Patent application number | Description | Published |
20100184128 | PRODUCTION OF PRODUCTS OF PHARMACEUTICAL INTEREST IN PLANT CELL CULTURES - The present invention relates to transgenic plant cell cultures comprising transgenic plant cells comprising a plurality of nucleic acids heterologous to said plant, each of said nucleic acids comprising a coding sequence encoding a pharmaceutical product of interest operably linked to one or more regulatory elements for directing expression of said coding sequence in said plant cell, said nucleic acids being stably integrated at or adjacent to native rDNA of said plant cell; methods of producing the transgenic plant cell culture; and methods of producing a pharmaceutical product of interest using the transgenic plant cell culture. | 07-22-2010 |
20100261884 | CLOSTRIDIUM PERFRINGENS ALPHA TOXIN PROTEINS - This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic | 10-14-2010 |
20100311168 | NANOPARTICLE MEDIATED DELIVERY OF SEQUENCE SPECIFIC NUCLEASES - Provided are methods for introducing a sequence specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. | 12-09-2010 |
20110093982 | USE OF DENDRIMER NANOTECHNOLOGY FOR DELIVERY OF BIOMOLECULES INTO PLANT CELLS - Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using dendrimers, and optionally one or more CPPs. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. | 04-21-2011 |
20110191899 | ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS - A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants. | 08-04-2011 |
20110247100 | PLANT PEPTIDE GAMMA-ZEIN FOR DELIVERY OF BIOMOLECULES INTO PLANT CELLS - A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny. | 10-06-2011 |
20110318348 | INDUCTION OF MUCOSAL IMMUNE RESPONSES BY MUCOSAL DELIVERY PENTABODY COMPLEX (MDPC) - The subject invention provides, for example, a novel approach to specifically induce intranasal and/or oral mucosal as well as humoral antibody response by administrating a mucosal delivery pentabody complex (MDPC). The MDPC is a complex formed by mixing a target antigen and a mucosal delivery pentabody (MDP) that has a strong affinity to the target antigen. The MDP is a fusion protein of a single domain antibody (sdAb; which binds to the target antigen specifically) to a pentamerization domain (which can include the B-subunit of an AB5 toxin family, including the B subunit of cholera toxin (CT) or heat-labile toxin (LT)). The pentamerization domain can self-assemble into a pentamer, through which a pentameric single domain antibody, or a pentabody, is formed. | 12-29-2011 |
20120023619 | LINEAR DNA MOLECULE DELIVERY USING PEGYLATED QUANTUM DOTS FOR STABLE TRASFORMATION IN PLANTS - Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules. | 01-26-2012 |
20120023620 | PRODUCTION OF FUNCTIONALIZED LINEAR DNA CASSETTE AND QUANTUM DOT/NANOPARTICLE MEDIATED DELIVERY IN PLANTS - Methods for introducing a functionalized linear nucleic acid cassette molecule of interest into a plant cell comprising a cell wall include use of nanoparticles. In some embodiments, the cell comprising a cell wall is a cultured plant cell. Methods include genetically or otherwise modifying plant cells and for treating or preventing disease in any plant, especially crop plants. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with functionalized linear nucleic acid cassette molecules. | 01-26-2012 |
20120244569 | QUANTUM DOT CARRIER PEPTIDE CONJUGATES SUITABLE FOR IMAGING AND DELIVERY APPLICATIONS IN PLANTS - Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. | 09-27-2012 |
20140090112 | FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS - A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed. | 03-27-2014 |
20140090113 | ENGINEERED TRANSGENE INTEGRATION PLATFORM (ETIP) FOR GENE TARGETING AND TRAIT STACKING - An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5′ and 3′ ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases. | 03-27-2014 |
20140090116 | FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS - Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed. | 03-27-2014 |
20140173783 | PRECISION GENE TARGETING TO A PARTICULAR LOCUS IN MAIZE - The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed. | 06-19-2014 |
20140298547 | DNA DETECTION METHODS FOR SITE SPECIFIC NUCLEASE ACTIVITY - The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease. | 10-02-2014 |
20140304853 | METHODS AND COMPOSITIONS FOR INTEGRATION OF AN EXOGENOUS SEQUENCE WITHIN THE GENOME OF PLANTS - Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes. | 10-09-2014 |
20150040267 | ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS - A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants. | 02-05-2015 |
20150067921 | FAD3 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS - A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed. | 03-05-2015 |