Patent application number | Description | Published |
20110208478 | METHOD FOR EVALUATING FLUORESCENCE CORRELATION SPECTROSCOPY MEASUREMENT DATA - With the different methods of fluorescence correlation spectroscopy, physical and biological transport processes in or between cells in the microscopic range, for example diffusion processes, can be analyzed. For this purpose, correlations of the fluorescence measurement data are determined for different sample regions and mathematical transport models are adapted thereto. Erroneous fluorescence correlation analyses were previously identified on the basis of the properties of the adapted model function parameters and were discarded. The a-priori knowledge necessary for the identification had to be obtained in time-consuming series of tests. With the invention, sample properties can be determined in a simpler, quicker and more exact way from fluorescence correlations. A suitability degree for one or more regions of the sample is determined for a correlation evaluation, describing quantitatively the information content of the respective region, or the error to be expected from a correlation evaluation, and can thus already be used before a correlation evaluation as a criterion for filtering/selecting the respective region. In this way, elaborate correlation calculations can be dispensed with in non-informative sample regions. | 08-25-2011 |
20110267688 | Microscopy Method and Microscope With Enhanced Resolution - Method for enhancing the resolution of a microscope during the detection of an illuminated specimen and a microscope for carrying out the method, wherein in a first position, an illumination pattern is generated on the specimen, the resolution of which is preferably within the range of the attainable optical resolution of the microscope or higher, wherein a relative movement, preferably perpendicular to the direction of illumination, from a first into at least one second position of the illumination pattern on the specimen is generated at least once between the detection and the illumination pattern with a step width smaller than the resolution limit of the microscope and detection and storage of the detection signals take place both in the first and in the second position. | 11-03-2011 |
20120081536 | Microscope System, Microscopy Method and Storage Medium - A microscope system comprises a microscope for data acquisition and a computing device configured to control the microscope during data acquisition and/or to perform data processing of raw data captured by the microscope. The computing device is coupled to an optical output device. The microscope and the computing device are configured to perform the data acquisition and/or data processing based on values that are respectively set for each one of a plurality of adjustable parameters. The computing device selectively outputs graphics data via the optical output device as a function of an adjustable parameter selected from the plurality of adjustable parameters. The output graphics data are assigned to the selected adjustable parameter and represent an affect of the selected adjustable parameter on at least one step of a procedure upon which the data acquisition and/or the data processing is based. | 04-05-2012 |
20130068967 | Method for Generating a Microscope Image and Microscope - The invention relates to a method and a microscope for generating a microscopic image, wherein
| 03-21-2013 |
20140146376 | Light microscope and microscopy method - A light microscope having a specimen plane, in which a specimen to be examined is positioned, having a light source to emit illuminating light, having optical imaging means to convey the illuminating light into the specimen plane, having a first scanning means, with which an optical path of the illuminating light and the specimen can be moved relative to each other to produce an illumination scanning movement of the illuminating light relative to the specimen, having a detector means to detect specimen light coming from the specimen and having electronic means to produce an image of the specimen based on the specimen light detected by the detector means at different specimen regions. A second scanning means is present, with which it can be adjusted which specimen region can be imaged on a determined detector element. | 05-29-2014 |
20140176678 | Method for High-Resolution 3D-Localization Microscopy - A method for high-resolution 3D-localization microscopy of a sample having fluorescence emitters, in which the fluorescence emitters in the sample are excited to emit fluorescent radiation and the sample is displayed with spatial resolution in wide-field microscopy. Excitation is caused such that in reference to the spatial resolution at least some fluorescence emitters are isolated. A three-dimensional localization is determined in a localization analysis, which includes in the depth direction of the display a z-coordinate and a x-coordinate as well as a y-coordinate orthogonal in reference thereto, for each isolated fluorescence emitter showing a precision exceeding the local resolution. A table of localization imprecision is provided, which states the imprecision of the localization, regarding its z-coordinate as a function of the z-coordinate and a number of photons collected during imaging in the wide-field microscopy. Localization imprecision being determined for each localized fluorescence emitter by accessing the table of localization imprecision for the localization determined during the localization analysis. | 06-26-2014 |
20140184777 | HIGH-RESOLUTION LUMINESCENCE MICROSCOPY - A microscopy method for generating a high-resolution image ( | 07-03-2014 |
20140293037 | OPTICAL MICROSCOPE AND METHOD FOR EXAMINING A MICROSCOPIC SAMPLE - An optical microscope includes a first mask that has transmission regions that are separated from one another for the simultaneous generation of a plurality of illumination light beams from illumination light, for example, a first scanning device for generating a scanning motion of the illumination light beams and a sample holder. The optical microscope also includes a second mask with transmission regions separated from one another, which transmission regions are smaller than the transmission regions of the first mask in order to clip the illumination light beams, such that, through the scanning motion of the first scanning device, each of the illumination light beams can be successively passed onto different transmission regions of the second mask, and a second scanning device is provided for generating a scanning motion between the clipped illumination light beams and the sample holder. A method for examining a microscopic sample is also provided. | 10-02-2014 |
20150035964 | HIGH-RESOLUTION LUMINESCENCE MICROSCOPY - A microscopy method for producing a high-resolution image of a sample which includes furnishing the sample with a marker that emits statistically flashing luminescence radiation after excitation, or using a sample that has molecules that emit statistically flashing luminescence radiation after excitation. The sample is excited to luminescence in such a manner that the marker/molecules emit luminescence radiation flashing at a flash rate, wherein—the illumination is structured in such a manner that the flash rate varies locally and—the sample is repeatedly illuminated in different illumination states of the structured illumination. The luminescing sample is repeatedly imaged on a detector in each of the different illumination states. | 02-05-2015 |
20150077842 | HIGH-RESOLUTION SCANNING MICROSCOPY - A microscope and method for high resolution scanning microscopy of a sample, having: an illumination device for the purpose of illuminating the sample, an imaging device for the purpose of scanning at least one point or linear spot across the sample and of imaging the point or linear spot into a diffraction-limited, static single image below a reproduction scale in a detection plane. A detector device for detecting the single image in the detection plane for various scan positions is also provided. An evaluation device for the purpose of evaluating a diffraction structure of the single image for the scan positions is provided. The detector device has a detector array which has pixels and which is larger than the single image. At least one phase mask with a variable lateral profile of the phase influence is included in or near to the objective pupil, or in a plane which is conjugated to the objective pupil, for generating a spatial distribution of the illumination light and/or the detection light perpendicular to the optical axis, and/or in the direction of the optical axis. | 03-19-2015 |
20150085099 | High-Resolution Scanning Microscopy - Microscope and method for high resolution scanning microscopy of a sample, wherein
| 03-26-2015 |