Regeneration

Abstract

the field of regeneration of cells and the vegetative propagation of (micro)-organisms or specific parts such as tissues or organs thereof, for example of those cells grown in tissue or organ culture, and more in particular to the seedless propagation of plants. The invention provides a culture method for propagation of a plant from plant starting material wherein during regeneration of said starting material, especially in the phase of the development of the shoot-root body plan, root or shoot initiation is stimulated by a recombinant gene product or functional fragment thereof, for example derived from a gene involved in the regulation of plant development allowing reducing or omitting exogenous phytohormone addition to said culture.

Description

[0001]The invention relates to the field of regeneration of cells, self-renewal of (micro)-organisms, the vegetative propagation of plant parts such as plant tissues or organs thereof, for example cells grown in tissue or organ culture, and more in particular to the seedless propagation of plants.

[0002]Renewal of plant and animal cells into more cells, tissues, organs and even whole plants and organisms is a process central to life that has been set to men's whims and desires already for a long time. Self-renewal of specific micro-organism starter cultures are used to ferment foods and drinks. Yet other cultures are useful for the metabolites they produce per se, such as produced by modern day's large scale fermentor cultures for the production of antibiotics or enzymes. Within the realm of animal cells, use of the renewed cultured cells, although being of fairly recent date, has taken great flight with the production of for example viral vaccines in cell- or tissue culture. Even more recent is the use of donor cells harvested from an individual, and grown and/or differentiated in culture, for transplantation purposes. Such cells (take for example bone marrow cells) are, after having been sufficiently regenerated and differentiated, proliferated or equipped with the desired characteristics, transplanted into a recipient for medical purposes. Shortly, such therapies will even include transgenic cells, transformed with modern recombinant techniques, that are thereby equipped with the desired characteristics and transplanted.

[0003]Regeneration is very well studied in plants, where it is crucial in vegetative propagation. In principle, plants can be propagated in two ways, via seeds or vegetatively without using seeds as starting material to obtain the desired plant. Both types of propagation may be impossible or undesirable under certain conditions. When propagation via seeds is unsatisfactory (when no seeds or too few of the desired seeds are formed or the desired seeds quickly loose their germination viability) then seedless propagation is often adopted. Also, when due to sexually crossing a very heterogenous progeny is or may be obtained due to its strong heterozygosity, propagation via seeds is often also considered unsatisfactory. Of course, seedless propagation of essentially seedless starting material may in a later phase give rise to the desired seeds, which can further be used to obtain the desired plants.

[0004]Within seedless propagation of plants two major fields can be distinguished: In vivo and in vitro vegetative propagation. In vivo vegetative propagation (via for example cuttings, splitting or division, layering, earthing up, grafting or budding, and other methods known to the gardener or horticulturist), has for many years played an important role in agriculture; e.g. with potatoes, apples, pears, many ornamental bulbs and tuberous plants like potatoes, many arboricultural crops, carnations, chrysanthemums, etc. Vegetative propagation is also very important in plant breeding: parent lines have to be maintained and propagated vegetatively for seed production; cloning is often required for setting up gene banks; adventitious shoot formation is needed to obtain solid mutants after mutation induction.

[0005]However, the classical methods of in vivo vegetative propagation often fall short (to slow, too difficult or too expensive) of that required or are completely impossible. In the last couple of decades, since the discovery that plants can be more rapidly cloned in vitro than in vivo, knowledge concerning vegetative propagation has grown quickly; this holds equally true for plants from temperate, subtropical as well as tropical regions. It has now even become possible to clone species by in vitro culture techniques that are impossible to clone in vivo. Different methods of in vitro vegetative or seedless propagation from plant starting material are for example using single-node cuttings, axillary branching, regeneration of adventitious organs (roots or shoots) on starting material such as explants or callus tissue and regeneration of plants from suspensions of, or even single, cells or protoplasts used as starting material. For the generation of transformed or transgenic plants, in vitro propagation is even considered a prerequisite, since it is the totipotency of individual plant cells that underlies most plant transformation systems.

[0006]To propagate plants from starting material in vitro, it is in principle necessary that at least one cell in the starting material is capable of regeneration. The ability to regenerate is for example determined by the genotype, the environmental conditions (nutrient supply, regulators and physical conditions) or the developmental stage of the plant, or combinations of these. It is well known that some families and genera have high regeneration ability: Solanacea (Solanum, Nicotiana, Petunia, Datura, and Lycopersion), Crucifera (Lunaria, Brassica, Arabidopsis), Generiaceae (Achimenes, Saintpaulia, Streptocarpus) Compositae (Chicorium, Lactuca, Chrysantemum), Liliaceae (Lilium, Haworthia) Allium, Ornithogalum) but others, such as many decorative plants, woody species such as shrubs, conifers or trees, especially fruit trees, Rosacea, Alstroemeria, Euphorbia, and bulbs such as Tulipa, and others are notoriously difficult, even with in vitro techniques.

[0007]As indicated above, regeneration (self-renewal of (micro-)organisms and self-renewal of plants, animals or parts thereof, i.e. vegetative reproduction/propagation) can also be considered a repair strategy observed throughout the realm of micro-organisms, animal and plant species. Regeneration in plants for example comprises the formation of new tissues containing both root and shoot meristems, separate shoot or root meristems, plant organs or organ primordia from individual cells or groups of cells. Regeneration in general mimics the process of normal cellular and organ differentiation that takes place during plant development and results in the formation of the different plant organs. In normal development, early in ontogony, cells and tissues of common lineage diverge into often contrasting paths of development as they respond to developmental signals. This ability to develop in response to a specific signal is also known as cellular competence or cellular potentiality. As competent cells become committed to particular paths of differentiation, they are not readily diverted into other pathways; this restriction of the developmental potentiality of cells is referred to as determination.

[0008]Plant cells or groups of cells that under normal conditions are unable to initiate the formation of certain plant organs, meristems or organ primordia can often be stimulated by extracellular stimuli modifying the differentiation stage of the cell. Extracellular diffusible factors have shown to be essential for cellular redifferentiation in plant cells (Siegel and Verbeke, 1989 Science 244, 580-582). The perception of these signals at the cellular surface and the intracellular signal transduction that finally result in changes in transcriptional regulation provides cells with the ability to respond to such extracellular stimuli. Regeneration can result in the formation of either a shoot alone or a root alone or both together. Only after redifferentiation of a cell or tissue, regeneration is possible that results in differentiated tissue that again comprises the necessary three-dimensional layout of the emerging plant, the apical-basal or shoot-root body plan from which the mature desired plant can develop.

[0009]Indeed, central in in vitro techniques for seedless propagation are phytohormones and other factors often added to the culture medium that mimic these extracellular stimuli. For the process of regeneration of the original starting cell into a multicellular totipotent tissue underlying and preceding somatic embryogenesis or organogenesis in vitro in cell, tissue or explant cultures which lead to a fully differentiated plant again, in general a well balanced, and per plant species often different, phytohormone addition to the culture is required. Overall, a balance is required between auxins on the one hand and cytokinin on the other. After exogenous exposure to auxin (such as 2,4-dichlorophenoxyacetic acid (2,4-D), chloramben or dicamba) or cytokinin (such as 6-benzylaminopurine or zeatine) or both, cells or tissue react by development of the shoot-root body plan, for example by forming shoots and/or roots, sometimes readily, sometimes erratically especially when the proper balance between the hormones is not properly selected.

[0010]Regeneration in vitro and especially the manipulatable nature of in vitro culture thus depends mainly on the application of these two types of hormones, and also on the ability of the tissue to respond to phytohormonal changes during culture. In general, three phases of regeneration are recognisable. In the first phase, cells in the culture acquire "competence", which is defined as the ability (not capacity) to respond to hormonal signals of organ induction. The process of acquisition of said organogenic competence is often referred to as "dedifferentiation" of differentiated cells to acquire organogenic competence. The competent cells in the culture are canalised and determined for specific tissue and organ formation for re-entry of quiescent cells into cell cycle, and organisation of cell division along the lines of the shoot-root body plan to form specific primordia and meristems under the influence of the phytohormone balance through the second phase. Especially auxin is thought to be involved in specific regenerative signal transduction pathways for adventitious root initiation, whereas cytokinin is thought to be involved in specific regenerative signal transduction pathways for adventitious shoot initiation.

[0011]Then the morphogenesis, the growing of the plant to its fully differentiated state, proceeds independently of the exogenously supplied hormones during the third phase.

[0012]Although the general principles governing regeneration via addition of exogenous phytohormones are thus fairly well understood, designing working in vitro culture protocols finding the right balance, the right time of administration or the right type or subtype of said hormones for a great many individual species is still more or less a process of trial-and-error. However, as already indicated above, for in vitro regeneration or seedless propagation of a great many plant species is a large interest, especially for those that are in general hard to propagate.

[0013]The invention provides a culture method for propagation of a plant from plant starting material wherein, especially in the phase of the development of the shoot-root body plan, root or shoot initiation is stimulated by introducing at least one recombinant gene product or functional fragment thereof in said starting material, for example by stimulating at least one signal transduction pathway for root or shoot initiation, said gene product or gene products for example derived from a gene or genes involved in the regulation of plant development, allowing reducing or omitting exogenous phytohormone addition to said culture in the regeneration process. In a preferred embodiment the invention provides a culture method for vegetative propagation of plants from plant starting material comprising regeneration of said starting material wherein during regeneration of said starting material at least one specific signal transduction pathway for adventitious root or shoot initiation is endogenously stimulated allowing reducing or omitting exogenous phytohormone addition to said culture, in particular wherein said pathway is endogenously stimulated by a recombinant gene product derived from a gene involved in the developmental regulation of regeneration, such as a gene or gene product involved in hormone production, a gene or gene product giving feed back on hormone production, or involved in the cascade of events leading to regeneration.

[0014]Preferably, the method as provided by the invention comprises at least one step of in vitro culture, since it is in in vitro culture that the auxins or cytokinins are most widely used, in the regeneration process, especially for plants that are notoriously difficult to regenerate for vegetative propagation such as many decorative plants, woody species such as shrubs, conifers or trees, especially fruit trees, Rosacea, Alstroemeria, Euphorbia, and bulbs such as Tulipa. However, clearly, said hormones are also commonly used in in vivo cultures as well, (in vivo cultures essentially being all crop or plant culture methods traditionally used in agriculture) where such hormones are commonly added by (root or stem) dipping, spraying or watering. Especially those plants that are propagated in an essential seedless way can now be regenerated or propagated more easily, consequently, in a preferred embodiment, the invention provides a culture method for essentially seedless propagation of plants from plant starting material comprising regeneration of said starting material wherein during regeneration at least one specific signal transduction pathway for adventitious root or shoot initiation endogenously is stimulated, e.g. by above mentioned gene product, allowing reducing or omitting exogenous phytohormone addition to said culture.

[0015]Essentially seedless propagation herein is defined in that said starting material essentially comprises no seeds, or at least that seed possibly present in said starting material does not lay at the basis of the regeneration of said starting material or does not develop into the desired plant. However, as one aspect of the culture method comprising regeneration as provided by the invention, during or after the process of regeneration or propagation according to the invention seed may be formed, from which even a desired plant may develop, which is a result of the propagation according to the invention, rather than that it lays at the basis thereof.

[0016]In particular, the invention provides a culture method wherein said starting material comprises an individual plant cell or protoplast or explant or plant tissue, materials which are commonly used in in vitro culture methods whereby the addition of phytohormones was thought to be axiomatic. Now such addition is no longer necessary or can be reduced, providing an easier way of in vitro culture, wherein not such an intricate balance between the addition of the various hormones has to be sought.

[0017]The invention provides manipulation of propagation characteristics of for example plant tissue. Numerous plant species are propagated in tissue culture in order to obtain large amounts in a relative short period of time. Using the invention it is relatively easy to increase the multiplication factor several times. For several notoriously difficult species, like shrubs, trees en various bulbous species it is now also possible to use essentially seedless propagation, and especially in vitro culture, when using the invention. The regeneration capacity of cells or tissue isolated from these plants is increased significantly, thereby increasing the multiplication factor by introducing of certain bioactive molecules, like nucleic acid or (modified) protein. The nucleic acids or proteins may be introduced by the methods known in art, like particle gun bombardment, electroporation, micro-injection or other techniques described in the introduction. The introduced molecules are either nucleic acid, being RNA, or naked DNA with a small chance of becoming integrated in the genome, or (modified) protein product. The molecules will in general be lost during the regeneration process and are therefore only transiently present. The nucleic acids that may be used encode or produce proteins that stimulate the regeneration process and reduce or eliminate the use of exogenously added planthormones. The proteins that may be added are the protein products of these nucleic acids or their modified forms. Examples of molecules with the above described characteristics are proteins or genes coding for proteins involved in the regulation of plant development or perception of plant hormones. By using the invention the multiplication factor can be increased so much that it will be possible to use in vitro propagation techniques in a broader sense and also for the more difficult species, Also, by using the invention it is relatively easy to permanently increase the propagation characteristics for these plants. The regeneration capacity of these plants can be increased significantly if these plants are made transgenic by introducing a gene coding for proteins involved in the regulation of plant development or perception of plant hormones or more specific a gene coding for a product stimulating or inducing one signal transduction pathway for root or shoot initiation or even more specific a gene coding for a representative of the plant receptor kinase family RKS. Transformation can be achieved using the techniques known in the field like Agrobacterium mediated transformation, particle gun bombardment, the above described marker-free transformation system or others and select for non-lethal expressors of the gene.

[0018]In one preferred embodiment, the invention provides a culture method according to the invention wherein said starting material comprises a desired somatic mutation. Mutations can occur in any cell of a living organism, but are only transferred to the offspring when this mutation occurred in those cells from which gametophytic cells of that organism are derived. Somatic mutations are usually lost unless the tissue in which the mutation is apparent is vegetatively propagated or if cells in this tissue are regenerated to form an intact new organism. Using the technology described in this invention the rescue of somatic mutations in plants is provided. Somatic, but also generative tissue is stimulated to regenerate by the introduction of bioactive molecules, like nucleic acid or (modified) protein as provided by the invention. The nucleic acids or proteins may be introduced by the methods known in art, like particle gun bombardment, electroporation, micro-injection or other techniques described. The introduced molecules are either nucleic acid, being RNA, or naked DNA with a (not necessarily) small chance of becoming integrated in the genome, or (modified) protein product. The molecules will in general be lost during the regeneration process and are therefore in general only transiently present. The nucleic acids that may be used encode proteins that stimulate the regeneration process and reduce or eliminate the use of exogenously added planthormones. The proteins that may be added are the protein products of these nucleic acids or their modified forms. Examples of molecules with the above described characteristics are proteins or genes coding for proteins involved in the regulation of plant development or perception of plant hormones. Alternatively somatic mutations may have been created by treatment of seeds with mutagenic agents, like colchicines, EMS, radiation or carcinogenic substances etc. The sectors in these mosaic plants grown from these treated seeds will be screened for desirable phenotypes. The interesting sectors will subsequently be isolated and used as starting material for regeneration by the above-described invention in order to obtain clonal propagation of these desired traits.

[0019]In another preferred embodiment, the invention provides a culture method according to the invention wherein said starting material comprises transgenic material. These days transgenic plants are being produced rapidly, albeit often in only limited numbers. To rapidly acquire sufficient numbers of plants for further propagation under field conditions, in vitro culture techniques are widely used. The invention now provides a method wherein little or no attention has to be given to phytohormone levels in such transgenic plants cultures.

[0020]In particular, the invention provided a method wherein said starting material additionally comprises starting material comprising a recombinant nucleic acid encoding a desired trait. The invention herewith provides essentially marker-free transformation, or at least it provides plants that after transformation and propagation are essentially marker-free. A recombinant nucleic acid encoding a desired trait, that one would like to integrate in a plant's genome is provided to at least part of said starting material with gene delivery vehicles or methods, such as vectors, particle bombardment, electroporation, micro-injection or other techniques described in the art. Cells comprising said recombinant nucleic acid are also provided according to the invention with at least one recombinant gene product or functional fragment thereof, for example by stimulating at least one signal transduction pathway for root or shoot initiation, said gene product or gene products for example derived from a gene or genes involved in the regulation of plant development, allowing reducing or omitting exogenous phytohormone addition to said culture. In particular, the invention provides a culture method for vegetative propagation of plants from plant starting material having been provided with a recombinant nucleic acid encoding a desired trait comprising regeneration of said starting material wherein during regeneration of said starting material at least one specific signal transduction pathway for adventitious root or shoot initiation is endogenously stimulated allowing reducing or omitting exogenous phytohormone addition to said culture, in particular wherein said pathway is endogenously stimulated by a recombinant gene product derived from a gene involved in the developmental regulation of regeneration, such as a gene or gene product involved in hormone production, a gene or gene product giving feed back on hormone production, or involved in the cascade of events leading to regeneration.

[0021]In a preferred embodiment, said recombinant nucleic acid encoding a desired trait has additionally been provided with means for nuclear targeting and/or integration in a plant genome. Such means can be nucleic acid signals incorporated with the recombinant nucleic acid encoding the desired trait, or proteinaceous substances such as transposases, or viral or bacterial proteins (such as Vir-proteins) to protect the recombinant nucleic acid inside the cell, taking care of proper targeting towards the nucleus and/or stimulating proper integration.

[0022]Even more preferred, the invention provides a method wherein said starting material comprises a to be transformed individual plant cell or protoplast or explant or plant tissue comprising recombinant nucleic acid encoding a desired trait among other, non-transformed starting material from which the transformed material has to be selected.

[0023]In general, as a part of the process of for example plant transformation, dominant selectable markers are used to select transgenic cells from which transgenic plants can be regenerated. For one thing, these marker genes are generally superfluous once an intact transgenic plant has been established. Furthermore, selectable marker genes conferring for example antibiotic or herbicide resistance, used to introduce economically valuable genes into crop plants have major problems: detoxification of the selective agent by expression of a modifying enzyme can enable untransformed cells to escape, dying untransformed cells release products which are toxic and inhibit the regeneration of transformed cells, the selective agents may have negative effects on proliferation and differentiation of cells, there is uncertainty regarding the environmental impact of many selectable genes, and it is difficult to perform recurrent transformations using the same selectable marker to pyramid desirable genes. The invention now provides a method reducing or omitting selective agent addition to said culture.

[0024]Attempts have been made earlier to design transformation systems allowing marker gene elimination to obtain marker-free transformants of diverse plant species whereby the marker gene used is removed from the transformed cell after it has performed its task. One method involves co-transformation of cells mediated by Agrobacterium tumefaciens with binary vectors carrying two separate T-DNAs, one for example comprising a drug-resistance selection marker gene, another comprising the desired gene, followed by conventional out-breeding the undesired drug-resistance gene, that is thought to localise at a different locus than the desired gene. Although drug sensitive transformants comprising the desired gene may be thus obtained it is not clear whether all these transformants are indeed totally free of (non or partly functional) selection marker-gene or fragments thereof. Also, the selective agent initially used still has the unwanted negative effects on proliferation and differentiation of plant cell during the transformation process. Furthermore, the method requires sexual crossing which limits it to plant species where sexual crossing, and not vegetative reproduction, is the practical method of reproduction, and practically limits it even further to those plant species with a sufficient short generation time.

[0025]One strategy currently available to eliminate the superfluous marker after the cell has been transformed without the need to sexually cross plants is the MAT vector system. However, said system relies on intrinsic post-transformational excision of the selection gene which is comprised in a transposable element, an event which only haphazardly occurs and reduces the final efficiency of the transformation process.

[0026]Yet another strategy involves site specific recombination such as seen with the Cre-Lox system whereby in a first transformation the selection-marker gene is inserted at a previously determined specific site, allowing selection of transformed cells, after which in a second transformation comprising the introduction of a site specific recombinase, the selection-marker gene is again excised from the genome.

[0027]Needles to say that, apart from other problems, the prerequisite of having a suitable site in the to be transformed cell available restricts said method to those organisms of which the genome is well known. The invention now provides a method to obtain transformed plants by in vitro culture wherein said transgenic material is devoid of a selectable marker gene conferring resistance to an selective agent. Resistance to selective agents is no longer needed since according to the invention the transformed material is equipped with the necessary recombinant gene product or gene products or functional fragment(s) thereof derived from a gene involved in the regulation of plant development allowing reducing or omitting exogenous phytohormone addition to said culture, thereby giving preferred growth conditions to the transformed cells over those non-transformed cells that have not been provided with said gene product or functional fragment thereof. In particular, the invention provides a culture method for vegetative propagation of plants from transformed plant starting material comprising regeneration of said starting material wherein during regeneration of said transformed starting material at least one specific signal transduction pathway for adventitious root or shoot initiation is endogenously stimulated allowing reducing or omitting exogenous phytohormone addition to said culture, in particular wherein said pathway is endogenously stimulated by a recombinant gene product derived from a gene involved in the developmental regulation of regeneration. The beauty of it is that no selectable marker gene conferring resistance to a selective agent has to be introduced in said material at all, thereby obviating the need to deplete the transformed material of such marker genes afterwards. In particular, the invention thus does not make use of resistance to antibiotic or herbicides, and does nor carry all the disadvantages associated herewith.

[0028]In short, most plant transformation systems are based on the selection for herbicide or antibiotic resistance or selection for transformants is based on the presence of an additional selection marker besides the trait itself. Using the technology described in this invention, markerless transformation in plants is provided. This new transformation/regeneration (t/r) system for example consist of two components (FIG. 20). A first component in this example is the trait, which may be present between the borders of Agrobacterial T-DNA, but apart from a suitable promoter no other DNA is needed. This first component may be single or double stranded DNA and may be in vitro coated with the VirE2 protein and/or a molecule of VirD2 (preferentially covalently attached to the 5'-end of this DNA). The Vir-proteins may be present to protect the DNA inside the plant cell, take care of proper targeting towards the nucleus and will stimulate proper integration into plant DNA. Tissue will be stimulated to regenerate by the introduction of certain bioactive molecules. These bioactive molecules act as the second component. The second component is either nucleic acid, being RNA, or naked DNA with a small chance of becoming integrated in the genome, or (modified) protein product.

[0029]The nucleic acids or proteins (second component) may be introduced mixed with the first component by the methods known in art, like particle gun bombardment, electroporation, micro-injection or other techniques described in the introduction. Both components have to be present in the plant cell together in sufficient quantities, but the ratio between the two components may vary depending on the species and the preferred number of integration's of the trait in the plant DNA. The second component will preferably be lost during the regeneration process and is therefore only transiently present, whereas the first component has a high change of becoming integrated into the plant genome. The second component is a nucleic acid or a mixture of nucleic acids that will produce proteins that stimulate the regeneration process and reduce or eliminate the use of exogenously added planthormones or is the protein product or a mixture of products of these nucleic acids or their modified forms or a mixture of both. Examples of molecules with the above described characteristics are proteins, or genes coding for proteins involved in the regulation of plant development or perception of plant hormones. The main advantages of the this t/r-system are, as explained with the example of FIG. 20: [0030]only the trait is introduced into the plant DNA; apart from the T-DNA borders (Only in the case when VIR proteins are used, it is necessary to include T-DNA borders onto the trait DNA), if present, no other unwanted DNA, like a selection marker, is present. In order to allow the process of homologous recombination of the trait DNA into the corresponding endogenous DNA on the plant genome, genes or gene products encoding At R51, AtRAD51 or RecA or gene products with similar function can be applied in the second component in order to result in transient expression of the recombinase. After targeting and localized integration of the trait DNA, the recombinase is lost. [0031]the principle of regeneration is universally applicable [0032]the amount of exogenous plant hormones for regeneration can be reduced or omittedactive selection is not necessary as mainly transformed cells will regenerate.

[0033]Said gene involved in the regulation of plant development can be selected from a great many genes already known, or yet to be determined, to be involved in regeneration. Examples of such genes are clavata (Clark et al., 1997, Cell 89, 575-585) and primordia timing genes (Mordhorst et al, 1998 Genetics 149, 549-563), which are stimulating regeneration when inactivated, Leafy-Cotelydon gene (LEC, Lotan et al., 1998, Cell 93, 1195-1205), the KAPP gene (Stone et al., 1994, Science 266, 793-795; Stone et al., 1998, Plant Physiol. 117, 1217-1225), IPT (Morris, R. O., 1986 Annu. Rev. Plant Physiol. 37, 509-538), WUSCHEL (Mayer et al. 1998 Cell 95, 805-815; Schoof et al. 2000 Cell 100, 635-644), KNAT1&2 (the Arabidopsis kn1-like gene) (Chuck et al. 1996. Plant Cell 8, 1277-1289; Lincoln et al. 1994 The Plant Cell 6, 1859-1876), SHOOT MERISTEMLESS gene (Endrizzi et al. 1996 Plant J. 10, 967-979), CUP-SHAPED COTYLEDON (Aida et al. 1999 Development 126, 1563-1570), CYCLIN D (Cockcroft et al. 2000 Nature 405, 575-579; Riou-Khamlichi et al. 1999 Science 283, 1541-1544), CKI1 (Kakimoto 1996 Science 274, 982-985), AINTEGUMENTA (Mizukami and Fischer 2000 PNAS 97, 942-947; Krizek 1999 Dev. Genetics 25, 224-236), SBP-box proteins (Cardon et al. 1999 Gene 237, 91-104), CDC2a (Hemerly et al. 1993 The Plant Cell 5, 1711-1723), which are genes that stimulate regeneration when induced or overexpressed, or antagonists thereof or others that are involved in the regulation of plant development in the broadest sense, such as can be found by studying plant embryogenesis or organogenesis on the molecular level. In particular, a population of gene products involved in regeneration is represented by the intracellular signal transduction factors that are directly phosphorylated by RKS protein and thereby activated.

[0034]In a preferred embodiment, the invention provides a method according to the invention wherein said gene involved in the regulation of plant development encodes a leucine-rich repeat containing receptor-like kinase, such as present in plant database collections, with homology to the extracellular domain of the Arabidopsis RKS protein family, such as:

GB:AW011134 AW011134 ST17B03 Pinus taeda GB:LELRPGENE X95269 L. esculentum GB:AI775448 AI775448 EST256548 Lycopersicon esculentum GB:AI496325 AI496325 sb05c09.y1 Gm-c1004 GlycineGB:AI487272 AI487272 EST245594 Lycopersicon esculentum GB:AI441759 AI441759 sa82d08.y1 Gm-c1004 Glycine max GB:AI782010 AI782010 EST262889 Lycopersicon esculentum GB:AI772079 AI772079 EST253179 Lycopersicon esculentum GB:SBU62279 U62279 Sorghum bicolor GB:C22645 C22645 C22645 Oryza sativa GB:D49016 D49016 RICS15625A Oryza sativa GB:AI776399 AI776399 EST257499 Lycopersicon esculentum GB:AI776208 AI776208 EST257308 Lycopersicon esculentum GB:AI352795 AI352795 MB61-10D PZ204.BNlib Brassica napus GB:AQ578072 AQ578072 nbxb0092C18f Oryza sativa GB:C95313 C95313 C95313 Citrus unshiu MiyagawaGB:AI162893 AI162893 A026P38U Hybrid aspenGB:AI782076 AI782076 EST262955 Lycopersicon esculentum

GB:AI726177 AI726177 BNLGHi5165 Cotton

[0035]GB:AI777982 AI777982 EST258861 Lycopersicon esculentum GB:AI774881 AI774881 EST255981 Lycopersicon esculentum GB:AI896737 AI896737 EST266180 Lycopersicon esculentum GB:AI676939 AI676939 605047A07.x1 Zea mays GB:D40598 D40598 RICS2674A Oryza sativa GB:OSU82168 U82168 Oryza sativa GB:SBRLK1 Y14600 Sorghum bicolor GB:AI495359 AI495359 sa97a09.y1 Gm-c1004 Glycine max GB:C96041 C96041 C96041 Marchantia polymorpha, or such as present in plant database collections, with homology to the intracellular domain of the Arabidopsis RKS protein family, such as:GB:AI896277 AI896277 EST265720 Lycopersicon esculentum GB:AU056335 AU056335 AU056335 Oryza sativa GB:AA738546 AA738546 SbRLK4 Sorghum bicolor GB:AA738544 AA738544 SbRLK2 Sorghum bicolor GB:AA738645 AA738545 SbRLK3 Sorghum bicolor GB:SBRLK1 Y14600 Sorghum bicolor GB:AI7290900 AI729090 Gossypium hirsutum GB:AI920205 AI920205 Pinus taeda GB:AI896183. AI896183 EST265626 Lycopersicon esculentum GB:AI967314 AI967314 Lotus japonicus GB:AI730535 AI130535 BNLGHi7007 Gossypium hirsutum GB:AF078082 AF078082 Phaseolus vulgaris GB:CRPK1 Z73295 C. roseus GB:C22536 C22536 C22536 Oryza sativa GB:C22530 C22530 C22530 Oryza sativa GB:CMA010166 AJ010166 Zea mays mRNAGB:AQ271213 AQ271213 Oryza sativa, or known from Schmidt et al (1997, Development 124, 2049-2062, WO 97/43427), where for example stable transformation, but not regeneration, nor transient expression nor use in selection, of plants with SERK (RKS0) is considered. Also applicable in a method according to the invention are bacterial genes or fragments thereof such as the AK-6b gene (Wabiko et al, Plant Physiol. 1996, 939-951) or the rolABC genes (Jasik J, Plaint Science, 1997, 57-68), however, where only regeneration by stable transformation is intended, plant genes such as those disclosed herein are preferred.

[0036]In a preferred embodiment, the invention provides a method according to the invention wherein said gene involved in the regulation of plant development encodes a leucine-rich repeat containing receptor-like kinase, wherein said receptor-like kinase is a representative of a plant receptor kinase family RKS such as shown in FIG. 3.

[0037]In particular, the invention provides a method wherein said gene product or functional fragment thereof is derived from a receptor-like kinase that comprises an N-terminal signal sequence, an extracellular region comprising a leucine zipper domain, a disulphate bridge domain, a leucine rich repeat domain comprising 3-5 leucine rich repeats, a transmembrane domain, an intracellular region comprising an anchor domain, a serine/threonine kinase domain and/or a C-terminal leucine rich repeat domain.

[0038]These genes encode membrane spanning proteins having a particular function in signal transduction, thereby being prime candidate genes to provide gene products or functional fragments thereof to be employed in a method of the current invention.

[0039]In particular, the invention provides a method wherein said receptor-like kinase is encoded by a nucleic acid which in Arabidopsis thaliana comprises a sequence as shown in anyone of FIG. 4 or 8 to 20. Suitable receptor kinase-like genes from plants other than Arabidopsis thaliana, such as Daucus carota, Rosa, Gerbera, Chrysanthemum, Alstroumeria, Lilium, Tulipa, Dyanthus, Cymbidium, Gypsopays, Ficus, Calangoe, Begonia, Phalasnopsis, Rhonondendrum, Spatiphilus, Cucubitaceae, Solanaceae, and grasses such as cereals are easily found using the Arabidopsis thaliana sequences provided herein by methods known in the art. In general for each RKS gene identified in Arabidopsis thaliana a corresponding RKS gene is present in individual species of both monocotyledon as well as in dicotyledon plants. The invention provides a method wherein said receptor-like kinase is encoded by a plant derived nucleic acid corresponding or homologous to a nucleic acid which in Arabidopsis thaliana comprises a sequence as shown in anyone of FIG. 4 or 8 to 20. Corresponding or homologous RKS genes and gene products in plant species other than Arabidopsis thaliana are isolated by various approaches. For example by screening of cDNA and genomic libraries using Arabidopsis RKS cDNA probes under low stringency hybridisation/washing conditions as described above, alternatively by the use of degenerated RKS primers (for example primer combination RKS B forward and RKS E reverse as shown herein in order to amplify an exon fragment of the desired gene. Full length cDNA clones can further be obtained by race and tail PCR approaches. Also, the generation of antibodies recognising conserved or distinct and specific regions within different members of RKS gene family within a plant species allow the desired isolation. Alternatively, specific antibodies are generated that recognise one specific RKS gene product in a variety of plant species. These antibodies are used to screen cDNA expression libraries of plant species. Furthermore, it is possible to screen for RKS-homologous sequences in electronic databases. Searches are performed both on nucleotide and on amino acid level. Additionally, RKS genes and gene products in plant species other than Arabidopsis thaliana are isolated for example by two or three hybrid screenings in yeast with RKS clones in other to isolate (hetero) dimerizing members of this RKS family in similar or unrelated plant species.

[0040]In one embodiment, the invention provides a method for propagation of a plant from plant starting material wherein during regeneration of said starting material at least one signal transduction pathway for root or shoot initiation is stimulated by a recombinant gene product or functional fragment thereof derived from a gene involved in the regulation of plant development allowing reducing or omitting exogenous phytohormone addition to said culture, wherein said gene product or functional fragment thereof is introduced in at least a part of the starting material by transformation. The invention also provides the introduction of regenerating gene constructs into cells which can lead to the regeneration of the cell itself or to the induction of regeneration processes in neighbouring cells, even somatic embryos resulting from said induced cells are provided herewith. Individual transformed cells are generated that are essential for the differentiation state of surrounding cells. Introduction of such an inducing regenerator as provided herewith into plant cells results in the formation of a proliferation of neighbouring cells and the formation of new plants or parts thereof from these proliferating cell masses. The originally transformed plant is not necessarily included in the proliferation process itself an is therefore not necessarily part in the resulting regenerating plants or parts thereof. This specific from of induced regeneration of neighbouring cells provide herewith gives the option to regenerate plants that do not contain the introduced gene or gene product, and therefore represents a method to induce regeneration without the necessity to introduce gene products into an originating cell population and having to maintain these gene products or nucleic acids encoding therefore. An example of the process of induced induction is shown in FIG. 6F, where a single GUS positive cell marks the original introduction site for the bombarded DNA constructs. Above this cell, a proliferating cell mass has been formed that is clearly GUS negative. On top of this induced proliferated cell mass, we could detect several structures that morphologically represent somatic embryos. These somatic embryos develop from the borders of the proliferating cell mass as previously described (Schmidt et al. 1997, Development 124, 12049-2062). Somatic embryos provide an excellent source of regenerating plant since all the organs and plant parts are formed by similar processes as take place during zygotic embryogenesis. This observation clearly indicates the potential of this class of regenerating molecules to induce a proliferating, non-transformed cell mass from which new plantlets can be regenerated. It provides the means to induce somatic embryos directly on living plant tissues, even without the prior need to introduce an in vitro culture procedure.

[0041]Again, transformation as provided here can be thus either in a stable fashion where the introduced genetic information or nucleic acid is integrated into the nuclear, chloroplast or mitochondrial genome, and is either constitutively or inducibly expressed but preferably is transient, wherein the nucleic acid is not introduced into the genome and gets lost after a certain period after introduction. Transformation of recombinant DNA or RNA into the cell or protoplast can take place in various ways using protocols known in the art, such as by particle bombardment, micro-injection, Agrobacterium-mediated transformation, viral-mediated transformation, bacterial conjugation, electroporation, osmotic shock, vesicle transport or by direct gene transfer, with or without the addition of a proteinaceous substance bound to the nucleic acid molecule. Integration of a proteinaceous substance into cells or protoplast can be facilitated along the lines of the transformation protocols as described above. A cell or protoplast thus having been provided with a gene product (i.e. a DNA, RNA or proteinaceous substance or functional fragment thereof) derived from a gene involved in the regulation of plant development can now regenerate on its own, allowing reducing or omitting exogenous phytohormone addition to the culture that comprises that cell or protoplast. The process of vegetative propagation is hereby very much simplified, large numbers of plants with an identical genetic background can now be obtained staring from starting material with the desired characteristics.

[0042]In a preferred embodiment, the present invention provides a method for propagation of a plant from plant starting material wherein said starting material comprises a cell or protoplast transformed with a desired nucleic acid sequence intended to provide the resulting transgenic plant arising from that cell or protoplast with desirable characteristics. Such a cell or protoplast, according to the invention having been provided with a gene product (i.e. a DNA, RNA or proteinaceous substance or functional fragment thereof), for example derived from a gene involved in the regulation of plant development can now regenerate on its own, allowing reducing or omitting exogenous phytohormone addition to the culture that comprises that transformed cell or protoplast. Selection for regenerating cells or tissues after the transformation of the desired sequence together with the regenerating gene product results in the recovery of only those plants or plant material that contain the desired nucleic acid sequence, preferably integrated in a stable fashion in the plant's genome, and the regenerating gene product, thereby providing a selection of the desired transgenic plant based on the selective regeneration of the transformed starting material.

[0043]In a preferred embodiment, the invention provides a method wherein the regenerating gene product is only transiently expressed, wherein the regenerating gene product or its coding sequence is not introduced into the genome and gets lost after a certain period after introduction, hereby providing an essentially marker-free transgenic plant as end-product, containing only the desired transgenic nucleic acid, and not the nucleic acid encoding the selection marker used: the regenerating gene product.

[0044]Furthermore, the invention provides plant or plant material obtainable by a method according to the invention, propagated along the lines or using a method herein disclosed. In particular, the invention provides a plant or plant material obtainable by in vitro vegetative or seedless propagation according to the invention from plant starting material, for example using single-node cuttings, axillary branching, regeneration of adventitious organs (roots or shoots), or starting material such as explants or callus tissue or suspensions of, or even single, cells or protoplasts, in particular wherein said starting material comprises transgenic material, said transgenic plant or plant material according to the invention preferably being free of a selection marker gene.

[0045]The invention furthermore provides an isolated and/or recombinant nucleic acid encoding a receptor-like kinase or a functional fragment or functional equivalent thereof, corresponding to or capable of hybridising to a nucleic acid molecule as shown in anyone of FIG. 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20, or its complementary nucleic acid. Such a nucleic is obtained as described above. In a preferred embodiment, such a nucleic acid is at least 75% homologous, preferably at least 85%, more preferably at least 90%, or most preferably at least 95% homologous to a nucleic acid molecule or to a functional equivalent or functional fragment thereof, as shown in anyone of FIG. 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20, or its complementary nucleic acid, for example derived from Arabidopsis thaliana.

[0046]Also, the invention provides a vector comprising a nucleic acid according to the invention. Such a vector is preferably capably of providing stably or transient transformation of a cell by providing said cell with nucleic acid (DNA or RNA) or protein derived from a nucleic acid according to the invention. A variety of methods to provide cells with nucleic acid or protein are known, such as electroporation, liposome-mediated transfer, micro-injection, particle gun bombardment or bacteria-mediated transfer. RNA can for example be produced in vitro from appropriate vector constructs incorporating sites such as SP6, T7 or T3. Protein is produced in vitro in for example yeast or bacterial or insect cells, or other appropriate cells known in the art. DNA can be delivered as linear or circular DNA, possibly placed in a suitable vector for propagation.

1. Furthermore, the invention provides a host cell comprising a nucleic acid or a vector according to the invention. In a preferred embodiment, such a host cell is a transformed cell additionally comprising a desired, but most times totally unrelated, nucleic acid sequence, preferably integrated in a stable fashion in its genome. Even more preferred is a host cell according to the invention wherein the nucleic acid or vector according to the invention is only transiently expressed. Of course it is preferred to use a nucleic acid, vector or host cell according to the invention for use in a culture method as provided by the invention. The invention also provides a method for determining a developmental stage of a plant comprising detecting in said plant or parts thereof a nucleic acid or a proteinaceous substance according to the invention. Said detection is thus aimed at using receptor kinase genes or gene products belonging to the RKS family, or fragments thereof, as markers for plant development.

[0047]The invention furthermore provides an isolated or recombinant proteinaceous substance comprising an amino acid sequence as shown in anyone of FIG. 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20, or a functional equivalent or functional fragment thereof. Proteinaceous substance herein is defined as a substance comprising a peptide, polypeptide or protein, optionally having been modified by for example glycosylation, myristilation, phosphorylation, the addition of lipids, by homologous or heterologous di- or multimerisation, or any other (posttranslational) modifications known in the art.

[0048]Based on sequence composition, the N-terminal domain of predicted amino acid sequences of the RKS gene family represents a signal peptide, indicating that this region of the protein is extracellular. The length of this signal sequence and the predicted cleavage sites have been established using a prediction program: http://genome.cbs.dtu.dk/services/SignalP/. This domain is followed by a short domain containing a number of leucine residues, seperated from each other by 7 amino acid residues. Based on the conservation of these leucines in an amphipathic helix, this domain represents a leucine zipper domain that mediates protein dimerization through formation of a short coiled-coil structure (Landschultz W H, Johnson P F, and McKnight sSL (1988) Science 240, 1759-1764). In RKS proteins, this leucine zipper domain is likely to be involved in receptor hetero/homo dimerization. The next domain contains 2 conserved cysteine residues that forms a disulphate bridge. The subsequent domain represents a leucine rich repeat (LRR) region with 3-5 LRRs of approximately 24 amino acids each. In animals, this domain is known to be involved in protein-protein interactions (Kobe B and Deisenhofer J (1994) TIBS 19, 415-420). In plants the extracellular LRR region is predicted to be necessary for ligand and elicitor binding. At the C-terminal part of the LRR region of most RKS proteins, another conserved couple of cysteine residues is involved in the formation of another disulphate bridge. At both ends, the LRR domain is thus surrounded by two disulphate bridges. The next domain contains a relatively high number of P and S amino acid residues, and shows similarity with cell wall proteins like extensins. Prediction server programs like http://genome.cbs.dtu.dk/services/NetOGlyc/ indicate the presence of multiple O-glycosylation sites within this domain. This domain might have similar functions as extensins and provide interaction sites with multiple cell wall components, thus forming a stable immobilised interaction with the cell wall in which the complete extracellular region of RKS proteins is embedded. The next domain represents a single transmembrane helical domain, as predicted by the program http://genome.cbs.dtu.dk/services/TMHMM-1.0/. The end of this domain, and the beginning of the intracellular cytoplasmic domain, contains a small number of basic K and R residues. The next domain is relatively acidic. The next large domain shows extensive homology with the family of plant serine, threonine receptor kinases. Autophosphorylation studies on SERK (Schmidt et al. 1997) have shown that this domain shows serine, threonine kinase activity. Within the kinase domain, several RKS proteins like RKS0 and RKS8 contain a putative 14-3-3 binding site represented by the core sequence RxpSxP, in which x represents any amino acid (Yaffe M B, Rittinger K, Volinia S, Caron P R, Aitken A, Leffers H, Gamblin S J, Smerdon S J and Cantley L C (1997) Cell 91, 961-971). (Auto)phosphorylation of the S residue within this sequence as a result of ligand-mediated receptor-kinase activation would thus allow the binding and subsequent activation of 14-3-3 proteins. The next domain has an unknown function although the conservation of WD pair residues suggests a function of a docking site for other proteins. The C-terminal intracellular domain contains again part of a single LRR sequence, and might therefore be involved in protein-protein interactions. Preferably such a proteinaceous substance according to the invention is encoded by a nucleic acid according to the invention or produced by a host cell according to the invention.

[0049]In particular, the invention provides a proteinaceous substance for use in a culture method according to the invention. Introduction of a proteinaceous substance into cells or protoplast can be facilitated along the lines of the transformation protocols as known in the art. A variety of methods are known, such as micro-injection, particle gun bombardment or bacteria-mediated transfer. A cell or protoplast thus having been provided with a proteinaceous substance or functional fragment thereof derived from a gene involved in the regulation of plant development can now regenerate on its own, allowing reducing or omitting exogenous phytohormone addition to the culture that comprises that cell or protoplast. The process of vegetative propagation is hereby very much simplified, large numbers of plants with an identical genetic background can now be obtained staring from starting material with the desired characteristics. Proteins or peptides, encoded for by the RKS genes, are produced by expressing the corresponding cDNA sequences, or parts thereof in vitro or in an in vivo expression system in E. coli yeast, Baculovirus or animal cell cultures. The expressed protein sequences are purified using affinity column purification using recombinant Tag sequences attached to the proteins like (HIS)6 tags. Tags are removed after purification by proteolytic cleavage. The resulting protein sequence encodes a functionally active receptor-kinase, or a derivative thereof. In a preferred embodiment, the protein contains a (constitutive) active kinase domain. The purified recombinant protein is introduced into plant cells in order to induce regeneration from these cells in a transient fashion. Proteins are introduced by methods similar as described for the introduction of nucleotide sequences, such as liposome-mediated transfer, micro-injection, electroporation, particle gun bombardment or bacteria-mediated transfer. If so desired, modification of recombinant proteins like glycosylation, disulphate bridge formation, phosphorylation etc. can be optimized in order to obtain an optimal efficiency in protein stability and activity.

[0050]Also, the invention provides an isolated or synthetic antibody specifically recognising a proteinaceous substance according to the invention. Such an antibody is for example obtainable by immunising an experimental animal with a proteinaceous substance according to the invention or an immunogenic fragment or equivalent thereof and harvesting polyclonal antibodies from said immunised animal, or obtainable by other methods known in the art such as by producing monoclonal antibodies, or (single chain) antibodies or binding proteins expressed from recombinant nucleic acid derived from a nucleic acid library, for example obtainable via phage display techniques. Such an antibody can advantageously be used in a culture method according to the invention, for example to identify cells comprising a regenerating gene product as identified above. With such an antibody, the invention also provides a proteinaceous substance specifically recognisable by such an antibody according to the invention, for example obtainable via immunoprecipitation, Western Blotting, or other immunological techniques known in the art. Also, the generation of such antibodies recognising conserved or distinct and specific regions within different members of RKS gene family within a plant species allow the desired isolation of RKS-homologues or recognise a specific RKS gene product in a variety of plant species. These antibodies are also used to screen cDNA expression libraries of plant species to screen for RKS-homologues. The invention, and use as provided of a nucleic acid, a vector, a host cell, a proteinaceous substance or an antibody according to the invention in a method according to the invention is further explained in the detailed description without limiting the invention.

DETAILED DESCRIPTION

[0051]In order to isolate genes involved in the developmental regulation of regeneration in plants, the different members of a family of genes were identified of which the expression was present in developing influorescenses. Within this tissue a large number of different organ primordia are initiated from the influorescence meristems. As a model plant species Arabidopsis thaliana was chosen, based on the presence of many well characterized genetic mutations and the availability of genetic information in databases.

[0052]The differentiation stage is highly stable in vivo, yet in response to nuclear transplantation or cell fusion, the nuclei of differentiated cells exhibit a remarkable capacity to change, both in animal and in plant cells (Blau, 1989). The ability to change the differentiation stage provides cells and tissues with the ability to adapt towards their environment. Normally only a small number of stem cells have the ability to differentiate into different cell types. In plants, the only cells that are truly totipotent are the zygotes, consisting of fused egg cells and sperm. From these dipoid totipotent cells all other differentiated cell types are derived.

[0053]Regeneration is a vegetative reproduction or repair strategy observed in a large number of animal and plant species. Regeneration in plants is defined as the formation of new tissues containing both root and shoot meristems, separate shoot or root meristems, plant organs or organ primordia from individual cells or groups of cells. Regeneration mimics the process of normal cellular and organ differentiation that takes place during plant development and results in the formation of the different plant organs. However, plant cells or groups of cells that under normal conditions are unable to initiate the formation of certain plant organs, meristems or organ primordia can be stimulated by either extracellular stimuli or intracellular modification of the differentiation stage of the cell. Regeneration can take place under either in vivo or in vitro conditions. Regeneration does not include the process of apomixis, wherein specific forms of vegetative plant reproduction are taking place in seeds. Extracellular diffusible factors have shown to be essential for cellular redifferentiation in plant cells (Siegel and Verbeke, 1989). The perception of these signals at the cellular surface and the intracellular signal transduction that finally result in changes in transcriptional regulation provides cells with the ability to respond to such extracellular stimuli.

[0054]In a search for gene products with the ability to regulate cellular differentiation we concentrated on genes involved in perception and transmission of intercellular differentiation signalling. Extracellular signals in animal cells are normally perceived by an high affinity binding compound, the sensor molecule. Extracellular signalling factors are further referred to as ligands and their cellular binding partners are defined as receptors. Upon binding, the extracellular signal can result in modification of the receptor, resulting in transmission of the signal over the cellular membrane. Cell surface receptors contain an extracellular ligand binding domain, a transmembrane domain and an intracellular domain involved in transmission of signals to the intracellular signal transduction components (Walker, 1994). SERK represents a member of the large group of transmembrane receptor kinases with various functions in plants and animals. Many of these gene products are known to be involved in cellular differentiation processes like Clavata 1 (Clark et al. 1997) or Erecta (Torii et al. 1996). Overexpression or mutation of these genes in plants result in morphological changes in plant organs or plant cells.

[0055]The Somatic Embryogenesis Receptor-like Kinase SERK was originally identified as a marker for embryogenic cells, both in vivo, and in vitro. (Schmidt et al. 1997a). Expression of the SERK gene was correlated with the ability to form somatic embryos, a process in which plants are formed from somatic cells through the same morphological, cytological and molecular sequence of stages of embryogenesis as zygotic embryos.

[0056]Transmembrane proteins like receptor kinases provide a set of candidate key regulator gene products that are involved in organ or cellular differentiation. In a search for gene products with the ability to modulate the differentiated we searched for receptor-kinase genes expressed in a plant tissues with a large variety of cellular differentiation processes, the influorescense meristem. In a screen for gene products involved in the regulation of the differentiation stage of cells we identified a complete family of receptor-like kinases.

[0057]Identification of a new family of receptor-like kinases in Arabidopsis thaliana, the RKS gene family.

[0058]In genomic databases of Arabidopsis (accession http://genome-www2.stanford.edu/cgi-bin/AtDB/nph-blast2atdb), a small number of sequences was identified with homology to the Arabidopsis SERK sequence (Schmidt et al. 1997b). These sequences showed homology on nucleotide and predicted amino acid level and were further defined as Receptor Kinases-like SERK (RKS) genes. The initially identified sequences are further defined as RKS₁-5. Based on these five RKS sequences a set of degenerated DNA primers was designed that allowed amplification of possible RKS gene fragments from Arabidopsis.

TABLE-US-00001 Primer RKS B forward: 5'-CC[C/G] AAG AT[C/T] AT[A/T] CAC CG[A/C/T] GAT GT[A/C/G] AA[A/G] GC-3' Primer RKS E reverse 5'-CC[A/G] [A/T]A[A/C/G/T] CC[A/G] AA[A/G] ACA TCG GTT TTC TC-3'

[0059]These sequences are based on conserved parts within the nucleotides encoding one exon of the kinase domain. PCR amplification reactions (60 sec. 94° C.; 60 sec. 50° C.; 90 sec. 72° C.)×40 cycli. were performed with 100 ng of genomic DNA as a template. The resulting PCR products consisted of 209 bp DNA fragments. After cloning in a pGEM-T (Promega) vector, a total of 21 different clones was analysed in order to identify the amplified nucleotide sequences. Removal of the degenerated primer sequences resulted in sequences of 154 nucleotides. Apart from the sequences of RKS1-4 and the SERK gene, a total of 4 new unidentified RKS homologous sequences were identified, further defined as RKS6-10. Sequences from the RKS5 gene were not identified in this screen.

[0060]Number of clones isolated and sequenced for different RKS genes followed by time(s) identified in genomic PCR.

TABLE-US-00002 RKS1 1 RKS2 4 RKS3 2 RKS4 5 RKS5 0 RKS6 2 RKS7 1 RKS8 2 RKS103 SERK/RKS0 1

[0061]These results indicated the presence of at least 9 different sequences with homology to the conserved kinase domain of the predicted RKS genes (apart from SERK) on the Arabidopsis genome (FIG. 1). In order to confirm these data, the fragment of one of the isolated RKS genes was used as a probe in a Southern blot (FIG. 2). Low stringency hybridization confirmed the presence of a number of sequences related to the probe fragment. Under the stringency used (see Materials and Methods) a total of approximately 5 hybridizing bands could be observed, indicating the presence of a small RKS gene family in Arabidopsis.

[0062]RKS gene expression in Arabidopsis inflorescence tissues.

[0063]In order to test whether RKS genes are expressed in tissues where formation of primordia and organs is initiated, RT-PCR reactions were performed on inflorescences. The same combination of PCR primers for RKS fragment amplification was used as described for the genomic PCR reactions. Due to the absence of intron sequences in the described nucleotide fragments, the resulting product was again 209 bp. Starting from the first strand cDNA, a standard PCR reaction was performed for (60 sec. 94° C.; 60 sec. 50° C.; 90 sec. 72° C.)×40 cycli. In order to obtain a sufficient large amounts of amplified product, a reamplification was performed under similar conditions, using 10% of the mix from the first RT-PCR amplification reaction mix as a template. After cloning in a pGEM-T vector, a total of 21 different clones was sequenced in order to identify the amplified sequences. Removal of the degenerated primer sequences resulted in sequences of 154 nucleotides (FIG. 1).

[0064]Number of RT-PCR clones isolated and sequenced for different RKS genes followed by time(s) RT-PCR product identified from influorescence tissue:

TABLE-US-00003 RKS1 0 RKS2 0 RKS3 2 RKS4 5 RKS5 0 RKS6 0 RKS7 1 RKS8 2 RKS104 RKS112 RKS123 RKS131 RKS141 SERK/RKS0 0 RKS 14

[0065]These results indicated the presence of at least 14 different sequences with homology to the conserved kinase domain of the predicted RKS genes (apart from SERK) on the Arabidopsis genome (FIG. 1). Within influorescenses, at least 9 RKS-like genes were expressed. Within this experiment, expression of is RKS 0, 1, 2, 5 and 6 in inflorescences could not be confirmed. Homology between the different RKS sequences was performed using ALLIGMENT software from Geneworks 2.2 (FIG. 3). At least three different subgroups could be visualized of the RKS gene family, representing RKS 2 and RKS6 in subgroup 1, RKS 4, 11, 1, 5, 14 and 7 in subgroup 2 and RKS 0, 8, 10, 12 and 13 in subgroup 3. These results confirmed the hybridization patterns, observed with genomic Southerns hybridized with a member of the RKS subgroup 3 (FIG. 2). A total of 5 hybridizing bands could be observed, that were likely to represent the genes from RKS 0, 8, 10, 12 and 13.

[0066]In order to investigate whether the isolated PCR fragments represented parts of complete RKS genes, full length and partial cDNA clones homologous to these PCR fragments were isolated and characterized.

Isolation and Characterization of the RKS Gene Products in Arabidopsis

[0067]A cDNA library from Arabidopsis thaliana Colombia wild type was used to isolate cDNA clones hybridizing with the PCR amplified RKS gene fragments. The consisted of a BRL λZipLox vector containing SalI, NotI linked cDNA inserts from different plant organs (including siliques, flowers, stems, rosette leaves and roots.

[0068]Filter hybridization, purification of plaques hybridizing under stringent conditions (65° C., 0.1SSC) with the different RKS fragment probes and finally nucleotide sequence analysis resulted in the characterization of a number of RKS cDNA clones. The predicted amino acid sequences of these clones confirmed that the gene products represent members of the RKS plant receptor kinase family RKS. The sequences from the clones identified by the cDNA library were compared and combined with sequence information from the database http://arabidopsis.org/blast/. Apart from 14 different full length cDNA clones a number of 4 different partial clones were identified.

Overexpression of RKS Gene Products in Transgenic Arabidopsis

[0069]Transformation of plasmid DNA into plant cells was performed using A. tumefaciens C58C1. The binary vector used consisted of pGREEN, pGREEN1K or RKS expression constructs. Bacterial colonies were grown on LB agar plates containing 20 mg/L gentamycin, 50 mg/L kanamycin and 50 mg/L rifampicin. Five colonies were used to inoculate 50 ml of LB medium containing 50 mg/L kanamycin and 50 mg/L rifampicin. After 16 hours of incubation at 30° C. cells were concentrated by centrifugation and resuspended in 10 ml infiltration medium (consisting of 5% sucrose and 0.05% Silwett L-77 in water. A helper plasmid, necessary for transformation, consisted of the vector pJIC Sa-Rep and was co-transformed together with the pGREEN vector. After electroporation and incubation for 2 hours at 30° C., cells were plated onto LB plates with 50 mg/L rifampicin en 50 mg/L kanamycin. Arabidopsis thaliana wild-type WS cultivar was transformed following the floral dip protocol (Clough and Bent, 1998). In short, the influorescences of young Arabidopsis WS plants grown under long day conditions (16 hours light, 8 hours dark) were dipped for 10 seconds in 10 ml of infiltration solution. Plants were grown further under long day conditions and seeds were harvested after an additional 3-5 weeks. Seeds were surface sterilized in 4% bleach solution for 15 minutes and after extensive washing in sterile water, plated on 1/2MS plates with 60 mg/L kanamycin. After 10 days incubation under long day conditions, transgenic kanamycin resistant seedlings were isolated and planted on soil for further non-sterile growth under standard long day greenhouse conditions. This infiltration protocol routinely resulted in approximately 1% transformed seeds for each of the RKS gene constructs used.

Regeneration of Arabidopsis Plants After RKS Gene Transformation

[0070]Arabidopsis T2 seeds; obtained from plants infiltrated with A. tumefaciens containing empty pGREEN vectors or pGREEN1K vectors including RKS genes under the control of a 35S promoter, were surface sterilized and added to 40 ml 1/2MS medium culture to which 1 mg/L 2,4-D was added. After three days of stratification at 4° C., the cultures were incubated on a shaker under long day conditions in a climate room of 20° C. for 0-18 days to induce cell proliferation. At different time intervals, seedlings were isolated from the culture, washed and transferred onto 1/2MS agarplates without 2,4-D or any other hormones. Incubation in the climate room was continued under long day conditions for 4 more weeks. In the absence of RKS genes in the transformed binary vector, no regeneration of plantlets could be observed (FIG. 5C). However, in the presence of RKS gene expression, regenerating plants could be observed that originated from the proliferating cell mass (FIGS. 5A,B). Different RKS gene constructs showed the ability to regenerate shoot meristems and leaves. The ability to induce regeneration varied between individual integration events and between RKS gene constructs (FIG. 5A versus 5B). At this timepoint of 4 weeks of regeneration, plantlets were transferred directly to non-sterile soil and grown for another 4-6 weeks under long day conditions. Fertile, seed setting plants could be obtained from the regenerated plantlets as shown in FIGS. 5A,B.

[0071]20 μg of vector DNA for biolistic DNA delivery into Arabidopsis tissue was mixed with a ballistic suspension mix: 10 mg of gold (Aldrich Chem, Co. Gold 1.5-3 micron), 30 μl 5M NaCl, 5 μl 2M Tris pH 8, 965 μL water, 100 μl 0.1M spermidine, 100 μL 25% PEG, 100 μl 2.5M CaCl₂. The suspension was incubated at room temp for 10 min, and centrifuged. The resulting pellet was washed twice with ethanol and resuspended into 200 μl icecold 99.8% ethanol. For each microprojectile bombardment, 10 μl of the gold-coated DNA was used. Bombardment conditions for the HELIUM GUN 461 were: helium pressure 6 bar, vacuum to 50 mbar and 9 cm distance of the tissue from the filter. 0.1 mm mesh size screen was used between tissue and filter, 3 cm distance of the screen from the filter. After bombardment, the Arabidopsis plants were cultured for a period of 3 weeks under long day conditions.

Regeneration in Nicotiana tabacum Induced by Expression of Regeneration-Stimulating Gene Products

[0072]20 microgram of plasmid DNA was transferred into cells of tobacco (NTSR1) leaves, using biolistic bombardment with gold particles coated with DNA. Leaf discs were subsequently submerged in liquid MS30 medium (MS medium 30 g sucrose/l, Murashige and Skoog 1962) containing 1 mg/l kinetin and incubated on a rotary shaker (250 rpm) for 14 days. Leaves were then transferred to plates with MS30 plates, 0.8% agar. All incubations have been performed at 20° C. with 16 hours light, 8 hours dark. Control experiments with empty or control vectors never gave rise to shoot formation. Regenerating plantlets appeared as a result of particle bombardment with regenerating DNA constructs as shown in FIG. 6A-C. The transient nature of the introduced construct could be confirmed for 9 out of 10 different regenerants obtained from bombarded tissue (FIG. 6D).

Induction of Cell Proliferation in Arabidopsis thaliana Induced by Expression of Regeneration Inducing Gene Products

[0073]In order to identify the earlier stages of regeneration after particle bombardment the formation of cellular proliferation was studied as a result of the activity of the regenerating gene product. Single regenerating constructs or combinations of such DNA constructs were bombarded onto two weeks old seedlings of Arabidopsis thaliana grown on MS agar plates. Between one and three weeks thereafter the formation of multicellular structures arising from the surface of bombarded rosette leaves could be observed (FIG. 6E-H). Bombardments with empty control vectors never gave rise to the formation of these structures. Interestingly, the proliferating cell mass originating from bombardment with a GT-W-20S construct developed somatic embryos as a clear indication of regeneration by the process of somatic embryogenesis.

[0074]Somatic embryogenesis was hereby not depending on a tissue culture state of the originating tissue but could be directly initiated on adult leaves still attached to the parent plant. Combinations of different regenerating constructs coated on the same gold particle before bombardment allowed also the process of cellular proliferation to be initiated (FIG. 6G). Multiple loci of proliferated tissue could be observed on individual leaves after the different regenerating constructs (FIG. 6H), indicating that the frequency of regeneration was relatively high when using combinations of regenerating constructs in contrast to bombardments with individual regenerants.

Materials and Methods

Southern Blotting

[0075]10 μg of genomic DNA from Arabidopsis thaliana wildtype was digested with different restriction enzymes. Fragment DNA was size separated on a 0.9% agarosegel. DNA purination was performed in 0.6M NaCl with 0.4M NaOH. Capillairy blotting was performed onto Hybond N+ membranes. Membranes are hybridized overnight at 65° C. in C&G hybridization mix (Church and Gilbert, 1985) and subsequently washed at 65° C. with 5SSC, 0.1% SDS. For detection of radioactivity, the Phosphorimager 425 (Molecular Dynamics) was used in combination with phosphoscreen exposure casettes and ImageQuaNT software.

DNA Fragment Purification

[0076]DE81 paper (Whatmann) was used for isolation of DNA fragments from agarose gels. Paper segments were introduced into the agarosegel just behind the desired DNA fragments (which were visualized under long wave UV with ethidium bromide staining). Electrophoresis was performed for 10 minutes at 10 V/cm gel and the DE81 paper to which the DNA was bound was recovered from the gel. Paper fragments were washed extensively in Low Salt Buffer (LSB) and subsequently DNA was removed from the paper in a small volume of High Salt Buffer (HSB).

TABLE-US-00004 LSB (Low Salt Buffer): HSB (High Salt Buffer): 10 mM Tris pH 7.5 10 mM Tris pH 7.5 1 mM EDTA 1 mM EDTA 100 mM LiCl2 1 M LiCl2 20% Ethanol

Radioactive Probes

[0077]Purified DNA fragments were radiolabelled with 32P-dCTP following a random primed labelling:

[0078]50 ng of fragment DNA in 27 μl water is denatured for 5 min. at 100° C. On ice, 21 μl of GAT mix was added: 0.67 M Hepes, 0.17 M Tris, 17 mM MgCl2, 33 mg/ml acetylated BSA, 25 mg/ml random hexamer primers, 33 mM b-mercapto-ethanol, 5 mM dNTP's (G+A+T) without dCTP. 2 μl dCTP and 2 μl Klenow (1 U/μl) was added, mixed and incubation was performed for 60 min. at 25° C.

Genomic PCR

[0079]Genomic DNA was isolated from wild type Arabidopsis thaliana plants using the protocol of Klimyuk et al. (1993). All PCR reactions were performed in a Thermal Cycler from Perkin Elmer.

[0080]PCR amplification reactions were performed under standard conditions using the following mix: 100 ng genomic template DNA in 5 μl water, denatured for 5 min. at 100° C. On ice the following components were added: 2 μl primer B (10 μM) en 2 ml primer E (10 μM), 1 μl dNTP's (10 mM), 5 μl 10× Taq buffer (Boehringer Mannheim), 0.1 ml Taq polymerase, 5 Units/μl (Boehringer Mannheim), 35 μl water. Paraffin oil was added to the surface in a volume of 20 μl and amplification was performed under the following conditions: (60 sec. 94° C., 60 sec. 50° C., 90 sec. 72° C.)×40 cycli. PCR products were routinely purified using the High Pure-PCR product purification kit (Boehringer Mannheim). Purified DNA was cloned in a five-fold molar excess in the pGEM-T Easy vector (Promega) following standard protocols and reaction mixes as supplied within the reaction kit.

RT-PCR

[0081]Inflorescences from Arabidopsis thaliana was used as source material to isolate total RNA following the protocol of Siebert and Chenchik (1993) 2.5 μg of total RNA in 10 μl of water was linearized by 1 min. incubation at 100° C., followed by the addition of the following components on ice: [0082]2 μl (10 pmol) dT race primer 5'-GAC TCG AGT CGA CAT CGA TTT TTT TTT TTT TT-3' [0083]1 μl dNTP's (10 mM) [0084]4 μl 5×RT buffer (Boehringer Mannheim) [0085]0.8 μl reverse transcriptase M-MuLV Expand (Boehringer Mannheim) [0086]2 μl 100 mM DTT

[0087]Incubation was performed for 60 min. at 42° C., diluted with an equal amount of RNAse free water and stored at -20° C. 2 μl of first strand (=125 ng) was used in PCR reactions, using the RKS degenerated primers B and E. 2 μl primer B (10 μM) en 2 μl primer E (10 μM), 1 μl dNTP's (10 mM), 5 μl 10× Taq buffer (Boehringer Mannheim), 0.1 ml Taq polymerase, 5 Units/μl (Boehringer Mannheim), 38 μl water.

[0088]Paraffin oil was added to the surface in a volume of 20 μl and amplification was performed under the following conditions: (60 sec. 94° C., 60 sec. 50° C., 90 sec. 72° C.)×40 cycli. PCR products were routinely purified using the High Pure-PCR product purification kit from Boehringer Mannheim. Purified DNA was cloned in a five-fold molar excess in the pGEM-T Easy vector (Promega) following standard protocols and reaction mixes as supplied with the reaction kit.

E-coli and A. tumefaciens Transformation

[0089]Transformation of plasmid DNA into competent bacteria was performed by electroporation (Dower et al., 1988), using a Genepulser (Biorad). Conditions for electroporation were as follows: 1.5 kV, 25 mF and 200 W in standard cuvettes. Directly after transformation, cells were incubated for 90 min. at 37° C. in SOC medium (Sambrook et al. 1989). The bacterial suspension was plated on selective agar plates and incubated overnight at 37° C. (E. coli) or for two days at 30° C. (A. tumefaciens) in order to visualize transgenic bacterial colonies.

Nucleotide Sequence Analysis

[0090]Plasmid DNA was isolated from E. coli by standard boiling method protocol (Sambrook et al. 1989) followed by a subsequent purification with the PCR product purification kit from Boehringer Mannheim. Plasmids were sequenced using the ABI PRISM Dye Terminator Cycle Sequencing Core Kit van Perkin Elmer, using standard protocols as designed for the 480 DNA Thermal Cycler. After electrophoresis on polyacrylamide gels, the results were analysed using the 373A DNA Sequencer from Applied Biosystems. Data were analysed using the software programs Sequencer 3.0, Geneworks 2.2 and DNA-strider 1.2.

cDNA Library Screening

[0091]Plating of the cλZipLox cDNA library was performed as described by the supplier protocols (GIBCO BRL), and plaque lifting and purification as described by Sambrook et al. (1989). cDNA library screening was performed using 20 duplicate filters, each containing approximately 250.000 individual plaques. Filters were screened with different RKS DNA probes representing 209 bp amplified PCR fragment. Prior to labelling, DNA fragments were isolated from the pGEM-T vector by digestion and purified twice by DE81 purification from agarose gels. Filters were hybridized under stringent conditions (0.1SSC, 65° C.). Plaques that hybridized on both filters were isolated and used for two subsequent rounds of further purification. The resulting cDNA clones were sequenced using the T7 and SP6 primers from the primer binding regions of the multiple cloning sit of the λZipLox vector. Internal oligos were designed to sequence the complete cDNA inserts of the RKS clones. Only one cDNA clone was sequenced completely for each RKS gene product identified. An alternative approach to identify and subsequently isolate cDNA clones from RKS genes was to screen the Arabidopsis genome database for RKS homologous sequences and to amplify cDNA clones by RT-PCR approach as described above using primers specific for these RKS gene products, based on the sequence data obtained from Arabidopsis genomic databases (accession http://genome-www2.stanford.edu/cgi-bin/AtDB/nph-blast2atdb). Purified RT-PCR products were cloned in a five-fold molar excess in the pGEM-T Easy vector (Promega) following standard protocols and reaction mixes as supplied with the reaction kit.

Regenerating Gene Product Expression Constructs

[0092]The CaMV 35S promoter enhanced by duplication of the -343/-90 bp region (Kay et al, 1987) was isolated from the vector pMON999 together with the NOS terminator by NotI digestion. The resulting construct was cloned into the vector pGreen (Bean et al. 1997) and the resulting binary vector is further defined as pGreen1K. RKS cDNA clones (FIG. 2) were isolated from either the pGEM-T easy vector by EcoRI digestion or from the λZipLox vector by EcoRI/BamHI digestion. The resulting cDNA fragments were cloned into respectively EcoRI digested pGreen 1K or EcoRI/BamHI digested pGreen 1K. Nucleotide sequence analysis was performed in order to test the integrity and the orientation of the RKS cDNA in the vector pGreen1K. The resulting constructs in which the different RKS_0-14 had been ligated in the sense configuration with respect to the 35S promoter are further defined as RKS expression constructs. The other regenerating gene products as previously mentioned have been cloned in a similar fashion into the pGreen expression construct under the control of a 35S promoter

Regeneration Induced by Transient Expression of RKS Gene Products

[0093]Rosette leaves and shoot meristems from 3-weeks old Arabdopsis plants grown under long day conditions were surface sterilized in a 1% bleach solution for 20 min, washed extensively with sterile water and placed on 1/2 MS plates solidified with 0.8% agar.

Particle Bombardment

[0094]20 μg of vector DNA for biolistic DNA delivery into plant tissue was mixed with a ballistic suspension mix: 10 mg of gold (Aldrich Chem, Co. Gold 1.5-3 micron), 30 μl 5M NaCl, 5 μl 2M Tris pH 8.0, 965 μl water, 100 μl 0.1M spermidine, 100 g 25% PEG, 100 μl 2.5M CaCl₂. The suspension was incubated at room temp. for 10 min. and centrifuged. The resulting pellet was washed twice with ethanol and resuspended into 200 μl icecold 99.8% ethanol. For each microprojectile bombardment, 10 μl of the gold-coated DNA was used. Bombardment conditions for the HELIUM GUN 461 were: helium pressure 6 bar, vacuum to 50 mbar and 9 cm distance of the tissue from the filter. 0.1 mm mesh size screen was used between tissue and filter, 3 cm distance of the screen from the filter.

REFERENCES

[0095]Bean S J, Gooding P S, Mullineaux P M and Davies D R (1997) Plant Cell Reports 16, 513-519. [0096]Blau H M (1989) Trends in Genetics 5, 268-272. [0097]Church C and Gilbert K (1985) Proc. Natl. Acad. Sci. USA 81, 1991-1995. [0098]Clark S E, Williams R W and Meyerowitz (1997) Cell 89, 575-585. [0099]Dower W J et al. (1988) Nucl. Acid Res. 16, 6127-6145. [0100]Kay et al. (1987) Science 236, 1299-1302. [0101]Klimyuk V I, Carroll B J, Thomas C M and Jones J D G (1993) Plant J. 3, 493-494. [0102]Sambrook, Fritsch E F and Maniatis T. (1989) Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory, New York. [0103]Schmidt E D L, Hecht V, van Holst G J, de Vries S C (1997b) production of apomictic seed. International publication number WO97/43427. [0104]Schmidt E D L, Guzzo F, Toonen M, de Vries S C (1997a) Development 124, 2049-2062. [0105]Siebert P D and Chenchik A (1993) Nucl. Acid Res. 21, 2019-2020. [0106]Siegel B A and Verbeke J A 1989, Science 244, 580-582. [0107]Torii K U, Mitsukawa N, Oosumi T, Matsuura Y, Yokoyama R, Whittier R F and Komeda Y (1996) Plant Cell 8, 735-746. [0108]Walker J C (1994) Plant Molecular Biology 26, 1599-1609. [0109]Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-496

FIGURE LEGENDS

[0110]FIG. 1 depicts the different 154 bp PCR fragments as amplified with the degenerated forward and reverse RKS primers B and E, as shown in Material and Methods. The sequence of the RKS0 fragment is identical with the corresponding region of the Arabidopsis SERK gene. The nucleotide sequences representing the primer sequences have been deleted from the original 209 bp PCR products in this figure.

[0111]FIG. 2.

[0112]Genomic Southern blot of Arabidopsis thaliana genomic DNA digested with different restriction enzymes. 10 μg of genomic digested DNA is loaded in each lane. Low stringency hybridization (65° C., 5SSC) is performed with a 209 bp PCR fragment encoding part of the kinase domain of RKS0.

[0113]FIG. 3.

[0114]Homologies between the 154 bp fragments as amplified from Arabidopsis with the degenerated RKS primers B and E, shown in FIG. 1. At least three different subgroups can be visualized of the RKS gene family, representing RKS 2 and RKS6 in subgroup 1, RKS 4, 11, 1, 5, 14 and 7 in subgroup 2 and RKS 0, 8, 10, 12 and 13 in subgroup 3. Alignments were performed using DNA Strider 1.2 software.

[0115]FIG. 4a

[0116]Arabidopsis thaliana RKS0 cDNA

[0117]The start codon has been indicated by bold capitals.

[0118]FIG. 4B

[0119]Predicted amino acid sequence of the Arabidopsis thaliana RKS-0 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997).

[0120]At the predicted extracellular domain the first domain represents a signal sequence.

[0121]The second domain contains a leucine zipper motif, containing 4 evenly spaced leucine residues, each separated by 7 other amino acids.

[0122]The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0123]The fourth domain contains a leucine rich repeat domain, consisting of 5 complete repeats of each approximately 24 amino acid residues.

[0124]The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and is a site for O-glycosylation.

[0125]The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned.

[0126]The seventh domain has an unknown function.

[0127]The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions.

[0128]The ninth domain has an unknown function.

[0129]The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0130]FIG. 5

[0131]Proliferated cell mass of Arabidopsis plants transformed with different overexpressing constructs of RKS genes (A and B) or with a control pGREEN1K vector without RKS genes. After 18 days of proliferation in the presence of 2,4-D, tissues have been grown for 4 weeks in the absence of hormones. Regenerated plantlets and green shoots are clearly visible in transformed tissues A and B, but absent in the control tissues transformed with the empty pGREEN vector (C).

[0132]FIG. 6A

[0133]Ballistic bombardment of Nicotiana tabacum leaf discs with GT-W-20S at day 0 is followed by a two weeks submerged culture in liquid MS medium 1 mg/L kinetin. Subsequently the discs are cultured on MS agar plates without hormones. Control experiments with empty vector never gave rise to proliferation. The formation of regenerating from leaf explants is shown in days after bombardment.

[0134]FIG. 6B

[0135]Ballistic bombardment of Nicotiana tabacum leaf discs with GT-SBP5-16S at day 0 is followed by a two weeks submerged culture in liquid MS medium with 1 mg/L kinetin. Subsequently the leaf discs are cultured on MS agar plates without hormones. The formation of regenerating tissues from leaf explants is shown in days after bombardment. Control experiments with empty vectors never gave rise to shoot formation.

[0136]FIG. 6C

[0137]Nicotiana tabacum callus is bombarded with GT-SBP5-16S at day 0. Callus was generated by incubating tobacco leaves for 6 weeks on MS30, 0.8% agar supplemented with 1 mg/L 2,4-D auxin. The callus that formed on the leaves with root like characteristics (extending roots or root hairs from calli) was further cultured on MS30, 0.8% agar petri dishes. The incubation are performed at 20° C. with 16 hours light, 8 hours dark. Control experiments with empty vectors never gave rise to shoot formation. 40 days after bombardment regenerating plant can be identified on top of the bombarded callus tissue (plant 1 and plant 2).

[0138]FIG. 6D

[0139]In order to examine the presence of the bombarded DNA regeneration constructs in regenerated plant, tissue samples were taken from 10 different regenerates from the experiments described in the legends of FIG. 6A-C. Genomic DNA was isolated from all samples, as well as from two control plants. On this DNA a PRC reaction was performed using primers specific for the NptII gene: construct 1 and 3 from experiment I.

Oligo's Used for NptII Specific Amplification:

[0140]Forward oligo: 5'-GCCATGGTGAACAAGATGGATGG-3' Reverse oligo: 5'-GGATCCTCAGAAGAACTCGTCAAG-3'. The resulting PCR product was analysed on agarose gel. Lane 1 and 2 represent regenerates from FIG. 6C; Lane 3-6 represent regenerates from FIG. 6A; Lane 7-10 represent regenerates from FIG. 6B. These 10 plants from which tissue material was isolated for lane 1-10 are shown below just prior to DNA isolation. Lane 11 represents a positive control plant that is stable transformed with a control vector (pG1K-GEP). Lane 12 represents a negative control, an untransformed wildtype NTSR1 plant. Lane 13 and 14 represent positive control E. coli purified DNA used for PCR analysis and M represent marker DNA. Results indicate that only the regenerated plant from lane 8 contained a stable integrated NptII sequence, with all controls giving vector DNA bands.

[0141]FIG. 6E

[0142]Arabidopsis thaliana WS seedlings grown for 14 days on MS agar plates have bombarded with DNA coated gold particles at day 0. Plants are further incubated on the plates at 20° C. with 16 hours light, 8 hours dark. Gold particles were coated with 18 microgram of the construct GT-RKS13. In the bombardment procedure, a GUS expression vector was co-bombarded in combination with the GT-W-20S construct in a molar ration of 10% (GUS versus GT-RKS13). Prior to photography, GUS staining was performed on the bombarded tissues. Cell proliferation (arrow) is detectable on the surface of rosette leaves. Control experiments performed with empty vectors did never result in proliferating tissues.

[0143]FIG. 6F

[0144]Ballistic bombardment of Arabidopsis thaliana with GT-W-20S constructs results in cell proliferation on top of the rosette leaver (left).

[0145]Structures with the morphologic characteristics of somatic embryos appear on the callused structures (middle and right, white arrows). In the bombardment procedure, a GUS expression vector was co-bombarded in combination with the GT-W-20S construct in a molar ration of 10% (GUS versus GT-W-20S). The GT-W-20S construct induces cellular proliferation in neighbouring cells and is unable to induce not contain fragments of the introduced regeneration construct or the GUS expression construct. However, after GUS staining, one cell at the basis of the proliferating cell mass is clearly GUS positive (middle and right, black arrow), indicating that this basal cell has been transformed construct results in the formation of a GUS-negative proliferating cell mass on top of a basal GUS-positive cell. Bombardment studies with empty control vectors did never result in cellular proliferation.

[0146]FIG. 6G

[0147]Ballistic bombardment of Arabidopsis thaliana Ws with GT-CUC2-S, GT-KNAT1-S and GT-CYCD3-S. Cell proliferation becomes already clearly detectable within one week after bombardment (arrow). Control bombardment studies with empty vectors did not result in cellular proliferation.

[0148]FIG. 6H

[0149]Ballistic bombardment of Arabidopsis thaliana Ws with GT-CUC-2S, GT-KNAT2-S and GT-CYCD3-3S. Different regions of cell proliferation within individual rosette leaves become already clearly detectable within one week after bombardment (arrows). Control bombardment studies with empty vectors did not result in cellular proliferation.

[0150]FIG. 7

[0151]The three different RKS subfamilies I-III based on FIG. 3. The predicted protein products are shown, and alignment is based on predicted domain structures. Conserved cysteine residues in disulphate bridge formation are underlined.

[0152]From the N-terminus towards the C-terminus these domains can be defined as the signal sequence, the extracellular region consisting of respectively a leucine zipper domain, a disulphate bridge domain, an leucine rich repeat domain with 3-5 leucine rich repeats, a putative hydroxyproline domain involved in O-glycosylation, a single transmembrane domain, an intracellular region consisting of respectively an anchor domain, a serine/threonine kinase domain, a domain with unknown function and at the C-terminus a sequence resembling an intracellular leucine rich repeat.

[0153]FIG. 8A

[0154]Arabidopsis thaliana RKS1 cDNA

[0155]The start codon has been indicated by bold capitals.

[0156]FIG. 8B

[0157]Predicted amino acid sequence of the Arabidopsis thaliana RKS-1 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0158]The second domain contains a leucine zipper motif, containing 3 leucine residues, each separated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0159]The fourth domain contains a leucine rich repeat domain, consisting of 3 complete repeats of each approximately 24 amino acid residues.

[0160]The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation.

[0161]The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned.

[0162]The seventh domain has an unknown function.

[0163]The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions.

[0164]The ninth domain has an unknown function.

[0165]The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0166]FIG. 9A

[0167]Arabidopsis thaliana RKS2 cDNA. The start codon has been indicated by bold capitals.

[0168]FIG. 9B

[0169]Predicted amino acid sequence of the Arabidopsis thaliana RKS-14 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0170]The second domain contains a leucine zipper motif, containing 2 leucine residues, each separated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0171]The fourth domain contains a leucine rich repeat domain, consisting of 4 complete repeats of each approximately 24 amino acid residues. The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation. The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned. The seventh domain has an unknown function. The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions. The ninth domain has an unknown function. The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0172]FIG. 10a

[0173]Arabidopsis thaliana RKS3 cDNA. The start codon has been indicated by bold capitals.

[0174]FIG. 10B

[0175]Predicted amino acid sequence of the Arabidopsis thaliana RKS-3 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0176]The second domain contains a leucine zipper motif, containing 3 leucine evenly residues, each separated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation. The fourth domain contains a leucine rich repeat domain, consisting of 4 complete repeats of each approximately 24 amino acid residues. The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation. The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned. The seventh domain has an unknown function. The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions. The ninth domain has an unknown function. The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0177]FIG. 11A

[0178]Arabidopsis thaliana RKS4 cDNA

[0179]The start codon has been indicated by bold capitals.

[0180]FIG. 11B

[0181]Predicted amino acid sequence of the Arabidopsis thaliana RKS-4 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0182]The second domain contains a leucine zipper motif, containing 2 leucine residues, each separated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0183]The fourth domain contains a leucine rich repeat domain, consisting of 5 complete repeats of each approximately 24 amino acid residues. The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation. The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned. The seventh domain has an unknown function. The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions. The ninth domain has an unknown function. The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0184]FIG. 12A

[0185]Arabidopsis thaliana RKS5 cDNA. The start codon has been indicated by bold capitals.

[0186]FIG. 12B

[0187]Predicted amino acid sequence of the Arabidopsis thaliana RKS-5 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0188]The second domain contains a leucine zipper motif, containing 2 leucine residues, each separated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0189]The fourth domain contains a leucine rich repeat domain, consisting of 4 complete repeats of each approximately 24 amino acid residues. The fifth domain has no clear function. The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned. The seventh domain has an unknown function. The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions. The ninth domain has an unknown function. The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0190]FIG. 13A

[0191]Arabidopsis thaliana RKS6 cDNA. The start codon has been indicated by bold capitals.

[0192]FIG. 13B

[0193]Predicted amino acid sequence of the Arabidopsis thaliana RKS-6 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0194]The second domain contains a leucine zipper motif, containing 3 leucine residues, each separated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0195]The fourth domain contains a leucine rich repeat domain, consisting of 5 complete repeats of each approximately 24 amino acid residues. The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation. The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned.

[0196]The seventh domain has an unknown function. The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions.

[0197]The ninth domain has an unknown function.

[0198]The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0199]FIG. 14a

[0200]Arabidopsis thaliana RKS8 cDNA.

[0201]The start codon has been indicated by bold capitals.

[0202]FIG. 14B

[0203]Predicted amino acid sequence of the Arabidopsis thaliana RKS-8 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0204]The second domain contains a leucine zipper motif, containing 4 leucine evenly spaced residues, each seperated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0205]The fourth domain contains a leucine rich repeat domain, consisting of 5 complete repeats of each approximately 24 amino acid residues. The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation.

[0206]The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned. The seventh domain has an unknown function.

[0207]The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions.

[0208]The ninth domain has an unknown function. The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0209]FIG. 15A

[0210]Arabidopsis thaliana RKS10 cDNA. The start codon has been indicated by bold capitals.

[0211]FIG. 15B

[0212]Predicted amino acid sequence of the Arabidopsis thaliana RKS-10 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0213]The second domain contains a leucine zipper motif, containing 4 leucine residues, each separated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0214]The fourth domain contains a leucine rich repeat domain, consisting of 4 complete repeats of each approximately 24 amino acid residues. The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation. The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned.

[0215]The seventh domain has an unknown function.

[0216]The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions.

[0217]The ninth domain has an unknown function.

[0218]The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0219]FIG. 16A

[0220]Arabidopsis thaliana RKS11 cDNA/. The start codon has been indicated by bold capitals.

[0221]FIG. 16B

[0222]Predicted amino acid sequence of the Arabidopsis thaliana RKS-11 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence. The second domain contains a leucine zipper motif, containing 3 leucine residues, each separated by 7 other amino acids.

[0223]The third domain contains conserved cysteine residues, involved in disulphate bridge formation. The fourth domain contains a leucine rich repeat domain, consisting of 3 complete repeats of each approximately 24 amino acid residues.

[0224]The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation.

[0225]The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned. The seventh domain has an unknown function.

[0226]The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions.

[0227]The ninth domain has an unknown function. The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0228]FIG. 17a

[0229]Arabidopsis thaliana RKS12 cDNA. The start codon has been indicated by bold capitals.

[0230]FIG. 17B

[0231]Predicted amino acid sequence of the Arabidopsis thaliana RKS-12 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0232]The second domain contains a leucine zipper motif, containing 2 leucine residues, each separated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0233]The fourth domain contains a leucine rich repeat domain, consisting of 4 complete repeats of each approximately 24 amino acid residues. The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation. The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned.

[0234]The seventh domain has an unknown function.

[0235]The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions.

[0236]The ninth domain has an unknown function.

[0237]The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0238]FIG. 18A

[0239]Arabidopsis thaliana RKS13 cDNA. The start codon has been indicated by bold capitals.

[0240]FIG. 18B

[0241]Predicted amino acid sequence of the Arabidopsis thaliana RKS-13 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence.

[0242]The second domain contains a leucine zipper motif, containing 4 leucine residues, each separated by 7 other amino acids. The third domain contains conserved cysteine residues, involved in disulphate bridge formation.

[0243]The fourth domain contains a leucine rich repeat domain, consisting of 4 complete repeats of each approximately 24 amino acid residues. The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation. The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned. The seventh domain has an unknown function. The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions. The ninth domain has an unknown function. The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0244]FIG. 19a

[0245]Arabidopsis thaliana RKS14 cDNA. The start codon has been indicated by bold capitals.

[0246]FIG. 19B

[0247]Predicted amino acid sequence of the Arabidopsis thaliana RKS-14 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). At the predicted extracellular domain the first domain represents a signal sequence. The second domain contains a leucine zipper motif, containing 2 leucine residues, each separated by 7 other amino acids.

[0248]The third domain contains conserved cysteine residues, involved in disulphate bridge formation. The fourth domain contains a leucine rich repeat domain, consisting of 4 complete repeats of each approximately 24 amino acid residues.

[0249]The fifth domain contains many serine and proline residues, and is likely to contain hydroxy-proline residues, and to be a site for O-glycosylation.

[0250]The sixth domain contains a single transmembrane domain after which the predicted intracellular domains are positioned. The seventh domain has an unknown function.

[0251]The eight domain represents a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions.

[0252]The ninth domain has an unknown function. The last and tenth domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0253]FIG. 20 A

[0254]Arabidopsis thaliana RKS 7 partial cDNA sequence.

[0255]The 5'-end and a region between the two cDNA fragments ( . . . ) is not shown.

[0256]FIG. 20B

[0257]Predicted partial amino acid sequences of the Arabidopsis thaliana RKS-7 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as described in Schmidt et al. (1997). The protein sequence is obtained from partial cDNA sequences. The first available domain represents part of a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions. The next domain has an unknown function. The last domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0258]FIG. 21A

[0259]Arabidopsis thaliana RKS 9 partial cDNA sequence.

[0260]The 5'-end is not shown.

[0261]FIG. 21B

[0262]Predicted amino acid sequence of the Arabidopsis thaliana RKS-9 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as descibed in Schmidt et al. (1997). The protein sequence is obtained from partial cDNA sequences. The first available domain represents part of a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions. The next domain has an unknown function. The last domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0263]FIG. 22A

[0264]Arabidopsis thaliana RKS15 partial cDNA sequence.

[0265]The 5'-end is not shown.

[0266]FIG. 22B

[0267]Predicted amino acid sequence of the Arabidopsis thaliana RKS-15 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as descibed in Schmidt et al. (1997). The protein sequence is obtained from partial cDNA sequences. The first available domain represents part of a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions. The next domain has an unknown function. The last domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

[0268]FIG. 23A

[0269]Arabidopsis thaliana RKS16 partial cDNA sequence.

[0270]The 5'-end is not shown.

[0271]FIG. 23B

[0272]Predicted amino acid sequence of the Arabidopsis thaliana RKS-16 protein. Different domains are spaced and shown from the N-terminus towards the C-terminus. Overall domain structure is similar as descibed in Schmidt et al. (1997). The protein sequence is obtained from partial cDNA sequences. The first available domain represents part of a serine/threonine protein kinase domain (Schmidt et al. 1997), and is probably also containing sequences for protein, protein interactions. The next domain has an unknown function. The last domain at the C-terminal end represents a single leucine rich repeat, probably involved in protein, protein interactions.

Sequence CWU 1

67129DNAArabidopsis thalianaDescription of Artificial Sequence primer RKS Bforward 1ccsaagatya twcaccghga tgtvaargc 29226DNAArabidopsis thalianaDescription of Artificial Sequence primer RKS Ereverse 2ccrwanccra aracatcggt tttctc 26332DNAArtificial SequenceDescription of Artificial Sequence primer 3gactcgagtc gacatcgatt tttttttttt tt 32423DNAArtificial SequenceDescription of Artificial Sequence forward oligo 4gccatggtga acaagatgga tgg 23524DNAArtificial SequenceDescription of Artificial Sequence reverse oligo 5ggatcctcag aagaactcgt caag 246154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS1" 6tgaggactga cccgtggata agtactcagg tgcaatgtgg ccaacagttc cacggactgc 60agttgtgaca tgagagtctc tatggtctag aagcttagct aacccgaaat caccaacaac 120tgcttcgaag tcctcatcta acagaatgtt agct 1547154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS2" 7tgacgatttc cctgtggata tacattctgg tgcaatatga cccattgttc ctcggacctg 60agtggttaca ttagtccttc taacatctac caacttggct aaaccaaaat caccaaccac 120tgcttcaaag tcttcatcta gtaacacatt tgca 1548154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS3" 8agatgatttt cctgtgcaga gatactctgg cgcaatgtga cccattgtgc ctcggacttg 60agttgtgaca tgagtcagag atgtgtccac aagcttagct aaaccgaaat ctccaagaac 120tggctcaaaa ttgttgtcta aaagtatgtt tgca 1549154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS4" 9agatgactga ccagtggaga gatactcggg tgcaatgtga ccaacagttc ctctaaccgc 60ggttgtgaca tgtgaatcct cgtggttgag tagctttgct agtccaaaat ccccaacaac 120tgcttcaaaa tactcatcta ggagaatgtt tgct 15410154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS5" 10tgaggactgt ccagtggaaa ggtactcggg agcgatgtgt ccaatggttc ctcggactgc 60ggtagtgaca tgtgaatctc tctggtctaa aagctttgct agaccaaaat cgccaactat 120tgcttcaaag ctctcatcaa gtagaatatt tgca 15411154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS6" 11tgatgatttc cctgttgata aatattctgg tgcaatgtga cccattgttc ctcgaacttg 60agtagtcaca ttagtccttc taacatctac tagcttggct aaaccaaaat caccaaccac 120tgcttcaaaa tcttcatcta gtaacacgtt agct 15412154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS7" 12agaggattga ccagttgaga gatactctgg agcaatgtga cccaccgtgc ctctaaccgc 60ggttgtcaca tgagaatctt gatgatccaa gagtttagct aaaccaaaat cgccaaccac 120agcttcacag tagtcatcaa gaagtatatt cgct 15413154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS8" 13tgaagatttt ccagttgaga gatactcagg agcaatgtgt ccaatagttc cacgcacagc 60cgttgtgaca tgtgtatctt tataatccat aagcctagct aacccgaaat cacctaccac 120cgcctcaaat tcctcgtcca acagaatatt agca 15414154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS10" 14tgatgatttt ccagtggaaa ggtactcagg ggctatatga ccaattgtcc cacgcactgc 60ggttgtcaca tgtgtgtctt tgtagtccat gagttttgca agtccaaaat ccccaaccac 120ggcttcaaac tcttcatcca acaaaatatt tgca 15415154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS11" 15agaagactga ccagtggaga gatattcagg tgcaatgtgg ccaaccgtac cacggaccgc 60agttgtgaca tgagaatccg catggttaag gagctttgcg agtccaaagt caccaacaac 120agcttcaaag cactcgtcta agagaatatt agct 15416154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS12" 16agaagatttt cctgtcgaga ggtactcggg agctatatgg ccaatcgtac cgcgtacagc 60agttgtcaca tgggagtcat tgtaattcat taattttgct agcccaaagt ctccaacaac 120agcttcaaac tcttcatcta acagtatatt tgca 15417154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS13" 17tgctaatata ttgttagatg aagagtttga agctgttgtt ggagattttg ggctcgcaaa 60attaatgaat tataatgact cccatgtgac aactgctgta cgcggtacaa ttggccatat 120agcgcccgag tacctctcga caggaaaatc ttct 15418154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS14" 18tgcgaacata cttcttgacg attactttga agctgttgtc ggagatttcg ggttggctaa 60gcttttggat catgaggagt cgcatgtgac aaccgccgtg agaggaacag tgggtcacat 120tgcacctgag tatctctcaa caggacaatc ttct 15419154DNAArabidopsis thaliana/note="Amplified receptor kinase fragment RKS0" 19tgaagatttt ccggttgaga gatattctgg agcgatgtga ccgatggtgc cacggactgc 60tgttgtcacg tgagtgtctt tatagtccat aagctttgcc aacccgaaat ctccaacaac 120cgcttcgaat tcttcgtcta agaggatgtt tgct 154202087DNAArabidopsis thalianaCDS(193)..(2067)/note="Arabidopsis thaliana RKS0 cDNA" 20atttttattt tattttttac tctttgtttg ttttaatgct aatgggtttt taaaagggtt 60atcgaaaaaa tgagtgagtt tgtgttgagg ttgtctctgt aaagtgttaa tggtggtgat 120tttcggaagt tagggttttc tcggatctga agagatcaaa tcaagattcg aaatttacca 180ttgttgtttg aa atg gag tcg agt tat gtg gtg ttt atc tta ctt tca ctg 231Met Glu Ser Ser Tyr Val Val Phe Ile Leu Leu Ser Leu1 5 10atc tta ctt ccg aat cat tca ctg tgg ctt gct tct gct aat ttg gaa 279Ile Leu Leu Pro Asn His Ser Leu Trp Leu Ala Ser Ala Asn Leu Glu15 20 25ggt gat gct ttg cat act ttg agg gtt act cta gtt gat cca aac aat 327Gly Asp Ala Leu His Thr Leu Arg Val Thr Leu Val Asp Pro Asn Asn30 35 40 45gtc ttg cag agc tgg gat cct acg cta gtg aat cct tgc aca tgg ttc 375Val Leu Gln Ser Trp Asp Pro Thr Leu Val Asn Pro Cys Thr Trp Phe50 55 60cat gtc act tgc aac aac gag aac agt gtc ata aga gtt gat ttg ggg 423His Val Thr Cys Asn Asn Glu Asn Ser Val Ile Arg Val Asp Leu Gly65 70 75aat gca gag tta tct ggc cat tta gtt cca gag ctt ggt gtg ctc aag 471Asn Ala Glu Leu Ser Gly His Leu Val Pro Glu Leu Gly Val Leu Lys80 85 90aat ttg cag tat ttg gag ctt tac agt aac aac ata act ggc ccg att 519Asn Leu Gln Tyr Leu Glu Leu Tyr Ser Asn Asn Ile Thr Gly Pro Ile95 100 105cct agt aat ctt gga aat ctg aca aac tta gtg agt ttg gat ctt tac 567Pro Ser Asn Leu Gly Asn Leu Thr Asn Leu Val Ser Leu Asp Leu Tyr110 115 120 125tta aac agc ttc tcc ggt cct att ccg gaa tca ttg gga aag ctt tca 615Leu Asn Ser Phe Ser Gly Pro Ile Pro Glu Ser Leu Gly Lys Leu Ser130 135 140aag ctg aga ttt ctc cgg ctt aac aac aac agt ctc act ggg tca att 663Lys Leu Arg Phe Leu Arg Leu Asn Asn Asn Ser Leu Thr Gly Ser Ile145 150 155cct atg tca ctg acc aat att act acc ctt caa gtg tta gat cta tca 711Pro Met Ser Leu Thr Asn Ile Thr Thr Leu Gln Val Leu Asp Leu Ser160 165 170aat aac aga ctc tct ggt tca gtt cct gac aat ggc tcc ttc tca ctc 759Asn Asn Arg Leu Ser Gly Ser Val Pro Asp Asn Gly Ser Phe Ser Leu175 180 185ttc aca ccc atc agt ttt gct aat aac tta gac cta tgt gga cct gtt 807Phe Thr Pro Ile Ser Phe Ala Asn Asn Leu Asp Leu Cys Gly Pro Val190 195 200 205aca agt cac cca tgt cct gga tct ccc ccg ttt tct cct cca cca cct 855Thr Ser His Pro Cys Pro Gly Ser Pro Pro Phe Ser Pro Pro Pro Pro210 215 220ttt att caa cct ccc cca gtt tcc acc ccg agt ggg tat ggt ata act 903Phe Ile Gln Pro Pro Pro Val Ser Thr Pro Ser Gly Tyr Gly Ile Thr225 230 235gga gca ata gct ggt gga gtt gct gca ggt gct gct ttg ccc ttt gct 951Gly Ala Ile Ala Gly Gly Val Ala Ala Gly Ala Ala Leu Pro Phe Ala240 245 250gct cct gca ata gcc ttt gct tgg tgg cga cga aga agc cca cta gat 999Ala Pro Ala Ile Ala Phe Ala Trp Trp Arg Arg Arg Ser Pro Leu Asp255 260 265att ttc ttc gat gtc cct gcc gaa gaa gat cca gaa gtt cat ctg gga 1047Ile Phe Phe Asp Val Pro Ala Glu Glu Asp Pro Glu Val His Leu Gly270 275 280 285cag ctc aag agg ttt tct ttg cgg gag cta caa gtg gcg agt gat ggg 1095Gln Leu Lys Arg Phe Ser Leu Arg Glu Leu Gln Val Ala Ser Asp Gly290 295 300ttt agt aac aag aac att ttg ggc aga ggt ggg ttt ggg aaa gtc tac 1143Phe Ser Asn Lys Asn Ile Leu Gly Arg Gly Gly Phe Gly Lys Val Tyr305 310 315aag gga cgc ttg gca gac gga act ctt gtt gct gtc aag aga ctg aag 1191Lys Gly Arg Leu Ala Asp Gly Thr Leu Val Ala Val Lys Arg Leu Lys320 325 330gaa gag cga act cca ggt gga gag ctc cag ttt caa aca gaa gta gag 1239Glu Glu Arg Thr Pro Gly Gly Glu Leu Gln Phe Gln Thr Glu Val Glu335 340 345atg ata agt atg gca gtt cat cga aac ctg ttg aga tta cga ggt ttc 1287Met Ile Ser Met Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly Phe350 355 360 365tgt atg aca ccg acc gag aga ttg ctt gtg tat cct tac atg gcc aat 1335Cys Met Thr Pro Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn370 375 380gga agt gtt gct tcg tgt ctc aga gag agg cca ccg tca caa cct ccg 1383Gly Ser Val Ala Ser Cys Leu Arg Glu Arg Pro Pro Ser Gln Pro Pro385 390 395ctt gat tgg cca acg cgg aag aga atc gcg cta ggc tca gct cga ggt 1431Leu Asp Trp Pro Thr Arg Lys Arg Ile Ala Leu Gly Ser Ala Arg Gly400 405 410ttg tct tac cta cat gat cac tgc gat ccg aag atc att cac cgt gac 1479Leu Ser Tyr Leu His Asp His Cys Asp Pro Lys Ile Ile His Arg Asp415 420 425gta aaa gca gca aac atc ctc tta gac gaa gaa ttc gaa gcg gtt gtt 1527Val Lys Ala Ala Asn Ile Leu Leu Asp Glu Glu Phe Glu Ala Val Val430 435 440 445gga gat ttc ggg ttg gca aag ctt atg gac tat aaa gac act cac gtg 1575Gly Asp Phe Gly Leu Ala Lys Leu Met Asp Tyr Lys Asp Thr His Val450 455 460aca aca gca gtc cgt ggc acc atc ggt cac atc gct cca gaa tat ctc 1623Thr Thr Ala Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu465 470 475tca acc gga aaa tct tca gag aaa acc gac gtt ttc gga tac gga atc 1671Ser Thr Gly Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Ile480 485 490atg ctt cta gaa cta atc aca gga caa aga gct ttc gat ctc gct cgg 1719Met Leu Leu Glu Leu Ile Thr Gly Gln Arg Ala Phe Asp Leu Ala Arg495 500 505cta gct aac gac gac gac gtc atg tta ctt gac tgg gtg aaa gga ttg 1767Leu Ala Asn Asp Asp Asp Val Met Leu Leu Asp Trp Val Lys Gly Leu510 515 520 525ttg aag gag aag aag cta gag atg tta gtg gat cca gat ctt caa aca 1815Leu Lys Glu Lys Lys Leu Glu Met Leu Val Asp Pro Asp Leu Gln Thr530 535 540aac tac gag gag aga gaa ctg gaa caa gtg ata caa gtg gcg ttg cta 1863Asn Tyr Glu Glu Arg Glu Leu Glu Gln Val Ile Gln Val Ala Leu Leu545 550 555tgc acg caa gga tca cca atg gaa aga cca aag atg tct gaa gtt gta 1911Cys Thr Gln Gly Ser Pro Met Glu Arg Pro Lys Met Ser Glu Val Val560 565 570agg atg ctg gaa gga gat ggg ctt gcg gag aaa tgg gac gaa tgg caa 1959Arg Met Leu Glu Gly Asp Gly Leu Ala Glu Lys Trp Asp Glu Trp Gln575 580 585aaa gtt gag att ttg agg gaa gag att gat ttg agt cct aat cct aac 2007Lys Val Glu Ile Leu Arg Glu Glu Ile Asp Leu Ser Pro Asn Pro Asn590 595 600 605tct gat tgg att ctt gat tct act tac aat ttg cac gcc gtt gag tta 2055Ser Asp Trp Ile Leu Asp Ser Thr Tyr Asn Leu His Ala Val Glu Leu610 615 620tct ggt cca agg taaaaaaaaa aaaaaaaaaa 2087Ser Gly Pro Arg625 21625PRTArabidopsis thaliana 21Met Glu Ser Ser Tyr Val Val Phe Ile Leu Leu Ser Leu Ile Leu Leu1 5 10 15Pro Asn His Ser Leu Trp Leu Ala Ser Ala Asn Leu Glu Gly Asp Ala20 25 30Leu His Thr Leu Arg Val Thr Leu Val Asp Pro Asn Asn Val Leu Gln35 40 45Ser Trp Asp Pro Thr Leu Val Asn Pro Cys Thr Trp Phe His Val Thr50 55 60Cys Asn Asn Glu Asn Ser Val Ile Arg Val Asp Leu Gly Asn Ala Glu65 70 75 80Leu Ser Gly His Leu Val Pro Glu Leu Gly Val Leu Lys Asn Leu Gln85 90 95Tyr Leu Glu Leu Tyr Ser Asn Asn Ile Thr Gly Pro Ile Pro Ser Asn100 105 110Leu Gly Asn Leu Thr Asn Leu Val Ser Leu Asp Leu Tyr Leu Asn Ser115 120 125Phe Ser Gly Pro Ile Pro Glu Ser Leu Gly Lys Leu Ser Lys Leu Arg130 135 140Phe Leu Arg Leu Asn Asn Asn Ser Leu Thr Gly Ser Ile Pro Met Ser145 150 155 160Leu Thr Asn Ile Thr Thr Leu Gln Val Leu Asp Leu Ser Asn Asn Arg165 170 175Leu Ser Gly Ser Val Pro Asp Asn Gly Ser Phe Ser Leu Phe Thr Pro180 185 190Ile Ser Phe Ala Asn Asn Leu Asp Leu Cys Gly Pro Val Thr Ser His195 200 205Pro Cys Pro Gly Ser Pro Pro Phe Ser Pro Pro Pro Pro Phe Ile Gln210 215 220Pro Pro Pro Val Ser Thr Pro Ser Gly Tyr Gly Ile Thr Gly Ala Ile225 230 235 240Ala Gly Gly Val Ala Ala Gly Ala Ala Leu Pro Phe Ala Ala Pro Ala245 250 255Ile Ala Phe Ala Trp Trp Arg Arg Arg Ser Pro Leu Asp Ile Phe Phe260 265 270Asp Val Pro Ala Glu Glu Asp Pro Glu Val His Leu Gly Gln Leu Lys275 280 285Arg Phe Ser Leu Arg Glu Leu Gln Val Ala Ser Asp Gly Phe Ser Asn290 295 300Lys Asn Ile Leu Gly Arg Gly Gly Phe Gly Lys Val Tyr Lys Gly Arg305 310 315 320Leu Ala Asp Gly Thr Leu Val Ala Val Lys Arg Leu Lys Glu Glu Arg325 330 335Thr Pro Gly Gly Glu Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser340 345 350Met Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr355 360 365Pro Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val370 375 380Ala Ser Cys Leu Arg Glu Arg Pro Pro Ser Gln Pro Pro Leu Asp Trp385 390 395 400Pro Thr Arg Lys Arg Ile Ala Leu Gly Ser Ala Arg Gly Leu Ser Tyr405 410 415Leu His Asp His Cys Asp Pro Lys Ile Ile His Arg Asp Val Lys Ala420 425 430Ala Asn Ile Leu Leu Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe435 440 445Gly Leu Ala Lys Leu Met Asp Tyr Lys Asp Thr His Val Thr Thr Ala450 455 460Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly465 470 475 480Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Ile Met Leu Leu485 490 495Glu Leu Ile Thr Gly Gln Arg Ala Phe Asp Leu Ala Arg Leu Ala Asn500 505 510Asp Asp Asp Val Met Leu Leu Asp Trp Val Lys Gly Leu Leu Lys Glu515 520 525Lys Lys Leu Glu Met Leu Val Asp Pro Asp Leu Gln Thr Asn Tyr Glu530 535 540Glu Arg Glu Leu Glu Gln Val Ile Gln Val Ala Leu Leu Cys Thr Gln545 550 555 560Gly Ser Pro Met Glu Arg Pro Lys Met Ser Glu Val Val Arg Met Leu565 570 575Glu Gly Asp Gly Leu Ala Glu Lys Trp Asp Glu Trp Gln Lys Val Glu580 585 590Ile Leu Arg Glu Glu Ile Asp Leu Ser Pro Asn Pro Asn Ser Asp Trp595 600 605Ile Leu Asp Ser Thr Tyr Asn Leu His Ala Val Glu Leu Ser Gly Pro610 615 620Arg62522625PRTArabidopsis thaliana/note="Predicted amino acid sequence of the Arabidopsis thaliana RKS-0 protein" 22Met Glu Ser Ser Tyr Val Val Phe Ile Leu Leu Ser Leu Ile Leu Leu1 5 10 15Pro Asn His Ser Leu Trp Leu Ala Ser Ala Asn Leu Glu Gly Asp Ala20 25 30Leu His Thr Leu Arg Val Thr Leu Val Asp Pro Asn Asn Val Leu Gln35 40 45Ser Trp Asp Pro Thr Leu Val Asn Pro Cys Thr Trp Phe His Val Thr50 55 60Cys Asn Asn Glu Asn Ser Val Ile Arg Val Asp Leu Gly Asn Ala Glu65 70 75 80Leu Ser Gly His Leu Val Pro Glu Leu Gly Val Leu Lys Asn Leu Gln85 90 95Tyr Leu Glu Leu Tyr Ser Asn Asn Ile Thr Gly Pro Ile Pro Ser Asn100 105 110Leu Gly Asn Leu Thr Asn Leu Val Ser Leu Asp Leu Tyr Leu Asn Ser115 120 125Phe Ser Gly Pro Ile Pro Glu Ser Leu Gly Lys Leu Ser Lys Leu Arg130 135 140Phe Leu Arg Leu Asn Asn Asn Ser Leu Thr Gly Ser Ile Pro Met Ser145 150 155 160Leu Thr Asn Ile Thr Thr Leu Gln Val Leu Asp Leu Ser Asn Asn Arg165 170 175Leu Ser Gly Ser Val Pro Asp Asn Gly Ser Phe Ser Leu Phe Thr Pro180 185 190Ile

Ser Phe Ala Asn Asn Leu Asp Leu Cys Gly Pro Val Thr Ser His195 200 205Pro Cys Pro Gly Ser Pro Pro Phe Ser Pro Pro Pro Pro Phe Ile Gln210 215 220Pro Pro Pro Val Ser Thr Pro Ser Gly Tyr Gly Ile Thr Gly Ala Ile225 230 235 240Ala Gly Gly Val Ala Ala Gly Ala Ala Leu Pro Phe Ala Ala Pro Ala245 250 255Ile Ala Phe Ala Trp Trp Arg Arg Arg Ser Pro Leu Asp Ile Phe Phe260 265 270Asp Val Pro Ala Glu Glu Asp Pro Glu Val His Leu Gly Gln Leu Lys275 280 285Arg Phe Ser Leu Arg Glu Leu Gln Val Ala Ser Asp Gly Phe Ser Asn290 295 300Lys Asn Ile Leu Gly Arg Gly Gly Phe Gly Lys Val Tyr Lys Gly Arg305 310 315 320Leu Ala Asp Gly Thr Leu Val Ala Val Lys Arg Leu Lys Glu Glu Arg325 330 335Thr Pro Gly Gly Glu Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser340 345 350Met Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr355 360 365Pro Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val370 375 380Ala Ser Cys Leu Arg Glu Arg Pro Pro Ser Gln Pro Pro Leu Asp Trp385 390 395 400Pro Thr Arg Lys Arg Ile Ala Leu Gly Ser Ala Arg Gly Leu Ser Tyr405 410 415Leu His Asp His Cys Asp Pro Lys Ile Ile His Arg Asp Val Lys Ala420 425 430Ala Asn Ile Leu Leu Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe435 440 445Gly Leu Ala Lys Leu Met Asp Tyr Lys Asp Thr His Val Thr Thr Ala450 455 460Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly465 470 475 480Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Ile Met Leu Leu485 490 495Glu Leu Ile Thr Gly Gln Arg Ala Phe Asp Leu Ala Arg Leu Ala Asn500 505 510Asp Asp Asp Val Met Leu Leu Asp Trp Val Lys Gly Leu Leu Lys Glu515 520 525Lys Lys Leu Glu Met Leu Val Asp Pro Asp Leu Gln Thr Asn Tyr Glu530 535 540Glu Arg Glu Leu Glu Gln Val Ile Gln Val Ala Leu Leu Cys Thr Gln545 550 555 560Gly Ser Pro Met Glu Arg Pro Lys Met Ser Glu Val Val Arg Met Leu565 570 575Glu Gly Asp Gly Leu Ala Glu Lys Trp Asp Glu Trp Gln Lys Val Glu580 585 590Ile Leu Arg Glu Glu Ile Asp Leu Ser Pro Asn Pro Asn Ser Asp Trp595 600 605Ile Leu Asp Ser Thr Tyr Asn Leu His Ala Val Glu Leu Ser Gly Pro610 615 620Arg62523613PRTArabidopsis thaliana/note="Predicted protein domain RKS-6 of the RKS subfamily I" 23Met Arg Met Phe Ser Leu Gln Lys Met Ala Met Ala Phe Thr Leu Leu1 5 10 15Phe Phe Ala Cys Leu Cys Ser Phe Val Ser Pro Asp Ala Gln Gly Asp20 25 30Ala Leu Phe Ala Leu Arg Ile Ser Leu Arg Ala Leu Pro Asn Gln Leu35 40 45Ser Asp Trp Asn Gln Asn Gln Val Asn Pro Cys Thr Trp Ser Gln Val50 55 60Ile Cys Asp Asp Lys Asn Phe Val Thr Ser Leu Thr Leu Ser Asp Met65 70 75 80Asn Phe Ser Gly Thr Leu Ser Ser Arg Val Gly Ile Leu Glu Asn Leu85 90 95Lys Thr Leu Thr Leu Lys Gly Asn Gly Ile Thr Gly Glu Ile Pro Glu100 105 110Asp Phe Gly Asn Leu Thr Ser Leu Thr Ser Leu Asp Leu Glu Asp Asn115 120 125Gln Leu Thr Gly Arg Ile Pro Ser Thr Ile Gly Asn Leu Lys Lys Leu130 135 140Gln Phe Leu Thr Leu Ser Arg Asn Lys Leu Asn Gly Thr Ile Pro Glu145 150 155 160Ser Leu Thr Gly Leu Pro Asn Leu Leu Asn Leu Leu Leu Asp Ser Asn165 170 175Ser Leu Ser Gly Gln Ile Pro Gln Ser Leu Phe Glu Ile Pro Lys Tyr180 185 190Asn Phe Thr Ser Asn Asn Leu Asn Cys Gly Gly Arg Gln Pro His Pro195 200 205Cys Val Ser Ala Val Ala His Ser Gly Asp Ser Ser Lys Pro Lys Thr210 215 220Gly Ile Ile Ala Gly Val Val Ala Gly Val Thr Val Val Leu Phe Gly225 230 235 240Ile Leu Leu Phe Leu Phe Cys Lys Asp Arg His Lys Gly Tyr Arg Arg245 250 255Asp Val Phe Val Asp Val Ala Gly Glu Val Asp Arg Arg Ile Ala Phe260 265 270Gly Gln Leu Lys Arg Phe Ala Trp Arg Glu Leu Gln Leu Ala Thr Asp275 280 285Asn Phe Ser Glu Lys Asn Val Leu Gly Gln Gly Gly Phe Gly Lys Val290 295 300Tyr Lys Gly Val Leu Pro Asp Thr Pro Lys Val Ala Val Lys Arg Leu305 310 315 320Thr Asp Phe Glu Ser Pro Gly Gly Asp Ala Ala Phe Gln Arg Glu Val325 330 335Glu Met Ile Ser Val Ala Val His Arg Asn Leu Leu Arg Leu Ile Gly340 345 350Phe Cys Thr Thr Gln Thr Glu Arg Leu Leu Val Tyr Pro Phe Met Gln355 360 365Asn Leu Ser Leu Ala His Arg Leu Arg Glu Ile Lys Ala Gly Asp Pro370 375 380Val Leu Asp Trp Glu Thr Arg Lys Arg Ile Ala Leu Gly Ala Ala Arg385 390 395 400Gly Phe Glu Tyr Leu His Glu His Cys Asn Pro Lys Ile Ile His Arg405 410 415Asp Val Lys Ala Ala Asn Val Leu Leu Asp Glu Asp Phe Glu Ala Val420 425 430Val Gly Asp Phe Gly Leu Ala Lys Leu Val Asp Val Arg Arg Thr Asn435 440 445Val Thr Thr Gln Val Arg Gly Thr Met Gly His Ile Ala Pro Glu Tyr450 455 460Leu Ser Thr Gly Lys Ser Ser Glu Arg Thr Asp Val Phe Gly Tyr Gly465 470 475 480Ile Met Leu Leu Glu Leu Val Thr Gly Gln Arg Ala Ile Asp Phe Ser485 490 495Arg Leu Glu Glu Glu Asp Asp Val Leu Leu Leu Asp His Val Lys Lys500 505 510Leu Glu Arg Glu Lys Arg Leu Gly Ala Ile Val Asp Lys Asn Leu Asp515 520 525Gly Glu Tyr Ile Lys Glu Glu Val Glu Met Met Ile Gln Val Ala Leu530 535 540Leu Cys Thr Gln Gly Ser Pro Glu Asp Arg Pro Val Met Ser Glu Val545 550 555 560Val Arg Met Leu Glu Gly Glu Gly Leu Ala Glu Arg Trp Glu Glu Trp565 570 575Gln Asn Val Glu Val Thr Arg Arg His Glu Phe Glu Arg Leu Gln Arg580 585 590Arg Phe Asp Trp Gly Glu Asp Ser Met His Asn Gln Asp Ala Ile Glu595 600 605Leu Ser Gly Gly Arg61024571PRTArabidopsis thaliana/note="Predicted protein domain RKS-2 of the RKS subfamily I" 24Met Ala Leu Leu Ile Ile Thr Ala Leu Val Phe Ser Ser Leu Trp Ser1 5 10 15Ser Val Ser Pro Asp Ala Gln Gly Asp Ala Leu Phe Ala Leu Arg Ser20 25 30Ser Leu Arg Ala Ser Pro Glu Gln Leu Ser Asp Trp Asn Gln Asn Gln35 40 45Val Asp Pro Cys Thr Trp Ser Gln Val Ile Cys Asp Asp Lys Lys His50 55 60Val Thr Ser Val Thr Leu Ser Tyr Met Asn Phe Ser Ser Gly Thr Leu65 70 75 80Ser Ser Gly Ile Gly Ile Leu Thr Thr Leu Lys Thr Leu Thr Leu Lys85 90 95Gly Asn Gly Ile Met Gly Gly Ile Pro Glu Ser Ile Gly Asn Leu Ser100 105 110Ser Leu Thr Ser Leu Asp Leu Glu Asp Asn His Leu Thr Asp Arg Ile115 120 125Pro Ser Thr Leu Gly Asn Leu Lys Asn Leu Gln Phe Phe Phe Thr Ala130 135 140Asn Asn Leu Ser Cys Gly Gly Thr Phe Pro Gln Pro Cys Val Thr Glu145 150 155 160Ser Ser Pro Ser Gly Asp Ser Ser Ser Arg Lys Thr Gly Ile Ile Ala165 170 175Gly Val Val Ser Gly Ile Ala Val Ile Leu Leu Gly Phe Phe Phe Phe180 185 190Phe Phe Cys Lys Asp Lys His Lys Gly Tyr Lys Arg Asp Val Phe Val195 200 205Asp Val Ala Gly Thr Asn Phe Lys Lys Gly Leu Ile Ser Gly Glu Val210 215 220Asp Arg Arg Ile Ala Phe Gly Gln Leu Arg Arg Phe Ala Trp Arg Glu225 230 235 240Leu Gln Leu Ala Thr Asp Glu Phe Ser Glu Lys Asn Val Leu Gly Gln245 250 255Gly Gly Phe Gly Lys Val Tyr Lys Gly Leu Leu Ser Asp Gly Thr Lys260 265 270Val Ala Val Lys Arg Leu Thr Asp Phe Glu Arg Pro Gly Gly Asp Glu275 280 285Ala Phe Gln Arg Glu Val Glu Met Ile Ser Val Ala Val His Arg Asn290 295 300Leu Leu Arg Leu Ile Gly Phe Cys Thr Thr Gln Thr Glu Arg Leu Leu305 310 315 320Val Tyr Pro Phe Met Gln Asn Leu Ser Val Ala Tyr Cys Leu Arg Glu325 330 335Ile Lys Pro Gly Asp Pro Val Leu Asp Trp Phe Arg Arg Lys Gln Ile340 345 350Ala Leu Gly Ala Ala Arg Gly Leu Glu Tyr Leu His Glu His Cys Asn355 360 365Pro Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Val Leu Leu Asp370 375 380Glu Asp Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala Lys Leu Val385 390 395 400Asp Val Arg Arg Thr Asn Val Thr Thr Gln Val Arg Gly Thr Met Gly405 410 415His Ile Ala Pro Glu Cys Ile Ser Thr Gly Lys Ser Ser Glu Lys Thr420 425 430Asp Val Phe Gly Tyr Gly Ile Met Leu Leu Glu Leu Val Thr Gly Gln435 440 445Arg Ala Ile Asp Phe Ser Arg Leu Glu Glu Glu Asp Asp Val Leu Leu450 455 460Leu Asp His Val Lys Lys Leu Glu Arg Glu Lys Arg Leu Glu Asp Ile465 470 475 480Val Asp Lys Lys Leu Asp Glu Asp Tyr Ile Lys Glu Glu Val Glu Met485 490 495Met Ile Gln Val Ala Leu Leu Cys Thr Gln Ala Ala Pro Glu Glu Arg500 505 510Pro Ala Met Ser Glu Val Val Arg Met Leu Glu Gly Glu Gly Leu Ala515 520 525Glu Arg Trp Glu Glu Trp Gln Asn Leu Glu Val Thr Arg Gln Glu Glu530 535 540Phe Gln Arg Leu Gln Arg Arg Phe Asp Trp Gly Glu Asp Ser Ile Asn545 550 555 560Asn Gln Asp Ala Ile Glu Leu Ser Gly Gly Arg565 57025578PRTArabidopsis thaliana/note="Predicted protein domain RKS-3 of the RKS subfamily I" 25Met Ala Leu Ala Phe Val Gly Ile Thr Ser Ser Thr Thr Gln Pro Asp1 5 10 15Ile Glu Gly Gly Ala Leu Leu Gln Leu Arg Asp Ser Leu Asn Asp Ser20 25 30Ser Asn Arg Leu Lys Trp Thr Arg Asp Phe Val Ser Pro Cys Tyr Ser35 40 45Trp Ser Tyr Val Thr Cys Arg Gly Gln Ser Val Val Ala Leu Asn Leu50 55 60Ala Ser Ser Gly Phe Thr Gly Thr Leu Ser Pro Ala Ile Thr Lys Leu65 70 75 80Lys Phe Leu Val Thr Leu Glu Leu Gln Asn Asn Ser Leu Ser Gly Ala85 90 95Leu Pro Asp Ser Leu Gly Asn Met Val Asn Leu Gln Thr Leu Asn Leu100 105 110Ser Val Asn Ser Phe Ser Gly Ser Ile Pro Ala Ser Trp Ser Gln Leu115 120 125Ser Asn Leu Lys His Leu Asp Leu Ser Ser Asn Asn Leu Thr Gly Ser130 135 140Ile Pro Thr Gln Phe Phe Ser Ile Pro Thr Phe Glu Phe Ser Gly Thr145 150 155 160Gln Leu Ile Cys Gly Lys Ser Leu Asn Gln Pro Cys Ser Ser Ser Arg165 170 175Leu Pro Val Thr Ser Ser Lys Lys Lys Leu Arg Asp Ile Thr Leu Thr180 185 190Ala Ser Cys Val Ala Ser Ile Ile Leu Phe Leu Gly Ala Met Val Met195 200 205Tyr His His His Arg Val Arg Arg Thr Lys Tyr Asp Ile Phe Phe Asp210 215 220Val Ala Gly Glu Asp Asp Arg Lys Ile Ser Phe Gly Gln Leu Lys Arg225 230 235 240Phe Ser Leu Arg Glu Ile Gln Leu Ala Thr Asp Ser Phe Asn Glu Ser245 250 255Asn Leu Ile Gly Gln Gly Gly Phe Gly Lys Val Tyr Arg Gly Leu Leu260 265 270Pro Asp Lys Thr Lys Val Ala Val Lys Arg Leu Ala Asp Tyr Phe Ser275 280 285Pro Gly Gly Glu Ala Ala Phe Gln Arg Glu Ile Gln Leu Ile Ser Val290 295 300Ala Val His Lys Asn Leu Leu Arg Leu Ile Gly Phe Cys Thr Thr Ser305 310 315 320Ser Glu Arg Ile Leu Val Tyr Pro Tyr Met Glu Asn Leu Ser Val Ala325 330 335Tyr Arg Leu Arg Asp Leu Lys Ala Gly Glu Glu Gly Leu Asp Trp Pro340 345 350Thr Arg Lys Arg Val Ala Phe Gly Ser Ala His Gly Leu Glu Tyr Leu355 360 365His Glu His Cys Asn Pro Lys Ile Ile His Arg Asp Leu Lys Ala Ala370 375 380Asn Ile Leu Leu Asp Asn Asn Phe Glu Pro Val Leu Gly Asp Phe Gly385 390 395 400Leu Ala Lys Leu Val Asp Thr Ser Leu Thr His Val Thr Thr Gln Val405 410 415Arg Gly Thr Met Gly His Ile Ala Pro Glu Tyr Leu Cys Thr Gly Lys420 425 430Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Ile Thr Leu Leu Glu435 440 445Leu Val Thr Gly Gln Arg Ala Ile Asp Phe Ser Arg Leu Glu Glu Glu450 455 460Glu Asn Ile Leu Leu Leu Asp His Ile Lys Lys Leu Leu Arg Glu Gln465 470 475 480Arg Leu Arg Asp Ile Val Asp Ser Asn Leu Thr Thr Tyr Asp Ser Lys485 490 495Glu Val Glu Thr Ile Val Gln Val Ala Leu Leu Cys Thr Gln Gly Ser500 505 510Pro Glu Asp Arg Pro Ala Met Ser Glu Val Val Lys Met Leu Gln Gly515 520 525Thr Gly Gly Leu Ala Glu Lys Trp Thr Glu Trp Glu Gln Leu Glu Glu530 535 540Val Arg Asn Lys Glu Ala Leu Leu Leu Pro Thr Leu Pro Ala Thr Trp545 550 555 560Asp Glu Glu Glu Thr Thr Val Asp Gln Glu Ser Ile Arg Leu Ser Thr565 570 575Ala Arg26633PRTArabidopsis thaliana/note="Predicted protein domain RKS-4 of the RKS subfamily II" 26Met Val Val Met Lys Leu Ile Thr Met Lys Ile Phe Ser Val Leu Leu1 5 10 15Leu Leu Cys Phe Phe Val Thr Cys Ser Leu Ser Ser Glu Pro Arg Asn20 25 30Pro Glu Val Glu Ala Leu Ile Asn Ile Lys Asn Glu Leu His Asp Pro35 40 45His Gly Val Phe Lys Asn Trp Asp Glu Phe Ser Val Asp Pro Cys Ser50 55 60Trp Thr Met Ile Ser Cys Ser Ser Asp Asn Leu Val Ile Gly Leu Gly65 70 75 80Ala Pro Ser Gln Ser Leu Ser Gly Thr Leu Ser Gly Ser Ile Gly Asn85 90 95Leu Thr Asn Leu Arg Gln Val Ser Leu Gln Asn Asn Asn Ile Ser Gly100 105 110Lys Ile Pro Pro Glu Ile Cys Ser Leu Pro Lys Leu Gln Thr Leu Asp115 120 125Leu Ser Asn Asn Arg Phe Ser Gly Glu Ile Pro Gly Ser Val Asn Gln130 135 140Leu Ser Asn Leu Gln Tyr Leu Arg Leu Asn Asn Asn Ser Leu Ser Gly145 150 155 160Pro Phe Pro Ala Ser Leu Ser Gln Ile Pro His Leu Ser Phe Leu Asp165 170 175Leu Ser Tyr Asn Asn Leu Arg Gly Pro Val Pro Lys Phe Pro Ala Arg180 185 190Thr Phe Asn Val Ala Gly Asn Pro Leu Ile Cys Lys Asn Ser Leu Pro195 200 205Glu Ile Cys Ser Gly Ser Ile Ser Ala Ser Pro Leu Ser Val Ser Leu210 215 220Arg Ser Ser Ser Gly Arg Arg Asn Ile Leu Ala Val Ala Leu Gly Val225 230 235 240Ser Leu Gly Phe Ala Val Ser Val Ile Leu Ser Leu Gly Phe Ile Trp245 250 255Tyr Arg Lys Lys Gln Arg Arg Leu Thr Met Leu Arg Ile Ser Asp Lys260 265 270Gln Glu Glu Gly Leu Leu Gly Leu Gly Asn Leu Arg Ser Phe Thr Phe275 280 285Arg Glu Leu His Val Ala Thr Asp Gly Phe Ser Ser Lys Ser Ile Leu290 295 300Gly Ala Gly Gly Phe Gly Asn Val Tyr Arg Gly Lys Phe Gly Asp Gly305 310 315 320Thr Val Val Ala Val Lys Arg Leu Lys Asp Val Asn Gly Thr Ser Gly325 330 335Asn Ser Gln Phe Arg Thr Glu Leu Glu Met Ile Ser Leu Ala Val His340 345 350Arg Asn Leu Leu Arg Leu Ile Gly Tyr Cys Ala Ser Ser Ser Glu Arg355 360 365Leu Leu Val Tyr Pro Tyr Met Ser Asn Gly Ser Val Ala Ser Arg Leu370 375 380Lys Ala Lys Pro Ala Leu Asp Trp Asn Thr Arg Lys Lys Ile Ala Ile385 390 395 400Gly Ala Ala Arg Gly Leu Phe Tyr Leu His Glu Gln Cys Asp Pro

Lys405 410 415Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile Leu Leu Asp Glu Tyr420 425 430Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala Lys Leu Leu Asn His435 440 445Glu Asp Ser His Val Thr Thr Ala Val Arg Gly Thr Val Gly His Ile450 455 460Ala Pro Glu Tyr Leu Ser Thr Gly Gln Ser Ser Glu Lys Thr Asp Val465 470 475 480Phe Gly Phe Gly Ile Leu Leu Leu Glu Leu Ile Thr Gly Met Arg Ala485 490 495Leu Glu Phe Gly Lys Ser Val Ser Gln Lys Gly Ala Met Leu Glu Trp500 505 510Val Arg Lys Leu His Lys Glu Met Lys Val Glu Glu Leu Val Asp Arg515 520 525Glu Leu Gly Thr Thr Tyr Asp Arg Ile Glu Val Gly Glu Met Leu Gln530 535 540Val Ala Leu Leu Cys Thr Gln Phe Leu Pro Ala His Arg Pro Lys Met545 550 555 560Ser Glu Val Val Gln Met Leu Glu Gly Asp Gly Leu Ala Glu Arg Trp565 570 575Ala Ala Ser His Asp His Ser His Phe Tyr His Ala Asn Met Ser Tyr580 585 590Arg Thr Ile Thr Ser Thr Asp Gly Asn Asn Gln Thr Lys His Leu Phe595 600 605Gly Ser Ser Gly Phe Glu Asp Glu Asp Asp Asn Gln Ala Leu Asp Ser610 615 620Phe Ala Met Glu Leu Ser Gly Pro Arg625 63027588PRTArabidopsis thaliana/note="Predicted protein domain RKS-1 of the RKS subfamily II" 27Met Glu Gly Val Arg Phe Val Val Trp Arg Leu Gly Phe Leu Val Phe1 5 10 15Val Trp Phe Phe Asp Ile Ser Ser Ala Thr Leu Ser Pro Thr Gly Val20 25 30Asn Tyr Glu Val Thr Ala Leu Val Ala Val Lys Asn Glu Leu Asn Asp35 40 45Pro Tyr Lys Val Leu Glu Asn Trp Asp Val Asn Ser Val Asp Pro Cys50 55 60Ser Trp Arg Met Val Ser Cys Thr Asp Gly Tyr Val Ser Ser Leu Val65 70 75 80Leu Gln Asn Asn Ala Ile Thr Gly Pro Ile Pro Glu Thr Ile Gly Arg85 90 95Leu Glu Lys Leu Gln Ser Leu Asp Leu Ser Asn Asn Ser Phe Thr Gly100 105 110Glu Ile Pro Ala Ser Leu Gly Glu Leu Lys Asn Leu Asn Tyr Leu Arg115 120 125Leu Asn Asn Asn Ser Leu Ile Gly Thr Cys Pro Glu Ser Leu Ser Lys130 135 140Ile Glu Gly Leu Thr Leu Val Val Ile Gly Asn Ala Leu Ile Cys Gly145 150 155 160Pro Lys Ala Val Ser Asn Cys Ser Ala Val Pro Glu Pro Leu Thr Leu165 170 175Pro Gln Asp Gly Pro Asp Glu Ser Gly Thr Arg Thr Asn Gly His His180 185 190Val Ala Leu Ala Phe Ala Ala Ser Phe Ser Ala Ala Phe Phe Val Phe195 200 205Phe Thr Ser Gly Met Phe Leu Trp Trp Arg Tyr Arg Arg Asn Lys Gln210 215 220Ile Phe Phe Asp Val Asn Glu Gln Tyr Asp Pro Glu Val Ser Leu Gly225 230 235 240His Leu Lys Arg Tyr Thr Phe Lys Glu Leu Arg Ser Ala Thr Asn His245 250 255Phe Asn Ser Lys Asn Ile Leu Gly Arg Gly Gly Tyr Gly Ile Val Tyr260 265 270Lys Gly His Leu Asn Asp Gly Thr Leu Val Ala Val Lys Arg Leu Lys275 280 285Asp Cys Asn Ile Ala Gly Gly Glu Val Gln Phe Gln Thr Glu Val Glu290 295 300Thr Ile Ser Leu Ala Leu His Arg Asn Leu Leu Arg Leu Arg Gly Phe305 310 315 320Cys Ser Ser Asn Gln Glu Arg Ile Leu Val Tyr Pro Tyr Met Pro Asn325 330 335Gly Ser Val Ala Ser Arg Leu Lys Asp Asn Ile Arg Gly Glu Pro Ala340 345 350Leu Asp Trp Ser Arg Arg Lys Lys Ile Ala Val Gly Thr Ala Arg Gly355 360 365Leu Val Tyr Leu His Glu Gln Cys Asp Pro Lys Ile Ile His Arg Asp370 375 380Val Lys Ala Ala Asn Ile Leu Leu Asp Glu Asp Phe Glu Ala Val Val385 390 395 400Gly Asp Phe Gly Leu Ala Lys Leu Leu Asp His Arg Asp Ser His Val405 410 415Thr Thr Ala Val Arg Gly Thr Val Gly His Ile Ala Pro Glu Tyr Leu420 425 430Ser Thr Gly Gln Ser Ser Glu Lys Thr Asp Val Phe Gly Phe Gly Ile435 440 445Leu Leu Leu Glu Leu Ile Thr Gly Gln Lys Ala Leu Asp Phe Gly Arg450 455 460Ser Ala His Gln Lys Gly Val Met Leu Asp Trp Val Lys Lys Leu His465 470 475 480Gln Glu Gly Lys Leu Lys Gln Leu Ile Asp Lys Asp Leu Asn Asp Lys485 490 495Phe Asp Arg Val Glu Leu Glu Glu Ile Val Gln Val Ala Leu Leu Cys500 505 510Thr Gln Phe Asn Pro Ser His Arg Pro Lys Met Ser Glu Val Met Lys515 520 525Met Leu Glu Gly Asp Gly Leu Ala Glu Arg Trp Glu Ala Thr Gln Asn530 535 540Gly Thr Gly Glu His Gln Pro Pro Pro Leu Pro Pro Gly Met Val Ser545 550 555 560Ser Ser Pro Arg Val Arg Tyr Tyr Ser Asp Tyr Ile Gln Glu Ser Ser565 570 575Leu Val Val Glu Ala Ile Glu Leu Ser Gly Pro Arg580 58528634PRTArabidopsis thaliana/note="Predicted protein domain RKS-5 of the RKS subfamily II" 28Met Glu Ile Ser Leu Met Lys Phe Leu Phe Leu Gly Ile Trp Val Tyr1 5 10 15Tyr Tyr Ser Val Leu Asp Ser Val Ser Ala Met Asp Ser Leu Leu Ser20 25 30Pro Lys Trp Ala Ala Leu Met Ser Val Lys Asn Lys Met Lys Asp Glu35 40 45Lys Glu Val Leu Ser Gly Trp Asp Ile Asn Ser Val Asp Pro Cys Thr50 55 60Trp Asn Met Val Gly Cys Ser Ser Glu Gly Phe Val Val Ser Leu Leu65 70 75 80Leu Gln Asn Asn Gln Leu Thr Gly Pro Ile Pro Ser Glu Leu Gly Gln85 90 95Leu Ser Glu Leu Glu Thr Leu Asp Leu Ser Gly Asn Arg Phe Ser Gly100 105 110Glu Ile Pro Ala Ser Leu Gly Phe Leu Thr His Leu Asn Tyr Leu Arg115 120 125Leu Ser Arg Asn Leu Leu Ser Gly Gln Val Pro His Leu Val Ala Gly130 135 140Leu Ser Gly Leu Ser Phe Leu Asp Leu Ser Phe Asn Asn Leu Ser Gly145 150 155 160Pro Thr Pro Asn Ile Ser Ala Lys Asp Tyr Arg Ile Val Gly Asn Ala165 170 175Phe Leu Cys Gly Pro Ala Ser Gln Glu Leu Cys Ser Asp Ala Thr Pro180 185 190Val Arg Asn Gly Met Leu Leu Arg Lys Phe Phe Ala Lys Leu Tyr Leu195 200 205Lys His Gly Phe Val Tyr Leu Thr Ser Cys Asn Arg Ser Ala Ala Thr210 215 220Gly Leu Ser Glu Lys Asp Asn Ser Lys His His Ser Leu Val Leu Ser225 230 235 240Phe Ala Phe Gly Ile Val Val Ala Phe Ile Ile Ser Leu Met Phe Leu245 250 255Phe Phe Trp Val Leu Trp His Arg Ser Arg Leu Ser Arg Ser His Gly260 265 270Thr Tyr Leu Ile Val Ser Leu Cys Leu Ser Tyr Thr Ile Tyr Val Lys275 280 285Thr Leu Leu Lys Ser Ala Leu Leu Phe Met Asp Phe Leu Val Gln Gln290 295 300Asp Tyr Glu Phe Glu Ile Gly His Leu Lys Arg Phe Ser Phe Arg Glu305 310 315 320Ile Gln Thr Ala Thr Ser Asn Phe Ser Pro Lys Asn Ile Leu Gly Gln325 330 335Gly Gly Phe Gly Met Val Tyr Lys Gly Tyr Leu Pro Asn Gly Thr Val340 345 350Val Ala Val Lys Arg Leu Lys Asp Pro Ile Tyr Thr Gly Glu Val Gln355 360 365Phe Gln Thr Glu Val Glu Met Ile Gly Leu Ala Val His Arg Asn Leu370 375 380Leu Arg Leu Phe Gly Phe Cys Met Thr Pro Glu Glu Arg Met Leu Val385 390 395 400Tyr Pro Tyr Met Pro Asn Gly Ser Val Ala Asp Arg Leu Arg Asp Trp405 410 415Asn Arg Arg Ile Ser Ile Ala Leu Gly Ala Ala Arg Gly Leu Val Tyr420 425 430Leu His Glu Gln Cys Asn Pro Lys Ile Ile His Arg Asp Val Lys Ala435 440 445Ala Asn Ile Leu Leu Asp Glu Ser Phe Glu Ala Ile Val Gly Asp Phe450 455 460Gly Leu Ala Lys Leu Leu Asp Gln Arg Asp Ser His Val Thr Thr Ala465 470 475 480Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly485 490 495Gln Ser Ser Glu Lys Thr Asp Val Phe Gly Phe Gly Val Leu Ile Leu500 505 510Glu Leu Ile Thr Gly His Lys Met Ile Asp Gln Gly Asn Gly Gln Val515 520 525Arg Lys Gly Met Ile Leu Ser Trp Val Arg Thr Leu Lys Ala Glu Lys530 535 540Arg Phe Ala Glu Met Val Asp Arg Asp Leu Lys Gly Glu Phe Asp Asp545 550 555 560Leu Val Leu Glu Glu Val Val Glu Leu Ala Leu Leu Cys Thr Gln Pro565 570 575His Pro Asn Leu Arg Pro Arg Met Ser Gln Val Leu Lys Val Leu Glu580 585 590Gly Leu Val Glu Gln Cys Glu Gly Gly Tyr Glu Ala Arg Ala Pro Ala595 600 605Ser Val Ser Arg Asn Tyr Ser Asn Gly His Glu Glu Gln Ser Phe Ile610 615 620Ile Glu Ala Ile Glu Leu Ser Gly Pro Arg625 63029603PRTArabidopsis thaliana/note="Predicted protein domain RK-7 of the RKS subfamily II" 29Met Leu Gln Gly Arg Arg Glu Ala Lys Lys Ser Tyr Ala Leu Phe Ser1 5 10 15Ser Thr Phe Phe Phe Phe Phe Ile Cys Phe Leu Ser Ser Ser Ser Ala20 25 30Glu Leu Thr Asp Lys Val Val Ala Leu Ile Gly Ile Lys Ser Ser Leu35 40 45Thr Asp Pro His Gly Val Leu Met Asn Trp Asp Asp Thr Ala Val Asp50 55 60Pro Cys Ser Trp Asn Met Ile Thr Cys Ser Asp Gly Phe Val Ile Arg65 70 75 80Leu Tyr Arg Leu Leu Gln Asn Asn Tyr Ile Thr Gly Asn Ile Pro His85 90 95Glu Ile Gly Lys Leu Met Lys Leu Lys Thr Leu Asp Leu Ser Thr Asn100 105 110Asn Pro Thr Gly Gln Ile Pro Phe Thr Leu Ser Tyr Ser Lys Asn Leu115 120 125His Arg Arg Val Asn Asn Asn Ser Leu Thr Gly Thr Ile Pro Ser Ser130 135 140Leu Ala Asn Met Thr Gln Leu Thr Phe Leu Leu Asp Leu Ser Tyr Asn145 150 155 160Asn Leu Ser Gly Pro Val Pro Arg Ser Leu Ala Lys Thr Phe Asn Val165 170 175Met Gly Asn Ser Gln Ile Cys Pro Thr Gly Thr Glu Lys Asp Cys Asn180 185 190Gly Thr Gln Pro Lys Pro Met Ser Ile Thr Leu Asn Ser Ser Gln Arg195 200 205Gly Thr Lys Asn Arg Lys Ile Ala Val Val Phe Gly Val Ser Leu Thr210 215 220Cys Val Cys Leu Leu Ile Ile Gly Phe Gly Phe Leu Leu Trp Trp Arg225 230 235 240Arg Arg His Asn Lys Gln Val Leu Phe Phe Asp Ile Asn Glu Gln Asn245 250 255Lys Glu Glu Met Cys Leu Gly Asn Leu Arg Arg Phe Asn Phe Lys Glu260 265 270Leu Gln Ser Ala Thr Ser Asn Phe Ser Ser Lys Asn Leu Val Gly Lys275 280 285Gly Gly Phe Gly Asn Val Tyr Lys Gly Cys Leu His Asp Gly Ser Ile290 295 300Ile Ala Val Lys Arg Leu Lys Asp Ile Asn Asn Gly Gly Gly Glu Val305 310 315 320Gln Phe Gln Thr Glu Leu Glu Met Ile Ser Leu Ala Val Glu Arg Asn325 330 335Leu Leu Arg Leu Tyr Gly Phe Cys Thr Thr Ser Ser Glu Arg Leu Leu340 345 350Val Tyr Pro Tyr Met Ser Asn Gly Ser Val Ala Ser Arg Leu Lys Ala355 360 365Lys Pro Val Leu Asp Trp Gly Thr Arg Lys Arg Ile Ala Leu Gly Ala370 375 380Gly Arg Gly Leu Leu Tyr Leu His Glu Gln Cys Asp Pro Lys Ile Ile385 390 395 400His Arg Asp Val Lys Ala Ala Asn Ile Leu Leu Asp Asp Tyr Phe Glu405 410 415Ala Val Val Gly Asp Phe Gly Leu Ala Lys Leu Leu Asp His Glu Glu420 425 430Ser His Val Thr Thr Ala Val Arg Gly Thr Val Gly His Ile Ala Pro435 440 445Glu Tyr Leu Ser Thr Gly Gln Ser Ser Glu Lys Thr Asp Val Phe Gly450 455 460Phe Gly Ile Leu Leu Leu Glu Leu Ile Thr Gly Leu Arg Ala Leu Glu465 470 475 480Phe Gly Lys Ala Ala Asn Gln Arg Gly Ala Ile Leu Asp Trp Val Lys485 490 495Lys Leu Gln Gln Glu Lys Lys Leu Glu Gln Ile Val Asp Lys Asp Leu500 505 510Lys Ser Asn Tyr Asp Arg Ile Glu Val Glu Glu Met Val Gln Val Ala515 520 525Leu Leu Cys Thr Gln Tyr Leu Pro Ile His Arg Pro Lys Met Ser Glu530 535 540Val Val Arg Met Leu Glu Gly Asp Gly Leu Val Glu Lys Trp Glu Ala545 550 555 560Ser Ser Gln Arg Ala Glu Thr Asn Arg Ser Tyr Ser Lys Pro Asn Glu565 570 575Phe Ser Ser Ser Glu Arg Tyr Ser Asp Leu Thr Asp Asp Ser Ser Val580 585 590Leu Val Gln Ala Met Glu Leu Ser Gly Pro Arg595 60030628PRTArabidopsis thaliana/note="Predicted protein domain RKS-8 of the RKS subfamily III" 30Met Gly Arg Lys Lys Phe Glu Ala Phe Gly Phe Val Cys Leu Ile Ser1 5 10 15Leu Leu Leu Leu Phe Asn Ser Leu Trp Leu Ala Ser Ser Asn Met Glu20 25 30Gly Asp Ala Leu His Ser Leu Arg Ala Asn Leu Val Asp Pro Asn Asn35 40 45Val Leu Gln Ser Trp Asp Pro Thr Leu Val Asn Pro Cys Thr Trp Phe50 55 60His Val Thr Cys Asn Asn Glu Asn Ser Val Ile Arg Val Asp Leu Gly65 70 75 80Asn Ala Asp Leu Ser Gly Gln Leu Val Pro Gln Leu Gly Gln Leu Lys85 90 95Asn Leu Gln Tyr Leu Glu Leu Tyr Ser Asn Asn Ile Thr Gly Pro Val100 105 110Pro Ser Asp Leu Gly Asn Leu Thr Asn Leu Val Ser Leu Asp Leu Tyr115 120 125Leu Asn Ser Phe Thr Gly Pro Ile Pro Asp Ser Leu Gly Lys Leu Phe130 135 140Lys Leu Arg Phe Leu Arg Leu Asn Asn Asn Ser Leu Thr Gly Pro Ile145 150 155 160Pro Met Ser Leu Thr Asn Ile Met Thr Leu Gln Val Leu Asp Leu Ser165 170 175Asn Asn Arg Leu Ser Gly Ser Val Pro Asp Asn Gly Ser Phe Ser Leu180 185 190Phe Thr Pro Ile Ser Phe Ala Asn Asn Leu Asp Leu Cys Gly Pro Val195 200 205Thr Leu Arg Pro Cys Pro Gly Ser Pro Pro Phe Ser Pro Pro Pro Pro210 215 220Phe Ile Pro Pro Pro Ile Val Pro Thr Pro Gly Gly Tyr Ser Ala Thr225 230 235 240Gly Ala Ile Ala Gly Gly Val Ala Ala Gly Ala Ala Leu Leu Phe Ala245 250 255Ala Pro Ala Leu Ala Phe Ala Trp Trp Arg Arg Arg Lys Pro Gln Glu260 265 270Phe Phe Phe Asp Val Pro Ala Glu Glu Asp Pro Glu Val His Leu Gly275 280 285Gln Leu Lys Arg Phe Ser Leu Arg Glu Leu Gln Val Ala Thr Asp Ser290 295 300Phe Ser Asn Lys Asn Ile Leu Gly Arg Gly Gly Phe Gly Lys Val Tyr305 310 315 320Lys Gly Arg Leu Ala Asp Gly Thr Leu Val Ala Val Lys Arg Leu Lys325 330 335Glu Glu Arg Thr Pro Gly Gly Glu Leu Gln Phe Gln Thr Glu Val Glu340 345 350Met Ile Ser Met Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly Phe355 360 365Cys Met Thr Pro Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn370 375 380Gly Ser Val Ala Ser Cys Leu Arg Glu Arg Pro Pro Ser Gln Leu Pro385 390 395 400Leu Ala Trp Ser Ile Arg Gln Gln Ile Ala Leu Gly Ser Ala Arg Gly405 410 415Leu Ser Tyr Leu His Asp His Cys Asp Pro Lys Ile Ile His Arg Asp420 425 430Val Lys Ala Ala Asn Ile Leu Leu Asp Glu Glu Phe Glu Ala Val Val435 440 445Gly Asp Phe Gly Leu Ala Arg Leu Met Asp Tyr Lys Asp Thr His Val450 455 460Thr Thr Ala Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu465 470 475 480Ser Thr Gly Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Ile485 490 495Met Leu Leu Glu Leu Ile Thr Gly Gln Arg Ala Phe Asp Leu Ala Arg500 505 510Leu Ala Asn Asp Asp Asp Val Met Leu Leu Asp Trp Val Lys Gly Leu515 520 525Leu Lys Glu Lys Lys Leu Glu Met Leu Val Asp Pro Asp Leu Gln Ser530 535 540Asn Tyr Thr Glu Ala Glu Val Glu Gln Leu Ile Gln Val Ala Leu Leu545 550 555 560Cys

Thr Gln Ser Ser Pro Met Glu Arg Pro Lys Met Ser Glu Val Val565 570 575Arg Met Leu Glu Gly Asp Gly Leu Ala Glu Lys Trp Asp Glu Trp Gln580 585 590Lys Val Glu Val Leu Arg Gln Glu Val Glu Leu Ser Ser His Pro Thr595 600 605Ser Asp Trp Ile Leu Asp Ser Thr Asp Asn Leu His Ala Met Glu Leu610 615 620Ser Gly Pro Arg62531591PRTArabidopsis thaliana/note="Predicted protein domain RKS-10 of the RKS subfamily III" 31Met Glu Arg Arg Leu Met Ile Pro Cys Phe Phe Trp Leu Ile Leu Val1 5 10 15Leu Asp Leu Val Leu Arg Val Ser Gly Asn Ala Glu Gly Asp Ala Leu20 25 30Ser Ala Leu Lys Asn Ser Leu Ala Asp Pro Asn Lys Val Leu Gln Ser35 40 45Trp Asp Ala Thr Leu Val Thr Pro Cys Thr Trp Phe His Val Thr Cys50 55 60Asn Ser Asp Asn Ser Val Thr Arg Val Asp Leu Gly Asn Ala Asn Leu65 70 75 80Ser Gly Gln Leu Val Met Gln Leu Gly Gln Leu Pro Asn Leu Gln Tyr85 90 95Leu Glu Leu Tyr Ser Asn Asn Ile Thr Gly Thr Ile Pro Glu Gln Leu100 105 110Gly Asn Leu Thr Glu Leu Val Ser Leu Asp Leu Tyr Leu Asn Asn Leu115 120 125Ser Gly Pro Ile Pro Ser Thr Leu Gly Arg Leu Lys Lys Leu Arg Phe130 135 140Leu Arg Leu Asn Asn Asn Ser Leu Ser Gly Glu Ile Pro Arg Ser Leu145 150 155 160Thr Ala Val Leu Thr Leu Gln Val Leu Phe Ala Asn Thr Lys Leu Thr165 170 175Pro Leu Pro Ala Ser Pro Pro Pro Pro Ile Ser Pro Thr Pro Pro Ser180 185 190Pro Ala Gly Ser Asn Arg Ile Thr Gly Ala Ile Ala Gly Gly Val Ala195 200 205Ala Gly Ala Ala Leu Leu Phe Ala Val Pro Ala Ile Ala Leu Ala Trp210 215 220Trp Arg Arg Lys Lys Pro Gln Asp His Phe Phe Asp Val Pro Ala Glu225 230 235 240Glu Asp Pro Glu Val His Leu Gly Gln Leu Lys Arg Phe Ser Leu Arg245 250 255Glu Leu Gln Val Ala Ser Asp Asn Phe Ser Asn Lys Asn Ile Leu Gly260 265 270Arg Gly Gly Phe Gly Lys Val Tyr Lys Gly Arg Leu Ala Asp Gly Thr275 280 285Leu Val Ala Val Lys Arg Leu Lys Glu Glu Arg Thr Gln Gly Gly Glu290 295 300Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser Met Ala Val His Arg305 310 315 320Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr Pro Thr Glu Arg Leu325 330 335Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val Ala Ser Cys Leu Arg340 345 350Glu Arg Pro Glu Ser Gln Pro Pro Leu Asp Trp Pro Lys Arg Gln Arg355 360 365Ile Ala Leu Gly Ser Ala Arg Gly Leu Ala Tyr Leu His Asp His Cys370 375 380Asp Pro Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile Leu Leu385 390 395 400Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala Lys Leu405 410 415Met Asp Tyr Lys Asp Thr His Val Thr Thr Ala Val Arg Gly Thr Ile420 425 430Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Lys Ser Ser Glu Lys435 440 445Thr Asp Val Phe Gly Tyr Gly Val Met Leu Leu Glu Leu Ile Thr Gly450 455 460Gln Arg Ala Phe Asp Leu Ala Arg Leu Ala Asn Asp Asp Asp Val Met465 470 475 480Leu Leu Asp Trp Val Lys Gly Leu Leu Lys Glu Lys Lys Leu Glu Ala485 490 495Leu Val Asp Val Asp Leu Gln Gly Asn Tyr Lys Asp Glu Glu Val Glu500 505 510Gln Leu Ile Gln Val Ala Leu Leu Cys Thr Gln Ser Ser Pro Met Glu515 520 525Arg Pro Lys Met Ser Glu Val Val Arg Met Leu Glu Gly Asp Gly Leu530 535 540Ala Glu Arg Trp Glu Glu Trp Gln Lys Glu Glu Met Phe Arg Gln Asp545 550 555 560Phe Asn Tyr Pro Thr His His Pro Ala Val Ser Gly Trp Ile Ile Gly565 570 575Asp Ser Thr Ser Gln Ile Glu Asn Glu Tyr Pro Ser Gly Pro Arg580 585 59032579PRTArabidopsis thaliana/note="Predicted protein domain RKS-12 of the RKS subfamily III" 32Met Glu His Gly Ser Ser Arg Gly Phe Ile Trp Leu Ile Leu Phe Leu1 5 10 15Asp Phe Val Ser Arg Val Thr Gly Lys Thr Gln Val Asp Ala Leu Ile20 25 30Ala Leu Arg Ser Ser Leu Ser Ser Gly Asp His Thr Asn Asn Ile Leu35 40 45Gln Ser Trp Asn Ala Thr His Val Thr Pro Cys Ser Trp Phe His Val50 55 60Thr Cys Asn Thr Glu Asn Ser Val Thr Arg Leu Glu Leu Phe Asn Asn65 70 75 80Asn Ile Thr Gly Glu Ile Pro Glu Glu Leu Gly Asp Leu Met Glu Leu85 90 95Val Ser Leu Asp Leu Phe Ala Asn Asn Ile Ser Gly Pro Ile Pro Ser100 105 110Ser Leu Gly Lys Leu Gly Lys Leu Arg Phe Leu Arg Leu Tyr Asn Asn115 120 125Ser Leu Ser Gly Glu Ile Pro Arg Ser Leu Thr Ala Leu Pro Leu Asp130 135 140Val Leu Asp Ile Ser Asn Asn Arg Leu Ser Gly Asp Ile Pro Val Asn145 150 155 160Gly Ser Phe Ser Gln Phe Thr Ser Met Arg Phe Ala Asn Asn Lys Leu165 170 175Arg Pro Arg Pro Ala Ser Pro Ser Pro Ser Pro Ser Gly Gly Thr Ser180 185 190Ala Ala Ile Val Val Gly Val Ala Ala Gly Ala Ala Leu Leu Phe Ala195 200 205Leu Ala Trp Trp Leu Arg Arg Lys Leu Gln Gly His Phe Leu Asp Val210 215 220Pro Ala Ala Glu Glu Asp Pro Glu Val Tyr Leu Gly Gln Phe Lys Arg225 230 235 240Phe Ser Leu Arg Glu Leu Leu Val Ala Thr Glu Lys Phe Ser Lys Arg245 250 255Asn Val Leu Gly Lys Gly Arg Phe Gly Ile Leu Tyr Lys Gly Arg Leu260 265 270Ala Asp Asp Thr Leu Val Ala Val Lys Arg Leu Asn Glu Glu Arg Thr275 280 285Lys Gly Gly Glu Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser Met290 295 300Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr Pro305 310 315 320Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val Ala325 330 335Ser Cys Leu Arg Glu Arg Pro Glu Gly Asn Pro Ala Leu Asp Trp Pro340 345 350Lys Arg Lys His Ile Ala Leu Gly Ser Ala Arg Gly Leu Ala Tyr Leu355 360 365His Asp His Cys Asp Gln Lys Ile Ile His Leu Asp Val Lys Ala Ala370 375 380Asn Ile Leu Leu Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe Gly385 390 395 400Leu Ala Lys Leu Met Asn Tyr Asn Asp Ser His Val Thr Thr Ala Val405 410 415Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Lys420 425 430Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Val Met Leu Leu Glu435 440 445Leu Ile Thr Gly Gln Lys Ala Phe Asp Leu Ala Arg Leu Ala Asn Asp450 455 460Asp Asp Ile Met Leu Leu Asp Trp Val Lys Glu Val Leu Lys Glu Lys465 470 475 480Lys Leu Glu Ser Leu Val Asp Ala Glu Leu Glu Gly Lys Tyr Val Glu485 490 495Thr Glu Val Glu Gln Leu Ile Gln Met Ala Leu Leu Cys Thr Gln Ser500 505 510Ser Ala Met Glu Arg Pro Lys Met Ser Glu Val Val Arg Met Leu Glu515 520 525Gly Asp Gly Leu Ala Glu Arg Trp Glu Glu Trp Gln Lys Glu Glu Met530 535 540Pro Ile His Asp Phe Asn Tyr Gln Ala Tyr Pro His Ala Gly Thr Asp545 550 555 560Trp Leu Ile Pro Tyr Ser Asn Ser Leu Ile Glu Asn Asp Tyr Pro Ser565 570 575Gly Pro Arg33592PRTArabidopsis thaliana/note="Predicted protein domain RKS-13 of the RKS subfamily III" 33Met Glu Gln Arg Ser Leu Leu Cys Phe Leu Tyr Leu Leu Leu Leu Phe1 5 10 15Asn Phe Thr Leu Arg Val Ala Gly Asn Ala Glu Gly Asp Ala Leu Thr20 25 30Gln Leu Lys Asn Ser Leu Ser Ser Gly Asp Pro Ala Asn Asn Val Leu35 40 45Gln Ser Trp Asp Ala Thr Leu Val Thr Pro Cys Thr Trp Phe His Val50 55 60Thr Cys Asn Pro Glu Asn Lys Val Thr Arg Val Glu Leu Tyr Ser Asn65 70 75 80Asn Ile Thr Gly Glu Ile Pro Glu Glu Leu Gly Asp Leu Val Glu Leu85 90 95Val Ser Leu Asp Leu Tyr Ala Asn Ser Ile Ser Gly Pro Ile Pro Ser100 105 110Ser Leu Gly Lys Leu Gly Lys Leu Arg Phe Leu Arg Leu Asn Asn Asn115 120 125Ser Leu Ser Gly Glu Ile Pro Met Thr Leu Thr Ser Val Gln Leu Gln130 135 140Val Leu Asp Ile Ser Asn Asn Arg Leu Ser Gly Asp Ile Pro Val Asn145 150 155 160Gly Ser Phe Ser Leu Phe Thr Pro Ile Ser Phe Ala Asn Asn Ser Leu165 170 175Thr Asp Leu Pro Glu Pro Pro Pro Thr Ser Thr Ser Pro Thr Pro Pro180 185 190Pro Pro Ser Gly Gly Gln Met Thr Ala Ala Ile Ala Gly Gly Val Ala195 200 205Ala Gly Ala Ala Leu Leu Phe Ala Val Pro Ala Ile Ala Phe Ala Trp210 215 220Trp Leu Arg Arg Lys Pro Gln Asp His Phe Phe Asp Val Pro Gly Ala225 230 235 240Glu Glu Asp Pro Glu Val His Leu Gly Gln Leu Lys Arg Phe Thr Leu245 250 255Arg Glu Leu Leu Val Ala Thr Asp Asn Phe Ser Asn Lys Asn Val Leu260 265 270Gly Arg Gly Gly Phe Gly Lys Val Tyr Lys Gly Arg Leu Ala Asp Gly275 280 285Asn Leu Val Ala Val Lys Arg Leu Lys Glu Glu Arg Thr Lys Gly Gly290 295 300Glu Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser Met Ala Val His305 310 315 320Arg Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr Pro Thr Glu Arg325 330 335Leu Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val Ala Ser Cys Leu340 345 350Arg Glu Arg Pro Glu Gly Asn Pro Ala Leu Asp Trp Pro Lys Arg Lys355 360 365His Ile Ala Leu Gly Ser Ala Arg Gly Leu Ala Tyr Leu His Asp His370 375 380Cys Asp Gln Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile Leu385 390 395 400Leu Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala Lys405 410 415Leu Met Asn Tyr Asn Asp Ser His Val Thr Thr Ala Val Arg Gly Thr420 425 430Ile Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Lys Ser Ser Glu435 440 445Lys Thr Asp Val Phe Gly Tyr Gly Val Met Leu Leu Glu Leu Ile Thr450 455 460Gly Gln Lys Ala Phe Asp Leu Ala Arg Leu Ala Asn Asp Asp Asp Ile465 470 475 480Met Leu Leu Asp Trp Val Lys Glu Val Leu Lys Glu Lys Lys Leu Glu485 490 495Ser Leu Val Asp Ala Glu Leu Glu Gly Lys Tyr Val Glu Thr Glu Val500 505 510Glu Gln Leu Ile Gln Met Ala Leu Leu Cys Thr Gln Ser Ser Ala Met515 520 525Glu Arg Pro Lys Met Ser Glu Val Val Arg Met Leu Glu Gly Asp Gly530 535 540Leu Ala Glu Arg Trp Glu Glu Trp Gln Lys Glu Glu Met Pro Ile His545 550 555 560Asp Phe Asn Tyr Gln Ala Tyr Pro His Ala Gly Thr Asp Trp Leu Ile565 570 575Pro Tyr Ser Asn Ser Leu Ile Glu Asn Asp Tyr Pro Ser Gly Pro Arg580 585 590341794DNAArabidopsis thalianaCDS(28)..(1791)/note="Arabidopsis thaliana RKS1 cDNA" 34ccaaagttga ttgctttaag aagggat atg gaa ggt gtg aga ttt gtg gtg tgg 54Met Glu Gly Val Arg Phe Val Val Trp1 5aga tta gga ttt ctg gtt ttt gta tgg ttc ttt gat atc tct tct gct 102Arg Leu Gly Phe Leu Val Phe Val Trp Phe Phe Asp Ile Ser Ser Ala10 15 20 25aca ctt tct cct act ggt gta aac tat gaa gtg aca gct ttg gtt gct 150Thr Leu Ser Pro Thr Gly Val Asn Tyr Glu Val Thr Ala Leu Val Ala30 35 40gtg aag aat gaa ttg aat gat ccg tac aaa gtt ctt gag aat tgg gat 198Val Lys Asn Glu Leu Asn Asp Pro Tyr Lys Val Leu Glu Asn Trp Asp45 50 55gtg aat tca gtt gat cct tgt agc tgg aga atg gtt tct tgc act gat 246Val Asn Ser Val Asp Pro Cys Ser Trp Arg Met Val Ser Cys Thr Asp60 65 70ggc tat gtc tct tca ctg gtg ttg caa aac aat gca atc act ggt cca 294Gly Tyr Val Ser Ser Leu Val Leu Gln Asn Asn Ala Ile Thr Gly Pro75 80 85att ccg gaa acg att ggg agg ttg gag aag ctt cag tca ctt gat ctt 342Ile Pro Glu Thr Ile Gly Arg Leu Glu Lys Leu Gln Ser Leu Asp Leu90 95 100 105tcg aac aat tca ttc acc ggg gag ata ccg gcc tca ctt gga gaa ctc 390Ser Asn Asn Ser Phe Thr Gly Glu Ile Pro Ala Ser Leu Gly Glu Leu110 115 120aag aac ttg aat tac ttg cgg tta aac aat aac agt ctt ata gga act 438Lys Asn Leu Asn Tyr Leu Arg Leu Asn Asn Asn Ser Leu Ile Gly Thr125 130 135tgc cct gag tct cta tcc aag att gag gga ctc act cta gtg gta att 486Cys Pro Glu Ser Leu Ser Lys Ile Glu Gly Leu Thr Leu Val Val Ile140 145 150ggt aat gcg tta atc tgt ggc cca aaa gct gtt tca aac tgt tct gct 534Gly Asn Ala Leu Ile Cys Gly Pro Lys Ala Val Ser Asn Cys Ser Ala155 160 165gtt ccc gag cct ctc acg ctt cca caa gat ggt cca gat gaa tca gga 582Val Pro Glu Pro Leu Thr Leu Pro Gln Asp Gly Pro Asp Glu Ser Gly170 175 180 185act cgt acc aat ggc cat cac gtt gct ctt gca ttt gcc gca agc ttc 630Thr Arg Thr Asn Gly His His Val Ala Leu Ala Phe Ala Ala Ser Phe190 195 200agt gca gca ttt ttt gtt ttc ttt aca agc gga atg ttt ctt tgg tgg 678Ser Ala Ala Phe Phe Val Phe Phe Thr Ser Gly Met Phe Leu Trp Trp205 210 215aga tat cgc cgt aac aag caa ata ttt ttt gac gtt aat gaa caa tat 726Arg Tyr Arg Arg Asn Lys Gln Ile Phe Phe Asp Val Asn Glu Gln Tyr220 225 230gat cca gaa gtg agt tta ggg cac ttg aag agg tat aca ttc aaa gag 774Asp Pro Glu Val Ser Leu Gly His Leu Lys Arg Tyr Thr Phe Lys Glu235 240 245ctt aga tct gcc acc aat cat ttc aac tcg aag aac att ctc gga aga 822Leu Arg Ser Ala Thr Asn His Phe Asn Ser Lys Asn Ile Leu Gly Arg250 255 260 265ggc gga tac ggg att gtg tac aaa gga cac tta aac gat gga act ttg 870Gly Gly Tyr Gly Ile Val Tyr Lys Gly His Leu Asn Asp Gly Thr Leu270 275 280gtg gct gtc aaa cgt ctc aag gac tgt aac att gcg ggt gga gaa gtc 918Val Ala Val Lys Arg Leu Lys Asp Cys Asn Ile Ala Gly Gly Glu Val285 290 295cag ttt cag aca gaa gta gag act ata agt ttg gct ctt cat cgc aat 966Gln Phe Gln Thr Glu Val Glu Thr Ile Ser Leu Ala Leu His Arg Asn300 305 310ctc ctc cgg ctc cgc ggt ttc tgt agt agc aac cag gag aga att tta 1014Leu Leu Arg Leu Arg Gly Phe Cys Ser Ser Asn Gln Glu Arg Ile Leu315 320 325gtc tac cct tac atg cca aat ggg agt gtc gca tca cgc tta aaa gat 1062Val Tyr Pro Tyr Met Pro Asn Gly Ser Val Ala Ser Arg Leu Lys Asp330 335 340 345aat atc cgt gga gag cca gca tta gac tgg tcg aga agg aag aag ata 1110Asn Ile Arg Gly Glu Pro Ala Leu Asp Trp Ser Arg Arg Lys Lys Ile350 355 360gcg gtt ggg aca gcg aga gga cta gtt tac cta cac gag caa tgt gac 1158Ala Val Gly Thr Ala Arg Gly Leu Val Tyr Leu His Glu Gln Cys Asp365 370 375ccg aag att ata cac cgc gat gtg aaa gca gct aac att ctg tta gat 1206Pro Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile Leu Leu Asp380 385 390gag gac ttc gaa gca gtt gtt ggt gat ttt ggg tta gct aag ctt cta 1254Glu Asp Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala Lys Leu Leu395 400 405gac cat aga gac tct cat gtc aca act gca gtc cgt gga act gtt ggc 1302Asp His Arg Asp Ser His Val Thr Thr Ala Val Arg Gly Thr Val Gly410 415 420 425cac att gca cct gag tac tta tcc acg ggt cag tcc tca gag aag act 1350His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Gln Ser Ser Glu Lys Thr430 435 440gat gtc ttt ggc ttt ggc ata ctt ctc ctt gag ctc att act ggt cag 1398Asp Val Phe Gly Phe Gly Ile Leu Leu Leu Glu Leu Ile Thr Gly Gln445 450 455aaa gct ctt gat ttt ggc aga tcc gca cac cag aaa ggt gta atg ctt 1446Lys

Ala Leu Asp Phe Gly Arg Ser Ala His Gln Lys Gly Val Met Leu460 465 470gac tgg gtg aag aag ctg cac caa gaa ggg aaa cta aag cag tta ata 1494Asp Trp Val Lys Lys Leu His Gln Glu Gly Lys Leu Lys Gln Leu Ile475 480 485gac aaa gat cta aat gac aag ttc gat aga gta gaa ctc gaa gaa atc 1542Asp Lys Asp Leu Asn Asp Lys Phe Asp Arg Val Glu Leu Glu Glu Ile490 495 500 505gtt caa gtt gcg cta ctc tgc act caa ttc aat cca tct cat cga ccg 1590Val Gln Val Ala Leu Leu Cys Thr Gln Phe Asn Pro Ser His Arg Pro510 515 520aaa atg tca gaa gtt atg aag atg ctt gaa ggt gac ggt ttg gct gag 1638Lys Met Ser Glu Val Met Lys Met Leu Glu Gly Asp Gly Leu Ala Glu525 530 535aga tgg gaa gcg acg cag aac ggt act ggt gag cat cag cca ccg cca 1686Arg Trp Glu Ala Thr Gln Asn Gly Thr Gly Glu His Gln Pro Pro Pro540 545 550ttg cca ccg ggg atg gtg agt tct tcg ccg cgt gtg agg tat tac tcg 1734Leu Pro Pro Gly Met Val Ser Ser Ser Pro Arg Val Arg Tyr Tyr Ser555 560 565gat tat att cag gaa tcg tct ctt gta gta gaa gcc att gag ctc tcg 1782Asp Tyr Ile Gln Glu Ser Ser Leu Val Val Glu Ala Ile Glu Leu Ser570 575 580 585ggt cct cga tga 1794Gly Pro Arg35 588PRTArabidopsis thaliana 35Met Glu Gly Val Arg Phe Val Val Trp Arg Leu Gly Phe Leu Val Phe1 5 10 15Val Trp Phe Phe Asp Ile Ser Ser Ala Thr Leu Ser Pro Thr Gly Val20 25 30Asn Tyr Glu Val Thr Ala Leu Val Ala Val Lys Asn Glu Leu Asn Asp35 40 45Pro Tyr Lys Val Leu Glu Asn Trp Asp Val Asn Ser Val Asp Pro Cys50 55 60Ser Trp Arg Met Val Ser Cys Thr Asp Gly Tyr Val Ser Ser Leu Val65 70 75 80Leu Gln Asn Asn Ala Ile Thr Gly Pro Ile Pro Glu Thr Ile Gly Arg85 90 95Leu Glu Lys Leu Gln Ser Leu Asp Leu Ser Asn Asn Ser Phe Thr Gly100 105 110Glu Ile Pro Ala Ser Leu Gly Glu Leu Lys Asn Leu Asn Tyr Leu Arg115 120 125Leu Asn Asn Asn Ser Leu Ile Gly Thr Cys Pro Glu Ser Leu Ser Lys130 135 140Ile Glu Gly Leu Thr Leu Val Val Ile Gly Asn Ala Leu Ile Cys Gly145 150 155 160Pro Lys Ala Val Ser Asn Cys Ser Ala Val Pro Glu Pro Leu Thr Leu165 170 175Pro Gln Asp Gly Pro Asp Glu Ser Gly Thr Arg Thr Asn Gly His His180 185 190Val Ala Leu Ala Phe Ala Ala Ser Phe Ser Ala Ala Phe Phe Val Phe195 200 205Phe Thr Ser Gly Met Phe Leu Trp Trp Arg Tyr Arg Arg Asn Lys Gln210 215 220Ile Phe Phe Asp Val Asn Glu Gln Tyr Asp Pro Glu Val Ser Leu Gly225 230 235 240His Leu Lys Arg Tyr Thr Phe Lys Glu Leu Arg Ser Ala Thr Asn His245 250 255Phe Asn Ser Lys Asn Ile Leu Gly Arg Gly Gly Tyr Gly Ile Val Tyr260 265 270Lys Gly His Leu Asn Asp Gly Thr Leu Val Ala Val Lys Arg Leu Lys275 280 285Asp Cys Asn Ile Ala Gly Gly Glu Val Gln Phe Gln Thr Glu Val Glu290 295 300Thr Ile Ser Leu Ala Leu His Arg Asn Leu Leu Arg Leu Arg Gly Phe305 310 315 320Cys Ser Ser Asn Gln Glu Arg Ile Leu Val Tyr Pro Tyr Met Pro Asn325 330 335Gly Ser Val Ala Ser Arg Leu Lys Asp Asn Ile Arg Gly Glu Pro Ala340 345 350Leu Asp Trp Ser Arg Arg Lys Lys Ile Ala Val Gly Thr Ala Arg Gly355 360 365Leu Val Tyr Leu His Glu Gln Cys Asp Pro Lys Ile Ile His Arg Asp370 375 380Val Lys Ala Ala Asn Ile Leu Leu Asp Glu Asp Phe Glu Ala Val Val385 390 395 400Gly Asp Phe Gly Leu Ala Lys Leu Leu Asp His Arg Asp Ser His Val405 410 415Thr Thr Ala Val Arg Gly Thr Val Gly His Ile Ala Pro Glu Tyr Leu420 425 430Ser Thr Gly Gln Ser Ser Glu Lys Thr Asp Val Phe Gly Phe Gly Ile435 440 445Leu Leu Leu Glu Leu Ile Thr Gly Gln Lys Ala Leu Asp Phe Gly Arg450 455 460Ser Ala His Gln Lys Gly Val Met Leu Asp Trp Val Lys Lys Leu His465 470 475 480Gln Glu Gly Lys Leu Lys Gln Leu Ile Asp Lys Asp Leu Asn Asp Lys485 490 495Phe Asp Arg Val Glu Leu Glu Glu Ile Val Gln Val Ala Leu Leu Cys500 505 510Thr Gln Phe Asn Pro Ser His Arg Pro Lys Met Ser Glu Val Met Lys515 520 525Met Leu Glu Gly Asp Gly Leu Ala Glu Arg Trp Glu Ala Thr Gln Asn530 535 540Gly Thr Gly Glu His Gln Pro Pro Pro Leu Pro Pro Gly Met Val Ser545 550 555 560Ser Ser Pro Arg Val Arg Tyr Tyr Ser Asp Tyr Ile Gln Glu Ser Ser565 570 575Leu Val Val Glu Ala Ile Glu Leu Ser Gly Pro Arg580 585361740DNAArabidopsis thalianaCDS(25)..(1737)/note="Arabidopsis thaliana RKS2 cDNA" 36tcaattttgg tagctcttag aaaa atg gct ctg ctt att atc act gcc tta 51Met Ala Leu Leu Ile Ile Thr Ala Leu1 5gtt ttt agt agt tta tgg tca tct gtg tca cca gat gct caa ggg gat 99Val Phe Ser Ser Leu Trp Ser Ser Val Ser Pro Asp Ala Gln Gly Asp10 15 20 25gca tta ttt gcg ttg agg agc tcg tta cgt gca tct cct gaa cag ctt 147Ala Leu Phe Ala Leu Arg Ser Ser Leu Arg Ala Ser Pro Glu Gln Leu30 35 40agt gat tgg aac cag aat caa gtc gat cct tgt act tgg tct caa gtt 195Ser Asp Trp Asn Gln Asn Gln Val Asp Pro Cys Thr Trp Ser Gln Val45 50 55att tgt gat gac aag aaa cat gtt act tct gta acc ttg tct tac atg 243Ile Cys Asp Asp Lys Lys His Val Thr Ser Val Thr Leu Ser Tyr Met60 65 70aac ttc tcc tcg gga aca ctg tct tca gga ata gga atc ttg aca act 291Asn Phe Ser Ser Gly Thr Leu Ser Ser Gly Ile Gly Ile Leu Thr Thr75 80 85ctc aag act ctt aca ttg aag gga aat gga ata atg ggt gga ata cca 339Leu Lys Thr Leu Thr Leu Lys Gly Asn Gly Ile Met Gly Gly Ile Pro90 95 100 105gaa tcc att gga aat ctg tct agc ttg acc agc tta gat ttg gag gat 387Glu Ser Ile Gly Asn Leu Ser Ser Leu Thr Ser Leu Asp Leu Glu Asp110 115 120aat cac tta act gat cgc att cca tcc act ctc ggt aat ctc aag aat 435Asn His Leu Thr Asp Arg Ile Pro Ser Thr Leu Gly Asn Leu Lys Asn125 130 135cta cag ttc ttt ttc aca gca aac aac ttg agc tgt ggt ggc act ttc 483Leu Gln Phe Phe Phe Thr Ala Asn Asn Leu Ser Cys Gly Gly Thr Phe140 145 150ccg caa cct tgt gta acc gag tcc agt cct tca ggt gat tca agc agt 531Pro Gln Pro Cys Val Thr Glu Ser Ser Pro Ser Gly Asp Ser Ser Ser155 160 165aga aaa act gga atc atc gct gga gtt gtt agc gga ata gcg gtt att 579Arg Lys Thr Gly Ile Ile Ala Gly Val Val Ser Gly Ile Ala Val Ile170 175 180 185cta cta gga ttc ttc ttc ttt ttc ttc tgc aag gat aaa cat aaa gga 627Leu Leu Gly Phe Phe Phe Phe Phe Phe Cys Lys Asp Lys His Lys Gly190 195 200tat aaa cga gac gta ttt gtg gat gtt gca gga acg aac ttt aaa aaa 675Tyr Lys Arg Asp Val Phe Val Asp Val Ala Gly Thr Asn Phe Lys Lys205 210 215ggt ttg att tca ggt gaa gtg gac aga agg att gct ttt gga cag ttg 723Gly Leu Ile Ser Gly Glu Val Asp Arg Arg Ile Ala Phe Gly Gln Leu220 225 230aga aga ttt gca tgg aga gag ctt cag ttg gct aca gat gag ttc agt 771Arg Arg Phe Ala Trp Arg Glu Leu Gln Leu Ala Thr Asp Glu Phe Ser235 240 245gaa aag aat gtt ctc gga caa gga ggc ttt ggg aaa gtt tac aaa gga 819Glu Lys Asn Val Leu Gly Gln Gly Gly Phe Gly Lys Val Tyr Lys Gly250 255 260 265ttg ctt tcg gat ggc acc aaa gtc gct gta aaa aga ttg act gat ttt 867Leu Leu Ser Asp Gly Thr Lys Val Ala Val Lys Arg Leu Thr Asp Phe270 275 280gaa cgt cca gga gga gat gaa gct ttc cag aga gaa gtt gag atg ata 915Glu Arg Pro Gly Gly Asp Glu Ala Phe Gln Arg Glu Val Glu Met Ile285 290 295agt gta gct gtt cat agg aat ctg ctt cgc ctt atc ggc ttt tgt aca 963Ser Val Ala Val His Arg Asn Leu Leu Arg Leu Ile Gly Phe Cys Thr300 305 310aca caa act gaa cga ctt ttg gtg tat cct ttc atg cag aat cta agt 1011Thr Gln Thr Glu Arg Leu Leu Val Tyr Pro Phe Met Gln Asn Leu Ser315 320 325gtt gca tat tgc tta aga gag att aaa ccc ggg gat cca gtt ctg gat 1059Val Ala Tyr Cys Leu Arg Glu Ile Lys Pro Gly Asp Pro Val Leu Asp330 335 340 345tgg ttc agg agg aaa cag att gcg tta ggt gca gca cga gga ctc gaa 1107Trp Phe Arg Arg Lys Gln Ile Ala Leu Gly Ala Ala Arg Gly Leu Glu350 355 360tat ctt cat gaa cat tgc aac ccg aag atc ata cac aga gat gtg aaa 1155Tyr Leu His Glu His Cys Asn Pro Lys Ile Ile His Arg Asp Val Lys365 370 375gct gca aat gtg tta cta gat gaa gac ttt gaa gca gtg gtt ggt gat 1203Ala Ala Asn Val Leu Leu Asp Glu Asp Phe Glu Ala Val Val Gly Asp380 385 390ttt ggt tta gcc aag ttg gta gat gtt aga agg act aat gta acc act 1251Phe Gly Leu Ala Lys Leu Val Asp Val Arg Arg Thr Asn Val Thr Thr395 400 405cag gtc cga gga aca atg ggt cat att gca cca gaa tgt ata tcc aca 1299Gln Val Arg Gly Thr Met Gly His Ile Ala Pro Glu Cys Ile Ser Thr410 415 420 425ggg aaa tcg tca gag aaa acc gat gtt ttc ggg tac gga att atg ctt 1347Gly Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Ile Met Leu430 435 440ctg gag ctt gta act gga caa aga gca att gat ttc tcg cgg tta gag 1395Leu Glu Leu Val Thr Gly Gln Arg Ala Ile Asp Phe Ser Arg Leu Glu445 450 455gaa gaa gat gat gtc tta ttg cta gac cat gtg aag aaa ctg gaa aga 1443Glu Glu Asp Asp Val Leu Leu Leu Asp His Val Lys Lys Leu Glu Arg460 465 470gag aag aga tta gaa gac ata gta gat aag aag ctt gat gag gat tat 1491Glu Lys Arg Leu Glu Asp Ile Val Asp Lys Lys Leu Asp Glu Asp Tyr475 480 485ata aag gaa gaa gtt gaa atg atg ata caa gta gct ctg cta tgc aca 1539Ile Lys Glu Glu Val Glu Met Met Ile Gln Val Ala Leu Leu Cys Thr490 495 500 505caa gca gca ccg gaa gaa cga cca gcg atg tcg gaa gta gta aga atg 1587Gln Ala Ala Pro Glu Glu Arg Pro Ala Met Ser Glu Val Val Arg Met510 515 520cta gaa gga gaa ggg ctt gca gag aga tgg gaa gag tgg cag aat ctt 1635Leu Glu Gly Glu Gly Leu Ala Glu Arg Trp Glu Glu Trp Gln Asn Leu525 530 535gaa gtg acg aga caa gaa gag ttt cag agg ttg cag agg aga ttt gat 1683Glu Val Thr Arg Gln Glu Glu Phe Gln Arg Leu Gln Arg Arg Phe Asp540 545 550tgg ggt gaa gat tcc att aat aat caa gat gct att gaa tta tct ggt 1731Trp Gly Glu Asp Ser Ile Asn Asn Gln Asp Ala Ile Glu Leu Ser Gly555 560 565gga aga tag 1740Gly Arg570 37571PRTArabidopsis thaliana 37Met Ala Leu Leu Ile Ile Thr Ala Leu Val Phe Ser Ser Leu Trp Ser1 5 10 15Ser Val Ser Pro Asp Ala Gln Gly Asp Ala Leu Phe Ala Leu Arg Ser20 25 30Ser Leu Arg Ala Ser Pro Glu Gln Leu Ser Asp Trp Asn Gln Asn Gln35 40 45Val Asp Pro Cys Thr Trp Ser Gln Val Ile Cys Asp Asp Lys Lys His50 55 60Val Thr Ser Val Thr Leu Ser Tyr Met Asn Phe Ser Ser Gly Thr Leu65 70 75 80Ser Ser Gly Ile Gly Ile Leu Thr Thr Leu Lys Thr Leu Thr Leu Lys85 90 95Gly Asn Gly Ile Met Gly Gly Ile Pro Glu Ser Ile Gly Asn Leu Ser100 105 110Ser Leu Thr Ser Leu Asp Leu Glu Asp Asn His Leu Thr Asp Arg Ile115 120 125Pro Ser Thr Leu Gly Asn Leu Lys Asn Leu Gln Phe Phe Phe Thr Ala130 135 140Asn Asn Leu Ser Cys Gly Gly Thr Phe Pro Gln Pro Cys Val Thr Glu145 150 155 160Ser Ser Pro Ser Gly Asp Ser Ser Ser Arg Lys Thr Gly Ile Ile Ala165 170 175Gly Val Val Ser Gly Ile Ala Val Ile Leu Leu Gly Phe Phe Phe Phe180 185 190Phe Phe Cys Lys Asp Lys His Lys Gly Tyr Lys Arg Asp Val Phe Val195 200 205Asp Val Ala Gly Thr Asn Phe Lys Lys Gly Leu Ile Ser Gly Glu Val210 215 220Asp Arg Arg Ile Ala Phe Gly Gln Leu Arg Arg Phe Ala Trp Arg Glu225 230 235 240Leu Gln Leu Ala Thr Asp Glu Phe Ser Glu Lys Asn Val Leu Gly Gln245 250 255Gly Gly Phe Gly Lys Val Tyr Lys Gly Leu Leu Ser Asp Gly Thr Lys260 265 270Val Ala Val Lys Arg Leu Thr Asp Phe Glu Arg Pro Gly Gly Asp Glu275 280 285Ala Phe Gln Arg Glu Val Glu Met Ile Ser Val Ala Val His Arg Asn290 295 300Leu Leu Arg Leu Ile Gly Phe Cys Thr Thr Gln Thr Glu Arg Leu Leu305 310 315 320Val Tyr Pro Phe Met Gln Asn Leu Ser Val Ala Tyr Cys Leu Arg Glu325 330 335Ile Lys Pro Gly Asp Pro Val Leu Asp Trp Phe Arg Arg Lys Gln Ile340 345 350Ala Leu Gly Ala Ala Arg Gly Leu Glu Tyr Leu His Glu His Cys Asn355 360 365Pro Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Val Leu Leu Asp370 375 380Glu Asp Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala Lys Leu Val385 390 395 400Asp Val Arg Arg Thr Asn Val Thr Thr Gln Val Arg Gly Thr Met Gly405 410 415His Ile Ala Pro Glu Cys Ile Ser Thr Gly Lys Ser Ser Glu Lys Thr420 425 430Asp Val Phe Gly Tyr Gly Ile Met Leu Leu Glu Leu Val Thr Gly Gln435 440 445Arg Ala Ile Asp Phe Ser Arg Leu Glu Glu Glu Asp Asp Val Leu Leu450 455 460Leu Asp His Val Lys Lys Leu Glu Arg Glu Lys Arg Leu Glu Asp Ile465 470 475 480Val Asp Lys Lys Leu Asp Glu Asp Tyr Ile Lys Glu Glu Val Glu Met485 490 495Met Ile Gln Val Ala Leu Leu Cys Thr Gln Ala Ala Pro Glu Glu Arg500 505 510Pro Ala Met Ser Glu Val Val Arg Met Leu Glu Gly Glu Gly Leu Ala515 520 525Glu Arg Trp Glu Glu Trp Gln Asn Leu Glu Val Thr Arg Gln Glu Glu530 535 540Phe Gln Arg Leu Gln Arg Arg Phe Asp Trp Gly Glu Asp Ser Ile Asn545 550 555 560Asn Gln Asp Ala Ile Glu Leu Ser Gly Gly Arg565 570381830DNAArabidopsis thalianaCDS(91)..(1827)/note="Arabidopsis thaliana RKS3 cDNA" 38aacggtgaaa gtttccatga tcctcttcga ggattcattc aaagaaattg ctttagatgg 60aacaatcaga aattgatctt acaatgtttc atg gcc tta gct ttt gtg gga atc 114Met Ala Leu Ala Phe Val Gly Ile1 5act tcg tca aca act caa cca gat atc gaa gga gga gct ctg ttg cag 162Thr Ser Ser Thr Thr Gln Pro Asp Ile Glu Gly Gly Ala Leu Leu Gln10 15 20ctc aga gat tcg ctt aat gat tcg agc aat cgt cta aaa tgg aca cgc 210Leu Arg Asp Ser Leu Asn Asp Ser Ser Asn Arg Leu Lys Trp Thr Arg25 30 35 40gat ttt gtg agc cct tgc tat agt tgg tct tat gtt acc tgc aga ggc 258Asp Phe Val Ser Pro Cys Tyr Ser Trp Ser Tyr Val Thr Cys Arg Gly45 50 55cag agt gtt gtg gct cta aat ctt gcc tcg agt gga ttc aca gga aca 306Gln Ser Val Val Ala Leu Asn Leu Ala Ser Ser Gly Phe Thr Gly Thr60 65 70ctc tct cca gct att aca aaa ctg aag ttc ttg gtt acc tta gag tta 354Leu Ser Pro Ala Ile Thr Lys Leu Lys Phe Leu Val Thr Leu Glu Leu75 80 85cag aac aat agt tta tct ggt gcc tta cca gat tct ctt ggg aac atg 402Gln Asn Asn Ser Leu Ser Gly Ala Leu Pro Asp Ser Leu Gly Asn Met90 95 100gtt aat cta cag act tta aac cta tca gtg aat agt ttc agc gga tcg 450Val Asn Leu Gln Thr Leu Asn Leu Ser Val Asn Ser Phe Ser Gly Ser105 110 115 120ata cca gcg agc tgg agt cag ctc tcg aat cta aag cac ttg gat ctc 498Ile Pro Ala Ser Trp Ser Gln Leu Ser Asn Leu Lys His Leu Asp Leu125 130 135tca tcc aat aat tta aca gga agc atc cca aca caa ttc ttc tca atc 546Ser Ser Asn Asn Leu Thr Gly Ser Ile Pro Thr Gln Phe Phe Ser Ile140 145 150cca aca ttc gat ttt tca gga act cag ctt ata tgc ggt aaa agt ttg 594Pro Thr Phe Asp Phe Ser Gly Thr Gln Leu Ile Cys Gly Lys Ser Leu155 160 165aat

cag cct tgt tct tca agt tct cgt ctt cca gtc aca tcc tcc aag 642Asn Gln Pro Cys Ser Ser Ser Ser Arg Leu Pro Val Thr Ser Ser Lys170 175 180aaa aag ctg aga gac att act ttg act gca agt tgt gtt gct tct ata 690Lys Lys Leu Arg Asp Ile Thr Leu Thr Ala Ser Cys Val Ala Ser Ile185 190 195 200atc tta ttc ctt gga gca atg gtt atg tat cat cac cat cgc gtc cgc 738Ile Leu Phe Leu Gly Ala Met Val Met Tyr His His His Arg Val Arg205 210 215aga acc aaa tac gac atc ttt ttt gat gta gct ggg gaa gat gac agg 786Arg Thr Lys Tyr Asp Ile Phe Phe Asp Val Ala Gly Glu Asp Asp Arg220 225 230aag att tcc ttt gga caa cta aaa cga ttc tct tta cgt gaa atc cag 834Lys Ile Ser Phe Gly Gln Leu Lys Arg Phe Ser Leu Arg Glu Ile Gln235 240 245ctc gca aca gat agt ttc aac gag agc aat ttg ata gga caa gga gga 882Leu Ala Thr Asp Ser Phe Asn Glu Ser Asn Leu Ile Gly Gln Gly Gly250 255 260ttt ggt aaa gta tac aga ggt ttg ctt cca gac aaa aca aaa gtt gca 930Phe Gly Lys Val Tyr Arg Gly Leu Leu Pro Asp Lys Thr Lys Val Ala265 270 275 280gtg aaa cgc ctt gcg gat tac ttc agt cct gga gga gaa gct gct ttc 978Val Lys Arg Leu Ala Asp Tyr Phe Ser Pro Gly Gly Glu Ala Ala Phe285 290 295caa aga gag att cag ctc ata agc gtt gcg gtt cat aaa aat ctc tta 1026Gln Arg Glu Ile Gln Leu Ile Ser Val Ala Val His Lys Asn Leu Leu300 305 310cgc ctt att ggc ttc tgc aca act tcc tct gag aga atc ctt gtt tat 1074Arg Leu Ile Gly Phe Cys Thr Thr Ser Ser Glu Arg Ile Leu Val Tyr315 320 325cca tac atg gaa aat ctt agt gtt gca tat cga cta aga gat ttg aaa 1122Pro Tyr Met Glu Asn Leu Ser Val Ala Tyr Arg Leu Arg Asp Leu Lys330 335 340gcg gga gag gaa gga tta gac tgg cca aca agg aag cgt gta gct ttt 1170Ala Gly Glu Glu Gly Leu Asp Trp Pro Thr Arg Lys Arg Val Ala Phe345 350 355 360ggt tca gct cac ggt tta gag tat cta cac gaa cat tgt aac ccg aag 1218Gly Ser Ala His Gly Leu Glu Tyr Leu His Glu His Cys Asn Pro Lys365 370 375atc ata cac cgc gat ctc aag gct gca aac ata ctt tta gac aac aat 1266Ile Ile His Arg Asp Leu Lys Ala Ala Asn Ile Leu Leu Asp Asn Asn380 385 390ttt gag cca gtt ctt gga gat ttc ggt tta gct aag ctt gtg gac aca 1314Phe Glu Pro Val Leu Gly Asp Phe Gly Leu Ala Lys Leu Val Asp Thr395 400 405tct ctg act cat gtc aca act caa gtc cga ggc aca atg ggt cac att 1362Ser Leu Thr His Val Thr Thr Gln Val Arg Gly Thr Met Gly His Ile410 415 420gcg cca gag tat ctc tgc aca gga aaa tca tct gaa aaa acc gat gtt 1410Ala Pro Glu Tyr Leu Cys Thr Gly Lys Ser Ser Glu Lys Thr Asp Val425 430 435 440ttt ggt tac ggt ata acg ctt ctt gag ctt gtt act ggt cag cgc gca 1458Phe Gly Tyr Gly Ile Thr Leu Leu Glu Leu Val Thr Gly Gln Arg Ala445 450 455atc gat ttt tca cgc ttg gaa gaa gag gaa aat att ctc ttg ctt gat 1506Ile Asp Phe Ser Arg Leu Glu Glu Glu Glu Asn Ile Leu Leu Leu Asp460 465 470cat ata aag aag ttg ctt aga gaa cag aga ctt aga gac att gtt gat 1554His Ile Lys Lys Leu Leu Arg Glu Gln Arg Leu Arg Asp Ile Val Asp475 480 485agc aat ttg act aca tat gac tcc aaa gaa gtt gaa aca atc gtt caa 1602Ser Asn Leu Thr Thr Tyr Asp Ser Lys Glu Val Glu Thr Ile Val Gln490 495 500gtg gct ctt ctc tgc aca caa ggc tca cca gaa gat aga cca gcg atg 1650Val Ala Leu Leu Cys Thr Gln Gly Ser Pro Glu Asp Arg Pro Ala Met505 510 515 520tct gaa gtg gtc aaa atg ctt caa ggg act ggt ggt ttg gct gag aaa 1698Ser Glu Val Val Lys Met Leu Gln Gly Thr Gly Gly Leu Ala Glu Lys525 530 535tgg act gaa tgg gaa caa ctt gaa gaa gtt agg aac aaa gaa gca ttg 1746Trp Thr Glu Trp Glu Gln Leu Glu Glu Val Arg Asn Lys Glu Ala Leu540 545 550ttg ctt ccg act tta ccg gct act tgg gat gaa gaa gaa acc acc gtt 1794Leu Leu Pro Thr Leu Pro Ala Thr Trp Asp Glu Glu Glu Thr Thr Val555 560 565gat caa gaa tct atc cga tta tcg aca gca aga tga 1830Asp Gln Glu Ser Ile Arg Leu Ser Thr Ala Arg570 575 39579PRTArabidopsis thaliana 39Met Ala Leu Ala Phe Val Gly Ile Thr Ser Ser Thr Thr Gln Pro Asp1 5 10 15Ile Glu Gly Gly Ala Leu Leu Gln Leu Arg Asp Ser Leu Asn Asp Ser20 25 30Ser Asn Arg Leu Lys Trp Thr Arg Asp Phe Val Ser Pro Cys Tyr Ser35 40 45Trp Ser Tyr Val Thr Cys Arg Gly Gln Ser Val Val Ala Leu Asn Leu50 55 60Ala Ser Ser Gly Phe Thr Gly Thr Leu Ser Pro Ala Ile Thr Lys Leu65 70 75 80Lys Phe Leu Val Thr Leu Glu Leu Gln Asn Asn Ser Leu Ser Gly Ala85 90 95Leu Pro Asp Ser Leu Gly Asn Met Val Asn Leu Gln Thr Leu Asn Leu100 105 110Ser Val Asn Ser Phe Ser Gly Ser Ile Pro Ala Ser Trp Ser Gln Leu115 120 125Ser Asn Leu Lys His Leu Asp Leu Ser Ser Asn Asn Leu Thr Gly Ser130 135 140Ile Pro Thr Gln Phe Phe Ser Ile Pro Thr Phe Asp Phe Ser Gly Thr145 150 155 160Gln Leu Ile Cys Gly Lys Ser Leu Asn Gln Pro Cys Ser Ser Ser Ser165 170 175Arg Leu Pro Val Thr Ser Ser Lys Lys Lys Leu Arg Asp Ile Thr Leu180 185 190Thr Ala Ser Cys Val Ala Ser Ile Ile Leu Phe Leu Gly Ala Met Val195 200 205Met Tyr His His His Arg Val Arg Arg Thr Lys Tyr Asp Ile Phe Phe210 215 220Asp Val Ala Gly Glu Asp Asp Arg Lys Ile Ser Phe Gly Gln Leu Lys225 230 235 240Arg Phe Ser Leu Arg Glu Ile Gln Leu Ala Thr Asp Ser Phe Asn Glu245 250 255Ser Asn Leu Ile Gly Gln Gly Gly Phe Gly Lys Val Tyr Arg Gly Leu260 265 270Leu Pro Asp Lys Thr Lys Val Ala Val Lys Arg Leu Ala Asp Tyr Phe275 280 285Ser Pro Gly Gly Glu Ala Ala Phe Gln Arg Glu Ile Gln Leu Ile Ser290 295 300Val Ala Val His Lys Asn Leu Leu Arg Leu Ile Gly Phe Cys Thr Thr305 310 315 320Ser Ser Glu Arg Ile Leu Val Tyr Pro Tyr Met Glu Asn Leu Ser Val325 330 335Ala Tyr Arg Leu Arg Asp Leu Lys Ala Gly Glu Glu Gly Leu Asp Trp340 345 350Pro Thr Arg Lys Arg Val Ala Phe Gly Ser Ala His Gly Leu Glu Tyr355 360 365Leu His Glu His Cys Asn Pro Lys Ile Ile His Arg Asp Leu Lys Ala370 375 380Ala Asn Ile Leu Leu Asp Asn Asn Phe Glu Pro Val Leu Gly Asp Phe385 390 395 400Gly Leu Ala Lys Leu Val Asp Thr Ser Leu Thr His Val Thr Thr Gln405 410 415Val Arg Gly Thr Met Gly His Ile Ala Pro Glu Tyr Leu Cys Thr Gly420 425 430Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Ile Thr Leu Leu435 440 445Glu Leu Val Thr Gly Gln Arg Ala Ile Asp Phe Ser Arg Leu Glu Glu450 455 460Glu Glu Asn Ile Leu Leu Leu Asp His Ile Lys Lys Leu Leu Arg Glu465 470 475 480Gln Arg Leu Arg Asp Ile Val Asp Ser Asn Leu Thr Thr Tyr Asp Ser485 490 495Lys Glu Val Glu Thr Ile Val Gln Val Ala Leu Leu Cys Thr Gln Gly500 505 510Ser Pro Glu Asp Arg Pro Ala Met Ser Glu Val Val Lys Met Leu Gln515 520 525Gly Thr Gly Gly Leu Ala Glu Lys Trp Thr Glu Trp Glu Gln Leu Glu530 535 540Glu Val Arg Asn Lys Glu Ala Leu Leu Leu Pro Thr Leu Pro Ala Thr545 550 555 560Trp Asp Glu Glu Glu Thr Thr Val Asp Gln Glu Ser Ile Arg Leu Ser565 570 575Thr Ala Arg401587DNAArabidopsis thalianaCDS(40)..(1584)/note="Arabidopsis thaliana RKS4 cDNA" 40tcttccttct ccttctggta atctaatcta aagcttttc atg gtg gtg atg aag 54Met Val Val Met Lys1 5ata ttc tct gtt ctg tta cta cta tgt ttc ttc gtt act tgt tct ctc 102Ile Phe Ser Val Leu Leu Leu Leu Cys Phe Phe Val Thr Cys Ser Leu10 15 20tct tct gaa ccc aga aac cct gaa gtc att aat ggt gac aaa ttc ttc 150Ser Ser Glu Pro Arg Asn Pro Glu Val Ile Asn Gly Asp Lys Phe Phe25 30 35atc ttt gtt ttg ttt ttt ccc aat tcc aga gga gct cca agt cag tct 198Ile Phe Val Leu Phe Phe Pro Asn Ser Arg Gly Ala Pro Ser Gln Ser40 45 50ctt tca gga act tta tct ggg tct att gga aat ctc act aat ctt cga 246Leu Ser Gly Thr Leu Ser Gly Ser Ile Gly Asn Leu Thr Asn Leu Arg55 60 65caa gtg tca tta cag aac aat aac atc tcc ggt aaa atc cca ccg gag 294Gln Val Ser Leu Gln Asn Asn Asn Ile Ser Gly Lys Ile Pro Pro Glu70 75 80 85att tgt tct ctt ccc aaa tta cag act ctg gat tta tcc aat aac cgg 342Ile Cys Ser Leu Pro Lys Leu Gln Thr Leu Asp Leu Ser Asn Asn Arg90 95 100ttc tcc ggt gaa atc ccc ggt tct gtt aac cag ctg agt aat ctc caa 390Phe Ser Gly Glu Ile Pro Gly Ser Val Asn Gln Leu Ser Asn Leu Gln105 110 115tat ctt gtt gct ggg aac cct ttg att tgt aaa aac agc cta ccg gag 438Tyr Leu Val Ala Gly Asn Pro Leu Ile Cys Lys Asn Ser Leu Pro Glu120 125 130att tgt tca gga tca atc agt gca agc cct ctt tct gtc tct tta cgt 486Ile Cys Ser Gly Ser Ile Ser Ala Ser Pro Leu Ser Val Ser Leu Arg135 140 145tct tca tca gac aag caa gag gaa ggg tta ctt ggg ttg gga aat cta 534Ser Ser Ser Asp Lys Gln Glu Glu Gly Leu Leu Gly Leu Gly Asn Leu150 155 160 165aga agc ttc aca ttc agg gaa ctt cat gta gct acg gat ggt ttt agt 582Arg Ser Phe Thr Phe Arg Glu Leu His Val Ala Thr Asp Gly Phe Ser170 175 180tcc aag agt att ctt ggt gct ggt ggg ttt ggt aat gtc tac aga gga 630Ser Lys Ser Ile Leu Gly Ala Gly Gly Phe Gly Asn Val Tyr Arg Gly185 190 195aaa ttc ggg gat ggg aca gtg gtt gca gtg aaa cga ttg aaa gat gtg 678Lys Phe Gly Asp Gly Thr Val Val Ala Val Lys Arg Leu Lys Asp Val200 205 210aat gga acc tcc ggg aac tca cag ttt cgt act gag ctt gag atg atc 726Asn Gly Thr Ser Gly Asn Ser Gln Phe Arg Thr Glu Leu Glu Met Ile215 220 225agc tta gct gtt cat agg aat ttg ctt cgg tta atc ggt tat tgt gcg 774Ser Leu Ala Val His Arg Asn Leu Leu Arg Leu Ile Gly Tyr Cys Ala230 235 240 245agt tct agc gaa aga ctt ctt gtt tac cct tac atg tcc aat ggc agc 822Ser Ser Ser Glu Arg Leu Leu Val Tyr Pro Tyr Met Ser Asn Gly Ser250 255 260gtc gcc tct agg ctc aaa gct aag cca gcg ttg gac tgg aac aca agg 870Val Ala Ser Arg Leu Lys Ala Lys Pro Ala Leu Asp Trp Asn Thr Arg265 270 275aag aag ata gcg att gga gct gca aga ggg ttg ttt tat cta cac gag 918Lys Lys Ile Ala Ile Gly Ala Ala Arg Gly Leu Phe Tyr Leu His Glu280 285 290caa tgc gat ccc aag att att cac cga gat gtc aag gca gca aac att 966Gln Cys Asp Pro Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile295 300 305ctc cta gat gag tat ttt gaa gca gtt gtt ggg gat ttt gga cta gca 1014Leu Leu Asp Glu Tyr Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala310 315 320 325aag cta ctc aac cac gag gat tca cat gtc aca acc gcg gtt aga gga 1062Lys Leu Leu Asn His Glu Asp Ser His Val Thr Thr Ala Val Arg Gly330 335 340act gtt ggt cac att gca cct gag tat ctc tcc acc ggt cag tca tct 1110Thr Val Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Gln Ser Ser345 350 355gag aaa acc gat gtc ttt ggg ttc ggt ata ctt ttg cta gag ctc atc 1158Glu Lys Thr Asp Val Phe Gly Phe Gly Ile Leu Leu Leu Glu Leu Ile360 365 370aca gga atg aga gct ctc gag ttt ggc aag tct gtt agc cag aaa gga 1206Thr Gly Met Arg Ala Leu Glu Phe Gly Lys Ser Val Ser Gln Lys Gly375 380 385gct atg cta gaa tgg gtg agg aag cta cac aag gaa atg aaa gta gag 1254Ala Met Leu Glu Trp Val Arg Lys Leu His Lys Glu Met Lys Val Glu390 395 400 405gag cta gta gac cga gaa ctg ggg aca acc tac gat aga ata gaa gtt 1302Glu Leu Val Asp Arg Glu Leu Gly Thr Thr Tyr Asp Arg Ile Glu Val410 415 420gga gag atg cta caa gtg gca ctg ctc tgc act cag ttt ctt cca gct 1350Gly Glu Met Leu Gln Val Ala Leu Leu Cys Thr Gln Phe Leu Pro Ala425 430 435cac aga ccc aaa atg tct gaa gta gtt cag atg ctt gaa gga gat gga 1398His Arg Pro Lys Met Ser Glu Val Val Gln Met Leu Glu Gly Asp Gly440 445 450tta gct gag aga tgg gct gct tca cat gac cat tca cat ttc tac cat 1446Leu Ala Glu Arg Trp Ala Ala Ser His Asp His Ser His Phe Tyr His455 460 465gcc aac atg tct tac agg act att acc tct act gat ggc aac aac caa 1494Ala Asn Met Ser Tyr Arg Thr Ile Thr Ser Thr Asp Gly Asn Asn Gln470 475 480 485acc aaa cat ctg ttt ggc tcc tca gga ttt gaa gat gaa gat gat aat 1542Thr Lys His Leu Phe Gly Ser Ser Gly Phe Glu Asp Glu Asp Asp Asn490 495 500caa gcg tta gat tca ttc gcc atg gaa cta tct ggt cca agg tag 1587Gln Ala Leu Asp Ser Phe Ala Met Glu Leu Ser Gly Pro Arg505 510 515 41515PRTArabidopsis thaliana 41Met Val Val Met Lys Ile Phe Ser Val Leu Leu Leu Leu Cys Phe Phe1 5 10 15Val Thr Cys Ser Leu Ser Ser Glu Pro Arg Asn Pro Glu Val Ile Asn20 25 30Gly Asp Lys Phe Phe Ile Phe Val Leu Phe Phe Pro Asn Ser Arg Gly35 40 45Ala Pro Ser Gln Ser Leu Ser Gly Thr Leu Ser Gly Ser Ile Gly Asn50 55 60Leu Thr Asn Leu Arg Gln Val Ser Leu Gln Asn Asn Asn Ile Ser Gly65 70 75 80Lys Ile Pro Pro Glu Ile Cys Ser Leu Pro Lys Leu Gln Thr Leu Asp85 90 95Leu Ser Asn Asn Arg Phe Ser Gly Glu Ile Pro Gly Ser Val Asn Gln100 105 110Leu Ser Asn Leu Gln Tyr Leu Val Ala Gly Asn Pro Leu Ile Cys Lys115 120 125Asn Ser Leu Pro Glu Ile Cys Ser Gly Ser Ile Ser Ala Ser Pro Leu130 135 140Ser Val Ser Leu Arg Ser Ser Ser Asp Lys Gln Glu Glu Gly Leu Leu145 150 155 160Gly Leu Gly Asn Leu Arg Ser Phe Thr Phe Arg Glu Leu His Val Ala165 170 175Thr Asp Gly Phe Ser Ser Lys Ser Ile Leu Gly Ala Gly Gly Phe Gly180 185 190Asn Val Tyr Arg Gly Lys Phe Gly Asp Gly Thr Val Val Ala Val Lys195 200 205Arg Leu Lys Asp Val Asn Gly Thr Ser Gly Asn Ser Gln Phe Arg Thr210 215 220Glu Leu Glu Met Ile Ser Leu Ala Val His Arg Asn Leu Leu Arg Leu225 230 235 240Ile Gly Tyr Cys Ala Ser Ser Ser Glu Arg Leu Leu Val Tyr Pro Tyr245 250 255Met Ser Asn Gly Ser Val Ala Ser Arg Leu Lys Ala Lys Pro Ala Leu260 265 270Asp Trp Asn Thr Arg Lys Lys Ile Ala Ile Gly Ala Ala Arg Gly Leu275 280 285Phe Tyr Leu His Glu Gln Cys Asp Pro Lys Ile Ile His Arg Asp Val290 295 300Lys Ala Ala Asn Ile Leu Leu Asp Glu Tyr Phe Glu Ala Val Val Gly305 310 315 320Asp Phe Gly Leu Ala Lys Leu Leu Asn His Glu Asp Ser His Val Thr325 330 335Thr Ala Val Arg Gly Thr Val Gly His Ile Ala Pro Glu Tyr Leu Ser340 345 350Thr Gly Gln Ser Ser Glu Lys Thr Asp Val Phe Gly Phe Gly Ile Leu355 360 365Leu Leu Glu Leu Ile Thr Gly Met Arg Ala Leu Glu Phe Gly Lys Ser370 375 380Val Ser Gln Lys Gly Ala Met Leu Glu Trp Val Arg Lys Leu His Lys385 390 395 400Glu Met Lys Val Glu Glu Leu Val Asp Arg Glu Leu Gly Thr Thr Tyr405 410 415Asp Arg Ile Glu Val Gly Glu Met Leu Gln Val Ala Leu Leu Cys Thr420 425 430Gln Phe Leu Pro Ala His Arg Pro Lys Met Ser Glu Val Val Gln Met435 440 445Leu Glu Gly Asp Gly Leu Ala Glu Arg Trp Ala Ala Ser His Asp His450 455 460Ser His Phe Tyr His Ala Asn Met Ser Tyr Arg Thr Ile Thr Ser Thr465 470 475 480Asp Gly Asn Asn Gln Thr Lys His Leu Phe Gly Ser Ser Gly Phe Glu485 490 495Asp Glu Asp Asp Asn Gln Ala Leu Asp Ser Phe Ala Met Glu Leu Ser500 505

510Gly Pro Arg515421629DNAArabidopsis thalianaCDS(28)..(1623)/note="Arabidopsis thaliana RKS5 cDNA" 42ctagagaatt cttatacttt ttctacg atg gag att tct ttg atg aag ttt ctg 54Met Glu Ile Ser Leu Met Lys Phe Leu1 5ttt tta gga atc tgg gtt tat tat tac tct gtt ctt gac tct gtt tct 102Phe Leu Gly Ile Trp Val Tyr Tyr Tyr Ser Val Leu Asp Ser Val Ser10 15 20 25gcc atg gat agt ctt tta tct ccc aag ggt gtt aac tat gaa gtg gct 150Ala Met Asp Ser Leu Leu Ser Pro Lys Gly Val Asn Tyr Glu Val Ala30 35 40gcg tta atg tca gtg aag aac aag atg aaa gat gag aaa gag gtt ttg 198Ala Leu Met Ser Val Lys Asn Lys Met Lys Asp Glu Lys Glu Val Leu45 50 55tct ggt tgg gat att aac tct gtt gat cct tgt act tgg aac atg gtt 246Ser Gly Trp Asp Ile Asn Ser Val Asp Pro Cys Thr Trp Asn Met Val60 65 70ggt tgt tct tct gaa ggt ttt gtg gtt tct ctg tta ctt cag aat aat 294Gly Cys Ser Ser Glu Gly Phe Val Val Ser Leu Leu Leu Gln Asn Asn75 80 85cag tta act ggt ccg att cct tct gag tta ggc caa ctc tct gag ctt 342Gln Leu Thr Gly Pro Ile Pro Ser Glu Leu Gly Gln Leu Ser Glu Leu90 95 100 105gaa acg ctt gat tta tcg ggg aat cgg ttt agt ggt gaa atc cca gct 390Glu Thr Leu Asp Leu Ser Gly Asn Arg Phe Ser Gly Glu Ile Pro Ala110 115 120tct tta ggg ttc tta act cac tta aac tac ttg cgg ctt agc agg aat 438Ser Leu Gly Phe Leu Thr His Leu Asn Tyr Leu Arg Leu Ser Arg Asn125 130 135ctt tta tct ggg caa gtc cct cac ctc gtc gct ggc ctc tca ggt ctt 486Leu Leu Ser Gly Gln Val Pro His Leu Val Ala Gly Leu Ser Gly Leu140 145 150tct ttc ttg gat cta tct ttc aac aat cta agc gga cca act ccg aat 534Ser Phe Leu Asp Leu Ser Phe Asn Asn Leu Ser Gly Pro Thr Pro Asn155 160 165ata tca gca aaa gat tac agg att gta gga aat gca ttt ctt tgt ggt 582Ile Ser Ala Lys Asp Tyr Arg Ile Val Gly Asn Ala Phe Leu Cys Gly170 175 180 185cca gct tcc caa gag ctt tgc tca gat gct aca cct gtg aga aat gtg 630Pro Ala Ser Gln Glu Leu Cys Ser Asp Ala Thr Pro Val Arg Asn Val190 195 200cag caa gac tac gaa ttt gaa atc ggc cat ctg aaa agg ttc agt ttt 678Gln Gln Asp Tyr Glu Phe Glu Ile Gly His Leu Lys Arg Phe Ser Phe205 210 215cgc gaa ata caa acc gca aca agc aat ttt agt cca aag aac att ttg 726Arg Glu Ile Gln Thr Ala Thr Ser Asn Phe Ser Pro Lys Asn Ile Leu220 225 230gga caa gga ggg ttt ggg atg gtt tat aaa ggg tat ctc cca aat gga 774Gly Gln Gly Gly Phe Gly Met Val Tyr Lys Gly Tyr Leu Pro Asn Gly235 240 245act gtg gtg gca gtt aaa aga ttg aaa gat ccg att tat aca gga gaa 822Thr Val Val Ala Val Lys Arg Leu Lys Asp Pro Ile Tyr Thr Gly Glu250 255 260 265gtt cag ttt caa acc gaa gta gag atg att ggc tta gct gtt cac cgt 870Val Gln Phe Gln Thr Glu Val Glu Met Ile Gly Leu Ala Val His Arg270 275 280aac ctt tta cgc ctc ttt gga ttc tgt atg acc ccg gaa gag aga atg 918Asn Leu Leu Arg Leu Phe Gly Phe Cys Met Thr Pro Glu Glu Arg Met285 290 295ctt gtg tat ccg tac atg cca aat gga agc gta gct gat cgt ctg aga 966Leu Val Tyr Pro Tyr Met Pro Asn Gly Ser Val Ala Asp Arg Leu Arg300 305 310gat tgg aat cgg agg ata agc att gca ctc ggc gca gct cga gga ctt 1014Asp Trp Asn Arg Arg Ile Ser Ile Ala Leu Gly Ala Ala Arg Gly Leu315 320 325gtt tac ttg cac gag caa tgc aat cca aag att att cac aga gac gtc 1062Val Tyr Leu His Glu Gln Cys Asn Pro Lys Ile Ile His Arg Asp Val330 335 340 345aaa gct gca aat att cta ctt gat gag agc ttt gaa gca ata gtt ggc 1110Lys Ala Ala Asn Ile Leu Leu Asp Glu Ser Phe Glu Ala Ile Val Gly350 355 360gat ttt ggt cta gca aag ctt tta gac cag aga gat tca cat gtc act 1158Asp Phe Gly Leu Ala Lys Leu Leu Asp Gln Arg Asp Ser His Val Thr365 370 375acc gca gtc cga gga acc att gga cac atc gct ccc gag tac ctt tcc 1206Thr Ala Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu Ser380 385 390act gga cag tcc tca gag aaa acc gat gtt ttc gga ttc gga gta cta 1254Thr Gly Gln Ser Ser Glu Lys Thr Asp Val Phe Gly Phe Gly Val Leu395 400 405atc ctt gaa ctc ata aca ggt cat aag atg att gat caa ggc aat ggt 1302Ile Leu Glu Leu Ile Thr Gly His Lys Met Ile Asp Gln Gly Asn Gly410 415 420 425caa gtt cga aaa gga atg ata ttg agc tgg gta agg aca ttg aaa gca 1350Gln Val Arg Lys Gly Met Ile Leu Ser Trp Val Arg Thr Leu Lys Ala430 435 440gag aag aga ttt gca gag atg gtg gac aga gat ttg aag gga gag ttt 1398Glu Lys Arg Phe Ala Glu Met Val Asp Arg Asp Leu Lys Gly Glu Phe445 450 455gat gat ttg gtg ttg gag gaa gta gtg gaa ttg gct ttg ctt tgt aca 1446Asp Asp Leu Val Leu Glu Glu Val Val Glu Leu Ala Leu Leu Cys Thr460 465 470cag cca cat ccg aat cta aga ccg agg atg tct caa gtg ttg aag gta 1494Gln Pro His Pro Asn Leu Arg Pro Arg Met Ser Gln Val Leu Lys Val475 480 485cta gaa ggt tta gtg gaa cag tgt gaa gga ggg tat gaa gct aga gct 1542Leu Glu Gly Leu Val Glu Gln Cys Glu Gly Gly Tyr Glu Ala Arg Ala490 495 500 505cca agt gtc tct agg aac tac agt aat ggt cat gaa gag cag tcc ttt 1590Pro Ser Val Ser Arg Asn Tyr Ser Asn Gly His Glu Glu Gln Ser Phe510 515 520att att gaa gcc att gag ctc tct gga cca cga tgatag 1629Ile Ile Glu Ala Ile Glu Leu Ser Gly Pro Arg525 530 43532PRTArabidopsis thaliana 43Met Glu Ile Ser Leu Met Lys Phe Leu Phe Leu Gly Ile Trp Val Tyr1 5 10 15Tyr Tyr Ser Val Leu Asp Ser Val Ser Ala Met Asp Ser Leu Leu Ser20 25 30Pro Lys Gly Val Asn Tyr Glu Val Ala Ala Leu Met Ser Val Lys Asn35 40 45Lys Met Lys Asp Glu Lys Glu Val Leu Ser Gly Trp Asp Ile Asn Ser50 55 60Val Asp Pro Cys Thr Trp Asn Met Val Gly Cys Ser Ser Glu Gly Phe65 70 75 80Val Val Ser Leu Leu Leu Gln Asn Asn Gln Leu Thr Gly Pro Ile Pro85 90 95Ser Glu Leu Gly Gln Leu Ser Glu Leu Glu Thr Leu Asp Leu Ser Gly100 105 110Asn Arg Phe Ser Gly Glu Ile Pro Ala Ser Leu Gly Phe Leu Thr His115 120 125Leu Asn Tyr Leu Arg Leu Ser Arg Asn Leu Leu Ser Gly Gln Val Pro130 135 140His Leu Val Ala Gly Leu Ser Gly Leu Ser Phe Leu Asp Leu Ser Phe145 150 155 160Asn Asn Leu Ser Gly Pro Thr Pro Asn Ile Ser Ala Lys Asp Tyr Arg165 170 175Ile Val Gly Asn Ala Phe Leu Cys Gly Pro Ala Ser Gln Glu Leu Cys180 185 190Ser Asp Ala Thr Pro Val Arg Asn Val Gln Gln Asp Tyr Glu Phe Glu195 200 205Ile Gly His Leu Lys Arg Phe Ser Phe Arg Glu Ile Gln Thr Ala Thr210 215 220Ser Asn Phe Ser Pro Lys Asn Ile Leu Gly Gln Gly Gly Phe Gly Met225 230 235 240Val Tyr Lys Gly Tyr Leu Pro Asn Gly Thr Val Val Ala Val Lys Arg245 250 255Leu Lys Asp Pro Ile Tyr Thr Gly Glu Val Gln Phe Gln Thr Glu Val260 265 270Glu Met Ile Gly Leu Ala Val His Arg Asn Leu Leu Arg Leu Phe Gly275 280 285Phe Cys Met Thr Pro Glu Glu Arg Met Leu Val Tyr Pro Tyr Met Pro290 295 300Asn Gly Ser Val Ala Asp Arg Leu Arg Asp Trp Asn Arg Arg Ile Ser305 310 315 320Ile Ala Leu Gly Ala Ala Arg Gly Leu Val Tyr Leu His Glu Gln Cys325 330 335Asn Pro Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile Leu Leu340 345 350Asp Glu Ser Phe Glu Ala Ile Val Gly Asp Phe Gly Leu Ala Lys Leu355 360 365Leu Asp Gln Arg Asp Ser His Val Thr Thr Ala Val Arg Gly Thr Ile370 375 380Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Gln Ser Ser Glu Lys385 390 395 400Thr Asp Val Phe Gly Phe Gly Val Leu Ile Leu Glu Leu Ile Thr Gly405 410 415His Lys Met Ile Asp Gln Gly Asn Gly Gln Val Arg Lys Gly Met Ile420 425 430Leu Ser Trp Val Arg Thr Leu Lys Ala Glu Lys Arg Phe Ala Glu Met435 440 445Val Asp Arg Asp Leu Lys Gly Glu Phe Asp Asp Leu Val Leu Glu Glu450 455 460Val Val Glu Leu Ala Leu Leu Cys Thr Gln Pro His Pro Asn Leu Arg465 470 475 480Pro Arg Met Ser Gln Val Leu Lys Val Leu Glu Gly Leu Val Glu Gln485 490 495Cys Glu Gly Gly Tyr Glu Ala Arg Ala Pro Ser Val Ser Arg Asn Tyr500 505 510Ser Asn Gly His Glu Glu Gln Ser Phe Ile Ile Glu Ala Ile Glu Leu515 520 525Ser Gly Pro Arg530441920DNAArabidopsis thalianaCDS(61)..(1899)/note="Arabidopsis thaliana RKS6 cDNA" 44attgtttcct tcttttggga ttttctcctt ggatggaacc agctcaatta atgagatgag 60atg aga atg ttc agc ttg cag aag atg gct atg gct ttt act ctc ttg 108Met Arg Met Phe Ser Leu Gln Lys Met Ala Met Ala Phe Thr Leu Leu1 5 10 15ttt ttt gcc tgt tta tgc tca ttt gtg tct cca gat gct caa ggg gat 156Phe Phe Ala Cys Leu Cys Ser Phe Val Ser Pro Asp Ala Gln Gly Asp20 25 30gca ctg ttt gcg ttg agg atc tcc tta cgt gca tta ccg aat cag cta 204Ala Leu Phe Ala Leu Arg Ile Ser Leu Arg Ala Leu Pro Asn Gln Leu35 40 45agt gac tgg aat cag aac caa gtt aat cct tgc act tgg tcc caa gtt 252Ser Asp Trp Asn Gln Asn Gln Val Asn Pro Cys Thr Trp Ser Gln Val50 55 60att tgt gat gac aaa aac ttt gtc act tct ctt aca ttg tca gat atg 300Ile Cys Asp Asp Lys Asn Phe Val Thr Ser Leu Thr Leu Ser Asp Met65 70 75 80aac ttc tcg gga acc ttg tct tca aga gta gga atc cta gaa aat ctc 348Asn Phe Ser Gly Thr Leu Ser Ser Arg Val Gly Ile Leu Glu Asn Leu85 90 95aag act ctt act tta aag gga aat gga att acg ggt gaa ata cca gaa 396Lys Thr Leu Thr Leu Lys Gly Asn Gly Ile Thr Gly Glu Ile Pro Glu100 105 110gac ttt gga aat ctg act agc ttg act agt ttg gat ttg gag gac aat 444Asp Phe Gly Asn Leu Thr Ser Leu Thr Ser Leu Asp Leu Glu Asp Asn115 120 125cag cta act ggt cgt ata cca tcc act atc ggt aat ctc aag aaa ctt 492Gln Leu Thr Gly Arg Ile Pro Ser Thr Ile Gly Asn Leu Lys Lys Leu130 135 140cag ttc ttg acc ttg agt agg aac aaa ctt aat ggg act att ccg gag 540Gln Phe Leu Thr Leu Ser Arg Asn Lys Leu Asn Gly Thr Ile Pro Glu145 150 155 160tca ctc act ggt ctt cca aac ctg tta aac ctg ctg ctt gat tcc aat 588Ser Leu Thr Gly Leu Pro Asn Leu Leu Asn Leu Leu Leu Asp Ser Asn165 170 175agt ctc agt ggt cag att cct caa agt ctg ttt gag atc cca aaa tat 636Ser Leu Ser Gly Gln Ile Pro Gln Ser Leu Phe Glu Ile Pro Lys Tyr180 185 190aat ttc acg tca aac aac ttg aat tgt ggc ggt cgt caa cct cac cct 684Asn Phe Thr Ser Asn Asn Leu Asn Cys Gly Gly Arg Gln Pro His Pro195 200 205tgt gta tcc gcg gtt gcc cat tca ggt gat tca agc aag cct aaa act 732Cys Val Ser Ala Val Ala His Ser Gly Asp Ser Ser Lys Pro Lys Thr210 215 220ggc att att gct gga gtt gtt gct gga gtt aca gtt gtt ctc ttt gga 780Gly Ile Ile Ala Gly Val Val Ala Gly Val Thr Val Val Leu Phe Gly225 230 235 240atc ttg ttg ttt ctg ttc tgc aag gat agg cat aaa gga tat aga cgt 828Ile Leu Leu Phe Leu Phe Cys Lys Asp Arg His Lys Gly Tyr Arg Arg245 250 255gat gtg ttt gtg gat gtt gca ggt gaa gtg gac agg aga att gca ttt 876Asp Val Phe Val Asp Val Ala Gly Glu Val Asp Arg Arg Ile Ala Phe260 265 270gga cag ttg aaa agg ttt gca tgg aga gag ctc cag tta gcg aca gat 924Gly Gln Leu Lys Arg Phe Ala Trp Arg Glu Leu Gln Leu Ala Thr Asp275 280 285aac ttc agc gaa aag aat gta ctt ggt caa gga ggc ttt ggg aaa gtt 972Asn Phe Ser Glu Lys Asn Val Leu Gly Gln Gly Gly Phe Gly Lys Val290 295 300tac aaa gga gtg ctt ccg gat aca ccc aaa gtt gct gtg aag aga ttg 1020Tyr Lys Gly Val Leu Pro Asp Thr Pro Lys Val Ala Val Lys Arg Leu305 310 315 320acg gat ttc gaa agt cct ggt gga gat gct gct ttc caa agg gaa gta 1068Thr Asp Phe Glu Ser Pro Gly Gly Asp Ala Ala Phe Gln Arg Glu Val325 330 335gag atg ata agt gta gct gtt cat agg aat cta ctc cgt ctt atc ggg 1116Glu Met Ile Ser Val Ala Val His Arg Asn Leu Leu Arg Leu Ile Gly340 345 350ttc tgc acc aca caa aca gaa cgc ctt ttg gtt tat ccc ttc atg cag 1164Phe Cys Thr Thr Gln Thr Glu Arg Leu Leu Val Tyr Pro Phe Met Gln355 360 365aat cta agt ctt gca cat cgt ctg aga gag atc aaa gca ggc gac ccg 1212Asn Leu Ser Leu Ala His Arg Leu Arg Glu Ile Lys Ala Gly Asp Pro370 375 380gtt cta gat tgg gag acg agg aaa cgg att gcc tta gga gca gcg cgt 1260Val Leu Asp Trp Glu Thr Arg Lys Arg Ile Ala Leu Gly Ala Ala Arg385 390 395 400ggt ttt gag tat ctt cat gaa cat tgc aat ccg aag atc ata cat cgt 1308Gly Phe Glu Tyr Leu His Glu His Cys Asn Pro Lys Ile Ile His Arg405 410 415gat gtg aaa gca gct aat gtg tta cta gat gaa gat ttt gaa gca gtg 1356Asp Val Lys Ala Ala Asn Val Leu Leu Asp Glu Asp Phe Glu Ala Val420 425 430gtt ggt gat ttt ggt tta gcc aag cta gta gat gtt aga agg act aat 1404Val Gly Asp Phe Gly Leu Ala Lys Leu Val Asp Val Arg Arg Thr Asn435 440 445gtg act act caa gtt cga gga aca atg ggt cac att gca cca gaa tat 1452Val Thr Thr Gln Val Arg Gly Thr Met Gly His Ile Ala Pro Glu Tyr450 455 460tta tca aca ggg aaa tca tca gag aga acc gat gtt ttc ggg tat gga 1500Leu Ser Thr Gly Lys Ser Ser Glu Arg Thr Asp Val Phe Gly Tyr Gly465 470 475 480att atg ctt ctt gag ctt gtt aca gga caa cgc gca ata gac ttt tca 1548Ile Met Leu Leu Glu Leu Val Thr Gly Gln Arg Ala Ile Asp Phe Ser485 490 495cgt ttg gag gaa gaa gat gat gtc ttg tta ctt gac cac gtg aag aaa 1596Arg Leu Glu Glu Glu Asp Asp Val Leu Leu Leu Asp His Val Lys Lys500 505 510ctg gaa aga gag aag aga tta gga gca atc gta gat aag aat ttg gat 1644Leu Glu Arg Glu Lys Arg Leu Gly Ala Ile Val Asp Lys Asn Leu Asp515 520 525gga gag tat ata aaa gaa gaa gta gag atg atg ata caa gtg gct ttg 1692Gly Glu Tyr Ile Lys Glu Glu Val Glu Met Met Ile Gln Val Ala Leu530 535 540ctt tgt aca caa ggt tca cca gaa gac cga cca gtg atg tct gaa gtt 1740Leu Cys Thr Gln Gly Ser Pro Glu Asp Arg Pro Val Met Ser Glu Val545 550 555 560gtg agg atg tta gaa gga gaa ggg ctt gcg gag aga tgg gaa gag tgg 1788Val Arg Met Leu Glu Gly Glu Gly Leu Ala Glu Arg Trp Glu Glu Trp565 570 575caa aac gtg gaa gtc acg aga cgt cat gag ttt gaa cgg ttg cag agg 1836Gln Asn Val Glu Val Thr Arg Arg His Glu Phe Glu Arg Leu Gln Arg580 585 590aga ttt gat tgg ggt gaa gat tct atg cat aac caa gat gcc att gaa 1884Arg Phe Asp Trp Gly Glu Asp Ser Met His Asn Gln Asp Ala Ile Glu595 600 605tta tct ggt gga aga tgaccaaaaa catcaaacct t 1920Leu Ser Gly Gly Arg610 45613PRTArabidopsis thaliana 45Met Arg Met Phe Ser Leu Gln Lys Met Ala Met Ala Phe Thr Leu Leu1 5 10 15Phe Phe Ala Cys Leu Cys Ser Phe Val Ser Pro Asp Ala Gln Gly Asp20 25 30Ala Leu Phe Ala Leu Arg Ile Ser Leu Arg Ala Leu Pro Asn Gln Leu35 40 45Ser Asp Trp Asn Gln Asn Gln Val Asn Pro Cys Thr Trp Ser Gln Val50 55 60Ile Cys Asp Asp Lys Asn Phe Val Thr Ser Leu Thr Leu Ser Asp Met65 70 75 80Asn Phe Ser Gly Thr Leu Ser Ser Arg Val Gly Ile Leu Glu Asn Leu85 90 95Lys Thr Leu Thr Leu Lys Gly Asn Gly Ile Thr Gly Glu Ile Pro Glu100 105 110Asp Phe Gly Asn Leu Thr Ser Leu Thr Ser Leu Asp Leu Glu Asp Asn115 120 125Gln Leu Thr Gly Arg Ile Pro Ser Thr Ile Gly Asn Leu Lys Lys Leu130 135 140Gln Phe Leu Thr Leu Ser Arg Asn Lys Leu Asn Gly Thr Ile Pro Glu145 150 155 160Ser

Leu Thr Gly Leu Pro Asn Leu Leu Asn Leu Leu Leu Asp Ser Asn165 170 175Ser Leu Ser Gly Gln Ile Pro Gln Ser Leu Phe Glu Ile Pro Lys Tyr180 185 190Asn Phe Thr Ser Asn Asn Leu Asn Cys Gly Gly Arg Gln Pro His Pro195 200 205Cys Val Ser Ala Val Ala His Ser Gly Asp Ser Ser Lys Pro Lys Thr210 215 220Gly Ile Ile Ala Gly Val Val Ala Gly Val Thr Val Val Leu Phe Gly225 230 235 240Ile Leu Leu Phe Leu Phe Cys Lys Asp Arg His Lys Gly Tyr Arg Arg245 250 255Asp Val Phe Val Asp Val Ala Gly Glu Val Asp Arg Arg Ile Ala Phe260 265 270Gly Gln Leu Lys Arg Phe Ala Trp Arg Glu Leu Gln Leu Ala Thr Asp275 280 285Asn Phe Ser Glu Lys Asn Val Leu Gly Gln Gly Gly Phe Gly Lys Val290 295 300Tyr Lys Gly Val Leu Pro Asp Thr Pro Lys Val Ala Val Lys Arg Leu305 310 315 320Thr Asp Phe Glu Ser Pro Gly Gly Asp Ala Ala Phe Gln Arg Glu Val325 330 335Glu Met Ile Ser Val Ala Val His Arg Asn Leu Leu Arg Leu Ile Gly340 345 350Phe Cys Thr Thr Gln Thr Glu Arg Leu Leu Val Tyr Pro Phe Met Gln355 360 365Asn Leu Ser Leu Ala His Arg Leu Arg Glu Ile Lys Ala Gly Asp Pro370 375 380Val Leu Asp Trp Glu Thr Arg Lys Arg Ile Ala Leu Gly Ala Ala Arg385 390 395 400Gly Phe Glu Tyr Leu His Glu His Cys Asn Pro Lys Ile Ile His Arg405 410 415Asp Val Lys Ala Ala Asn Val Leu Leu Asp Glu Asp Phe Glu Ala Val420 425 430Val Gly Asp Phe Gly Leu Ala Lys Leu Val Asp Val Arg Arg Thr Asn435 440 445Val Thr Thr Gln Val Arg Gly Thr Met Gly His Ile Ala Pro Glu Tyr450 455 460Leu Ser Thr Gly Lys Ser Ser Glu Arg Thr Asp Val Phe Gly Tyr Gly465 470 475 480Ile Met Leu Leu Glu Leu Val Thr Gly Gln Arg Ala Ile Asp Phe Ser485 490 495Arg Leu Glu Glu Glu Asp Asp Val Leu Leu Leu Asp His Val Lys Lys500 505 510Leu Glu Arg Glu Lys Arg Leu Gly Ala Ile Val Asp Lys Asn Leu Asp515 520 525Gly Glu Tyr Ile Lys Glu Glu Val Glu Met Met Ile Gln Val Ala Leu530 535 540Leu Cys Thr Gln Gly Ser Pro Glu Asp Arg Pro Val Met Ser Glu Val545 550 555 560Val Arg Met Leu Glu Gly Glu Gly Leu Ala Glu Arg Trp Glu Glu Trp565 570 575Gln Asn Val Glu Val Thr Arg Arg His Glu Phe Glu Arg Leu Gln Arg580 585 590Arg Phe Asp Trp Gly Glu Asp Ser Met His Asn Gln Asp Ala Ile Glu595 600 605Leu Ser Gly Gly Arg610461949DNAArabidopsis thalianaCDS(61)..(1944)/note="Arabidopsis thaliana RKS8 cDNA" 46gttttttttt ttttaccctc ttggaggatc tgggaggaga aatttgcttt tttttggtaa 60atg ggg aga aaa aag ttt gaa gct ttt ggt ttt gtc tgc tta atc tca 108Met Gly Arg Lys Lys Phe Glu Ala Phe Gly Phe Val Cys Leu Ile Ser1 5 10 15ctg ctt ctt ctg ttt aat tcg tta tgg ctt gcc tct tct aac atg gaa 156Leu Leu Leu Leu Phe Asn Ser Leu Trp Leu Ala Ser Ser Asn Met Glu20 25 30ggt gat gca ctg cac agt ttg aga gct aat cta gtt gat cca aat aat 204Gly Asp Ala Leu His Ser Leu Arg Ala Asn Leu Val Asp Pro Asn Asn35 40 45gtc ttg caa agc tgg gat cct acg ctt gtt aat ccg tgt act tgg ttt 252Val Leu Gln Ser Trp Asp Pro Thr Leu Val Asn Pro Cys Thr Trp Phe50 55 60cac gta acg tgt aac aac gag aac agt gtt ata aga gtc gat ctt ggg 300His Val Thr Cys Asn Asn Glu Asn Ser Val Ile Arg Val Asp Leu Gly65 70 75 80aat gca gac ttg tct ggt cag ttg gtt cct cag cta ggt cag ctc aag 348Asn Ala Asp Leu Ser Gly Gln Leu Val Pro Gln Leu Gly Gln Leu Lys85 90 95aac ttg cag tac ttg gag ctt tat agt aat aac ata acc ggg ccg gtt 396Asn Leu Gln Tyr Leu Glu Leu Tyr Ser Asn Asn Ile Thr Gly Pro Val100 105 110cca agc gat ctt ggg aat ctg aca aac tta gtg agc ttg gat ctt tac 444Pro Ser Asp Leu Gly Asn Leu Thr Asn Leu Val Ser Leu Asp Leu Tyr115 120 125ttg aac agc ttc act ggt cca att cca gat tct cta gga aag cta ttc 492Leu Asn Ser Phe Thr Gly Pro Ile Pro Asp Ser Leu Gly Lys Leu Phe130 135 140aag ctt cgc ttt ctt cgg ctc aac aat aac agt ctc acc gga cca att 540Lys Leu Arg Phe Leu Arg Leu Asn Asn Asn Ser Leu Thr Gly Pro Ile145 150 155 160ccc atg tca ttg act aat atc atg acc ctt caa gtt ttg gat ctg tcg 588Pro Met Ser Leu Thr Asn Ile Met Thr Leu Gln Val Leu Asp Leu Ser165 170 175aac aac cga tta tcc gga tct gtt cct gat aat ggt tcc ttc tcg ctc 636Asn Asn Arg Leu Ser Gly Ser Val Pro Asp Asn Gly Ser Phe Ser Leu180 185 190ttc act ccc atc agt ttt gct aac aac ttg gat cta tgc ggc cca gtt 684Phe Thr Pro Ile Ser Phe Ala Asn Asn Leu Asp Leu Cys Gly Pro Val195 200 205act agc cgt cct tgt cct gga tct ccc ccg ttt tct cct cca cca cct 732Thr Ser Arg Pro Cys Pro Gly Ser Pro Pro Phe Ser Pro Pro Pro Pro210 215 220ttt ata cca cct ccc ata gtt cct aca cca ggt ggg tat agt gct act 780Phe Ile Pro Pro Pro Ile Val Pro Thr Pro Gly Gly Tyr Ser Ala Thr225 230 235 240gga gcc att gcg gga gga gtt gct gct ggt gct gct tta cta ttt gct 828Gly Ala Ile Ala Gly Gly Val Ala Ala Gly Ala Ala Leu Leu Phe Ala245 250 255gcc cct gct tta gct ttt gct tgg tgg cgt aga aga aaa cct caa gaa 876Ala Pro Ala Leu Ala Phe Ala Trp Trp Arg Arg Arg Lys Pro Gln Glu260 265 270ttc ttc ttt gat gtt cct gcc gaa gag gac cct gag gtt cac ttg ggg 924Phe Phe Phe Asp Val Pro Ala Glu Glu Asp Pro Glu Val His Leu Gly275 280 285cag ctt aag cgg ttc tct cta cgg gaa ctt caa gta gca act gat agc 972Gln Leu Lys Arg Phe Ser Leu Arg Glu Leu Gln Val Ala Thr Asp Ser290 295 300ttc agc aac aag aac att ttg ggc cga ggt ggg ttc gga aaa gtc tac 1020Phe Ser Asn Lys Asn Ile Leu Gly Arg Gly Gly Phe Gly Lys Val Tyr305 310 315 320aaa ggc cgt ctt gct gat gga aca ctt gtt gca gtc aaa cgg ctt aaa 1068Lys Gly Arg Leu Ala Asp Gly Thr Leu Val Ala Val Lys Arg Leu Lys325 330 335gaa gag cga acc cca ggt ggc gag ctc cag ttt cag aca gaa gtg gag 1116Glu Glu Arg Thr Pro Gly Gly Glu Leu Gln Phe Gln Thr Glu Val Glu340 345 350atg ata agc atg gcc gtt cac aga aat ctc ctc agg cta cgc ggt ttc 1164Met Ile Ser Met Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly Phe355 360 365tgt atg acc cct acc gag aga ttg ctt gtt tat cct tac atg gct aat 1212Cys Met Thr Pro Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn370 375 380gga agt gtc gct tcc tgt ttg aga gaa cgt cca cca tca cag ttg cct 1260Gly Ser Val Ala Ser Cys Leu Arg Glu Arg Pro Pro Ser Gln Leu Pro385 390 395 400cta gcc tgg tca ata aga cag caa atc gcg cta gga tca gcg agg ggt 1308Leu Ala Trp Ser Ile Arg Gln Gln Ile Ala Leu Gly Ser Ala Arg Gly405 410 415ttg tct tat ctt cat gat cat tgc gac ccc aaa att att cac cgt gat 1356Leu Ser Tyr Leu His Asp His Cys Asp Pro Lys Ile Ile His Arg Asp420 425 430gtg aaa gct gct aat att ctg ttg gac gag gaa ttt gag gcg gtg gta 1404Val Lys Ala Ala Asn Ile Leu Leu Asp Glu Glu Phe Glu Ala Val Val435 440 445ggt gat ttc ggg tta gct aga ctt atg gac tat aaa gat act cat gtc 1452Gly Asp Phe Gly Leu Ala Arg Leu Met Asp Tyr Lys Asp Thr His Val450 455 460aca acg gct gtg cgt ggg act att gga cac att gct cct gag tat ctc 1500Thr Thr Ala Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu465 470 475 480tca act gga aaa tct tca gag aaa act gat gtt ttt ggc tac ggg atc 1548Ser Thr Gly Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Ile485 490 495atg ctt ttg gaa ctg att aca ggt cag aga gct ttt gat ctt gca aga 1596Met Leu Leu Glu Leu Ile Thr Gly Gln Arg Ala Phe Asp Leu Ala Arg500 505 510ctg gcg aat gac gat gac gtt atg ctc cta gat tgg gtg aaa ggg ctt 1644Leu Ala Asn Asp Asp Asp Val Met Leu Leu Asp Trp Val Lys Gly Leu515 520 525ttg aag gag aag aag ctg gag atg ctt gtg gat cct gac ctg caa agc 1692Leu Lys Glu Lys Lys Leu Glu Met Leu Val Asp Pro Asp Leu Gln Ser530 535 540aat tac aca gaa gca gaa gta gaa cag ctc ata caa gtg gct ctt ctc 1740Asn Tyr Thr Glu Ala Glu Val Glu Gln Leu Ile Gln Val Ala Leu Leu545 550 555 560tgc aca cag agc tca cct atg gaa cga cct aag atg tct gag gtt gtt 1788Cys Thr Gln Ser Ser Pro Met Glu Arg Pro Lys Met Ser Glu Val Val565 570 575cga atg ctt gaa ggt gac ggt tta gcg gag aaa tgg gac gag tgg cag 1836Arg Met Leu Glu Gly Asp Gly Leu Ala Glu Lys Trp Asp Glu Trp Gln580 585 590aaa gtg gaa gtt ctc agg caa gaa gtg gag ctc tct tct cac ccc acc 1884Lys Val Glu Val Leu Arg Gln Glu Val Glu Leu Ser Ser His Pro Thr595 600 605tct gac tgg atc ctt gat tcg act gat aat ctt cat gct atg gag ttg 1932Ser Asp Trp Ile Leu Asp Ser Thr Asp Asn Leu His Ala Met Glu Leu610 615 620tct ggt cca aga taaac 1949Ser Gly Pro Arg625 47628PRTArabidopsis thaliana 47Met Gly Arg Lys Lys Phe Glu Ala Phe Gly Phe Val Cys Leu Ile Ser1 5 10 15Leu Leu Leu Leu Phe Asn Ser Leu Trp Leu Ala Ser Ser Asn Met Glu20 25 30Gly Asp Ala Leu His Ser Leu Arg Ala Asn Leu Val Asp Pro Asn Asn35 40 45Val Leu Gln Ser Trp Asp Pro Thr Leu Val Asn Pro Cys Thr Trp Phe50 55 60His Val Thr Cys Asn Asn Glu Asn Ser Val Ile Arg Val Asp Leu Gly65 70 75 80Asn Ala Asp Leu Ser Gly Gln Leu Val Pro Gln Leu Gly Gln Leu Lys85 90 95Asn Leu Gln Tyr Leu Glu Leu Tyr Ser Asn Asn Ile Thr Gly Pro Val100 105 110Pro Ser Asp Leu Gly Asn Leu Thr Asn Leu Val Ser Leu Asp Leu Tyr115 120 125Leu Asn Ser Phe Thr Gly Pro Ile Pro Asp Ser Leu Gly Lys Leu Phe130 135 140Lys Leu Arg Phe Leu Arg Leu Asn Asn Asn Ser Leu Thr Gly Pro Ile145 150 155 160Pro Met Ser Leu Thr Asn Ile Met Thr Leu Gln Val Leu Asp Leu Ser165 170 175Asn Asn Arg Leu Ser Gly Ser Val Pro Asp Asn Gly Ser Phe Ser Leu180 185 190Phe Thr Pro Ile Ser Phe Ala Asn Asn Leu Asp Leu Cys Gly Pro Val195 200 205Thr Ser Arg Pro Cys Pro Gly Ser Pro Pro Phe Ser Pro Pro Pro Pro210 215 220Phe Ile Pro Pro Pro Ile Val Pro Thr Pro Gly Gly Tyr Ser Ala Thr225 230 235 240Gly Ala Ile Ala Gly Gly Val Ala Ala Gly Ala Ala Leu Leu Phe Ala245 250 255Ala Pro Ala Leu Ala Phe Ala Trp Trp Arg Arg Arg Lys Pro Gln Glu260 265 270Phe Phe Phe Asp Val Pro Ala Glu Glu Asp Pro Glu Val His Leu Gly275 280 285Gln Leu Lys Arg Phe Ser Leu Arg Glu Leu Gln Val Ala Thr Asp Ser290 295 300Phe Ser Asn Lys Asn Ile Leu Gly Arg Gly Gly Phe Gly Lys Val Tyr305 310 315 320Lys Gly Arg Leu Ala Asp Gly Thr Leu Val Ala Val Lys Arg Leu Lys325 330 335Glu Glu Arg Thr Pro Gly Gly Glu Leu Gln Phe Gln Thr Glu Val Glu340 345 350Met Ile Ser Met Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly Phe355 360 365Cys Met Thr Pro Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn370 375 380Gly Ser Val Ala Ser Cys Leu Arg Glu Arg Pro Pro Ser Gln Leu Pro385 390 395 400Leu Ala Trp Ser Ile Arg Gln Gln Ile Ala Leu Gly Ser Ala Arg Gly405 410 415Leu Ser Tyr Leu His Asp His Cys Asp Pro Lys Ile Ile His Arg Asp420 425 430Val Lys Ala Ala Asn Ile Leu Leu Asp Glu Glu Phe Glu Ala Val Val435 440 445Gly Asp Phe Gly Leu Ala Arg Leu Met Asp Tyr Lys Asp Thr His Val450 455 460Thr Thr Ala Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu465 470 475 480Ser Thr Gly Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Ile485 490 495Met Leu Leu Glu Leu Ile Thr Gly Gln Arg Ala Phe Asp Leu Ala Arg500 505 510Leu Ala Asn Asp Asp Asp Val Met Leu Leu Asp Trp Val Lys Gly Leu515 520 525Leu Lys Glu Lys Lys Leu Glu Met Leu Val Asp Pro Asp Leu Gln Ser530 535 540Asn Tyr Thr Glu Ala Glu Val Glu Gln Leu Ile Gln Val Ala Leu Leu545 550 555 560Cys Thr Gln Ser Ser Pro Met Glu Arg Pro Lys Met Ser Glu Val Val565 570 575Arg Met Leu Glu Gly Asp Gly Leu Ala Glu Lys Trp Asp Glu Trp Gln580 585 590Lys Val Glu Val Leu Arg Gln Glu Val Glu Leu Ser Ser His Pro Thr595 600 605Ser Asp Trp Ile Leu Asp Ser Thr Asp Asn Leu His Ala Met Glu Leu610 615 620Ser Gly Pro Arg625481920DNAArabidopsis thalianaCDS(79)..(1851)/note="Arabidopsis thaliana RKS10 cDNA" 48atcaggggtt ttaacaatga tggattttct ctgatgaggg atagttctag ggtttgtttt 60taatctcttg aggataaa atg gaa cga aga tta atg atc cct tgc ttc ttt 111Met Glu Arg Arg Leu Met Ile Pro Cys Phe Phe1 5 10tgg ttg att ctc gtt ttg gat ttg gtt ctc aga gtc tcg ggc aac gcc 159Trp Leu Ile Leu Val Leu Asp Leu Val Leu Arg Val Ser Gly Asn Ala15 20 25gaa ggt gat gct cta agt gca ctg aaa aac agt tta gcc gac cct aat 207Glu Gly Asp Ala Leu Ser Ala Leu Lys Asn Ser Leu Ala Asp Pro Asn30 35 40aag gtg ctt caa agt tgg gat gct act ctt gtt act cca tgt aca tgg 255Lys Val Leu Gln Ser Trp Asp Ala Thr Leu Val Thr Pro Cys Thr Trp45 50 55ttt cat gtt act tgc aat agc gac aat agt gtt aca cgt gtt gac ctt 303Phe His Val Thr Cys Asn Ser Asp Asn Ser Val Thr Arg Val Asp Leu60 65 70 75ggg aat gca aat cta tct gga cag ctc gta atg caa ctt ggt cag ctt 351Gly Asn Ala Asn Leu Ser Gly Gln Leu Val Met Gln Leu Gly Gln Leu80 85 90cca aac ttg cag tac ttg gag ctt tat agc aat aac att act ggg aca 399Pro Asn Leu Gln Tyr Leu Glu Leu Tyr Ser Asn Asn Ile Thr Gly Thr95 100 105atc cca gaa cag ctt gga aat ctg acg gaa ttg gtg agc ttg gat ctt 447Ile Pro Glu Gln Leu Gly Asn Leu Thr Glu Leu Val Ser Leu Asp Leu110 115 120tac ttg aac aat tta agc ggg cct att cca tca act ctc ggc cga ctt 495Tyr Leu Asn Asn Leu Ser Gly Pro Ile Pro Ser Thr Leu Gly Arg Leu125 130 135aag aaa ctc cgt ttc ttg cgt ctt aat aac aat agc tta tct gga gaa 543Lys Lys Leu Arg Phe Leu Arg Leu Asn Asn Asn Ser Leu Ser Gly Glu140 145 150 155att cca agg tct ttg act gct gtc ctg acg cta caa gtt ctt ttt gcc 591Ile Pro Arg Ser Leu Thr Ala Val Leu Thr Leu Gln Val Leu Phe Ala160 165 170aac acc aag ttg act ccc ctt cct gca tct cca ccg cct cct atc tct 639Asn Thr Lys Leu Thr Pro Leu Pro Ala Ser Pro Pro Pro Pro Ile Ser175 180 185cct aca ccg cca tca cct gca ggg agt aat aga att act gga gcg att 687Pro Thr Pro Pro Ser Pro Ala Gly Ser Asn Arg Ile Thr Gly Ala Ile190 195 200gcg gga gga gtt gct gca ggt gct gca ctt cta ttt gct gtt ccg gcc 735Ala Gly Gly Val Ala Ala Gly Ala Ala Leu Leu Phe Ala Val Pro Ala205 210 215att gca cta gct tgg tgg cga agg aaa aag ccg cag gac cac ttc ttt 783Ile Ala Leu Ala Trp Trp Arg Arg Lys Lys Pro Gln Asp His Phe Phe220 225 230 235gat gta cca gct gaa gag gac cca gaa gtt cat tta gga caa ctg aag 831Asp Val Pro Ala Glu Glu Asp Pro Glu Val His Leu Gly Gln Leu Lys240 245 250agg ttt tca ttg cgt gaa cta caa gtt gct tcg gat aat ttt agc aac 879Arg Phe Ser Leu Arg Glu Leu Gln Val Ala Ser Asp Asn Phe Ser Asn255 260 265aag aac ata ttg ggt aga ggt ggt ttt ggt aaa gtt tat aaa gga cgg 927Lys Asn Ile Leu Gly Arg Gly Gly Phe Gly Lys Val Tyr Lys Gly Arg270 275 280tta gct gat ggt act tta gtg gcc gtt aaa agg cta aaa gag gag cgc 975Leu

Ala Asp Gly Thr Leu Val Ala Val Lys Arg Leu Lys Glu Glu Arg285 290 295acc caa ggt ggc gaa ctg cag ttc cag aca gag gtt gag atg att agt 1023Thr Gln Gly Gly Glu Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser300 305 310 315atg gcg gtt cac aga aac ttg ctt cgg ctt cgt gga ttt tgc atg act 1071Met Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr320 325 330cca acc gaa aga ttg ctt gtt tat ccc tac atg gct aat gga agt gtt 1119Pro Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val335 340 345gcc tcc tgt tta aga gaa cgt ccc gag tcc cag cca cca ctt gat tgg 1167Ala Ser Cys Leu Arg Glu Arg Pro Glu Ser Gln Pro Pro Leu Asp Trp350 355 360cca aag aga cag cgt att gcg ttg gga tct gca aga ggg ctt gcg tat 1215Pro Lys Arg Gln Arg Ile Ala Leu Gly Ser Ala Arg Gly Leu Ala Tyr365 370 375tta cat gat cat tgc gac cca aag att att cat cga gat gtg aaa gct 1263Leu His Asp His Cys Asp Pro Lys Ile Ile His Arg Asp Val Lys Ala380 385 390 395gca aat att ttg ttg gat gaa gag ttt gaa gcc gtg gtt ggg gat ttt 1311Ala Asn Ile Leu Leu Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe400 405 410gga ctt gca aaa ctc atg gac tac aaa gac aca cat gtg aca acc gca 1359Gly Leu Ala Lys Leu Met Asp Tyr Lys Asp Thr His Val Thr Thr Ala415 420 425gtg cgt ggg aca att ggt cat ata gcc cct gag tac ctt tcc act gga 1407Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly430 435 440aaa tca tca gag aaa acc gat gtc ttt ggg tat gga gtc atg ctt ctt 1455Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Val Met Leu Leu445 450 455gag ctt atc act gga caa agg gct ttt gat ctt gct cgc ctc gcg aat 1503Glu Leu Ile Thr Gly Gln Arg Ala Phe Asp Leu Ala Arg Leu Ala Asn460 465 470 475gat gat gat gtc atg tta cta gac tgg gtg aaa ggg ttg tta aaa gag 1551Asp Asp Asp Val Met Leu Leu Asp Trp Val Lys Gly Leu Leu Lys Glu480 485 490aag aaa ttg gaa gca cta gta gat gtt gat ctt cag ggt aat tac aaa 1599Lys Lys Leu Glu Ala Leu Val Asp Val Asp Leu Gln Gly Asn Tyr Lys495 500 505gac gaa gaa gtg gag cag cta atc caa gtg gct tta ctc tgc act cag 1647Asp Glu Glu Val Glu Gln Leu Ile Gln Val Ala Leu Leu Cys Thr Gln510 515 520agt tca cca atg gaa aga ccc aaa atg tct gaa gtt gta aga atg ctt 1695Ser Ser Pro Met Glu Arg Pro Lys Met Ser Glu Val Val Arg Met Leu525 530 535gaa gga gat ggt tta gct gag aga tgg gaa gag tgg caa aag gag gaa 1743Glu Gly Asp Gly Leu Ala Glu Arg Trp Glu Glu Trp Gln Lys Glu Glu540 545 550 555atg ttc aga caa gat ttc aac tac cca acc cac cat cca gcc gtg tct 1791Met Phe Arg Gln Asp Phe Asn Tyr Pro Thr His His Pro Ala Val Ser560 565 570ggc tgg atc att ggc gat tcc act tcc cag atc gaa aac gaa tac ccc 1839Gly Trp Ile Ile Gly Asp Ser Thr Ser Gln Ile Glu Asn Glu Tyr Pro575 580 585tcg ggt cca aga taagattcga aacacgaatg ttttttctgt attttgtttt 1891Ser Gly Pro Arg590tctctgtatt tattgagggt tttagcttc 192049591PRTArabidopsis thaliana 49Met Glu Arg Arg Leu Met Ile Pro Cys Phe Phe Trp Leu Ile Leu Val1 5 10 15Leu Asp Leu Val Leu Arg Val Ser Gly Asn Ala Glu Gly Asp Ala Leu20 25 30Ser Ala Leu Lys Asn Ser Leu Ala Asp Pro Asn Lys Val Leu Gln Ser35 40 45Trp Asp Ala Thr Leu Val Thr Pro Cys Thr Trp Phe His Val Thr Cys50 55 60Asn Ser Asp Asn Ser Val Thr Arg Val Asp Leu Gly Asn Ala Asn Leu65 70 75 80Ser Gly Gln Leu Val Met Gln Leu Gly Gln Leu Pro Asn Leu Gln Tyr85 90 95Leu Glu Leu Tyr Ser Asn Asn Ile Thr Gly Thr Ile Pro Glu Gln Leu100 105 110Gly Asn Leu Thr Glu Leu Val Ser Leu Asp Leu Tyr Leu Asn Asn Leu115 120 125Ser Gly Pro Ile Pro Ser Thr Leu Gly Arg Leu Lys Lys Leu Arg Phe130 135 140Leu Arg Leu Asn Asn Asn Ser Leu Ser Gly Glu Ile Pro Arg Ser Leu145 150 155 160Thr Ala Val Leu Thr Leu Gln Val Leu Phe Ala Asn Thr Lys Leu Thr165 170 175Pro Leu Pro Ala Ser Pro Pro Pro Pro Ile Ser Pro Thr Pro Pro Ser180 185 190Pro Ala Gly Ser Asn Arg Ile Thr Gly Ala Ile Ala Gly Gly Val Ala195 200 205Ala Gly Ala Ala Leu Leu Phe Ala Val Pro Ala Ile Ala Leu Ala Trp210 215 220Trp Arg Arg Lys Lys Pro Gln Asp His Phe Phe Asp Val Pro Ala Glu225 230 235 240Glu Asp Pro Glu Val His Leu Gly Gln Leu Lys Arg Phe Ser Leu Arg245 250 255Glu Leu Gln Val Ala Ser Asp Asn Phe Ser Asn Lys Asn Ile Leu Gly260 265 270Arg Gly Gly Phe Gly Lys Val Tyr Lys Gly Arg Leu Ala Asp Gly Thr275 280 285Leu Val Ala Val Lys Arg Leu Lys Glu Glu Arg Thr Gln Gly Gly Glu290 295 300Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser Met Ala Val His Arg305 310 315 320Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr Pro Thr Glu Arg Leu325 330 335Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val Ala Ser Cys Leu Arg340 345 350Glu Arg Pro Glu Ser Gln Pro Pro Leu Asp Trp Pro Lys Arg Gln Arg355 360 365Ile Ala Leu Gly Ser Ala Arg Gly Leu Ala Tyr Leu His Asp His Cys370 375 380Asp Pro Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile Leu Leu385 390 395 400Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala Lys Leu405 410 415Met Asp Tyr Lys Asp Thr His Val Thr Thr Ala Val Arg Gly Thr Ile420 425 430Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Lys Ser Ser Glu Lys435 440 445Thr Asp Val Phe Gly Tyr Gly Val Met Leu Leu Glu Leu Ile Thr Gly450 455 460Gln Arg Ala Phe Asp Leu Ala Arg Leu Ala Asn Asp Asp Asp Val Met465 470 475 480Leu Leu Asp Trp Val Lys Gly Leu Leu Lys Glu Lys Lys Leu Glu Ala485 490 495Leu Val Asp Val Asp Leu Gln Gly Asn Tyr Lys Asp Glu Glu Val Glu500 505 510Gln Leu Ile Gln Val Ala Leu Leu Cys Thr Gln Ser Ser Pro Met Glu515 520 525Arg Pro Lys Met Ser Glu Val Val Arg Met Leu Glu Gly Asp Gly Leu530 535 540Ala Glu Arg Trp Glu Glu Trp Gln Lys Glu Glu Met Phe Arg Gln Asp545 550 555 560Phe Asn Tyr Pro Thr His His Pro Ala Val Ser Gly Trp Ile Ile Gly565 570 575Asp Ser Thr Ser Gln Ile Glu Asn Glu Tyr Pro Ser Gly Pro Arg580 585 590501779DNAArabidopsis thalianaCDS(29)..(1747)/note="Arabidopsis thaliana RKS11 cDNA" 50tgttaacctc tcgtaactaa aatcttcc atg aag att caa att cat ctc ctt 52Met Lys Ile Gln Ile His Leu Leu1 5tac tcg ttc ttg ttc ctc tgt ttc tct act ctc act cta tct tct gag 100Tyr Ser Phe Leu Phe Leu Cys Phe Ser Thr Leu Thr Leu Ser Ser Glu10 15 20ccc aga aac cct gaa gtt gag gcg ttg ata agt ata agg aac aat ttg 148Pro Arg Asn Pro Glu Val Glu Ala Leu Ile Ser Ile Arg Asn Asn Leu25 30 35 40cat gat cct cat gga gct ttg aac aat tgg gac gag ttt tca gtt gat 196His Asp Pro His Gly Ala Leu Asn Asn Trp Asp Glu Phe Ser Val Asp45 50 55cct tgt agc tgg gct atg atc act tgc tct ccc gac aac ctc gtc att 244Pro Cys Ser Trp Ala Met Ile Thr Cys Ser Pro Asp Asn Leu Val Ile60 65 70gga ctg tca ttg caa aat aac aac atc tcc ggc aaa att cca ccg gag 292Gly Leu Ser Leu Gln Asn Asn Asn Ile Ser Gly Lys Ile Pro Pro Glu75 80 85ctc ggt ttt cta ccc aaa tta caa acc ttg gat ctt tcc aac aac cga 340Leu Gly Phe Leu Pro Lys Leu Gln Thr Leu Asp Leu Ser Asn Asn Arg90 95 100ttc tcc ggt gac atc cct gtt tcc atc gac cag cta agc agc ctt caa 388Phe Ser Gly Asp Ile Pro Val Ser Ile Asp Gln Leu Ser Ser Leu Gln105 110 115 120tat ctg aga ctc aac aac aac tct ttg tct ggg ccc ttc cct gct tct 436Tyr Leu Arg Leu Asn Asn Asn Ser Leu Ser Gly Pro Phe Pro Ala Ser125 130 135ttg tcc caa att cct cac ctc tcc ttc ttg gac ttg tct tac aac aat 484Leu Ser Gln Ile Pro His Leu Ser Phe Leu Asp Leu Ser Tyr Asn Asn140 145 150ctc agt ggc cct gtt cct aaa ttc cca gca agg act ttc aac gtt gct 532Leu Ser Gly Pro Val Pro Lys Phe Pro Ala Arg Thr Phe Asn Val Ala155 160 165ggt aat cct ttg att tgt aga agc aac cca cct gag att tgt tct gga 580Gly Asn Pro Leu Ile Cys Arg Ser Asn Pro Pro Glu Ile Cys Ser Gly170 175 180tca atc aat gca agt cca ctt tct gtt tct ttg agc tct tca tca gca 628Ser Ile Asn Ala Ser Pro Leu Ser Val Ser Leu Ser Ser Ser Ser Ala185 190 195 200gat aaa caa gag gaa ggg ctt caa gga ctt ggg aat cta aga agc ttc 676Asp Lys Gln Glu Glu Gly Leu Gln Gly Leu Gly Asn Leu Arg Ser Phe205 210 215aca ttc aga gaa ctc cat gtt tat aca gat ggt ttc agt tcc aag aac 724Thr Phe Arg Glu Leu His Val Tyr Thr Asp Gly Phe Ser Ser Lys Asn220 225 230att ctc ggc gct ggt gga ttc ggt aat gtg tac aga ggc aag ctt gga 772Ile Leu Gly Ala Gly Gly Phe Gly Asn Val Tyr Arg Gly Lys Leu Gly235 240 245gat ggg aca atg gtg gca gtg aaa cgg ttg aag gat att aat gga acc 820Asp Gly Thr Met Val Ala Val Lys Arg Leu Lys Asp Ile Asn Gly Thr250 255 260tca ggg gat tca cag ttt cgt atg gag cta gag atg att agc tta gct 868Ser Gly Asp Ser Gln Phe Arg Met Glu Leu Glu Met Ile Ser Leu Ala265 270 275 280gtt cat aag aat ctg ctt cgg tta att ggt tat tgc gca act tct ggt 916Val His Lys Asn Leu Leu Arg Leu Ile Gly Tyr Cys Ala Thr Ser Gly285 290 295gaa agg ctt ctt gtt tac cct tac atg cct aat gga agc gtc gcc tct 964Glu Arg Leu Leu Val Tyr Pro Tyr Met Pro Asn Gly Ser Val Ala Ser300 305 310aag ctt aaa tct aaa ccg gca ttg gac tgg aac atg agg aag agg ata 1012Lys Leu Lys Ser Lys Pro Ala Leu Asp Trp Asn Met Arg Lys Arg Ile315 320 325gca att ggt gca gcg aga ggt ttg ttg tat cta cat gag caa tgt gat 1060Ala Ile Gly Ala Ala Arg Gly Leu Leu Tyr Leu His Glu Gln Cys Asp330 335 340ccc aag atc att cat aga gat gta aag gca gct aat att ctc tta gac 1108Pro Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile Leu Leu Asp345 350 355 360gag tgc ttt gaa gct gtt gtt ggt gac ttt gga ctc gca aag ctc ctt 1156Glu Cys Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala Lys Leu Leu365 370 375aac cat gcg gat tct cat gtc aca act gcg gtc cgt ggt acg gtt ggc 1204Asn His Ala Asp Ser His Val Thr Thr Ala Val Arg Gly Thr Val Gly380 385 390cac att gca cct gaa tat ctc tcc act ggt cag tct tct gag aaa acc 1252His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Gln Ser Ser Glu Lys Thr395 400 405gat gtg ttt ggg ttc ggt ata cta ttg ctc gag ctc ata acc gga ctg 1300Asp Val Phe Gly Phe Gly Ile Leu Leu Leu Glu Leu Ile Thr Gly Leu410 415 420aga gct ctt gag ttt ggt aaa acc gtt agc cag aaa gga gct atg ctt 1348Arg Ala Leu Glu Phe Gly Lys Thr Val Ser Gln Lys Gly Ala Met Leu425 430 435 440gaa tgg gtg agg aaa tta cat gaa gag atg aaa gta gag gaa cta ttg 1396Glu Trp Val Arg Lys Leu His Glu Glu Met Lys Val Glu Glu Leu Leu445 450 455gat cga gaa ctc gga act aac tac gat aag att gaa gtt gga gag atg 1444Asp Arg Glu Leu Gly Thr Asn Tyr Asp Lys Ile Glu Val Gly Glu Met460 465 470ttg caa gtg gct ttg cta tgc aca caa tat ctg cca gct cat cgt cct 1492Leu Gln Val Ala Leu Leu Cys Thr Gln Tyr Leu Pro Ala His Arg Pro475 480 485aaa atg tct gaa gtt gtt ttg atg ctt gaa ggc gat gga tta gcc gag 1540Lys Met Ser Glu Val Val Leu Met Leu Glu Gly Asp Gly Leu Ala Glu490 495 500aga tgg gct gct tcg cat aac cat tca cat ttc tac cat gcc aat atc 1588Arg Trp Ala Ala Ser His Asn His Ser His Phe Tyr His Ala Asn Ile505 510 515 520tct ttc aag aca atc tct tct ctg tct act act tct gtc tca agg ctt 1636Ser Phe Lys Thr Ile Ser Ser Leu Ser Thr Thr Ser Val Ser Arg Leu525 530 535gac gca cat tgc aat gat cca act tat caa atg ttt gga tct tcg gct 1684Asp Ala His Cys Asn Asp Pro Thr Tyr Gln Met Phe Gly Ser Ser Ala540 545 550ttc gat gat gac gat gat cat cag cct tta gat tcc ttt gcc atg gaa 1732Phe Asp Asp Asp Asp Asp His Gln Pro Leu Asp Ser Phe Ala Met Glu555 560 565cta tcc ggt cca aga taacacaatg aaaaaaaaaa aaaaaaaaaa aa 1779Leu Ser Gly Pro Arg570 51573PRTArabidopsis thaliana 51Met Lys Ile Gln Ile His Leu Leu Tyr Ser Phe Leu Phe Leu Cys Phe1 5 10 15Ser Thr Leu Thr Leu Ser Ser Glu Pro Arg Asn Pro Glu Val Glu Ala20 25 30Leu Ile Ser Ile Arg Asn Asn Leu His Asp Pro His Gly Ala Leu Asn35 40 45Asn Trp Asp Glu Phe Ser Val Asp Pro Cys Ser Trp Ala Met Ile Thr50 55 60Cys Ser Pro Asp Asn Leu Val Ile Gly Leu Ser Leu Gln Asn Asn Asn65 70 75 80Ile Ser Gly Lys Ile Pro Pro Glu Leu Gly Phe Leu Pro Lys Leu Gln85 90 95Thr Leu Asp Leu Ser Asn Asn Arg Phe Ser Gly Asp Ile Pro Val Ser100 105 110Ile Asp Gln Leu Ser Ser Leu Gln Tyr Leu Arg Leu Asn Asn Asn Ser115 120 125Leu Ser Gly Pro Phe Pro Ala Ser Leu Ser Gln Ile Pro His Leu Ser130 135 140Phe Leu Asp Leu Ser Tyr Asn Asn Leu Ser Gly Pro Val Pro Lys Phe145 150 155 160Pro Ala Arg Thr Phe Asn Val Ala Gly Asn Pro Leu Ile Cys Arg Ser165 170 175Asn Pro Pro Glu Ile Cys Ser Gly Ser Ile Asn Ala Ser Pro Leu Ser180 185 190Val Ser Leu Ser Ser Ser Ser Ala Asp Lys Gln Glu Glu Gly Leu Gln195 200 205Gly Leu Gly Asn Leu Arg Ser Phe Thr Phe Arg Glu Leu His Val Tyr210 215 220Thr Asp Gly Phe Ser Ser Lys Asn Ile Leu Gly Ala Gly Gly Phe Gly225 230 235 240Asn Val Tyr Arg Gly Lys Leu Gly Asp Gly Thr Met Val Ala Val Lys245 250 255Arg Leu Lys Asp Ile Asn Gly Thr Ser Gly Asp Ser Gln Phe Arg Met260 265 270Glu Leu Glu Met Ile Ser Leu Ala Val His Lys Asn Leu Leu Arg Leu275 280 285Ile Gly Tyr Cys Ala Thr Ser Gly Glu Arg Leu Leu Val Tyr Pro Tyr290 295 300Met Pro Asn Gly Ser Val Ala Ser Lys Leu Lys Ser Lys Pro Ala Leu305 310 315 320Asp Trp Asn Met Arg Lys Arg Ile Ala Ile Gly Ala Ala Arg Gly Leu325 330 335Leu Tyr Leu His Glu Gln Cys Asp Pro Lys Ile Ile His Arg Asp Val340 345 350Lys Ala Ala Asn Ile Leu Leu Asp Glu Cys Phe Glu Ala Val Val Gly355 360 365Asp Phe Gly Leu Ala Lys Leu Leu Asn His Ala Asp Ser His Val Thr370 375 380Thr Ala Val Arg Gly Thr Val Gly His Ile Ala Pro Glu Tyr Leu Ser385 390 395 400Thr Gly Gln Ser Ser Glu Lys Thr Asp Val Phe Gly Phe Gly Ile Leu405 410 415Leu Leu Glu Leu Ile Thr Gly Leu Arg Ala Leu Glu Phe Gly Lys Thr420 425 430Val Ser Gln Lys Gly Ala Met Leu Glu Trp Val Arg Lys Leu His Glu435 440 445Glu Met Lys Val Glu Glu Leu Leu Asp Arg Glu Leu Gly Thr Asn Tyr450 455 460Asp Lys Ile Glu Val Gly Glu Met Leu Gln Val Ala Leu Leu Cys Thr465 470 475 480Gln Tyr Leu Pro Ala His Arg Pro Lys Met Ser Glu Val Val Leu Met485 490 495Leu Glu Gly Asp Gly Leu Ala Glu Arg Trp Ala Ala Ser His Asn His500 505 510Ser His Phe Tyr His Ala Asn Ile Ser Phe Lys Thr Ile Ser Ser Leu515 520 525Ser Thr Thr Ser Val Ser Arg Leu Asp Ala His Cys Asn Asp Pro Thr530 535 540Tyr Gln Met Phe Gly Ser Ser Ala Phe Asp Asp Asp Asp Asp His Gln545 550 555 560Pro Leu Asp Ser Phe Ala Met Glu Leu Ser Gly Pro Arg565

570521925DNAArabidopsis thalianaCDS(61)..(1791)/note="Arabidopsis thaliana RKS12 cDNA" 52tttaaaaacc ttgctagttc tcaattctca tgactttgct tttagtctta gaagtggaaa 60atg gaa cat gga tca tcc cgt ggc ttt att tgg ctg att cta ttt ctc 108Met Glu His Gly Ser Ser Arg Gly Phe Ile Trp Leu Ile Leu Phe Leu1 5 10 15gat ttt gtt tcc aga gtc acc gga aaa aca caa gtt gat gct ctc att 156Asp Phe Val Ser Arg Val Thr Gly Lys Thr Gln Val Asp Ala Leu Ile20 25 30gct cta aga agc agt tta tca tca ggt gac cat aca aac aat ata ctc 204Ala Leu Arg Ser Ser Leu Ser Ser Gly Asp His Thr Asn Asn Ile Leu35 40 45caa agc tgg aat gcc act cac gtt act cca tgt tca tgg ttt cat gtt 252Gln Ser Trp Asn Ala Thr His Val Thr Pro Cys Ser Trp Phe His Val50 55 60act tgc aat act gaa aac agt gtt act cgt ctg gaa ctt ttt aac aat 300Thr Cys Asn Thr Glu Asn Ser Val Thr Arg Leu Glu Leu Phe Asn Asn65 70 75 80aat att act ggg gag ata cct gag gag ctt ggc gac ttg atg gaa cta 348Asn Ile Thr Gly Glu Ile Pro Glu Glu Leu Gly Asp Leu Met Glu Leu85 90 95gta agc ttg gac ctt ttt gca aac aac ata agc ggt ccc atc cct tcc 396Val Ser Leu Asp Leu Phe Ala Asn Asn Ile Ser Gly Pro Ile Pro Ser100 105 110tct ctt ggc aaa cta gga aaa ctc cgc ttc ttg cgt ctt tat aac aac 444Ser Leu Gly Lys Leu Gly Lys Leu Arg Phe Leu Arg Leu Tyr Asn Asn115 120 125agc tta tct gga gaa att cca agg tct ttg act gct ctg ccg ctg gat 492Ser Leu Ser Gly Glu Ile Pro Arg Ser Leu Thr Ala Leu Pro Leu Asp130 135 140gtt ctt gat atc tca aac aat cgg ctc agt gga gat att cct gtt aat 540Val Leu Asp Ile Ser Asn Asn Arg Leu Ser Gly Asp Ile Pro Val Asn145 150 155 160ggt tcc ttt tcg cag ttc act tct atg agt ttt gcc aat aat aaa tta 588Gly Ser Phe Ser Gln Phe Thr Ser Met Ser Phe Ala Asn Asn Lys Leu165 170 175agg ccg cga cct gca tct cct tca cca tca cct tca gga acg tct gca 636Arg Pro Arg Pro Ala Ser Pro Ser Pro Ser Pro Ser Gly Thr Ser Ala180 185 190gca ata gta gtg gga gtt gct gcg ggt gca gca ctt cta ttt gcg ctt 684Ala Ile Val Val Gly Val Ala Ala Gly Ala Ala Leu Leu Phe Ala Leu195 200 205gct tgg tgg ctg aga aga aaa ctg cag ggt cac ttt ctt gat gta cct 732Ala Trp Trp Leu Arg Arg Lys Leu Gln Gly His Phe Leu Asp Val Pro210 215 220gct gaa gaa gac cca gag gtt tat tta gga caa ttt aaa agg ttc tcc 780Ala Glu Glu Asp Pro Glu Val Tyr Leu Gly Gln Phe Lys Arg Phe Ser225 230 235 240ttg cgt gaa ctg cta gtt gct aca gag aaa ttt agc aaa aga aat gta 828Leu Arg Glu Leu Leu Val Ala Thr Glu Lys Phe Ser Lys Arg Asn Val245 250 255ttg ggc aaa gga cgt ttt ggt ata ttg tat aaa gga cgt tta gct gat 876Leu Gly Lys Gly Arg Phe Gly Ile Leu Tyr Lys Gly Arg Leu Ala Asp260 265 270gac act cta gtg gct gtg aaa cgg cta aat gaa gaa cgt acc aag ggt 924Asp Thr Leu Val Ala Val Lys Arg Leu Asn Glu Glu Arg Thr Lys Gly275 280 285ggg gaa ctg cag ttt caa acc gaa gtt gag atg atc agt atg gcc gtt 972Gly Glu Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser Met Ala Val290 295 300cat agg aac ttg ctt cgg ctt cgt ggc ttt tgc atg act cca act gaa 1020His Arg Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr Pro Thr Glu305 310 315 320aga tta ctt gtt tat ccc tac atg gct aat gga agt gtt gct tct tgt 1068Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val Ala Ser Cys325 330 335tta aga gag cgt cct gaa ggc aat cca gcc ctt gac tgg cca aaa aga 1116Leu Arg Glu Arg Pro Glu Gly Asn Pro Ala Leu Asp Trp Pro Lys Arg340 345 350aag cat att gct ctg gga tca gca agg ggg ctc gca tat tta cac gat 1164Lys His Ile Ala Leu Gly Ser Ala Arg Gly Leu Ala Tyr Leu His Asp355 360 365cat tgc gac caa aag atc att cac ctg gat gtg aaa gct gca aat ata 1212His Cys Asp Gln Lys Ile Ile His Leu Asp Val Lys Ala Ala Asn Ile370 375 380ctg tta gat gaa gag ttt gaa gct gtt gtt gga gat ttt ggg cta gca 1260Leu Leu Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala385 390 395 400aaa tta atg aat tat aac gac tcc cat gtg aca act gct gta cgg ggt 1308Lys Leu Met Asn Tyr Asn Asp Ser His Val Thr Thr Ala Val Arg Gly405 410 415acg att ggc cat ata gcg ccc gag tac ctc tcg aca gga aaa tct tct 1356Thr Ile Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Lys Ser Ser420 425 430gag aag act gat gtt ttt ggg tac ggg gtc atg ctt ctc gag ctc atc 1404Glu Lys Thr Asp Val Phe Gly Tyr Gly Val Met Leu Leu Glu Leu Ile435 440 445act gga caa aag gct ttc gat ctt gct cgg ctt gca aat gat gat gat 1452Thr Gly Gln Lys Ala Phe Asp Leu Ala Arg Leu Ala Asn Asp Asp Asp450 455 460atc atg tta ctc gac tgg gtg aaa gag gtt ttg aaa gag aag aag ttg 1500Ile Met Leu Leu Asp Trp Val Lys Glu Val Leu Lys Glu Lys Lys Leu465 470 475 480gaa agc ctt gtg gat gca gaa ctc gaa gga aag tac gtg gaa aca gaa 1548Glu Ser Leu Val Asp Ala Glu Leu Glu Gly Lys Tyr Val Glu Thr Glu485 490 495gtg gag cag ctg ata caa atg gct ctg ctc tgc act caa agt tct gca 1596Val Glu Gln Leu Ile Gln Met Ala Leu Leu Cys Thr Gln Ser Ser Ala500 505 510atg gaa cgt cca aag atg tca gaa gta gtg aga atg ctg gaa gga gat 1644Met Glu Arg Pro Lys Met Ser Glu Val Val Arg Met Leu Glu Gly Asp515 520 525ggt tta gct gag aga tgg gaa gaa tgg caa aag gag gag atg cca ata 1692Gly Leu Ala Glu Arg Trp Glu Glu Trp Gln Lys Glu Glu Met Pro Ile530 535 540cat gat ttt aac tat caa gcc tat cct cat gct ggc act gac tgg ctc 1740His Asp Phe Asn Tyr Gln Ala Tyr Pro His Ala Gly Thr Asp Trp Leu545 550 555 560atc ccc tat tcc aat tcc ctt atc gaa aac gat tac ccc tcg ggg cca 1788Ile Pro Tyr Ser Asn Ser Leu Ile Glu Asn Asp Tyr Pro Ser Gly Pro565 570 575aga taacctttta gaaagggtca tttcttgtgg gttcttcaac aagtatatat 1841Argataggtagtg aagttgtaag aagcaaaacc ccacattcac ctttgaatat cactactcta 1901taaaaaaaaa aaaaaaaaaa aaaa 192553577PRTArabidopsis thaliana 53Met Glu His Gly Ser Ser Arg Gly Phe Ile Trp Leu Ile Leu Phe Leu1 5 10 15Asp Phe Val Ser Arg Val Thr Gly Lys Thr Gln Val Asp Ala Leu Ile20 25 30Ala Leu Arg Ser Ser Leu Ser Ser Gly Asp His Thr Asn Asn Ile Leu35 40 45Gln Ser Trp Asn Ala Thr His Val Thr Pro Cys Ser Trp Phe His Val50 55 60Thr Cys Asn Thr Glu Asn Ser Val Thr Arg Leu Glu Leu Phe Asn Asn65 70 75 80Asn Ile Thr Gly Glu Ile Pro Glu Glu Leu Gly Asp Leu Met Glu Leu85 90 95Val Ser Leu Asp Leu Phe Ala Asn Asn Ile Ser Gly Pro Ile Pro Ser100 105 110Ser Leu Gly Lys Leu Gly Lys Leu Arg Phe Leu Arg Leu Tyr Asn Asn115 120 125Ser Leu Ser Gly Glu Ile Pro Arg Ser Leu Thr Ala Leu Pro Leu Asp130 135 140Val Leu Asp Ile Ser Asn Asn Arg Leu Ser Gly Asp Ile Pro Val Asn145 150 155 160Gly Ser Phe Ser Gln Phe Thr Ser Met Ser Phe Ala Asn Asn Lys Leu165 170 175Arg Pro Arg Pro Ala Ser Pro Ser Pro Ser Pro Ser Gly Thr Ser Ala180 185 190Ala Ile Val Val Gly Val Ala Ala Gly Ala Ala Leu Leu Phe Ala Leu195 200 205Ala Trp Trp Leu Arg Arg Lys Leu Gln Gly His Phe Leu Asp Val Pro210 215 220Ala Glu Glu Asp Pro Glu Val Tyr Leu Gly Gln Phe Lys Arg Phe Ser225 230 235 240Leu Arg Glu Leu Leu Val Ala Thr Glu Lys Phe Ser Lys Arg Asn Val245 250 255Leu Gly Lys Gly Arg Phe Gly Ile Leu Tyr Lys Gly Arg Leu Ala Asp260 265 270Asp Thr Leu Val Ala Val Lys Arg Leu Asn Glu Glu Arg Thr Lys Gly275 280 285Gly Glu Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser Met Ala Val290 295 300His Arg Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr Pro Thr Glu305 310 315 320Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val Ala Ser Cys325 330 335Leu Arg Glu Arg Pro Glu Gly Asn Pro Ala Leu Asp Trp Pro Lys Arg340 345 350Lys His Ile Ala Leu Gly Ser Ala Arg Gly Leu Ala Tyr Leu His Asp355 360 365His Cys Asp Gln Lys Ile Ile His Leu Asp Val Lys Ala Ala Asn Ile370 375 380Leu Leu Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala385 390 395 400Lys Leu Met Asn Tyr Asn Asp Ser His Val Thr Thr Ala Val Arg Gly405 410 415Thr Ile Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Lys Ser Ser420 425 430Glu Lys Thr Asp Val Phe Gly Tyr Gly Val Met Leu Leu Glu Leu Ile435 440 445Thr Gly Gln Lys Ala Phe Asp Leu Ala Arg Leu Ala Asn Asp Asp Asp450 455 460Ile Met Leu Leu Asp Trp Val Lys Glu Val Leu Lys Glu Lys Lys Leu465 470 475 480Glu Ser Leu Val Asp Ala Glu Leu Glu Gly Lys Tyr Val Glu Thr Glu485 490 495Val Glu Gln Leu Ile Gln Met Ala Leu Leu Cys Thr Gln Ser Ser Ala500 505 510Met Glu Arg Pro Lys Met Ser Glu Val Val Arg Met Leu Glu Gly Asp515 520 525Gly Leu Ala Glu Arg Trp Glu Glu Trp Gln Lys Glu Glu Met Pro Ile530 535 540His Asp Phe Asn Tyr Gln Ala Tyr Pro His Ala Gly Thr Asp Trp Leu545 550 555 560Ile Pro Tyr Ser Asn Ser Leu Ile Glu Asn Asp Tyr Pro Ser Gly Pro565 570 575Arg541910DNAArabidopsis thalianaCDS(40)..(1827)/note="Arabidopsis thaliana RKS13 cDNA" 54taataaacct ctaataataa tggctttgct tttactctg atg aca agt tca aaa 54Met Thr Ser Ser Lys1 5atg gaa caa aga tca ctc ctt tgc ttc ctt tat ctg ctc cta cta ttc 102Met Glu Gln Arg Ser Leu Leu Cys Phe Leu Tyr Leu Leu Leu Leu Phe10 15 20aat ttc act ctc aga gtc gct gga aac gct gaa ggt gat gct ttg act 150Asn Phe Thr Leu Arg Val Ala Gly Asn Ala Glu Gly Asp Ala Leu Thr25 30 35cag ctg aaa aac agt ttg tca tca ggt gac cct gca aac aat gta ctc 198Gln Leu Lys Asn Ser Leu Ser Ser Gly Asp Pro Ala Asn Asn Val Leu40 45 50caa agc tgg gat gct act ctt gtt act cca tgt act tgg ttt cat gtt 246Gln Ser Trp Asp Ala Thr Leu Val Thr Pro Cys Thr Trp Phe His Val55 60 65act tgc aat cct gag aat aaa gtt act cgt gtg gag ctt tat agc aat 294Thr Cys Asn Pro Glu Asn Lys Val Thr Arg Val Glu Leu Tyr Ser Asn70 75 80 85aac att aca ggg gag ata cct gag gag ctt ggc gac ttg gtg gaa cta 342Asn Ile Thr Gly Glu Ile Pro Glu Glu Leu Gly Asp Leu Val Glu Leu90 95 100gta agc ttg gat ctt tac gca aac agc ata agc ggt ccc atc cct tcg 390Val Ser Leu Asp Leu Tyr Ala Asn Ser Ile Ser Gly Pro Ile Pro Ser105 110 115tct ctt ggc aaa cta gga aaa ctc cgg ttc ttg cgt ctt aac aac aat 438Ser Leu Gly Lys Leu Gly Lys Leu Arg Phe Leu Arg Leu Asn Asn Asn120 125 130agc tta tca ggg gaa att cca atg act ttg act tct gtg cag ctg caa 486Ser Leu Ser Gly Glu Ile Pro Met Thr Leu Thr Ser Val Gln Leu Gln135 140 145gtt ctg gat atc tca aac aat cgg ctc agt gga gat att cct gtt aat 534Val Leu Asp Ile Ser Asn Asn Arg Leu Ser Gly Asp Ile Pro Val Asn150 155 160 165ggt tct ttt tcg ctc ttc act cct atc agt ttt gcg aat aat agc tta 582Gly Ser Phe Ser Leu Phe Thr Pro Ile Ser Phe Ala Asn Asn Ser Leu170 175 180acg gat ctt ccc gaa cct ccg cct act tct acc tct cct acg cca cca 630Thr Asp Leu Pro Glu Pro Pro Pro Thr Ser Thr Ser Pro Thr Pro Pro185 190 195cca cct tca ggg ggg caa atg act gca gca ata gca ggg gga gtt gct 678Pro Pro Ser Gly Gly Gln Met Thr Ala Ala Ile Ala Gly Gly Val Ala200 205 210gca ggt gca gca ctt cta ttt gct gtt cca gcc att gcg ttt gct tgg 726Ala Gly Ala Ala Leu Leu Phe Ala Val Pro Ala Ile Ala Phe Ala Trp215 220 225tgg ctc aga aga aaa cca cag gac cac ttt ttt gat gta cct gct gaa 774Trp Leu Arg Arg Lys Pro Gln Asp His Phe Phe Asp Val Pro Ala Glu230 235 240 245gaa gac cca gag gtt cat tta gga caa ctc aaa agg ttt acc ttg cgt 822Glu Asp Pro Glu Val His Leu Gly Gln Leu Lys Arg Phe Thr Leu Arg250 255 260gaa ctg tta gtt gct act gat aac ttt agc aat aaa aat gta ttg ggt 870Glu Leu Leu Val Ala Thr Asp Asn Phe Ser Asn Lys Asn Val Leu Gly265 270 275aga ggt ggt ttt ggt aaa gtg tat aaa gga cgt tta gcc gat ggc aat 918Arg Gly Gly Phe Gly Lys Val Tyr Lys Gly Arg Leu Ala Asp Gly Asn280 285 290cta gtg gct gtc aaa agg cta aaa gaa gaa cgt acc aag ggt ggg gaa 966Leu Val Ala Val Lys Arg Leu Lys Glu Glu Arg Thr Lys Gly Gly Glu295 300 305ctg cag ttt caa acc gaa gtt gag atg atc agt atg gcc gtt cat agg 1014Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser Met Ala Val His Arg310 315 320 325aac ttg ctt cgg ctt cgt ggc ttt tgc atg act cca act gaa aga tta 1062Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr Pro Thr Glu Arg Leu330 335 340ctt gtt tat ccc tac atg gct aat gga agt gtt gct tct tgt tta aga 1110Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val Ala Ser Cys Leu Arg345 350 355gag cgt cct gaa ggc aat cca gca ctt gat tgg cca aaa aga aag cat 1158Glu Arg Pro Glu Gly Asn Pro Ala Leu Asp Trp Pro Lys Arg Lys His360 365 370att gct ctg gga tca gca agg ggg ctt gcg tat tta cat gat cat tgc 1206Ile Ala Leu Gly Ser Ala Arg Gly Leu Ala Tyr Leu His Asp His Cys375 380 385gac caa aaa atc att cac cgg gat gtt aaa gct gct aat ata ttg tta 1254Asp Gln Lys Ile Ile His Arg Asp Val Lys Ala Ala Asn Ile Leu Leu390 395 400 405gat gaa gag ttt gaa gct gtt gtt gga gat ttt ggg ctc gca aaa tta 1302Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe Gly Leu Ala Lys Leu410 415 420atg aat tat aat gac tcc cat gtg aca act gct gta cgc ggt aca att 1350Met Asn Tyr Asn Asp Ser His Val Thr Thr Ala Val Arg Gly Thr Ile425 430 435ggc cat ata gcg ccc gag tac ctc tcg aca gga aaa tct tct gag aag 1398Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly Lys Ser Ser Glu Lys440 445 450act gat gtt ttt ggg tac ggg gtc atg ctt ctc gag ctc atc act gga 1446Thr Asp Val Phe Gly Tyr Gly Val Met Leu Leu Glu Leu Ile Thr Gly455 460 465caa aag gct ttc gat ctt gct cgg ctt gca aat gat gat gat atc atg 1494Gln Lys Ala Phe Asp Leu Ala Arg Leu Ala Asn Asp Asp Asp Ile Met470 475 480 485tta ctc gac tgg gtg aaa gag gtt ttg aaa gag aag aag ttg gaa agc 1542Leu Leu Asp Trp Val Lys Glu Val Leu Lys Glu Lys Lys Leu Glu Ser490 495 500ctt gtg gat gca gaa ctc gaa gga aag tac gtg gaa aca gaa gtg gag 1590Leu Val Asp Ala Glu Leu Glu Gly Lys Tyr Val Glu Thr Glu Val Glu505 510 515cag ctg ata caa atg gct ctg ctc tgc act caa agt tct gca atg gaa 1638Gln Leu Ile Gln Met Ala Leu Leu Cys Thr Gln Ser Ser Ala Met Glu520 525 530cgt cca aag atg tca gaa gta gtg aga atg ctg gaa gga gat ggt tta 1686Arg Pro Lys Met Ser Glu Val Val Arg Met Leu Glu Gly Asp Gly Leu535 540 545gct gag aga tgg gaa gaa tgg caa aag gag gag atg cca ata cat gat 1734Ala Glu Arg Trp Glu Glu Trp Gln Lys Glu Glu Met Pro Ile His Asp550 555 560 565ttt aac tat caa gcc tat cct cat gct ggc act gac tgg ctc atc ccc 1782Phe Asn Tyr Gln Ala Tyr Pro His Ala Gly Thr Asp Trp Leu Ile Pro570 575 580tat tcc aat tcc ctt atc gaa aac gat tac ccc tcg ggt cca aga 1827Tyr Ser Asn Ser Leu Ile Glu Asn Asp Tyr Pro Ser Gly Pro Arg585 590 595taacctttta gaaagggtct tttcttgtgg gttcttcaac aagtatatat atagattggt 1887gaagttttaa gatgcaaaaa aaa 191055596PRTArabidopsis thaliana 55Met Thr Ser Ser Lys Met Glu Gln Arg Ser Leu Leu Cys Phe Leu Tyr1 5 10 15Leu Leu Leu Leu Phe Asn Phe Thr Leu Arg Val Ala Gly Asn Ala

Glu20 25 30Gly Asp Ala Leu Thr Gln Leu Lys Asn Ser Leu Ser Ser Gly Asp Pro35 40 45Ala Asn Asn Val Leu Gln Ser Trp Asp Ala Thr Leu Val Thr Pro Cys50 55 60Thr Trp Phe His Val Thr Cys Asn Pro Glu Asn Lys Val Thr Arg Val65 70 75 80Glu Leu Tyr Ser Asn Asn Ile Thr Gly Glu Ile Pro Glu Glu Leu Gly85 90 95Asp Leu Val Glu Leu Val Ser Leu Asp Leu Tyr Ala Asn Ser Ile Ser100 105 110Gly Pro Ile Pro Ser Ser Leu Gly Lys Leu Gly Lys Leu Arg Phe Leu115 120 125Arg Leu Asn Asn Asn Ser Leu Ser Gly Glu Ile Pro Met Thr Leu Thr130 135 140Ser Val Gln Leu Gln Val Leu Asp Ile Ser Asn Asn Arg Leu Ser Gly145 150 155 160Asp Ile Pro Val Asn Gly Ser Phe Ser Leu Phe Thr Pro Ile Ser Phe165 170 175Ala Asn Asn Ser Leu Thr Asp Leu Pro Glu Pro Pro Pro Thr Ser Thr180 185 190Ser Pro Thr Pro Pro Pro Pro Ser Gly Gly Gln Met Thr Ala Ala Ile195 200 205Ala Gly Gly Val Ala Ala Gly Ala Ala Leu Leu Phe Ala Val Pro Ala210 215 220Ile Ala Phe Ala Trp Trp Leu Arg Arg Lys Pro Gln Asp His Phe Phe225 230 235 240Asp Val Pro Ala Glu Glu Asp Pro Glu Val His Leu Gly Gln Leu Lys245 250 255Arg Phe Thr Leu Arg Glu Leu Leu Val Ala Thr Asp Asn Phe Ser Asn260 265 270Lys Asn Val Leu Gly Arg Gly Gly Phe Gly Lys Val Tyr Lys Gly Arg275 280 285Leu Ala Asp Gly Asn Leu Val Ala Val Lys Arg Leu Lys Glu Glu Arg290 295 300Thr Lys Gly Gly Glu Leu Gln Phe Gln Thr Glu Val Glu Met Ile Ser305 310 315 320Met Ala Val His Arg Asn Leu Leu Arg Leu Arg Gly Phe Cys Met Thr325 330 335Pro Thr Glu Arg Leu Leu Val Tyr Pro Tyr Met Ala Asn Gly Ser Val340 345 350Ala Ser Cys Leu Arg Glu Arg Pro Glu Gly Asn Pro Ala Leu Asp Trp355 360 365Pro Lys Arg Lys His Ile Ala Leu Gly Ser Ala Arg Gly Leu Ala Tyr370 375 380Leu His Asp His Cys Asp Gln Lys Ile Ile His Arg Asp Val Lys Ala385 390 395 400Ala Asn Ile Leu Leu Asp Glu Glu Phe Glu Ala Val Val Gly Asp Phe405 410 415Gly Leu Ala Lys Leu Met Asn Tyr Asn Asp Ser His Val Thr Thr Ala420 425 430Val Arg Gly Thr Ile Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly435 440 445Lys Ser Ser Glu Lys Thr Asp Val Phe Gly Tyr Gly Val Met Leu Leu450 455 460Glu Leu Ile Thr Gly Gln Lys Ala Phe Asp Leu Ala Arg Leu Ala Asn465 470 475 480Asp Asp Asp Ile Met Leu Leu Asp Trp Val Lys Glu Val Leu Lys Glu485 490 495Lys Lys Leu Glu Ser Leu Val Asp Ala Glu Leu Glu Gly Lys Tyr Val500 505 510Glu Thr Glu Val Glu Gln Leu Ile Gln Met Ala Leu Leu Cys Thr Gln515 520 525Ser Ser Ala Met Glu Arg Pro Lys Met Ser Glu Val Val Arg Met Leu530 535 540Glu Gly Asp Gly Leu Ala Glu Arg Trp Glu Glu Trp Gln Lys Glu Glu545 550 555 560Met Pro Ile His Asp Phe Asn Tyr Gln Ala Tyr Pro His Ala Gly Thr565 570 575Asp Trp Leu Ile Pro Tyr Ser Asn Ser Leu Ile Glu Asn Asp Tyr Pro580 585 590Ser Gly Pro Arg595561899DNAArabidopsis thalianaCDS(55)..(1857)/note="Arabidopsis thaliana RKS14 cDNA" 56ctgcacctta gagattaata ctctcaagaa aaacaagttt tgattcggac aaag atg 57Met1ttg caa gga aga aga gaa gca aaa aag agt tat gct ttg ttc tct tca 105Leu Gln Gly Arg Arg Glu Ala Lys Lys Ser Tyr Ala Leu Phe Ser Ser5 10 15act ttc ttc ttc ttc ttt atc tgt ttt ctt tct tct tct tct gca gaa 153Thr Phe Phe Phe Phe Phe Ile Cys Phe Leu Ser Ser Ser Ser Ala Glu20 25 30ctc aca gac aaa gtt gtt gcc tta ata gga atc aaa agc tca ctg act 201Leu Thr Asp Lys Val Val Ala Leu Ile Gly Ile Lys Ser Ser Leu Thr35 40 45gat cct cat gga gtt cta atg aat tgg gat gac aca gca gtt gat cca 249Asp Pro His Gly Val Leu Met Asn Trp Asp Asp Thr Ala Val Asp Pro50 55 60 65tgt agc tgg aac atg atc act tgt tct gat ggt ttt gtc ata agg cta 297Cys Ser Trp Asn Met Ile Thr Cys Ser Asp Gly Phe Val Ile Arg Leu70 75 80tac agg tta ttg cag aac aat tac ata aca gga aac atc cct cat gag 345Tyr Arg Leu Leu Gln Asn Asn Tyr Ile Thr Gly Asn Ile Pro His Glu85 90 95att ggg aaa ttg atg aaa ctc aaa aca ctt gat ctc tct acc aat aac 393Ile Gly Lys Leu Met Lys Leu Lys Thr Leu Asp Leu Ser Thr Asn Asn100 105 110ttc act ggt caa atc cca ttc act ctt tct tac tcc aaa aat ctt cac 441Phe Thr Gly Gln Ile Pro Phe Thr Leu Ser Tyr Ser Lys Asn Leu His115 120 125agg agg gtt aat aat aac agc ctg aca gga aca att cct agc tca ttg 489Arg Arg Val Asn Asn Asn Ser Leu Thr Gly Thr Ile Pro Ser Ser Leu130 135 140 145gca aac atg acc caa ctc act ttt ttg gat ttg tcg tat aat aac ttg 537Ala Asn Met Thr Gln Leu Thr Phe Leu Asp Leu Ser Tyr Asn Asn Leu150 155 160agt gga cca gtt cca aga tca ctt gcc aaa aca ttc aat gtt atg ggc 585Ser Gly Pro Val Pro Arg Ser Leu Ala Lys Thr Phe Asn Val Met Gly165 170 175aat tct cag att tgt cca aca gga act gag aaa gac tgt aat ggg act 633Asn Ser Gln Ile Cys Pro Thr Gly Thr Glu Lys Asp Cys Asn Gly Thr180 185 190cag cct aag cca atg tca atc acc ttg aac agt tct caa aga act aaa 681Gln Pro Lys Pro Met Ser Ile Thr Leu Asn Ser Ser Gln Arg Thr Lys195 200 205aac cgg aaa atc gcg gta gtc ttc ggt gta agc ttg aca tgt gtt tgc 729Asn Arg Lys Ile Ala Val Val Phe Gly Val Ser Leu Thr Cys Val Cys210 215 220 225ttg ttg atc att ggc ttt ggt ttt ctt ctt tgg tgg aga aga aga cat 777Leu Leu Ile Ile Gly Phe Gly Phe Leu Leu Trp Trp Arg Arg Arg His230 235 240aac aaa caa gta tta ttc ttt gac att aat gag caa aac aag gaa gaa 825Asn Lys Gln Val Leu Phe Phe Asp Ile Asn Glu Gln Asn Lys Glu Glu245 250 255atg tgt cta ggg aat cta agg agg ttt aat ttc aaa gaa ctt caa tcc 873Met Cys Leu Gly Asn Leu Arg Arg Phe Asn Phe Lys Glu Leu Gln Ser260 265 270gca act agt aac ttc agc agc aag aat ctg gtc gga aaa gga ggg ttt 921Ala Thr Ser Asn Phe Ser Ser Lys Asn Leu Val Gly Lys Gly Gly Phe275 280 285gga aat gtg tat aaa ggt tgt ctt cat gat gga agt atc atc gcg gtg 969Gly Asn Val Tyr Lys Gly Cys Leu His Asp Gly Ser Ile Ile Ala Val290 295 300 305aag aga tta aag gat ata aac aat ggt ggt gga gag gtt cag ttt cag 1017Lys Arg Leu Lys Asp Ile Asn Asn Gly Gly Gly Glu Val Gln Phe Gln310 315 320aca gag ctt gaa atg ata agc ctt gcc gtc cac cgg aat ctc ctc cgc 1065Thr Glu Leu Glu Met Ile Ser Leu Ala Val His Arg Asn Leu Leu Arg325 330 335tta tac ggt ttc tgt act act tcc tct gaa cgg ctt ctc gtt tat cct 1113Leu Tyr Gly Phe Cys Thr Thr Ser Ser Glu Arg Leu Leu Val Tyr Pro340 345 350tac atg tcc aat ggc agt gtc gct tct cgt ctc aaa gct aaa ccg gta 1161Tyr Met Ser Asn Gly Ser Val Ala Ser Arg Leu Lys Ala Lys Pro Val355 360 365ttg gat tgg ggc aca aga aag cga ata gca tta gga gca gga aga ggg 1209Leu Asp Trp Gly Thr Arg Lys Arg Ile Ala Leu Gly Ala Gly Arg Gly370 375 380 385ttg ctg tat ttg cat gag caa tgt gat cca aag atc att cac cgt gat 1257Leu Leu Tyr Leu His Glu Gln Cys Asp Pro Lys Ile Ile His Arg Asp390 395 400gtc aaa gct gcg aac ata ctt ctt gac gat tac ttt gaa gct gtt gtc 1305Val Lys Ala Ala Asn Ile Leu Leu Asp Asp Tyr Phe Glu Ala Val Val405 410 415gga gat ttc ggg ttg gct aag ctt ttg gat cat gag gag tcg cat gtg 1353Gly Asp Phe Gly Leu Ala Lys Leu Leu Asp His Glu Glu Ser His Val420 425 430aca acc gcc gtg aga gga aca gtg ggt cac att gca cct gag tat ctc 1401Thr Thr Ala Val Arg Gly Thr Val Gly His Ile Ala Pro Glu Tyr Leu435 440 445tca aca gga caa tct tct gag aag aca gat gtg ttc ggt ttc ggg att 1449Ser Thr Gly Gln Ser Ser Glu Lys Thr Asp Val Phe Gly Phe Gly Ile450 455 460 465ctt ctt ctc gaa ttg att act gga ttg aga gct ctt gaa ttc gga aaa 1497Leu Leu Leu Glu Leu Ile Thr Gly Leu Arg Ala Leu Glu Phe Gly Lys470 475 480gca gca aac caa aga gga gcg ata ctt gat tgg gta aag aaa cta caa 1545Ala Ala Asn Gln Arg Gly Ala Ile Leu Asp Trp Val Lys Lys Leu Gln485 490 495caa gag aag aag cta gaa cag ata gta gac aag gat ttg aag agc aac 1593Gln Glu Lys Lys Leu Glu Gln Ile Val Asp Lys Asp Leu Lys Ser Asn500 505 510tac gat aga ata gaa gtg gaa gaa atg gtt caa gtg gct ttg ctt tgt 1641Tyr Asp Arg Ile Glu Val Glu Glu Met Val Gln Val Ala Leu Leu Cys515 520 525aca cag tat ctt ccc att cac cgt cct aag atg tct gaa gtt gtg aga 1689Thr Gln Tyr Leu Pro Ile His Arg Pro Lys Met Ser Glu Val Val Arg530 535 540 545atg ctt gaa ggc gat ggt ctt gtt gag aaa tgg gaa gct tct tct cag 1737Met Leu Glu Gly Asp Gly Leu Val Glu Lys Trp Glu Ala Ser Ser Gln550 555 560aga gca gaa acc aat aga agt tac agt aaa cct aac gag ttt tct tcc 1785Arg Ala Glu Thr Asn Arg Ser Tyr Ser Lys Pro Asn Glu Phe Ser Ser565 570 575tct gaa cgt tat tcg gat ctt aca gat gat tcc tcg gtg ctg gtt caa 1833Ser Glu Arg Tyr Ser Asp Leu Thr Asp Asp Ser Ser Val Leu Val Gln580 585 590gcc atg gag tta tca ggt cca aga tgacaagaga aactatatga atggctttgg 1887Ala Met Glu Leu Ser Gly Pro Arg595 600gtttgtaaaa aa 189957601PRTArabidopsis thaliana 57Met Leu Gln Gly Arg Arg Glu Ala Lys Lys Ser Tyr Ala Leu Phe Ser1 5 10 15Ser Thr Phe Phe Phe Phe Phe Ile Cys Phe Leu Ser Ser Ser Ser Ala20 25 30Glu Leu Thr Asp Lys Val Val Ala Leu Ile Gly Ile Lys Ser Ser Leu35 40 45Thr Asp Pro His Gly Val Leu Met Asn Trp Asp Asp Thr Ala Val Asp50 55 60Pro Cys Ser Trp Asn Met Ile Thr Cys Ser Asp Gly Phe Val Ile Arg65 70 75 80Leu Tyr Arg Leu Leu Gln Asn Asn Tyr Ile Thr Gly Asn Ile Pro His85 90 95Glu Ile Gly Lys Leu Met Lys Leu Lys Thr Leu Asp Leu Ser Thr Asn100 105 110Asn Phe Thr Gly Gln Ile Pro Phe Thr Leu Ser Tyr Ser Lys Asn Leu115 120 125His Arg Arg Val Asn Asn Asn Ser Leu Thr Gly Thr Ile Pro Ser Ser130 135 140Leu Ala Asn Met Thr Gln Leu Thr Phe Leu Asp Leu Ser Tyr Asn Asn145 150 155 160Leu Ser Gly Pro Val Pro Arg Ser Leu Ala Lys Thr Phe Asn Val Met165 170 175Gly Asn Ser Gln Ile Cys Pro Thr Gly Thr Glu Lys Asp Cys Asn Gly180 185 190Thr Gln Pro Lys Pro Met Ser Ile Thr Leu Asn Ser Ser Gln Arg Thr195 200 205Lys Asn Arg Lys Ile Ala Val Val Phe Gly Val Ser Leu Thr Cys Val210 215 220Cys Leu Leu Ile Ile Gly Phe Gly Phe Leu Leu Trp Trp Arg Arg Arg225 230 235 240His Asn Lys Gln Val Leu Phe Phe Asp Ile Asn Glu Gln Asn Lys Glu245 250 255Glu Met Cys Leu Gly Asn Leu Arg Arg Phe Asn Phe Lys Glu Leu Gln260 265 270Ser Ala Thr Ser Asn Phe Ser Ser Lys Asn Leu Val Gly Lys Gly Gly275 280 285Phe Gly Asn Val Tyr Lys Gly Cys Leu His Asp Gly Ser Ile Ile Ala290 295 300Val Lys Arg Leu Lys Asp Ile Asn Asn Gly Gly Gly Glu Val Gln Phe305 310 315 320Gln Thr Glu Leu Glu Met Ile Ser Leu Ala Val His Arg Asn Leu Leu325 330 335Arg Leu Tyr Gly Phe Cys Thr Thr Ser Ser Glu Arg Leu Leu Val Tyr340 345 350Pro Tyr Met Ser Asn Gly Ser Val Ala Ser Arg Leu Lys Ala Lys Pro355 360 365Val Leu Asp Trp Gly Thr Arg Lys Arg Ile Ala Leu Gly Ala Gly Arg370 375 380Gly Leu Leu Tyr Leu His Glu Gln Cys Asp Pro Lys Ile Ile His Arg385 390 395 400Asp Val Lys Ala Ala Asn Ile Leu Leu Asp Asp Tyr Phe Glu Ala Val405 410 415Val Gly Asp Phe Gly Leu Ala Lys Leu Leu Asp His Glu Glu Ser His420 425 430Val Thr Thr Ala Val Arg Gly Thr Val Gly His Ile Ala Pro Glu Tyr435 440 445Leu Ser Thr Gly Gln Ser Ser Glu Lys Thr Asp Val Phe Gly Phe Gly450 455 460Ile Leu Leu Leu Glu Leu Ile Thr Gly Leu Arg Ala Leu Glu Phe Gly465 470 475 480Lys Ala Ala Asn Gln Arg Gly Ala Ile Leu Asp Trp Val Lys Lys Leu485 490 495Gln Gln Glu Lys Lys Leu Glu Gln Ile Val Asp Lys Asp Leu Lys Ser500 505 510Asn Tyr Asp Arg Ile Glu Val Glu Glu Met Val Gln Val Ala Leu Leu515 520 525Cys Thr Gln Tyr Leu Pro Ile His Arg Pro Lys Met Ser Glu Val Val530 535 540Arg Met Leu Glu Gly Asp Gly Leu Val Glu Lys Trp Glu Ala Ser Ser545 550 555 560Gln Arg Ala Glu Thr Asn Arg Ser Tyr Ser Lys Pro Asn Glu Phe Ser565 570 575Ser Ser Glu Arg Tyr Ser Asp Leu Thr Asp Asp Ser Ser Val Leu Val580 585 590Gln Ala Met Glu Leu Ser Gly Pro Arg595 60058154DNAArabidopsis thaliana/note="Arabidopsis thaliana RKS7 partial cDNA sequence" 58agcgaatata cttcttgatg actactgtga agctgtggtt ggcgattttg gtttagctaa 60actcttggat catcaagatt ctcatgtgac aaccgcggtt agaggcacgg tgggtcacat 120tgctccagag tatctctcaa ctggtcaatc ctct 15459692DNAArabidopsis thaliana/note="Arabidopsis thaliana RKS7 partial cDNA sequence" 59aacagatgtt ttttggcttt gggattcttc ttcttgagct tgtaaccgga caaggagctt 60ttgagtctgt taaagcggct aaccggaaag gtgtgatgct tgattgggtt aaaaagattc 120atcaagagaa gaaacttgag ctacttgtgg ataaagagtt gttgaagaag aagagctacg 180atgagattga gttagacgaa atggtaagag tagctttgtt gtgcacacag tacctgccag 240gacatagacc aaaaatgtct gaagttgttc gaatgctgga aggagatgga cttgcagaga 300aatgggaagc ttctcaaaga tcagacagtg tttcaaaatg tagcaacagg ataaatgaat 360tgatgtcatc ttcagacaga tactctgatc ttaccgatga ctctagttta cttgtgcaag 420caatggagct ctctggtcct agatgaaatc tatacatgaa tctgaagaag aagaagaaca 480tgcatctgtt tcttgaatca agagggattc ttgttttttt gtataataga gaggtttttt 540ggagggaaat gttgtgtctc tgtaactgta taggcttgtt gtgtaagaag ttattactgc 600acttagggtt aattcaaagt tctttacata aaaaatgatt agttgcgttg aatagaggga 660acactttggg agatttcatg tatgaaattt gg 6926051PRTArabidopsis thaliana/note="Arabidopsis thaliana RKS-7 partial protein sequence" 60Ala Asn Ile Leu Leu Asp Asp Tyr Cys Glu Ala Val Val Gly Asp Phe1 5 10 15Gly Leu Ala Lys Leu Leu Asp His Gln Asp Ser His Val Thr Thr Ala20 25 30Val Arg Gly Thr Val Gly His Ile Ala Pro Glu Tyr Leu Ser Thr Gly35 40 45Gln Ser Ser5061147PRTArabidopsis thaliana/note="Arabidopsis thaliana RKS-7 partial protein sequence" 61Gln Met Phe Phe Gly Phe Gly Ile Leu Leu Leu Glu Leu Val Thr Gly1 5 10 15Gln Gly Ala Phe Glu Ser Val Lys Ala Ala Asn Arg Lys Gly Val Met20 25 30Leu Asp Trp Val Lys Lys Ile His Gln Glu Lys Lys Leu Glu Leu Leu35 40 45Val Asp Lys Glu Leu Leu Lys Lys Lys Ser Tyr Asp Glu Ile Glu Leu50 55 60Asp Glu Met Val Arg Val Ala Leu Leu Cys Thr Gln Tyr Leu Pro Gly65 70 75 80His Arg Pro Lys Met Ser Glu Val Val Arg Met Leu Glu Gly Asp Gly85 90 95Leu Ala Glu Lys Trp Glu Ala Ser Gln Arg Ser Asp Ser Val Ser Lys100 105 110Cys Ser Asn Arg Ile Asn Glu Leu Met Ser Ser Ser Asp Arg Tyr Ser115 120 125Asp Leu Thr Asp Asp Ser Ser Leu Leu Val Gln Ala Met Glu Leu Ser130 135 140Gly Pro Arg14562534DNAArabidopsis thaliana/note="Arabidopsis thaliana RKS-9 partial cDNA sequence" 62gaaatggtaa gagtagcttt gttgtgcaca cagtacctgc caggacatag accaagagtg 60tctgaagttg ttcgaatgct ggaaggagat ggacttgcag agaagtggga agcttctcaa 120ggatcagaca gtgtttcaaa atgtagcaac aggataaatg

aagtgatgtc atcttcagac 180agatactctg atgttaccga tgactctagt ttacgtgtgc aagcaatgga gctctctggt 240cctagatgaa gtctatacat gaatctgaag aagaagaaga acatgcatct gtttcttgaa 300tcaagaggga ttcttgtttt tttgtataat agagaggttt tttggaggga aatgttgtgt 360ctctgtaact gtataggctt gttgtgtaag aagttattac tgcacttagg gttaagtcaa 420agttctttac ataagggggg attagttgcg ttgaatagag ggaacacttt gggagatttc 480atgtgtgaaa gttgggaagt catgtttgag aatgaaggtt atcttattat tgaa 5346399PRTArabidopsis thaliana/note="Arabidopsis thaliana RKS-9 partial protein sequence" 63Val Asp Lys Glu Leu Leu Lys Lys Lys Ser Tyr Asp Glu Ile Glu Leu1 5 10 15Asp Glu Met Val Arg Val Ala Leu Leu Cys Thr Gln Tyr Leu Pro Gly20 25 30His Arg Pro Arg Val Ser Glu Val Val Arg Met Leu Glu Gly Asp Gly35 40 45Leu Ala Glu Lys Trp Glu Ala Ser Gln Gly Ser Asp Ser Val Ser Lys50 55 60Cys Ser Asn Arg Ile Asn Glu Val Met Ser Ser Ser Asp Arg Tyr Ser65 70 75 80Asp Val Thr Asp Asp Ser Ser Leu Arg Val Gln Ala Met Glu Leu Ser85 90 95Gly Pro Arg64586DNAArabidopsis thaliana/note="Arabidopsis thaliana RKS-15 partial cDNA sequence" 64gtggataaag agttgttgaa gaagaagagc tacgatgaga ttgagttaga cgaaatggta 60agagtagctt tgttgtgcac acagtacctg ccaggacata gaccaagagt gtctgaagtt 120gttcgaatgc tggaaggaga tggacttgca gagaagtggg gaagcttctc aaggatcaga 180cagtgtttca aaatgtagca acaggataaa tgaagtgatg tcatcttcag acagatactc 240tgatgttacc gatgactcta gtttacgtgt gcaagcaatg gagctctctg gtcctagatg 300aagtctatac atgaatctga agaagaagaa gaacatgcat ctgtttcttg aatcaagagg 360gattcttgtt tttttgtata atagagaggt tttttggagg gaaatgttgt gtctctgtaa 420ctgtataggc ttgttgtgta agaagttatt actgcactta gggttaagtc aaagttcttt 480acataagggg ggattagttg cgttgaatag agggaacact ttgggagatt tcatgtgtga 540aagttgggaa gtcatgtttg agaatgaagg ttatcttatt attgaa 5866599PRTArabidopsis thaliana/note="Arabidopsis thaliana RKS-15 partial protein sequence" 65Val Asp Lys Glu Leu Leu Lys Lys Lys Ser Tyr Lys Glu Ile Glu Leu1 5 10 15Asp Glu Met Val Arg Val Ala Leu Leu Cys Thr Gln Tyr Leu Pro Gly20 25 30His Arg Pro Arg Val Ser Glu Val Val Arg Met Leu Glu Gly Asp Gly35 40 45Leu Ala Glu Lys Trp Glu Ala Ser Gln Gly Ser Asp Ser Val Ser Lys50 55 60Cys Ser Asn Arg Ile Asn Glu Val Met Ser Ser Ser Asp Arg Tyr Ser65 70 75 80Asp Val Thr Asp Asp Ser Ser Leu Arg Val Gln Ala Met Glu Leu Ser85 90 95Gly Pro Arg66396DNAArabidopsis thaliana/note="Arabidopsis thaliana RKS-16 partial cDNA sequence" 66aaagtacgtg gaagcagaag tggagcagct gatacgaatg gctctgctct gcactcaaag 60ttctgcaatg gaacgtccaa agatgtcaga agtagtgaga atgctggaag gagatggttt 120agctgagaga tgggaagaat ggcaaaagga ggagatgcca atacatgatt ttaactatca 180agcctatcct catgctggca ctgactggct catcccctat tccaagtccc ttatcgaagg 240cgattacccc tcgggtccaa gataaccttt tagaaagggt cttttcttgt gggttcttca 300acaagtatat atatagattg gtgaagtttt aagatgcaag agggggccat gcacttttga 360atatcacctc ctctataagt agtattgtgt ctcttg 3966787PRTArabidopsis thaliana/note="Arabidopsis thaliana RKS-16 partial protein sequence" 67Lys Tyr Val Glu Ala Glu Val Glu Gln Leu Ile Arg Met Ala Leu Leu1 5 10 15Cys Thr Gln Ser Ser Ala Met Glu Arg Pro Lys Met Ser Glu Val Val20 25 30Arg Met Leu Glu Gly Asp Gly Leu Ala Glu Arg Trp Glu Glu Trp Gln35 40 45Lys Glu Glu Met Pro Ile His Asp Phe Asn Tyr Gln Ala Tyr Pro His50 55 60Ala Gly Thr Asp Trp Leu Ile Pro Tyr Ser Lys Ser Leu Ile Glu Gly65 70 75 80Asp Tyr Pro Ser Gly Pro Arg85

Claims

1-31. (canceled)

32. A method for propagation of a plant from non-embryogenic plant starting material, the method comprising stimulating root and/or shoot initiation of said plant starting material by having at least one recombinant gene product or functional fragment thereof transiently present in said starting material, wherein a reduction of exogenous phytohormone to said culture as compared with conventional methods is achieved.

33. A method for propagation of a plant from non-embryogenic plant starting material, the method comprising stimulating root and/or shoot initiation of said plant starting material by having at least one recombinant gene product or functional fragment thereof transiently present in said starting material, and wherein no exogenous phytohormone is added to said culture.

34. A method according to claim 32 further comprising transforming a least part of said starting material with a nucleic acid encoding said gene product.

35. A method according to claim 32 wherein said culture comprises an in vitro culture.

36. A method according to claim 32 wherein said propagation comprises essentially seedless propagation.

37. A method according to claim 32 wherein said starting material comprises an individual plant cell, protoplast, explant or plant tissue.

38. A plant or plant material obtainable by a method according to claim 32.

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