Madden, CA
Brendan Madden, Berkeley, CA US
Patent application number | Description | Published |
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20120268467 | SYSTEM FOR ARRANGING A PLURALITY OF RELATIONAL NODES INTO GRAPHICAL LAYOUT FORM - A system for arranging a plurality of nodes into graphical layout form partitions the nodes into a set of clusters from which a cluster graph is created. The cluster partitioning is based on the inter-nodal relationships, and cluster size depends on the number of nodes associated with each cluster. The clusters are laid out to avoid overlap, and cluster boundaries, within which nodes in a cluster are constrained, are generated. Nodes of each cluster as classified as either internal or boundary nodes, with boundary nodes being projected outward towards the boundary of the cluster. As an optional step, connection edges between nodes are routed to avoid overlap. | 10-25-2012 |
20150022527 | Graph drawing techniques improving readabiity and aesthetics for high-degree nodes - A computer-implemented method is provided of drawing a graph in which nodes are connected by edges, with the edges having a specified minimum spacing. A shape or image representing a node is displayed, and a bounding box enclosing the node is determined, the bounding box being sized to satisfy the specified minimum spacing in relation to the bounding box. Outside the bounding box, placement of edges incident to the node is determined so as to satisfy the specified minimum spacing. Inside the bounding box, the edges are extended nearer to the image without requiring that the specified minimum spacing be observed, and the edges incident to the node are displayed. | 01-22-2015 |
Brendan Madden, Berekely, CA US
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20140292763 | Graph drawing using precise shape clipping - A computer-implemented method is provided for drawing a graph in which multiple nodes are connected by multiple edges. An image is displayed representing a node, and the image is processed to distinguish image foreground pixels and image background pixel. An edge is displayed incident to the node, the edge extending over selected background pixels and stopping short of selected foreground pixels. There results a graph drawing that is more aesthetically pleasing. | 10-02-2014 |
Christopher Madden, Mountain View, CA US
Patent application number | Description | Published |
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20100111539 | TRANSCEIVER MODULE AND INTEGRATED CIRCUIT WITH DUAL EYE OPENERS - A transceiver module having integrated eye diagram opening functionality for reducing jitter is described. The transceiver module may include a transmitter eye opener and a receiver eye opener integrated in a single circuit. The transceiver module may also include serial control and various other integrated components. Other functionalities that may be integrated on the transceiver module include loopback modes, bypass features, bit error rate testing, and power down modes. | 05-06-2010 |
20110293285 | TRANSCEIVER MODULE AND INTEGRATED CIRCUIT WITH DUAL EYE OPENERS - A transceiver module having integrated eye diagram opening functionality for reducing jitter is described. The transceiver module may include a transmitter eye opener and a receiver eye opener integrated in a single circuit. The transceiver module may also include serial control and various other integrated components. Other functionalities that may be integrated on the transceiver module include loopback modes, bypass features, bit error rate testing, and power down modes. | 12-01-2011 |
Darcy Madden, La Mesa, CA US
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20090176976 | NITRILASES - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 07-09-2009 |
20120009633 | NITRILASES AND METHODS FOR MAKING AND USING THEM - The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid. | 01-12-2012 |
Darcy Madden, San Diego, CA US
Patent application number | Description | Published |
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20100086986 | Nitrilases and Methods for Making and Using Them - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 04-08-2010 |
20100273659 | Nitrilases, Nucleic Acids Encoding Them and Methods for Making and Using Them - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 10-28-2010 |
20110263839 | Nucleic Acids Encoding Nitrilases - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 10-27-2011 |
20130177949 | NITRILASES - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 07-11-2013 |
David Madden, Forest Lake, CA US
Patent application number | Description | Published |
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20150216703 | ANKLE FLEXIBLE SUPPORT SYSTEM - A moldable ankle flexible brace is disclosed. The flexible ankle brace may include a heat-moldable, multi-layer leg support portion, a heat-moldable, multi-layer foot support portion, a pair of hinges connecting the leg support portion to the foot support portion, an opening for receiving a user's ankle, and a closure mechanism. The flexible ankle brace may include a middle layer that is substantially stiff at a temperature below about 130° F. and moldable at temperatures above 130° F. Also disclosed is a method of using a flexible ankle brace to treat an ankle. The method may include, for example, heating the brace to above 130 ° F. placing the heated brace on a patient's ankle and molding the brace to conform to the shape of the patient's ankle. | 08-06-2015 |
20150216704 | RIGID ANKLE SUPPORT SYSTEM - A moldable rigid ankle brace is disclosed. The rigid stabilizing ankle brace includes a heat-moldable multi-layer housing having an opening for receiving a user's ankle, a footplate attached to the housing, and a closure mechanism. The multi-layer housing includes a middle layer, which is substantially stiff at a temperature below about 130° F. and moldable at temperatures between about 130° F. and 275° F. A method of stabilizing an ankle is also disclosed. The method may include providing a rigid stabilizing ankle brace, having a heat-moldable multi-layer housing having an opening for receiving a user's ankle, and a footplate removably attached to said housing, heating the ankle brace to between about 130° F. to about 275° F., and applying said heated ankle brace to the ankle of a patient in need thereof | 08-06-2015 |
Dawn Madden, Half Moon Bay, CA US
Patent application number | Description | Published |
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20120303472 | METHODS FOR PLACING, ACCEPTING, AND FILLING ORDERS FOR PRODUCTS AND SERVICES - Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer. | 11-29-2012 |
Hugo Madden, Palo Alto, CA US
Patent application number | Description | Published |
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20100048916 | HIGH-PURITY TEXAPHYRIN METAL COMPLEXES - Disclosed herein are the methods and compositions for the improved synthesis of texaphyrin metal complexes. The improved synthesis results in high-purity compositions of texaphyrin metal complexes in which more than about 98% of the texaphyrin metal complexes in the composition have the same structure and/or the same molecular weight. Further described herein are pharmaceutical compositions comprising such high-purity compositions, and the use of such high-purity compositions in the treatment of cancer and cardiovascular diseases and disorders. | 02-25-2010 |
20110046100 | Texaphyrin solutions and pharmaceutical formulations - A packaging system is described for a drug that provides protection from contamination, crystallization and/or degradation of the drug during storage of the system prior to its use. The packaging of the drug does not significantly absorb, react with, or otherwise adversely affect the therapeutic effectiveness of the drug or other excipients or components during storage of the system prior to its use. The packaging system is also used for preserving a drug, particularly a drug containing high-purity texaphyrin metal complexes, by providing a product packaging system that prevents, limits or otherwise controls degradation reactions that can result from exposure to oxygen and/or light. | 02-24-2011 |
20130157994 | HIGH-PURITY TEXAPHYRIN METAL COMPLEXES - Disclosed herein are the methods and compositions for the improved synthesis of texaphyrin metal complexes. The improved synthesis results in high-purity compositions of texaphyrin metal complexes in which more than about 98% of the texaphyrin metal complexes in the composition have the same structure and/or the same molecular weight. Further described herein are pharmaceutical compositions comprising such high-purity compositions, and the use of such high-purity compositions in the treatment of cancer and cardiovascular diseases and disorders. | 06-20-2013 |
James Madden, Simi Valley, CA US
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20140125866 | AUDIO/VIDEO COMPANION SCREEN SYSTEM AND METHOD - Systems and methods disclosed herein include systems and methods for delivering secondary content to a plurality of portable devices, the secondary content comprising events synchronized to primary content, the process including delivering an application to a plurality of portable display devices, the application configured to execute events on the portable display devices; and causing the events executed on the portable display devices to be synchronized with primary content. | 05-08-2014 |
John P. Madden, Palo Alto, CA US
Patent application number | Description | Published |
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20100022823 | METHODS AND DEVICES FOR TISSUE GRASPING AND ASSESSMENT - Devices, systems and methods are provided for stabilizing and grasping tissues such as valve leaflets, assessing the grasp of these tissues, approximating and fixating the tissues, and assessing the fixation of the tissues to treat cardiac valve regurgitation, particularly mitral valve regurgitation. | 01-28-2010 |
20120010461 | METHODS AND DEVICES FOR TISSUE GRASPING AND ASSESSMENT - Devices, systems and methods are provided for stabilizing and grasping tissues such as valve leaflets, assessing the grasp of these tissues, approximating and fixating the tissues, and assessing the fixation of the tissues to treat cardiac valve regurgitation, particularly mitral valve regurgitation. | 01-12-2012 |
20140066693 | METHODS AND DEVICES FOR TISSUE GRASPING AND ASSESSMENT - Devices, systems and methods are provided for stabilizing and grasping tissues such as valve leaflets, assessing the grasp of these tissues, approximating and fixating the tissues, and assessing the fixation of the tissues to treat cardiac valve regurgitation, particularly mitral valve regurgitation. | 03-06-2014 |
John P. Madden, Redwood City, CA US
Patent application number | Description | Published |
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20090177266 | METHODS, SYSTEMS AND DEVICES FOR CARDIAC VALVE REPAIR - Disclosed are methods, systems, and devices for the endovascular repair of cardiac valves, particularly the atrioventricular valves which inhibit back flow of blood from a heart ventricle during contraction. The procedures described herein can be performed with interventional tools, guides and supporting catheters and other equipment introduced to the heart chambers from the patient's arterial or venous vasculature remote from the heart. The interventional tools and other equipment may be introduced percutaneously or may be introduced via a surgical cut down, and then advanced from the remote access site through the vasculature until they reach the heart. | 07-09-2009 |
20090326567 | LOCKING MECHANISMS FOR FIXATION DEVICES AND METHODS OF ENGAGING TISSUE - Devices and methods are provided for tissue approximation and repair. Fixation devices comprise a pair of elements each having a first end, a free end opposite the first end, and an engagement surface therebetween for engaging the tissue, the first ends being moveable between an open position wherein the free ends are spaced apart and a closed position wherein the free ends are closer together with the engagement surfaces generally facing each other. The fixation devices also include a locking mechanism coupled to the elements for locking the elements in place. The devices and methods find use in a variety of therapeutic procedures, including endovascular, minimally-invasive, and open surgical procedures, and can be used in various anatomical regions. The invention is particularly useful in procedures requiring minimally-invasive or endovascular access to remote tissue locations, where the instruments utilized must negotiate long, narrow, and tortuous pathways to the treatment site. | 12-31-2009 |
20100298929 | METHODS, SYSTEMS AND DEVICES FOR CARDIAC VALVE REPAIR - Disclosed are methods, systems, and devices for the endovascular repair of cardiac valves, particularly the atrioventricular valves which inhibit back flow of blood from a heart ventricle during contraction. The procedures described herein can be performed with interventional tools, guides and supporting catheters and other equipment introduced to the heart chambers from the patient's arterial or venous vasculature remote from the heart. The interventional tools and other equipment may be introduced percutaneously or may be introduced via a surgical cut down, and then advanced from the remote access site through the vasculature until they reach the heart. | 11-25-2010 |
John P. Madden, Emerald Hills, CA US
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20130103064 | INTEGRATED ABLATION AND MAPPING SYSTEM - A system for ablating and mapping tissue comprises a stand alone tissue ablation system adapted to ablate the tissue, and a stand alone cardiac mapping system adapted to map the tissue. The ablation system is operably coupled with the cardiac mapping system such that mapping data from the cardiac mapping system is provided to the ablation system to create a graphical display of the tissue and the ablation system position relative to the tissue. Motion of the ablation system may be monitored and adjusted based on feedback provided by ablation system actuators as well as position sensors. | 04-25-2013 |
20140046313 | TISSUE NECROSIS METHODS AND APPARATUS - Apparatus and methods for creating tissue necrosis include an energy delivery apparatus that can be positioned adjacent a target treatment site such as a vessel without direct contact with the treatment site tissue. Collimated energy is then directed to the vessel to create necrotic regions in the tissue. Exemplary use in renal vessels creates necrotic regions in adjacent nerves which can alleviate hypertension in a patient. | 02-13-2014 |
Joseph Madden, Livermore, CA US
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20140337041 | Mobile Application for Defining, Sharing and Rewarding Compliance with a Blood Glucose Level Monitoring Regimen - The invention provides a mobile application, system, interface and method for monitoring, encouraging and rewarding user compliance with a blood glucose level monitoring regimen. It combines a server-client based application enabling scheduling of tasks, input of data, reminders, notifications, permission based sharing of data, synchronous messaging, rewards based on compliance, and community support cross-language and cross-platform. The client application is a mobile web application (such as an iphone application) installed on a network connected mobile or desktop device. The client application may also be a web site that is stored on a server and accessed through a network such as the internet. The server and mobile applications exchange data with one another through the network using standard networking and wireless protocols. The application defines and exchanges information according to permission based rules which can be monitored and modified remotely by a parent or guardian. | 11-13-2014 |
Joseph Madden, San Francisco, CA US
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20150161574 | STEWARDSHIP METHOD FOR MANAGING OZONE-DEPLETING SUBSTANCES - A stewardship system integrates the destruction of ozone depleting substances, such as chlorofluorocarbons (CFC), carbon credit generation, sale of carbon credits within the various carbon credit markets, and distribution of the profits to the contributing entities. A central processing entity mediates transactions between the different entities. | 06-11-2015 |
20150254613 | CLOSED LOOP REFRIGERANT RECYCLING METHOD - A central recycling system manages refrigerant recycling by storing parameters describing a financial contribution from at least one participating entity to an incentive fund, receiving data about a used refrigerant-based appliance surrendered by a consumer, receiving data about disassembly of the used refrigerant-based appliance including recovery of refrigerant, receiving data about a recycling process applied to the recovered refrigerant, and determining a point of sale value associated with recycling the used refrigerant-based appliance based on the received data and the stored parameters. The recycling includes at least one of reclaiming and destroying the recovered refrigerant. | 09-10-2015 |
Joseph M. Madden, Los Gatos, CA US
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20110206378 | HAND CLEANLINESS - Among other things, systems to encourage compliance with hand washing procedures can include: an infrared emitter that projects a first infrared beam with a transverse cross-section having a first axis and a second axis that is shorter than the first axis, the transverse cross-section having a maximum length along the first axis, the infrared emitter modulating the first infrared beam to transmit a first identification signal; wherein the infrared emitter projecting the first infrared beam is placed such that the first axis of the transverse cross-section of the first infrared beam is substantially parallel to a boundary. | 08-25-2011 |
20140049391 | Hand Cleanliness - Among other things, systems to encourage compliance with hand washing procedures can include: an infrared emitter that projects a first infrared beam with a transverse cross-section having a first axis and a second axis that is shorter than the first axis, the transverse cross-section having a maximum length along the first axis, the infrared emitter modulating the first infrared beam to transmit a first identification signal; wherein the infrared emitter projecting the first infrared beam is placed such that the first axis of the transverse cross-section of the first infrared beam is substantially parallel to a boundary. | 02-20-2014 |
Judith Madden, Valencia, CA US
Patent application number | Description | Published |
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20090203017 | Use of Nucleic Acid Probes to Detect Nucleotide Sequences of Interest in a Sample - The invention relates to methods for the determination and detection of nucleic acids sequences in a sample. The nucleic acid may be RNA or DNA or both. The invention also relates to methods for the determination of the presence and species of various microorganisms in a sample. We have also identified a set of oligonucleotide nucleic acid sequences within the rRNAs of Gram-negative organisms that facilitates both the broad identification of Gram-negative organisms as a class when used as a pool, or in combination, for example in a hybridization assay. This set of oligonucleotides may detect sequences that are indicative of the presence of organisms of the broad class of Gram-negative organisms while exhibiting little or no false identification of Gram-positive organisms, and fungi, or other microorganisms. The assay includes concurrent incubation with at least one nucleotide sequence of interest, at least one nucleic acid probe, a fluorosurfactant, and a nuclease. The assay may further be employed to detect the presence of bacteria, fungi, or other microorganisms by use of additional specific probes, or to detect and/or identify target nucleic acid sequences in a sample. Further, the invention also relates to methods of reducing non-specific binding and facilitating complex formation in a binding assay. The binding assay may be, but is not limited to, a nucleic acid hybridization assay or an immunoassay. The invention also relates to methods of detection that employ at least one target of interest, which may be a nucleotide sequence, at least one probe, which may be a nucleic acid probe and a nuclease. | 08-13-2009 |
Kathleen A.d. Madden, Calabafas, CA US
Patent application number | Description | Published |
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20120095423 | Brassiere liner - A disposable bra liner has three layers which before use are flat, and in use become cup-shaped. The first layer is an outermost layer, the second layer is a middle layer, and the third layer is an innermost layer. The outermost layer and the innermost layer have circular peripheries and are disk-shaped. The middle layer has a central body portion and a plurality of arms extending outwardly in a generally spiral pattern. At least the outermost layer and the innermost layer are attached together, securing the middle layer between them. Regions of the first and third layers located between the spiral arms, when in use, become folded so as to allow contouring of the liner to the body of the user. | 04-19-2012 |
20140141693 | BRASSIERE LINER - A disposable bra liner has one embodiment with three layers which before use are flat, and in use become cup-shaped. The first layer is an outermost layer, the second layer is a middle layer, and the third layer is an innermost layer. The outermost layer and the innermost layer are disk-shaped. The middle layer has a central body portion and a plurality of arms extending outwardly in a generally spiral pattern. Regions of the first and third layers located between the spiral arms, when in use, become folded so as to allow contouring of the liner to the body of the user. In another embodiment, a further layer has a shape similar to that of the innermost layer, and is disposed between the outermost layer and the third layer to provide structural support. | 05-22-2014 |
Knut Madden, Carlsbad, CA US
Patent application number | Description | Published |
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20080241889 | METHODS AND COMPOSITIONS FOR SYNTHESIS OF NUCLEIC ACID MOLECULES USING MULTIPLE RECOGNITION SITES - The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention. | 10-02-2008 |
20130004996 | METHODS AND COMPOSITIONS FOR SYNTHESIS OF NUCLEIC ACID MOLECULES USING MULTIPLERECOGNITION SITES - The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according tb the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention. | 01-03-2013 |
20130157263 | METHODS AND COMPOSITIONS FOR SEAMLESS CLONING OF NUCLEIC ACID MOLECULES - The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention. | 06-20-2013 |
20130323796 | METHODS AND COMPOSITIONS FOR SYNTHESIS OF NUCLEIC ACID MOLECULES USING MULTIPLERECOGNITION SITES - The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination .sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a .number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention. | 12-05-2013 |
20130323797 | METHODS AND REAGENTS FOR MOLECULAR CLONING - The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3′ terminus of one end and has a single stranded 5′ overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5′ overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules. | 12-05-2013 |
20140142290 | COMPOSITIONS AND METHODS FOR PREPARING SHORT RNA MOLECULES AND OTHER NUCLEICACIDS - The invention provides methods of preparing nucleic acids, such as RNA molecules, of a defined size or range of sizes. The invention provides compositions, methods and kits for use in the production and preparation of small RNA molecules (including without limitation micro-RNA, siRNA, d-siRNA and e-siRNA) and other nucleic acids of various sizes. | 05-22-2014 |
20140170710 | METHODS AND COMPOSITIONS FOR SEAMLESS CLONING OF NUCLEIC ACID MOLECULES - The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention. | 06-19-2014 |
20150093788 | METHODS AND REAGENTS FOR MOLECULAR CLONING - The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3′ terminus of one end and has a single stranded 5′ overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5′ overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules. | 04-02-2015 |
20150152460 | METHODS AND COMPOSITIONS FOR SEAMLESS CLONING OF NUCLEIC ACID MOLECULES - The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention. | 06-04-2015 |
20150225729 | METHODS AND COMPOSITIONS FOR SYNTHESIS OF NUCLEIC ACID MOLECULES USING MULTIPLERECOGNITION SITES - The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention. | 08-13-2015 |
Knut R. Madden, Carlsbad, CA US
Patent application number | Description | Published |
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20090197307 | COMPOSITIONS AND METHODS FOR PREPARING SHORT RNA MOLECULES AND OTHER NUCLEIC ACIDS - The invention provides methods of preparing nucleic acids, such as RNA molecules, of a defined size or range of sizes. The invention provides compositions, methods and kits for use in the production and preparation of small RNA molecules (including without limitation micro-RNA, siRNA, d-siRNA and e-siRNA) and other nucleic acids of various sizes. | 08-06-2009 |
20090326208 | Methods and compositions for generating recombinant nucleic acid molecules - A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods. | 12-31-2009 |
20090328244 | METHODS AND REAGENTS FOR MOLECULAR CLONING - The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3′ terminus of one end and has a single stranded 5′ overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5′ overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules. | 12-31-2009 |
20110046201 | METHODS AND COMPOSITIONS FOR SEAMLESS CLONING OF NUCLEIC ACID MOLECULES - The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention. | 02-24-2011 |
20120108804 | COMPOSITIONS AND METHODS FOR PREPARING SHORT RNA MOLECULES AND OTHER NUCLEIC ACIDS - The invention provides methods of preparing nucleic acids, such as RNA molecules, of a defined size or range of sizes. The invention provides compositions, methods and kits for use in the production and preparation of small RNA molecules (including without limitation micro-RNA, siRNA, d-siRNA and e-siRNA) and other nucleic acids of various sizes. | 05-03-2012 |
20120129225 | METHODS AND REAGENTS FOR MOLECULAR CLONING - The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3′ terminus of one end and has a single stranded 5′ overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5′ overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences. | 05-24-2012 |
20120149069 | METHODS AND COMPOSITIONS FOR SEAMLESS CLONING OF NUCLEIC ACID MOLECULES - The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention. | 06-14-2012 |
20160024511 | YEAST PROMOTERS FOR PROTEIN EXPRESSION - Isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are disclosed. More specifically, nucleic acids isolated from | 01-28-2016 |
Mark Madden, La Mesa, CA US
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20090162906 | NITRILASES AND METHODS FOR MAKING AND USING THEM - The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid. | 06-25-2009 |
20090176976 | NITRILASES - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 07-09-2009 |
Mark Madden, San Diego, CA US
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20100086986 | Nitrilases and Methods for Making and Using Them - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 04-08-2010 |
20110263839 | Nucleic Acids Encoding Nitrilases - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 10-27-2011 |
20130177949 | NITRILASES - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 07-11-2013 |
Michael Madden, Mountain View, CA US
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20150022916 | SYSTEMS AND METHODS FOR CALIBRATING READ AND WRITE OPERATIONS IN TWO DIMENSIONAL MAGNETIC RECORDING - Systems and methods are provided for calibrating signals retrieved from a storage device using a first reader and a second reader. The systems and methods further include reading a first signal using the first reader and a second signal using the second reader. Control circuitry computes a calibration metric associated with the first reader and the second reader based on the combination of the first signal and the second signal. At least one of the first signal and the second signal is subsequently decoded based in part on the computed calibration metric. | 01-22-2015 |
Mike R. Madden, Mount Vernon, CA US
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20160096051 | Aircraft Fire Suppression System and Method - A fire suppression system for an aircraft having a compartment, the fire suppression system including an inert gas source in selective fluid communication with the compartment and a fire suppression agent source in selective fluid communication with the compartment, wherein an inert gas from the inert gas source and a fire suppression agent from the fire suppression agent source are at least partially combined to form a fire suppression mixture. | 04-07-2016 |
Pat Madden, Sunol, CA US
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20140368960 | OVER-VOLTAGE TOLERANT CIRCUIT AND METHOD - Over-voltage tolerant circuits and methods are provided. In one embodiment, the circuit includes a pull-up transistor coupled to an I/O pad, a sensing circuit coupled to the I/O pad and to a voltage supply (Vcc), the sensing circuit configured to sense a voltage applied to the pad (Vpad), a latch coupled to the sensing circuit to retain an output of the sensing circuit, and a selection circuit coupled to the sensing circuit through the latch. The selection circuit includes a first bias circuit to apply Vcc to a well and gate of the pull-up transistor, a second bias circuit to apply Vpad to the gate and the well of the pull-up transistor, and a non-overlap circuit configured to ensure the gate and the well of the pull-up transistor is substantially always driven by either the first or the second bias circuit depending on the output of the sensing circuit. | 12-18-2014 |
Sean Christopher Madden, Mission Viejo, CA US
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20140100518 | SYSTEMS AND METHODS FOR EXTERNAL PRESSURE SENSING - In various embodiments, a device for delivering pressurized irrigation may include a squeeze plate and a pressure sensor module. The squeeze plate may move relative to the pressure sensor module to apply pressure to a flexible container (e.g., a bag of irrigation fluid) between the squeeze plate and the pressure sensor module. The pressure sensor module may include a pressure sensor to measure a force exerted on the pressure sensor module by the flexible container as pressure is applied to the flexible container from the squeeze plate. In some embodiments, the pressure sensor module may include a bag contact plate and the pressure sensor may measure a force exerted on the bag contact plate by the flexible container located between the bag contact plate and the squeeze plate. In some embodiments, the pressure sensor may sense a pressure associated with the flexible container without an intervening bag contact plate. | 04-10-2014 |
Thomas Madden, Redwood City, CA US
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20100223553 | Multi-Media Center for Computing Systems - Various “media-components” are provided in a “multi-media center.” In modular architecture, a module-controller communicates with media-modules provided for various media-components. A media-module can include or obtain data pertaining to a particular media-component, identify media-player(s), and access information related to their media. However, the media-modules are isolated from each other, and the module-controller effectively controls output generated in response to user input. A user interface library is provided for the media-modules. Media-modules can obtain a template or other tools from the library and construct their user interface (e.g., menus). Media-modules can also identify a media-player that can be initiated in response to user input. Subsequently, the media-controller forwards user input to the media-player. | 09-02-2010 |
20110243525 | Multimedia control center - Techniques and systems for centralized access to multimedia content stored on or available to a computing device are disclosed. The centralized access can be provided by a media control interface that receives user inputs and interacts with media programs resident on the computing device to produce graphical user interfaces that can be presented on a display device. | 10-06-2011 |
Thomas Michael Madden, Sunnyvale, CA US
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20100185982 | Rendering Icons Along A Multidimensional Path Having A Terminus Position - Icons are arranged in foreground background positions in an interface environment to define a multidimensional path extending from a terminus. The icons transition between the foreground position and the background positions along the multidimensional path. Each icon corresponds to a content-specific menu item. | 07-22-2010 |
20100235792 | Content Abstraction Presentation Along A Multidimensional Path - Content abstractions are emerged in to an ingress terminus of a multidimensional path and depth transitioned through the multidimensional path to an egress terminus. The content abstractions are eliminated at the egress terminus. | 09-16-2010 |
20110154394 | User Interface With Menu Abstractions And Content Abstractions - Media menu items are generated within a media interface environment. Media menu item abstractions are generated, one of the media menu item abstractions arranged in a foreground position, and one or more of the media menu item abstractions arranged in background positions in the media interface environment. Selection of a media menu items transitions to a corresponding content menu interface. | 06-23-2011 |
20120207444 | Transport Controls for a Media Device - A method of scanning content comprises receiving an input signal indicating that content being displayed is to be scanned, sensing a duration of the received input signal, and scanning the content at a rate based at least in part on the sensed duration of the received input. | 08-16-2012 |
20130167032 | MULTI-MEDIA CENTER FOR COMPUTING SYSTEMS - Various “media-components” are provided in a “multi-media center.” In modular architecture, a module-controller communicates with media-modules provided for various media-components. A media-module can include or obtain data pertaining to a particular media-component, identify media-player(s), and access information related to their media. However, the media-modules are isolated from each other, and the module-controller effectively controls output generated in response to user input. A user interface library is provided for the media-modules. Media-modules can obtain a template or other tools from the library and construct their user interface (e.g., menus). Media-modules can also identify a media-player that can be initiated in response to user input. Subsequently, the media-controller forwards user input to the media-player. | 06-27-2013 |
20140108998 | MULTIMEDIA CONTROL CENTER - Techniques and systems for centralized access to multimedia content stored on or available to a computing device are disclosed. The centralized access can be provided by a media control interface that receives user inputs and interacts with media programs resident on the computing device to produce graphical user interfaces that can be presented on a display device. | 04-17-2014 |
20150227272 | MULTIMEDIA CONTROL CENTER - Techniques and systems for centralized access to multimedia content stored on or available to a computing device are disclosed. The centralized access can be provided by a media control interface that receives user inputs and interacts with media programs resident on the computing device to produce graphical user interfaces that can be presented on a display device. | 08-13-2015 |
20150331562 | MULTIMEDIA CONTROL CENTER - Techniques and systems for centralized access to multimedia content stored on or available to a computing device are disclosed. The centralized access can be provided by a media control interface that receives user inputs and interacts with media programs resident on the computing device to produce graphical user interfaces that can be presented on a display device. | 11-19-2015 |
20160057378 | Transport Controls for a Media Device - A method of scanning content comprises receiving an input signal indicating that content being displayed is to be scanned, sensing a duration of the received input signal, and scanning the content at a rate based at least in part on the sensed duration of the received input. | 02-25-2016 |