Patent application number | Description | Published |
20080220493 | COMPOSITE ELECTROPORATION PLATE WITH INTERCHANGEABLE WELL INSERTS - Electroporation on a plurality of samples of membranous structures is performed in an electroporation well plate that includes a frame that can hold a plurality of well strips to form a two-dimensional array of wells, and a set of well strips, the set containing strips that differ in the number of wells while having the same outer dimensions and hence being interchangeable, thereby allowing the user to select strips appropriate for a given electroporation procedure, and allowing the manufacturer to replace defective strips without rejecting an entire well plate when a small number of wells is found to be defective. | 09-11-2008 |
20090081750 | TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW - Biological cells and other membranous structures are transfected in a flow-through system by first rendering the structures magnetically active such that they respond to a magnetic field, suspending the structures in a solution of an exogenous species with which the structures are to be transfected, then placing the suspension in a channel and using a moving magnetization pattern along the channel wall to cause the structures to travel through the channel. Along their path of travel, the structures pass a transmitter that emits transfection energy sufficient to cause the exogenous species in the suspension to permeate the structure membranes and enter the interiors of the structures. | 03-26-2009 |
20090209017 | Electroporation Cuvette With Spatially Variable Electric Field - An electroporation cuvette is constructed with electroporation electrodes arranged in non-parallel relation to form a gap whose width varies with the location within the cuvette, plus a pair of positioning electrodes that are arranged to cause electrophoretic migration of biological cells within the cuvette according to cell size. Once the cells, suspended in a solution of the impregnant, are distributed in the cuvette by the positioning electrodes, electric field pulses are generated by the non-parallel electroporation electrodes. Because of their distribution in the cuvette, the various cells will experience voltage differentials across their widths that approach uniformity regardless of cell diameter, since the larger cells will be positioned at locations where the gap between the electrodes is greater and the smaller cells at locations where the gap is relatively small while the voltage drop across the entire gap is uniform along the length of the cell. The voltage differential across the width of the cell is thus roughly paired with the cell diameter, and this reduces the disparity in voltage differential that cells of different sizes would otherwise experience with parallel electrodes. | 08-20-2009 |
20090269851 | USE OF DISK SURFACE FOR ELECTROPORATION OF ADHERENT CELLS - Adherent cells on the surface of a disk are transfected by electroporation between coaxial circular cylinders with electrodes on the opposing surfaces on either side of the annular space between the cylinders by placing a buffer solution containing the transfecting species in the annular space over the cell and applying an electric potential between the electrodes. | 10-29-2009 |
20090305380 | ELECTROPORATION OF ADHERENT CELLS WITH AN ARRAY OF CLOSELY SPACED ELECTRODES - Adherent cells and other membranous structures that are immobilized on a solid surface are transfected by electroporation in which the electric field is produced by a array of closely spaced electrodes positioned above the surface. Each electrode is substantially smaller in at least one lateral dimension than the dimensions of a single cell, and the electrodes in each pair are spaced apart by distances selected such that that a maximum of one cell will reside within the field produced by each pair, and the distance of the electrodes above the surface to which the cells are adherent is small enough to place the cell within the resulting electric field and yet great enough to avoid contact of the electrodes with the cell membrane. | 12-10-2009 |
20090317883 | INDIVIDUAL-CELL ELECTROPORATION USING AREA-FOCUSED ELECTRIC FIELDS - Electroporation is performed on a population of cells, liposomes, vesicles, or other membrane-encased structures with uniform results regardless of size variations within the population, by drawing the membrane-encased structures into micron-sized openings that contain paired electrodes. An electric potential is then imposed between the paired electrodes to permeabilize only that portion of each cell that extends into the openings and resides within the electric field focused in the area between the electrodes. | 12-24-2009 |
20100041122 | CENTRIFUGAL FORCE-BASED SYSTEM FOR DETECTION/TREATMENT OF MEMBRANE-ENCASED STRUCTURES - Membrane-encased structures such as biological cells, liposomes, and vesicles, are conveyed through one or more channels in a rotating disk for individual exposure to optical elements or to electrodes, for purposes of transfection or flow cytometry. The rotation of the disk serves either to provide centrifugal force to urge the cells against one wall of the channel and in certain embodiments to move the cells through the channels, or to draw cells at preselected times or intervals into the exposure zone, or all three. | 02-18-2010 |
20100099581 | LOCALIZED TEMPERATURE CONTROL FOR SPATIAL ARRAYS OF REACTION MEDIA - Individual temperature control in multiple reactions performed simultaneously in a spatial array such as a multi-well plate is achieved by thermoelectric modules with individual control, with each module supplying heat to or drawing heat from a single region within the array, the region containing either a single reaction vessel or a group of reaction vessels. | 04-22-2010 |
20100297753 | TRANSFECTION BY LASER PORATION ON ROTATING CYLINDER - Biological cells are transfected by a laser poration process in which the structures are immobilized on a solid surface that is cylindrical in shape, and the cylinder, while immersed in a liquid solution of the transfecting species or otherwise in contact with the solution, is rotated past a stationary laser such that the laser beam spans the entire circumference of the cylinder and all cells immobilized thereon. Axial movement of the cylinder or the laser in addition to the rotational movement brings the entire length of the cylinder within the influence of the laser as well as the cylinder circumference. | 11-25-2010 |