Patent application number | Description | Published |
20090162900 | EXPRESSION VECTOR, METHODS FOR THE PRODUCTION OF HETEROLOGOUS GENE PRODUCTS AND FOR THE SELECTION OF RECOMBINANT CELLS PRODUCING HIGH LEVELS OF SUCH OF SUCH PRODUCTS - An expression vector for eukaryotic cells comprising a gene which codes for a protein of interest, functionally linked to a hamster-ubiquitin/S27a-promoter and a gene which codes for a fluorescent protein. Preferably the expression vector also contains an amplifiable selectable marker gene. | 06-25-2009 |
20100297697 | METHODS FOR INCREASING PROTEIN TITERS - The invention relates to methods of increasing the titre of a protein of interest in a cell as well as the improved production and purification of optimised biomolecules, one component of which is the domain C | 11-25-2010 |
20100311121 | REGULATORY NUCLEIC ACID ELEMENTS - The invention relates to DNA-sequences, especially transcription- or expression-enhancing elements (TE elements) and their use on an expression vector in conjunction with an enhancer, a promoter, a product gene and a selectable marker. | 12-09-2010 |
20110124108 | EPIGENETIC ENGINEERING - The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through reducing expression of NoCR proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. | 05-26-2011 |
20110189729 | NOVEL RECOMBINATION SEQUENCES - The present invention relates to novel nucleotide sequences, which are variants of att recombination sequences, involved in sequence-specific recombination of DNA in eukaryotic cells, whereby sequence specific recombination is performed by a bacteriophage lambda integrase Int. Such novel att recombination sequences being e.g. attP.b, attP.a, attL.a, attR.a and attR.b. The present invention further relates to a method of sequence-specific recombination of DNA in eukaryotic cells, comprising the introduction of a first DNA comprising a nucleotide sequence containing at least one recombination sequence into a cell, introducing a second DNA comprising a nucleotide sequence containing at least one further recombination sequence into a cell, and performing the sequence specific recombination by a bacteriophage lambda integrase Int, whereby at least one of said first or second DNAs is a novel att recombination sequences being e.g. attP.b, attP.a, attL.a, attR.a and attR.b. | 08-04-2011 |
20110201785 | METHOD FOR OPTIMIZING PROTEINS HAVING THE FOLDING PATTERN OF IMMUNOGLOBULIN - The invention relates to a method for optimizing the biophysical properties of molecules and derivatives of the Ig superfamily. The method is characterized in that as yet unrecognized helical structural elements with unknown structural, stability and folding roles have been identified as important determinants of correct and efficient structuring of antibody domains. The novel process for positively influencing the antibody properties and properties of other proteins that have the Ig folding pattern now consists of optimizing the properties of the short helical elements and in the transplantation of these elements between Ig domains. | 08-18-2011 |
20110250640 | METHOD OF RECLONING PRODUCTION CELLS - A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells. | 10-13-2011 |
20110281301 | THE SECRETORY CAPACITY IN HOST CELLS - The invention concerns the field of protein production and cell culture technology. It describes a method of producing a heterologous protein of interest in a cell comprising a. Increasing the expression or activity of a secretion enhancing gene, and b. Increasing the expression or activity of an anti-apoptotic gene, and c. Effecting the expression of said protein of interest, whereby the secretion enhancing gene is a gene encoding a protein whose expression or activity is induced during one of the following cellular processes: plasma-cell differentiation, unfolded protein response (UPR), endoplasmic reticulum overload response (EOR). | 11-17-2011 |
20110312029 | NOVEL REGULATORY ELEMENTS - The invention concerns novel regulatory elements as well as related vectors and cells. Furthermore, it relates to methods of improving expression of polypeptides from nucleic acids such as cloned genes and to the production of various polypeptides in host cells using said novel regulatory elements. Additionally, the invention relates to uses of said novel regulatory elements as insulators, in gene therapy or for improving host cell lines. | 12-22-2011 |
20120190065 | COMBINATORIAL ENGINEERING - The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through introduction of nucleic acids encoding UBF or reducing expression of NoRC proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. The invention further concerns a method of producing proteins using the cells generated by the described method. | 07-26-2012 |
20130011391 | ANTI-PSGL-1 ANTIBODIES AND USES THEREOF - Provided herein, in one aspect, are antibodies that immunospecifically bind to PSGL-1, polynucleotides comprising nucleotide sequences encoding such antibodies, and expression vectors and host cells for producing such antibodies. Also provided herein are kits and pharmaceutical compositions comprising antibodies that specifically bind to PSGL-1, as well as methods of treating a disorder or disease caused by or associated with increased proliferation and/or numbers of activated T cells using the antibodies described herein. | 01-10-2013 |
20130177943 | NOVEL REGULATORY ELEMENTS - The invention concerns novel regulatory elements as well as related vectors and cells. Furthermore, it relates to methods of improving expression of polypeptides from nucleic acids such as cloned genes and to the production of various polypeptides in host cells using said novel regulatory elements. Additionally, the invention relates to uses of said novel regulatory elements as insulators, in gene therapy or for improving host cell lines. | 07-11-2013 |
20130209449 | ANTI-PSGL-1 ANTIBODIES AND USES THEREOF - Provided herein, in one aspect, are antibodies that immunospecifically bind to PSGL-1, polynucleotides comprising nucleotide sequences encoding such antibodies, and expression vectors and host cells for producing such antibodies. Also provided herein are kits and pharmaceutical compositions comprising antibodies that specifically bind to PSGL-1, as well as methods of treating a disorder or disease caused by or associated with increased proliferation and/or numbers of activated T cells using the antibodies described herein. | 08-15-2013 |
20130210074 | EPIGENETIC ENGINEERING - The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through reducing expression of NoCR proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. | 08-15-2013 |
20130251708 | ANTI-PSGL-1 ANTIBODIES AND USES THEREOF - Provided herein, in one aspect, are antibodies that immunospecifically bind to PSGL-1, polynucleotides comprising nucleotide sequences encoding such antibodies, and expression vectors and host cells for producing such antibodies. Also provided herein are kits and pharmaceutical compositions comprising antibodies that specifically bind to PSGL-1, as well as methods of treating a disorder or disease caused by or associated with increased proliferation and/or numbers of activated T cells using the antibodies described herein. | 09-26-2013 |
20140234893 | HETEROLOGOUS INTRON WITHIN AN IMMUNOGLOBULIN DOMAIN - The invention concerns the field of recombinant gene engineering. It concerns novel introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes with heterologous introns, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel intron sequences as heterologous introns. | 08-21-2014 |
20140242637 | ARTIFICIAL INTRONS - The invention concerns the field of recombinant gene engineering. It concerns novel artificial introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel artificial intron sequences. | 08-28-2014 |
20140248660 | HETEROLOGOUS INTRON WITHIN A SIGNAL PEPTIDE - The invention concerns the field of recombinant gene engineering. It concerns novel introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes with heterologous introns, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel intron sequences as heterologous introns. | 09-04-2014 |
20140248665 | NOVEL INTRON SEQUENCES - The invention concerns the field of recombinant gene engineering. It concerns novel introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes with heterologous introns, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel intron sequences as heterologous introns. | 09-04-2014 |
Patent application number | Description | Published |
20110296146 | HARDWARE INSTRUCTIONS TO ACCELERATE TABLE-DRIVEN MATHEMATICAL FUNCTION EVALUATION - A set of instructions for implementation in a floating-point unit or other computer processor hardware is disclosed herein. In one embodiment, an extended-range fused multiply-add operation, a first look-up operation, and a second look-up operation are each embodied in hardware instructions configured to be operably executed in a processor. These operations are accompanied by a table which provides a set of defined values in response to various function types, supporting the computation of elementary functions such as reciprocal, square, cube, fourth roots and their reciprocals, exponential, and logarithmic functions. By allowing each of these functions to be computed with a hardware instruction, branching and predicated execution may be reduced or eliminated, while also permitting the use of distributed instructions across a number of execution units. | 12-01-2011 |
20140019719 | GENERALIZED BIT MANIPULATION INSTRUCTIONS FOR A COMPUTER PROCESSOR - Methods of bit manipulation within a computer processor are disclosed. Improved flexibility in bit manipulation proves helpful in computing elementary functions critical to the performance of many programs and for other applications. In one embodiment, a unit of input data is shifted/rotated and multiple non-contiguous bit fields from the unit of input data are inserted in an output register. In another embodiment, one of two units of input data is optionally shifted or rotated, the two units of input data are partitioned into a plurality of bit fields, bitwise operations are performed on each bit field, and pairs of bit fields are combined with either an AND or an OR bitwise operation. Embodiments are also disclosed to simultaneously perform these processes on multiple units and pairs of units of input data in a Single Input, Multiple Data processing environment capable of performing logical operations on floating point data. | 01-16-2014 |
20140208066 | VECTOR GENERATE MASK INSTRUCTION - A Vector Generate Mask instruction. For each element in the first operand, a bit mask is generated. The mask includes bits set to a selected value starting at a position specified by a first field of the instruction and ending at a position specified by a second field of the instruction. | 07-24-2014 |
20140208067 | VECTOR ELEMENT ROTATE AND INSERT UNDER MASK INSTRUCTION - A Vector Element Rotate and Insert Under Mask instruction. Each element of a second operand of the instruction is rotated in a specified direction by a specified number of bits. For each bit in a third operand of the instruction that is set to one, the corresponding bit of the rotated elements in the second operand replaces the corresponding bit in a first operand of the instruction. | 07-24-2014 |