Patent application number | Description | Published |
20080200349 | MICRODEVICES HAVING A PREFERENTIAL AXIS OF MAGNETIZATION AND USES THEREOF - This invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided. | 08-21-2008 |
20080220979 | Rapid Method To Detect Nucleic Acid Molecules - This invention relates to the field of detecting nucleic acid molecules using microarrays. The invention provides a method for detecting a target nucleic acid molecule in a biological sample by hybridizing a cell lysate directly probes immobilized on microarrays without any nucleic acid purification. | 09-11-2008 |
20080252954 | Laser Confocal Microarray Scanner - The invention provides a laser microarray scanner for microarray scanning, comprising an optical system, a scanning platform, and a data processing system. During scanning, the optical system remains fixed, and the microarray placed on the scanning platform moves relative to the optical system. The microarray scanner disclosed herein has high scanning speed, high sensitivity, high resolution, and high signal-to-noise ratio, thus is ideal for use in microarray scanning | 10-16-2008 |
20080267828 | Micro-Volume Liquid Ejection System - The present invention relates to a micro-volume liquid ejection system, including an air pressure module, a micro-ejection unit which is connected with the air pressure module by means of pipes, and a control circuit which is connected with the air pressure module and the micro-ejection unit respectively. In the present invention, due to air is used as the pressure medium, on one hand, as the sample does not contact with the pressure regulating module, the efficiency of cleaning process is improved, on the other hand, the volume of the sample needed in the sample ejecting process is only equal to the cavity-dimension of the micro-ejection unit by not need to fill the whole pipe with liquid. During the sample ejecting process, it is not need to regulate the pressure. After the sample ejecting is finished, the sample can be put into its original place, so as to greatly save the sample. The present invention is connected with the manipulator, so as to sample from the sample plate, eject sample and clean the pipes automatically, and it can also dispense a multiplicity of samples to micro-array substrate conveniently. The present invention can be used for transferring or dispensing the micro volume liquid of nL grade and L grade widely, and all kinds of the micro-volume liquid including biological liquid. | 10-30-2008 |
20090000948 | Methods for Improving Efficiency of Cell Electroporation Using Dielectrophoreses - The present invention provides methods for enhancing the efficiency of cell electroporation using dielectrophoresis-assisted cell localization and uses thereof in a microfluidic biochip system. Cells are first subject to dielectrophoresis and localized to regions where the electric field intensity is high enough to render cells electroporated. The invention enhances the efficiency of in situ cell electroporation on a traditional microfluidic biochip. | 01-01-2009 |
20090018025 | Testing Method of Nucleic Acid Binding Protein Based on Biochip - A testing method of nucleic acid binding protein based on biochip, comprises the following steps: 1. puts a plurality of groups solution including nucleic acid captured probes into biological sample including a plurality of nucleic acid binding protein to be test, and thus forming nucleic acid captured probe-nucleic acid binding protein complexes; such nucleic acid captured probe includes at least a segment of binding sequence which can bind with aimed nucleic acid binding protein; 2. separates such nucleic acid captured probe-nucleic acid binding protein complexes, then recoveries nucleic acid captured probes; 3. hybridizes the nucleic acid captured probes according to step 2 with a plurality of single strand blotting probes on biochip substrate; the sequence of such blotting probe compensates with such nucleic acid captured probe or one of its strand; 4. detects the result of hybridization. | 01-15-2009 |
20090039073 | METHODS AND DEVICES FOR CONTROLLING TEMPERATURE WITHOUT TEMPERATURE SENSOR - Methods and devices for controlling temperature by precise heating without the need of using a temperature sensor are provided. The device comprises a resistive heating element ( | 02-12-2009 |
20090046756 | Optical Systems for Microarray Scanning - The invention provides a novel optical system for use in a microarray scanner, comprising an aperture-containing reflecting mirror comprising an aperture and a reflecting surface. The aperture of the aperture-containing reflecting mirror allows an excitation light to pass through, and the reflecting surface of the aperture-containing reflecting mirror allows emission light from a microarray to be reflected. The optical system may also comprises various other components such as laser generators, beam splitter, reflecting mirrors, excitation and emission light filters, excitation and emission objective lens, pinhole, and detector. The optical system described herein has high efficiency, high sensitivity, low background noise, structurally simple, and high versatility. | 02-19-2009 |
20090101805 | High Speed Scanning Platform for Microarray Scanner - The invention provides a scanning platform for high speed scanning of microarrays. The platform uses a novel flexible a metal strip/wheel linear driving system to convert rotary movement of motors into linear movement, thereby drives movement of a stage/microarray in the direction of scanning. The platform of the present invention provides high movement speed, high resolution, and low return deviation. It is also simple in structure and low in manufacturing cost. | 04-23-2009 |
20090117561 | DIFFERENTIAL EXPRESSION GENE PROFILES AND APPLICATIONS IN MOLECULAR STAGING OF HUMAN GASTRIC CANCER - The invention provides methods for detecting differential gene expression in intestinal gastric tissue in a mammal by comparing the expression of specific genes in an intestinal gastric tissue suspected of being cancerous with that of the corresponding adjacent intestinal gastric tissue or a normal gastric mucosa tissue. The methods can be used in diagnosing or monitoring the progression of intestinal gastric cancer and determining the levels of differentiation of intestinal gastric cancer Systems and kits for methods of the invention are also provided. | 05-07-2009 |
20090123338 | BUBBLE-RESISTANT INJECTOR PORT FOR FLUIDIC DEVICES - An bubble-resistant injector port for fluidic and microfluidic devices includes an air-exhaustion feature to reduce the inclusion of bubbles or voids in injected samples, particularly in samples injected by a micropipette. The air-exhaustion feature comprises an air-exhaustion cavity in gas communication with the injector port through a narrowed channel that permits a flow of air into the cavity, while impeding a flow of injected liquid into the cavity. | 05-14-2009 |
20090137411 | Methods and biochips for detecting small molecule compounds - The present invention discloses methods for detection of small molecule compounds and its specific biochips. Biochips of the present invention comprise a solid support and carrier-linked small molecules immobilized onto the solid support. The invention also provides methods and kits for detection of small molecule compounds using the biochips of the invention. | 05-28-2009 |
20090143245 | MICROARRAYS FOR GENOTYPING AND METHODS OF USE - The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided. | 06-04-2009 |
20090148349 | WICKING INHIBITOR FOR FLUIDIC DEVICES - An wicking inhibitor for fluidic and microfluidic devices reduces wicking by providing a structure that interrupts the flow of a working fluid through a fluidic channel interface having corner angles greater than ninety degrees. | 06-11-2009 |
20090156419 | HYBRIDIZATION METHODS USING NATURAL BASE MISMATCHES - The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions. | 06-18-2009 |
20090239758 | METHODS, MICROARRAY, AND KITS FOR DETECTION OF DRUG RESISTANCE GENES IN GRAM-NEGATIVE BACTERIA - The present invention provides kits and microarrays containing primer pairs for amplifying drug resistance genes and/or probes for detection of drug resistance genes. Also provided are methods of detecting drug resistance genes using kits and microarrays described herein. | 09-24-2009 |
20090251155 | On-chip cell migration detection - Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal. | 10-08-2009 |
20090301231 | Microvalve Controlled Precision Fluid Dispensing Apparatus with a Self-Purging Feature and Method for use - An apparatus and methods for the precise, repeatable dispensing of small, sample fluid volumes, especially as related to the printing of microarrays for biological and/or chemical testing. A pressure tuning module meters fluid volumes for aspiration and dispensation and builds pressure for fluid dispensation, in conjunction with a microvalve that precisely controls the volume of the fluid dispensed under the built pressure. A pressure source can be switched in line to efficiently purge the apparatus of residual sample fluid. A working fluid can be optionally aspirated into the system, prior to aspirating the sample fluid, in order to maximize sample fluid recovery. | 12-10-2009 |
20090322309 | Microelectrode Arrays - Among others things, techniques, systems, and apparatus are disclosed for recording electrophysiological signals. In one aspect, a microelectrode sensing device includes a printed circuit board (PCB), a chip unit electrically connected to the PCB, and a cell culture chamber positioned over the chip unit and sealed to the PCB with the chip unit between the PCB and the cell culture chamber. The chip unit includes a substrate; a conductive layer positioned over the substrate that includes one or more recording electrodes; an insulation layer positioned over the conductive layer; another conductive layer positioned over the insulation layer that includes positioning electrodes; and another insulation layer positioned over the other conductive layer. The recording and positioning electrodes are electrically independent so as to independently receive a stimulus signal at each recording electrode and positioning electrode and independently detect a sensed signal at each recording electrode. | 12-31-2009 |
20100024888 | FLUIDIC FLOW MERGING APPARATUS - The present invention provides a device for handling fluids, and more particularly a merging apparatus for fluidic and microfluidic devices utilizing passive valving in conjunction with a single sensor to uniformly blend contributory working fluid streams. Symmetric and non symmetric embodiments with and without branch channels are described. The present invention also provides methods for merging liquids using the device of the invention. | 02-04-2010 |
20100041563 | METHODS FOR IDENTIFICATION OF ALLELES - The invention provides a method for identification of alleles. In this method, genomic DNA is used as target. Multiple allele-specific PCR amplification are carried out with a group of primers comprising one or more allele-specific primers for a target gene, a universal primer, and a common primer; and a DNA polymerase without 5′ to 3′ exonuclease activity. The PCR products are hybridized with tag probes immobilized on a DNA chip. Results are determined based on the signal intensity and the position of the probe immobilized on the array. Each allele-specific primer comprises a unique tag sequence at the 5′ end. Each tag probe immobilized on the DNA chip comprises a sequence identical to its corresponding tag sequence; and each tag probe hybridizes only with the complementary sequence in the PCR amplification product. | 02-18-2010 |
20100068098 | MICROFLUIDIC DEVICES AND METHODS FOR MULTIPLE ANALYTE DETECTION - The invention is directed to microfluidic devices comprising at least two processing channels, wherein each of the processing channels comprises an inlet, an outlet, and a high-flow-resistant and hydrophilic conduit; a distributing channel, wherein the distributing channel comprises an upstream end and a downstream end, and is in fluid communication with each inlet of the processing channels via the high-flow-resistant and hydrophilic conduit; and a flushing channel, wherein the flushing channel comprises an upstream end and a downstream end, and is in fluid communication with each outlet of the processing channels. The invention also provides methods of using the microfluidic devices. | 03-18-2010 |
20100151448 | Asymmetric PCR Amplification, its Special Primer and Application - The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids. | 06-17-2010 |
20100190663 | DEVICE FOR WASHING AND HYBRIDIZATION OF BIOCHIPS - A device for biochip hybridization or washing is provided, which comprises a carousel ( | 07-29-2010 |
20100260984 | MICRODEVICES CONTAINING PHOTORECOGNIZABLE CODING PATTERNS AND METHODS OF USING AND PRODUCING THE SAME - This invention relates generally to the field of moiety or molecule analysis, isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a substrate; and b) a photorecognizable coding pattern on said substrate. Preferably, the microdevice does not comprise an anodized metal surface layer. Methods and kits for isolating, detecting and manipulating moieties, and synthesizing libraries using the microdevices are also provided. The invention further provides two-dimensional optical encoders and uses thereof. In certain embodiments, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided. | 10-14-2010 |
20100270176 | AUTOMATIC POSITIONING AND SENSING MICROELECTRODE ARRAYS - A microelectrode sensing device includes a substrate and an array of microelectrode sensors. Each sensor includes a first conductive layer that at least partially conducts electricity. The first conductive layer is formed above the substrate and patterned to include a recording electrode that measures electrical activities of target cells. Each sensor also includes a second conductive layer that at least partially conducts electricity. The second conductive layer is elevated above the first layer and patterned to include multiple positioning electrodes arranged to define a sensing region above the recording electrode. The positioning electrodes are designed to generate an electric field pattern in the sensing region to move and confine the target cells to a sub-region of the sensing region that at least partially overlaps the recording electrode. | 10-28-2010 |
20110177339 | FLUORESCENT MAGNETIC NANOPARTICLES AND PROCESS OF PREPARATION - This invention provides nanometer-sized fluorescent magnetic particles and processes of making them. The nanoparticle has a core particle comprising a magnetic material and a fluorescent material, and the particle size is less than about 1 micrometer. The nanoparticles can be coated with an inorganic or organic layer and can be surface-modified. The nanoparticles can be used in many biological assays. | 07-21-2011 |
20110301062 | RELIABLE FLUORESCENCE CORRECTION METHOD FOR TWO-COLOR MEASUREMENT FLUORESCENCE SYSTEM - Methods and devices use in two-color measurement systems. The methods and devices include methods of making corrections, methods of calculating correction factors, fluorescence scanners, and microarray chips. The said methods and devices enable a user to correct fluorescence intensities for errors caused by the occurrence of FRET and /or cross-talk when two fluorophores are used in two-color fluorescence arrays. | 12-08-2011 |
20110318226 | Calibration slide for fluorescence detection instruments and process of preparation - The present invention relates to calibration slides for fluorescence detection instruments and processing method of making them. The aim of this invention is to disclose calibration slides with high photostability and long lifetime for fluorescence detection instruments. The calibration slides are fabricated by patterning calibration spot arrays of modified inorganic phosphors on the glass slide. The process for producing a calibration slide comprises the following procedure: 1) Dispersing the inorganic phosphors of rare-earth doped complex in water; 2) Patterning the affay of the above suspension on the glass slide. The calibration slides in the present invention employ a very stable fluorescing material that is insensitive to photobleaching, has long lifetime and stability under mild storage condition. The calibration slides in the present invention can be used to calibrate and test for some fluorescence instruments, i.e. microarray scanner, fluorescent microscopy, fluorescence spectrometer, fluorescent multiwell-plate reader | 12-29-2011 |
20120202872 | METHODS AND COMPOSITIONS FOR THE DIAGNOSIS AND PROGNOSIS OF CERVICAL INTRAEPITHELIAL NEOPLASIA AND CERVICAL CANCER - The invention provides methods and compositions for the diagnosis and prognosis of cervical intraepithelial neoplasia and cervical cancer. The methods comprise the step of determining the expression levels or genetic status of specific miRNAs. | 08-09-2012 |
20120228386 | MICRODEVICES CONTAINING PHOTORECOGNIZABLE CODING PATTERNS AND METHODS OF USING AND PRODUCING THE SAME - This invention relates generally to the field of moiety or molecule analysis, isolation, detection and manipulation and library synthesis. In particular, the invention provides a microdevice, which microdevice comprises: a) a substrate; and b) a photorecognizable coding pattern on said substrate. Preferably, the microdevice does not comprise an anodized metal surface layer. Methods and kits for isolating, detecting and manipulating moieties, and synthesizing libraries using the microdevices are also provided. The invention further provides two-dimensional optical encoders and uses thereof. In certain embodiments, the invention provides a microdevice, which microdevice comprises: a) a magnetizable substance; and b) a photorecognizable coding pattern, wherein said microdevice has a preferential axis of magnetization. Systems and methods for isolating, detecting and manipulating moieties and synthesizing libraries using the microdevices are also provided. | 09-13-2012 |
20130203611 | AUTOMATIC SLIDE LOADING DEVICE FOR MICRO ARRAY SCANNER AND ITS METHODS OF USE - An automatic slide loading device for microarray scanner comprises slide holders ( | 08-08-2013 |
20130204076 | INTEGRATED MICROFLUIDIC DEVICE FOR SINGLE OOCYTE TRAPPING - An integrated microfluidic device and its usage are provided. The microfluidic device comprises an upper layer ( | 08-08-2013 |
20130206250 | BUBBLE-BASED MICROVALVE AND ITS USE IN MICROFLUIDIC CHIP - Provided are a bubble-based microvalve and a microfluidic chip using the microvalve. Also provided are methods of using the microvalve for manipulating fluid in a microfluidic channel by changing the volume and/or location of the gas in the microvalve. | 08-15-2013 |
20130210675 | COMPOSITIONS AND METHODS FOR CONTROLLED RELEASE OF BIOMOLECULES - Provided are compositions for controlled release of biomolecules, which comprise conjugates of polymers and biomolecules conjugated through non-covalent interactions. Also provided are methods for controlled release of biomolecules and their use in biochips. | 08-15-2013 |
20130217584 | MICROARRAY-BASED ASSAY INTEGRATED WITH PARTICLES FOR ANALYZING MOLECULAR INTERACTIONS - A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises coupling a target molecule to a particle and then binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. Coupled with microarray, the particles, themselves or further modified, facilitate the detection of results with non-expensive devices or even naked eyes. This technology enables the detection and interpretation of molecular interactions in an efficient and cost effective way. | 08-22-2013 |
20130244270 | MICROFLUIDIC DEVICE FOR CELL MOTILITY SCREENING AND CHEMOTAXIS TESTING - The present invention relates to a microfluidic device used for cell motility screening and chemotaxis testing which comprises microfluidic channels and chambers. Cells which can secret a chemoattractant or chemorepellent are selectively planted in the microfluidic device and a stable chemoattractant or chemorepellent gradient can be established in the channels. | 09-19-2013 |
20130267436 | LUMINOPHORE-LABELED MOLECULES COUPLED WITH PARTICLES FOR MICROARRAY-BASED ASSAYS - A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. This technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way. | 10-10-2013 |
20140073536 | METHODS AND COMPOSITIONS FOR DETECTING NON-HEMATOPOIETIC CELLS FROM A BLOOD SAMPLE - The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell. | 03-13-2014 |
20140128281 | MICRO-REACTOR ARRAY - The present disclosure provides a cover sheet for a microarray reaction device. In one aspect, the present cover sheet or device ensures the reaction units/volumes are stable and/or consistent among assay samples and assay runs, allowing samples (e.g., reaction solutions) to be conveniently added and distributed uniformly. | 05-08-2014 |
20140285215 | CELL-IMPEDANCE SENSORS - Systems, apparatus and methods for designing and operating a cell-electrode impedance sensor to detect chemical and biological samples, including biological cells. In one implementation, a method of designing a cell-electrode impedance sensor includes determining a cell free cell-electrode impedance and a cell covered cell-electrode impedance based on a design model for the cell-electrode impedance sensor, wherein the design model is based on one or more factors, the factors including properties and elements of a cell-electrode impedance measurement system, using the cell free cell-electrode impedance and the cell covered cell-electrode impedance to obtain a sensor sensitivity of the cell-electrode impedance measurement system, and choosing one or more design parameters of the cell-electrode impedance sensor in the cell-electrode impedance measurement system to maximize the sensor sensitivity. | 09-25-2014 |