Patent application number | Description | Published |
20100091287 | Method for Imaging a Sample Using a Microscope, and Microscope and Data Storage Center - The invention relates to a method for imaging a sample using a microscope, in particular a scanning microscope, in which the sample is illuminated with excitation light via an illuminating beam path, and light emitted from the sample is recorded via a detection beam path, wherein at least one adjustable beam splitter having an adjustable threshold wavelength is arranged in the detection beam path or/and in the illuminating beam path, and wherein light emitted from the sample is detected in at least one detection channel. According to the inventive method, for at least one predetermined sample region, a signal intensity of light detected in the at least one detection channel is recorded for a plurality of threshold wavelengths set at the adjustable beam splitter to obtain a signal/threshold-dependency of the predetermined sample region. | 04-15-2010 |
20100134881 | OBJECTIVE REPLACEMENT DEVICE FOR MICROSCOPES - The invention relates to an objective replacement device for a microscope, wherein the sample is located in a sample chamber and surrounded by an immersion medium within the sample chamber, means for positioning and aligning the sample relative to the focus of an objective being present, wherein the detection beam path is aligned horizontally, which is to say perpendicular to the direction of action of gravity. For an objective replacement device for a microscope of the type described above, according to the invention a device is provided for exchanging the objective, at the focus of which the sample is positioned and aligned, with at least another objective, the position and alignment of the sample within the sample chamber remaining the same. | 06-03-2010 |
20100177381 | Sample Holding System for a Microscope - The invention relates to a sample holding system for a microscope, comprising a sample chamber which has an upper opening and is filled with an immersion liquid, and in which a sample embedded in a transparent embedding compound is placed in a holder. The sample holding system also comprises means for the translatory movement of the sample in relation to a detection objective of the microscope, and means for rotating the sample about an essentially vertical rotational axis in a plane forming an angle different from zero with the optical axis of the detection objective. The means for rotating the sample in such a sample holding system comprise a rotational drive provided with a magnetic coupling or a belt drive and/or toothed wheel rotational drive arranged above the sample chamber, said drives transmitting the rotational movement to the holder, or the rotational movement is generated directly by the movement of the entire sample chamber. | 07-15-2010 |
20100193673 | OPTICAL ARRANGEMENT FOR PHOTOMANIPULATION - The invention is directed to an optical arrangement for photomanipulation of a sample comprising a sample holder for receiving the sample, an illumination device comprising an illumination light source and an illumination beam path for illuminating the sample with a light sheet. It further comprises a detection device for detecting light that is radiated from the sample and imaging optics which image the sample on the detection device by means of an imaging objective in an imaging beam path, wherein the light sheet is substantially planar in the focus of the imaging objective, and wherein the imaging objective has an optical axis which intersects the plane of the light sheet at an angle different from zero. Further, the arrangement also has means for photomanipulation of the sample. | 08-05-2010 |
20100239138 | METHOD FOR POSITIONING BIOLOGICAL SAMPLES IN A MICROSCOPIC ARRANGEMENT - The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices are proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation. | 09-23-2010 |
20100309548 | METHOD AND OPTICAL ASSEMBLY FOR ANALYSING A SAMPLE - A method and an arrangement for analyzing a specimen, wherein the specimen is supported so as to be rotatable around an axis of rotation and displaceable in all three spatial directions and is illuminated by a first illumination device. Light radiated from the specimen is imaged on a detection device. A plurality of sectional images of the specimen are recorded at different settings of the rotational angle, and the specimen is rotated. The recorded sectional images are fused to form a data set of spatial image data of the specimen. The specimen is then illuminated by a second illumination device perpendicular to the axis of rotation, wherein a plurality of shadow images of the specimen are recorded and the specimen is rotated. A second data set of spatial image data of the specimen is constructed from the recorded shadow images by means of a back projection algorithm. | 12-09-2010 |
20110031414 | DEVICE FOR MICROSCOPY HAVING SELECTIVE ILLUMINATION OF A PLANE - A microscopy device, particularly for use in an imaging fluorescence lifetime microscopy method is provided. The microscopy device comprises an illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined, an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector. The illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined. The illumination means comprise a pulsed laser. | 02-10-2011 |
20110036996 | APPARATUS AND METHOD FOR HIGH SPATIAL RESOLUTION IMAGING OF A STRUCTURE OF A SAMPLE - Apparatus and method for high spatial resolution imaging of a sample's structure, including a diffraction-limited resolution volume with a plurality of dye molecules which can be switched between different states and have a distribution density which is greater than the inverse of the diffraction-limited resolution volume, where at least one state is fluorescing, the fluorescence being collected by an objective lens and imaged on a spatially resolving detector by an optical system. At least one light source provided for emitting a switching radiation and for emitting an excitation radiation. At least one of the light sources is arranged to radiate through the sample, and a switching and/or fluorescence excitation of the dye molecules is carried out. The switching is a photoactivation or a photodeactivation of the dye molecules. A focusing arrangement is provided for switching and/or for excitation to generate a line-like illumination region extending in a direction of illumination. | 02-17-2011 |
20110170182 | DEVICE FOR HOLDING AND POSITIONING A SAMPLE RELATIVE TO A MICROSCOPE LENS - An arrangement for holding and positioning a sample in the detection area of the objective of a microscope, the detection area being located in a chamber which is filled with an immersion liquid. This arrangement includes (1) a sample holder to which the sample is affixed so as to lie upon a point P in a coordinate system X, Y, Z, coordinate Z being defined by the optical axis of the microscope objective and the coordinate origin laying within the detection area, (2) a device by which the position of point P can be varied within the coordinate system X, Y, Z, wherein the range of variation comprehends the detection area, and (3) a device for rotating the sample affixed to the sample holder around point P, wherein a straight line G which encloses with coordinate Z an angle α whose size can be changed is the axis of rotation. | 07-14-2011 |
20120200693 | MICROSCOPE WITH A SHEET OF LIGHT - A family of microscopes with illumination systems directing a sheet of light having an approximately planar extension in an illumination axis of an illumination beam path and in a transverse axis orthogonally oriented to the illumination axis. The microscopes have detection devices used to detect light that is emitted by a sample region. The detection devices including a detection lens system disposed in the detection beam path and an optical detection element spaced from a front lens of the detection lens system and independently adjustable thereof. The optical detection element continuously varies the size of a detection image field and/or continuously displaces a focal plane of detection in the P-region. | 08-09-2012 |
20120282667 | METHOD FOR POSITIONING BIOLOGICAL SAMPLES IN A MICROSCOPIC ARRANGEMENT - The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices arc proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation. | 11-08-2012 |