Patent application number | Description | Published |
20080220521 | SHEET FOR GUIDING REGENERATION OF MESENCHYMAL TISSUE AND PRODUCTION METHOD THEREOF - To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell. | 09-11-2008 |
20090299475 | BONE GRAFT SUBSTITUTE - To provide a bone graft substitute having an appropriate absorption period in a living body and high osteoconductivity, the bone graft substitute contains a carbonate apatite and an osteoinductive factor, the osteoinductive factor is preferably at least one kind selected from a group including BMP (a bone morphogenetic protein), GDF (a growth differentiation factor), TGF-β (a transformation growth factor), FGF (a fibroblast growth factor), IGF (an insulin-like growth factor), PDGF (a platelet-derived growth factor), BDNF (a brain-derived nerve growth factor), and NGF (a nerve growth factor), and the bone graft substitute has open pores, preferably having a diameter of 50 to 1000 μm and/or a diameter of 0.001 to 5 μm, with porosity of 20 to 80%. | 12-03-2009 |
20110076770 | CULTURING METHOD OF MESENCHYMAL STEM CELL - To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 g/ml at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring. | 03-31-2011 |
20120183923 | DENTAL IMPLANT AND SURFACE TREATMENT METHOD OF DENTAL IMPLANT - A dental implant is made of a mono metal body from inside to outermost surface of the implant, and the implant surface has macro pores having an inner diameter with the level of several tens of μm, micro pores having an inner diameter of 1 to 2 μm, and nano discharge craters having an inner diameter with the level of several tens of nm. A surface treatment method of the implant includes steps of producing macro pores having an inner diameter with the level of several tens of μm by blast processing, producing micro pores having an inner diameter of 1 to 2 μm by acid treatment, and producing nano discharge craters having an inner diameter with the level of several tens of nm by anodic oxidation treatment. | 07-19-2012 |
20140127808 | POROUS CELL SCAFFOLD AND PRODUCTION METHOD THEREOF - For easily seeding cells in its scaffold, a porous cell scaffold is produced by steps of filling a guiding solution with kinematic viscosity being 50 to 450% of that of a culture medium, in a whole continuous small hole structure having hole diameters of 5 to 3200 μm and an average hole diameter of 50 to 1500 μm, of a sheet-shaped or block-shaped scaffold having a thickness of 2 mm or more, supplying thereafter a culture medium with cells being suspended to an upper side of the scaffold, sucking the guiding solution from a lower side of the scaffold by low suction force, and entering thereby the culture medium with cells being suspended into the whole small hole structure, where a water absorber such as a filter paper is preferably used for sucking the guiding solution by low suction force. | 05-08-2014 |
Patent application number | Description | Published |
20090186412 | POROUS CELL SCAFFOLD AND PRODUCTION METHOD THEREOF - For easily seeding cells in its scaffold, a porous cell scaffold is produced by steps of filling a guiding solution with kinematic viscosity being 50 to 450% of that of a culture medium, in a whole continuous small hole structure having hole diameters of 5 to 3200 μm and an average hole diameter of 50 to 1500 μm, of a sheet-shaped or block-shaped scaffold having a thickness of 2 mm or more, supplying thereafter a culture medium with cells being suspended to an upper side of the scaffold, sucking the guiding solution from a lower side of the scaffold by low suction force, and entering thereby the culture medium with cells being suspended into the whole small hole structure, where a water absorber such as a filter paper is preferably used for sucking the guiding solution by low suction force. | 07-23-2009 |
20090221076 | METHOD OF FABRICATING SHEET FOR CARTILAGE TISSUE REGENERATION - An objective of the present invention is to provide a production method of a sheet for regenerating a cartilage tissue, which uses a conventional sheet-shaped porous body comprising a biological absorbency synthetic high polymer, such as polylactic acid, polyglycolic acid and a copolymer of lactic acid and glycolic acid. The sheet for regenerating the cartilage tissue can differentiate chondrocytes or stem cells without culturing the cells by pressurizing such as a pressurizing culture like the conventional method, and can accumulate the cells in a supporting carrier with high efficiency. The sheet for regenerating the cartilage tissue is produced by the steps of; seeding chondrocytes or stem cells differentiating to the chondrocytes on a sheet-shaped porous body comprising a biological absorbency synthetic high polymer; taking the porous body into a culture liquid; applying acceleration of 100 to 1000 G to the porous body for a predetermined time; and culturing the porous body without applying the acceleration. | 09-03-2009 |
20090246873 | SCAFFOLD FOR TISSUE ENGINEERING AND PRODUCTION METHOD THEREOF - To provide a scaffold for tissue engineering which consists of a bioabsorbable polymer material to be absorbed in a biotissue, and holds strength of the whole scaffold while having a porosity proper for culturing cells inside thereof as well, a method for producing the scaffold for tissue engineering includes steps of dissolving a bioabsorbable polymer material with an organic solvent, drying the solution so as to produce a porous bioabsorbable polymer material having a porosity of 50 to 99%, covering the porous bioabsorbable polymer material with a bioabsorbable polymer material having a thickness of 0.01 to 5 mm, pores of 10 to 3000 μm diameter, a fracture strength of 0.05 to 0.15 MPa, and a volume of 15 to 90% with respect to the whole scaffold. | 10-01-2009 |
20090261492 | BLOCK-SHAPED SCAFFOLD FOR TISSUE ENGINEERING AND PRODUCTION METHOD THEREOF - A block-shaped scaffold for a tissue engineering with improved shape stability and less volume change in water is produce by the steps of approximate-uniformly mixing the particle-shaped material having 100 to 2000 μm diameter with a solution, where a biodegradable polymer is dissolved with an organic solvent, freezing, drying it to remove the solvent, pulverizing thus obtained intermediate product, dissolving it with a liquid, where the biodegradable polymer is not dissolved, to remove the particle-shaped material taking thus obtained intermediate product into a mold, and pressing and heating it, the scaffold having ununiform and continuous holes occupying 20 to 80% in a cross-section area in a three-dimensional network structure having a small hole structure with 5 to 50 μm diameter, elastic coefficient being 0.1 to 2.5 MPa, and volume change being 95 to 105% when dipping it in water for 24 hours. | 10-22-2009 |
20100158982 | SHEET FOR GUIDING REGENERATION OF MESENCHYMAL TISSUE AND PRODUCTION METHOD THEREOF - To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell. | 06-24-2010 |
20130156820 | PRODUCTION METHOD OF TISSUE REGENERATION MATERIAL AND TISSUE REGENERATION MATERIAL - Provided is a production method of a tissue regeneration material whereby a cell suspension can be seeded and cultured on a porous support having relatively large pores. The method comprises: contacting a porous body to a holding plate having a contact angle to water of 15 to 90°, the porous body being formed of a bioabsorbable polymer material, having a thickness of 2 to 100 mm, an interconnected porous structure with a pore diameter of 180 to 3500 μm and an average pore diameter of 350 to 2000 μm, a porosity of 60 to 95%, and a compressive strength of 0.05 to 1 MPa, and being filled with a cell suspension having a cell density of 5×10 | 06-20-2013 |
20130259838 | CULTURED CARTILAGE TISSUE MATERIAL - Provided is a cultured cartilage tissue material which enables formation of cartilage tissue being substantially the same as the cartilage tissue actually in the living body, and which is large in volume. The cultured cartilage tissue material is to be used for cartilage regeneration and comprises chondrocytes, and is a mixed body of chondrocytes in a concentration of 1×10 | 10-03-2013 |
20140341859 | METHOD FOR TREATMENT OF DAMAGED SITE OF BONE - Provided is a treatment method which enables regeneration of bone tissue even when the size of a damaged site of bone is large. The treatment method comprises the steps of: culturing chondrocytes which have been seeded onto a porous body, or differentiating stem cells having chondrogenic differentiation potential which have been seeded onto a porous body into chondrocytes and culturing the chondrocytes; and implanting the porous body having the cultured chondrocytes. | 11-20-2014 |
20140371748 | FRACTURE TREATMENT DEVICE - Provided is a fracture treatment device enabling efficient bone regeneration. The fracture treatment device ( | 12-18-2014 |