320agcagcagtg atgcctgtaa catgtatgag atggtggcag gcacattcat tcattcaaca 22380gctatttgtt aagcacttta ctgtgtgcca aacactgtgc tgcttggtgc ttgggataca 22440ggaaagaata aaagtgaagc agtgatgaag atcttggcct tcatgcatat ggtgttctag 22500cagaggtagg ggagtgtcaa gcactgatga atgaaaatcc tgaataggta aaatatatca 22560tatgttcaca cgtggtaagg gctatggcaa aacagaaaca aaccctccac acagggaaaa 22620gggaaccaag agtgccgggt ggtcaagttg caatgttaat taagaaagca cttcaactca 22680tcaactcaag gaaccgtatt atccccattt tacagatgag gacggaggct cagcgtggat 22740taggagctac acaaagacac aaagggaact ttgcacaact tgaaagtttg caaagtgccg 22800tctgagccat tagtctccct tctccccact gactgcccta ccacaatcac atggggacca 22860taaaaatact gttgcctggg tcccgctccc ctgaggcttt gattcaattg gtttggatgc 22920agcctgggac ttggcatgtg actcttgtgg tgccaggact aagaatcttg tgtgaactcc 22980cagcacagcc tctcaggcct ctattttctc acctgcaaga tgtgggtaaa aatattcacc 23040ctgtactgca agaggatgtc aggagtgaag gcagaaacac agcaccagcc ctccaggacc 23100cccaacccct cccccacccc aatccttcac ccctgttgta ccttctgacc tcaaagtggc 23160tctgattatt tcactcccac aggccactgg ctcagaggta tagagctcac ctgtggcaga 23220tggagatgcg gatctgaggc ttctgatgct gccacaccca gcggcgcccc ccaaattccg 23280ggcccctgga tgacatctgg tctgttcctg cagcatcaga gcacaataga gccagccacc 23340agtcccagcc ctgcctgcat cccatccatt cctgggtgcc taaccccgag gatcccctgg 23400cagtatgatg cggacctgtc ttggatccca gggatatgct ggccacgggg aggagccgga 23460aaccaacctt tgtgtcactg tgtagtgaca agtgcctttg gaggtcacaa tagccagtgg 23520tgatttctac cactgccccc agcagccaag gtggcagagg agccctgtca gtcaccccca 23580ttctgttcat ggtctcacgg tgggctccac atggggggtg gcagccctct cccccacccc 23640acccgacccc tttcgacaga tagggtaata caaatacaaa taacaccaaa agattgagtt 23700gctgggcaga aagggaccaa aggccagtgt gtgtgtgagg ggtgggggca gggcaggaga 23760ggagcagcaa aaggctgtga ccgcctggct gagcactgga tactcactga agggcaggga 23820ggcttcctgg agaaggagac ctggcagggg ctgagggagt gatgccaggc atgggggttt 23880ggagggaccc caggcatggc atgcctccat tcctccctgt gctatccact ctatataagg 23940ggtgctgtgc agggagacag cttgcatcca agcagggagg cagggaggat gagaggcaga 24000gaggagccca gctgggttga tggaaagtct gggaaatgca ggaaatccag gagggggaga 24060atgattccaa gctgtggcct gtgatgggcc ttgaaaccag gtgtaggcac ttggatctga 24120tcgctgggga gccagagctg cttcctgagc agcagaaggg caggatgcga atcagactag 24180gggcagtggg aggaactgag aggcctcagg tcaccggaga aaatgcacag ggccgggagg 24240cagagatgct cctgttttct tgctctgggg ctcaggacag tcagtcaccc tgagcttcag 24300actcagctca ctcattttgc agagatcctg acggcgatgc ttcaggatga tctgggaaga 24360gtcaatgagg taaaatatgt gaaatatgcc ttgaaaacta caaaccacag cacatgttct 24420gtttttgctt ctgcttgatg gactcagtga gatggtgggg acaagaatta gagaagccca 24480tgaggaggcc aaggggcacc aaatagaccc accaaggacc agtggggact tagagaaagg 24540atgagtcaga gagaaatgac aggagcagaa ggcaggcctt gtatggagga tgaaggtgaa 24600gatcatacac cattaaactt gagaaagagg cgggaggagc tgccatttct tgagtctact 24660ggatgccagc agtagtgctg ggcaagggct taacaggtgg ggaaatcgag gcacagagag 24720gttaagtagc ttgcctaaga tcacccagtt agtaagtagc agagcctggc agcttaactc 24780caaagtcttt gtactaaagc cagattttcc aaatttgccc aactgtaaga atcacctggg 24840cctgtaatcc tagcattttg ggaggctgag gtgggtggat cacctgaggt caggagtttg 24900agaccagcct ggccaacatg gtgaaaccct gtctctacta aaaatacaaa caattagccg 24960ggcgtggtgg caggtgcctg taatcctgta atcccagcta cttgggaggc tgaggcagga 25020gaattgcttg aaccccaaag gtggaggttg cagtgagctg agattgcgcc atcgcactcc 25080agcctgggca acaagagaga aacgccatct caaaaaaata aaagcccggg agtttattac 25140agatgcatat tcccaggcac ctcctacgga ggttttgagt tagtgagtcc aaggccttct 25200gcctcttccc aatgtattca ttatgcacca tcattactct tgttcagata caatgtgagt 25260gatagcttgt ctctggcagc acagcagcca cacccaacca atccagacct cagtcatgag 25320ggtgccaatc acagctaaca tttttttttt tgagacggag tcttgctctg tcgcccaggc 25380tggagtgcag tggcacgatc ttggttcact gcagcctctg cctcctgggt tcaagcgatt 25440ctcctgcctc agcctcctaa gtagctggga ttacaggcac ctgccaccat gcccggctaa 25500ttgtattttt agtagagatg ggggtttcac tatgttggcc aggctggcct tgaactcctg 25560accttgtgag ccacctgcct cagcttccca aagtgctggg attacaggtg tgagccaccg 25620cacctggccc acatagctaa catttaatca gcacatacag ggccatgctc atcatttttt 25680gcgcacaatg ccatttaacc ttcacaacag cctgtgagaa ggtgtgttag cctcatttta 25740cagaggaaga aactaaggcc cagagaagtt atgcaacttg cccaaggaca cacagcttga 25800aggagctgag gtttaacccg tttctacggg gtctgaatcc tccttaccac ccctatctcc 25860cctgactccc aggttgtgtt tggtgtactt gggtagtgtc cagctgacaa atgagatgtt 25920ttagcttcag acagtctatg ccatgtagaa atgcacccag gactgtgcat attagggagg 25980tttgcaaatg tgtccacatt agataatttt tctcaaaatg cccctcactg aacttctgtc 26040atgcagtatg tactgagcac ctgcctcttg ctaggtcccg ggagagagga tgaagagggc 26100agagccccca ccctcagggg acctgaaaaa tgggagtcct ttgtgctcat ggaagcaggg 26160ccttggggcc tcagcactat ggacatttgg ggccgggtaa ttctttgttg tggggggctg 26220tcctgtagat tgcaggatgc tccgccgcat cccttgcccc tgccctctag aagccagtag 26280caccctccag ttgtgaaagc cgtaaaatgt ctccagacat tgctcccagt tgagagccac 26340ttcattaaag aaaaaaaaat aaaaaagatt tctgtatcct ctatacatgg actgaatttc 26400actgacattt ctctaaaata actgaatcta tttctatact catttttttt taccagctgc 26460cattttattt atttttcttt tctttctttt tttaagaaac agggtctcac tctgtcaccc 26520aggctggagt gcagtggcgc aattatggct cactgcagcc tcgacctcct gggctcaagc 26580aatcctccca ccttagcgtc cccagtagct ggcacgccac tgtgcccggc tatttatttt 26640attttatttt attttttttg tggagacagg gtctcccaat gttgttcagg ctggtctcaa 26700actcctgggt ttaagcaatt cttctgcctt catttcccag agtgttagga ttgcaggcgt 26760gagccactgc acccggccat tttatttctt aacaaagcac aaatcaaatg tacaatgcag 26820ctagattttc ctttataaat aatgtctaat ggatttgttt ctgtcagctg cctagaacat 26880tctggtatcc cagacagaag tgcaacacca ggtggaggtg ttgcagctga gaagttctga 26940ccagcatacc agcaccccct taaaagctgt ctatacttgc ggttctcagc taggctgcca 27000cagaatcgcc caagtcttaa aaaaacaggt ctctgtccta ctgcttgaga tgctggttct 27060ctaggtctga ggttctggca cctgtgtgtt tttaaaactt tacaaataat ttaaaagcgc 27120cttcaagtcc acaacaacag aatatttccc tccactcctc attgtcctgg agttctctaa 27180cagtgcagtt ctacaactgg acacacgatg tcgcttttca gccacagttc tcactaagcg 27240gccccacagg gcggcaggtg ccttctgcag agagagagag agggccttgg ctgacaggcc 27300aagaccgggc atcctggctc ctcctctgta cagacttttc acagatgtga actctcccta 27360ctccctgtct tctgccccca aatgaagcct ctcagctggc aagagctgag aactaccaag 27420cgagccattg ctaatttcta ttgtgtttgg aaccacaaaa ggcagaatta ttaaggctgt 27480aaaggacctc agagcatctg gtgcagtgag tttccaactt gtgaaaatct gatgtgatct 27540cggacaagtc acttaacccc ctgcctcaat ttcttcatct gtaaaatagg ataggaatat 27600atcttgcccg tctgattgtt atgaagacaa agagaacaaa tgcacataaa gcccgtgacc 27660atgtgctttg tgaatggaag ctttaattta ttcattcatt tattttatta tttattttta 27720tttatttatt tatttttgag acatagtctc gctctgttgc ccaggctgga gtgcaatggt 27780gcgacctcgg ctcactgcaa cctccatctc ccggattcaa acaattctcc tgcctcagcc 27840ttctgagtag ctgggattac aggtgcctgt caccatggcc agctagtttt tgtattttta 27900gtagagactg ggtttcacca tgttggccag ggtggtctca aactcctgac ctcgtgatct 27960gcccgctttg gcctcccaaa ggaagctttt atttttatta tagttttaca taaggataaa 28020ttcagcctta gtgaagggaa gtgacttgcc caagatcata cagtgagact gctggatctg 28080gggctctact tcagaatttt tttttgagat ggagtttcac tctgttgccc aggctggagt 28140gcagtggcac aatctcggct cactgcaacc tccacctccc gggttcaagc gattctcctg

28200cctcagcatc cctgatagct gggactacag gctcccacca ccacgcccag ctaattttta 28260tattttgagt agagacaggg tttcaccatg ttggccaggc tggtctcaac ctgcctggcc 28320tcccagagtg ctgagattac aggtgtgagc caccacgcca gtctactgcc attgtccatg 28380atttttccta cagggaaaat cacaatccca gaagataaga caaagaacag taaaaggtgg 28440cttttgaggc agtgagttct acctgaaggt gggaacagcc cagagtgtct ggggacagag 28500tggtaaattc taatcaagcc ttcccatggc tttgtggatg aggatgagtt tctaccctga 28560agctcggcct gttcagccat aaaatcagga taatggtggc tgtgcctcct tagagtagaa 28620tgagaatcag agcagaacaa gggaaagctg cagtgacttg tcaggtgtca accttcagca 28680tgatgggaga gccatgggac ccttcccctt tccctaagag agccagccct cacagcaggc 28740ctgggatcca atgcccagca cccagctggg agcccaggga ccttggccaa aatctggttc 28800tgcttcaact tggtgcccgg cctttgtcaa gtcacttcct catttgcaaa ctgggagagt 28860ttggatgaaa ctattgaatg aaattatttt gggggtttct ttctggctct cacagtcctc 28920gcatgctcac catgttccct tcaatttcat tagcacagcc caacaaaggg ttaagcagtg 28980gcagttcctc tcgttctttg gttaggacag gaaggtcagg ggtgaggcca atacaagagg 29040tagccgccac agctgatgct ggaaatgaca atagttcttt cctagactca tatttgtccc 29100ctctccctga agctttgcct gcagtgccct tgtaaagaag ttggcaagaa gcaggagtga 29160ggctcagccc ctctctgaaa tggatacgcc ggttgctccc cctcatggct ggtctcattt 29220gccttcttca tttttagaca cattccaaac ttttcagcaa attatagtgt ttgccaactg 29280gccgtctggg gcccaggaga gatgctattt atagcgatgc tgggatgctg ccatcccaga 29340gcagcctggt aagaaacgga gccagagtgc ctgggagtgg cgtcctgcac cctggggaga 29400ggccagggcc ctggagcagg gtggcaaagc tggtggcccg tggcaaggac cactggcaca 29460tcccctgcct gcctgggccc tggggtctgt gcccataccc cacacggggg gctgcttccg 29520tgctccttgg agagacgatg gtgctgtggg gccactgagc acagtaaagg ctaagaccca 29580ccataggtca gcccttgctc atgctgactg ttgccccatt tcccttcatt ctctcactcg 29640ttcattcctc agaatctgca tcctggtttt gtcactacct ggagttgtaa agataccacc 29700aagctcacct tgtggtgtga gccttgattt tccccatctg tgtaaagggt ggatctgagc 29760tccaaagttc cttctagtca tatgcagagt gcataataaa tgtgtttgta ttcccactgt 29820gctcagtggg cactggggca tgcagaagag aaattggaat aaatgtgacc cttgtcctcc 29880agaggctcag taccagactg gaaccaggac ccagatgagg ggcctaccca gagagggcag 29940cgtgctctcc tatttgtgtt aggcgttacc atttacaaag ggctttacgg ctttgaaggt 30000ccccacaacg ccctgaggag gtgaagtgtg gcaatgcccg ttcacttggg aaaatactgc 30060atctcagaga gaccaaggga ctcgcttaag gtcacacaga tacagtaagt aagtagggaa 30120agagctgggg cctctgggct ccttttccgg ggctctggcc tcggttttaa cccgttctcc 30180tagacctctc agtctctggg cctcccctct gtgttcccca cccccacttt ccatcaagag 30240ctcagttctc ttaagttcta tattctctct tccccacccc tagaaatctc tgcccgcttc 30300cagaaaaggc tttgattccg tcatttttgg catttcccac ccaggaatca aggctgcctc 30360ctctgtgagg aaggcgtgtg gaaggcgagc agctgaggac acctcttttt aagagaagct 30420tctccatgtt cttagccagg ttatataact tcttttgctt ctttcttttc cacattctac 30480atatttttca caatgaggat gtgtcacttc tatagttaaa aatggaaatc ttcatttaaa 30540agaacaatca gacacaaggc aaggtatgct taccttccca atgaccaagg agcagggaga 30600cattggtcgt ggaggccata ggtgaccagc cttgagggaa gggaaggaag gggaggtgca 30660gggaggcgca gcagaggcac ccgagtgtgc tctacgaatg taagtctgcc agctgctcct 30720ctgtgcctag caccctggaa agcgcccgca cacagtgggc cctcagcaaa ctcccactga 30780gcaaagggcc ctgtgagtaa ggacctagaa gcagggtgtg cttgaggcat ggggtgggag 30840ggcatccagc ttggctggcg gtgggagccg atgcaaaggt gagctgagac cagacggtag 30900aagaccttca gtgctgggcc gaggagggtt ccttcatccc atagagaaga gctgccatcc 30960aagacagaag gctggggagt gacatgttga aatcagtatc ttaggaaaat aaagccactg 31020gctgggccac tggctcccct ttgtagaggg gtctacgaag ctcccgaggt ttcagatagt 31080ccctgagagc ccttcccctc gcgatgggct ccgtttgagg catgtccagt gtgaaaggac 31140cacaggccac ctgagggaca ggaccaagca gagagctagt gacagaatgc ccagggctcc 31200aagacagggc tggagaggtg ggagtggtcc ccaccttgag agccaaaggg gctgagggct 31260gagggttgaa ggccgagagc caggaaggtc caggggagca aaagggggag cagaggggga 31320gctggaggtg ttggggtggt gggtagagag ccaagatagg gaggggacaa gagaggggag 31380aatctcaagg aaaagcagga gcatgagact gagagaaggc ccctggaaca ctggcttgaa 31440tgtggctgac attggcactg ccagtactca tcccaaacca gggagctcag ccagcgccct 31500ttctgcatgt ccgctctttc atcagacagc taggaacaag ttatacgaga tgatgtccaa 31560gtccaataaa tgtctaagtc ctttccactg gatctggccc cagcctcctc tctgaaccat 31620ctcctatcac tccaccctat ccctgctggc tccttctgct ccagccatac aggcttgctc 31680ctgggctcag cttgtgcctg cctggggagc ctcgcgcttg ctgtttcctc cttgagaaat 31740tcctgccctg gataactcct tcccccactt ccttcagatc tctgctccct tatcaggggg 31800cttcccctgg ctgccctatc tataacagcc ctgaccactc ttttcctctt ctcctgcttt 31860ctttgtcttc ctgttcttat cactaagaca tcacacatgt cttagttggc ttgtttatat 31920tctgtctcca cccatcttag tcagctactg ctgaaatgat gctgtgtaac aaaccacccc 31980gatactcaga ggctccaaac aagtgctgat ctttctcatt tgtgagtctg tgtgtcagcc 32040cgagcagggc agctccaggc tatggcttgg gtttgggtgg actccatgtc tctctccatt 32100ctccttggac cagcagctcc ctggggcaca tgcttgtctt ggagaatggc aggaacttaa 32160aagccaagcc aaacctcaca gcacggttaa gggtgatcat gacacaccgc taacattcta 32220gtggccaaag aagtctcatg gccaagtgta atagcagtgg attgggcaag tttactctct 32280tacacctgtt gtgaagggga gtgggagggg atgggggggt gaccgttttc acaacaacag 32340tgcaaactat cccatctccc aatagaacat aaacacctta aggttggaac tgggtctgta 32400tccccagcac ttaaaatagt ttttggcata cagtaagggt gcaatagaca tttgttaaat 32460aaatatacag actaaccaat tagcataata cagatgacaa aggtgtctct cttctgccag 32520cttctgtgcc tgaaatgttg atgatctcct ccctatcttc ctaacatctc tcctgtcccc 32580acctgtccat gcccagggtt tggctgctgt gacagaagtg tgagagcctc ctgtctccct 32640tgggatccca gtaagagctt ccatgcctct ctccctgctc acctgggctc ccatccctgg 32700ggaccttctg gaaacagctt ccagggctcc cagagcttac ttagccagat tctacatctg 32760gctccagctg ttatcctaag cttggccttg ttttctgatc tgaccacagc ttcatcactc 32820ctacctgact gtgggattct agcccccagt ggggtggggg accaagggtg acaattacct 32880gggaccttga ctattgaaag gctaatcagg tttgattggg aaaaaagagt tgctaaaaag 32940gattgtattg gataattggt gagatttgaa catggatttt atattagtat tgtgttatta 33000cattttctga tattagtcat tctttttttt tttttttttt gacggagttt gctctgtcac 33060caggctggag tgcaatagtg tgatctcggc tcactgcaac ctctgccacc cgggttcaag 33120cgattcccct gcttcggcct cctgagtagc tgggactaca ggcgggtgcc accacaccca 33180gcttgttttt gtacttttag tagagacagg gtttcaacat gttggccagg atggtctcga 33240tctcttgacc tcgtgatctg cccgcctcag cctcccaaag tgctgggatt acaggcatga 33300gtcaccgctc ccggccgtta gtcattctta taggtatgta agaaaatgtc cttgaggtat 33360ttagcaggga agttcatgat ctctgcagct tactcacaaa tggttaaaca gaatgagact 33420aagaacagac tagtaagaaa agactgaaga atataattat taggccaggt gcagtgactc 33480acacctgtaa atcccaacat tttgggaagc tgagatagga ggatcacttg aggccaggag 33540tttgagacca gcctgggcaa catagtgaga ccccatctct acaaaaaaaa aaaaaagtaa 33600aaattacgtg ggcacggtgg tatacgcctg tacttccagc ttcttgggag gctgaagtgg 33660gatgatcaga ggacctcagg agtttgaggc tgcagtgagc tatgattgca gcactgcact 33720ccagccaggg agacagactg aggcccggtc tctaaaaaaa aaggaatcta tgtgagtcta 33780tatattcgtg caacttttct ataggattga aactctttaa aataagctta ataataaaaa 33840caaaagctgg tgggtgagac ctttcatgta ctctccagga gagttaagcc ccccaacatt 33900cctgtcccct tgtttactct caagcacccc ctcccccacc caaggaccag gtctttgttt 33960actgagcatc tcagcgatga gctctcaccc cctgatttca tcaattataa atgtgctcgc 34020tactcaccac acggcaattt gtgacggact gtggtttgtg gtgagagtag caccatccaa 34080gttcaccgca gccgcgagta gagatgaggg ttggggccag acacagggct gtgggggcgg 34140caagggcacg caggcagccc tgccaccttc ctgtttgtca gccaagtgag gcttccgagg 34200gcagcgggcg agcgggtcac tactcagggc cagctactgc ggccaggcca ggctagtcag 34260gtctgtgcag ccagaactag aggctccgcc aggatgtgag gtctcccagc tcctgggaac 34320tgaagcaaac actctgacta ccccttcttg aagtgcctta cggtgtatac atttgtttaa 34380tctgcacaac aaacctagga gcagttattg ttactgtcct cattttgcag atgaggaaac 34440tgaggcaaag agaaattaag taactccttc aaagtctggt aagtgacaga accagatttt 34500accctcttac tcattgtcca tattgccgag taacttacat taatagatac tatgcatgtt 34560tattttattt tttatttttt tttagagatg gggtctcatt cttttttttt tttttttttg 34620agacggagtc tcgctctgtc gcccaggctg gagtccagtg gggcgtgatc ttggctcact 34680gcaagctccg cctccctggt tcactccatt ctcctgcctc agcctcccga gtagctggga 34740ctacaggcac ctgccaccac gcccggctaa ttttttgtat atttagtaga gtcggggttt 34800caccgcgtta ccagtatggt ctcgatctcc cgaactcgtg atccgcccac ctcggcctcc 34860caaagtgctg ggattacagg cgtgagccac cgcgcccggt cctcattctt tcactcagac 34920tggagtgccg tcgtgcaatc tcagctcact gcagcctcaa ctcggggctc aagcaatcct 34980gccacctcag cctcccgagt agctgggacc acaggcagtg ccaccacacc caactaattt 35040ttgcattttt tgtagagacc ggcttttgcc atgttgccca ggctggtctc gaactcctga 35100gctcagacaa tccacttgcc tcggcctcca aaagtgctgg gattatagca ccacgcccag 35160ccgatactat gcatgtttaa ctgacaccta atgattaggt agcaacaatt tcttggctgc 35220cttattcacc ttcatgaccc ccaacattga aaatgtcttt tcccatgtga aagcatttgt 35280gggctcctac tgccaatcta taaagtagaa atccttctgt ggtacaaagc cctctttctt 35340atctaatttc ccatttcccg ttcccccttc ccccttctgc taagagctct tccctaccca 35400cattgtaagt agggccgggg agctaactcc accttgcgta ctccaaatca accacatgac 35460ccaggcctga ccaatcaggg ctccaaatcc tctagctata gtgattggtt tagggaggga 35520catgtgaccc gcctgagcca atgaggatct gttctgggac tcctgtttga actcttggga 35580aaataaactc cttatgttgg gtggctgagg ggatgaatgt gagctgggag ctgcaagcag 35640gcatcatgct attcccttca gctttcaagt agtgttttca ctgctgttaa caattccaaa 35700tcttaagtgt tccttgcatc ttcctctcgc caaaaatatt acagtactgg agggcttaca 35760tggtgtctca aatgtctgga gtttaaatct gccttgcagc ttccatggcc acagtgagtg 35820tctgagtcct ggccgctttc tgctgatccc ggggagagtt tagcctgcct gcccttcctc 35880cctggttcgt tctcatgtac tcagatccct gcctcactct tctctcacac agacaacaaa 35940agagagtcag attgtggtct taagtgcaca ggactctgaa gttaggacca gaagaccttg 36000gttagagtct aaaccttgtc agttaccaaa tatcattagg cacattagtt aatctctctg 36060aggcttattt ccccatcagt aaagtgggta ctgctgaaga tgtagtcttt agaagtgttc 36120tctagattgt aagccccgtg cttcctggtt attgactaaa cagggataaa aatgagtttc 36180tcctcacctg ggtgtcattg agtttctggt cattgtgaga tgggtgatga agccaagggc 36240atcaaacatc ccaacgaggc tgttttctgc atctccaggg ctatcccagc gttaatcaca 36300tatggacctt taatcagagt ttgcagaata agtgagaatt gtgttagagt atgaatgtag 36360atattatctt gttttagtca ttgttactct aggggaccat tcttactaca ggaatattgc 36420acaaaaccca gaaatttatt gactttctcc taaccaaggc ctaaagagct ggtgttagac 36480ataggccaag gccagccaga ggcccaaagc ctgtttccca gggtaggact gccctggcct 36540ccccctcttt ctccccaggc tccaccccag agagctgaag accaggctgg gtacggcact 36600gctgagaaac tgaggaaaag gccactggcc tcctctctca ctgcaggctg cccacccggg 36660agggggaaag cttgtcacta aatcaggttc agttttggtc actgtcttgg actggatatt 36720ctagcatcag aactgagatg tttcttgtga cttaaagtaa cttcaggact ctattctacc 36780taggattggg cagaaaagtt atgggcctgc gggagttcca attcagaaac aggggagatt 36840acttgcacta aagaaagtct aaaggaaggt aggagacaaa aataaagttg tgtattgatg 36900atcctaggag ttatgcttgt ttgacatacc agttatacct gctgtcacgg tagttatgca 36960ttaggggacc caggtgtctg aagttatatc cagaagactt ctgagggtgc accggggggt 37020cccttggcta aaagtgtgat ttaaacccta agagcctgcc cagactatca gtcccagttt 37080ctacgtccac tgtccctgaa tctcgctgct tcttccttag gctgctggga gtctgaaccc 37140tcccccgcca acacccctcc cccatgcctc agtcgtggga agggggggcc cttgagcagt 37200agggccaagc cctgttcagc ctgggaccaa gttcccatca acaaggtggt ctgggcagtg 37260gccagccaga aagcagtaat tactgtcgag gtgcagggac cccaggtagg gcccccacct 37320cccacctctg tgtgggcagt gaatgggcct gcccctgggt aaggctgtgt cagcaggcgc 37380ctgcccaccc cttgctgggt tcccaggccc ctagagccct ctcgtaatag gagccatttg 37440cgctgtaacc agtgggtgac cagattttta atcttggaga ccccttggat cccaggcggg 37500aagtgggatt tgtcaaatgg ggagaggcgg ggctgtctgg gaatgccaga cggggttgtg 37560ctggggaaat atgtctcctt tccatacagc ccccttccca tacctccagc ctccctctac 37620cccgcaagtc agctctgtag ctcctaggag gtatctccaa catgcttagc tgttgaaagt 37680aaatgaatgc cggaagttga aatctgaatg gcttgttttg cactaactca ggtgcatgcc 37740aaataggggt gtctctttgc ttgatcctaa tccttcctcc tctgaaatcc tttctgacct 37800gctgcctatc agcaattgcc ctgcaaagac cctctagctg gccgcgggag aagctgtgtt 37860cttggctatc aggagtgaga acactggacc caagcttgcc tattctctgc cacaactcac 37920tgtgtgatct tggacaagtc acttcctttc tttcagcctc agtttcagga gacttggcac 37980ttgctgagtg ccaatgtgtg ccatgttctt gacaagtatg gtcccacaca accttgccaa 38040tgaccctgtg acctgtgtct tcttgttccc agtttacagg agagaagatg cagcctggga 38100gagatgcagc aggtgtctga ggccacacag caagtcaccc agggccagga tctgaagctg 38160ggtctctcca gctccactgc ctgggcactt tctcctccac agcgaccttc aggtcatcat 38220gaggagcctt tcggactaaa gctagagagc tgggattcca acagttcagc aacccatgac 38280ttctccatgg cagctgctgc ctgaccacct agtgcctttc cactaagatt gttccttccc 38340tctcctgaag attatcctcc tgcccctctc tcccaaacat ctgcggtgtg cacctgctgc 38400ccaaagttga actttttttt gttagagaca gggcctcact gtgtcatcca ggctggagtg 38460cagtggcgtg atcatagctc actgcagcct ccaactcctg ggctcaagtg accctctcac 38520ctcagcttcc tgagtagctg ggactacagg catgcaccac cacgcctggc taatttttaa 38580attttttgca gagacagggt ctagctatgt tgcccaccct ggtcttgaac tcctgggctc 38640aagcaatctg tctgccttgg tctcccagct cgctgggatt acaggcatga atcaccatgc 38700ccggtcctcc aaagttgaac ttttgagact cagtttcctt cttggtaaat tcaggctcct 38760aggtgctacc tcctcagaga catcctcctt gaccacatca catgacctct cgcagaggcc 38820agattcttta atgtattcat ttgtctatta tcatgtttcc ccagtagaag ttgcacaaac 38880ggcaggggct tcatctgttt tgtttgccgt tatgtcctta gcacctaaaa ttgtgcctgg 38940cacatatagt actcagtatg tatttgctgg gtcaatgagt gagtgaattt atactaataa 39000cagcagctac catttctaga gtgtttacca tatattgggc actgtgtcag tcttcccaac 39060aacccacaga cgaagatcaa ttattacacc cattgtacag atcaggaaac tgagtcaggt 39120taagaaactt gccctaaatc ctacagtctc acttagaact tctgactgca gtgctcatca 39180gaatgcattg tcaacccaaa ggtcatttcc agctcaggtg gcttctatca aaagagctca 39240tcctggcctt tccaagagcc agacctccga catcggtgga gccctgtgca tagctggcct 39300ctcctgggcg tcttgtccca agtacagaga cctggatcct ttcccactca tgtgcaacag 39360cccaaaatta aaaacaaaag ccatattaaa aaacaaaacc aactttctgc cttaaaatat 39420tgtgagccag ggggcaatta gcaattatgc tgtattttat tatgagaaga tagaattcta 39480attggactga tttgaattcc acacacctcc acagattgtt ttgggaatta aggtatcagt 39540tgtatcggta attatggttt accattcaat taccccccca cagaaaactg ttaaattgtc 39600tgtgacgggg cttaaattta gctcagacct atgtcctatg aagactgcgc gagtcaatac 39660aagccatccg gaaaccaccg ggtgccctgt gccaggcggt aattaggggt tgaggtttcc 39720aaagttttac ctgagacagc agggacaagt gcctgggctg ggcgtgctca cgtggggggg 39780cttggatgct cccccagcac agtgctcttg gctcctgccc tgcgttgctg gtgcaatagc 39840tgatcatctg gaagacaatg tggtttcagc cgcaagtgac attttggcga ggtgcaccag 39900catagaagcc ctgagacagc gagggaccat gtaaaactca cggacattgt aattggacac 39960atctgggatt gaattcccac tcttccactt agggagtgaa cttgagaggg ccacttaata 40020tctttgagcc tctgtttccc cacctgtcaa atgggtacac ctcctgcctc acacacgggt 40080tattgtgaag atggatggag aataataatg gtgcctggcc caaattgcat ttttttctct 40140ttttttagta ctcactacat gccagatact ttacaaacat ctctaaacct cctaagccct 40200atagggcagg cactgtggtt attctctgtt ttatggatgg gaaaactgag tcccagagag 40260gttaaataac atgccgaaag tcccatgact atgaaatggg gcagctggga tccaaaccca 40320ggtgacctag ggccaaaccc taagcatgaa gctgccctgc tgggtgcctt ctgtacctgt 40380ccctctaagt ggaagtgcct agaaatgacc cagccaaaag cagcagactg tattttatca 40440tttcaacaat tctctctgtc cataaaaagt tctatgcagc ttggccagtt cctttttcct 40500gaatacaaaa ctgagaatgg taacagggaa ctgacttttt aatgtcatgg tgcaaaggag 40560ctagctctgc catgacctcc ttgaagtgac ctggtagagt gagggtaaga caagccacac 40620tccctaggct atggaggcca ctctccatgg agatagggga atagggaatc ctgcaaaata 40680cagtctctgg ggatgggaag gatcagggaa ggggccaggt gcagtagctc atgcctgtaa 40740tctcagcatt tcgggaggct gaggtgggag gatcgcttga gcccaggagt ttgagaccag 40800cagtttgaga ccagcctgag caacatatca agaccctgtc tttacaaaaa attaacaaaa 40860aggccaggcg tgatggctca cacctgtaac cccagtactt tgagaggccg aggtgggcgg 40920atcacctgag gtcgggagtt tgagaccagc ctggccaacg tggtgaaact ccgtctctac 40980taaaaataca aaaattagcc aggtgtggtg gctgtaatac cagctactcg ggaggctgag 41040gcaggagaat cacttgaatc caggaggcag aggttgcagt gagcccagat catgccactg 41100cactccagcc tgggagacag agtgagactc cgtctcaaaa aaaaaaaaaa acaattaaca 41160aattagatgg gtgtggtggt gcacagctgt agtcccagct actcaggagg ctgaggtgaa 41220gggatcactt gatccaggtg ttcgaggcta tagtaagctg tgatcacccc actgcactcc 41280agcctgggtg acagagtgag atcttgtctc aaaaaaaaaa aatttttttt tttaaaggat 41340cagggtaggt acatggactc ctcctattcc ttctggcacc atcagtggtt gaacacacaa 41400actttggttc aaatcctgcc tcttccaatt tgctagctgt gtgaccttga aaaagttact 41460taacccctct tatccttagt ttcctcatct tcagaataaa aatagtgctt gcctgatggg 41520ggagatgatg gatgtaaagg gcctcataga gggcttggca tttaccaagt gtttgagaaa 41580tattggctgt ttttattcaa ggcctgctca ttgctagcat gagctacctg caacagccac 41640ctctaggcct cttttgctcc ctatagtcca gccacaccaa gaaaccatga tggcctgaat 41700cctctctgac tttgtcaggc ctccgtgcct ttatacatgc tgttccttct gcctagaagg 41760cctttctctt ttttcaacct ccttgagtcc attttagtcc ctgccagatg gtcacttctc 41820tgtgatgctg ccctaactcc ttggcagcca tgctgctccc ttgaccattc actagcccct 41880ttaattcagc cccagccaca gtgaggatta aatgggatga tgtctgagaa agcccatggg 41940caagaacctg gcatggagtg gtgccccata aatgatgaat ggagctgggg ttgctttctc 42000agccagctgc ctggcagggg cctggcttcc tcatcttcac acgtcagtgc ctagagtaac 42060acctggcaca cagtgaacac aatctcagta actgttagac atttgtcttt tggggagctg 42120ctcagcctcc cacattgggg tgtgggtttt ttgttttgtt ttgttttgtt ttttgagacg 42180gagtttcatt cttgttgccc aggctggagt gcaatggtgc gatcttggct cactgcagcc 42240tccgctgcct gggttcaagt gattctccta cctcagcctc ccgagtagta gctgggatta 42300caggcacgtg ccaccatgcc tggctaatgt tttgtatttt tagtagagac aggatttcac 42360catgttggtc aggctggtct tgaactcctg acctcaggtg atccacccgc cttggcctcc 42420caaagtgctg agattatagg cgtgagccac cacgcccggc ctaggggtgt ggggtctttt 42480accgactagg cctggtgggg gaagtcaggg accccaccac aaaagttgga gaggccacat 42540cctcccacct ccatctgggg ttagacatgt gacccaggct tggccaatcg gatgttcctc 42600catcttgccc agaattcaca ctcagcagtg gtcttcacga ggggtggtag tggtggccag 42660taggagcagg gctgggcagc ctccctaacc cacgttcatg agacttgaag ctggctggtt 42720tcctgtcccc tggcctccct tgcccattcc catcctggtg ctccctccct atggactcct 42780tttgctcata aatatccctc caattaattg ctttgctgct tcttagctag tgttgtttct 42840gtcacttgca accaagagcc tattcaggtg caggaagggt tgaatgaaca atggaaatgg 42900atcaacacat gtttctgagc atgtgctgac aggcaggccc tgggcggagt accacggtgt 42960tcaaatgatg tgtaagatgt gggcagtgcc ccggggggtc tatggggaga cagaccatgc 43020atgacatcat aaacaagacc acattataaa gtggggttta gttggacctt gcagacaccc 43080agagaagacg actttgagcc ctcattggat ggtgccatcg caaagtacaa aacgaattgg 43140gttgtaggaa ctggaggcac tgggacaata ttccagatta gggattgagc tgaaggggtt 43200ggaaatggct gagtagggtg gtggaaaggg atgcctgcta gtaggcaatt gtcttagtct

43260gttttgtgct gctgtaatgg attaccacag actgggtaat ttataacaaa cagaaattta 43320tttggctcat ggttctgaag gatgggaaat ccaaaatcaa ggagccggca tctggtgagg 43380gccttcttgt tgcatcataa catgccagag ggtgtcacat ggtggaaggg caaagagagg 43440gagaaaggga aagagaggga gcaggaaagg gcaaacccac tcccatgata ataagctcac 43500tcatatcatg agtcattcat ccatcgtgag ggcagggtcc acctcccaat accatcacaa 43560tggcaactaa acctcaacat gatttttgga gaggacaaac attcaaacca tggcagcagc 43620gtttgtggga aacacgcgcc aaagcaagaa aagaccaacc taagagtgag tgacttggct 43680cctcattcta cattcatttt catccaatgg ggcccaaggg catgtaccca ttacccatct 43740gggcagttcc cttgaatgtg ggcttctgtt tgcccgtgga ggtgaggaac ttcaaggaag 43800aaaccatgaa agacctcttg aggctgaggg ctggcaccag caccaagatc tccaggcagc 43860tggaacagtg atggctcctc cgtcctcgca ggcggggcac ccaacagggt gtgaccgtca 43920cctgagggga gacagccaga ggcacaggcc tgatcctggg actgaggttg gcggtttggg 43980tggagaggtg attctgagtg tgacaccctc cagtgataaa gtggggggct tcccagcagc 44040ccctggggaa acaggctgca tctctggaga caggagatat gtggaggcct gaggggcagt 44100ggaaagccct gtgtgtctgg gtcaggtctc tccttggcag gtaatggttt gtcccagtag 44160ttttcagact ccccatcctg ccctgtcctt ctcctgtctg atgctcagac ctgtcactcc 44220cagctcagcc cccaccttac tgtctacccc tcagagtccc tcccaccaag ggcaccttct 44280gtcccacatc tccatggtgc agtcatggga ggagaacttg ggagcataag aaactccacc 44340aagttggggc caggcatggt ggctcacgcc tgtaatccca gcactttggg aggccgaggc 44400tggtgaatca caaggtcagg agtttgagac cagcctgacc aacataatga aatcccgtct 44460ctattaaaaa tgcaaaaaat tagccgggca tggtggcagg tgcctgtaat cccagctact 44520cacgaggctg aggcaggaga atcacttgaa ccgggaggca gaggatgcag tgagccaaga 44580tcgcgccatt gcactccagc ctgggtggca gtgaaagact tggtctcaaa aaaaaaaaaa 44640aagaaagaaa gaaaaaaaaa gaaactccac caagggcttt tgggtcctag ggacagcaat 44700gatgtggctg gtccagccag aacctcattt tgtcagtaaa gttgggaccc atattcccta 44760agagatttgt cccaagaaca catgggttgc tacagcggag gagaaatcaa gtctgttttc 44820cctctggtga attgtcccct gaacgtgctt tcttcctaca gtgttgccag gaaagtaaaa 44880aaaaaaaaaa aaaaaaaaaa aaagtccacg atgtcagctg gggtgatacc aaaacaattg 44940tgggaggaac aacatccgca aattgaatag tgtgaggagt gtggacagaa gatgttttgt 45000ctttggcctc atctcccaga cttgatcttt gtaaatacag aagtttccac cagagccgaa 45060cctggcaatg acttgaggag cagctgcaag gaagacagcc tctcccaggg tatcacctgg 45120gggcacaccc cagcttcccc tcctgagcct catcgagggg ttagtgctac ctctcgggaa 45180aacataaaga tgacaagaag ccaaaggtgc caatagttcc catttagtat aaaagctggc 45240tcagcaaatc atgctatttc agggcctagg gtgggccagt tcccaggcag ccctggcagg 45300aaggactctg agaggcggac agtaaggtag gggcttggga gtgataggtc taaacatctg 45360atggaagcaa aagggaggac aagggaggga gtcaaaaaat ccgggaagcc tgtccttcca 45420gaaagtaccg acaccagggt gaggtggatc ccagcacccg cacctctgaa tggcctttcc 45480cctgcacccc tatcccgccc ccactcggag ctaccccaag cacctgttcc tctgggcccc 45540aaggtgacgc cctttgtgtg tgtataggaa aggacggtgt acacatttgg ttagttcctt 45600cttttacacc ataaattatc agagacaacc cttttggaaa gcaatttggc aataatatca 45660agtgacataa agatgttcat agcctttggc ccaataatct tcctcctggg aattaatcct 45720aagaaaataa ttcacaagga agaaagaacc attttgtatc tacaaagaca tttattgggg 45780tgttatttat gatagggaaa aactggagac accctcccca gccaacatag agggatggtc 45840ctataaatta tgatacatcc atccaatgaa atgttaccct gccagtaaaa atggtaaatt 45900gaaaattgtg tagcaacaag aaagagtgtt cagaaaataa aagcaagtga aaaaaggagc 45960acaggattgt ctatatgctg tgatcaatgg caatgctgac aaatccatgt atgtgtagat 46020agttgtagtg ttacctgtaa ggcacgataa ggcggatgta actgtgcttc aaatgttctt 46080ttccatagct gtggagtcct tctagtatga aatcattttt gtaaaatcgc aatttgtcac 46140caggggtttt gactttcctt attgcctcgt gggaggtggc agaggcagca catctcagac 46200cgagcctcgg ttcctccccc tacctcccac cgctgcgctt gaagaaagat gctgcagcct 46260ccccagtccc catgccagcg ctcccacttt tctctgagct ttcggtggca gacagcgcct 46320tgggcacttt ttcatgctca taattcgaat tacctgttta agtcggtcaa atgaaaaaat 46380accagctccg cccccacgcg ggctggccgg ggcgcctgga gcgccagggc ggctgcagcg 46440cgctctccgc ggccgtcggc cctgagctca tttcctgggg cgcgcgcgcc gggctatttc 46500agcctggcgc tgtgcaaaca ggacaattta ctgcggccaa aagggaccca aattacaatc 46560gtatcacaga caaatatccg ccacgccagg tctccagggg ccaggagggg cctctctccc 46620ggcgcggggg gcgggcgcgg ggtcaggcag gtccgcgggg ctcggctcgg cctcgccgtg 46680ccctgatcgg cgtttgccac cgagctgtgc ctgctctctg caacaggaag gggcccagct 46740cccccgggcg accgttccta tctgagttct cgttcctatc tgggttttcg tgcagaaaaa 46800cttcatctct tcccagggat tttcccctga tttagggtcc ccttttattt ggtttctttt 46860cagcacttgg gatgaaaaca tccctccatc cagccactcc agggctcaaa gtcatatctc 46920ctcgttcaga aacctcagtg cctgtccacc actcttccgg aaaagtcgga atccttagtg 46980tgatggtcaa agaccgctct tagtccagtc cacctgtcca gggccagccc ctgtacccag 47040atgctccaac cacggagagc acatcagact ttcacaattt tatccttgac aattgccttt 47100tcatcctgcc taaatgttcc cttgtttctt tctgcctagt gagctcttat tccttcttta 47160agacccagat acacatcacc tcttccatga agccttccca gacttcccct ttccctgctt 47220gacatctgcc ttccaagcag agtcagcccc ttttccttcc tagcaccgtg cagaggtaga 47280tggtacttct cacatcctgt aattaattgc ttgcatgcct gtggtacacc gtaccattgc 47340tcacaaatat ttcctcttcc tctctggaga aggattacac ttccttgatc cattggcctc 47400actcaggctt cgccacatga ctggtgctgg ccagtgaagt gggacatgtg agttactggg 47460gagcagaaac tttcaaagcc aagcatggct caccttgttc tctcttccct ctgttacaag 47520gatggcagtg ccccagccag aaaccacact gccagcctgg cataggagtg gagcagggca 47580cacataagtg tgagagaaat gaacttcacc tcagtgctcg aggcccctgg gaccatggac 47640tcatttgtta ctgcagcata aactagctgg tcttgacttc agcttctgcc tctcttccct 47700ggctttgcct tcacaaaatc aagagtctga tcttactcat attatagagc tggtcttcaa 47760ccatgttctt tttttttttt tccttttttt ttttttgaga tggagtttcg ctcatgtagt 47820ccaggatgga gtgcaatggt gcgatctggg ctcactgcaa tctctgcctc cctggttcaa 47880gcgattctcc tgcctcagcc tcccaaatgg ctgggattac aggtgcttgc caccacgcct 47940agctaatttt tgtattttta gaagagatag gatttcacca tgttggccag gctatctcaa 48000actcctgacc tcaggagatc tgcccgcttc ggcctcccaa agtgctggca ttacaggcat 48060gagccaccat gcccagcccc atgttcttcg aatggccaag aatatcagta ggtaaggttt 48120gttgagcatg gattgtgtgc caggtgctgt gctatgcctt tggcaagcgt tatcccatta 48180catcctttaa cagcactctc tggtgggtac tgctcttata caagatttac aaaagagaaa 48240agagactcag aggaatgtag gggctcacgt gagattgtaa agtagtgaga agtggagctg 48300gtccttcagc tcaaatccct ctgatgccaa agaactcaaa ctctgcagtt ccatcatgtg 48360catcctcact ctctcacttc ctgcagagga tctgtggagg ttgtaactat tgttttcacc 48420tgtctcttcc actgggctct gagctgcccg aagacagatc tttttttttt ttttgagaca 48480gaattttgct cttgttgccc aggctggagt gcaatggcac gatcttggct caccacagcc 48540tctgcctctt gggttcaagc gattctcctg cctcagcctc ccgagtaact gggattacag 48600gcatgcgcca ccatgcctgg ctacttttgt atttttagta gagacggggt ttctctgtgt 48660tggtcaggct ggtcttgaac tcccgacctt aggtgatctg tctgtctcgg cctcccaaag 48720tgctgggatt ataggcgtga gccaccacgc ccggccccga agacagatct taatcatctg 48780atttcccatc cattaattca gtctttcacc aaggatgtat tgagtgccaa ttatatgcca 48840ggcatcatct gatgtgttgg ggacagagaa ataaaacaga taaaaatccc tgctgttaat 48900tctagcctat ggtgacaaga agcagatccg tggctgcctg gggtgagtgt ggggtaggaa 48960atgggagaaa tggggatgac tgcaactttg ggggtagtgg aaaatgttca gtatcgtggt 49020agtggtggtg gctgtacagg tatatatatc cgcatacatc ccacagttca gaattataca 49080ctttaaatgg taaatggata cagtttatta tatgtaaatg atccctcaat taagttgatt 49140tagaggaacc tggccgggcg tggtggctca cacctagtat tctcagcact ttgggaggcc 49200gaggtgggcg gatcatgagg tcaggagata gagaccatcc tagctaacat ggtgaaaacc 49260catctctact aaaaacacga aaacaaaaaa ttagccgggt gtggtggtgg gcgcctgtag 49320tcccagctac tcgggacact gaacctggga ggcggagctg gcagtgagct gagatcgcac 49380cacttcactc cagcctgggc aacagagcaa gactctgcct tgaaaaaaaa aaaaaaaaaa 49440gaacccttgc tcttatggag aagcattcta ctggggaaaa cagacaataa acacatagat 49500aaataaaaca tatgtcagaa aggggtaagt gatgctcaga aaaataggac aagggatcag 49560aagtgctagg cgttgcaatt taaaataatg gggtctggaa agccaccctg agaagggcca 49620atggagctat gcccagcctg tggtatcctt cctcaggcag gggactgtac ccccagctta 49680caagcctttt cgaggcacct tcagggtttg agcccaaaat gcactgcaca ttaagtgtta 49740ttgatgggct tgtggaaatc tgctctccgt caggctctga tgaaactttt ccaacaggag 49800actgaactca gtactgggca tggccccctg cacatagtaa gtccacagca aatgtgtgtg 49860gggtgaatac tgtcttcttt atcctccctt tttctactcc agtccaggta gacggtgtat 49920ctacccaccc aattttgctg tttctggtac cctcagtggg tcttgcctgc tcttcctcct 49980tgaaatcatt actcagaggt ctccgtgtca gctccagaca gatgggcctg gtgctccttt 50040atctcaatac aattcccccg ctcccaggct ccaggggaaa tcctgagcta actttggtct 50100ttcctaaact ccacacttcc ctttgggcac ctctttcccc attgcggggc tccctttcag 50160aatttgctgc tttatttatt tatttatttt tgtcagaggt gcattaatga tgctttattt 50220aaaaacaaaa aacttggcca ggcgcggtgg cttatgcctg taatcccagc actttgggag 50280gctgaggtgg gcggatcaca aggtcaggag atcaagacca tccagaccaa catggtgaaa 50340cccagtctgt actaaaaata caaaaaaaaa aaaaaaaaaa aatcagccgg gcgtggcaca 50400tgcctgtaat tccagctact caggaggctg aggcaggaga atcacttgaa cccgggaggc 50460agaggttgca gtgagccgag attgggccac tgcactccag cctgggtgac agagcgagac 50520tccatctcaa acaaaaaaac accacaaata aataaaaaat aaaaacaaaa aaccaaaaac 50580agtccattcc atgtcgtgtt gaaactgatc agtgtaagtt aaatggtggt ttttaggctg 50640gacccatgat ttaagctgta cccatccagc tcaaactgaa aaaaaaaaaa atcatttgaa 50700tgttaaagca atcgttcaga gtcttcaaga agaaaccagg caggaaaatg ccaataatga 50760tgactggcaa aatcaaaatc taaaacaaat aaactgttta tcaagctgcc gacagaaaaa 50820gaaatcttgc atggagacta caagtctgga ttttctggga tgaaattgta caggaatctc 50880agtctacagt ttcctcaatc gctgtggaga tggagctgtc actgaatctg acagagccct 50940gcactcccca gtccgccgac cctttctgta atccagtctt cactgtagcc tgaggaacta 51000tttcaacctg ctcctttttt atcttcttct ttggcacaac ctcagtggac ttctctgatt 51060cagaacaagt tctaattgat cttctctgtt gcttcttttc tactgagcct gtagaaccag 51120atgttgcttc aagagatgat atattctgca ttggcttttc atttctctgg tttggtttag 51180aaattataag cctgtcttgc cccctgacac ttatttctgt tttgttacca attccctttg 51240ttgaataaac aaattaattt cccatcctct gtagcattct gaagagcaaa cacttcttca 51300attttcacag ctggagacat gttacacttc tgcaaatcca ggctcccttt gtgcattgta 51360atggaagctg gtaggatttc cttgctgcca cagttttcca ggctatttta acaggaggtg 51420gctcttcctc gtccgcgctt gtgtgctgcc tcgggctgtg tctccaaatg tcagtacttg 51480agagtgagga ggccacctcc cctgcattga tctgttctgg ctgagtttaa agcacagatc 51540ttggtcatca ggttttttta acttcggctt tggagacaac attctttttt tttttttttt 51600tagatggagt ctcgctctgt cgcccaggct ggaatgcagt ggtgcgatct ccggttcatg 51660ccattctcct gcctcagcct cccgagtacc tgggattaca ggcgcccacc accatgccc 517193492DNAHomo sapiens 3ggtttcaact agttctggtg catagcagcc cccaaagttt tagcagcgtg gttagtggag 60agctgtggct tttgcctcca tctaaatttt tctttgagca aattacctct ctcctgacag 120ccctgttggg ggtgtcagtt gtggggttct ggtacctccc atcccggccc cagctagagc 180aggcaggccc acaggaattt gaatctggag ggagttatca gtggtggagc gtagaggctg 240caggaatctt catataagtg gagtcatata atatgtggtc ttttggtctg gcttctttac 300ttagcatgct ttcaaggttc atccatgtcg tgggatatat tagtgcttca tttcacttct 360gatggcaggg tcctgaatta agtcctgccc tgaggatcac actgttacat ctaggccttg 420taggattcct gaaaattgca tcccatctgc cctgctctaa aatctacaca gcctttgcca 480tatccattta tc 4924557DNAHomo sapiens 4ctagggctta gaagtttcct taacaccaac tgaggagtcc cagggtggga gctgagctca 60cacgaagctc tacttcgcgt gtcctgaagc tttgacagtt gggcctcttt ctggcttttg 120catcctctgc tcatactaag gccagcagag ctacaggttg gaccatggtc tggccgggag 180ctccagcttc ctcttttcct ctcagtagtc atcgggccag ctgcccatac ctggtgccca 240ggtatgagaa gagacctttg gctttcacca ggttcttgag agggtcaggg acctccaaaa 300ggataaatgc cattggtcag atagtcactg tctacctccc tcccatccat ttcctgcctt 360caacccctgt tcttgggaca aggccttcgc tggcattcaa tggcgggtcc ctgaggaagg 420ccccagcctt gccgaacatc cttcaaggga gacttcagcc cctggccctg cagtgagtgt 480gttgttagca gcagaggggc tgagtgaggc agtgggatgg ggcttttctt ttttgttctg 540gttgcaaatt ataaacc 5575819DNAHomo sapiens 5gccttcagtt tgtcccgttc taaccgcccg gccgtctcaa tgcgatcaca ggagggacac 60ggagatcgag gagggccaca gccggaccag gccaggcgtg ctgcggcagg aggaaggctc 120cggaggtggg gtgggaaggg gaggccgaga gacgggtgtc gccgcgcccc cgctgccgcc 180agagaggagc ctacggctgg cagcctggcc tgggcagcag ggtcctcggc gctcggctgg 240gaaatcgcac gtctctccgc ggtgacctgt gcacagcccc tgggcctccg cctccgtgct 300ggcagcctcc gcctcagcgc acaaagcccc gtcaccccga ctctcggagc gccgccgccg 360ccaaatcctc agcccctccc tcattggccg cggcgtctgc cgggaagtgc agtcccgggt 420ttggggcgat ggagcccagg aggaagcggc gagtcagcgc ggcggagagg gcggagggga 480cggagggggc ggaaggggac gaaccacgaa cgcccgcggc cgcgaagggt ctggacgaca 540aaggagagac tagcgagagg gcttgttgct tttttttttt tttttttttt tttttttttt 600gagacggagt ctcgctctgt cacccaggct ggagtgcaat ggcgcgatct cggctcgctg 660caacctccgc ctcccgggtt caagcgattc tcctgcctca gcctccgagg agctgggatt 720acgggggcgc gcctccacgc ccagctaatt ctttgtactt ttagtagaga tgaggtttca 780ccatgttggc caggctggta tcgaactcct gacctcgcg 8196581DNAHomo sapiens 6ccctgccatt ctggatagtt ttggttattt ttagtttaac ttcaggtgag ccactcttct 60ttggaatcac agtttgtggt accgaagtgg tggctgtgga ggggaagggt tttctagaat 120ttaatgtggt cttctgttta gaatttctga atggtaatag tcagtggggc taaagtttct 180agctgcgcca aagtcattgg ctgacccatt aggatattga ttatgcgact ggtatttggt 240ttgaatttca tacatgctga ttgatggcag gtagccattt gtgagtggag gaagatctgt 300tgtaggtgag tattgaaagc tttgctgcaa ggtagcttcg tatggtgtct ggccaccatc 360ttcagcaatg tcacttttgt tatcaaaggc atcctcctga cggatgctgg cggagtcagt 420gagttgaggt ggttgttgaa ctgtgtttcc cgtgatccct tgcatgaaag agaaagagaa 480atccattgtt ctgctccagc attcttaact ttccctttct ctcatcgggc cttcagtttg 540tcccgttcta accgcccggc cgtctcaatg cgatcacagg a 5817602DNAHomo sapiens 7attccctaag agatttgtcc caagaacaca tgggttgcta cagcggagga gaaatcaagt 60ctgttttccc tctggtgaat tgtcccctga acgtgctttc ttcctacagt gttgccagga 120aagtaaaaaa aaaaaaaaaa aaaaaaaaaa agtccacgat gtcagctggg gtgataccaa 180aacaattgtg ggaggaacaa catccgcaaa ttgaatagtg tgaggagtgt ggacagaaga 240tgttttgtct ttggcctcat ctcccagact tgatctttgt aaatacagaa gtttccacca 300gagccgaacc tggcaatgac ttgaggagca gctgcaagga agacagcctc tcccagggta 360tcacctgggg gcacacccca gcttcccctc ctgagcctca tcgaggggtt agtgctacct 420ctcgggaaaa cataaagatg acaagaagcc aaaggtgcca atagttccca tttagtataa 480aagctggctc agcaaatcat gctatttcag ggcctagggt gggccagttc ccaggcagcc 540ctggcaggaa ggactctgag aggcggacag taaggtaggg gcttgggagt gataggtcta 600aa 6028572DNAHomo sapiens 8gagaggacaa acattcaaac catggcagca gcgtttgtgg gaaacacgcg ccaaagcaag 60aaaagaccaa cctaagagtg agtgacttgg ctcctcattc tacattcatt ttcatccaat 120ggggcccaag ggcatgtacc cattacccat ctgggcagtt cccttgaatg tgggcttctg 180tttgcccgtg gaggtgagga acttcaagga agaaaccatg aaagacctct tgaggctgag 240ggctggcacc agcaccaaga tctccaggca gctggaacag tgatggctcc tccgtcctcg 300caggcggggc acccaacagg gtgtgaccgt cacctgaggg gagacagcca gaggcacagg 360cctgatcctg ggactgaggt tggcggtttg ggtggagagg tgattctgag tgtgacaccc 420tccagtgata aagtgggggg cttcccagca gcccctgggg aaacaggctg catctctgga 480gacaggagat atgtggaggc ctgaggggca gtggaaagcc ctgtgtgtct gggtcaggtc 540tctccttggc aggtaatggt ttgtcccagt ag 5729606DNAHomo sapiens 9gaagcttctc catgttctta gccaggttat ataacttctt ttgcttcttt cttttccaca 60ttctacatat ttttcacaat gaggatgtgt cacttctata gttaaaaatg gaaatcttca 120tttaaaagaa caatcagaca caaggcaagg tatgcttacc ttcccaatga ccaaggagca 180gggagacatt ggtcgtggag gccataggtg accagccttg agggaaggga aggaagggga 240ggtgcaggga ggcgcagcag aggcacccga gtgtgctcta cgaatgtaag tctgccagct 300gctcctctgt gcctagcacc ctggaaagcg cccgcacaca gtgggccctc agcaaactcc 360cactgagcaa agggccctgt gagtaaggac ctagaagcag ggtgtgcttg aggcatgggg 420tgggagggca tccagcttgg ctggcggtgg gagccgatgc aaaggtgagc tgagaccaga 480cggtagaaga ccttcagtgc tgggccgagg agggttcctt catcccatag agaagagctg 540ccatccaaga cagaaggctg gggagtgaca tgttgaaatc agtatcttag gaaaataaag 600ccactg 60610664DNAHomo sapiens 10gccataaaat caggataatg gtggctgtgc ctccttagag tagaatgaga atcagagcag 60aacaagggaa agctgcagtg acttgtcagg tgtcaacctt cagcatgatg ggagagccat 120gggacccttc ccctttccct aagagagcca gccctcacag caggcctggg atccaatgcc 180cagcacccag ctgggagccc agggaccttg gccaaaatct ggttctgctt caacttggtg 240cccggccttt gtcaagtcac ttcctcattt gcaaactggg agagtttgga tgaaactatt 300gaatgaaatt attttggggg tttctttctg gctctcacag tcctcgcatg ctcaccatgt 360tcccttcaat ttcattagca cagcccaaca aagggttaag cagtggcagt tcctctcgtt 420ctttggttag gacaggaagg tcaggggtga ggccaataca agaggtagcc gccacagctg 480atgctggaaa tgacaatagt tctttcctag actcatattt gtcccctctc cctgaagctt 540tgcctgcagt gcccttgtaa agaagttggc aagaagcagg agtgaggctc agcccctctc 600tgaaatggat acgccggttg ctccccctca tggctggtct catttgcctt cttcattttt 660agac 66411688DNAHomo sapiens 11tgcctagaac attctggtat cccagacaga agtgcaacac caggtggagg tgttgcagct 60gagaagttct gaccagcata ccagcacccc cttaaaagct gtctatactt gcggttctca 120gctaggctgc cacagaatcg cccaagtctt aaaaaaacag gtctctgtcc tactgcttga 180gatgctggtt ctctaggtct gaggttctgg cacctgtgtg tttttaaaac tttacaaata 240atttaaaagc gccttcaagt ccacaacaac agaatatttc cctccactcc tcattgtcct 300ggagttctct aacagtgcag ttctacaact ggacacacga tgtcgctttt cagccacagt 360tctcactaag cggccccaca gggcggcagg tgccttctgc agagagagag agagggcctt 420ggctgacagg ccaagaccgg gcatcctggc tcctcctctg tacagacttt tcacagatgt 480gaactctccc tactccctgt cttctgcccc caaatgaagc ctctcagctg gcaagagctg 540agaactacca agcgagccat tgctaatttc tattgtgttt ggaaccacaa aaggcagaat 600tattaaggct gtaaaggacc tcagagcatc tggtgcagtg agtttccaac ttgtgaaaat 660ctgatgtgat ctcggacaag tcacttaa 68812550DNAHomo sapiens 12caaagtggct ctgattattt cactcccaca ggccactggc tcagaggtat agagctcacc 60tgtggcagat ggagatgcgg atctgaggct tctgatgctg ccacacccag cggcgccccc 120caaattccgg gcccctggat gacatctggt ctgttcctgc agcatcagag cacaatagag 180ccagccacca gtcccagccc tgcctgcatc ccatccattc ctgggtgcct aaccccgagg 240atcccctggc agtatgatgc ggacctgtct tggatcccag ggatatgctg gccacgggga 300ggagccggaa accaaccttt gtgtcactgt gtagtgacaa gtgcctttgg aggtcacaat 360agccagtggt gatttctacc actgccccca gcagccaagg tggcagagga gccctgtcag 420tcacccccat tctgttcatg gtctcacggt gggctccaca

tggggggtgg cagccctctc 480ccccacccca cccgacccct ttcgacagat agggtaatac aaatacaaat aacaccaaaa 540gattgagttg 55013531DNAHomo sapiens 13ggatctccta cgtactgcta cgactgcaca caggtgcagg aaatggctgt ttgctttgcg 60tttgaggaac ttggaaaggg agacgtggta gggaaagtag tggttagggg cactttcaga 120ctgaaggatg tgggttgggg aatacgggat tcttggagtt gaggacgccg cttctctcgt 180ctctagctaa tgtgagaaag acccttctgg acactaagcc tgcaattcca ctggtggcta 240ccaggtgtcc gtggtgtcct ggggcgggtg taatgagagc gggagcctgt gaaaccaaaa 300gcattgtttt tataaattca gcactcttca atccctatta ataaggttag cggtgcagtt 360cttgcgtctc ctgccctgcc tcaccttgca atcatattca ttggcattcc tttcttccaa 420gaacccacct aggaggcctt gcaggagata tctctggtgc tggctgttcc tgcagtctga 480aaagccagtt aagatacaaa tatgtgagag gacactgctt gaatctgatt t 53114673DNAHomo sapiens 14tgagggtaaa atgaaacaat aatcacgggt gcatcctgga gctctttctt acaaggcgtg 60cccccaaatc tgtcccctct ttctgaggat gcccttcccc tattgtctcc ctggccattt 120cctacccatt ctcaagggcc atgatctcag ggagttctcc tgactcaccc aggcatattg 180gatctcctac gtactgctac gactgcacac aggtgcagga aatggctgtt tgctttgcgt 240ttgaggaact tggaaaggga gacgtggtag ggaaagtagt ggttaggggc actttcagac 300tgaaggatgt gggttgggga atacgggatt cttggagttg aggacgccgc ttctctcgtc 360tctagctaat gtgagaaaga cccttctgga cactaagcct gcaattccac tggtggctac 420caggtgtccg tggtgtcctg gggcgggtgt aatgagagcg ggagcctgtg aaaccaaaag 480cattgttttt ataaattcag cactcttcaa tccctattaa taaggttagc ggtgcagttc 540ttgcgtctcc tgccctgcct caccttgcaa tcatattcat tggcattcct ttcttccaag 600aacccaccta ggaggccttg caggagatat ctctggtgct ggctgttcct gcagtctgaa 660aagccagtta aga 67315570DNAHomo sapiens 15aaagacactg cagagaaaag aaagcacagc ctgctgccct gggaattaac atgatttagg 60agacctgcag gtcaccccct catgactaaa agccatcctg gaatgaaggt ctgtggctat 120ttctaggcaa aactgtctga taagataaaa tagctcaact cctgaccatt aagtcgtgaa 180ggccatggcc atcgtaaatc tcatctttcc ggccctctgg cctgcatgca gtgcagccca 240gccagtcggt ggcagccacc ttggtaggaa gggccctcat cctcctggct gtgccccaag 300gactgggcag gcttcggtgc caagggtagt gcgagcactt gaaagccgcc ctgtatgttt 360attgttttcc ccaggtgatc cagaattact cccgaactct accagctgaa atcctcctca 420actcacatca gacaagacgg ccctgccact tacctgtcag atcactttgg gcaggtaagc 480tcattttcct gaatctttac ttccacacct taaaatgtga gcaatactat ctccctggca 540aggttgtttg tgagggtaaa atgaaacaat 57016517DNAHomo sapiens 16actcctagaa ccctaaagga agcccctgga cacgcaggaa ggtgtggaga ggagttctca 60tacttgcact tgggaggagg gctcaggaga aacagaggct ggcaacaccc cctcacacac 120tggtcctctg gagggccagt gtctacagac actgtggact gagtccacag agaggaaagg 180gtcctgcctt catcagaact gctcagcaag cagttccatc ccagggggtc ctgcgaggta 240agggaggggc agctagctag gtgaggggct gagagagtgg ggaggggaaa gagggaagaa 300gagagtgaga gggagaggga gggactgagc cgattctcag ctccttgacc gtttgctgag 360ctctgtctga gtggacagat ggtcccaagt caggccacac cagagtggcc tttctgctcc 420cctacaccct gcattcctca acattgctgg ccccggagag actttccttc agagaagcaa 480atggctgggg aatggtgaaa gacactgcag agaaaag 51717586DNAHomo sapiens 17gtatcaccag tgaagttggt gcacacagca gggcccaaac tgacatctca cagcacccag 60caggtcactg tgggcatgat aggatgatgg gtcactgtgc cagccctgaa ggagttcaag 120tccagatagg ggaaggtggt ggaccagacc cagacagaga ttctgagtcg ctgctgagac 180tgggtgaggg tagtgggtac atgggaggac atatagcccg gcagcccagg gctggagtcc 240acactcaggt tggggcagcc tggtctgcct ctcctgcagg agacttttcc aggcaggctt 300gtccctccag aatgcacgaa tcaaatcctc tcaggatcag tctcattttc ctcgtgctgg 360gggagcaggc tactcacaga agatgttgtt gcaaatgtaa gaatcacatg tcgatccaca 420aactggcatt gagcagctac ctaggagatc aaagaaactc ttactttggg agctcctgcc 480agggctcttt gggaggtctg gctagctctg gaggaagaga atgaacttgg ggagggcgtg 540gaacagatga ggacgcaggc actgccattc aaagaggaga ggtctc 586181100DNAHomo sapiens 18cacagctgga gacatgttac acttctgcaa atccaggctc cctttgtgca ttgtaatgga 60agctggtagg atttccttgc tgccacagtt ttccaggcta ttttaacagg aggtggctct 120tcctcgtccg cgcttgtgtg ctgcctcggg ctgtgtctcc aaatgtcagt acttgagagt 180gaggaggcca cctcccctgc attgatctgt tctggctgag tttaaagcac agatcttggt 240catcaggttt ttttaacttc ggctttggag acaacattct tttttttttt ttttttagat 300ggagtctcgc tctgtcgccc aggctggaat gcagtggtgc gatctccggt tcatgccatt 360ctcctgcctc agcctcccga gtacctggga ttacaggcgc ccaccaccat gcccggctaa 420tttttttgta tttttttagt agagacgggg tttcaccgtg ttagccagga tggtctcaat 480ctcctgacct tgtgatccgc ccgcctgggc ctcccaaagt gctgggatta cagacgtgag 540ccaccgcgcc cggccccaac attctttttt gcttgggata aaccctcttc aggctgttaa 600tcaatataga taaaagtata ctgttctatt ctttcttctc aagtcatttt caatgctttc 660tctgcatggg caatgccaaa atcccattga gcatgttctc tctgaggtca gggtttccaa 720atcttttgtt tctcagagtg attgctggct tgtttggttg cctcagccag taattcttca 780taccgcttat gacctttata ctcctgtacc catttttcat gaacccacac cctctctggc 840tgtttgctaa aacactggac atgatatttt cgggcacctc ctgtgttaat tttggtatga 900acctccagct ggggatcact tcaatccata caaggccagc acggataggt tcccaccttg 960gaccacacaa gatcaccaac ttgaaactta acaccagtgg acacttctgt tgttggaaca 1020gaagatagta ttggctgaac tggggcttcc tcttttagta ctggatcttc ccttggtttt 1080tctgatatgg tgtgaaccct 1100191236DNAHomo sapiens 19gagctgagat cgcaccactt cactccagcc tgggcaacag agcaagactc tgccttgaaa 60aaaaaaaaaa aaaaagaacc cttgctctta tggagaagca ttctactggg gaaaacagac 120aataaacaca tagataaata aaacatatgt cagaaagggg taagtgatgc tcagaaaaat 180aggacaaggg atcagaagtg ctaggcgttg caatttaaaa taatggggtc tggaaagcca 240ccctgagaag ggccaatgga gctatgccca gcctgtggta tccttcctca ggcaggggac 300tgtaccccca gcttacaagc cttttcgagg caccttcagg gtttgagccc aaaatgcact 360gcacattaag tgttattgat gggcttgtgg aaatctgctc tccgtcaggc tctgatgaaa 420cttttccaac aggagactga actcagtact gggcatggcc ccctgcacat agtaagtcca 480cagcaaatgt gtgtggggtg aatactgtct tctttatcct ccctttttct actccagtcc 540aggtagacgg tgtatctacc cacccaattt tgctgtttct ggtaccctca gtgggtcttg 600cctgctcttc ctccttgaaa tcattactca gaggtctccg tgtcagctcc agacagatgg 660gcctggtgct cctttatctc aatacaattc ccccgctccc aggctccagg ggaaatcctg 720agctaacttt ggtctttcct aaactccaca cttccctttg ggcacctctt tccccattgc 780ggggctccct ttcagaattt gctgctttat ttatttattt atttttgtca gaggtgcatt 840aatgatgctt tatttaaaaa caaaaaactt ggccaggcgc ggtggcttat gcctgtaatc 900ccagcacttt gggaggctga ggtgggcgga tcacaaggtc aggagatcaa gaccatccag 960accaacatgg tgaaacccag tctgtactaa aaatacaaaa aaaaaaaaaa aaaaaaatca 1020gccgggcgtg gcacatgcct gtaattccag ctactcagga ggctgaggca ggagaatcac 1080ttgaacccgg gaggcagagg ttgcagtgag ccgagattgg gccactgcac tccagcctgg 1140gtgacagagc gagactccat ctcaaacaaa aaaacaccac aaataaataa aaaataaaaa 1200caaaaaacca aaaacagtcc attccatgtc gtgttg 1236201171DNAHomo sapiens 20accgtgcaga ggtagatggt acttctcaca tcctgtaatt aattgcttgc atgcctgtgg 60tacaccgtac cattgctcac aaatatttcc tcttcctctc tggagaagga ttacacttcc 120ttgatccatt ggcctcactc aggcttcgcc acatgactgg tgctggccag tgaagtggga 180catgtgagtt actggggagc agaaactttc aaagccaagc atggctcacc ttgttctctc 240ttccctctgt tacaaggatg gcagtgcccc agccagaaac cacactgcca gcctggcata 300ggagtggagc agggcacaca taagtgtgag agaaatgaac ttcacctcag tgctcgaggc 360ccctgggacc atggactcat ttgttactgc agcataaact agctggtctt gacttcagct 420tctgcctctc ttccctggct ttgccttcac aaaatcaaga gtctgatctt actcatatta 480tagagctggt cttcaaccat gttctttttt tttttttcct tttttttttt ttgagatgga 540gtttcgctca tgtagtccag gatggagtgc aatggtgcga tctgggctca ctgcaatctc 600tgcctccctg gttcaagcga ttctcctgcc tcagcctccc aaatggctgg gattacaggt 660gcttgccacc acgcctagct aatttttgta tttttagaag agataggatt tcaccatgtt 720ggccaggcta tctcaaactc ctgacctcag gagatctgcc cgcttcggcc tcccaaagtg 780ctggcattac aggcatgagc caccatgccc agccccatgt tcttcgaatg gccaagaata 840tcagtaggta aggtttgttg agcatggatt gtgtgccagg tgctgtgcta tgcctttggc 900aagcgttatc ccattacatc ctttaacagc actctctggt gggtactgct cttatacaag 960atttacaaaa gagaaaagag actcagagga atgtaggggc tcacgtgaga ttgtaaagta 1020gtgagaagtg gagctggtcc ttcagctcaa atccctctga tgccaaagaa ctcaaactct 1080gcagttccat catgtgcatc ctcactctct cacttcctgc agaggatctg tggaggttgt 1140aactattgtt ttcacctgtc tcttccactg g 1171211007DNAHomo sapiens 21acggtgtaca catttggtta gttccttctt ttacaccata aattatcaga gacaaccctt 60ttggaaagca atttggcaat aatatcaagt gacataaaga tgttcatagc ctttggccca 120ataatcttcc tcctgggaat taatcctaag aaaataattc acaaggaaga aagaaccatt 180ttgtatctac aaagacattt attggggtgt tatttatgat agggaaaaac tggagacacc 240ctccccagcc aacatagagg gatggtccta taaattatga tacatccatc caatgaaatg 300ttaccctgcc agtaaaaatg gtaaattgaa aattgtgtag caacaagaaa gagtgttcag 360aaaataaaag caagtgaaaa aaggagcaca ggattgtcta tatgctgtga tcaatggcaa 420tgctgacaaa tccatgtatg tgtagatagt tgtagtgtta cctgtaaggc acgataaggc 480ggatgtaact gtgcttcaaa tgttcttttc catagctgtg gagtccttct agtatgaaat 540catttttgta aaatcgcaat ttgtcaccag gggttttgac tttccttatt gcctcgtggg 600aggtggcaga ggcagcacat ctcagaccga gcctcggttc ctccccctac ctcccaccgc 660tgcgcttgaa gaaagatgct gcagcctccc cagtccccat gccagcgctc ccacttttct 720ctgagctttc ggtggcagac agcgccttgg gcactttttc atgctcataa ttcgaattac 780ctgtttaagt cggtcaaatg aaaaaatacc agctccgccc ccacgcgggc tggccggggc 840gcctggagcg ccagggcggc tgcagcgcgc tctccgcggc cgtcggccct gagctcattt 900cctggggcgc gcgcgccggg ctatttcagc ctggcgctgt gcaaacagga caatttactg 960cggccaaaag ggacccaaat tacaatcgta tcacagacaa atatccg 100722198DNAHomo sapiens 22ggctggagca gggacttgaa aaagggagag ggctcaggag actcagagga ggaggaaagt 60gtgtgagcag taggcagggt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgcacgc 120gcgcgcatgc aggcctgtgt agggctggga aagaacaaag caaaagggtg cacaaggcat 180ccagaagcca gggcaatg 19823369DNAHomo sapiens 23gagcagaagg ccttgactga gccccaggag aggcaggaca ccaggggtgc acacccataa 60acacacacat acacatgtat gtctcctccc tggagcctga gagtccctat atacagcagg 120tgcatgtggg ccacacatca cacaaaattg aatacaggca ggctcagagc accagcacac 180acgtatgtcc ttgacaccct tagagatact actaagcacg tgtgtgtacc tgctcaccca 240tatggcagag cccctggatc tgggcagaaa tgccaaagca ggggcaggcg cgtgtgcgcg 300cacacacaca cacacacaca cacacacaca ctagcacagc cacaaaagct caatccacat 360ccagcattc 36924436DNAHomo sapiensmisc_feature121,153n = A,T,C or G 24tcaggacgtt gcactttgac agaaggctct ggaaggaaac tttaaaggga gccttccaga 60gggaaatgcg gtgttggggt aggtctgcct ttggctatgg gctttctggc tgccggaggg 120ncccagggtc ccccaggaaa gccttctgtg ganggtcttt tgagagagac aaagcagagg 180ggtggaggaa gggcggctca ggtggaagga gtgaggacaa aggtgagtgc ccctgggcag 240gaagtgctga aagagagaag gagggaggcc accaggcctg ggcctggagc cagcctggga 300gactcccagc cgcccacttc tcggggcctc ccttttccag ccccttgctt tcgaggcagc 360agtgccatta tttggggaaa ccagctaacc agataggaca gcaaaccggg gatttatgtg 420gtgtgggaac agctca 43625670DNAHomo sapiens 25ctgggattac aggtgtctgg caccatacct agctaatttt tgtattttta gtagagatgg 60ggtcttgcca tgttggccag actggtttcc aactccccac ctcaggtgat ctgcccgccc 120tggcctccca aagtgctggg attacgggca tgagccactg tgcctgacct cagctctgtt 180attaataagc taaatggctt tgagcgactt gccttatcac ttgagcctca gtttcctcat 240ctgtaaaatg gggataaact tcttccgtcc gcatgaggat gctgagagac gtgagtgagg 300tggtctatga aagctcttgt catagcctgg catgcagggg taacatctgg atgatgaaga 360tgatgatacc tgagattttt gccttacaga caactccaga gagccctgtg aaatatttat 420atgccactga acagggcaca agatgaagcc attagcctgc gcttacatag tagaatgtgt 480gaatcagatg agatgcttgg tctctagtaa gaccttaagg gatggacaga agacaggcag 540attttggata tggtatacgt ggctgtgggc tagcgtgttt actactgggc ctgggatgta 600tttggaatgt acacatgtgt cctttgcttc tcagaacact ttgaggcagc agagttacta 660ctgcctgcca 67026460DNAHomo sapiens 26gaggttagac gggtctgagt tcaagcccca ggtctgccac ttcctggctg tgtgccctgg 60aacaagtcac cttatctctc tgaacttctt tttttttttt ttctttttga gatggagtct 120cgttctgtcg cccaggctgg agtgcagtgg catgatctca gctcactgca agctccgcct 180cccgggctca cgccattctc ttgcctcagc ctcccgagta gctgggacta caagcacccg 240ccaccacgcc cggctaattt tttgtatttt ttagtagaga cggggtttca ccgtgttagc 300caggatggtc tcaatctcct gacctcgtga tccgcccgcc tcggcctccc aaagtgctgg 360gattacaggc atgagccacc atgcctggcc atctctctga acttctgttt cctcatctga 420gatgacagtc agagtgggat ctgtgtaagg cactttgcac 46027482DNAHomo sapiens 27tgccaattag ccacactctt ctctagagag gtttcaagtc atttttcttc acagcaatgg 60caaggctctt aaataaggtc cttgaagttt cttcgggtct cctcccacct gctccctgcc 120cccttcacct ccacccacct gcttcccttt ctcatccccc agaggcggag gcttccgagg 180aatttggggt agggaaatag gaatcagggg ctcctcattc cccaaaggag cctctttggc 240aagcaggcac gtgggtctcc gggctggtgc acatcacagg gcagccagcc caagtgccat 300cttgatgccc aatcagtctt cctcatggct gcgccctgct ggtctctcag agggttaatg 360caatttcttg gaggacgaca ttcctaacac ccaggggcca gaactccttc cccactggtt 420attcccatgg cccagagcag caggatgggg gcagaaacag gcatggacct taacagcagc 480gt 4822816DNAArtificial SequencePCR primer 28attggcctgg cttctg 162916DNAArtificial SequencePCR primer 29ggctggagca gggact 163020DNAArtificial SequencePCR primer 30tcctgtgatc gcattgagac 203121DNAArtificial SequencePCR primer 31ccctgccatt ctggatagtt t 213220DNAArtificial SequencePCR primer 32cagtggcttt attttcctaa 203320DNAArtificial SequencePCR primer 33gaagcttctc catgttctta 203420DNAArtificial SequencePCR primer 34gtctaaaaat gaagaaggca 203520DNAArtificial SequencePCR primer 35ttaagtgact tgtccgagat 203620DNAArtificial SequencePCR primer 36caactcaatc ttttggtgtt 203720DNAArtificial SequencePCR primer 37aaatcagatt caagcagtgt 203820DNAArtificial SequencePCR primer 38tcttaactgg cttttcagac 203920DNAArtificial SequencePCR primer 39attgtttcat tttaccctca 204020DNAArtificial SequencePCR primer 40gccataaaat caggataatg 204120DNAArtificial SequencePCR primer 41tgcctagaac attctggtat 204220DNAArtificial SequencePCR primer 42caaagtggct ctgattattt 204320DNAArtificial SequencePCR primer 43ggatctccta cgtactgcta 204420DNAArtificial SequencePCR primer 44tgagggtaaa atgaaacaat 204520DNAArtificial SequencePCR primer 45aaagacactg cagagaaaag 204620DNAArtificial SequencePCR primer 46cttttctctg cagtgtcttt 204720DNAArtificial SequencePCR primer 47gagacctctc ctctttgaat 204820DNAArtificial SequencePCR primer 48ggtttcaact agttctggtg 204920DNAArtificial SequencePCR primer 49ctagggctta gaagtttcct 205020DNAArtificial SequencePCR primer 50actcctagaa ccctaaagga 205120DNAArtificial SequencePCR primer 51gtatcaccag tgaagttggt 205220DNAArtificial SequencePCR primer 52gataaatgga tatggcaaag 205320DNAArtificial SequencePCR primer 53ggtttataat ttgcaaccag 205420DNAArtificial SequencePCR primer 54attccctaag agatttgtcc 205520DNAArtificial SequencePCR primer 55gagaggacaa acattcaaac 205620DNAArtificial SequencePCR primer 56tttagaccta tcactcccaa 205720DNAArtificial SequencePCR primer 57ctactgggac aaaccattac 205820DNAArtificial SequencePCR primer 58agagagggtg agtaacttcc 205920DNAArtificial SequencePCR primer 59aataaaagaa agtttggggt 206020DNAArtificial SequencePCR primer 60gcagagtgct tttagaacat 206120DNAArtificial SequencePCR primer 61aggtggaggt tacagtaaga 206220DNAArtificial SequencePCR primer 62aagcagtatc tctgaagctg 206320DNAArtificial SequencePCR primer 63ccttttcttg gttcagataa 206421DNAArtificial SequencePCR primer 64acggtgtaca catttggtta g 216521DNAArtificial SequencePCR primer 65cggatatttg tctgtgatac g 216621DNAArtificial SequencePCR primer 66accgtgcaga ggtagatggt a 216720DNAArtificial SequencePCR primer 67ccagtggaag agacaggtga 206820DNAArtificial SequencePCR primer 68gagctgagat cgcaccactt 206921DNAArtificial SequencePCR primer 69caacacgaca tggaatggac t 217021DNAArtificial SequencePCR primer 70cacagctgga gacatgttac a 217121DNAArtificial SequencePCR primer 71agggttcaca ccatatcaga a 217219DNAArtificial SequencePCR primer 72acgctgctgt taaggtcca 197321DNAArtificial SequencePCR

primer 73tgccaattag ccacactctt c 217420DNAArtificial SequencePCR primer 74gtgcaaagtg ccttacacag 207521DNAArtificial SequencePCR primer 75gaggttagac gggtctgagt t 217621DNAArtificial SequencePCR primer 76tggcaggcag tagtaactct g 217720DNAArtificial SequencePCR primer 77ctgggattac aggtgtctgg 207821DNAArtificial SequencePCR primer 78tgagctgttc ccacaccaca t 217921DNAArtificial SequencePCR primer 79tcaggacgtt gcactttgac a 218021DNAArtificial SequencePCR primer 80gaatgctgga tgtggattga g 218120DNAArtificial SequencePCR primer 81gagcagaagg ccttgactga 208221DNAArtificial SequencePCR primer 82gccttcagtt tgtcccgttc t 218320DNAArtificial SequencePCR primer 83cgcgaggtca ggagttcgat 208421DNAArtificial SequencePCR primer 84ccactgaaca gggcacaaga t 218521DNAArtificial SequencePCR primer 85gaattactgg ctgaggcaac c 218617DNAArtificial SequencePCR primer 86gcctgcgctt acatagt 178721DNAArtificial SequencePCR primer 87aggcgggcgg atcacaaggt c 218822DNAArtificial SequencePCR primer 88ggctcactgc aacctccacc ta 228924DNAArtificial SequencePCR primer 89aaccaaacaa gccagcaatc actc 249022DNAArtificial SequencePCR primer 90tgggaggctg aggcaagaga at 229118DNAArtificial SequencePCR primer 91actgggcctg ggatgtat 189219DNAArtificial SequencePCR primer 92aaaaattggc tgggtgtgg 199322DNAArtificial SequencePCR primer 93caggctggag tgcaatggtg tg 229422DNAArtificial SequencePCR primer 94tgggaggctg aggcaagaga at 229520DNAArtificial SequencePCR primer 95ctcactgcag cctcaactcg 209624DNAArtificial SequencePCR primer 96tagcagaagg gggaaggggg aacg 249724DNAArtificial SequencePCR primer 97gacggtgtac acatttggtt agtt 249824DNAArtificial SequencePCR primer 98gcacagttac atccgcctta tcgt 249924DNAArtificial SequencePCR primer 99tgcagacacc cagagaagac gact 2410022DNAArtificial SequencePCR primer 100tggaccctgc cctcacgatg ga 2210156DNAHomo sapiens 101tgggattaca ggtgcatgct accatgcccg gctaattttt ttgtattttt ttagta 5610216DNAHomo sapiens 102accatgcccg gctaat 1610321DNAHomo sapiens 103ctaccatgcc cggctaattt t 2110421DNAArtificial SequencePCR primer 104tactactggg cctgggatgt a 2110522DNAArtificial SequencePCR primer 105tagagaaaga cctcgttatt gg 22

Claims

1-40. (canceled)

41. A method for regulating expression of a SOST gene, comprising expressing a SOST protein product of a SOST coding sequence in the presence of at least one SOST gene regulatory sequence, wherein the SOST gene regulatory sequence comprises a polynucleotide which comprises a nucleic acid sequence of SEQ ID NO:2, or a complementary sequence thereto.

42. The method of claim 41 wherein the at least one SOST gene regulatory sequence is comprised within genomic DNA.

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