Patent application number | Description | Published |
20100055039 | NON-INVASIVE METHODS OF MONITORING ENGRAFTED STEM CELLS AND METHODS FOR ISOLATION OF SKELETAL MUSCLE STEM CELLS - The embodiments of the present disclosure encompass methods for non-invasive in vivo bioluminescence imaging that allow the dynamics of stem cell behavior to be followed in a manner not possible using conventional retrospective static histological analyses. By imaging luciferase-generated bioluminescence activity emanating from isolated stem cells, for example, real time quantitative and kinetic analyses can show that donor-derived muscle stem cells may proliferate and engraft rapidly after injection until homeostasis is reached. In addition, the response of the stem cells to injury and participation in the regenerative response can be monitored over time. Other aspects of the disclosure encompasses methods for determining the suitability of a stem cell for tissue replacement, methods for repairing muscle injury, and methods for isolating muscle stem cells from a tissue sample. | 03-04-2010 |
20100055733 | MANUFACTURE AND USES OF REACTIVE MICROCONTACT PRINTING OF BIOMOLECULES ON SOFT HYDROGELS - Embodiments of the present disclosure encompass microfabrication methods (“reactive microcontact printing of soft matter”) for hydrated soft polymer materials and surfaces for culture platforms suitable for the culturing of isolated single primary mammalian cells in an environment approximating the natural niches of the cells. Such culture platforms may comprise arrays of microwells, or other microscopically textured features, in which individual features can comprise desired proteins or mixtures of proteins. The microfabrication methods of the disclosure allow spatial control of surface biochemistry and topography at the micrometer scale on these hydrated soft gels. The hydrogels and methods of manufacture and use of the disclosure allow the isolation of a single stem cell and the characterizing of its interaction with cytokines and morphogens, especially with regard to modulation of the proliferative capacity of the stem cell when implanted in a recipient host. The systems for isolating or culturing a eukaryotic cell comprise a hydrogel film comprising a cross-linked polymeric composition having the characteristic of hydrating to form a hydrogel and having a topographical feature or a plurality of topographical features that may have a surface capable of receiving and immobilizing at least one biomolecule species thereon. | 03-04-2010 |
20100055759 | TRANSPARENT POLYMER MEMBRANE FOR LASER DISSECTION - The disclosure encompasses transparent polymer membranes suitable for use in laser microdissection methods, where the membranes allow the subsequent analysis of the microdissected cell or cells with light of a wavelength that can traverse the membrane with little if any absorbance by the membrane. The transfer capture membranes of the disclosure comprise a polymeric composition having the characteristic of being cut by a laser light at a wavelength less than 360 nm, while being substantially optically transparent at a wavelength greater than about 360 nm. The disclosure further provides methods of isolating a cell or population of cells from a tissue, comprising: overlaying a tissue sample with a transfer capture membrane having the characteristic of being cut by a laser light at a wavelength less than 360 nm, and substantially optically transparent at a wavelength greater than about 360 nm, defining an area of the transfer capture membrane by a laser light cutting through the thickness of the transfer capture membrane and the issue sample; and removing the defined area of the transfer capture membrane with the targeted cell or population of cells adhering thereto. | 03-04-2010 |
20100285451 | DETECTION OF SUB-CELLULAR COMPARTMENT LOCALIZATION OF A MOLECULE USING A REDUCED AFFINITY ENZYME COMPLEMENTATION REPORTER SYSTEM - Methods and compositions for detecting the sub-cellular localization of a molecule are provided. Aspects of the invention include detecting translocation of a cell-surface receptor to a sub-cellular compartment, e.g., the endosome, using a reduced affinity enzyme complementation reporter system. Also provided are systems and kits for use in practicing embodiments of the methods. | 11-11-2010 |
20100300563 | Modular device and method for moving fluids to and from a sample delivery element - Provided is a sequenced fluid control device that includes a pneumatic driver module, a fluid cartridge having at least one fluid chamber and at least one waste chamber, and at least one sample delivery element disposed to sealably contact the fluid cartridge forming a main chamber containing a sample, where the fluid cartridge is also disposed to sealably contact the pneumatic driver module. The pneumatic driver module injects gas into or withdraws gas from the fluid cartridge, where the gas directly contacts at least one fluid causing at least one fluid to flow through channels disposed to connect at least one fluid chamber to the sample delivery element and disposed to connect the sample delivery element to the at least one waste chamber or waste channel, where a sample on the sample delivery element is exposed to the at least one fluid. | 12-02-2010 |
20120077204 | Detection of Protein Translocation by Beta-Galactosidase Reporter Fragment Complementation - Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between sub-cellular compartments, using at least two components that exhibit a localization-dependent difference in complementation activity. In particular, alpha-complementing β-galactosidase fragments are provided. These β-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is localized in a membrane. Methods for carrying out no-wash ELISA assays based on the reporter component system are also provided. | 03-29-2012 |
20120171174 | Methods of Inducing Tissue Regeneration - Methods are provided for producing cells within a lineage (lineage restricted cells) from post-mitotic differentiated cells of the same lineage ex vivo and in vivo, and for treating a subject in need of tissue regeneration therapy by employing these lineage-restricted cells. In addition, the production of lineage restricted cells from postmitotic tissues derived from patients with diseases allows for a characterization of pathways that have gone awry in these diseases and for screening of drugs that will ameliorate or correct the defects as a means of novel drug discovery. Also provided are kits for performing these methods. | 07-05-2012 |
20120177611 | Elastic substrates and methods of use in cell manipulation and culture - Methods are provided for the ex vivo manipulation of cells, stem cells and other reproductive cells, by manipulating the cells in a container or device comprising an elastic substrate, wherein the substrate has an elasticity that mimics the elasticity of a native microenvironment of the cell. | 07-12-2012 |
20120276064 | METHODS AND COMPOSITIONS FOR REJUVENATION AND EXPANSION OF STEM CELLS - The regenerative potential of stem cells is enhanced in vitro and in vivo by inhibition of the p38 MAPK signaling pathway. | 11-01-2012 |
20120295854 | Methods of Inducing Tissue Regeneration - Methods are provided for producing cells within a lineage (lineage restricted cells) from post-mitotic differentiated cells of the same lineage ex vivo and in vivo, and for treating a subject in need of tissue regeneration therapy by employing these lineage-restricted cells. In addition, the production of lineage restricted cells from postmitotic tissues derived from patients with diseases allows for a characterization of pathways that have gone awry in these diseases and for screening of drugs that will ameliorate or correct the defects as a means of novel drug discovery. Also provided are kits for performing these methods. | 11-22-2012 |
20130011380 | Use of Cytidine Deaminase-Related Agents to Promote Demethylation and Cell Reprogramming - Methods, compositions and kits for modulating demethylation in a mammalian cell are provided. Also provided are methods, compositions and kits for screening candidate agents for activity in modulating genomic DNA demethylation in mammalian cells. These methods, compositions and kits find use in producing induced pluripotent stem cells (iPS) and somatic cells in vitro and for treating human disorders including cancer and disorders arising from defects in genomic imprinting. | 01-10-2013 |
20130052267 | Methods of Inducing Tissue Regeneration - Methods are provided for producing cells within a lineage (lineage restricted cells) from post-mitotic differentiated cells of the same lineage ex vivo and in vivo, and for treating a subject in need of tissue regeneration therapy by employing these lineage-restricted cells. In addition, the production of lineage restricted cells from postmitotic tissues derived from patients with diseases allows for a characterization of pathways that have gone awry in these diseases and for screening of drugs that will ameliorate or correct the defects as a means of novel drug discovery. Also provided are kits for performing these methods. | 02-28-2013 |
20140178913 | Detection of Molecular Interactions Using a Reduced Affinity Enzyme Complementation Reporter System - Methods and compositions for detecting molecular interactions are provided. Aspects of the invention include the use of a reduced affinity enzyme complementation reporter system. Also provided are systems and kits for use in practicing embodiments of the methods. | 06-26-2014 |
20140242154 | Compounds, Compositions, Methods, and Kits Relating to Telomere Extension - Compounds and compositions for the transient expression of exogenous telomerase activity in a cell are provided. The compounds and compositions, which relate to a ribonucleic acid coding for a telomerase reverse transcriptase, are useful in the extension of telomeres in cells needing such treatment. Such cells include, for example, cells that contain shortened telomeres and cells from subjects that may benefit from telomere extension, for example subjects that suffer from, or are at risk of suffering from, age-related or other illnesses. Also provided are methods of extending telomeres through the administration of the provided compounds and compositions to animal cells, either in vitro or in vivo, and kits including the compounds or compositions and instructions for use. | 08-28-2014 |
20140242155 | Compounds, Compositions, Methods, and Kits Relating to Telomere Extension - Compounds and compositions for the transient expression of exogenous telomerase activity in a cell are provided. The compounds and compositions, which relate to a ribonucleic acid coding for a telomerase reverse transcriptase, are useful in the extension of telomeres in cells needing such treatment. Such cells include, for example, cells that contain shortened telomeres and cells from subjects that may benefit from telomere extension, for example subjects that suffer from, or are at risk of suffering from, age-related or other illnesses. Also provided are methods of extending telomeres through the administration of the provided compounds and compositions to animal cells, either in vitro or in vivo, and kits including the compounds or compositions and instructions for use. | 08-28-2014 |
20140370522 | DETECTION OF PROTEIN TRANSLOCATION BY BETA-GALACTOSIDASE REPORTER FRAGMENT COMPLEMENTATION - Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between sub-cellular compartments, using at least two components that exhibit a localization-dependent difference in complementation activity. In particular, alpha-complementing β-galactosidase fragments are provided. These β-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is localized in a membrane. Methods for carrying out no-wash ELISA assays based on the reporter component system are also provided. | 12-18-2014 |
20150037295 | METHODS OF INDUCING TISSUE REGENERATION - Methods are provided for producing cells within a lineage (lineage restricted cells) from post-mitotic differentiated cells of the same lineage ex vivo and in vivo, and for treating a subject in need of tissue regeneration therapy by employing these lineage-restricted cells. In addition, the production of lineage restricted cells from postmitotic tissues derived from patients with diseases allows for a characterization of pathways that have gone awry in these diseases and for screening of drugs that will ameliorate or correct the defects as a means of novel drug discovery. Also provided are kits for performing these methods. | 02-05-2015 |