Patent application number | Description | Published |
20080266440 | PREDICTIVE AUTOFOCUSING - A method of autofocusing includes capturing first, second and third images of a sample, at respective first, second and third sample distances and respective first, second and third lateral positions determined with respect to an objective; determining a quantitative characteristic for the first, second and third images; determining a primary sample distance based upon at least the quantitative characteristics for the first, second, and third images; and capturing a primary image of the sample at the primary sample distance and at a primary lateral position that is offset from the first, second and third lateral positions. | 10-30-2008 |
20090195688 | System and Method for Enhanced Predictive Autofocusing - In an imaging device having an objective and a stage for holding a sample to be imaged, a method for autofocusing is presented. The method includes determining a measured focus value corresponding to at least a first of a plurality of logical image segments. Further, the method includes imaging the first logical image segment using the measured focus value. The method also includes determining a predicted focus value for a second of the plurality of logical image segments using the measured focus value and a stored focus variation parameter. In addition, the method includes imaging the second logical image segment using the predicted focus value. | 08-06-2009 |
20100301230 | METHOD AND APPARATUS FOR ULTRAVIOLET SCAN PLANNING - The invention provides method for locating one or more substantially circular-shaped tissue sample positioned on a solid support. The method involves the steps of transmitting light of a preselected wavelength onto a tissue sample, wherein the light induces the tissue sample to autofluoresce, identifying the center location of the tissue sample using the autofluoresced light, correlating the coordinates of the center location of the tissue sample on the solid support using an x, y-coordinate system, and mapping the coordinates of the tissue sample on the solid support to differentiate tissue sample containing regions from blank regions on the solid support. In a second aspect, the invention provides an apparatus for locating one or more substantially circular-shaped tissue sample positioned on a solid support. | 12-02-2010 |
20110090326 | SYSTEM AND METHOD FOR IMAGING WITH ENHANCED DEPTH OF FIELD - A method for imaging is presented. The method includes acquiring a plurality of images corresponding to at least one field of view using an imaging device having an objective and a stage for holding a sample to be imaged. Furthermore, the method includes determining a figure of merit corresponding to each pixel in each of the plurality of acquired images, wherein the figure of merit comprises a discrete approximation to a gradient vector. The method also includes synthesizing a composite image based upon the determined figures of merit. | 04-21-2011 |
20110090327 | SYSTEM AND METHOD FOR IMAGING WITH ENHANCED DEPTH OF FIELD - A method for imaging is presented. The method includes acquiring a plurality of images corresponding to overlapping fields of view at a plurality of sample distances using an imaging device having an objective and a stage for holding a sample to be imaged. Moreover, the method includes determining a figure of merit corresponding to each pixel in each of the plurality of acquired images. The method also includes synthesizing a composite image based upon the determined figures of merit. | 04-21-2011 |
20110091125 | SYSTEM AND METHOD FOR IMAGING WITH ENHANCED DEPTH OF FIELD - A method for imaging is presented. The method includes acquiring a plurality of images corresponding to at least one field of view at a plurality of sample distances. Furthermore, the method includes determining a figure of merit corresponding to each pixel in each of the plurality of acquired images. The method also includes for each pixel in each of the plurality of acquired images identifying an image in the plurality of images that yields a best figure of merit for that pixel. Moreover, the method includes generating an array for each image in the plurality of images. In addition, the method includes populating the arrays based upon the determined best figures of merit to generate a set of populated arrays. Also, the method includes processing each populated array in the set of populated arrays using a bit mask to generate bit masked filtered arrays. Additionally, the method includes selecting pixels from each image in the plurality of images based upon the bit masked filtered arrays. The method also includes processing the bit masked arrays using a bicubic filter to generate a filtered output. Further, the method includes blending the selected pixels as a weighted average of corresponding pixels across the plurality of images based upon the filtered output to generate the composite image having an enhanced depth of field. | 04-21-2011 |
20130044933 | SYSTEM AND METHODS FOR GENERATING A BRIGHTFIELD IMAGE USING FLUORESCENT IMAGES - A method for generating a brightfield type image, which resembles a brightfield staining protocol of a biological sample, using fluorescent images is provided. The steps comprise acquiring two or more fluorescent images of a fixed area on a biological sample, mapping said fluorescent image into a brightfield color space, generating a brightfield image, and optionally applying a sharpening transformation correction. Also provided is an image analysis system for generating a brightfield type image of a biological sample using fluorescent images. | 02-21-2013 |
20130178392 | METHODS OF ANALYZING AN H&E STAINED BIOLOGICAL SAMPLE - Methods comprising the use probing multiple targets in a H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, observing the sample, removing the hematoxylin and partially removing the eosin by washing the sample, contacting the sample with a borate salt, and irradiating the sample to remove the residual eosin fluorescence. The method further includes the optionally performing the additional steps of binding at least one probe to one or more targets to the sample ,observing a signal from the probe and contacting the sample with a bleaching agent. The process of binding, observing and bleaching may be iteratively repeated. | 07-11-2013 |
20140267671 | REFERENCING IN MULTI-ACQUISITION SLIDE IMAGING - Referencing of image acquired in multiple rounds of imaging is disclosed. In certain implementations, a baseline round of images are acquired and registered to one another to establish a global transformation matrix. In a subsequent round of image acquisition, a limited number of field of view images are initially acquired and registered to the corresponding baseline images to solve for translation, rotation, and scale. The full set of baseline images is then acquired for the subsequent round and each image is pre-rotated and pre-scaled based on the transform determined for the subset of images. The pre-rotated, pre-scaled images are then registered using a translation-only transform. | 09-18-2014 |
20140270425 | IMAGE QUALITY ASSESSMENT OF MICROSCOPY IMAGES - Automated assessment of registration quality, focus, and area defects in sequentially acquired images, such as images acquired by a digital microscope, is disclosed. In one embodiment, acquired images are registered and whole-image defects are automatically detected based on a figure of merit generated by the registration process. In related implementations, area defects may be automatically detected by calculating correlations in localized image regions for images acquired in different imaging rounds. | 09-18-2014 |