Okuzaki
Ayako Okuzaki, Miyagi JP
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20090217420 | Method for transformation using mutant acetolactate synthase gene - Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker. | 08-27-2009 |
Ayako Okuzaki, Tsukuba-Shi JP
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20150101081 | PLANT TRANSFORMANT, TRANSFORMATION METHOD FOR PLANT, AND VECTOR USED IN SAID METHOD - The present invention provides a method for transforming organelles having an own genomic DNA, the method enabling efficient production of highly homoplasmic plant cells, in which most of the organelles are transformed, and so forth. The method comprises the steps of: expressing in plant cells a fusion protein containing a function inhibiting factor of the organelles and a transit signal peptide to the organelles; introducing into the genomic DNA of the organelles of the plant cells an expression cassette comprising DNAs encoding a restoring factor of the organelles and a factor desired to be expressed in the organelles; and allowing the function inhibiting factor to destroy the organelles, in which the expression cassette is not introduced, in the plant cells. | 04-09-2015 |
Daisuke Okuzaki, Osaka JP
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20090312199 | TRACE mRNA AMPLIFICATION METHOD AND USE THEREOF - In one embodiment of the present invention, a method for amplifying a trace amount of mRNA is disclosed which can amplify a short mRNA and a long mRNA at a same efficiency level, regardless of how long a base sequence is, and to provide a method for use of such a method, a method of the present invention for amplifying a trace amount of mRNA includes the steps of: (i) adding a dummy RNA to a solution containing the trace amount of mRNA, so as to prepare a mixed solution; (ii) synthesizing an anti-sense DNA by reverse transcription, which uses the mixed solution as a template; (iii) synthesizing a sense DNA which is complementary to the anti-sense DNA thus synthesized, so as to generate a double strand DNA made of the sense DNA and the anti-sense DNA; (iv) ligating an RNA polymerase promoter sequence to the double strand DNA thus generated, on a sense DNA 5′ end side of the double strand DNA, so as to prepare a double strand DNA for amplification; and (v) amplifying, by using RNA polymerase, an RNA from the double strand DNA for amplification. | 12-17-2009 |
Hidenori Okuzaki, Kofu-Shi JP
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20120133243 | ACTUATOR AND ACTUATOR MANUFACTURING METHOD - There is provided an actuator including a displacement unit made of a mixture of a silicone-containing elastomer and an ionic liquid; and multiple electrodes provided to apply an electric field to a part or whole of the displacement unit. Here, the displacement unit is deformed by applying a voltage between the multiple electrodes. | 05-31-2012 |
20140070197 | METHOD FOR FORMING PATTERNED ORGANIC ELECTRODE - A method for forming a patterned organic electrode includes printing a toner on a surface of a substrate using a laser printer such that a reverse pattern formed of the toner is formed on the substrate, supplying a solution containing PEDOT and PSS onto the substrate having the reverse pattern formed of the toner such that the solution containing PEDOT and PSS is supplied into a region of the surface of the substrate not covered with the reverse pattern, drying the solution containing PEDOT and PSS supplied onto the substrate, and supplying onto the substrate a stripping solution containing a toner removing solvent which removes the toner and a high conductive solvent which selectively removes the PSS such that the reverse pattern formed of the toner is stripped from the substrate. | 03-13-2014 |
20140091263 | Novel Polyvinyl Sulfonic Acid, Production Method Thereof, and Use Thereof - The present invention relates to polyvinyl sulfonic acid comprising a vinyl sulfonic acid unit represented by a specific general formula (1), wherein the molar amount of sulfonic acid groups derived from vinyl sulfonic acid monomers with respect to the molar amount of total monomer units is 50.0 to 98.0 mol %, and the polyvinyl sulfonic acid has an absorbance of 0.1 or greater (aqueous solution: 0.2 mass %, cell length: 10 mm) in a wavelength range of 255 to 800 nm. | 04-03-2014 |
Hidenori Okuzaki, Kofu JP
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20120180475 | ACTUATOR AND SHEET-SHAPED ACTUATOR - An actuator | 07-19-2012 |
Sayaka Okuzaki, Wako-Shi JP
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20100213741 | VEHICLE BODY FLOOR STRUCTURE - Vehicle body floor structure has a floor tunnel provided on a laterally central portion of a vehicle body floor panel and extending in a front-rear direction of a vehicle body, a desired stored component being stored in the floor tunnel. The stored component has a passageway formed therein to extend in a width direction of the vehicle body. The floor structure also has a cross member passed through the passageway of the stored component, the stored component is supported on the cross member via a resilient member, and the cross member is fixedly fastened at its opposite ends to the inner surfaces of opposed side walls of the floor tunnel. | 08-26-2010 |
Yoko Okuzaki, Kanagawa JP
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20090066823 | SOLID-STATE IMAGE PICKUP DEVICE AND CLAMP CONTROL METHOD THEREFOR - In an analog front end (FE) IC chip having a CDS (Correlated Double Sampling) function and an AGC (Automatic Gain Control) function, a clamp circuit for clamping an output signal during a black reference signal period is equipped with a mechanism for suppressing the effect of noises contaminated from a power source, external circuits, etc. | 03-12-2009 |
20100271526 | Solid-state imaging device, method for driving the same, and camera system - A solid-state imaging device includes: a pixel unit in which pixels, each of which converts light into a pixel signal and accumulates the pixel signal in accordance with a light exposure period, are arranged in a predetermined color layout and first-color pixel horizontal rows containing first-color pixels and second-color pixel horizontal rows containing second-color pixels are alternately arranged in a vertical row direction; readout units that select n (n≧2) single-color pixel signals from the first-color or second-color pixels in the first-color or second-color pixel horizontal rows, perform 1/n thinning-out on the selected n pixel signals to reduce the number of pixel signals to 1/n, and read the resultant pixel signal for each of the colors; and column processing units that perform column processing on the pixel signals having undergone the 1/n thinning-out. | 10-28-2010 |