Patent application number | Description | Published |
20090159842 | MODULAR LINKERS FOR CONJUGATION OF ORGANIC SUBSTANCES TO SUBSTANTIALLY INORGANIC SUBSTANCES AND METHODS OF MANUFACTURE AND USE THEREOF - A modular linker includes an inorganic binding entity having an affinity for a substantially inorganic substance, and an organic binding entity capable of binding with an organic substance covalently bonded thereto. The modular linker is capable of being stored in a stable condition for later use. The modular linker may be synthesized by modifying the inorganic binding entity to be covalently bonded to an organic binding entity and storing the modular linker in an inert environment from about a day up to at least 1 week. The modular linker may be conjugated to an organic substance and to a substantially inorganic substance in substantially a 1:1 ratio. The modular linker may have more than one organic binding entity covalently bonded to an inorganic binding entity or vice-versa. Also, a particular modular linker may have an organic binding entity capable of binding with a nucleic acid sequence. | 06-25-2009 |
20100075361 | METHODS OF GENERATING FLORESCENCE RESONANCE ENERGY TRANSFER (FRET) BETWEEN SEMICONDUCTOR QUANTUM DOTS AND FLUORESCENT DYES/PROTEINS VIA MULTI-PHOTON EXCITATION, ACHIEVING ZERO BACKGROUND OR DIRECT EXCITATION CONTRIBUTIONS TO THE FRET SIGNATURE - A system and method of sensing physiological conditions in biological applications includes a laser source for optically exciting a plurality of luminescent quantum dots and a plurality of biomolecules in a nanoscale sensing system having a nanocrystal structure, where the plurality of biomolecules is stained with dye. In a multi-photon excitation process, a laser system optically excites, the plurality of luminescent quantum dots and the plurality of biomolecules in the nanoscale sensing system, where fluorescence resonance energy transfer (FRET) occurs between the plurality of quantum dots and the plurality of biomolecules. Stability of self assembly of quantum dot peptide conjugates within the plurality of biomolecules is investigated. Physiological conditions at the cellular level are determined, using a spectrometer to sense fluorosence spectra. The sensing of physiological conditions includes transducing signals into immunoassays, clinical diagnostics and cellular imaging to provide treatment to biological subjects including human patients. | 03-25-2010 |
20110089241 | Orthogonal Charge-Based Spectral Coding with Quantum Dot Conjugates - Populations of quantum dots are combined with quantities of a modulator of photoluminescence to produce a plurality of optical barcodes having at least two distinguishable colors arising from varying quantities of a modulator of photoluminescence bound to the populations of quantum dots. | 04-21-2011 |
20110097797 | Modular Functional Peptides for the Intracellular Delivery of Nanoparticles - Described are peptides for delivery of a nanoparticle to the cytosol, the peptide comprising: (a) a nanoparticle association domain; (b) a proline-rich spacer domain; (c) an uptake domain; and (d) a vesicle escape domain comprising a non-hydrolyzable lipid moiety, wherein the spacer domain is between the nanoparticle association domain and the uptake and vesicle escape domains, and wherein the peptide, when attached to an extracellular nanoparticle, is effective to induce uptake of the nanoparticle by a cell and delivery of the nanoparticle to the cytosol of the cell. Also described are methods of delivery of a nanoparticle to the cytosol of a cell, the method comprising providing to a cell a nanoparticle attached to such a peptide. Exemplary nanoparticles include quantum dots. | 04-28-2011 |
20110098445 | COVALENT ATTACHMENT OF PEPTIDES AND BIOLOGICAL MOLECULES TO LUMINESCENT SEMICONDUCTOR NANOCRYSTALS - A method for covalent attachment of peptides to luminescent quantum dots or other inorganic nanoparticles. The first step in the method involves functionalizing at least a portion of a surface of the quantum dot or nanoparticle with one or more materials having at least one reactive functional group therein. Subsequently, a peptide having a reactive functional group is reacted with at least some of the quantum dot or nanoparticle reactive functional groups to covalently bond at least some of the peptide to the quantum dots or nanoparticles. Modifications of the basic method are disclosed which provide methods allowing customized fabrication of quantum dots having a variety of different functional properties and combinations of functional properties. Also disclosed are quantum dots and nanoparticles made by the methods of the present invention. | 04-28-2011 |
20120248409 | Simultaneous Modulation of Quantum Dot Photoluminescence using Orthogonal Fluorescence Resonance Energy Transfer (FRET) and Charge Transfer Quenching (CTQ) - Quantum dots are modified with varying amounts of (a) a redox-active moiety effective to perform charge transfer quenching, and (b) a fluorescent dye effective to perform fluorescence resonance energy transfer (FRET), so that the modified quantum dots have a plurality of photophysical properties. The FRET and charge transfer pathways operate independently, providing for two channels of control for varying luminescence of quantum dots having the same innate properties. | 10-04-2012 |
20130045499 | Compact Multifunctional Ligand to Enhance Colloidal Stability of Nanoparticles - A ligand design allows compact nanoparticle materials, such as quantum dots (QDs), with excellent colloidal stability over a wide range of pH and under high salt concentrations. Self-assembled biomolecular conjugates with QDs can be obtained which are stable in biological environments. Energy transfer with these ligands is maximized by minimizing distances between QDs/nanoparticles and donors/acceptors directly attached to the ligands or assembled on their surfaces. | 02-21-2013 |
20130129627 | Delivery of Nanoparticles to Neurons - A peptide attached to a nanoparticles (such as quantum dots) selectively directs the nanoparticles to neurons in a tissue or organism. | 05-23-2013 |
20130130296 | Modular Functional Peptides for Delivery of Nanoparticles - A peptide directs nanoparticles (such as quantum dots) to the plasma membrane of mammalian cells. A method of delivery of a nanoparticle to a plasma membrane of a cell includes providing to the cell a nanoparticle attached to a peptide configured to direct the nanoparticle the plasma membrane, and allowing the cell to take up the nanoparticle. The nanoparticle can be a FRET donor to an organic dye. | 05-23-2013 |
20130309671 | Spectro-Temporal Optical Encoding of Information Using a Time-Gated Fluorescence Resonance Energy Transfer (FRET) - Described herein is a time-gated, two-step FRET relay effective to provide temporal transference of a prompt FRET pathway, or provide spectro-temporal encoding analytical signals and other information. A FRET relay assembly includes a long lifetime FRET donor (for example, a lanthanide complex), a semiconductor quantum dot (QD) configured as an intermediate acceptor/donor in FRET, and a fluorescent dye configured as a terminal FRET acceptor, wherein the long lifetime FRET donor has an excited state lifetime of at least one microsecond and the QD and fluorescent dye each have excited state lifetimes of less than 100 nanoseconds. | 11-21-2013 |
20130323777 | Compact Multifunctional Ligand to Enhance Colloidal Stability of Nanoparticles - A ligand design allows compact nanoparticle materials, such as quantum dots (QDs), with excellent colloidal stability over a wide range of pH and under high salt concentrations. Self-assembled biomolecular conjugates with QDs can be obtained which are stable in biological environments. Energy transfer with these ligands is maximized by minimizing distances between QDs/nanoparticles and donors/acceptors directly attached to the ligands or assembled on their surfaces. | 12-05-2013 |
20140256073 | Simultaneous Modulation of Quantum Dot Photoluminescence using Orthogonal Fluorescence Resonance Energy Transfer (FRET) and Charge Transfer Quenching (CTQ) - Quantum dots are modified with varying amounts of (a) a redox-active moiety effective to perform charge transfer quenching, and (b) a fluorescent dye effective to perform fluorescence resonance energy transfer (FRET), so that the modified quantum dots have a plurality of photophysical properties. The FRET and charge transfer pathways operate independently, providing for two channels of control for varying luminescence of quantum dots having the same innate properties. | 09-11-2014 |
20140264262 | Concentric Forster Resonance Energy Transfer Relay for the Parallel Detection of Two Bio/Physicochemical Process - Described herein is a Förster (or fluorescence) resonance energy transfer (FRET) configuration with three energy transfer pathways between three luminescent components, where two of the energy transfer steps occur in sequence as a relay, and the first step of the relay is in competition with a third energy transfer process (energy transfer from the donor to the intermediary is in competition with energy transfer from the donor directly to the terminal acceptor). | 09-18-2014 |